LOCUS       DAZKSU010000001       108912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000001 DAZKSU010000000
VERSION     DAZKSU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 108912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 108912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..108912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.001"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..755
                     /locus_tag="ACG8Z3_000001"
     CDS             27..755
                     /locus_tag="ACG8Z3_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0822356.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(824..2347)
                     /locus_tag="ACG8Z3_000002"
     CDS             complement(824..2347)
                     /locus_tag="ACG8Z3_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0822357.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            2599..3780
                     /locus_tag="ACG8Z3_000003"
     CDS             2599..3780
                     /locus_tag="ACG8Z3_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0822358.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3840..5468)
                     /gene="treC"
                     /locus_tag="ACG8Z3_000004"
     CDS             complement(3840..5468)
                     /gene="treC"
                     /locus_tag="ACG8Z3_000004"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0822359.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5919..8630)
                     /locus_tag="ACG8Z3_000005"
     CDS             complement(5919..8630)
                     /locus_tag="ACG8Z3_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0822360.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(8640..9341)
                     /locus_tag="ACG8Z3_000006"
     CDS             complement(8640..9341)
                     /locus_tag="ACG8Z3_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822361.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            9527..10126
                     /locus_tag="ACG8Z3_000007"
     CDS             9527..10126
                     /locus_tag="ACG8Z3_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0822362.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            10207..11220
                     /locus_tag="ACG8Z3_000008"
     CDS             10207..11220
                     /locus_tag="ACG8Z3_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0822363.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            11328..12245
                     /gene="rbsK"
                     /locus_tag="ACG8Z3_000009"
     CDS             11328..12245
                     /gene="rbsK"
                     /locus_tag="ACG8Z3_000009"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0822364.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            12273..12668
                     /gene="rbsD"
                     /locus_tag="ACG8Z3_000010"
     CDS             12273..12668
                     /gene="rbsD"
                     /locus_tag="ACG8Z3_000010"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0822365.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            12668..14041
                     /locus_tag="ACG8Z3_000011"
     CDS             12668..14041
                     /locus_tag="ACG8Z3_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0822366.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            14398..16542
                     /locus_tag="ACG8Z3_000012"
     CDS             14398..16542
                     /locus_tag="ACG8Z3_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0822367.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(16594..17634)
                     /locus_tag="ACG8Z3_000013"
     CDS             complement(16594..17634)
                     /locus_tag="ACG8Z3_000013"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0822368.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17911..19713)
                     /locus_tag="ACG8Z3_000014"
     CDS             complement(17911..19713)
                     /locus_tag="ACG8Z3_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822369.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(19727..20479)
                     /locus_tag="ACG8Z3_000015"
     CDS             complement(19727..20479)
                     /locus_tag="ACG8Z3_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822370.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(20488..20850)
                     /locus_tag="ACG8Z3_000016"
     CDS             complement(20488..20850)
                     /locus_tag="ACG8Z3_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0822371.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYIQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(21026..21898)
                     /locus_tag="ACG8Z3_000017"
     CDS             complement(21026..21898)
                     /locus_tag="ACG8Z3_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0822372.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21949..22437)
                     /locus_tag="ACG8Z3_000018"
     CDS             complement(21949..22437)
                     /locus_tag="ACG8Z3_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0822373.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(22575..23366)
                     /locus_tag="ACG8Z3_000019"
     CDS             complement(22575..23366)
                     /locus_tag="ACG8Z3_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0822374.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(23421..24029)
                     /locus_tag="ACG8Z3_000020"
     CDS             complement(23421..24029)
                     /locus_tag="ACG8Z3_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0822375.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(24022..24339)
                     /locus_tag="ACG8Z3_000021"
     CDS             complement(24022..24339)
                     /locus_tag="ACG8Z3_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0822376.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(24495..25223)
                     /locus_tag="ACG8Z3_000022"
     CDS             complement(24495..25223)
                     /locus_tag="ACG8Z3_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0822377.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKSCGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            25382..26263
                     /locus_tag="ACG8Z3_000023"
     CDS             25382..26263
                     /locus_tag="ACG8Z3_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0822378.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(26500..27543)
                     /locus_tag="ACG8Z3_000024"
     CDS             complement(26500..27543)
                     /locus_tag="ACG8Z3_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0822379.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27546..28214)
                     /locus_tag="ACG8Z3_000025"
     CDS             complement(27546..28214)
                     /locus_tag="ACG8Z3_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0822380.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            28356..30692
                     /locus_tag="ACG8Z3_000026"
     CDS             28356..30692
                     /locus_tag="ACG8Z3_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0822381.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(30807..31118)
                     /locus_tag="ACG8Z3_000027"
     CDS             complement(30807..31118)
                     /locus_tag="ACG8Z3_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822382.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            31299..32774
                     /locus_tag="ACG8Z3_000028"
     CDS             31299..32774
                     /locus_tag="ACG8Z3_000028"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0822383.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            32821..33405
                     /locus_tag="ACG8Z3_000029"
     CDS             32821..33405
                     /locus_tag="ACG8Z3_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0822384.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTIKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            33407..33826
                     /locus_tag="ACG8Z3_000030"
     CDS             33407..33826
                     /locus_tag="ACG8Z3_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0822385.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(34107..34391)
                     /locus_tag="ACG8Z3_000031"
     CDS             complement(34107..34391)
                     /locus_tag="ACG8Z3_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0822386.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(34743..35453)
                     /locus_tag="ACG8Z3_000032"
     CDS             complement(34743..35453)
                     /locus_tag="ACG8Z3_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0822387.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(35450..36922)
                     /locus_tag="ACG8Z3_000033"
     CDS             complement(35450..36922)
                     /locus_tag="ACG8Z3_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0822388.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            37060..37533
                     /locus_tag="ACG8Z3_000034"
     CDS             37060..37533
                     /locus_tag="ACG8Z3_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0822389.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(37686..38237)
                     /locus_tag="ACG8Z3_000035"
     CDS             complement(37686..38237)
                     /locus_tag="ACG8Z3_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0822390.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            38342..38872
                     /locus_tag="ACG8Z3_000036"
     CDS             38342..38872
                     /locus_tag="ACG8Z3_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0822391.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(39355..39798)
                     /locus_tag="ACG8Z3_000037"
     CDS             complement(39355..39798)
                     /locus_tag="ACG8Z3_000037"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0822392.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(39814..40974)
                     /locus_tag="ACG8Z3_000038"
     CDS             complement(39814..40974)
                     /locus_tag="ACG8Z3_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0822393.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            41481..43181
                     /locus_tag="ACG8Z3_000039"
     CDS             41481..43181
                     /locus_tag="ACG8Z3_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822394.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(43283..44116)
                     /locus_tag="ACG8Z3_000040"
     CDS             complement(43283..44116)
                     /locus_tag="ACG8Z3_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0822395.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(44420..46585)
                     /locus_tag="ACG8Z3_000041"
     CDS             complement(44420..46585)
                     /locus_tag="ACG8Z3_000041"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0822396.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            46845..49139
                     /locus_tag="ACG8Z3_000042"
     CDS             46845..49139
                     /locus_tag="ACG8Z3_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0822397.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            49132..49797
                     /gene="pgmB"
                     /locus_tag="ACG8Z3_000043"
     CDS             49132..49797
                     /gene="pgmB"
                     /locus_tag="ACG8Z3_000043"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0822398.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            49790..50797
                     /locus_tag="ACG8Z3_000044"
     CDS             49790..50797
                     /locus_tag="ACG8Z3_000044"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0822399.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            50819..51838
                     /locus_tag="ACG8Z3_000045"
     CDS             50819..51838
                     /locus_tag="ACG8Z3_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0822400.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(52119..53486)
                     /gene="nhaC"
                     /locus_tag="ACG8Z3_000046"
     CDS             complement(52119..53486)
                     /gene="nhaC"
                     /locus_tag="ACG8Z3_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0822401.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(53595..55016)
                     /gene="tyrP"
                     /locus_tag="ACG8Z3_000047"
     CDS             complement(53595..55016)
                     /gene="tyrP"
                     /locus_tag="ACG8Z3_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0822402.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(55259..57136)
                     /gene="tdc"
                     /locus_tag="ACG8Z3_000048"
     CDS             complement(55259..57136)
                     /gene="tdc"
                     /locus_tag="ACG8Z3_000048"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0822403.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(57427..58683)
                     /gene="tyrS"
                     /locus_tag="ACG8Z3_000049"
     CDS             complement(57427..58683)
                     /gene="tyrS"
                     /locus_tag="ACG8Z3_000049"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0822404.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(58795..59000)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            59263..60564
                     /locus_tag="ACG8Z3_000050"
     CDS             59263..60564
                     /locus_tag="ACG8Z3_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0822405.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(60633..61232)
                     /locus_tag="ACG8Z3_000051"
     CDS             complement(60633..61232)
                     /locus_tag="ACG8Z3_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0822406.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            61486..62367
                     /locus_tag="ACG8Z3_000052"
     CDS             61486..62367
                     /locus_tag="ACG8Z3_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0822407.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            62380..63990
                     /locus_tag="ACG8Z3_000053"
     CDS             62380..63990
                     /locus_tag="ACG8Z3_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822408.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(64067..64726)
                     /locus_tag="ACG8Z3_000054"
     CDS             complement(64067..64726)
                     /locus_tag="ACG8Z3_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0822409.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(65009..65674)
                     /locus_tag="ACG8Z3_000055"
     CDS             complement(65009..65674)
                     /locus_tag="ACG8Z3_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0822410.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(65821..66426)
                     /gene="dhaL"
                     /locus_tag="ACG8Z3_000056"
     CDS             complement(65821..66426)
                     /gene="dhaL"
                     /locus_tag="ACG8Z3_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0822411.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(66454..67464)
                     /gene="dhaK"
                     /locus_tag="ACG8Z3_000057"
     CDS             complement(66454..67464)
                     /gene="dhaK"
                     /locus_tag="ACG8Z3_000057"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0822412.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(67439..67834)
                     /gene="dhaM"
                     /locus_tag="ACG8Z3_000058"
     CDS             complement(67439..67834)
                     /gene="dhaM"
                     /locus_tag="ACG8Z3_000058"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0822413.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67988..68308
                     /locus_tag="ACG8Z3_000059"
     CDS             67988..68308
                     /locus_tag="ACG8Z3_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0822414.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(68420..69277)
                     /locus_tag="ACG8Z3_000060"
     CDS             complement(68420..69277)
                     /locus_tag="ACG8Z3_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0822415.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(69385..70689)
                     /locus_tag="ACG8Z3_000061"
     CDS             complement(69385..70689)
                     /locus_tag="ACG8Z3_000061"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0822416.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVSFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(70686..71195)
                     /locus_tag="ACG8Z3_000062"
     CDS             complement(70686..71195)
                     /locus_tag="ACG8Z3_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822417.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(71370..73505)
                     /locus_tag="ACG8Z3_000063"
     CDS             complement(71370..73505)
                     /locus_tag="ACG8Z3_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0822418.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(73588..75243)
                     /locus_tag="ACG8Z3_000064"
     CDS             complement(73588..75243)
                     /locus_tag="ACG8Z3_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0822419.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(75245..75559)
                     /locus_tag="ACG8Z3_000065"
     CDS             complement(75245..75559)
                     /locus_tag="ACG8Z3_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0822420.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            75729..75989
                     /locus_tag="ACG8Z3_000066"
     CDS             75729..75989
                     /locus_tag="ACG8Z3_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0822421.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            76003..76317
                     /locus_tag="ACG8Z3_000067"
     CDS             76003..76317
                     /locus_tag="ACG8Z3_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0822422.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(77047..77601)
                     /locus_tag="ACG8Z3_000068"
     CDS             complement(77047..77601)
                     /locus_tag="ACG8Z3_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0822423.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(77727..79656)
                     /locus_tag="ACG8Z3_000069"
                     /pseudo
     CDS             complement(77727..79656)
                     /locus_tag="ACG8Z3_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(79962..80678)
                     /locus_tag="ACG8Z3_000070"
     CDS             complement(79962..80678)
                     /locus_tag="ACG8Z3_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0822424.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(80679..82502)
                     /gene="glpO"
                     /locus_tag="ACG8Z3_000071"
     CDS             complement(80679..82502)
                     /gene="glpO"
                     /locus_tag="ACG8Z3_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0822425.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(82516..84012)
                     /gene="glpK"
                     /locus_tag="ACG8Z3_000072"
     CDS             complement(82516..84012)
                     /gene="glpK"
                     /locus_tag="ACG8Z3_000072"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0822426.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(84148..85608)
                     /locus_tag="ACG8Z3_000073"
     CDS             complement(84148..85608)
                     /locus_tag="ACG8Z3_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0822427.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(85598..86893)
                     /locus_tag="ACG8Z3_000074"
     CDS             complement(85598..86893)
                     /locus_tag="ACG8Z3_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0822428.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(87225..87902)
                     /locus_tag="ACG8Z3_000075"
     CDS             complement(87225..87902)
                     /locus_tag="ACG8Z3_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0822429.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87932..89023)
                     /locus_tag="ACG8Z3_000076"
     CDS             complement(87932..89023)
                     /locus_tag="ACG8Z3_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0822430.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(89124..89558)
                     /locus_tag="ACG8Z3_000077"
     CDS             complement(89124..89558)
                     /locus_tag="ACG8Z3_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0822431.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(90142..91599)
                     /locus_tag="ACG8Z3_000078"
     CDS             complement(90142..91599)
                     /locus_tag="ACG8Z3_000078"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0822432.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(91596..93491)
                     /locus_tag="ACG8Z3_000079"
     CDS             complement(91596..93491)
                     /locus_tag="ACG8Z3_000079"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0822433.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(93549..95324)
                     /locus_tag="ACG8Z3_000080"
     CDS             complement(93549..95324)
                     /locus_tag="ACG8Z3_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0822434.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(95374..96870)
                     /locus_tag="ACG8Z3_000081"
     CDS             complement(95374..96870)
                     /locus_tag="ACG8Z3_000081"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0822435.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLYYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(97064..98686)
                     /locus_tag="ACG8Z3_000082"
     CDS             complement(97064..98686)
                     /locus_tag="ACG8Z3_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0822436.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(99014..100327)
                     /locus_tag="ACG8Z3_000083"
     CDS             complement(99014..100327)
                     /locus_tag="ACG8Z3_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0822437.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(100572..100796)
                     /locus_tag="ACG8Z3_000084"
     CDS             complement(100572..100796)
                     /locus_tag="ACG8Z3_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822438.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100816..101238)
                     /locus_tag="ACG8Z3_000085"
     CDS             complement(100816..101238)
                     /locus_tag="ACG8Z3_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0822439.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(101380..102090)
                     /locus_tag="ACG8Z3_000086"
     CDS             complement(101380..102090)
                     /locus_tag="ACG8Z3_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0822440.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(102108..102605)
                     /locus_tag="ACG8Z3_000087"
     CDS             complement(102108..102605)
                     /locus_tag="ACG8Z3_000087"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0822441.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(102642..103094)
                     /locus_tag="ACG8Z3_000088"
     CDS             complement(102642..103094)
                     /locus_tag="ACG8Z3_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0822442.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(103229..103834)
                     /locus_tag="ACG8Z3_000089"
     CDS             complement(103229..103834)
                     /locus_tag="ACG8Z3_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0822443.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(104284..104904)
                     /locus_tag="ACG8Z3_000090"
     CDS             complement(104284..104904)
                     /locus_tag="ACG8Z3_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0822444.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104906..105775)
                     /locus_tag="ACG8Z3_000091"
     CDS             complement(104906..105775)
                     /locus_tag="ACG8Z3_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0822445.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105798..106205)
                     /locus_tag="ACG8Z3_000092"
     CDS             complement(105798..106205)
                     /locus_tag="ACG8Z3_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0822446.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(106224..107513)
                     /locus_tag="ACG8Z3_000093"
     CDS             complement(106224..107513)
                     /locus_tag="ACG8Z3_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0822447.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            107571..108910
                     /locus_tag="ACG8Z3_000094"
     CDS             join(107571..108012,108012..108910)
                     /locus_tag="ACG8Z3_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0822448.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT        32130 a        22556 c        19119 g        35107 t
ORIGIN      
        1 tacaaataaa ataaaggagt aatgatatga ttcccattta tatttgtgaa gacgatccaa
       61 aacaattaga agttttatca acggtgatca aaaatagaat catgattgaa aacttagaca
      121 gttatgtcca tatttctacc tctgatccgg aagaacttct caatgcagcc aagatcagaa
      181 cttcttcttt tggtatttac tttttagata tcgaactaga agacagtgaa atggatggtg
      241 tagcgatcgg taaattgatc cgtgaattag atcctttagg aaaaataatc tatgtgactt
      301 cccatacgga aatgtcgatg aaaatcttaa aaagcaacat cgaaccgact gattatatcg
      361 tcaaagaaga tctttatgat ttaaaagaaa atgtcgagca tatcctgagc aaaattttcg
      421 aagattttca aatgtcccaa atccaaaaag atatcttcaa aatagagttt aatgatgaga
      481 tcaaattttt accgatcaaa gacatccaat atttttctac ttcgctagga gcgccacata
      541 aactggaagt ccatttgact catgcccaat tacaattcta tgagaagatc aaagaaatcg
      601 agaaaatgca tcggaatttt atccgttgtc ataaatccta tgttatcaat acacaaaatg
      661 tgcggtcgat taataaaaaa accagagaag tcacgttggc aaacggcgag accatcccag
      721 cctctatccg tggattaaag aaattgattt cataaaaaga accgaatagt ttggaccgac
      781 taaatgacca gccgatccaa acccttcggt tcttttttgc ctatcagtgc aaaagcattt
      841 cctgtgcaat ttcacgtcca tccattgtat ctggataaaa tgttggccaa ttctccattt
      901 ctttcagcaa tacttcttga ctttcatttc ctgcatataa atgaaaatgt tcggagtcag
      961 ccggtgcaat cgtgtgatca ctgaattgaa tatatctaaa agcgccactt tccttatctt
     1021 ctgcttcgaa tagatacctt acgccacggt ttcctgcctc gtaggtcaag atttctttcc
     1081 ctacataacg ataatttgaa cgtttcgttg ttccattctt aaacgtaaat gccatctctt
     1141 tctcactgat cttgattgaa gcaatatctg tttgatagcc cttcgtataa tattctttat
     1201 attcttgtgc tgtcttatcc tgattcaatt tttctttata ttcaaaaaca ggatcaaaag
     1261 tcccatcttc tacatacgta tctactgttt gccaagttcc tgcccaatct gaaagagagc
     1321 gatcttgtac atcctcgtct tcaaaataac cgttgtagac gctcttttct tttttggggg
     1381 tcaaagtatc ttctagcggg ttttctgtct ctgtcgtttt tttcaacgct tcgaggtttg
     1441 cttccatgat agaaagatag ttttctccat tttctatttg ttcattcgtc aatccttcca
     1501 aaggattcaa tacttctaaa gatacacctg cttcgtttgc taacgttctg gcaatactat
     1561 ctttggcgtt ttcttcaaaa taaatatagt tgattccatg ttctttaaca aagtgtttca
     1621 gttcagcaat tctcgccgca gacggttctt cactagaaga aagaccagca atcgatactt
     1681 gattcaatcc atattctaac gctagatagt tgaatgcagc atgttgcgtg acaaaagatc
     1741 tttgttttgc ttgcttaaag gtttcttgat aaagctgatg gagtgcttga agtttctcgg
     1801 tataggctgc tgcatttttt gtccatactt cctgtttttc tgggtaagct tcaatcagct
     1861 gatcacgtat gtttgctacc tgcttgattg ctaaacttgg agctagccat acatgtgggt
     1921 ctagctcatg tgtatggcct tcttccccgt gttcatggtc atgatcatgt ccttcttcac
     1981 ttcccggaag taaaagcata ccttctgtcg cttttatcgt atggacattg ccttcttgta
     2041 atgttttttg gattgcgggg acccatgttt ccatgttttc atcattataa acaaagatat
     2101 ctgtatcttg gatctttgcc atgtctttag cagaaggttc ataatcgtgg gaatcagtcc
     2161 ctgctggaat caataactct acttctcctt catccccgac gattgctttt gtaaaatcat
     2221 acatgggata aaaggtcgtc atcacgctca atttttcttt actgtcctcg gctttcccac
     2281 ttgccccgca agcagttaac atacctgcta aaaccagcat ggccaatata cctatcaatc
     2341 gtttcattta tgacactcct cttctcatct tctttcattt gaatgttccg attcgctttt
     2401 cgcaaaccgt aatagttacg ttttagcaac aggatttaat ttatcatatg gttaaaagaa
     2461 ctgtcaagcg ttttcttatt ttaattgatg agaaagttta tatgaatgat tgacgatatt
     2521 tttgcaatca acctctatca ttttcctttt atacccgctt ttgtctataa tgaagttata
     2581 gataaaggag gaaaaagagt gaaaaaagaa acagatacaa cgaatcgttt gatccgcttt
     2641 ttaggaggaa aaacctctta ctatgtttta ggaatagtta tcttacttgc gctgatcatt
     2701 tattttttcc atcagatctc gtttatattc aagcctgtcg gaatcattat cgctactatc
     2761 ttgcccccat tattgtttgc actgatcttg tattatcttt tggaaccagt catcaaacgt
     2821 ttgagcaagc acctttcgag gaacttgtca gtcattatcg tttatgtgat tattttattt
     2881 ttattggggc ttggaagcat atggcttatt ccttttcttg aagaacaggc aaagacattg
     2941 atcagtagtt tgccagattt gtttcaagat tttcaagaga gtctccgaca atttttggag
     3001 aaaacacctt ttgccgattc atttaatcaa tttttcgctt ctattgacga cgtgacgtct
     3061 gatatagctg gatttattgg aaattattgg gaaagtggcg cacaacggat cagcaacatt
     3121 ttttcaactg ttacagctat ttttattact ttatttactg gtccaattgt cgcttttttc
     3181 cttttgagag atcctcaaaa attttatcag tcagttttag cgattgtccc accagcattt
     3241 cgtacagatt ttaaaaattt gacaaagatt gctaatcaac aattgggttc atttctaaag
     3301 gggcaaatca ttgcttcatt tatccttgga gcagtctact gggtttgttt cttactgatc
     3361 ggattagaat ttgctagtgt tttagctatt tctgctgggt tgttatgtat cattccatat
     3421 attgggccgt ttatcgcctt ctttccaggt ttgatcattg cattccaaga ctctactttt
     3481 atggctgtca aatttgtcat tgtctggttt gccgtacaac ttctccacgg tgacttggtg
     3541 attcctcgag taatgggtga tcgtttacag atccatccga ttacgatctt aatcgtttta
     3601 cttgtcatgg gtgatctaat gggaattgtg ggcgttattt ttggtattcc tatctatacg
     3661 ttagtcaaat tactcgttac ttttattttc cgtaaattca aacaacgcta caataaattt
     3721 tatggcgata aaggagagta tgaaaactct tcattttctg aagaagatta ttttaaataa
     3781 aacacaaaac gtcgatctta cgagaatagc tcgtaggaac gacgttttgt gtttttgttt
     3841 taatttttca gcaaagcaat tgcctggtat ggttttagct ccagactttg atgaagaaca
     3901 gtatcggaat aattatcgat aagaatattg gcttctgtga atccttctgg gatggcgatc
     3961 cttgtatctt tatcatagaa atttgttaat acaagcagct gctgtccttc aaattggcga
     4021 agaaagccat aaacttccgg atgatttgct tcataagcat gataatctcc ttgtgcaacg
     4081 attggatatg tttttctcag ccgaatcaat ttcttgtaat aggacaaaat agaatcttca
     4141 gaagatagtt ctttttctac attaatctct tctagcttgc ctccaacttt taaccaaggt
     4201 ttccctgtag taaatcctgc ttgttctgtg tctgaccatt gcatgggtgt tcgagagtta
     4261 tcgcgagatt ttgctttgat ccgacgaaac gcttcttttt ctgtgaatcc ttgttcaagg
     4321 agtaacttat aagcattcaa actttctacg tcaatatagt cggaaatcga tgaatagtct
     4381 gggtcgatca tgccgatttc ttctcccata taaatatagg gcgtcccacg gttcaaatgg
     4441 atcatggttg ctagcatctc tgctccttgt tttcgatact gatcgatagc tacaaatcga
     4501 ttgagcgcac gtggctgatc atgattatta aaaaatagag cattccagcc gttaccctca
     4561 ctcatctttt ctccccatgt atgaaatagt cgctttaatt ctgcaaaatc aaatggagga
     4621 gtagaccatt tattcccatt ttcataatcg actttcaaat gatggaaatt aaagaccatt
     4681 gataattctt ctctactagg ttctgtatac aagatacaat tttcgatcgt cgtagagctc
     4741 atttcaccga ctgtgatgat agtggaatcc gtcccaaacg ttgcttggtt caattctttt
     4801 agatacgtat gggtgattgg tttgtccgta tattcaggtt tgccatcatt ttctttatta
     4861 tttttcaatt gttcgtcttt accgataaca ttgataacat cgaaacggaa tccttttacc
     4921 cctttttcca tccaaaaacg aacaactttg aatagttcct ctcttactgc ttggttccgc
     4981 cagttcaagt cagcttgtgt ccgatcatat aagtgcaaat aatatttccc cgtatctcca
     5041 aatggcgacc aagcacttcc accaaatttt gatacccaat ccgttggctc ttctcttaaa
     5101 atgaagaagt cctgataatg ctggtcccct gctagtgctt ttttgaacca ttcatgttct
     5161 gtcgaaacgt gattcagcac catatcgagc atgatttcta tccggtattt ctttgcttcg
     5221 gctattagct gttcgaattc ctccatcgta ccaaagatag gatcgatggc tgtgtaatca
     5281 gagatatcat aaccgttgtc tttttgaggg cttggataaa tagggtttag ccaaatcatg
     5341 tcgatgccta gatcattcag atacgggagt ttttctttga ttccttttaa atctccaatt
     5401 ccatcaccat tactgtcttt gtaagatttg ggatagattt ggtaaatgac tttgtcttta
     5461 aatgtcatgg atatttacct ccattcaact gatttattac ctaccacaac ttatatatac
     5521 aagttatcat ttattattat aactgaaccg aaagcgtttg taaaatttat ctttatacat
     5581 tctaagaggt ctgttgccca ataaaaaaca agagatcaga catgtatttg tctatcctct
     5641 tgtttaaaat agcaaaatat tcttgtttct tctataaatg gtgttcaaaa gcttttatct
     5701 acggcaatca gtcttctcaa atgtaaaaca tgaggagcta attttatttg agtattcttt
     5761 ttgctcgatg caggacggct tttttacaac ctctgattca tggtgttcaa tcagctgtcc
     5821 ttcggtatta gctcaaaaaa cggcaaaata tgaaaaacta ttttgccgtt tgtttctcta
     5881 atactcaggt caaaccgctg atttcacaac ctctgttttt attcattcga tttcaacgca
     5941 tcaatcatat cgatatgctt taatttgaaa tgggtaacaa tcatcacaat aaccgtaaat
     6001 acgatagtta atcctgctga taagacataa gcccaccatg tgatcactaa tggaaagatg
     6061 acattctcca tagacgcttg ttgcaagatg aagtcagtca atatatatcc tacaccaaat
     6121 cctccgatga ttcctaaaag agtaaagatg atattttctc gaacaatgta catcgtgact
     6181 tctttatcaa aaaatccaag gacttttatt gtagataatt cgcgtacacg ctcggaaata
     6241 ttgatattag ttagattata caagacgata aaagccagca gtcctgataa tacgacaaag
     6301 attaagacaa ccgagtctaa gttcgccatt gattcttctt gcgtttcgat ttgtttggac
     6361 atgaaagacg tatttgtcac agcgtctgtg gcaagcagtt tctcggaaag catttcttct
     6421 tcttttgttg tcatcttatc tgtacgcagc aaaaatgcat tatccatcat cgattgatca
     6481 gtaactgaag cataatattc aggggacata taaataaagc tacctagata gtttttcacg
     6541 atttctttta cttcgacttt ccattctttt tgatcttcat cataaagcga aatatgatct
     6601 cctgtagata agtcaaatag ttctgctact ttttgcgtaa tagcgattcc tgttttaggc
     6661 aaggagcgtg ggttctcttc aagatcagtt aaatggataa aacgatccaa atcagctgtt
     6721 ttacttggta cgatcactgt cagactttgc gaagcatgtc ctttttttct caattctacg
     6781 gtttgagtat gaatagctag gatctcattt actttttctt gcttctgcag catatccgct
     6841 gctttttgac gactatcttc cgtggatgaa tccaatgtaa cgattgcttg atagtcaatg
     6901 atcggtccaa attgtttggc tgaaacagaa cttagagaat ctttcaatcc cacgcctgca
     6961 accatcagcc cggcacatcc agcaattccg ataatcgcca taagcattct tgacttatat
     7021 cggaagagat ttcggtaact gattttttga ttaaagctaa ggcgtgacca cagcggagtg
     7081 atgtattcaa gaaaaatgcg tttccctggc ttcggtgctt tcggttgaag caatgcagca
     7141 ggccgttctc ttaaatcttt gaataaaacg atcattgcag cgccaaatgc ggcaatgaag
     7201 aatgcggcag ctgcttgtaa aatcggcggc cagtcataaa aaataagcgc actcccaata
     7261 tcataccgct cgttagacag ctcaaaaatg atccttggca aaagattcgt tcctaatatc
     7321 gttcctaaaa caatacctat ccctgaggct agacttgcat agattgcata tttttttgcg
     7381 atttcaactt tcgtataacc taacgccttt aatgtcccga tttccctgcg gttttcttct
     7441 accatacgtg tcattgtcgt aaaggtgatc aatgcggcaa taaagaaaaa gaaaacagga
     7501 aaaacattgg caatcgcagc gatacgatca gacagatcac caaattcttg aaaaccgaca
     7561 ttttctgaac gttcattgta taggtatgat ggtttagtta aattttcaat tttttctgca
     7621 tttcgattca attcttcctc tgcagaggcc agttcacgtt ctttctcaac taattgcctt
     7681 tcttgttcag caagttgagc ttcttgttga gataatgttt gaacagcgat tgttcgttgc
     7741 gcttcaggaa gctgctggat agttgctgac tgttgcgcta actgtttttt agcatcttct
     7801 aaccgtgttc ttgcttgcgt tgtttgtgct ttgccatccg ctattttttg ctgtgccggt
     7861 tctaattctt cgttggccga ttttttcagc tcttctaagc gttcttcggg tcgatcttgt
     7921 aaatactttt ctgccttttc ttgattttgc tccattttat ctttgtaagc tttgctgtac
     7981 gtattcaaat tctttacgtt tgtaaaatca agatataaga cagaataaat atctgtttta
     8041 aaaacggatt caggtaaata gacaaaataa tcgacgctac catttccgac gttcgcgtat
     8101 cctctctcca aattgttgat gaacaatgga gaacggacaa agcccgtgat ggtatactct
     8161 ttttctttca attcatctga atcaatctga taaatatcac cgattttata cccttcatct
     8221 tccgcttttt catccaatac aagctggttt ggtttttccg ggagttttcc atcagttaat
     8281 tgtagcttgt tctgcttgtt atttttgtta taagaaaata tttgaatcac ttcgtttttg
     8341 ccagtattgg aataatagat ctgttttcct gtttctactt ttgcatctgg gatcttttct
     8401 gcttgagcaa tatctttttg tgtcaagcca ccagtagaaa cgatttgcaa atcagaaagt
     8461 cctgctgtac caacatagtg atccatcgtt ttttgcatga caggtccagc agttttgaca
     8521 cctacaaata aaagtgttcc tagcatgaca ataaggatga ttgcaatgaa tcgtccttta
     8581 gattgccgaa tttcgcgaaa acctgcttta agatatgttc ttgttttcat aaaatccccc
     8641 taccatacaa tctcatcaat ggatgaagga tgatcattta attcaacaga ccttacttta
     8701 gcatcattaa tgtgaatgac tctatcagcg atgggcgcta aagcagaatt atgtgtaatg
     8761 atcaaaacgg tattgccgtg ttgtctgcta gcgttttgca ataatttcaa tacttgcttg
     8821 cccgtttcaa aatccagcgc gcctgtcggt tcatcacata aaagaagttt gggattttta
     8881 gctagtgcac gagcaattga aaccctctgc tgttcgcctc ctgataattg ggacggaaag
     8941 ttattcaatc gatgagctag gcccacttgc ctcaatacct ctacaggatc aagcgcatca
     9001 ggagaaactt ccgttgccaa ttccacattt tcttttgctg ttagattagg aacgagattg
     9061 tagaattgga aaacgaaccc tacatctgtt cgtcggtaag cagtaagctg tttatctgaa
     9121 aattgcgcaa tatctgtgtc atcaataatg atctgacctt catcaggtgt atccatccca
     9181 cctaaaatat taagaatcgt tgactttcct gctccacttg gaccaagtat aatcactaat
     9241 tcgccttttt caattgaaaa agaaatatcg tcatttgctg taatggtcgt ttctcccatt
     9301 ttgtatcgtt tgtattcatt ttttacttcg acatacttca ctctgattcc tccattttca
     9361 cgatatttct cgctatatct atgcatcgaa tgcgttttct cacacttatt tattttacca
     9421 ctgaatttct ctagactaaa gaaaggaatc ctttaattta gttgacaaat aaacgattta
     9481 tgatttactt acataaaaaa cattgttatt tgaaagaagg aacatcatga atgatttaaa
     9541 ggaaaatatg atttttgatt tatcaaaaac tgcttttgtc gcgatcgaca tgcaaaaaga
     9601 gatcgtaaat aacggtacac tctctccaca cactgcagca tctattctga caaacaatga
     9661 tttgttagta agaacattga aacgtaccgc tgccttgaaa gttttagtga acgtagacat
     9721 ctccactttc ccttatttga gtcaacaaac tgatatgggt ggaatgactg cttctgtacc
     9781 tccagaattc actgatttac ttttgaagga ttctttgaaa gatacagata atatgttgac
     9841 tatcacaaaa tacaatccaa gtgctttttt tggtacttct cttgatcttc aacttcgccg
     9901 gcgtggaatc gaaactatca ttttaagcgg tgtagcgaca acaaacggtg tatatgctac
     9961 tgcattagat gcctttcaac acgggtacca tatcgtgctt gctgaagatg cttgcagtga
    10021 tcgtgacaaa gaatcccatc aattgttcat aaaaaagata tttccgaaaa ctgcacgagt
    10081 acgctcaacg aaacaaataa tcgaagctat ccaacaaagc aaataagtac tggcacacgt
    10141 gattttcatt tacttctttt tgatttcatg ggaaaataac cacaggattt agataggagt
    10201 atgaacatgg tgaaaaaaaa gatcacaatc aaagaagtcg ctaaactttc cggcgtttca
    10261 atcacaactg tctccttgat attaaatgga aatacggaga aattcagtcc gaaaactgtt
    10321 cagaaagttt tagctgccaa agaagaatta ggctatcagc ctgactattt tgctagacaa
    10381 atgattacca aggaaacgaa aacaattggt gtactcgttc ctgatatcac gaatccattc
    10441 ttcagtacac tgatgcgagg aattgaggat atcttgtata agcagaattt cgtcacgata
    10501 ttatgtaatg ctgattctga tcatcaaaaa gaaatcgaat atctagccga gctcacacgt
    10561 cgaggtgtgg atggattcat tatcgcgact tctgctgtct ctacagatgc aatcaacgaa
    10621 aatttgaaaa aacaaggccg gcccttcatt gttttggatc agaaaaaatc tgaaggattt
    10681 agtgatgccg tacgaacaga cgattttcgc ggtggttacc tagcgggcat gcacttgctt
    10741 agtctaggac atcaaacgat cgcccttgtc tatcctgaaa atccaccgga aaatgtccac
    10801 gctcggattg aggggtttaa atctgctctt gatgtttacc aaatccctca cgaccaactg
    10861 atcctcttac ctacacagtt ctcaaaacaa ggtggctacc agatcacagc agaacttctt
    10921 gattctgctg caactggtgt gtttgcttta aatgatgaat tagcttttgg attatatcgc
    10981 ggattagagg aagctggaaa aagcatccct gaagactaca gcattattgg ctatgataat
    11041 atagatatgt gcgagtatat caagccgaaa ctgacaacga tcgcacaacc aatcttcgaa
    11101 ttgggacaaa catctgccaa gttattgtta gatcggatcc aattcccaga aaaagaatgg
    11161 gaagaaaaaa gattacctgt acgattagaa aaaagatttt caactgcacc cttgaaatag
    11221 ctttcaaggt gtgctatatt tttatcgaac tattaaaacg ttttactaaa acgttttaaa
    11281 aaataatttt cccatcaaaa taaatgagat agttggaggt ttgtaaaatg aacacggtta
    11341 cagtaattgg aagtattaat atggacacta cccttcgttt aaccaacatg cccaagccag
    11401 gcgaaacgat gcatgcgcat gagatttttc acgctggcgg aggtaaaggt gccaaccaag
    11461 cagtggcagc caaacgttca ggcgcacgga catcttttat tggaggcgtt ggggctgaca
    11521 gcgaaggaca acagttatta gatctactga caaaagaaaa catcgataca tctgggatag
    11581 ctgaaatcca aggcgccaca accggacaag caatgattat ggtagacgca gcaggagaaa
    11641 atagtatctt gatccatgca ggggcaaata atgctttcca tgaacaagaa gtcctaaaaa
    11701 acaagcaact gattacaaac agtgacttta ttattgctca gtttgaaagt agcttagatg
    11761 cgacgatcct agctttttct attgcaaaag acgcaggaaa aacaacgatc ctgaatcctg
    11821 caccagctcg tgaaacgatt cctactgaac tacttgaaaa gacagatatc attataccaa
    11881 atgaaacaga aacagaaatc attacaggaa tcagagtgac agatcataac agtttggtcg
    11941 cagcagccga aaaactccat gaattaggta ttggaacagt aatcattaca ctaggcagtg
    12001 ccggcgcttt ctatcataca gaaaaagaac acggtatcgt ccctgctttt aaagtggatg
    12061 ccgttgacac aacagccgca ggtgatacat ttattggcgc attaagcagc acacttcagc
    12121 cagaccttag taacttgaaa gaagcgatcc tttatggaaa tctagcctct tctgttgctg
    12181 tgcagtctta tggtgcgcaa ccttctatcc cttaccgcga agctttagaa caatcagcag
    12241 tctaaaatta acaatgagag gaagaaacaa acatgaaaaa aggaaaagta atcaattcag
    12301 atatttcacg agtaatcgca caaatggggc actttgataa attaagtatc ggggatgcag
    12361 gcatgcctgt cccctttggt actgaaaaaa tagatttggc tgtctcaaac ggtcttccaa
    12421 gttttttatc tgttttagaa aacgttttag aagaacttga ggtacaatcg attgaattgg
    12481 cggaagaaat aaagacaatg aacccggaaa tattggaaaa aatcaaatta ttgttaccag
    12541 atacaccgat tagctttatc ccacatgaag aaatgaaaaa agagttgaat aactgtcatg
    12601 catttattcg tacaggcgaa atgacacctt acgccaatat tatattagtg agtggagtgg
    12661 tcttctaatg gaaacgacaa tgaatcctga acagatggta ctgtccgagc agattcatat
    12721 gctgattgaa cagttgggag gaaaagacaa tattgattct gtccttccat gtgcgactcg
    12781 gcttcgtatc aaattgaaaa aacagccagt agccaatgca gaaaaattaa aacagcaaat
    12841 cccttccatt aagtatttca ttttactcga tagatatgca gaaataatca tggatcaggg
    12901 attggctcct gcttacaagg aattgacgcg tcaaatcgat cattcagctc cgaaagaaaa
    12961 gctaaatccg atcgaaaaac tatttggttt gatatcggaa atctttacgc cgatcattcc
    13021 gctgtttgcc ggttctggta tattaaaagg tctggttgtt ttagcgacta cgattggctg
    13081 gcttagtgaa acaagcggaa cttatcatat tttgtctgct gcgtcgaatg ccgtctttta
    13141 ttttctgcca atctttcttg ctttttctgc tgccaaaacg tttaaagtga atggatatat
    13201 tgcggctgtc attgctgcag cactgattga accgaatctg acctctttac tttcagacag
    13261 tggcaaagca gctactgatt ttctggggat tcctgtagta ctgatgcagt atacgtctac
    13321 tgtcatgccg gcgattttag gtattttctt ttattctttt gttgaaaaat tcctcaaaaa
    13381 atatattcgc gaaaatatgc aattagtatt tgtaccttta ctttcattag ttatcacggt
    13441 tcctttgacg ttaatggtat tcgggcctgc gggtgtttac cttggcgaag gtttagctgc
    13501 cgcaattact tggatgatgg atattaacgg accgatcagt ggcgcaatca tcgctggtgg
    13561 ttggaatatt ttggtaatat ttggtatcca atgggctgta aaccctgtca tgatcagtaa
    13621 tatctctgct tatgggttcg accgaatcgt tccgttgaca ggagcagcca attttggaat
    13681 ggctggtgca gctttaggtg tatttctgcg aagtaaacgt tcaaaaaccc gttcgatctc
    13741 cggttctgct tttgcttcga ttttacttgc gggagtgacc gaaccaactg tttacggaat
    13801 tgcgatccca ttgaaaaaac catttgttgc tgcttgcatt ggtgcagctg ctggtggagc
    13861 tgtaatggga tttgcccaag tgaaagcgat cgcttttgta tttggtagtc tgaccacatt
    13921 gcctgcattc atttccggta cttttttctg gtacttagca ggactggctg ttagtctagt
    13981 cgtagcaatg atcactactc tcgttagcgg gtttgatgaa gacctaatgt cttatgaata
    14041 gttaatgaaa aagactgaag tatgagatgt ttatcacata tttcagtctt tttcttttta
    14101 ggatcattat ttttataaat ttttgtgata ctttccaaac tttggtataa tttacatatg
    14161 cataaccatt tttatcccca cttgtttatc cccaaacaaa ggtggtctat gcaataaaat
    14221 gaacgtcaaa gaaaaacgaa tgaatgctcg ttgcttattt acaaattgtt ttatttagaa
    14281 tacacagaca gatttatgaa aagaaaaagc ttttcgacac gattatctgt tccaaaaaat
    14341 gtctaatgaa tgctgaataa gacagaaaat caatgaaagg aaaaggacta gtttattatg
    14401 aaaaataaaa aaacgatacg tttgctagct gctacttggc tgattgctcc gtctgttttg
    14461 tcgaccaata ccgtatttgc cgtagataat caaatagctg atcaaacagt cacaacagat
    14521 acgactacat ccagctcagc tactagtgaa acaacggatt cttttacaga agacccaact
    14581 ggaacaacta actcaacaac aggatcgacc gagccaactg aaattgatcc ggctgatcca
    14641 tcagaaaatg aaaagccagc cgagacatat tcccttacaa ttgctccttc tttgattggt
    14701 aagatccaac tgactgcttc gacagcggat tcccctgaac agcaaccttt ggatattacg
    14761 gaaagtggaa cggtagaagg tttattagcg gaaacgaagg taacttacac tattacgcca
    14821 gctgaaggtg aacaaattgc ttcagtcgct gttaatggtg ccaaagaagt aaattatgat
    14881 gcaaagacct ttacagtagg tacgaacaat ttatctatta ctgcagaatt taaagctgga
    14941 ccggatactt caacagagaa accaactgat cctagtgaag aagtccctag tacaccagat
    15001 ccggaaacgc caaacaaacc agatgtgggt aataacaaca ataataatgg ttcaaataat
    15061 tcaaacaata ataacaataa ttcgaacagc gggaattctt cgaatcaaac aaacccatct
    15121 ggatcgaata acaatggaaa tactcagaga gaaccaaacc atccaaacaa tggttcatcg
    15181 aatggcggaa cgaacaatgc ttcatctcgt ccaaatacag caaatcaaag cggtatagag
    15241 aatccatctt ctgcttcttc tgattttgtt gtaaaatcac caattgatgc tgtattgcct
    15301 acaaatacaa cgaatgtcca acaagcaatc gttcgagaag cattcaagca tcttggaaaa
    15361 ccatatgtat ggggagcaaa aggtccaaat acttttgact gttctggttt gacttattac
    15421 gtatatatga aagcaaccgg tcattatatc ggtggctgga caggcgaaca gcaatatgca
    15481 ggtacgcaga ttccagtaag tcaggcccaa cctggcgacc tcgtcttctg gggaccttct
    15541 tccggtgtta ctcaccatgt aggtatctat attgggaacg gacaatttat ccatgcgcct
    15601 caaccaggag ataaagttcg agtaacttct atttcagatt tcacgcctga ttttgctgtc
    15661 cgagtaaact tagcaggttt acctgctgct tcaggttcgc tgacaggcgg ctcatctatt
    15721 ttagatggat tagatggctc gttccatttc agtcaaaacc aaacaactga tcagttcttg
    15781 aaaaagattg cagatgatgc acaggaaatc gggcaaaaag aaggaatcta cgcatcagtc
    15841 atgatggctc aagctatcct tgaaagtgga tctggaaaca gcttattatc aagcgaacca
    15901 aatcataacc tttttggtat caaaggatca tataaaggaa gcagcgtgac cttcaataca
    15961 cttgaacaag atagttccgg acaaagctac caaatccgcg cacaattccg taaatatcct
    16021 tcttacaagg aaagtttgga agactatgcc gacttgatca aaaatggact gactggcaat
    16081 cctgatttct acaaaccaac ttggaagtcg gaaacaaaag actacaaaga agcaaccaaa
    16141 tatttggaag gacgttatgc tacagatcgc caatattcac aaaaattgaa cgcgatcatc
    16201 gaagcgtacg acctgacaaa atatgatgaa ccgaaaaaag aagcagcaac aactgaagaa
    16261 gcaaacgctg aaatcctcaa tatgtctaag cgattcgatg tgccaatcaa atggacaaca
    16321 gatggcgtgc caatcacagg attagaattt tcaacgataa aacgcgtcaa caacgcttct
    16381 tccatcttcc gtgtcgcttc aatctgggat ttgtggaaca attttacggc aagacagata
    16441 ccagaaacaa ctactcgtac agttaaagct gcaactgttc ctgtactaac cttgttaagt
    16501 ttagacaaat cattctggct tgatctgcct ttaaagaaat agaataaatt aaaaagggga
    16561 tgtgacactg tcacactccc tttttaattt attctatttc tttatattta ttttgtattc
    16621 cgttacaaac tcgcgtactt ggttaggttc cagagtgaca tctccaaaac tttcaagtcg
    16681 ctccgctcct ggcagttctt gagtctcaaa tgtgataccg ccatgattta ctaatttatt
    16741 cccgcgcatt tccggtccat cttctccaaa atttgctgta aaaatcacga ttcctgaagc
    16801 atctgttttt acttggatcg atactttttc atctgggctt gtcaattccg cagcatttag
    16861 taaaccagat tgttttttca acaaaaatgg atgatcgatg ccgcccacta tcattgattg
    16921 gcgaaaatca ctggcaaaga cctcacctaa ttttttagga cgttggaagt caaatgctgt
    16981 gccacttact gaaactactt ctcctgtagg cgttgtatct ttattgagcg tagcaaataa
    17041 tcggctattc aaccaaagta tatgttcgtc gattgactgt gctggatcac cggttaaatt
    17101 gaaatagaca tgatttgtag gattgaatac tgttttgcgg tcactgatcc cttggaccgt
    17161 cactcgccat gtatcatcgt tcgtcagcgt ataccgtact tctacatcta aagttccagg
    17221 aaatccgttt tccatatctg ggctagtctg atagaaaatg accgatgctt cgttttcacc
    17281 attgatgact gtataatgcc actgcttggc ttcaaagcta ggtgcaccac catgtaaaca
    17341 atgaccagtg tccgggtctg tttttacttc gaacgtttca tcatctaatt tgaatgtgcc
    17401 attcgtaatc cgtccagcca tacgtccgat tgaagcaccg atataagggt ctttttctag
    17461 atattctttt gcttcatcaa aacctaagat caactctcgt ttttcattat cgattattgt
    17521 ttgaaaatga acaacccgtg ccccaagatc actgacagac aaagatgtcc cattttgatt
    17581 cgtgatcgta atcaagtgat agttttcgcc aaaattttgt tcattgattt ccatgttcct
    17641 tgcaccctct tttctttcat ttgcactgat tccagtatac atggaaaccg ttttcttaac
    17701 agcttattcc gtgagtaacg agaaaaaatt tactatattc taataaaaga ccgtgaaaaa
    17761 aaggttgtga caaaaatatt gtcacaacct ctttttccga acaaacggtg ttcaaaagct
    17821 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga ggagctattt ttgcttggct
    17881 gttcttatta cttgatgcag gacagctttt tcacaatctc tttttcaata cgattccagt
    17941 atataaccat gtcgtcagta catagtagat gaaatacaat acgaagaata tcgtaaacgg
    18001 aacccaaata tctttgtatg gattttgcat gaatagacca aacatttgta atccgaataa
    18061 gacatggact acacctaaga ttcctggtgc taagaataac acgcctactt ctctacggat
    18121 cgattgttta agcaacccgc gtcgtgttcc tatcttttca agcatttcat aacgagcaat
    18181 gtcactcttg cttcctgaca agatcttgaa catcaaacag ctagcaagca tggtcaagaa
    18241 agctaaacct aggaagaatc ccataaattc aaaaccagag aatagaccat tatacacttc
    18301 atacatttca tatttctggt tgctgtactc tactttttgc aaagatggat tgttcatttt
    18361 attttgagca acaagtgttt cgattggttt caggtttgct tggaagtctt ttacctgaac
    18421 tagctgtaaa gtggattgtg gcaagttcag ttgatcaaat tctgattgac tgactacctt
    18481 gatttctttt tcttgctgtt ccggcaattc atagcttcga agttcctctt gtacagtcag
    18541 ttcattcatt tttttagcag gaactttttc tttcgtgatc ttcggcggtg ttccatcttc
    18601 atgttttatc atgatcaatg gttgagccgc aaattcactt gcattatagt aaatcgttcc
    18661 cgcatcttct ttttgagtat aagaagcaga aagtgttggt gaaagttctt tgattttttg
    18721 ctgatcttct gcccgaggat tattcatcac tagatcatat ccagaattgt tttctgtcaa
    18781 gtctgcaatt tcatttttaa aacctaatcc taccgtcaaa gcgcccagtg ccaaggcaaa
    18841 cagcatagcg accattgaga gcatttgtgt atattcacga atacgaaagt ttaactgtga
    18901 caatgtgaaa ttgttcaatt ttttcaatga aatctgttct gatttcttta gtaatgataa
    18961 aaagaaaatg atcacagaat ggaaaatgaa ataagttcct aatacgatcg taactaaggc
    19021 tacccctata ccaaagactt gataggtccc cacgtgtttc atcatatagt aaccgcagcc
    19081 tagaaaagca attcccagta ctacttcaag gaaaaacaag aatttatgct gcttgattct
    19141 tgttggtgtc tgatccgcac gaatcaatgc taaaatcggt tttttgacaa tcgaaaaagc
    19201 attgacaaca gcagctaata aaaacaaaac agcaaaaaac aaaacagtga tcaacaaccc
    19261 gtgaatacta aaaggtgtaa aatgcgaaat attgagatct aactgatcca ctaataatcg
    19321 gtggacgata cttgtcaatc ctacaccgac agctgaaccc actaaagtag ctgcgatccc
    19381 tactaagaat gtttcagaaa aaatcatctg tgcgattttt ctgccttttg ctcccagcat
    19441 cataaacatt gcatagtctt tttgccgcat cgtcatcaaa aaggaatttg catataaaat
    19501 ataaacgaat gtgatgatcc ctagtaaaac agaaccgaat cggaaaatga taactgtagc
    19561 cgcaacgatc gtattacttt tcaaaaaagc ttcgttgctt gccattgatt caaacatgta
    19621 aaaaatagca gatgccataa ctaatcctga gaataacacc aagtagtctc ttaaacggct
    19681 cttgatccca gtaagtgata atttccaaag catagtcaat ctccccctac tgttccaagt
    19741 ttccaagaat agatagtatt tctcgataga aggaatcacg gctttgatct ccacgtttca
    19801 cttcttgatg gataccgcca tccttgatga ataaaatacg ttggcaataa cttgcagaaa
    19861 acggatcatg cgtcacaaga agaatcgata catgatcttt tgtattcaat tcgtccatcg
    19921 tatctaaaag atcccgggca cttttggaat ccagcgcacc tgtaggctca tcccctaata
    19981 gaatcgttgg ttgtgtgatc aatgcacgtg cggcggcgac ccgttgtttt tgtccaccgg
    20041 aaatctcaga aggatagctt tccaaaatat gagagatgga taaacgttct gcaatttttt
    20101 ggacacgttg cttgatttct tttggtctga cgccttgtaa agaaagagga acagcgatat
    20161 tttcttgtgc tgtcaagctt tctaataaat tgaaatcttg gaaaataaag ccaatctcat
    20221 tcgcacgaaa atctgctagt tgatttcctt ttaatgtggt cacatctttt tgattgattc
    20281 ggatatgccc atctgttggt ttatctaatg tggataaaat attcaaaagt gtcgacttac
    20341 cagaaccaga agcgcccatg atcccaataa attcgccttt ttctacatcg aagctgatgc
    20401 cgcttaatgc ttgcgtcttt ttctcattgt tttttccgta tacttttgtt acattctgta
    20461 cttcgactat tttgttcatt tctttcccta ccctttaatt agtttttcca gtgctgtttt
    20521 cggcacgtct tttttttcga cttcttcata cgaagtgaca cctttatttt tgttccaggt
    20581 gatttttaaa tatgctcctt tacgaagcgg tcgagtcaat tgtcccttga agtccagttc
    20641 tttttcctta ccctctttcg tatagccagg caggatatat ctatagttga ctgtttcttg
    20701 tccagagtct gattttgaaa ctgtttttgt accatctgtc gtaatttgta tatagtattc
    20761 ttctccaccc ataactacgt aatctgcgat tttcacccct gcaagtacca caacgatgaa
    20821 aacaagtaga ccgatcagaa gttttttcac tttttcccct tctctcttac tttatatact
    20881 gattatagaa aaaagcgaac aattctgcct tcgaatcaac tatcattctt ctttcaattt
    20941 tgtcattttc tttaaaacaa ctaaaaaaag agttcaagac aataatccgt cctaaactcc
    21001 ttcttcctat cttacaaaaa gtatgtcact tacggtcttt catgtcgcct gtattgataa
    21061 caggttgcgt tcctttgtct gtaatgattg agacaagtaa attattgatc aattggactt
    21121 tccgatcatc ggtgaaattg atttcttgtc catcttcgat ttgctctaag gccatttgag
    21181 tgattgtgac agctccttca acaatggttt gtcgagcaga caaaatggct tttgcttgtt
    21241 gacgttggag catggcacta gcgatttctg ttgcataggc taaatggttc aagcgtgttt
    21301 cgattacttc gacacctgct actgctaaac gctcttgcag ctctttttgc aactcgtcag
    21361 acactgccgt cgtgtttcct cgaagcgtca aatcattctc attgaatgta tcatacgggt
    21421 attggctagc gatatgccga atggctgtct cactttggat ttctacaaaa tcttgataat
    21481 acgctacatc gaataatgcc tttgctgtat caacgacttt aaagactact actgctgaga
    21541 tttcgatggg gtttccatcc aaatcattga cttttaaaac agaactattg aaattccgaa
    21601 ctttcaacga aacagtcatt ttttgtgcca aaggtatcgt aaggaaaaag ccattttctc
    21661 gaatcgtacc taaatattgt ccaaaaaaca aaattacttt tgcttgatta ggactgacta
    21721 cagttgccga acttaacaac aataacgtga tcaaccaaag aaaaatactt aaaaaaagtg
    21781 cccaaacttt cgcttgccac atacctaaat agaagaaaaa caatccaact aaagctaata
    21841 cgaccaatcc gacaatccct gcataaccat tgatataaaa tgctcttttt tctctcatgc
    21901 tacttcctgc gctcctttat ccttcaataa atatctaatg ttactttttt aatttttttt
    21961 tcgaatacgc gccattacac cttctagttc taactctttg tcttcagtga tgtaccaacc
    22021 gcttgcctgt gtccctccgg ctgcctgcgg taaataatcc aattttgttc gaacccagtg
    22081 cttttcttct gtaaaatagt cttctaccaa gacctctaga tccatccctg ctgaaagagg
    22141 caaatcttcc cgaacagatt taaagataaa tcgttccttc ttcccatcat acttcaatat
    22201 tcctgtatcg ataatcggta aagaataata atccagctct ttcttcaccg tcaataaatg
    22261 tttgaaaact tcatggtaca cttgctctaa ttggcgacct tctgaattgt tcgggtaatt
    22321 aaccatcact ttttctttgt ctggatcata gaaggtttgg cttagttgag tgatataagt
    22381 atgtaatctt tccatttctg tttttaactt ttctctttga ccttcttcta ccttcatata
    22441 ctctttcacc ctgttttctt tttagatatt ttctttctta ttttactata gttatgtgcg
    22501 ttaagcgatg aaaaacagca aagattggaa ggatctttgc tgttttttct taaaatatcc
    22561 agttgagtac aggatcaatc tgtttcattg tcctcttgat ccgtcgtaat attgatcgat
    22621 gtgtctttag cagtgatctt tagctttttc tgtccttgtt ctaacaaagt atttgttatt
    22681 ggctggtagt ctttttcaat ataagattca ccagacgttt ggatagtata gccactgttt
    22741 tccatctcat gcaaagataa attgccaccg tttaggatga cttttccttc tttgctgatc
    22801 aaactgtctt ctagatggaa gttgctgttt tcacctgtga cgatcaagtt atccacattg
    22861 gcctcatcta atcgaagtga gcctaattca tctttcgcag ttattgttcc tcctgtaaat
    22921 tcattgaacg atacccagcc atcatgtgtc ttagtattca cttttttgat tgatacttcg
    22981 ttgatatcaa ttgcactatt attcccttcg attgtcaatt cagataattc atgatctgca
    23041 gggacaacaa tagtgatttg tgcacttgtg tcttctaaac taatgattgc acctgttgat
    23101 tttttgactg cattcacagt caacacgcca tttttctcct ttatttctgg cttttctgtc
    23161 ttatcataag tcgtttttat ctcaaaatcc aatccgcgta tgaccgaaac tgtttgattt
    23221 tctgcatgga tgacgatttt gtttgcatcc ttgatttttt ttgtctcagt aaccatatcg
    23281 atcaaccgag gaccattatc ccaagtcaga ctcttttgag cgcctaatgt ataagttgca
    23341 cctgctagga ggccaccagt aagcatcaga ccaataccgg taatcaaaaa atgtttcaat
    23401 ctcaatttat gtgtcctcct tcaaacgccc gctgaatgca gatctctttt tgtaataaaa
    23461 cgtcgtccca tccatttaac aaagttcatc atgacataaa aaagcccttt tgttcctttc
    23521 agcaacagtg gcgaaataaa tactgcgctt cctgtggcga acagaccgac accgataaag
    23581 aataatccac tcagaatcga ttgtccaaac acactgattc ctgagatgat catgaatagg
    23641 ccaacaccta agatactgat acataaaaca tagatactaa aaatgagcgc tagtaaagca
    23701 accaatagtc ccccaataac caatataaac cccagtgcta gtggaatgag gacaggagaa
    23761 gcaaataaac cgataatgat taaccaaatc aggcgaattt gtctttttgg atttttcact
    23821 aactcgtttt ctgcttgttc ttcttctgaa aagtaactga tttttaattt taaggcaaac
    23881 tgctttggtg taccatattc atcaatgatc tgatctaatg tttttccaga atctaaaaaa
    23941 tattcctcat aatactggat caaatcaaaa cgatcgtctt caggaatttc ttgaagatga
    24001 tccacaactt cattcaaata ttcattcatt tgtgctctcc tccaataacc ggtctaaaga
    24061 atctcgaaaa acaatccatt catcaataat ttcttctaaa tgacgacttc ccattggtgt
    24121 caacgagtaa tatcgacggt ttctgccttc aaaaggttca tcataggtct caagatatcc
    24181 ttcttttttc aatcttctca aaaccggata tagtgtagat tcactgacag agagactttc
    24241 ttgtacgatt tttgttaagg catacccata catatcttcc ttgcttaatg ctgctagcac
    24301 agtaccatca agaagaacag ttggaatctg gatgtgcatt acgtcacctt ctattctcaa
    24361 gtgtaatatt ttatttcaat ctatattata caacatataa tatagtttca aaatcagttt
    24421 gtcaaataca aaaaaactga ctaaaagatt tgatcctttt tagtcagcaa aaaatggctt
    24481 ctctttgatt tttattataa cagtggcata atccaatttg tccacatcca gctcagtggc
    24541 atgataacga tcattgcagg tatcatgtca gctactggga acattttcac gttgatcatt
    24601 ctaaaaccgg tagcgaccat caagaatccg ccgcaagatt taaaatctaa gatcatgtct
    24661 ggtgtagtca atggataaat aaattttgcc aataagaaca aaatataaaa aataatgaat
    24721 tggggaattg ctatcacaga aacgacatac cctaaattac acgcaaaaat agctgctgtg
    24781 aaaaaatcaa ggacagattt agaaatcaaa atcgtactgt ctcctgtcat cccagaatct
    24841 aaacttccat agatccccgt accacttgca caaaatagaa caatgaccgt cactaatgtg
    24901 ctgataaatt catcgtgggt cataccaagc ttttcactag ggaagatttt agaaataggt
    24961 acttgcatca gcgcagcccc cttatttatc ccattaccta aatgaacaat caaaccaatc
    25021 gctgttccta tcactaaagc aaaaataaca gccggcatat ttttcatcgg cgcaatggaa
    25081 tagattccca tccccatcga acacacacca aaaaccatcg tcagctgtgc tttaaaatgt
    25141 tcacttaatt tatttcctac taatccgcca attagtccgc caaacaaaac ggaacctaca
    25201 ttgatcaaaa ctccagttgg catcaaaaaa ctcctctctt tttctacgaa gcgaaaaaaa
    25261 tcagaaaaat ttctgtaatt attgctttcg ctgcaagaaa gattgtaaca gacagagaag
    25321 attacaccaa ttcatttttt gtgttaaagt attctttatt ggaataagta ggaggaatat
    25381 gatggatatc cataaattcg aagtttttct tgatttagcc gaaacaatga atttcaccaa
    25441 aactgctgat cgacaattca ccacccaagg aaatatttct aaacagattc tttcgttaga
    25501 aacagaatta gacgtcaaat tattcgaacg cgcccataga aaaatcgaat tgaccgaagc
    25561 tggaagctta ttgctcccat atgtaaaaaa tgtcgtagaa caataccatg ctatgcaaga
    25621 agtgcttgaa acctatacga aagacaacaa tctttcatta aatatcttaa cgataccgac
    25681 aatgatcaat tataaaggtt tttccaaaat caccgagttt ttgaaacgcc atcctgaatt
    25741 tacagtccag ttgaaagaag tggaaagtgt cgaattaact tacaatgacg ccaatgaaca
    25801 tagtgacacg atccactttg ctagaagttt ccaggcccca ccaagttcga ttgagtggtt
    25861 gcctactgaa acagacgatt ttgtagcagt tctacctaaa aatcatcctt tagctagtaa
    25921 aaagaaattg gatctaagcg aactgaaaaa ggagaacttt ttgctccttg gccccactac
    25981 gaacttgtat caacctgtac tagatctgtg tcgcgaagct ggatttgaac caaaaatctc
    26041 ttataaagga gcaagaatcg acttgatcat caatatgatt gcaaatgaca tggggattgc
    26101 tattgttatg gaaaaaacag tcaagaatct attaaaagaa aatactgtga ttctccctat
    26161 ttctccaaca acagagagtt atcttgcatt tactcgtaaa gtcggtgagc attctttggc
    26221 ttcagataca ttttgggagt atttaaatca agaagaaaac taagccctac attttctgtc
    26281 catctatcca cccaaaaaaa gagagcgacg catgactttc gctctctttc ttgcatcatg
    26341 tccttgtttc aatgacacct ctaacatttc tcgtagttca ataagaaagc agcgattttt
    26401 atgccaaaag aaccgtaaga acatttccct aatcacaaaa aaacgactaa aaacccctaa
    26461 aaaccttcaa tagaattttt aggggtttta ttctttttcc tattgtttac ttaaccgtat
    26521 cattgctgtc gttcctactc cttcgatact gctataatca atacgtccat catgcagttc
    26581 tagaattttt ttaaccagtg aaagtcctag cccattccca gaaatttgac gacttgtatc
    26641 tccttgataa aatttttcaa acattcgatc tctggtctct tcattcattc ctacacctga
    26701 atcggtcact tccagtacaa tctctgtggc tgtctcaaat agtttgatat gaatctgtcc
    26761 attgatttga ttgtacttta tggcgttatc catgatattc agccacactt ggtacaaaaa
    26821 ctcttcattg cctgtgtaat ttactgcagc aagttcgata tccaattcta gctgttcttt
    26881 ctcccattta ggctgaaaaa aaacgattac ttctctgatt tgttcatcta atcgatactc
    26941 tttcttttcc agttggatac gctggttttc cagtttgttc aattttaaga tgttctctgt
    27001 taattgtgaa agctgcgtga tactttcgat aatccgatat aaaaatattt ggcgttcttc
    27061 gtcagagaga tttggggctt gtaacaattg aacatatcct tggattgttg ctagtggtgt
    27121 tttgaattca tgggatacac tggacacaaa gtcattcctc agtgtctcga tactattgag
    27181 ttcttggacc ataacgttga aatctttata cagttgctgt acttctgcta ctttctgctg
    27241 ttcatccatt cgaatcgaaa aatctcccgt tgctaccaga ctcatatttg tccctagcgc
    27301 actgattgga tgcaggatac gccgcccaac aaaaatcgat attcctgtac ccacgaccat
    27361 actgaacccc gcaaagacga ataacgggta taacgttggt tttccagccg attcagcagg
    27421 cgagatagaa tgtcgagaca ataagaaaat aaaaccgaga aaacacagca gtgtcactaa
    27481 gatcgaaatg aagacaatac tagcaaaata gatccataat tgtgaaaata gctttttttt
    27541 catctttaaa taaccgcctt atagcctagt ccccgtactg tgatgatttg aaaatcttcc
    27601 gtcttttcaa aacgggtcct taaacgtttg atatgtacat cgatcgttcg ttcttctgta
    27661 ttcgtatcta atccccaaat atcatccatc aactgttggc gagtgaagat cttgttggga
    27721 taagacagta atttatacag aagcagaaat tctttttgag gcagaatcaa cgaatgctga
    27781 tcttgtaaca ctgtataagc atctagctct aattttgttt gtccgattat caagctactg
    27841 ctgtaattga tctttgaacg tcgaagcaat gcttccacac gtaggatcat ctcattgaca
    27901 tcgatgggtt ttgtcatata atcatctgct cctaatttga acccgcgttt cttatcctca
    27961 aaatcggcct ttgccgtaat cattagaaca ggtgtgtcaa atctggtgct acgtaatgag
    28021 tctagcaagt catagccatc cattttcggc atcatgatat ctgtaatgat taattctata
    28081 cgtgtcgctt ctagtttttt cagcgcatcc tctccattca atgcggtgat gactgaaaag
    28141 cccgctcgtt ccaacacaga acggtaaagc aactgtaatt gcggatcatc ttctactact
    28201 aaaatacgat tcattgatct ccctctgttt tttgaaaaat ttctagattt tttcttgtag
    28261 ttcatcttca gttaacattt atttcgtata ctacgatcat tgttctgata tacttattag
    28321 tataccgaat atttcaatac gggagaaggg tgattatgct tcaattaaaa gatataaaaa
    28381 aatattataa agtcggcgaa accaccacca aagcattgga tggtgtgtct gtcgctttca
    28441 gacaaaaaga attcgtagcg attttaggtc ctagtggttc tggtaaaacg actatgctga
    28501 atgtgattgg tggactagat aattatgatt ccggtgatat ggtaatcaac ggaaagtcta
    28561 ctaaagactt taaggacagt gactgggatg cctaccgaaa caactctatc ggtttcattt
    28621 ttcaaagtta caacttgatt ggtcatctag gcattattga aaacgttgaa cttgggatga
    28681 cattaagtgg tgtttctaaa gacgaaaaac gaaaacgggc agaagatgca ttgcaccgag
    28741 tcggcttgac aaatcatatg cataaaaagc caaatcagtt atctggtgga caaatgcagc
    28801 gagtggccat cgctcgagca ctagccaatg acccagatat ccttttgtgc gatgaaccaa
    28861 ccggagcgtt agatacagaa acaagtatcc agatcatgga attgatccaa gagttatcga
    28921 aagaaaaatt agtgatcatg gtcacacata atcctgaact ggcccatcaa tacgcagatc
    28981 gaatcattga attttctgat ggtaagatct taacagattc ccatcctcac atcgaacgac
    29041 caaaagatga tcaatttaac ttacgcagaa caaagatgag tttttggaca gcgttaaagc
    29101 tttccttcaa caatatccga acaaaaaaag gacgtacttt cttgacctcc tttgcttcca
    29161 gtattgggat catcggaatc gcgatcgttc tgtccctttc ttctggattc caaaaacaaa
    29221 tcgataacac gcaagctgaa acgatggcga aatttccgat tactatttct aaggtaacga
    29281 ctaaccagac tcgtgacgat gccggattgg gtgcaagcaa agcggattat ccagacagca
    29341 aaacgattac tgcaaaagtc agcgaagaag ataaagcaca gcacacgaat aagattgatc
    29401 aaacatacgt cgattacgta acagacatcg atcctaactt aagtaataat atcggattta
    29461 cccgtactac aggaatcaat cttttacgtg atgtaaatgg caaagtacag cctgtcagct
    29521 tctcgaatca aaatcctgat acggaatctc tttctttatc tagtacgatg tcagcaatga
    29581 caggtgtggg agtgtcaagt ttccctacac agcttgacac tagcaaagaa aactttttaa
    29641 aagacaatta ttctttactt gccggaagtt atccagcctc agcaacagat gtcgtgctga
    29701 tcgtagacgg caataataat actaatatca acgcattgaa gaatcttgga tttgatgtga
    29761 aagaagacga aaaacttgat tttgatgata tcgttggtac aacattcaaa ttagtaaaca
    29821 acaatactta ctatacaaaa cttccaactg ggaattttat ccctaataca gattatgatg
    29881 cgatgtatca gaatgcttct gatgaattga agatctcggg gatcctacgc gtgaaatctt
    29941 cttctacaat gaatctctta tctccaggaa tcgcttatag cgatcaatta acgactcaaa
    30001 tcgtcaacga aaacaaagaa tcagaaatcg tcaaagctca aagagacagt ggtgtaaatg
    30061 tcttaactac tgaaaaagtt gacgaaaatg ccaaacaaac attgcttagt tatttaggtg
    30121 gagacagctt gccttcaagt atcatgattt atccgaacaa ttttgaagac aaagaaaaga
    30181 tcttggatta tctagatgat tacaataaag gcaaatcaga tgaagataaa atcatttata
    30241 ctgatttagc tggaacgatg actgaactga ctggcggatt gatggatgct atcacttatg
    30301 tccttattgc ttttgcaggg atctctttag taacaagtat gatcatgatc agtatcatta
    30361 cttatacatc tgtgattgaa cggacaaaag aaatcggtgt actaaaagct ttaggtgcac
    30421 gtaaaaaaga catcactcgc gtatttgacg cagaaacgtg tattttaggt atttcttctg
    30481 ggatattagg tgtgtttatt gcatggctag ctacgttccc tatcaatagc atcttataca
    30541 acatgacgga tctaaaaaat gttgctcaac taaatcctgt ccatgcgatt attctagtga
    30601 tcgtctctac tatattgacg atgcttggcg gtcatcttcc tgcacgaatg gcagcgaaaa
    30661 aagacgccgc tatcgctttg cgtgcagaat aaggttgtga caaccacatt tagatttgag
    30721 tcataagagc gctgaaatag tagaaaacag caagaaacga ggaagtaata tgttccttgt
    30781 ttcgcgctgt tttttttaat tattttttat aattctggta tacttttctt tcctttgtac
    30841 cacaactctt ctggcgtata cctgtctttt ccatttatga atactgttcc atctgcttca
    30901 atcgttactt cagtcccaat ccacatatta cgagaaagtg cgagaagatt ttttcttttt
    30961 cctcttcctt tttttccaaa ttgcaaggca tcggttatct cttctgtcca atgagatttc
    31021 acgtatttta cttctccgtt ttcaaatgta actttcaaaa tattcgctat attttccact
    31081 tttactgctt taggaaatga ctcctccatt tttttcactt tccttcctcc aacactaagt
    31141 atgcgctttc ttttagtgat aagtatatca tagtttattc tattatcaaa aaaacgcatc
    31201 cattccattg tagaatactt taccgaacaa gttgaattgt tttcctcttt ttcacctttt
    31261 actatagata ggtaaagaca actaaggagg aatctctcat gacagatcaa ccgtatgatt
    31321 taagaaaagt catcgaagaa ctaaaaacac ttccaggtca atatcacgaa acagatattg
    31381 aaatcgatcc tgaagcagaa ttatctggtg tttaccgtta tatcggtgct ggcggtacag
    31441 taaaacgtcc gactcaagaa ggaccagcta tgactttcaa caatatcaaa ggtttccccg
    31501 gcacacgtgt gaacatcgga acaatggcta gtcgtaaacg tgtaggtgaa attttgcacc
    31561 atgattatcg aacacttggt cgtttcttga aagacgctgt agaaaatcca atcaaacctg
    31621 tgaaagtaga caaagcagac gctccagcac aagaagtcgt tcatctagct accgatgcag
    31681 acttcgatat tcgcaacatt ttgcctgcac ctacaaatac tcctgaagat gctggtccat
    31741 acatcacaat gggtgtagtc ttaggatcag atccagaaaa aacaatgagc gatgtaacga
    31801 tccatcgtat ggttctagaa gataaagata cgatcggtat gtacatcatg cctggcggac
    31861 gccacattgg tcacttccaa aaacaattcg aagccttgaa taaaccaatg cctatcacta
    31921 tcaatattgg tttagatcca gcaattgcca ttggcacgac tttcgaacca cctaccactc
    31981 cacttggcta caatgaacta tggtgtgcag gagctttgcg taacgaacct attcaaatcg
    32041 ttgatggtat agcagtcgat gaagtgggta ttgctcgtgc tgaattcatc attgaagcag
    32101 aaatcatgcc aaatgaaaca atgcaagaag atatcaacac gaactcagga aaagcaatgc
    32161 cagaatttcc tggctatact ggggatgcta atccagctgt acaagtagtg aaagtcaaag
    32221 cagttactca ccgaaaagac aatccaatca tgcaaacaac gattggtccc agtgaagaac
    32281 acgtatctat ggccggtatt cctacagaag caagtattct tgccttggtc gataaagcta
    32341 ttccaggaaa agtagtaaat gtctacaatc ctcctgcagg tggtggtaaa ttgatgacca
    32401 tcatgcaaat ccgtaaagaa aacgaagcgg atgaaggtat tcagcgtcaa gcagctattc
    32461 ttgctttatc agccttcaaa gaattgaaga cagtcatttt agtcgatgaa gatgttgata
    32521 ttttcgacat gaacgatgta gtttggactt tcaacactcg tttccaagga gataaagata
    32581 tcatcgtgat cccaggaatg aggaaccatc cattggaccc atcagaatta ccagaatata
    32641 atcctggctc tattcgtttc cgtgggataa gtgcaaaaac tatctttgac ggtacagttc
    32701 ctttcgatat gaaagaacat tttgtacgtg caaaattcca agaagtagaa gattgggaaa
    32761 aatatttgaa ataatgaatg atctgcaact gttttgacat cataaaaagg agtgcggaaa
    32821 atggaacaat tggcacaacg gaagaaaaaa atcgtgattg gtgtaagtgg cgcttcaggc
    32881 accatttatg ccatcaacct agtgaagaaa ctaaaagaat atccgatgat agaaacacac
    32941 ggaatcatca gtccatgggc aaaacagaac ttaaagctgg aatccgactt atcactaact
    33001 gaattcgaaa gcctgtttga ttaccattat tcaaacaaag atttaggtgc tgctgtcgcc
    33061 agcggatcat ttttgaccga tggaatgatc atcgtaccgg caagtatgaa aacagtcgca
    33121 ggtatctctg ttggtttagg cgacaacctg attgcacgtg ctgctgatgt aacaataaag
    33181 gagcaacgaa agttagtcat tgttcctcgt gaaacgccat taaatgccat tcacttggaa
    33241 aacttgacta aattagcacg actaggtgtg caaatcatac caccaattcc tgctttttat
    33301 aatcagcctc aaacgatcca agatcttgtt aatcaccata caatgaaact tttggatcaa
    33361 tttgaaattg taaatgagca ttcaaaacgt tggcagggtg attaatatgg aaaaagaaac
    33421 gatagttgaa gtaaccaaac taggtttcac gatccaagct gctgttaaag taatcggtaa
    33481 agacttattg atcactttaa caggcggaga tacaccacat atcggaacag tcactacatt
    33541 ttctcgtgat acagagatgc aaactgtacg tttccctagc cacgatggtc ggttccataa
    33601 agacgatgtc ttagccgaaa aaatagctgt gatcatcaaa actgccgttc cgggaagttg
    33661 tacgatcact tccggtatcc atgttgacca tatctccaat gcacaaatca acgcctcttt
    33721 tccgatgtca gaagagttag gttacaagat tttagattgg ttggaaaaaa tagatttcca
    33781 tatcaaagaa ccagtttatt ataaagatgg tgaaaaacca ttatgaaaga agaggttgtg
    33841 aaatcagcgt tatgacctga gtatgagaga aaaaaatgga aaaatagctt ctcatatttt
    33901 gccgtttttg agctaatacc gagggacagc tgattgaacg ccgtttatca agaggttgtg
    33961 ataaagccgc ttcgtttcga gtagtaagaa aaattttgta aacatgggtt tccacatttt
    34021 tgcataaatt tgacttacta ccgagaaact ggcttttgaa caccgtttat caagaggttg
    34081 tgacacgtct tttgtcacaa cctcttttat ttttgcttca ctgtaactgc tacaatatca
    34141 ccgatttctt ttccgatgtt tttacgaata tcttttcgta gtccaaggat gtagcagact
    34201 gaaccatctg gatttttcac gcccatattc acgatactgc cttcatacgg cacattgtca
    34261 aaagttgctt ggactttaac tcttccagca ccgaattctt ttcttatatc atacggaaaa
    34321 ataatatatg ccccgccctt gccgacttcg gaagcataga tttttgattc aaaagaataa
    34381 atttccctca ttttatttct cctatttttt acatctagta tagcatagca aaaaaaagaa
    34441 ggaactgtga caaaagtctt gtcgcagttc cttttttcga ataaacggtg ttctcctgcc
    34501 aactcctcgg tcacaagtca caatagtcaa caaacatgag aagctgtttt ttgaaattgc
    34561 tccttgtgac tcagagttaa acggcaggct cacaacctct tccttaaacg gtgttcaaaa
    34621 gcttgcatct acggcactaa gcccaatcag atgaaaaata tggagtgcta tttttatctg
    34681 attgttctta ctgcttgatg cagagcagct ttttcacaac ctcctacatg gtgttcaaaa
    34741 tattattcgc cttttgcttc tttcactaat tttgcgaatg cttgttgttg gaattcagca
    34801 gctaaggctt tcgtacgttg ttcttgctct gctttgctca tttttggatt ttcagtcatt
    34861 gcttttacca taccgtcttg aacataagct tttgcttcac tttccatcgt actttgctgt
    34921 ttcttcacta attcagctaa cttggctgct tggtccgtac ttaattctac ccatgtctct
    34981 gggattttga atgtattcac tcggctgtca tattcgtgat tgttatcagc aatattgaac
    35041 cagaatttat acatgtcgtt tttgtattcc caataagtag ttttcgtcac tttttcagca
    35101 tcgccgtctg tcttttctac tttatttgtt tcgttatccg taccagttgc ttttggtttt
    35161 tcttgttttt catttgttcg gtaaagatac actttttctt ttccattccc taaagcttta
    35221 tacaaaagca tatctgctcc ttgggaatca gcactggaaa caattttttc tgtttttgtt
    35281 tccgttactt tttccattcc aaagtgattg gacaaattag cgacgatcaa acttagtgaa
    35341 gcgacgaaga ttagtccgaa aatcaaagat aaaaaggttt gccatgtttt tttagcgaac
    35401 acgttcgtta aagcaaaagc aagcaagctg acaatcaaaa ctccaataat catgcattat
    35461 cccccgcttt cattagaaca ggttcttttt tcttcaataa aaaggcaata ataaagccaa
    35521 gaatcccaaa gatgatcgct acgataaacg cggcatggta acctgacagt gtggcattga
    35581 ttgcctgatc tttataagct aatggcgtat tatccaatac agatttgctt gggatatttg
    35641 cttttgtcac attcgtcaac acactgatca aaatagctgt accaatcgag cttgcgattt
    35701 gacggaatgt attatttacc gctgtcccat gactgatcaa gttatttggc aatgcattca
    35761 taccagaagt cgttaccggc atcatgacca tggagatccc aaacatacgg atcgcataaa
    35821 ggatcacgat atataaaact ggagtcgttt ttgtcaagaa agcaaatggt gcagttgccg
    35881 ctgtcaaaat gaacatccct gtgatggcta aacgtttcgc gccgtattta tcgaaaatcg
    35941 cacccgtcac aggcatcata gcccccatga tcaatgcacc cggcaacaac atcaagccag
    36001 aatggaatgc tgattctcca cgaatatttt gaatatacaa tggtacgacc atttctgccc
    36061 ctaccatcgc catatttgtt acaccagcta aaacagctgc aatcgtgaat gtagatgatt
    36121 tgaatacacg aagttccaag aatggatgtt ccaaatgtaa ttgacgccat gagaacaagg
    36181 cgatgaaaat cacaccaaca actaagaaac caattacttt caagcttccc cagccgtcat
    36241 ctcctacact agaaaatccg taaagtaaac taccgaatcc aatcgttgat aaaatagcgg
    36301 ataaaatatc aatgctagga tttgacaatg gaataacact tttcattagg aagaatgcta
    36361 aaaccaagac aagaacaacg attggcagta ccataccaaa caagtcacgc caatggtagt
    36421 tatcaatgat ataaccagat aatgtagggc ctaaagcagg agcaagtcca ataacgatcc
    36481 ctgacatccc cattgctgat cctctttttt ctggcgggaa aatagatagc atgatatttt
    36541 gcaataatgg catcgatact cccacaccgc aagcttggat caaacgaccg attagtaatg
    36601 tactaaaatt aggtgctgta aagcaagtaa tcgttccgat caaaaagatc gtcatggcag
    36661 aaagatataa atttcttgaa ctgaatttat tgatcagcca tgctgttatt gggatcatga
    36721 tcccgttgac tagcaagaaa ccagtcgtta accattgcac atcagacgct gaaatatcga
    36781 aagctttcat caatgtagga aaagctgtcg tcaataaggt ctgattcaaa acggtacaaa
    36841 atgtaccaat taataagaca acaactaaaa gtgtccgatt atatggtttt ccgtaaatat
    36901 ctacgggttg gttgttactc atatttctcc ttctttctga attttttctg aaactactag
    36961 aatactttag tcctaaattc gctatttgtc aacctacttg acacataagt taatacggat
    37021 ataattaagg gaagcaagag gacaaaagga gtgacaaaaa tggttggggc ttcccttaaa
    37081 cgtttgttgg aaaatgatca attggttgtt ggcatcagtg aattaagtga tatcgtaggt
    37141 gtgtctccac gtcaattaag gtattgggaa caaaagggat tcattcgttc gatcgaagct
    37201 gatgcgaatt gtcctagaaa atatcgttta cctacagtca taaaagtaga actgatcaaa
    37261 aaatatttag atgacggatt tactttgtca aaagctgcag aaaaggcaga aatccgccaa
    37321 aagaaaatgc accatgtccg taaagttttt tcaaaagcaa tcaaagatat cgaattgatc
    37381 gacgaacgct atacgatttt acaaatcggt aacttcaaag aagagaatgc taagatgtac
    37441 atcattcatg acaacgagac agacgaatta acttacaagc tccttcctgt cgatgaaaaa
    37501 gctgactacg aacagttgtt aaaagaactc taaacgccac taatctccta agtaccaagc
    37561 tttttaataa gtagatgcta cataaatact tgaaaaaagg cctgtttgaa cataagaata
    37621 tccgaacggt gatctgcgtt tctttcatat agaaaggaat tctccatcac tgttcggata
    37681 tttttttatt tttttcggtt actttcttct aatacatctg ttagccaatt tgcatattct
    37741 gtttcccgat tgatcgcatg cgctaaaatc aggtagtgtc cgtaattttc atgtttttct
    37801 ttttcagtag gaaagatcgt tttttttcgc tctaatagat gaagtcgttt ttcttcatga
    37861 aggtcacgct gctgctgaat aatttctact aatgccggat cattcacgtc tttgacgaaa
    37921 tacaatttca gaacaaattc gtctcgatta accggtagtt cattggtggg tgtatgaatc
    37981 cactctgcca attgttcctt cccttcttct gttaattcat aaactttctt ttccaatttt
    38041 tcacctgtga tctctacttg atgctgaatg attccttgct cttccatttt tgcaagttca
    38101 ggataaatct gactatgttt tgcttgccaa aattctccta tttcattttt gaacgattga
    38161 ttcaattcat atcctgtcat cttcttctta cttaataacc ctaacaaaat atatcgaagt
    38221 gtattttttc ttgccatcag ttttccctcc ttgacaaatc tttaaatcca ttctatcata
    38281 aatgagtaat cgaaaacatg tagtttatta catgttttaa tttataagga gatgcttaaa
    38341 catgaaagaa tttaaagaat tggaagattt tattggaact cactttatct atacgtatga
    38401 caatggttgg gaatatgaat ggtatgccaa aaatgaccat actgttgatt accgtatcca
    38461 tggcggaatg gtcaaaggcc gttgggtaaa agaccaagaa gctgatatcg ttaaattgac
    38521 ggacggcgta ttcaaaatca cttggacaga gccaacaggg acagacgtgg cattggactt
    38581 tatgccaaat gaaaagaaac ttcacggaat gatctttttc cctaaatggg ttgaagaaca
    38641 tccagaaatc actgttactt tccaaaacga acatatcgca gaaatggaag cagctcgtga
    38701 aaaatatgaa acttatccaa aattacttgt tcctgaattt gctacgatca cttatatggg
    38761 tgatgcaggt atagacaatg aagatgttat tagcgaagaa ccatacgatg gaatgccaga
    38821 tgatatccgt agcggaaaat acttcgatga aaattataaa cgagtaaaat aaggttgtga
    38881 aagagaggtt gtgaaatcag cgttctgacc tgagtattag agaaagaaac ggaaaaatag
    38941 ttcctcatgt tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt
    39001 atcaagaggt tgtgaaatca gcgttatgct ttgagtaata agaacgctag ttagaaaata
    39061 gcttttcata ttttctaggc tagtgggctt attaccgaga agcagccgtt tgaacaccgt
    39121 ttatcaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca aatggaaaaa
    39181 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
    39241 tttatcaaag attgtaacaa ctgcatattc acacagttaa attagtgact tcaaaatgaa
    39301 ataatgctcc cagcaaaaaa ttaggctggg agcattattt cgttattctg ttcatcaaaa
    39361 atcagcgttt tgattccatt tcatcacaag ttcctcctca tttactgctt catctttttg
    39421 ctgtgtttga attgtaaaac cttgtgaaag atagaaatga atagctgatt gatttttttg
    39481 atagactgtc aaagtcagtg ttttattggt ttcttttatt tttttcatta gttgcgtacc
    39541 gattctttgt tgccggtatt cccttttcac aaagattcct gcgataaact gatcaatcac
    39601 cccagcaaaa ccaataattt cttgatctat ttctgctaag taaatcactg catctggtaa
    39661 agctttccta acagattcta cttgattctc ccaataagtt gatggaatga attcatgaga
    39721 atccaagtta gcatgcagcc aaatcgatag aacagattcc agttctttgg cagacaattt
    39781 tgttgttgtg cggatcattt tttctctcct tacttattca ttgattttaa aataaaaacc
    39841 atttttttga agctctttta caagttttcg cccctcaaaa aagtatatct tctcttgttt
    39901 tacttttcta ataccaaaag aaacagcaac tttaggatca tcagctgaaa cgattcgata
    39961 agttccacca ggcaattgat ccagcttgac tttcccatga tggtcgcttt gtactgaaaa
    40021 cggcacacca tttctagtaa gtgttttttt cttatgtttc aaaatgaatg aactaccatt
    40081 taccggtttc cctgacgaat cactaataat aaatgaaaaa tcaagcactt ttttacttaa
    40141 tctatactga tagattagtc gtccgataaa cactaacagt atgactacag ctattatcgt
    40201 tcctgcaaga ataaaccatt gccgcatggt tctttcctga tttccttttc cttcttcttt
    40261 ttttaccgat tcgttataag gaatccgatg tcctgtcact agcaaacgat gcgtgttgat
    40321 catatacggc gtacatgtaa gcaaggtaac taagtcttct ccttctgaaa tcgttagaac
    40381 agacgtgtca tctggcagta ccacttgtat atttttaact tgataagcca atttctcccc
    40441 caaaacagtc aaaataaacg tgtctccgtt ttctaaacga tctaattcgg taaataaccg
    40501 tcgattaggt aaaccgctgt gagcagagat cactgaatgc gtcgaatttc caccaagcgg
    40561 gaaagaggtt ccttgcaaga cgcctgctcc attttcaagg atcctaccat tgagccgatc
    40621 ataaagtgaa atatttaatt ggatcttagg tatattgata cttccgatca gccattcctc
    40681 taattggctg gatgttgtac tttcatttct gttcttttca ttgaaaggat cagtaccagg
    40741 attgaatcct tggtcagtta atcgttggtt tttctcctcc attttctttc gctgcacttc
    40801 aatttcagct gcggtccgtt tttgtacttg ttcgatacgt ttttgatcaa taaaactatt
    40861 taaagaatcc acataaaatg gatataaagc gatcagtaac cctacgacga tacatattaa
    40921 taaaaacaaa cgaaacaaaa gtcgacgaga ctgtttttgc ttactctgct tcacatgtgt
    40981 cctcccttgc aagttttttt gttctttctt ttattatatg ggttcaggaa aaaaatagaa
    41041 aatgaaagcc gatgaggttg tgaaaaagct gtcctgcatc aagtaataag tacagcccaa
    41101 aaaaatagct actcatattt tttgttgggt tgggcttatt accgcagatg caagcttttg
    41161 aacaccgttt aaggaagagg ttgtgaaaac tgcgttatgc tttgagtaat aagagcgcta
    41221 gtcagaaaat agcttctcat attttctagg ctagtgagct tattaccgag aagcagctga
    41281 ttgaacaccg tttaccgaga ggtcatgaaa aagctacttt gtttcgagta ttaagaaaaa
    41341 ttctgtttca aaacaaggag atgcaacaaa acttatgtcg catcctctct ccttagaact
    41401 ttcacttatg tttttcttgt aaaattcata aatattttca tgaattttct aatttcttgc
    41461 ctacctctcg cataggatat atgatgaatt tatctaaagt ttgcaaagga gttcgacaat
    41521 tgaaaaaata tggcccattt ttagcaattt ttttcattgc tttggcattt ctcatgcctc
    41581 atttattcac acataaacta attttgggtg ctgatgcccc attccattac aatcggttct
    41641 atgatgccgc gcaacaaatt aaatatgggg atttccatta ttttccttcc ctttatggat
    41701 tccagcaaag tggcaggatc gtgaatgccg tttacggccc atttttcgct tatttacaag
    41761 gattgatcgt gttgcttgca ggtagctggt ttcattatca gattctttca aatttcattc
    41821 tttatcttat ttcaggattt tctatgctga ttttactgag atatgtcaat gttcgaaatt
    41881 gggtaagtgt accagtagcc gttttattca tgagtacgta ttctattcaa tactggtcta
    41941 tcaaccaagg attcactagc tgggggactt gtttttatcc tttatgtatg atccctattg
    42001 ctgatttcgt ccttcataaa aagttccctg ttatcaaagt cgctgtctca gtgggactta
    42061 tgacacagat ccatttactg tcttcgatca tgttagcttt gatgtatatc cctttttatc
    42121 tttattactt ttttaaccag actgaaaaag gaaaagcact gcttcaactg attaagtcgg
    42181 tcttgcttta ttttgttctg acagcaaata tttgggcagg attagctatt gtttacagtg
    42241 ggaataagat tcttcctccc tttgttaaca gagaaatgag tcaaaaggcg atagatctgg
    42301 atggcagtta ttggctccat taccctaagg cctttcctat attgatatta tcaggttttt
    42361 tgctttttat tttctttcaa caaaagaaca ccttattcca aaaaatcgtg ttgtacacaa
    42421 gtcttgtttt cttttttctt tctactagct tgattccatg gacgacattg atcgaaaaaa
    42481 atgtgccttt tattagtttg atccagtttc catttcgatt tttcgtgcca tttacgctat
    42541 tgcttctact ttaccttgcc ctttcactga accagtggtc tgaacaaaag tggttccgtc
    42601 ttttaagtat atttggcatt cttatttttt cagcacagac gatccagaac ttataccagc
    42661 atctggaaaa gtgggaaaat gaaacatttg tcagtcgaca tacttatcta ttcgatacgc
    42721 cggaagaagc aaggaaaaca ttcttcagca aagatatgtc taccagcttg ttcgttttcc
    42781 aaaaaaccac acctgactat ctgccaatct ataaggagac aaaagacaat aaatacgacc
    42841 ggtataacga agaaatacta gaaaacgaaa caacttttat aaaaacacat ccaggcggtc
    42901 ttttgacaat caagtggaaa aacactgggg acaaaactgt tcatatccct gttatcgtct
    42961 acgatcggac tgtgctacag caaaatggaa aaacattgac tgattatgaa gtaacagata
    43021 tcgggacccc aatcgtgaag cagcaaaaag gaatcaacga gctcacgctt cattatcaaa
    43081 cgccaatcta tttctatttc attctttctc ttactttgat tggctggttt accttgctat
    43141 gtttatttat ttatcataga tacaagcttt tacgtgctta ggcaaaaggg ataaatacat
    43201 ccgatcaaaa aactctccaa acggtataat cgtttggaga gttttatttt aatcagtttt
    43261 tttatgttat atgttatatt ttttattcgt tcacatctaa ttcgatttcc accccgtagt
    43321 gatcactgat gattggacta tcgatcccat caaaaatccg ttgatatttt ttaacctgca
    43381 ttccttcagg gacgaaaata aaatctatac gcaatttttc tgtattttgt tcccaaccat
    43441 ctattttctt ttccacagta gctgcttttc ctttttcatt cgcgacaaca tacgaatctt
    43501 gaagaagaag gcaactttct cttacagtat aataaccttg agagtctact ggattattga
    43561 aatcacctag gaaaaaaagt ggtacgcgag tttccaaaaa gtagttttcc aagtttttcc
    43621 actcaaaata aaagccactt tttttatctg tccaccaaga aaagtggcag ctcgagacaa
    43681 aaatgtcctg atcatctatc tttgttttcc caatcaagat cttgcgtgtc tgataatctt
    43741 ctttcttttg aaattgggag acggtaagga cctgactttc caatgttttt ttcgtaagca
    43801 gtgcatttcc ttcttcatat ttatcataac cgatgtggct catttcccaa ctccaaaaat
    43861 actcttttcc gtgttccttt aaaagctgaa ctaaacggta ggcaaaatta tctttacgga
    43921 taggtacttg attctttacc ggacagaact tcattagctt cgcaggttct acttcttcag
    43981 attccagtaa ttggttgatt tcttgtaaag caatcacttc ataatcttct aacaggatct
    44041 tatctgctaa ttcttgcaat tttttctctg gttctggctc aagccagcta tgcgtgttga
    44101 gtgtcatgaa tttcattggt tttctccttg tcttcatttc gaaacaaaag ggttgtgaca
    44161 aaatttttgt cacaacctct ccagcaaata gtgttctcct gctaactcct cagtaataag
    44221 ctcaatggac caggagctat tttttgtttg gctgttttta tcacttggtg cagggcagct
    44281 ttttcacgac ctctccacta aacagtattc aaactgctgc ttctcggtaa taagcccaac
    44341 caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttatgacttg atgcaggaca
    44401 gctttttcac aacctctcct tacattttaa tggagccgat cactttacca gctgtcactg
    44461 tacctaattg cttgatcact acttcttcta ctttatcgtt tgtaaatacg atcatgatcg
    44521 ttggatcttt ttcttcgttt tggatcacat caagatccat ctcagctaat ttcgttcctg
    44581 ttcctacttc ttggccttct ttgacagcaa tctcaaaagc tggctgattc atttggactg
    44641 tatcgattcc catatggatc aaaacttcga taccatcttt cgtttccagc ccaattgcat
    44701 gtttgctagg gaaaatactt gtcacttttc cagcgatcgg cgcaacaact tctcttgatg
    44761 ttggaataac agcaaagccg tcacccatca ttttttgtga gaataccgga tcttttactt
    44821 cagataaagg aatcacttct ccagaagctg cggaaactag ttcttgttct atacctaagt
    44881 attgcttact acctgcttgt ccatttcctg tagaaaccat ttctggctct ggaatctctg
    44941 ctcctgattg cagcagatct tcgatatctg atttcaagac atctgctttt ggtccataaa
    45001 ctgcttggac tccattgtct ttgacgatca atcccatcgc ccctgctcgt ttccaggctt
    45061 cttcagtccc tactttttct cgatcattta cacttacgcg taaccgagtc atacaagcat
    45121 cgacatcttt gatattttct tttcctccca gcaaatgaat gatctggacc acttgctgat
    45181 ctgctgttcc tgttccgctg ttagatacaa cggtttcttc actgtcattg tcgtaatttc
    45241 cgttacgtcc cggtgtagca aagttgaatt ttttgatcaa gaaatttgct aagaaatacg
    45301 taacaacacc aaaagcgatc acacacaaca cgaagttgat aagatctcca cctagacccg
    45361 ctttgatagc taacggtgtc cgagtcagca attcgatatt cccaaatgaa tggacccgca
    45421 atggcaagat atctgccatc gcaaatgcag ccccttggat cactgaataa atgacatata
    45481 atggcacagc agcaaacata aacatgaact ccaatggttc tgttacacct gttagaaaga
    45541 cagccaacgc tgctgaaaaa tacattgatt tatatcttgc tttcttatca ggatcgacat
    45601 tacgatacat cgcaaaagcc attcccatca agatacctga agaaccaatc atttgtccta
    45661 ctttgaaacg agccggcgtc cagttatgca acacaaactc atattgcgac atatcccctg
    45721 cacctttaag attcactaaa tcagtggccc aagccaacca taacggatct tgcccaaata
    45781 cttgtgtccc cgcctgtgcg cctgataaaa tctcatacga tccgccaagc tgtgtatagt
    45841 tgataggaat cgtcagcata tgatgcaatc caaatggcaa tagtaaacgt tctaatgttc
    45901 caaataaaaa cggtgctaga atcggtgcgc tttcttgtga ttctgcaatc cataaaccaa
    45961 aattattgat tccagcctgt acgtttggcc acaccacagc aagaatcaat gccacgattg
    46021 ttgaccataa gataacaaca aacggaacaa agcgctttcc attgaaaaat gacaaagcat
    46081 ccggcaattt tctataatta tagtacttat tataagccat tgccccgaca aatccagcga
    46141 tgatccctac gaatactccc atattcaaag ctggtgcttc caatacactt gtaaaaaatc
    46201 ctttaaccat gatcttcgta ccgaacagag tgtgggtaaa tgcatcttca ttcgcaagca
    46261 tgtcacttgt gacaccaaag atagccccag tgatccgatt gattaaaata aatgagatcc
    46321 cagctgcaaa tgctccgcct gctctttctt ttgcccaact tcctcctatt gctaaagcaa
    46381 ataaaatatg aagattacca ataattgccc aaccaatatt ttcaattact ccgcccgttg
    46441 tgacaagcgg cgtccaatca ggattgatca agggaattga tttccctata ctgatcatca
    46501 acccagctgc cggcataact gctaccacaa ccattaaagc ctttccaaat ttttgccaaa
    46561 actcaaaact aaatatcttt ttcattccgt ttagctcctt tacgcaatcg gtttcgttaa
    46621 acgtattata gcgtgcgttt tcttttttgt aaatgcttta ttttttattt ttctgtctat
    46681 accattctat aaaacctata atacgtgtgt tcccttctat aattttctcc taaaaattta
    46741 tttttttaag cttttttcta tgaaatattt tcttttgtct attgacttgt gcaatcggtt
    46801 gcgtttatta tgaacgtata aaacagctca ttaggaggag aaaaatgaaa cagatcaaac
    46861 gactatttgc tattgatcca tggaaaatca gcacgacaca attagataaa aaaaatcttc
    46921 gactgcaaga atctttaaca agtattggaa acggttatat ggggatgcgc gggaactttg
    46981 aagaaactta ctcaggggac caccatcaag gtacttattt ggcgggtgtc tggtatcccg
    47041 ataagacacg tgtcggctgg tggaaaaatg gttatccaga atactttgga aaagtgatca
    47101 acgccttgaa ctttatcgcg atagatattt cgatcaacga tcagctgatc gacttggctt
    47161 ctttggaacc agaagacttt tactgggaat tagatatgaa aaatggtgta ttatctcgta
    47221 cattcacaat cactacttct actaataaag tacgcatttc cttcgaacgt tttctgagca
    47281 ttgtcaaaaa agaatcagct tatatctatt taactataga aatgcttgaa ggtagcggaa
    47341 cagtcaaact cgtatcaaaa ttggatggaa atgttcaaaa cgaagacagc aactatgatg
    47401 aacatttttg gaatgagatt gatcgtggaa ctatagaaga aatcggctat ttgacgacta
    47461 aaactattcc aaacaacttt gggattgaac aatttactgt tactgcagca atgcgccatt
    47521 tcgttaatgg aaaatcagtc atccctgact atcacgaaga tactttagca ttacatggtt
    47581 gcttgtctgc agaattatct caaggtgaga catggtcagt ggaaaaagaa attatcgttc
    47641 ttactagtag agatattcca gaagcgcagc aagcagaaaa agctgtctca gaattaacga
    47701 accagacaaa caattatatc caagcaaaag aagagcaaac agcggcttgg gcagaacgat
    47761 ggaagcttgc cgatgtggtc atcgaaggag acgaagaagc acaacaaggt attcgattca
    47821 atttgttcca actattctct acttactacg gcgaagatga acgattgaat atcggcccca
    47881 aaggattcac aggagaaaaa tacggcggag ccacttactg ggatacagaa gcgtatgctg
    47941 tccctcttta tcttgcttta gccaaacctg aagtaactaa gaatctgctg aaatatcggc
    48001 ataatcaact gccgcaagca atccacaacg ctcagcaaca aggattaaaa ggcgcgcttt
    48061 atcctatggt caccttcact ggtgtcgaat gccataacga gtgggaaatc acattcgagg
    48121 agattcaccg aaatggcgcg attgcctatg caatctacaa ttatacgaat tacaccggcg
    48181 atacggaata tttgaaaaat gaaggactgg aagttttagc agaaatcgca cgcttctggg
    48241 cagaccgtgt ccatttttca aaacgagcaa ataaatacat gatccacggt gtgaccggcc
    48301 caaacgaata tgagaacaat atcaataaca attggtatac aaatacgatc gcagcttggg
    48361 tcttgcgtta cacgcgagaa aattatttaa cttatcaaaa agaagctgct atcactatca
    48421 gcgaagaaga actagccaaa tggaaagata tcgcagagaa catgtacttc ccagttgacg
    48481 aagaattagg aatctttgtc cagcatgaca cattcttaga taaagatctc atgcctgtat
    48541 ccgatattcc cacaagcgat cttcctctga atcagcattg gtcttgggac aaaatcctta
    48601 gatcttgctt catcaagcag gcagatgtac tacaggggat ttatttcttc aatgatactt
    48661 ttacaaaaga agaaaagcaa aaaaactttg atttctatga accaatgact gtccatgagt
    48721 cttccctttc gccaagtatc cattcgattt tagcagcaga attaggaatg gaaagcaaag
    48781 ctgtagaaat gtaccagcga acagctcgct tggatttgga taattacaat aacgacacag
    48841 aagacggctt gcacatcact tccatgactg gtagctggtt gtcgattgtc caaggatttg
    48901 ctcaaatgaa aactgcccat gatcagttga gattcgcacc atttctgcca aaaacttgga
    48961 aagcttattc tttccatatc aattatcgcg gacgtttatt gttcatctca gtcacagatg
    49021 aacaagttac tttcgagtta ttagaaggtt ctcctctaga aatttacgtc tatgaacagt
    49081 cttattcttt agcggatcgt ctagaaataa cgctaagaaa ggaggaagca aatgtttaaa
    49141 ggggtattgt ttgatttaga cggtgtcatt accgatactg ctgaattcca ttatcgtgct
    49201 tggcgaaagc ttggtcagga aatcggcatt acgatcgacc acacattcaa cgaacagcta
    49261 aaaggcgtca gccgagaaga ctcactctct ttgcttttag cttatggcgg aaaagaacac
    49321 tctttttcaa aggaagaatt tgcagaatta gcgaaaagaa aaaacgatta ttatttagag
    49381 atgatccaga cgatcgaacc aaaagatgtc ttccccgggg tcgtgccttt actggattct
    49441 ttaaaagaag aaaaaatcaa gattgctctt gcttctgcaa gtaaaaacgg gccattccta
    49501 cttgaaaaaa tgggtcttac accttacttt gatgctattg ccgacccggc agaagcaaca
    49561 aacggaaaac ctgcgccaga tatcttcctc cttgcagcaa aagctgtcgg actaaaagca
    49621 gaagaatgta tcgggatcga agacgcgcaa gccggtatcc aagccatcct ctcaagtggc
    49681 gctcaaccaa ttggcgtggg acgtgctgaa gaccttggag aggagatccc gcttgtccct
    49741 gatacgactt ttcttacgat cgattacttg aaagaaaagt ggcatgacca tggataatcc
    49801 agagatctcc gtcgtaccag ttccagagac tgaaataaag aaaatcacat tgaagaacga
    49861 ctacttagaa gtcgttcttc tcaattacgg tgcccgcctg caccaaatct tcgcaccaga
    49921 caaagaagga aaatcagaaa atgttttatt gtcttacgat cgtttcgaag atgttctaac
    49981 agacaaaagc ttttttggag caactgtcgg acctgtagcc ggaaggatcc gggatgcttc
    50041 ttggggtgag catagattgg agaaaaactg cggatcacac catatccacg gtggcacgaa
    50101 aggctggtct ttccaatttt gggaagtaga agttttcaaa actccatcat caataggtgt
    50161 ggtcttttac ttaaaagatg aattttctac ctatccagga ccaatcactg ctacgatcac
    50221 ttaccgttta acaaaaaacg aattagagat gacaacctct gctagtagcc ttgttgaaac
    50281 catctgcaat ccgaccaacc atgcctactt caacctttca ggaaatggaa caagagacat
    50341 ctatgaacat cagttaacgg tctttttaga tggtgtgctt gaattagatc aagaaaaact
    50401 gcctactgga aattggacaa aaaaagaaaa actgccaatc gactttagaa aatccccaac
    50461 actgcaagag atccttgcat gttatccaga tggactagat gatgtatttc ttttgcatca
    50521 tcctaggctt tccaaaactt ccttacaatt attcgagaag cattctggtc gccaaatgac
    50581 tatagcaact tctaataaaa gtatggtatt attttctacg actggattcg aagctgattt
    50641 ctctgtcaat ggaaaacaga tgcattcaaa ctacggatta gcaatcgaac cgcaagaaat
    50701 ccctgatatc gttcatttcc ccaaatttgg atcgatcaac ctccatcctg gtcaagaacg
    50761 gatcagtcag acgatttatc ggttctctgc acaataacaa gagaaaggtg tttttatcat
    50821 ggctattacc gtaaaagatg tggcgaaaaa agccggtgtt gccacctcga ccgtctctcg
    50881 tgtcatcaac gatcatccta gtatttccga gacaacaaag aagaaagtcc gaaaaattat
    50941 ggatgaacta ggttatgttc ctaacatgac tgcccgcaat ctaggaaaac gcatttccag
    51001 tgccatcggc gtaatcttac cccctcttga ttcaaaagag cggatcggta acccatttta
    51061 tcttgagatc atggaagcaa tcaacgaaga agcgcggaat tatgatatga ctacagcaat
    51121 cgctactgct aaaaattttg aattgctgtt agagaacgtc cagcgtatgc atttacaaaa
    51181 acaagtagat ggttttattc tagtgtattc agatagtcaa gatactgtca ttgattattt
    51241 ataccaaaac cacatccctt tcactttgat cggacagcct tatcatcatg agaacgaaat
    51301 cgtttatgta gataatgata accagcttct aggtaaacaa gcaacagaat ttttgatcca
    51361 gaatggacat aagaatatcc tgtttgtgac aaacacgact catgaaagtt tgtatttcga
    51421 acgatatttc ggttatcaaa aagcaatgat gatggctggt cttcctgcgt tatccagtgt
    51481 agtactagag catccggagg attatctgtc ctttgagaaa acgttgaaag aaaacgaagc
    51541 cacagcattg gtggtcattg acgatatctt cgcccttcgc acgatgcagt tggcgcaaat
    51601 gtatggctat gtcattccag agacactctc cgtcattagc ttcaacaact cgatcttctc
    51661 aacattaaca cacccttacc ttacaagtat cgatatcgat atctccgaat taggacgtgt
    51721 cgctatgcaa aagctgcgcg aattgatcca tgaagaattg agcagtggcg tacgtttggt
    51781 catccctcat cgcttgatca aaagagagac agtcgttcct ttagaaaacc catactaaat
    51841 agctgactca ctaaatacgc tgaccattta tcctatggtc agctttttat gtaaaagaag
    51901 atactacttc taacacattg tttatctgta aaaaagagcg tgggacaaaa gtaaagaaca
    51961 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa
    52021 taagctgaaa ttcacaaaaa tttaaaaagc aattttcttg agtttcttct tatttcttgg
    52081 agttaaacgc ttctgtccca gcctcttttt tccttccttc aattttttac acgtcgacga
    52141 ttcaataaaa aaccgcctaa tattgtaaag acaggtgcaa gaatcgagaa aaaggcgaat
    52201 ggcaagtaat gcaacgcgct aactttcatc gttcccataa tgaatgtgcc gctgactccc
    52261 cacggtacga gagaattgac ggtcgcacct gaatccgcta gtgttcgtgt caaataggtt
    52321 tttgataagc caacgtcatc aaaagaacgc ttgaatgttt ctcctggaag aatgatggac
    52381 agatactgtt ctccaacgat cagattgatt ccgatacagc tcaaggcagt aaacccgact
    52441 aaacgagaag gtcggtccat cttttctttt aattcttgaa cgatggtatc aacgatcaga
    52501 tatttgatca gtaacccacc taatgccaat gcaagtaaaa tcaaactagc agaacctaac
    52561 atactcatga tcccgcctcg agaaagcagt tcatctagct ttggatcagg tgtatttgcg
    52621 acataaccat tcatcaagat gtctgctgct tttccgaccg tcaaactttt ctcatggata
    52681 aaagaaagaa cgagagcagc tgtcgatccc gctagtaatg tagggatagc cggaattttt
    52741 ttgattgccc ccccaaataa aataactagt ggaatcaatg tccaaggaga gatccaaaag
    52801 ctcgctttca tgttttggat catttcattg atactagcta gatcggctga atgactgtta
    52861 tgtcctaata tcgtataaaa aatcaacgaa aggacgaatg ctgggaaatc cgtataacgc
    52921 ataccagcga tatgttcgaa tagattaact cctccaatac ccgctgccaa gttggtcgta
    52981 tctgataaag gcgagcagtt attccctaaa aaggcaccgg aaacgactgc gcctgtagtc
    53041 attgcatcat tgaatccaag taaatgaccg atccccaaaa aagcaattcc aatcgtcgaa
    53101 atcgttgtaa atgaactgcc gacagtcact ccgacaattg cacaaacaag aaatgctgtc
    53161 ggtaaaaaga aacgcacaga aacaagttta aatccataaa ccataatcgt agggatggtt
    53221 ccagataaaa tccatgaagc aactaagaca ccaatcaaaa gaaaaatcag gattggaatg
    53281 atccctggag tgattccttt gatgatcccg tcatggatct catcccatgt gaacttttta
    53341 aatctgccat aaaacaacaa acacatgaaa gctgcaagga tcgggatatg tggcgagagc
    53401 tggaatccga tgatcagtac tcctaggagt gccaacaaca cgatcaatac agcgatactt
    53461 tcttttaaag aaatattttt attcatcgtc gatctcctat ttcaaatgaa aaggccctgc
    53521 catgaaaaaa ctattcactt ggcaggacct cctactaaat cagcgacaaa acgtcggctg
    53581 aaagacttat ttttttattt ttcttctaaa cttttttcta cctcaatccc ccaagaatct
    53641 ttcttgaagt gattgatgat caatggaata acgaacatga ccactgtgat agctgctact
    53701 aataatgcat cactcaatgt cgctgtacct aatgtagcaa atacgacaac gatgatagag
    53761 aagatcagca tgcagctgac tacccaagct aatccgtttc cgtttttacc aattcggtaa
    53821 ggacgttcga attctggatg tttcttacgc attttaagca atgaaacagc aatcaaaatg
    53881 tacacgatac aataaataac agttgtagca tttgtaagtg tcaagaatac agcacttacg
    53941 tcatccatta caccataaag caaagcaaat aatgaaataa cgattgattg tgttagaacg
    54001 acatttctag atacgccgta tttgttttct ttatggaatc cgaattttgg tggaaggaat
    54061 ccttcacgag ctacttggat gattgttttt gatggtccag taacccaagc tgataattgt
    54121 aaaagcacac caataaatac catgaagctg aagatatttc cgatcacttc tggtaagcct
    54181 aggatttgac agtcaatcaa gataggctgc gtgatatttg ctaattccat ttttccatct
    54241 ggtacgacat ttgctacaag catcgcattg atgacattca ataaaactaa tccgatcaaa
    54301 gcgataaata cgccttttgt ataatttttt gttgcgtctt tcaaacgagg catgtaaaca
    54361 gaactcattt cgatcccaac aaaaataaat gtgataccag ctaagtattt caatgaatca
    54421 atatgttcca agtcagggaa agcttttgac catgaaaagt cacctagata gccgtttgag
    54481 actagtccga ctttgaaggc tgccaaaaca cctaaaacga acatgatgac tactgggata
    54541 tataccccta gccatacacc gatgttcccg ccgacttttg ccatatcgaa tttcaagttc
    54601 aagatcgtga tgatccagta gataactaaa atacaaccta aaacgaacca gtgattattg
    54661 ccaagttcta cattaccaaa tgtgtttcct aacaatggtc ctaaagtaga agcgaccatt
    54721 accatacctg ggaacatttg cacccataat agccatgcaa cgacaaatcc ccatttgctg
    54781 cccagtgctg tttttaccca taactgagga ccgccttctt gaggaagcat cgtggaaagc
    54841 tcaccggaaa ccattgatat cggaccggca aaaaacaaaa ctgcaaaaat tgaataaaaa
    54901 atcaatgtcc aaccgactgc ggctaacgta ggaatactcc gaaccgttgc acacagtgcc
    54961 attgtcagac cgataaaggt cccaagaccc attttactta cttcagattt actcaccgtt
    55021 gacactctcc ttgatttttt ggataaaata acggtgtctt tgaagtagtt tcagcaataa
    55081 acgaaacaat tcagcgaata tttgagggga ttctttgagt ttattgcttg aagggtttct
    55141 tcaaagacaa cctcaaaaca aagggatcga agagcttagg tctccccttg ctcttcagat
    55201 ccaaacatgc gtcagaaaca gttagtattc aagctgtttt ctaacatgat taccttgctt
    55261 attttgcttc gcttgccaat aattggtctg cgtagatttt ttccaatttt tcttgaagag
    55321 ctgctttgat tttttcagca tattcttcaa agtgttcttc tttgttcatg tatggtgtca
    55381 ttacgctggc acgcaataca gttacttttc cggcacggtt ccattcttca tctgagaatc
    55441 ctagttggtt cacaaattgt aatgggctgt tgccatagtc tgggatagca aagtcagtat
    55501 gtgaagttaa gaactcatta ccgtaaatgc ttcctttcac gtatgaagaa taatcatata
    55561 cgtcatggtt caatttattc attgcaacta agtcatcgtt gcctttttct ttgaatacat
    55621 agtctaccat attgaaatct ggataagtta atggatgaac ttcgatttct ttgtctccta
    55681 ctttaaatga caagtcattc aagaagttgt agaaacgatg tgcaccttcg attgatgcac
    55741 ccattaattt accatatcct gtgacattca aaggtaagac atggtgtgct gcccaaacgc
    55801 tggctgctgt tgcccctgct tttgaacctt ccaagatata agccccaagt aatgctggga
    55861 tatctgcgcc tttttcaaat acatatgtcg caaagtaaga gataacgtct ctcatgcgga
    55921 tatctttgat gacgatacca ccggcagaat atggaacata gcccatttta tgtgggtcaa
    55981 tcgttacaga ttctgcttct tcgattgctt tatatgcact gtgtacttct tctaagatat
    56041 aatctttgtt ttctgtaaag acattgtatt tgtaatgaac atcttttaaa tcttcaaatg
    56101 gaatgaagtt gttgtcttcg tctaagaaaa tcgcacggcc gtatccgcca taagcagcat
    56161 ctacgtgtaa gtagaagtaa ataccgtctt tttctaatac gcgacgcaat gcaacgattt
    56221 tatcgatacc gtcgattgct ccttcttctg ttgatcctac aacaccgact acacctagga
    56281 ttggtgtttt ttcagctgct agaccacgaa cgattttttc aagttcgttg atgtccatgc
    56341 gatagttgtg gtcaactggc actggaatca cttggtctaa accgataccg atgatgtcgg
    56401 cagcttttag ccatgaatag tgtttagttt gtggaaccaa ccattttcct aatttttcta
    56461 aatgttttcc gctacgtgct gagtgagctt tgatttcgtc gattttttct gggacgctgt
    56521 ctaacagatt catgatttct tctgttgaca agttcattaa ttcccaatcg ctttttccag
    56581 caactagttc aggtgtcact tctttcattg caagcggcaa tgatttgatg ttgcgggcat
    56641 accaaagtcc ttctaagttt gctaaagaac catcagcaac gatgtggccc cagccatctt
    56701 tatagctcat taattttgca aattccattc ctacttcttc ttccatttgg cttgttgcag
    56761 gagaagattc ataggcaacg ttattaccat tccatagcat agcaaaattg tacgctagta
    56821 aagatggcat caatgtttca gagttcatat gaccccagta acgtccggca ttatgccaag
    56881 gaacggaatg ggtacgcatc cgcgctgaaa tttctgatag aacatccttt gttttattca
    56941 cagtgtgttc aaaactagca ctgctttttt cttctggcgt aataataggc atgtcttgag
    57001 gcatgtagtt ttgacgccag cctaaatgtt catccaccaa ttcattcaac aaagctttat
    57061 agatttggcc gttctcagct ttgtccccaa tgaatagggc atttaggttt agatctttcg
    57121 acaatgattc actcatagtg aaaccctcct aaaaaaatat ttaaattttg tcgagcccta
    57181 cattacacct gttcttctat tctgtaaact aagttttggc ctgattttta atcatatttt
    57241 aatatgtatc aattatccga attgttcaca cttttgaaat aattaaaaaa aacgagggaa
    57301 tttacaaatg taaactccct cgcaaagatt gtattaacga caaccagcgt tctattttag
    57361 gatgggtttc aaaattggaa tgttgtaata gtatttccct ttttcaaggt aaaaacttac
    57421 taaattctaa tcaattactt tggccaaaaa gtaatttttc ttaccttttc gaataaccac
    57481 aaacttacca tcgaattcat cggatggatt tatgacaaaa tttaaatcgg taacgcggtc
    57541 accgttaata cttatagctc cgtttgaaac atcttcacgt gcttgacgtt tagatggttc
    57601 aatttttgtg gaaacaagca gttccacgat attttcaggt gtgcttgaaa tttcaacgtt
    57661 cggcatttta ccgaatcctt gcgcgatttc ttccgcattt agttctttaa tatttccaga
    57721 gaacaatgct tgcgttattt tttgcgcttc tttcaagtct tcttcactat gcacaaatct
    57781 tgtaacttct tctgccaaac ggcgttgtgc ttcacgtttc tcaggttctg tctctacttt
    57841 tttcgctaag tcttcgattt cttcttgaga caagaaagta aagaatttca agtatttcac
    57901 tacatcgcga tcatcttgat tcaaccagaa ttggtagaac tcaaatggtg aagttttctt
    57961 cggatcaagc caaacggcac cgcctgcagt ttttccgaat ttcgttccgt cagccttcag
    58021 catcaatggg atcgttaaac cgaacgcttc tgcttctggt ccttctaatt ttcggattag
    58081 gtctaatcca gctgtgatat ttccccattg gtctgctcca ccgatttgca attgcacatc
    58141 gtatgtttta tgcagatgca aaaagtcgat agattgcagg atttggtaag taaactctgt
    58201 aaatgagatt cctacttcca aacggctggc aacgatatct tttgctaaca tcgtgttgat
    58261 gttgaagttt ttaccgtagt ctctcaaaaa ttcaagtaaa gagatttttg acaaccagtc
    58321 gtagttgttc acaaatgtga tatttgcatc tttaccaaac aattttctca tttgattgga
    58381 aagtgcgtcc acgttatgtt gcacttgttc catcgtttgc aacacacgtt ctgttttgcg
    58441 gccgcttggg tcgccgatcg ttcctgttcc gccgccaatc agaatatatg gatgatggcc
    58501 tgccaattgg aatcttttca tcatcataaa tggaatcaaa tgtccgatat gcatcgaatc
    58561 accagttgga tcaacgccgc aataaagtga aatgctcttt tcttcaacga gctcgcgaag
    58621 tctttcttcg tttgtttgct gattaatggc gtcgcgccaa gttaattcat caataatatt
    58681 cattttactg acctccgtgt agttttttta tgaaagcagg aacctgctag catatgtaat
    58741 taattatttc ttcaggatag gataggaaat aaaaaaagtc ccttggcata taagccaagg
    58801 gacgaaatct cgcggtacca cccaatttgt atgtctagat atccaacata cctctcgaac
    58861 atttaacgct gtcgcgtggc aatcactgcc aaagactcct gaaatgtaat tcgctttaaa
    58921 gcatgtactg gtttgcagcg accaccagct ttctgaaaca ttggctttaa caactacttc
    58981 gtttcgatct tcgtcttgtt attattaata tgtgtctatc atagagcgca actttttctt
    59041 tgtcaacaga aaaaggatat tttttttaaa ttcctcattt tttctttttt gaatccgctc
    59101 taattccgaa cattttattt tcaattattg ttaaaagaaa taactttttc tgtatttttt
    59161 ctgcaattta tcgagaagtc actttacttt gcctcttatt ctttcttcgt tacaataagg
    59221 aatgggacaa gtaaaaaaat gaaaaagcaa gggacgaggt atatggaaaa aaagcaaaaa
    59281 attattttag gagccacttc tgctttaact atcgttattt taattctaag tttgtttttt
    59341 gtcatccgat catctacaaa aaaagaacca atcattcctg aaaaaaaaat aacagaaaaa
    59401 gcaaaagaac cgacagatga taagcaacaa ctagttactc aagcaaaaaa tctggctcaa
    59461 agctacgatt atgaccaagc gattgcgtta ttggaaaaag atagtaagaa cggatctact
    59521 gaaacacaac agcttcttca aacactaaaa aaggagaaag aacaactgat cgactggtct
    59581 gatcctactc agatctctca tgtctttttc cacagcttga tcgtggaccc gcaaaaagcc
    59641 ttccactctc aacaggcaca aggatacaaa gattatatgg tgactgttga agaattcaac
    59701 cgttcgatcg aacaacttta tcaaaatgat tttgttttag ttaatttgaa tgatttgatc
    59761 caaaaagacg aacaaggaaa tctttccttt aacgggcttg cgctacctga aggaaaaaaa
    59821 ccattggttc tttcacaaga tgatgtcagc tattatgaat atatggataa ttccggtttt
    59881 cctagccgat tggtcgtatc aaataacaat gaaatcaaaa atgtctataa agagaaagat
    59941 aaagaagaaa tcggtgactt tgacatggtt cctctgatcg atgcctttgt caagaaacat
    60001 cctgattttt cttatcgagg cgcaaaaggt accttggccc tgacaggtta caacggagtt
    60061 ttaggttatc gaacatcaaa atctcaatat ggagacaatg aaaaaacaaa aaaagagatt
    60121 aaagaagcta aaaaagtggc ggaacagttg aagaaagacg gctggagctt tgcttcccat
    60181 acatggggtc acttgaatat gactcaagct tcactagaag atgtaaaaaa agacaatgaa
    60241 ttgtggcagc aagaagttgc acctatccta ggaaaaacaa atattctgat ttatccattt
    60301 ggtgcagaca tcagcgactg gcagccttat tcagcagaaa atcaaaaatt tgcttacttg
    60361 aagagtcaag gtttcgatat tttctgtaat gtcgatgctt ctacaccagc ttggggacaa
    60421 ttaggtacgg atttctatcg aaatgctcgg atcaatatcg atggcattcg ttttcaatct
    60481 gatttagacg gaaaaaatcc tattcttgat ccattcatca atgtccatga tgtctatgac
    60541 aaagaagcac gcgaatctgc ttgattttac gtcatttatg aaccaaaaaa gccgaactga
    60601 cattttatcg tcagtttcgg cttttttatg ctttatttct tcttcggtgc gattcccttg
    60661 atcaggactt ctgtccattc tgctgtatat ggaagcagtt ttgcgtatat ctccggctta
    60721 gaaattcctt ctaccattgc ttgaaaatag aggatcaaga actcatccga atatttcaaa
    60781 tccacttttc cttctttccg ccctcgatgg aaaagatcaa gcatgatact gaaactttct
    60841 tgcgtatacg catccatcgc gtcagaaaat tctttttttt ctttttttgt atagtatgtc
    60901 atcaaatcga tataaaattg tttgttcact cgattgagat aacggatttt gttttcactc
    60961 atggcaatca gtgtttcttc aaatggaaga ttttccgcaa tcaacgcttt cgcttcttgc
    61021 cctaaatcat tcataaatgc aaaaaatact tcataaatca gcccttcctt gctgccaaaa
    61081 tatttaaaaa tcgtcgcttt acttacctga gcgttctcag ctaccttatc cattgtcact
    61141 ccagcggttc ctatgtctgt gttcatcaag tcaaaagctg cttgtaaaat ctctttcttt
    61201 ttctcttgtg ctctcttttc aaaaccgttc atttgtagag cctcccgtaa ttttaaactt
    61261 ttataatata tatagttcaa atttattctg aggtttatta taactatcta ttgagaaagt
    61321 atcaagctag caattccttg tttatgaatt cattgacaga aatttataaa aagaatatta
    61381 tgtactaaaa taataaaaaa agttttataa aaaggtataa aaccttttta ttaaactatt
    61441 taatcttgtt tagtatatat ttttagaaaa aggagtgggg tttttatggc agaaatcgtc
    61501 aaagtcacac atttgaaaaa gagctttggt cgtttcgaag ctttaaaaga tgtctctttc
    61561 accgtacatt caggtgaagt cgttggattt atcggaccaa atggttctgg aaaatccacg
    61621 acgattcgga cacttttagg gattataaaa aaagacggcg gaacggcaac gatttttggg
    61681 caagatgtat gggaagatag tttatctatc catgaacgtc tttcttatgt tccaggcgat
    61741 atctctttgt ggggaaattt gactggagga gcgatcatcg atctcttcat gaagcttcat
    61801 ggaaaaggga gtgctcaaaa aaaagatgaa ctgattcaac gtttctctct tgatcctaag
    61861 aaaaaagcaa aaaactattc aaaaggaaat cgccaaaaag tgggcttgat tgctgctttg
    61921 gctgttgatt cggatctcta tttatttgat gagcctactt ctggtttaga tcctttgatg
    61981 gaaacgattt tccaagaaga agttgaaaaa ttgaaagaac agggcaaatc catcctactt
    62041 tcttctcata ttctcagtga agtcgagcga ctagcggata aagtcgtgat catacgcgaa
    62101 ggaacactcg atgaagaagg aacactcgat gaattgcgtc atttgactcg ttcgaccatc
    62161 actatagaaa cagaagcaga gaatagtcac ttagcacaat tgaacggtgt ccatgacttt
    62221 gtgcaaaaag gtcacctcgc tcaatttgct gctgataacg aagctttcga tgtgatttta
    62281 gctgaagcgt ccaaattagg tgtgaaaaaa tttgaatcta ttccaccgac tcttgaggat
    62341 ttattcatga gacattatga aggatgagga ggaagcgaaa tgactgataa attttcacgc
    62401 tggtccaccc ttttttctca aaaccttagc cgtgactgga aaaagatcct gatatggcta
    62461 gtcggtgtca ctgctttttc tggtggtttt gtccctgcgt ttgaagaatt gacttccgga
    62521 caaggattag ctggattata cgaaaccttg caaaacccgg caatgattgc aatggtcgga
    62581 ccaactccag cttcgaatgc tgctgagtat actttaggag cgatgtatgc aaatgaaatg
    62641 ttgctttttt gcagtttgtt tgctgctgtg atctctgttt tacacgtggt gagccaaacg
    62701 agaaaaaaag aagaacttgg tctgattgaa cttattcgtt cctttcgagt cggacgtcaa
    62761 gcaaactctt tagctgtcat cgctgaaagt attctgatca atttgctttt agcaatatcg
    62821 atcagctgta cgatgatttt tttccaagcg gattcgatca ctgcagaagg tgcttggctt
    62881 tttgggctat ctgtaggtag tatggggatt ttggggagtg ccgttgcact gtttgctgca
    62941 caactgatgc caagttcagc cagttcgaat ggtctgtctt tagcgttttt aggtggactt
    63001 taccttctac gagcaataac agatgtagct gattcctccc tttcgatgtg gaatccttta
    63061 ggctggtctt atttgaccta tccatttaca actaacaact ggattccttt gctttatttg
    63121 tttttgttca gtctaatctt gataatcagt tcatttgttt tagaggaaca tcgagatttg
    63181 aacaccggct atatccccga acgtgaagga aaaccgtatg ctaaacgttc tcttttatcc
    63241 ttgcgtggtt tattattcta tctaaataaa gggatgacaa tcagttggct tatcactttt
    63301 ggtttgatgg gggcggcata cggctctatc tacgggaata tgcaacagtt tttagaaagt
    63361 aacgacttaa tgaaacagat gtttatccaa tctggcgtct caattgaaaa aaactttaca
    63421 agcacgatca tggtagtctt gatttgtcta gctgctattt taccaattgc tagcatcaat
    63481 cgtttattta cagaagaaac ccacggacgt atgaatcagc tgttcgcaac aaaaatttct
    63541 aggaaccaac tttattggac caatatgtta ctagctattc tatttgctat attggcaatc
    63601 ctgattagtt ctggtgcact agggctttct gctttggctt cgatgaagga tgcgaaagac
    63661 atggatctat tgatgtttct taaagccggg ttcaatttcc tgcctgccgt tttagtgttt
    63721 atcggaatta gtggctttct attcggatgg cttcctcgaa tcggtaaact tgcttatatc
    63781 tacttagctt actcctttgc actcaattac tttggcggaa ttttggattt accagaatgg
    63841 ttttccaaaa cagctgtccc tagctgggtt ccacggatgc caaacgattc ttttgatctt
    63901 tccgtgttcc ttacgttatc atgtatcagc cttgcactgt tcgtccttgg ttctattggt
    63961 tataggcgaa gagacttact agaaggataa taaaaaagca tgtacaattt catgctagcc
    64021 tccaaatctg ggtcaaaaat ccaacatttg gaggtcattt ttttatttac aagtcttcta
    64081 actgctcttt atcaatgatc cagacttctc gattctttct ttttacataa ccattctcct
    64141 ctaataattt aaattttcta gataaagttt cgggggtcgt accgataaat gcagcaagtt
    64201 ctttcatttt cattggcagg aaaaagtgat ccttccctgt cgctttagct aagtcaagca
    64261 gatagacagc gactcgttct tctacttttt ccatcatcaa aaaagttgtc tgctgctgta
    64321 cttttgttaa tttctcaacg ttgattgcta aaagtttcag actaagggcg ggataatgca
    64381 gtaagagttc cgagaaatct ttttgtctca atatgcaaat ttccgttttt tctagtgctt
    64441 cgccaaataa agactcattt ggaacgccta gcaactggct ctctccctca tatccgcccg
    64501 gctctattac tcgtaaaagc tgttcctttc cgctagcaga taattgatac actttcatac
    64561 tgccggaggc cacgatcact aattgaggtt ctccattcgg cgcaagaatc tgttctcctt
    64621 tttccacata gtgatgtttg acaagtgcgt gtactttttc ttgatcttgc tgtactaagt
    64681 gattgaacaa aggaacaaat gaaacacata aatgatcatg actcataatc tctcctcctt
    64741 ctaaacagct atttctaata aagaaacata acttccctta ttccaactga taattttctt
    64801 ttatcatagc ataaacgtca agcgaaccag ataccagcaa atagagtgga tttctttttg
    64861 tcccttgctt tattttcctt cttttgagac cttaagtaat tgattcctat caatttttta
    64921 aatatttact ttttgaataa tcaaaaacaa accttcactc gcaagccgag agtaaggttt
    64981 gtttttcgca ttgcttattt cgtgttgtct attgaaattt ttccgcacct agcttttgat
    65041 atagtggtgc tagttcttgt aaggtttcag cggttatttg ctcaacgtgt tcgatttctt
    65101 gcggatagaa gaatagttta cccatttgaa aggttcggtc tctcatcgct tttttcaact
    65161 cttcttgttt ctttgtatcg gacaaatagt cagacaaagc taagtggtcg tcaagttcat
    65221 gttctggttg cggccatatg taataatcct tgatatcgac tgttttgctc cactctttca
    65281 gtatcgcaag caactggtta tacgctgcaa tgctaccata tcgttcttcg cttttataat
    65341 gttggtacat caaatagatt tggtattgtt tttgattcaa caaaactgct agacaggcat
    65401 tctcgtcttg acgagattcg cttcggtacg cgctccaaaa atgactgcgt aaattccagc
    65461 cattcgtcca gctttcgatt ttaggtttgc tcatgccgta catatcaggc aaaagtgcag
    65521 ctgtctggag ctgaatctct ttccattttt gccagtgttc tttatattct ttcttgattt
    65581 gttcaatctt atctgttgga tggctctctt tgagctgttt gaatgcgtag attggtcgat
    65641 caaacaactg ataatctgat tttccaagtt ccattgtttc gcctctttcc gttatctttt
    65701 gtattctacc tgaattttgc gatatggaca aatagaggtt gtgaaaaagc tgttctgcat
    65761 caagtcataa gaacagccaa acaaaaaact gcgatgagca tcaccgcagt tttattgaac
    65821 ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag aagcagctcc
    65881 tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt ttttagctac
    65941 caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat tccctgcctt
    66001 caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag cttctgcctt
    66061 tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt tttcttgaga
    66121 atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag atccgtataa
    66181 cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt tgaatgtatc
    66241 gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa cccctcttcc
    66301 catgttattt ccatggtctc catcgccaat cggggtatct agatcactga gatattgttt
    66361 gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt gaacggttag
    66421 tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct gtacattgct
    66481 ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag aaagaccttg
    66541 catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga cgcctctttc
    66601 ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag gcgtcccgcc
    66661 cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat ctaaaatctt
    66721 agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac gatagcctgg
    66781 ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga atcctggttt
    66841 ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag ttgcctgtac
    66901 gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg ctgcatcgcc
    66961 cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag tatacgtact
    67021 attttcaacg gcgatatcat catctacgat cacactttct acttcaatat cgtccatagc
    67081 agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct taacgatcag
    67141 caaaactcct tttccactat cggcttcctt aatagccgtc tgaatctgat ctggagttgg
    67201 agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta caaacccggc
    67261 atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt ccgctttctg
    67321 attcttggct acaacacggg aagattctac acgatgaaca agttctggat agctttttac
    67381 taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt ttttcaattt
    67441 attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga gtatcatctg
    67501 ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca tccaccagat
    67561 gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag ttcaataccg
    67621 cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca ttgactgctt
    67681 ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga gaaaaaattt
    67741 gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct gttacttttt
    67801 gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct tttacatagt
    67861 tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata aatttctaat
    67921 tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata ctttaattaa aagagaggag
    67981 tgatcgaatg attacaacaa caacgaacag tgtagaggga aaagccgtag tagcttataa
    68041 agggatcgtt tttggtgagg tcattactgg gatccatgca tttaaagact tggaggcggg
    68101 gttgcgaaat atctttggcg gccgctcaaa aagctatgaa aacgaactaa tgggtgcaag
    68161 agaagaagca cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg atgcgatcat
    68221 tggcgtaaaa atggactatg aagtattagg cagcgacaat ggcatgctga tggtcacatg
    68281 tagtggaaca gctgtctcca cgagataagt aggggacatc tgttttaata actaaagaga
    68341 gatgggacat gtagtggcac tcaagaatta gatttttcag tctaacttcg aggtactcta
    68401 ccttcccatc ctctcttttt tatttctttt tgcggatgat tccgtaagct gctgcgacta
    68461 acacacccgc ccctgctaag gaataactga cagaagctgt tttttcattt gtttttggca
    68521 aagtctgact tgttgatttg gatggctctg tcgtttgctg aactgtttct gtcgtgcttc
    68581 cagaggtcac tgatttttct cctgccgaat gcaaaatatc ttttattcgt tggaaactag
    68641 ctgctaagtc agcttttttt gcttcaaaat cattagcaac tgtttgtgct tcgctgatct
    68701 ttccgtggat tgcttgactg gccgctgtga tatccgcttg agtttgactt agctttccag
    68761 aagctgtttg agcaacctga tttaattgct gattgaattc ttttgcttgt tggatcttcc
    68821 cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc tttttctcaa
    68881 gttgtgcacg gtcatttccc actttatttt taagatcagc taaatcttga tcgattgctg
    68941 cttggtcttc tttcaatttg ttttttgcag cttctaatgc ttcttcgagt tcttttcttt
    69001 tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta tttattgatg
    69061 tatcttctga agatgaaatc gtttctcctg aattatctgt cggtacattg gaagtcattt
    69121 ctgtttccga ggcaccgttt tcagcaccag aagagcttat ggttgttgaa ggttctgttt
    69181 gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa taagaaaata ctggaaacag
    69241 caattggcca gattaacggc ttcattcttt ttttcatcac ttttcccatc cttactactt
    69301 tttaactaat ttcttattat aagcaaaaat gtttggaaga accataaaca caacaggctg
    69361 tttgtctaaa aaacggtgca tcttttattt ttttgtacgt tccgggcttt tccatgtccc
    69421 gcttccataa cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga tcgttatcgc
    69481 gttcactatc cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt tcaattttct
    69541 tcgtcgatca tctacaatca gcgaagctag caactggaaa aatcctgcga ccatttcaaa
    69601 gcagatgaac acaaaactga atgtaaaagt aatataaatc tgttcgtagt ttccttgata
    69661 tacataatgc ccgattaatc cagcaaaaag aactacggaa atacaaaaga atattgacca
    69721 gacaatactc aatgtttgat caatgaagat gatcgttctt ccaatatgtt taaaaggatg
    69781 gagcatcact tttttgaaat tcgtcagcca gacttctgtt ccaccttttg cccaacgttt
    69841 tctgtgtcga tatagcatct tcaacgtcac aggtacttcc atgaaaaaga taatctcagg
    69901 agcaaaaaca gataaccagt catttaactg atgatcccaa gcaatactga tgtcttctgt
    69961 cgctcgatct tgtctgaatc cgcccacatc gatcaaggct tcttttcgat acatcgtatt
    70021 tgctccactg tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt taatgatgcc
    70081 cacgatactt gagaattcaa ccgtttgaga ttttgcaatc aatttggtac gattttggac
    70141 atccatattt gcagtgaccg cagcaatatg tcttgcacca gggcgaatga aataattcac
    70201 atacctaatc agtgcatctg gctccggtac cgtgtctgca tcattgctta aaatcaattt
    70261 cccttttgca aaggcaagtc cgatattaaa cgcgtgggct tttcctttat ttttttcgat
    70321 ccgaaccaca cgaagattag cgtatttttt catcagtcta gctaatattt ctggtgtttg
    70381 gtccgttgaa ccatcgtctg tcactaatac ctcatagttg tgataattca atttcgtcat
    70441 caaatactcg attgtatatt cgatcacaat ttcttcatta tgggctggca ccatgatcgt
    70501 gatgaacggt tcgacactca agggaacatc tacccattct ttttgcttat gtttgtataa
    70561 aaacacataa caccagacac caataaacga tacaaatccg cctaaaatcg gataagaaac
    70621 gagaaaaaaa tacataatag acaaacaagt atctaagaat aaattacctg tatgggtgat
    70681 tgagatcatt tcagttccac cccattttct ttatacaaat ctgcaatgaa tgtcgtatct
    70741 agatttttct cttcggagac agaataaaaa cggaccgttt ctctctcttc ttttgttcca
    70801 aaacgttccg tatacacttc ttcaagtaat tcttgtcttt ttttcaattt ttcatcttca
    70861 tattcaaaag atttatttac ttttttgtat agatttcgat tgttccacct agtcaataca
    70921 aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa gaaatacagc
    70981 attttctttt ctgataaaaa agcgtacaag gaaaaatcct tggctctttc tggtctcgtt
    71041 aatgggaaaa agacccatat aaaaggaaga acaacgatca tccatcctaa aagagccact
    71101 aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa aatacttatc tttcaccaaa
    71161 attgatgcaa cttcttctct tgttactttt ttcatatcct cacctcacgt ttttttgtta
    71221 cgacgtagga gtttaccata catttcattt cttttcacat aaagtgttcc taaatttttc
    71281 ctaagaaaaa gaacaaaaca tttctcatca gcaaatgaaa aatattttgt tctttttatc
    71341 agttgttctt tttatttttt atctcttaat tatgttgctc tttccaatac gacaatgctt
    71401 ttcattcctc tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc atctccatcg
    71461 ttttcaaatg gatataagat tacatgatca aattccagtc cttttgctag actcaaagga
    71521 atgatttggc agttttctaa tccatatgaa ttttttttaa tcgattcttg tagcgtttgg
    71581 gtttcctctt ctgtttgggt gataactgcc gctttttctc cttttagaga aaccaaaatc
    71641 tcagctagta ttttcaacaa ttcttcagag gatcgaaaag agcggtattc gggttctttt
    71701 ccttgcggtc ggatcgatac gatgtcgatc ttctcttgat ccacagcata ggttttgaaa
    71761 agctctgtga ttgctccgct agaacggtaa ctatttctaa gatcataccg atggatcggc
    71821 agatggtagt cgtcgaaaca acgcataata tcagcaaaag tagtggaaga attaaagatt
    71881 gcctgattct cgtcacctac taaagtaaag tgagtttttg gaaataattc gatcaataga
    71941 cctaattgtg ccagggtata atcttgtact tcatcaatca atagatagcg aaatgctggt
    72001 acatttacct tttcaacaag taaatgacga atagctaata taatgactgc ttcatcaaca
    72061 gtacgtgtgg gttgccaagc ataagctgag ccatatcgtt tttcataaat tctttcaaac
    72121 aactgatgct catccaccca tgccatttct tcgatttgtc tggttatttt tcgatatttt
    72181 ttttgtaaaa gtttccttgc ataagctaca atactttgtt ccgaatcatc agaaatcaat
    72241 ttaccgaata attccaactg cctatcttct gtcaacgaga gtacctgatt ctgcacagca
    72301 ggactttttg cttgtttcaa aagatgattc ttccattcgc tcatcagtgc ctttttcgtt
    72361 gcttgaagcc tatcgatcaa tttaggatga gcaggaactt tctcatatat tttttcgatc
    72421 gtttttttag aaaggactgt ttttccattt ttagtaagtc cacggataaa attcggatga
    72481 tctaaaaaca aaggatctga ttgttttaga ttatcgataa actttttgct acgcaaacgt
    72541 tctgtctgct ccgatacatt ttctcccgtg atacgtttga aataagcctc ttcaccttct
    72601 atttcctctg ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag tggatttctt
    72661 tcacctaatg atggtaggac attcgaaata tagtcgataa accgatgatt aggtgataga
    72721 atcagcaagt catcggatgt catcgtatct cgatataaat agagcagata cgcaatacgc
    72781 tgcatgatag cagaggtttt accacttcct gctacgccat ttaccaagat attttgactt
    72841 ttcacatccc gaatgattgt gttttgttcc gattggatcg tagcagtgat agccttcatt
    72901 tcattggtct catcttggcc taagacttct aataatacgt catcttgaat agctacagac
    72961 gtatcaaaaa acttgaccaa acgatctctt tccaaaagaa attgtcgtct ttctttaata
    73021 accgtatcga ttttattttg acgaaccgta taagaagatg gaccaatcat gttattgtaa
    73081 aaaagttcgg caattggtga acgccaatca taaacaagat tttcatctgc atcatctgtg
    73141 aaatttgctg taccgatata aaaggattct tttggctcat tatccagaaa gtctacaacg
    73201 acttttccaa agtagggact tttcaataaa cgttcgatct ttgctagctg attttctgcg
    73261 tttttctgtt tgatattcat ctgatcgatt tctctatttt tcatttcaat catcgaaaaa
    73321 gtatccaggt tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc catttctttc
    73381 aacgactggt ttccttctat ttttgccgta tccatcgatt cccgcagttc gtttttttta
    73441 tcaattagct tttgatacac gttcttcaga tatgcttctt ctaactgttt ttgtgtgttg
    73501 accatacaca ttctcctctt tttttaaagt ggtggaaccg attagtttac ctcttttaag
    73561 agtcgaatgc aagcttttta acgagtatca tttgttagga tatatcagtt cttcaggtcg
    73621 gacagtttga tataaagaaa atgcaccatc aaggtttttt acgtggtagc cagactgctt
    73681 caagattctt tccgctagat agctacgtaa tccgctatga cagctgacga tatattcttg
    73741 ttggctgtct aattcattta atcggtcccg aagttcattc aaagggatat gtttggcttc
    73801 cttgaatttg cccttttcca attcttctgc tgttcgcaca tctaataaaa tttttccttt
    73861 tgctagttct gtcggcaatt catgccattg tacggtttca ctaagccctt ctgcaatgtt
    73921 cattgctgca tatcctagca tgtttactgg atctttggct gagccaaatg gcggtgcata
    73981 cgtcaattct aattccggta aatcgaagat cgtcaaatgc cctttgattg ctgttgctaa
    74041 aatatcgatc cgtttatcca caccttttgc cccaacacct tgtgcaccat aaatctcgcc
    74101 agtctctgga tggaagatca acttcagtaa aatatctgtt gcttctggat aatagcttgc
    74161 atgatctttt cctgatacat gaatgacttc aaaagacagt tcgttttctt tggctgttct
    74221 ttcacttaaa ccggtggatg cggcagtcat tccaaacgtt cgtataattg ctgttccaat
    74281 accgccttga tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc taccttgacg
    74341 attggctggt gaagcaagag aaataagtgc atcttttcca gtgatttgct gttttacaac
    74401 gatagcatcc cccaccgcat aaatatcagg aatattcgtt tgataacgtt cgtctactag
    74461 gatacccccg cgtatgccta attcgattcc agcggtcact gctagctggt tatcaggacg
    74521 aacaccaatg gataaaataa ctaaatcagc agggatggta cgcccatcat ccagtcgcaa
    74581 tcgtttcccg tggtcttcaa atccagcgac agcatgagaa gtaatcactt ctactccttg
    74641 atgagacaat tctttttcta taaaagctgc catttcttca tccagtgttg gaagaatatg
    74701 tggtgcttgt tcgatcacca tgacttctaa acctcttctt tttaggtttt ctgccatttc
    74761 caatccgatg aatcctgcac caacgatcac ggcacgcttt ggctgttttt ctaatgcatg
    74821 catcacttta tcgatatctg gtacatttct aatagaaaaa acattgtcgg cttctgttat
    74881 ccctggaatc gatggaacaa ctggttttgc accaggagat aaaaccaatg catcgtactg
    74941 ttcaatgtgt ttttgaccct catgtacgac ttctaccgtt ttgttttcgg gaaagatggc
    75001 tgtcacttca tgatttggac gcacatctaa acaaaaacga gcggataatg attctggcgt
    75061 ttggacaaga agattttccc gttcactgat ttctcccgaa agataataag gcaagccgca
    75121 atttgcaaaa gaaacatacg gtcctttttc aaaaacaacg atttctgcat cttccattaa
    75181 tcggcgaagc cgtgtagcag cagacattcc acctgctaca cctccgacaa taacgatttt
    75241 catcttattt ccctcctact gttcgatcaa accattgatt catgccacca cgtacattca
    75301 ctacatcata accagatttc tttaattctt tagctgcttg tttactcctc atccctgatt
    75361 gacaaatcac atagacggtc ttttctttgt ctccatcata tctgtttata ctttgtaatg
    75421 gaacattttt agctcctttt atatgcccgc cccggtattc tgcaggtgtt ctaacatcaa
    75481 gtaacgctgg attttccaaa agcttttcct ttaattctgg gactgttatc gattttattg
    75541 attgaaatag caaattcatt ttctgcacct catttacatt tttataaata cctcacgggg
    75601 tatattaaac aggagcttta ttaatatgtc aaccagaatg actcttcatt tttttcacta
    75661 ctttttatat ttgcataaaa taccgataag gggtattatc taattataaa aatagaggag
    75721 aatgtgttat ggagattgat aataaaaata tcctgaaccg tttgaaacgt acagagggac
    75781 aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga atgtatggat gtcatcactc
    75841 aattaagcgc tgttcgttct agtattgatc gcgtaatggg catgatcgtt gcagataatc
    75901 tgaaaaattg ctttgagaaa cctgatagta atccagaaga acaagcggct aaactagaac
    75961 aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt caatgaaagt cgttttccat
    76021 attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct gataaaagct
    76081 tctccagaaa cgacgattgt cgtatctgta aatggcatcg cgattaccgg ttatctagac
    76141 tctgccaatg cagaattctt agatttgcaa ggagtcgttt ttcacgcttg cgccaatgct
    76201 atgcgtgcta atcatatttc agaaagttct ttacctgaac aagtaatcgt tgtaccagca
    76261 ggtgtccttg atttagttga actgcaatcc caaggttatg cttatatcaa gccttagtca
    76321 gtagaggttg cgacagaagt gatcagcttc aagaaatttc ggcttatttt tcgaagaagc
    76381 tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc aagtaataag
    76441 aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttgggcttat taccgcagat
    76501 gcaagctttt gaacaccgtt taaggaagag gttgtgaaat cagcggtttg acctaagtat
    76561 tagagaaaaa aacgggaaaa tagctcctca tattttgccg ttttttgagc taataccgaa
    76621 ggtcagctga ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt agtttcgagt
    76681 attaagaaaa attttgtgaa aatggatttc tacatttttg cataaatttg gcttaatacc
    76741 gagaaactgg cttttgaata ccgtttaagg aagaggttgt aaaatcagcg gtttgacctg
    76801 agtattagag aaacaaacgg aaaaatagct gctcatattt tgccgttttt tgagctaata
    76861 ccgaaggtca gctgattgaa caccgtttac caagaggttg tgagcctacc atttaactcc
    76921 gaatcacaag aaataatttc aaaaacaact tctcctgttt ttggttcggt tggcttatta
    76981 tcgagaagta gctactggga gtctatttat caaaaaaagg aatgcgacat aggtcacacc
    77041 ccttttttat tttctttgaa acatctttcg gtactcgaca gcatacaaga caaaagattg
    77101 taaaagaccg taagtgatga cggcaaaaat gactctcacc cacagcgttg gtaattctgg
    77161 gaaaagcagc aaaaggatca tgccaacata gatgacagca gtcgctactt tgccatacca
    77221 cttcgctcca cttaatttga tatttttaga tagcccgatt aatccagcta caaacataaa
    77281 gccttctttt atcaagaata gaccaaacaa cagccaaagc cacggtcgat accatgccat
    77341 agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca ccttccctaa
    77401 gtctgttacc tgattatatc tccgagcaat gaccccgtct agataatcag acaaacctga
    77461 gacaacaatg accgctcctg ctatataaaa tgactgtctc agcacaaaat aaagataaat
    77521 cggcagcaaa aaaatacgga atatcgataa aagattcgga atcgtttgta tttctttacg
    77581 ccagtctgtg cttaatttca ttttatcgcc ctacctcctc agcactcttt tctttttatt
    77641 ttagcttaca cggggaataa aaccaatgat ttcactgttt ttatttttct ttacagacac
    77701 tgatattatg agctaatttg agataatcac ttgctgtttt catcgtttac tcttctttct
    77761 tcctgtcgtt tgcgcatttc ttttactagt actttatgag ctttttttga tcctcgctct
    77821 aggtcacgct gccgtttacg tgttttataa tcgataaaca gactgtctaa atcgtaacct
    77881 gtcgggtaca gatcttctgc tgaacgcacc agacgaacac gctgacgagg cacgtggatt
    77941 atttttttgt ctaaatatac atcaatcgta tcttcaccta tatcttcata aactaacgct
    78001 tctttttggt attcattgac aaatatccga tcaccttttg caaagagctg acgttctttt
    78061 ttattctcct tcctatttgt aactgctgtc ttaggaagaa attctttttt ctgtgtccga
    78121 taggcttttt ccgccaagta acgctctgct tgatggatca atttcatcga catatccatt
    78181 ttatgagcaa tccacaaagc ttggctttct cctgtttctt ccaacccgca actgatattt
    78241 aggacgaagt gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc
    78301 ggcgaatttc ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata
    78361 catagattcc atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc
    78421 aatctcatct aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt
    78481 ttgcatatga ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc
    78541 gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc
    78601 gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc
    78661 atttgctccg gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac
    78721 cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt
    78781 gattctaggt gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc
    78841 taacaccgtg atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc
    78901 agtcaatgcc gccaggactt gatattcttc actgatttct tcagctttta acaactgata
    78961 gtgttcattc aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga
    79021 ttcatcgata atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata
    79081 gtattcgcct ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg
    79141 caagaatttc agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac
    79201 tttacgcaaa ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat
    79261 caactcttct acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt
    79321 ctggtattga ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc
    79381 atattcgatc aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc
    79441 atcgatcctc ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc
    79501 ttctcttgtt tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc
    79561 gctgattcgt tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg
    79621 atcaaattgt aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat
    79681 acctgtccca aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa
    79741 aagtcatgtc ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc
    79801 ataagcacac caaataaact ttgtttattg gctattcgac ttactgatta gataccagct
    79861 acactcacaa aacatacccc attcttctag gtcttattta ttttccactt aactatagca
    79921 agacacattt tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag
    79981 acacctaccg cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc
    80041 caatctgaag ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag
    80101 tcccgagcag ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag
    80161 atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct
    80221 agcaacccaa gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa
    80281 ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca
    80341 ttccaatgtg gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact
    80401 tgcgcaatga tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct
    80461 agagagactg caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt
    80521 gttacagcga gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt
    80581 ttgtttaaat tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca
    80641 ataaactccc ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc
    80701 cgcagatctg attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct
    80761 tctgtccatt caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc
    80821 tgatctcgtt caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa
    80881 accatttctt cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga
    80941 taaggtgcca tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca
    81001 gcgatataag tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct
    81061 gtcactgttt cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta
    81121 gagtcgattt ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc
    81181 aaagctcctt ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa
    81241 ccatctggtt ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca
    81301 cggctgtttt ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc
    81361 ttatattcac ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta
    81421 tctccaccat tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag
    81481 cttgcttcga tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc
    81541 aatagataat ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc
    81601 gtatctgtcg taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca
    81661 tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca
    81721 actaaatgga tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag
    81781 tttcgtactt tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg
    81841 atcacttcat ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat
    81901 aaaaagccaa ccgctttcat tttacttact agataagccc catcttcggc agcctttttg
    81961 atattatcaa tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct
    82021 tttagccctt cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta
    82081 taattctcat atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact
    82141 ttgaccgaga acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt
    82201 ggatctggtt ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg
    82261 tcagctacta cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg
    82321 gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt
    82381 cctgatgctg ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac
    82441 agatccaatt cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac
    82501 atatggattt cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt
    82561 gtaaagtttt tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata
    82621 aaattcgcgg atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc
    82681 tgcccctagc gccgttgttt ccaagttttc tgctcgttgg atcggtacat tcaagatatc
    82741 tgtttggaat tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg
    82801 aatcttgatt ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat
    82861 cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat
    82921 agcccctcgt gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac
    82981 ataaacctca tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg
    83041 caacatttga agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc
    83101 ttctaaagca tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt
    83161 tttggataat tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt
    83221 attcttgacc atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc
    83281 tgccatccct gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata
    83341 aacttctgag ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa
    83401 aatctcctga tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga
    83461 gtaatcagta acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt
    83521 tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat
    83581 ccagcgtatc ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa
    83641 aaattcttga tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg
    83701 ccaaacaact gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc
    83761 acgctggttc gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa
    83821 cgctccggca ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca
    83881 actttcttgc gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt
    83941 cttgtcaaag ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt
    84001 tgattctgtc attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc
    84061 atactactgt tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca
    84121 gttttttatt atgaagctat tttttaatca attcttttct tttttcagcg tctctattct
    84181 tcttttcaat cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg
    84241 ttttttgttt ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact
    84301 tccaatctca ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat
    84361 acgataaatc ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa
    84421 atcgatcatc aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc
    84481 agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt
    84541 taaggaatgc ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc
    84601 gatctttcca acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc
    84661 cttttcttct ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat
    84721 gtaagtatct aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa
    84781 tatttcttcg tctactatcg aaaaagaaac aaaaaagctg taaaacatca ttgtttcgta
    84841 tacattcagt tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg
    84901 ataaagccgg tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt
    84961 ttcattcaat agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga
    85021 tgccgtaaac gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg
    85081 tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata
    85141 aaaatatcgt atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa
    85201 ttcctctaag agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt
    85261 aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat
    85321 cttaaacttc aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc
    85381 gtccaacaca agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat
    85441 tcggccaatc aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac
    85501 atgctctctc aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat
    85561 caggatctgt aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg
    85621 ccacctttga ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt
    85681 cttctaactg ttcaaacgtc catccttctt ctactgcaat tgtcagttgg tcaagaaggt
    85741 ggaacatctc ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata
    85801 gaaatttgag tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg
    85861 tttcaatttt ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga
    85921 aatagccaaa ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc
    85981 cactaattgt cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat
    86041 acattttttc atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt
    86101 ctttttgaga cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta
    86161 attgttcttc ccacatctga ttgtcgggac gagaatgatt agccagcaaa acaagatcat
    86221 agttctcctt tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa
    86281 cttgttcttc taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg
    86341 tcatttcagg atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt
    86401 gtttttttct tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa
    86461 ttgcaatttt ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt
    86521 taactgggtg aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag
    86581 tcgctagaat caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt
    86641 ctttcaatcc tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt
    86701 cttctttcgt gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat
    86761 tgaacccgct gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg
    86821 ttggcatcaa ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa
    86881 ttacaatgtt catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc
    86941 agaaaacaca gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat
    87001 caaccctctc catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta
    87061 attgaagagt atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa
    87121 actcgccttt tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt
    87181 tagcgtgtgt aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct
    87241 tcaacaactg cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc
    87301 ccatcaattt gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct
    87361 ttcaaagcat cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct
    87421 tcattactga acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta
    87481 actggcaaca atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag
    87541 atttgtgaag cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg
    87601 ccaccagcat aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta
    87661 tttcccaagg cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc
    87721 atattcaaaa cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct
    87781 gcatcgatcc cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa
    87841 cctgtaggac gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc
    87901 attttcccat cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat
    87961 ataatgggct tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt
    88021 catcctcacg gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc
    88081 caattgctgc aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc
    88141 caccgacacc aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg
    88201 atttatcgtt gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca
    88261 gataatctag ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga
    88321 tacctggtgt ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag
    88381 catattgttc aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat
    88441 tttccaagct accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt
    88501 ggatcaaata cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc
    88561 tacgtgtggc tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc
    88621 gtggtgtttc cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc
    88681 cagataaagt tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg
    88741 cgccttctcc ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag
    88801 cgatgctgag ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa
    88861 taacagcacc aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca
    88921 ctccattgaa aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt
    88981 gaactcctga aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa
    89041 ttcgctctga ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat
    89101 gcaactaatt tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt
    89161 gtctgggcta attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt
    89221 tcttcccagc taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact
    89281 aatgacaagc gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca
    89341 aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt
    89401 ccatcttttt cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt
    89461 ctaaacgctt catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa
    89521 agacattcat ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac
    89581 atatcatagt atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg
    89641 tctagatttg aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa
    89701 ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa
    89761 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa
    89821 actggctttt gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa
    89881 taagaacagc caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat
    89941 ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc
    90001 acaaggagca atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg
    90061 aggagttggc aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc
    90121 acagtccctt atttcctttt attataggat ttcgccattt gattggatga cttgtctgta
    90181 ccaatcaaat gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc
    90241 aacatagata tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat
    90301 gcagccccac gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg
    90361 ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc
    90421 tactttgtct atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc
    90481 atataattga ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact
    90541 tgatggaaga tacggatttt taattcctcc agttattaag tttccttgga ccccttccca
    90601 attttctgga tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac
    90661 agggtaggct gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc
    90721 ttcaaacatt cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa
    90781 caaagaagtt ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg
    90841 aacaagaagc tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca
    90901 agcttttgta gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg
    90961 ttccaaatgg ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac
    91021 acagttgatc tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac
    91081 tgtttcagcg aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc
    91141 tgtaaccaaa tataacggca tttcataatg ggaaagagaa acgactggct caatcccata
    91201 ttttagacat tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc
    91261 ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc
    91321 catttcagca aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct
    91381 tttaggataa taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat
    91441 catatgccat tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc
    91501 agacacagat aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt
    91561 cgctccgccc cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct
    91621 agttttcttg tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta
    91681 tttgtaacaa taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct
    91741 acttccatca ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag
    91801 tatttgccat ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct
    91861 tcactgacta ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca
    91921 gaactgtaga tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta
    91981 gcaaatgttg gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt
    92041 aacactatat cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt
    92101 agcatacagg aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca
    92161 ccaatataaa tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct
    92221 gcggcaccag ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt
    92281 ttatatttaa gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata
    92341 aatgcagata aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt
    92401 actgctcctg cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg
    92461 tagaaagaaa gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact
    92521 aacacttgcc aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca
    92581 gctaaatatc cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat
    92641 aaaataattg ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt
    92701 tcgtgcaagc ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt
    92761 gctggaataa ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag
    92821 gttatgctat cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc
    92881 atagcgatga aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc
    92941 aagaaataga aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta
    93001 gttaataaat tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc
    93061 aagacgccta aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg
    93121 ccttttttct cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg
    93181 gataatacgt catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca
    93241 gcttcgacta cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta
    93301 ttatctttta gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct
    93361 tcgcctccga caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc
    93421 cccttgttaa ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac
    93481 ggtttctcca ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta
    93541 tggtttactt agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc
    93601 ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt
    93661 atctcaagct gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac
    93721 aaagtctcgg catttaacgg aagcataact aggtcagaac taggagagat tagttgctga
    93781 atggaatcta tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc
    93841 agttttgttt tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc
    93901 tcccaagatt caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa
    93961 ataagctctt gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt
    94021 ttgaaaagat acactggttc tatccggcat tctttgatcc acatgttcat actttgttga
    94081 atctgttttt ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc
    94141 ccttgttttc gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca
    94201 gctaaattgg gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct
    94261 aaaggataac gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc
    94321 atcctcgttt tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac
    94381 gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct
    94441 atttctttgt ttttttcatc attaatcatc aaataattta actctaatag tcgattttca
    94501 attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg
    94561 agggtctcta ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct
    94621 cttcgaagaa tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca
    94681 atataattat atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg
    94741 gaaaaaattg tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca
    94801 acaagagata agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc
    94861 gacgaaactt ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt
    94921 tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca
    94981 tacccttttt gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa
    95041 tagattttat gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat
    95101 tctaaaaaat aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca
    95161 agcatatttt tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat
    95221 tcttcgcttg ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt
    95281 ttatttaagt ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc
    95341 aaatgacaat agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag
    95401 gtttttacca ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct
    95461 cctaaagctt ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct
    95521 catttcccct gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat
    95581 ttggcaacca tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca
    95641 acgttttgga tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt
    95701 ttcaactatg aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa
    95761 ccctagagga tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt
    95821 atcgaaacca tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc
    95881 gctacgataa taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc
    95941 tccatctaac attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg
    96001 ataatgccct cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga
    96061 accaaataca ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca
    96121 cataataccc atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt
    96181 cgctagaaac atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg
    96241 ttcaagttta tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa
    96301 agcattattg atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa
    96361 gacggtctgc gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata
    96421 ctctcctacc aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc
    96481 atatcgattt aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt
    96541 ctgttcgtct cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa
    96601 acctaactcc ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc
    96661 atgagaaaga taatagagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt
    96721 ccactgaata ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa
    96781 tttgccgtct tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa
    96841 aaaattttct ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct
    96901 tacttgaagt atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga
    96961 agaaaaaaat tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac
    97021 gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca
    97081 caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat
    97141 ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca
    97201 tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct
    97261 caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca
    97321 aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg
    97381 cattgatttc tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag
    97441 catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga
    97501 ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga
    97561 ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact
    97621 cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct
    97681 tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat
    97741 cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg
    97801 ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt
    97861 cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag
    97921 gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca
    97981 gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc
    98041 cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa
    98101 agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct
    98161 gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc
    98221 ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct
    98281 cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt
    98341 tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga
    98401 tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac
    98461 cgaattctgc catgattgct tgataattac tgatatcata cccattgtca tcattcggag
    98521 actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc
    98581 tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg
    98641 gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat
    98701 aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt
    98761 aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc
    98821 tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat
    98881 ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac
    98941 ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga
    99001 tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca
    99061 gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat
    99121 agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca
    99181 agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt
    99241 gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg
    99301 atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga
    99361 tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag
    99421 aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta
    99481 cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc
    99541 ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag
    99601 aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg
    99661 cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag
    99721 ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga
    99781 ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg
    99841 tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac
    99901 cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc
    99961 gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg
   100021 ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc
   100081 caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa acacagccga
   100141 tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta
   100201 atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca
   100261 gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt
   100321 ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat
   100381 ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat
   100441 tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca
   100501 tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct
   100561 agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg
   100621 aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt
   100681 tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca
   100741 ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt
   100801 tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc
   100861 agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc
   100921 catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc
   100981 taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc
   101041 ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat
   101101 ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag
   101161 catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg
   101221 tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca
   101281 agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa
   101341 aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc
   101401 atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt
   101461 caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac
   101521 cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag
   101581 acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc
   101641 atcggaatct gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag
   101701 atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat
   101761 actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg
   101821 tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc
   101881 ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct
   101941 tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac
   102001 gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca
   102061 gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc
   102121 ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat
   102181 ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg
   102241 agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat
   102301 gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa
   102361 tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc
   102421 tcttgatcga tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt
   102481 ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt
   102541 ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc
   102601 gtcatttttc gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg
   102661 ccttttttga caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat
   102721 tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg
   102781 ttttctcgaa tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg
   102841 atttttttat tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg
   102901 attgctcggg cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg
   102961 attccaggat tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact
   103021 tctttgaatt cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga
   103081 aggatttcag ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt
   103141 gttgcaaatg caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt
   103201 atattctttt cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta
   103261 aaataagtaa ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac
   103321 taaaaaatcc catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga
   103381 tcgcacaagc aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat
   103441 tcgtatcaaa aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga
   103501 cttctttacg cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg
   103561 accattcttt cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg
   103621 cacgaacatc tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt
   103681 cttcgaaagt ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct
   103741 caaaacgctc tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga
   103801 gtagtctttt gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg
   103861 tatgactatg taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa
   103921 tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg
   103981 ggctgtgaat aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa
   104041 aaatatgacg agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc
   104101 acaacctccc cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc
   104161 atcaaacatg agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag
   104221 gctcacgacc tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct
   104281 ttgttactta ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac
   104341 ttgagtatag tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt
   104401 tcggtcggcg atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg
   104461 atcttccaat acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga
   104521 ttcacgatca cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat
   104581 cgtgatcacc tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc
   104641 actaaaagga tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc
   104701 tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt
   104761 aaacccgatg gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc
   104821 tggaaatcgt tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc
   104881 cagaagaatg aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt
   104941 tcataaacag tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca
   105001 cattatggcg aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg
   105061 tccagttgac taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt
   105121 caggataatc ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta
   105181 ctgcatcgat ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat
   105241 gattcgtacc gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg
   105301 cgtcttccgg tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac
   105361 cgccgattgt tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta
   105421 ctgcttttgt cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag
   105481 aagtatatcc gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt
   105541 gcaagactgc atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat
   105601 gcatgccgat atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt
   105661 cttttgctgc gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa
   105721 ctgtaaaatt caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt
   105781 attgccacct tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc
   105841 atctcatcca ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca
   105901 tctgcacgct tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt
   105961 ttattttcca aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa
   106021 aatttaagtc caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa
   106081 atctcttgtt tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg
   106141 gctttttctt cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca
   106201 aacatcattt tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca
   106261 tataagagtg ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc
   106321 gaattaaaga tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata
   106381 ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct
   106441 ccggcaaaga aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa
   106501 gtaaacatgg caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt
   106561 gggctataag gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc
   106621 atagctatcc ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc
   106681 aatacataca cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt
   106741 tgatctcctg ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat
   106801 agcgggacca tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc
   106861 ggcaacttga tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca
   106921 acaccggctt caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat
   106981 ccagtgattt tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct
   107041 ccgataaaac caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag
   107101 actgtcgtca tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat
   107161 tttgtgaaac gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata
   107221 tctctaccta aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg
   107281 atcccttgca tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg
   107341 atgatgctcg aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag
   107401 atgacttgtc ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg
   107461 gtgatcgatg cctgagtcaa aatattttcg atgataaaat tgataacgcc catgttttga
   107521 tgtgcacccc aaaagttaga ctagaaatct aattaaaagg ggtgcttttt ttgcggaaat
   107581 atacatttgt ttttaagaaa aaagtagttt cagactattt aaataacgaa ggcggctaca
   107641 aatatcttgc acataaatat caaataaatc gtacactggt tagacattgg gtaaggattt
   107701 ataactatca tggttgggaa ggcttggttg gaggtggcaa aagctacact acaaaattta
   107761 aacttgatgt tatagaatat atggaaacaa atggtctttc tatccaagaa actgctaaaa
   107821 aatttaatat cggttcaaat agaactctaa gtaaatggat agagcaatat gaagaaggcg
   107881 gtgcttcttc acttgagagc caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa
   107941 acattcctaa aaaacttaaa gatgagtctc ttgaagaaga agttattcgt ttaagagcag
   108001 agaacgcata tttaaaaaag ttagaaacct tgattcaaga acaagatttg agcaagaaga
   108061 aatcaaggtt aaaacaatcc ttcagctcaa aaaagaattt aaattgaatc tattactttc
   108121 aattgcacaa ttagcgaagt caacttatta ttactgggta aaaaaattag ataagccaga
   108181 taaatatagc aagataaagc aagaaattac agcgattgta aaagaatcaa gaaactctta
   108241 tggttatcgt agagtcactt tagcgttaaa gatgaaagga tatacaatca atcataaaac
   108301 agttagaaaa ttaatgtccc aaatgggact tacctgtcaa atccgaataa agcgatataa
   108361 atcttataaa ggaacagtag gaaaaattgc caagaatgtg ttaaaacgaa atttttcagt
   108421 agatacaccc aataaaaaat gggtgacaga cgtcactgaa ttcaagataa aaggaagaaa
   108481 aatctatcta tctcctatac ttgatttatt taacggagaa ataattagtt atagtatatc
   108541 aactagtcca acatataaac tgattgaaga aatgcttcaa caagcaatta aaaaaaaggg
   108601 aactgagggt tcactgattc tacattcaga tcaaggatgg cagtatcaga tgccacaata
   108661 tcaaaaaaaa ttaaaagaga ataacattat tcaaagcatg tctagaaaag gaaattgctt
   108721 agataattct gtaatagaaa atttttttgg tgtgcttaaa tcagagtttt tttatcgaga
   108781 aaaattccga tcaattgaga tatttcaaag taaattaaat gagtatatta ggtggtataa
   108841 taacaaaagg ataaaactaa agttaaatgg actatctcca gtagaatatc ggaaacagtc
   108901 cattaaatag tt
//
LOCUS       DAZKSU010000002        79173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000002 DAZKSU010000000
VERSION     DAZKSU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.002"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..316)
                     /locus_tag="ACG8Z3_000095"
     CDS             complement(26..316)
                     /locus_tag="ACG8Z3_000095"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0822449.1"
                     /translation="MIDEKKCLKNLLEDMDDIEEKLSKWTRKTNIFQILKLSKNEIRH
                     SNFLAFLFAPNETHNLSDEFFKMFLKRYIDSNDDTKVAINYFDALLNSYPWC"
     gene            complement(519..998)
                     /gene="rlmH"
                     /locus_tag="ACG8Z3_000096"
     CDS             complement(519..998)
                     /gene="rlmH"
                     /locus_tag="ACG8Z3_000096"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0822450.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1426..2715
                     /locus_tag="ACG8Z3_000097"
     CDS             1426..2715
                     /locus_tag="ACG8Z3_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0822451.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2754..3359)
                     /gene="ytpR"
                     /locus_tag="ACG8Z3_000098"
     CDS             complement(2754..3359)
                     /gene="ytpR"
                     /locus_tag="ACG8Z3_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0822452.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(3555..4019)
                     /locus_tag="ACG8Z3_000099"
     CDS             complement(3555..4019)
                     /locus_tag="ACG8Z3_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0822453.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(4041..4361)
                     /locus_tag="ACG8Z3_000100"
     CDS             complement(4041..4361)
                     /locus_tag="ACG8Z3_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0822454.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(4504..5583)
                     /gene="pepA"
                     /locus_tag="ACG8Z3_000101"
     CDS             complement(4504..5583)
                     /gene="pepA"
                     /locus_tag="ACG8Z3_000101"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0822455.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5783..6133
                     /locus_tag="ACG8Z3_000102"
     CDS             5783..6133
                     /locus_tag="ACG8Z3_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0822456.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            6318..7271
                     /locus_tag="ACG8Z3_000103"
     CDS             6318..7271
                     /locus_tag="ACG8Z3_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0822457.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            7284..9071
                     /locus_tag="ACG8Z3_000104"
     CDS             7284..9071
                     /locus_tag="ACG8Z3_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFQ0822458.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            9058..10260
                     /locus_tag="ACG8Z3_000105"
     CDS             9058..10260
                     /locus_tag="ACG8Z3_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822459.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(10336..11754)
                     /gene="pepV"
                     /locus_tag="ACG8Z3_000106"
     CDS             complement(10336..11754)
                     /gene="pepV"
                     /locus_tag="ACG8Z3_000106"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0822460.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            11903..12748
                     /locus_tag="ACG8Z3_000107"
     CDS             11903..12748
                     /locus_tag="ACG8Z3_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0822461.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            12752..13192
                     /locus_tag="ACG8Z3_000108"
     CDS             12752..13192
                     /locus_tag="ACG8Z3_000108"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0822462.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            13242..13436
                     /locus_tag="ACG8Z3_000109"
     CDS             13242..13436
                     /locus_tag="ACG8Z3_000109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822463.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            13572..14258
                     /locus_tag="ACG8Z3_000110"
     CDS             13572..14258
                     /locus_tag="ACG8Z3_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0822464.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(14666..15355)
                     /locus_tag="ACG8Z3_000111"
     CDS             complement(14666..15355)
                     /locus_tag="ACG8Z3_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822465.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(15330..17459)
                     /locus_tag="ACG8Z3_000112"
     CDS             complement(15330..17459)
                     /locus_tag="ACG8Z3_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0822466.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(17507..18844)
                     /locus_tag="ACG8Z3_000113"
     CDS             complement(17507..18844)
                     /locus_tag="ACG8Z3_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0822467.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFCGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(19096..19455)
                     /gene="rplT"
                     /locus_tag="ACG8Z3_000114"
     CDS             complement(19096..19455)
                     /gene="rplT"
                     /locus_tag="ACG8Z3_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0822468.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(19508..19708)
                     /gene="rpmI"
                     /locus_tag="ACG8Z3_000115"
     CDS             complement(19508..19708)
                     /gene="rpmI"
                     /locus_tag="ACG8Z3_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0822469.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(19780..20304)
                     /gene="infC"
                     /locus_tag="ACG8Z3_000116"
     CDS             complement(19780..20304)
                     /gene="infC"
                     /locus_tag="ACG8Z3_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0822470.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(20348..20473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            20624..21256
                     /locus_tag="ACG8Z3_000117"
     CDS             20624..21256
                     /locus_tag="ACG8Z3_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0822471.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(21258..22163)
                     /locus_tag="ACG8Z3_000118"
     CDS             complement(21258..22163)
                     /locus_tag="ACG8Z3_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0822472.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(22248..22907)
                     /locus_tag="ACG8Z3_000119"
     CDS             complement(22248..22907)
                     /locus_tag="ACG8Z3_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0822473.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            23223..24167
                     /gene="mvk"
                     /locus_tag="ACG8Z3_000120"
     CDS             23223..24167
                     /gene="mvk"
                     /locus_tag="ACG8Z3_000120"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0822474.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            24160..25137
                     /gene="mvaD"
                     /locus_tag="ACG8Z3_000121"
     CDS             24160..25137
                     /gene="mvaD"
                     /locus_tag="ACG8Z3_000121"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0822475.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            25150..26235
                     /locus_tag="ACG8Z3_000122"
     CDS             25150..26235
                     /locus_tag="ACG8Z3_000122"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0822476.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            26232..27296
                     /gene="fni"
                     /locus_tag="ACG8Z3_000123"
     CDS             26232..27296
                     /gene="fni"
                     /locus_tag="ACG8Z3_000123"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0822477.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(27545..28348)
                     /locus_tag="ACG8Z3_000124"
     CDS             complement(27545..28348)
                     /locus_tag="ACG8Z3_000124"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0822478.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(28368..29210)
                     /gene="kduI"
                     /locus_tag="ACG8Z3_000125"
     CDS             complement(28368..29210)
                     /gene="kduI"
                     /locus_tag="ACG8Z3_000125"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0822479.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(29226..30233)
                     /locus_tag="ACG8Z3_000126"
     CDS             complement(29226..30233)
                     /locus_tag="ACG8Z3_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0822480.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(30240..30884)
                     /locus_tag="ACG8Z3_000127"
     CDS             complement(30240..30884)
                     /locus_tag="ACG8Z3_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0822481.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(30984..31742)
                     /locus_tag="ACG8Z3_000128"
     CDS             complement(30984..31742)
                     /locus_tag="ACG8Z3_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0822482.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(31869..33020)
                     /gene="fucO"
                     /locus_tag="ACG8Z3_000129"
     CDS             complement(31869..33020)
                     /gene="fucO"
                     /locus_tag="ACG8Z3_000129"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0822483.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(33102..33413)
                     /gene="rhaM"
                     /locus_tag="ACG8Z3_000130"
     CDS             complement(33102..33413)
                     /gene="rhaM"
                     /locus_tag="ACG8Z3_000130"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0822484.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(33415..34260)
                     /gene="rhaD"
                     /locus_tag="ACG8Z3_000131"
     CDS             complement(33415..34260)
                     /gene="rhaD"
                     /locus_tag="ACG8Z3_000131"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0822485.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(34274..35530)
                     /gene="rhaA"
                     /locus_tag="ACG8Z3_000132"
     CDS             complement(34274..35530)
                     /gene="rhaA"
                     /locus_tag="ACG8Z3_000132"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0822486.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(35527..36987)
                     /gene="rhaB"
                     /locus_tag="ACG8Z3_000133"
     CDS             complement(35527..36987)
                     /gene="rhaB"
                     /locus_tag="ACG8Z3_000133"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0822487.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            37140..38009
                     /locus_tag="ACG8Z3_000134"
     CDS             37140..38009
                     /locus_tag="ACG8Z3_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0822488.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(38135..39034)
                     /locus_tag="ACG8Z3_000135"
     CDS             complement(38135..39034)
                     /locus_tag="ACG8Z3_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0822489.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(39199..40023)
                     /locus_tag="ACG8Z3_000136"
     CDS             complement(39199..40023)
                     /locus_tag="ACG8Z3_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0822490.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(40034..41329)
                     /locus_tag="ACG8Z3_000137"
     CDS             complement(40034..41329)
                     /locus_tag="ACG8Z3_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0822491.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(41345..41824)
                     /locus_tag="ACG8Z3_000138"
     CDS             complement(41345..41824)
                     /locus_tag="ACG8Z3_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0822492.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(41830..42858)
                     /locus_tag="ACG8Z3_000139"
     CDS             complement(41830..42858)
                     /locus_tag="ACG8Z3_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0822493.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(42875..43981)
                     /locus_tag="ACG8Z3_000140"
     CDS             complement(42875..43981)
                     /locus_tag="ACG8Z3_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0822494.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(44210..46807)
                     /gene="adhE"
                     /locus_tag="ACG8Z3_000141"
                     /gene_synonym="adhC"
     CDS             complement(44210..46807)
                     /gene="adhE"
                     /locus_tag="ACG8Z3_000141"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0822495.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(47112..47492)
                     /gene="yajC"
                     /locus_tag="ACG8Z3_000142"
     CDS             complement(47112..47492)
                     /gene="yajC"
                     /locus_tag="ACG8Z3_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0822496.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(47589..48734)
                     /gene="tgt"
                     /locus_tag="ACG8Z3_000143"
     CDS             complement(47589..48734)
                     /gene="tgt"
                     /locus_tag="ACG8Z3_000143"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0822497.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            48952..49650
                     /locus_tag="ACG8Z3_000144"
     CDS             48952..49650
                     /locus_tag="ACG8Z3_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0822498.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(49818..52457)
                     /locus_tag="ACG8Z3_000145"
     CDS             complement(49818..52457)
                     /locus_tag="ACG8Z3_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0822499.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(53076..54107)
                     /gene="queA"
                     /locus_tag="ACG8Z3_000146"
     CDS             complement(53076..54107)
                     /gene="queA"
                     /locus_tag="ACG8Z3_000146"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0822500.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            54300..55475
                     /locus_tag="ACG8Z3_000147"
     CDS             54300..55475
                     /locus_tag="ACG8Z3_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0822501.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            55479..56120
                     /locus_tag="ACG8Z3_000148"
     CDS             55479..56120
                     /locus_tag="ACG8Z3_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822502.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            56117..57034
                     /locus_tag="ACG8Z3_000149"
     CDS             56117..57034
                     /locus_tag="ACG8Z3_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0822503.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            57031..57702
                     /locus_tag="ACG8Z3_000150"
     CDS             57031..57702
                     /locus_tag="ACG8Z3_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822504.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(58243..58731)
                     /locus_tag="ACG8Z3_000151"
     CDS             complement(58243..58731)
                     /locus_tag="ACG8Z3_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0822505.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(58932..59198)
                     /locus_tag="ACG8Z3_000152"
     CDS             complement(58932..59198)
                     /locus_tag="ACG8Z3_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0822506.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(59198..59461)
                     /locus_tag="ACG8Z3_000153"
     CDS             complement(59198..59461)
                     /locus_tag="ACG8Z3_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0822507.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            59982..60122
                     /locus_tag="ACG8Z3_000154"
                     /pseudo
     CDS             59982..60122
                     /locus_tag="ACG8Z3_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(60264..62087)
                     /locus_tag="ACG8Z3_000155"
     CDS             complement(60264..62087)
                     /locus_tag="ACG8Z3_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0822508.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(62685..65390)
                     /locus_tag="ACG8Z3_000156"
     CDS             complement(62685..65390)
                     /locus_tag="ACG8Z3_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0822509.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(65696..66643)
                     /locus_tag="ACG8Z3_000157"
     CDS             complement(65696..66643)
                     /locus_tag="ACG8Z3_000157"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0822510.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            66832..67101
                     /gene="rpsN"
                     /locus_tag="ACG8Z3_000158"
     CDS             66832..67101
                     /gene="rpsN"
                     /locus_tag="ACG8Z3_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0822511.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(67277..67639)
                     /locus_tag="ACG8Z3_000159"
     CDS             complement(67277..67639)
                     /locus_tag="ACG8Z3_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0822512.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(67650..68771)
                     /gene="alr"
                     /locus_tag="ACG8Z3_000160"
     CDS             complement(67650..68771)
                     /gene="alr"
                     /locus_tag="ACG8Z3_000160"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0822513.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(68787..69137)
                     /gene="acpS"
                     /locus_tag="ACG8Z3_000161"
     CDS             complement(68787..69137)
                     /gene="acpS"
                     /locus_tag="ACG8Z3_000161"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0822514.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(69267..70778)
                     /locus_tag="ACG8Z3_000162"
     CDS             complement(69267..70778)
                     /locus_tag="ACG8Z3_000162"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0822515.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(71100..72452)
                     /gene="murF"
                     /locus_tag="ACG8Z3_000163"
     CDS             complement(71100..72452)
                     /gene="murF"
                     /locus_tag="ACG8Z3_000163"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0822516.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(72605..73681)
                     /locus_tag="ACG8Z3_000164"
     CDS             complement(72605..73681)
                     /locus_tag="ACG8Z3_000164"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0822517.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            73877..75622
                     /locus_tag="ACG8Z3_000165"
     CDS             73877..75622
                     /locus_tag="ACG8Z3_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0822518.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(75902..76339)
                     /locus_tag="ACG8Z3_000166"
     CDS             complement(75902..76339)
                     /locus_tag="ACG8Z3_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0822519.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(76323..77696)
                     /locus_tag="ACG8Z3_000167"
     CDS             complement(76323..77696)
                     /locus_tag="ACG8Z3_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822520.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(78018..78506)
                     /locus_tag="ACG8Z3_000168"
     CDS             complement(78018..78506)
                     /locus_tag="ACG8Z3_000168"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0822521.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(78528..79130)
                     /locus_tag="ACG8Z3_000169"
     CDS             complement(78528..79130)
                     /locus_tag="ACG8Z3_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0822522.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22691 a        16795 c        14460 g        25227 t
ORIGIN      
        1 atattcttta ggatattaaa attaactagc accacgggta gctattaagt aaagcatcaa
       61 aataattgat agcaactttg gtatcatcat tagaatctat ataccttttc aaaaacattt
      121 taaaaaactc atcagataaa ttgtgggttt catttggagc aaacaaaaaa gctaaaaaat
      181 tactatgtct tatttcattt ttagatagct taagtatctg aaatatattt gttttcctag
      241 tccatttaga tagtttttct tcaatatcat ccatatcctc caatagattt tttaaacact
      301 ttttttcatc tatcatgttt tttctccttg ccattattct aactaaaatt ttacgtattt
      361 gtttctatgt ctacattatc tttttaacag tatctatttt tcttttgcaa cttacttggc
      421 taccaaagca aataaaaaag ttatttataa atttctccct accactacct attgctatta
      481 tttaaaatag ggataatttt ttctcttaaa aaatatcatt atttgtggta tggagccccc
      541 gcgttgatcc ggaaggcacg gtagatttgc tcgacgagga tcaggcgcat caattgatgt
      601 ggatacgtca ttttgccaaa agagatctga gcattgcttc ttttcatgac ggcttcgctg
      661 agtcctaaag aaccgccgat aacaaaaaca aggtggcttt ttccttggat acctaattgg
      721 tcgatttgtt tagcaaaaga ttcgcttgtg ggatttttcc cttcaattgc taagccatag
      781 acgtattctt gttctttgat tttcgctaag atccgttctc cttctttttc ttttacttgt
      841 ctcatttgcg cttcgcttaa attctccggg gctttttcgt caggaacttc gatcaattcg
      901 attttcgcat aggcattcag ccgtttgaca tactcattga taccttggat caagtatttt
      961 tcttttaatt ttcctactga aataattttg atattcatgt tttctctcct ttgatcttca
     1021 cgctcacttt gtttattata atcaattttt tcgtctatat gtttattttg actatttttg
     1081 ttaaaaagat ttccccaaca gttatgcaca acatccacag gtttatccac agaaaacagc
     1141 gttgttttac cttatttcat cacattcatt aggcaaacat acgttttata agaatttatc
     1201 cactttatac acaggctgtg gatatgtttt atcccttatt tttccaatcc tatccgcgta
     1261 gttttacaca atatctgtgg aaaaagaaaa tcaaatattt tcatctgaaa acaatgattt
     1321 ttcctaaagt ttttcataaa aagttcaaag tctcaaagta gactaaagtc aacaaataag
     1381 actatactaa cagtagataa atgaatgtaa ttggaggagg aacacatgga tagaaaaaat
     1441 gtgacaccaa agatgaaaaa aaataaaaat aatagcctct ggcgtaagtt gggtctgggg
     1501 cttgttggag ggatcgttgg aggactactg acagctggca ttttttatgc agcgatgggc
     1561 actggcaaca atagttctac tgctacaagc ggcaatcaaa attctgccgg tgaaacagtt
     1621 gtggaaaatg tcaaagtcaa tgtagattct gatatcacca aagcggtaga taaagttcaa
     1681 gacgctgtcg tttctgttat caacttacaa agtcaaaacc aatcaagcgg ttttggcgga
     1741 ttattcgggc agcaagaaga aagtagcagc tcagatgata gtaatttgga agcttatagt
     1801 gaaggaagcg gcgtaatcta caaaaaagac gggaatactg cttatgtagt cacaaacaat
     1861 cacgtagtag atggacagca aggtttggaa gtcatgatga aagacggtac gaaggtaaaa
     1921 gctgaattag ttggaacaga tgcctattca gatttagcgg tgttgaagat caattcagac
     1981 aaagtcgaaa cggttgcctc ttttggggac tcaagtgctt tgaaggttgg tgaacctgcg
     2041 attgcgattg gttccccatt aggatctgaa tatgcgaatt ctgtcacttc aggaatcatt
     2101 tcttccttga accgacaagt aacaagtacg aatgaatcga acgaaacagt caatatcaat
     2161 gcgatccaaa cagatgcagc gatcaacccg ggaaattctg gtggtccttt agtgaatatc
     2221 gaaggacaag ttatcggtat caactcaagt aagatcgcaa gtacttctga atcgacttct
     2281 aatgtaagcg tagaaggaat gggatttgcc atcccaagta acgatgtagt gaatatcatc
     2341 aaccaactgg aaaaagacgg aaaagtgaca cgtccagctc taggtatcac aatggtcgac
     2401 ttatctgccg tttctactca gcaacaagaa caaatcttga agattccttc atctgtaact
     2461 aacggtgtca tcgtgacaaa tgtccaaaca gctacaccgg cagaaaaagc tgggttgaaa
     2521 caatacgacg tcattactaa gatcgatgac aaagaagttt cttctggtgt agaacttcaa
     2581 tctgctttgt ataagaaaaa agtgggagat tctgtcaaaa tcacttacta tgaaggctcg
     2641 aaagaaaaaa caactactgt tgaattgacg atcgatcaat cggcattaaa acaaaatcaa
     2701 aatagtgaaa attaataaaa aagaggttgt gacaaagact tgtcacaacc tctttatttt
     2761 ggaaatactt ctccgactac ggcatcctca ggcaatacta agattccttt tttttctgga
     2821 gcatttggca aatgcaattc acgagcagaa caaatcattc catagctggc tacccctcgc
     2881 aaagttcctg gccagatcat cattccatct ggcatcatgg ctcctggttt tgcaacgaca
     2941 actttttgtc cagcttcgat atttggtgca ccgcaaacaa tttgcaatac tgtcccattg
     3001 tccacttctg tctcagtaat cgataaatgg tcagagtcag gatgcgggac gcatgttttt
     3061 acatacccga caacgatctt cggttcatga tcagcttcaa gctgttcttc gaatcctgct
     3121 tctttaatca atcgattcaa tttcgctaat tcttcatctg ttaagcttac ttggccgttc
     3181 ccttcaattg ttagatgatc ggagatatgg aaaaagttcc aagcaacaac ttgaccttgt
     3241 tcatctttga tccgagcaac attccattta cgttcgacag tgttttctgt tcctttatcg
     3301 tcggctatga taaccatcaa agtgtctccg acgtgtgctt tgttataagc aaaaatcatg
     3361 ttctttcctc cattatcctt tgctgagatt tatcttaaca aaaaattgca ttcactgcat
     3421 gagatttttt tcatgcttta tttccgaaga agcttcttct ggaacaccat ttattcgtaa
     3481 caagggactg tgacaagact tttgtcacag tccctttact gtttgattaa aaaggttata
     3541 caagttccac ttgcttaatt acttggctgt tcttcttcgt cagaaacgat caagacatca
     3601 caaggtgaat gacgtatgac gtagctggct acacttcctg tcataaagcg ttcgactgca
     3661 tttagtccag actgcccaac catgattaga tcgattgcat atttttttgg caatttgact
     3721 gccatggatt cacgaacaga gccgtatgca ataattcctt ctatatttgt gtaatcaaca
     3781 gattttccat attctttaca ttcttcgatc atttgcttgg ctgtttcagt ttgctgatcg
     3841 actagatttt gatcaatagg agcatagccc ataaaattat aaaattgctg atcgattacg
     3901 tttgctacat atactttccc attattacgg cgagccactt ctaacgcttt tttgaaagca
     3961 gcaagtgctt gttcactacc gtcaatacct actaaaatgt tcttatattc ttgattcatc
     4021 ttgacccctc ctaacagatg tcagcctttt attacacttt ctaggaacga ttcgatttcc
     4081 tgttttgttt tacgatcttt attcactaga cgacctaatt cttttccgtc ttggatcacg
     4141 acaaaacttg ggataccaaa aatgttccat tcagcggcta catctatgta cttgtcacgg
     4201 tctacttcaa taaattgcca atcagaaaag tcattttcga tttcaggcat ttgcggtttg
     4261 ataaaacggc agtctccgca ccagccggca gtaaagaaaa atacactttt tccagtttct
     4321 acgtagcctg ctaattcttc taaactattt gggataatca ttctttttcc tctactttct
     4381 gtttatgtat ttttcaatta aattatacac ctttctttcg aactaacaaa cattgatcct
     4441 ctttttataa aaaaaatctg acaaaaggtt gcttttgcca gattttatag tttcactatc
     4501 gcgttatttc ccgtatacga tcgtgttcac tgttgattta tctaaacctt tcaatgtttg
     4561 aatcaacatt tctcgtgcag cttcaaaatc gcgaatactg aacatcgttt gatgcgtatg
     4621 gatataacgt cccactacgc caatcactgt acttgggatt ccttcatttt gtgtatgagc
     4681 agcacctgca tctgtaccgc cttttgaaac aaagtattga taagggatgt tgtttgtttc
     4741 agcgatatct agcaagtact cacgcaaacg aggaagcatg atcaaacctg gatcttggat
     4801 acgcatcaat gtaccttctc ctaaatgtcc gaatgtgcca tttttcgtca ctgtgtcatc
     4861 tgctgcagag cagtctactg caaagaacag atctggcttg aatttattga cagaagcttt
     4921 agaaccacgc aagcctactt cttcttggac attcgcgcct gcaatcaatg tatgacctaa
     4981 tgtttcattt tgtaacgctt ctaaagcttc taataccatt gtacagccat agcggttgtc
     5041 ccacgatttg ctgatgatat tcttcccgtt ggctgttttg atcgtttcag cgtaaggaac
     5101 gattgtgtct cctggaagaa caccgtaact cattgcctct tctcttgatt caaacccagc
     5161 atcgaacaaa atatctgtta cttctactga tttttgtccg cttgttcctc gtaataaatg
     5221 tgggggaacg gaagaagaaa tacatggata attcttacca gtagaggttt taagtgtaaa
     5281 acgttgagca gaaacaacgt aagggttcca tccgcctaga ggtacgactt taaaaagccc
     5341 gttttcttgg atttgtgtca gcatgaatcc gacttcatcc atatgtgctg cgaccattac
     5401 acgaggtgca tccgcttctt catggtggcg cagcccaaaa atgccgccca aaccatctaa
     5461 ctgtaactca tctaccagcg gttccatatg actttccata taggcgcgaa cgtcttgttc
     5521 aaaaccgctt gttccttgta attctgttaa ctctttgatt cgttgaaatg ttttttcttc
     5581 catatgctat tcgtctaaat ttcagactaa actcctctca gacttatatt gatctgcgga
     5641 taaaatatga ttttttctgc agaccgatcg tatttattat agcttatttt ttctggtagc
     5701 ggttatttat ttcaaagaaa atatgagagt gtggtaaaat aaacacaaag agtagcaagt
     5761 gagaagaagg aggtatatcc acatgtatga agaaagcgaa ttaagctatt ttaaaggcgg
     5821 gttagccgtt gggatcggtc taggattatt gagtggggta gcttcaacga tttggtatca
     5881 tcgcaaaaaa gtgttatcag cagaccaagt gcttgaaaat atcaaaacat cttttttaaa
     5941 agaaggcgca atcgaaggtg cttggatctc ttttgaaaaa gaacctattc ggaaattcgc
     6001 cgttcggtcg agtgcttatc ggggtgggct ttcccggata gaagatggcg aaactgttta
     6061 ttatgaattt ttagcagatg ctcatacagg aaccgtatta gacatttcaa gaaaaaaggg
     6121 cgcaatagat taatacgtca aaaaaatgaa ataacgaata cgcacccact atctaaaaga
     6181 gaggtatcct atccttcttt tatagtgggt gatttttata aaaatagggc tatttcctct
     6241 tggaatagct agtatttgta gttattatac ggtattattg aaataagatt agttttatta
     6301 tataaggaga gtgacaaatg gaatcacgtg aaaaaattgc cgtgctcatt ccttgttata
     6361 acgaagaggc gacaatttca actgttattg aagattttaa aagggaatta ccagaagcag
     6421 atatttatgt ctatgataat aattcgaccg atcgaacata cgaattggcc gttcaaggcg
     6481 gagcaatcgt aaagaaagaa ccacgtcaag gaaaagggaa tgtaatccgt caaatgtttt
     6541 ttgatattga tgccgattat tatttgatgg tagatggtga tgatacttat cctgcagaag
     6601 ctgcacatgg tttgcttgag aaattacgtt ctggcgaagc agatatggtc attggcgatc
     6661 gtctatcaaa tggaacctat tttgatgaaa ataagcgtcc tttccatgat tttgggaata
     6721 atctggttaa aaatacaatt acttacttat ataaaacaaa aattcgcgat gtcatgaccg
     6781 gctatcgagg attcaatcga atttttgtca aaagcttccc tattatgagt tctggttttc
     6841 aaatcgaaac agaattgacg attcatgctt tagataaaaa attcaagttc gttgagattc
     6901 caatcgatta tcgcgatcgt cctgaaggta gcgaatcaaa attgaataca ttctctgacg
     6961 gattcaaagt gatcatgatg atcatcaaaa tgtgcaaaga ttataaacct ttgcttttct
     7021 ttgggatctg gaccatcttc ttctttttat ttggattggt catggggatt cctgtgattc
     7081 gtgaatatat gctgacaagc tttattacac gcatcccttc tgcgatttta agtactggat
     7141 tgatgacttt agcccttctt tcattagtaa cagggttgat tttggatacg gtcgtcacaa
     7201 atgccaagaa agaatatgaa ttgaatttat accatgtcta ccacgaatat caaaataaag
     7261 aaatgaagta ggaggccttt tttatgcata agaaaaagga gaatctattt cctacttttt
     7321 ttgcaacttt atttgcgtca ggttttgctt tttttacttc ttatggtcta ggaactttga
     7381 atccaacagt aatcattggt ttagccatgg ttttgctact tgcttttaag caatatggaa
     7441 tagagttgtt ttcaataaaa aacaaagttg cagctattgc ccgattgttt ttttctgcag
     7501 cttttgctct tacactttct attggcagac agatacaatt tcagtatgtc tcagcaaact
     7561 atacaaagac aacgattgct gatggaagta ttttttactt tcttgtttat ttctccattt
     7621 ttgtgtattc ttttttattt ttacaatcga tttatttatc aatgccaaaa ctggaaacat
     7681 tttttactgc tcaaaacaca caggctttct cttcaagaaa atatttttta ttctttactg
     7741 tctttttagt tctgtgttgg cttccttatc tgttggcatt gtatcctgga gtggttttgc
     7801 ctgattcgtt aagttcggtc gcccaatcta tgggtgatgt tcctataagc aatcatcatc
     7861 cagtcttatt caccttactg gtcaagctat ttgttcatga tttaccaact gatacgatca
     7921 atcacggtgt atttttattt tcgatttttc aaacattgat aacagcttca gcgataagtt
     7981 attctctttg ctggctgtta aaaagaaaca tccatttttt tccggtactc attagtttat
     8041 gctattttgc atttgcaccg gtttttccaa tctacgcctt gaacatgcaa aaagatactt
     8101 tgttctctat cgcttgcttt ttattttcct tagcggtttt tgactttatt tttcaacgag
     8161 aaaagccaac gattctcaat cagatttacg taatcatcct tgctttactg accacctatc
     8221 taagaaataa tgggatttat gtagtaaccg gcacaggttt gatttgcggc ttgattattt
     8281 tgaaacaaaa aaggtatcaa gcattctttc aagtggtgct gggttataca gtgattgctg
     8341 gactactcat ccacccgttg atggctcgtt atgctattcc tacagaaact gctgaacaat
     8401 taggtgttcc tttgcagcaa atttctagaa ctattgttat ggatggtgac ttgtctaaga
     8461 aagataaagc attcatgaat cagctactcc cgataaaaga ttatgaagcg tattctccta
     8521 gtcttgcaga ctcaatcaag tggcataaaa attttaacaa tgagtttttg aatacacata
     8581 aaaaagaatt tctatcacta tgggcaagaa atttaccaag taatataaaa atctatacag
     8641 atgcttatat tttagaaacg ttcgggttct gggtacctgg tacaaaaaat agttacggct
     8701 ttttagatac tcgtgtaaac acgaataatt atggtatgaa gcaagtagat ttgattgaac
     8761 atcttactgg attgtcagga atgaaaaaaa tcatcgatgc tcgtgatttc tttggttccg
     8821 gcacattact gtggttagct atcggcagtg cacttttagc aatctttaag aaaaaatatc
     8881 ggtatttact cctttttctt cccggtatgc ttgtttttgc tactattatg attgctactc
     8941 ctgtggcttt tagtttacgg tatgtgtttg ttttagcatt aggtctacct gtttatatcc
     9001 tctctccttt tttactagat aaaagagaaa aaatgaaaac agatatagga gatactcatg
     9061 aaactattta aaaagatatt ttttgctttt gttctcttga tcgctagcta ttgcgtacta
     9121 ctgacaatta cttttttcat tccgcagcac tctattgaat cgaatgcaga aaaatctcta
     9181 cagatggttg aaaaagaagg aatgtatcct tctatcaatc aaggaaatat gctaggcact
     9241 cggttagata attttactga tcatttgatg atacgaaaaa caaaagcaga tcctgaattg
     9301 aactccttgg agaacgcgat gtctatggcg gattatcccc gttattggca tggttatcag
     9361 ctttttttac gacctttact gctagggatg agtttgggga gtattcgaat gatttacgca
     9421 gccattcttt ttctgctgat tggattgacg agctactttt taaccaagtg tagcgacatc
     9481 tatttaacga cagccttgtt gatttcgctt gctataggaa atgctgcaac ctttttcttc
     9541 agtatgcaat tttcaaatat ttggattttg actttattgt ctgtattact atttttaaca
     9601 aaaccacaac tctccaacac caaccaacaa ttattcttgt atttctttgt cattggttct
     9661 ttagctaact tttttgactt gttgacaaca ccggtcatct cttgggggat acctgtgatc
     9721 attatttatt atgtaacaaa taagtattta atggataaac gcagttcatt gagcaaacaa
     9781 gtaggcagtt ttgtttttac cggtatcttt tggggattag gctatggttt gacatgggtg
     9841 gctaaatgga tcctatcatc gatcatattg aacaaaaaca ttgtaaaaga tgcaatcaac
     9901 caaatcctct ttcgaacgga aggaaacgac aaatacccgt tgcatcgatt agagatgctg
     9961 aaaagcaata ttagattgat gtatccaaaa gtagcaatat tattgctatt gattacttgt
    10021 cttgtttttc tctattttgc ttatcgtaaa cgaaatagct ggcgtccgtt ccaacttcta
    10081 gttttgtttg ttttagctgc tgtaacgccg tatatttggt acaatatttt agctaatcac
    10141 tcacagattc attattggtt tacgtatcgt acgcaaatca tcacaagttt tgctatactt
    10201 agtgctttcg cctttttgat ccctcctgag aaatcaaaag acgaactaaa ttacttttaa
    10261 gaataccgat gtactaattc atttttaaac ataaaaactc cagctgtcag agatagccgg
    10321 agttttttag tatttttatt tcgccaaacg atagatggca tctgcataga ttgctgttgc
    10381 tctgaacaag tcatcaagac tcataaattc gtttgcttga tgcattgtat cagtatatcc
    10441 tgggaacatc gctccataag caacgccacg tttcaacaaa cgtccgtaag ttccgccacc
    10501 gatgatttgt tcgtagcctt tttctccagt gtggtcttca tagacttgca ataatgtagc
    10561 caccaatgga tcatccattg gtacgtaatg tggttccatt acgcgagcac ctcgagtaac
    10621 tgttgcgcct tcttgcccca ctgtttgttc gatgtcactt tgcaatccgt ctactgttac
    10681 accttttgga aaacggaaat tcatattgat gaagttcgct tcctctgtac cattttcttc
    10741 aaaagcaaat agtccagcat tcattgtcaa tttgcccatt ttttcatctt cataagcgac
    10801 tcctagtttt tctccataaa aatcttcatg gacataagca gctgctacat ggataaattg
    10861 tttagcagat cctaggaatt catagttatc taagaacaat gccaagaaag aacctgcatt
    10921 gataccagat tgtgggcttg caccatgtgc gcctttacca actaattcga tttttacata
    10981 cgtattgtcc gcttcaatcg ttccgcttac tggatttgct tcgatgaatt ggtaaaatgc
    11041 ttcttccatc gcgatagctg catctgcact tggtacttga agtgctgcag ttgctgttcc
    11101 tggcaccata ttttcgcgaa gtccggaaac aaaagatttc aagacataat ctcctgcatt
    11161 gtcgcctttg aagcgtaaag ctaaagaaac atttcctttt tcaccattga tgattgggaa
    11221 ttcagcatct ggtgagaaac caaaatctgg ttcttcttca tgttcgaaat aataagccat
    11281 atcgccccag ccgctttctt catcgctgcc gactacgaag cgaacttttt ttgatagttt
    11341 caagtcaagt tctttaatga tcttcatcgc atagtaagct gccatacttg gacctttgtc
    11401 atcgcttgat ccgcgtgcaa agatctttcc atccttgatt actggttcat aaggatctgt
    11461 gtcccagcca tctcctgcag gaacgacatc catgtgtcca aatatcccta gcgtttcgtc
    11521 tccttcgcca agatcaatat gccctgcata gttatcgaca ttttttactt taaatccatc
    11581 acgttcgcca taagctaaca tatgtttcaa tgcgtcgcga ggtccaggtc caaatggtgc
    11641 atctggtgtt acttttgaat catcgcgctc gctgttgaca cgtaaaagat ttttcaagtc
    11701 ttcaagcaag tcttctttac gtgcttctac ttctttttgc caatcaattg tcataaagtt
    11761 aatcctccac ttatttaagt cttcgtgttc atgataccac aaattacaga aaaaaatagt
    11821 tcaaaaaatc tgacaattcc ttttcttatc tgtttttctc taaaaaaaat atacgctata
    11881 atgagtaaaa ggaggttttt cgatgctgat aaacgaacaa ctagtgaaaa gagtcatcac
    11941 acctcgtcca aagatttcac ataaaggaac atttggacgt atcgttttga ttggcggcaa
    12001 tgaacaatat ggcggagcaa tcattatgag tgctttagct gccgtacaca gtggagcggg
    12061 tctcacgact gtagtaactg atgaaaaaaa tcggagtgct cttcacagtc atctgccaga
    12121 agcaatgatt gtagattgga cggatactga ccagtttctt tccgtaataa agcaagcaga
    12181 tgtactcttg attggtcccg gattgggtac ttcttctaag agtttggaac gtttcaaaca
    12241 agtactagaa aaacaatcaa aagaacaatg gctggtgatt gatggctctg ctttaagttt
    12301 acttgttgct aatcatcttt tgctacccta tcccgaacaa actgtactta cacctcatca
    12361 aatggagtgg caacggttga gttctctacc gatacaagaa caacgggata aagaaaacaa
    12421 aaagaagcaa caggaaatcg gtgctgtgct cgttttgaaa agtcatgaga caaaaattta
    12481 tggtcaccag cacttctaca ccaatccttt aggttctgct gctatggcaa caggcggcat
    12541 gggtgacact ttagctggga tgatcacagg atttttagcc cagtttcctc aaaatgacca
    12601 aacgattgca gcagctgttt atcttcatag tttcattgga gaccagctgg caaaagaaca
    12661 atatattgtt ttgccgacac aaatcagcga agcacttccg aagtggatga attatttttg
    12721 taaaaaatca atggaaaacg aggcatgagt catggagaaa tttacaggtt atgtgcgaga
    12781 acctttttat tatgaaacag atcaaatggg gattattcat cacagcaatt atatccgctg
    12841 gtttgaagag gctcgggtag ccttattggt acatctaggg tttccctata aaaaaatcga
    12901 agaatctggg atcatcatcc ccgttctgga agtaaattgc agctataaag aaatgatcca
    12961 ttacggagaa aaagtgagca tccacccaac tatccaaaag tatacgggaa cacggttgga
    13021 ttttagctac gaaattgtgg gaactgaaga caaaaaatta aaaactactg gcagtagtaa
    13081 acactgtttt ttactttctg attcgcaacg attagtgaag ctgagcaaag tcaatccgga
    13141 gttagaacag ttattccaag cgtacgcttc tttggatgat gtaaattctt aataaatacc
    13201 gtctaagcat ttttcttgta ttgtgtcgag tagttcgtat aatgaaaaga aaaaagcagg
    13261 tgatccccat gtatgtcgtt aaagtaatgc acggttacat cgataaaact ggctgtcgca
    13321 ctcgggaaaa aaacttagac aatctgctca ttttcaaaga caaaaaagaa tccgaagctt
    13381 ttgctaaaag aatcggcggt cgtgtcaaac cgatccaaga ggtacgacca gattgattca
    13441 atatttttct tttcgaggct ctaataggca tgcctatcag ggcttttttt gcagactgtg
    13501 tttttatgcc cacacgattt gtaagcgatt tatgttataa tagattatat ctgcatagaa
    13561 aggaaggtta aatggttatc aataatttgc aacagcaagc ttatgaatct atacgtaaac
    13621 aaatcattta ttccgagtta gcacctggaa gtaaaatctc cgataaagtg ttagaagaac
    13681 aactagctat cgggcgtaca ccgattcgag aagcactgat ccaactgcgc aatcaagagc
    13741 ttgtaaaaac cattccacag tccggcactt atgtttcaaa aatcgatatc cgatctgctt
    13801 cattagcccg ttatacgcgg gaaaagttgg aaaaaccgat cctgcaagaa tgcagtgcga
    13861 aaatgaccaa gcaagatcaa gctaaactag aaaaaatact agaacaaacc gatcaggcag
    13921 tgatcgctca aaataaaaaa gctttttttg atttggatac tgattttcac cgaacttgtt
    13981 atgaaattgc tggaaaacga gaaatttggg attggctaga aagttatagt acgcacttga
    14041 accgtttcag atggttacgt ttgacgattt cagaattaga ctggggtcga gtattagatg
    14101 aacatcagac aatgcttcaa tcaatgattg atcataactt tgatgaagtc ggatttcttt
    14161 gcacgatgca tttacatatg attatcgagg agcaagaata cgtgattcat aattaccccg
    14221 attattttga agatattcag gacagaccca tcaaataaga ggttttggaa tcagcgtttt
    14281 gacctgagta ttagagaaat aaacggcaaa atagttcctc atattttgcc gttttttttg
    14341 agctaatacc gagggtcagc tgattgcaca ccgtttattg agaggttgtg aaaaagctgc
    14401 cctgcatcaa gtaataagaa cagccaagta aaaatagctt cttatgtttt taacttggct
    14461 gttcttatta ccgcagatgc aagcttttga acaccgtttt ccaagaggtc gtgagcctgc
    14521 cgttcatctc cgagtcacaa ggaacaattt caaaaaacag cttctcatgt ttgttgaata
    14581 ttttgacttg tgaccgagga gttgacagga gaacatcgtt tattcggaaa aaaaggactg
    14641 tgacacgact tttgtcacag tctctttatt tgtaatctaa atcatactcc gtacatcttt
    14701 tttctatttt ttttatttct tctaagatcc cttggctacg aatttcatta tatggccaaa
    14761 gtaagactgc ctctaataca taaggcgaaa aatcgctttg attcatctct tttgaaaaac
    14821 tcatcgctgc tatctcgctc aatgcacgaa tgtttgattg atgtgtataa aaaggcaacc
    14881 gccacagcac aattttttcg ctttgcgtat aaattccctt aacagaagct tcgatgtggt
    14941 gaaatagttc atgtccaata atcaaagctt gcgctgtttc tttagagatc gaagctagtc
    15001 ctgaacattt taatttttct aacggctccg tcatcaatcg aatcccttca tcttctgcat
    15061 aagttgcaaa agtgattcgt tcgtttattt gctctgattc acaggaaacg attggaacag
    15121 ccatttgttc acaaatttct cctacatcgg caaaagggta cttttttttg atgctacatg
    15181 ccgtttcgat accacaatcg attgattgtt gtataagaat tttctgcttg gacaaaggaa
    15241 tctttttcca aaataaatct cgccgaaact ggtagaatcc ccaaatttct tcggggatct
    15301 gttgtaattc gtttagtaac tcttctaact tatgtttgtt tgatatttcc ggcaacgacg
    15361 atcacttctt cctccggttc catagtagat acttccagat ccatcaaaag ctgtagttgt
    15421 tccagatccg tcgctgtaaa atcagagact cttgctccta agcagagata aaattctggt
    15481 cgggcaatca attcagtttg ataaactgcc atatcttccc aaagttgttc gacagctgtc
    15541 atataattgg ctgcagttgt tttcatacat gaagcatgtc cacgttggac cttgataaat
    15601 cctttttgat cgatgatccg aagacttcca gcattttttg aacgattctg ttcgccgcat
    15661 acataaaaga aaggtgtatt ttccaataag caaacttcat ttttatttaa tcgcatatcc
    15721 tctgcagcaa gctctagtgc ttcttctgtt gtgatctcct tggttagatc ggttgctttc
    15781 acttccgtag aaccagtagc gatagccgtt acttttgaag tctgtggatc gatttctacg
    15841 tggacttcga tcgattctgg tgtagcgcct gattcaatcg ctttattcat cgcttcattt
    15901 ttcaacgatc gtatatcttc tttacttggc gaagggatga tacgttctac aacatctcgg
    15961 accattgcaa gtgctacacc aatagaagaa atcacttcgg cgttttcagg aatcgaatat
    16021 ttcacaccca ttttattact aaagtaagta atcaatgagg cagcgccgcc ccctacacct
    16081 acaagagaaa tctgatcttt ctccaagtga tacttgtctg ccaattctaa aatcaccggt
    16141 tcaatcttag cataggattt ttccatgatc tgttctgcga tgtcttcaac cgtcgtatga
    16201 caataatcag ctaatgcttg gatggctttt cgtgcagatg ggacatttcc ataagaaaaa
    16261 tgttcttctt gcactaatcc aaggacattg gctgcacatg tatttgtaat agtcacttct
    16321 tctccgtctt ccatcactac cttgacgtaa tcagcagggt ctccttcttt aggagagaaa
    16381 aattcaacct taggaccttt gattttttct gtctccgtaa atactgcata atctagaccg
    16441 gcaatatgtg cagaacgtgg accgacatcg atgattcctg actgattagc ccgtaccatc
    16501 gaaccgcctg ccacacctag aacccgaaca tctaatgaag agatatatgt tgggtggcca
    16561 cctacaattg aataatcgat tgctggacgt ccatttttaa tgaccccgat attggttgtt
    16621 gttccgccta cttcaaaata aacaccatta gacgctctca agtacatcaa agagcccatg
    16681 actgatgctg ctggaccgga aagcatggtt aaaacaggtc gttttttcat ttcattgatt
    16741 tccattactc cgccgtctcc gcgcataatc atcaaagaaa cattgacccc tgcttctcgt
    16801 acggaatctt ctgtagaggt tgccgtatct agcatttttg gtaaaataga tgcattgatt
    16861 gctgctgtcc gagttcttct tgtcaatcca tataattttg tgatgtcaga agccatcgtt
    16921 gtagggatcg attttaccga tgctgcttca taaacaactc gttctggttc cccattatct
    16981 actccaaatg ccatagaaga aaccaagacc tccgctcttt ctctttctaa agaagaaatc
    17041 gtttctgaaa cacggtctac atttaagtgt ttgacaggta agaatgcatt gacaatctct
    17101 atttttttct tattccctaa atcgatatcg ttcaatctag tttgtcgttt tgctaaaaaa
    17161 ccttctaatc caccttttgc catcccgatt acgccgactt ttgctacgtc tccttcaatc
    17221 agtgcattag tcgcttgtgt ggtagaatgt gcaacaaaca caacatcttc tggactgata
    17281 ttattttccc gcaaacaatt ttgaaaagac tgaacgacac ctgcggctac tccacggaca
    17341 tcatcgtggg ttgttttcac tgaagatttt ccgatgattt cgtgcgtcgc attatcgatt
    17401 gcgactgctt tcgtatgtgt accgccaaca tcaattccca ttcgcacaaa acgtttcatt
    17461 ttcttttcct ccattcatct aatcgttttt atcgacgttt tgtttactag gcgttcccgt
    17521 acaataagaa cgtgatcagg cataagatcg ctccgatgat ccaaccagtt gggatggatt
    17581 tcttcatata ttctcgagaa gttacttttg tataacctaa tccccaagca acccaagatt
    17641 gtgtaatatc taaatgctga ggagcaatcg ttgtgattgc aaataacggg aacaaaaagg
    17701 ctggcgacat cgttggatta acagctaata cgacagctaa aatagctgag cctgaaccga
    17761 ctagattcat tggaccgcag aaaaatccta gtggtgtcaa tatagcaaat acgattgcca
    17821 agactaatgg tgattgcggt atccaatctc caaaaatagc tgaaaaataa ggagaagcat
    17881 acgcagctga attattgaac atcgctaagg tcatcaagaa accaaccatt ggtgcaacat
    17941 ctactgcgcc atctgtaaat aatttcgcaa actgacgaca gatttcttta taacctccgc
    18001 gcaattttcc agtaaacagc aatgcccaga tacctgctag aataaaacct aagatgattg
    18061 gaagatctaa aagaacgacc cctaaaactg gtaataatac ggctaaccat gagatcattg
    18121 gtgcgtcgcc accttcagct ggtacgtttg ctgccattgc atagcgtttc tttttgttca
    18181 ttgatatatt agcgaccgtt agtaccacaa ctaagcttac aatcatccca atcataccaa
    18241 tttggaaata gtcattataa gtatagcttt cagccgctgg attgaaacca gcataaatcg
    18301 tttgatattg tttgaaattg acgatattag caaagattcc agccataatc gagcccataa
    18361 atgagaacag tgcaaccggt actgaaatac ctaatgacat caagattggc aacacaataa
    18421 ctgcaataga aatcactggt ccgattcctg tcatcgacat gaatataaca gcggttacaa
    18481 tacatagtaa gctcatcgtg attcttggct tgtcccctcc aagttcaact acctttcgaa
    18541 tcagggtagc tgcgattccg ctatctacta gaacacgccc gaaaaaggca ccaaagaaga
    18601 catttaccaa gatcgatttg gcgtactcag ctggccccgt cgtatagata tgcgtcaaca
    18661 catcaattac tccttgccct tccatcgccg ggtttggtga aaacgtgttt ccgactagcg
    18721 ctaatcccat ccatacagta gccatgatgg caaagccgac catcaagttg tagcctttta
    18781 cacagtacca gaccatgaaa aagaatgaga taactaacaa aataccaata ataatatcac
    18841 tcatgttatt cctccgtcca ttttgttttt ctgataagca aactaatata tcagtcaaac
    18901 tcattctaac ttatgtagtt agcgctttca ttaaagggtg accataaatg ttcagttttc
    18961 aattaaaaaa ccttaattat gctattttag acttatacgt tattgtcctg tttcatttga
    19021 tggaggattg taaataaaaa aagaggaaag aacataactt ttcgttatct tctttcctct
    19081 ttataattgt ttagcttatt tagccaaagc ttcttttgct tggtcagcca atgcagtaaa
    19141 tgctgctgca tcgttaacag ccaagtcagc taacatttta cggttgatgt cgatttcagc
    19201 aactttcaaa ccgtgcatta attttgagta gctcaagcca ttcatacgag ctgctgcgtt
    19261 gatacgagca atccacaatt tacggaagtc gcgtttcttt tgacgacgat cgcgatatgc
    19321 gtagttgtat gaattcatta cttgttcttt tgctgtttta aataaagtgt gttttgaacc
    19381 gtagtaacct tttgctaatt ttagcactct tttacgacgt ttacgagtta ctgttccacc
    19441 tttaacacgt gccatgtata attcctccta ataaaaataa atcttcagat tctgttattt
    19501 gtacttctta tttcattttt gctagttgtt ggcgaatacg tttgaagtcg ccactagaaa
    19561 ccatgcttgc tttacgcaat tgacggcgtt gtttttttgt tttgccgtgg aaacggtgac
    19621 ttgtaaacgc gcggaagcgt ttcaatccgc ctttaccagt acgtttgaaa cgttttgctg
    19681 atccgcggtg tgttttttgt tttggcatga ctatatttcc tcctcaaaat ctttcgttat
    19741 ccgactatcg gctgtccaac agccagttag tcattcaaat tacttactgt cgtttttcgg
    19801 tgccagcgtc aagaacatgc tgcgtccgtc cattttcgct ttttgttcca ctgttgcaat
    19861 atccgcagtt tcttcagcta agcgattcaa gactttttga ccaatttctt tatgggtaat
    19921 ggcacggcct ttgaaacgga tagaagcttt cactttatcg cccttttcca agaacttacg
    19981 tgcattacga agttttgtat tgaagtcatt caagtcgatc gtaggactta agcgaacttc
    20041 ttttacattg atcacttttt gttttttacg cgcttcgcgg tctttctttt gttgttcgaa
    20101 acggtattta ccgtagtcca tgattcgcgc aactggtggt ttcgcaccag gggcaactaa
    20161 cacaagatcc aagtttgctt gttctgcaat attcaatgct tctgcttttg tcttaactcc
    20221 taattgctca ccatctgaac cgatcaatcg taactctcgt gcacgaatgc cgtcgttaac
    20281 catcatatcc tttgctatgg tcattcacct ccaaatatta tgagagaaag aaaagcttac
    20341 cgaataaacg gcaataaaaa aacgagttct cttacagaac ccgcgcgtac ttcacctaat
    20401 agaaccagtg aaaactatct agcccagtga cattatcaac taaggcgaga agcgggtgct
    20461 tctgctttta tttctcaacc atcatagtat agcaaaaact aatctttttg tcaatgtttt
    20521 tcatacagtt tgtcttcctt ttctgaaaat gattagatta gcagactttc tagcagtgat
    20581 tcccgcttct ttgctatact aaagaaaaac gaggtgaacc cacatgaaaa aaatcgaacc
    20641 tattccacct tctcttcctc ttcttacaaa gggagagttt tatttagaag atgaggagat
    20701 cttctctggt cttctgatca acgggggtga ccaaagctat ctcgatgcca aaaatatggt
    20761 attaagagag agtcatatcc aaaaacttac gatgcaaaaa acagttttac gtcattttga
    20821 atgcagtaat gtcatttttg aaaaatgtga tttttccaat ctggagtgga ttggtggcag
    20881 tttccaccaa gtcgtgtttc atcaatgtaa attaacaggt acgaattttg ccgaaagtta
    20941 tcttcgagac tgtactttca ctgattgtat tgcttcgatg gcttccttta gtagtacaaa
    21001 cttgaaagct gtctccttcg atcattgtca attagaagac agcgaatttt atgaggtcac
    21061 ttggaaaaac ctttttttgt ctgacaatca gttgaccggt tcgaactggt ttcgaacttc
    21121 tctgaaggga ttagacttta cgactaattt cttcacagca atcgctttat ctcaagattc
    21181 tttggcggga ctaatcgtca accaagagca agcacttgtg atcgcccaag cccttggttt
    21241 gcagatcaaa gactagatca acgaatccgc cgccacagtg caataagcat ccaaatagat
    21301 aagaccaaac tgatgatcaa ggcgattccc catccgaaaa cggaatgggt aaagggtaac
    21361 tggagattca ttccgaagaa tcctgtcaca atagttggaa cggtcaagat cagcgaccag
    21421 accgtcaaaa atttcatcgt gtcattcaag ttgttattca gcaaattatt ataagtgcca
    21481 gagagctggt ctaaaatctg cgaagctaag ttcgtcattt caactgcttg tcgtgcttcg
    21541 atcagcgcat cgtccaactc ttctttttcc ttttctgaca acttgcgata aatagccaat
    21601 gccttgattt gttctaaaag tactacattt tgttttgttc ctgtaactaa gtaaaccaaa
    21661 ccgatctcta aatctgaaag ttgcagcaga ttcttgttcg ttgttttttc tcttaatttc
    21721 agattaagcc gctgacgttc actattcact tcctcgatta aaggaaaaaa ggaatcagaa
    21781 atcaaaaaca atgtacgaaa caataaactg tatacactca tgtctggcat tcgttccaaa
    21841 agcgatcgaa ttagtcgaat cacgtactcg gtattcggcg tggtaaatgt aaacaaaccg
    21901 tcttctttta aaataaaagc gataggacta gtttcataat gattatcgac tttttgctgg
    21961 tgcggaacat ttgatacaag cagtaacgct tcatcaaaag aatcgtactc tacgcgcgcc
    22021 cgttcatttt tatctagaga ataagtcagc atctcgtcac taatgccata ttcttctttt
    22081 aagtttttaa cagctagcac atcgttttgc tgtacattga tccaaaaatt acggtcttga
    22141 ttaaaatagt gtttttcccg catactctat cctcctgaca tttgtttcca gtataaagaa
    22201 aaaagtcgct caaataaagg tttacccttt atttgagcaa ctgtaaatca aatggctact
    22261 ttggtttggc gaatctctgc taatttttct tcgatcattt tcaagacttc ttcttttgct
    22321 tgaggatctt ccacaaaatt atagcggtct ccatcgattt ggatttttgg actttctttg
    22381 tattcttcat accattgatc gtaacgtctg ttcaattctt tgtaataatc gtatagactt
    22441 ggatcaaagg acaattgttc atacgaacga ccacgttttt cgatacgctc caacatcgta
    22501 tcaaaagata cacgaatatg aactaataaa tccggatgtt ttttatgggc agcataggga
    22561 agttcttcca tcatgttttc cagcagttca tcatacacac gaacttctgt ttctgttgct
    22621 cttcctagat ctgcatttag atggaaaagc agtgaatctt cataaatcga acggtccaat
    22681 acattattgt catctttcat tgcttgtttg atgcttgcaa aacgtttatt caaaaaataa
    22741 atttgcagca aaaaagcata ctgttcaggg tttgcataaa ataagggcaa gacttcatta
    22801 tcatcaatcg attcataaaa tgcctgacta tcaaagtgtt cagccatcat ttctgttaaa
    22861 cttgattttc cggcaccaat tgtacctgct aaaacgatca cgctcattta cctccagctc
    22921 acttcatcac taaatattgt gtttaactcc aaccttttta aatatacccc caatatctag
    22981 tagtgtcaat tgtatttttt tctagtcaaa gaagaaaaaa agtcaaaaaa ttcttaagaa
    23041 aaaagtgttt tttctttaga aaacgtattc ttagttttac ttctgagatg gttttagtaa
    23101 aataaaggag gaatgttttt tgagaccctt agtggacaaa aaaattcgtg aagcagcgca
    23161 actcgttttg ggcgctttat tttttgctta aaaatcactt ttacgtgaga aggagttaga
    23221 ttatggcaaa ctatggccaa ggagaatcaa gcggaaagat catattgatg ggcgagcacg
    23281 cggttgttta tggagaacca gcgattgcct ttcctttcta tgcaacaaaa gtcaccgcat
    23341 tccttgaaga gctggatgca atggacgatc aactggtttc ttcctactat tcaggaaatt
    23401 tagccgaagc tcctcatgca ttaaaaaata tcaaaaaatt attcattcac ttaaaaaaac
    23461 agcatgacat ccaaaaaaac ttgcaactga ccattgaaag cacgattcct gctgaacgtg
    23521 gaatgggatc aagcgctgca gtcgccacag cagtcactcg tgctttttat gattacttag
    23581 catttccttt gtctcgtgaa atactattag aaaatgtcca gctttcggaa aaaatcgccc
    23641 acggtaatcc tagtggaatc gatgcagccg ctactagcag cttgcagccg atttatttta
    23701 caaaagggca tcctttcgac tacttttctt tgaacatcga tgcttttttg attgtcgctg
    23761 atacaggaat caaaggacaa acaagagaag ccgtcaaaga tgtcgctcac ctctttgaaa
    23821 cccagcctca tgaaactgga caaatgatcc aaaaattagg atacttgacg aagcaagcaa
    23881 aacaagcaat cattgaaaat tcaccagaaa cgttggcaca gacaatggat gaatcacaat
    23941 cacttctgga aaagctgaca atcagcaatg attttcttaa tctattgatc caaacagcaa
    24001 aagataccgg ggcattgggt gccaaattaa ctggcggtgg acgcggtggc tgtatgattg
    24061 cattagcaca aacaaaaaca aaagcccaag aaatcagtca agcacttgaa gatgcgggtg
    24121 ctgctgaaac ttggatacaa ggattaggag tacataccta tgtttaaagg caaagcacgc
    24181 gcatatacca acattgctct aatcaaatat tggggtaaga aaaatgaaga actcatcctt
    24241 ccaatgaaca acagcctttc gttaacattg gatgcatttt atacagagac agaagtcatc
    24301 ttttctgata gctatatggt agatgaattt tatctagatg gcaccttgca agacgaaaaa
    24361 gcaacaaaaa aagtcagtca gtttcttgac ctttttcgta aagaagccgg cctctcacta
    24421 aaagcttcag taatcagtca gaatttcgtt cctaccgcag ctggattagc ctcctctgcc
    24481 agcgggctag ctgctttagc aggcgcttgc aatactgctc ttaagcttgg attagatgat
    24541 ctctctcttt caagatttgc tcgacgcggg tctggttcag cttgccgaag tattttcggt
    24601 ggtttcgtcg aatgggaaaa aggccatgac gacttaagtt cttacgctaa gccagtccct
    24661 tccgattctt tcgaagacga tttagcaatg gttttcgttt tgatcaacga ccagaaaaaa
    24721 gaagtgtcca gcagaaatgg gatgcgtcgg acagtcgaaa catccagttt ttatcaaggc
    24781 tggttagatt ccgttgaagg ggatctatat caattgaaac aagcaatcaa aacaaaagat
    24841 ttccaacttc tcggagaaac gatggaaaga aacggactaa aaatgcatgg aacgacttta
    24901 gctgcccagc ctccattcac ttactggtct cctgacagct taaaagcgat ggatgcggtg
    24961 cgacaattga gaaagcaagg cattccatgc tactttacga tggatgctgg ccctaatgtc
    25021 aaagttttag tggaaaacag ccatttatct gaggtacaag aaacatttac caaattattt
    25081 agcaaagaac aagtaattac tgcacatgct ggtccaggaa ttgcgattat tgaataaagg
    25141 gagattttta tgattgaagt atctgcacca ggcaaactct atattgccgg agaatatgca
    25201 gttgttgaaa caggccatcc agcagttatc gctgcagtcg atcaattcgt gacagtcact
    25261 gtagaatctg cacgaaaagt cggaagtatc caatctgcac aatatagtgg gatgcctgta
    25321 cgttggacaa gacgcaacgg agaattagtt ttagatattc gggaaaatcc ttttcattat
    25381 atccttgctg ctattcgctt gactgaaaag tatgcacaag aaaaaaacat tcttttatcc
    25441 ttttatgatc tgaaagtaac aagtgagtta gacagttcaa atggccggaa atatggattg
    25501 ggttctagcg gtgccgtgac cgttgcaacg gtcaaagcct tgaatgtttt ttacgcgttg
    25561 aatttatctc agttggagat tttcaagatt gcagcactag ccaatttagc agttcaagat
    25621 aatggttcct gcggcgacat cgctgctagc tgttatggtg gctggatcgc tttctcaacc
    25681 ttcgaccatc cttggctcca agaacaagaa actcagcatt ctatcagtga gttacttgcc
    25741 ctggattggc caggtctatc cattgagcca ttgattgctc ctgaagattt acgtttattg
    25801 attggttgga cgggtagccc tgcctctact tctgatttgg tcgatcaagt tcaccgttcg
    25861 agagaagata aaatggtggc ttatcagctt ttcttaaaaa atagtacaga atgtgtcaac
    25921 gaaatgatca aagggtttaa agaaaataat gtaacgttga tccaacagat gattcgaaaa
    25981 aaccgacaat tactgcatga tttatctgca atcactgggg tcgtcatcga aacgcctgct
    26041 ttgaacaaat tgtgtaattt agctgaacag tatgaaggag ccgcaaaatc ttctggtgca
    26101 ggtgggggcg attgcggaat cgtaattgtt gaccagaaat ctggcattct tcctttaatg
    26161 agtgcatggg aaaaagcaga aatcactcca ctgccgttac atgtctatag cgatcaaaga
    26221 aaggaaaacc gatgaatcga aaagacgaac atgtttcact agctaaagcc tttcatgata
    26281 aacaaaaaaa cgaatttgac ttcgtacgga tcatccataa ccctttgccg caaatagcag
    26341 tttctgatgt cgatctaagt acacaggcag ttgggtttac actaagcagc ccattttata
    26401 tcaatgcgat gactggcgga agcgaaaaaa caaaaaaaat caaccaagat cttgcaatcg
    26461 tcgcaagaga agcagatttg atgattgcca ctggttcagt cagcgctgcc ttgaaagtcc
    26521 cttctttagc cgatacttat acgattatgc gacaagaata tcctcacggg aaaatcattg
    26581 ccaacatcgg tgcagggact tctgtcgaaa gagccaaaga agctatccga ttgtttcacg
    26641 cagatgcctt acagattcat ttgaatgcac ctcaagagtt ggttatgcct gagggagacc
    26701 gtgattttac caattggaaa gtacttattc aagaaactca aacagccatt gacgtccccc
    26761 tcatcgtaaa agaagtcggt tttggcatga caagagaaac gctcaatgat ctagctgctt
    26821 taggagttca tacagttgat atcagcggtc gaagcggaac aagcttcacc cagatcgaaa
    26881 acgcccgtcg ctccaagcgg gaactgagtt atttagctga ctgggggcaa tcgacagtct
    26941 cttcattgct tgaagcaaat gaagcagaca cttctatgga aatcctagcc tccggaggta
    27001 tccgcaatgc ctatgacata tttaaagccc tttgtctcgg cgcaaatgcc gtgggtactt
    27061 caggaaccgt actgacgcat ctgatgaatc acggcgtaga agaaactatt atactgatga
    27121 agcaatggca agaagagctt cgtctgcttt atactatggt cggcgcaacg aataccgcgg
    27181 ccctacacca acaatctctg atcttttccg gaccagtgaa ggattggtgc gaagcaagag
    27241 ggatcgactt agtgaagtat ggaagacgca cagaaaagag cgtgggacaa aagtaaagaa
    27301 cacttttgtc tcacgctcta aacacgtaca aacggcggaa gtaaaagcaa ctccttcgga
    27361 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaattcctt cttatttctt
    27421 ggagttaaat gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc
    27481 cttcggtatt cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc
    27541 tcttttaagg ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc
    27601 cgttaacgaa atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg
    27661 gctcgcccca gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt
    27721 caccgttttc ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc
    27781 caattccatt acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca
    27841 tttttaatcc gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca
    27901 tcatggagca gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg
    27961 cttttgaaac gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg
    28021 catccatttc gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa
    28081 ctgaacctac atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca
    28141 catcgcagac ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat
    28201 caactgattc ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc
    28261 gtgcaatctc aaatccaata ccatagacag caccggtgac taatgctact tttccatcta
    28321 aacgaaacat atccatacta aattccatca ttttctctcc cttatgctta ttttaattga
    28381 tccatttgca ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg
    28441 aatgagtaat tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct
    28501 tgttcattgt ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca
    28561 cgtgtatctt cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta
    28621 tgacaaggca tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat
    28681 tgacagcttt cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc
    28741 aggccttcac cagtctccat tggttttact tggtcgatac tgatttttac atttggatat
    28801 ttatgatgag ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa
    28861 gaaaatacaa catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct
    28921 tctttttgcc catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca
    28981 cgacgctcta agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt
    29041 tcagtaggtg tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt
    29101 ttccctggtt caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa
    29161 tgacggatat cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac
    29221 tccttctatc gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga
    29281 ctaaattggt tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa
    29341 tcaatgattt cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa
    29401 gcatctccgc cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca
    29461 tattcatggt tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta
    29521 cgcttggtcg aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa
    29581 taaattagag gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca
    29641 tccatttttc cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg
    29701 gcttcttgct gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca
    29761 gctttttttg cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct
    29821 ggggtgattc ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat
    29881 tccaaatgat ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg
    29941 cgttcgccac taccagtttc catataataa gtacctagtc ttgatccacc agataatacc
    30001 tgtttcgtat ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta
    30061 tcaggtactt ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg
    30121 tttgcttccc ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc
    30181 tgctctgtgg acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc
    30241 tacctcttga tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca
    30301 aaatctcctg tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca
    30361 gcatcgaacc actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact
    30421 tgaggcatcg gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat
    30481 agtttgacga tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat
    30541 cctggtaagt aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa
    30601 gggctaacaa tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat
    30661 actgtgcctg cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg
    30721 attacttgat ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg
    30781 atgattgcgt ggcaagcatt caaagcttct tctttgcttt ttccgcgtac caccgcaatc
    30841 actccggcgt ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc
    30901 ctatttagaa attttatttt ataacttgga aatctatacc catattatac ggcagaaaag
    30961 aaaaattgca aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt
    31021 tgcttgaggg cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata
    31081 cttacactaa atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa
    31141 atatcacgtt cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgtttggatt
    31201 tcctttttca accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc
    31261 ttttcaagat aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc
    31321 gcagaattgt ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt
    31381 tttggatcta gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata
    31441 gtctcatcga tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg
    31501 atttgttcaa tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc
    31561 tctgaatttt tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta
    31621 gaagaggtca ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct
    31681 aaatagtcta gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc
    31741 atataatatt ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa
    31801 agagatatcc agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg
    31861 cttcgttttt acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt
    31921 ggatttcctg gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct
    31981 tcttttacac ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg
    32041 atctcaccac aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc
    32101 cctagaacaa gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg
    32161 acagttggca aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac
    32221 gggtgagcca tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata
    32281 tactgtccta atgccatttt ttctcgccca ttttgatcgc catctacaga aacttccaaa
    32341 gaatggccga tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct
    32401 tttgtgataa acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat
    32461 tttttcggca ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga
    32521 ggatcaacac aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt
    32581 acttctgctg ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc
    32641 gtatcagcaa caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc
    32701 ccaatggctt ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc
    32761 tctgcctctt taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg
    32821 atcccgtcgt ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt
    32881 gctataccgt gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc
    32941 tgctgaaatt cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt
    33001 aaaatcattc gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa
    33061 acgaaagaac cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc
    33121 atcaattccc aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac
    33181 ttccaccatt tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg
    33241 atcactaagt aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg
    33301 accccgtgtt cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg
    33361 tattcttctt gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac
    33421 acctgctttc gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc
    33481 tgctaaatgc cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac
    33541 gtacgtatag acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat
    33601 gtcttttcct gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat
    33661 tttttcagct gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga
    33721 aattccttct ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa
    33781 ggaacgttcg tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg
    33841 cataacgatc cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact
    33901 tggcaactcg cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt
    33961 gccatcttca gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt
    34021 aaaatatctt cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa
    34081 aatcatcgag atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc
    34141 ttttagtaaa taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt
    34201 cgacgtcgtt tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa
    34261 tatagccaca ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt
    34321 ccagccaatc caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg
    34381 catacgggaa atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca
    34441 gctccatttc tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg
    34501 tatttctcgt accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca
    34561 gaccgatatt tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca
    34621 attcatcatc catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca
    34681 acatgattcc tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg
    34741 gatgaaaatg tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat
    34801 aaaattcatg ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta
    34861 ctgcatcttg tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca
    34921 ttaaacgttc ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc
    34981 aaaagttatt gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc
    35041 ttcttttccc gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa
    35101 agccgtcttt catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt
    35161 ctttttttgc ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga
    35221 cagattcttc tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca
    35281 acgaataagc cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa
    35341 aattccctgt cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga
    35401 catcatctcc ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag
    35461 catctgtatc tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa
    35521 tcgtgttcat acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt
    35581 attttcatct gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg
    35641 ttctatattc gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt
    35701 ggcttcacta ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag
    35761 tagacgatta ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa
    35821 gtgttctact ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg
    35881 agcgatagct cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat
    35941 ctcttctatc atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg
    36001 cagcggtttt tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg
    36061 cattcgggca acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt
    36121 gttatgggca ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc
    36181 gatctttgtt tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc
    36241 tccttgacct ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa
    36301 ggtcacatct ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt
    36361 tcccggctct gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc
    36421 tatttcccat tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc
    36481 cgtcaccatg ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg
    36541 tgcttttgcc aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg
    36601 gatctgaatc cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc
    36661 tactgctcca ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg
    36721 ttcatcaatc agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat
    36781 ccctgtctgc tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt
    36841 ccagcgatcg tgaccgtctt tgttccgaaa gccgtttttg aaacggtgta cttcttccaa
    36901 gatcaaagaa ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat
    36961 atcgatggca acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc
    37021 actttattaa taaagcgtta tcatttatta acaatgctat cataatacag aatcttatat
    37081 tttgcttcac actattttgg tatataattg cattatcttg acaagttggg agtgtttaca
    37141 tggattactg ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga
    37201 cgcctacaca aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag
    37261 caccaatccc ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt
    37321 tgtctggcaa aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact
    37381 tattttttat tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg
    37441 aaatggattg gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa
    37501 aagacggcca taacaccttc cattcaacag gggatccatc agaaaacaag atccaccatt
    37561 tgatcacaga attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct
    37621 ctgtagctat ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg
    37681 aagaagagga tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca
    37741 aagaaacgat tacacttgat caattagaac aacaattctt attgaataaa tatcagctga
    37801 gcaaagattt ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac
    37861 tttcttacgc taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag
    37921 aaatagggat tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat
    37981 cgccgagaga ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa
    38041 tattttgaaa tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac
    38101 agcagctact gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat
    38161 aatgaaccaa ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact
    38221 ctctcttgaa tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag
    38281 cactagagct gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta
    38341 catactgatg aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc
    38401 tgacaaaaaa ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga
    38461 tatattgtaa catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac
    38521 tctcttcttt ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat
    38581 attttatgtc ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt
    38641 gcttcaatac ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa
    38701 aacaagccga tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa
    38761 agatatagac acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg
    38821 aatggatgag cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag
    38881 tcgcttctcc ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt
    38941 tattttggct gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat
    39001 tgacttgttc accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta
    39061 tgcacaattt tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt
    39121 ataaaaaaga agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta
    39181 accaacgtct tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt
    39241 tgtttcttca agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt
    39301 tgtgtactcg atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg
    39361 aaaatctttc aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac
    39421 caattggact tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat
    39481 tcctagatag tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac
    39541 cggatgattc aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac
    39601 caagcgacgg ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt
    39661 atattggatt tccggcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga
    39721 agaagcatag cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc
    39781 tggatgaatc atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa
    39841 aactcgtttg acataatttc ccatcccaga attgatctca tttgcgccag aaggtgtttc
    39901 aggaaacgaa agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc
    39961 tgcaagatct tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa
    40021 cataactatt ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag
    40081 taatcaaaaa caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg
    40141 cctctgcttt tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg
    40201 tgatggagcc tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc
    40261 ctacacttgt gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat
    40321 ccataaacat acccaccagt aataacacaa gattgactaa taacagaata ataaatttat
    40381 tatccgttac gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg
    40441 cgaaagacat catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg
    40501 attgaaacaa catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa
    40561 tcaacaggga gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca
    40621 agataccacc gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt
    40681 ttccagcatg agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga
    40741 tgaatgctac tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa
    40801 acaaagcaga gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg
    40861 gaggaatgac catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt
    40921 catatccttc ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa
    40981 tggccgagct agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag
    41041 cccccggcac gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc
    41101 cattattcat gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat
    41161 tgatacttcc caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta
    41221 acaataaagt catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa
    41281 gcaacacaac aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc
    41341 atttttatat tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca
    41401 ggctatagat aatgatgagt acccccgata caggtactga cagataaaca tatcccatag
    41461 gaatacttaa agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat
    41521 acaccatgat catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt
    41581 tacttttttt gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc
    41641 ccacaacata cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt
    41701 ccgtgaccgt atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag
    41761 cgaccatccc aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt
    41821 tcatcacagc tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc
    41881 gtttttgaat atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc
    41941 attgaattgg actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc
    42001 aaaagcttca atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg
    42061 ctcatcagtt agctgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt
    42121 atgctcatca taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa
    42181 ttcattgttt tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact
    42241 tcccataggt acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg
    42301 catcacacgg atcttctttc cctccaaatc ttccggcgta tggatcggac cgtctaccat
    42361 ataaaagctg cgctgaccag aatcataata ggtcaatccg gtaaatccta aatttttggt
    42421 cgattggtag attggctcca ttatttcttc attatccatc accttaaaga aatgttcttc
    42481 atcattgaat agataaggaa ctgaaaaaat cgaataatct ttagagaaac tttccaacgc
    42541 tgaggcactt acttttgtga aatctactgc tcctgactgt gtcaattcga tcagttcacg
    42601 ttctccaccc agttgtccat ctgggaaata ctggatctgt acggaaccat ctgttttttc
    42661 ttttactaaa cgcccaaatt cattcattgc atcaattact ggctggctgt tgcttgcata
    42721 agcaaagcgc aaggtaacta tctcatttac atctgcatct gcgacagttg ccacactaga
    42781 attatttccg cagccgctta gacctagcag acctaggcta agcgaacttg ctgcaaacag
    42841 cagtgatgtt tttttcatat gggtttcctt tcttttaaca ataagagacg agcaattctg
    42901 tcaaacaaag caccgttaac gactgtccat aaggcattgc tgtgattccg atattccgat
    42961 aaaaatctaa actgtccccc attcctgtcc cgattgaaac gttttgaact tctccttttt
    43021 catcgatttg ttcaagcaat ccttggatag cacgataagc gacttcttta tattcttgtg
    43081 gtaaataacg tttatggacc gctttcaaaa taccgtaagc aaatcctgca gttgcagaag
    43141 attctacgta agaagaagga tcatccaaca acgtatgcca taaaccgctt tcagactgat
    43201 aagtttttaa tgcacgcact tgtgcttcaa gtgtttcgat caataatttg cgcaatgcat
    43261 cttctttcga taattctaag atttcaatga tctctggaat agcaatcgtc aaccagcagt
    43321 ttccacgagc ccaaaatgct tcggcatagt tatgatttcc ttcaaacgtc cagccgtgat
    43381 accacaatcc tgttcgttta tccatcaagt atttgatatg gatcataaac tggtaacgag
    43441 cttcttctaa atattctggg cgattcaaca gtttcccgat ttttgccaaa ggaagcactg
    43501 tcatcatcaa tgtatcgtcc cacaactgat tcttattttc cggtccatat gtggcatgct
    43561 gtaacccgtc ttcatttgtc cgaggcatgt cattcatgac ccattctgcc cattgttcta
    43621 aataaggcaa atagcgtgaa tctttcgtgt cttcatacaa gtaagccata gtcaatagcg
    43681 gtgccattgt attgacgttt ttgggcggag cgccttcttc catacgatcc tcaaaccatt
    43741 cattgaccac ttgataagcg cgaggatttt ttgtcatctg gtaattttta taaataccat
    43801 atagaccgac accttgtggc caattccaca ctgtccagct tttgtcatct actttcagtc
    43861 catcaaagtc caacaaaaat tcaccattat cgtctttgat cgttgtcagg ttttcaacga
    43921 gcaggtcaat tttttctttc actaattctt taggtacaaa aatctcttct ttaataatca
    43981 tctttacgcc acctttttaa acgctttcat tttatatcct taatatagca gtaataccgt
    44041 ccactataaa tatcgctttt tgaacaaaaa ctgtctaaaa ttgacctaaa tgttaaataa
    44101 caaaagtaac tttcagttac ttggaaatta aaaaaagaac ttggagaaac aacgttttcc
    44161 aagttctttt tcatctattt tcttatcttt tttctacgct ttcttttgct tatccgttgt
    44221 aagcatcaat gatgatctgt ttcaattcag agatcaatgg ttctttcgga ttagctgtcg
    44281 tacattggtc ttcgtaagct aattcagcca tacggtctac ggttgtatct agtgtttctt
    44341 gtgtgactcc ttgagctttt agactcatgt tgatgccgac agattttcct aattcataaa
    44401 cagccgttgc taaagcttcg actaattcct ctgttgtttc gcctttcaga cctaagaatt
    44461 ttgcgatatc tgcgtaatct gtatctgcac ggaaatagtc atatttaggg aacatcgcat
    44521 gtttttgtgg atcttttgca ttatagcgga taatatgcgg taaaagaatc gcatttgtcc
    44581 gtccatgagg gatcccgtat tccccgccga ttttatgtgc gactgagtga caaataccta
    44641 ggaatgcgtt agcaaacgcc ataccagcca ttgtagaagc gttgtgcatt ttttcacgtg
    44701 attccatatc aggtgttttt actgattttt ctaaatagtc aaagactaac ttgattgctt
    44761 gtaggctcaa tccacgtgta taatctgaag ccatgactga aacataggat tcgattgcat
    44821 gtgttaacac atccatcccg gtatctgctg tgacagatgc tggaactgac atgacgaatt
    44881 gaggatcgac gattgcgaca tctggcgtca atgcataatc tgctaacgga tatttcacat
    44941 gtgtttcact gtcagtgatg accgcaaatg gcgttacttc agaaccagtc cctgatgttg
    45001 tcgggataca tacgaattgt gttttttctg gtttgtcgat cttatatgtc cgtttacgga
    45061 tatccaagaa tttttgtttt gcaccaaaga aagatgtgtc tgggtgttca tagaacatcc
    45121 acattccctt agctgcgtcc attgcagaac cgcctccaag agcgattact gtatctggct
    45181 tgaagtctgc catcagtttc gttcctgcgt atacggtatt tgtcgatggg ttcggttcaa
    45241 catctgagaa gacttcgatc ttcacatcat ttttacgtcg tgccaatact tcacgaactg
    45301 tatcgcagta accgaattgg accataccag ggtcacaaac gatcatgaca cgttcaacgt
    45361 tttccatttt ctctaagtac aatagagagt tcttttcaaa gaagattttt ggcggtaatt
    45421 tgaaccattg catgttattt ctccgtttcg ctacagtttt gacattgatt aagtttacag
    45481 cagagacgtt acgagaaaca gagttcttac cataagaacc gcatcccaaa gtcaatgacg
    45541 gaatcatttc gttatagata tcaccgatgc cgccttctgc agatggtgta ttcactaaaa
    45601 tacgacaagc tttcattcga agaccgaatt tgacatgcaa ctcttcgttt tctgaatgga
    45661 taactgctgt atgtcctaat cctccaagat tcaacatacc ttcacacaaa tcaaacgcat
    45721 ggtccgtgtt gttagctttg atcatggcaa gaactggaga aagtttttct cttgatagcg
    45781 gataatctgg gcctacgcct tccaattcag caatcagcat tttcgttcct tttggtacac
    45841 tgattcctgc caattttgcg atttccatcg cagaatgtcc aacgattgat ggattgaccg
    45901 catattttcc ttcgttcatc actgcatctt ctaatttttg caattcatct ggttttacga
    45961 tataaacttg gtgtgcttgg aattctgctt ttactgctgc atagatttct ttatccacga
    46021 tcacagcttg ttcagaagca caaatcattc cattatcaaa tgttttcgaa acgatcaaat
    46081 cgttcactgc acgtttaatt tttgctgttt tttcaatata agctggtacg ttacctggac
    46141 ctacacctaa agctggtttt cctgtagagt aagcagactt gaccattcca gctccacctg
    46201 tcgctaagac gatcgcgata cctggatggt tcattagcat agaagtcgct tcgatcgaag
    46261 gatgttcgat ccattggata caattttcag gagctccggc agcgatagca gcatcacgta
    46321 ccactcgtgc tgcttctgca gaagattttt gggcacttgg atggaaagca aaaacgattg
    46381 gatttcttgt tttcaacgcg atcattgatt tgaaaatcgt tgtggaagtc ggattcgttg
    46441 ttggagttac tccgcaaacc acaccgactg gttcagcaat ttcaatcaac cctttttgga
    46501 catcttcatt gatcacacca actgttttat catgtttgat attatgccaa atatattcgg
    46561 acgcatacat atttttgatt gctttatctt cgtaaacacc gcggcctgtt tcctctacag
    46621 ccatcttcgc taatggcata tgttgatcca aagctgccat tgccatttca tgcacgatat
    46681 ggtctacttt ttcttgatca aagctttcca tttctttcaa tgctgcattt gctttttctg
    46741 ccaaatcatc aatcattgct tgtacgtcaa ttgtcttcgt tttttctttt tctattactt
    46801 tagccatcat tatcctccta atgggtcatg gttatttgtt aaataattca catgtatatc
    46861 atacaatatt tatttttcta tgtaaacact tttttgtgat ttttttcaca gttattttta
    46921 aatataaaat tgctttattt acaggtgttt gtatagaaaa atatataaag ttatttatta
    46981 ttatttatat gacacacccg attattcaat attgagcaat atatcccatg ctcttatctc
    47041 tttctttttt gaataaaaaa tgaggtagga catctttctg ccctacctcg tctttaaata
    47101 agctttgata cttatttttc ttccttctct tctgtctctt ccagcgtttc tgttgtttca
    47161 gtttttactt caactgtctc agctggttct tctgttgttt caacagtagt cgttgcatca
    47221 tttgttacga cagtccctgg tttgaccgtt ttgattgctg cacgatcgta ctcaaggaag
    47281 atcccttcgc agtctaaaac aactgtccgt ttgtcattgt cgatttctga aacaactcca
    47341 tgtaatccgc cgatcgttac gactgaatca cctggtttca tgctatctaa caagttttga
    47401 cgttcttgct gttgtttctt ttgcgaacgt gacataaaaa accacattgc taatagcgcg
    47461 ataaacatca caagcattgg taatgttccc atttttttca cctcactaat tctgtatcta
    47521 caaaaaacac tttagcagaa aaatgctgat aacactagtt ttttacttaa tttttaaaag
    47581 attttccttt agaagctttt tgcgttttct ttattgaacc cgtattcttc aaaaaaggct
    47641 tctctaaatt caagcaaatt atcatccatg attgcttgac gtacttgttt cattagattc
    47701 aacaagaagt ataaattgtg gtaagaagtc aaacgaatcc caaatgtttc atcacatttg
    47761 atcaaatggc ggatataggc acgtgtatag tttttacatg tataacaatc acatttctca
    47821 tcaattggac ggaaatcatg ggcatatttc gcatttttca ctactagacg gccttgagaa
    47881 gtcatacatg tgccatttcg agcgattcga gtaggcaaga cacagtcaaa catgtctact
    47941 ccacggataa caccatcgat caatgaatca gcagcaccga cacccatcaa gtaacgtggt
    48001 ttgttttcgg ggatcaacgg tgtagtgaat tctaatacac gattcatttc tgatttaggt
    48061 tccccgacag ataacccgcc gattgagtaa ccagggaaat ccatcccgac tagatctttc
    48121 gcgctttgac gacgaagatc ttcaaaccct gctccttgga tgattccaaa taatccttga
    48181 cggtctggat tagcatgagc tttcaatcct cgttctgccc atcgagacgt acgttctact
    48241 gattttttca cgtagtcgta actttcatcg aatggtggac attcgtcaaa actcatcatg
    48301 atatcagagc ccaatttatt ttggatgttg attgcttttt ctggtgataa aaacatacgc
    48361 gaaccgttca agtgattttt gaaatgcacg ccttcttctt cgatattccg catatcactt
    48421 aatgaaaaaa cttgaaagcc gcctgaatct gttaaaatcg gttgatccca gttcataaat
    48481 ttatgcagtc cgccagcttc ttctactaag tcttcaccag ggcgtagcca taaatgatat
    48541 gtattactta aaataacgcc agcacccatt tctttcaact cttctgggga cattgttttt
    48601 actgtagcta atgttcctac tggcatgaac attggtgtgg ggaaagttcc gtgtggcgtg
    48661 atcaattccc ctaaacgcgc gccagtatgt ttttctttct tgattaaacg atagcgaatg
    48721 gcaggttgcg tcattcattt ccctactttc tatataaaat atcttgacaa ctcccaccat
    48781 cataatcgtt tcaagtgctt tttgcaagtt ccctcggctt ccttctactt tatttaatat
    48841 tctttttttt atcctttttt tatgaataac aaaaaaaaga aaaacattat gacagaaata
    48901 ttttttgata catggtatat tggtgttatt aaagaaaagg agggaaaaat catgcaatct
    48961 tctgcagaat taaaacgcca agcaaaagag agtctaaaag gacgttgggg acaagctgtt
    49021 ttactaaatc taatccctac attgattggt attgctacta ttcttcttat tatgattcct
    49081 attatcttgt ttgccaccac catatatgat ggatcagcga gcgtctcaga agcgacaact
    49141 tcaggaggcg gtagttcagg aggcggtggc ggaattattt ccagcatcat tagcgctttg
    49201 ttcatgagcg gtatttcttg gacttatctt gatattttac gtggacaaaa agatcagatc
    49261 gaaccattca aagatgcatt tcgtggattc aaaggtttgt ttatcggcgg agtgatcagc
    49321 ttagctgtgc tgatctcgat ctttacaaca ttatggggtt tgctattagt tattcctgga
    49381 atcattaaaa gctattctta ttctcaaagc tattttattt actacgatat cgtccaagaa
    49441 acaggacaaa cacctaaagt actggatacg atcactgcaa gtcgaaaatt gatggatggt
    49501 tataaaggac aacttttctg gttagatctt tcatttatcg gctggcatat cttagcatta
    49561 gcaactgctg gtattggtta tctatggctc agcccataca tttctgcaac aaaagcagca
    49621 ttttatgaac aattgccaaa agatatttaa aagagagtgt gacttgtgac cgagggggtg
    49681 gcaggagaac accgtttatc gagaggcagt aaaagaaaca ttcagcccat tagcaaaaag
    49741 gaggtgtgac aacattcatg tcacacctcc tttttgtata gttccagtaa aaagaaaatc
    49801 aacttagaaa cagttaatca ttcctcttct tctgcttccg gtaatcgatg gaacttgatc
    49861 agttcattca ccgcgattgg tacgatagat aaaatcccta ccaagaacca gtgattcaaa
    49921 ctgatgtgta ctaggccgaa tagttcttgc gtaaatggca gcaaagcaac agccgttgta
    49981 atagctaatg ccagtaacgc catttctacc aaagctttat tggtcgctaa attgactttg
    50041 aagatcgatt ggctgctgcg tacattgaac acatgcagaa tcgatgaata agctaagatc
    50101 aagaatgcaa cagtttgacc gacatgctga cttgcgtcta cccatgggct gatagtatcg
    50161 acataactac ctaaataata accaaacaag gttacgatgg tgaagacccc tgcatttatg
    50221 gcaatttttt gcaataaacc tcgagcaaag atcccttcat ttttcggaat gggtggctga
    50281 tccatgatgc cgtgttccgc tggctctctt cctaaagcaa atcctggaag tccatcagca
    50341 actacattga caaacagtag ttgcacagct gtaaatggtg ctccccatcc taacagcatc
    50401 gcaatcaaaa caataaagat ctccgaaata ttacagctta gcaaaaagtt gatagcttta
    50461 cgaatattct gataaaccga acgtccttga ctgacagcgt ctacgatcgt cgcaaagttg
    50521 tcatccgtca aaatcatatc tgcggcactt tgcgccacat ctgttcctgt gatccccatg
    50581 gcacacccaa cgttacttgc tttcaacgct ggtgcatcat tcacaccatc cccagtcatc
    50641 gctacgactg cacccgtacg ttgccaagat ttgacgatcc ggatcttgtc ttctggcgtt
    50701 acccgagcat agacagagag agctttgaca cgcgcatcta attcttcgtc tgataattga
    50761 tgcagttctg atcctgaaag tgcttctgac ggatcattca agatacctaa ttctttcgct
    50821 atggcactgg cagtcaccac atgatctcca gtgatcatga ctgttttgat gccagccttt
    50881 ttcgctcgct tgattgctcc tttactttct ggacgtggtg gatcgatcat tccgatcaga
    50941 ccaatcaaac gtaagtcttt ttccagtgct tcagaagaaa tatcttgcgg ttcttcgtca
    51001 taagtagcat aaccaacagc aatcactctc agtgcccgtt taccaaagcg atcattaacg
    51061 atcgccgctt gttctacatc gcctgaacta aagcgcggag caagaacatc gaatgctcct
    51121 ttggtgattg agatgtattt ttcgcccatt tggtgaactg ttgtcatcat ttttcgttca
    51181 gaatcaaaag gaagctcgtt tactcgtgga tatttttctt ctaattctgc ctttgtatga
    51241 taattctctt caacagccct tacgatggct acttctgttg gattaccttt gataacaagg
    51301 tcgtcatctt cttgctcgac cgtcacatct gtacatagtg cagccatttt caatatttcc
    51361 atcgcgctgt tcgtcattga atcttcgata ttcgttactt catcttcttt gctccatacc
    51421 cggcggacgc gcatttcatt ttgtgtcagc gttcctgttt tgtctgagca gataacatta
    51481 gcggttccta gcgtttcaac tgctggcaac tgtcggatga tcgcatgttt cctcgccatc
    51541 ttctgcacac cgtatgcaag tgtcaacgta acaataacag tcaaggtttc cggtacagct
    51601 gcaacagcca aggaaatcga agtcatgaac atctcaagga gtggttctcc ttgcagttca
    51661 ccaatcacaa atactaaagc cgcagccgct aaagcgatca tactgattcg tttgcctagc
    51721 tgattcaatc ggacctgtaa aggtgttttt tgcatagtat tttcgttcaa aagatccgcg
    51781 atcttaccca tttctgtagc catgccaatg gcagtgacga tcgctttccc acgacctgca
    51841 gcaaccgtac atcctttaaa taccatatta agttggtcac ctaatgactc atcttcttca
    51901 gcaataaatg ctgcatcttt ttcaactgct tcactttctc cagtcaacgc cgattcttct
    51961 acacgtaatt gtgatgcttc gatcaaacgg gcatcggcag aaatagcatc accggactct
    52021 aaaacaagaa tatcacccac taccagttgc tctgactcaa cagtggtcac tttgccgtct
    52081 cgtatgacag ttgccgtttg tttattcata tcttgcaagg attccagtgc tttttctgcg
    52141 tttccttctt ggacaatcgc caataccgag ttgatcacaa cgatagcgat gatcagcagt
    52201 ccttcaaata aatccccatg ttctgtagca aatgcggtat aaaaggaaat caccgctgct
    52261 acaagaagga tgatcgttgt aaaatcggac aagcttcgaa agaatttttt gatcatcgat
    52321 tctttcggcg cctcatcgaa tttattcaat ccctgctttt ttcgccgttc atccacttct
    52381 tgtgaagaca acccagtatc tggatctgct tctgttacct tctttacttc ttctattgtc
    52441 tgcttgtatt cttccatcct attcctcctt taaaatcttc gtacaaaaaa aggtacttat
    52501 tttactataa catccttttt ttatttactt caaataaagg gcttatcgat tatttgttaa
    52561 gaaagcgtaa aatttaaagt gcttatattt gcaaacgcta ccaaaggatg ctattatata
    52621 gatgtaagga aggaggatac catcaaatag acagaagaat ttctcgatag aacagtgtaa
    52681 gcaaacaaag ttgttagaag tacaggtttc acccagacat gcgtgtagag tacaaaccta
    52741 taaatagtga atgcgtatgg atgtttggat cacgaagtat ccgaaacaag aagtaatgcg
    52801 atacgtaaca attgaataac gtaaacgtct gaaaattgaa taggaacaag agatgtttca
    52861 aatgttgaag atgtaacgaa aacaaaattt ttgtttaaca tgaagaaatt caacaccgtg
    52921 ctttgcatgt atgatggaaa gttacaatca tttctgttta tttgaatcct tccgaggtac
    52981 aagaaagccg agcttatcga gagctcggct ttttctttgt cataaaatct ttgtaaagct
    53041 tttttgttat tcaaaactag cggcttttca taaccttatt tcacgaacat cgcatctcca
    53101 aaactaaaga aacgatagcg ctcttcaatc gcatgctggt aagcagataa agtcaattct
    53161 cttcctgcaa aggcactaac taacatgact aaagtcgatt ttggcaaatg gaaattagtt
    53221 gagaaagctt ctaccacttt gaattcatag ccaggcgtaa tgaaaatatc agtccatccg
    53281 ctgtctgctt tgatttctcc attaaatttc gttccaatcg tttctaatgt tcgaattgaa
    53341 gttgttccga cagccacgat tttcccgcca tttttacgta cttggttcaa tcgctcagcc
    53401 gcttcttccg tcaaacggta gaattcacta tgcatatggt gttcttcaat tttatctacg
    53461 ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca gatagactaa ttcaacaccc
    53521 tttgcttcga tctcatcaag taattctttc gtaaaatgca atccagctgt tggcgctgca
    53581 gcagaaccat tttctttcgc atagacagtt tgatagcgat ctgggtcttc cagacgttct
    53641 ttgatatatg gaggcaatgg catttctccc aatgattcaa gaatctctag aaaaattcct
    53701 tcatactcaa agtcaatgat ccgtccgccg tgttccaatt cttctttcac tactgcttgc
    53761 aatctgccat caccaaaaga gattcttgtc ccaactttgg cacgtttcgc aggtttgatc
    53821 aatgtctccc acgtatctcc ttgtgtattc gttagaagaa ggacttctaa atgcgcacca
    53881 gtatctggtt tttctccata taaacgagca ggcagaactc gtgtattatt cataactagc
    53941 gcatcaccag gctgtaactc ttcaatgatt tcatggaaat gtttatcttc catctctcca
    54001 gttttccgat ccaaaacaag taaacgagaa tgatcgcggt cttttaatgg tgtttgtgcg
    54061 attaattctt ccggcaagtc aaaatcaaaa tcttctgttg ttaacatatc gtcactcact
    54121 ttctatcatg tgcttcatca ttctatcatg tttttcgttc tgttggcaaa atcagcactt
    54181 tttcacttct attttttgtt ttcctaaata aaaaaactac cctatttgta ttttttcatt
    54241 gttttaggca tactagtaat agatagttct tcattgaaaa tagaaaaaga ggtggcacca
    54301 tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg aaaagtcgct gtagaggacg
    54361 tcaatctttc atttgaaaag ggtgagttca tttgttttat cggtacgagc ggaagcggaa
    54421 aaacgacgac gatgcggatg atcaaccgaa tgactgatcc aacacaaggg aaaattttga
    54481 ttgatggaaa atccatccaa gaaattgatc cagtggaatt gcgacgtcag atcggctatg
    54541 ttatccaaag tattggattg atgcctcaca tgacgatccg tgaaaatatc actcttgtcc
    54601 aaaaattgtt gaaagtcagt caagaagaac ggaataaaac agctgaaaaa atgattgatc
    54661 ttgtggaatt gccccgtgag atgttagatc gctacccaca tgaattatct ggtggtcagc
    54721 agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca agatatcatc ttgatggacg
    54781 agccattcgg tgcgcttgat ccaatcactc gtgattcctt gcaagacctt gtcaaagatt
    54841 tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca tgacatggat gaagcgatta
    54901 aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt gatccaattt gatacaccag
    54961 aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga gttgattgga gaagatcgcc
    55021 tactacaagc caagccaaat gcgacaagag tcggcgaagt tatgctaaat acagcaatca
    55081 cgattacacc ggaaaaatca ttacaagaag caatcaaact gatgagggaa aaacgtgtag
    55141 atacgttgtt agttaccgat aattcgaatg tattaaaagg atttatcgat gtagaaacgt
    55201 tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat tttgaataaa catgtttttt
    55261 acgtaaaaga atccgcctat cttcgtgata ccttgcaacg cattttaaaa cgcgggctga
    55321 aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg tatcctgact cgtgcatcat
    55381 tagtggatat cgtctacgat gtgatttggg gagaagatcc cgcaactact gatgttgcag
    55441 agtcagctac tcctgaagaa tctaaggagg tctgacctat gcagcagttt tttcatcagc
    55501 acggttcaga aattctctca aaaggttttg aacatcttta tatttcagga cttgctttga
    55561 tcattggtat catcattgct gtaccgcttg gtgtcttact gacacgtttt ccaaaagctg
    55621 ccacgattgt gattggctta accagtgcct tgcagacaat cccttctctt gctttattgg
    55681 cattgatgat cccgctattt ggcgtaggta aattacctgc gattatcgca ctctttattt
    55741 attcattatt gccgatttta cgtaatacgt acattgggat gaaaaatgtc gattggaatt
    55801 atcgggatgt ggcaaaaggc atggggatga ctgagttcca atccattttt tccgttgaat
    55861 tgcccattgc tatgccaacg atcatggctg gtattcgttt agctgccgtt tacgtgattg
    55921 catgggctac acttgcctct tatatcggcg ctggtggttt aggcgatttg atttttagtg
    55981 gactgaataa ttaccaacca gatctcattt tggcaggtac gatccctgta acaattttag
    56041 cactattggc tgattggctt ctaggtctct tggaacaccg gctgacaccg atcgcactta
    56101 gagaggagaa tgaagaatga aacgtatact aaaaatgacc atggttcttt tctctcttct
    56161 tatacttgcg agttgttcct tccctggtct tgccagcaat acagatgaag acaccatttc
    56221 gatcacaggt ggaatcactt cagaagcaca gatcttagca agtctagttg ccggaatggt
    56281 tgaacattat acagataaaa ataccgcaat catcaacaat ctggctacta caactatcaa
    56341 ccatcaagcg atgatgaacg gagatgcttc aatttcagcg gctcgctaca caggaacaga
    56401 tttaacgact acattgaacc ttcctcctga aaaagatccg aaaaaagcgt ttgctaccgt
    56461 gaaagatgaa tttgagaaaa gatacggtca aacgtggttc ccgtcctatg gttttgaaaa
    56521 tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa taccatctat ctaaagtcag
    56581 cgatctgaaa aatgtagcgg atgaattagt agctggtgta gatacttctt ggatcaatcg
    56641 taaaggagac ggttatgacg gcttccaaga aacttatggc ttctccttca attcgatatt
    56701 acctatgcag attggacttg tttatgacgc agtcgaagca ggaaaaatgg atattgttct
    56761 tggctactct acggatggac ggatcgccag ttatgatcta gtcatgttgg aagatgatcg
    56821 tcaattcttc ccaccatatg atgctgctcc agttgtagac aacaagatat tagctgatac
    56881 gcctcattta aaagaagcct taaataagtt aggtcatacc atcagtacgg aaaaaatgca
    56941 ggaattgaac tacgaagcag ataataatct agtggaacca tcagtagtag cacaacgttt
    57001 cttacaagaa aaccactatt ttgaagggaa gtgaagcaat tggataaaat gaatgtcttt
    57061 caacaatttt tctattattt ccaggaaaat ggctcctata tctttgcaca atttatccgt
    57121 cacttcctga tttctatcta tggtgtcctc tttgcagcag ttgtcgggat tccggttggg
    57181 atcatgattt ctaggagacg taaattagca aactgggtga ttcgtttggc gaatatcatc
    57241 cagacgatcc cttctctagc catgatctcc attttaatca ttggattagg cctgggagtc
    57301 aatgtcgtga ttgttactgt atttttgtat tccctcttac cgatcatcaa gaatacgtat
    57361 accgggatga tccaagtaga caaaaacatc cttgatgtcg gcaaaggcat ggggatgact
    57421 gctagacaac gactattcat ggtagagtta ccactctctg tttctgttat catggcaggc
    57481 ataagaaacg cgttagttgt tgccatcggg ataaccgcga tcggtgcatt cgtcggtgct
    57541 ggtggtcttg gagatatcat tatccgagga accaatgcaa cagagggcac atcgattatt
    57601 ttagcaggtg cattgccaac tgcattaatg gcgatcatca cggactggct tttaggtatt
    57661 ttggaaagac ggctagatcc agccagccgt acatcacgct gatgttattg taaaaaacaa
    57721 aagcttctgc gaccatttgt tggtcgcaaa ggctttttta gttctgtcat ttttaaggca
    57781 ataatccact ctgattcttc cctccttatg ggcaaagagt tgcttttctt agctatttat
    57841 ccattccgta tactccaact tcatactgcc aacgggttca tatctgcatc caccgactaa
    57901 gtttttttct tatccgctaa ggcattttgc caatccattt tcccatcact cgtcatggaa
    57961 ttcataccac atgatctcta aatactcgtc tgcttgttct ttgaattggg catcaattct
    58021 tacatccttt ggcgtggctt tcatttaagc cttttccatc agtcctcgat tgttcgatgc
    58081 cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt aacttcgatc atcttttata
    58141 caatcgggaa cttgccatcc tcatccttgg ctaaaaacat atctagccat tccagcttct
    58201 cgccatggga ggtttgcggt tcctaaaacc ggtacacgat tcttatggac tgcatccgtc
    58261 gcaactagac ttggcgatac gatgatcctt caccagaaga gcacccccgt aaaccaattg
    58321 atcgatatat tgccaataag aaaagacatt ggcgtaaaag atatcgatcc ctcgtgatgc
    58381 attgccgttg atactgctat tcatggccga aagcgccacg actttaactt cttccgtttg
    58441 agtgacattc atgttcggca atgtcgcttt ggatacccgc tctgctaaag gaacgacact
    58501 ggtattgtat ttcgtatctg tatcttttcc aaaagaccat gccaacgaat cctctgggaa
    58561 ccaataagac gactccggtt gattgtccat tgcttttttc aacatacttt tggctgtttc
    58621 tcgataaacg acatttccaa ttgtcttcgc ctcactttgg cgataatcga tggtcgttgc
    58681 cactgccaac actaacaacg atgctccaat tttactgatt ttccttgtca cacttcttcc
    58741 tccttgtttt acataaaccc gtcaacattc cattcctaaa ctcaaacaat gacccttcaa
    58801 aggtggagat ataggaacaa attaaagaaa tcatgcaata aatccaagaa ggttggtttt
    58861 agaacaaaaa aagagtctcc tttttgggga gactctttgt taactatcta aaaaggttgg
    58921 ctcttctcac ttcactcata atgtgtccag gcagaataaa tttctacaac tttattttcc
    58981 tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc tgcgtgagta tctgccttca
    59041 tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat atggatcttc cttcaatgtc
    59101 tgaatcactt cttcaaattg tttctttaaa ttcgtttgct tgatttttct taaatccact
    59161 ttcgcagagt ttttgattgc gactgtatag ttactcatca aagagcttcc caatcaatat
    59221 catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc tcgtacttta tctaaagtac
    59281 ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca gtcttccagc ccaatcaata
    59341 cagcattgtt tccactacga tcactaataa tctcgatttc agtatgattt tcatttacct
    59401 ctttcaataa ctgataaaaa tttttacgag cagtgctagg attcatcgtt gtattagcca
    59461 ttgtctctcc tcctttttct gatataatta tagcgtactt gattaagtac gtcaaaaaaa
    59521 tacaataaag cacttaaatt cagcaaactt ttcctctttt ttacatagag aaaaagaccc
    59581 ctttttaggg aggatctttg tccacgctca cttcttactc ctttacgact agcggtagcc
    59641 aaacgtaaag taattggtaa caacgtatca tctttagcaa aggtcgaatc caaagcctgt
    59701 gagtctatgc aacaacacca atacacgaag gttcctcgtt gcagtgtgac ttgattgata
    59761 tcttgaataa cctgaggata cgcacgaaag atttgtaacg gttattcaaa actgattttg
    59821 gccgtcaagt cttctactgc ctcaaaaatg attgcctctt tcgtctcctg tcattcatgt
    59881 tcactatcga tttgatactt gcttagaccg ttgggtttgt gaatcaccaa ttaaaaatgg
    59941 cactgagtta tggtaacaaa aaatggtcac ttgacgaggc tgagattcct acttatgaag
    60001 acacatggca cttaagagtc gaggtaaagg aagaagctga aaatatagga ggctgtacat
    60061 tatttggcaa aggattcgga aattatgatc aagacatcaa aatcaaaatg ttttacagct
    60121 aaataggaag aataataaca tttcatacgc tgttcccatc tttaatggct tatagagggc
    60181 acctttagct gagaataaga caaaacagac cctctagctt caattaagag ctagagggtc
    60241 tgtttttgat tttaaaactt ggtttatttc ttcaaatggt agctataggt gctgacaacg
    60301 atattttcac gccaagacaa tcgcaatctt aaccgcaaac ttgtttggtt atggagaata
    60361 ttctgccagc gtctgtttgg tacaaattga ggaatcaaca cagtagttgt atagttttgt
    60421 ttcgctgcat ttttacttac gatatctaca taacggataa ttggattttc aatcgagcga
    60481 taagaagagt ggacgattga aaaacggaca tctgggaaat gctttttaaa ttctgcttcg
    60541 atttcttttt ctttttcgac atcttcgtct agcgaaacat gcatcgcaac gacataatca
    60601 ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc gtgtcacatt cccgactaat
    60661 acaagaacgg tattcccgtc ataatcatgt agttttgcat cattttctaa acgaagctgt
    60721 tctgctactt ttttataatg atcatggatc ttataaaaaa tgaacataac gattggcata
    60781 atgatgaaga aaggccaaat gtcccctaac cgatagacaa acaaaatcgc aatgattgca
    60841 tatgagatga acgcacccgt aatattggca atcgatttgc ttaaccatct ttttccttct
    60901 tttttccatt tgacgaccat ccctgtttga gataaagcaa atggaatgaa cacgccgata
    60961 gagtataacg gaatcaagcg ttctgttgag ccatggaaaa taaacagcaa tacgatcgaa
    61021 cctagcgcta gtgtgataat cccattggaa tagcctagtc gatccccacg atcttgatac
    61081 atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa gaactggaaa agcagaaaaa
    61141 ccggtgttag cagcaactgc taaaatcagc gctgtagcaa attgaaggac gtaatacaaa
    61201 ataccatgtc caaagacagc tttaccaatt tgagacaaaa cagtgacttc tttttcagga
    61261 acgatcccat accaatagtt gataaatgtg atccccacga agaagaaacc taagatcaat
    61321 gccatcatcg tcaatgtagc agcggcattt tttgctcttg gcttcttaaa gaatggtaca
    61381 gcattactaa tcgcctccac cccagttaaa gaggaagaac cagaagaaaa tgctcgtaaa
    61441 atcaaagcga tagaaacacc cggcacagcc gctcctggaa gtgcagtagc ttgcaatggc
    61501 acagcacctg tcacgatatt atataaacca acaacgatca gtaacgagat cacaaggata
    61561 aatgtgtaca ccggaaataa taggaacgat gcagattccc tcagcccacg aagattcagc
    61621 atcatcagta gcaaaacgat tgtcacagaa atagcgactt gatgaccgta gagtgctggc
    61681 accgctgatg tgatcgcttc agcacctgct gagacagata ctgctactgt caacatgtag
    61741 tcgatcaata aagagccacc ggaaatcaag ccagcattct ttccaagatt ctcactgctg
    61801 acgacatatg ctcccccgcc atgaggataa gcatggatga tttgacgata tgatagcgtt
    61861 aatgaaatca ataaaataat aacaaaagcg gcaatgggta gcgaatacca gatggctgca
    61921 gcagacaaag cgactaaaac gacaacgatt tgttccgtac catatgcaat agatgataac
    61981 gcatcagacg acaataatgc caaagcagca aatcgggtta atttgtgctc atcattttca
    62041 gcagatttta aaggcttacc tactaacaac ctttttaagt aatccaattt ctaaactcct
    62101 ttacacacaa ttttacttca agttttatag tcgaaaattt atagtaaaca agatgttata
    62161 gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa ataattggaa aaaaacagaa
    62221 aaatatcaag cgctttttca aacgctttaa tacagcaaaa atctaacatt tttttatggg
    62281 ctttttcttt gaattctata ataaaaggat agaacttgac ttagaaaatc aagttctatc
    62341 ctttttgata tcttaacttt tggaagagag atgtaaggct gctttctacc catcattctt
    62401 actactcgaa gcatagcgca gttgtcacaa cctcttgata aacggtgttt aaaagcttgc
    62461 atctgcggta ataagctcaa ccaaattaaa aacataagaa gctgttttta tttggtcgtt
    62521 cttattactt gatgcagagc agctttttta caacctcttg gtacacggtg ttcaatcagc
    62581 tgaccctcgg tattagctca aaaaaacggc aaaatatgag gaactatttt tccatttttt
    62641 tctctaatac tcaggtcaaa acactgattt cacaacctct ttttttaatg aaaaatatgg
    62701 ctctcatgcg ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc
    62761 gttcctacga ttccaaagac aacaaacaaa ccgatcatga tccagattgc tttcgtagca
    62821 tttggccaat aaattcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc
    62881 aagaacggat tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct
    62941 ccgccagaga tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat
    63001 aatgcagcca agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac
    63061 acgctgtaga ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg
    63121 atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct
    63181 cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt
    63241 gaaagaagaa cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa
    63301 ccgttatttg cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag
    63361 tcactttctt ttgttgcgtc tagtttcaat aatttgatga cttcgcctag atcgacttct
    63421 tgttcgcctt ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag
    63481 tcattgttga tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca
    63541 tttactgttg tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata
    63601 aaatcagcgg ccaatcctaa tttttgctga atgatcggta attcattgtt gtataggtct
    63661 gccccttcat tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca
    63721 tcatggattt cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact
    63781 gcattcgtta cagttgcttt cgttgagctg agaagcgaat caaagtcgat ctggtccgct
    63841 ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga cttggtctag tagagaagtg
    63901 acagtggaac tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca
    63961 acggcatcaa tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct
    64021 gtgttcaaag cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc
    64081 tgatcaataa ttggctgtaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac
    64141 tcgaccagtt tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat
    64201 acattgctga tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt
    64261 acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc caattgactt caatgtaaca
    64321 tctacactgt ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt
    64381 tggtttgcta gatcacttgc ctgattttct aaatttttca catctggcaa gatatcttgt
    64441 acttgttgga tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct
    64501 tggatcgaag caaaatcgcc gtcgatctct gctagttgct taccggcatt ttggatctct
    64561 gggatttttt cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg
    64621 tcattcaatg caacgacttt ttcacccatc tcatctactt tagggatgta atccacgaat
    64681 tcatttgctt tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact
    64741 tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatcttta
    64801 actttattga tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc
    64861 aataaagtcg aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt
    64921 gctcctttat cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg
    64981 atttttggtt tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt
    65041 ggcaagtaaa tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt
    65101 gaatcaacga attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc
    65161 ccaatggcca cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt
    65221 ggcagctctc cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat
    65281 agcgaaggga tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc
    65341 cgtttccagt caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc
    65401 cttcttcttt tttcagctga attactttac catgtatttc atactttttt caatcggctt
    65461 ttttcaaaaa agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac
    65521 atattgagaa ataattaagg attacagcct ttactttatc atgaaataaa agggaaagat
    65581 aattttatgc caaagttttt atctattata cgtatacgca aaaaagctaa aacatttttt
    65641 gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt ttattcctgt atttcttatt
    65701 tttcgaatga caggatgatt ttcccagcag gaggatgtcc ttcaactagc tgatgtgctt
    65761 ggatgacatg cttaagtgaa gcagacagat tcattgaaat aaaaacatga aaggctcctt
    65821 tttggtaggc tttgattatg ccagcaaagg cttctgcatc tagatattcc tttcttggaa
    65881 caaacgattc ataaaagcct tccttttttt gccgcaaatc tagatctggg aggtcattta
    65941 atgcaacata cctgccgcct ggtttcatga tctggatgcc ggtttctcct ttgatacttc
    66001 ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa ttgcagacct ggatttgttt
    66061 tatcataagc agcaaatgct gaagctccta atttttttac cttttcctca tttttactgg
    66121 atgcactggt taagattcgg attccctttt catggagaag ctggatcaga cttgacccga
    66181 ccgcacctga tgctcctagt atcattacag tatctgtcgg ctggatttcc gtcaaatggt
    66241 tgattagatt ataagccgta attcctggcg tgaccattcc ggctgcttct tcccaactca
    66301 ttttatctgg aatttttgct acttttgctg atggcaacac gacttcttct ccgtaagtcc
    66361 cgccgactgc atggacagct actcgatcac ctacatgaac agttgtgaca tctgaaccaa
    66421 tctctgtcac aataccagcg ccatcattac ctggtacata agggaatttc aatgttcgta
    66481 tctctttcat ttctcctaaa cgcaaggcaa catcataagg attcacactg aatgctttta
    66541 tttctacacg aacatgtcca gaaccgactt ctctgggatg agcatcgatt tcttcaaaaa
    66601 catcttgtgc ttctccgtaa ccgttaattg caaatcttcg catggtcacc atctcccttt
    66661 ttctttaagt ttacgtttat gtctttattt tgacaaaaaa atgtcttttc gttttggcgc
    66721 atgattcaaa acaaaatagt tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa
    66781 gcgtaaagat tacgatttac gctaataaaa aataattgga ggaatttgtt gatggctaaa
    66841 aaatcaaaga ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta tgctgcttta
    66901 cgccaggaat taaaagcgca aaaagattac gaaggcttgc gtaaacttcc tttagatgct
    66961 cgtccagaac gattgaaaaa tcgtgattta atcgatggac gccctcgcgg atacatgcgt
    67021 aaattcggga tgtcccgtat aaaattccgt gaattagcac ataaaggttt gattccagga
    67081 gtaaaaaaag ccagctggta atccaagtct ggctgtaatg taaaggagga gggcgcatgg
    67141 accactcctt ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa tgctggtact
    67201 agaagcagac gtatcttttt ttacgcacag ctggtccttt tttatatgta aaggctgcat
    67261 ggttacttcg ctatatttat ttagtaacag gagtcagtgc ttcgataccc acactaattt
    67321 tcaatgctcg gtcaacttct gccataatgg gtcgatccaa atggcagacc ttttctttca
    67381 atcttgattt atcgatcgtt ctgatctgct ccaacaggat aacggagtct ctttctattc
    67441 ctgttccagt agctttgatc ccaatgtgag tcggaagctt aggtttagcc atttttgccg
    67501 ttatggccgc aacaatgatc gtcgggctaa aatggttacc tagattattt tgaataacca
    67561 aaactgggcg tgttccgcct tgctccgatc ccacgactgg agaaagatca gcaaaataaa
    67621 tatctcctct ttttaccata ggatcctcct tattcttgat attctcttgg tatccgctgt
    67681 cctaatccgc aagccacttc atagtggatc gtacctaatt gatctgctat atcctgcata
    67741 gtgatttctt tgtctgcatt tttgccgata agcgtcactt ttgttccgac aggaaattct
    67801 tgaggcaatc ggatcatcag ttgatccata catacacgcc caatcgtttc acagtagttc
    67861 ccttctacca atagagaaaa accctgcatc tttcgtggcc agccatctgc ataaccgatc
    67921 ggtatcgttc cgatccactc tgcttcagga gtgatatatg tttctccata gccgattcct
    67981 tcacctgctg ggagtttttt gacttgaatc aattcagaaa ccagctcaag tgctggctgc
    68041 aatggataaa cttccggtag tgcgtgccca gagggattaa gtccgtacat tgctactcca
    68101 taacgaatca tattgccgat tgtttcatca tgccataaag ccgttgcact attgctgaca
    68161 tgtacatatc gaggaagttc gggaagtttt ttcagcactt ctataaagcg ttctttttgc
    68221 agattccaat atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc
    68281 cattctaata cacgactttc tttgatgaat gcagctgcct cttttacttc ttcaggtgta
    68341 caaaatccga tacgacccat cccagtgtct gcttttagat gaatcgaaag aggcgtctct
    68401 agcttacttt tctctaaaac agcagctgct gcttgcaacc attcccgtgt acctgcagtt
    68461 actgataaat catgagcaac agctacagtc agtgcgtcgt aaggaaccat attcaagata
    68521 aggatgggct gagtaaatcc agcttctctc aactcgactc cttcatctaa atttgaaaca
    68581 caaaagcccg aagctccacc tgcaactgct gcctcagctg tttcaatagc tccatgaccg
    68641 tatccgttcg cttttactac tgcaaacaat tcttttcctt gcggaagtct tgcaacttca
    68701 tttgctacat tttcagttat agctttttta tgtatcactg ctttcgttgg gcggtgccaa
    68761 gcgacaacca tcgtttttcc ttctttctag tcttccaaaa taatttgtgc aaaagctgtt
    68821 tcatttgtat gagtaatact gacccataca ttgccttcat gaggtgaacg cgtcacaaca
    68881 ggctgaccat tttcattttt taaaatctcg acatcttgaa aggtaacttt tccgatccca
    68941 gttccccaag ctttagagaa agcctctttg caagcgtaac gcccgcctaa aaattctact
    69001 tggcgatgaa aaggcaagga ctggaatagt ttcatttcat cagaggtcaa gattcgtgca
    69061 atgaattttg gtttttcttc gatcagccga gtgatcctag gaagttcgac tgcatcgatc
    69121 ccaattcctt ttatcatatg cttttctctt ttcaataaat ttagaacact ctcattctac
    69181 caaattataa ccattttgcc acaaataagt ccacaaataa caaaaatgaa agtttctctt
    69241 aaaaaagaga aactttcatt ttatgtttaa tcgttatttg aacggattac aaatccgcgt
    69301 tttttatcat tgtgacgttt accgccatct ttttggctgt tattattttt gctataaccg
    69361 ccttttgatt tgtttccacg ataattaccg ccgtcacgac gattacggtt gtttccgcca
    69421 ccacgtttgc catttttatt gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg
    69481 atcttcactg gcactgcatc agctggatct tttgctgtcg tttttagcaa aagtgcaact
    69541 aaatcttgtg cagaatattc ttctaataat ttatctgctg tttgcaggta tttatctaga
    69601 ccattttctg caagttttgt ttcgatttgt tcaactgcag cacctaattg acctttgaat
    69661 gcttcttttt cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc
    69721 acatgcaagt agtccatttc atttggtgta acgaaagtca cagacatacc gccttttccg
    69781 gcacggcctg tacgtccgat acggtgaacg tagctttctg gatcttgagg gatatcatag
    69841 ttgtacacat gagtgacacc tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca
    69901 aggatatcta aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac
    69961 aaatcgccat ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg
    70021 tctacacggc gttttgtacg tccgaagacg attgttaatt caggtgtttg aacatcaaac
    70081 aagcgagtca tgatatcgaa tttttcgtat tcttttgcgc ggacatagta ttgatcgatc
    70141 aagtcagctg tcatttcttt cgctttgatc ttcacatgat gtggattttt catgaattta
    70201 acgccaatat tcttgatagc tggcggcatc gttgctgaga aaagtaatgt ttgtctttga
    70261 tcaggaactt gtgaaatgat tttttcgata tcttccaaga agcccatatt caacatttca
    70321 tctgcttcat ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg
    70381 tggtcaagca tacgtcctgg tgtacccaca acgatatgtg gacgatcttt caagccacgg
    70441 atctgacggc caatatctgc tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc
    70501 cctaaacgat ataattcttc ttgtgtttgg atagctaatt cacgtgttgg tgcgataact
    70561 aaaccttgta attcgtgacg atctggatcg attttttcta acattggtaa tccaaatgca
    70621 gctgttttcc ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc taatgctagt
    70681 ggaatcgttt ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc gactgatttc
    70741 aacaattctg gtgataattc tagttcttta aatttcaaat gaatcctcct atatggttgt
    70801 taactgagga tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt
    70861 ctttttgaca gtggccacgc acgtttttat ccattcttag cccctgtggc accaccgaaa
    70921 aatcactttg cgaagtttag gcattagaaa agccaaacgg gtcgcgttca gctttatcat
    70981 tattatgaca tctggcttca acaagttgtt tctatggcaa aaccagtcca atttctcttc
    71041 atctttcggc aataggttat tctaacatag tttttcgttt tctgcaagtt tacagatttt
    71101 tatttcattt gctgaagttg ttctaccact tcaaataagc ccatgctgtt gctgcctttt
    71161 agcataatac tatctgttgc tgcaagattt tgtttgatag catcaatcaa tttattcttt
    71221 tctttttcaa aatagaacac aggtagttgg gggtattttt ggttcaattc ttcttttagt
    71281 gcatgcattt gttccccata aaggaaaatc atggcgaatt tctcaggatc gatatgttca
    71341 ctcatttgcg catgcatatt taaagactct ttccctaatt ccagcatatc tgctaacaca
    71401 gcgattctgc gtccttttgt tggcagattt ttaaaagtat ctaataccag tcccatggct
    71461 gttggatttg cattgtagac atcgctcaaa atagctgcac catttcctgc cgttagccat
    71521 tcggttctgt tttgagtgat agaaacatgg gcgagccctt ggcgtatctc aggtatggac
    71581 agtccaaaga agcgcccgat cgtatacgca atcaaagcat tcgttacatt ataacttcca
    71641 ggcagcggga tcgtaaatgt ttctccttct actacaaaat cggtttgtgc ttttcctttt
    71701 gctgtcactt ccgcatgaac atcgccattt ccaataccga atgttgtgac tgtttgtact
    71761 aatgttgccg ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag taatccgtct
    71821 gatacaagtc cgtcagtgat ttccattttg gctttagcga ttccttctcg cgacccaaga
    71881 ttttctatgt gtgcttcacc gatcattgtg attgctgcag cttttggttg agccataagt
    71941 gaaagttccg tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat cttttctgta
    72001 tcttggggca tgtgcaaaat cgtgtaagga agaccaatat tattgttgta atttccttgc
    72061 gtcttatatg ttttgtattt ttcagacaag acagacgcgg ttagatcttt cgtggtggtt
    72121 ttaccattgc ttcctgtaat tgcaacgact tctgcgccta ctttttccaa gtaataggtt
    72181 gccaattgct gcatggcttt caaaggatct gctacaggaa taactggaaa attttttgga
    72241 gcttgttcaa caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg
    72301 ataaattcat gtccatctcg cgtaccagca agaggaacga ataaacttcc ttcggttatc
    72361 ttccggctgt caaattcgac attggtaatc tttgtcagtg cagagcattc gctgcccacg
    72421 gcttgtgcaa tttcttgtat tgttagattc attgagatct ctcctaaaaa aggttgtgaa
    72481 aaaagcgttt ggcctaaacg gcgggttcgc atgactctca cttccaagta aatttctcta
    72541 ttcgtattta gaagctagaa tgaacgcggt tacgcaatca ctccagcctc ttctttattt
    72601 ctctttattc atttttttca aaaaaagatt gacgctgatg gtatcgattc attcctaact
    72661 ggatcagttc ttcaatcaaa tcaccgtatt tcaagcccat attttcccat aagagtgggt
    72721 acatactgaa ctccgtaaat cctggcatag agtttaattc attcaggaat aattcatttt
    72781 tatttgtcaa aaagaaatcg caccggctca atccgcttcc acctaacatc gtgtaagcta
    72841 acttcgcgta ctcttgcgct ttttgataaa cttcttctgg cacttcggct ggaatctgca
    72901 tttcgatttt attattgata tattttgctt cataatcata gaatgctacg tcttttacga
    72961 cttcaccagg caaagtcgtc cgaacgtctt catttcctaa tacagcaact tcgatttcgc
    73021 gcgcttcaat tccttgttca acgattgctc gagaatcata ctgataggct gttgctaaag
    73081 cattttgcag ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa cccatattcg
    73141 ccggtttgac aaacatcgga taaagcaaag aaccttcaca ttgatcaaat acttttttag
    73201 gattttcttt ccattgattc ttaagtactg gtacataagg aacttgcggc acaccagcag
    73261 cttgtaaaat atacttggtc atgattttat ccatggcaca tgcactggtc aatacgcctg
    73321 cgccgacata aggcatattc aatgtctcta agaagccttg gatcgttcca tcttccccgt
    73381 ttggcccatg taaaactgga aaaacgatgg ctccttcttc tttgatttcg cccggattga
    73441 tcacttttcc tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa agatgcaaga
    73501 catctttgct ggcaggtttt tctgttagta atggaccttt gacccattgt ccttcttttg
    73561 taataaatac gagttgaact tggtaataat tataataaat ggcatttaaa actgaaaatg
    73621 cggaaagaat ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca
    73681 aaagattgtc ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa
    73741 acaaaaataa acgaataact tatttataaa aaaacgagcc agcttctaaa agaacgagcg
    73801 tcatttattt tttgtcagct agattatttg tcccgttttt tataaaaagt tatgataaag
    73861 aaaaggaggg atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg
    73921 agtgttcatc aattacaatg tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt
    73981 aaaaaaagcc tttgaagaac taggccccac cttcatcaag ataggacaga tgctttctgt
    74041 tcgagaagat ttgctttctt ctgccttcac tcaaacattc aaaacattac aagatagtgt
    74101 acctagcgac acattttcaa cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt
    74161 atcagatata ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta tggggcaggc
    74221 tcatcgtgcc aagctgaaga acggtgacgc ggtagtcgtt aagatccaac accctaacat
    74281 cgccgaagag atccgacttg atcttcaact atttgaacga gcgatccctt tgatcaaata
    74341 cattcctgaa actagcgtag ttgatctaaa aggggtactc caagaagtaa aacgttcctt
    74401 gatcaatgaa atggacttct taaaagaaag tcaaaatggc gaacaattct atcaaaagaa
    74461 caatggctgg aaagagatcc gcagccccaa gatttatgac gctttttgtt cgaaaaaagt
    74521 gatcgtgatg gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa
    74581 aactgaaact tttatcactg gaattcaaaa caagcagctg aaacaagaag ttgcaaagct
    74641 tttagttgag aactttatga aacaggtgtt tgatgatggt tttttccatg ctgatcctca
    74701 tccagggaat ctgctattcc atgtactaac aaaagaagaa cagacacaag ctagccgaaa
    74761 aactgaaaca gtccatgaaa aagagttcgg ttcctttgct tttcgagcct ccacttctgc
    74821 agaagaccct gttgcgccat ataccatcaa ctacatcgat tttggtatga tggggcatct
    74881 ttctgcagga cttaggcaaa aactcacaca agccgtttta gcgctgtata cgaaagatgc
    74941 ttatcgaatc gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag
    75001 tcggttccac caagaactga caagttttct tgaacagtat tatgattctc ctattgatga
    75061 aatcaacttg caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca
    75121 gttcgatcgt gatatcacgc ttttgctgaa agcatttggg acattagaag gggtgatccg
    75181 cgtcctcgat cctgaagttt ccctcatgga agtcgcttcg ccttttgctc aacattattt
    75241 tctcactcat ctggatgtag aagatacatt gaaacaaagc ggattagact tattggaagg
    75301 gatgaaggct gcgcctaaga tccctcaaca gctccaccat ctattagaaa tgtggacttc
    75361 gggacaagga aaagtcaatt tggaattgaa aaagcaagac aaattattga gcaggatcga
    75421 aagtatgatc aatcggcttg ttttcggtat gattttagct gcattgatcg tagggtcctc
    75481 cctgcttgtc caagcagcac ctgtagaaaa cgcagaggtc gtttccttat tagggatatt
    75541 tacgtatgct attgccgcat tcgtcattat ttttttggca atcgatgcat tgatccaaat
    75601 gtataagaag aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct tcaagaaata
    75661 agaaggggtt ttacataaat tgtttctcaa atttttgtga aaattcggct tatttttcga
    75721 agaggctact tctgaaacac cgagaataaa aaatgtgaaa aagctgttct gcacccagta
    75781 ataagaacaa tcaaaaaggg aacatggaac acatttatcc tgtttccctg ttcccttttt
    75841 gatgtattcg agcataaata aaataccttc gttacctact atcttctgta cttggtaaac
    75901 cttagtcaat tgcctttttt aacgcttgac gaaaagacat atgatctttc tccgtaaagg
    75961 gttttggacc ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt
    76021 ctagaagcgt gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgca
    76081 cacccttcgg caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct
    76141 tcttaaccat accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga
    76201 tttggacatg attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca
    76261 cgtcaagacc ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc
    76321 catcaatgaa tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat
    76381 ccgctcagta aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct
    76441 atcggcactg attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct
    76501 tttgctaaaa caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt
    76561 tgagtagtat acttgacaac gatccggcga tccattggaa tagctgtatc agtttgataa
    76621 tattccttat ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct
    76681 atcgcaaaac catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc
    76741 atagccaatg gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca
    76801 atcaagcctg ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacagt
    76861 atgccaacat aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa
    76921 gtaaacagat ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac
    76981 acgaacaaga tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg
    77041 aataacaatc gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca
    77101 agctctcgcg tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga
    77161 taatgactaa ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct
    77221 gcaatctgag tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct
    77281 gcaaataagt actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg
    77341 accgtggtat tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa
    77401 taaaataaag gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc
    77461 agcagagcga tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa
    77521 aatagtccag tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta
    77581 tgcgagtctg ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag
    77641 ataaagggcg ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca
    77701 atggctcctt aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa
    77761 taaaccaatt ttacagaata tattcttgta aaaaaaaata cagaaagaag cataagcttc
    77821 tttctgcatt tttacggtac ttgattactc aagcagacaa gcccttctcc acaaaagtct
    77881 tgcccgacac ccctgcatcc ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc
    77941 aaaatcaatt aggagctctg taaaccagag tccttctcga cccgtcgcat aaatttactt
    78001 taacacagtt cctatgttta tgcgagagct tttttcattt tataatcgac catttgaaat
    78061 ccttgaccga tatctgaaat aagttcttct gtaatctcaa atccttcatg cttataaacc
    78121 tcgattgctt gatgattccc ctgattcact cgcaaaaaga tctctctttc ctttgtttgc
    78181 gcagcaagtt cttcatacca gcgaaaaact gaagaagtca atccttgacc acgtacttta
    78241 tttagcaaat ataatttact taaataaatt tttccattta tctcttcata agcagtgtaa
    78301 ccgacagtct tttcttcgct ttttaataaa aagtaggaca caccttctgt cagttctttt
    78361 tgaatctgtt tttttgactg ataagttttc agcatgtaat ccacttgttt ttgaccaatg
    78421 atcggcacaa aagcttccgg ccatacttct tgtatgactg ccatcagctc atccagttca
    78481 ttttctgtta cttttatttt ttccatacac ccacgtcctt ttttacatta taaatgacct
    78541 tgcgcttgat ggaataaaaa gagcatgatc atgctataca cacccaccga ccatggtttc
    78601 aagaaaatca caatctgcat aaattcccta aaagataatt tcgtcaaacc agctactaga
    78661 cagatcaagt cgtctggcgc aaagggaagc agcaaggtaa caactagtac ttttttaata
    78721 tctttcgttt ttcttgtcag caattcatca aatttctgat acttttttgg cgataaaata
    78781 agctggacaa aggctacgcc atagtaacgg acaagcaaaa atccgattgc ttccccaatc
    78841 actaggccaa tacaactata taaaagccca aatccattgc caaacatcag catgcccgct
    78901 actgaggaga tccctcccgg caaaatagga atgatcactt ggatcacttg caacagaaca
    78961 aatatcatca ctgcatactt gccaaattgc tggataaacg cttgcaaaga cgtgctcgat
    79021 cgaaaaatcc catgatgata agcataaaga atcaacacaa agaaaaaaaa cagaccaata
    79081 atgggcatga tctgaagaca tttttgtaaa actgatcctt ttttagacat ctatattccc
    79141 cacatctttt ttcatttatt aaaactgcct cct
//
LOCUS       DAZKSU010000003        75802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000003 DAZKSU010000000
VERSION     DAZKSU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 75802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.003"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            548..1351
                     /locus_tag="ACG8Z3_000170"
     CDS             548..1351
                     /locus_tag="ACG8Z3_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0822523.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            1936..2391
                     /locus_tag="ACG8Z3_000171"
     CDS             1936..2391
                     /locus_tag="ACG8Z3_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822524.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            2510..3568
                     /locus_tag="ACG8Z3_000172"
     CDS             2510..3568
                     /locus_tag="ACG8Z3_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFQ0822525.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            3610..5559
                     /locus_tag="ACG8Z3_000173"
     CDS             3610..5559
                     /locus_tag="ACG8Z3_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0822526.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            5562..9203
                     /locus_tag="ACG8Z3_000174"
     CDS             5562..9203
                     /locus_tag="ACG8Z3_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3659 domain-containing protein"
                     /protein_id="HFQ0822527.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            9603..9941
                     /locus_tag="ACG8Z3_000175"
     CDS             9603..9941
                     /locus_tag="ACG8Z3_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822528.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            10027..10272
                     /locus_tag="ACG8Z3_000176"
     CDS             10027..10272
                     /locus_tag="ACG8Z3_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822529.1"
                     /translation="MREESKCKMVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQF
                     YDYRNGSKKIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            10292..12448
                     /locus_tag="ACG8Z3_000177"
     CDS             10292..12448
                     /locus_tag="ACG8Z3_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822530.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            12460..12693
                     /locus_tag="ACG8Z3_000178"
     CDS             12460..12693
                     /locus_tag="ACG8Z3_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0822531.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            12683..13549
                     /locus_tag="ACG8Z3_000179"
     CDS             12683..13549
                     /locus_tag="ACG8Z3_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822532.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            13564..15534
                     /locus_tag="ACG8Z3_000180"
     CDS             13564..15534
                     /locus_tag="ACG8Z3_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822533.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            15701..15928
                     /locus_tag="ACG8Z3_000181"
     CDS             15701..15928
                     /locus_tag="ACG8Z3_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822534.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            15925..16419
                     /locus_tag="ACG8Z3_000182"
     CDS             15925..16419
                     /locus_tag="ACG8Z3_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822535.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            16594..16929
                     /locus_tag="ACG8Z3_000183"
     CDS             16594..16929
                     /locus_tag="ACG8Z3_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822536.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(17186..18769)
                     /locus_tag="ACG8Z3_000184"
     CDS             complement(17186..18769)
                     /locus_tag="ACG8Z3_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822537.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            18891..19475
                     /locus_tag="ACG8Z3_000185"
     CDS             18891..19475
                     /locus_tag="ACG8Z3_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822538.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            19486..19731
                     /locus_tag="ACG8Z3_000186"
     CDS             19486..19731
                     /locus_tag="ACG8Z3_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822539.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            19737..20459
                     /locus_tag="ACG8Z3_000187"
     CDS             19737..20459
                     /locus_tag="ACG8Z3_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822540.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            20590..21333
                     /locus_tag="ACG8Z3_000188"
     CDS             20590..21333
                     /locus_tag="ACG8Z3_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822541.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            21339..21704
                     /locus_tag="ACG8Z3_000189"
     CDS             21339..21704
                     /locus_tag="ACG8Z3_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822542.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            21720..22250
                     /locus_tag="ACG8Z3_000190"
     CDS             21720..22250
                     /locus_tag="ACG8Z3_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822543.1"
                     /translation="MSQNISAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            22412..22780
                     /locus_tag="ACG8Z3_000191"
     CDS             22412..22780
                     /locus_tag="ACG8Z3_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822544.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            22971..23231
                     /locus_tag="ACG8Z3_000192"
     CDS             22971..23231
                     /locus_tag="ACG8Z3_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822545.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            23849..24649
                     /locus_tag="ACG8Z3_000193"
     CDS             23849..24649
                     /locus_tag="ACG8Z3_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822546.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            24686..24868
                     /locus_tag="ACG8Z3_000194"
     CDS             24686..24868
                     /locus_tag="ACG8Z3_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822547.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            25058..25345
                     /locus_tag="ACG8Z3_000195"
     CDS             25058..25345
                     /locus_tag="ACG8Z3_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822548.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            25436..25675
                     /locus_tag="ACG8Z3_000196"
     CDS             25436..25675
                     /locus_tag="ACG8Z3_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822549.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            25803..26132
                     /locus_tag="ACG8Z3_000197"
     CDS             25803..26132
                     /locus_tag="ACG8Z3_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822550.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            26185..26598
                     /locus_tag="ACG8Z3_000198"
     CDS             26185..26598
                     /locus_tag="ACG8Z3_000198"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822551.1"
                     /translation="MFSQEEIISKSQPDKNGTIIMKKKIPCYRCSGSGQYKFMSMGKI
                     TYGTCFKCDGDGFLVVTEKSFTPEYRTKLNVLNKKRADRKIRIAREKESQKNFDRYGF
                     EKGYIYVVIEKNSYTIKDELHHQGATYDPLLGWFF"
     gene            26678..27016
                     /locus_tag="ACG8Z3_000199"
     CDS             26678..27016
                     /locus_tag="ACG8Z3_000199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822552.1"
                     /translation="MKLEEYSKRQQEILSAKRREEGEFIGTIAQRLELDLKLTNTVTI
                     DTQWGNVYLHLFEDQNHNSLIWKTNKFLYYDEWDVGEIRKVKATIKAHDYYRDTKQTW
                     IKNVKIQKNE"
     gene            27031..27348
                     /locus_tag="ACG8Z3_000200"
     CDS             27031..27348
                     /locus_tag="ACG8Z3_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822553.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            27351..27494
                     /locus_tag="ACG8Z3_000201"
     CDS             27351..27494
                     /locus_tag="ACG8Z3_000201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822554.1"
                     /translation="MLSDLLILLIYLGGTEIIAYFLLVIGEYGISSAITQRFRLPVSE
                     LKK"
     gene            complement(27698..28174)
                     /locus_tag="ACG8Z3_000202"
     CDS             complement(27698..28174)
                     /locus_tag="ACG8Z3_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822555.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            28258..29631
                     /locus_tag="ACG8Z3_000203"
     CDS             28258..29631
                     /locus_tag="ACG8Z3_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HFQ0822556.1"
                     /translation="MKKSKRKIATLLTLMVQFTGIAMSVITINPQVLHADGIATYDAQ
                     FNCTTEGSGSGTDSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWK
                     QESGIDPRTVEGYYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGS
                     WWTTEAQLDFMFKGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVR
                     GENAKEIWKYMKSKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDD
                     ANSGSGSIGKEGKINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQ
                     GQCTELTWAYMSQLYDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWA
                     VGSAGHTGVVIAVFDDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGK
                     AKFEIEK"
     gene            29656..30183
                     /locus_tag="ACG8Z3_000204"
     CDS             29656..30183
                     /locus_tag="ACG8Z3_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822557.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            30170..30733
                     /locus_tag="ACG8Z3_000205"
     CDS             30170..30733
                     /locus_tag="ACG8Z3_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822558.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            31151..31888
                     /locus_tag="ACG8Z3_000206"
     CDS             31151..31888
                     /locus_tag="ACG8Z3_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822559.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(31905..32321)
                     /locus_tag="ACG8Z3_000207"
     CDS             complement(31905..32321)
                     /locus_tag="ACG8Z3_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822560.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            32714..33961
                     /locus_tag="ACG8Z3_000208"
     CDS             32714..33961
                     /locus_tag="ACG8Z3_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0822561.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSNPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            34231..36621
                     /locus_tag="ACG8Z3_000209"
     CDS             34231..36621
                     /locus_tag="ACG8Z3_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0822562.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            36670..38505
                     /locus_tag="ACG8Z3_000210"
     CDS             36670..38505
                     /locus_tag="ACG8Z3_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0822563.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            38580..39437
                     /locus_tag="ACG8Z3_000211"
     CDS             38580..39437
                     /locus_tag="ACG8Z3_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0822564.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNVNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            39427..40506
                     /locus_tag="ACG8Z3_000212"
     CDS             39427..40506
                     /locus_tag="ACG8Z3_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0822565.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            40845..41645
                     /locus_tag="ACG8Z3_000213"
     CDS             40845..41645
                     /locus_tag="ACG8Z3_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822566.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            41751..42539
                     /locus_tag="ACG8Z3_000214"
     CDS             41751..42539
                     /locus_tag="ACG8Z3_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822567.1"
                     /translation="MSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWISAVGEEN
                     IEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVSWEMNNQ
                     YKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYAEYLKTE
                     RDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALSQYSSDE
                     EVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            42771..43640
                     /locus_tag="ACG8Z3_000215"
     CDS             42771..43640
                     /locus_tag="ACG8Z3_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0822568.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            43660..44007
                     /locus_tag="ACG8Z3_000216"
     CDS             43660..44007
                     /locus_tag="ACG8Z3_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822569.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            44020..45282
                     /locus_tag="ACG8Z3_000217"
     CDS             44020..45282
                     /locus_tag="ACG8Z3_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822570.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            45362..46738
                     /locus_tag="ACG8Z3_000218"
     CDS             45362..46738
                     /locus_tag="ACG8Z3_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0822571.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            46735..47364
                     /locus_tag="ACG8Z3_000219"
     CDS             46735..47364
                     /locus_tag="ACG8Z3_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822572.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            47376..47846
                     /locus_tag="ACG8Z3_000220"
     CDS             47376..47846
                     /locus_tag="ACG8Z3_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822573.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            47843..48325
                     /locus_tag="ACG8Z3_000221"
     CDS             47843..48325
                     /locus_tag="ACG8Z3_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822574.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            48322..48984
                     /locus_tag="ACG8Z3_000222"
     CDS             48322..48984
                     /locus_tag="ACG8Z3_000222"
                     /inference="COORDINATES: protein motif:HMM:NF019467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0822575.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            48971..49339
                     /locus_tag="ACG8Z3_000223"
     CDS             48971..49339
                     /locus_tag="ACG8Z3_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0822576.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            49420..49866
                     /locus_tag="ACG8Z3_000224"
     CDS             49420..49866
                     /locus_tag="ACG8Z3_000224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822577.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRITEQAIEKQKDKEKKEIMSIYLSSGASFFHEID
                     SMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            50575..51174
                     /locus_tag="ACG8Z3_000225"
     CDS             50575..51174
                     /locus_tag="ACG8Z3_000225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0822578.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            51190..51666
                     /locus_tag="ACG8Z3_000226"
     CDS             51190..51666
                     /locus_tag="ACG8Z3_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822579.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            51683..52585
                     /locus_tag="ACG8Z3_000227"
     CDS             51683..52585
                     /locus_tag="ACG8Z3_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822580.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            52658..52849
                     /locus_tag="ACG8Z3_000228"
     CDS             52658..52849
                     /locus_tag="ACG8Z3_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822581.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            53013..53600
                     /locus_tag="ACG8Z3_000229"
     CDS             53013..53600
                     /locus_tag="ACG8Z3_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822582.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            53600..54499
                     /locus_tag="ACG8Z3_000230"
     CDS             53600..54499
                     /locus_tag="ACG8Z3_000230"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFQ0822583.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            54499..55413
                     /locus_tag="ACG8Z3_000231"
     CDS             54499..55413
                     /locus_tag="ACG8Z3_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822584.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            55465..55680
                     /locus_tag="ACG8Z3_000232"
     CDS             55465..55680
                     /locus_tag="ACG8Z3_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822585.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            55865..56119
                     /locus_tag="ACG8Z3_000233"
     CDS             55865..56119
                     /locus_tag="ACG8Z3_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822586.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            56298..57341
                     /locus_tag="ACG8Z3_000234"
     CDS             56298..57341
                     /locus_tag="ACG8Z3_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0822587.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            57514..57738
                     /locus_tag="ACG8Z3_000235"
     CDS             57514..57738
                     /locus_tag="ACG8Z3_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822588.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            57741..57998
                     /locus_tag="ACG8Z3_000236"
     CDS             57741..57998
                     /locus_tag="ACG8Z3_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822589.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            58100..58453
                     /locus_tag="ACG8Z3_000237"
     CDS             58100..58453
                     /locus_tag="ACG8Z3_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822590.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            58506..59300
                     /locus_tag="ACG8Z3_000238"
     CDS             58506..59300
                     /locus_tag="ACG8Z3_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822591.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            59599..60447
                     /locus_tag="ACG8Z3_000239"
     CDS             59599..60447
                     /locus_tag="ACG8Z3_000239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822592.1"
                     /translation="MIEYDFNEAYSRIMRNYPLPSNTYLKAKYNTDQAILRAINRPKV
                     RPYENLRTYMFFLVNFEGTAKEGTYANPKSQSMFADYGENPKTANEPIWLSEIELTLI
                     LDYYDIKEMEVLDSHTFLTKKGLLKEYFERIEPLKTDPDTLKLYKNLRTKVFGAIGQK
                     SLREGETSTFDKYSMYNRAYSSMVAGVFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRS
                     EVPEFEELYKLGVVKKKVHTISKEEVEKALEFKNKGFENKGSFGKSKDEVKGVVIDDD
                     FEFDFD"
     gene            60480..60800
                     /locus_tag="ACG8Z3_000240"
     CDS             60480..60800
                     /locus_tag="ACG8Z3_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822593.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            61055..61744
                     /locus_tag="ACG8Z3_000241"
     CDS             61055..61744
                     /locus_tag="ACG8Z3_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822594.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            61781..61948
                     /locus_tag="ACG8Z3_000242"
     CDS             61781..61948
                     /locus_tag="ACG8Z3_000242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822595.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            61968..63596
                     /locus_tag="ACG8Z3_000243"
     CDS             61968..63596
                     /locus_tag="ACG8Z3_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822596.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            63647..64018
                     /locus_tag="ACG8Z3_000244"
     CDS             63647..64018
                     /locus_tag="ACG8Z3_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822597.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            64103..64303
                     /locus_tag="ACG8Z3_000245"
     CDS             64103..64303
                     /locus_tag="ACG8Z3_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822598.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            64437..65663
                     /locus_tag="ACG8Z3_000246"
     CDS             64437..65663
                     /locus_tag="ACG8Z3_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822599.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            66260..66655
                     /locus_tag="ACG8Z3_000247"
                     /pseudo
     CDS             66260..66655
                     /locus_tag="ACG8Z3_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            66733..67772
                     /locus_tag="ACG8Z3_000248"
     CDS             join(66733..67230,67233..67772)
                     /locus_tag="ACG8Z3_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611651.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFQ0822600.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIGT
                     LDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLEE
                     NARRLALIFLPPYSPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNERP
                     LEVIDRLCVRM"
     gene            67785..68723
                     /locus_tag="ACG8Z3_000249"
                     /pseudo
     CDS             67785..68723
                     /locus_tag="ACG8Z3_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            68716..69063
                     /locus_tag="ACG8Z3_000250"
     CDS             68716..69063
                     /locus_tag="ACG8Z3_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822601.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            69060..69242
                     /locus_tag="ACG8Z3_000251"
     CDS             69060..69242
                     /locus_tag="ACG8Z3_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822602.1"
                     /translation="MNIIKKYEFGYITYEQLIEELWGYGQQLINEVGIDCFCFYIESG
                     AGYHKNRYYIFPYPPK"
     gene            69378..70235
                     /locus_tag="ACG8Z3_000252"
     CDS             69378..70235
                     /locus_tag="ACG8Z3_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0822603.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            70255..70815
                     /locus_tag="ACG8Z3_000253"
     CDS             70255..70815
                     /locus_tag="ACG8Z3_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HFQ0822604.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            71550..72317
                     /locus_tag="ACG8Z3_000254"
     CDS             71550..72317
                     /locus_tag="ACG8Z3_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFQ0822605.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            72343..72888
                     /locus_tag="ACG8Z3_000255"
     CDS             72343..72888
                     /locus_tag="ACG8Z3_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822606.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            72889..73455
                     /locus_tag="ACG8Z3_000256"
     CDS             72889..73455
                     /locus_tag="ACG8Z3_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822607.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            73470..73718
                     /locus_tag="ACG8Z3_000257"
     CDS             73470..73718
                     /locus_tag="ACG8Z3_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822608.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            73737..73976
                     /locus_tag="ACG8Z3_000258"
     CDS             73737..73976
                     /locus_tag="ACG8Z3_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822609.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            73993..74577
                     /locus_tag="ACG8Z3_000259"
     CDS             73993..74577
                     /locus_tag="ACG8Z3_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822610.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            74684..74965
                     /locus_tag="ACG8Z3_000260"
     CDS             74684..74965
                     /locus_tag="ACG8Z3_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0822611.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            74955..75296
                     /locus_tag="ACG8Z3_000261"
     CDS             74955..75296
                     /locus_tag="ACG8Z3_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0822612.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT        28549 a        10155 c        15158 g        21940 t
ORIGIN      
        1 ataaatataa ataaaagggg aagctgaagc ttcctccttt tccttttttt caattattca
       61 aagttacaca agcaaaataa actcctagat agtgttagtt ttttttaaga ttttttattt
      121 ttaagatttt gaaataagag aggagtaatc ctcctttatt aaatatctgt taatatatct
      181 gttaaatatc tgttaatata tctgttaggg gaggcttaat ctcttgggag agtaagtatt
      241 aaaggggtat aaaggtaaca cttttggaca gtaaaggtaa caaaagtgga cagtaagggt
      301 aacagttttg gacagcaaag gtaacaaaag tggacatttg aggtatcaca ctaggataag
      361 acgttacctt cggttttctt gatttggaag gcgaaaaaat agaaaattag attagtaaca
      421 aatctttgtt gactgttacc tttagttgtg atttaattta ggtaacaagc agaaaaaatt
      481 gtttccttta attagtacca gattaacatg attaaaggag cgcaaaaaga agattggaga
      541 gtaatcaatg tctcaagaag ttaccgaaaa gattattgat agtgatgtag atttagtatc
      601 agaacaaaca aatgcaattg tgcgttataa caatggtctg aatacaacag ctttgcgagg
      661 atttaagcct gttgaaatgg atgtgttttg ggctatttta tcaaaaatga aacgtcaagg
      721 aacagcagaa gtaacttttg atttcaacct ctttcgttat ttggtcaaat acgacagaag
      781 agaaaaggac agtttttatt ctgtgttaaa agatttgagt gacaaactag gtaatttaac
      841 ctaccgtttt gaagattcag acgtctatga acagctgtgg ctttttcaaa aattccggat
      901 agataaacag aaggaaacag ttacaattaa ggcaaatgag gaatttgaat ttatcttaaa
      961 ctcaattggc aaaaacttta ctcgttttga acttgataat tttacaaaac taagaagtag
     1021 ctacacaaaa gaattgtatc gtcatctcat gcaatacaga aataaagagt ggcgaaatgg
     1081 ttattgggta gtcaaagttg aagattttag aagagcatta agtatacctt caagttatag
     1141 aatgacaaat attgataaaa gaatactaga gcaggcgaaa gaagaatttt tagctcctga
     1201 tgaaaacgga atacaggtat ttgaaacttt tgattatgaa aaaatctatg caaagaaagg
     1261 aagaagagtt gatcggctca gattcacttt tagtgagcca gaatcaaact tgccaaccat
     1321 ttcaatgcat aattggttag aagaagattg agaagaagca ggttgtaatt ttaatacaac
     1381 ctgcttcttt aaaaaagtat gggtaacact tttggacaat aaatgcaaga agtttttttg
     1441 taagcaagtt tcagtctaaa taatatttga aagaatggct aatctataca aattttaaca
     1501 actatgggta acacttttgg acaagtcaag aaaaaaaaca atttttctta atttgttgat
     1561 cgactaaagg taacactttt ggacaacaca gaataaaaag tccggtgata tcagttttgg
     1621 taggaaacat cttaatatct ttgtgagatt gaaggtaaca tttttggaca agtcaactat
     1681 ttgttaaaag ttaatataga gtaattagat aagttttttc ttttcgtctg ataaatggat
     1741 aatattagtt attttattgc taattagtct ttcatgatga ctgaaatagc taaaataaat
     1801 atctcgttgg ataattgctg atactttaag ggatatctaa atttgctgat tgatttattt
     1861 atgttattat gatattataa taacataaat gtgttacgcc cgaacctcat tttaaaaaaa
     1921 tgaaggggtg agacaatgaa tgctattaat tggcatgaat ttgttaacta tatggagagc
     1981 ttactagatg atctaaaaag ttttagtatt gctgatgcag taaagcatgg gttaccagtt
     2041 agtacaacga tgaaattacg aggattgaaa attccagatg aaattattaa gctgagaact
     2101 aaaacaatta ttgaaaccgc aaaagtcgtt gacaatatga accatgaact aaaaggcaag
     2161 aaaacaactt acgagaaaga ttttgaacgt tttttaaaat ctgttattgg aaaatatcta
     2221 ggtaaaaaat ctttcagtga attgagagat gaagagattg tacctactcc aatggttagc
     2281 aaaatccgaa aaaacaaagc cgaggggaat tactatcttg gtacaatagc aaaaatcatt
     2341 gattatttgg aaaataaaga gcaaaagaaa ggaacgaaga aaggaagata atttttgtgt
     2401 taaatattaa tggggaagaa tataccgaaa tgattcaaaa tcccgaacct agaattaccg
     2461 aaaaaacgtc tgattgtttg ctcttttatg aaagggggca gacaaactaa tggaaaaaaa
     2521 cgtcaattca acatctacca aagatcaata cagtattatt ggagggttgc ttggtgataa
     2581 ggataagaga acacgtcacg gattacgcat tatgcttgca acttttctag gagtgctttt
     2641 tttattaaca ggcttgggta gctacattta tcaattagga aactataatt attatctcgt
     2701 gaacaataca acacctatag gctcttcatt ggagtttgct cgatcaggag cagagatcac
     2761 tatttcagat gtgtggacag atcaaaacag agatgtaaca gtagtaaaac tcggttactc
     2821 gcaaaacgct agaaagttgt tatctacgca aggaaaaaac tacaacttat atatggcaac
     2881 gaaagaaaaa gatcgtccta aaaagcttga aatgagctat gggatgttaa gtactgatgg
     2941 ggatgctttc ttattcctaa aaggtaaatt agatgaaaga gcttatcaag tttttattgc
     3001 aaaccaagta tcactaattt ctggtgatga tacagctgac gatgatacca aagtcaatgt
     3061 gaatgaagca gattcagtta caaaagcact ttctcaatat tctatgaatg atgttaataa
     3121 tgaaggagta atttcttttg ggaaaaaagc aaaacctgtt aatactcctg ataatatcaa
     3181 tttcagaata aatccatatt cagattcaac taatgtctat caaggaagct ttcttaccaa
     3241 agatggcgat attgattatt caaaagttgt agcagttaca agtaagaata atgttgtaag
     3301 taagttagaa gatcagttaa atgcttcaaa tactaacatc aagaaaattg aagctacaaa
     3361 agctgagtat ctggaacgtt tacaacaaaa tcctgacgat aaagattcgc aagaaagtat
     3421 tcgtcaacta gacagtcgaa tggagaccga aattaaagca atcaatgata cacaaaaatt
     3481 gattgatcaa tacaaaaatg ctgactttaa caaagattct tttggagaca tgaagacgac
     3541 atttaagtac ctaactctga ctgactagaa gtaaattatt cttattttag aggaagtgaa
     3601 cacaaacaga tggaaaatag taccgagttt tttaagaata ttcaaactga atttaatcat
     3661 gctctcacac caatgaaacc accgaaacat aagttagtgg gacgtgatga cgaactccat
     3721 gagttgtcac tgatcttgaa tagaccggaa acaccgcaag ctatcttgtt aggtgaagcc
     3781 ggaacaggta aaacagcact tgcaagtgaa tgggctgaaa gacagcgaca agatggaaag
     3841 aaagtacaag tatacaaatt atcaatcgga caattgcgtg ttgggggaga tgatgtcctc
     3901 caaacacgtt tagaaacatt aattccaaga ttgaaagaat atgagcgaat tttaaaatct
     3961 gaggacaaag aaaatgaatt tgtcctcttc attgacgagt ttcataaagt tgttagctct
     4021 tttggtagtg gcaataaaat tgggggtgat tcgctaaaag aaccgttagc aaatgaatca
     4081 cttcaattga ttaccgctac tacaagaaaa gaatatgaaa actatatcaa aagagatgat
     4141 gccttaggga gacggttaca accgttagaa ttgaatgaac taaaaagcga tgttgtcgtc
     4201 gatattttga gacaatggct aaatacatcc tctgttaaag atgatcctca ggcttctttt
     4261 aaattatcgc aaaggattcc tgatgaatta cttcaagaaa tcgtacaagc aaacaagctt
     4321 tatcgtgagg atttgtccga accggctaaa tcaattgccg taatggagga tttgattgca
     4381 aaagatcgag tagacggaaa tgcttataat cgagaaagtc tattgcaaat atttggtaag
     4441 tataatattc agctagattc tgattttgat atggaaaaac ttcaagagga attaaagtcc
     4501 ataaaagggc aaccgttagc agtttatact ataacaatgt tgtttaaacg tatgaagctt
     4561 ggatttgatc gttttaaagg tcgaaagcaa ccaaaagcaa ctgctctgtt tacaggatca
     4621 acaggggtcg gtaaaactgc aatggctaaa gccgtggcaa gaggacttta tggcgatgaa
     4681 aataaagttc tcttagttaa tatgccggac tacaaaacaa aagatgatgc aatgcgtttt
     4741 agacgactca taggtactca tgtaaggcat gatccttctg cgattattgt gtgtgatgaa
     4801 tatgagaaag ctggtgtaga aacactcgat gcctttttat caattttaga tgaaggtagg
     4861 ataagtttta ctgaaagagg atgggatgat cgagaagttc acgacactgt ttcattgtca
     4921 aatacaattt tatttgcgac aacaaatgca ggtgcggaga tgttcgattc gttaaacaga
     4981 tatagctcac tatctcgtgt tacaaacaag gaaaattcta atgaatatag cactgaacta
     5041 aaaagagaat accgaaaagt cgcatcaggg atcaaacaag ccttgattgg tgaaggtcta
     5101 aagcctgaag ttattaaccg atttggacga atagttccat tttgggcttt atccgaacaa
     5161 actcttctgg aaattgctta taatgaaatg ttgaagagtg ctgaattagt acgaaaacat
     5221 ttaaatatag atttacgtct tccacctagg aaagaaagag tatatggtca aaatttttat
     5281 gcagacgatc tctccatgca cttagttttt gacatggctt cacaagagga ttctaaatca
     5341 gggggtgcaa ggaatatctt gcatttagtt gaatctgagt ttgaagaagc gattactgaa
     5401 gcaataatta cttatccaaa cgcaaaaata ttcgatgtta agttgagtga agccggagcg
     5461 tttgaaggaa aaggtgattc taatgctgaa attattgcaa agccttccgt tgctgaaagt
     5521 aaagaaaaca agattgtatt ttcgattgga ggggcgtagc tatgaaattc aagaaatatt
     5581 tactacaaaa ccttagaata aaacgatggg tcgtaagagg tctagttatt ggaggagtat
     5641 tagcagttac tttaggcgct ccttttggaa tgaatgatcc aactcctgac aatcaagata
     5701 gcaaacaaga aacagttata aataagaacg ttgcatatgc ggaaagtaaa ccggcagaac
     5761 aaggaatgtg gacactatat gctcgtatcc taatgggaga taaagaagtt aatgatagtg
     5821 ccgctaaaac tggacaaatg aatttgttag gacaaggtgg tatcagtgcc gatttccctt
     5881 attctgacat ttctaaacaa ggtaattcga ttgcaggggg taatgcaggt actcaactag
     5941 catctacttt agcaacttat agttactacg gatatattga aacagtctca ggaaatgcta
     6001 ttgccagtca agcatcatcc attctaggaa gtattggtag gacgattggt agttttatcg
     6061 gatcaattag tttaatcctt tctaatattg ctgaatccat aaatgaactc cttggaaaat
     6121 ttttcatgag tatgaatata ttttctctta tggggtgggg agaaggtgga tcggatgtat
     6181 ctaatccggt atctaaagca ttaactagct tgcttaacaa tttgggatta aataatgaat
     6241 tatttaaagc gatagcaagc ttatctatgc tctttttcac agcaatacta ggttatcaag
     6301 gaatgaaatt attgtcgaaa gatggtgtta actcgaaggg attatcacca gtacttgaaa
     6361 aatggattgt tcgtattttt gtattgttta tattcttacc agtggcagga tcgctattgg
     6421 gagtttatat caacggcatc cagtcagcaa aaaaagatgg agatatacca acaaatgttg
     6481 tttctgacta catcattaac acaagacttt gggcttctac tcaaaatctt gctccatcat
     6541 ctgatactgg tggtgatgta cctgacggaa agcctacatc cgaaatcaag ttcgttgata
     6601 cagattatga accatctaaa aaatctgcaa tgataagagc gatcaatact agcagttatg
     6661 ccagacaaaa taatgatcta gattctaatt cagtcggtat ggctctatta aaagaatggt
     6721 catcgaatga tactttcaat attaatactt atgccggtga tgttaatgtc gccgaaaatg
     6781 atgcttatat gggaagagcg cctgctgttt ggacaggcta taatttaggg gatggaaaag
     6841 aaggaagtgc aattaagaaa ccaaccaatt taaatggata tatttggtca gcaaatcaaa
     6901 atgtgacaga cgacaatcgc aagcctgatg gaaaaggaac agcgcataac tttgatgcta
     6961 ctcaaccttg gggcgttgct aacaatgcaa cttttagcac acagtcggta gctttattgc
     7021 tccaatcggc acttgataca tcgggagcta agttttatgc gtataatatc gctccttcag
     7081 gaattcaagg cagtgctaag aatatgtcaa cagtaaaaac agaatggcgt acagcatcct
     7141 tagttggtca tggagctatg ggtaagtttg gctcgtggct agcgatgaac gctgaatcaa
     7201 tcgttttagc agttctttac ttagcatgtg cttgggcgat gttcaatgtg aatatcgtga
     7261 aagcatttaa acgtgaattt attcggattt gggaagtagt tctaacagga tcacttgcat
     7321 caggagcagg tgtgatggca atttcattag gtgttgttac aacatctctg atcggactac
     7381 aattacctat ctttctgatt acttttattc gtgcatttgg tgatactgct gtgggattca
     7441 ttcctaatac attagttggc gatggcgtag caaatattgt tcgatctgct atgattttag
     7501 gtgtaggata tcttttagtc tttaaaaaag gcgaaaaagt aaataatttg attaatatgc
     7561 ttattggctt accacttgaa gtagcgtttg ggtttgatga acgtgctaag ttattatttg
     7621 gtaatagaaa tgatgctgtg cgagttgtgg acggcgtttc taaagcaatc aagaaaagcc
     7681 aaggagctaa aaatccctat tatgatggtg gcaacagctc agtaaaagat atggttgcag
     7741 gaggtcttgg agcagttgga gcgcaaaaaa tggctgaacg tttgaacaat atgggagata
     7801 gcaatgctga tgctaagtcc attgctgata aagtaattcc tgaaacacca gaaggaatgt
     7861 cattcaacgg tggcggagtt ggttccacaa tcaataatac ctctttcaaa gatttagttc
     7921 gtcaagatca actacgtaat aactcccaac aatatattga tcatttagga cgtatggttg
     7981 atgaaaacgg aaactttgtc gatgaaaatg gtgaaatatt aaaagatagt aaatacggta
     8041 aagatgctaa aggcaacttt attgacgaaa aagggcgttt ggtcgatgaa gatggaaacc
     8101 ttatagatac taatggtaaa ccattggaag atagtgaaga tggtaatatt ggtatggata
     8161 gcgaaggacg tttaattgat gaaaaaggtc gcttagtcga tgaagatgga aatcttgttg
     8221 acgaaaatgg acgcaggttg ctaaatggtg attttggtca tgatagtaac ggacgtttag
     8281 ttgacgaaaa aggtcgttta gttgatgaag atggaaatct tgttgatgag tttggtaata
     8341 aacttggtaa acacagcgat ggatataatg tgaatggaga tttagtcgat gaggatggga
     8401 atatcattga gacgagagat gcgcttgtgg cagatcgaaa tagagaactc gaacaacaac
     8461 ggcatacgcc aattgctgtt gcaattcctg agaatgaaga agacgagatt actttagatc
     8521 aaactcatca agaactgagg aatgaaatga gtggtgagga cgtgtcagac agagtgcatc
     8581 gggaattagg agatggagca tctggtgaaa gtcagtcaag agagcttaac agcaactttg
     8641 ataatagtgc tattcggaat gacacagtac ttaaagacag agaatcgcta acaaaagaaa
     8701 cggataaaga acaatcagat gtgaagaaaa agttcattcc tactcctaat gttaatcaaa
     8761 cagataaata tgaaacaaat attggatcag gaatgtcgaa agacaatcaa tcaattagag
     8821 aacctgaact gacaaataaa aataatagaa caaataattc taaatctact tcagataatt
     8881 cagggaaatc taatcaatca accaagaaaa catcaacagc taaatcagcc gttgaatatc
     8941 tatcaaaaac tggtaaaaat gtttacacct cagcagataa agctttagca aaagctccaa
     9001 aaggtagtct agcagggata acctatgaag ctaaaaacga agctattaaa ataggtaaag
     9061 gaacggctaa ggttgccaag gctactgcta caggagcatc caaaactggt aaagtagtga
     9121 agaaatctac catgtcagtc aaggactata ttgtaaaata cggtaatcaa aaatcaactg
     9181 aaaataagtc tgaagacgag taaaaagaag gagcattgat ttgctccttc tttttacttt
     9241 gatttctaac tgaagatgtc aaggaaaatt tctatttatg caaaaaaatt attttaaatt
     9301 gtctataaat gaattaaata tttactgttc aaagttgtat aattaaaaag ggcttatgct
     9361 ggtaaagaac agaattatta tctaaacaaa aacttgataa accagcattt ttagccaata
     9421 aaaaaagtga aataatttaa tggaaaatct cttttaatca tcgaaaattt atgttattat
     9481 gataacataa agatgtaaat atcgagacgt ctttttctta gttagatcat tgacaagtga
     9541 acaaataaga tttgtcgatg atataattaa cttattaaaa atattaaaaa ggaggttttt
     9601 atatgttcga ttctattaaa aatttggtta gcggaatgac gcctgatatg aaaactttag
     9661 tacaagttgt tttgtatttc ttggcattcg ttttttcgtt gaaaccagga ttcactgcaa
     9721 tgaaattttt tggtgatgca aaatggggag atggattaaa atatgttggg gcgattattg
     9781 caatctttat tattcctgta attacaaccg ttatgcttta cacaatgggt actaaaacag
     9841 gtaatgattt gaaccaaaaa gctaatatca tatcagcact tattccagtt gttggtatct
     9901 atctagtttc aaaatttcca aaaaatgaac tgttaggtta aaagtgaatt gaacaaattt
     9961 ggataatatg ccgatttgtt caatttactt tttaccaaaa aactacgctc caagcgtagt
    10021 ttagaagtga gggaagaaag taaatgtaag atggtttttg atagagaaaa aatgttagct
    10081 cacgccaacg aagtattaat gagcagtctg aaaggtaccg aactggcaaa gataatgaat
    10141 atgaatgtta accagttcta tgattatcga aatgggagta aaaagattga aaaagccaga
    10201 cttgaaacgc ttattaaatt cgaaaaagca tatgtatata tgctagataa acagaaaaga
    10261 actattgact gaaaaaagga gttttacaat aatggcaaaa ttcaaagata ttaccgaatc
    10321 tattaattat gtggcagaaa cagttcctgt agttgcactc tctttgaagc cacaattact
    10381 cgaagtgtac aaaaaagaag aaaaagaagc gaaggcaaaa aaagctaaag caaaggtatg
    10441 gaaagaattc gatgcgttgc tgaccaaaga tttaccaaag aaaaattata tggtaattga
    10501 agatgtacaa aagctcaaca gtgagggagt tatagatgag gatgatgcta ttctcgattc
    10561 tgagaagtat gtcgttctat tgaagtcaag tttgaattat gtagctgatc taccagaaaa
    10621 atttaaagag aagctattag caggtgtccg ttttatggat agtagtggtc aattattagt
    10681 tattccggat gatcctaatg tgtttgaatc agaagttgaa gaagctacag acttctatag
    10741 cgcagtagag caattagaag tagctactaa tggattagaa cgaattgctg agaccttcaa
    10801 ctcttctgaa gaagttgaat ttgaagccgt agcagaaaat gtaccagatg aaaaagaagc
    10861 tctttttgtg gatgataaca aaccaaccga agcagaaaac caagaagtaa gcatagaaga
    10921 aacacaaata caaacagaaa cctaccaaca accaaaaatt gatgaaaaac ccgaatccca
    10981 aagtgacaaa gaaaattatg tttatgaagc tgaaaaaatc acatctgaaa gtgatgtacc
    11041 gaaaaactta gatgctgatt ttttagaaaa aaattttgaa cagatcattg ctgacaaatt
    11101 tcaacctgta gttttagatg agattaatga ccctgtcgat ttaaacgagg agatgctatc
    11161 catacctgag ttgaaaccat tgctgttttt agctaaagag agtatgaacg ctcgcatctc
    11221 tattgctaat aataaaatca acagtgaatt aaataagacg aaggagcaat tgatcctcaa
    11281 aggaaagcaa atgttagctg atgagctttt gcggatagag gaagcaacct cattaaatgg
    11341 tcaaaatcag tttacaaata cactatcggt tgctaaagag agctacgaag cagaagcatc
    11401 taaaattgat gatttagtga tggaaaaatc caatatcatt cgtaaagatt atgatatggc
    11461 gaaacaggct tatttggatg ctgaactaaa gaaactagag caacggtatg atgctgagca
    11521 tttaccagaa gttaaacaac aagaagatga atatagaaaa gcaactgaag aaagtcttga
    11581 tcaacagtac caagaagcaa ttgaatctat taaaaacgat gcattgaaac aaagccaaga
    11641 agcaaaagaa gttgtggtgg aacgaattat caattcattg aaaccagaaa ttgaaaaatc
    11701 attaacagac gtgcaagaaa aaactttaga gattgtcgaa aaaacaaagt cagaaaatga
    11761 agctgagttc catgatacaa aagagaaaac aacagatgtg gttgtcaaat attttgcgaa
    11821 caaagaagaa gttgaaaagc aggtagctaa caacacgaaa gaattagaaa caaaacttgt
    11881 agagcaacaa aaaactatcg aagaatataa agatcgaatg aaagaggtca aagatttagc
    11941 agagggttat aaattaaata gcgaaaagtt ggcagaacaa agtgaagctt taagagaaga
    12001 cttatctcgt tcccgaagtg atctagctca aatggcagaa aaaacgaaat cattagcagt
    12061 tgctacagaa aatcaatcaa gttcaaaacg tgattttaca agtaaaaaga tggctactat
    12121 catttctgtc gctgcaatgg cttgcggaac gatcttggga tcgtcctact ttattagtca
    12181 taataatcaa caagcacagg ctcaaactca aacatctatt tcgagagtgg aaaattcgat
    12241 tgggacaaat acgaactctg cttcaacaaa tagtagctat gaagcaacac caactacaga
    12301 gttcaaagtt gggaaagatg ttgtcatcag tattgatggg aaaacaacta aggctgaagt
    12361 gatttctatt gatggagaaa aagcagttgt gaaaactcca gacggaaatc agtacaaaat
    12421 tcaagctcac ttatctgata ataactaagg agggctatta tgcgagaaga aaaaatagtt
    12481 tatccagtaa ttcaaaacaa tcaagaaaac atgacctctt ctcagcactt acaggaagca
    12541 cagctcgtgt atcaggtaaa tgaactacca caaacgaacg agacaatgag caagtcaata
    12601 tctttaattg gcttgcttat tgttgcaata gtgataactg taggtgttta ccgtaagttg
    12661 aacgtgaaag gaagtatttc tgatggcaga taactcaaac tatggattta aacataactt
    12721 acatcaaaaa ccttctgaaa caattgtcag agttgaatgg cttgaaaagc atgtcaatag
    12781 cacctattct gttacagcaa aaagattaat tgtatcagct atagttgttg cagtcgttgt
    12841 tcttggatca attgtttact tagtttctga tacgattaaa ctgaaattta tttttgttcc
    12901 aacgttatta atgataattg gatttcttat tctaattagc cagtatatgt ttattgatcc
    12961 atctagtggt tcattgattt ctggttctat tagactgttc aaatcagtag ttaattcagc
    13021 aagagtatct atggggaaaa agaaaccatt tcgaggcaca gagattttaa aagtgaaaca
    13081 agatggaaca atcgtatatc gagatgggag tgtagcatta atgttcaatc tagatggggc
    13141 tacatcacta accgcttttc cagaagaagt tatggctcag gaatcgaaat caatacaata
    13201 tcaaaatacc agagatcgtt ccttagctga aatcaaaatc acttcatctc aacctcagaa
    13261 tacggaaaga caaactcgaa atatgaaaat acaaaaacga aatgcaagga gtaacaaagc
    13321 agtctttgac ataatagatc agcaagaacg atttgtatca gaatatattg atggtgtgaa
    13381 aacaacagta atccaatatc ttatcttgaa atctcaatct aagatggtat tggatgaagg
    13441 tgtggaacgt ttagaaaact atacgagtag aggactttat tatagtgtca ctcagttgtc
    13501 agctaaacaa acaaaagaaa tattatctga tatttatgca ctgaaataga ggtgagagat
    13561 taaatggaaa ttatgggtag atcacaacta ttaaatttca aaaaaaagag aaaaactaaa
    13621 aaagaacaaa aaactagaaa ttcaaaggta gtgaataata cctttgttag tgatttaaca
    13681 tctttagttg gttatcaatt tacaccatct tacattagga caggtaagcg gtggggaaca
    13741 attgtgaaat tagtgaatac ttatggaatg aatagaaatt atggatttgg ttggatggtt
    13801 aaagctattc ctgaagtgag agtagaaggg gtaaaagcct atctaattga agcagataag
    13861 ccagtgtcac aaaaagatca acaagaaata atgcacgaga atgttggggg atcaacgaat
    13921 agttatgaaa ataatttaga aggaaaaaat tcagatgctg aaattagcaa aactgttatg
    13981 cggattatgg atttgaatac agcatcttta ctagacagca tggcaaataa aattattgat
    14041 tggaagctag aaatcctatt agtatccgat tctcctaata aaatcagcga acagctaatt
    14101 aaactccaac ggttgtatga tgaaaaaatg tatggattaa agcttatgtc atatggagga
    14161 gatcaacgtg atctgtttga tagactttta agtgaaccaa tcgggaatgt ctatgagtat
    14221 acgtctatga gttcggattt tgcaggaaat gatcatgcaa ttcgcaaagg tttagatgat
    14281 gcgaatggat gggctatcgg cactatgtct actagctacg caagtggtgt agcttttatg
    14341 gcactggatg attcttttac aagtaaagtt ttagtggcat cacacaaaaa cgctacaatt
    14401 aaaggctatg atgaaaaaat ctcagcatca tcactttggg ggcaattgat tgctaataat
    14461 gcaatggcaa atcataaacg aacttttcat attgtcttaa ataaatttga ttactttggt
    14521 gatccaactg gtagaacatt tgttgccaaa ccttcgttaa acagtgtaat agaaaaggtt
    14581 aatttggctg aagaaggtct taactctttg gagctttttg gtaatcaaaa ggacattggt
    14641 gatttatata gacggaatct cgataaacta actcagattt tttatttgtt agcaggcaga
    14701 acattagatt ctgcaactag agttttattg aaaaaagaat taggtaactt ttatataagt
    14761 cagaaacttt gggatattaa ttatgaagaa cgttcaaaac ttttggtgaa cagtagtgat
    14821 acaatgccag ttcttgggga ttttattagc tatttgacta atgctgaaac aaaagccaaa
    14881 aaatctggta ctgaaaaaga agtcgatcgt attattgtat taaaagacac attgagaaca
    14941 gcattagaca gcttctctag gatgtttaat caaagaacga cactgaaaga aatcggtaaa
    15001 agtgacaaat tacagtttta ctataactta ggtgatctaa gagcagaaac caatgttatg
    15061 gaagctcaat ttttaaatac ctttgatttc atcacatcac aagcaaaaaa agatgatatt
    15121 gttatgattc acggcttcga taagttatct attgaaaccg ttgggctaat taaaaaaaga
    15181 ttaacaatgc ttgaagaaga tggaattcgt cttgcctttc tatttgatac aatcggttca
    15241 ggagaagaca atgaatacaa agaagatatt gaaacagctg atatattcaa tacagatgga
    15301 aaaatttacc aaagttttga aagtgatttt aattacacta tcattggaaa gatgaccagt
    15361 ggagagttaa tgaagtatga aaaaaaagtt gctcctgcta gaaaaggaga agatgatcaa
    15421 aaactttctg ttgatttgag acagtatttg acaacaggaa gaagtaataa atttcagatc
    15481 aggagaccag tggatctgac taataatttt gttattggag agtttattct ttaagtttta
    15541 tctttgattt tgagtgtata aggaaaggat aagaaaaaac atgtatattt gagactaaaa
    15601 acagtcttag agaacatggt acgttttgtg aaaagagaac tatagaaatt tcataaagtg
    15661 aaaccttatt gagtgaaata attatagtgg aggttaaata atgaaaccaa acataaaaat
    15721 tttagatcga atatttctag gtagagacac agaagtaatc ctaattcaac atgaagaagg
    15781 atttgaagtt agtataggta ttcagaaact tcaaaaacct cattattgta atcagttgta
    15841 taaaaacttt actgatgaag aaaaagcgag agtttttttt aatgaagtca aaggtatgag
    15901 agaacagtat gaggtggttg aagcatgaat aagttaccag aaaagaaccg tgtatttaga
    15961 ttgaaaggtg atctcagtca atttaaaata gatgagaatg gacaattttt tgtttggatg
    16021 cgacatgagc attgttggtg taatcctttt gtgttaaaaa aagaaaatga aatcagaact
    16081 aaaattgcaa atggtgagtg ggattacctc ttcagatggg aagatgaatc ttttatagca
    16141 aatgatatgg atcatgcatt tgtctcattt ctggataata catggggtac ttggtacgat
    16201 tgttacctag aaaaattggg ctttcaaaat attgatattg atgctattga atgtcttaaa
    16261 gaagtcgatc aaaagtatga agaagaaccc tattatctct tgcaacaaaa attagccaat
    16321 aattcagtgt ttcaggtaga aatttgggaa aatgaagatg gaacttatga ggggctagcg
    16381 caagaaatac ccttctcaga taggaagagt tcgatttgaa aagagtatat cagcaatttt
    16441 tatgaagtga aagcaagctt acaatatagt aatggaaaat tgtggttagc tggaaggtat
    16501 attccagcta accaaatctt acgttttctg ggagagtggc gttttaaaac aatttcctag
    16561 gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa tgaatggaaa
    16621 gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg cttagagaag
    16681 gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc agaccaattt
    16741 ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa tgatggaaaa
    16801 tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac atgggaaatg
    16861 agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat ttcacacacc
    16921 gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat ccttttttca
    16981 atgaaatgtt ttccgccatt tcattgtttg gtgcgatttc tattttatta tatcaaaata
    17041 aagagagatt ctaaagaaag aagagggaaa tatataaatc aagcgacagt atatttatta
    17101 ttaaatgcta ttgtgagaat ataactaatt agtttaattt gaaagtaaat atacgctata
    17161 aaagtgcatc ttgtgttata aaaaattatt ttattttgtg aacataattt aacaattctt
    17221 tctcggaatt gatttctttt cttaaatagt cttcagactt attaataata ttatctatta
    17281 tgtctagata catatcactt ttcgattcag ctaattcata cagttttttt aattctttat
    17341 cctttaattg aaaaccctga tccatagtca ctgcatttgg atatagaagc ccactataat
    17401 gtttacgtaa attcacttgg aatttctgat tataaaagtc taacagatta gaaataaaag
    17461 gcggaaaatt actgtttttg taaaattgat tatttccatt taggttgtat agtaatttgt
    17521 taaattgctc ctgatttaat ttattgtaaa tccatggtaa tacttccata tccttatctg
    17581 ctaacctgaa tgaactagca tcggtaagat gttcgatcat aaaatctatg aacatctctt
    17641 tttcaccaaa atataaatat tgattatata ttgtttctaa tgttgaagat ggtagcgaag
    17701 aaactctcat tttttgctgg agtataggat caactagact atttaagtga ttttgtaatt
    17761 cttcattgtt gtttcctaga actgcaaggg aacgtaaacg gtagtaatat tctatttctg
    17821 ttagtacttg ttgtttcggt ttaaaatcat ttattgatat ttgtaaattt tgaattgtct
    17881 cgtctgaaaa atcatgttgt aattctggat gggtagctat aaaagtaagt aaaaagaacc
    17941 cttttgtata cgtgatctgt tgattttcac caagtgattt attaatttcc tctcttgatt
    18001 cagctgtgaa cctatacttt gcaatttctt tttttagaat atttaaatag aattctttat
    18061 tatcaaaaat tagggcatta agtagtctga aagtactttc cctatttcta tctgcttcct
    18121 catttccata agataattga aaaacaaact taaaggctgt cttacatagg ttttctttgg
    18181 ctttttcgct tagtttattt agatagctat tttttagtgc agagtgaaat tcttcaggtg
    18241 taacagacat agcatgtatg cgacttgcat attgtataat ttcgatcgtt actttttttc
    18301 ctaatattgg attttttagg aatataattt cataggactt ccgtataaca tcctgacaag
    18361 tttcttttgt tatatctctt aattcccaag tattaccgtt gtaagtgact acaggatgag
    18421 ctgaataatt tctttgatcc ttaatatatt ttaaatcctc atattcatag ctatcaatta
    18481 gctcagttga actataagct ttttctagta gttccatttc ccattttgga gagtttggat
    18541 cactttttct tagtctagct atatcttcta aaattttctt agaagtatta tcctcgtaaa
    18601 tatttgctaa atcagataat ttatcaatta aatctaaaat aagcgcactg taattgacag
    18661 ctattgcaga acgataattt ccctgataaa aactacttct aacttcttct aataatggaa
    18721 taattctagg atttttaact ttttttattt catcatctaa tataaacata taaatctcct
    18781 ttttttattt gtatgacaag tttaccataa aaaaaaatta aaaagcgaga catatttatg
    18841 ttgttgtgtt atcataataa cataaatatg tagagtgaga tgatgaaaaa atggatttga
    18901 aacaatcgtt ttacatctgg ttatatcctg ataatcctat gtcaataatg gctgatattt
    18961 ttgtagttag catcttattg tatttatttt atctaatttt ctttcacaat ttaatcgggt
    19021 acatgattct tgattggaaa ggaatgttcg atatagaaag aaaaccattt gctaacaatc
    19081 aaacctatac cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat gtgatccatg
    19141 aaattggatt agctttatca ggaatacaag atgctgatgt tttggtatca atgacaacgg
    19201 atactggata caacgcttat gaggtatttt atcttatgta ttttatatgg cgtgatggtc
    19261 aagaaattat caatggtcgt gctgaaaaaa caatcaattc taatactgaa atttcttttt
    19321 gtatgggtga aggggaaatg attgtatata aagatgggtc agcaaacatt aaaacagcct
    19381 ttggtgatat tgaaacaagt attaaggaac ttagggaata tgtgcagaac ccatataatt
    19441 ttcaatattt agcaggaaga ataaatttag tttaggagta aaaatatgaa aaaaattata
    19501 aatcaaccaa caaatcactt gataattgaa ggtgttattt tcgtaacaat gtattttact
    19561 gtaggaaatg gcatctcaaa tacaatcatt caaaaaaatc cacaatacct attatttagt
    19621 ctttatgctt ggattgtaca aatggtttgg gtttccatat tgatgctgtt gttttattgg
    19681 atagtacggg ttattctttt attgttaaac aaaagaaaaa aggataggtg aataaaatga
    19741 aatcaactaa attgaaggtt gtattgttat cgacagcagc attaataact cttacagcat
    19801 gtggtacaac taatagtgct gataataaca aagacacaaa gtctagtagt acggtggtta
    19861 ctaaaaacag ctctacatcg gcatcaacaa caatttcttc aatgactgaa aataatgctg
    19921 aaaagtatca aaaatacgaa gacattaata aaggtcaaaa gccggaagaa gtttataccg
    19981 actttattaa tacagtaaaa aatgatgaag attatgggaa tgatccattg caaaataaaa
    20041 atctgactat cgttctttac aaacctgatt gtccagattg tcaaaaagca gaatcaaaaa
    20101 tcatgcaaga aataaaagaa ggatttagtt atgctccaac attaatgcat gatattacca
    20161 taaataaacc agcttacgag tatcacgcag ttgctgtaga tattactgat ggtgtacctg
    20221 agtggataca aaacattcct ggtctggctg atgagaacaa tcaatatcat acgccaacag
    20281 ttgcagtagt aacaccaaag aaagttaatg gcacactata ctgggatgta atgggaatga
    20341 caacaaatac aactgatgaa atgattgaaa gtacaactaa atattatatg gatttgggag
    20401 catataaatt atatcaacag tcagaacctg ttatggacga tcaatccaaa gttaaatagt
    20461 tctttagggc gtaagcccta tttttttaaa attaagtgat aacaaataaa tgatttctga
    20521 aacaaacgtt cttactcaac tactcgaaac acacaagcta attaactaga ttataaagga
    20581 gaatgcgtaa tgaaagaaat cagtgcaaat gaagctcata ttccgcaaaa gaatcaggtt
    20641 ggtatatata aggtgcttca tggtgaagga atttataaaa tacagttact taccggttac
    20701 aataacggtg caaatgggaa gtggttaaag gcaaaaagta ttcgtgaact taaaactgaa
    20761 gtagaaaaaa taagacttgg gttaacaagg gaagaaataa gtagactgag tgaaaattct
    20821 tcttttgtaa tccaaaaaga aaaggagaaa catctacttc atcgggaact aataattgaa
    20881 aaatactacg atgttgattt acctgatcag ttaagaatta ttgacgaatt ttcagagaaa
    20941 aagtggaaat ctgaaatata cccaagctta attcaataca aggcatcagg aaagcccatg
    21001 tataactaca caggcttttt aaactacaca caatataaat atgcctttcg caaattaaaa
    21061 ggcattaaac aagctgacat tgccctgtat gagatgaaaa aaatggatag attgaaattg
    21121 attcaagagt tagtgcaaaa tacatggaat caagcgattg tcgaactaga aaaagaaaat
    21181 gggaaaattt taactaatga atatctgatc gtaaatgaaa ttgaagaatt aattggttta
    21241 ttttcatgtg aaaatggtga agtcttcttg aagtcatcac caatctattc aattttggac
    21301 agtccgtatc agtttataca ggtaaggaga tgagttacat gtctagcttg aaagatgact
    21361 atatccaagc aaaacaagag tatgaaaaaa tacagatgtt aaaatctgag aaagtcgaat
    21421 tatttttaac acaaaaatta gcagaggatc atattgctaa cttagatgct aaaactttag
    21481 aggaatatga tcagcagttt aatcagacac atcacaatat cattttgctt gttgatcagg
    21541 caaagagaag acttgaaaaa gcggaatatg aactattaga tcaaggaatt atatgcttgg
    21601 ataaagggat tcgaagatat tatagtgcag agttaaaaaa gcaaaatgta gacagacatg
    21661 cgttaaaaca tgaaatctta gaattgataa tgcaacgaat ttgaagggat agaaagtaaa
    21721 tgtcacaaaa cattagtgct gtaaaacaat taatagaaaa agatttagag acgacagttc
    21781 agaggttagc cgaacgaatc cattttgcaa attatcaaac catctctaat tggtcaagca
    21841 ggaacagaaa tccggaagaa attccaatga aattttttta tcgaatcgga aaagaatatg
    21901 gtgttgatcc gataaaaatt ttcgaaaaga ccatgattta ttactatcat ggaacaccta
    21961 acgaagtgtt ggaacatctt ttggaagaag cagaagaaaa atcatcaata ttagagatat
    22021 cacaaaatga caaagctatt caagtgaaat gtgttgatcc aacaggaaag attattgttg
    22081 gatttgatat tgaaagaaaa tctaaaatag attttattac tcaattaaca aagataaata
    22141 atttaaatat tttaacactt agaccagatc atgaacagtt gtttatgagt tttaaaaaac
    22201 aattgaaaga aatacaaaaa agatataaca aggtgttttc taatgcgtga aaagctaaaa
    22261 gaaatcagag tgttttggat aattaacaaa gaatttatta tcttgtatac acttggattt
    22321 atcagcatga tcttgttttg gtggttatat ttaaaaaagt aatttaaaag tgtcaaggag
    22381 atttttatcc agagaaggat gtggaatttg catgtggaat aataaattaa aaacaggctt
    22441 gttattaata gttatttcct gcgctatgat ggttggcatg ataatacaaa gggaacaatc
    22501 ttattttgaa gtttctgcta acaatgtaat agaaagatgc cattatggtc aacatttttg
    22561 gactggggaa gtcagagaaa atatagatcg tgagtatgtt caaagaatcg tttgggatgc
    22621 atattctatc aaagattatc ctaaaagttt aacgagtaga ttattttaca gcgaaaaaga
    22681 taatcaaaaa ctctcgaatt tgatgatgaa aaaagtaagg aaacttgcac aaaactattt
    22741 ggaagaaaaa gaaggtaata gtccaattga caaaaattga tgacactgca aaaaaatata
    22801 gagttcgaat tagctttttt cggagcattg ttttttaggt gaactatatt tcatcactaa
    22861 aatcagagat aactaacttg aaacatggtc agatgtgtta cttgaagtct ctgaataatc
    22921 aatgttatct agtagaatta agcaactaaa aaagaaagaa gtaagaaaat atggcatacg
    22981 aaaatttaca attagtagtc gcagaagtta gtggggatat ataccttaca aaagtaaaag
    23041 atggaattat ggacacgaat tataggagag ttgctacaga tgatgtgcta agctcatcga
    23101 cagaatggtt tcgaaaaaat gaagaatctg ctgttcactt tgaaggaatt gatggaaaca
    23161 ttcatagttt gttctataca aaagacaaag aaaaagcaaa aaaaattctt gatattttga
    23221 aaggaaggta gttaattaac caatgatata agagaatgtg agaccaagta aatacttggt
    23281 ctctataatt ttagccacta gtcaattaaa tagctaaaca attttaataa taataaaata
    23341 tgactgcttc ataattaaga agtgcagaat ttttctaaga acgatatttc cttgttgaaa
    23401 tgagtattgc taacaatttg atcacgaaca gcatcgttac aaatgttgta tcaatatttg
    23461 atagattaca tattctatta aaaaagaaaa gaaaattatt gatattatac acattagtgt
    23521 gtataataaa agagagggag tgattagaac tttgagcaat tcactaattg tttaataata
    23581 gaacgattcc taagaagttc attgttcatt ggcaaattgc tttatcagat tgaaagttgg
    23641 cgaataaggt ttgtagaata acaccttttc aagcaaagcg atttttgaaa gctatgaaat
    23701 ttgtttaata aaaaaattca ccagataatc tgatagagac taacccaatt actaacagtt
    23761 gtaattggtg ttagcttaca aattaattat tgtgtttagt caattacaaa ttgtgaaacg
    23821 gcactaataa ttggaaagga aaaatgaaat gtctaaagat ttacgagagc ttaaattgcg
    23881 aaaaaagcaa tataatcaat atttttctag tttgttgaat aaacatattg aagaaggact
    23941 tgatcatgat tttggaaaag atgaatattg tttatattgc gaacgttaca atgaaaaaat
    24001 gtctgaatat ctacagctga aaaaagagat acagaaatta gagaaaaaag tacatgcttg
    24061 gaagtataaa gattccaggt ctggttatgt tgaatctcaa aataagccaa gaaaaaagaa
    24121 gcaagcgtat gttctaaaga tggtaaatgg ggaagagtta attgtattag cgtacagtga
    24181 agaagaggtt cgagaactcg ttgaaacacc tgtcaggaaa aaggttgaaa caataaatga
    24241 gatagacgac agccaataca atagcatcta tattgaaaca agtcatggtg gatataagaa
    24301 attagcaact ttccttagaa gttctaaagt tgggataatt tctaaaaaag acataagtgc
    24361 tgttaccgat agtttttgta ctttatctgt tcctaaagct tatttccaat acgatagtaa
    24421 aagatttgaa cgcttagaga aattctgtag accgtatact gtattgaaaa ctaaaaaatt
    24481 taataaaact aaaagagttt cgatacagtt accgactggt caaatctcat taggtgaaaa
    24541 agactatctg ataaaagtag gaaaagagtt aattataaaa gtaccggaac acttatttaa
    24601 agaaaatttt gagatacgtg aaaccttggt atggaaagaa gaaaaatagt ttggaagaca
    24661 tagaatggta taaggggaaa ttgaaatggc agtaaataaa atattgcagg tattaatcat
    24721 agacgaattt ggcggaggag aatggtttga tgtgcagtcc atagactttg aagaagaagt
    24781 cgtagttata gaaaatattt acggcgaagt atactcatat tcgcttgaag gtgagaatgt
    24841 tgaggatcta cggttgattg gtgagtagaa aaaagcagaa atatcaaaat ggagataaaa
    24901 attaatagca tgtaaagata gtcatgctta catgctttat ccaaaatggt atgcagacaa
    24961 aaatatagct gaaataaacg attatctaac tagtataaag gaacgcaatg caattaatta
    25021 acaatagacg aaaaatgaaa ttaaaggaga taggaaaatg gtagaagttg aatttggatc
    25081 aaaagaatgg tttgaagagt taaactcaat gattaagttc ccagaaaagt ttacttttta
    25141 ctacaataaa agagacaata tcattcaagt agctttactt gaaactaagg gaagattaga
    25201 aaatactcga tggagtatga taaaaccatc ggcacttaga tttcatccta aagacttgac
    25261 agcggttgta gcggtaaaag aggttcaaga ctcacaagaa tattatgaag tagtaaaaaa
    25321 tctgaaggat atttggtgta aataattcta aattgaccag ttatttggtc aaaaatagta
    25381 tgtgattatt tcatccaatg tggcatcata attggtatgg aaggggataa attatttgaa
    25441 agaaatagaa ggtattaaga aaagaaatca ggaaagattg gaactgttaa atgaaaatag
    25501 aaatagaatg tttagttatt atgaatctat gaggtgttta aataaatcta gaagtgaaat
    25561 tatagaatca tcaaagtata aacaattgat ggaagagaga gtttctctca tccaagaaat
    25621 agaaacagag tctatttttc cagatcattc tcctaaagtc cgattttata attaaacatt
    25681 aacaaatgaa acgatgctaa ataggcatcg ttttttatct atttataaaa ttaatttatt
    25741 tacaaaatgt gtatcggtat gtatggtgaa tttgtagata agttgagaaa gggtgatctt
    25801 aaatgaaatt agaaggtagc ttacaaaata gaatgatgga aggcagtaca acaaacaagg
    25861 agattgtagt tggaatggga gcaaccgagc ttctatatac cgacagaaat ccttatacag
    25921 taattgaagt aaaaagcaaa aacagaattt tggttcaagc ggatggagca atcaataaag
    25981 ctacttttcc agatcaagag tgggaatata gcagaaatcc tgaaggtcaa atattggagt
    26041 tagttaagac aaaaaatgga tggaaagtgc ttaaagaaga tattcatttt tatataggtg
    26101 atagagaaaa atactacgat cctagttttt aaacaacctg aagatgagcg aggttttatc
    26161 ctcgctcaga aaggagtatg gaggatgttc agtcaagaag aaatcatcag caaatcgcaa
    26221 ccggataaaa atggaactat aatcatgaag aaaaaaatcc cttgctatcg ttgtagcgga
    26281 tcaggtcagt acaagtttat gtctatgggg aaaattacct atggcacatg ttttaagtgc
    26341 gatggtgatg gatttcttgt tgtaactgaa aaatccttta cgccagaata tagaactaag
    26401 ctaaatgttt taaataaaaa aagagctgat cgaaaaataa ggatcgcaag agagaaagaa
    26461 tctcagaaaa attttgatcg ttatggcttt gaaaagggtt atatttacgt ggttatagag
    26521 aaaaatagct atacgatcaa agatgaactg catcatcaag gagctactta tgatccttta
    26581 ttaggttggt ttttttaagg aaaaacaaga acaatacaaa actcaaaaag tttttgtaga
    26641 agaattttct gtaatcaatg atgaaggaat tattgagttg aagctagaag aatacagcaa
    26701 aagacaacag gagattttaa gtgcgaagcg tagagaagag ggtgagttta ttggtacaat
    26761 cgctcaacga ttggaattgg atttgaaact taccaatacc gtcaccatag atacacaatg
    26821 gggcaatgtt tacctgcatt tatttgaaga tcaaaaccat aattcattga tttggaagac
    26881 aaataaattt ctttattatg atgaatggga tgttggagaa ataagaaaag ttaaggcgac
    26941 aataaaagcg catgactact acagagatac caagcagact tggattaaaa atgttaaaat
    27001 tcaaaaaaat gagtaagaag ggataaaagt atgaaaagag aagaatacaa acaaagatta
    27061 aacgaactgc ttgaagagga cgaaacatta actcatggtt cgccggatga aattctatac
    27121 atgattgata atatggtaat ttttggtgga tatgaacttg gtaataggtc agttgatcat
    27181 aatattttag aatttgatga tgtatcatgg gaagaaattc ttgattgggg aattttggca
    27241 gtacctgaaa ctaaaaccta tattagtgac actatggttc ctttttttga ggaattggat
    27301 tataaaagac ttcccaaaaa tgaaaatcat atactgggag gaaactaaca atgttaagtg
    27361 acttacttat tttactgatc tatctaggtg gaactgaaat tattgcgtat tttttgcttg
    27421 taattggtga atatggtatt tccagtgcaa tcactcaaag gtttagatta ccagtaagtg
    27481 aactaaaaaa atgatgagat tatcagcata tcttttaatg tttttgaatg tggtcatgtt
    27541 aatctttgta gtaatcggtg tgactttcaa tttaacggaa tagagggaaa tctataatgg
    27601 aaaaaagaga aaagaatgaa catatttttt gagtcaatat tgttgatggt ttttgcgatc
    27661 agcgcttacc agaaatctaa aaaagagtaa tgactgttta gtcatcactc tttttttgta
    27721 gggaaattga aatatgtgga gcttgtattg gtttagttag gattgcttgg ctataaggag
    27781 ctagttcttt atcaaaacca tcaaagtagg ttttcagtag cgatttaact gatcgctcat
    27841 cttgtttgat ttggataatt tcagcatcca aatcggttgt gaggtcagga gcttttgaag
    27901 cgattacctg atttttttta tcttccaact ttcttaggct gtttaacaag ttgtaaaaat
    27961 aatcattttg actgttcatt ccaacgtgag atgcaatttt aacagaagtt tcatccgtat
    28021 tggcatcaag tatttcatca aaagaactca ttggaatatc aagtagtaac gaggatgttt
    28081 cctttacagc atttttagtt tttccatctt ttttgctctt aacttgagat atttttggat
    28141 ctagtttagg taaatcactt gttttatcag tcatttatta gcttttcctt tctattaatt
    28201 tatgttatta tattatcata ataacataaa aaagaacact ttttgggggc gtagttattg
    28261 aaaaaatcta agagaaagat tgccacgttg cttacgttaa tggtgcagtt tacagggatt
    28321 gcaatgagtg taatcacaat aaatccgcaa gtgttacatg ctgatgggat agctacttat
    28381 gatgcacaat ttaactgtac tactgaaggt agtgggtcag gaacagatag cgatggaaac
    28441 gatactggaa gtggcgctgt aacaggtgat gtagccaaaa atattgaaga tatttacaaa
    28501 tacatgaatg gagaatatgg cttatcaggt gctagtattg caggtatttt aggtaactgg
    28561 aaacaagaat caggtattga tcctcgaaca gtagaagggt attacggcga tgggcagaga
    28621 gaagttgact tcatcaaaca atttactgat ggtctaatgg gtattggcta tggtcaatgg
    28681 acagccgggc gacatacagc attagttgat tggtctaaga aaaataatgg cggttcttgg
    28741 tggactacag aagcccaatt ggactttatg ttcaaaggtg atgggggatt cgtaggaata
    28801 ttaaaagatt atgctttaaa tgcttctgat gatgtagtag aaaatgcagt aacttttcat
    28861 aaaaactggg aaatttcagc tgattccgaa tcagctgtaa tagctgtacg tggtgaaaat
    28921 gcaaaagaaa tctggaagta catgaaaagc aagggtatgg acggtgcaaa agatgaaagt
    28981 aagatcaata aaatatccgg aggttcaagt accggagatg gatcgaataa ttcatcaagt
    29041 tcagcatcag gaacagtaga agatgcctgt gcaactacgg atgatgctaa ttctggttct
    29101 ggatcaattg ggaaagaagg aaaaattaac ggtaaaaaag gtcaagtaat agcaaatatg
    29161 acttatgaag aagcattaaa aaaatatggg aaagcaatca cgctaccaaa atttaaagaa
    29221 ccagactggt caacatcacc ttttcaaggt gaattacaag gacaatgtac tgaattaaca
    29281 tgggcttata tgagtcaatt atatgatgga aatcaaccaa ctttgggaaa tggcggttac
    29341 gtttgggaaa gctacaaatc ggcaggagca aaggtgacga acaaaccaat tgtaggttat
    29401 gcgtttagtg ctttagatgg atggttatgg gctgttggat cagcaggaca tacaggtgta
    29461 gttatagcgg tgtttgacga tggatcattt ttgtgcgcta atttcaatgt tccacctacc
    29521 caagcaccaa caagaggtgt ttcagtaact ttgattgatg gtgtggatgg gtcagataat
    29581 gtgcatttct ttagcggtgt aggcaaagct aaatttgaaa ttgaaaaata aaaatttgaa
    29641 tggagggcga cagagatgaa tagtaaaacg aaagatgcaa ttatcatttc tttgctttct
    29701 ggtatgctat tagcaatgct tgttggaatt ctatatttca ataagcagaa tcaagacata
    29761 gaagctgaaa agcacagtgt gcaaattgaa aatgaattga ccttctttaa agaggttaaa
    29821 ccaaatgaag tggatggatt gcaaagagat ttagaaaatg atattcaaga gatgtttaaa
    29881 ggatcagacc gaagcaaatt tgcgaatcag tcaggtgaag aatttttgcg aaagttattt
    29941 attgtgacag gtaagccacc agttactaac gagagtagcg agcaagaaaa aatagaatac
    30001 tataaacctt ttacttttaa gctaacaaac tttggaatta agacatctag tagtgggttg
    30061 tatgaagcat tgactgattt cgaagttcaa tacaacggaa aaaaagtgta tgataccaca
    30121 ttaaccatca cactaaataa tgataagaaa gtgactgggg gtgcttttta tgcaaaatca
    30181 tgaaattact actgctaaaa aggcgatcgg aatactttcc ataattttga tcctttgttg
    30241 tgtggtacta tttcaaaaga aaaatgctta tgataataat gtcaaagaat tggaagacct
    30301 taaaagtaaa caaatcgaag tagtcaagaa taaccagaaa gtgaaagcta tgcaagaagc
    30361 ccaatacaaa gaattgggaa atgctgaaat caaggaacaa agtaggaaat tctttaattt
    30421 attttacaac tggaattcat gggaaaaata tacgaagaat atgaaggaaa tacaaaaaga
    30481 atttcctcag atagtaaagt ctagtcctgt tgatatctct ggcacaatgg taggtactgg
    30541 taatagtcca atatctagct atactgctga ttactataca acaaatcaaa aaggtaaaat
    30601 ggttgaattg attacacaga caagaaatac tccaacaaca gcaacaacaa caatttggcg
    30661 tggagtaaca agcataggag aaaatcaatt gttttacttg gaggatttag atagctatgt
    30721 aagagcagag taaaataaaa aaagagctaa cggtaccaac gttagctctt tgtgctgacg
    30781 atgataacaa aacctaccga aaaaggattg tttcatcaca aacacaaaca gaaaagaatt
    30841 gataaaaaac ccgaacctca gaatttttat taatcaattc attttgatta taataaattg
    30901 agctgaacat tacaatacgt atatgttgta aaacgtaaaa aaagtcaaaa gtaaatactt
    30961 tcttaaaaag tcaaataaaa ctattaaaga caatatatat ggtttatttg acataagaaa
    31021 atggtctaga tgataattag aatatagaat tgtagactta aataaatcat aaaaaacctt
    31081 ctatgataag ttatagctct taattttaag cagttttaga tataagaaag taaagtgggg
    31141 gagaataata atggcagatc gtaaattaga aaagttatta gaagaaacgt gggatccaaa
    31201 agaatttagt gaatttttta tggagaattt tgaaactgac ttagcggtga ttgttaaaga
    31261 tgctcttaga gagcaagggt atcctgaaac tgcaaattat ataaacatta attttacttt
    31321 gtatactgaa aatagaggaa cttgggattt ttgggctaac ttagcaaata aggagttaag
    31381 tgataaatca gatacaggaa ttcgtaactt ttttgaatca aacagagatg attatatgta
    31441 cgctaacaat caaaataaat tgaactttag agtagagttt gatgaaacac ctgaagaatt
    31501 tatagaaaga caaccgccta aagaaaatgt agcaaaggta ttggaagatc gttggaattc
    31561 agatgaaata gtttcaacaa ttagcgaaca agacggacaa tatgaaccgc tagtagaagc
    31621 ggttagagaa gagctaagac tgaataagtt tcctgatgtt caaaatattg atgtttctca
    31681 aattgaaata aacgtcaata tcaccaatag acttgattat gatagttggg cagatatagc
    31741 attagaaaaa tatatttact caacgttaaa agagtttatc gaaaatagaa tgaatatcat
    31801 gtatctacag catccacagt atttaaactt tggtgttgaa atagctacac ctcttgaaga
    31861 atggaaaatg gagcaagggc ttgactagcc tttgctccat ttttttattt tacaaatgaa
    31921 tacagttctt cgtctttacc aagaatgatt gtttgcccaa ctttgagatt atcaggtgat
    31981 ccttcagcgg tttgtaagga gaacgttcca ttgaacttat cggataccgt tataggtagc
    32041 tttaattctg atccttcctc atctttgaga attgctgttt tccgttcatc aaaataaaca
    32101 gtctttatta gataattttt agtagtggtt tcatcagatt tgtctttgtt tttagtagta
    32161 ctaatttctt tagaattaga tgtattttct atgactattt cgtttttggt atttgcgctt
    32221 gtatttattt gattgaaaat tttaatcgac aagatcccac taagaatagc caacatagaa
    32281 acaagtgtaa ttaatactga taatttcctt tttttgttca tttcttctta acttcctttt
    32341 ttgttttgat tagttagata ttccttatct aataagcctt tttaggctat taaatgaaga
    32401 taaaccccaa aaaattattg caataatgat agtgcaatgg tactattatt ataaaataaa
    32461 ttttgcgaaa ggggtaggtc taaatcgcaa ataaaaagaa attgaaccta gacaaataca
    32521 acttgtatac cgcaagggaa gcaagtctaa ttttgggtaa aggtagaaat tatgttacaa
    32581 acataatcaa ctattatcca aatcgtttgc ctaaaggttc gttcaagaaa gtgggtgaca
    32641 aaacctatat tataacggaa gaaggaatag aagcattgcg atcacaaaca gaaaataaaa
    32701 acaaaaagaa ggaatgaata tgaagaaaaa ttcgcttgct atggttttat ttgcatcagc
    32761 tgtacttgcg gggttatcag taccagtaac ctcacatgct gatgaatgga ctgcaaacac
    32821 accggatcaa atccaaatta caacaggaca atcaagttat gcgttcaaaa aaggagatac
    32881 tatttggtca actgtagaga agcttaaaag cttaggttac aagactactg ttgctgagtt
    32941 tgccgaattg aacaatgttg atttggcaaa aggagaacaa cggactatcc cgattggacg
    33001 aactttcaac atcaataaag atacattaac agttaaggaa gcagatggcg aagtagttag
    33061 tcaaactgtt caaaaagata aacctgatcc tgttgaaaat acggagcaag agaacaaaaa
    33121 atccgagact acaaaagaat cggaaccaac agcaaacact tctgataaga cagaaacaag
    33181 tgcttcaagt gttgatggag caagttcatc taatacaaat aattctacaa gtgagaaaga
    33241 agcatcacag ggaacaagtg aaagtagcac tacggaagct gatactggaa catcaacaaa
    33301 taactcacaa tcaacatcaa atgatgcaaa tgaatcaacc agtacatctt ctagtgatca
    33361 atcacaaaca ggttcaagtg atgaaactga cagttctact tcaccaagta accccgaaca
    33421 accaactaat gttgtgctag gcgttccatt tattagtcaa ggtaacacaa tgctttgcga
    33481 aggaacatct ttgctagaag cgttacatta taaaggtgtt gcaacaaatc aagatttaat
    33541 gacttttgtt aaatcaatgc cactatctcc tgataataat ccaaataatg gtttttctgg
    33601 tgaatggaga cacaatgtgg atggaactta tcaaggtatg aatccggctc ctgtagtttc
    33661 atggggtgtt gctaatggcg gaaatgttgt agatatttct ggttcatcgg tacaaggatt
    33721 aaaagatgag attgtaaatg gaaatcctgt agtagcgtgg gtaacttatg cctttgaacc
    33781 tgctcaacat aaatcaatgt tttggggcga tgctatttgg aatcgacatg tagtagccgt
    33841 tgatggattt aaagacggtt tttaccatgt tgtcgatcct gtgtttgggg caagttggat
    33901 taatgcagga tcatttgaaa cagcatataa cgtatctcaa ttagcggttt cggtacgtta
    33961 ataaaagaga tcgtttatat actaagtagt taagtatagt ctttatttgt ccttgcttaa
    34021 ctacttagaa aacttgttgc tcatggttgg tagaactaaa aggggtgatg ctatgagtac
    34081 tagagcgttg aaacagcttg ctttcaacga aaaaagtgat taataggtgt ggtgatttta
    34141 acttaggaat gggcgcgaag gtcattacca caccgacata tcgtggctca ttttcttcgc
    34201 ctaactggta taaagaagga gtaacgagag atgaagcatg aaaaaacgat aattttaaaa
    34261 tcaaaaataa tttattgttt tgcgacttta gtaatggtgt tatctacgct tataatgcct
    34321 ttttcagcaa taagagctga agcagatgtc acaaaaccga caagtgcaaa atttacagaa
    34381 actggcgtaa aaacagacgg ttatattagg gttaatatgc cgaatcatcc aaatgaatgg
    34441 atgatttcaa gtcaatttaa agattctcat ggaaatattg gatattgtat ggattcagaa
    34501 ttgccatccc caacaggaag tggagccgga agcttgaagt acaagggagc tggcagtgat
    34561 gagttctatc gtatgtttaa aggtggtttt ccatcaaaaa cagcaaagga actgggtgca
    34621 ggaaatgata cagaagcatg gtatgcaaca caattagttt catgggtttt agccggaaac
    34681 tttaaagttt cacagattgt atggtcacat ccaaaccata ccgccgcaga aacagctcgt
    34741 gtaaaaaaag catttgaaaa aatttatgac tatgctaaga atggaaaaga tacgcccaac
    34801 actgagtttt ctatcacagc ttctaagaca gctgacgaag gaaaatatca cactttcaca
    34861 tataaaacag catcaaataa gactggtaat gcgaagctca cttttacatc agcaaaacca
    34921 gctggtatga agatttatga tgcagatggt aaagaaatta ctaacaacac tgtgaaatta
    34981 aattcaagtt ttactattaa agtacctgtg actacaccaa gtggtacttt gtcatttaaa
    35041 ggtactgcta atgttagcac aaccaatcct tttacttttg atggacgagg tgtgtatcaa
    35101 gatgccgtag ttatgatcac tacttcagaa acaaaggact ctaaatcttt gagtgctaaa
    35161 tggacaagag ctgttggtca ggtaacagtt aagaaagttg atcaagatgg aaatgcttta
    35221 aaaggagcgg agtttactct aactgacaat aacaatgcta aatctgttaa gacaaccgga
    35281 gatgatgggt tagctacgtt tgatttgcaa caaggcttaa catacagctt agaagaaaca
    35341 aaagttcctg aaggttatca aggttcattt aaaaaggatg gtattactgt taataccgat
    35401 ggacaagaat ttagttacac tgctacgaac acaattaaaa aaggtaaagt aaaagttaaa
    35461 aaggttgacc aagatggaaa accattagct aatgctgaat ttaccttgac ggatgattca
    35521 tcccaaacac aagcagaaaa aacagatgaa aacggacttg caagcttcga tattgtagca
    35581 aacaagacct acagccttga agagaccgga gtaccaactg gttatacagg cgtattcaag
    35641 caagaaggaa ttaccgttaa agatgatgga caaacttttg aatatacagc tacaaacact
    35701 ttgaaaaaag gtttagtaaa agttcataaa gtcgatcaag atggaaaacc tttggcaaat
    35761 gcagagttca ctttaaccga tgaaaaaggt gcaacacaag tagaaaaaac agatgaaaat
    35821 ggacttgcaa gcttcaatat tgtagcaaac aagacgtata gcctagaaga aactggagta
    35881 ccagtaggtt acacaggtgc atttaagcaa gagggtatca ccgttaaaga cgatgggcaa
    35941 acttttgaat atacagctac aaataatttg aaaaaaggtg tagtaaaagt tcataaagtc
    36001 gatcaagatg gaaaaccttt agcagatgca gaattcacct taacggatga aaaaggtgta
    36061 aaagaagtta aaaagacgga tgcaactggt ctggctacgt tcaacattat tgcgaacaaa
    36121 acttacaact tagaagaaac aaaagtacca acaggttaca caggcgcatt caaacaagaa
    36181 ggaattaccg tcaaagatga tggacaagtc tttgaatata ctgctaaaaa cactttgaaa
    36241 aaaggtgtga tcaaagttca taaagtggat gatcaaggga aagcattagc aaacgcagaa
    36301 ttcactttga cagacgacaa aggtgtaaaa gttgtacaaa aaacggacag caatggcttg
    36361 gcaacattta atattgttgc caacaaaacg tacaaaatcg aagaaacaca aaaccctaat
    36421 ggttatacag ggaaatggtc taaagaaggc atcaccgttg ctgacgatga acaaaccttt
    36481 gaatatacag taattaatca ccttgtacct gaaacagtaa gaacaggcgg aatcggtaca
    36541 tcagccttcc ttcttgtatt tctaattgca ggattgagta tggtgctact gtattatcga
    36601 gaacagcaca aagcaaaata aaaaagtgtt aatggaaaag aagtaaatat gaaaaaagaa
    36661 ggctgacgta tgaaatcaaa aaaaattttt tcaaatttaa tgctatttgg tactttgcta
    36721 ggagtgggat tgggcgcaag ctcaatctct tctagccttg gactaggggg gcaagtagca
    36781 tatgcacaac aaaactataa ttatcaagga actggaacga ttcatatcca taaagtaaca
    36841 aatactaatg gggatgggaa ccctgtctca gatattgata acaaagggaa agaaatagct
    36901 ttaccaaatg gtgtaaaacc tcttgaaggc gttgagttct cttatgtgaa acttactaaa
    36961 gatgaagcta aatccttggg atggacggat agtgatacta caaaaggtca acatattgat
    37021 gatgctaaag cacgattatt actaaacaaa aaatacagtg aagtaagtag aatgaagact
    37081 gattcacaag ggaatataga catacctata caagtaaata cagacgattt aacagataac
    37141 ttgtatttga ttgttgaaac aggaacagtc aaaggtgtag ttgaagaagc gtatcctatg
    37201 attgttaacg tacctgaact aaacggatca gatgatcaat actttaatta tgatgtacat
    37261 gtctatccta aaaactattt ccaattaaac tcagcagagc ttgataaaaa agacgaagat
    37321 ggaaaggctc ttgccggaac tgtttttgaa ttgcgaaaag atgatggatc tgatcaggtt
    37381 gttggaactt ataaaacaga cagcacaggt aaattaactg tagatggttt ggaattaggt
    37441 gactattatt tcctagaaac cgacttggga acagatgcta ataaaacaac ctatttacta
    37501 gatggtaaaa aattcaaatt tagcgttaaa cgtggggaga ctgctaaagt agttcttgat
    37561 gttaccaact acaaacgacc tgtaattact aaggtaattg ataaagaaac cgttgatact
    37621 ggcgaagatg ttcaatatac attgaaacca tcaattcctg gtaatattgg taattacgaa
    37681 aaatatgttg tcaaagatgt tctaggtgca gagttaactt atgattcatc agaagtgatc
    37741 gaaggaatga ctttagatac tgattacagt atcgaacgtg acggacaaac gacaaactat
    37801 gtctttactg aatcaggtat ggataagctt cagaaacaat ttgatgcagg ggatcgcttc
    37861 tttacaattt tgatgaacgc tcatactaat agtaaagcta aaatggatac accacttaag
    37921 aatgatgttt caacgatcta caacaatgga tatgaacccg aagacgaaac accagttgat
    37981 cacgcttata caatcattgg tgatcaccaa ttcattaaaa cagatggaga tacaagcaaa
    38041 gcattgagtg gagcaaaatt tgtagtgaag aacaaaattg atccgaataa agttacttac
    38101 ctaaaacaag atccaacaac gaaagctgtt acttggattg cagataaatc taaagctact
    38161 gaattcgttt ccggatcaga cggtaagttc caaattgaag gcttgcaata tacctatgat
    38221 gtagattttg ataaacaagg taatccaaca atcacagata aaaaaatcaa tgattatgct
    38281 cttgaagaag ttgttgctcc ttcaggatat acaccaacta gaaatgatgt tccatttgtg
    38341 gtatcgaatg atgatgcttc tagcgaagta acagacatca agaaccttaa aacacctgaa
    38401 acagttcgta caggtggaat tggaacagta gtatttctag tggttgcagg tgtggcagga
    38461 acagggctat atgttgtcta caaacgagaa caacacaaag cttaatgaaa taaaattccc
    38521 tagagtgttt ttggctctag ggtttctttt taaaaagtgt gtgaaaagta ggtatgagca
    38581 tgaaaaagaa gaatatcaag ttgatcatct taaaaattgg gatgttgcta tgtttaggag
    38641 taggtctata tcctgtaatt actcaggctt atacctacta ccaaactcaa caaacactaa
    38701 actcctataa ggaaaaatat gatgaagctt ctgaaaagga gaatgaagaa gtcttcaaga
    38761 gatattcaga agttaatagg aaacgaaaag acacagaaca aacctcagtc acgattcctg
    38821 aagataataa caatattccg tcaaatgtaa atcttcagaa ggataaatta ggtgaattag
    38881 tgggtgaaat tcttatacca aagattaatg tggatttgcc aatttatgat ggtgtaaacg
    38941 attttcaact atcagcagga gcaggaatat taaccggaac tgattatcca actggtggga
    39001 ttggaaacca ttctgttata tcagctcatt cgggcattcc taataaaaaa tattttacag
    39061 atttagagtt gctcaaactt ggtgataaat tttttattga tatttcaggg aaaaagcttg
    39121 cttatcaagt agatcggata aaaacaatcc taccaaccga atttgaggat ttgcttcctg
    39181 tggcaggaaa agattatgta acacttatga catgtacacc tgttggagat aattctcata
    39241 ggttactcgt tagaggggtt agcatacctt accaaaaaag tattgttgag aaagaaaaga
    39301 agaaagaaga tttaaaggta aaggcgcaga tgaactggta tcctatcgta gttgtagggg
    39361 ttttattaat aatagctgtg atagtcatta ttaaaaaaat agtagcaaag agacgaggaa
    39421 tgaaaaatga agaataaaat actacttttt ttgactctta tttgcacttt ggttttaaca
    39481 atttatcctg caaatcagct ctatcatcaa ttcaaaacaa aagagaatgt ggcggaaatc
    39541 caagagaaaa ttgagaaaaa acaaactcct tcaaaagagg aagtggcaaa aaaagtcgaa
    39601 caaataaaat cgaacagcgg taaccaagga ttagacaata ctcagaaaac agacggcgga
    39661 tcatttgata ttggtgaagc tgaaggaaag gtcaatcctt acacaggaga tgttttttca
    39721 aaaaatcaaa cgaaagattt tgaaccatct acctattcag gggcggttgg ttatgtctat
    39781 attccaaaaa taaatgtgct agagcttttg tttctaggcg cttcacagga acatttatat
    39841 agaggtgtgg cacagatgag tgctactagt ctacctttat caggtaaagg tcatcaaaca
    39901 gtaatagcag gtcatcgtgg cggttatacg tcaccacaat ttctttatgt tgatcaatta
    39961 gtagccggag accacattta tatagattat cttggtgaaa aagcagttta tacggttttc
    40021 cgaacagacg ttattagaaa ttacggtcag aattcaacac tgactgatat tcctgattta
    40081 gatatgttaa cactttatac ttgtcatcca tatcctcaga actacgatcg tttattagtt
    40141 agaagtctaa gagatgaaaa agcaaaatac atcacgccac aagtaacagt tggcaatacc
    40201 gcatacgctg atgccattga tagcgagtcc gtttcagata agacagaaac tgttacatct
    40261 tctaatgatt tatttgagga tgttgatcgt gccaacaagg aattagatgg aaataatgat
    40321 cttaatattc cggaacaagg tagcgtagag atgcaagaca gaacatttgc tgaaaaggca
    40381 agttcaatat tcacaggaat taccaataaa catcagaaaa tgcttttagt catttggact
    40441 gtaataggag cattagcaag tctaaaggtt cttattcttt taatcaaaga gtttcgaaaa
    40501 gcataatgaa gattataaaa attgatagag tacagtaaaa agggattgat actattattg
    40561 atctcttttt tattttgaat agagctagat tcacttttaa ataaaatcaa aactgatttt
    40621 aattctaaag aaaaagtcaa gaaaaaaata caacaaattt ttttatcaga atatttatca
    40681 gaatattatt agaaaagata aactcaaaag cttaatttaa tagggttatt ggttgtattt
    40741 agaaataatg tatggaaaag ctataaagaa atagataaga tgtagaaaat cttatttgtg
    40801 aatgaaaaac aagtcatcgt aaaatattaa taggaggtgt agtaatggtg ggaatatcaa
    40861 aaacagcttt agactcatta gctaaactat ttagtagcag gtttgatagt gcagaatttg
    40921 ttgaatatct aagccaaaac gaaagtggaa aaactctgat cgaaacaatg aaaaaagact
    40981 atttgaatga aaatgacttt ccgacatgga cgaatattga agatgctaca tgggatgtag
    41041 tgtgtacgat atattctcct tgggtaacga ttgctgattt tgttttagat aaaattggag
    41101 gggatacata ccctgatgcc aaatctcttg ctacaagtga ctttaaaacg ttctatggtt
    41161 atctaatagg tctaataggc aatggaatag atttgaaagt tacgccaaaa caaacacctg
    41221 aagaaattga aaaagaaaaa ggagaggttc aaatggctta tgcaaatgac agagaaaaag
    41281 taaaattatt ggaaggtaaa ttatctgaag aagaacttgg aaaaatggtg caagaaatca
    41341 atgaagacag caactatttt cctgaattga aggtatatca aaatgacgaa gatttcttta
    41401 atgaaatgtt tagtaatagt ccatgggaag cagtgagtaa aacaaattta ggagaatgga
    41461 actttcaaga tgatctagtt gcatttagca atgatgggta tttacgatca tattcagaaa
    41521 gtgagtatag agctgaatta aaaacgtatg aaacggatat tctgatgagc tatttaaatc
    41581 tattagagag tggtcaggga gagttaagag ataaagttga agaactatta aaagaagagc
    41641 tatagggatt atttcaacat aaacacaagc agaaaactac caaacaaaac gtaccatgca
    41701 aaaagtcggt taaaccggct ttttgctcta ttatgagagg agatcaaacc atgagtgcaa
    41761 caaccgatat ggctttagaa gcagtaataa aagataatac aggaaatgtc gaaaatgtag
    41821 aagactttag cgatattgca atcaatcttt cagcatggat ttcagctgta ggagaagaaa
    41881 atatcgaatt tgagatcaag aatatggatg acttaaaagc aaaatttcct tttatcgctt
    41941 cgaaactaag tcaatttgcc gaggataaat cgtggaatga attagttagt ttttccgaaa
    42001 aaatttatga tattacgcaa gaagatgagt gtgtttcatg ggaaatgaat aatcaataca
    42061 agaaaattga tgctttcgtc aaatcacgta tagaaggaac agcttttgag tttgaaggta
    42121 tagataccgc agataattgg ttaaataaag aatcctttat taaaagtata gcggatgatc
    42181 ttgaaaaaca atggtctaag gaagactatg cagagtacct aaaaacagaa agagatacag
    42241 aggtgaaaga aaaactcact gaggtatgtt tggatgaaca tattgatccc aatatcgtaa
    42301 aacaagaaga ctatgaagtg gttattgata aagttcctac tgattttgag gaagttgtaa
    42361 agtatatagt tgaagaagca ttatcgcaat actcttcaga tgaggaagtg gacgaagaaa
    42421 ctgttcgtac cagagtttgg caactggttt ttgatgacaa agattataaa tatttgagtg
    42481 atcaaaactc ggttaattgg agtgtacgat tattaattga tcaaaatgat attgaataaa
    42541 atagagctga aaagctctat tttttttgat aaaaaaacta gctatttttt taaaaataat
    42601 ttggttcaaa atttattttt ttaacttgat aattttttgc catccttttt taaattggct
    42661 atatatcaag gtttatagtg gggataaata aaaatacatt tgacaaaatt tacaatatag
    42721 tttattatat atttatctta ttatgataac acaataaaag gaatgatggt atgaaaaaga
    42781 agaagtttag tcgatcgttg ttaggaattt tagttttagt tgcggttggt cttagtggca
    42841 agggttactt tcagaataac agcactcagg aacatgattt tcagatcaaa agtagtgttc
    42901 aggtaggtaa taaagcagac agtgaatcaa aagaattttt agggatacgt tatgatccaa
    42961 acaaatatcc aaataatatc gtaaacatta atgatgggaa atatacactg acaaaagaag
    43021 accatgaagc attatcagaa aaaggcagtc aaaccttttg ggtaaattat gcagatttag
    43081 atcaacttgg aagagcagga gatgttgaag cgttgatcac ctatgatgct ataaattctc
    43141 actcatctaa aaatgtggaa cgtccatctt tcaaaagttc tgtccatatt gcaggagaat
    43201 acaaggatgg agtatttgat caagagagag atacttgggt cggcaaagat tcaaacaatg
    43261 aaatcactca gctagaagga tataaaggtt atctctataa caaaagtcat tcattagcat
    43321 ggtcgctagg aggagatatg gaggtacaca atttaacact tggaacgaga gcgcaaaatg
    43381 ttgggaaaaa tgatcagaat ggagggatgg cttactcaga aacgattgta cgtgatgcaa
    43441 tctataaaaa taagaatgaa aaagtattgt ataaagttga tccaatttat aaagggaatg
    43501 aattggttcc tcgtggttct catgtcgaag ctttctcagt taacgataat ggaaaaacgg
    43561 taaaccttaa tgtttatgta tttaacaatc aaaaaggaat caacatcaac tatcaaaatg
    43621 gtacttgggt aagagattag aaattgagaa ggtgaagaat tggaaacaat aagcatcaat
    43681 ggattagata aatcagttgc aaatcgaata aaaaaaggaa atggctacta tcttctgaag
    43741 aagggtaaat attcagttgc tagagagtta gaaacaatga gtatcgtgta tgtagctaag
    43801 aatcattttg acgtagaaca ggctattttt gatccagatt ctgaaatagg gaaatgtgtt
    43861 gaattatcag agaaaactgt tagtacaaaa ggggcgatga tcattgagag agaaatgtta
    43921 gacgaaatca gagtaatcat tcctaaagat acgcagatgt gtgaggacag cataagtgac
    43981 gatgaaccaa aatcagaaag aaaataagga gatattcaga tgagcgaaaa atccattgaa
    44041 ttaaaggaat ataagcgaag cgatggagaa aaatattatc ctgtagccag tttttctaaa
    44101 tcagtatatc tggttggacg tgcattggta gttggtgcaa ttggcatata tattttgcat
    44161 tgggtaacaa aggttgtaca actgcttgta gataccttag taagtattct tgtctcgtta
    44221 aatgatgtta acggaacaga tgatttagga ttaaaagaaa tgtttaaaga gttactcaat
    44281 gcgttttttc ccaattattt aagttattca tataatgtac aaagcttttt tgcaaagcta
    44341 cacttggtag acatctctat tttagtatta gaagcaggaa cggtattctt tgttggatat
    44401 ttggttactt tgatgttcag gcatcactat ggagaacaaa gaccattttt tgatgatatg
    44461 gaaagtagaa ggttgaaaag agaagctcta gaagctatgg atgtgaagta tgatccaaac
    44521 aagattatag gagatgaaac gaacaacaaa aaaagaaaac gaaacaaaac gcaattagaa
    44581 tccgatgtgc tatcaacgct taaaaatatc aaaatttctg ttcatacaag acgtgatcta
    44641 agtgtcaatg aagatattcg agaatatatc gtaaaaatcc cgcaaccaaa agggaagaaa
    44701 gttagagaaa cagttttacg acaaattcaa gatttagggt cgatcctgac aagagcaaca
    44761 agcggacgag ccaaatttgg tgaaaaagaa gatgaagttt caggcgatta tttcattttc
    44821 cgagcatcag taaatattga aagagaacgt aaggaaagag taagtaccaa aaacaaagaa
    44881 acggaaagag caacatcttc aatcgcagtt tcaaattcac aattttcttt tccaatcaat
    44941 ctctttgccg ataattccaa aaatatccaa aaccaaaccg aaaaagctag aaaatatgcc
    45001 gaaactcaaa agcaatcttt agaactttat ctatcttctg ctgacgtacc tgcaagtcta
    45061 acagatatgc acgttgggaa tacatctatt gaatatttgt ttactttatc tcgtaccaga
    45121 aaaattcaac taagctcaat gaaagaagaa attgaaactt acttgcatat atcaggtgta
    45181 atcgtgcgga tcaacgcagg gcgtattcaa attactctac cattaggaga aaatgatcgt
    45241 attccaattg atgtaccatc aatgatcaat agcgtgtttt aaaagacatc acaaatagaa
    45301 acagaaaata caaactacca gttcacaaac agaaaagtac cgagaaaagt gaggaataga
    45361 aatggctatg aaattattag agaaagtcaa aaagagagcc gaaaaagctg aatctatttt
    45421 gctagataaa gaacctaata taaagcttaa tggaaaattc agtgtcaata tgttgaacac
    45481 aaaggctaaa gacccgacag atgctatttt tggaattaag cctgatggca caattgctca
    45541 tttttgcatc agcgaaatgc cacacatgtt ggttgcagga acgacaggat cagggaaatc
    45601 agtattactg aatgaaattc ttgttacagc aatgtgtcac tcaacgcctg atgaattaaa
    45661 aataggaatt gtagacccaa aaagggttga atttggaaga tacaaaaaat tgccgttcat
    45721 gttggctgat ccaattacag atatggatga agcttatgat ttttttgaat atcttgtgat
    45781 cttgatgcat gaacgttaca aactgatgga aaaagcaggg ttacaaaaca tcacgctata
    45841 taacgaatat gccgaaaaaa atggattaga tagatttccc tatgtgatct tattagttga
    45901 tgaatattca cagttggttg gaacacataa agaagtagaa ggtttgattg ttcaattagg
    45961 acaaatggca agggcagcag gtattcatgt tatcttagca acacaatctc ctcgatcaac
    46021 agttgtcact ggtattatca aagccaattt tccgtcacgt gtttgcctta tggtagcaag
    46081 tgatttagaa agtaggattg tattagatga aggcggtgga gaaagtctaa gtccaaaggg
    46141 agatatgctt attaagcttg ttgatggaag tatggttaga gcgcagggag cttacatcag
    46201 taacaaagaa attgaaacaa tcttcaatca ccttagaaat actatgccag aaccagaata
    46261 tgttgattac cgagcaatcg tagctgaaac tcgtggtgag ggcggtgaag acgaggattt
    46321 tggtggaatt ggtaaagctc ctgtaagaaa atctgctaaa agaaatttga acacaccttt
    46381 agcaaatcca tcatctaaag agccatcaat tgaaaagaag agtgcgtttg ctattaagct
    46441 tgaaaaaaat aaagaagaaa aagctaatca gggagctaag tcactagaaa aacttcagca
    46501 agaaaaatta gaaaaactag aaaaagcatt atcatcatca acttctgaaa ctcaatcttc
    46561 aaagggtata gataatggac aatcaattac aactaaaaac aagcctaaaa atagtaattc
    46621 aaatctctcg gtaaatgttg ttatcacaac tgataaatct gaaaaaaaac aagtgaacgg
    46681 aactacttca cccatcatgc aaaaaatgat aaaggcaaaa aaacaggtgc agaaatgatt
    46741 atcattagtt cctttttgag atggctaaca ccatataaat atacagatga aatagttgag
    46801 aagcgagcga agaaaaaagt taaaaaaacg atgttaactc ctaaagaaat gctagatcgt
    46861 ggaatattgg gagtgaaggt tttagaagta aaccaaccgt ttgaattgga taatgcgaga
    46921 gataggttgc ttagagctga agttgatgtc gcatactttt atcatgataa aagcaagcat
    46981 caaatagcta tcaaattcaa tccaaaatcg gcatggcgat accaagcaag agatgaccta
    47041 aagcgtagga agctgaaacc gatgctctat cctgaaggaa aagaggcatt tgatagaacc
    47101 catattgttc ccattggatt tcatggtaca gagtcagata accgcctatt ggtagggtgg
    47161 aacagctcac aaaatagagt tcagatcaat aaatttgaga agaaggtaag aaaatttaac
    47221 gatcggaaaa taatactttg gtttgccaat attcagaagc aaccagatgg gacagcaaag
    47281 tgggaagcgg tcatatttga tgaagatggc aaaaaagtaa tgtctgaagt atggcatgat
    47341 aaagctaaat ttgtttggca gtagaggtga aaattgtgtt aactaaccta aaactatata
    47401 gagagggatt taacgaaaaa gtaagtatcg gtgaatctat tgaatattta ggaaagaagt
    47461 acattattat tcgtattctt gaaattaaaa aactttattt tagtaaatat aataatttag
    47521 cagttagagt tctcgtccaa aaggttggaa atccaagtga ctatagaagt gattaccata
    47581 actataaaac agaatcatct gtaattaagc gttataatca atcaaaaaat gatgagagaa
    47641 tattcaaagt aggagaaatt attatttcta agtatgggat agcatacgaa atagtttcta
    47701 ttaaaaaatt ttattatgaa tttgttgatt tggttgtaga atattctgtt cgattgatcg
    47761 ttccgtggag taaagaagaa attaatgagg cattaaaaca tgaacgaaaa agtacattca
    47821 aagttttaga aggtgaaaaa aagtgaaaac agaccttact aggcaagctg agaagtgctt
    47881 atggcatgaa acaaatcgca tgggtgtatt tggttgtttc gaagtcacta tcggttggga
    47941 tggcagtgaa agagtagatt atataactta ttcagtagat aacacagttc gatgttatga
    48001 gatcaaagtt actttagctg atttaaaaag caaggcgaaa cagactttta tcggtgatta
    48061 taactattta gttgttacta gtgatttgtg ggagaaaatg caacaagacg ataaacttat
    48121 gcggaaatat tactatcaag gcgtatacgt gttcaatgaa aaatatccta gcataataag
    48181 tgttaagaaa gcaaggaaac aaaaagtatc gctaggtaca agatcaactg tattagaaag
    48241 catggttcgt tctatgaatc gtgaagttaa gaaactctat aaagtaaaac cattttggga
    48301 gatagcagag gtggaaaaag gatgattcaa acatttgcaa aaatgagtag aagtgcttgg
    48361 attgaaaagc tggaaaatga tttgagtgtt atgcgattag ctccaaagaa attcaaacga
    48421 gagattgcag aaaaagaagc aattttgtcg gtattgaatg cggtagaact ggacgaaccg
    48481 aaaaagtcag ttgatgtacc tgaaaatgta gcagagttac tagattatta tcgtaattcg
    48541 tcagatgtcg atttgttagc tctgatatta acatttcacg attggtatta ctgtcaaaat
    48601 gaagatactc agcatgataa acatgataaa gcagttgact ggttaataga gaatccagaa
    48661 aagttcatgc gtgcttggct agatggctat gatagcaaga aaaagccact ggaaagtaat
    48721 gaggaaacgt atttaacggc agaacaatat gactatgcta gaaaaacagg agattgggaa
    48781 tctggtaaga ctttcaatca cagaaaagat caaccaaaaa ttagtttttc agatatagcc
    48841 gcaattatcg aaggtatcta ctatggacaa gccgatgaag agatcttttc tatacaagat
    48901 ttattagaat ttttgaacaa aaataaggat agctttgtag aactaactga ttatgaggag
    48961 gatacagcaa atggaaaatt atgatccaaa tatgcgatgg ggtacacata cgcttaaagt
    49021 ttcttttcaa cgttgggatt acaagggatt tgtgactttt agaaaagctg gaaattgtaa
    49081 aggattagat gtcttagcgt tggatgaaga ctatctatat gaccatccat tgacggataa
    49141 tccaatcgga tttggattgc ttcctgaaga tgatgagggc aatgaatggt tcaaaatgat
    49201 tcttacaaac gataaaggtg atcaattatt tgtagaagat acttggtcat atttgagtga
    49261 atatattgtt agcattaaaa tcattgattt tgtagcagat aaagaaaaag agattggaga
    49321 aggtaaatct aattactagc taatgaatag caactttgga ggataagtca aaagaataaa
    49381 aaaagaatac tgtgtgtcat tgcagggatg tatctttgca tgatagtatc tgcggttacc
    49441 atttattggt taggaaatca gcaaaacagt ttaattgttg atctggggga gttaaaggtt
    49501 ggattacgaa tgactagatg gcttttatta gctcaaacag ttggtttagc catattgact
    49561 tggatatgtg tggaaaaaat aacatctaat aataatcaag agattgttga atcaacaaac
    49621 ctaagaataa ctgaacaagc aattgaaaag caaaaagaca aagaaaaaaa ggaaataatg
    49681 tcaatttatt tatctagtgg agcatcattt tttcatgaaa tagattcgat gaaactgagt
    49741 agtcaagaaa tcattgatag atggaaaaac atgcctctag gtggtgagtt aatcgttgaa
    49801 gatagtgatg gttggtcagc ttataagaag gacgagattg tagctatcac gattttgttt
    49861 gaatagtaat atattaccac tagataatct gaatatgttg atctgctagt gacgtaccaa
    49921 atgcgtttaa tagaaccttg gtcacaggca gaaattgata aagcagtaaa attgaaccga
    49981 ttatcaaaat ttaacgtatt aggaaatgcc ttttaaaaat cgcaatacac aaacagaaaa
    50041 ataccaaaaa attagcaatg aacgtaggta agaacatgac aataaataat aataacccta
    50101 acaaatataa tgctcttgtc caatgctata tcgaaaacaa aattacaatg caagaagtcg
    50161 ctgatgaatt aggcaaatca aaaagcctag tatcacacga ctttaagcac aatgtttttc
    50221 cgaacccgat tgagctgagg aaaaaatttc aaaagagagt ttatcaagaa atagggaagt
    50281 ggattgatga agggaatgca attgaccctt taattgaaaa atatcctttt ttaaaaagca
    50341 aaaataaaat tcaaaggtta attcgtcaag gcgatattca agctaaagaa cctgttttga
    50401 caaccaatga tttggctaaa ataaatcttc ataaacagat tattgaagac tttgaaaaaa
    50461 atctttcaac aactgaaatt ttaaaaaaat acaatattcc gagaggaaag ctaacatcga
    50521 ctctttttag agaatatgga gaatcggcag ttactaaagc aaaggaagta gcgaatgaga
    50581 gaaaaaaaca aaaatagtag tcgatactta gtagcgactg tagtttttgt ttgtgtattg
    50641 attttcctgt ttgatttttt acagattgga aaaagtataa ttggaaatac attgagtgca
    50701 aaggaaatgg ttgctttagg tggtttgtat gaaggacatt tgttcccgaa tatattcatt
    50761 tctatcgttt ctcatattaa cttgtaccat ttaatttcta atttattgtt cttagtagtt
    50821 cttgaagaag cactgaatga tcagtatgag tggttaacat tattgatcgt tttaatcata
    50881 tcaggatttc taggaagctt agttcctttt ctgctacaaa gcaacatcgt aagtatgggg
    50941 cttagtggag gtatttttgg atggattgct ttatttgcga ttgcatacat ccattttgat
    51001 agcaaggaac atttattact aagtgttttt gtattattga taatcggcat tttattggta
    51061 tctacttttg aatcaggcga ggcaaatgtc ttgtcacatg taatcggatt tgtctgtgga
    51121 atgatcttgg gatttattct aaagaacgtt tcattgatca aacaaaagaa ttaaaaggga
    51181 gtgctaaaaa tgccaaaaag aaaagaaaag aaaattccag tgtttttgtc tgtctcagaa
    51241 atgttatcac ttcaaatact gttaagaaaa aagattgaaa caatagacga agaagttaga
    51301 aataaaaggg ctatgtacga acaacgtgaa gcgctaagag aacgaatcca ctattttgat
    51361 gatatacaca ctaaaatcta tgaagcacgt acaaatcacg aagacgaaag taaattagga
    51421 ttagctcaac aacggatact tgaatggctt aatacgaata ctgatcaaga accgttaaga
    51481 gtgattagta atctttttga atttggaacg aagcattggt ttaatgctgt agagaaggat
    51541 gctgacttac aagaagcgtt tgatacgcta agtaaggata gcaaatccat gcgagattta
    51601 ttgattactt atctaaatga gcatgagttt gaacaaacga aaaccaaaaa gaaaaataaa
    51661 gaatagaaag ttgtgatgaa tcatgctatt aaccctgtta ggttatttat gtttaattct
    51721 tgcttttttt ggggcaatcg ctataattat tggtcaattt caaacaaata atccgattat
    51781 gcgtaacata ggcattggaa ttgtgattgt aacctttatt gtgtttaatg ccactaagat
    51841 ttttggaagt aacgataaac aatttggtat atcgggagtt aataatagtt atgctccaga
    51901 ttctttggga agggtagtaa tagatggaac atttgaaaat gtaacgagtt ataaaaatgt
    51961 ttcgctgaca gtgaaggaag atggcaagaa agcatcagac gacgttctcg ctactattaa
    52021 gccgtctcaa tcacaatttg aagtgtttta tacagttaaa gagaatagcc ctgtacacaa
    52081 gctgacattg gtttttaacg ttgatggaga agaatactat aaagatgtaa aaataaataa
    52141 aacatcaacg aaaaagatta tcgaatcagc gaaaaaagaa gtagctacag aagaaagtac
    52201 aactgaatca agttctactg tcgaagcgga actaccagat aaaattgatt ttgatcctga
    52261 aaaagtcaaa tcgtttgaat ctgtttctgg ttctatggct gatcggttta acactgaagt
    52321 cttcaaagct atcaaaactg ttaaattgga tactgaaaag caaatgatta atgttacctt
    52381 attcaattca gttgaatcta tggatcagga taaaaaagtt acggttgcaa gagaactagg
    52441 aaaaagagta attggtttgt atcgtggtac tgctaaacca gattcagacg gtgcagattt
    52501 gccagttcat gtagtttatg aagacggtaa agtttttgga gattctacag caaacgaagc
    52561 attaacgatt aaattggcta aatagccaga aagttataat aaacaaatgg tgtcacacca
    52621 ataatatata ggtgtgacac cttggagatg atagctgatg gtagtaaata aagaaaaagt
    52681 tagaaagtat gtgactttaa aaatagcttc tgttaaatat ttagaaaagc gagctaaaga
    52741 agaagaaaga gcaagaggta agaaagtaac agtttcagag ttgttagaag aattgatcga
    52801 gaatgatcaa acaataagac gtgcatttac taataggtct atcagataac aatataatta
    52861 ataaaaaaga aagagtgatt tggtcttaga ctaaatcact ctttttatat aaagtttctt
    52921 tacactcatt ccgaataatg ttaaaattct ttatatgata tatagttcct tgtcagagga
    52981 agagataacc agcaatattg gagggataat aagtggaaaa caataatgat attagacatt
    53041 ttagcattgg ggaaagagtt acaacagtaa gtgaattatc aaaggattat agagataaag
    53101 agggaacgat tatttctctt aataagaatc gaagaggcgt ttggcaatat gttgttgatt
    53161 taggtgatgg cgttacagtt gagtgtaaag atagtcaact gatcagaaat aatggcagtc
    53221 attccattga aaaagcaatt cttgtatacc caaaagtatc aagtaagatc atgaatgact
    53281 ggttgcttgg aaaatttggt gagtacaata aaaaagaaat cgcagaagtg atcggcaaat
    53341 cttatccaac agtattaggg tatctaaaaa agataacgag taaagaagca aaaattgagg
    53401 atgttttaac agtaagagag cttcaatcat taatcgagta tcggaataaa caaataacaa
    53461 atcctgagta ccttagaaaa gaagatggtc tattgaaaca attggaacaa gtaaataaaa
    53521 tccggaagat gcaaaaggta gctcctttaa gccttgatac ctttaaaaaa atctatttga
    53581 aagagatcaa aaaagactaa tgtggactgt aactttaccg ttccaacctg tatctgcacc
    53641 aagaccaagt gcaagaagga atgctgctga tgaaactaat gacgtagcag agaaaaggat
    53701 ttcaacctac tatgataaaa agtatcttga ttatttgaaa aaaataaaga gctttatcga
    53761 agatcaagat ttattagatg atgaatttta ctcgattgta aatgatccta tgggcgcaat
    53821 tatggaagtg gacttttact accagatacc aaaaagctat aaaaaagtct ataacattat
    53881 gaagacaaca gcgccagatt tagataattt agtaaaaagt gctatggatg gtatttttaa
    53941 catctcagct atacgagata gccatatcac aggcttaatt gcttttaaat ataatgtggc
    54001 gaacgaagga aaaacagttg tgaggatcaa acgatattca gaatttgagt gggatacttc
    54061 tgaaggattg aacggagata tatgggaagc tacatttgat tttaaaccag tagcaacgcc
    54121 aagaccaaag tctaaattta gaggaaatgg gattatcact tactatccaa aagagtacaa
    54181 cgactatttg gaaaatatca agaatacgct taaagagaaa gatttggtaa atgatcaact
    54241 gaaaaaggta atgaatgctc ctatgggggt tatagctcag atagattatt tttaccaagt
    54301 gagaaaagat aagaaaaagc ttgattcact gatgcgtact tcacagccgg atattgataa
    54361 tttagtcaaa ggaacattgg acggaatttt taacaaggaa ataggcatca aagatagcag
    54421 ggttgtaggg ttaattgctt ttaaatacaa tacttttgaa aagtcgaaaa caaacgtgag
    54481 actacaggag atgggctgat gaataataat gaaaaaattc ttcatgtact tgatagcttt
    54541 gaaataattc aagaagaact aaaaaaatat cgggatgttt tggaacaaag atatgatttt
    54601 gtcaatcagc aaaagagtaa tcacatggat ttcattctca atatgaatga cttgaagaag
    54661 aaattagttg aacgaaaaga acaagaaaag ttgatcaaag cttactttga actcggtgaa
    54721 aaagaagtta aaaatgcaat ggaactgaat gaagagcgca gggttttaga tcagctgtta
    54781 gaacaattat tggtgatgtt tcaaaaagga cgaatagatg aggatttaat tgaagaagga
    54841 ttgagaaaat atccttcaaa ctcaggtatc ggaatagtgc tgaaagcgat tgatgaagaa
    54901 gaaattgaag ctttcatacc gccagaggat tttgaatcgg caatggaata tataaaatat
    54961 tatagccaag ggatcactgc ttttagagag tttgatcctg aagatgttat tcatgacttg
    55021 aacaacctaa aagaatggtg tgaaagttat ggagtcgatg attccggatt ggactacttg
    55081 atcagtatta tggaaatcga agaagagatg ccggataagc ctgatccaac agatatttta
    55141 gaattgatcc atgaagctag aaatccaatt gcctatattt ctagaggata tactgtattg
    55201 gaatactata agccttatat atcagcgatg aaccatttaa gacgtgtatt aagggaaaaa
    55261 cgtgaatacc gctcagtgtt gaatgcaacg aatagattag aaaaagctgt ttcagagcta
    55321 gatgcttatt atcgtgaaca ttacctgcaa gcaggtggta tgccaagaaa tacgaaagca
    55381 aatatcagta ggtatataaa aaaagctgag taggcggagt tagcccataa aatggcaaaa
    55441 ggtaacctga aaggggcatt ttgaatgaaa agggatgttt ttgaattgta cgtattagaa
    55501 cgtctagaag acggcatgat ggcacaagag ataattgatg aaattgatga tatgatcagc
    55561 agatttggtc aattggagtt gaatgatgga aacaaaaatt cggtagtgct aaaacaattt
    55621 tggttgaaca atttttcgta taaggatttt atcaaagaaa aggcaaatac attgtactga
    55681 ctaaattcac gaggagcagt gatgagagct tattgctaag agcagttagg ctcttttttg
    55741 tttgtgtaaa attatttgtt ttataataca catttataca caaaaaatat tgacagatag
    55801 tacacaaatg tggtaggata tatttgtaag caagttagat taacaagtta aaggaagagc
    55861 agttatgaaa aaagaagaac taatagatat atttcaaatt gttgaacgag caaataatat
    55921 gggaattatg ttttttgatc gtatttcttt aaagatggat ttaagcgttg cccaccaaga
    55981 atttaatctg cgattaaaag ccttgttgaa ttctgatgat gttaatttcg ctcatgatgt
    56041 tgtaggtata cagaaccata tagatagaga aaacaaacgt atgggtgatg aattcttacc
    56101 aagatattca agtttatgat ttggcgaaag attcaagagt atgtgtcatt attgataggt
    56161 actcttgaat tggaaaggag gaatgaattt aagacaacaa cgattatgat cttaaaacaa
    56221 tcgtttatgt aattggcaat cttacgaata attttcatgc aataatgaca ataaaaaatg
    56281 aaaaggggta aaatcaaatg aaaaaatttt tagtaggaat tctattcaca atggggctag
    56341 tattattatc accttcagtt tcagcagaaa gctctatttc catgtatcgt atgtataatc
    56401 ccaatagcgg agagcatcta tacactagaa gcgttggaga aagagacaat ctgaaacgtt
    56461 atggatggca ttacgaaggt gtcgcatggc aagctccaac aagtggtact cctgtttatc
    56521 gtttgtataa tcggtataac ggtgaacatt tttacacagt tgatgccgga gaaagagata
    56581 agatttcaca cacaggatgg acgtacgaag gtgttgcgtt ctattcctac aacggagcaa
    56641 atagagttcc tgtaacacgt ttatataaca aatacgtgaa ttggcatcac tacacgttag
    56701 atcaaaatga aaagaacgta attagtaaac aaggttggaa gattgaaggt acaggctggt
    56761 atgcaatgcc aaacagcgta ccaaatccta cacctgctcc aagcactgaa aaagtgttga
    56821 acgttcctgt tgtatggcaa ggaaatacaa tgttatgcga aggagcttct ttattagaag
    56881 gtttacacta caaaggtgta gccacaaatc aaagcttaac cagttttgta aactatatgc
    56941 caaaagctaa tgacaataat ccttatcatg gttactcagg agagtggcgt cataacgtta
    57001 atggaacgta tcaaggcatg atggctgacc cagtagttaa atgggcaaaa aacgttggcg
    57061 gtaatgccgt caatatcact ggttcaggaa cgaatggaat caagaatgaa attcgcaatg
    57121 gtaatccggt tgtaacttgg attacttata actatgctac gccagagttc aaacaaatgc
    57181 cttggggtcg tgcagtatgg aatggacatg tagttctcgt ggatggcttc agaaatggtg
    57241 cataccatgt tgttgaccct gtatttggcg caaaatggat caatgcaagc acgtatgaga
    57301 gagcatttaa tgtgactgga atggcggttg ctgtacgtta attgatagaa ttatcaacta
    57361 aatgaaataa gagcttaatt actgaaatgg taaataggct cttatttttt tagaggaaat
    57421 ttttgtaata cgaatctata acaaaaaata ttgaatgtag tacacaaaag tggtatatta
    57481 aaatcgtaga gattatgcga agaggtgatt ttgatgaacc aaagaagaag aaaaaggtta
    57541 gaagaaataa gcgagcgact ttccgagcta aaggatgagt tagaagtaat tcaagaagaa
    57601 gagtacaaag aaaatatacc taaaaatcta cagggatcag aaagatatga aaaagcagaa
    57661 gaatccgttg atcaacttga agaagctgtt gatggtttat ccgacatatt agaattgatt
    57721 gaagatatag ctagctagag atgattaatg aagatttcag gagtgagaat aaaatgattg
    57781 atatttcact gatagagaag ttacttttct caaataagta caccgcaaaa gagctggcta
    57841 caagatggca aatatccgaa tcagctatta aagcctatcg tcaaaataag ggaagcgtag
    57901 gatggcgtga ttggaaaggg atcagcattg gaaaagcaga agacataatg aaacgtgaag
    57961 gacttcgatc ctataccact tctgaaaaag acaaataact taatgtttga aaagataaaa
    58021 tatctgtctt aaagctatgt caaattcaaa tggtgaatgt ttgctaaata taatgcaaaa
    58081 aaaggaaggg aaatattaaa tgataagcga aaatttgtct tatgagaata ataaacatct
    58141 agttaaagaa aataagaaag tagaagatta taagaacatg tatatagaaa tgaaaaaatc
    58201 accactgtgt attcaatata aaaatgataa ttggaagcaa gtagtcgaat ttgtaaaacc
    58261 tcatagtata cgaagagtac gaagggggaa gaaaaaaagt gaacttgtaa atagcggaag
    58321 agcggtaatt agagttataa ccaaagatta tccagttgaa tatggagatt atattttaag
    58381 gttaagttat catgcttttg ttgtgttgaa agaagagcat ttcaacgagt ttttcaattt
    58441 aaaaagtaag taatcatatt tttttgtaga cgtgtataaa gttaactaaa ttgcgaggtg
    58501 atgacatggt agcaaaaaag aatactctaa aagaagcttc tgaaaaatat caagatatga
    58561 aaagacgagg attaataccg aaagacaaaa aaggtatatg gagagaattt gttaaggaag
    58621 tcagtagtga tcgaagtggc ggtgactttg ttggacgata tacagcaaaa ggctatagca
    58681 aacgattaag gatcactgtt gaatatggaa cgaatgaatc agctacagag ctgaagcaaa
    58741 ggtacttaga tacacgaagg gaagcttcaa aaaaatataa tgatttcaag agcagaggtc
    58801 tgattcctaa aaagaaaaag ggaatttggc gtgagttttt caaggaagaa gatagcaatc
    58861 ggaataaggg agacttcatt ggacgataca cagcaaaagg ctacactaag aatttaggga
    58921 tcaaggttga atacggaact ggtgaaagtt catgggaatt gagaaatcgc tatttaaata
    58981 cattgttaga gaaatataaa gcagaattat tggagttgtt cgatgttaat gattttatcg
    59041 agatggctga aaagtatggt catgaagaag gttttatgat caatgacatt atggttattg
    59101 ataattttac ggatttatta gcacttgcga aaaaagcaag ggatttaaga gaaacgcaga
    59161 tgtatctatt cagaaataaa aacggaagaa accggagaac tgaatcgagt gagattgaag
    59221 attactacga tgcctttaca cacgatctgg gagcctttgt agacgagata agtggtgtaa
    59281 tgaacaaata tagggtctag aataaaaaag ccggagatga gcagataaat gggaaacaat
    59341 agcatataca cacctgaaat gcagaataaa attaaaagac ttgtcggatt agggtggatg
    59401 acaagaggaa aattagattc ttttagtacg atcaaatatc ttttgatgga catttaccgt
    59461 cagaacaagc aaacaggaat acattttttg ggctatgata agccggaaga tgcacctttc
    59521 tttgttccaa gttcagccta tagagccagt acctccggta gcatgtataa cttggatttg
    59581 tttgaggaag gagcaaagat gatcgaatat gatttcaacg aagcctattc gaggatcatg
    59641 agaaattatc ctttgccgtc aaacacttat ttgaaagcaa aatataatac agatcaagct
    59701 attttgagag cgataaatag accaaaagta agaccgtatg aaaatttaag aacgtatatg
    59761 ttctttttgg ttaattttga aggaacagcc aaagaaggaa catatgctaa tccaaaatca
    59821 cagtcgatgt ttgcggatta tggcgaaaat cccaaaacag ctaatgagcc tatttggtta
    59881 tcggagattg agctaacact tatccttgat tattatgata ttaaagagat ggaagtccta
    59941 gatagccaca catttttgac gaaaaaagga ttactcaaag aatattttga aagaatcgag
    60001 ccattaaaaa ctgatcctga tacgttgaag ctttataaaa atcttagaac gaaagtattt
    60061 ggtgctatcg gacaaaaatc attaagagaa ggagaaacaa gcaccttcga taaatacagt
    60121 atgtataatc gagcgtattc ctccatggta gcaggcgtat ttagagatcg tatggttaga
    60181 tatgaacaga aatacgttca tagcgcatat gggctattaa aaattcgtac tgatggattg
    60241 tatttcagat ccgaagtacc agagtttgaa gagctgtata aacttggagt agttaagaaa
    60301 aaagttcata caatatccaa agaagaagtc gaaaaagctt tagaatttaa aaacaaagga
    60361 tttgaaaata agggctcctt tgggaaatct aaagatgaag ttaagggtgt tgtgatagac
    60421 gatgatttcg agtttgattt tgactaaata ctctttggaa aagatggtag gaggtaaata
    60481 tgaagaagaa aattgtggaa ggaaatgtag gtgttattat tctaaaagat tcaactgact
    60541 atgctaaatc aatggcacta aaagacgaaa aagttgcacg catggataaa gatttagttg
    60601 aatttctgat ggatgataca gaaagattaa aaggggatca gtttttgagt ttaatgaaag
    60661 acaaaggctt aaacacatat ggtttatttg attataagaa attagctata gagtgggtaa
    60721 aagaaggaga aatatttttt attgaagaat gggaaggtac tgagaactta gttcttcatc
    60781 agtatctttt gaaagcttaa tctaagtaaa acagttctga ctagttatgc ttgtcagaat
    60841 ttcaaaggaa gttttccata accatcatca ttcaaaaata aaggatttga aaataagggt
    60901 tcctttggga aatctaaaga tgaagttaag ggtgttgtga tagacgatga tttcaagttt
    60961 gattttgact agattttttt gaaaggttta agagtaaaca aggtaagaac taagatcatg
    61021 aagattgaga gtaaagaaga tagaggacga atgtatgaga acacactata tttatttaga
    61081 agataaaatt gtagatgaaa ttccggcaaa aagtcgagaa caagctagaa aattatttat
    61141 tgaaaaatac aaagagatca atggagatag tgaagttcca aaagtggtcg taacagttag
    61201 aaaaaggcaa acaaaaaaga gaagtacgtt tcatgtcagc cctgatatat ataatcttat
    61261 agagcgacat agaagtgaag ataatatttc tatctctcaa tatattgcta cagttattga
    61321 tgaattgatt tcaggaaagt atgactatgc ttttgatttt gagggtgaaa aggattacaa
    61381 aagtgttgct gtctttcaat ctgattttga gaagctaaaa aactatgctg gcaggagaaa
    61441 tgttaatttt gcaagactac tagaagagtt ctactccgaa gagttgggag taggaaatga
    61501 atacaagaga gaaacttatg cgaaattgtc tcataagtta tcccaagcgc ttaaagaagt
    61561 gacagaagtt aaagcggaac tagatgctaa acttgaagaa gtgaaaaaat atcaaacaga
    61621 gaggaagaac aacatgaaga atcaagtgga atttgacgaa cagaaagaat ttgaaaaagc
    61681 aagaaaacgc tataacaaaa ttatgaatga acttagctat gaacacttaa cagttcatga
    61741 ataattgagt gagggtacag aaaaatatac gatcgcagat atggttaatg aagtggaaac
    61801 tcttttagct tctttctttg aaaatggtca cgtaaacgat gaatggcgaa aagaagaacc
    61861 taaaagggtt aacgcttata ttgctcgatt aaaaaggttt attgataaat ataaggagaa
    61921 tagtgacaaa ttttctaaag ataaataagg agagagtgtg attacatttg ccagaaatcg
    61981 aaggaatatt ttcgccaaaa ataggtaaaa aacagttaga tttcagcagt tttccgagcg
    62041 taaatagaga ttataatcta ggtgatgagg tacagatact aaatattgat ttcaaagaac
    62101 tagacttcgc tgttgctgtt tccaacaaac ccaaggcatt ggaacgagtg aaaaaaatat
    62161 accaagagaa tgatcaaaat atcaaaaata tgatcaaaaa gtatagacga gataattcaa
    62221 aagttgaaga cgttttattc aacggtacgg caaaacatga tttgaatgtt tatatggcat
    62281 taggagtggc tttgtactgt aattcaatta atcagacaga tattttggtt aatatcgtga
    62341 ggaatagcta tcaaaaaata ttaaaaataa gtagcgaaac aaacccttat tatgcaatga
    62401 aatatcttgg ggataattta ttaaatgata aaaaattgat gcacaacact gaagagctgt
    62461 tatctaattt ggctgcgatt aactttctat tcaaaacagg ttctgaagat tattttatag
    62521 ataacgtact aaaaggtgct tacggaacag cattttatcc gctttacaat atgaaacagc
    62581 ataaagccga acaaaaggat aacaaacttg aaattaaaaa gatgaaagaa ctattaggta
    62641 taggatttgt aaaacccaat tcaaaagtca ctattgcttc gttagcaact actaacaatg
    62701 atgggaaaat agtaaatggg tttgacgatt cagatataga tatattgtca aaatcatatt
    62761 tttctttgta tggagatttt gcagatttga tgggtattga tagttactta gtcaataatt
    62821 ttgagatcaa gggtagcgag ctgaatgata ttgtgtattt cgttttaatc tctctatatg
    62881 caattgatca gaataccgag taccgacaaa ttgactattt gctagttttt tatagtttgt
    62941 tatatcagtt cgttttaact cggttgttta aaaatgctaa ggaagaattg atcctgaatt
    63001 ctgattatga aatagcaaaa caaattgcaa gtattagaaa agaaaacaaa tctgaagtaa
    63061 tcaaattaag aaaagaatta agtattgcga gtaaagaaaa cggtcggtta gaatctttag
    63121 tcaaatcggc aaaaaaagaa gaggaacttt tgaagcaaga gattaaagaa ttaaaaggaa
    63181 tcagaaaaga acttgaacag ctgaggtttg aaaataaagt attaaagcaa ttgcaggagc
    63241 aagaacatag acaatcatta aatggtaaaa ctgaagagcc ttcactgtct gaaatgatag
    63301 cttatctaaa acataaaaac attcttttag tgggaggaca tgtaaatttt caaaataaat
    63361 taactgaact attaccaaat attaaaatgt ttgatgtaag cgaaaataat ggaaagatac
    63421 ttaaaaattt gaatacgtgt gatgcgattt ttctatatac agattacttg aatcatggtc
    63481 tatattatcg tgttatgagt gaaatttcta ataaagataa gatattttat ttggataatc
    63541 atgtgaatat gaacttaaca attaaacgta tttacgatct tttatcactt atataaagag
    63601 agaagtgata aataggacac aaaatttgga aagtaggtaa gtcactatga gagaactaac
    63661 tttatcttct aaggaattag aaaagaaaac tgctttatta aacgaaatat ttagtgctga
    63721 ttgtataatc caaagtttaa aagaaagttc attttcaaaa ctaacagatc aggaaaaaat
    63781 ttcttatatg ctaagtttag ataatctagg aactaaatat gatgaatcag caagtagcga
    63841 taagcttttg attgaattag tatcaagtct tagtagtttt ggaaaaatga aaagatttaa
    63901 tgtgatatta gaaatttttg cttggttaat cccttcattt atttggtttt tttatatttt
    63961 catagttgaa tctgagttaa tagccaaggt ctctcttgga ctgttaattc cgatataaat
    64021 aggttcagca atttacttta ataacttgta tacacctatt caaataaaaa agcagttatt
    64081 acatgaagga ttaagtggta atatgttaaa aagaattgca tgggataaag ataataaaac
    64141 agatattgag cttaaagaat ataaatacaa ttatttctcg agatgtatag atagaaagtt
    64201 actgtttaaa aaagaaggat tagaatttag ttttgatgaa gatgaatttg aaaaattagt
    64261 gatggactgg cgtaagaatt tactggatac ggtacaattc taaaagcaat tgaggtcgaa
    64321 attccgatac ttgaatcatg atctatatta tcgtgttatg agtgaaattt ctaataaaga
    64381 taagatattt catttggaca gtcatgtgaa tatgagctta acaattaaac gtatttatga
    64441 tcttttatat gtttataaac attaatgagg tgctaaaatt gacaacaata actatgacaa
    64501 aaaaaataga agtatatgtt gatggatcac aagaagaaag ggattccttt tttgattttt
    64561 tttggaaaaa tagtagagaa tatgtaaaag cgttgaatct ggtatataaa cagctaatgt
    64621 ttgaagagtt gataaaagat gcaattaaag aaatggatca aggttatgct gagagagaag
    64681 aaagtattca aaatcggatt gaaaagttag catatactga tccaatagat caaaaaaaac
    64741 ttgataaagc aaaagctgaa ttatctaaat tgagaaatga aaaaaataaa gaagcgagag
    64801 aaatattaac gcaagcgata gggttgaaga gtcaaacgag aacacgagat gtagcaaaaa
    64861 aattagactt tgactttgga gattttgttg attccgcaaa tatgaaagct agtaaagatt
    64921 ttggcaatga ttttatcgga attgtgaccg gacaaagaac gcctagaata tataaaaata
    64981 gaaacaaata tggattatta ccttttagag gtcgagatat taaaatccat aaagatgata
    65041 agttttatat ttatttacct aaaggcttta ggtttaaagt gaatttaggt agcaagccaa
    65101 gaaaagcaag agatgttgct tcagttttac aacacattat ggatggcgat tacaaattaa
    65161 atcaaagtaa tcttattttt gatggaagaa aattattttt taatctaaca atttcttttg
    65221 aaaaaagcta taatgaaatg gaattcaaga aaggtaatga attgatcgta aactttgcag
    65281 atttgtgtag cggtgaatgt ttaattaatg ataaatctgt aatgtctttt ggtgatagcc
    65341 aatatataag aaacttcaaa agtaaaattt tagcaatgaa atcaaaagaa caaagccgag
    65401 gagtcttttc taagggcggt catggtcgaa aaagaaaatt agatcaagat cgatgggaag
    65461 caataaagaa aagagaaagt aattttgtta aaacttataa taatcaatgt tctgcaaaaa
    65521 tcgttaaaac tgctattcgc aataaagctc aaattattcg tatcattgaa ccagataagg
    65581 cttatggcga atgggcttac tatcaattga aagaacaact agtaaacaaa ggaaaaagat
    65641 atgggataga agtcagaagt tagtaaatta aacaatcgtt gatttggcaa cgaaatagtg
    65701 agggaaaatc cactcactga agggtaaact ccgtcaagtt gtgtgaccag acggaaggac
    65761 gaatgttgtt gaggttttag aaccatataa aacagcatgt ttctcaaatt tttaggataa
    65821 gggaaattgc tagcagtttg ttttagaacc atataaaaca gcatgtttct caaatgagta
    65881 ctttaaatta attgatggcg ttcctgcttt agaaccatat aaaacaacat ggatttaaaa
    65941 caagaagtaa gtcagttctc cttttacttt agtaaggaaa aataaagtat tctcaataaa
    66001 acttaaaata agcaaacctt tgttttaaaa tgattgtcaa gttaaaaaaa tctgggatca
    66061 tataaaatag tatagttctc aaatatgatt agaaacccta ttaaagagca atattaagcc
    66121 aacttactta aatcgtttaa ttaagaacaa taatctcttt tagtcagact tttagtctga
    66181 cttttttgta tttccaaaat ttattcttta caaaaacgaa catacgtttg tataattact
    66241 tgtgaatggg ggaataaaaa tgtttgatta cagtagagaa ccggtgaatg actatttttt
    66301 aatcgacatg aagtctttct atgctagcgt ggaatgtatt gaacgaaatt tagacccaat
    66361 gactacaccg ttaattgtca tgagtagatc ggacaacact ggttcaggtc tgactctagc
    66421 ttcttcacct actgcaaaga aaaaatatca tattactaat gttagtagag ctactgattt
    66481 accttatcca ttaccaaaag aacttcatat tgttccacca cgaatgaact tatatataaa
    66541 aaaaaatatg gaagttaatc agatttttag aaagtatgtc gcagatgaag atttattgat
    66601 ttattcgatt gatgaatcaa ttttaaaagt gacaaaatcc ttaaatttat ttactatata
    66661 aattgagtta aattgtattc gtttttcctc ctataggcgt atactaaaga aaacacttga
    66721 ggagtgggga acatggaaac agaacgccaa atccgtgaat tggaaaaagc gatgaatacg
    66781 acagacaacc gccggttgta cgagcgtttc ctggcagtca agttggttct tgaaggccac
    66841 acccggaaag aagccgggca caccatcggc cgcgacgagc atactgtcag ccactatgtg
    66901 aaaaactact gccataatgg aatccaaggt ttagtctcca aaaaacaaag cggacgacct
    66961 tcacggctga cagatgaaca ggaagcggaa ctggtccaaa tcgtcgccta ccacactccg
    67021 gaggaagtag gtttcaaatc ccgtgccaat tggacattgg cgattgtgtg tgacctgatt
    67081 ctccgggaat ggagttatgg atatacgcaa aaaggcgtgg cggacctcct tcaccggcta
    67141 ggactaagct ggacccgtcc gacgtatacc ctcaaaaaag cggattccaa gaaacaacag
    67201 gtatttttgg aagaaacctt cccgagccta aaaaaaaatt gctgaatagc gatattgacc
    67261 acctcctatt cgaggacgaa accatgatcc gggattacca agccattggc tgcacctggt
    67321 ttctccgcgg ccaccagcga aaaatcccca cttatggaca acacaaaggg gtaaaattga
    67381 taggcacctt ggattatgaa accggtcgga tcatttgtac agaagaaggg gcatacgatg
    67441 cagcggcctt cctccgtttt ctggagcgga cgttgcggga ataccccacc ggaaaaatcg
    67501 tgatggtatt ggataatgcc cttatccacc acgccaagct gatccagtct tttcttgaag
    67561 aaaacgctag gcgcctggca ctcatcttcc tccctcccta cagccctcag ttgaacctaa
    67621 tggaaggcgt ttggaagtgg ctcaaggaat cggtcatcaa caatgttttt tttgaccacg
    67681 tccagaaaat caaacagtcg gtccgcggtt tcttagccga tgtaaacgag cgtccccttg
    67741 aggtgattga ccgactgtgc gtgcggatgt aaagtattta attcaaccta tatagttcag
    67801 gaacaagaag cgagagaaga agagaattag ctaaaataat caaagatgag attcagaaag
    67861 aaacaggtct aatagcaaca gtcggaatag gagataaccc attattagct aaacttgcac
    67921 tggacaatga agcgaagagt agtaaagaat ttatagctga gtggacttat gagactgtac
    67981 ctgaaaaaat atggaaaata aacgagatga ctgacttttg gggcattgga tcacgcatga
    68041 aaaaaagatt gaatcatatg ggaattatga gtatagaaga tttagcaaat tggaatccct
    68101 acacaattaa aagtcagtta ggtgtaatcg gactccagtt atatttccat accaatggaa
    68161 ttgatcgtac ggatattgct attccaccag aaccaaccaa agaaaagtct tatgggaatt
    68221 cacaagttct accaaaagat tatacgagga gagaagaagt agaactagta gtcaaggaga
    68281 tggcagagca agtagccatt cgaattagac aacacaattg caaaactggt tgtgtacgct
    68341 tagcaattgg tacgtcaatt ttggaagaaa aatcaggttt ctcgcaccag atgaaaatac
    68401 caattacaga taatacgaaa gaattacaaa attattgtct ttttttattc aataagtttt
    68461 atgaggggca ggaagtacga catataggag taacttatag cagattagta tacacagatt
    68521 ccctacaatt agatttattt agtgatccaa gaaaaaagat tgctgaagaa aatttggata
    68581 ggattattga taagattaga caaaaatatg gatttacagc tattatacat gcttcatcta
    68641 tgttagaagg tgcaagaagt attacgagaa gcactttaat aggaggacac gcaggaggat
    68701 taggaggttt gcataatgat tgaatataac gatagaggac gtttaaaatg gtcgggattt
    68761 tatttgtctg atcatactga aagaatcaat gcagacaatg tacaacgtgc taatcataat
    68821 ttggcaaaag aacaaatgac gactgaagag attgcgaaaa tactgagaac tgctgtcata
    68881 aaaaatagac ccgtctgcat tcaaaaagag gaacgaaacg ctgaaggtta ctatccagat
    68941 gatgtagtag gaaaaattat tggatatgac gagcttggtg tatttgtgga agaagaaaaa
    69001 gttcactatg atgaaattag gaatgtttct ttctcggaat tattaaaatg gagtcagata
    69061 tgaacattat taaaaaatac gaattcggtt atattacgta tgaacagcta atagaggaat
    69121 tatggggata tggtcaacaa ttaattaatg aagtggggat tgattgcttt tgtttctata
    69181 ttgaatccgg agcaggatac cataagaaca gatactatat atttccatat ccaccaaaat
    69241 agtctcttag atatttttaa attttctcca aatactttca attattgtga aatatttccc
    69301 tttaattaat caaatttttt ataaaagtaa tatataaaat aaattgcttg aagtattata
    69361 tttagatatc ataatttatg gaagaaaaat taattaaaaa ggatgagatt ataatggggt
    69421 tactggttcc gagagctaat acttatacta tacctttaac gaaacgccaa ttaattgcta
    69481 aaagatatca aaggattact agagccatca atagagagtt ttggaattct gaaagtgata
    69541 ctgctcacag tttatacgta ggttcttatg ggagaggaac ggcaattagt actagtgata
    69601 tagatataat agttgaatta ccgatggcag aatttgacag attcaaaaat tatttatcaa
    69661 atggcccgtc taagttatta caagttataa aaaacgcatt tcaggaaata ctaccaaata
    69721 gtgatattag agctgatgga caagttgtta aaataaattt ccatgatgga atcaaatttg
    69781 aaatagttcc agcttttaat gaaaaagatt attggggtga gtcaaaaggg tttatttatc
    69841 cagattcaaa catgggaggg aactggaaag ctacaaatcc caaaaaagag caagaagcca
    69901 tgaaattaaa gaatacaaaa agtaataatt tattatatgc tacgtgtaaa cattttcgac
    69961 atgtacgaga tacagaattt acgagttatc atctttcagg aatagtaatt gacagtttcg
    70021 tatatgaggc tatgggaaat tggaaattcg ttgaaaataa cagtgggggt caaaacatta
    70081 gttctgtatc ttatgagaca gctttattag aatactataa ctcacataaa gttatgggcg
    70141 gtttaaattt atactctcct ggaagcaatc aatttgttaa ttctgatagt agtatcatat
    70201 gtttagaaaa agtattaaaa aaaatagctt tataaagttt tatggaggaa caacatggga
    70261 gacgaaaaaa agcgcttgga agttcaatta cgtgaagcat atggacgagt aacgtatact
    70321 tacaccactc atttaaagca agccgataat attatttctg acaataaaat tttaaggtgt
    70381 cttcaaattg gtttgtcagc ccttactact ggaggggtac ttagtaccat tattactgat
    70441 aatagtgagt taacctttgt tgctggatta tgttcaacaa tttctcttgg attaaactta
    70501 tactttaaga attttgatct tgctgaagaa gctaaaaatc atcaaattgt tgccgataaa
    70561 ttatggttga tcagagaaga atacgtaagc ttactaacag atttaaatcg tttaagtgtt
    70621 gaagatattg tgaaaaaaag agatcaatta atgaacgaaa ctcatgaagc atataaaaat
    70681 gcccctaaaa caaatagtaa aagctatgaa tcagctcaga aagcattaaa atctgaagaa
    70741 gagcaatttt ttacagaaga ggaattggat aagatgttac cagagcatct tagaaaaaag
    70801 agttctaaat attgaactct ttttttatgc ttaaaattct cttgtaattt ttctattcat
    70861 ctatatttaa gaagaaatat gatgatagaa acatctccta aagccaccgc aagccgttct
    70921 aagcatagct tagcccgaca ttgctagata tgctcatctc accatgaacg tttcctaaag
    70981 ccaccgcaaa ccgtcctagc catcgcctag tccgattttg cttgatcggt tccatttctg
    71041 tttatttagt gggtcaacac cgtaatctta aatagccatc gcctatttaa gattactagg
    71101 tctgctttga aggtaaaaag tctttcattc ttcaagactt ttaggctttc aagctacgcc
    71161 tgaaagcatt gaaattcatc gctctttatt tggttttgcc atttataatt ccttacgccc
    71221 attatctttt tcgcctgtca taccttgtca gaagtgttta gagcttatct catctttgga
    71281 ttaatacaag tggcgtaaat ttttttctaa acgcaagcgt tacgaaaata atttacttaa
    71341 acacttgtat taatccggca gataagaacg cattctaatc actcccgaca aggcaagaca
    71401 ggcgaaaaag ataaataaat gaacgaaagg aagaaacata aaatgcaaaa ccaaatagcg
    71461 atgaagaaaa aaccacctcc ctctaaaaca atccaaacaa aaaagaattt tgaaaaacca
    71521 aaaatcaaaa acagaaagaa gcgttcaaca tgagagatag aattttagaa gttaatcaag
    71581 aaatggtaaa caagttaaca aatatcagtg ggaactatga aaagtacgat ggtactttac
    71641 ttgataacta tatttttcac gatacacaaa atatcagaat tggcagaagt aaaccgagag
    71701 cgttcattct catcaaagag aaatatttga atgaatggaa tagcacctat gaaatggtta
    71761 tgactgataa ctttgaaaag gtattggatt tcagaaatgc tttgttgggt tctgatatgg
    71821 gaaatagcag tcttgaatct attgaagttg aaaacaagac cgagaatgaa ggtgaaggat
    71881 tgattaagaa tgtcttgaaa atcgaacaaa aatatatcag tgaagataga agtttttata
    71941 gagtcacaag ctcggttggt ctagcaaact ttgctactaa cttaattgga aacaatgcta
    72001 gtgaatcttt gctagtgatg tgtctgaata caaagaatga ggtcattgct tatagcgaag
    72061 ttttcaaagg tggaattaat caatcagtag cgataccaag agagattttt caactagctt
    72121 tgctaaacaa ttcagcaaaa atcgcagtat cacataatca tccgagcgga tatgaatttc
    72181 caagtgaaaa tgacaaaagt tttactacga atttgaaaaa cgcaggaaaa acaattggta
    72241 ttgaattgtt agaccatatc atcgtaacaa gtaaaaacac aagctattac tcatttcaag
    72301 aaaatggttc tttataaaaa ttagagaaaa gaggaaaaca agatgggcga aagaacacaa
    72361 gcgttaatta cagtcaaata tggtaacagc aaaacgaaag caagcgtgtc gattcattat
    72421 cagtgggggt atggtcgata tatgctgatg gacgtattga atcaaggaat ttatttaatc
    72481 aataaagcat acgacttacg agataaatca agagaagaat tagcaaaagg aatctttgat
    72541 agaagtacag gagttatcaa ttttgtcacc tatgaatctg gagtgagtgc ttatgatgaa
    72601 acagggtttg gtgggcgcta tgaaccttat caagaaacga ttgaagatgt gatgaagttt
    72661 tcagataaca atgatggcta tgctcattta gagatggtgt tcgatgaaaa tggatttagt
    72721 ctagatgata aaaccagatt gcgattgttt gattttgagg gtcaacagat aggactaaac
    72781 aagttttgtg aaagcggaaa agagtttgct acgaaagatt ttattaaggg atacagatta
    72841 ttgctagaaa attatggaat aaacctagaa gaagagagcg tgaaataagt gtttgaaaaa
    72901 agaaaagacc agttccctaa tttagaagcg aaaagtcaaa aagtgattga tggtgaaatg
    72961 attgaattat tccaaaaaag ttatggaacc atagaaacta ccgcaagttg caaatactac
    73021 tttcaaggaa agagaaacac gtttaaaggc tttgccgatt ctgatgaaga attggcaaag
    73081 aaaatcattg attggcttgt cagcagtgaa cacgtagcaa gagaagatac cgttagaaca
    73141 acgattaatg gggaagtcct aacaggtact agatgtgttt gttgtaatca tgaatttgta
    73201 gaagatgaag gttgctaccg aatcaatgaa tatggtgatt ttatttgcca gagttgccac
    73261 gagatagaat ttccaaccga aagaagttgg tttgaataca tgctacaaga ttatgagatg
    73321 gaaacagaaa atggtgaagt agttttttgt accaaagagg aattgagtaa gagaagcgat
    73381 gatgaaattg aagattggtg tgtgtggtta gtagggacag atgattgcgt tgcatattac
    73441 acaacagcag aataggagtg tgaatcagca tggaaggaat ggattttata gatcatgaag
    73501 atttgattga ttttggttat acatggaaag gaatggtagg tattagcaga agtcttgcta
    73561 atgcctttta cgaaagaaat tatgcagtct atgtccttta tgatgacaat acagaaagcc
    73621 ttgttgatga agagtataaa ttggatttag agaatgtttt atatgggata gaaaaagaag
    73681 atttagcaaa atatattttt tcatggttgg gtcaataaac gaaagagggt agcaacatga
    73741 caaaattcaa aagtaaaggt atgcaatggt gttcttatta ttatgttggg aatgatgaag
    73801 agcttttgag aaaagccaaa caaaaaggtt atccagataa gatgttaaaa cagattggct
    73861 attacatgcc agaagagaga atagaaaaag aatcttgtaa ggttcttttt gatagtgaac
    73921 agctattagt tttgttaact caaaataaat tgatttgtca atttgaaaat aattaatatg
    73981 gggtgaagaa ccatgaatga gaataagatg gtattaagtg aatacgcaag attgagaacc
    74041 attaattggt tgaatggtat gcaaagtagt ggcgcttttg atggatttga tgaggagaat
    74101 atggactttg aaagtttatc gaatgaggat attagatatt attttatcga atggtatttt
    74161 acagaaccag atgaagaggt ggaagatgaa ccagaattaa ttttagaacg tcaaagaatt
    74221 ttattcagtg aacaagatag taagaggttg tatggtttat atgacttaca actgaaagga
    74281 ttaaaagcgc agtatgttgc tgatagttac gatgatatgg tagaacaggg ggcagatagg
    74341 gttgtcactt attcagaaga tgatgaagag gaatgggaaa actattcaga tgatgagata
    74401 tgctttaatg atgggtatat gcctattgaa atcaccgaag aagaatatga taaagtgtcg
    74461 gatagcgatg agattggtat gtgtacggtt gtaagtggat tggatataga tgtccaaaga
    74521 gtagaggttg cataccagaa gatgaaagaa cgtataaaaa gtaaagtaga atcttgattt
    74581 gattggattg tttgcaaagt agctaattaa gagtataata taatcataaa tgtagttaat
    74641 ggatataata atgagtatat tatactcaaa agaggtgaac gaaatgaagg taaaaaatcc
    74701 aaaagaaaaa agcagaattc aagtcggaat agacaaggat ttaaaagaaa acgcagaaat
    74761 gatattggaa gagctagggt taaatccaac aacagcaatc acaatccttt ataagcaagt
    74821 ggtcgctaga ggtgagtttc cagtagagat aaagctaagc gaagaggaaa agcaaggaat
    74881 cagattgcaa cagctaacta tggatatgcc agttgatgtt ctagatacag atgaaaagtt
    74941 agaagagtgg ttcaatgaag cctaatgaga tagtaacggt ctatgttgca tttacagata
    75001 aaaatggtgg taaaagaaga cctattttgg tggttagtga taaagaagat agagtagaat
    75061 tttttggaat cacaagtcaa tatgagaata aatcagacca aataaagaga gtgtattttc
    75121 ctattgcaga atgggaaaaa gtaggtctaa agaagcaatc atggattgac gtagggtctt
    75181 taaaggctat tcccaaaagc cgagagaaca tatcatttaa aaaggttggt atgctatcag
    75241 ctattgatac gaaaaagcta aataggttta tcgagagatt acaaagaaaa gtctaacaag
    75301 gcttttcttt ttttgtttat atgtatatta agatatacgt ttatataaac gtatttatgt
    75361 atattaaaat acacgtaaat atatacgtga ttataaaaat taagaaaata ttaagtattg
    75421 ttaaatagtg ataattagta atatacgaat tataagatac attttatatt taatgtatgt
    75481 aaataaataa aatgactgac ggatattgaa tagtaataaa taacaataat tcagacggat
    75541 acagtgagtg aagtagcttg ctacgaacga acaggcagaa tgaaacggta gtttcattag
    75601 agcaagtcgc ttgcgacgta gcgttaccac tgatgtaact ggcaagttac atagagagag
    75661 tttgcttgca aacgaacgtt acccacttga atagaaaaca aaaataacat attagtgaat
    75721 tttgaatagg agccacaact caaccaaccg cttgcggtgg gtgagtccca cggaggaaaa
    75781 gacgaacgaa cggtcgcgta gc
//
LOCUS       DAZKSU010000004        70849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000004 DAZKSU010000000
VERSION     DAZKSU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.004"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(290..1087)
                     /locus_tag="ACG8Z3_000262"
     CDS             complement(290..1087)
                     /locus_tag="ACG8Z3_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0822613.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="ACG8Z3_000263"
     CDS             complement(1084..2268)
                     /locus_tag="ACG8Z3_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0822614.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="ACG8Z3_000264"
     CDS             complement(2293..3540)
                     /locus_tag="ACG8Z3_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0822615.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="ACG8Z3_000265"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="ACG8Z3_000265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0822616.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="ACG8Z3_000266"
     CDS             5520..5834
                     /locus_tag="ACG8Z3_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0822617.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="ACG8Z3_000267"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="ACG8Z3_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0822618.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /gene="tmk"
                     /locus_tag="ACG8Z3_000268"
     CDS             6519..7163
                     /gene="tmk"
                     /locus_tag="ACG8Z3_000268"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0822619.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="ACG8Z3_000269"
     CDS             7178..7507
                     /locus_tag="ACG8Z3_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0822620.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="ACG8Z3_000270"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="ACG8Z3_000270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0822621.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="ACG8Z3_000271"
     CDS             8495..9319
                     /locus_tag="ACG8Z3_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0822622.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="ACG8Z3_000272"
     CDS             9312..9659
                     /locus_tag="ACG8Z3_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0822623.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="ACG8Z3_000273"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="ACG8Z3_000273"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0822624.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="ACG8Z3_000274"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="ACG8Z3_000274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0822625.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="ACG8Z3_000275"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="ACG8Z3_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0822626.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="ACG8Z3_000276"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="ACG8Z3_000276"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0822627.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15316..16083
                     /locus_tag="ACG8Z3_000277"
     CDS             15316..16083
                     /locus_tag="ACG8Z3_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822628.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="ACG8Z3_000278"
     CDS             16073..18115
                     /locus_tag="ACG8Z3_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822629.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18396..18545
                     /locus_tag="ACG8Z3_000279"
     CDS             18396..18545
                     /locus_tag="ACG8Z3_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0822630.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18556..20070
                     /gene="dltA"
                     /locus_tag="ACG8Z3_000280"
     CDS             18556..20070
                     /gene="dltA"
                     /locus_tag="ACG8Z3_000280"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0822631.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20067..21272
                     /gene="dltB"
                     /locus_tag="ACG8Z3_000281"
     CDS             20067..21272
                     /gene="dltB"
                     /locus_tag="ACG8Z3_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0822632.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21317..21550
                     /gene="dltC"
                     /locus_tag="ACG8Z3_000282"
     CDS             21317..21550
                     /gene="dltC"
                     /locus_tag="ACG8Z3_000282"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0822633.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21553..22821
                     /gene="dltD"
                     /locus_tag="ACG8Z3_000283"
     CDS             21553..22821
                     /gene="dltD"
                     /locus_tag="ACG8Z3_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0822634.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22962..24044
                     /locus_tag="ACG8Z3_000284"
     CDS             22962..24044
                     /locus_tag="ACG8Z3_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0822635.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24044..24847
                     /locus_tag="ACG8Z3_000285"
     CDS             24044..24847
                     /locus_tag="ACG8Z3_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0822636.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24917..25657
                     /locus_tag="ACG8Z3_000286"
     CDS             24917..25657
                     /locus_tag="ACG8Z3_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0822637.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25668..26672
                     /locus_tag="ACG8Z3_000287"
     CDS             25668..26672
                     /locus_tag="ACG8Z3_000287"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0822638.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26717..28561)
                     /locus_tag="ACG8Z3_000288"
     CDS             complement(26717..28561)
                     /locus_tag="ACG8Z3_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822639.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28773..29291
                     /locus_tag="ACG8Z3_000289"
     CDS             28773..29291
                     /locus_tag="ACG8Z3_000289"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0822640.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29419..30102
                     /locus_tag="ACG8Z3_000290"
     CDS             29419..30102
                     /locus_tag="ACG8Z3_000290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0822641.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30411..30484
                     /locus_tag="ACG8Z3_000291"
     tRNA            30411..30484
                     /locus_tag="ACG8Z3_000291"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30445..30447,aa:Arg,seq:tct)
     gene            30643..31002
                     /locus_tag="ACG8Z3_000292"
     CDS             30643..31002
                     /locus_tag="ACG8Z3_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0822642.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31125..32756
                     /locus_tag="ACG8Z3_000293"
     CDS             31125..32756
                     /locus_tag="ACG8Z3_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0822643.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32818..33459)
                     /locus_tag="ACG8Z3_000294"
     CDS             complement(32818..33459)
                     /locus_tag="ACG8Z3_000294"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0822644.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    33645..33721
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            33741..34049
                     /gene="rplU"
                     /locus_tag="ACG8Z3_000295"
     CDS             33741..34049
                     /gene="rplU"
                     /locus_tag="ACG8Z3_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0822645.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34076..34420
                     /locus_tag="ACG8Z3_000296"
     CDS             34076..34420
                     /locus_tag="ACG8Z3_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0822646.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34433..34726
                     /gene="rpmA"
                     /locus_tag="ACG8Z3_000297"
     CDS             34433..34726
                     /gene="rpmA"
                     /locus_tag="ACG8Z3_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0822647.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35160..37967
                     /locus_tag="ACG8Z3_000298"
     CDS             35160..37967
                     /locus_tag="ACG8Z3_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0822648.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            38158..38649
                     /locus_tag="ACG8Z3_000299"
     CDS             38158..38649
                     /locus_tag="ACG8Z3_000299"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0822649.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38752..39741
                     /locus_tag="ACG8Z3_000300"
     CDS             38752..39741
                     /locus_tag="ACG8Z3_000300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0822650.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39774..40577
                     /locus_tag="ACG8Z3_000301"
     CDS             39774..40577
                     /locus_tag="ACG8Z3_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0822651.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40598..41512
                     /locus_tag="ACG8Z3_000302"
     CDS             40598..41512
                     /locus_tag="ACG8Z3_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0822652.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41595..41969
                     /locus_tag="ACG8Z3_000303"
     CDS             41595..41969
                     /locus_tag="ACG8Z3_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0822653.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42108..42353
                     /locus_tag="ACG8Z3_000304"
     CDS             42108..42353
                     /locus_tag="ACG8Z3_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0822654.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42525..43589
                     /locus_tag="ACG8Z3_000305"
     CDS             42525..43589
                     /locus_tag="ACG8Z3_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0822655.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43835..44260
                     /locus_tag="ACG8Z3_000306"
     CDS             43835..44260
                     /locus_tag="ACG8Z3_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0822656.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44253..44702
                     /gene="nusB"
                     /locus_tag="ACG8Z3_000307"
     CDS             44253..44702
                     /gene="nusB"
                     /locus_tag="ACG8Z3_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0822657.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44799..45653
                     /locus_tag="ACG8Z3_000308"
     CDS             44799..45653
                     /locus_tag="ACG8Z3_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0822658.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45654..46991
                     /gene="xseA"
                     /locus_tag="ACG8Z3_000309"
     CDS             45654..46991
                     /gene="xseA"
                     /locus_tag="ACG8Z3_000309"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0822659.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46996..47220
                     /locus_tag="ACG8Z3_000310"
     CDS             46996..47220
                     /locus_tag="ACG8Z3_000310"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0822660.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            47222..48112
                     /locus_tag="ACG8Z3_000311"
     CDS             47222..48112
                     /locus_tag="ACG8Z3_000311"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0822661.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLTRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            48121..48939
                     /locus_tag="ACG8Z3_000312"
     CDS             48121..48939
                     /locus_tag="ACG8Z3_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0822662.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            49046..49495
                     /locus_tag="ACG8Z3_000313"
     CDS             49046..49495
                     /locus_tag="ACG8Z3_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0822663.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49513..51195
                     /gene="recN"
                     /locus_tag="ACG8Z3_000314"
     CDS             49513..51195
                     /gene="recN"
                     /locus_tag="ACG8Z3_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0822664.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(51250..51366)
                     /locus_tag="ACG8Z3_000315"
     CDS             complement(51250..51366)
                     /locus_tag="ACG8Z3_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0822665.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51439..52395)
                     /locus_tag="ACG8Z3_000316"
     CDS             complement(51439..52395)
                     /locus_tag="ACG8Z3_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0822666.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52566..52934
                     /locus_tag="ACG8Z3_000317"
     CDS             52566..52934
                     /locus_tag="ACG8Z3_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0822667.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53104..53535
                     /gene="mraZ"
                     /locus_tag="ACG8Z3_000318"
     CDS             53104..53535
                     /gene="mraZ"
                     /locus_tag="ACG8Z3_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0822668.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53556..54515
                     /gene="rsmH"
                     /locus_tag="ACG8Z3_000319"
     CDS             53556..54515
                     /gene="rsmH"
                     /locus_tag="ACG8Z3_000319"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0822669.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54520..54921
                     /gene="ftsL"
                     /locus_tag="ACG8Z3_000320"
     CDS             54520..54921
                     /gene="ftsL"
                     /locus_tag="ACG8Z3_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0822670.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54922..57114
                     /locus_tag="ACG8Z3_000321"
     CDS             54922..57114
                     /locus_tag="ACG8Z3_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0822671.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGMFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            57140..58102
                     /gene="mraY"
                     /locus_tag="ACG8Z3_000322"
     CDS             57140..58102
                     /gene="mraY"
                     /locus_tag="ACG8Z3_000322"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0822672.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            58116..59477
                     /gene="murD"
                     /locus_tag="ACG8Z3_000323"
     CDS             58116..59477
                     /gene="murD"
                     /locus_tag="ACG8Z3_000323"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0822673.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59503..60591
                     /gene="murG"
                     /locus_tag="ACG8Z3_000324"
     CDS             59503..60591
                     /gene="murG"
                     /locus_tag="ACG8Z3_000324"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0822674.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60606..61826
                     /locus_tag="ACG8Z3_000325"
     CDS             60606..61826
                     /locus_tag="ACG8Z3_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0822675.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61982..63307
                     /gene="ftsA"
                     /locus_tag="ACG8Z3_000326"
     CDS             61982..63307
                     /gene="ftsA"
                     /locus_tag="ACG8Z3_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0822676.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63330..64571
                     /gene="ftsZ"
                     /locus_tag="ACG8Z3_000327"
     CDS             63330..64571
                     /gene="ftsZ"
                     /locus_tag="ACG8Z3_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0822677.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64588..65265
                     /locus_tag="ACG8Z3_000328"
     CDS             64588..65265
                     /locus_tag="ACG8Z3_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0822678.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65279..65878
                     /locus_tag="ACG8Z3_000329"
     CDS             65279..65878
                     /locus_tag="ACG8Z3_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0822679.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65959..66180
                     /locus_tag="ACG8Z3_000330"
     CDS             65959..66180
                     /locus_tag="ACG8Z3_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0822680.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            66229..67011
                     /locus_tag="ACG8Z3_000331"
     CDS             66229..67011
                     /locus_tag="ACG8Z3_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0822681.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            67032..67739
                     /locus_tag="ACG8Z3_000332"
     CDS             67032..67739
                     /locus_tag="ACG8Z3_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0822682.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            68001..70799
                     /gene="ileS"
                     /locus_tag="ACG8Z3_000333"
     CDS             68001..70799
                     /gene="ileS"
                     /locus_tag="ACG8Z3_000333"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0822683.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
BASE COUNT        23137 a        12102 c        15110 g        20500 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    18181 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    18241 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    18301 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    18361 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    18421 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    18481 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    18541 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    18601 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    18661 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    18721 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    18781 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    18841 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    18901 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    18961 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    19021 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    19081 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    19141 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    19201 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    19261 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    19321 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    19381 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    19441 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    19501 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    19561 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    19621 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    19681 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    19741 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    19801 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    19861 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    19921 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    19981 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    20041 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    20101 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    20161 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    20221 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    20281 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    20341 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    20401 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    20461 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    20521 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    20581 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    20641 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    20701 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    20761 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    20821 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    20881 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    20941 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa
    21001 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    21061 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    21121 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    21181 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    21241 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    21301 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    21361 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    21421 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    21481 tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt
    21541 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    21601 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    21661 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    21721 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    21781 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    21841 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    21901 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    21961 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    22021 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    22081 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    22141 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    22201 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    22261 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    22321 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    22381 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    22441 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    22501 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    22561 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    22621 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    22681 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    22741 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    22801 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt
    22861 ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa
    22921 tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac
    22981 agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag
    23041 aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca
    23101 gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg
    23161 acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc
    23221 ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag
    23281 ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa
    23341 gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag
    23401 aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac
    23461 gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg
    23521 tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag
    23581 gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa
    23641 atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac
    23701 caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta
    23761 tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc
    23821 cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa
    23881 gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat
    23941 cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac
    24001 ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc
    24061 tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac
    24121 ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt
    24181 cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt
    24241 acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg
    24301 cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact
    24361 tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc
    24421 catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt
    24481 acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg
    24541 gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc
    24601 atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt
    24661 tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta
    24721 tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt
    24781 gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt
    24841 tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt
    24901 tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa
    24961 aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc
    25021 aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt
    25081 gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg
    25141 gcaacttcat cctaagagaa atccggagac attgctttca atttatggac acacaaaaga
    25201 ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga
    25261 agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt
    25321 agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg
    25381 tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc
    25441 agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc
    25501 tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt
    25561 tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat
    25621 agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac
    25681 caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa
    25741 tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca
    25801 gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg
    25861 ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta
    25921 gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa
    25981 tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc
    26041 tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt
    26101 ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag
    26161 taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata
    26221 tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa
    26281 tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg
    26341 atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga
    26401 attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga
    26461 ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt
    26521 tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata
    26581 aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag
    26641 aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata
    26701 tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc
    26761 gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt
    26821 tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa
    26881 ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt
    26941 cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa
    27001 aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt
    27061 cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt
    27121 aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat
    27181 tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca
    27241 ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt
    27301 ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga
    27361 aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga
    27421 taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga
    27481 tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag
    27541 gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag
    27601 tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg
    27661 caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa
    27721 aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag
    27781 ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa
    27841 taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta
    27901 agagaataag tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa
    27961 aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg
    28021 aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag
    28081 catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg
    28141 gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt
    28201 ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct ttgattagta
    28261 aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat
    28321 ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg
    28381 tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa
    28441 taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc
    28501 tccactttat acgtaaataa aaagattctt ttttattata tatgttatta aaaaaagaca
    28561 taaaggtatt cctttctaaa acttgtaatg ttagggtaaa cgttttttaa actaacgtca
    28621 attatttata ataaaaaatg agataaagat gaggaaatag aattagaaac atataattat
    28681 taaagggaaa catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa
    28741 gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta
    28801 taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc
    28861 gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac
    28921 aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta
    28981 ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa
    29041 acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag
    29101 taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc
    29161 tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa
    29221 caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga
    29281 aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa
    29341 catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt
    29401 gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa
    29461 ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa
    29521 gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa
    29581 tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt
    29641 tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt
    29701 ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa
    29761 gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac
    29821 tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt
    29881 ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg
    29941 ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt
    30001 cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt
    30061 gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc
    30121 tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt
    30181 gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta
    30241 gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga
    30301 ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat
    30361 tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag
    30421 ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt
    30481 gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca
    30541 atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata
    30601 aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga
    30661 attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga
    30721 actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa
    30781 tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta
    30841 ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct
    30901 attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa
    30961 ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa
    31021 tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta
    31081 atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg
    31141 tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt
    31201 tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa
    31261 gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg
    31321 gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga
    31381 tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc
    31441 tatttttact tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac
    31501 ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata
    31561 atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa
    31621 cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga
    31681 tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt
    31741 tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac
    31801 aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac
    31861 aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg
    31921 gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc
    31981 aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg
    32041 cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg
    32101 cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc
    32161 cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca
    32221 aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt
    32281 gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat
    32341 tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa
    32401 tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac
    32461 tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg
    32521 gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg
    32581 atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc
    32641 cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa
    32701 cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca
    32761 atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta
    32821 atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga
    32881 gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga
    32941 ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc
    33001 atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag
    33061 tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc
    33121 aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg
    33181 tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac
    33241 ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc
    33301 gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag
    33361 tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct
    33421 taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt
    33481 tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag
    33541 aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta
    33601 acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc
    33661 tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc
    33721 agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag
    33781 ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg
    33841 tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg
    33901 caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt
    33961 tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa
    34021 aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat
    34081 caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca
    34141 tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat
    34201 cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga
    34261 agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa
    34321 tattgctgag attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa
    34381 ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt
    34441 atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg
    34501 tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg
    34561 atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg
    34621 tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg
    34681 taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaattga atcttatgat
    34741 taaagaggtt gtgacaagac ttttgtcaca gccttttttg tttataaaaa taaaagttgt
    34801 agtggaggct acgaagttta ctttgtcttt ttacacttaa tatttttttg ccaatttgga
    34861 atcaggatgt tttgataact atcgttttca attgatggaa gctatattgt ttgctatcag
    34921 tgaaataatt atatggtaaa cagtgttctt tttcttgttt ccattataaa aatcgtatcg
    34981 ggatttttcg ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa
    35041 aatcaggtaa atagttgacg aaacagaaat aaatacatag tattatgttt tgaagcctaa
    35101 gtacaatagt ctatagaaaa ggagcgatac cattggcaaa acgcattgac cgaatttata
    35161 tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga atatgatcaa ggagtaacaa
    35221 cacaggaaat tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa gatctgaatc
    35281 agctagtgcg cgaaggaaaa ctgataaaga cagacggacg accagtacgt tacatatatc
    35341 aaacactcgt tacgcacgta aagccagtta caaaacatgt agtcagttac aaagaaacgc
    35401 cggtctctga agaaaaaaat acactccaaa tagatactaa ggatatcttt gcaaaaatca
    35461 ttggagctaa cggcagcatg aaaaattcag tggaacaggc aaaagctgcg attttgtatc
    35521 cgccaagagg attaaattgt ttgattacag gacctaccgg atcaggaaag acttattttg
    35581 cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa acggaactga
    35641 ttgttttcaa ttgtgctgat tatgccaata accctgagct tttgatgagt catttatttg
    35701 ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa aatggggatt atcgatcaag
    35761 cggatggcgg tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa
    35821 tgatctttta ctttatggat catggaactt acagtcgtct tggggaaaca acgaaatccc
    35881 atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga cccaggttct tctctattag
    35941 aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg
    36001 caaatgagaa agtcgattta gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc
    36061 gaaagatttc tttgacagaa gatgtagcaa aagcattgat cggcagtgtg acctatggaa
    36121 atataggaca attgaagtca aatatccagt tagtatgtgc acgaggattt ctaaatcata
    36181 tgcaatcacc cgaaatctcg atcacaatcg atgatttgac agaagggatt cgtagcggat
    36241 tgattcaatt agcaagcaac cgcagtgcaa tggcagagct ttctaaaata ttggagccaa
    36301 agataactgt agcaccaaat gatacgatca tgaaaatcca atcggactcc tatgagttac
    36361 cttataatct atatgatatc atcggagata aagcagcttt actgaagtcg gatggtttgg
    36421 atcaagaagc aatcaatcat ttcatttcta cagatatcaa catccatctg aagtctttct
    36481 ataaagacca tggtttttct tttaatgcag acagcaaatt agcagaattt gtagatccga
    36541 aaatcataga agtgaccaat cagatttatg taatggtcaa aagatcttta ccatatgagt
    36601 ttcagcaaaa ctttatttat gctatgagct tgcatatcag ttcttttttg aaaaaaatca
    36661 acttaggaga agaacggcat acgaatgata acatccgtga gatggctttt gattttccag
    36721 atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt caagtggaga
    36781 ttccagaaag tgaagactat tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag
    36841 caagcggcag aatcggtgta gtcgttgcag ctcacggaaa cagcacagca agcagtatgg
    36901 tccaagttgt ccaacagttg ttagatgcag agaacgtacg agcagtagac atgcctttgg
    36961 atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag
    37021 gaagcggggt catactctta gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc
    37081 aaagagacac tggaattcca gtacgtactg ttgatatggt aacaacttct ctagttttgg
    37141 aaactgtacg aaaggtctca gtattaggga cagatctaga tatgttgcat gaatcattga
    37201 aaaattttcg aggttacgca gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta
    37261 ttcataagaa aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa
    37321 tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga aacagaatta gaagttctag
    37381 ctttgtcgat tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa tatcagatca
    37441 tagctactac tgggattact gatttgaata tccaagcccc atttattcct ttagaacgtt
    37501 ttattgatca aaatatcgaa gtcattttgg atcagttgct gatggaatca gaattggagg
    37561 aaactgaatt catctcccta gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct
    37621 ctgataactt catctttatt aatggtagta agttaatcga tccaatgtgg caattttcta
    37681 cacaaatctc tcagacgacg ggaatcggcg atgaagaata tggattcaaa atcaacctag
    37741 tgatgcacac agcaggaatg atcgaacgta ttattcggaa tgaaccattg actgtggaag
    37801 aaaacgaatt gacaaataca acgaatgatc ctctttatag ccaattggca gcctcagttg
    37861 ttctgttgga agatcagata aaagtaaaag tgccgataga agaaatgtat tatctgctac
    37921 gtctagtcca taatcagtta gataaaaaag aatacactgt gccatgaata cacaaaaacg
    37981 atacactttt atagtgtatc gtttttgtgt attggttaag aatcctttaa attcaattag
    38041 tgtatcgtaa aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg
    38101 ttcacacaga aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg
    38161 gatattcgat tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg
    38221 tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa
    38281 ttgagaaagt ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc
    38341 gtgcagaaaa tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt
    38401 ttattcacca atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg
    38461 ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca
    38521 gtaaatcaac aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag
    38581 attcgaaaag tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa
    38641 aaggtgtagc aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa
    38701 ttattaatat ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg
    38761 attatcctag caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc
    38821 tttggcgaac aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac
    38881 ttgagagcca aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc
    38941 ttagttgatt tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac
    39001 aaagataaat gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca
    39061 tcacgctttt caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa
    39121 gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt
    39181 gaagacgcac aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc
    39241 aaatatgtat tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg
    39301 acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat
    39361 ctgcgtaaga gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct
    39421 attagcaaaa tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt
    39481 ttatttgaaa atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa
    39541 ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct
    39601 atgggacaag aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat
    39661 gtacgtaaag tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa
    39721 gctgaattag caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta
    39781 ttatttcaat aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg
    39841 atgaatttca atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta
    39901 acctcgaagc tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa
    39961 atatcggtgc cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt
    40021 tagttttagg tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt
    40081 tggctgttgc agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc
    40141 atttgatgga tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca
    40201 ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc
    40261 cagcacaaac tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg
    40321 ctatcggtgg gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta
    40381 caaaagaagt atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa
    40441 cattgattgc tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta
    40501 aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca
    40561 ttttgaatga ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt
    40621 atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa
    40681 ctacgaacgt atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt
    40741 atacaaaaca aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac
    40801 acacccatac attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc
    40861 gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc
    40921 tttagctggt gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact
    40981 aggtgcttca ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg
    41041 gaacattatt cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc
    41101 taaaattact gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat
    41161 tttagggatg ttcgtattag ctgcccttgt agaaagatgg gtatcaatca aattcttacc
    41221 agttgtatca actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc
    41281 aggtggtgaa ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc
    41341 tgaaaaggta acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt
    41401 aggattaaca ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat
    41461 ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa
    41521 aatatagctc gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata
    41581 tctggaggga gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta
    41641 cttcgtttac gggtctgaca gattacgggc aaatcatgat aggtgataat ggttttgagt
    41701 tttaccatgc gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg
    41761 tactagcatc agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa
    41821 aaaatggtac atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag
    41881 aatatgtaga tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg
    41941 gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt
    42001 ctttattttt ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct
    42061 ttaaatccca tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat
    42121 ggacattgat tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa
    42181 attcgatggg ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag
    42241 cgattttcgg cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag
    42301 gcgcagtgat tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta
    42361 caaaatagta aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat
    42421 ttaaaataaa aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt
    42481 tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag
    42541 aaaaattgcg caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt
    42601 acaatcttcg ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg
    42661 aaaaagcatt ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag
    42721 ggtttgaaat tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca
    42781 aagaagaact gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg
    42841 ttttagaaga aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca
    42901 gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata
    42961 tggcatatga tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa
    43021 atcaactaga cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg
    43081 ttgctagcgg attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac
    43141 aaggtgactt gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga
    43201 cacgtacatt cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct
    43261 tagatgctca attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag
    43321 atgcagttgc tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta
    43381 caggacatgg gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa
    43441 agcaatttgt accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg
    43501 gtggagtacg tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc
    43561 gttcaccaaa agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat
    43621 cagtgttcac agactttgtt atagaaaatc actcgattta atgatacact atagaagaac
    43681 ttttaagctg taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc
    43741 tgatcggcgg cagttttctt accacgtgat cactatgccg catcatagag aattcggatg
    43801 tatctcgtgc ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt
    43861 aaacgcaaac caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat
    43921 cgggattgca gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa
    43981 tgtcactgaa ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga
    44041 agatggcttg aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt
    44101 agcaatggaa atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt
    44161 atctgaagtc aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat
    44221 cgaagaactt tttgaagacg aagaggaaga gaatgaataa agaattatca agacacgaga
    44281 ttcgggaaat ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag
    44341 aagatgcgat tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat
    44401 cagagtttgt tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg
    44461 aattggataa agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa
    44521 aaatggattt ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac
    44581 cagcggctgt tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt
    44641 cacgtaaatt cgtcaacggt gttttatcca atgtattgaa agaattagaa gctggggcgt
    44701 aagtcctagc ttttttctat gaatcatcat aaacctgtta taaatcggga aaattctatg
    44761 gtagaatgaa aacacaaaaa atatctggga gtggttagat ggcagaattg attaacggaa
    44821 aagaattagc tgaaaaaatg caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata
    44881 atggtatcca tcccggatta gtcgttttat tggtagggga aaatccagcc agccaaatct
    44941 atgtaagaaa taaagaacgt gtagcaaaag aaatcggaat ccattcattg gtagagcgtt
    45001 atcctgagac gatttctgaa gacgaactac ttgcagaaat tgaaaaatac aatcaagatc
    45061 ctagttttca tggtatattg gtccaattac cattgccaaa gcatatagat gaagaaaaga
    45121 tcctgttagc tatccgccct gaaaaagacg tagacggttt tcatccgatg aatttaggca
    45181 gactccttgc gggtaaaccg gacaagattc cttgtactcc atacggcatc atgaaaatgt
    45241 ttgaagctta ttctatcgat ccggcgggta aacgagcagt agttatcgga cgaagcaata
    45301 tcgtcggtaa accgatggct cagctactct tgatggcaga tgcaactgtt actgttgctc
    45361 attctaaaac cgaaaatctg gctgaactgg cacaagaagc agatatttta gtcgtagcaa
    45421 taggacgtgg acattttgtc acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg
    45481 tagggatgaa tcgtgatgct aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg
    45541 agcctattgc aggctggatc actcctgtgc caaagggtgt agggccaatg actatcacga
    45601 tgctgatgta tcaaaccgta aaatctgccg aagcttttgg agcaggtgaa tagatggcac
    45661 aagaatattt aactgtcact acattaacga agtatttgaa aagaaaattt gatgccgatc
    45721 cttatttaga acgcgtatat ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg
    45781 cccatcaata tttcagctta aaagatgacc atgcaaaaat atctgctatt atgttcaaag
    45841 gagcattcca aaaattacga ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc
    45901 gtatttcatt atatgaagcg ggaggttctt atcaaatcta tgtagagcac atggagcctg
    45961 atggaatagg tgctttatat caagaattgg aagaacgaaa agaaaagctg atgaaggaag
    46021 gcctttttca agggcctaaa aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta
    46081 ccagtccaag cggagcggtt atccgcgata tcatcacgac ggtgaaaaga agatatccaa
    46141 ttgtccaatt ggttttgttc ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg
    46201 tcaggaatat ccagagagta gaagagatgg gcaatttcga tacaatgatc atcggtcgtg
    46261 ggggtggttc gattgaggat ttatggccgt ttaatgaaga gcgggttgct cgggcgattt
    46321 atcaagctaa tacgccggtg atttcatctg taggacatga aacagatacc acgatcgctg
    46381 atttagtagc agatgttcgg gctgccactc caacagcagc tgcagaatta gctgttcctg
    46441 ttttgtcaga agagttgatg cgcattcaag aacggcaagc tagattgcag caggcgtttg
    46501 tacgccagat ccagcgcaaa caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc
    46561 ggcagcctga aagattgtat gaaggtcaga ccatcaaatt agaccagttg aaacagcgat
    46621 tatatcaagc tacacagcaa atttatcatg cgaaagaaaa acaaacgatc gcattaagcc
    46681 accaattgga acaagtggct ccgctttatc gagttaaagc agctcgccaa gaaactgctt
    46741 atctagaaaa acgcttagaa gagaaaatcc aacaatatat gaaacaacag cagcagcgtt
    46801 ttgctcaagc tgttcaatct cttgatttgt tgagtccatt gaaaatcatg ggaagaggct
    46861 acagctatac gacgttaaat gatcaagtag taaaaagtgt caccgagatt catcaagggg
    46921 atttgctgac gatccattat caagatggaa tcgttaaagg acaagtacaa gagatagaca
    46981 aaggagaata aaaatatgga aaatccaact tttgaagagt cactgcaaga attagaaaaa
    47041 attgtcatgc agttagaaca aggagacgtt ccattagaat cagctctaga ttcgtttaaa
    47101 cgaggaatgg aattaagcaa acattgccaa gatacattag ctaaagcaga agagaccttg
    47161 acgaaaatga tgacagaatc aaatgaagaa gttgattttg acggaaacga ggaaagctga
    47221 gatgagatta actgactttt cagctattca tctacctgct gtagaaaaag aaatcttgtc
    47281 ttttttagat gagcatacaa cggatagagg actctatgat gcgatgagtt attcagtgaa
    47341 agcaggcggg aaaagaatac gtcccttatt acttttaacg gccgttgctt cctttgatga
    47401 gcctattgat gttcccgtct atcaagtagc agctgcttta gaaatggtgc atacgtattc
    47461 cttgatccac gatgatttgc ctgcgatgga caatgatgat cttcgtagag gaaaacctac
    47521 caatcataaa gtattcggcg aagcattagc tatattggcc ggagatggtt tgcttacagg
    47581 agcattccag ctaatcagta tggcacactt aggtaattct cctaagcttc tgctgcttca
    47641 gcaattagct gtttgtgcgg gatcacaagg tatggttgct gggcaagctg cagatatcga
    47701 gggcgaatcg aaaaagcttt ctttagaaga attagcattt attcatgaaa gaaagacagg
    47761 gcatttgact cgttatgcct tgctggctgg tggcatatta gcgaagcaac ctgaagaaat
    47821 cttattgcta cttcaacgat tagccgaaca tttaggttta gcttttcaga taagagatga
    47881 tctactcgat gtgattggta caacgaaaac attagggaaa acagctggta aagatgaacg
    47941 tatggaaaaa aatacttacc cgcgtttatt aggcctagaa aagacacgtg aggcgttgga
    48001 gattgagctg ttatccgcca acaagatcat agacaaatta gaagaggacg ttttttcttt
    48061 tgatggcggg ctgatgcgcc agatgatcaa gcaatttgat gtggagaaat gagggaaaga
    48121 atggaaaaag aacgcgtaga tgtattagcg gtcaaacaag gattatttga aacaagagaa
    48181 caagccaaac gttcagtgat ggctggtttg atttacaacg aaaaaaacga gcggtttgat
    48241 aaaccgggag aaaaaattcc agtgagtagt gaattaagag tcaaaggtaa aaaacttcct
    48301 tatgtttctc gagggggatt aaaattagaa aaagcactca aagcatttga tctatctgtt
    48361 gaaggaaaaa cattgttaga tattggtgca tccactggcg gatttactga tgcagctttg
    48421 caaaatggcg caaaaatgag ttatgcttta gacgttggct acaatcagct agcttggaaa
    48481 ataagacaag atcctagagt cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc
    48541 gaagacttta ccttaggaat tccagaagtt gctgttatcg atgtttcttt tatttcacta
    48601 aaactgatgc tgcctccatt acatgctatt ttaaaagaag agggagaagt gattgcttta
    48661 atcaaacccc aattcgaagc aggcagagaa gctgtaggga aaaacggaat tgttcgtgac
    48721 cctcagaccc atcaaaaagt tttagaagac attttgtcat ttgcggcaca aggtggatat
    48781 gatgtgctgg aattaagtta ttctccgatc acggggggag aaggaaatat cgagttcttg
    48841 gctcacttga ggaaagtacc cgaaagcgga acaatcaatt cagctattaa tatggcagaa
    48901 gtggtttcaa atgcacacga acagtttgac cataaataaa ttgtaactgt gaaaatcaag
    48961 tgagaagtat gcggattctt ctttattata ccaaggaaat aagctatcat tgattaagaa
    49021 gaatatgcta aggagcgaag acgtaatgag aaaaaaagat cggcatcgtt tgattactcg
    49081 attgttgagt gagcaagata tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa
    49141 aggaatatct gttactcaag ctacgatttc cagagatata aaggaactga aactgatcaa
    49201 ggtaccggct tcagatggcg gctaccgtta tagcatacca gctgaaacaa acgaagatgt
    49261 aaatactaaa ttggaaaaac tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa
    49321 atttgttatt ttgaaaacat tgccgggaaa tgcttcggca gcagcaaatt tgattgataa
    49381 acgttacaag aaagaattat tttcgattat caatgatgac gataatgttt tgatgattac
    49441 acgaactgaa gaccaggcag ttttattgaa gaaagacttc ctgcattacc tttaagttga
    49501 ggagcgaatg tgatgctaca agaaatcagt attactaact ttgcgattat tccggaattg
    49561 cggctttctt ttcacgaagg aatgaccgcg ctgaccggag agactggtgc gggtaaatcc
    49621 attattatcg atgctttagg tctgttagca ggaggaagag gttcaagcga ttatatacgt
    49681 caaggagcag aaaaatgtgt gcttgaaggg ttgtttgaac taccaaaaca agaaggattc
    49741 tctgaactga tggttgaatt agggatcgaa acagatgagg acaatctgat tgttcgacgg
    49801 gatatgtcct taactggaaa aaatgtttgt cgagtgaatg gtcatattat cacattagct
    49861 aatctgagaa gaatcggcag ttatttagta gatatccaag gacaaaacga acatcaggaa
    49921 ctgctccagc ctgaatcaca tcttgcttta ttggatcgtt ttggagatgt agctttccaa
    49981 cagaagaaaa agagttatca gcaagaatat atctcttatc gtgagctgga aaaaagggta
    50041 cgaaaagtgc agcaaaatga aaaatcctat gttcagagaa tagatatgct ccatttccag
    50101 caagaagaaa tagctgctgc tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa
    50161 agagaaaaac ttagcaatta tcaaaaaatt gctgatggat tagctgcagg ttacggagca
    50221 ctgacgaata atgaacaaaa cagtgtagat ggtgttggct tggccgtttc agaactgcaa
    50281 gggatcgccc atctagatgt ggagtatgaa gcaatttatg aaaatatcca aagtgcttac
    50341 tatttgctgc aagatgcaat cggcgatatg agtcgtcaga ttgatttatt ggagttagat
    50401 gagagtcgtt tagaagaagt tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa
    50461 tacggcgaat ccattgagtc aatattggct tactatgacg aaatcaccga agagctagct
    50521 tcttctgatt tttcagaagg tcaattggac aaaatgaaag aggaactaga gcaaaaagag
    50581 cttctacttc agcaacaagc cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa
    50641 ttggaaaaat ccattctgca tgaattgaaa agtctgtata tggaaaacac tgagtttgag
    50701 gtacgtttct taaaagaaga aaatagacaa ttgaatcatg atggatttga ccaaatcgaa
    50761 ttttacatta cgaccaatcc aggagagcca ctgaagcctt tggtgaaagt cgcttctggt
    50821 ggcgaactgt ctcggatgtt gcttgctttg aaaactattt tttcttctga gcaaggtgtg
    50881 acaagtatta tttttgatga agtggataca ggtgtgagcg gaagagttgc acaggccatc
    50941 gcagataaaa tttctcaaat atcgaaatac tctcaagttt tgtgcatcac tcatctgcct
    51001 caagttgctg cagtcgccga ctatcaatat tatattgtga aagcagtgat tgatggacgt
    51061 acgcaaactt ctgtttcaga gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg
    51121 ctggctggaa gtgagattac aaaactcaca ctcgaacatg caaaagaact attgaaactc
    51181 gcaaaaagga cctgagccac ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat
    51241 attttatttt tacatgacac tcattacagc agtcaataca acagatccaa tcgttaaaat
    51301 cagcattgtc caaatgacaa attttgttac tttagcaaaa gtacttgttt gttttttttc
    51361 acgcattgtt ttcaacctcg tcattaatta gttgattcaa gtgtacaatc ttttttccat
    51421 ttccacaaga gggctttttt ataaaaactt cccttttttt aaataatagg tagttacgat
    51481 acaaataaat aatgttaata gaactagcca tataaaggca tcatcccttt cagcaatagg
    51541 taacttaatg ttcattccat agatactgcc gacgattgtc ggtatagtta aaataatcgt
    51601 taaagaagtt aaaattttca taatattatt cagattattc gatacgattg ctgaaaaagt
    51661 ctgattcatt tgttctacta gttttaattg aatgcgagaa gtagtcattg cctgctctgt
    51721 ttccaccaat acatcacgta aatgattttt caaagcatga ttttcttgga aaagtttatt
    51781 ttgcgctagt tctttcaatg catctaaatt agcagaagtc gcttctctga aataaacgag
    51841 gcttttttgg atatccatga tttgataaag ttgtttgttt tcagtggata cttgaagttt
    51901 ggcttccaaa gtatcgcatt gagattgaag cgtagctaaa taactgttgt aagagagtgt
    51961 gacctgccaa atgaattgaa agaagatatt gagattaggc gaaatatcac tttttggaaa
    52021 ggtctggcta aagactttct ttaaaaacag tggttcatga ttcgtaattg tgataagctt
    52081 ctttttgggc gtcaaaataa tagagagcgg gtatgtatcc acttgaaaat aaccgctagg
    52141 acttgtttta gcaaaaggat attgtaacaa taataacgct ggctttgtca actttgattg
    52201 attgaattct tctgtacggg aattttctgc atcgtccaat atggaagtga tgtaatctga
    52261 cggaatttca aattgatgtg tcagctgatg aatctcttct tgattaggac gttctacaca
    52321 ataccagatc gtttggtcag tttcctttcc ggtattacgc agtttctcat tatctaatgt
    52381 aaaatatttg atcatgattt tcctcctgtt gcctgtttct tttattataa cggataggga
    52441 attggaatac atggaatgtg tttgtaagat atagaatata actgcataaa tttaaataaa
    52501 atgaaacagt tgtgtaaagt atatacagta tacttgaagc aacgataaaa aaggagttta
    52561 cgttcatggg cacattcaca ccacttatct tgttttggta tatttacccg gtaatcgttt
    52621 tgtttggttg ccaatttctt gtaaaattat tttcgttaaa tcgtcgtttt aaaataaaag
    52681 cccctgattt ggcgattcct tttttgtggg gcggtatgca tgcattgtct agaaatacag
    52741 ggacaatttc tatcttaccg tttttcttta tttctgtttt gctaatggga atttgtattg
    52801 ctgttttcca agcatattat tatgaagaaa ttatttatcc gcgttatttc aagatgactt
    52861 ggcgtttggt ttttctgttg acaatcgtct tgtacgctgt cttgatcatt gtgaacatac
    52921 tttcttacct atgaaaaaaa tagaaaagct gtaaaaagtc ttttaaaagg ctttttgcag
    52981 cttgattttt aaaaaaatgt ttatgaataa aaaaagttgt tcccggattt cccagaaaag
    53041 gtggtagaaa gtgggggatt gtggtagact aattttagtt agtggaggaa tggggggctt
    53101 tcaatgttga tgggtgaatt ccaacataat attgatgcaa aaggacgttt aatcgtgccc
    53161 tcaaaacttc gtgaagaact tggcgaaaag tttgttctga cacgcggatt ggatggctgt
    53221 cttttcggct atccgatgag cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg
    53281 ttggctaaaa aagacgcacg tacttttgtg cgattcttct attcggctgc cactgaatgc
    53341 gaacttgata aacaaggacg aatcaatata cccagcacgt taagaaatta tgcagctttg
    53401 acaaaaggtt gcgtagtcat cggtgtatct aaccgaattg aaatttggga tgagacacga
    53461 tggcaagatt tttctgcagc agcagaagag aactttgacg aaattgctga aagtatgatt
    53521 gatttcggac tataagaata ggaagaggct gactaatgac tgaaacattt caacactata
    53581 cagtcatgtt gaaagaaacg gtagatggat tgaacatcaa accggatggc gtttatgtag
    53641 actgtacact cggcggtgcc ggacatagtg aatatttatt aagccaacta agttctaaag
    53701 ggcatctgta tgcgtttgac caagatcaaa aagcaatcga tcacgccaag ctgcgtttag
    53761 cttcttatgt cgaacaaggt atggtcacat ttatcaaagc gaacttcaga gaattagaag
    53821 aacgcttgag tgagcatgta gataaggtag acggcatttt gtatgatcta ggggtttcct
    53881 ctcctcagct agatgaagca gaacgtggat ttagttatca tcaagatgca ccgttggata
    53941 tgcgcatgga tcagtcagct cctttatccg cttatcacgt aattaatgaa tattcctacc
    54001 atgaattagt aaagattttc ttccgctatg gcgaggagaa attttccaaa caaattgctc
    54061 gtgaaatcga acgagtacga aaaacaaaac caatccaaac aactggtgag cttgtcgaaa
    54121 tcattaaaca agtcatccca gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa
    54181 tttttcaagc tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag gcctctttag
    54241 aacaggcaat ccggttattg aaagtcaatg gaagaatcag cgtaatcaca ttccattcat
    54301 tggaagacag aattgtcaaa agtatgttta aagaatatag tacggtacaa gatctaccac
    54361 cgggacttcc tgtcatacct gacgaattcc aaccagaact gaaagtcatt actcgtaaac
    54421 caatccttcc ttcagaggaa gagctaacag aaaacaatcg atcacgcagt gcaaagttac
    54481 gtattgcaga aaaaataaga atgaaagagg agtagaatga tggcagaatt aaagaaaatg
    54541 caagattacg aatatgattt acctgaattg gtggatcagc cggaacaaca aatgcctgat
    54601 tcttcttcga atcaagaaat cattcttcca caatcaccgg caaaacgatt aaagcagatt
    54661 tctgctttgg aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt
    54721 ttaacagtta tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa
    54781 acagaaatca atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt
    54841 tctcgtactg aacggattaa acaaatcgca gaagaaaaag gattgtctat taatgacgac
    54901 aatttaagga aagtgaagta aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag
    54961 aaaaatctaa atccgatgaa caatcgcaaa aaagtaggta tcattttatt tgctacgagt
    55021 attggaatgt tctttttatt tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt
    55081 gctggtacct cattagctga aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa
    55141 gcaaaaagag gaacgattta tgatcgtaac ggtgtggcgc tcgcagaaga tgcctcctct
    55201 tattcaatta aagcgatact ctcaaaaaca tatacctctg gggataaaaa actgtatgtt
    55261 gaagaaaaga attttgataa aatagcagaa atcctgcata agaatctttc tatagacaaa
    55321 aaagatgcac tgaatatttt agaggacggc gcaaaaaaag agctgtatca agttgaattt
    55381 ggtagctatg ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa
    55441 aaagaaggtg tcgctggtct ttattttgtc gaccatcagg ctcggatgta tccaaatggc
    55501 gtcttttctt ctcattttat tggatacgca gttccagaca aagatgagaa tggcttagtt
    55561 ggtaaactag gtttggagtc tgcttacaat gatatcttga gcggtaaaga tggtaagatt
    55621 atttatcaaa aagataattt tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa
    55681 gctgtcgacg gacaagatat ctacacgact ttagacagca gactacaaag ttatttagaa
    55741 acactgatgg atcaagtaaa tgaagaatat cagccagaag aattgacagc agtcctgatg
    55801 aaagcaaaaa ccggagaaat cttggcaatg ggacaacgac ctactttcaa tcctgaaaca
    55861 atggaaggat tgactggaaa agatgctatc tggcgtaatt tcttggtaca agatagttat
    55921 gaaccaggat caaccatgaa agttttcact actgcagctg ctatcgaaga aggagaattc
    55981 aacgagaatg aaaccttcca atctggaaaa atccaagtag aagatgctac gatcaatgac
    56041 catgactttg gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac
    56101 gtcgggatgg tcatactgga acagcgatta ggcggacgat ggtataacta tttacaaaaa
    56161 ctgggatttg gacaaagtac tcattctgga ttagatgatg aagtaaacgg tgctttacct
    56221 acattaaata ttgtcgaccg agcaatgagt gcttatggac aagcggtagg tgtgacgaat
    56281 ttccaaatga tgaaagcttt cacttctatt gcgaataacg gtacaatgat tcaacctcga
    56341 tatatcagca aagtggtgga tccccaaact ggagaagagc gaacaactca aacagaagtt
    56401 ctagggcaac ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt
    56461 gtcgaaagcg aaaactacgg tagtgcttat ggtgtatata gtgtacctgg atacaatgta
    56521 tcggcaaaaa ccggaacagc ccaaatcgct tctgatacag ggggatatca aacaggagat
    56581 acagcctacc tgtattcgat tgtggagatg gttccttctg aagatccaga ctatgtcttg
    56641 tatctaacaa tgaaacaccc taagacatat gatcgaatgg cattggctaa aatcgcaaat
    56701 ccattgatga aacgggcaat ggattttaaa gaaactgaag aagattcaga tacagaaaca
    56761 aaaacagaaa aagtatccgt agccgattat cgtaatcttg aagcggacgt tgcagcagct
    56821 gatgctcaaa aaagtggact gcaacctgta gtgatcggta atggtaaaaa agtacaaaaa
    56881 caatctactg ccaacggtga tcagttgatc tctggagaaa aactgatcct atatactggt
    56941 ggtgataagc tgatgcctga cgtaacagga tggtcaaaag cagatatcat gaagctgggg
    57001 aaaatcctcg gtattgaagt tagttttgat ggagatggtt attgtgtcaa acaagaactt
    57061 gctccatatg aaaaaatcac tgaagacaaa cttagtttta ctttagaaga ataagaagag
    57121 aaataggaga gaaaagagca tggaatggac acaagcattg attcccattg tcagcagttg
    57181 tgcaatgaca attgccgcaa tgccgctatt tattggttat tttcaaatga aaaagcaagg
    57241 tcaggcgatt cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa ctcctacaat
    57301 gggcggctta gtgtttctga tcggctctat tttgacagga atttgggttg gagcttggca
    57361 aaaacagcta actcctactc ttttcatctt gctttttgta ttagcgttat acggagtaat
    57421 cggctttctt gatgatttta taaaaatatt caagaaaaga aacatgggac tgaactcgaa
    57481 gcagaaattg attggacaga ttctaggggg aatcatcttt tatcttgttt accgttctga
    57541 aggttatcca ggtgtattga atttcttcgg actagatcta cctttaggaa tcatttatgg
    57601 tattttcgct ctcttctggc ttgttggatt ttcaaatgca gtaaatctga cagatggcat
    57661 cgatggatta gttgctggct taggaagtat ctcttttgca gcttacggtc taattgcttg
    57721 gcatcagcat cagtatgatg tattaatcat ttgcttaagt gttttaggag gtttactggg
    57781 tttcttcgtc tacaacagaa aacctgccaa gatttttatg ggagatgtcg gttcattagc
    57841 gttgggcgga cttttagcgg caatttctat aatgctaaac caagaatgga cattgctttt
    57901 gattggtttg atctatgtta tggaaacagc aagtgtgatg cttcaagtta cttcctttaa
    57961 gcttacagga aaacgaattt ttaaaatgtc tccaattcat catcactttg aaatgtgcgg
    58021 atggtcggag tggaagattg atacagtttt ttggttgatc agcattgtga cttcgttgat
    58081 cacattatgg atcgtttggt aggagatgac gggaaatgaa aaaaatatca acatatgaaa
    58141 ataagaaagt gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc aaattattac
    58201 atgaattggg cgcacttgtc accgttaatg atggaaaacc ttttgacgaa aatccggaag
    58261 cacaagagct gctttcttta gggataaaag taataacagg tagtcatcca atcgaattgt
    58321 tggatgaaga attttcattg atggtcaaaa atccagggat tccctacagt catcctttag
    58381 tagcaaaagc ccaagaaatg ggtattccgg taattacgga agttgagcta gcttacgaag
    58441 tagcggaatg cccaattatt gggattacag gaacgaacgg aaaaacaacg acaacaacga
    58501 tgacaggact actactgaat gcaggagcag atcaaggaat tgcacgttta gcgggtaata
    58561 ttgggtatcc agcaagtggt gtagcgcaag aagcaaaatc agaagataaa attgtgatgg
    58621 aattgtctag cttccaactc atgggaatca cagatttccg ccctcatata gcggtgatta
    58681 ccaatatcta tgaagcacat attgattatc atggtacaag aaaagaatat gtcaaagcta
    58741 aatggaacct tcaaaaaaac atgacagaaa aagattatct gatccttaac tggaatcagt
    58801 cagaactaca agaattagcg caaagaacaa aagcacgcgt actgcctttc tcgacaaaag
    58861 aagtgttgga agacggtgtg tatgctgacg attactcaat atactataaa aaagaaaaaa
    58921 taatggaaat atctgaactt ggcgtaccag gtaagcataa tgtagaaaat gcactagctg
    58981 ctatttctgt agcaaaattg tatggtatat caaatgaagc tatacgagaa acgttgcatt
    59041 ttttccatgg cgtaccacat cgtacgcaat atgtaggaga aatccaaggc agaaaatttt
    59101 ataatgattc aaaagcaacc aatattttag cgactaaaat ggctctaagt ggatttgaaa
    59161 cgtctaaggt cgtgttattg gctggcggat tagacagagg aaatacattt gatgaattaa
    59221 ttccttcatt aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa gaaaagttga
    59281 tggatgctgg aaaaaaagca ggcatcgaga caatagtaac agccgattct gtagaacaag
    59341 cagttccttt agcattagaa aacagtacag atggtgatgt agttttatta tcaccagcga
    59401 atgccagctg ggatcaatac ccaaattttg aaactagagg aaacagattc atggaagctg
    59461 tgaaccgatt aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat tttggttaca
    59521 ggcggaggaa cagggggaca tatttatcca gctcttgcct ttgtcaatta tgtaaagact
    59581 caagaaccga atgctgaatt catgtatgtg ggagcaaaac gaggtctaga aaacaaaatc
    59641 gtccctgata caggaatgcc tttccatacc ttggagattc aaggatttaa acgaaaaatt
    59701 tccatgcata acatcaagac ggtccgattg tttcttaaga gtatccgtca agcaaaaaaa
    59761 atcctcagtg attttcaacc agatatcgtt atcggtactg gaggatatgt ttccggagcg
    59821 gtagtgtacg ctgcagccaa aatgggtatt ccaacagtta tccatgaaca aaacagtgta
    59881 ccaggtgtta ccaataaatt tttaagccga tatgtggatc ggatcgctct ttcatttaaa
    59941 gatgcggctt ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc tcgagcacaa
    60001 gaagtagcag atactaaaaa atcagatatt ttgaaaacat ttgatttgga tccagaaaag
    60061 aaaacagtct tgatttttgg cggtagtcaa ggagcactta aaatcaatca agcagtatca
    60121 gtttttttaa gtgaatatat tccttcttct taccaagttt tgtatgcttc aggagataga
    60181 tattatgaag agatcctaaa taaaataaat cctgaaatac aaaatattag tgtccagcct
    60241 tatatcaaaa atatggcaga ggtcatggct tccagtgatc tgctggtggg gcgtgctggt
    60301 gcaacttcga tcgcagaatt cacagctctg gggttaccgg cagtgttggt tcccagccca
    60361 tatgttacga atgatcatca gactaaaaat gcaatgagtc ttgttcacgc aggagcagca
    60421 aaaatgattg cagataatga actaacaggt gaatcactaa gtcagacagt caacgaaatc
    60481 atgggtgatg aagaattgca aaagcaaatg tgccgagctt ctaaagaaca aggaatccct
    60541 gatgcttcaa aacgtcttta tgacttagta aaacaaataa tcaaaaaata aaggcggtgg
    60601 tcttagtgag taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct gatgaaaaca
    60661 atttgactcc ttggcaaaag gctaaccgaa aatatctggc agaacaagag atgaaaaacc
    60721 aagagccaaa aaaagaagac ataacattag atacacccct ttcagattct gaagaagaaa
    60781 aaacggttca agaggaacag caagaagaaa gccagtccga gaaaaaaatc gaattttttg
    60841 aagagatcaa gcaaaagaaa aacggtggac catataacgg ttcttttttg aaccggctgc
    60901 ccaatttaaa aagtcaacgg cacaaagtac tatttcgtcg gttagctttg atcatcactg
    60961 tattgggtat accgctgatt tttttgatct attatgtttc tccttatagc aaattacaag
    61021 cagttgcagt atcaggcaat aagacagtca acagccaaga aatcatctct gatacaaaat
    61081 tgtcattagg agaaaatgtt tggggacagt attttcatcg atctacttat atcgatcgcc
    61141 ttaaaaaagc acagccaaga atcgaaacag caaatataca tttcaaaggg atgaatgaat
    61201 tcgatttgga tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac aatcaatact
    61261 acccagtgat tgaaaatgga acagtattag atgaaaaagt agcaaatcct acaaaaaatc
    61321 tccctatctt ggaagagttc aaagactctg caaaaatcaa agaattaaca aagcaataca
    61381 atcagttatc atcagaatta caaaaagcaa tttctgaaat caagtacacg ccaagagcaa
    61441 gcaataaaaa tctgatccaa ttgaatatga atgacggcaa tcaagttatt gtaaatatca
    61501 ataatcttgc gaaccaaatg aaatattatt cgcaagtagc caaagatatg gatgaaaaag
    61561 gtgtgatcga catggaagtt ggtatcttct cgcatcctta cggtagcact caagaaacgg
    61621 aagaatcggg ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat caaacaaacg
    61681 aatcaagtga agaggcagaa acgcaaaaag cacaaacacc tggatcgtct agcacaaacg
    61741 gatcatctga ttcaaatgat tcaagtgaaa caagtcaaat agaagataat ccaagtacaa
    61801 ctccgtcaag tttaaatgaa aattgataaa acaactttaa aaagtaagaa tgtactttct
    61861 aaaaaccagt aatttatggt agaattggaa gcagtgaata tatctattaa taagtatatt
    61921 ttaaaacgga cgctagttgt tcgcttgtat atggaatagt aagtaggagg gaatcccatt
    61981 catggcaaaa acaggaatgt atgtaggcct tgatattgga acgacatctg ttaaagttgt
    62041 cgtggctgaa tatatcgaca gccaaatgaa tattattgga gtaggaaacg caaaatcaga
    62101 agggattaac cgaggcatta tcgttgatat cgataaaacg gtccaagcga tacaacgagc
    62161 agtacgacaa gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg tcggtttgcc
    62221 agcaaacatg cttgaagtag aaaattgcca gggaatgatt gcagttaacg gagattcaaa
    62281 ggaaatcacc gatgaagatg tcagaaatgt ggcttcagct gcccttgttc gttcaatacc
    62341 tcctgaacgt caaatcgtgt caatattgcc acaagatttt actgtcgatg ggttcgaggg
    62401 aatcaaagat cctcgtggaa tgatcggcgt aagattagaa atgtacggtc tgttgtttac
    62461 aggacctaaa acgatcatcc ataacatacg taaatgcgtg gaaaatgccg gattaatcgt
    62521 caacgaaatg gtgattactc ctttagcgct gactgaatca attctttctg acggagaaaa
    62581 agattttggt acgatcgtca ttgatatggg tggcggccaa acaacaacgg ctgttatgca
    62641 tgataagcaa ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg tcactaaaga
    62701 tatctctatt gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa taaactacgg
    62761 ggatgcatac ccagaacgga catcaccaga tgaagaattt ccagtagatg tgattggtca
    62821 gtcagaacca gtgaaagtcg atgaacgcta cttgtcagaa attatttcag ctcgaatgga
    62881 acagattttc aacaaagcaa aagatgcatt ggaccaaatc gaagcattag agttgccagg
    62941 tggcgtcgta ttgacaggcg gagctgctag tcttcctggt gttgtggact tggcacaaga
    63001 aatcttcggc gtaaatgtaa aattgtatgt tccaaatcac atggggctga gaaacccggt
    63061 atttacaaat gtaataagta tcgtggatta ttcagcaaac ttgagtgaag tgtatcaatt
    63121 ggcaaaaatt gctgttacgg gtgaaacctc agcagctcgt caaatggtag ttgaacaaga
    63181 aacaacaaac acatatgaga gctatgaagc acctgaagaa acaatctacg atgagccgga
    63241 accaaaagag tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta caaatatttt
    63301 tgattaattc atcctgggag gaataagata tggaattctc aattgataat aatatcaatg
    63361 acggcgcagt aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct gtaaaccgaa
    63421 tgattgaaga aaacgttaaa ggtgttgaat tcattacagc caacacagac gtgcaagcat
    63481 taaaaaattc aaaagctgaa acagttatcc aattaggccc caaatatact cgtggtttag
    63541 gtgctggttc tcagccagaa gttggacaaa aagcggcaga agaaagtgaa caatccttac
    63601 gcgaagcttt agacggtgca gatatgattt tcatcactgc aggaatgggt ggaggaaccg
    63661 gtacaggtgc tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca ttgactgttg
    63721 gtgtcgtgac acgtccattc acttttgaag gaccgaaacg tggccgtttt gctgcagaag
    63781 gtatcgctcg tttgaaagaa aatgtggata cattattaat tatctcaaat aaccgtttat
    63841 tagaagttgt agataaaaag acaccgatgc ttgaagcttt ccgtgaagca gataatgttt
    63901 tacgacaagg tgtacaaggt atctctgatt tgatcacagc gccaggttac gttaaccttg
    63961 actttgctga cgtgaaaaca gtgatggaaa accaaggtac agcattgatg gggatcggtg
    64021 tggctagtgg agaagatcgc gtagtagaag caacgaaaaa agcaatctcc tctccattgc
    64081 ttgaaacatc tattgatgga gctgaacaag tacttcttaa catcacaggc ggtttggata
    64141 tgacattgtt cgaagcacaa gatgcttcag atatcgtagc aaatgcagca actggcgatg
    64201 taaatatcat tctaggtaca tctatcaatg aagaaatggg cgatgaaatc cgtgtgacgg
    64261 tcatcgcaac aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga ccagctagac
    64321 aagcacaaat gcaatcacct gctcaaaaga ctgtattaga tatggatcaa gctaaaccaa
    64381 cttcttcaga agaagaaaac agttttggcg attgggatat cagacgtgaa caaaatgttc
    64441 gtcctagagt tgatgattcc aattttgaca atatcgaaaa aaaagaattt gatacattca
    64501 accgcgaaga aacaaaatct catggcgatg atgaattaaa tacgccaccg tttttccgtc
    64561 gtaaaaaata aaggagagga agagcacatg attgctgaca acttgcagaa aatcaagcaa
    64621 gaaattcagg attcgtgtgc tcttgttcat cgtgatccga aagaagttac attagtagct
    64681 gtaacaaaat ctgttgaatc cgctcaagct gaagaattgg ttagagctgg cgttatgaat
    64741 tgtgcagaaa accgagtaga taaattatta gaaaagaaac aagatttatt tgattttcct
    64801 gaaataaagt ggcatttaat tggtaattta cagcgcagaa aggtaaaatt gattgtaaat
    64861 gaaattgatt actttcatgc gttggacagt ttaagattag cagaagaaat ccagaagcgg
    64921 ttagagaaag aattatcctg ttttattgaa gtaaatgttt ctggtgagga atcaaaacat
    64981 ggaatttcac cagatgagct tttaccgttt gtggaatctt tagctgcctg cacaaaaatc
    65041 aaggttgttg ggttgatgac tatggcgcct aaagaagctt ctgatgagca aatccataaa
    65101 gtgtttggta cgctgaaaga attgcgtgat cttgttcaag caaaaggtta tgcccatgca
    65161 ccatgtagtg aattaagtat ggggatgagt caagactttc aaattgcgat tgaagaaggt
    65221 gcaacattta tccgtgtagg aacagcgcta ttcaaagacg aataaaggag gaacaaaaat
    65281 gccgctttta aacagaggag caattgctaa ttttttcgga ttagctgatg aggaagagta
    65341 cgaataccaa gattatcctg aaacaacaaa aaaggctgta cagccaacta aaaaaaatac
    65401 aacacagaat caatcaactc attctgtatc tgctgcgaat actcatgaaa aacaaaaaac
    65461 ggcgacttca cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga aaaaagtaat
    65521 tgaactacat caatcaccta caaaaactgt agcgaaagaa agaacagctt caagaacctc
    65581 tacacaacct aaagcaaatc ttgctggaaa aatcactgta atggaaccaa gagcttattc
    65641 tgaagctatg gcgattgcta aacgaatcat tgctggagaa gcaaccttag tgaattttca
    65701 tttagtagaa gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa cagtttatgc
    65761 actagatgga gatatcaaac gtgttggaga tgagattttt ctttgtacac cgactgggat
    65821 ggagattgac agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg atctgtaaga
    65881 ggaggaacta cgatttatac tattgcacta ttacttcatt tacttcaacg ggcagcctat
    65941 ttctatagca ttatcttgat ggtatatgca ttgctctctt ggtttccagg cgggtatcaa
    66001 tcagggctag gacgattatt agctaagatc tgcgaaccgt atatcagttt gttcgatcgt
    66061 ctgcctttga ggattggacc cttggatttc acaattttag gggcaatcct gatcttgaac
    66121 ttagccgtac aaggtttagc acagatcgtt atatggataa ctgtccattt agtttattga
    66181 ttgaagcaac aaaatcttta ttctcagaat agataggagg ggaaaatttt ggatgcaaat
    66241 gtataccagc actttcgggc agatgaacgt acatttatcg attcggtagg cgattggatt
    66301 gaacaggttc aaggacaata tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc
    66361 tatattttag aaacactgat tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga
    66421 tatgaacagg cagaaagaaa aagatgtttg attttccccg attattacga acctgtagag
    66481 gaagattttg aaacagcatt gtatgaaatc cattatccta gcaaatttgc cagcctaagt
    66541 catgggaaaa tccttggaac tttagtggga acggggatca aacgctctta ctttggcgat
    66601 atcatctcgg acggtgatcg ctggcaagta tttattgcaa atgaggtgga tcattttgtt
    66661 gctgcacaag taacaaagat tggaaaggtt gctgtacgct tggaaccaat caaatacaca
    66721 gaaatgatta tgccgaaaga tagctggaca caagaaagag gaacagttag ttccatgcga
    66781 ctagacagtg tgatctctga gatttataat atttccagac agcggtccaa gcagctgatc
    66841 gaagctggta aagtaaaagt gaactggaca gaaaatacaa gacctgattt tgtacttgaa
    66901 ctattagata ttatttcagt cagaggttat ggcagagtcc agatccaaga acttgagggg
    66961 aagacgaaaa aagaaaagta cagggtactg ttcggtgtat tacgtaaata aaataaaaag
    67021 gtggtttact catggcttta actccattag atatccaaaa taagacattc ccgacaaaaa
    67081 tgcggggata taatcaagac gaagtagatg atttcttaga tttagttgtt cgtgactatg
    67141 aggaattgac tcaaagaaat cgagaattag aaaaagcagt caaacattct gaagaaaaac
    67201 ttgagtactt caatgaatta aaagatgctt tgaaccaatc aatcatcgta gctcaagata
    67261 cggctgataa agtaaaaaca agtgcaagca aggaatccga agtgatcgtt acttctgcac
    67321 aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg
    67381 atgcagaaga aaaagcaaga aaaatcttga cagatgcaac agaaaaagca agacagcttg
    67441 cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga
    67501 tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat
    67561 tctccagcta cgtgactgac agccatgaag tgatcaaaga agttttagca aaacagcttg
    67621 acaatgaaaa tgatactgat gtaaactcag acacaggagt catagaagca cctatgacgg
    67681 catttgatac acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga
    67741 acagaaaagc actttgttat tcttaaagcg agccgatgag agtggaagtt cggtaagaca
    67801 ctgcatggtg aaagatcctc tacttgagac ctgcttttcc gaaattagag taagaagcag
    67861 cggctgacct cgttattggt cacagagagt ttgcactaga tagatgcaac taaatttggg
    67921 tggtaccacg gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa
    67981 ataaaggagt gtatggttga atgaaaatga aagaaacctt gcaattagga aaaacaaaat
    68041 tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa
    68101 atcgaatcta ccaaaaaaga caagagttga acgaaggaaa acctagcttt gttcttcatg
    68161 atggacctcc atatgccaat ggtaatatcc atttaggaca ttcattgaat aaaatcagta
    68221 aagacattat cattcgttca aaatcaatgt ctggctatcg tgcaccatat gttccaggat
    68281 gggatacaca tggattgccg attgaacaag tattggctaa taaaggcgtg aaacgtaaag
    68341 aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc caagtagata
    68401 agcaacgagc agatttcaaa cgcctaggag ttgcaggaga ttgggaacat ccttatgtta
    68461 cattagatcc ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaaa
    68521 gctatattta caaaggattg aaaccaattt actggtctcc gtcaagtgag tcttctttag
    68581 cggaagctga aattgaatat aaagatgtaa aatctccatc tatttttgta gctttcaatg
    68641 tcaaagacgg caaagatatt cttgatgaag atacagcttt tgtcatttgg acgacaacac
    68701 catggacatt gccagcaaac caaggtattg ctgtaaatcc aaaatttacg tatgtattgg
    68761 tggaagccga tggccgtaag tttgtcgtag cgaaagattt gctagaaaca gtccaatcag
    68821 aaatcggttg ggaaaatgta acagttctaa aagaatttgc tggacaagaa atggaatata
    68881 tgactgcaag tcatccattc tatgaacgtg aatcacttgt gattttaggc gatcatgtta
    68941 ctttagacgc tggtactgga ttagtccata ctgctccagg acatggggaa gatgactata
    69001 tcgctggtag caaatataaa ctatctgtag tgtcacctgt agactcaaaa ggattgttta
    69061 cagaagaagc acctggattt gaaggtatct tttatgacaa agcaaatcct atgatcactg
    69121 atctattaaa agaaaaagga gctttgctga agctagattt ctttactcat agttatcctc
    69181 acgattggcg gacgaaaaag cctgtgatct atcgggcaac tccacaatgg tttgcatcta
    69241 tcgataaatt ccgtcagaat attttggatg aagtagaaaa agtagagtgg gttatcccat
    69301 ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg cgattgggta atttcccgtc
    69361 agcgtgcatg gggagtacca cttccgattt tctatgctga aaatggagaa gcaatcatca
    69421 caccagaaac aatcgaacat gtggcaaaat tatttgctga acatggatca attatctggt
    69481 ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac ccacccaggt tctcctaatg
    69541 gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt tgattctggt tcttctcatg
    69601 aagctgtttt acgtcagcga ccagaattat cattcccagc agatatgtat ttagaaggat
    69661 cagaccaata tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct atcaatggtg
    69721 tagcaccata caaatcagtt atttctcaag gatttgcttt agatggtgaa ggccgtaaga
    69781 tgagtaaatc tttaggaaac atcattgcac cagaaaaagt gatcaaacaa tttggtgcag
    69841 atatccttcg cttatgggta tctagtgtag attatgaagc agatgttcgt gtttctatgg
    69901 atatcttaag tcaagtggca gaagtatatc gtaaaatcag aaatacgatg cgtttcttat
    69961 tagcgaatac agaagatttc gatcctaaaa aagatactgt ttcgtacaat gatttacgtt
    70021 ctgtagacaa atatatgacc gttcgattga accaagtaat caaagaaatc cgagaagaag
    70081 ggtacgacaa atataacttc atgcatattt accgtacagt tatgaacttc ttgacggttg
    70141 atctatcgtc attctacctg gattttgcta aagatgtagt ttatattgaa gcagaagatg
    70201 attatcaacg tcgttgcatg caaactgttt tctatcaaac agctgtagct ttgacaaaat
    70261 tattgactcc tatcattccg catacagcag aagaaatctg gtcattcctg aaagaagaag
    70321 aggagtacgt tcagttaagc gagtttcctg gttatgaaga gtttgcaaac caagaagaat
    70381 tgatggatac ctggactgct ttcatggact tccgtgataa tgtcttaaaa gcattggaag
    70441 aagcaagaaa cagcaaattg attggtaaat ctttggaagc aaaactaact gtttatccaa
    70501 aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa ttattgatcg
    70561 tttcaccaga ctactttgaa gttatggcag cagatgaaca agtgccagat aacgctatgg
    70621 tatttgatga tgttgctatt acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc
    70681 gccaagttcg taaagatgta ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg
    70741 caaaaatcgt tgaagacttc tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa
    70801 agggaaaagg gggtgtgaca tgttctttgt cacaccccct tttgttatc
//
LOCUS       DAZKSU010000005        66937 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000005 DAZKSU010000000
VERSION     DAZKSU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.005"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..1016
                     /gene="proB"
                     /locus_tag="ACG8Z3_000334"
     CDS             210..1016
                     /gene="proB"
                     /locus_tag="ACG8Z3_000334"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0822684.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1013..2260
                     /locus_tag="ACG8Z3_000335"
     CDS             1013..2260
                     /locus_tag="ACG8Z3_000335"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0822685.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2336..2680
                     /locus_tag="ACG8Z3_000336"
     CDS             2336..2680
                     /locus_tag="ACG8Z3_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822686.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2797..3123)
                     /locus_tag="ACG8Z3_000337"
     CDS             complement(2797..3123)
                     /locus_tag="ACG8Z3_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822687.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3496..4697)
                     /locus_tag="ACG8Z3_000338"
                     /pseudo
     CDS             complement(3496..4697)
                     /locus_tag="ACG8Z3_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4814..5329
                     /locus_tag="ACG8Z3_000339"
     CDS             4814..5329
                     /locus_tag="ACG8Z3_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0822688.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5528..5974)
                     /locus_tag="ACG8Z3_000340"
     CDS             complement(5528..5974)
                     /locus_tag="ACG8Z3_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0822689.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6021..7499)
                     /gene="msr(C)"
                     /locus_tag="ACG8Z3_000341"
     CDS             complement(6021..7499)
                     /gene="msr(C)"
                     /locus_tag="ACG8Z3_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0822690.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7853..8482)
                     /locus_tag="ACG8Z3_000342"
     CDS             complement(7853..8482)
                     /locus_tag="ACG8Z3_000342"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0822691.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8466..8930)
                     /locus_tag="ACG8Z3_000343"
     CDS             complement(8466..8930)
                     /locus_tag="ACG8Z3_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0822692.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9417..10685)
                     /locus_tag="ACG8Z3_000344"
     CDS             complement(9417..10685)
                     /locus_tag="ACG8Z3_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0822693.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10916..11653)
                     /locus_tag="ACG8Z3_000345"
     CDS             complement(10916..11653)
                     /locus_tag="ACG8Z3_000345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0822694.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11720..12289)
                     /locus_tag="ACG8Z3_000346"
     CDS             complement(11720..12289)
                     /locus_tag="ACG8Z3_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0822695.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12417..13289
                     /locus_tag="ACG8Z3_000347"
     CDS             12417..13289
                     /locus_tag="ACG8Z3_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0822696.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13631..14503
                     /locus_tag="ACG8Z3_000348"
     CDS             13631..14503
                     /locus_tag="ACG8Z3_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0822697.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14500..16458
                     /gene="nagE"
                     /locus_tag="ACG8Z3_000349"
     CDS             14500..16458
                     /gene="nagE"
                     /locus_tag="ACG8Z3_000349"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0822698.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16709..17560)
                     /locus_tag="ACG8Z3_000350"
     CDS             complement(16709..17560)
                     /locus_tag="ACG8Z3_000350"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0822699.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17683..18150
                     /locus_tag="ACG8Z3_000351"
     CDS             17683..18150
                     /locus_tag="ACG8Z3_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0822700.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18413..18895)
                     /locus_tag="ACG8Z3_000352"
     CDS             complement(18413..18895)
                     /locus_tag="ACG8Z3_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0822701.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18995..19858)
                     /locus_tag="ACG8Z3_000353"
     CDS             complement(18995..19858)
                     /locus_tag="ACG8Z3_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0822702.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20456..21304
                     /locus_tag="ACG8Z3_000354"
     CDS             20456..21304
                     /locus_tag="ACG8Z3_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0822703.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21475..21816)
                     /locus_tag="ACG8Z3_000355"
     CDS             complement(21475..21816)
                     /locus_tag="ACG8Z3_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFQ0822704.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21902..22609
                     /locus_tag="ACG8Z3_000356"
     CDS             21902..22609
                     /locus_tag="ACG8Z3_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0822705.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22596..22919
                     /locus_tag="ACG8Z3_000357"
     CDS             22596..22919
                     /locus_tag="ACG8Z3_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0822706.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23008..24045)
                     /locus_tag="ACG8Z3_000358"
     CDS             complement(23008..24045)
                     /locus_tag="ACG8Z3_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0822707.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24492..25475)
                     /locus_tag="ACG8Z3_000359"
     CDS             complement(24492..25475)
                     /locus_tag="ACG8Z3_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0822708.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25891..26661)
                     /locus_tag="ACG8Z3_000360"
     CDS             complement(25891..26661)
                     /locus_tag="ACG8Z3_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822709.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26654..27589)
                     /locus_tag="ACG8Z3_000361"
     CDS             complement(26654..27589)
                     /locus_tag="ACG8Z3_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822710.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28183..29832)
                     /locus_tag="ACG8Z3_000362"
     CDS             complement(28183..29832)
                     /locus_tag="ACG8Z3_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0822711.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30027..30923)
                     /locus_tag="ACG8Z3_000363"
     CDS             complement(30027..30923)
                     /locus_tag="ACG8Z3_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0822712.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30916..31596)
                     /locus_tag="ACG8Z3_000364"
     CDS             complement(30916..31596)
                     /locus_tag="ACG8Z3_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0822713.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31597..32379)
                     /locus_tag="ACG8Z3_000365"
     CDS             complement(31597..32379)
                     /locus_tag="ACG8Z3_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0822714.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32372..33289)
                     /locus_tag="ACG8Z3_000366"
     CDS             complement(32372..33289)
                     /locus_tag="ACG8Z3_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0822715.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33292..33576)
                     /locus_tag="ACG8Z3_000367"
     CDS             complement(33292..33576)
                     /locus_tag="ACG8Z3_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822716.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(34036..34428)
                     /gene="rpsI"
                     /locus_tag="ACG8Z3_000368"
     CDS             complement(34036..34428)
                     /gene="rpsI"
                     /locus_tag="ACG8Z3_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0822717.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(34444..34872)
                     /gene="rplM"
                     /locus_tag="ACG8Z3_000369"
     CDS             complement(34444..34872)
                     /gene="rplM"
                     /locus_tag="ACG8Z3_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0822718.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(35406..35873)
                     /locus_tag="ACG8Z3_000370"
     CDS             complement(35406..35873)
                     /locus_tag="ACG8Z3_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0822719.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(35993..37531)
                     /locus_tag="ACG8Z3_000371"
     CDS             complement(35993..37531)
                     /locus_tag="ACG8Z3_000371"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0822720.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            37611..38297
                     /locus_tag="ACG8Z3_000372"
     CDS             37611..38297
                     /locus_tag="ACG8Z3_000372"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0822721.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(38498..39025)
                     /gene="msrA"
                     /locus_tag="ACG8Z3_000373"
     CDS             complement(38498..39025)
                     /gene="msrA"
                     /locus_tag="ACG8Z3_000373"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0822722.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(39109..39786)
                     /gene="pgmB"
                     /locus_tag="ACG8Z3_000374"
     CDS             complement(39109..39786)
                     /gene="pgmB"
                     /locus_tag="ACG8Z3_000374"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0822723.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(39783..40913)
                     /locus_tag="ACG8Z3_000375"
     CDS             complement(39783..40913)
                     /locus_tag="ACG8Z3_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0822724.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(40913..43096)
                     /locus_tag="ACG8Z3_000376"
     CDS             complement(40913..43096)
                     /locus_tag="ACG8Z3_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0822725.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(43093..44118)
                     /locus_tag="ACG8Z3_000377"
     CDS             complement(43093..44118)
                     /locus_tag="ACG8Z3_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0822726.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(44163..44990)
                     /locus_tag="ACG8Z3_000378"
     CDS             complement(44163..44990)
                     /locus_tag="ACG8Z3_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0822727.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(44987..46054)
                     /locus_tag="ACG8Z3_000379"
     CDS             complement(44987..46054)
                     /locus_tag="ACG8Z3_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0822728.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(46080..46916)
                     /locus_tag="ACG8Z3_000380"
     CDS             complement(46080..46916)
                     /locus_tag="ACG8Z3_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0822729.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(46913..47782)
                     /locus_tag="ACG8Z3_000381"
     CDS             complement(46913..47782)
                     /locus_tag="ACG8Z3_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0822730.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(47852..49135)
                     /locus_tag="ACG8Z3_000382"
     CDS             complement(47852..49135)
                     /locus_tag="ACG8Z3_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0822731.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(49149..50834)
                     /locus_tag="ACG8Z3_000383"
     CDS             complement(49149..50834)
                     /locus_tag="ACG8Z3_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0822732.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            51109..52134
                     /locus_tag="ACG8Z3_000384"
     CDS             51109..52134
                     /locus_tag="ACG8Z3_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0822733.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(52729..56382)
                     /gene="rpoC"
                     /locus_tag="ACG8Z3_000385"
     CDS             complement(52729..56382)
                     /gene="rpoC"
                     /locus_tag="ACG8Z3_000385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0822734.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(56461..60078)
                     /gene="rpoB"
                     /locus_tag="ACG8Z3_000386"
     CDS             complement(56461..60078)
                     /gene="rpoB"
                     /locus_tag="ACG8Z3_000386"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0822735.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFVDQTNPLGELTYKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            60806..61774
                     /locus_tag="ACG8Z3_000387"
     CDS             60806..61774
                     /locus_tag="ACG8Z3_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0822736.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            61792..62697
                     /locus_tag="ACG8Z3_000388"
     CDS             61792..62697
                     /locus_tag="ACG8Z3_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822737.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            62694..63500
                     /locus_tag="ACG8Z3_000389"
     CDS             62694..63500
                     /locus_tag="ACG8Z3_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822738.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            63657..64625
                     /locus_tag="ACG8Z3_000390"
     CDS             63657..64625
                     /locus_tag="ACG8Z3_000390"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0822739.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(64942..65466)
                     /locus_tag="ACG8Z3_000391"
     CDS             complement(64942..65466)
                     /locus_tag="ACG8Z3_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0822740.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            65753..>66937
                     /gene="scm"
                     /locus_tag="ACG8Z3_000392"
     CDS             65753..>66937
                     /gene="scm"
                     /locus_tag="ACG8Z3_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0822741.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SAS"
BASE COUNT        19394 a        14239 c        12286 g        21018 t
ORIGIN      
        1 actactccta ttctaacata tccgaggctg taaaagactt tctttacaaa ttcttcaaga
       61 aattctgcta tcttaaagta aaccattaat tcgttctttt tccaaacgac tgcaagttgt
      121 acaaaaaaac tattttttac atgactttac tcgtcaaaac aagtagaata gaacgaaatt
      181 aatgagtgga tatttgattg aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa
      241 ttattgtcaa agtaggaaca agtacattga tttatcccaa tggaaatatc aatcttgcgg
      301 caatcgatca gttagctttt gtcctttctg atcttagaaa tcggggaaag gaagtgatct
      361 tagtctcatc aggagctgta ggcgttggta tgcataaatt acatttgtca gaacgtccga
      421 ctttgattcc tgaacagcaa gctgcggcgg ctgttggtca agccgaactg atgaatatct
      481 ataatcaaag attcagtgta tataatcaac agacggcaca gatcctcgtg acaagagacg
      541 tggtagatta tcctgaaagt cgtaaaaatg tgaggaatac attggaacag cttttgatta
      601 tggacacgat ccctatcgtg aacgaaaacg atactgtagc agtggatgaa ttagatcatc
      661 tgacaaaatt tggcgataat gatcagctgt cagccatcat ggcaaaactt gtcgcggcag
      721 accttttgat cgtcctttct gatatcgacg ggttttattc agataatccg actacaaatc
      781 ccgaggctca gatgtatcgc tatatcgata aaatcgatga aaagattctg gcacaagcag
      841 gtggtgctgg tagcacattt ggaacaggcg gtatgcagag taaattaaaa gccgcttcta
      901 ggatatttga agcaaacagc gcaatgattt tagcaaatgg aaaaaatccg gcaattattt
      961 ttgatattct tgcaggaaaa gaagtaggta ctttatttaa ggaggacttg aaatgactga
     1021 cttgatgcaa ttaggacaac aggcaaaaga tagcgcgcaa caactagcac ttatgcctac
     1081 aaaccgcaaa aatgagctac ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc
     1141 gatcattgaa gcaaatcata aagacttaga aaaagcagtc gaaaacaata tcagtgaaac
     1201 gatgcaagac cgcttgcagc tgacacctga gcgaatcaat gccatggcta cagagatcga
     1261 aaaaattgct tcgttagacg atcctattgg aaaagtagat gagatgtgga cgaacaccga
     1321 cggattgaga atcggcaaaa agcgcgtgcc actaggcgtg accggcatca tttacgaatc
     1381 tcgtccaaat gtcacgacag atgcagctag tctagcattc aaatcaggga atgccgttat
     1441 ccttcgcggt ggcaaagaag cattcttctc gaatcaatta ttagtccaat tattgcagca
     1501 agtactggtc agtgctggcg aatctcctta tgccatccag tttgtcgatg atacttctca
     1561 tgaaactgca ggacagttga tgcgattgac agaatactta gatgtgctga ttcctcgagg
     1621 cggagcaaaa ttaatccaac gagtcaaaga acaggcaacg gtacctatca ttgaaacagg
     1681 tacaggaaat aatcacgtat atatcgataa agaagcacag ttatctatgg cagtgaacat
     1741 catcgtcaat gcaaaagctt cccgtccatc tgtctgtaac gcggcagaaa cattgctgat
     1801 ccattctgag atcgcacctt ttttcttgcc agctattgaa aaagaactgg tagaacacgg
     1861 ggtctcttta cgtgcggatg ctcgtgcgtt agaatatcta gagacggctg ctttagcaga
     1921 agacacagat tgggatactg aatatctaga ttatatcctt gctgtcaaag tggtcgattc
     1981 tttggatgaa gcaattgcac atatcaatcg gcataatact aaacattcag aaacgatcgt
     2041 gacagatagt tatgcggcta gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta
     2101 tgtcaatgcc tccactcgtt ttacagacgg atcggtattt ggttttggtg cagagattgg
     2161 tatctctact caaaagttgc atgcccgtgg tccaatgggg ttaaatgaat taacttccac
     2221 aaaatatatc atttatggcg atggacaaat ccgcgaataa tgtttcgtgt gaaacattat
     2281 tcccgccttt tgcctttttt ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt
     2341 tgaaatttta atgatttttt ttatttacct tatttctctg aatatcgctg cttttctggg
     2401 agtcggtatt ttatcccttt tctttcaatt caaaaaacga agcataggga gtcaacggga
     2461 aaaatggtcg cagtattttg acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat
     2521 tagttacatg gttgcattat gcttattagc gacaatcaat tactattctt ttttcgatca
     2581 ttcaatcgct tatacgatta cgctactgat tgctgggatt tttcacttat cttataaata
     2641 tcagctgaat aaaaaccact tgaatcggac tttccgctaa aaaatatccg tacgataagt
     2701 agagtgcctt gaagatcggc aaaaaactgc cggacttttt agaggctgtg acaaagattt
     2761 tgtcacagcc tctttttcca tataaacggc gttcgcctac cagctgcctg gtaaatctgt
     2821 ttcctcttcg ttaagaagcg aaaatccgtc aaatgtttcg ccatcataat aacgaagcag
     2881 ctcatccatg gacaagttca tcgttttttc tttttcttct tccgctgatt cagccagtag
     2941 ctgttcccga tacacctgat acataccagc tattttttgc tgcatcgctt ttctagtttc
     3001 accttccgca ttgattattg gcaattcttt gatatagcca cgataaatca accgatcatc
     3061 tgaaaattca atgggtaatt caacgagtgt caacccgact tcctgaaaat attcttccag
     3121 catctattat ccccccattc tttttcttaa atgaatttta gcttacctac tgaaaatttt
     3181 ccagtttcat tattttgaga aaaaaaggga atgtgacaaa aatcgtgtca catccccttt
     3241 ttccgaataa atggtgttct tctgccaact cctcaatcat aagtcacaat attcaacaaa
     3301 catgagaaac tgttttttga aattgttcct tgtgattcag agttaaacga cagtctcaca
     3361 acctcttggt aaacggcgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3421 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3481 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3541 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3601 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3661 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3721 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     3781 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     3841 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     3901 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     3961 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4021 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4081 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4141 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4201 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4261 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4321 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4381 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttc
     4441 aaaccgatcc gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg
     4501 taactagtaa ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt
     4561 tttaagattg ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca
     4621 actagcgctg aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga
     4681 ttctccaata tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt
     4741 aaatatgcac atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag
     4801 aggtaggatt tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc
     4861 acgttctggg tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca
     4921 gatctataca acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga
     4981 cgagcactct caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga
     5041 atggatcaaa gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta
     5101 tgctttttcg acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca
     5161 gctgaacaaa atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa
     5221 acaagaattt ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact
     5281 ccaatggtgt cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag
     5341 aacctttcta gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt
     5401 ggaaaagaaa aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc
     5461 tagaactgca ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag
     5521 tgttccatca ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga
     5581 tgatcaactg caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag
     5641 agaataattg atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca
     5701 ccattcctaa aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat
     5761 ttactggtag tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa
     5821 tgaaagccac cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt
     5881 gatgcagcca taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg
     5941 taaaaattat tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca
     6001 cgaaaatcat cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct
     6061 tttatttcat aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac
     6121 aaaatcgttc ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca
     6181 ataaaattag tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca
     6241 atcgtcagac gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc
     6301 tgagcaaacc ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt
     6361 ctcatgacaa aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat
     6421 acttgaaagc ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct
     6481 aataaggttg tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa
     6541 aactgaaaat caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt
     6601 cccatgatgg gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt
     6661 ttcatctgct gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc
     6721 gcattctttt ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc
     6781 ggtctgattt gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt
     6841 ttcttttctt ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct
     6901 cgtagctgag tctttttctc caattccttt tgagtgagat aggcactata attccccgaa
     6961 aactctcgga tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa
     7021 tagcgatcat gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt
     7081 cgatcaatac tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca
     7141 taagaagaaa gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc
     7201 tctgaagttt cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt
     7261 gcttcagcga gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc
     7321 ccatgatcgg gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca
     7381 ttttctccta tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt
     7441 tctagctggt tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata
     7501 tgttttacac tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa
     7561 atctgtttgc aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact
     7621 aaatcaccaa atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata
     7681 ctccatccat gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa
     7741 agttcgaaac gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta
     7801 ccacacacga ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga
     7861 acgtagtgat cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc
     7921 ctattgataa aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca
     7981 actgatctgc ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta
     8041 acatgaattg catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc
     8101 ttgaaaaact cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg
     8161 gcatcttccc tgattcgtaa gcttggttat agatttcttc gccatattcg taacattgca
     8221 tatcgccaaa aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga
     8281 tcaaaggacg caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt
     8341 gcatattgac agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt
     8401 gtgggatttt gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg
     8461 aaaaatcatt tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa
     8521 agttgggtat tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa
     8581 gccggggata gcacggaacc tgtatcttta tggtccattt cacaatgctc gattttcttc
     8641 tgggcttttt ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc
     8701 gaaacatagc catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc
     8761 tgcaactttt cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg
     8821 atctcgtatc tagtaagact tataccgata ttttcttcaa agatctggac gattttttga
     8881 tcggctaatt tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc
     8941 tttctaataa ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa
     9001 gtcaaagaat cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga
     9061 aattttttgt agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt
     9121 attgattctt atcaaactat agaagatagc tcttctgcag tagaaaattt tacaccgcca
     9181 aaaatttccg aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat
     9241 gcttaaacag taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg
     9301 gtgttcaaaa acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg
     9361 tttttgtttg gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa
     9421 taaccataaa atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc
     9481 ttagcactca aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa
     9541 attggcttgg aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt
     9601 aaagagttga tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat
     9661 aaacggtaga caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag
     9721 cctacagttg gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta
     9781 tgacggttat acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc
     9841 agattatagg tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa
     9901 cgacctgtta gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg
     9961 gaatagtagt aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc
    10021 aatgattctt tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca
    10081 ttcacatatt ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca
    10141 aatagattgt aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg
    10201 gcttgagcaa tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt
    10261 gaggcaatac tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca
    10321 tagccagtat agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc
    10381 ccatctgtca attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct
    10441 gatgtactgt cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa
    10501 attgtatttt ctgagacagt agctgactgc aaagtactct ctgtatcgtt ttcaattcct
    10561 gtttctgaag aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact
    10621 gttgtgttgg caactgctag atttcctaaa agaataacgc taaataataa attctttgct
    10681 ttcatctttt ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa
    10741 gtaaaagcaa gtcaatgtcc atatttaaat aacatttgtt tctttttgat aaatttgctt
    10801 tttctacata aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg
    10861 gtcgtttgaa taactattta acaagtaatt ctctttggat attatccgtt atggattata
    10921 cttcctgtgt cgtcggacgg ataatcatgt tactgacaga catccggtct ggcgtttcta
    10981 tcgcaaatgc tacagcagaa gctatatctt cggaggtcaa tccccattct ttttgcgctt
    11041 catgaagttc aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag
    11101 ctcctggaga gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt
    11161 ccatgattgc acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa
    11221 catgtcccgc cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg
    11281 gaagtacggc ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc
    11341 attcatcacg gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca
    11401 atacatcgat tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa
    11461 aatctcttac atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg
    11521 cttttaggcg atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata
    11581 gcttcgttgt tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg
    11641 ataaatcatt cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc
    11701 tcttatttca tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga
    11761 ttcctgcaac gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa
    11821 tggcattctc gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat
    11881 ttttcttgat gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat
    11941 ggttcaccgt gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc
    12001 cacctaagtc aacgaacatc acttgacgat tgataaagac atttttccct atactaatat
    12061 gtttgccgaa atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac
    12121 taatgatatt ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg
    12181 ctgctaaagg ctcttcgttt tcagcttgga tctgatggat cacttcaaag agcggatccg
    12241 ttactcgaat ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc
    12301 cttttatcct tactttataa cggacggctt atcatgtcta atacttataa aacatactta
    12361 actattcctt ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg
    12421 atattcgtat actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag
    12481 caaaatctct gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag
    12541 aacttggtgt gacacttttc actcggggct cccgagagat acagttgacg gattctggtc
    12601 gatatcttta ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt
    12661 taacaaatga cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa
    12721 tctctttcat ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc
    12781 atttatatag cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt
    12841 ttggactcgt aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc
    12901 aagatcgttg gggacttttg atacatcgaa atcatccttt agcaaagcaa accgtcatcc
    12961 atccgcagga actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc
    13021 aactagctga atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact
    13081 tattgtacaa tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg
    13141 ggatcatcaa tacttatggg acggaaaaaa tattcatccc actcgatccg ccattacaag
    13201 cttcactgaa tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt
    13261 tagatgtcct aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt
    13321 taacaacgaa taagataagc aaaagatcca acgattaaat aacataagtt tcttaattta
    13381 tttcttcctt ttatttctga taattccagt aaattactat aattataaaa aaagaattga
    13441 caaaacataa ctgtctatat aaaattgatt acgttaacaa taattcgttt catgtgacta
    13501 gatcatacta ggcatgaaaa atctctgagg aacatggttt ccctaggagg tttttcgtgt
    13561 ctatttttgt ttggccgcaa tcaactggtc tagcaaacgt gaaaaccaaa tcttcaaaaa
    13621 gggtgcgcgc atgaaaatca aaaagatctt taatcaaaat gcagtattgg tagatgacgg
    13681 tggacaggaa aaagtagcta ttggaaaagg gattggattt gacagaaaaa gaaatgattt
    13741 gatttttgat cgtgatgtcg aacggatatt tgtcatggaa ccagaaggtc aaatcaagtt
    13801 gcaaaattta cttaatcaga ttgatgaacg ctttttattt gcagcagaaa cgatcattga
    13861 tcatgcggaa actgttttga tggaaaagtt gaatgaacat gtattgatcg ctttgaccga
    13921 ccacattgct ttttctgcgg agaacattaa taacggtatc gtcatccgca acaaactttt
    13981 aagagaaatc gaagtgcttt acagtgatga attcagtatt gctcaatggg cagtagagta
    14041 tctgacaaaa gagcttggga ttccatatac ttatgacgaa gcaggatata tcgcaatcca
    14101 tattcacagc ggacgaagcg gtcggaacaa taaccatcgc agtatccgtg aagtgacgat
    14161 tgtatctgac atcatccatt tagtggaatc agaattggaa acagatatcc attcagaacc
    14221 attttcactg aattattcaa gactggtgaa tcatttacga ctgcttctac aacgaaccca
    14281 tgcgcaacaa tatgctgttt tagacacgga aatcgtagaa atggtcaaac gaaaataccc
    14341 agaaagctat aagatttcta aaaaaatccg tgtgttgctg accaaagaat accagctcgc
    14401 gatcacgaaa gaagaactgg gttacttggc catccatatc gagcgtttaa gaagcgcaat
    14461 cgtacaaaca aatgttaaca atcatgagga ggaaaaaaat tgaaagcata tctacaacga
    14521 atgggacgtt cccttcaact tccagttgcg gtcttacctg ctgctgcttt actagtaggg
    14581 atcggaaact ggtgggcatc ttatagtaac gacattgtcg ctcatttttt acaagctggc
    14641 ggtaacgcag ttttaggtca attacctttg ctgtttgccg ttgggttagc actagggatg
    14701 tcagtggaca aatcaggtgc tgctgccttg gcaggtttgg tcgcatttga agttccagta
    14761 aatgttttaa aaactgattc agtagctact ttattgaata tcaaaccaga atcagttgat
    14821 ccatcttttg ctcagatcag taacgtattt attggtatta ttgccggatt gattgcggca
    14881 gctctttata atcgtttcca cgaagcaaaa ttaccgatgg ctttatcttt ctttagcggc
    14941 aagcgtctag tgccaatctt ggctgccttt gtcatgttgc tgatcagtgc agtgctactg
    15001 ctcgtatggc ctccggttta ctctgcttta gtttcttttg ctaaattcat tttgagttta
    15061 ggctggggtg gtgcaggtct ttatggcttc ttcaaccgtt tactgatccc tacaggattg
    15121 catcaagcaa tgaactccgt gttctggttt gacgtagcag gaatcaatga tattggtaaa
    15181 tttttggctt ctgaaggaac aaaaggcgta acaggaatgt atcaagctgg atttttccca
    15241 atcatgatgt tcgggcttcc agcaggagca tatgctattt acagaaatgc acgtccagaa
    15301 aacaaagcaa aaacagcttc cttgatgcta gcagcagctt ttgcttcatt ctttactggt
    15361 gtgacagaac cacttgaatt ttcatttatg ttcgttgctt ggccattata tgtcttgcat
    15421 gcaattttca ctgggatttc tttagcagtg agtgcattct tccattggac agcaggattt
    15481 gcctttagtg ccggatttgt cgattacttc ctatctttga aaaatccgat tgcgaatcag
    15541 ccattgatgt tgatcgtcca aggattagta acagcagtga tttactactt tggctttaac
    15601 tttgctatca aaaaattcca tctgatgact cctggccgcg aggaagcaga tctttcagat
    15661 gacgatactg caacgactaa ttcagacaat aaatatgcgg cacaagcaag caaaatctac
    15721 gctgcattag gcggagccga caatgtaact tcgatcgata actgtactac acgccttcgt
    15781 ttgcaagtca aagatacagg cctgatcgat caaaataaaa tcaaagccac tggcgtacct
    15841 ggtatgaaga tcatcgacgg aaaaaatgcg caagtcatcg taggtacaga agtacaattc
    15901 gttgcagatg agatggcaaa actacatggt ggtgctgcag cacgacctgc acaaacaaac
    15961 accgtggtta aaacagaaac atttgccggt gaaacgatcg aacaagatat ttacgctatc
    16021 gcaaatggga aactgattcc aatcactgaa gtttcagatg atgtattcgc ggaaaaaatg
    16081 atgggtgacg gctatgctgt attaccagaa aatggagaaa tcttctctcc aatcgctggt
    16141 acgatcacaa acattttccc aacgaaacat gctgtcggaa tccaaacaga tgctggtatc
    16201 gaagtcttgc ttcatatggg catcaacact gttgacttaa aaggcgaacc attcacttta
    16261 tatgtagaag aaggtcaaaa agtcgcaaga ggtcaattga ttgctttagt tgatcttgcc
    16321 gctattcaat ctgcaggcaa aaacacggat atggtcgttg tattcacaaa tggcgacaaa
    16381 gtccaaagct tagaaatcga acccgcacgt gatgtaaaag caaatgataa aatcggctct
    16441 gtttccaaca aagcctgagc catcgtcttc cccagacttt tttatcgata aacaaacagg
    16501 agctgagaca aaaatcttgt ctcagctcct ttttccgaat aaaaacggcg ttctcctgcc
    16561 aactccgcgg taataagtcc agctaaacga aacatatgaa gagctatttt ttatttgact
    16621 cttcttatta cttgatgcag aacagctttt tcacaaaatt tttcttcata ctcaaaacta
    16681 atcggctttt tcacaacctc tttatacatt attcctgatc caatatttct tttaatgctg
    16741 cttcaagaga agttaatggt tttcccaata ctttttctaa atcatcagag gaaatagcta
    16801 actgatcctt tttaatatca tgttggatcg ctaaaaacat gtcagctgct tcttgaggga
    16861 atccggcttc ttttagattt tcgacaaacg ctgcatcact tgcttcaata atttctaact
    16921 ctttacctgt cgcacgctct aatgccttgg ctaattcttc atagtcagtt agattgccgg
    16981 ataattctaa gatttctgga gagtcacttt ctactagtac ttttgctgca gcttctgcat
    17041 attcacgttt caatgcccaa cctgtttttc catttcctgc agcataaacg aattgaccag
    17101 tttttaaagc ctgcccaatg ataggtaatt cattctctac ataccaatta ttccgtaaaa
    17161 atgtatggga aatacctgaa tccttaatca ttttttcagt gaattggtga tctggcgcta
    17221 atacactgat cgagtgatcg gcatctgcaa aacttgtata agcgatataa gataccccag
    17281 ctgcttgtgc tgccttcact acattcttat gttcggcttg acgatttcct ggaacgccag
    17341 agacaaaaag caatcgatct attcctttca acgcttcttt cattgagtct aaatcatcat
    17401 aatcaccaat acgtatatta aatccagctt ctttcagtgc ttctcctttt tcttcactac
    17461 gtactagtac atagatttca ttttgtggaa ccatcttttt taagaattcc agcgcataac
    17521 caccaaaacc tccagttgct cctgttacta aataatgcat atttgccact ccctattctt
    17581 tttatttgtt aagatttaca gctgtacatt tataaattac acctttatca taaagagtgt
    17641 ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac tgatgaagta ttctctgcaa
    17701 tttagtgatg caatccatgt acttgcctat attgaaatgt ttcaagatac ccgatcactt
    17761 tctagtgaga tgattgccag tagtatcgaa acaaatgcag caaatgtacg taaaatcatg
    17821 agtcagctca aaaaatccgg tttgatcttt acaaaaaccg gaaaaccgca gccttccctt
    17881 gccaaaagtc cagatcagat ttctttacta gatgtctatc gcagcatcga aggtaataca
    17941 cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg tcgttggtgc gaatatccaa
    18001 gaagctttag aagaaagcta cgctcggctg cagaaaaagg cagaagaaga aatggctaag
    18061 atcacacttg atagtctgat acataaaata gccgttttgg aaaaagaaaa aagaccagaa
    18121 aacacaggcc tcgtagagag atttttataa ttctctctac tgtgcctgca tttttctgat
    18181 cggaatcgtc tacataaatt cttttattct ccgggggaca aaagccttgt catgcccctt
    18241 tttcctctct aatgctcagg tcaaagcgct gaagtcatga cgtcttgata aacggtgttc
    18301 aagtagctga ccctcggcat tagttctgaa aaatgcaaaa tatggaaagc tatttcccat
    18361 ttttcctctc taatgctcag gtcaaagcgc tactttcacg acctctatat tatcatggtt
    18421 tattgactac cggtatccag atttctccca tatatttccc gttggtaact cccatttcga
    18481 ctacagcatt tggaccccca acataggcgt aatcttctac ttctcttaaa acatgcccaa
    18541 aggccgtatt cgtcaaatca tttcttaatt cttcaggcga ttctgctaaa gcgcttacaa
    18601 caagatacgt tccttttggg aatgggatga ttcgcgtagt gtttggaaca tcctgactcg
    18661 tttgaacacc gacatagtac atcattttcc cgttataggg ttcgttgact gtaaagaaat
    18721 aatgattttc agcagcttgt ttcaatgcat ctattgttcc attaccgata actttttgct
    18781 caaatgcttg tttctctgca gccaaaccga caaaatccgt attgctactc ttcaattcca
    18841 caccaatacc cgtcaccgta aaatctggtt tttcttcgat cctatacttt gccatttctc
    18901 ccaccctttc tattatatta agttcatctt acataaaatt aaagggaaat gacacgatta
    18961 cgctcttttt tctagggata tttttatttt ttaactattt ttttgctttc actgcaatcc
    19021 aaatctcact gtaaacatta ctgaaatcct ctccccattt tgtaaaagag atctcgggag
    19081 cttcgactaa cccataatct gaagaaggaa gccattctgc atagattttc ccccaagtat
    19141 cttgtaaagt ttggggaaat ggccctttat taggaaaaat ggcccaagtg tgttcttcta
    19201 tagcaagcat ttctaaatca gcgtacggat tttcttttgt cgtagcaaag ccaatcatgt
    19261 gcatcaaact gcccttttct tccaagcggt ctccatcaaa tccataagaa acgttgatta
    19321 cttgatttgg atataaatct cctaacttat gcatctcttc cctttgagtc tccgtgatcg
    19381 tctgcgctaa cttttgtatt tcttgattga ctccttcaaa tttgacagga acttttttcg
    19441 ccacacctgc cacataaaac tgttctttct tttcgatacg aaattccatc gattttcctc
    19501 ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt ttgtatgccg tatctactga
    19561 tttctgaagg cagatatcct gaccaatcac gaaaagctct tgaaaaacct tcgactgttt
    19621 catatccata tctaaacgcc gtttctgtca ctcttgatcc atctagaaga tcgcgattag
    19681 ccaaagccaa tctacggttt ttgatatagt ccattagcga catccctgca ataaacgtaa
    19741 agatgcgttt taattgatat tcagagattc ctgtattttc ggctattatt ttataggaaa
    19801 gtccttctgt caaatgttct tcgatgtaat cgatcaattg gttcagatct ttaagcatat
    19861 ttatcttcct ccttgattgc ctttttatca tattatcaaa gaaagtcaat tacttgatga
    19921 atcaggaacc aatttagtca gatgtagaaa atgttgtatg gatctaaaga aaattctcaa
    19981 gcagcgtttc gtaacggata attggtatta tctgcctatc tatgttttac ctttagaaaa
    20041 ataaattttg gggaagacag attctaagta aacaaagcgg taaaaatatc ttttattccc
    20101 ttagttaatc cgcttactac ctactgtaca aagcaaattg ttgttcaaac tgctgatcag
    20161 aacgctgctt ggatttttct ttgcgataca tcatacctct tttatcaatt ggaacaatta
    20221 tttatttcta acaaaatcac ttcatttttg tcagataaat aaaaaacaaa aaaagttcca
    20281 tctttaaaac acgctattct tttacttttt ctcacattct ctaagaaaag aaaaggagaa
    20341 tgaatttttg acaattcgag caaaagtgat tatcaaatat gtaaaatcag ctactttttt
    20401 cttaaacgtt tctttatact aaatataaat aagtgtttca tgaaaggaga actagatgaa
    20461 taattggctt gagtattttc ctgaaaacgt tttggaacgc ggttacagct atcatctgca
    20521 cggttttgtc cgtcatttga actatacaag caagtacttg tccgcaacag tttctggcac
    20581 ggaagattat aaggtagtga tcacttggga tgaaaagacc aacatgactt gtgattgtct
    20641 atatgccata gaaggaaaaa aatgcaaaca catggctgct gtcttattcg cttacgaaga
    20701 acgtccaata aaaaaatcca actactcact ttccgaactc tcgtcccttg tttcctccgc
    20761 tagttcctct cttgtacggg agcttttaac agagattctt atagaacatc cgcactttat
    20821 cgaacgcttc aaagttaaga tgccatttca tgcaataaat tattcagata aattaacaac
    20881 tatcatccat aaatacgatc atattatcaa gaaaaacaaa aacaggaaaa cagcgaagtt
    20941 catcatggag atgcgaaagt tcatccaaga agcagttgaa tcgcttattc agcaaaatgc
    21001 ctatcttccc gcattcgaat tgatcaatga agtgattgct actctcgaaa cattctattg
    21061 ggagcctgag gatgaacgaa cccttcttct gatagaggac tgttattatt tatggaaaga
    21121 attattagca gaagcacctc atgcagaaaa aaggcaaatg ttctcttggt tcgtctgcca
    21181 agtcgatcat actgacgcct cttattcaaa acgctactct ataaaaatcc tgaaagaaga
    21241 ctttcgggaa aaagaatttt ctaaccaaaa gaaaaaaata gataaaaaac taaagaaacg
    21301 gtaaagaaag acgatttcaa tgaaaaatag caagtaaacg ataacaacca tcctaaatcc
    21361 acaagaaata cagcagacct tgctttaaaa acacgaatca tgcaaaggat gaagtaaaga
    21421 agacttaacg cgtacccaat attagtcatt tgactattag gtacgcgttt tgttttataa
    21481 gtcatgacaa atttccttcg ttgctggata gattttccgg cattgttgat agattttatg
    21541 atacttttct acattttgta agagaggttc cgctgcatct gtatattgga taaaattctg
    21601 acaacaactt tcaggtgtgt cgaaccaccc taaccctagt gcagcaatca ttgttgcgcc
    21661 taatgcaggt ccttcctcca cacttaaaca atagaccgtt gcatcgaaaa tatcagcttg
    21721 gatttgtagc catgcaagat ttttcgcgcc tcctcccacc gaaacaatac gtgtaaatct
    21781 gcgacccgct agttctttac gtttcaatcc caccattgac aaatgctcat taaaaattta
    21841 gaatattcac aagtttaacg acaattatga aaatttatgt cgtgctaaag gagttttgtt
    21901 attggatgaa aagttagata taaaaactgc gatcaaagac acgttaccta ccgttttcgg
    21961 ttatatcggt attggacttg catttggtat cgttgggaaa gctgccggat ttcatccact
    22021 agtcgtcacg ttgatgtccc tgctggtcta tgctggttct gcccaattta tcacagtcag
    22081 catgcttgct agtcacagcc cattgctttc catcgttttc tcgacctttc tagtcaattc
    22141 ccgaatgatt ctgatgagta tgacgattgc tccttatttc aagaaaaatc ggctgcttca
    22201 aaatctgctg attggcacat tgctgacaga tgaaagtttc gcactgggaa tgaataaatt
    22261 gaactatact ggacaaaagt taaactttcg ttggatgaat acagctaatt ggatttctta
    22321 tttgacgtgg gtgggttctt ctcttgttgg cgcactttta gggaatttta ttactgatcc
    22381 gaaaaagttt ggtttggatt tcgccatcgt cgctatgttt atcgggttac tttatcttca
    22441 agtcatttct gatagaaaca cctctaaacg attacagttg atcctgattg gtctcacttt
    22501 gattctggta tatgtcggac tgatttttat cccaagcaac ttagtgattg tagttgtaac
    22561 actgattggg tgcggattgg gggtgtggat caaacatgcc ttcttttgag tttacattgc
    22621 tgactatttt aggttgtact ttagcaactt ggctttctag ggtcctacca tttgtcttac
    22681 tgaaaaaatt cgatttaccg caaccgttgc ttgagtattt aagttttgtc ccgatcgtca
    22741 ttatgtctgc tttatggttc agcagtctat ttacccaaaa tatcggacat ctgccgcaga
    22801 tcaatctcga aaatgctttg gcttcagttc ctacactttt agctgctatt ctttcaaaaa
    22861 gcttattggt gatcgtcttg gcaggaattc tttccctttc gttgattcgc ttattataaa
    22921 taaaaaaaca agagcttatg gcaaattact gtcatgcgct cttgttttta tcaaagtcaa
    22981 ataagaaaaa aatcggcgta ttcttgctta tccgttgaac ggaccactaa caacgacttt
    23041 tcctaacata tgtcctgttt ctacgtcttt ggttgcttgt ttcaagttct tcgcattcat
    23101 cccatctgaa tagaccttgc tcaatgtact gcggataatc ccttcatttg ctaaagcagc
    23161 aatgtgcgct aaagcttctc cttgcgtttc gatttcatag ttatagtctg ttttagcgaa
    23221 catatactcc cagtcgaagc tgatagattg gtttttccaa acacctatat ctaacggctc
    23281 tttgatccct acgatcgttc ccacatgtcc aaatggtcgg acaaggtgct tgatttgatc
    23341 aaagtagcgg gtaatatcga ataatacagc gacatactct acttgatcga ttcctaattc
    23401 acgaaaactt ttacttaagt cgtgatgata atcgattgga tagtctacac ctgttttttt
    23461 cagccattca aaattcttcg gacttgcagt cgcgtagact tctaggccag cccaatgcgc
    23521 tagttgattc aaaatcgaac cgacgccccc ggatccgttg atcactaaga tttttttccc
    23581 gcgattggca ttttcttctg gagttagacc aaatttttca aataacaatt cataagcggt
    23641 caatgaggtc aacggcaagg ctgcagcttc ttcatctgaa agattttttg gtgcaagagc
    23701 gacaatacgt tcgtcaacta gttggtattc ttgattgctt cctgctcttg ttgttgtacc
    23761 tgcgtaaaaa acgcgatcgc cgacagaaaa tttttgcact tcgtcaccta cagcaacaac
    23821 tttcccgact ccatcaaaac ctaagatacg taaagcattt cttatcccgt tatcttggcg
    23881 aagtttcgta tcgacaggat taacagatac tgcgttgact tttaccagaa gatcccgtga
    23941 actgggtaca ggtatctgtt ctttttcatc aataaagctt tttggatcat cgatcggcaa
    24001 cccttcataa aaaccgacta ccttcattaa ttccttttct gacatacgaa ttcctcctta
    24061 gcttcatcaa gttggagcat ccatacagtg ttcatttcaa tatatatagt atgctatttg
    24121 ttccaccttt tttattcatc ataattattc tcttacttaa agtaaactat ttgcactttt
    24181 ttggttaaaa aagaggttgc gacaagactt ttgtcacaac ctcttataaa cggtgttctc
    24241 ctaccaactc ttcggtcaca agtcaaaata ttcatcaaac atgagaagct gttttttgaa
    24301 attgctcctt gtgactcaga gttaaacggc aggctcacaa cctctgatat aaacggtgtt
    24361 caatcaactg accctcggta ttagctcaaa aaacgacaaa atatgaagag ctatttttgt
    24421 ttggctgttc ttattacttg atacaggaca gctttttcac gacctctaat tcacggtgtt
    24481 caagcaagaa atcattgttc actgaaaagc gttggatgtt ctttttcaaa ggtctctaag
    24541 atctctcgta cttcatcagt agatttcgta ttcatcaact gaactcgcaa gtcatttgct
    24601 cctggaaatc ctttgatata gattttaaag aaacgatgta gtcctgtgat ggaacgcggg
    24661 atgatttctg aataatgatc ttgcagatct agttgcagac gaagtagtcc gatcagttct
    24721 ttttctgtat gttccctcgg gtctttttca aatgcataag gattcttgaa aatccctcgg
    24781 ccaatcatga tgccatcaac gccatatttc tcggccaatt cttctcctgt ctggcgatcg
    24841 gggatatctc cattgatcgt gattagtgtt tgtggagcga tttggtcacg taaagcaatg
    24901 attttaggaa tgacttccca atgtgcatct actttactca tctctttacg cgtacgcaaa
    24961 tggacagaaa gattggctat atcttgatga agtaggtgac tgatccattc ttccatctca
    25021 gacatttccg tatagccgat acgcgtcttg acgctgacag gcaaaccgcc agctttagct
    25081 gcttcaatca attgtgccgc tacttctgga cgaaggatca agccactgcc ttttccacgt
    25141 tctgctacat tagggactgg gcagcccatg ttgatatcga ttccttggaa gcccatttct
    25201 gccatagcaa tgctcatttc tctaaaaaat tctggcttgt ctccccaaat atgggcaacc
    25261 atcggctgtt catcttctgt aaatgttaat cttcctcgta cactgtcttt tccatctgga
    25321 tgacagaaac tgtctgagtt tgtaaactca gtaaagaaga catccggtgc tgctgcatgc
    25381 tttacgacat ggcgaaatac aacatctgtt acatcttcca ttggtgctaa aacaaaaaaa
    25441 ggctttggca gctctttcca aaaattagta gtcatttatt tgttactctc cattctatca
    25501 agcagtgaag tcgcaatctt tctcaaaaac attgtcatta tactaggaaa caaaaaaaga
    25561 agcaaagtga acagcttaaa agatgtttct gattgagcaa aaaaaagact gtgacaaaaa
    25621 tcttatcaca gtccattttc cggaataaat ggtgttcaaa agcttgcatt tgcggtaata
    25681 agtccaacca aacgaaaaat aggaggagct atttttgttt ggctgttctt attacttgat
    25741 gcagaacagc tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta
    25801 ttcgctcaaa aaacaacaaa atatgaggaa ctattttccc gtttgtttct ctaatactca
    25861 ggtcaaaacg ctgatttcac aacctctttt ctaacttctc aatcctcttc ctttattgat
    25921 caaataccaa gtaacggcgt acaaaacaac gatgaataac acaagtacga tcatcgatgt
    25981 tgctactggc acatcgatcg ttccaaggaa tccgtaacgg aaaccggaga tcatataaac
    26041 gatcggattg acttttgaaa cagcttgcca aaagggcggt aacatagaga tggcatagaa
    26101 aacgccacct agataagtta acggctgcaa gacaaatgta ggcacgatcg atacatcatc
    26161 ataagactga gcaaaaatcc cgttgagcag tcctgctaat gaaaacaaga ttgccgtcat
    26221 caataatgta atgatgacca tgaaccatga atacacatga agtggtacaa agaacaagga
    26281 aatgatcgtt acgagagagc cgacgagaat actgcgaccc acaccaccaa tcacaaagcc
    26341 ccaaataatc acatgcgtag gaacaggtgc taccaagatt tcttcgatat ttttctgaaa
    26401 tttttgagag aagaaggacg aagaaacatt ggcatacgaa ctagtgatag cagacatcat
    26461 aatcaaacca ggaacgatga attccatata tgaaaaacca cccatgtcac cgatccgccc
    26521 accgatcatt tttccgaaaa taacaaaata caaggatgtc gtgatcactg gcggaaccag
    26581 tgtttggacc catatacgca aatagcggtt cgtttccttg atagctaaac tttttaatgc
    26641 agtaaaataa agattaaaca tttttctcct ccgtatgatt attttcttct gtgattttca
    26701 agaacagttc ttctaaacga ttcgacttat tcctcataga aagaactttg attccttgtc
    26761 ttgtaagctg atcgaagatt ccgttcaccc cttgatttcg ttcaacttcg acagcgattg
    26821 tctgctcatc ttcaaatgta caagcatatc cttcaatcac aggtttggta tcatatggcg
    26881 ctaaatcaaa gataaatgtt tcaaattgca gttttgacag aagctctttc atacttgtat
    26941 tctcgatcag ttcacccgat tggatgattc cgatgttgcg acaaagcatt tcagcttctt
    27001 ctagataatg cgtcgtcaaa atgatcgttg taccattttc gttcaattct cgtagaaact
    27061 cccacatctc gcgtctcaac tcgatgtcaa ctcctgctgt tggttcatcc aagatcagca
    27121 aacgtggttc atgcatcaat gccctagcaa tcatcagtct tcgcttcatt cccccggaaa
    27181 gcatacgtgc acggacattt ctcttttccc aaagattgga ctgcttcaaa tatttttcgc
    27241 tgcgtttcat cgcttctttg cgagagacac cgtagtagcc tgcttgattc acgacgattt
    27301 gctggaccgt ttcaaaaggg ttaaaattga actcttgtgg aaccaatcca atctgttgct
    27361 ttgctcgaac tagatccttg tccagatcat aaccgaagac attcactttt ccagatgttt
    27421 tattgactaa agaagtaata atcccgatcg ttgtagactt cccagctcca tttggcccta
    27481 atagcgcata gaaatctcct tctttcaccg aaagatcgat tccccgcaat gcttcgaccc
    27541 cacttccata gatctttttc aaattattta tttcaagcgc atttgccatt tatttcctat
    27601 ctccttatag acatgattct taattgtatc atggaataca gcagatgaaa aaacaacttg
    27661 gcttccacac aaaaacacac ctgctaacaa agaggagatg tgttttctag ttcatgatca
    27721 tcttcgatag aataactatc taaacaactc tggaactttt aaaatcataa aagtaccgac
    27781 agcaactact acgatgacat atagaagatc cttataactg aggttcttgt cttttttatt
    27841 catgttaagc tttcctccgt ccttttatcg aaagcttttt tcttttattt taaccgaaat
    27901 aaataaaatg gaaatagtta ttttcaattt ctgttttcgc tgtttgtttt acggagaact
    27961 ttgccatgga acgtgcgtct cgtcgctgat gctaagtttt ttcgataaaa gctgctagaa
    28021 ggaagacagc gtggtttatt ttgtatcgtc ttttgctaaa tcttccaaga tcatctggac
    28081 agcagactcg ttcccattga attctgccac ttttttggca tcaatgtact tcttttgata
    28141 atcgtctttt tggacccacg catcatgctt cgtttgataa agttaatcaa acggatacca
    28201 ctgacgctgt tcatcgaaca acaaactttc tttttgggcc tgttcaacta tggatttagg
    28261 ataattcaaa actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa
    28321 tttgaaatca aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc
    28381 acaataattc tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga
    28441 tggatactcc gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt
    28501 tgttcctttt aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc
    28561 cagcaaacgt ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc
    28621 atcttcgatt gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat
    28681 cgctgtttgg atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga
    28741 ttttcctgat gcattggaac ccgtgactaa tgtattcttc tgccagttga ctggattaac
    28801 taccgcttct tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa
    28861 ctctggtcgg caagtgatcg gcatataggt gcgataattc aaaacggctg ctgcaacttc
    28921 caattctccc agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt
    28981 gagaacgaaa tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat
    29041 caattctgct tcactcccgc tcttcattcg aaatgaaaaa cctaatttag ggatgtctct
    29101 taatggttta gtttgctgtt tcagctcttt ttgaagtggt gtcgtcattt ttgctaactg
    29161 attggcggct gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt
    29221 ttgtttcttc actagataat agaccgtatt gaatactaag ccaccgattg ctagatagat
    29281 tcctagtact tgccctaaaa gaagcaaacc tacacccacc agcggaagca agccaagaaa
    29341 aataaataaa cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc
    29401 aaaattatga tctttttttc ctaaacgagc gaattgatat tggattcttt ctctcagttg
    29461 aggattttcc tgataaaaag caattagttt ttctaactga taatcttctc caaaatcaaa
    29521 gctacgtaaa cgctgataca acgcttctga acctacacta gaataggtac tgttgatccc
    29581 ttcaaatact tcgaacatat ccaagtcata ccaagtaaga tcgtcgattt cactatccca
    29641 tgaacggaat tttttctccg tgagccacgc atccttcaaa ctttcttctt tatcaaatct
    29701 cggttgataa ggaatttttc cccaattact acgaactgtt tcctttactt tcaaacgtga
    29761 aaaaatgtct aaaacaacta ataaaataac aactgcaata ataattaaaa cgataaattc
    29821 tctttggccc atatccctct ccgttccttg atttgttagt tttagtatag cactgttgcg
    29881 ctatttgaaa agggggtaca aaaataactg tcttcgttac ctaattcgta tcaatcacac
    29941 caaaataaaa acaccttcca aaaaagaaga tgtctttaaa acgagataca attttttgtc
    30001 attcacttca ggtaaattag agccaattaa accctccgca acttgacgga gatggagaag
    30061 atgcccccgt ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac
    30121 aggtaaagcc cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa
    30181 cgctggaaga ctttttcaac agaggtttgc gctcctggtt tgataggatt ggccactgtt
    30241 aatcgaatga atgaatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc
    30301 aataaattct gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca
    30361 gttgtattta tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca
    30421 taaaaagaga aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt
    30481 tctttttgct tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat
    30541 cttgcaacag attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc
    30601 tgttccagta attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa
    30661 aggccgacga tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt
    30721 tcattttcat ccacccatgt attcagtgca tctactaggt ccattagatt cttttctcga
    30781 aattcgactg aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta
    30841 gttaatctct tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg
    30901 agaagcaaca gccacttaat caaatctaaa ccccactccc cacaccgtat gaatatacac
    30961 agcatgtgcc tttgctagtt tccctcgaag attggaaata tgcatattca ctgttttctc
    31021 actatcaaaa tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt
    31081 tttcggatgt tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc
    31141 cacaccattc acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat
    31201 gcgttgcgac agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg
    31261 taacaacagt tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa
    31321 caaggaaact ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga
    31381 tgtctgtcgg atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag
    31441 aaggatcaaa tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc
    31501 atcaagaagt ttgtatctct gttcaagaac ctcctccagt aaagaatgaa cagcttcatc
    31561 gtcttcgata atcaaaatag tttccagcag gaacacctac ttaatgtcag atttttggaa
    31621 aataaacatt cctagagctg atgataccac gattgttact agactgacca gaatcatttt
    31681 cggtgcttgc gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt
    31741 aaaccactcc gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaacgaaggc
    31801 cgttcccatg atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa
    31861 tgtcaaagcc actggaacag ataatgtcgc taatatcagc agaagcaata caggaaatac
    31921 ttttagaaca taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag
    31981 tgtgtaagca atcatggcta gccccgtaat caaaaccacg ccaagtgttg tgatcaaaag
    32041 cgtaacgaga agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt
    32101 gcgaatcgta ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc
    32161 cagaagaatc catgcagcca ttttttccac cattaaaatc cctgttgcac cgttgatgac
    32221 tgtatccgtt gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca
    32281 agaataaaaa agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt
    32341 ccatactttt attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt
    32401 gtaagaaata atcttccagt ttttgcccaa ctaggtggat accatccaca ggaacttttg
    32461 cctctaccaa tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa
    32521 cacggctaga ttggacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt
    32581 gttccgtcgc cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt
    32641 tcgataattc tttgatcaag tgtccttcat gcaaaatacc gtagtgagtg gcgatctgtg
    32701 acaattcatc tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac
    32761 gttggatgat ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat
    32821 ccaaaatcaa aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca
    32881 tacctaaaga gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca
    32941 gcaggtcttc gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat
    33001 ctcttgcagt taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc
    33061 ttgctgcctg caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca
    33121 aacccataat agttctcata aaggtagact tacccgctcc attctcccca atcaaaccat
    33181 agatcattcc acgtttgatt tcgatcgata ctttatctaa tgctttggca gcaccgtatt
    33241 ttttggttat accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc
    33301 tttcttattg ataagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata
    33361 aagaaagtgt aaattttcca attgaatttc aagaaaaatc agactgtctc gcagacgtgg
    33421 ttgagttgtc tgccaatcaa agagcgcacc aaacagtgct aatccaagtg gactggccac
    33481 catggtaatc gttgttatcg ttgacatcag acgtccttgt atatgcgttg gaacttctcg
    33541 ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac ggacttatga
    33601 aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt tatgacaaaa
    33661 gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc ctcggtcaca
    33721 agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt atttcttgaa
    33781 gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga tgatacttta
    33841 tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc tacctattaa
    33901 catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag aaaagttagg
    33961 attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac tcgtgaactc
    34021 gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt tcttaagacc
    34081 tggtttttta cgttcgacca tacgtgcgtc acgagtaagt aagccagcac gtttaagagc
    34141 taaacggaag tcaggatcaa cttctagcaa tgcacgagcg ataccatgac ggatagctcc
    34201 tgattgtcca gcgtatcctc cgccgtttac gttaacaaat acgtcatatg cgccttttgt
    34261 ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag tcagcatgtg gaatgtattc
    34321 ttcaacatct tttttattta cagtaatttt gccagttcct ggtactagac gtacgcgagc
    34381 aactgcgttt ttacgacggc ctgtgccgat atattgaact tgtgccaatg aaattccctc
    34441 ctattagatt aagtttgtga tgtctaaaac ttctggttgt tgtgctgcat gtggatgttc
    34501 tgctccaccg tatacgttca attttgtaaa ttgtttgcgg cctaaagtgt ttttaggaag
    34561 catacctttt acagaagttt cgatcaaacg acgagaattt ttagcacgca attcgccagc
    34621 agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg taataaattt tgtcagtcgc
    34681 ttttttacct gttaatttca ctttgtctgc attgatgacg atgacgaaat cgccagtatc
    34741 caaatgaggt gtgaaagttg gtttattttt accacgtagt acagatgcaa caactgttga
    34801 aagacgtccc aatggaacat cagttgcgtc tactacatac catttacgtt ctacttcgcc
    34861 tggtttggcc atatatgttg tacgcacgtt attttcctcc aatttaagtt ctgatgtttg
    34921 acatacactt cgagtttccg gggctctttg tggggcaaac aataccattt aacatattac
    34981 ccgctaatct agtcaatgtc aacgtttttc tgcttgattg aaaaatttat atgaaacttt
    35041 acttcttttc tttcttttat ataaaagggt cgtttcctgt caaaatgaaa aacaaaaaga
    35101 ggctgtgaca aaagtcttgt cacaacccct tattttgaat aaacggtgtt ctcctgccaa
    35161 ctcctcggtc acaagtcaca atatccataa aacataagaa gctgtttttt aaaattgttt
    35221 cttgtgactc ggagctgaac ggcaggctta caacctcttg gtaaacggtg ttcaatcagc
    35281 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttg acgtttgttt
    35341 ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa gttcaacaac
    35401 agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat ccagatacgt
    35461 ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc gccttcttct
    35521 ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc tgcaacaagt
    35581 gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg gatttttgtg
    35641 ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat caagcgttcg
    35701 gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat tgggtgcaat
    35761 gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac agacatttgg
    35821 cttagatctg caactaattg atttaatact tctttggttt gttggaattt catctttctt
    35881 gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa agtacaacaa
    35941 aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc aatcattttt
    36001 cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt gctaaatgct
    36061 tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca gtttcttgta
    36121 ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt tggatctttc
    36181 cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg acagtaggga
    36241 aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga gattgagcaa
    36301 acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct tttaaaatcc
    36361 gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt tctgtatgtt
    36421 gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt gctcgctcac
    36481 caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa gcgatagccg
    36541 aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg acttcctgta
    36601 aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct cctcccacta
    36661 cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg aactgaggct
    36721 gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg atcgtatcac
    36781 caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa acaaatgata
    36841 tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt acttgatagg
    36901 tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca agcacttctt
    36961 ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt ccgtagtcgc
    37021 tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca aaataagcag
    37081 tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc aatggtgtca
    37141 ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg gtatatttca
    37201 gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga gataacggtg
    37261 ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag acaaattgaa
    37321 tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag acaccttgaa
    37381 agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa gggtagcgaa
    37441 aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg gtttgtgtgg
    37501 ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta tgtatgtaca
    37561 aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc gtgttacttt
    37621 attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag cggattccgt
    37681 taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt tcgctttctt
    37741 ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc cgctattgca
    37801 ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt ctttttgcct
    37861 ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg ctttcggctt
    37921 tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg ctattctgtt
    37981 cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg caaagtgtgc
    38041 tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat ctcggagctg
    38101 gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag ttgcttcaga
    38161 tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt atctgtcata
    38221 aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg ctgattgtcc
    38281 ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct ctgatatata
    38341 gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca acctctccac
    38401 taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt ttacaacctc
    38461 tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg ccccaatgtc
    38521 gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg agcttgtttt
    38581 tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt ggttcgattt
    38641 ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt gaacgctctg
    38701 caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa tcaccgcgat
    38761 cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt tccaccaact
    38821 gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta tgccctgttg
    38881 tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta taaccggatg
    38941 tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa aagcatccgc
    39001 ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca taccttgatt
    39061 tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt atatacgtga
    39121 tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta aatcaggaat
    39181 cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat gcatgttacc
    39241 agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg catttggcgg
    39301 tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg cttttgatac
    39361 ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt ctaaaatgag
    39421 gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt tcaagtactc
    39481 aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa tcgcttgaag
    39541 atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga atttctgttc
    39601 catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc ctttcagttg
    39661 ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca gccacgcttc
    39721 gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg ctgctttgat
    39781 tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat gtttttagct
    39841 gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac agctgcagga
    39901 aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc tttaccaaca
    39961 cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat cccaactggc
    40021 gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac gaggtctgct
    40081 tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc agagactatt
    40141 ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact actgcccgat
    40201 gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg gtcagccact
    40261 gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc gccttctttt
    40321 ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat cagctcaata
    40381 ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag tgcttctact
    40441 ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc gatgccgcca
    40501 tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg ttgcaacaat
    40561 tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg ttggtttagt
    40621 gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg accgtctgaa
    40681 gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt ttgccgcttc
    40741 aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc tataggtagc
    40801 agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc ttgtgctgcc
    40861 tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg cattattttc
    40921 ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg acaaaaatag
    40981 gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa acaaatagca
    41041 acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa ggaagtcttg
    41101 gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat ataactgcta
    41161 gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc ggatctggtc
    41221 ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt tcttggatac
    41281 ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt gttttgtttt
    41341 catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt aataagtata
    41401 acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg ctacgggcat
    41461 aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa tcaataactg
    41521 gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc actggcaaat
    41581 ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca gcttgatatt
    41641 taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta ttgtcgatat
    41701 attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca tcgactttta
    41761 cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc tttgtttcta
    41821 aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca aggtctgcac
    41881 tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt ttgatattca
    41941 atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat gggaaaagtg
    42001 caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga tcataacggt
    42061 acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc aggataaaaa
    42121 tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc ttcgccccta
    42181 cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca gcgaagtcca
    42241 tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga tgccaaaaat
    42301 ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt ttctgggctt
    42361 gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc acgatgatct
    42421 tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg gtgatccggc
    42481 gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca atatcaattt
    42541 tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact cgcagttctt
    42601 tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc gttttcacat
    42661 ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc tgatggagat
    42721 tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga gcagtttcaa
    42781 tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg tatgcaagaa
    42841 ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat ctgatgacat
    42901 ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat acaccagctg
    42961 caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg ataccaaaaa
    43021 atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa tcaaactgtt
    43081 tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat ataaattttc
    43141 caccagattc tggatgacat atccttgcca gccatctgca atcatctgag tctggtcacc
    43201 gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa ctggcgtggt
    43261 cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg gatacagatt
    43321 agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga tattcaaagc
    43381 aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc caaaaattgc
    43441 atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat tttttttcgt
    43501 accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc cagtaatata
    43561 catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc cttgaagttc
    43621 tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat tcgcttctac
    43681 gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt cgtcagaaaa
    43741 acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct ctgccatctt
    43801 tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag cattagcttc
    43861 gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac tcacgatatc
    43921 gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa atcctgtacg
    43981 tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca attctaagac
    44041 atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc cagcgcctat
    44101 gattgctact ttcttcattt atttctccct cctttgctta tttacttatt gttattcttt
    44161 ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt tttcaagtat
    44221 tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca ttcatatacc
    44281 atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa atcaattgcg
    44341 ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac atactctttt
    44401 tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata aaaatcagcg
    44461 atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc cgctaacaaa
    44521 ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt acctgtttct
    44581 tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc tccttctttg
    44641 cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc tgctgggata
    44701 acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat ttgacgtaca
    44761 ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc gttacaagct
    44821 gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat attttctatt
    44881 aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa tctaaatttt
    44941 tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat tgttctcctc
    45001 ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca taacgcatgt
    45061 aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca caatccaagt
    45121 aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc catcttgggt
    45181 aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta ttgaaatgtg
    45241 cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca taagaaatga
    45301 tcccgccata gccgatccct cttaaagaag cttgtaatgc ggcagcactg ccactcgttt
    45361 caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa ccgacatctg
    45421 ctttcatagg atctagcgtg taatcggcac cgtatttttc tgctaattct ctacgctgcg
    45481 ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat tgaatcgcaa
    45541 tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga acatgtgctt
    45601 cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt ttccattgat
    45661 ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc agcgtttcac
    45721 gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc aaactgcccg
    45781 tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat accacaccgc
    45841 gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat tcttcatcga
    45901 taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga gtagcgaaaa
    45961 ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct ttgaatccgg
    46021 ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt cttttctttt
    46081 tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga taacgcatac
    46141 ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc ccaaatgtag
    46201 tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc ggaggtcgac
    46261 aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata aacgaagaca
    46321 gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg gatccggcta
    46381 agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa ataacttttt
    46441 agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag tgctgtattg
    46501 gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat caaaataccg
    46561 gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt gaagcgtagt
    46621 tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga ggctatcaag
    46681 gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc attgaaaata
    46741 tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt tgcaattgct
    46801 tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa attgacagct
    46861 aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt tttcatttca
    46921 agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac aatagaatcg
    46981 atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac aaataaacgc
    47041 ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag aacatccaga
    47101 tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt gtcacgatct
    47161 tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc gttttgtcga
    47221 ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc cagatagcaa
    47281 acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt gcttcgttga
    47341 aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta ttgaacatat
    47401 atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa atcagtgaac
    47461 gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct aatcctaaag
    47521 ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa gaacgataaa
    47581 attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg gcgtctgagt
    47641 taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt ggaatcagca
    47701 ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat tttaagtcag
    47761 aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat agctatctac
    47821 ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt gcgtcagcta
    47881 aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt ccgatcacac
    47941 ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt ccaaagactt
    48001 gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac ttgatgactt
    48061 cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga gcaccaccag
    48121 gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct gtgaatgctt
    48181 cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca gatactgtgc
    48241 catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct gagttccccg
    48301 cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct gttccattca
    48361 tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat tgggataagt
    48421 tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc tcgccttcat
    48481 catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc tcactcattg
    48541 ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct gtgaaatgtt
    48601 gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct tctgataatt
    48661 tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca atatagcttt
    48721 ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct tctccagctt
    48781 cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc atcactttag
    48841 caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct aacgtaacga
    48901 tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg tctggatttt
    48961 ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt tcgacagaag
    49021 agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt tcaccacaag
    49081 cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt ttcatttttt
    49141 tccacctctt attttattat tttcagccac atatattcat acggctggaa tatcaacttt
    49201 cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc tccttttggt
    49261 aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt ctcttccttc
    49321 gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat caaactattc
    49381 ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc accatatact
    49441 gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa aaatttttgc
    49501 cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt tcgacttccc
    49561 agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa tgaatgagca
    49621 gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa cgcatctaga
    49681 tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt gtagtttact
    49741 cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc cggcagaata
    49801 cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa aaaagttgtt
    49861 tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt attacccgaa
    49921 cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg tgcttcattc
    49981 tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc agtgatcaat
    50041 accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat tttatgcgtt
    50101 tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac agcgtccaag
    50161 cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat tttcaataaa
    50221 acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt ccatacgtgt
    50281 ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct tggtctagtc
    50341 acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta cgtatcattc
    50401 aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca gacaaagtcg
    50461 aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc caaacggtcg
    50521 ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg gaaaaatggc
    50581 aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt cttaaatgta
    50641 cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa gaaaatatct
    50701 tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac actgactttt
    50761 tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat cgcttgtaaa
    50821 agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg atttgatcat
    50881 attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata tacacaaaaa
    50941 tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat atggtctctc
    51001 atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa tgtctattgt
    51061 actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt gaaacctact
    51121 atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag agtattaaat
    51181 aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat caaagaattg
    51241 aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga cgcgattcta
    51301 gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc agatgttatt
    51361 caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca aacggcaaaa
    51421 acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat gatccgagga
    51481 attattattt taagctctac gatggatgaa acacttcttt ttgaattgga tcaatacaat
    51541 attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta ctcagtggac
    51601 acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa tggacaccgt
    51661 catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg tctcaatggt
    51721 taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat tatagacggc
    51781 ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct acgagaaaga
    51841 ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa agtggcagaa
    51901 caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga tcccttattg
    51961 tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca gttaggaatc
    52021 aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga aaaaagaatc
    52081 attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat gtagaacaga
    52141 attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt ttcaatcact
    52201 catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc atctgtttta
    52261 gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg gaagaaaaaa
    52321 ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac acatttaccc
    52381 ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag aataacagac
    52441 atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca aaatattaaa
    52501 aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc tatctttact
    52561 tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg atacgaaccg
    52621 atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt agacaaacca
    52681 aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct attttgtttg
    52741 attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac tgtagacatt
    52801 ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca taccagtacc
    52861 agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag gatctttctt
    52921 accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg cagcagataa
    52981 gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc gagaagttgc
    53041 aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg cgatgtctaa
    53101 taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt tacgcagcat
    53161 ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca tacggtaaac
    53221 acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa cttgcaacaa
    53281 ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca tatcgccttc
    53341 tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg tttttccttt
    53401 gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga catcccctgt
    53461 tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt cttggacacg
    53521 aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg tacgcatcgt
    53581 caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg cttctccgac
    53641 ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga caccatgttt
    53701 tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat caacgatttg
    53761 ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat ttgtttcagg
    53821 atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg attcgatgat
    53881 ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc aatcttcttc
    53941 acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac ctgaatcggc
    54001 agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga taaacatttc
    54061 caagacggat agtccttcac ggaagttcga aatgattggc aattccatga ttcgaccatt
    54121 aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa tgttaccacg
    54181 agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt ccatcagttt
    54241 ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat aacgttcgtc
    54301 atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt tatgggcatt
    54361 ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta ctgtgatacc
    54421 ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct tagaagtttc
    54481 tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt tcttcttgaa
    54541 cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag cttcaacaaa
    54601 gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca gataagggaa
    54661 ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca tcgtacgttc
    54721 tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac cgatacgtga
    54781 atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat ctctgaagac
    54841 cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac ctagaaccat
    54901 atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt tttgagcagc
    54961 taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt ggacagccat
    55021 ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca aacggattgc
    55081 gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa gcgtaggtgc
    55141 ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc atacttcgtc
    55201 ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt cgcgttgaac
    55261 aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg gaagtccaca
    55321 ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt agtcgacacg
    55381 tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat gagaaagaga
    55441 tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt tatcgatcaa
    55501 tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc caggtgcatt
    55561 caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt aaagatcatt
    55621 caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta aatctggtgg
    55681 aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag cgcggaatgc
    55741 ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg cagatttcaa
    55801 ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta attgtttaat
    55861 cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt tttcgcgata
    55921 ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt cgatgactac
    55981 atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta atacaagacc
    56041 catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg ctaattcaat
    56101 atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc atcgatcaca
    56161 aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc agtcttttgt
    56221 aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg tacgatagtt
    56281 aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt cgggagaagc
    56341 caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt ccctccattt
    56401 catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc atagaccgga
    56461 ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt tttcatcttc
    56521 cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg caaattttgt
    56581 caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa gttcgatctc
    56641 tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt ctttcaccaa
    56701 tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga cgattgcttc
    56761 gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt cttgtaaagt
    56821 ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac gttgtccacc
    56881 aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc cgattgaacg
    56941 tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca caccgacaga
    57001 cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt tcgcatcgct
    57061 ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac catcaaatac
    57121 tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta attccaatac
    57181 ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat cgattggtgt
    57241 tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa cccctttatt
    57301 tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa cgatataaac
    57361 acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag taaagatttt
    57421 cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat cgcggacttc
    57481 acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata attctgttac
    57541 cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg caccaatacg
    57601 gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag ggatttcgcg
    57661 tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt cttcaatatg
    57721 gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg catcttcata
    57781 gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag ccatttcgcc
    57841 ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct cacccaaatg
    57901 aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt ttgttacatc
    57961 gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat caacgtattc
    58021 aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg atttgtattc
    58081 cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt gacgctgcat
    58141 gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa tacatgctgt
    58201 cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat caatagatac
    58261 ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg ttccatcttc
    58321 attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag ctgtcaagta
    58381 gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag gtgtttcgat
    58441 aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc cgatgtttgg
    58501 gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa cgtcacgaac
    58561 ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa gacgacgttt
    58621 gtatgtcaat tcgccaagag ggtttgtttg atccacgaac tgtgacaact gagaagaacc
    58681 aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt gaggtgtcaa
    58741 tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac gtgctagccc
    58801 gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat ttcctaagtg
    58861 gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc tcattgaagc
    58921 aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt ttccgatcac
    58981 gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga cagtcattgg
    59041 atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc cattatcaat
    59101 gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt caacgatgat
    59161 ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata gacgtgtttt
    59221 caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt aacgttttgg
    59281 atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg gttcgcctgg
    59341 acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc ttgcgttttt
    59401 atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga tttcaaagat
    59461 cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa tcttacgtgt
    59521 acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta gccaagctcc
    59581 acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga cttttccatg
    59641 gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg ccccgttgat
    59701 gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt cttgtgcttt
    59761 gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg cagagtagtt
    59821 tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt cataatctac
    59881 aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt caaacatttc
    59941 tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga tcaaatttgg
    60001 taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt ttccgtattt
    60061 tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa gactacccga
    60121 tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca ttttttgcgg
    60181 cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact tcgtgagctt
    60241 ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct gccttttctt
    60301 catcagatag tttttgcaac ttcatatact acacttattt tctataaaag tcaacacttc
    60361 ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt atgttttcaa
    60421 ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta gaaaattcat
    60481 tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca caactgaata
    60541 acttttactt tagctacttt agataggttt gtattcatca tgatggatac aaacgttaaa
    60601 cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag ccacgtagac
    60661 aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca ttctactttt
    60721 tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg attttttgtt
    60781 ttccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat taggcgctgc
    60841 cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg catcagatgg
    60901 acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg ctgcctataa
    60961 aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc taacaatcga
    61021 ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga aactcgtcaa
    61081 agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt tattaaatga
    61141 aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag cccatttagt
    61201 cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg tgccaatcga
    61261 aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg gcatcatgta
    61321 taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag aattgaaaaa
    61381 agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc aacaagtgac
    61441 gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca cgttcgcaag
    61501 tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg ttgctggcgc
    61561 agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg aaagcttggg
    61621 caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa gtgaacttcc
    61681 tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca aagcattagg
    61741 catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct catgacaaat
    61801 ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct acttgcgatt
    61861 ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga aggaagcttt
    61921 cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc attgacagct
    61981 tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact gcttcacaca
    62041 aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt atattctgtc
    62101 acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac gatctttcat
    62161 tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg tctgcttttt
    62221 gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg acttgctatt
    62281 cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac agataatatg
    62341 aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc cttgcttgcg
    62401 caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat tggacttgct
    62461 tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg tctcttgact
    62521 gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga actgaatgtt
    62581 gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct ttctttcccg
    62641 cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt caaatgaatg
    62701 acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc aacgaaaatc
    62761 atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg atcatcggtg
    62821 gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt tctgtcgatc
    62881 agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc gaacgttcga
    62941 aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt ttaacggtag
    63001 aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc cgtggtgtca
    63061 aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg gggttggaaa
    63121 atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct attacgttat
    63181 tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact gcagcgcttg
    63241 accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact gagcagaatc
    63301 tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg agtcgactga
    63361 tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa cagcagctca
    63421 cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta aaagacgatc
    63481 aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg cccaccctct
    63541 tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt ttcttcgaaa
    63601 actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt gatgccatga
    63661 caacgaaaga caaggtactt caattattga aaaagacacc agattttctt tccggagaaa
    63721 agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc aaagaattgg
    63781 aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg ccctctgacg
    63841 tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat ttgaaagtac
    63901 aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca gctcaaggag
    63961 attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga cgttttggac
    64021 ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc catccagaca
    64081 aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt gtgaaagcca
    64141 ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc tacatggatg
    64201 gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc ggcaggatca
    64261 gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat tttccagctg
    64321 atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc acaaggaatc
    64381 agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca gaaaaagcgt
    64441 tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc aatcaaaaag
    64501 gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg atcgtccaaa
    64561 caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct atccgtacgc
    64621 aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt ttttctgaat
    64681 tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct ctagaaaaag
    64741 gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca atgcaaaaaa
    64801 caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct ttttcaatct
    64861 tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta ttttgccgta
    64921 agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg catagatcac
    64981 ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc ctcgtgtgac
    65041 tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca gccttcttaa
    65101 gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt ctatttgatt
    65161 agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt tatctgctat
    65221 cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag ttcctgctaa
    65281 atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag tcattgattt
    65341 gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag agacgccttc
    65401 aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca tatctaaata
    65461 atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt ctaggatacc
    65521 tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca tcatcaatag
    65581 tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa gcaacaatat
    65641 gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca gtttaagact
    65701 aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa aaatgaaaaa
    65761 aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt atggtgtggc
    65821 cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg atctaactgg
    65881 taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa ataagacaac
    65941 tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa aaaataattt
    66001 tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac aaaaaactga
    66061 ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata aatttgtcgt
    66121 tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg gatccaggct
    66181 tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat atattgattt
    66241 cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga tcactccagc
    66301 taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg atttaactga
    66361 tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa cgatgaatac
    66421 gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca tcaacagttc
    66481 ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc atgaaacaga
    66541 agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg atttgacaat
    66601 cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca ttactcaaaa
    66661 ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca atcgttggga
    66721 tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa gcgacagtac
    66781 agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca gtacagatac
    66841 tacctctacc acaagtgata gcacggatac atcagcttca agcgacagta cagatactac
    66901 ctctaccaca agtgatagca cggacacatc agcttca
//
LOCUS       DAZKSU010000006        66155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000006 DAZKSU010000000
VERSION     DAZKSU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.006"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(165..464)
                     /locus_tag="ACG8Z3_000393"
     CDS             complement(165..464)
                     /locus_tag="ACG8Z3_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0822742.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            815..1447
                     /locus_tag="ACG8Z3_000394"
     CDS             815..1447
                     /locus_tag="ACG8Z3_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0822743.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1570..2208
                     /locus_tag="ACG8Z3_000395"
     CDS             1570..2208
                     /locus_tag="ACG8Z3_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0822744.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2327..2399)
                     /locus_tag="ACG8Z3_000396"
     tRNA            complement(2327..2399)
                     /locus_tag="ACG8Z3_000396"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2364..2366),aa:Thr,seq:cgt)
     gene            2520..2762
                     /locus_tag="ACG8Z3_000397"
     CDS             2520..2762
                     /locus_tag="ACG8Z3_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822745.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2901..3530)
                     /gene="upp"
                     /locus_tag="ACG8Z3_000398"
     CDS             complement(2901..3530)
                     /gene="upp"
                     /locus_tag="ACG8Z3_000398"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0822746.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3664..4611)
                     /locus_tag="ACG8Z3_000399"
     CDS             complement(3664..4611)
                     /locus_tag="ACG8Z3_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0822747.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4764..6008)
                     /gene="glyA"
                     /locus_tag="ACG8Z3_000400"
     CDS             complement(4764..6008)
                     /gene="glyA"
                     /locus_tag="ACG8Z3_000400"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0822748.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(6183..7208)
                     /locus_tag="ACG8Z3_000401"
     CDS             complement(6183..7208)
                     /locus_tag="ACG8Z3_000401"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0822749.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(7230..7409)
                     /locus_tag="ACG8Z3_000402"
     CDS             complement(7230..7409)
                     /locus_tag="ACG8Z3_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822750.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(7402..8241)
                     /gene="prmC"
                     /locus_tag="ACG8Z3_000403"
     CDS             complement(7402..8241)
                     /gene="prmC"
                     /locus_tag="ACG8Z3_000403"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0822751.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(8234..9307)
                     /gene="prfA"
                     /locus_tag="ACG8Z3_000404"
     CDS             complement(8234..9307)
                     /gene="prfA"
                     /locus_tag="ACG8Z3_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0822752.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(9310..9894)
                     /locus_tag="ACG8Z3_000405"
     CDS             complement(9310..9894)
                     /locus_tag="ACG8Z3_000405"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0822753.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(10000..10248)
                     /locus_tag="ACG8Z3_000406"
     CDS             complement(10000..10248)
                     /locus_tag="ACG8Z3_000406"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0822754.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            complement(10239..10469)
                     /locus_tag="ACG8Z3_000407"
     CDS             complement(10239..10469)
                     /locus_tag="ACG8Z3_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822755.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            10575..11177
                     /locus_tag="ACG8Z3_000408"
     CDS             10575..11177
                     /locus_tag="ACG8Z3_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0822756.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(11281..11850)
                     /gene="lepB"
                     /locus_tag="ACG8Z3_000409"
     CDS             complement(11281..11850)
                     /gene="lepB"
                     /locus_tag="ACG8Z3_000409"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0822757.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(12080..12847)
                     /locus_tag="ACG8Z3_000410"
     CDS             complement(12080..12847)
                     /locus_tag="ACG8Z3_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0822758.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12844..13323)
                     /locus_tag="ACG8Z3_000411"
     CDS             complement(12844..13323)
                     /locus_tag="ACG8Z3_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0822759.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(13448..14185)
                     /locus_tag="ACG8Z3_000412"
     CDS             complement(13448..14185)
                     /locus_tag="ACG8Z3_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0822760.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            14346..15746
                     /locus_tag="ACG8Z3_000413"
     CDS             14346..15746
                     /locus_tag="ACG8Z3_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0822761.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15969..17321
                     /locus_tag="ACG8Z3_000414"
     CDS             15969..17321
                     /locus_tag="ACG8Z3_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0822762.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            17314..18012
                     /locus_tag="ACG8Z3_000415"
     CDS             17314..18012
                     /locus_tag="ACG8Z3_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0822763.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(18074..19225)
                     /locus_tag="ACG8Z3_000416"
     CDS             complement(18074..19225)
                     /locus_tag="ACG8Z3_000416"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0822764.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(19238..19675)
                     /locus_tag="ACG8Z3_000417"
     CDS             complement(19238..19675)
                     /locus_tag="ACG8Z3_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0822765.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19690..21456)
                     /locus_tag="ACG8Z3_000418"
     CDS             complement(19690..21456)
                     /locus_tag="ACG8Z3_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0822766.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21720..22397
                     /locus_tag="ACG8Z3_000419"
     CDS             21720..22397
                     /locus_tag="ACG8Z3_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0822767.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            22400..23527
                     /locus_tag="ACG8Z3_000420"
     CDS             22400..23527
                     /locus_tag="ACG8Z3_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822768.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23727..24167)
                     /locus_tag="ACG8Z3_000421"
     CDS             complement(23727..24167)
                     /locus_tag="ACG8Z3_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0822769.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(24178..26211)
                     /locus_tag="ACG8Z3_000422"
     CDS             complement(24178..26211)
                     /locus_tag="ACG8Z3_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0822770.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(26213..27916)
                     /locus_tag="ACG8Z3_000423"
     CDS             complement(26213..27916)
                     /locus_tag="ACG8Z3_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0822771.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(28010..28960)
                     /gene="manA"
                     /locus_tag="ACG8Z3_000424"
     CDS             complement(28010..28960)
                     /gene="manA"
                     /locus_tag="ACG8Z3_000424"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0822772.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28999..30024)
                     /locus_tag="ACG8Z3_000425"
     CDS             complement(28999..30024)
                     /locus_tag="ACG8Z3_000425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0822773.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(30233..31102)
                     /locus_tag="ACG8Z3_000426"
     CDS             complement(30233..31102)
                     /locus_tag="ACG8Z3_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0822774.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31806..33698)
                     /locus_tag="ACG8Z3_000427"
     CDS             complement(31806..33698)
                     /locus_tag="ACG8Z3_000427"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822775.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33695..35422)
                     /locus_tag="ACG8Z3_000428"
     CDS             complement(33695..35422)
                     /locus_tag="ACG8Z3_000428"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822776.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(35438..36088)
                     /locus_tag="ACG8Z3_000429"
     CDS             complement(35438..36088)
                     /locus_tag="ACG8Z3_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0822777.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            36292..36588
                     /locus_tag="ACG8Z3_000430"
     CDS             36292..36588
                     /locus_tag="ACG8Z3_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0822778.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36780..37400
                     /locus_tag="ACG8Z3_000431"
     CDS             36780..37400
                     /locus_tag="ACG8Z3_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0822779.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(37492..38658)
                     /locus_tag="ACG8Z3_000432"
     CDS             complement(37492..38658)
                     /locus_tag="ACG8Z3_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0822780.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38670..39023)
                     /locus_tag="ACG8Z3_000433"
     CDS             complement(38670..39023)
                     /locus_tag="ACG8Z3_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0822781.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            39151..40173
                     /locus_tag="ACG8Z3_000434"
     CDS             39151..40173
                     /locus_tag="ACG8Z3_000434"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0822782.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            40192..40353
                     /locus_tag="ACG8Z3_000435"
     CDS             40192..40353
                     /locus_tag="ACG8Z3_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822783.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(40612..41046)
                     /locus_tag="ACG8Z3_000436"
     CDS             complement(40612..41046)
                     /locus_tag="ACG8Z3_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822784.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(41156..43015)
                     /locus_tag="ACG8Z3_000437"
     CDS             complement(41156..43015)
                     /locus_tag="ACG8Z3_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0822785.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            43016..43156
                     /locus_tag="ACG8Z3_000438"
     CDS             43016..43156
                     /locus_tag="ACG8Z3_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0822786.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(43133..44293)
                     /locus_tag="ACG8Z3_000439"
     CDS             complement(43133..44293)
                     /locus_tag="ACG8Z3_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0822787.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(44600..46024)
                     /gene="araA"
                     /locus_tag="ACG8Z3_000440"
     CDS             complement(44600..46024)
                     /gene="araA"
                     /locus_tag="ACG8Z3_000440"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0822788.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(46040..46738)
                     /locus_tag="ACG8Z3_000441"
     CDS             complement(46040..46738)
                     /locus_tag="ACG8Z3_000441"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0822789.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46695..48356)
                     /locus_tag="ACG8Z3_000442"
     CDS             complement(46695..48356)
                     /locus_tag="ACG8Z3_000442"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0822790.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            48574..49974
                     /locus_tag="ACG8Z3_000443"
     CDS             48574..49974
                     /locus_tag="ACG8Z3_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0822791.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(50226..51305)
                     /locus_tag="ACG8Z3_000444"
     CDS             complement(50226..51305)
                     /locus_tag="ACG8Z3_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0822792.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(51391..52350)
                     /locus_tag="ACG8Z3_000445"
     CDS             complement(51391..52350)
                     /locus_tag="ACG8Z3_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0822793.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(52362..53873)
                     /locus_tag="ACG8Z3_000446"
     CDS             complement(52362..53873)
                     /locus_tag="ACG8Z3_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0822794.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53895..54458)
                     /locus_tag="ACG8Z3_000447"
     CDS             complement(53895..54458)
                     /locus_tag="ACG8Z3_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0822795.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(54464..55327)
                     /locus_tag="ACG8Z3_000448"
     CDS             complement(54464..55327)
                     /locus_tag="ACG8Z3_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0822796.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(55341..56237)
                     /locus_tag="ACG8Z3_000449"
     CDS             complement(55341..56237)
                     /locus_tag="ACG8Z3_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0822797.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(56412..57701)
                     /locus_tag="ACG8Z3_000450"
     CDS             complement(56412..57701)
                     /locus_tag="ACG8Z3_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0822798.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57673..59190)
                     /locus_tag="ACG8Z3_000451"
     CDS             complement(57673..59190)
                     /locus_tag="ACG8Z3_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0822799.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            59388..60278
                     /locus_tag="ACG8Z3_000452"
     CDS             59388..60278
                     /locus_tag="ACG8Z3_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0822800.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(60351..61175)
                     /gene="nadE"
                     /locus_tag="ACG8Z3_000453"
     CDS             complement(60351..61175)
                     /gene="nadE"
                     /locus_tag="ACG8Z3_000453"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0822801.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(61187..62659)
                     /locus_tag="ACG8Z3_000454"
     CDS             complement(61187..62659)
                     /locus_tag="ACG8Z3_000454"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0822802.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(63296..63658)
                     /locus_tag="ACG8Z3_000455"
     CDS             complement(63296..63658)
                     /locus_tag="ACG8Z3_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0822803.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63881..64633
                     /locus_tag="ACG8Z3_000456"
     CDS             63881..64633
                     /locus_tag="ACG8Z3_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0822804.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64773..65696)
                     /locus_tag="ACG8Z3_000457"
     CDS             complement(64773..65696)
                     /locus_tag="ACG8Z3_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0822805.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65711..65983)
                     /locus_tag="ACG8Z3_000458"
     CDS             complement(65711..65983)
                     /locus_tag="ACG8Z3_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822806.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        19149 a        13543 c        11484 g        21979 t
ORIGIN      
        1 atactagctg tttttgtgtt aattaaagga taaaatacaa tataaagaaa catctgtttc
       61 tttatttaga ataaaaaaat acagactctg ttcaaataaa tgaacctagc ctgcattatg
      121 agattgacta tactttttaa ttcattgaga tgaataactc gtttttataa atgcccaaaa
      181 gtaatcggtg ccgaagccat tcctcgacct aattcttttt caaattttcc tattgttgat
      241 atctccatat aaaacggtga tactggttta gacaattctc ccctgattgc taacggacgt
      301 aaactacgtt ccaaattatt tatgacttta ggataatact gacctttttc taagcctgtc
      361 tttgcgtcta tcaaaagtat acgttccttt tctttgatct ctggatttaa aacgagatca
      421 tataatttta gtaccatgtt ttctatttct tcacttttag acatcacgat ttcctcccaa
      481 aaatatattt cttcccacta cattctataa acaaagtata cgctacatct agattttttt
      541 aaatcattac tggattgtta aaacctttca atacactgtt atggcaacga tttttatttt
      601 aaaaacattc taaattaaat cagtaaaaat ttttattcta agatttttat tagaattacg
      661 cctaatttgt gtctctcagg gcaattctaa gaacgtttca agagatagcg acactatcgt
      721 tatcgaaaaa ggcgaaaacc tatctcattt tctgctaatg taagttggtt tagcttttgt
      781 taatatgact ttatgaaaat ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc
      841 aagcttgaat aaaggatcct tttttcatat ctatgaaaac gaagattttg aactgtataa
      901 tatcctttca caaaaagagg tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa
      961 atctattttc aagtatgcat tttttgcttc caatgatttt caaagaatgg agattaccga
     1021 ctgtatattc gaaaactgtg atttttcagg ctgtatattg acaaaaagca tgttgcatcg
     1081 cgtgaaattt gtcaattgta aattaacagg tactaaattg atggaaagtt atattgggaa
     1141 cacgttattc gagaattgta aaatggacta tgtaaattta tctggttcaa acataaaaga
     1201 aagtaccttt gagcactctg tcttatcaag cgcagatttt gtagactgtt ccctcactaa
     1261 aataatgttt tctactaatg atttagaaaa tgctagcttt tttgatactg atttaaaaaa
     1321 tatcgattta agtagcaact cctttgaaat gattgaagta cgctcggaac atctaaaaaa
     1381 ttgtacgctt aatcagttgc aagcacttgg ttttgctaga aaatttttac aaataaaagt
     1441 agtataatca aaatgaatat ataagataaa tttttagcaa tacaaatatc cataaaaaaa
     1501 cattgcctat tttaactctt catgtatact gactctatct tcaaaaactg aatagtagga
     1561 gttattatca tgaataacat tttaggaaaa aaaatagaac ttaaaaaagg gatattgggc
     1621 actattgtcg ttgtactcgg tttgttaggg ttgctatttg cgccatccat tattagcatt
     1681 ttttctttaa ctatcactgg atttatagca tttacacttg tttttggtaa aaaagatacg
     1741 aaaaaaatgt tctcgaaacc tgtcgctcct gtcaaaaata tagccaaata tttttttatc
     1801 aatgtagtaa tcagtgccgc agtctcggta tttttgcaac aaattttgaa atggggttta
     1861 acaggcaatc caataaatga ggctttcagc ccgttattat ttattatttt gcctatcatg
     1921 atacttggag aagaattgtt ctcagtgtat tttctcgcta ttttttctag taagttcagc
     1981 attcctgtcg ctagtttctt gtcagcaatc atctttggat ttatccatta cagtacttat
     2041 gataatggaa atattttaca cactgtagca cacatccttt tgatacaagg ggtggcaaga
     2101 ttgttattca accaagctgc cattaaaagc aactcgatca ttacaagttg ggcagttcat
     2161 gtgatatttg atttcacagc tatattgcta gtcgttttat tctcctaaat ttttatagaa
     2221 ataatacatg ctctattgct aagaaagcat actttatttc ctttaaacaa aaagacagat
     2281 gtatcaatgg attaaaaatc cttgatatat ctgtcttctc ttttaatgcc agctataggg
     2341 atcgaaccta cgacctacgc gttacgagtg cgttgctcta ccaactgagc taagctggcc
     2401 ttatccaaaa gaaattatat actctatttg gctgattgta aagagtccga tggaaaatta
     2461 gtttaatttc atgatatttt tcataaaacg tggtataatt gatgtgaaag gcaggtttta
     2521 tgaaaatggc aacttttgga aaatttgata caactatcgt accatcggaa attggtgaaa
     2581 aattcaagat caatactcac gtaatgttca caattcacgg caagtcgcaa actggaacga
     2641 tttcaaagca attgaaaaat tccgctgttg ttgagattga tgaaactgca gaaaatgcga
     2701 acttgatgac gcaaagtaag ggtgtagtaa tcgttaacta caaagaacta aaaaagattt
     2761 gaatttgacc atctctgcat ttttgggatg gtttttttat atgcaaacga aagctgtgaa
     2821 aaagctgttc tgcatcaaac gttaaaaatg accaaaaaag gatgtgacaa aagttgtgtc
     2881 acaccctttt tcgtatttac ttattttgta ccaaacaaac ggtcacccgc atcccctagt
     2941 ccaggaacga tataaccatg ttcattcaat ttttcatcta gagaagctgt atagatgtca
     3001 atatcaggat gcgcttcttg caaagctttt actccctcag gtgctgctac caaacaaaca
     3061 aatttaatgt tcgacgcgcc acgttctttc aatgcatcga ttgccatgat tgctgatccg
     3121 cctgttgcca acattggatc tactacaaac aactgacgtg catcaatgtc ttctggaagt
     3181 ttgacgaaat attcatgtgg ttccaatgtt tcttcatctc gataaagacc gatatgtccc
     3241 actttagcag ctggaatcaa ttccaagata ccgtcaacca ttccaatacc tgcgcgcaag
     3301 attggaataa tcgctacttt tttaccagac aacgtttttt gtgttgtttc agtgatcggt
     3361 gtttcgatca cgatgtcttc caatggcata tcccgtgata cttcatatgc catcaacata
     3421 gcgatttcat cgactacttc acgaaaaacc tttgttccgc agtttttgtc ccgaataata
     3481 gttagtttgt gttgaatcaa tggatgatcg atcacttgaa atttgcccat tttttaagct
     3541 ctcctttgtt ctgtcgtttc tttttaattg taaaacaaaa tcaagtgatt gactagtcaa
     3601 actcgccatt tttcctaaga aaatccgttt ttgaaaagaa gttttcctgc ggacaagcaa
     3661 agcttattct tttttcttga tccaagaagt agataatgcc attgctgcac gcgttgcctt
     3721 tgtctgatcc atagcattta ccatgacaaa atcatggatg attccttgga agcgtacttg
     3781 tgtaacagga acccctgcat cacgaagttt gcgcgcataa gcctcacctt catctcttaa
     3841 tacatccgct tctcccgtta aaatcattgc tgaaggaaga tcatttagtt catttatatt
     3901 tgctctcaaa ggcgaagctg tgatttcagc acgctcttct tcattcgtcg tatattggtc
     3961 ccaaaaccac atcattcctt ctcttgtcaa aaaatagttt tctccgaatt catgatagga
     4021 atctgtatca aaatcagcgt ttgtgactgg ataatacagc aattgcttgg atattgggag
     4081 gccctttcgt tcttttgtta agatcgtcat gactgtagcc atgttaccac ctactgaatc
     4141 tcctgcaacg attagattct cgagatccag attttttgac ttggctaatt tattcagctg
     4201 ttgcaacaca gcataatttt gttcaattgc tgttggatat tttgcttctg gtgatcgact
     4261 atattctgga aagacaacga cggaattcgt acgaaccgca agttctctga tcaatttatc
     4321 atgggtttga gcactgccaa aaacccagcc tgctccatga atataaaaaa taactggtaa
     4381 tttatccata ttcccttctg gtcgtacgag acgaatattg agttttcccc attcgccagt
     4441 atccaccatc aagtcttcaa tatcgacgga atatttgttc acaggtgatt cttgtacttc
     4501 ttctagtaaa actcttcctt cttccggtgc tagttcatag atacgcggat gaggcgcatt
     4561 tgcatcactg aatgctactg cttccttttc taacgagata cgatcagtca tgacttttcc
     4621 tccctgagat ttagtgtgaa gactaaaatt acacctcata tactagagag catcaccgat
     4681 attgtcaaag aatccgtttt cccaaaaaat aaacagaaaa gcccaacatt gaacttttct
     4741 gtttactaat ggaatttaaa tttttatttt ttatgcaacg gaaatttttc agttaattcg
     4801 cgtacacctg ttttgacttc gtctagttga gcgttatcat cttttgcttg taatgctttg
     4861 acaactagtt cagccacttt tacagcatct tcttctttga acccgcgagt agtgattgct
     4921 ggcgtgccga ttcgaatacc gcttgttttg aatggactta gtgtttcaaa aggaatcgag
     4981 tttttattta cagtgatatt cacactgtcc aaaatgctct ctgcttcttt tccatttata
     5041 cctaactcgc gaacatcgat caacatcaga tgattatctg tcgcccctga aatgactctg
     5101 gttccaattg cttgattgaa aactttgacc attgcttttg cattggcgat gatttgttca
     5161 ctatattctt taaatgcagg atctaatgct tctttgaatg ctactgcttt acccgcaatc
     5221 acatgctcta gagggccgcc ttgaatacca ggaaataccg cactatttat tttttttgct
     5281 aatgcttcat cgtttgtcaa aatcatgccg cctcgagggc cacgcaatgt tttatgagtg
     5341 gtagtcgttg tgatatctgc ataaggaact ggattaggat gaagtcctgc tgctactaat
     5401 cctgcgatat gtgccatatc taccatcagt tttgcgccta cttcatctgc gatttctctg
     5461 aatttcgcaa agtcgatcgt tcgtccgtaa gcactggcac ctgctacgat caattttggt
     5521 tgatgttttc tagctaaaat acgtaccaca ttataatcaa taacttctgt tgtcggatca
     5581 acaccgtaag caacaaagtg ataggttttg ccactgaagt tgactggtga tccatgtgtt
     5641 agatgtccgc ctgcggaaag atccattcct aagatcgtgt cccctggttc taccaaagcg
     5701 agataagcag ccgtgttggc ttgagagccg gagtgaggct ggacgttcgc aaatttcgct
     5761 ccgaataatt cttttgcacg gtcaatcgct aaattttcaa cgatatcgac aaattcacac
     5821 ccaccataat agcgatgccc tggataacct tctgcgtatt tatttgtcaa gatacttcct
     5881 tgtgcagcca tcactgcttc ggagacaaag ttttcagagg caatcagctc caaattatgt
     5941 tcttgtcttt cttcttcttt ggcgatcgca gcccaaagat cagggtcaaa cgttttgtaa
     6001 tctaccacaa acaacagctc ctttttattt tcctacaatt ttatttttcc catcaaaaac
     6061 aaattgccaa atattcgtat ctcttctatt acgattagtt acgcttagtt tttcgttcac
     6121 tgatagaaga tttacttgat agcattgtat catactaaaa gaatgcgaca ataaccaatt
     6181 gatcattttt caaaataatt ttgtcctgct gcttttttca agcgattcat gtaagcattc
     6241 cccaatccag tttctggata cacttgtaca aaaatcaaat ctaatccaag tgttggttca
     6301 tctaatcctc ttagacctgc aaacaatcct ttagccgccg cttctactga gttgtcgtta
     6361 tacatataaa cagctgcagt atctgtccgc acttgatccg caatttcggg gcttgctatg
     6421 acacctgcac ggatcttctt attttttgcc cattgaactg cagttgacca atcgccatct
     6481 ctgaccatca gaacacgtgt atcgggtgag tagtgtttgt atttcatccc aggggctttt
     6541 ggtgtttcat tttcttttac aagatgctga tcaatagcaa caggagattc gatgactgct
     6601 tcgatttgtt ctttcgttac tgcacctggt cgtaaaatca taggcatggc tgttggatca
     6661 cttaaatcaa gaacagtgga ttctacacca attcgcgtag ctccatcatc aataatcccg
     6721 gtaatttttc cttgtagatc atgatataca tgatctgcag tcgttggact tggttttccc
     6781 gacgtatttg cacttggacc gacaagcgga acaccggaaa gttcgattac ttctagtgtt
     6841 tttttgttat ccggtatacg aaatgcagct gtggataatc ctcccgtcac aactgagggc
     6901 aaagtgtctt ttttgatctt aaatatcaaa gttaatggtc caggccagaa gttcttcaca
     6961 atcttctctg ctgccggatg aaagttgtcc acatactctt ttacttgttc aaaagaggcg
     7021 acatggacga ttaatggatt atcttgcggt ctccctttta cagaaaacac tttcttgaca
     7081 gcgtttggta aaagagcgtt ggcgcccaat ccgtatactg tttcggtagg aaaagcaaca
     7141 agttctcctt ctttcagtgc ttcggctgcc tcttgcaatt ctgtttcatc atatcgtttc
     7201 gtttccacag tttatccaca accccttttt catacattct tattttgtct gagtttatcc
     7261 tcaagttttc cactgtttgt ggataatcta tttgtcaata gtggataaac ttgttgataa
     7321 accaaaaagt cgtttacatc cttgtcaaat aagtgttttt ttataataaa aaaacaaata
     7381 aaaaattttt catccacaac ttcaaccata aacttggatc attcgttcat tgccaaaaag
     7441 atcctttttt atcgccactt ttttatctgg aaatgcttgc tgaaatattt tttttactgc
     7501 ttcaccttgg cgaaatccga tttctaaaaa aatctttcca tttgatttta gtacgcttgg
     7561 tgcttcaact gctatttttt cataaatagc taatccatcg ttctcagcaa aaagggccat
     7621 tttaggttcg aatgaccgga cactttcatc catcagttcc cattcattcc gactgatgta
     7681 cggcggatta gaaataatca catcaaattg ctgatccatt actggtccta atgtatctcc
     7741 atgataaaaa ctgattttcg ttccttcttt ctgagcattt tctttcgcta cttctaatgc
     7801 ttcttctgaa agatccacag cagatatatg ccaatttttt cttgctgctt tcaaactaat
     7861 agcaattgct ccagtccctg tcccaatatc tatcacttct aacggtacac caggcgtctc
     7921 gtctaaacac cattcgacta actcctctgt ttctggtcga gggatcagtg ttgcttcagt
     7981 tacctttaac cgatgatcat aaaaatctgc atatcctagc aaatactgag gtggatgatc
     8041 tgcaagcaga agtcgcagat cggtttcgat caaccgctgt tcttcttctg aaatctcttc
     8101 gttcatatga aggagccaat caagtttttt ccattgtttt cgttccaaaa ataaaaattg
     8161 gatactatat gcttctttcc catgatcctc taaaaaagaa gaagcccgtg acaggacttc
     8221 tcgataggtc attttaccca ttttgcatag cttccagttt tgatgtttga tcatacatga
     8281 tcaatgcatc aacgatctca tctaatttcc ctgctaaaat ctgatctaat ttttggatag
     8341 ttaatccgat acgatgatca gtcacacgat tttgcgggaa attatacgtc cggatccgtt
     8401 ctgaacgatc cccagttccg actgctgatt ttcgattggc atcatactcg ctttgtgctt
     8461 cttgctggat cttatcgtac acacgagcac gcaagatttt cattgctttt tcacggttct
     8521 tgatttgaga acgttcatct tgcatagcaa caacgatccc tgttggtaag tgagtcaaac
     8581 gtacagcaga tgctgtttta ttgacgtgct gtccgccagc acctgaagca tgatagatat
     8641 ctgtacggat atctttgtct tcaatatcga tctctacttc ttcagcttct ggcatcacga
     8701 caactgttgc ggtggaagta tggatacgcc cttgcgattc agttgaagga acacgctgga
     8761 cgcggtgcgc accactttca tattttaatt tcgaatatac gttgtctccg gtgatcatca
     8821 tgatcacttc tttatatcca ccgattccgg taacgttagc ttccatcact tctgttttcc
     8881 agccttgtgc ttcagcatac ttttggtaca tattgaaaag atcgccagcg aataatgctg
     8941 cttcgtctcc gcctgctgct ccacggattt ccatgatgat atttttatcg tcatttggat
     9001 cttttggtaa aagcaagatc ttgatttttt cttctaagac ttctttttct ttttttaatt
     9061 cagataattc ttcttttgct agttcagcca tttcatcatc cagtttttca ccaagcagtt
     9121 cttctgtatc ggtgattcct tgtactactt ctttataacg acgataaact tcgactgttt
     9181 cgcgcgtgtt tgcttcttct tttgaaagct gcataaaacg ctttgtatca gaaatcactt
     9241 ctgggtcact taaaagttct ccaagctctt cataacggtc ttcaattgct tgtaattgat
     9301 cgtacatgct tattctcctt ctgtaatcat tgatgggtga aagtaatgtt ttcgacaaac
     9361 ggggtaataa gcttcatttc cgccaatttg cacttgatcg ccttcataaa cagggtttcc
     9421 atcaatataa tgtaaattca tgattgcttt tttgtggcag aaccaacaga ttgttttcat
     9481 ttcttcgatt ttgtctgcat aaagcaataa atattttgat ccttcaaata actcgtttcg
     9541 aaaatcattt ttcaaaccaa atgccataac cggaatatcc aattcgtcca caattctcgt
     9601 aaaagcaatg acatgtttct tttttaagaa ttgcgcttca tcgatcagca cacaataggg
     9661 tttgctttcc attttctgaa ttacgtcaaa tacgtctgtt tcttcaaata tcggcatcgc
     9721 attacgtttc agtccgattc ggcttgaaac aacgcctaaa ccatctcttg tatcaatacc
     9781 gcttgtcatt aagacaactg gcttattttg ttcttcataa ttatgtgcga cttttaggat
     9841 ctcgatcgtc ttgccgctat tcattgcgcc atacttaaaa aatagttgtg ccatgctctt
     9901 tttcaccttc cagtttgttc ctatggtatt atacttgatt ttcagcattt tttacagtga
     9961 gaattttcat caagcacaga ttagtattaa atctattgct cacttttgtt ccaatttttt
    10021 gacaagaact aaacatgggt tccattcatc ccataaattt ggaaaaactt ccaaccttga
    10081 aaatcctaat gattcgtaga aacaaatagt ttgatcatat atagagtagt gcccctcgtc
    10141 tacagtcttc acttgcaaat atttgtaatg ttttgctgcg taaccttctg cttctttaaa
    10201 aagttttgtt cctaatcctt ggtgatgata gtcttttttt attcccatac aatagaatag
    10261 atttcggcag tatcttcact tgtctgtgaa agagtgataa agcctaaata ttcttctttt
    10321 cttttttcac accataaagg tagtttactt gattcctcga tatatttttc cgttgattca
    10381 ggaagcccaa accactcagg caagtctagt aatatttctc ggacacactt tcctttttcc
    10441 tctctatctt ttacaaacga tatcggcata ttctttctag attctccctt tttgaattga
    10501 gtatagcatg ctttctttct tttcacgtgc tataataagt taaaatttca aagttttacg
    10561 taaggagcta tttgatgaca aaaaatattt ttgattccat cgctaaccat tatgattccc
    10621 ctgaccgtca agcattagcc aagattattc gcgaagaact aactacttat ctaccgcgcg
    10681 acagccacca aaaagtcctg ctggattacg gaggcggaac tgggcttgtt agtttaccgc
    10741 ttgcggaaag attcaaagaa ctgatcattg ccgatgcttc agaaactatg ctgaaaatgg
    10801 cagaagaaaa gatccaagca gcagatctga aaaatgtccg aacgatccac gctgatgctt
    10861 ctgtcgaatt tcctgctgtc caagctaatc tcatccttct atcacttgta cttttacaca
    10921 ttcctgatac ggagaatatt cttacaaaac tttatgaaat tttagctcct ggcggtcaat
    10981 tgatcatcgt agactttgac aagaatgaac aaattagtca tccgaaagtc cataatggct
    11041 ttagccaaga agaattgaat aatcgattgg aaaaaacggg ctttgtctct gctactagtc
    11101 ataccttcca tcgtggggaa aagctattta tgaacaaaaa cgcttcgctt ttcttatcca
    11161 tcagccaaaa agcttaaaat gagattgcaa aaaaagtgag caactccaag aaataagaag
    11221 gaattttcgg aaaaagaagc tgtgacaaaa aacatgtcac agcttctttt tattttttca
    11281 tcacatgatt ttaatttctg aaagaggcca ataacggaaa acaacttttc cttggatcat
    11341 atctttgtcg actaacccaa acattcggct gtcttttgaa atcaagcggt tatcgcctag
    11401 aacaagatac tttccttttg gcacagtata ttcaaagtca ctcgtaaagg tggatgaact
    11461 ttctgcttgt gcttggaatc ctgagctata agcatattct ccttgtaact tatcttctga
    11521 aaattctttt ttgaactcat ccagataagg ttcatcatac tttttgccat taatggtcaa
    11581 ctggtcattt tccatcttga ctgtatctcc aggcataccg atgatccgtt taacaatcat
    11641 ccgttcttca tcccccggtt cctttgtagt aataatatcc attcgttcat agtttgagat
    11701 cttagatgaa atcaatcgtt gtccatcact taatgtaggg tccatcgaat gcccgtttac
    11761 agtaactggt gtaaacacgt acactctagc taataaaata gccgctaata caaggatcaa
    11821 aatcccccaa tttttcaaaa attctttcac agcttacctc ctacttgtat tttcctcgtt
    11881 atttattacg ttttttcaca atacttcgtt gtactttctt tattctacaa tgtttataaa
    11941 tgaaaggaaa gaataagtga tgtgttcgtc ttatctttct cgttatataa aaaaggaacg
    12001 tgacaaaaga cgtgtcacat cccccttatt ccgaataaac gtttttatcg caaccactga
    12061 tacacggcgt ttcatcacat tatgaatgtc ttcttctttg gctacgacta taaaccactg
    12121 gttcttcctg cgactgtgcc ttttttgcaa tcaatttcag acgataaggc tcatgatagc
    12181 gtaacccgtc tttgacaaaa tcaccgccga cgatgaacaa tccgacaggt tccaatgtgt
    12241 cttctgttga gtcaagtcga atggtcccta ctttttccat gtccgagtag cttcgtccaa
    12301 tatttagaac atactctttc tctcctgttt tcttgacata gtaataagga tacaatgtat
    12361 ttctatcgac cagaaatcca tccatgcgac cgagttcatt tgttttgatc tcaccatttc
    12421 caatcaaata ccgcatattc tcggctaatt tcaattgata atcttcgtca taagaaacca
    12481 tgacctcttg tgtattcact ttatcgatcg aatttccatt atctatcgat tcttctgtct
    12541 ggatgttgaa cgcttcatca ggtaaatact tcacaaaaag ctgagtgaca tcggctttcg
    12601 tgtttttatc taaaaagtaa taaaatacat ttaaaacagt aaaataaaca agcactagaa
    12661 atgcaagtcc atataagaaa aattgaagat agttttggtt ttgccaaagt ccataaacaa
    12721 ttctcaataa ataaagcgtg ggaatcacac ttaaaatcgt atatccacgg ttaagatatt
    12781 taggactgat gtctaaatag ccaaggaatt tatgcacccc gttgataagg tctagtaaaa
    12841 atgtcattga gaatcacctg cttgctggtt atacgtttgg gtatttcctg aatcttggtt
    12901 ttgctgtgct gtacgtgctg catcatcatt ttgcgtgtta tcatctacag tcgtactgct
    12961 tggtatcgtt tcggagctag cacttgtatc cggacttgta cttggattgg ttgaatccga
    13021 tggagtgcca tctggtaccg tattacttgt ttcgcttgag ctgtcagttg tatatgtctc
    13081 atcatacgac gaactagttg ttgtatcgtc ccatgtactt gtcgtagtgg tttcattcaa
    13141 actttccgtt gttgttggtt cagatgtcga gactgaagta gttggtgttg tttcttctac
    13201 agtactagaa cttgaacttg gtgcacggta gctggaacta ttcacaatac ctgtagtagt
    13261 cgcgacaatc gtcgagattg ttaatacagc gattggcttc aaaataggtg ctacttttcg
    13321 catcttatct cttccttcat tgaaatttat tcttgtttcc ttactttact acggacaaat
    13381 tatacagtct accttatttc taccataaat tctttatttt tttcttacgt ttttccgaaa
    13441 gatttcttta attttcctca aaaactacgg ttgatgtttt taagtaagct tttgcttccc
    13501 gattgatttc tttcagatcg catccttgct gcttggcagc gaacacgcaa ccacaataac
    13561 attggcgata aatatcatac tctttgcaca tttgcaaaga gcgttcatag cctttgtttt
    13621 tcttgaaatc acttggcaag tattgtgtcc cgtaaatctt ttggatatcc attccgatct
    13681 gattgatcaa agctgcattt ttctttggcg agatggtcag ggcacttcca aagtaatcgt
    13741 atcctagttc ttgcgctttt tgcgcaacaa gatctaatcg catattgaag catgcactgc
    13801 agcgagcacc accttctttt tcgttttcaa gatggtttgt taaaaccatt ttggtgaatt
    13861 catttggacg gtattcatca acgattagcg caacatgatg gtcagttttc tcattaaatt
    13921 ggtctacaaa tcgtttttgt tcatgtaatc ttcgcaaata ctcactttta ggatgaatat
    13981 tcgaatttga aaaaaataac gtgatctctg catattgaga catgaattcc aatgtgtgcg
    14041 tactgcaagg agcacagcat gtatgtatta atatcttagg ctttacttct tctttctccc
    14101 attgcatgat catttttttc agaactgtat cataatttat tttttgattt ttcatttttt
    14161 ctacgatttg ttgagcatta atcaatttgt tattcctcct ccttcttttt tctaggattt
    14221 tccttattat aagccatttc tattttaatc gaaagctgta ttgttatctg tttgataatt
    14281 tctttgagta ttcctaattc cccttgtata ctggtagaaa cgactattgt caggaggata
    14341 gtactgtgca tgttgattta ttctttcgaa ggctgcagcg gcgaggacag aagtttgctg
    14401 ccaatcattt ctcatcgatc caaatcatcg ttttttatta tatattgatg acggtcttat
    14461 cactcgttct attttatatg ccgatttttc gcgaaccaga tagccatgtt tcttttgtgg
    14521 acatgctgtt catggcaatc agtacagtga gtgtgactgg actatcgact tttgatatca
    14581 attctgtgtt caatgataac ggtattatac tgctggaaat attatttcaa gttggtggtt
    14641 tggggatcat gatgatttct actgcttttg tcattttttc taaacgacgg atcacactga
    14701 aacagcgaca gctgatcatg acggatatga atcaaccgcg tttgtctggt atcgtccgac
    14761 tgattcggat cacttttgct attttaatct ggtttcaatt actttttggg acattctttt
    14821 ctatttattt ttattacaga ggctattttg atcgttggcg tgatgccgtt ttttacggtt
    14881 tttatcaggc gatatcggct gtcacgaact ctgggtttga cgtcactggg gattccatca
    14941 agccctttgc acatgactat tttttcttga tcctgatcat gtttttgatt tttgtcggag
    15001 gaattggttt tcctgtattg atggagtgcc gggaatggct attatacaaa cgttccaatg
    15061 cgaagcttcc gtttcgtttt tccttgttta ccaaattagc tgtgttagct tttgttatct
    15121 tgtttgtttc gggaactgtc ttgatttatc tattggaaaa ggaccattta ttccaagatt
    15181 ccaatatgag tgtcaaatgg atcaattcga tgttttattc gattacgact cgtaatgctg
    15241 gtctgcagat tcacgatttg ggtgattttc aaatcacaac actgatcatt ttttctttac
    15301 tgatgtttat cggatgcagt cctagttctg taggcggcgg gattcgaaca actactgtag
    15361 caatcatcgg tttgtatcta tattccttcc tcaaaagtga agataacatc aatatttttg
    15421 gacggagaat cgatcaagat gatgtgcgaa aatcagtcgt tgtctttatg ctttctcttg
    15481 gtatgtgttt cttttgtatc gtttttttat ctgctacaga agaacaaacg ctgatctcga
    15541 tcatcatcga agtaacttct gcgtttggaa caactggttt atctttaggg attaccggag
    15601 acttgtctgt tgtgggcaaa atcacgattg ctacactgat gtttattggg cggatcggaa
    15661 tgctatatac attgatgtta ttcgtgccaa aagaaacacg agatctaggc tatgaatatc
    15721 caagcgagaa aattattatc ggttaaaatg ggaatatagg taagagggaa taataaaatc
    15781 acagcagctt gaaacaatca ttttgtttca agctgttttt ttgttaaatc atgcgatttt
    15841 gtccgatttc atgcactttc agccgtaacc ttttcttaag aataagaaaa gcaatttaac
    15901 ctttttttca acatatggta tgattagttg aatttattcg tcagcaaatc gtttttggag
    15961 gttacataat gggaatacgc agtcattttg cgattgcagt tggaaaaacg tcgcaatggg
    16021 cattaaaaac tttttttaaa ggcggatcaa gtttaccagg taaactagcg ctgaaagtcg
    16081 atccacatat actagatagt ttggcaaaag actaccaagt cgtcgttgtc accggaacaa
    16141 acggtaaaac attgactacc gctttaacag tcaatatttt gcgccagcaa ttcgatgaag
    16201 ttctaaccaa ccctactggt gcgaatatgg tacagggaat tgtatcgact ttcttgcagg
    16261 caaaaccaaa aaaaggacag aaaaagtttg ctgtcctaga aatagatgaa gcaagtttga
    16321 gcaaagtcac agaatatatg aaaccagaac tgttcttatt tacaaatatt ttccgtgatc
    16381 aaatggatcg gtatggagaa atctatacga cctataaaat gattgtagac ggtgccgcaa
    16441 agtcaccaaa tgcaacgatt atcagtaacg gtgattcacc aatcttcaat tctatcgaaa
    16501 cagtcaatcc tagaaaatat tatggctttg accatgaaga agatcgtgaa caaatggctc
    16561 attacaatac agacggtgtc ttatgtccaa attgtcatca catcttgcat tataaaatga
    16621 tcacttatgc aaacttaggg aaatattact gtccaaattg tgacttcaaa cgtcctgaat
    16681 tggattatcg attaactgat atggtacgga tggacaatgt gtcagcagat ttcgtgatcg
    16741 atggtcatga atacggaatc gaagttggcg gtatgtataa tgtctacaat gctttagcag
    16801 ctactgcagt ggcagagtac ttcaatgtcg atcctgataa aatcaaacaa ggtttaggat
    16861 atgacgagaa agtattcggc cgacaagaag tcatcaatat caatgggaaa aaatgtacat
    16921 tagtacttgt caaaaatcct gtaggtatca atcaagtaat cgatatgatt ggtctagctc
    16981 cttatccatt ttctttagtg gctttattaa atgccaatta cgcagacgga atcgatgtca
    17041 gctggatttg ggatggtaat tttgaaaact ttgccgatat ggatatccca gctgttattt
    17101 ctggaggaga ccgtcataca gatatgactt tacgtctaaa agttgccggg attccagaaa
    17161 ataaattgac agaaacaaaa gaattaccag aagtaatcga aaaaatcaaa gaattgccaa
    17221 ctgagcatgt ctatatttta gctacttaca ctgctgtatt gcagcttcgt aaagaactag
    17281 ctgctcaagg atttatcaaa ggaggaatga accgtggcta attatgaact caatatcgcc
    17341 cacctttatg gaaatttgat gaacacatat ggtgataacg gaaacctttt gatgttgaaa
    17401 tatatagcag aaaaaatggg ggtttcttgc catacggaga tcgttagtat acatgaacct
    17461 tttgatgctg ataaatatga cttggttttc tttggcggtg gtcaagactt tgagcaatta
    17521 atcatttcaa aagacatcca gaaaaagaaa gattctttga tcaactatat cgaaaatgat
    17581 ggcgtcatgt tagccatttg tggtggctac caattgctag gtcactacta catgggagct
    17641 aatggagaaa agatccaagg aatcagtgca ctggatcact acacgttaag tcaggaaaat
    17701 aaccggttta tcggtgatat cgttatccat aacgaagaat tcaatgaaac ttattatgga
    17761 tttgaaaacc acaatggtcg gactttctta ggtgaaggtg aacgaccttt ggggaaagtg
    17821 gtacaaggtc aaggaaataa tggtgaagac caatctgaag gtgtcattta ccgaaatgtc
    17881 ttcggttctt atttccatgg acctatcctt gcaagaaatg agaacctggc agctcgtttg
    17941 atccgcctag ctttagaaca acgttacggt aaagaattgg atctgcccta tcttgaagga
    18001 caaacagcct agttattcga aaaaggggaa tgtgacaaaa atttcgtcac attccccttt
    18061 ttcgtatcct attttaagcg acttgcttgg cataacggtt cacgttttgc ttgatattcc
    18121 cgattgtttt cggatcattg attcctgtga cttcttgaat caattgttcc aagttttcat
    18181 ggatcttcat ttcatgcaat tgacggcttt gttcatcttc aggatcatag taattaaaaa
    18241 taatccccat tgttgccgtc aaatgtgggc aagtcaagtt atgttcttgt agttctctca
    18301 ctggacgtgt aaatctttct tgataaccta acttacgtaa tggtgtccga gcgacacgtg
    18361 tgatcgcatc agagatattt ttgttttgga agcgatgaat gattttttca atatacgcat
    18421 gatgctgctc tgcatcaaac ccccatttgg caatcagcaa acttcctgtt tcctgcaaaa
    18481 ctgattttaa ttgtgccaca actaacgcat cttgcattgc ttcatcaatg gtctggtaac
    18541 ctaacaaggc acctgtataa gcgacagtcg catgaccagt attgacacta aatagtttgc
    18601 gttcgatata tggttctaga tcatctacat ataaaacccc ttttaattgg atttttttat
    18661 tttttctttg gctatcgtca atgacccatt ctttgaaagg ttccacttgg acgaataaag
    18721 gatcttcatg gtgttgtaat gggacgattc gatcgactgc tgcatttgga aagccgatat
    18781 atttgtcagt aaatgatgtt tccggaagat atttatacac ttctttttct agaaatgctg
    18841 aaccgccaat catattttca caggcaatga tatctaaaga tgttgtgctt ccttcctttt
    18901 ctctttcttg gatacctttt gcaatcaact cagcaataaa aggtaagata ttcgggccaa
    18961 tggcggtagt aactaaatct gcttcagcga tttcttggat tactttttct ggatttgttt
    19021 gattgttgat tcctttgaca ttttttacag tgattttttc ttttgattca tctgctaatt
    19081 cgatttcgta ttcttttctt tcatttaaag catcaatgat cgtgtgattc acatctacga
    19141 aagtaatctc aaatccatta gctgccaaaa cctctccaat aaatcctcga ccgatatttc
    19201 ctgcaccaaa atgtactgct ctcatttttc tctctcctta agcaattgct agattttccg
    19261 ttacttcttc tttgctgagc gcgtctgcta gctggacaac attgtccata tcactgcaat
    19321 aaagtgcgat ttgctgtaca agttgcaaat gctcatctcc gactcctgca ataccgaaca
    19381 aaacagtcgc aatcttttct tcttcttcgg taccaaaatc tacaccatct ggtacttgaa
    19441 cgacacagat gccggatttt ttcacatatt tttttgcttc atctgttcca tgaggaatcg
    19501 cgataaaatt ccccatataa acagataaca ttttgtctct atctaccatg gcatcgatat
    19561 attcctcatc aacgcaccct gcttctacaa gcatctgacc gcaatagcgg atcgcttctt
    19621 ctttggtttt aaaagatcga ttgagctcga tcatgtcgaa tggcaaactt tgcatgctgt
    19681 tttcctccct taagctgtct gtttcaaata ttgtgcaatc gattcgtatt tttgcggatt
    19741 gaccatgctg ttgactgata aatgaatgga ttgaggtgca gccatctgta ctttttccgc
    19801 taaactttct ttggctacga tcagtgaatc aggttcatct tttaaatctt ggatattgac
    19861 ttttcttaac gggatagtta aatggttctt gtctaatata tctttgagca cggtcatccc
    19921 cattgttgct gatccggaac gatcatcgtg gacaagtaga atctctttga tttgtttgat
    19981 gtttgctgaa agaaactctt ctgctggctc attctcaatt tcagaaactg atgttttctg
    20041 cttgctgctt aattcttgaa tgatttcatc atatcgaggt gaattcaaaa agttttctac
    20101 tgagacaaaa cgcgcgctcg gtgctttttg ttttgctctt tcttgtagtt cgacctgagt
    20161 aacgatcaat gtattcgagt catctgtcag ttggctgatt gcttgattgg tcaccgtttg
    20221 aggcaagtct gcttcctgta ctttttttct caagatcgaa gcgcccatcg cacttgatcc
    20281 cattcctgca tcacaagcaa aaatgatttt ttgtacgcca gagaatgtag ctccttgagg
    20341 aataccttgg gcacctttgg aagcttgctt agctgccttt gtctcttgaa cagatttttc
    20401 gaagtcttca tttgtatctt tcgcatccgc tttcaaaatt actgcagaaa taccaaacga
    20461 aacagctgcg gcaacaatca cgcccaaaat aacaggaagg taagaattga acggtgtcat
    20521 cgctagaatc gcaatgatcg accctggaga agctggcgct cttagtccag catccatcaa
    20581 ttggaaaatg aaacttcctg acattcctcc gcctataact gataagaata ataacggttt
    20641 catcataacg taaggaaaat agatttcatg gatcccgcct aaaaagtgaa tgatcatggc
    20701 tcctggtgct gaagattttg ctgctccttt gccaaacaaa gtaaaagcaa gtaatacgcc
    20761 aagtcctgga ccaggatttg cttcaagcaa gaatagaata gattttccag cctctgctac
    20821 ttgttcagtt cccaatggtg tcaaaatccc atgattgatt gcattattca aaaataagat
    20881 ttttgctggt tcaataaaaa tacttgtcaa tggaatcaaa tgggcagata aaatgatctc
    20941 tacacctttt gccattgctt gagtcaatgc atcgacaact ggaccaatcg cagaaaaacc
    21001 taataacgct aatccaaaac cgataagacc tgctgaaaaa ttattgacta gcatttcaaa
    21061 accggatttg atccgttttt ggaatagctg atcaaatttc ttgatggcat atccaccaag
    21121 aggtcccatg atcatcgcgc ctagcatcat tggaatatct gttccgacaa tcacacccat
    21181 cgttgcaatt gccccaacaa ctgatccgcg gtcaccagca attacttttc cgccagtata
    21241 accgatcaat aaaggaatca gataagttag cattgggctt accatggtag cgaaagcctc
    21301 atttggtaaa taaccatctg ggataaatag agccgttaat acaccccagg caatcagtgc
    21361 tccaatattt ggcatgacca tgcttgataa agtactgcct aatttttgta ccctcgcttt
    21421 tagcgaaatg ttgtttttgg gaatcgtccg ttccattttg tctctccctc tctccttttg
    21481 atactctaat tgtatccgct tactaaaaag agaaatagtc gtactttggc acgaaacttt
    21541 gtgccaaagg tttgagtatg tcaacaaaat aaaaagattt cggtatcaga aaacttcaat
    21601 tttaaataaa actgttattt taattttttt ataatatgca aacaataact tttgttagca
    21661 tttaatttag atgttttcca ttctattatg ttattaaatt ttatattgga ggcctatcta
    21721 tgacattact gatcaaaaac atgaacatga aacgtggtaa aaaacgtgtg attcgacaat
    21781 tgaatctttt agtagaatca ggagaagtag ctgctttgat tgccccgaac ggatttggca
    21841 aaacaacttt gctgaatggc attgcccaac tgctgccaac aacttatgat caattgtccc
    21901 ttcacaacat ctctcctact gcttatacag attatcgccg gtctttcttt tttctagaat
    21961 caacgaatca gttatccgac tatctatcgg ctgttgatta tttgactttg atcaaatact
    22021 attggcagag caaagtagaa tgccaaactg ttttccaacg attgaagatg actgagtacc
    22081 ttactttgcc gattggaaaa atgtctctag gaatgaaaca acatgtcttg cttgctgctt
    22141 acttgatcag tgattcatct gtcatgttat tcgatgaacc gttgaacgga ttagacccag
    22201 gaagtattga tatcttgaat agcctcatca gacaatggca gaaagagggg aagacgattc
    22261 ttctttcttc ccacgctatc gcgaatatcc aatcgatttg ttctaaagca tgttttctaa
    22321 aaaatggaga aatcgtcaca gaatcctcaa atatggaaga gattttagct atttatcgta
    22381 aattatatga ggtgtaacta tgtctttact tacatttgaa cgcaatctct tactaaaaaa
    22441 caaagtaaac tttttatttc ctctattgtt ggtcgtgctc tttgcattcc cacttttttt
    22501 tgatcataaa cttgcttata cagaattcga tgaactgaat cataactatg aagagatgca
    22561 acgtttgatt gaaacgttga aagaagatga gaatgagaaa gaatttgtgg aaagtttaga
    22621 gaaaagcaac aaactgatcg aagcaatctt acatgcaaaa aacacaggga atgtccagca
    22681 aatggtcgaa gcaacttatc attatgaaaa agatatactg gatcgactga tttctggtca
    22741 aagacaagga attccaatca ttgaacaaca aaaacgtgtc gagttgctcc gttatatgaa
    22801 agaacatcag atccaaagat attctatttt tgacttgcca gcgcatctct ctttagcaaa
    22861 ttactacgaa aatatttttt caggaatgat ttctagtttt ttgattttat gtataaccgc
    22921 cttgtttctt tcttccatca tctcttatga aaaaagaaag caggtaatct ctctagtcaa
    22981 tctattaccg gatagtatgg taaagaaaca tagcatccgt tttaccattt attatggtgc
    23041 agctatgcta agtctcgtga tgccattttt gatcgtctct atactagtaa taatcaaaaa
    23101 tggtttggga gacttccgtt atcctgtagg aacgatcatt ggtcaggaga tacgaatcct
    23161 gccgatgtat gagtatcttt tccagagctt tctgttcctg ttgttgtggg tgctcttttt
    23221 aagtacaatc agttttcttc tatctgcttt atttgaacat tctctagtca atcttttagg
    23281 tacacttctt tgtcttttct tagcagagta tcgcttgttt tcttcgattg gatggataga
    23341 atcaatcagt cattacttgc caacttctta tgttgatttt caaaatgtga tcataggagg
    23401 agatattttc tcgccactag cttcagaaca ggttactttc atgaatggta tcttgacact
    23461 tggtatatgg agtatcgttt tattattcat aggtatgggc actatctata taaaaaagag
    23521 ctattaaaac acaaaaataa taggggcggt gacaaaaacc ttgtcaccgt cccctatttc
    23581 aaataaacgg ttttctagaa taaatagttt tcaaaagctt gcatccgcag taataagaac
    23641 ctcccaacaa aaatagctcc tcttattttt atcttggttg aagttattac ttgatgcaag
    23701 gcagcttttt ttactgtgaa cattggttat atatgtccat gttcaaaaat catttggact
    23761 tcttcaatgg tttccgcatc actgagcgcc attacttggt cgatgtctga acagaaaaag
    23821 gctaaatctt ggagcaccgc taactgatgt tcttttttta aagccactac aaataagagt
    23881 gtcgcgattt ttggttcctc ttttgttcca aagtccacac catcaggcac ttggactaaa
    23941 tagattcctt cttctactac ttcttcatct ttgtcagtcc catgtggcaa ggctacgaaa
    24001 ttcccgatat agacactcgc tttttcttgt ctttcgatca ttgattggat atatgccggt
    24061 ttgattcccc cttgttgggt catccactgg ccgatctcgg tcaacgcttc tttccaattt
    24121 tgataggatt gatcgagcaa tattttttct gctggtagct gtatcatctt ttcaccttca
    24181 tttctttatt tcttcaatca ggattttcgc taacaactga taaacgattt ctttatttcc
    24241 tgagcggaag atctcggtgt tcaagtcatt catgatgatt gctccactga tctttcctaa
    24301 aagaatcccg tctttttctt ggattggcga aggcgccagc ataactaaca tacgggacaa
    24361 atcgactggc tgtctatcca tcccttcaat ggtcaacgga tgtttcaaat tgaaaataca
    24421 aaagatcggt ttagaaatca atgagctgga tgtatggaat aaccccatat ttgtatgagg
    24481 aataccgata ggtgcttgtt catatcgctt aagcagccgg cgatgaacca cctcttcatc
    24541 cgttactagt ccttcaggaa aataaggaaa taagctgcga atcgtttcgg aaatcgtctc
    24601 aggattatcc agttcttgga taaaaaaacg accgagtaat tgattgattt gatccatgaa
    24661 atgcattgtt tcctcataag tatcttctat catccactcg ttattgctgt tatacgtaat
    24721 tttttgcctt tttttcttct caatatgcga aaaagcttct tttagctgct tgacttcatc
    24781 ttctagcagt agcggggaga ctaaaatata ttttccaccg tatcctggta aaatagatgt
    24841 agaaataaca acatcatatt cttcttgtaa atcgattttt tttaaatcag caatccgata
    24901 gaaatgaatt tgttggatga agggaaaata ccttttcaat cgtagttcca acatgctagt
    24961 agaagctaat ccacttggcg aaaatcctgc aatactgata ttgctttctt tcggacttcg
    25021 ttccaatgaa ttcgcaaaat gaaggaccat ataagcgatc tcttcttcgg ataatttttt
    25081 ttcttgaaaa atccgctcta ctgctttacg gattgcattc gcaacttcct cgtattgaac
    25141 catgattttt tctaaaatcg gattggtcag tgtctcttct tgtaaaacgg ctcttgaaaa
    25201 cacaccagat aaatgagtca acagcatttt gtacaaagtt tcatcttcat aaaaatggac
    25261 attcgtctct tcacttactt cttgaatcag ttgttttact aaataagcaa gctgtgtatc
    25321 gaacgtctct tcaaagaaat tctgatcaaa tgagttcgtg aagtcattta ataatcctgc
    25381 aaaaaagacg atctcattta ctggataaag ttgttttgtc tcagaagcga tccgtgaaaa
    25441 caatcgttta gctgtctgta gtaattcttt gtttgccaga ctattatatg tctcatttgt
    25501 cagtaaatga tttttcgtca cacgctccat cgtcaatgtc agcatcaaaa ccaaatgttc
    25561 aagctttcta tcacttagat tggtcttttt ttggatcaat tcttccccga ttactttgcg
    25621 tgcaagagat agatgttgcg gatcaacgaa tcgaaaaaaa taattcagtg atgcatcgaa
    25681 agaaatacta tgaaaaagtt catattcatt gatttctaat tctaatacat tcgctgtcag
    25741 aagccggcgg tatttttccg gtccttcgat ctcgtatccc tggttccgaa tgatttccaa
    25801 aggcagttgg cgaatacttt cttccaactg tttgatatct gcataaaaag tggtattact
    25861 gatgcgatat gattccatga aatgagcaag actcaacggc tctgtcgtca gtaacaactc
    25921 ggctaaaatc gcgtgttgtc gtttagcagt ggataagtca ttacttttcc catctgtatt
    25981 ttcttttaat tgctctaata cttcttctga tgcctcaatg acaaatgagc ctctagctgc
    26041 ttttttcaaa gaagcattta ccgatttcaa tgagacttca agattatcta attcgcggta
    26101 aatagtccgt ttactgacct tcaataaatt catcattttt tggatagata caggtgacgg
    26161 gctactgaga aattctgcta ataattgttt ttctctgggc gataaataca tcttatattt
    26221 ctcccttcac gagtttttcg tattcattcg tagctaccca atgttcaact agaactagcc
    26281 gagggatctt ttcttctagc tgttcagtta cagctagttc tctttgtgcg atataaatcg
    26341 tttcttcttg gattttgatt ttttctattg attcttttga gataacaaac tgttttttcg
    26401 ctttttttgc taattgactt aaaatcgtca tgcccatagt ttgtgagccg cgtctgttgt
    26461 ttttatacaa aaatacaata ttagcaggcg acgttttttg ttctgtgata tctgctatct
    26521 cttttttttc ttcccgcttt ggaaattgga tcgcttcttt tattcgtgac agcaagatat
    26581 ctgtttccaa aaagtgatcc agtacataga tttgactttg aggtactttt tctcttgcaa
    26641 gacccgctag ttcatgttgc acgataacca gctgatttac ttggttattc aattcataga
    26701 tagagctgta tccgacagaa agatcgattt tttctgctgc taatttcttt ttcagcaggg
    26761 cggctcccat agcacttgat cccatacctg agtcgcaagc aaaaatgacg gaatgaatga
    26821 tagatggttc caagatttct cccctttctt tgaccgtttt gttctctttt tctttatttg
    26881 gtaatttcaa tagtatcgct gcacaaatga aggtcacaaa tgtactgatt gctatcccta
    26941 tacttacccc agctagttgt ggataaggcg cattggcaaa aatcacgagt agtgacccag
    27001 gcgatacagg tgcagctaat cccgtatcta accattgaaa tatcaacgtg ccgcttgccc
    27061 caccagccat tacagctaaa aatagatacg gtcgcagcaa gacaaatgga aaatagattt
    27121 cgtgaatccc gccaatcgca tggatcatca atgctccact cgcatttact ttttcctgtt
    27181 tttctgcaaa acaaaggata gaaagtaaga tccctaaacc aggtccagga tttgcctcca
    27241 tcaaaaataa ggaagagtac ccgactgttt gtgagaattc gattcctaaa ggtgtcaata
    27301 caccatgatt gattgcatta ttgaaaaata gtacttttag aggttctaag atcagactga
    27361 caagtggtaa cagattcatt gtgactagcc aagacacaac ggaggcgagc aacaagtgaa
    27421 cactatttaa aactggttca ataaaaacga ttccaattat acaaataacg ctaccagtca
    27481 gtcctactag taggttgctg acaagcattt catatccagc cttgaatttc acatggaatc
    27541 tagaaacaaa ttgttcatat acgatgccgg caagcggccc gataatcatc gctcctacga
    27601 tttgtggagc attggaacga gcgatcaacc ctaaagaagc aattgcaccg gcgactgccc
    27661 ctttttgtcc ccctaccgat ttcccgcctg aataactgat caagatgggt aaaagcagct
    27721 ggatcatcca atggcatacc tcttctaatg tggtgtgcaa atcgccagac acaccttttg
    27781 ttgctaatga caagattccc caagcgataa acagacttag atttggcatg atcatttcat
    27841 ttaagtgatg tccagctttt ctcagttgtt tcatagacac gcgactgctg ttgtttgctt
    27901 tccatttgta ttccatcttt ttcaccagac ttctatttat tgatggacgg attttatgta
    27961 gaaaaagcag caaacgaaaa accgtctgct gctttcagct atctgggaac tatttgcctg
    28021 gttcagacgc aatgattgct agttcacctt ggaccgccca ctcagaaacg ccatttggaa
    28081 taatacagct tgttcctttt tctaatggat atgtttttcc gtcaacgaca aggtctcctg
    28141 ctccttcgat taccgtcatc aatgtataag gggcttgttt tttgaaagat gtgattcctt
    28201 taatatccca ctcatataca ttgaaaaact cagtttctaa ataagtgacg atcgatgaat
    28261 tcccttttcg gacttctttg atttgtaatt ctggtgtttt cgcagggaca gttgttacat
    28321 cgatcgattg ttggatatgc agctctcttg tttgtccttg cgcatctttt ctgtcgtagt
    28381 catacacgcg gtaagtcgtg tcagaacttt gctgtgtttc taaaatcatg attcctttac
    28441 caatagcatg gatcgttccg cttggtacat aaaagaaatc acctttttta actggtactt
    28501 tcttcaaaag gtcatcccag cgcccatctt tgatcatctc agctagttct tctcttgtct
    28561 ttgcatgatg accatagatg atttctgcac caggttcagc atcgatgata taccagcatt
    28621 cagtttttcc taattcacct tcatgtttca tcccataggc atcatctgga tgtacttgta
    28681 cagatagatc atcttctgcg tctaagatct tgatcaataa tggaaacact ggttctgaag
    28741 gatggccaaa caattccttg tgttctgacc atagttcatc taatcttttc cctttgaatt
    28801 cgccattttc tactacactg acaccatgag gatgcgcact gatcgcccaa tcttctccga
    28861 ttttatcgtt agggatatca aaaccaaaga ctgaacgtaa acgacttcct ccccaaattt
    28921 tttcttgaaa aactggtttt aaaaacatag gttcctgcat aattcaaact cctctcattt
    28981 ttcatagcta ttatagcatt atttcggtaa taccgctttc tctttcaaaa agttttcaac
    29041 aatttcatct cgacatatga gatactccgc acgatgatcg acaggatgta cgcgatttga
    29101 caaaaatatc aacgcttctt gtgtttttat atcaatcaag aaaaacgtac ctgtataacc
    29161 agtatgaaaa agaatcgcat gaccgtcttc tacatccttt tttaagtccc atcctaaaga
    29221 acgatagcca ttttttttcg gtgtttgatc ttgcagtaaa gaatcaatcg tctcttcttc
    29281 taaaaaacga ccattacgga tttttccctt gttcaaatac atttggacaa aagtaatgct
    29341 gtctttcaaa tcaataaaga gacccgcgtt acctgcatgt tcagccaaaa ctcttgcttt
    29401 cggatcatga gtttgtccac gcaaaacctc acccgaaggc agttgttctg tgggaacgat
    29461 ttttttggaa ggaaaaggaa gaaataagct gttcatcatt cctaatggtt ccaatacttg
    29521 ttcttgaaag atttccactg cagtttcttg gtaaatttcc tctagcatca acccaagaag
    29581 aatcgtaccg gcatctgtat attttacttc ttttcctagt tgctcacctg gttctagctt
    29641 caaataagcg gcgatcaatt cttcctgaga tagcttatct ctgtttggta tccatgtttt
    29701 gatatcagaa gtatgtgtca gtaaatgacg caaagtgatt gtctgatttt caaattcagg
    29761 aagatattta tgaagcggtt gatcgagttc tatctttcca tcttctaata gacgtaaaac
    29821 taatgaagtg gttgcaacga ctttcgtcag tgaggctgca tcaaacaaca gatcttctgt
    29881 catcagctgc ttttcaggaa cgacttgggc aaagccggct gtaaatgttt ctacttgatt
    29941 ttcttttatg aaggcgactg ctacacctgg ataaaatcct tcttccatct ttttcaaaat
    30001 aagctgtttc gtgtgtggat acatcttttt ctcctgcttt tctatcagat tcttacagtc
    30061 caagcttaaa gcttaatcta tgaggaatca acctttttta cacaaaaaaa ctgcgcgaaa
    30121 aaccgcgcag tttcccccgg tatactattt tctctttcta tctctagaat aagcgatagt
    30181 atgaccgttc ataaaatgac gagacatgcg acaatcgtta agaaagaaaa agttaagcgt
    30241 cgcatcgaat gtcagtttta ttcttttctt ttggctcact gacgaaccac cattcgattc
    30301 ctatagcatc atagattgtg agtactgtgt gctttttgtc taagaagaaa tcaactgata
    30361 gttgctgcaa gtgagcaatc agtccttcca tgctttcagt atcgatcatg aatttcagaa
    30421 aatcaagtcc aatcacttca tctgtaggca aatcaattgc gcctccgcta gcttctgtca
    30481 aaccaacatg gaaatctcct tcattcaata catggatgcc ctcttcttca ttatgtacct
    30541 tcaaaccaag tacatgatta agaaattggc gttcttttgt taagttagta acatttaaat
    30601 ggattttttc aaaataagaa cctgttgata gtttactgaa tttttcttca gattttctta
    30661 ataatgcttc ttgatccatt gtttctggaa tttctctttg gtcgttctta ttctcttcat
    30721 ggtaaatttc caattcattc tcttctgggt ctacgagcaa aatccccata cgtccatgat
    30781 cttctaaagc ttctttgatt ggataattca ttttacggat acgacaaagg atatctgcca
    30841 tttcttctgt gctagggata atcaatgaaa aacggttcag ctttttgatc acccccatgt
    30901 gatcattcgc tctaggactt tcttccagca gcaacatttc acttccagct tcttctgttc
    30961 ctaaaatggc taattcgttt tcttctcttt tcaaatcaaa gccaacaacg ttcttgtaaa
    31021 aatcaatcat tttatctcgg tctttcaccc gaatggctac actttttaaa taggtggcag
    31081 cccccaattg aaaatctttc atatttcttg ctcccctcta cgtcaagctt tattttaacg
    31141 atggattctt cttctttcaa gcaataactt tgattttgtt aaaaaaaatg tgagaaaatt
    31201 cgcattttgc ttgttttttg taaattaatg agtaaaaact cttataaaaa gaggttgtga
    31261 aaaagctgtt ctgcatcaag taataagaac agccaaacaa aaatagctcc tcctattttt
    31321 cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttaa ggaagaggtt
    31381 gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttctacat
    31441 ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt taccaagagg
    31501 ttgtgagcct gccgtttaac tccgagtcac aaggagcaat ttcaaaaaac agcttctcag
    31561 gtttgttgac tattgtgact tgtgaccgag gagttggcag gagaacaccg tttaaggaag
    31621 aggttgtaaa atcagcggtt tgacctgagt attagagata caaaacggaa aaatagcttc
    31681 tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca ccgtttattc
    31741 ggaaaaaggg actgtgacac gacttttgtc acagccccta aaaaaaataa taaatttgta
    31801 ctattctatg aagcttcttc ttcagaaaac tgactgttat aaagatctgc atagaatccg
    31861 tctttttcca tcaatgtatc gtggtttcct gtttctacga tcgaaccatg attcatcaca
    31921 ataatgttgt ctgcatcgcg tattgtagac aagcggtgag caacaacgaa acttgtccgg
    31981 ttttccaata ggcggttcat cgctttttga atcagtattt ctgttcgtgt atctacacta
    32041 gatgttgctt catcaaggat caggacatct ggatttgcta agaatgcccg cgcaattgtg
    32101 attagctgac gctgcccttg cgaaatattg cttgcatcct cgtttaatac agtgttatat
    32161 ccttctggca atttacgaac aaaatcgtct acgtgagcag ctttagcagc ctcaatgatt
    32221 tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt catagatact tcctgtaaac
    32281 agccaagtat cttgcagtac cattgaaaac tgtgcgcgca attcatcacg tgaaagatca
    32341 cgggtatcta caccatcgta acgaatacta cctccagaaa catcgtagaa ccgttcaaga
    32401 aggttgatca gtgtcgtctt ccctgcacca gtcggaccta caatagcgac catctcacca
    32461 ggtttgacat ccaagttaaa atcagtcatt agcaagttat ctggtgtgta gccgaattga
    32521 acattttcaa aacgaacttt gtatggtgta tctttttctt ctggaaggcc agatttttca
    32581 ttggacatct cttcttcatc caacacttca aatacccgtt cggctgaagc aactgttgat
    32641 tggatagtgt tcatcaaact tgcaagttga gtgattggtt gagagaactg tgttgtatat
    32701 tgcaagaacg cttgaacatc ccctaatgtc atgttcccat ttgcaacttt tactccgcct
    32761 agtacagcga cgaacacata gcctagattt ttaacgaaat tcatcaacgg catgatgatt
    32821 gccgaaataa attgagcttt ccaaccagcg gcatacagat tctgattttc tttttcaaat
    32881 acttcctggt ctttttcagt atgattgaat gttttaacaa tggtatgacc accatatgtt
    32941 tcttctactt ggttattcaa taaacctaaa cttttttgct gatctgcgaa aaacacttgt
    33001 gatttcggcg ctacgatcat cacgatgatc aagcttaaag ggatcatcaa taacgcgact
    33061 aacgtcaatt gccagctgat tgaaagcatc atcacgatga ccccaatcaa tgtgactgca
    33121 ctagttacaa attgagtcaa attttgctgc agcgtgcttg cgatgttatc catatcattg
    33181 attgctcgag acatgatatc cccatttgta tgtgtatcat aataggaaat cggtacacga
    33241 ttcattttgg cttccaaatc atgacgtaat tcataaactg ttcgttggga aacacgtgtc
    33301 ataataaatt gctgtaaaaa gttgaataca gcagaaatca aatacatcac gattactgtc
    33361 gcaatgatct gcgcgatttt atcaaagtct atcggaaatt ttgttacttg ttggccttgt
    33421 tgcatctgtt gagcgccagc cattacacct ttgaatattt ctgtggttgc tttccccaag
    33481 acctttggtg tttggatttg gaagatcacg gctgctattg ctagtacaaa tacagcgatg
    33541 atcgcaacca tacgtttaga catatagcca aacaatcggc gtaccgttcc ccagaaattt
    33601 ttaggctttt caccttttac accgagattt ctgccaggtc caccatgtcc tggaccgcct
    33661 gctggtccat gagagggacg tgtgtttgtt ttactcatgc taagtcctcc tctcttagtt
    33721 gtgaatgaac gatttcttga taagtttcgt ttgttgctaa tagttcttca tgtgttcctt
    33781 tgccaaccag ttttccttca tctagcacaa ggatcatatc tgcatccatt actgtactta
    33841 ctcgttgagc aactaaaacg gtgatacttt ctttcatatt cgttttcaat gcttggcgaa
    33901 gattggcatc cgttttgaaa tccaacgctg agaaggaatc atcaaacaca tagatatctg
    33961 ctgttttgac taatgcccgt gcaattgcca gacgctgtct ttgtccacca gagaagtttc
    34021 ctcctccttg ttccacatgg ctatctaagc catcttccat ctctgaaaca aaatctttag
    34081 cttgtgcgat ttctaatgct ttccaaatca tttcatctgt tgcatccggc ttaccatact
    34141 gcatattgtc tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca aagcccatta
    34201 attctcgcaa attatattga gtcatgtcac taatgtccgt tccatttatt tggatcgaac
    34261 cagattcgat gtcatataat cgagggatca aattgactaa ggtcgtttta cctgaaccag
    34321 tcccccctat gattgcgacg atctcgcccg acttggcttg gaaatcaata tcttccaatg
    34381 caagtttttc tgctccatga tatcgataat tgacatgatt gaaggccaat gttgcgtttt
    34441 ttccagcaaa agaaactgtt ttaggatttt cagggtcatg gataccgatt ttttcatcca
    34501 acacttcatt aatacggtca gctgaagctt gtgcacgagg caccatgata aaaatagcgg
    34561 aaagcatcat aaagctgatc aagatctgca tcgcataagt cataaatgca atcagattac
    34621 cgacttccaa agtcatatct gctatgtagt gtccaccaaa ccaagtgatt gctacgtttg
    34681 taccgctcat gatcaatgtc atcattggca tcatcaatgc gacgatcgta ttaactttga
    34741 ttgccgtatt tgtataatct ttattcgcta aatcaaaacg attttcttca aagcgtgtct
    34801 tatcgaatgc tcgaatcact ctgacccctg tcaatccttc tcgaaatacc aggtttaaac
    34861 ggtcagtttt cttttgcata cttttgaata atggaacagc aaagtacatg attccgccga
    34921 tcaaaataat cattactggg atgacaacta agaagatttt tgtcaactga tgatcttttt
    34981 gataagcaag tatgctcgcg ccaatcaacg tgattggtgc attgatcatc atacgtaaaa
    35041 acatttgtgt aaccatttgg atttgagtca catcattggt cgttcttgtg atcaaagagg
    35101 ccgttccata tttatcaaac gcatcttttg tcagactctc tgatttttta tagacatccg
    35161 tacgcaattg cttacccagt ttctgtgatt cacgagtagc aaaaaatgta tttgcgatag
    35221 cagctaagat actgatcaaa gaaaagccga tcatcacaaa acctgtatgc caaatataat
    35281 cgacatctcc ttgcgctacc cctttatcaa taatattgga ggtaagtgtc ggcaaatata
    35341 aatctgccag tacttggatc accatgaaaa ctgcagctgc aattgctgaa gtcaaggaga
    35401 tccgttttac gatttttagc atctagttcc cttctttcta tttatatact ccgtttttca
    35461 gccaagaaag tttcaaattg aattcactga ttgcttgctc tactgagtaa tcttcagatg
    35521 ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc actaattgga
    35581 tcagtaattc caattcatga tcatttttta ctttcagctt cgtccgatcg atcaattcaa
    35641 gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt ttttggcgac
    35701 tcgtatgagg agcgactttt cttgaggcac ggtaatccat gttcataaac aagttacgat
    35761 aaaaagaagc attctcgcct tccaaaatct cataaatcat tttagaaaaa taggattcga
    35821 atcctttgaa tagatcacca tcggattctt tcattagttg taacatatct ttagagctgt
    35881 cacgtctcat tgtctcaaag taataaaaat aaagatcctc tttatcttca aaatactgat
    35941 agaaactgcc tctagggata tccgctaatt tgacgatatt agcaatcgaa gcttctttta
    36001 agggtactct agaaaactcg attctcgcag cctcgattag tcttttttgt ttttctttag
    36061 gcaaatggaa aaatgtctgt gtcggcatat ggattctcct ttctttcaag atgacacatt
    36121 gtcatatgac gctatgtcat tataacacct cctacctgtt ttgcaagaca tttgcatagt
    36181 ttttttcatt atcatccgat acattaaaaa agtgataaga ggaatccttc catttgaatt
    36241 aatgagagtt gcccattaaa atagccacat actaaacaaa ggatgagata aatggcccaa
    36301 tcaattagtg tccaagactt caataaattg cctctcgatg ctgacagcat cgttttagat
    36361 acaagaaata ccttagatta cacaaaaaac cacttaagcc actcgctttc tatccccgct
    36421 gctatcttgc caagacaatt aaaaaatcta gatccaaaga aactttatta tattctaagc
    36481 tatacaggca ggcgatcaga agcattagcc gatcaactta ctgctaatgg ttttcgtgct
    36541 gtttatatca ttggtgggat ggaagctctt cagtcgagtg ttgcttgaat attgcttgtt
    36601 agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc tcttttatac cctgtctttt
    36661 tttagttatt tattcttcaa ccttatcaat atgacttgat agccttttct ttttcgagta
    36721 aactgatttt gttagtcata gttataacaa tcatagcaat aaaaggagaa ggtaagcata
    36781 tggcatctgt tttagtagtc aaaggtcatc ccctaactgc agaagaatcg cgcacagtta
    36841 aggcattgac gtctttttta acaagttata aagaaaatca tccagatgat gaagtgactg
    36901 ttttggaact ttatcgcgat gatattccgg aaatcgatga agaattatta tcaggttggg
    36961 aagcattacg tgctggtgca gaattcactt ccttatcaga aagtcaacaa caaaaaattg
    37021 ctcgtttcaa tgaattgact gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg
    37081 ccttatggaa tctaaatatc ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag
    37141 ctggaaaaac gttccgctat acagaaaacg ggccagaagg attagtgaca ggtaaaaaag
    37201 cactgcacat ccaatctaat ggcggtgtat acaacggaca agactttgcc tctcaatatg
    37261 taaaaggtat tttaaacttt gtaggcatcg atcaagtaga tcagctattc atcgaaggaa
    37321 tcgattatga ccctgaccgt gcagatgagt tgatgcaaaa tgcattagat aaagctgctg
    37381 ctttaggtaa atcattttaa aaattgagat aaatgcatac aaaagagaca gaggaaaaaa
    37441 tcttcaaagc agcatttgtg ccttggatat tttcccatct gtctcttttt gttattcttt
    37501 ctgctttttc aacggatttt cttcataaag agaagagatt gttgtgtacc attcaaaaac
    37561 atgagtaatc actactttag ctgctgcata aacaggaatc cccaagatca caccgatgat
    37621 tccgaacaat ttcccagaag tcagcagaac taataaaatc gttactggat ggattgctaa
    37681 ctgattccct agtactaatg gagagatcaa gcgtccttct attgtctgct ctactgcaaa
    37741 aacgatcagt actttgatga tcatgaatgg acctgctaca atggctaaaa agatagccgg
    37801 aatcatcgct aaaaatgatc ctaagtaagg gatcaggttt aaaaaacccg caataatccc
    37861 caacgtgaca gcatactcta aaccaatgac cgcaaaacca atcataaaca tgattgccac
    37921 agcaaaagcc acagttagtt gtcctcttat ataagaagaa acttgactat tcatttcact
    37981 cagcacattc aacgtcggtt tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc
    38041 taattgttta ccgtctttca atagataaaa taagataaac ggcatcgtca gcagagccac
    38101 taaaatagtc gctaccgcgc cgacaaaact tccaagactc tgaactgtac tcttagatat
    38161 atcttggatc atatcgcctg ccgaattcat gaacttatca cctgctgttt ccaactgttc
    38221 gcgaaattgg ttgaacagcg ggtcccgcaa aatctcggtt aatttattat caatcgtatc
    38281 tacatattga gggaagttac tgatgaagct cactgtttgt tcctgaatct tagggatgat
    38341 cactacgctc ccccatgcga ttagtgcaac aaccaaaaca aacagcccaa tgatactgta
    38401 aagccttggt acttttcgct tttcaaagta atcaactacc ggattcatca agtaatacaa
    38461 gattcctgct aaaatgatag gcagacctac gattgctaaa aattgccaga ttggtgcaaa
    38521 caaatatgaa acttttgtaa ataaaaagac gatcagcaat attaacaata caacaagtaa
    38581 ggctgtgact acttgattat ttaaaaacca gcgccaaaac cacgataccg gtctgttctt
    38641 cttatttttt ccttccataa ttcctcctgt tagttataaa aaatttggct tcccaaacca
    38701 atagcaggca gtctatgcgg atgaagatag attccatttg caatttgtcc ttcttctgga
    38761 caagcatcaa aaatcaatga tacgtgagct actgtcttta aattcccatt ggccatcgga
    38821 ccgacataat cgatcgtata tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa
    38881 gaaaaaggtt cgtcttttga tacttcttga atcaccgaat attttcgtaa ggcagctgtt
    38941 gctgttttat cgaacaaaat cagcatgggt tcttccttgt ttattgcttc tgtcccaatt
    39001 tctgtaatct ttgcctgaat cattgcactc tctcctttaa ttgctttcaa aaacagtata
    39061 acacattctc attctttagc cgtgtggaat aattatgtga tagaataaaa acgaaaacta
    39121 tttcattaca attaggagga atcagctcat atgatcgaaa agataatttt aggaacctat
    39181 acgcgtcgcg aaagtaaagg aatctatacg atcgaccttg atacagaaaa agaagaatta
    39241 gccaaccttt cattagctgt tgaagaaaat agccctacct atgtagcaaa aagtaaagct
    39301 ggcaatcttt acacagttac tagtgtggat ggattaggtg gtgccggtgc gtacgataag
    39361 gattttcatt ttttgaatgc ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt
    39421 gatgaagcac gtcaattact atacggggcg aactatcata aaggtgaagt aaatgtctac
    39481 catatattag gagatggcga actgaaagca gcagattcaa tattccatca agaagcgaca
    39541 ggtcctcata aaaaccaaga tcatgcgcat gtccactata cggatctgac tcctgaccaa
    39601 cgattagtcg tatgtgatct tggtacagat cgtgtgtata cttatgatgt gtccgaaaac
    39661 ggcaaattga atcttgtaac taccttcact gcagagccag gaacaggccc tcgccatctt
    39721 gtctttcatc caaatggaca attcgcttat ttatttggtg aattagacag cacagtccgc
    39781 gtgctttctt ataataaaga aacaggtgct tttgaagaat tacagaaaat atcgaccttg
    39841 ccagaagaat tcgacggtga aaatggaggg gcagctatcc gcatttcaaa tgatggcaag
    39901 ttcctttatg cttctaaccg aggacataac tcgattgctg tattcgcagt ttttgaagat
    39961 ggttcccaca ttcaaaacaa gcaaatcatc tcaacagaag gggattttcc ccgcgatttc
    40021 gcactagatc caagcaataa ttttgtcgtg tgtgccaatc agaacaccga taatctgaca
    40081 ttatatcgcc gtcatcctga aacaggatta ctggaattac tgaaaaaaga tgtctatgta
    40141 ccagaatgtg tctgtgttta ttttgatcgt tagtaagaaa aggagaatga aatgaatcag
    40201 caagacgtat tagacagact acgggaagaa ctaaatattc ctttctttga tgggaaaatc
    40261 gaagataaag agtatagcga agaggaatat cagaaactca aagccgattt acagaactac
    40321 tttgaacaat atgtacagaa tgtagaaaac taatcagctt tccatactat ctgaaaagaa
    40381 catcaggaca agacttttcc gtctatcctg atgttctttt ctacaaataa aaaaccgccg
    40441 agatggttag actcgacgga aaaggagtat ttactcgatt ttgagtaagt acaaggttgt
    40501 tgttggttat aggtatgtca ttatcataaa ggattttgat cactctatca taaaggtaat
    40561 attacattta tattagataa ctctagtttt tattgttatc aaagatttct tttatttttt
    40621 caacaacaaa ttagtcattt tttggatggc agctgtcaat tggtctgtcg cttggtcttg
    40681 ttttgcttca gatgtattga atagatcttc atctacccct gattcaagat cgatactttg
    40741 gatcatttct tcacagcctg ctacatatga aagataagct ttttcaaaat ttttatgaat
    40801 gcccattgct tttgctggtg gacgcaaagt actgactttc ataagcattg cccgatattt
    40861 ttttgttcct tcttcaaata acaggatgat ctcttcaaga gtcgatttgt ccagttcact
    40921 tgtttttcct tcatcaattg cgtttctgat cacttcatat ttcggatgca ttccttctcc
    40981 gattgcttct gtttccttga cgatttgatc gatggactgc acataatagc caatattcgg
    41041 taacatgttc gttttcctac tttctccatt gttgttatta tttattctac ttgtttttat
    41101 cctcaaatga caagtacttg aaaaaattta atggtttcta tacagctcgc cattcttatt
    41161 cgtgctcaaa gaaaccatct gctaaactat attgtttccc tgcgacaatg tcatattgga
    41221 tcaattgata ttcgtcgtaa atctcttgtt gcgagcgatt gaattgcgct tctttatacc
    41281 actgtccgtt ttgatagtac agttctcgtt caaacgccgg aagttcctgt tctaatgata
    41341 aaagcagctg ataaaagcca tttgttgtct gattcgtctg ttccattaga ttcgctgcaa
    41401 aataaaacgg actggtgatt gcatcgtgag tcgtttgctt ttctaattct cctttactgt
    41461 cgaacataag aaattgtgtt tcatgcaatg tttgtttgtc attttttgct tggatcgtat
    41521 ctgaatagat ccctggaaga tgatcccccc aaaaaacaac taaagtcctt ctagaaagat
    41581 tcttcaattc ttccgtgaac tgcttcagtg ccgtgctgct ataggaaata tcttgcagat
    41641 aattctctaa gtcaagtgtc ccacttccat ctgaagtttt tgcaggatag tctagattat
    41701 catactttcc atcgtagggc atatgtgtct gcatcgtgac taaatgaatg aactgaggtg
    41761 ttttttcttc cttgagaagt gtcattactt ctttgtaagc tgactcatca gaaatatatg
    41821 gattattctc tatcgtatcc gtataggtca tcgtacgctc gctgatgaac tggtcaaatc
    41881 ctaatgtctg ataaacatcc tctcttttat acatcgaagt attgtatgga tggatcgctg
    41941 tcgtctggta tgattgtgcg tttaacgctg aaacgataga aggaagctga tccatttttg
    42001 gtacaagcat cgtgtaaggg gtggtgagtt gcgcattaaa taacgccata gaaaaaccag
    42061 tcaatgcttc aaattcgata tttgctgttc caccgccata gttttgagaa agcatattgc
    42121 cactatatgt ttgatcagct acttcataat aatctgccaa tggctcacca gatacttcta
    42181 cgccgtttaa tcgactagga tctgagaaac tttcactcat cacgaacacg atgttcggcg
    42241 actcttcctc tacagtttta tttttttcat cggctagctt ctggtatttt tcagtgattt
    42301 ctttgatctt ttctttcgaa tatccttcag gttcatccat tggttctacc ttcagattgt
    42361 acaaaaaccc accgataaaa ccagtgttat agtagttcat tttctggctg taaggtatcc
    42421 atagtgccgt tttattatat gcttttcgca acaaattatc gggattgttg aaattactga
    42481 tataccctag cagtccaatt gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc
    42541 ttcctttttt caggcttcga aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg
    42601 cagctaagat cactgtaaac agcatagtgc cggtcatatc tttcagcaat ccgatttcag
    42661 tgatcatctt caaatcgtct ggataaatcg gttcttgccg ataactcatt ttcatataat
    42721 ttgcaaaacc taatacaccg atggctacga catacgtaag ggaacctaaa gggacagatc
    42781 ctgctagtgc aatcagaaaa ataagaataa tcagcaagac gagacaggct aagaagaatt
    42841 tttccgtatg ccaagaaaaa gcaaatttca acgcaaggtc tacggataga tcattttgac
    42901 accactgcaa aaataaatta ctaaaaacaa caagaaaaaa gatactgatg attgctgctg
    42961 ttccttttaa aatctttttc tgtttggtcg aaagtctgct tatatatctt ttcaaatgct
    43021 tgctccttca tttctatcaa taaaagaatg ttgcttctct tattttagtt gtacgaaatt
    43081 cgaaagtaaa gaacggaaag aagaattaat gaaatccaaa tattctcttt ctttatggcc
    43141 tttctggttt ctttaataag cggacctttc gtactccaga aattttttct agctgttgtt
    43201 tcacttgatt gagctgttca aaatcagtac tgtctatgtc aatcaatatg tagacatgct
    43261 cattcttatg gttacgcgat atatctgcga tcgctagctc gttctcattc agcagagaaa
    43321 atatctctgc taatatattg gttttctttt gataaaatat agtgaatcga aacggcgctt
    43381 ggaaaaacat tcgtgcggat ggataattga ccgcttctct aaccgtaccg aacaaaagat
    43441 aatcttttaa ggcttcaact gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc
    43501 cccctagatg aggcaacata agtatacggt cattttctaa gaactcttct tctggaaaat
    43561 cagtcaagta tttagccaga tgttgttttt ccaatgcttg cataacagca tatttatcaa
    43621 caatttcact tcttcctaca ttgataagta cagcatcttt tttcattttt tctatattcg
    43681 cctgatcgat caatcctttt gtatcttctg tcaacggcag catgactacg atgtaatccg
    43741 aggtagaaag aactgtttct aaatctgctt gccgaacata atccaactgg gcatcgcgga
    43801 tagaataacc caagacatcc atccctaagc tatagcaact tagcgctact tttgtgccta
    43861 tcgctccaag accaagaaga ccgatggttt tgccttgcaa ttctcgtccc acataagcac
    43921 ttcttttgtt ttctgcctgt tctaaaatat taggacctgt tagtgtttgt accatacgac
    43981 ttgcttcgat tatcggacgg gaagataaga gcaaacagca caaaacaagc tcttttactg
    44041 cattagcatt tacacctggt gtattcatga cgatcgtccc gttttcagaa gctttttcaa
    44101 cgttgatcgt gttcacccca acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc
    44161 attcatcttt cactttcgta ctacgaataa ctattccttc ggctggcaaa tcatctatta
    44221 ttttgaaata ttccccttgt aacttttcta tctcttgttc tggaatagca tcaatcaaat
    44281 gaacgtgata cataaaaccc cctccttact aacaagtatc tcgaaatttc tgcaattaga
    44341 caactgttta ccatttgact tcctagaagc aaaaagagca gttagagcag agtaggctga
    44401 gtgataaaaa taaggctatg acaagttact gccataagcc ttattttcga gagcaaaata
    44461 ttgagggtga gatatcttga taaatggtgt tccgttagct gacattgata ataagactaa
    44521 atttcaaaaa agttgaaaag caaatttcgt caatctcttc ttatttctta aaactaagtg
    44581 gctttttcac aacctctttt tatctaataa ccactgtttc caactcaact aattttgccc
    44641 acgtttcaat ttgatctgtt gttagattta gcgagacaac ggtatgatga ccgccacctg
    44701 cctgaatcca tgaatggaca ccttcatgga aattcggttt agttttccat aacacacgtg
    44761 caacaggtaa attaggtgct tcagtcgttg gttcaaaggc ttctacttca ttgatcagca
    44821 gtttataatg cgtaccgaaa tctgccatcg atacgactac cccttctcct gctttcccat
    44881 caaatacaag acgcgccgga tcttctctat ctcccattga taaaggagaa acaacgattc
    44941 ttggtttcgt cgcagcaagc gtcggatcta cttccatcat atgggattcc aagattgctt
    45001 cttggccact agctagctcg taagtataat cttccatgaa tcctgtatct ttccctcgag
    45061 ccatgatttt cagcaaccga tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac
    45121 cgtatccttc tgccatcaaa cgttgtacag ctagtccagg tagttgtttc attccataca
    45181 ggtcttcaaa attagtcgta aaagcgttat agcctccttc ttctaagaaa cggcgaatac
    45241 cgatttcctg ctgagcttgt acttttacat gttcctccca tgtcttttct tcataatcac
    45301 catagtcgaa ggtatacaat tccttgtact cttcaaataa ttgatcgact tcttcgtctg
    45361 atacgcgatc gattacttgt acaagatctc cgataccaaa ataatcaaca gtccaaccaa
    45421 attgaatctg tgcttccact ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac
    45481 caaaacgagc cacttttatt ccaaaacttt cattataggc tactgcaaca tccatccaat
    45541 cagcaatctc tttttggatc tcaggtcgtt tccagtagcc aacgacgatt ttattttgtt
    45601 tatttaatcg agcattgatg aacccatatt cgcggtcacc atgagcggat tgatttaaat
    45661 tcatgaaatc catatcgatc gttttccaag gaatgctttc gttatattgc gtagctaaat
    45721 gaagcaatgg tttttgtaga agctttgtcc cgcggatcca catttttgct ggtgagaaag
    45781 tatgcatcca agtgatcaca cctgctactt cttctcgata attgacctct ttcatgattt
    45841 ttgtgatcgt atcagcagta actgctaatt cttgaaggcg gattggataa ggcaattggc
    45901 cattttcatt cagttcatcg accatttctt gtgcatgttt ctttacttct cgcagtgctt
    45961 cttccccata taaatgctgt gatcctacaa caaaccaaaa ttctttttct ccaatattca
    46021 acataattag ccactccttt tatttgtttt gtccgtaata cgcatttttt ccgtgttttc
    46081 gtaaataatg tttatctaaa atccgttgag gcagctcttc tgaaaatgaa ttgagctgtc
    46141 tggtaaataa attcatcttg catacttcat ctagtacgac tgcgttcatt accgcacttt
    46201 gcgcatcttt cccccaagta aatggaccgt gtccgtgtaa aagtacccct ggaattgcta
    46261 agggatcaat tttgcgttct ttgaatgttt ctatgataac ttttcctgtt tcatattcgt
    46321 acccgctgtc gatttcctgt tgtgtcaaaa atcgagcaca aggtaccgat ccataaaaag
    46381 tatctgcatg cgtcgtcccc atcgcaggaa catccaatcc tgcttgtgcc cagatcgtcg
    46441 cccatgtcga gtgagtatgg acgattcctc cgatttgagg aaatgaacga tataaaacag
    46501 catgagtagg cgtatccgaa gagggattga gtttaccttc aatgatatga ttgtccaagt
    46561 ccacaacgac catgtcgtct gctgtcatct cttcataact gactccgcta ggtttgataa
    46621 cgaaatatcg tgtttcagga tcaaaagcgc tcacatttcc ccatgtatat ttgacgagtc
    46681 cgtgtttagg caagtcaaga tttgcttggt atacttcttc tttcagttgt tccagcattt
    46741 tctatcactc ctttttcttg caattgatga atcgctgttt tttcaatgtc gatcccctca
    46801 cggtaacgtc tcataaatat ctgatagcct gctatttctt ctgctgaagg tgtgatggtg
    46861 attccttggt cttgtccaaa cacgtcttct gccaagaagt tttctaaaga cttcccatgt
    46921 cgatccatca tataggctgc taataacgcg attccccacg ctccaccttc tccggctgtg
    46981 tccatcactg taactggtgc ctccatagca gatgctagaa tccgctgtcc aacttctggt
    47041 gttttaaaaa tccccccatg ccccactaaa cgatcaattt ggacattctc agatttcaat
    47101 atatccattc cgattctcat tgcaccaaaa gcactggcta aatggatccg catgaaattc
    47161 gctaaatcaa aacggctacc tggtgtacgt acaaataatg gccttccttc atttactcct
    47221 gtaatgtttt caccggaaaa ataaccataa gaaagtaatc ccccgcaatc cgtatcgcct
    47281 tctagcgctt tgttgaataa taccgtgaat aatttatctc tgcttatctc catacccaaa
    47341 gaattggtaa attcttcaaa aatactgacc caagcattga tatccgaaga gcagttattc
    47401 gtatgaacca tgcctactag ttctcctgaa ggtgtagtga ccatgtcgat ttccggatgg
    47461 acattttcta gttctttttc taatacgatc atagcgaagg cggaagtacc agccgaaatg
    47521 ttccctgtcc ctttggctac actgttcgta gcgaccatac cggttcctgc atccccttct
    47581 ggcggacaaa ccgggattcc tggtctcaaa tttcccgtag ggtcgatcag ataagcccct
    47641 gattcgctta acttgccagc ttcttctcct gctactagaa ctttaggcaa aatgtgatcc
    47701 aagtcaatcg ctacgtgcgc tttttcgatt aactgaccaa aaatattttt catttcttga
    47761 tggtaggttt tcgttgaagg atcgatagga aacatgcctg atgcatctcc tacgcctagt
    47821 actttctcac cagtaagctg ccaatggaca taacctgcaa gcgttgtcaa aaaattaatt
    47881 tctttcacat gttcttcttt attcaaaata gattgataca aatgagcgat actccatctc
    47941 tgcggaatat tgtattgaaa tttttccgtt aatattcgtt ctgcttcttc agtgattgta
    48001 tttcgccaag tacgaaatgg tactagtaac tgttccttgc tatcaaatgc tagataaccg
    48061 tgcatcattg cactgaaccc aatcgacccg attgtcgtaa gattctcgcc atagatttcg
    48121 aatacttctg ctgccatttt tcggtaactg acgcgcagtc ctttccaaat atcttctaat
    48181 gaataggtcc agataccatt ttctagtttg ttttcccact cataactacc agcggcaata
    48241 ggagtaaaag atgaatcaat caatacagct ttgatcctag ttgatccaaa ctcaatccct
    48301 aaagatgtac gaccatctat gacatctaac atactttgcg catttacttc cttcataatg
    48361 atttaactcc ctccttctcg tcaaacctga tcaaaccgtc tttttagtag cgcttacaat
    48421 gattattgta tacttttgta cgtacatgtc aagcagttat ttacaagaat acaataaaaa
    48481 acacttgttt tgtacgtaca taagttataa aatgaaattg cttacaaaat ctgatcaacc
    48541 gccttctcga aattctagaa ggagagaata cttatgaaaa aagaaaaaaa agtttcctca
    48601 tcgtttattt atttttttgg cgcatttggc ggcattctat ttggatatga tattggtgtt
    48661 atgaccggcg ccttaccttt tttacagcat gattggggtt tggcaggtaa agcttctttg
    48721 ataggatgga tcacgtcttc ggtcatgtta ggtgcgatcc ttggcggctc tttatctggt
    48781 cttctttctg ataagcttgg acgacgaaaa atgattcttc tttctgcact tatttttatg
    48841 gctggttctg tcctttctgc atctgcccct cacaatggtt cttattttct gattgcagca
    48901 aggattcttc taggtttagc tgttggcgct gcatctgccc tagttcctgc ttatatgtct
    48961 gaaatggctc ctgctagact aaggggaaga ttgtcaggga tcaatcaagt gatgattgct
    49021 tctggcatgc tcctttccta tgttgcagat tatttgctaa aagggttgcc tgaaacgatg
    49081 gcttggcgcg tgatgttagg gctcgctgct gtccccgcgc tgattttatt tttcggtgtt
    49141 ttagctctac cagagtcacc gcgttttctt atgcaatctg gccgcttgga agaggcaaaa
    49201 agagtattga actatattcg gacaccaaaa gaagctgaac aggaattcga acaaatccag
    49261 ctgaatgtca aacaagaaaa gacaaccgga acttcttggc atactttgtt tttagaaaaa
    49321 tatcgttcct tagtcattgc cggtattggt gtagcagtat tccagcaatt tcaaggagct
    49381 aatgctattt tttactatat tccgctgatc gtagaaaaag cgactgggca tgcagcgagc
    49441 gatgcattga tgtggccgat catccaagga atcattttag ttgctggttc tttgctcttt
    49501 ttggttattg cagacaagtt caatcggcga actttattga aaataggcgg tagcgtgatg
    49561 ggactttctt tcatcctacc agctgtttta ggaactgtac tggatgctca tacaaattcg
    49621 ttgttgattc tcttgttttt atgtatttat gtcgcatttt attcttgcac atgggctcca
    49681 ctgacatggg taatcgtagg cgaaatattc ccactagctg tccgcgggcg agcatctggc
    49741 ctagcctctt cttttaattg gattggttct ttcctagttg gcttgctttt tcctgtgatg
    49801 actgcttcta tgtctcaaga aatcgttttc ggcatttttg ggatcatctg ctttttaggg
    49861 gttttattta ttcaagagat cgtaccagaa actcgtggga aatctttaga ggaaatcgaa
    49921 caaagtgcat ctaaaaaaac ttatcctaaa cgtatttcta tccatcattc ttaaagaggt
    49981 tgtgacagaa gtggtcagct tcaagaaata agaaggaacc gcttagtttc cagtcacaag
    50041 gaacaatttc aaaaaacagc ttctcatgtt tgatgaatat tgtgacttgt gaccagttgg
    50101 taggagaaca ccgtttagtg gagaggtggt gaaagaaacg ttcagccttg agcgatgaca
    50161 aaagctctca caaacattgc gacgagtaac cgtaggagca ataagaggcg gtaaaaaccc
    50221 gtctcttatt tttcgttttc tttcactgtt tcccgttcaa ctagtacagg ttcatagaga
    50281 atatttggct gtgcctgtcc tttttcaatc gttgtgacga tccaccgtgc ggcatcttgt
    50341 cccaactttt cttttggatg cgtcaatgtt gtcaattttt tattccctaa atggcttaaa
    50401 gaagaatcgt ctgttcctac gatcgatagg tcatctggaa tatgatagcc attttctgtc
    50461 aacttatcta gcattaaact agctatttga tcgttatagc aaatgatgcc atctaaagct
    50521 tgttcttctt tcaaccgttg tactgttttt tcagcgattg tttctcgtgt ttccgtggtg
    50581 aaagtaatga tatcttccgt caaaaaacgc acattatact gttcacacgc gctgataaaa
    50641 cctttcatcc tgtattttcc ttgaagatcg tcgatttttg tgatgaacaa cagacgctga
    50701 tgtccttttt ttatcaaata ttctgctgcc ataaaaccga ctttcacatc atctaaacaa
    50761 atataagagg catttaactc ttcataagca gcattgatca tcaccatcgg aatgccttgt
    50821 tctctcaaag caacataaag tgctaagttt gggttatact gattactttt cgtaggctca
    50881 acgatcaaac catctacccc ataagcaatc attttcttca aacactcttt ctccaattcg
    50941 tgatcgttat tcgtactggc taaaagcaat gaatacccag attctcgtaa ttcattttcg
    51001 attcctcgga taatcgacgg aaaaatataa tcagagagat atgtggtgat gacaccgatc
    51061 gtttttgttc tgttcaatgg tgttttttct ttcttatagc tgtcatctac atatgtgccc
    51121 gacccttttt ctttacgcag atagccctca tttaccaaca cagcgattgc ttgccgaatc
    51181 gtatgtctgc tgacgtcata tatttcttgc agcttgaatt ctgagggaat ggttgtattt
    51241 acttcgtatt cctcacgaag gatcttttgt tttatctctt ctgcaatatc ttgatatttt
    51301 gccatttgcc ttgccccttt ttatttgtac gtacatatat cataacgagt ctgcaaggtt
    51361 tattcaagca aaaatcaagg tttcttgtgc tcataaggca tacggaacag gttcaccaaa
    51421 ttctggaaat ccttgctcat tccacgtaaa gcattgcgcg ttcgcgtgcc gattaggatt
    51481 gtctaacgga tccccttttt ggttcttctc tggtcgagca tgatagatca aaatatcaaa
    51541 gcacccgtct tctgaaaccg taaacgagtt gtgaccagga ccaaattgct tatttttttc
    51601 agaagaaacg aatactggct ccgataattt atgccatgat tcttttaata aaagattctt
    51661 gtgctcatca gcccacagca ggcccatggc atagttttca tctgttgcac taccagaata
    51721 cgtaatgaat attttcccat tgcgagtaag gactgctgga ccttcattta ctgaaaaacc
    51781 ccgtttttcc cattcatact caggtataga aaggaggatc tgcttcccct ttaacgtcca
    51841 aggattatcc atttcggata gataaagatt agaattccca gtaatttcag gatcttgttg
    51901 ggcccacaca taatatagct ttccttgaga agagaaagtt gttccgtcta aactgaaagt
    51961 ttcccttttc gtttttattt gtcccttttc tatccaatct gtattcattg gatctatatg
    52021 ctgattttca agtacgaaca tgcggtgatg atgagattca tcacgtatag ttttatgatt
    52081 gcttgcagca aagtaaatat accattttcc attgatataa tggacttcag gtgcccagat
    52141 caattcactc atcggtcctt cttcatgagc ctgccataca atcaggcttg cagcatgttc
    52201 taattcattc agtgactttg cccttcttaa ttcaatcact tggtagcctg gaaccgatcc
    52261 tgtaaaataa taataaccat ctgtatgttt atatacccaa ggatctgccc gttgccgaac
    52321 gataggatta ttataaagtg tcttcatcat aacaaatttc ctcacatttt ctttattcta
    52381 attacattcc aagagtattt ttctatccct acatttaatt cgtcatgttt ttctattgaa
    52441 aaatagttta tttcaggttt tattttttct ggttcttcac gtgtattttt gtccaacaga
    52501 tcgctactgg ttaatgtgta gtgctctcct tgatctagca aattgaaatc atgttcaatc
    52561 gttatctttt gttgttcatt tgtgcgattg atcaagaaaa tagtcagctg attgccgtta
    52621 tctacgatca cactgtccac ataaggaaca ttttttatct tttcacaatc ataaacaggg
    52681 caaatgattt gttgtttcaa tacttgacct ttagcatatt tcgataagtg ttgcatcaca
    52741 taataagttg gattcctcca tgcttgtcct ttagcatctg tcagtattaa aggtattgtg
    52801 tttactaaca atgattgaca actaatcttt acgcgatctg cgtgtcgtaa aatagccagc
    52861 cctaatgaac caaataataa tgtatcttcc atagtaaagt aacaccaatc gatgggacta
    52921 ccgacttcaa aatccctatg tcttttttcc ggttgggcat gtacattcca ttcatcaaac
    52981 gctagataca ttgttttctc acttctcaaa tatcctttta cataatcaca agtagcaacg
    53041 atttcttcaa tttgacgatc gaatcgataa gcttttgcaa ggtatgtcgc tgtatcatca
    53101 cgctcatttt taggaggttt agtcaagtct tcttcatcat acttatcaat atagttatgc
    53161 aaagctaaat aatctacatt ctcataagct tcttctaatg cgatacgatc ccattcaggg
    53221 tacgatggta aacgtggcgt agagcttcca actaagattg tttcaattga atcatccact
    53281 aacttcatga cttttgaggc ttcatttgct aacctaccat attcataagc actcttagcc
    53341 cccacttgcc atggaccgtc tagctcattc cctaaacacc ataatttaac gccaaaaggc
    53401 tttttttgcc cattttttat tcttaaatca ctccagaagg ttccttttgg aaaattacaa
    53461 tattctacta aattttttgc ttcatcaatt ccttttgttc ctaaattcac cgtgaatatc
    53521 ggtttcgctt gtacatgact gatccattta aaaaattcgt ggagtccaaa agaatttggt
    53581 tctatacctc gccatgctaa atcaattttt tttggtcgtt gttctttcct acctacagta
    53641 tccacccaat cataaccaga tgtaaagttt cctccaggat agcgaatgac acctaattct
    53701 aattccttta cataagagag gacatcttgt ctaaacccgt caccatcagc tgtttcatgg
    53761 cccggttcat aaatccctgt atagacgaca cttcccatat gctcaacaaa tgagccaaac
    53821 agccgagaac tgatctctcc taccgtaaaa tcctctctga tttttacttt cacttcttcg
    53881 tccaacagct ttccctacct ttcatttcct atagtcaaga taatagatac aagtttccat
    53941 acgccaccag caaagataaa aataaaagga gggaaaagaa atatgagcag tacaaatcct
    54001 aataacaata cgcaacaaag aatatttctc aaaaaatcaa aaaatacttt ccatgcactt
    54061 tgttcaaaaa tttgtaaaaa tgtgatatct ttgttttcag aaatcacata tatccaggat
    54121 aaaagaaaca tggaataaat aagtaaaact acatatccac taattgaaag tacgattgtc
    54181 tgttcttcta ataattgatt aacaagaaaa taaataatga tcgttaacag actaaggaac
    54241 aagttcttac caaaatgttg ctttattcct tgaaagaaaa aataaatgat atttcctgat
    54301 ccctcacctt tccattgcgc cattacataa aaaacagcac atgtagcagg cacgatagta
    54361 ataacaggca aacacacgat tacccagaga atacctaata agatgtaatc agaaaccaca
    54421 atcaactgtc cataaagttt agtctcaact attttcatct tcctcactct ttcattccag
    54481 acatagctgt tccttgaaca aaatatttct gtgcaaaaat ataaaccact agtaatggca
    54541 gcattgaaat aaccgttcca gccattacag gtccataaaa agaaatgttc tgccctgtaa
    54601 ataaagctaa ccctgctggt aaagttctca tttttgaact acttgtcaat atcaatggat
    54661 aaagcaagtc attccaacta ttcatcagcg tgaaaatccc taatgataaa agagtaggtt
    54721 tgctcaatgg caacatgatt ttcaaaaaaa tattaaactc acttaatcca tcaatgcgtg
    54781 ctgcctcttc caaagaatct ggtaaagtaa taaaaaagga acgcatcatg aagataccaa
    54841 atgcagttgt catacgagga atgatcaaac ctgcataagt atcaagcatg ccaagcaaat
    54901 tgacttcaat aaatagagga atcatgaaga cttggaatgg gatcatcatt gtcagcaaaa
    54961 cgaaatagaa caaaactgat tttcctttga aacgcatacg agcaaatgca taccccgcaa
    55021 gagaatcaaa gaatacagaa gtgatcactg tccctccagc aaaaatcact gtatttttga
    55081 tgtaatctaa taaagggatc gttgtccaaa ccttaatata gttatctagg gtatagctat
    55141 ctgcaaatat tctaggcgga aatgagataa tgtctttttc gtgtttaaaa gaagaaataa
    55201 tcagccacaa aaaaggaaac gcaataacca acaatataac tagcatacta atcgttcgaa
    55261 tgattttttt accactaact gcttttcgac tattcacagt aactgtaatc gagctttctg
    55321 gtgccataca ggtttccctc ctatttcatt tgtttttctt tccgtgacat gtacacatta
    55381 gtggagatag ctatagttgc aacaatgata aacaaaaccg aagacagtgc tgatgcatag
    55441 cccaattcat aaggtgctgt aaatcctcga ctgtaaatat attgaacagc tgtttctgtc
    55501 ttaaattgtg ggccaccagc ggttgcaaca tatacttggt caaacacttg catcgaacca
    55561 atcagattcg ttaaaataca aaaacctaag gagggaacga tctgaggtag cgtaatgtta
    55621 aaaaactgct gtcgatttgt tgcaccatcc atttgagcag attcatacaa agagggagag
    55681 attccctgaa tagcagtcaa gagaatcgtc atagtaacac caaaattctt ccaaacagtc
    55741 atgatggcta ccgtcggcat agcaagggtt ggatcgcgaa aaaattgagg gatcggcaag
    55801 ccgacttgtg aaaacaagta aggaatcgcc cctacattcg gatcaagcaa catagaaaaa
    55861 ataatcgcaa ttgacgtcaa tgaacaaata actggaatgt aaaatgtcga acgacaaaaa
    55921 cgattgaatc tggtattttt agataatgct actgctgcta ataaaccaac gataatttgt
    55981 gttggcgttt cgagtacggt gaaatataaa gaatgaatca acgaattaac tgctcgttca
    56041 tcactaaata tacgtgtgaa attttcaaaa cttacaaaat ttcttgatgt aaagaaaata
    56101 ttcaaatctg taaaactaat agcaaatgtt cctaaaagcg gtatgacagt aaataataat
    56161 aaaactaaaa aagatggagt taaaaacaaa tacgcagcac gatcaggacg attccaatat
    56221 ttggtgaatc cgttcatata gtccacctca tctttcattt tgataaaata tacaatcttg
    56281 gtttaaacga atctgaataa gatatccgaa caatcgctgt gatttttgca ataacaccta
    56341 tctgcaaata ctaggtcgat cattcagata tctgtatctt gccaacagtc tatcctgtat
    56401 aaagttctgt actatttttg cagtaattga ttgatggcat catctgcatc gttcatgagt
    56461 gtctcaggat cttctcctgc taataatttt tcaatcagtg gattgatgat atcgttattg
    56521 atcgcagaga tttgggtaaa acctggtaaa tatggctctg catagtccat ttgtttcgat
    56581 aattcagata caataggatc attttttact tcttcatctt ttgctactga atccaagtag
    56641 gcaggaaaac cattttcgat actccatttt ttaccaattt cagttgtatt ccaatatttc
    56701 aaaaactcat agacagcttc ttttttatta ttatctgtac ttgccggaat ggcatatcct
    56761 acgccatcta aaatagctgt ttttttgtct gaatcactcc cttgtggtaa ggtagtcaca
    56821 ttataatcaa tctcgtttgc tttcaatccg ttatttaacc atggcccatt gatttcaatg
    56881 gctagttgac cagcattcat taaattgtcc atttcggcgc cagaaatcga ttctggacca
    56941 gtcttgtcta catggatcat tttttggatc gcttttaacg ttttaaggtt ctcaggagac
    57001 gcaaattcag acttagtata atcatcgttc actaatttac cgccatttgc taacatccag
    57061 ttatataacg gtgcagttcc atccttattg aagacaaaac ctgatacatt tttactacta
    57121 tcagttaatt ttggtgccat ttctgtcaat tcttcccaag tagtaggtgg ttgttcagta
    57181 tctaatccag cttgtttaaa caaatcatta ttccaataca tatacatgcc ttgtacctgc
    57241 atggggacaa aatactgttt accttctact tcacctaatt caatagccgt ttctttaaaa
    57301 tcagatttat ctactccatc atattcccag aaatcgtcta atggttgtac cgctccgttt
    57361 tctacatact gtgcaaaatc tggataattt aatgcaataa agtcaggtgc ttttttagaa
    57421 gaaatcgctg taggaagttt ttcattcaaa ttggcccaag tcatgatatc catttcaatt
    57481 ttgatgtttt tatcatttgt tttattaaat tcattaacga tttcttgcaa tatttcacca
    57541 tcagatgcag taaacccatt ccaaaaagtc aaagtcactt ctttatcaga atcagattct
    57601 gctgaacttc cgccaccgca agctcctaaa atacctgttg cagctaccaa caaagagcca
    57661 attaaccatt ttttaccttt cattttttgt tcctccttca tctgataaac gacatctgac
    57721 cacattccaa gatagcggtt tcaataaaca attgattttc ccatcttcag cttttacatt
    57781 tgttgaatga cttggtttta cttgttcact tttaggaaga ttagttgctt ttgtatcaaa
    57841 tccactcatt tcagaaaaat caatcactcc ctctaatgat aattcagaca agtgaacagt
    57901 tagattcatt tcttcttctc cacggttaac tgcaaatatg acaagttctt tgtcatttat
    57961 tacggctata ctttctaaat acggcacttc ttcaaaatct tccgtcgcat agtttgctga
    58021 acgaatttgt gggcttaaca cctctccttg tccatacaca gcagcttgca taaatggata
    58081 atagatcgtc tgtctccaag cttctccgtt tgtttctgtc ctgattggtg caataacatt
    58141 taccaattga gcaagacaag caatctttac tctatctgca tgtttcaata aagtgattaa
    58201 tagacagcct aatagtaatg catcttcaaa attatagtta tcttctagta aaggaggagc
    58261 aatctgccaa ggcgctgctt gctcatcttg ttccgctgaa tgataccaaa tattccattc
    58321 atcaaaggac aggttgatct gcttcttact atgtttcttc gccttgactg tatcgcacat
    58381 cgctgtcact tcagaaatga accgatccat gtctaaggaa cgtgcaagat aatgagaaag
    58441 atcatttttt tggttgccat aatattgatg cagagacaaa taatctattt gatcgtacgc
    58501 ttgatcaaga actgttaact cccaatcacc aaatgtaggc atttgccgat tcgaacttcc
    58561 gcataaaacc acctcaattg aatcatctac gagtttcatc gccttagccg tttcctctgc
    58621 taaccgtcca tattcttccg ctgttttatg tccaatctgc caaggaccgt ccatttcatt
    58681 ccctaaacac catgtcttaa ttccaaaagg gctctcttga ccattcgaac gccttaaatc
    58741 actccaataa gttcctttag gaaaattaca atattcgact aaattacgag ctgcatctac
    58801 tccacgagta cccagattga cagccatatt gatttcggcc cccacatgct gacaccagtt
    58861 ataaaattca tgaatcccaa cttcattcgt ttcaattgtc cgccaagcta aatccaacct
    58921 tctaggtcta tttgctatgg gcccaatact gtcttcccaa cgaaatcctg acacaaaatt
    58981 tccaccagga tatctaatca tgggaatgtt caattctttt accaagtgaa tgacgtcttg
    59041 gcgaaatcca cttttattag ctgatggatg atctggctga taaatccctt catatactgc
    59101 tcgtcccata tgttcaataa atgaactgta caaccgcgga tcaactttgc ctacacgctt
    59161 gacactacta atcaacattg tggcttccat aactgacctc ctttaattta tacatgaata
    59221 atacacctcg ttgaaagcgt tgtcaataat ataaatgtta aacttactta ttttttatcg
    59281 attttaatta accaaaatat taataatgat taaaacaaat aatttaatat aacttttatt
    59341 aacaaatatg ttaatatata agaaaaacct tttaagaggt gatatatatg caactacaaa
    59401 taaacgaaga atccttaccg gtatatgaag cattagctag taaaacaagg atcaagatca
    59461 tccagctact ttccaaaaaa aagatgaatg tcaaagattt ggcaaaagaa cttggagtga
    59521 gcagtgctat cacgacgatg cacgtgaaaa agctagagga agcaaacatc atcaaaacag
    59581 aaaaagtagg acaacaaaaa atttcgagcc ttcgtgtcga taaaatcgat atcagttttc
    59641 cagaaaaaat attcaatgct tttgatacga aagaaacatc gatacctatt ggtcactata
    59701 caaattatgc aattgaacca acatgcggtt tagctacgat ccatgatttt atcgggaaag
    59761 tggatgaacc acgctatttt atggatcctc gaaggatgga tgcccgcatt ctctggttta
    59821 ccagcggatt tgtcgagtat caggcaccta attttttaaa tacagaggat acattggaga
    59881 tgttggaagt ttctgtggaa atttcttctg aatttccttt ttcaaatgac aattggcctt
    59941 cagatatcac tttcagccta aatggtgtgg agctaggaac atggacgagc cctggagatt
    60001 ttgctgatat ccgcggtaaa tatacgcctg attggtatcc agataatctt aatcaatacg
    60061 gcttgctgaa gacgatccga attaccaaac acttgacgaa tatgaatgga gaacccttgt
    60121 caaacatcac gatcaacgac atccctaaag agcaagacac ttggcatttg cgaatcgaag
    60181 tgaaagacga tgccaaacat gtggggggct gtactttatt tggtaaagga ttcggtaatt
    60241 acgatcagga tatcaaatta aaagtttact acagctgata tttttctttt ctattttata
    60301 aaacaaaaac agccaatccc tatgaaggga cggctgtttt tgctttattg ttatttccag
    60361 aaatcatcca aaacggaaat tggcaaatgt cgtttatgtt gtgtcttgtt ccaccatgct
    60421 tcgatttttt cttgtgcttg agcgggaact tcttttcctt ctagataatc atcaatttca
    60481 ttatatgtga cacctagtgc gacttcatct gcaatcattg gtttcccatc ttcaagatct
    60541 gcagtcggaa tttttgtgta taatttttcc ggtgcattca atgcttgaag caattgtttc
    60601 ccttgtcgtt tgtccaaacg gaataatggc aaaatgtcgg ctccaccatc accaaatttc
    60661 gtgaagaatc cagtaatgtt ttctgcagca tgatctgtcc ctaatacggc tcctgctctt
    60721 tctccagcta cggcatattg cgtaatcata cgttgacgtg ctttgatatt tcctttgtta
    60781 aaatcgctga ttcccacacc tgcttcgctt aatacttgta cttgtgcatc aacagctggt
    60841 ttgatattta ctcgcagact aacatcgggc tggataaagt caagagcagc ttttgcatct
    60901 tcttcatctg cttgttcacc ataaggaaga cgaacagcga taaattgata gttttcatct
    60961 tttgtttctt cacgcatttc ttccatcgtt agttgcgcca aacggcctgc taaagtagaa
    61021 tcttggccgc cgctgatacc tagtacaaaa gttttaagaa acggatattt tttcaaatat
    61081 gctttcataa agtcaatact tttacgtatt tcttcttttg gatcaattgt tggtttgaca
    61141 cctaattcat tgataatttc tgtctgcagg ctcatttatt ctcctcctat gcttctgtat
    61201 gaagttctgc tacgcttttt ttcattcgtt ccatgatagc cattttatgg ttccagcatt
    61261 cagtagacag atcgactggg tatttttgcg gattcaagtc tcgtttatac tcttcccata
    61321 gtgaatctag attttctttt gtatattctt tgatttcgtt tagtgcaggt aattcgtaaa
    61381 ctctttttcc ttcgataaag atatcttgta aaactgggcg agctgtaaag tctcgaacag
    61441 ttttattgat aaacgtatgc actggatgga acatgaagat agcttcttct tctcgagggt
    61501 cttcatccca taatgtcacg tagtctcctt cagatttccc atcaaaattt cgagtgatgc
    61561 gccacacttg ttttttccct ggagtagtta ctttttctgc attgcttgat aacttgatcg
    61621 tatccatcat ttttccttca tcatcttcga tcgatactag cttgaatact gcaccaaggg
    61681 ctggctgatc ataggctgtg atcaattttg tcccaacacc ccatacatca attttagctt
    61741 tttgcatctt taaattcaaa atcgttgctt catccaagtc attcgaagca tagatttttg
    61801 cttccgtaaa tccggctgca tctaattgtt cccgaacacg cttagatata taggccatat
    61861 ctccactgtc gatgcgtacg ccttggaagt tgattttgtc acctaattct ttcgccacgc
    61921 gaatcgcact tggcacacct gatttcaacg tatcataagt gtcgactaag aacacgcagt
    61981 ctttatgggt tttagcgtag gccataaatg cttcataatc atttccataa gattggacta
    62041 acgaatgagc atgggtcccg ctagctggta tcccaaatat cttcccagca cgtacattgc
    62101 ttgttgcatc tgctccgccg atatatgccg ctctagttcc ccagactgct gcatccagtt
    62161 cttgtgcccg tctagttcca aattccaata gtggatcttc tccgatgaca gatttgatac
    62221 gagctgcttt tgtcgcgatc aacgtttgga aattgaccat attcaacagt gctgtttcta
    62281 ctaattggca gtgcgctaaa ggcccttcca cttggattaa tggttcattt gcaaaaacta
    62341 gttctccttc acgagcagaa cgaactgttg ctttaaattc aaaattttgt aaatactcca
    62401 aaaaaccttc aggatagact tctaatccac gcaagtaagc aatatcggaa tcattgaacg
    62461 tcagattctc taaataattg actaaccgtt ccaatcctgc aaaaaccgcg tatccgtggt
    62521 taaaaggcat ttctcgaaag taacattcaa aaacagcatg taaatcagct cgacctaatt
    62581 cccagtatgt ctgcatcata ttgatttgat atagatccgt atgcagcgtc aaactgtcgt
    62641 ctgggtaagt attattcatc ttgtattact cctcttggca agttttcatt tcctacccat
    62701 tataacaaaa aaaattaata ggaaaagaaa tcttccattt tttctgaaaa agcacatgtt
    62761 ttacggatat ttcttgctaa aaatgaactc catctgctaa aactatcctt tcttattggt
    62821 atttaaccta aacgagaaag aatacttatt ttattcaatg tatccgagac acgaagaaat
    62881 tagcgttttc tatttttctt acttgataaa ataagtagat tttcctacta caatcgtagc
    62941 cgttaagata ccgatgattg aacaaatata accaaatgtc tctacaacat aatcaatggc
    63001 aaaagttttt gaagcaataa tacgtctttc cttttttgct tcttatcccc aaagaaacgg
    63061 atataactta ttatgtattg aatttttttg gaaaggcgaa ttatgacaag atataccttg
    63121 cgttgaaaaa actcgcggaa aaaatatcac acgtttttaa aaagatacaa agtaagaaag
    63181 cagctgtcaa tagaatggaa acaaaaatgg cgagacatca atcagtctcg ccttgttttt
    63241 tattgtatac attagatatg gagcaggtta ccataaagat gattattagt cgttattaaa
    63301 tagaatcctt cgtatttttt tgttctatca ctacaatatc tttatttgga cgtttgaatt
    63361 gtattccggc acgttctagc tcctgtttca ctcctacatg gactttgaca tcttctcgtg
    63421 tcatttttaa aacgtgatga atcgcatagt cgatttcttg ccttgtgatt tttttcttcg
    63481 tattgatcat cacgacttgc tttttatcat atgtgtctac caatagcagt tgtgttcgat
    63541 ctaacgaaaa taatttgtaa taattaatag ccgtattccc aaaaaagaat tccactactt
    63601 taggataaaa tgtcttaata tttaaattac tattcacagg tgtttcgatt agcttcatct
    63661 cattcctctt ttctatatag tcgatttaga tccaatctta agttatgtta ccatctttcc
    63721 tagcattaag gaatcggtta caattgaacc tttaaagaca aaaggattta ccttttatca
    63781 cttaatagta taacataatt tcgtgagaaa aaaatacaat tttttcttga ccatctcgtt
    63841 acgtaaagga ttatgcttac atttgtcagg aggaaaaata atggagtaca caataaagaa
    63901 aatgtcagag atatcaggag tcagtccgag aaccttgcgc ttttatgatg agattggact
    63961 gctaaaacca gctaggatca attcttcagg ttatcggatt tatggcaaaa aagaagtcga
    64021 tcgtctgcaa cacatcttat tttatcgaac aatggcattc aagttggatg atatccaaga
    64081 ggtcttagac aatccatctt ttgaccacca gaaagcttta atcaagcatc agcaaatgtt
    64141 gctggaaaaa cgggcacaaa tcgataccct cttgacaact gtccaacaga cacttgatat
    64201 gtacgaagga ggaagaaaaa tgagtgacaa agaaaaattt gaagggttta aacaacaaaa
    64261 gttgaaagaa aatgaagaat cctatggaca agagattcga caaaaatacg gaaaaaaaaa
    64321 ggttacagca tccaatgaaa aatggatgaa tatggataaa gaaacgtatg aagaaatgca
    64381 aactgttgaa aagcagatgt tatttgatct cactctttac ttaaaagaac cagaagatga
    64441 aagcttagca gaccgtatct tccaagctca taagaagtgg ttctcttaca gctggcctga
    64501 atatcagcca aaagcgcata aaggactagg aatgatgtac gaagccgatg aacggttcac
    64561 tgcatattat gacgagcgaa gtggtcatgg tgcagcaaaa gctttaaatg aaattatcca
    64621 aaaaaaggcg taatctttta catgatccaa caaaaaagta cacgtaaaat gacctaataa
    64681 caaaacagca gacacccaag ttagattttt atgctaactt ttggtgtctg ctccattctt
    64741 tcacaagttt tttcatccta ctttaaatat attcatcgtt gaataaacgt ataaggtatt
    64801 ttcactttat cctttttttc tgtaagaaac aattgaaatg ccatttctcc gcaagcttcc
    64861 agatggtggt cgatggttgg gaaaccaaga agctcgctga tcagcaaatt atcgcgtcca
    64921 atcaccactg gtgtcttatc ttgcagatag ctttgcaaga tgcctgctgc aaccatatcc
    64981 gaatttgtaa agacaccatc aactccaagc tctttaaaaa agaagcctgc ttgttttcct
    65041 ttctctgcat ctatacaatc ccaaaaaaca agtgattcat caaaagatgg aaaaagttga
    65101 tgacatagct gcaaagtgat ttttgagtta taacttagcc tgccacttcg ccccaacgtg
    65161 atgcccaatt gctttatacc ttgctgcttc aaataagtaa gaccttctgt aattgattgt
    65221 tctcgatcaa taaagacaca gcttgcctgt tttcccttga tttcttcaca aaaaactaga
    65281 ggtccatatt tttgatatgc caataaatca tctagtgtat tggcacgaga cgtaatgatc
    65341 aatccatcga aagctttagc tgcaaattct tcaagatacc ttcgctcttt ttctttatga
    65401 tagcccgtcg gtaaaagtgt cactttgtaa tcataatcaa acgctgcttc aatgatccca
    65461 ctgatcaact gatcaaaata cggatgattg gtataaggca gaatcactcc aatgcttttc
    65521 gtttttccat aacttaactg tctggcattt gcattaggga tataatcgac ttctttcatg
    65581 accgcaagta tttctttcct tttttcctca tctacatatg gatgtccatt gattacacgt
    65641 gaaatagtag aaactgaata acctgtgagc tttgcgatat ctcgaatgtt tgccactgct
    65701 gcttcgccac ctatacaaaa agattttctt gtaaatagtc taccacagct ggaccgcatt
    65761 caaaaaactg atacattttt ccttcatgcg aaaaactgat ccgctgcccg ccccaatata
    65821 tatcttttgt caaattttcg atttcattca ttggtatttc ttttactata ttcctattca
    65881 acccttgaaa actgtacaac gttaatacgg atcccttgaa ctcgacaaac gcataaaaat
    65941 aattgttcgt tgtgccatct gttatctcta cataagtatt cattgcaagc cctccttttg
    66001 atactttgag tatagtgtat gaaacgcgtc tcatagcaag gagtttgttt tgccaaagaa
    66061 aagttttatt cctctttcat tattaccaaa gtctcttatt ttaaattgaa tacattataa
    66121 tgatactgta aatatatctt tattattagt gaatg
//
LOCUS       DAZKSU010000007        60771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000007 DAZKSU010000000
VERSION     DAZKSU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.007"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..591)
                     /locus_tag="ACG8Z3_000459"
     CDS             complement(10..591)
                     /locus_tag="ACG8Z3_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0822807.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(724..1407)
                     /gene="rpiA"
                     /locus_tag="ACG8Z3_000460"
     CDS             complement(724..1407)
                     /gene="rpiA"
                     /locus_tag="ACG8Z3_000460"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0822808.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1726..2139
                     /gene="rpsL"
                     /locus_tag="ACG8Z3_000461"
     CDS             1726..2139
                     /gene="rpsL"
                     /locus_tag="ACG8Z3_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0822809.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2223..2693
                     /gene="rpsG"
                     /locus_tag="ACG8Z3_000462"
     CDS             2223..2693
                     /gene="rpsG"
                     /locus_tag="ACG8Z3_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0822810.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2787..4874
                     /gene="fusA"
                     /locus_tag="ACG8Z3_000463"
     CDS             2787..4874
                     /gene="fusA"
                     /locus_tag="ACG8Z3_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0822811.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLASYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5046..6233
                     /gene="tuf"
                     /locus_tag="ACG8Z3_000464"
     CDS             5046..6233
                     /gene="tuf"
                     /locus_tag="ACG8Z3_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0822812.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6640..6948
                     /gene="rpsJ"
                     /locus_tag="ACG8Z3_000465"
     CDS             6640..6948
                     /gene="rpsJ"
                     /locus_tag="ACG8Z3_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0822813.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            6979..7608
                     /gene="rplC"
                     /locus_tag="ACG8Z3_000466"
     CDS             6979..7608
                     /gene="rplC"
                     /locus_tag="ACG8Z3_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0822814.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7636..8259
                     /gene="rplD"
                     /locus_tag="ACG8Z3_000467"
     CDS             7636..8259
                     /gene="rplD"
                     /locus_tag="ACG8Z3_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0822815.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8259..8549
                     /gene="rplW"
                     /locus_tag="ACG8Z3_000468"
     CDS             8259..8549
                     /gene="rplW"
                     /locus_tag="ACG8Z3_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0822816.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8588..9421
                     /gene="rplB"
                     /locus_tag="ACG8Z3_000469"
     CDS             8588..9421
                     /gene="rplB"
                     /locus_tag="ACG8Z3_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0822817.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9464..9742
                     /gene="rpsS"
                     /locus_tag="ACG8Z3_000470"
     CDS             9464..9742
                     /gene="rpsS"
                     /locus_tag="ACG8Z3_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0822818.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9765..10112
                     /gene="rplV"
                     /locus_tag="ACG8Z3_000471"
     CDS             9765..10112
                     /gene="rplV"
                     /locus_tag="ACG8Z3_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0822819.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10126..10782
                     /gene="rpsC"
                     /locus_tag="ACG8Z3_000472"
     CDS             10126..10782
                     /gene="rpsC"
                     /locus_tag="ACG8Z3_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0822820.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10785..11219
                     /gene="rplP"
                     /locus_tag="ACG8Z3_000473"
     CDS             10785..11219
                     /gene="rplP"
                     /locus_tag="ACG8Z3_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0822821.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11209..11397
                     /gene="rpmC"
                     /locus_tag="ACG8Z3_000474"
     CDS             11209..11397
                     /gene="rpmC"
                     /locus_tag="ACG8Z3_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0822822.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11422..11688
                     /gene="rpsQ"
                     /locus_tag="ACG8Z3_000475"
     CDS             11422..11688
                     /gene="rpsQ"
                     /locus_tag="ACG8Z3_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0822823.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11746..12114
                     /gene="rplN"
                     /locus_tag="ACG8Z3_000476"
     CDS             11746..12114
                     /gene="rplN"
                     /locus_tag="ACG8Z3_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0822824.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12151..12459
                     /gene="rplX"
                     /locus_tag="ACG8Z3_000477"
     CDS             12151..12459
                     /gene="rplX"
                     /locus_tag="ACG8Z3_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0822825.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12487..13026
                     /gene="rplE"
                     /locus_tag="ACG8Z3_000478"
     CDS             12487..13026
                     /gene="rplE"
                     /locus_tag="ACG8Z3_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0822826.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13045..13230
                     /locus_tag="ACG8Z3_000479"
     CDS             13045..13230
                     /locus_tag="ACG8Z3_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0822827.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13266..13664
                     /gene="rpsH"
                     /locus_tag="ACG8Z3_000480"
     CDS             13266..13664
                     /gene="rpsH"
                     /locus_tag="ACG8Z3_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0822828.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13696..14232
                     /gene="rplF"
                     /locus_tag="ACG8Z3_000481"
     CDS             13696..14232
                     /gene="rplF"
                     /locus_tag="ACG8Z3_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0822829.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14393..14749
                     /gene="rplR"
                     /locus_tag="ACG8Z3_000482"
     CDS             14393..14749
                     /gene="rplR"
                     /locus_tag="ACG8Z3_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0822830.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14770..15270
                     /gene="rpsE"
                     /locus_tag="ACG8Z3_000483"
     CDS             14770..15270
                     /gene="rpsE"
                     /locus_tag="ACG8Z3_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0822831.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15285..15464
                     /gene="rpmD"
                     /locus_tag="ACG8Z3_000484"
     CDS             15285..15464
                     /gene="rpmD"
                     /locus_tag="ACG8Z3_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0822832.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15508..15948
                     /gene="rplO"
                     /locus_tag="ACG8Z3_000485"
     CDS             15508..15948
                     /gene="rplO"
                     /locus_tag="ACG8Z3_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0822833.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            15948..17243
                     /gene="secY"
                     /locus_tag="ACG8Z3_000486"
     CDS             15948..17243
                     /gene="secY"
                     /locus_tag="ACG8Z3_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0822834.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17305..17952
                     /locus_tag="ACG8Z3_000487"
     CDS             17305..17952
                     /locus_tag="ACG8Z3_000487"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0822835.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18143..18361
                     /gene="infA"
                     /locus_tag="ACG8Z3_000488"
     CDS             18143..18361
                     /gene="infA"
                     /locus_tag="ACG8Z3_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0822836.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18394..18510
                     /gene="rpmJ"
                     /locus_tag="ACG8Z3_000489"
     CDS             18394..18510
                     /gene="rpmJ"
                     /locus_tag="ACG8Z3_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0822837.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18528..18893
                     /gene="rpsM"
                     /locus_tag="ACG8Z3_000490"
     CDS             18528..18893
                     /gene="rpsM"
                     /locus_tag="ACG8Z3_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0822838.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            18921..19310
                     /gene="rpsK"
                     /locus_tag="ACG8Z3_000491"
     CDS             18921..19310
                     /gene="rpsK"
                     /locus_tag="ACG8Z3_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0822839.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19390..20328
                     /locus_tag="ACG8Z3_000492"
     CDS             19390..20328
                     /locus_tag="ACG8Z3_000492"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0822840.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20356..20736
                     /gene="rplQ"
                     /locus_tag="ACG8Z3_000493"
     CDS             20356..20736
                     /gene="rplQ"
                     /locus_tag="ACG8Z3_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0822841.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21008..21847
                     /locus_tag="ACG8Z3_000494"
     CDS             21008..21847
                     /locus_tag="ACG8Z3_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0822842.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21823..22692
                     /locus_tag="ACG8Z3_000495"
     CDS             21823..22692
                     /locus_tag="ACG8Z3_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0822843.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22682..23479
                     /locus_tag="ACG8Z3_000496"
     CDS             22682..23479
                     /locus_tag="ACG8Z3_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0822844.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23682..24428
                     /gene="truA"
                     /locus_tag="ACG8Z3_000497"
     CDS             23682..24428
                     /gene="truA"
                     /locus_tag="ACG8Z3_000497"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0822845.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24761..27430)
                     /locus_tag="ACG8Z3_000498"
     CDS             complement(24761..27430)
                     /locus_tag="ACG8Z3_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0822846.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(27668..27856)
                     /gene="rpmB"
                     /locus_tag="ACG8Z3_000499"
     CDS             complement(27668..27856)
                     /gene="rpmB"
                     /locus_tag="ACG8Z3_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0822847.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28126..28488
                     /locus_tag="ACG8Z3_000500"
     CDS             28126..28488
                     /locus_tag="ACG8Z3_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0822848.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            28540..30222
                     /locus_tag="ACG8Z3_000501"
     CDS             28540..30222
                     /locus_tag="ACG8Z3_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0822849.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            30339..32375
                     /gene="recG"
                     /locus_tag="ACG8Z3_000502"
     CDS             30339..32375
                     /gene="recG"
                     /locus_tag="ACG8Z3_000502"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0822850.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            32424..33425
                     /gene="plsX"
                     /locus_tag="ACG8Z3_000503"
     CDS             32424..33425
                     /gene="plsX"
                     /locus_tag="ACG8Z3_000503"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0822851.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            33547..33789
                     /gene="acpP"
                     /locus_tag="ACG8Z3_000504"
     CDS             33547..33789
                     /gene="acpP"
                     /locus_tag="ACG8Z3_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0822852.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34057..35061
                     /locus_tag="ACG8Z3_000505"
     CDS             34057..35061
                     /locus_tag="ACG8Z3_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822853.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35062..36006
                     /locus_tag="ACG8Z3_000506"
     CDS             35062..36006
                     /locus_tag="ACG8Z3_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822854.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36006..36968
                     /gene="opp4B"
                     /locus_tag="ACG8Z3_000507"
     CDS             36006..36968
                     /gene="opp4B"
                     /locus_tag="ACG8Z3_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0822855.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            36995..37909
                     /locus_tag="ACG8Z3_000508"
     CDS             36995..37909
                     /locus_tag="ACG8Z3_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822856.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            37935..39716
                     /locus_tag="ACG8Z3_000509"
     CDS             37935..39716
                     /locus_tag="ACG8Z3_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0822857.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40064..40750
                     /gene="rnc"
                     /locus_tag="ACG8Z3_000510"
     CDS             40064..40750
                     /gene="rnc"
                     /locus_tag="ACG8Z3_000510"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0822858.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            40770..44351
                     /gene="smc"
                     /locus_tag="ACG8Z3_000511"
     CDS             40770..44351
                     /gene="smc"
                     /locus_tag="ACG8Z3_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0822859.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            44360..45169
                     /locus_tag="ACG8Z3_000512"
     CDS             44360..45169
                     /locus_tag="ACG8Z3_000512"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0822860.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            45180..46178
                     /gene="ftsY"
                     /locus_tag="ACG8Z3_000513"
     CDS             45180..46178
                     /gene="ftsY"
                     /locus_tag="ACG8Z3_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0822861.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            46365..48632
                     /gene="gshAB"
                     /locus_tag="ACG8Z3_000514"
     CDS             46365..48632
                     /gene="gshAB"
                     /locus_tag="ACG8Z3_000514"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0822862.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(48721..49173)
                     /locus_tag="ACG8Z3_000515"
     CDS             complement(48721..49173)
                     /locus_tag="ACG8Z3_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0822863.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            49323..50273
                     /locus_tag="ACG8Z3_000516"
     CDS             49323..50273
                     /locus_tag="ACG8Z3_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0822864.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            50266..51225
                     /locus_tag="ACG8Z3_000517"
     CDS             50266..51225
                     /locus_tag="ACG8Z3_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0822865.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            51222..51977
                     /locus_tag="ACG8Z3_000518"
     CDS             51222..51977
                     /locus_tag="ACG8Z3_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822866.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSTLRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52000..52953
                     /locus_tag="ACG8Z3_000519"
     CDS             52000..52953
                     /locus_tag="ACG8Z3_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0822867.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(53476..53772)
                     /locus_tag="ACG8Z3_000520"
     CDS             complement(53476..53772)
                     /locus_tag="ACG8Z3_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0822868.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            54127..54483
                     /locus_tag="ACG8Z3_000521"
     CDS             54127..54483
                     /locus_tag="ACG8Z3_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0822869.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            54493..55392
                     /locus_tag="ACG8Z3_000522"
     CDS             54493..55392
                     /locus_tag="ACG8Z3_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0822870.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            55912..56640
                     /locus_tag="ACG8Z3_000523"
     CDS             55912..56640
                     /locus_tag="ACG8Z3_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0822871.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            56785..58812
                     /locus_tag="ACG8Z3_000524"
     CDS             56785..58812
                     /locus_tag="ACG8Z3_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0822872.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            58920..60017
                     /locus_tag="ACG8Z3_000525"
     CDS             58920..60017
                     /locus_tag="ACG8Z3_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0822873.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            60324..>60771
                     /locus_tag="ACG8Z3_000526"
     CDS             60324..>60771
                     /locus_tag="ACG8Z3_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0822874.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        20130 a        10866 c        12980 g        16795 t
ORIGIN      
        1 ttttaggtat tagatgcata attgctcgat ttctttttca ggtaaattga tatatttata
       61 acatgttcca actgaaacac cgcattttga agaaataaat tggatcgttt cttttttttc
      121 ataatagaga cgtcggattt ttttgatcgt ttttgcatcg atttttggtc ttccgccgat
      181 ttttcctttt ttacgagctt catctaatcc aactaatgta cgttctttga tcaatgcgca
      241 ctccatgttt gctaggtttt ccattaattg gaaatagatt tttcccattt cgcgcgtatc
      301 gattccttca tcaagactaa tcaagtgcag cccgcgatct ttgaataatt gcgtgagttc
      361 tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg caaatgacta gtgcatcgcc
      421 ttgttgcatg tcagcgatca ctgactccaa atgaacagca gtagaatcat tttgttccac
      481 ttcatatgtc tcatggaaaa tttcttgaca accaaagtca gataattgat caacctgagt
      541 tgttaaatca acgtcattta tggttgtgcg tgcataacca ataattttca tggtgattcc
      601 tttctagcag tgattttcta actggatata acagtaaaaa tgtagcataa agtacgtcaa
      661 aaagatagtt ttttcgcaaa ttttccgctg tcttttttta ttggatgatc aagcagtttt
      721 tctttattta cgacggattg cgtcaagaat ctcaacgcct ttttctgagc cgacaactac
      781 tttagaaacc atgtttagga aaagcccatg ttctactacc ccaacaagtt catctaaata
      841 ggtagctaag ctttctggct gttcgatgac ttctagatgc agatcaatga tgtaatgacc
      901 accatctgtg gttaagactt ctccgttttc gtctgtacgc aagacgggtt ggaatctttt
      961 ttcttcaaaa atatgaagta gctgttggga gccataaggg atcacttcaa ctgggagtgg
     1021 gaacttccca agtttatcga ctaatttaga agaatcaacg atccaaatcg tttcttttga
     1081 gtacgtagcg actattttct cgaaaagcaa agcagcgccg ccgcctttga tcccttgaaa
     1141 atcagcactg atttcatcag caccgtctat tgtcaaatcg actacgggga cttcatcaat
     1201 actctttaac ggtattccta atttttccgc ttgttctttt gtcgcatttg aagtagtaac
     1261 tcctgtgata cgtaaacctt cttcacgcat acggcgacca atctcttcta ccatataata
     1321 ggcggttgaa ccggtcccga gacctacgat catcccgtcc tttacgaatt tcgcggcttc
     1381 aatgccagct aattgtttta aattcatctt tgcgtccacc tttttttagt ttgagcatcg
     1441 tgctttcctc actattttag cgtgatttac ggtaaaaaga taccattctt ttttattcca
     1501 ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat gttttttcat tcgtgatatt
     1561 ctaacaaagg tgctttatct acaggtttct gtgaagaaac gtgctgactg tttatcaaag
     1621 catatttgat aaggatgcag acattgcatt tattttttat cacaaaaaaa gaaccacctg
     1681 gatgtgtgga actagacgca aatcgtggga aggaggaaac aaggaatgcc tacaattaac
     1741 caattagtac gtaaacctcg taaatcaaag gtggaaaaat ctaattcacc tgctttgaac
     1801 aaaggatata acagctttaa gaaaactcaa actaacgtaa actcacctca aaaacgtggg
     1861 gtagcaactc gtgtgggtac aatgacacct aaaaaaccga actcagcttt acgtaaatat
     1921 gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt atatcccagg gattggtcat
     1981 aacttacaag aacacagtgt tgtattatta cgcggtggtc gtgtaaaaga cttaccaggg
     2041 gtacgttacc atatcgttcg cggtgcttta gatactgctg gtgttaacga ccgtaaacaa
     2101 agccgttcta aatacggaac taaaaaacct aaagcttaat tttaattatt aaacaaatga
     2161 aacttgcttc tactatatag aagtaattac tatgaacaat atttcggaag gaggagttac
     2221 ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt accagatcct atttataact
     2281 caaaattagt aactcgcttg atcaaccgtg taatggttga cggaaaacgc gggattgcag
     2341 ctaacattat ctataatgcc tttgacttga tcaaagaatc tacaggtaac gatccattag
     2401 aagtgtttga acaagcaatg aaaaacgttc aacctgttct tgaagttaaa gcacgtcgtg
     2461 ttgggggttc aaactaccaa gtaccagttg aagttcgccc tgaacgccgc gttactctag
     2521 gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga acacacaatg gaagaacgct
     2581 tagcaaaaga aatcatggat gctgcaaaca acacaggtgc ttctgttaaa aaacgtgaag
     2641 acactcataa aatggctgat gccaaccgtg cgtttgctca ctatcgctgg taagattctc
     2701 tctgtttggt cggcttttat aagtcgacct cagatagtgt ccttattttt agcaactaga
     2761 tagtaaagag aggagaaaca aggagaatga ccagagaatt ttctttacaa aacactcgta
     2821 atatcggtat catggcccac atcgatgctg gtaaaacgac tgctactgaa cgtatcttgt
     2881 actatactgg taaaatccat aaaattggtg aaactcatga aggagcttca caaatggact
     2941 ggatggaaca agaacaagaa cgtggtatta cgatcacttc tgctgctact acagcacaat
     3001 ggaagggtca ccgaatcaac atcatcgaca caccaggaca cgtagacttc acagttgaag
     3061 ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt tcttgatgca caatcaggtg
     3121 ttgaacctca aactgaaaca gtttggcgtc aagcaacaac ttatggtgta cctcgtatcg
     3181 tatttgcaaa caaaatggac aagatcggtg ctgacttctt atattcagta tcaactttac
     3241 atgaccgttt gcaagcaaac gctcacccaa tccaattacc aattggtgcg gaagacgact
     3301 tcacaggaat tatcgacctt gtaacaatga aggctgaaat gtatacaaac gatcttggaa
     3361 cagagatcga agaaacagaa attcctgaag aataccgcga attagctgaa gaatggcgcg
     3421 aaaaattggt tgaagctgta gctgaaactg atgaagaact tactttgaaa tacttagaag
     3481 gtgaagaaat cactgaagct gagttaaaag aaggtatccg ccgcgcaaca gttaacgtag
     3541 aattctaccc agtactatgt ggttcagcat ttaagaacaa aggggttcaa ttactattgg
     3601 acgctgttct tgattacttg ccatcaccgt tagatatccc agctatcaaa gggattgacc
     3661 ctaaaactga tgaagaagta gaacgtcctg cagacgacag cgctcctttc tcagctttag
     3721 catttaaagt tatgactgac ccattcgtag gtcgtttaac tttcttccgt gtgtactctg
     3781 gtgttttaca atcaggttca tacgtacaaa acgcaacaaa aggcaaacgt gaacgtgttg
     3841 gtcgtatcct acagatgcat gccaactctc gttcagaaat ctctgaagtt tacgctggag
     3901 atatcgctgc tgctgttggt ttgaaagaca caactactgg ggacacacta tgtgatgaaa
     3961 agagtctagt tattcttgaa tccatggaat tcccagaacc agttatccaa gtcgctattg
     4021 aacctaaatc aaaagctgac caagataaaa tgggtgtggc tttacaaaaa ctatctgaag
     4081 aagatcctac tttccgtgct gaaacaaacg tggaaacagg cgaaacaatc atcgctggta
     4141 tgggtgaact tcacttagat atcatcgttg accgtatgcg tcgtgaattc aaagttgaag
     4201 ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt ccgtgctggc actcaagccg
     4261 aaggtaagtt tgtacgtcag tctggtggta aaggtcaata cggtcacgta tggattgaat
     4321 ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa tgcgatcgtc ggtggggttg
     4381 ttcctcgtga atacattcca gctgttgaaa ctggtttgaa agatgctatg gaaaatggtg
     4441 tcttagctag ctatccatta gtagatatca aagctaaact ttacgatggt tcataccatg
     4501 atgtcgattc aaatgaaact gccttccgtg ttgctgcttc tatggcatta cgtgcagcag
     4561 ctaagaaagc taatccagta atccttgaac caatcatggc tgtagaagtt gttatccctg
     4621 aagattattt aggtgatgtt atgggtcacg taactgctcg tcgtggacgt gttgaaggta
     4681 tggaagctcg cgctggcggc caacaagttg ttcgtgcgat ggttccactt gctgaaatgt
     4741 ttggttatgc tacaactctt cgttctgcta ctcaaggtcg tggtacattt acaatgacat
     4801 ttgaccacta cgaagatgta ccaaaatcag tacaagaaga aatcattaag aaaaacggcg
     4861 gaaaagctga ataatttctt aaaaccggat agtcctgcaa agagattatc caaagagact
     4921 atatttttaa tatagtttcg tgtaaaataa ttatcgatga tatgggcatg tggtgtacct
     4981 tgtgtacatc atatacctat cagacaaata aaaaatatat tgtattttta ggaggacatt
     5041 ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca cgttaacatt ggtactatcg
     5101 gacacgttga ccatggtaaa actacattaa cagctgcaat cacaactgta ctatctaaga
     5161 aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg tgctccagaa gaacgcgaac
     5221 gcggaatcac aatctcaaca gctcacgttg agtatgaaac agatacacgc cactacgctc
     5281 acgttgactg cccaggacac gcggattacg ttaaaaacat gatcactggt gctgctcaaa
     5341 tggacggagc tatcttggta gtttctgctg ctgacggccc aatgcctcaa actcgtgaac
     5401 acatcctatt gtctcgtcaa gttggtgttc cttacatcgt tgtattcttg aacaaagtag
     5461 acatggttga tgacgaagaa ttactagaat tagttgaaat ggaagttcgt gacctattaa
     5521 cagaatacga attccctggt gacgatgttc ctgtagttgc tggatcagct ttgaaagctc
     5581 tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt aatggctgca gttgacgaat
     5641 acatcccaac tccagaacgt gacaacgaca aaccattcat gatgccagtt gaagacgtgt
     5701 tctcaattac tggacgtggt actgttgcta caggtcgtgt tgaacgtgga caagttcgcg
     5761 ttggtgacga agttgaagtt gttggtattg ctgaagaaac ttcaaaaaca acagttactg
     5821 gtgttgaaat gttccgtaaa ttgttagact acgctgaagc tggagacaac attggtgctt
     5881 tactacgtgg tgttgcacgt gaagacatcc aacgtggaca agttttagct aaaccaggta
     5941 caatcacacc tcatacaaaa ttctctgcag aagtatacgt gttgacaaaa gaagaaggtg
     6001 gacgtcatac tccattcttc actaactacc gtccacaatt ctacttccgt acaactgacg
     6061 taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt tatgcctggc gacaacgtaa
     6121 caatggaagt tgaattaatc cacccaatcg ctatcgaaaa cggtactaaa ttctcaatcc
     6181 gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga aatcaaagct taattcactt
     6241 aagttttaaa gtaaagcaac atttattcgg acgtaatcgc tcggaacgaa ggtcttagga
     6301 tctttgttcc gagctttttt atttattgca ggatcaggaa aaaagacagg agtattataa
     6361 atagaagaaa cacgaaataa aagagtcagt attttctaaa tgaaacttta tgaaaacaaa
     6421 atgtttttta caagaaaaag ccttgctttg tgagtttata tatattataa tgacaaaggt
     6481 gcgtttactt tagtgaattg cacagacgta atcagaatga tttcgtcggc taagaaacga
     6541 gaggttgcga cacgcccgga cgctttgcca cgaacgagcg tgacggaaat tttcgtggag
     6601 ctatgtctac ttttcaaaat aggcgaagga gggaacaaga tggcaaaaca aaaaattcgt
     6661 atccgtttaa aagcgtatga acaccgtatt ttagatcaat cagcggataa aatcgtggaa
     6721 actgcaaaaa gaactggagc tagcgtgtct ggtccaattc cattaccaac tgaacgcagt
     6781 ctttacacaa tcatccgtgc gactcataaa tacaaagatt cacgcgaaca attcgaaatg
     6841 cgtacacaca aacgtcttat cgacattgtg aacccaacac caaaaactgt tgatgctttg
     6901 atgaagctag acttaccatc tggtgtaaac atcgaaatca aattataaaa ctaaaacaaa
     6961 aataatggag gtgtactcat gaccaaagga atcttaggga aaaaagtggg aatgacacaa
     7021 atcttcactg aatctggtga attaattcca gtaactgttg tagaagctac tccaaacgta
     7081 gtactacaag tgaaaacaat ggaaacggat ggctacgaag ctattcaagt tggttaccaa
     7141 gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc atgttgcaaa agcaaacacg
     7201 gctcctaagc gcttcattcg tgaattcaaa aatgttgagc taggagaata tgaagtagga
     7261 aaagaaatta cagtagatgt tttccaagca ggagacatta ttgatgtaac aggtactacg
     7321 aaaggtaaag gattccaagg ggttatcaaa cgccacggac aaagccgcgg accaatggct
     7381 cacggttctc gttaccatcg tcgtcctggg tcaatgggtc cagttgcacc taaccgtgta
     7441 tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc gcgtaactat ccaaaacttg
     7501 gaagttgtac gtgtcgatgc agaaagaaat gttatcttga tcaaaggtaa tgttcctgga
     7561 gctaaaaaat ctttaattac tatcaaatca gctgtgaaag ctaaataatt gtaggaagaa
     7621 aggaggaact aaggaatgcc gaatgtagca ttattcaaac aagatggaag ccaaaacggt
     7681 gaaatcacac ttaacgaaga aatcttcgga attgaaccta atgaaagtgt tgtttacgat
     7741 gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac acgcggttaa aaaccgtagc
     7801 gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag gaacaggtcg tgcacgtcaa
     7861 ggatctatcc gctcaccaca atggcgtgga ggtggcgtag tctttggacc aacaccacgt
     7921 tcttacagct acaaacttcc taaaaaagtt cgtcgtttag caatgaaatc tgtattgtca
     7981 gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct tgaacttcga cgcaccaaaa
     8041 acaaaagaat tcaaacaagt tcttgctaac ttatctatcg actctaaagt attggttgtt
     8101 ctagaaaaag gtaacgactt tgctgcttta tcagcacgta acttaccaaa cgtttctgta
     8161 gtaacttctg ataatgtgag tgtgctagac gttgtatcaa atacaaaagt gttagcaaca
     8221 caaactgctc ttactcaaat tgaggaggtg cttgcataat gaacttacta gacgtaatca
     8281 aacgcccagt gatcactgaa aaatcaatgc ttgccatgga tgacaagaaa tacacttttg
     8341 aagtggacac tcgcgcaaac aaaactttag taaaacaagc ggtagaagct gcttttgacg
     8401 ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa attcaaacgt atgggcaaat
     8461 atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac attgactgaa gattcaaaag
     8521 aaatccaatt attcgaagct gctgaataag cactaataga taactcatta aattaggagg
     8581 gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat ggtcgtcgta acatgacagg
     8641 ttctgatttt gctgaaatca caacatcaac acctgaaaaa tcattgttac aaccattgaa
     8701 aaaccatgct ggacgtaaca acaacggccg catcactgta cgtcatcaag gtggcggaca
     8761 caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa gataacgttg ttgcaactgt
     8821 tcaaacgatc gaatatgatc caaaccgttc agctaacatc gcgttagtac actatgaaga
     8881 tggagtgaaa gcttacatct tagcacctaa aggactagaa gtgggtatga aacttgtttc
     8941 aggtccagaa gcagatatca aagtcggaaa tgctcttcca ttagaaaaca ttccagttgg
     9001 tactgttatc cacaacattg aaatgaaacc tggtaaaggt ggacaattga tccgttctgc
     9061 tggtacaagt gctcaagtac ttggtaaaga aggcaaatac gtattgatcc gcttgaactc
     9121 tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca atcggttctg taggtaacga
     9181 acaacacgaa ttaatcaaca tcggtaaagc tggacgctct cgttggatgc gtaaacgccc
     9241 aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca cacggtggtg gtgaaggtaa
     9301 agctcctatc ggacgtaaag caccagtttc tccttggggt caaccagctc ttggttacaa
     9361 aactcgtaac aaaaaagcta aatcagacaa acttattgtt cgtcgtcgca aaaataaata
     9421 attactttga ccatgtgtcg cacattttga gaggaggttc accatgggtc gtagtttaaa
     9481 gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt gaagctcaag caggggttga
     9541 aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca gttttcccaa gttttgttgg
     9601 atataccatc gcagtttatg atggacgtaa acatgtacca gtttacatcc aagaagacat
     9661 ggtaggacac aaattaggtg aatttgcacc tacaagaact tatcgtggac atgctgctga
     9721 cgataagaaa acgaaacgct aatttgaggg gaggataaat ccaaatggca gaacaaatca
     9781 catcagctaa agcaactgca aaaacagttc gcacttcacc tcgaaaagcc cgtttagtaa
     9841 ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc aatcttgaaa ttcacaccaa
     9901 acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc agttgcgaat gcagaaaata
     9961 actttgactt agatgttgaa aacttggtag taagcgaagc ttttgttaac gaaggaccta
    10021 caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc tccaatcaac aaacgtacaa
    10081 gtcatatcac ggtagtcgta tcagaaaaat aaggagggat aatcagtggg tcaaaaagta
    10141 catccaattg gaatgcgtgt aggcatcatc cgcgactggg atgctaaatg gtatgctgaa
    10201 aaagagtatg ctgagttctt acacgaagat ttaagaatcc gtaaatttat ttcaactaaa
    10261 cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg caacaggtcg cgtaaacatt
    10321 tcaattcaca cagctaaacc aggtatggtt attggtaaag gcggttctga agtcgaaagc
    10381 ctaagaaaag aattaaacaa attaactggt aaaagaatcc atatcaatat cgtggaaatc
    10441 aaaaaaccag atctagatgc aaaattagta ggtgaaggaa tcgcacgtca attagaaaac
    10501 cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac gctcaatgcg tgctggcgct
    10561 caagggatca aaactcaagt atctggtcgt ctaaacggag cagatatcgc tcgttcagaa
    10621 ggatactcag aaggaactgt tcctcttcat actttacgtg cggacattga ttacgcatgg
    10681 gaagaagcag atacaacata cggaaaacta ggagttaaag tgtggatcta tcgtggagaa
    10741 attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat aatcatgtta gtacctaaac
    10801 gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg ggaagctaaa ggcggaaaag
    10861 aagtagcatt cggtgaatac ggtttgcaag ctgttgattc acattggatc acaaaccgcc
    10921 aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa acgtggtggg aaagtatgga
    10981 ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat tggggtacgt atgggttctg
    11041 gtaaaggggc acctgaagga tgggttgcac cagtaaaacg tggtaaaatc atgtttgaaa
    11101 tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg tctagcttct cacaaattac
    11161 caatgaaaac taagatcgta aaacgtgagg aaatgggtgg tgaatcgaat gaaggttaaa
    11221 gaaatcagag aattaaccac tgccgaaatg cttgatcaag aaaaacaatt aaaagaagaa
    11281 ttgtttaatc ttagattcca attagcaaca ggtcaattag aaaacactgc acgtattaaa
    11341 gaagtacgta aatcgattgc acgcatcaaa actgtgttgc gtgaacaagc taagtaatta
    11401 tggaaggagg ccattaagcg tatgactgaa ggaagaaatc aacgcaaagt ttatcaaggt
    11461 cgcgtggttt cagacaaaat ggataaaaca attaccgttg ctgtcgaaac aaagaaaaac
    11521 caccctatct atggcaaacg catgaactat tctaagaaat acaaagtgca tgatgaaaac
    11581 aacacagcaa aagttggcga catcgtgaga attatggaaa ctcgtccatt atcagctaca
    11641 aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta ttatctaata acacgagatt
    11701 ttcctatata gattgaatgc aaaatctgaa aggaggatac acaacgtgat ccaagcagaa
    11761 agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa ttttgacgat caaagtcctt
    11821 ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga tcgttgctac ggttaaacaa
    11881 gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta aagccgttat cgttcgaaca
    11941 aaatcaggag ctcgtcgtac agacggttca tatattaaat ttgatgaaaa tgctgcagtg
    12001 attattcgtg atgataaaag ccctcgtgga acacgtatct ttgggccagt tgcacgtgaa
    12061 ctacgtgaaa acaacttcat gaagatcgtt tcactagcac cagaagtttt ataatcttga
    12121 gacacttatc aaaggaggtg cgaaacagtt atgtttgtta aaaaaggcga taaagttaaa
    12181 gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac tagcagcgtt cccgaaaaaa
    12241 gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac accaaaaacc ttctcaagct
    12301 gctcctcaag gtggaattgt tgagatggaa gcgccaattc atgtttctaa cgtgatggtg
    12361 gttgactcta ctggtgtagc tggccgagtt ggctacaaag aagtagacgg caaaaaagtc
    12421 cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa agtttaagga aggagggtct
    12481 tactgaatga accgcctaaa agaaaaatat cttaaagaag taactccatc attgatggaa
    12541 aaatttaact atagctctgt tatgcaaaca cctaaagttg aaaaaatcgt catcaacatg
    12601 ggtgttggtg atgcggtatc aaacgctaaa aacttagata aagcagttga agaattaact
    12661 ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa aatcaatcgc tggattccgt
    12721 ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc gcggagaaag aatgtacgaa
    12781 ttcttagaca aattggtttc tgtttcacta ccacgtgtac gtgacttcca tggtgtaagt
    12841 aaaaaagcct ttgacggtcg tggaaactat actttaggta ttaaagaaca attgatcttc
    12901 ccagaagtag attatgattt agtagataaa gtacgtggta tggacatcgt tattgtaaca
    12961 acagcaaact ctgatgaaga atctcgtgag ttgttgacac aattaggcat gccattccaa
    13021 aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa tgattgctaa aaacaaacgt
    13081 cctgcaaaat tttcaacaca agcttatact cgttgcgaac gttgcggacg tccacattca
    13141 gtttatcgta agttccatct ttgccgtatt tgcttccgcg aacttgccta taaaggtcaa
    13201 attcccggcg tgaagaaagc tagctggtaa aaagtaaatt cgcataagaa ggaggtaaaa
    13261 gatcaatggt catgacagat ccaattgcag attttctaac tcgtatccgt aacgcaaaca
    13321 tggttaaaca tgagtcttta gaagtacctg catcaaaaat taaacgtgac atcgctgaaa
    13381 tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat cgaagatgac aaacaaggcg
    13441 tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg tgttatcaca aacttaaaac
    13501 gtatctctaa accaggtttg cgtgcttatg tcaaagctga cgaagtacca aaagtattga
    13561 acggtttagg tatcgcaatc atttcgactt ctgaaggtgt tgtcactgat aaagaagctc
    13621 gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg gtaatcaagt agaatctaca
    13681 aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg ttgttatccc tgaaggagta
    13741 acagttactc aagacggaaa caacgttaca gttaaaggac caaaaggtga attgactcgt
    13801 actttttcag cagatatcaa aatgaatatc gaaggaaacg aagtaacttt cactcgtcca
    13861 aacgacagta aagaaatgaa aacaattcat ggaacaactc gtgcgaactt caacaacatg
    13921 gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac taatcggggt tgggtaccgt
    13981 gcacaattac aaggatcaaa acttgtattg aacgttggtt actcacatcc agtagaaatc
    14041 acaccaccag ctggagtaac tgttgaagta ccagctaaca cacaagtaat tgttaaaggt
    14101 gcgaacaaag aagaagttgg cgaattagct gcaaacattc gtggaactcg tcctccagaa
    14161 ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg tacgccgtaa agaaggtaaa
    14221 actggtaaat aatcgtttag aaaaattgca tagaagccag cagcaggaaa aaggcatttg
    14281 gctgaagttt accttcaaaa aaatacattt ttacttgtgc cgcagcttct atttttcgaa
    14341 acgtatttac tgcttgaaat agtagtacaa taaaaataaa gaggtgacaa ttgtgattac
    14401 aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt cgtgtacgta acaatatctc
    14461 tggtactgct gagcgcccac gcttgaacgt ttttcgttct aacaaaaaca tctacgctca
    14521 aattattgat gacgtagcgg gtgtaacgct agcaagtgcc tctaccttgg ataaagaaat
    14581 ttcaggtgga acaaaaacag aagcagcaca agctgtcggt aaactagttg ctgaacgtgc
    14641 agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt ggataccttt accatggccg
    14701 tgtgcaagct ttagctgaag ctgctcgcga aaatggacta gaattttaga agaaggagga
    14761 ataccattca tggtttatat cgatccaaaa catttggaat tagaagaccg cgttgttgca
    14821 attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc tacgttttgc tgctttagtt
    14881 gttgtcggtg acaaaaacgg acacgtaggg tttggtacag gtaaagctca agaagttcct
    14941 gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact tagttgaagt acctatggtt
    15001 ggttcaacaa tcccacacga agtaatcggt gcttttagtg gtggtagaat cctaatgaaa
    15061 cctgctgtag aaggttctgg ggttgctgct ggtggaccag ttcgtgccgt attagaatta
    15121 gctggggtag cagatatcac ttctaaatct ttaggctcaa atacaccaat caacgttgtt
    15181 cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag aagaagttgc agaacttcgc
    15241 ggcaaatcag ttgaagaatt aatcggataa ggaggacaac aataatggct gaattaaaag
    15301 ttactttaaa acgcagtatt atcggacgtc ctcaaaacca acgcgataca gttaaagcgt
    15361 taggtcttac aaagattaac agttcagtgg ttaagccttc taatgaagca attaaaggca
    15421 tgatcaatac tgtttctcac ctagtggacg ttgaagaaat ttaatttaga aaaatcataa
    15481 ctagaacatt aaggaggtgc caaacctatg aaacttcatg aattaaaacc tgctgaaggt
    15541 tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat ctggtaacgg aaaaactgcc
    15601 ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg gtgtgcgttt aggatttgaa
    15661 gggggacaaa caccattgtt ccgtcgttta ccaaaacgag gattcacaaa cgtgaaccgt
    15721 aaagactatg cagtcattaa tcttgatgta ttgaaccgct ttgaagacgg agctgaagta
    15781 actcctgttg ctttagttga agctggaatc gtgaaaaacg aaaaagctgg aatcaaagta
    15841 ttagctaatg gggaactaaa caagaaatta actgtgaaag cagcaaaatt ctctaaagca
    15901 gctcaagaag caattgaagc ggctggtgga tcaatcgagg tgatctaatg tttagactat
    15961 taaaagatgc ttttaaagta aaagacatta gatcgaaaat cctatttact gtatttgtgt
    16021 tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg cgtaaatgct gcttcattgc
    16081 aaaacctttc ttctttgcca tttttcaaca tgttgaactt ggtaagtggt agtgcgatgc
    16141 aaaacttttc cattttctca atgggggttt caccatacat tactgcttct atcgtcattc
    16201 agcttcttca aatggacatc gtgcctcgat ttgttgaatg gtcgaaacaa ggggaagttg
    16261 gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt agtattggca tttatccaat
    16321 ctgtcggtat cactgctgga ttcaatcaat acgctcaatt aggtttcgtg aataatccta
    16381 acgtagcaac ttacgtaacg atcggtttga ttctaacagc cggtacgatg tttgttactt
    16441 ggctaggaga acagatcaca gaaaaaggta tcggaaatgg cgtatcaatg atcatttttg
    16501 caggtatcat ttctagattg cctgaaagta tcagagaaat ttatgaagat tactttataa
    16561 atattgatcc ttcagacata tggaaatctg ctatttttgt ggccatttta ctagtagcgg
    16621 tcttgttgat cgtgacattc gtaacatttt tccaacaagc tgaacgcaag atcccgatcc
    16681 aatatacgaa acgcgtagct ggcgcgccta caagcagcta tctgccgttg aaagtaaatg
    16741 cagctggagt tattccagtt atctttgcta gttcgtttat tgctacgcca aatgctgttt
    16801 tacaagcatt gagcggtaga ttcggttctg aaaactggta caaagtgatg atggagatct
    16861 ttaactataa tacagcacct ggtgctacga tttatacgat tctgatcgtt gccttcactt
    16921 tcttctacgc ctttgttcaa gtaaaccctg agaaattagc ggaaaacttg caaaagcaag
    16981 gaagttatat accaagcgta cgccctggta aggggactga agagtatgtc tcaagattac
    17041 tgatgcgttt aagtacagtt ggtgcagtct tccttggttt agtggcattg cttccaatca
    17101 ttgctcaaat ggtttgggat cttccacaat caatcggatt aggcggtaca agcttactga
    17161 tcgttatcgg tgtagcttta gaaactgcga aacagttaga aggcttaatg ctgaaacgta
    17221 aatatgttgg attcattgag taagaaagcg tgttgaggtt tcccctcaac acacgttctc
    17281 acacatttat taggagggaa aacaatgaac ctcattttaa tggggctgcc aggcgcaggt
    17341 aaaggaacac aagctgaaaa aatcattgat gcttatggta ttccgcacat ctcaacagga
    17401 gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg gcctggaagc aaagtcttat
    17461 atggataaag gtgcattagt tcctgatgaa gtaacaaatg gaatcgtgaa agagcgacta
    17521 gctgaaccag atacagagaa aggcttctta ttagatggct ttccacgtac gctagatcaa
    17581 gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa aaattgatgc tgtcatcgat
    17641 atccatgttg gtgaagaaat cttggtcgaa cgtctagccg gccgatttat ctgcagcaat
    17701 tgtggagcaa cttaccataa aattttcaac cctacaaaag ttgaagacac atgtgatcgt
    17761 tgcggtgggc atgaattcta tcaaagagaa gatgataaac ctgagacggt taaaaatcgt
    17821 ctggctatca acatcaaaaa tagtgaacca atcttggctt attataaaga acaaggtttg
    17881 ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat ttgcagatgt acaaaaaatc
    17941 attgaaaaat agcacgattg cccgtagccg acttgcatta tgcagttact cttgtcaagt
    18001 tcaataaatt atgatagaat atgtcagtcc gacttctaga gaatcctcta gatgatccat
    18061 tttgagaatt ttgaggtggg aactgcagtt tccgccattt tatcgtgtga aaatgcgaaa
    18121 caagtacaag gaggtactgt gcgtggcaaa agaagatatg attgaagtcg aaggtacagt
    18181 cgtcgaaact ttgccgaatg caatgtttaa agttgaacta gaaaacggac accaagttct
    18241 tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt atcttacctg gtgacaaagt
    18301 aacggttgaa ttgtctccat acgatcttaa ccgtggccgt attacttatc gctttaaata
    18361 attgtactcc gtaaaactac aggaggtatt atcatgaaag taagaccatc agtaaaacca
    18421 atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg ttatggtgat ttgcccagca
    18481 aatccaaaac ataaacaacg tcaaggataa ttggaggtgc tataaatatg gctcgtattg
    18541 caggagtaga tattcctcgt gataaacgtg tagtagtttc acttacttat atctatggta
    18601 ttggtaacac tactgctaaa aagatcttag ctgatgctgg tgtatctgaa gatgttcgcg
    18661 ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc agaagtagat aaattaaaag
    18721 ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa acgtttgatg gaaatcggtt
    18781 cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg tggacaaaac acgaaaaaca
    18841 atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg caagaaaaaa taattcttaa
    18901 gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa tcgtaaacgc cgtgtgaaga
    18961 agaatataga agcaggtatt gcacatatcc attctacatt caataacact atcatcatga
    19021 tcactgatac tcacggaaat gcattagcgt ggtcatcagc tggttcatta ggtttcaaag
    19081 gaagcaaaaa atcaactcct tttgccgctc aaatggcagc agaaactgct acaaaagcag
    19141 caatggaaca cggattaaag actgttgaag taacagttaa aggacctggt tctggacgtg
    19201 aagcagcaat tcgttcatta caagctacag gtttagaagt gactgcaatt cgtgacgtga
    19261 ctccagttcc tcataatggt gcccgccctc caaaacgccg tcgtgtttaa tgagtgccgc
    19321 tcattttgag ttttatcagt aacgtcattg aacaagacac ttttcgtttt gaaaggggta
    19381 aagataagaa tgattgagtt cgaaaaacca agaatcgcaa aaattgatga agaaaaagat
    19441 tatggcaagt tcatcgttga acctcttgaa agagggtatg ggactacttt agggaattcc
    19501 ctacgtcgta ttttattatc ttctttgcca ggtgctgcca tcactaatat tcaaatcgat
    19561 ggtgtactgc atgaattctc taccatcaaa ggtgttagag aagacgtcac tcaaatcatc
    19621 ttgaacatta aaggcttagc gctgaaaatg tatggccaag aagaaaaaac ccttgaaatc
    19681 gatattaccg gacctgctac agtaactgct ggcgatatta tcgttgatag tgatgtagaa
    19741 attcttaaca aagatatgta catctgtagt gtctctgaag gagctacgtt ccatgctcgc
    19801 ttaacagtga aacctggacg tggttatgtt caagcagatg aaaataaaaa agaagatatg
    19861 ccaattggcg ttcttccagt cgattcaatc tacacacctg ttcgtcgtgt gaactatcaa
    19921 gttgagaaca cacgtgttgg acaccgtgaa gattttgata aattgacgat ggagatttgg
    19981 acagatggtt caattgaacc gttggaagca atgagtttgt ctgcgaaaat catgactgaa
    20041 catttggata tttttgtgaa cttaactgat gaagcaaaaa atgctgaaat catggtcgaa
    20101 aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga tcgaagagtt agacttgtct
    20161 gttcgctctt acaattgttt gaaacgtgca ggaatcaata ctgtacaaga acttacaaat
    20221 aaatctgagc ctgaaatgat caaagtccgt aacttaggac gtaaatcatt agaagaagtt
    20281 aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag aagattaaaa ccttttacga
    20341 aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca catctagcca acgtaaagcg
    20401 atgttgcgtg atttaacaac tgatttatta atcaacgaac gtattgtgac tactgaagct
    20461 cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca ctttaggtaa acgcggagat
    20521 ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg aagttgccag cgtgcgtgaa
    20581 gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac tgttcaacga tattgcacct
    20641 cgttacgcag aacgtcaagg cggatacaca cgcattttga agacagaacc aagacgcggt
    20701 gatggtgcac caatggttgt cttagaactt gtctaaaatc acactaataa catcacttta
    20761 tagatgattc ttttagagtg ttatgatgtt ggaagatatt tttccgagtc tagctcagcg
    20821 ctgctcctca agcgaatatc gcttggggag taagcactca tcataaagtg tttttttata
    20881 ccttcaatta cctgtcatga cagatatgtt tgaaggcata aaaaaacatt ttaagcgaga
    20941 aataattgaa aattttgtct ttttttgata gtgttagtag gaactttagg atgaagaagg
    21001 agtaatcatg gagccaatta ttgaattaga aaagataaat tataaatacc agccagatga
    21061 tcttcgcccc gcattgaaag atgtctcctt tacaattgat aaaggtgagt ggatcgctat
    21121 tattggacac aatggttcag gaaaatcgac acttgccaaa acgatcaatg gattgcttct
    21181 gccagaatca gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa atatttggac
    21241 gatccgacag atggttggaa tgatttttca aaatccagac aatcagtttg ttggttcgac
    21301 agtagaagat gacgttgctt ttggtcttga aaatcaaggg attcccagag aagaaatgct
    21361 agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct gagtttgctt cacgcgagcc
    21421 tgctcgttta tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg ttgccttacg
    21481 acctgatatc attattttag atgaagcaac cagtatgcta gatccagaag gtcgtgaaga
    21541 agtgatctct acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta tatctatcac
    21601 tcatgatatt gatgaagcag ctaatgccaa tcggatacta gtgatgagac aaggcgaact
    21661 tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt cctgaactga tcgacttagg
    21721 tctagatcta ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg gggttgatgt
    21781 gcccagtgaa tatatgacag aagaaaggat ggtggattgg ttatggacat ccgttttgaa
    21841 caagtagact tcacttatca gccgaataca ccatttgaac aaagagcctt atttgacatc
    21901 aatatgacga tcaaagaaaa cagttatact gcattagtag ggcataccgg aagcgggaaa
    21961 tcaaccttac ttcagcattt gaatgcgctt gttaagccaa caagcggtac agtccatatt
    22021 ggagaacgag atattcagcc ggatacggat aataagaatt taaagcccat ccgaaaaaaa
    22081 gttggcatcg tctttcagtt tccagaagca cagctattcg aagaaacggt agcaaaagac
    22141 attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt cctagcaaaa
    22201 gaaacattag aacaagttgg gctggatgaa agctatttgg aacgttcgcc atttgaactt
    22261 tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag gccggaagta
    22321 cttgtattgg atgaaccaac ggcaggactt gatccccagg gcagaaaaga aatgatggag
    22381 atgttttggc gtcttcataa ggaacagcag atcactattg tattagttac gcatttaatg
    22441 gatgatgtag ccaattttgc agattatgtc tatgtgttgg aaaagggacg aatcgttaat
    22501 agcggggagc cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa acaactaggc
    22561 gtacctactg ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa ctttgctacc
    22621 ttgcctttga cagctgaaga attagcagac gctatcgtcc attatgctgg aggaaatgcc
    22681 catgatgaat aaattgatat ttggtcgtta tatgcctgga gattccttga tccatcgatt
    22741 ggatccccga gctaaattat tagccagttt ttattttatc ggaatcattt ttttagcaaa
    22801 taattgggaa acgtaccttt tgttggcatt tttcacatta ctttctgtct tcttgtcaaa
    22861 agtagatttg aaatttttta tccgaggcat tcgcccattg atctggctaa tcctcttcac
    22921 tgtagctttg caggtcttgt ttacaagtgg cggagaaact tattggagtt ggggtgtttt
    22981 ccatatcact gaatttggtg tgatgaacgg cctctttatt ttctgtcgat tcgtgttgat
    23041 catttttatg tcgactctac tcacgcttac tacaccgcct ttggaattat cggatgcgat
    23101 tgagtacttg cttcgtccat tgaaagccgt acggttccca gtccacgaaa tttcattgat
    23161 gctgtcgatt gctcttcgtt ttgtgcctac attgatggac gaaacagaaa agatcatgaa
    23221 cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga aaatgaaagc
    23281 agtcattcct ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg aagatcttgc
    23341 gactgctatg gaagcaagag gctacaaagg tggggaagga agaacgaaat atcgtgtatt
    23401 gcattggcat aatagagaca cgatggttat cattgctttt gtattattaa ctgtgctact
    23461 gatcttttta agaggataaa cgttcagtgg gccgctatct attcttttct tgctgatctg
    23521 tttgcaagaa aggatgcaag cagtgtatga caagaaatat tagggtctgt ggcagactaa
    23581 ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga aaaaatttta tagaaaaatc
    23641 attattccag ataacctttt aggaacaaag gagagaaaaa catggtacgt tacaaagcaa
    23701 tcattgcgta cgacggaaca aattttaatg gattccaaaa acagccaaat gggcgtactg
    23761 ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa tggaaaagaa atcacggtct
    23821 ttggttcggg aaggacagat gccggagtac atgcgatcgg gcaagtaatc cattttgatt
    23881 atccagagga gcgtccgtta gaacgaatgc gtttcgcgct agatacccag tcaccagagg
    23941 atattgctgt aagacaagta gaaattgttt cagaagactt ccacgcaaga tatttggtca
    24001 aagaaaagac gtatcaattt cgagtggata tagggaaacc acgcagtcct tttcgacgcc
    24061 attatgcaag ttattttcct tacccacttg atttggaaaa aattcagcgg gcgcttcctg
    24121 atttattagg gactcacgat ttcacctctt tttgtgcatc aggaagctcg atagaggata
    24181 aaatacgtac gatctacgag gctaagatgg aggtaaatga aacaaaagac gaattgttgt
    24241 ttacatttag gggaaatggc ttcttgtata agatgatacg gatcttagta ggaacgcttt
    24301 tgaaaattgg caatggccga ttgcctgaag atagtatccc agatattatt gcgaaaaaag
    24361 atcgtaatgc agcaggacct acagcacacc ctgaaggact ttatttgtat gaagtgaaat
    24421 atgaataaga ggttatgata gaagtggtca gcttcaagaa ataagaaggt atttacgaaa
    24481 attacttctc aaaattttgc aaaatttcgg cttattttcg aagaagctac ttctggaaca
    24541 ccgtttaaga aagaggtcgt gagcctgtca tttaactctg agtcacaaag agcaatttca
    24601 aaaaacagct tctcatcttt gatgaatatc gtgacttgtg gacgaggcgt tgataagtga
    24661 atcatctttt tgttacctat tttttctgta accaaaaaac gatggaacgt tgttttgatt
    24721 gcccttggtg cggacagtct gaacaggttc catcgtttct ttattttcta cgtcttcttc
    24781 tgtgtgtgtt tcgtggttgt tcttctattg ctggatcaat cgtgccataa aaggggcgat
    24841 tgcggtcaag gtaccagaca taaagaataa acaatgcaat acataggaca aaaaggattg
    24901 cacctatcgt aaatccttca ggcaagtaac tgaaagtgac agtatgttta cctgctggaa
    24961 cttttatcat caagaatgcg tctttgaaat tgctgacctt gactggtttc ccatcaattt
    25021 tagctttcca tcctttgtca taaggaatag ttgtcaccaa catggaatct tcagcggtat
    25081 ttacagtccc agaagctttt ctgccagtgg cttccatttc gacacctttt tctttgatct
    25141 gattcattgc ggcttgatag gctacaacat ctagaccaac gactttaggc tccatgaaac
    25201 ttatttcttt cgtcccataa aaacttgcag taaatgtcac tgttgtctct tgaggataat
    25261 agccaagatc atagtattga ccagaaatgt tgatttgcgt ttttcgcgat gtaccattga
    25321 cagtgatcgt cgcactagaa ctttctaatt gcgcaaaatt ggttgggaac aaacttaagt
    25381 aagcttgtgt atttgcaggc acatggactt cccaagtgag ctcttttggc tggttatctg
    25441 ctgtttctga gtaagtgacg ccagtcgttg tctttgtgat cgtcgtattc aactgattct
    25501 tcaaagtagg tgtatagaat gtaaaatact gttcatttag accagacaat gtattgatca
    25561 agtttgtctg actggtcaaa ttatctgtca ctggctgctc aacatcatta acggacaacg
    25621 gtgcagtaaa tgctagtggc aatgcattga cattctgata cagtgtatag tcgccgcttt
    25681 gaccagcttg gcggaagcca tatttactga acgtgctttc gtttttagaa atgttatatt
    25741 tgattcctgt aaacccatcc atcagcaaag tattattgtt ataacggata ttcaaatttg
    25801 tccctcttga acggaaacct aatttatcca ggtaagagga agaattacga tttcttatag
    25861 aagaaaacaa actgatacca ctgtaaccat agttgatcga atcgtttgca gatactggat
    25921 ccagattttc caagcggtaa aacgagtcgg aatctttttt tgtttcattt acaagagctt
    25981 tcaagtcagg ataaggatca gtatataaac tgcgggaagc ataattccaa tcatcaagaa
    26041 tgccatggat catactgttc gtattgacca gtgcttcagc agacatgatc aataataaca
    26101 atatactcag atgattgaat gaaagttttt tatgctgata aaatcccaca gcaaaagcat
    26161 aaatccctaa gaaaaccact gtcaatacaa aggaagtcat cgtcacatag gtgtagcttc
    26221 cttcaggttt gatgccccat gccaccgcaa agagagcagc tacgaccaaa atgattccta
    26281 acaacagccc accatcgtct tgttctatct tttcccatcc ttttcctgca agcaggagca
    26341 ctaagaaaga gaacagatag gcataacgga ataaaaacat attcggtgca tgcataccat
    26401 gccagaacaa attcaatgga acaagataaa aacttgcgat aagtatggcg aaaagactgc
    26461 cgtacaaaaa tttatttttc aacggtactt tccgactggc aaaataaaac agacagagga
    26521 ctaatggtag cagacctgcg taaacgaacg gaatcgatcc gtattttgtc gtgtcataaa
    26581 caccaatcat atttttcatg attagatcca gataagaagt cgcttctgtt ttcaacgtag
    26641 tgatctgact cagtgcctcg ccatttgacc gaagatccag cactgctggc aatacaatga
    26701 tcattgaagc gccgcctgcc agtaaagaag tgataccata aggaacaatc gtttttttat
    26761 attctgacca gtttgtcaat agacgggcga taaaatataa gaaagaaaat acgccaatca
    26821 taaaacccat ataaaaactg gataaaaaca agagtaaata actgataaat aaaagtttcg
    26881 gccgtttaag atccataaca cgatgaatcc ccaaaatcac taatggaaga taaacaaatg
    26941 catccagcca catgatgatc tccgaatgtg cagtgataaa tgagatcaat gcgtaactga
    27001 cgctaagtgc aacatgcccc cagcgaggaa tcttgaacgt acgtgcaaga aaccaaaaag
    27061 ccaatccggc acagccgacc ttaagcaaag tcagaacata cagtgcatct ggcatcatct
    27121 ggtttggaaa aaagaacacc agtggcgtga agatcccgcc tagatagtaa gaaattaaag
    27181 ataaataatt caatcctaga gaagcattcc atgtataaaa aatactttgc tttccaagaa
    27241 gaacatttcg gaagctggca tgaaaattag aaaactgtga aaaggcatca cttgccaaaa
    27301 tacttctgct gcttccagga tagattccga tacttagata aacaagtgct aaaatcaaaa
    27361 acggaatcag gaaactagct gctgcgtagc gtccgttttc tctaaagaat gactttaaat
    27421 tttttttcat atttattttc caccaaattt ctatgaagtt gtattcagtt tatcatatcc
    27481 ttgtttttct ctgcttaaaa agagtctagt tttcacaaaa aaataaaatt tagaataaat
    27541 aacttcataa tttgttcgta taacaaaaat aaacgcaaaa aaagggtagg aatcaaaagt
    27601 cccaccctta aaaattttta ttaaataaaa aaccgttcac tatctgaacg gtccaagtat
    27661 cattcaatta aacgcgttca acttttcctg atttcaaagc acgagttgac acccaaactt
    27721 ttttaggttt tccgtcgacc ataacgcgaa ctttttgtaa gttaggttta acagtacgtt
    27781 ttgttgagtt catagcgtgt gaacggttgt ttccactttt tgtcttgcga cctgtaaagt
    27841 aacaaacttt tgccatggta atttcctcct ttgccttgat ttggagcaat agaataattc
    27901 agctcatacc agactaacat atcatataga atgggaattt gcaagtacat tttcatagat
    27961 acaattgaca attgagaaga ataattgtaa attaaacaag gttatacctg aaattttgca
    28021 aggtttttga gtactttggg taactgcctt ttatttcaag ttgcgctatg ataaaatatg
    28081 atagaagaaa aatgggagtt ttttacccgt taaggaggct cattcatggc tgtaaaaatt
    28141 caaacgtcag ctggaaccat tgaaattacc aatgatgtaa ttgcaacagt tgttggtggc
    28201 gccgcgacag atgtgttcgg tgttgtagga atggccagca aaaatcaaat taaagataat
    28261 attactgaga ttttgcgtaa agaaaattac tcaagaggag tagttgtccg ccaagaagat
    28321 aatggtattg ctgtagatgt ttatacgatt atcagctatg gtacgaaaat ctctgaagtt
    28381 tcccgcaacg tccaagaaaa agtgaaatac aatctagagg cattattagg cgtaactgcc
    28441 aattcagtta atgtctttgt acaaggtgtt cgtgtactgc cagactaggc aggagactgg
    28501 ctggtaactc agcagtcgat ctgaaggagg attatctagg tgagtgtaac agaaataagt
    28561 gcaagccagt tccaggaaat ggtccaggca ggtgcgaatc gtctgcaagt aaatgcagaa
    28621 tacgtcaatt cattaaatgt attccctgtg ccagatggcg atacgggaac aaatatgaat
    28681 ctatctatga ctagtggagc taaggcagta gtagattcaa cctcagaaaa agtaggagaa
    28741 ttagcagcaa tgctttcaaa aggattgctg atgggtgcgc gcggaaattc cggcgttatt
    28801 ttgtctcaat tgtttcgtgg attttctaag caaattccag atgttgtctc attaaatgct
    28861 tctgatcttg ctgcagcctt cactcatgga gttgaaactg cgtataaagc agtaatgaaa
    28921 ccagtagaag gaacgatttt gacagtagct agagaagcag cgaaagctgg agaaaagaaa
    28981 gcaaaaagta ctgatgatgt cgttgaagta atgacagctg ttgtaaaagg agcaaagcgt
    29041 gctttagcta aaactccaga cttgcttcca gtcctaaaag aagtcggtgt cgtagacagt
    29101 ggagggcaag gactgctgtt tgtttatgag ggctttttaa gtgcattgaa tggagaattc
    29161 caagcggatg aaacatatga accttctcca gcagaaatgg atgatatggt caatgcagaa
    29221 catcatcgca gtgtccaagg tcaaatggcg acagaagata tccattttgg ttactgtaca
    29281 gaaatcatgg taaaaatcgg agaaggacct acagtcgata gtacattcga ttacgaagaa
    29341 ttccgtaact atttagatgg tataggagat tctttacttg tggtcaatga tgacgagatc
    29401 atcaaagtcc acgtgcatac agaaaaccca ggagaagtct tgaattatgg acaaaaattc
    29461 ggttcattag tcaaaatcaa agtcgacaac atgcgcttgc agcatgagac gattttggaa
    29521 cacgatgaag aagtgaacga gtttgcgaaa aaagaaatac cacgtatccc gtatggtatc
    29581 gtagcgatcg ctgctggtaa aggcgtccaa gagctgtttg aaagtttagg cgctaattat
    29641 gttatcagtg gtggacaaac aatgaatcca agtacggaag acattgttaa agcgattcaa
    29701 gaagtaaacg cagataaagt gattgtcttg ccaaacaata agaacatctt catggcagct
    29761 gatcaagcag cagaagtagc agatatccca gtagcagttg ttccttcaag aacgatttcc
    29821 caaggattga cagctatgct ttcattcaat gatcaagctt ctttagaaga aaacaaagaa
    29881 acgatgacag aaatgttgtc tagcgttgtg agcggtcaag tcactcacgc gatcagagat
    29941 acctcaattg acggtgtaga gatcaaagaa ggagactttt tgggcatgat cgatggaaaa
    30001 atcctgatct ctaacccaga tatcacagca acagcgttaa gtacactaga acaaatgatc
    30061 aatgaggaca cagagatcgt aacgatcttg gcaggtgatg aaggaacagt tgaacaagca
    30121 aatgaatttg ctgagaaact aacagaaaaa catcctgatt tggaaatcga gatccaccaa
    30181 ggagatcagc ctgtctatcc ttatctattt tctgctgaat aatttctgtg tcagaatatc
    30241 tttctaacac agtattcatt cataaaaggc cttcggttag tgatcaggta tgatcgctga
    30301 ccaagtcctt tttttgttaa aggagggagg aaaaatttat gcgttcttta gatgatccaa
    30361 tcactatgct ggcaggagtc ggagaaaaaa gagcagctag tcttgccagc ttgggcatcc
    30421 aaacgattga agacttactg acttattatc cttttcgcta cgaagatatt caggaaagaa
    30481 atctaaacga gatccaggat caagaaaagg ttactttgaa aggaatcgta gtctcgcctc
    30541 ctgtgatgaa tcgatttggt tataaaaaaa gccgattgca atttcgtatg atgcaagaac
    30601 atgatgtgtt caacgtttct ttttttaatc aaccttatct aaaagaaaaa gtcattttat
    30661 cggaggaaat cgccgtatac ggtaaatggg atgcaaagcg taaagccttg aacgggatga
    30721 agattttagg ttctcaaaca atggatgact tttcgccgat atatcacgtc aacaaatcaa
    30781 ttcgtcaaac gacattagtt gatctaatca aaaaagggtt tcaagaatac ggcacattga
    30841 ttgaagaaaa tctgccaaac aaccttgtgg agaaatatcg actgcttgac cgagcttctg
    30901 ctgtaagaag tatgcatttt ccaaaagacc acgaagaaaa ccatcaagcc aaacgacgtg
    30961 tcgtttttga agagttcttt ttatttcaaa tgcgtcttca aggattgaaa aaagcagaaa
    31021 aagcagaaac gaatggtttg gagatccttt atgatgtcca aaaattgaaa caatttactc
    31081 aaaagctacc ttttgaactg acaaatgcac aaaaacgtgt aacgaatgaa atctgtcggg
    31141 atcttcgcag tccgcagcat atgcagcgac ttttgcaggg agatgtaggt agcggaaaaa
    31201 cagtggttgc cgcgattgcg ttatacgcta ccgttacagc aggtttccaa ggtgcgttga
    31261 tggtgcctac agaaatcttg gcacaacaac atatggaaag cttgcaacag ctgtttgatc
    31321 ccaatgaagt aaccatcgca ttgattacgg ggtcaacgaa agcaaaagaa cgacgtgagc
    31381 tccttgaaaa actagagcaa ggggaaatta acgtcgtgat cggcacacat gcattgatcc
    31441 aagatggagt cgaatttcag aatttaggtc tagttattac ggatgagcag caccgttttg
    31501 gcgtgaacca gcggaaagta cttcgtgaaa aaggctggcg accagatgtc ttatttatga
    31561 cagcgactcc gattcctaga acattagcta ttacagcata tggtgagatg gatgtatcta
    31621 tcattgatga acttccagct ggacgtatcc cgatcgaaac acgctgggta agaacacctc
    31681 aattggacag tgtactggat tggacgaaaa aagaactggc aagaggacat caaatgtatg
    31741 tcatctgtcc gctgattgaa gaatcagaag cattagatgt aaaaaatgca gtggaaatct
    31801 atgaaaaact tcgtgaacta tttgcaccaa aatatgaagt tggcttgtta catggtaaaa
    31861 tgaagaatca ggaaaaagaa gtaatcatgg aagccttcaa agacaaccag atgcagatat
    31921 tagtttcgac tactgtaatt gaagtagggg taaacgtgcc gaacgcgacc gtcatgctga
    31981 tcatggatgc ggatcgattt ggattggcac agctgcacca acttcgtgga cgtgttggac
    32041 gcggaagtga tgcttcctac tgtatcttag ttgctaatcc taaaaatgaa ctaggtgtgg
    32101 aacggatgaa aatcatgaca gagaccacca atggttttgt actaagcgaa aaagatctgg
    32161 aattacgtgg tcctggtgaa gtatttggct tccgtcaatc tggtctgcct caatttgcga
    32221 ttgctgactt ggtgacggat ggaaatgttt tggaggtagc cagagaagag gctgcagccc
    32281 tttggcaaat aaaggattgg cagatgcttc ctgagtatca gcctttagcg caaagcctcc
    32341 aaatagaaaa tcaggataat cgttatttcg attagcagac tgtgctatac tttatactga
    32401 tgacggaaaa aggagtggaa acaatgaaaa ttgccgtaga tgcaatgggc ggagatcacg
    32461 cacctcaagc aattgtagaa ggcgtggctt tagcgcagca agattttcca gaagtagaat
    32521 tcttacttta cggaaaagaa gcagaaatca agaaatactt aacaaatgaa aaaaacatca
    32581 cgattatcca tacagacgaa aaaatcaata gtgatgatga gccagtaaaa gcaattcgac
    32641 gaaaaaagac tgcatcaatg gtccttgctg cacaagcagt gaaaaacggt gaagcagatg
    32701 ctgtattttc agcagggaat acaggcgcgc tactagcagc agggttgttc atcgtaggac
    32761 gtatcaaaaa tatcgaacgc cctggcttga tgtctacctt gccagtagta gggaaagaag
    32821 gtgccggttt cgatatgctg gatcttggcg ctaacgcaga aaacaaacca gaacatttgt
    32881 tgcaatacgg tattttaggt tctttttatg cacgtaaagt ccgaggtatc ggacgtcctc
    32941 gtgtcgcact cttaaacaac ggaacagaag caacaaaagg tagtgaagtg acgaaaaaag
    33001 cctatgaact ccttcagaat gaaacaagct tgaattttat tggaaacgta gaagctcgtg
    33061 agctactcaa tggtgtggct gatgtagtgg tcacagatgg atttacaggc aacgctgttt
    33121 tgaaatctat tgaaggaaca gcaatgaata tgatgaatct attaaaatct gctattctaa
    33181 atgaagggat caaaggaaaa atgggcgcta tgcttttaaa agacggctta cgttctttga
    33241 aagcagaaat ggattattcc aaacatggcg gtgccgtttt gtttggcttg aaagcaccag
    33301 tcattaaaac tcatggagca acaggccccg atgctgttcg ctatacgatc cgccaaatcc
    33361 acacaatgct tgagacagat gtagttggac aattagtcga acaatttgaa aaaagcgaag
    33421 aatagtattt ggatatctga agcgatccgt tccattgaaa aaaaggtgga cattcctgaa
    33481 aaatgccagt aaaattagcc atggtgtagg taattgacaa gtgtatgatt cttggaggtg
    33541 aaaaagttga ctcgcgaaga agtatttaac aaagtagcga aaattatttc aaatcatttt
    33601 gagatagata ccgataaagt gacggatgaa ttgaacatca aagatgattt aaaagctgat
    33661 tcaattagta ttatggaatt cgttttagag cttgaagatg aattcggtac ggaaatttca
    33721 gatgaagacg ctgaacaaat tgaaacagtc ggtggcgcag tagattatat cagcagccat
    33781 cttaaataat ttcaaataaa agcaaagaag gataaaaagc tgcatgcagc tttttatcct
    33841 tcttttttta atctttttga cacgtaaaag tcatttttct gataattctg gcgataaaca
    33901 gaaattatgt tgcaaagtaa agcggttttc tatataatta ttaataattc attacacaaa
    33961 gataagaata acctttgtta taaaacgatt tattctgtgt aatgtccttt tttattaaga
    34021 aaaacttcca tttaggaagc agagaggggt attcgattgt ccgataacca attattagaa
    34081 gttcagaacc tacatacagg attccgactc aaagatgact attacgatgc agtagatgac
    34141 gtttcattta ctttagacaa aaacgagatt ttagcaatcg ttggggaatc tgggtgcggg
    34201 aaaagtacct tggctactac gattatggga ttacttgatc ctaataacac aaaaattaca
    34261 ggtgagattt tatataacga tctgaattta atcgacctga acgaaacgct ctataataaa
    34321 atccgcggaa atgatatcgg aatgattttc caagatccgt tgtctgcatt aaatcctctc
    34381 atgagaatcg aagaccagat caaagaagga ctttcctatc acacaaagat gaatgcagaa
    34441 cagcgtcaag ctcgtgcact agaattgcta gaacaagtag ggattcctaa tcctgcgaga
    34501 gttggacgcc aatatcccca tgaactatca ggcggtatga gacaacgtgt catcatcgca
    34561 atcgcaatcg cctgcaaacc tccaatcgtc attgcagatg aaccgacgac agccctagat
    34621 gtaaccatcc aagcacagat tcttgattta ctgaaagacc ttcaggaaga aacaaaaaca
    34681 gggatcatct tgatcactca tgacttagga gtcgtagcag aaatggctga tcgagtggca
    34741 gtgatgtatg gcggacagtt cgttgaagta gcaggtgtaa aagaactgtt tgatcatcct
    34801 aagcatcctt atacacgctc tttactgaac tctataccac aagaaggaaa tcatgatgca
    34861 gaactccatg tgatcgaagg tgtcgttcct tctctgaaaa acatgcctcg taaaggttgt
    34921 cggtttgcac cacgtattcc atggatacca gcaagcgatc atgaagaaga gcctatcctt
    34981 catgaagtcg caccgaacca cttggtacgt tgctcctgct ataagcattt ccactttaga
    35041 gacgaaaaag gagaggtgta gatgacagaa ttaatcacaa tcaaagacct gaaagttcat
    35101 taccctattc gcagtggatt cttcaatcga gtaacggatc atgtatatgc tgtagatggt
    35161 gtggatttta ttatcgaaaa aggaaagaca tatggtttag tgggagagtc tggctctgga
    35221 aaatctacta cgggaaaagc cgttgtggga ttagaaaagg taacagccgg acaaatcatg
    35281 tatgaaggaa aagatgtaac gaagcgaagc aaccgcaaaa aaatgggtta taacaaggat
    35341 gtgcagatga tcttccaaga ttcaatgtcc agtttgaatc ctaaaaagcg agtgttggat
    35401 atcattgctg aacccatccg aaattttgaa cggttaagcg atcaagaaga aaagaaaaag
    35461 gtaaaaggac tgctagatat cgttgggatg ccggaagatg cgctatataa atatcctcat
    35521 gaattttcag gcggacaacg tcaacgtcta ggagtagccc gtgctgttgc taccaatcca
    35581 aaactgatcg tagccgatga acctgtatct gctctggatc tatctgttca agcgcaagta
    35641 ttgaatttca tgaaacgaat ccagcaagaa tacggcctca gctatttatt catttcacat
    35701 gatttaggtg tagtaaaaca catgtgcgac aatatcgcaa ttatgtacaa aggtcgattt
    35761 gtagaaatcg gtacaagaga agacatttat aatgacccgc gacatatcta tacgaaacgt
    35821 ctgctttcag ctattccgca aatcgatgta gaaaataggg aaagccacaa agaaaatcga
    35881 cgaagagtag aacaagagta tatccaaaac caaaaagaat actatgatgc gactggtcga
    35941 gtctacgatt tgcgtacgat cacaactaca cacaaagtgg cgttgaaaga tggaggtgca
    36001 agctaatgtg gaaaacgatt ctgcgacgtg ttttattgat gatcccgcaa gttattattt
    36061 taagtgttct gatcttctta ttagcccaag cgatgccggg ggatccattt accggtttga
    36121 tcaatccaaa tcaggatccg gcagtgatcg aacagatgcg acaagcagct ggcttgaatg
    36181 acccatggta tgtccagtac ttccgttgga tcggaaatgc tttgcaaggc gattttggtc
    36241 aaagtttctt atacaaacgc tctgttgctg acttgatcgg cgaacggatc atcaacactt
    36301 tatatctatc gatcttgaca gttgtcctta cttatttgat tgctgtgcct ctggggatgc
    36361 ttgctggccg gtatcaaaat tcaatcttgg ataagattgt cgtaatctac aacttcttta
    36421 gttttgctgt tcctttattt atcttcggct tgatcatgct atttgttttt ggttatcgtt
    36481 taggctggtt cccaacaagt ggatcacaaa cactaggttt gcaaggtgga tttatcactc
    36541 aatggataga ccgtttgaaa tatattctgt taccatcaat tacccaagca tttttggcga
    36601 ctgccgtcac aatccaatat ctgcgaagtg aagtaatcga ttcgaaatcg ctcgattttg
    36661 tcagaacagc ccgttccaaa ggggtaccga cgaataaagt attcagtcga catattttcc
    36721 gaaatgcagc gttgccgatg gcttctcaaa tggggtatga aatcacttcg ttgattgctg
    36781 gttctgtagt tatcgaaaag atttttggtt atccgggagt cggaaaacta tttattgatt
    36841 ccatcggcca acgggactat accgtgatca catccttagt attgatttta ggaatcgcga
    36901 cgctagtggg gacattactt tcagatatca tcatgagtat cgttgacccg cgtattcgga
    36961 ttcaataaaa ccaatcagaa aggagaatgg atcgatgagt gaaaaaaaca atacagcagt
    37021 tcaagaaagt attcctccta tggggatacg aatgattgct cgagaattta aaaaagataa
    37081 aattgctatt ttttcattag gtttgctagt ggttttatta ttagtgatct ttatcggtgc
    37141 gatgctgacc gatcaagaca aagtgatgta tgttagtatc ttcgacaaat acgctgcacc
    37201 aggaacagtt tcgacacaag gacaaaagtt cattctggga gcagatgaag gcggacgtga
    37261 tgtattagga caattgatca taggtgctag aaactctgtt acgatcggat ttgcgatcac
    37321 gatccttact tcgatcatcg gggttggatt agggatcatc tctggctact acggagggat
    37381 cattgacaac atcttgatgc ggatcgttga ttttatcatg attctaccga tcatgctgat
    37441 cattatcgtc ttcgtatcga ttatttccag atacaatgtc tggtccttta tattcatcat
    37501 gagtgcattt tattgggtag ctaaagcccg attattccga agcaagacat tatctgaagc
    37561 aagaagagat tatgtcagtg cctcaaaaac aatgggaacc aatgatttta aaatcatgtt
    37621 ccgcgaaatc atgccgaatc taagttcatt aatcattact aatttgacga tgaactttgc
    37681 ggcaaacatc ggtatcgaga caactttgac tttcttaggt ttcggcttgc cggcaaatgt
    37741 acctagttta ggtacattga tcggttatgc aagtaacgga gaagtcttag tgaatcggca
    37801 atggatctgg ttacccgcat caatccttat tttagttttg atgttgagta tcaattatgt
    37861 tggacaagca ttcaagcgtt ctgcagatgc gcgccaacgg ttaggctagt atagaagcga
    37921 agggggaaat aggaatgaag aaaacgattt ttggatttgc agcgctgatg tctgctgtcg
    37981 ttcttgcagc atgcgggggc ggaggtacag atacctctgg aaacaactct ggaggcaacg
    38041 gcaataacaa agaggaatta acttttccaa ttgcaacaaa aaataatgat gatgcaatcg
    38101 aagacggcga attagaagct gcggtcgcaa cggacagtca gtttaaagga ttgttccagt
    38161 gggagtttta ccaagattca tatgatgcag cttttatggc tccttctcat gaaccgctat
    38221 ttgcaaacga tgacgatttc agaatcacag acgacggagc tgcaacgctt gatctagatg
    38281 aagatacgaa aaaagcgaca atcaaagtaa aagacaacgt caaatggtca gatggtgaag
    38341 atgtaacggc ggacgatgtg attttccctt acgaaatcat tgggaataaa gattacactg
    38401 gtatccgtta tgacgacact ttccgtaaca tcgtaggtat ggaagaatat catgatggaa
    38461 gtgccgacac aatctccgga atcaaaaaaa tagacgacaa aacagttgaa atcgaatata
    38521 aagaagtaaa cccaagcatg cttcaatcgg gtggtggtat ctggtcatat gcagcaccaa
    38581 aacatacgtt agaaggtgta gcaatcaaag atatggaatc aagcgatcaa gtacgtaaaa
    38641 atccagtgac ttttggccct tattacatga gtaatatcgt tgcaggagaa tcagtagaat
    38701 acttgccaaa cgagcactac tggaatggta cgcctaaatt gaaaaagatc acgatgaaat
    38761 ctgtaccgat ggcaagtgca actgaagcat tgaactctaa attatatgat atgattttcc
    38821 aaatgccaac agatacttac gatacctata aagaaattgc tggctataca aacttaggcc
    38881 gtgaacaaac ttcttatact tatctaggat tcaagttagg taaatggaat gcagatacaa
    38941 agagtgtgga atacaatcca gattcaaaaa tggctgataa atcattacgt caagcaatgg
    39001 gctatgcatt agataataat gcagtaggtg aacgtttcta tgcaggtttg agaagcaatg
    39061 cgacttcatt gattcctcca gtattccagt cataccatga tgatgagatg aaaggattca
    39121 cacaagatct agacaaagcg aaaaaactat tagatgatgc tggctacaaa gatgtggatg
    39181 acgatggttt aagagaagac aaagatggaa ataaactaac aatcaacttt gcttcgatgt
    39241 ccggtgggga aacagcgcaa ccattggcag attactatat ccaacaatgg aaagaaattg
    39301 gtttggatgt ccaatacaca acaggtcgtc tgatcgaatt caattctttc tatgataaat
    39361 tagaaaacga tgatcctgaa attgatgttt accaagctgc ttggaataca ggaactgatc
    39421 caaatccaag tggactatgg ggaccaaatg ctgcgttcaa ctacactcgt tatgaatcag
    39481 aagaaaatac aaaattgatc aacgatatca gttcaagcaa agcatttgat gaagagtacc
    39541 aaaaagaagc atatcaaaaa tggcaacaat atgcatttga agaagcattt gcgattccaa
    39601 cgttgttccg taatgaagta ttgccagtca acgaccgtgt caaagactgg gattggtctt
    39661 atgatgtacc aaacccatgg gcaaatgttt ctgttacagc agattcacgt tcataatcac
    39721 taaaaatgac cgaaaaaggg cgatgtcctt tttcggtttt ttgatatttt tctgtttatt
    39781 tctctaatat tcaggtcaag gcgctgattt catgacttct ccaataaacg gtgttcaaag
    39841 tccagtttct cggtattaag tcaaatttat gcaaaaatat ggaaagccat tttcacaaaa
    39901 ttttttcttc atactcgaaa ctaaacgaat ttttcacaac ttctcgtcaa tcactaggat
    39961 tattaaagct ttacttacct tttgaccgaa ccatttcaaa tttgacaaaa aaccagtata
    40021 ataaggaagt atcgaaaaaa tatccataaa aggaatgtgg aaaatggaca accagctgac
    40081 aaaagaacta aaagaaaact ataacatcgt ttttcacgat ctaaatttgc ttgaacaagc
    40141 ttttacccat tcatcctatg tgaatgagca tcgcaatctg caattatcgg ataatgagcg
    40201 acttgaattt ttaggagatg ccgttttaga actgatggtt tctcagtatt tattccgttt
    40261 atatcctcat ttgccagaag gaaaattgac aaaaacacga gctgcgatcg ttcgcgaaga
    40321 cagcttgtcg aaatttgcca aagaatgtca ctttgatcaa tatgttttgt tgggcaaggg
    40381 agaagagaat tcaggaggac gtacgcgacc agcattgctt tgcgacctat tcgaagcttt
    40441 tttaggcgcg ttatatcttg accagggatt cgacacgacc catgcattca tcgaaaaagt
    40501 gatttttcct aaagtcaaag caggtgcttt ttcacatgag atggatcata aaacaaaact
    40561 acaagaagtc ttgcaaaaat caggtgatgt atcaatcgaa taccgattga tcaatgaaga
    40621 aggtccagct catgaacgag tattttggat cgaagtttat gtagatgatc aattgattgg
    40681 cactggacaa gggaaatcga aaaaattagc agaacaagct gcggcggaaa atgcattggc
    40741 agcactgtaa aagtaagtga gggaaagctt tgtatttgaa acgaattgaa atagccggtt
    40801 ttaaatcatt tgcagacaag acagtcattg attttgaaaa tagcgtcaca gcagtggttg
    40861 gaccaaatgg cagcggaaaa agcaatatta cagaggctat ccgctgggta ttaggagaac
    40921 aatctgctaa aagtctgcga ggcggaaaaa tgccggatat catctttgcc ggttccgata
    40981 cccgtaagca gctgaatatt gcagaagtca cagtgatttt agacaatagt gatcattatc
    41041 tgccattgga gtacaatgag atcagtgtaa cccgccgtta tcgacggact ggggaaagcg
    41101 agtttttcat caataaacaa agctgcagat taaaagatat ccaagaattg ttcttagatt
    41161 ctggattagg aaaagaatcg ttctcaatca tttctcaagg aaaagtagaa gcgattttca
    41221 gtagtaaacc ggaagaccgt cgtgggatct ttgaagaagc agctggtgtc ttgaaataca
    41281 agcagcggaa aaagaaagca gaacagaagc tttttgaaac agaagacaac ctcagccgcg
    41341 tccaagacat catccacgaa ttggaagaac agttaactcc tttggccgcc cagagtgagg
    41401 cagcaaagga gtttttacgg ctaaaagaaa cactgactca aaccgatgtc tctttgatgg
    41461 ttgctgaaat caagacagca aaaaaagatt gggacaacaa acaagcacag cttgcgaaat
    41521 tcaatctaga attaggcaaa ctatctgagt cgatccaaga acaagaatcc attttggcga
    41581 agcagcgtaa agaaaatgcg caagccgatc gtctgatcga aaaaaatcag caagtattat
    41641 tagatctttc ggaaaaactg aagcagacag aaggacaaaa agatgttttg caggagcgga
    41701 caaagcatac acaaaaaagc tcgcaagaat atcaaacttc attagcagaa gcacaaaaaa
    41761 aggttaaaca ttttgaaaag cttcaagaaa gcttgatgaa agcagcagca gaaaaagaaa
    41821 cagagatcca aaaagctgaa gcaaatctca taaaaacaca gcaagaatta gaaaaatacc
    41881 aaaaatcaac gaaagaatta ttggcagaat taagagatca atatgttgat ttgatgcaag
    41941 aacaagcagc tgtaggaaac gaactgaagt atctagaacg tcaatatata caagaaacag
    42001 caaaaagcaa gcagacgtta gccaagcaat cggaagtaga agcatcagta gaccgattga
    42061 tcttacaaaa agaagaattg acacaaaagc aggcgcagct aaaatcatct ttaactgaaa
    42121 caaaagagaa gttagaaatg atccaacaaa acgggaaaaa gttccaagaa gcattagcaa
    42181 aagaacagcc taaaatgtat caattaatga atcaagtcca gcagctacgt gctcgtaaga
    42241 aaagccttca agaaatccaa gaaaactatt ttggttttta ccaaggggtt cgattggtct
    42301 tgcagcataa acagcagctt tcgggtattg tcggagcagt ggctgaactg atcgatgttc
    42361 ctgcagattt cacgttagct atcgaaacag ctttaggtgg ggcagcccag catgtcatcg
    42421 ttgaaaatga aaaagatgct cggcaagcca tcacgtattt gaaacagcaa agaggaggaa
    42481 gagcaacatt ccttccactg acgacaatca aaccaagaca attaccggct catatattga
    42541 cacaagcagc agctgtagaa gggtttatag ggattgccag tgaacaagtc agttatcccg
    42601 atcaaatcca aactgtggtt caaaacctgc taggaacgat ccttttagcc aaagacttaa
    42661 ctagtgccaa tgcaatcgcg caaacgatcc gctatcaata tcgagtggtt tccttagaag
    42721 gtgatgtgat gaatgccgga ggatccatga caggtggagc gaataaacga ggaaaccaag
    42781 gcagtttgtt tgtacaaaat caagaattga aacagttgac tagtgaattt gaagaagcag
    42841 ataaacaact ccaagcacaa gaaaaaaaag ttcaggagct gcaacaagaa acagctcgtt
    42901 tagccgaaga acaagaagtt ttgcgtacac gaggcgaaca attacgtttt gaagaacaag
    42961 aagcgacaaa tcagctacaa aacatcataa atgaacttga acgttttgaa aaagaaaagc
    43021 aaatctcaac ttttgagaca cgtgaactgc agcagtttat cgaagattac gaaaagcaaa
    43081 cgaacgaatt gaaagacaaa caaacagatc ttgaatcgca acgacaaaaa atcgacgaag
    43141 aaatcaaatc gttaagtcaa gaaagcgacc agatggaagc cagaagagca caagtacaaa
    43201 gtcaaaaagc acaagaacaa gcagatcttg ctgtcttgaa agagcagttt aatcatctgc
    43261 agatccagct ccgcggtgcc cgagtccaaa aagcagaggc aaccgaacgt caagaagcga
    43321 tagaaaagca gttggcgaca ttgacagcag acttttctga tcatgaagtc acagaagaaa
    43381 gtttggaaaa acaaattaac gaattatctg ctcagcgaga aacattaaaa gccgagttgg
    43441 caaaagccaa agagcagcga gatagaaggc aaaaggagat cgataagctt gaagcagtct
    43501 tggcagagcg taatcgtgag caaaaagcac gactgtcgga acaatccaaa ttagaagtcc
    43561 aaaaagaccg agctgaaatg attttagata atcatttgac ctatctgcag tcagaatatc
    43621 agctgacatt tgagaaagct tcccaagact atcaagaaac aaccgatatc gaagacagta
    43681 gaacaaaggt ttcatcctta aaagaacaga ttgaaaaact gggaccagta aacttaaatt
    43741 caatcgaaca atacgaacaa gtcagtgaaa gacatacatt cttagcaaca cagcgagatg
    43801 atctgttagc agcgaaaaac caattgtttg aaacgatgga tgagatggat gatgaagtgc
    43861 ggacaagatt caaagaagta tttgaagcta tccgtcaaga attcaaaatt gtctttccaa
    43921 atatgtttgg cggcggtaaa gctgaattag tactgacgga accaactgat ttgctgaaaa
    43981 caggtattga gattgaagta caacctcctg gcaaaaaact gcaaagtcta agtttgcttt
    44041 ctggaggaga acgagcactt acagccatcg cgttgctctt ttccattatc cgtgtctgtc
    44101 ctgttccgtt ttgtatctta gatgaggtag aagcagcgtt agatgaagcc aacgtgaaac
    44161 gttttggtcg ttacttgagc gatttccaag atgacacaca atttatcgta gtcacacacc
    44221 gcaaaggaac gatggtcgca gcagatgtac tgtacggagt gacgatgcag gaatccggtg
    44281 tatcaaaaat cgtttctgta cggatggaag atatcaatga agaggcgaaa atcaacgaaa
    44341 cgaaagtgta ggaacgaaaa tgatcaaatt agtagcaatc gatttggacg gtacattatt
    44401 aaatgaacaa aagctgattt cagatgagaa taaacaagca ttagcacaag caaaacagca
    44461 aggggtcaag atcgttctat gtacaggacg ccccttagct gcgatggcgc attatctgca
    44521 agaattagga ctggtagatg aaggtgacta cagcatcact ttcaacggcg gtttggtaca
    44581 aaaaaatgat actggtgaaa tcatggaaaa gaaagtgatg gaagtatcag atatccaacg
    44641 cttatacaaa ctggctcagc aattggatct gccactagat gttttgtctg acaatgtggt
    44701 tttacagctg cctagtgccc caaggaagca atccttgtac aatgtgttga ataagctgct
    44761 gcaattccaa ccagcggata taagcgatat tacagatgaa tttgtgctta ataaggcagt
    44821 aatcgcttat gaccaagaag aattagatcc taaaatcaaa gaaataccag cagaattcca
    44881 tgaaatgtat gagatcatca aaacaagaag tatgcttttg gaatttatgc caaaaggtgt
    44941 tacaaaagct tatggtattt ctttattggc aaaagatctt ggtttgaaac aagaagaaat
    45001 catggcgatc ggtgatgagg aaaatgattt accgatgatc caatatgctg gactaggcgt
    45061 agcgatggag aatgctgtgc ctttcgtcaa agaagcagca gatttcgtga cagccagcaa
    45121 tgaagaaaat ggcgtagcca aagtgatcca aaaatttgta ttagagtagg gggagtcaaa
    45181 tgggattttt tgataaaatc aagcgtgctt ttactggcga agagaaaaca gaagaagttg
    45241 aagtacagga gaagtacgac aagggtttag aaaagacgcg taaaacattt ggtgaacgga
    45301 tgaatgagtt gtttgccaat tttcgaacag tcgatgaaga cttttttgaa gaattagaag
    45361 aaacacttat cggtgcagat gtaggttttg aaacagcgat acgaattacc gaagcattac
    45421 gtcaagaagt gaaactcaga aatgcgaaaa aatcggcaga agtccaaaat gcgatcatcg
    45481 agaaacttgt ggatctctat gaagcggaag gaatcaatga agtaaatgaa ctaaatatcc
    45541 aagaagacgg actaacagtt attttgtttg tcggtgttaa tggtgtaggg aaaacaacaa
    45601 gtattggtaa acttgcccat cagttcaaaa acgaaggtaa aaaagtattg atggcagcag
    45661 cagatacctt ccgcgctgga gcaatcgacc agttagttgt ctggggcgaa cgcgctggtg
    45721 ttgaagttgt tcgtggcaat gcaggaggcg atccagcagc tgtcgtattc gatgctttgg
    45781 aaagagcaaa gacagagcag gcagatgttt tactagttga tactgcaggc cgactgcaaa
    45841 ataaagtgaa cttgatgaac gaattagaaa aaataaaacg agtaatccag cgggaattac
    45901 cagaagcacc ccaagaaaca cttcttgtat tagatgcaac cactggtcaa aacgcgatgg
    45961 ttcaagcaaa acaattcaaa gaaacaacag atgtgaccgg tcttgttttg acaaaattag
    46021 atggtacagc aaagggcggt atcgtacttg ctatccgtaa tgaattgcat ttaccagtga
    46081 aattggtcgg tcttggtgaa ggaatcgatg acttggagcc gtttgatcca aatgattttg
    46141 tcgttggatt gttcaaagga ttactaaaag aagaataacc catgtctaaa aagcaggagc
    46201 tgtgacaaaa gttgcagctc cttgtttgaa tgattgtttt taacatagct gtgtttttga
    46261 tgccttcatt tttatactta tttgtaagcg gttctcaaac aatttcgttc tttttcttag
    46321 actttttgat tataattaga ttcatatgaa ttggaggtca cttaatgatg aattttaagc
    46381 aattattatt gcatgtcaat gcgcgccctt ttatcgacca agctcgattt ggtatagaac
    46441 gggaaggaca gcgggttgat cttgcaggaa atctagcaaa aaccgatcat ccagcaatct
    46501 ttggcgatcg atcctatcac ccctatatcc aaacagattt tagcgaaaca caaacagaga
    46561 tgatcacccc tgttaccgat tctattcccg aattatttca gtatctcgct gctgtttatg
    46621 atgtgactgc tcgttctata ccgaaagaag agatgatctg gccattaagt atgccacctg
    46681 ccttaccgga aaaagacgaa gagatcatta ttgcaaaatt aaaaaatttc gaagatgtct
    46741 tgtatcgacg ttatttagca aaagaatacg ggaagcgaaa acagatggta agcggtattc
    46801 atttcaattt tgaatttggc gatgagttgc taagaacatt gttcagccat caggaagaat
    46861 ttcaagattt ttccgaattc aaaacagaac tttatttgaa aacagcgaga aactttatgc
    46921 gctatcgctg gatgatcact tatttattcg gtgcttcacc aatgagtgag aagaactact
    46981 ttttagatga atcacatccg caagaacctg ttcgcagtat tcgcaacagt gcgctgggct
    47041 atacgaatca tccaaatgtg aaagtttcct atgcttctat gaaacagtat ttagcagata
    47101 tcgagcgaat gatcgaagaa gggaaacttt cggaggagaa agaattttat acaccgcttc
    47161 gtttccgagg cggaaagaaa gtcgcagatt tagcaacaac aggtgttcgt tatattgaat
    47221 tgcggaatat cgatttgaat ccttatgcaa gattgggtat caatccagag caagttcggt
    47281 tcttacaatt attcctgatg tacatgttat ggacagaaga aaaagaagac tgtgaccaat
    47341 gggtagcaga aggtacgact cgaaataaca aagtagcact agaacaacca tcagatcaaa
    47401 cagaatttca tcaagaaggt agagaaattc ttgaaggaat gaagcaaatg ctggttgaat
    47461 tggattggtt ggattctctt tatttagtag aggaagcatt gactcaaatg gatcatcctg
    47521 aacaaacgtt agcagcgaaa ctctatcaag aagcacagct atcgagtcag caagaagttg
    47581 ctgtggcatt gggacatcaa tattataaag aaagccatga acgtccctat cagttggccg
    47641 gttttcgcga aatggaactt tctactcaga tatttatgtt cgatgccatc caaaaaggtg
    47701 tccaagtcaa agtattagat gagtcggatc aatttttgcg tctgcaattc caagaccatg
    47761 tagaatatgt gaaaaatgca aacatgacaa gtaaggacag ctatatcgtc ccacttatca
    47821 tggaaaacaa aacagtaacg aaaaaagtct taaaagaagc tgggttccgg gtaccaggag
    47881 gcgcagaatt ttcatccatg gaagaagcgg taaaagctta tccgagattt gccgagcagg
    47941 catttgtgat caaacctaaa tcaaccaatt acgggttagg tatcacgatt ttcaaggaag
    48001 gagctagtct ggaagattat caagcaggtt tagccattgc ttttcgtgaa gacagttcgg
    48061 ttttagtaga agaatttatg ccgggaacgg aatatcgttt ctttgtgatc gacggagaag
    48121 tccaagccat catgttgcga gtccctgcta atgttattgg tgacagtatc cgaacagtga
    48181 aagaacttgt tgaagaaaaa aatagtgacc ctttgcgtgg aacgaatcat cgtgcacctt
    48241 tagaattgat ccaattaggt gaattagaac aattgatgtt gaaagaacaa gggttgacga
    48301 tcgaatcagt tccccaagcc aatcaaattg tttatctaag ggagaactca aatatcagta
    48361 caggtggtga ttcgatcgat atgactgatg aattttctga agcttacaaa aaaatcgccg
    48421 tttctgcggt agaagcacta ggagctaaga ttagtggtat cgatttgatt ataccagata
    48481 aagaaatcga tcctacaact gataaaaaag catatgggat catcgaagca aatttcaatc
    48541 cagccatgca tatgcatgtt tatccttttg caggaaaagg cagaagattg acgatgaatg
    48601 tattgaaatt gttatatcct gaagtatttt aaaagaaaaa gcttttgaca gattgtccta
    48661 tttgatgtca atctgatcaa aagctttttt tgcatgtaaa aatttatgtg ttttttcgtt
    48721 ttaaaataaa cgatcccaga agcttttttt cttttttggt ttttcttttt cagctggcga
    48781 agagttttct tctgggtatt ttttttcgat atcaatcaca ggttcattta ctgcttcttt
    48841 tgctgttaag agcaatccga attcctcagg atttgcagtg acgtaatcgt tgactacagt
    48901 gaaacggcta ttgtttttcg ttgctagctg gatatattgg ttttggacag aaaggggtag
    48961 agccccattc aagagtatgg aagcatctgg atgttttttg agttctttta aaaagttttc
    49021 tttgtttttt ggttggatca tttcagcaac agtcatacta agatagcagc gttccctgaa
    49081 tgtccctaaa tatttatgct gttcttcagg gttcactaaa ggtgtaccat aaagtccctt
    49141 atccaaatgg ctttttacat catcttgtgc cataaaaaat accctccttc ttctctttgt
    49201 ctttcttacg attatagcat attcccaata atacctaaaa catatgaaaa agcggttgct
    49261 tttgttcaac gattcactta aaataagaat gaaaatcatt ctcaattaga aaggtggatt
    49321 ggatgcgcta cggacgacta gtagcattat tagttatttt aagtagtatt tcattgttca
    49381 tcggagtatc gaatgtacaa ttgtcagaac tgctccagtt agatgggcaa agcttacaaa
    49441 tcttgctagt gactcgtttg ccacgaacga tttgtttgat ccttgtggga gcgaccagta
    49501 gtatttgtgg gttgatcatg caacatttga cccaaaataa atttgtttca ccaacgactg
    49561 cgggcacgat ggatagtgcg cgtttaggta ttttagtggc tatgattttc ctacctggtg
    49621 catccttgat tgtccgatcc ttgacagcat tttgctttgc ttttgcagga acattgcttt
    49681 ttttatccct tacgcgattg ttcctccaga aaaatcaatt gatgcttccg ttgattggag
    49741 tcatgttagg gaatatcatt ggttcagttg ctactttttt tgcttatcaa ttccagttgg
    49801 tacaaaatat gacttcatgg cttcaaggga attttgcgac tgtgatacga ggaaattatg
    49861 agttattgta tgctgttgtt ccattgttgc tcttggtttg gctatttgct taccgattta
    49921 cgatcattgg tctgggcgaa tcgtttgcag taaatctggg aatagattac cagaagatgc
    49981 agtttttagg cattggctta gtatcattgg ctagtgcagt catgttgatc atggtaggaa
    50041 gcattccttt tatcggagtg atcattccga atcttgtctc tagaatatat ggagatcaag
    50101 tgcacaaaac aattggcgta actgctttag caggtagttt gttcttagta ttttgcgatc
    50161 ttgtcgctcg cgtgttgatt tttccttatg aaataccagt cagtgtgatc gttggaatca
    50221 tcggtggggc tatcttcctt tatcttctga taaggggacg acgatatgct taaaaaaaag
    50281 ccatctattt ttattggtat gcttgttctt gttttatttg ttacaggaat gtacttgacg
    50341 atccatacat atggaaactg gcattttgca tgtgcattga ggagtaagaa aatttttgca
    50401 tttatccttg tctcgctagc tgtcagcagt gccacaatca gctttcagac aattacgcat
    50461 aaccagtttt tgactccttc agtattggga cttgagtcgt tatatgtcct tgtccaaacg
    50521 gtccttttct tctttatcgg aggagtgacg atgcttcagc agacaggtac agtttttttc
    50581 ttattgaacg ttgggctgat ggtcgggctg agtctattgt tatttttctt tcttttgaaa
    50641 aaaatgagag cgaatctttt tttattactg atgtcaggga tgattttagg tacgcttttt
    50701 ggtagtgtca gtaccttttt acaagtcatc atggatccga atgaatatga tttgcttcaa
    50761 ggcagattat ttgcaagctt tggcaatatc cagagtcagt atcttttgcc tgcttctttg
    50821 atcattatcg gctgctggta cctgttattt cgatatcgac atgtgctaga cgttttccat
    50881 ctcggaaatg aacaagcagt aaacctcggg atagacacag atcgttttga atttttgatt
    50941 ttatgttgtg tcagtctttt gacagggaca tcaacggctc ttgtgggtcc agttactttt
    51001 ttagggttta ttgtcgcaaa tatcagttat cgtgtctttt ctacttatcg tcatggtcag
    51061 ctatttatcg gaagctttct ggtagctatg ctgtttctga caggaggaca gctcgcagta
    51121 gagcagattt tcggctggaa tacaacagtg agtgtcgtcg ttgaatttgt cggaggaatt
    51181 tattttgtca caaaattgat tcgcgaaagg aagagtatcc tatgattgat ttacaaaagg
    51241 tcaataaact gtaccatgga aaatcagtgg ttaaagatgt acagttaacg attcctgaaa
    51301 agaaattgac tgcttttatc ggtccaaatg gtgctggaaa gtcaacggtg ttgtcgatga
    51361 tcagtcgttt gataccaaaa gatacaggtg aaatctattt agaccataac gaagtaaagg
    51421 cttggaaatc caatgaatta gccaaaaaat tatcgaccct acgacaatcg aatgaaataa
    51481 atgtccaaat caccgtgaaa gaattagtag aatttggacg atttccttat acgaaaggaa
    51541 aattgacgac aaaagacgag gaaatcgttc aggaatcact ggaacacctt gggttagaag
    51601 aaatagcgga tcaatcgatt gacactcttt caggcggtca actccaacgt gcgtatattg
    51661 caatggtgct agcgcaagac acggattata ttttactgga tgaacctttg aacaatctag
    51721 atatgaattt tgcggttcaa atcatgcaga ttttaaaaaa tttagtacaa gaaatcggga
    51781 aaacaattgt tattgttctt catgatatca actttgctgc aagttatgca gacgaaattg
    51841 ttgcaatgaa agatggacag ttatttacgc aaggaacgac ccaagaaatg ataaaaaaag
    51901 atgtattgga tgaattatac gaaatggaca tacggatttg tgagattgaa ggccgcagat
    51961 tctgtcttta tttctaaaac taacattagg agagaagaga tgaaaaaatt agtagtaggt
    52021 atcttgattc tagcatcatt tggtttagca gcatgtggaa atagcaacaa caccagtcag
    52081 gcagacacaa aaaaaagcag cacacaaact gaaactacat tgacgataac agatagtaat
    52141 ggagaccaaa tcgaggtgcc aaataatcca gaaaaagtcg tcgtattcga caatggttct
    52201 ttagatacga tggatgctct tggtgttgga gataaggtag ttggagcagc tacaagcagc
    52261 cttcctgagt atctttcatc ctataaaaaa gtagaatcag caggtggaat aaaagaacca
    52321 gacttagaaa aaattaatca gcttcagcca gatctaatca tcatttctgg ccgacaaaga
    52381 gattttcaaa aagatctaag tcaaattgct ccgaccattt tcctttcttt agatgcgaaa
    52441 aatccatggg aatctttcca gcaaaatgtc actgctttag gggagatctt tggtaagcaa
    52501 gaagaagcta aaactcaatt agaggaatta tctagtgcaa tcgatcagac gaaaaagaaa
    52561 gcagaagcta ctgacaaaaa agcactagtc acacttgtga atgaagggca attatcagcc
    52621 tacggatctg gttctagatt cggtttcatc catgatttat ttggctttga acaagcagat
    52681 gatcaaatcg aagcatccac tcacggtcaa agtgtttctt atgaatatgt gctggaaaaa
    52741 aatccggata tcttatttgt agtagacaga acaaaagcta ttggtggaga cgacagcaaa
    52801 gatgatattt ctgccaatga actggtagca caaacgaatg ctggtaaaaa tcagcagatc
    52861 atctcccttg aaccagatgt ctggtattta agcgggggcg gtttagaatc aatgaaactg
    52921 atgatcgaag atgtgaatca agcatttaaa tagcataaat aacagaggat taaactctca
    52981 tactatgtaa caataatgtt tcttacattc ttaaatagca agagcctatc ctcaaagaag
    53041 ttatcaaaaa ccatccgtaa aatcgtattg atgattttac ggatggtttt tatttatttg
    53101 agaaatcttt tttcaacgtg aattcattat ctacatcata cgggttgatt ctagaatggg
    53161 gagtggcaaa aaatagtgtg atccattttt tcagcatcaa gatcgcctcc tttgatttga
    53221 atcaaaggta caaagcgaaa cttagataaa acttaaattt agatattttt attttgtacc
    53281 gatctcttct ttaaacgatg ttccatctgc tgcttctcgg caataagtcc actagactag
    53341 aaaatatgag aagctatttt ctgtccagcg ttcttattac tcaaagcaaa tcgcagttgt
    53401 cacaatctct tctaaacaat gttcaatcag ctgaccttcg gtattagctc aaagcactga
    53461 tttcacaacc tctatttagg catgtgtctc agcaagcggt tgatagttga tgatctgcat
    53521 ttttcgataa aaatcggcgg gaagcttact ccaccgtttt aataattgat agaaataact
    53581 ctcttctgct ttgattttat actcgcttaa tatcaatcca aaactattcg ttttttttga
    53641 agctaatttt ttataaatat ccaatgcttt ggttttatct tcattgacaa agagtgtttt
    53701 tacttcaata taagaaccct cgatattttg tgtttcttcg ataaaataga tgttttttat
    53761 atattccttc atagtgattc ctccaaagaa gtcttttatt tgataagttt actctaccga
    53821 agagttgtta ataatccgtg aacaaaaagt aattcttttt ccaatagttg ttaaaggttt
    53881 tttatttttt atagtatttt ttgttattta agaaaggaat aaggttaaac aaataacagg
    53941 ttgctttttg ggtctttttc cgttcaagaa actgaacgaa gaaaagggaa agttgtctct
    54001 tttgtgataa gagaaatcat aataaacgaa tgacttactt tccaataaat tgagaacagt
    54061 cagaaccaga caaattagtt gcatctgatg aataaagtga ttattctatg acatgaggtg
    54121 atacagatga aattagaagt gacaaaagaa gcacaagaag tattgaagaa taaattagaa
    54181 gcagacgatc aacttttatt agatatcgaa aacggtgacg gtccatttgc agaaagccaa
    54241 gtcagttgcc aattggatac ggcatttcga ttgatcattg tcaaaaaaga tacacaaaca
    54301 gatctaagtc tttattctgt agtggctgat acaccagttg gaccaatcaa aatcaaagac
    54361 agtgcaatac tttacatgga tgatccatcc acacttgctt tagaaccaac ttataatagt
    54421 ttacaattaa aaggaccaag cggcttgaga aaaggcaact tgcaagttgt tcgaaaagac
    54481 tagagggata aaatgggaaa attggcgatc atcagcgacc tacatgtgga catcaataaa
    54541 ttcggcgaat cagagctgat gcagctttgg caagttttgc aaaagcacaa gatcactcgg
    54601 cttcatctag ccggagatac ggctaataaa gtagaccgtt gcctagctgt tgtcgaattt
    54661 ttcgtcaata aaggattatc tacgacattt catttcggca atcacgagtt ggcagatgtg
    54721 cccaatgaac agatgatgga ccattttcca gacccacatt ttttaaatga acggtatatt
    54781 gctttaaatg agcagaccgt tcttttaggg atgaatggct ggtacgacta tcagttttcc
    54841 gaattgaaag attacgaggc aatcgtacga atgaagcgtt tgtattggta tgatcggttc
    54901 atccagcgtg ggaaaacaga tctcgaggtc aatgcagaaa tgcttgcagc tgcaaaaaat
    54961 ttgctagacc agctggaagc aaaaaaactt gaggtgattt tatccactca tttcgttcct
    55021 aaacgggcat ttatcatgta tcaatctgct ccttatgagc gttggaacaa attaaatgca
    55081 tttttaggat cagaatcatt cggagaatta cttgaccgtt attcaaatat caaacaagta
    55141 gtttttggcc atacgcatcg tcgctttgaa gataaatcca tcggtcacac gatttacagc
    55201 tgcagacctt ttggttatta ttatgaatgg cagctgaccc gagattttgt cttgaaagaa
    55261 cagttgctag acagctatga tcctatgaaa ttacgtgtcc tcttgcgtgc ccatcaccca
    55321 gcttttcagg cctatcaaac acaacatttg caagaggaat ttgaacaagc gatgacaatc
    55381 gttccgtatt gagacagcat tagtaatgtg taaaagaaaa taccttgtaa aacataagga
    55441 taagtaagag cagtagatcc atttatcgtg gatatgctgc tttttttgcg gaaaataatc
    55501 agcgaatgtt tatttgatcg gatgatttgt aactcgcaga gtactagatg caagcgtgta
    55561 ttcttttttt gaacgataat cacaaaaaaa aatataatat acatttttta tttaattata
    55621 attaatattg attgaagtct aggcagtcaa gtcacatttt gatagatagg agggggactt
    55681 acgaattatt ttaaaggcta tgacagctgt atttctagat tgtgtttgca tcaagttgca
    55741 ataaaaacta gaaatcaaat cgggctgata atcatttata actaatcgat acataggacc
    55801 aaatataggt ctatgttctt ttatttcata agtatcgtaa aacagaattt tgctatggat
    55861 taatccaatc gactgatatg tttagtaaat aaagaaagag gaatgtcaat gatgaaaaaa
    55921 gaaatcgtat taacagcagc aatgatgtta ttcagcatgg tagcaagtac gacttttgtt
    55981 tctgcaactg aagtataccc aaaggaatat aacacagaag gaacgattac atttgaagca
    56041 ggagatgagg gtgtaactcc tcctgttgat ccagagaatc ctgatcccaa taaacctgtc
    56101 gatccatcag atcctccaag ccctggaaca ggaggggcat tatcgattga ttatggatca
    56161 aaattcaaat ttgggactca aaaaatttct actgcggata aaacgtatta tgcagctgct
    56221 gatgtgatga acgatgggtc tagaaaaccg acatatgtcc aagtgacaga taggagaagt
    56281 accttatctg ggtggaaatt aagtgtctct caaccagaac agtttaaaac tgccagtggg
    56341 gatgaactag ttggagcaca gctgaaattt acgaaagggc aggctgtttc tttggttgat
    56401 ccaacatata cgccgcaaac ggtgaattct gaattaacgt tgacaccggg aggaaacaat
    56461 acgttagcaa ttaatgctaa atcaggaaca ggtgtgggga cttgggtcta tcgatttggt
    56521 gcaaatgaaa atgaaaatca agatgcggta cagctttcgg tacccggtaa atctgtcaag
    56581 ttagcgcaac aatattccac taaattagtt tggacattag aagatacacc taacaactag
    56641 tttactgaaa agaactgtta aaaataaata cgtagtcata ttgtcttgcg aggtaatcga
    56701 gcaaaaattg tcaaaggcta ggctaaattt gtttggatac gaatagttca tctaggaatt
    56761 taacgatcag gaggaaaaga tatattgaaa aagaggagga tcgtgctcat tttaagcagt
    56821 ctcttagtga ctaatatctt tccgatcata gggtactctt tggaattgaa tcagttggag
    56881 catggaaata gaagagacga agaaagcaaa ctcacttcga cattcgacag tttaatagaa
    56941 tccaaccaga cacaagaaag cagtgaggat ttaagtattt cgagtagtgg agagaccact
    57001 gacaacgtag aagctgaggg ttcggacaaa caaggactag aagatgcacc aatttctgcg
    57061 gttgagctgc ctgacgatcc caatattatt tctatcgata aaatttttca aacacctatc
    57121 ggtactagca cgtccatatt ggaaaatggg aagttactgc agttgaaccc agcagaaagg
    57181 tctcaaagag gagctatttg gtcaaataag aagattagtt tactctcgga ttttacattt
    57241 aaatcttatt tatacttagg aaatcaacga gatagagcgg gagatggcat gacctttaca
    57301 ttgactggcg atcctagaat ggctagtaat cccagtcaag ttattggatc gccaggtatg
    57361 ggaattggct catattctac tcgttctggc caaccttaca ttgcgaatgc gctctcgatt
    57421 gagtttgata cgtataaaaa tagtggttct tctgatcgaa tggatcgaga gataactgct
    57481 gataatagag ggcatggaca tgtcgctttt gtcacgccta aaccaaataa taataattat
    57541 tctggagagc atactggcgt gacggtagct cctacatttc tttctaatgg cacatggcgc
    57601 ttgttaataa ttaaatggga cgcggagcaa agaagtctta cttatgattt ggaaggtgtc
    57661 ggtcactctt ctcggattat acctgatctc attaggcagt ttggttctac agaagtaaat
    57721 tgggggttta cttcttcaac aggtactttt tttcaagaaa atgctgtcgc tgtcacgcga
    57781 cttccatcga atgtcacatc aacagctgat gtaaaagtca acgatagtga ttatggggcc
    57841 atcgctgaag ctggaggaaa cgataagata actatcagaa atacattgaa ggtcgagggg
    57901 gattttcttg aatataagaa aatgcagact agtatcgacc tcccacaaga attaactatt
    57961 cccgagcata tcttgatcaa tggaaatcaa attgatgcca aagatatctt gataaccgga
    58021 aatcgagtca cgctaaatct gacagattat tttattgctg gagatacgtt aataatcgaa
    58081 ttggagacaa ctttgcagga agtaattcct gaaaaagtac tcactatgca ttttgaatac
    58141 cttgaaaacg ggaatttgat tgagcaatcg aatgaaataa acctaaagat cgcaaagctg
    58201 aaggagatag atatccatgt ttattataaa gataaagaca gtcagattga tattgctgaa
    58261 atgaaaacaa tcactgggaa aataggagag aagtataaag aaactcctat tgatattgaa
    58321 gggtatgttt ttgactcgga ttcagcgaat acagaaggca tcttctccga aaatacagag
    58381 gatatctatt tttattacag aaaaggcaaa ctttatctct caactgctcc tcaatatgtc
    58441 aattttggaa aacataaaat atccagtaga ccattaagtg aattcggaca gttttcaggt
    58501 gggttgaaaa ttatagatga acgggctttt ggaagttgga aattacaatt gaaacaagag
    58561 aaagttctta caaatggaga ttttgagctt ccagaagctc tttcctttgt tacctctgaa
    58621 aatacaacgg ttatagggac atccgctgtt acgattttcg aaagtgatca aaagggtgaa
    58681 agcgatttgt cctctctttt agattctaca gctcatcgtg gaatcagaat agatattcct
    58741 gtagaaaacc aaagaatcgg aacctttgag gggaaactat cttggatact tgcagatgtc
    58801 ccaggaaatt aacaggaaga gaagaatata acaaaattat tgagtttcaa gaagggcaca
    58861 ggtaccatag gctcttgtgt cccacagaga caagattttg ttatagtctt ctcttacata
    58921 tgattaaagg agcgattttc ttgagaccaa aacaacaatt atttataaca ctcctagtta
    58981 ttttaggggc actttttaac aatatatcag tgtcagctag tgcatttaat ttttcagtga
    59041 cgccgataac ttcagagaat caaatcgaca agagaaaaac ttacttcgat cttcaacttg
    59101 atccggatca ggaagtagaa gtaaaagcgg aattgagaaa tgatacagaa aaagaagtga
    59161 aaatcgatat ttctgtgaat agcgcaacca ccaattcaaa tgtgatggtt gaatacggaa
    59221 aaaacgagat tgaaaaagac atgagcctga tctttgatct ggtagattat gtgagctatc
    59281 cacagatagt gacgctaaaa cctaaaagtg tccaaaccgt tgcttttcat gtcaggatgc
    59341 cgaatgagcg atttgatggt gtattggctg gtgggatcac attccaagaa atccagacgg
    59401 aaaaggacca aacagaaact aaggatcaaa gcttatcgat tgagaatgaa tacacctata
    59461 ttattgcttt gttgatgcaa cagacactga acgaagttgc tccgaacctt actcttcatg
    59521 aagttaaacc agatcaaatc aatgcaagaa atgtcatctt agcaaacgtt caaaatgatc
    59581 aaaaaaccta tatcaatcaa gtagttattg aaacaaaaat tacaaaaaaa ggacattctg
    59641 aagttttata tcaagaggaa aaagaaggat tgcagattgc gccgaataca aatttttctt
    59701 ttccaactgc tttaaatggt caaccgctaa ccccaggtga atatcacctt acaatgactg
    59761 ttttaggaaa tgaaaatgaa aatggaaaat tttcgagaaa aaaaggaaat actacgatca
    59821 actataccaa tcaatgggta tttgaaaaag attttacaat agacggaaaa gtagccaagg
    59881 aattgaacac gacggatgtt acgattaaaa aagacaataa ttggatttat atcttagtag
    59941 cttcactttt cttattgttt attttattat gcatactttt gtttgttttg agaaaaaaag
    60001 aagagaaaga aaagtaaggt taagataaca aatagtaaat ttttattatt gtaggatggt
    60061 tgggctcttt ttataacaat ataaaatata ccattttatt aataaaaatg catactagta
    60121 aatgatataa ttctaataac aggtttgatc gcgagaaagg ccaaataaaa cgctctccaa
    60181 aatgttaaaa aaaagacagg acgatgtata ttatacaaac aagaatcgct gacaacagtg
    60241 actcggatcc aggaaatcag ttttatcccc agctagttgg tgaatgcatg gaaagtaata
    60301 tacgtagaaa gagctgatga ctaatgaatt atgagttttt tttaagcaaa gaagatcaca
    60361 gaagttattc attacttaaa tatttagaag caagctcaaa tttatcagag tcaatatcga
    60421 atgttcaaga agagttgtcg ctatcgacat ttctcttgaa aaaaacgatc gataagctga
    60481 aacatgattt acaaaagttc gatctagaga aaaatttgcg agtaaccgtc agtgaattgg
    60541 atatatgtct ggaaattaat ggaaaatgtt ctagcaaagc tctgttatct cgttatataa
    60601 cggattcttt atctatgaaa atggtattgg ctttctttca ggaaaaatat atctcagtag
    60661 aaaattttgc taaacagcat cacgtgagtt attctgtggc ttataaagta ttacaaagac
    60721 taaaaagaaa tctaaaaaaa tatcaaattt tttttgaaaa gagaaagttg a
//
LOCUS       DAZKSU010000008        59221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000008 DAZKSU010000000
VERSION     DAZKSU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.008"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(451..1761)
                     /locus_tag="ACG8Z3_000527"
     CDS             complement(451..1761)
                     /locus_tag="ACG8Z3_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0822875.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="ACG8Z3_000528"
     CDS             complement(1773..3191)
                     /locus_tag="ACG8Z3_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0822876.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="ACG8Z3_000529"
     CDS             complement(3431..4999)
                     /locus_tag="ACG8Z3_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0822877.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="ACG8Z3_000530"
     CDS             complement(5003..5413)
                     /locus_tag="ACG8Z3_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0822878.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="ACG8Z3_000531"
     CDS             complement(5410..6333)
                     /locus_tag="ACG8Z3_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0822879.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="ACG8Z3_000532"
     CDS             complement(6330..7208)
                     /locus_tag="ACG8Z3_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0822880.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="ACG8Z3_000533"
     CDS             complement(7195..8532)
                     /locus_tag="ACG8Z3_000533"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0822881.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="ACG8Z3_000534"
     CDS             complement(8535..9638)
                     /locus_tag="ACG8Z3_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0822882.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="ACG8Z3_000535"
     CDS             complement(9712..11220)
                     /locus_tag="ACG8Z3_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0822883.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="ACG8Z3_000536"
     CDS             complement(11186..11878)
                     /locus_tag="ACG8Z3_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0822884.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="ACG8Z3_000537"
     CDS             complement(11900..12874)
                     /locus_tag="ACG8Z3_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822885.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="ACG8Z3_000538"
     CDS             13174..14046
                     /locus_tag="ACG8Z3_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0822886.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="ACG8Z3_000539"
     CDS             complement(14504..16702)
                     /locus_tag="ACG8Z3_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0822887.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="ACG8Z3_000540"
     CDS             16934..17926
                     /locus_tag="ACG8Z3_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0822888.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="ACG8Z3_000541"
     CDS             17986..19407
                     /locus_tag="ACG8Z3_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0822889.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="ACG8Z3_000542"
     CDS             19400..20899
                     /locus_tag="ACG8Z3_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0822890.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="ACG8Z3_000543"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="ACG8Z3_000543"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0822891.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="ACG8Z3_000544"
     CDS             22491..23174
                     /locus_tag="ACG8Z3_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0822892.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="ACG8Z3_000545"
     CDS             23176..24012
                     /locus_tag="ACG8Z3_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0822893.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="ACG8Z3_000546"
     CDS             24023..25777
                     /locus_tag="ACG8Z3_000546"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822894.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRIYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="ACG8Z3_000547"
     CDS             complement(26053..26394)
                     /locus_tag="ACG8Z3_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0822895.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="ACG8Z3_000548"
     CDS             complement(26462..28633)
                     /locus_tag="ACG8Z3_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0822896.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28844..29701
                     /locus_tag="ACG8Z3_000549"
     CDS             28844..29701
                     /locus_tag="ACG8Z3_000549"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0822897.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="ACG8Z3_000550"
     CDS             complement(29772..30629)
                     /locus_tag="ACG8Z3_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0822898.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="ACG8Z3_000551"
     CDS             complement(30614..33316)
                     /locus_tag="ACG8Z3_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0822899.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="ACG8Z3_000552"
     CDS             complement(33329..34618)
                     /locus_tag="ACG8Z3_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0822900.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="ACG8Z3_000553"
     CDS             34768..35814
                     /locus_tag="ACG8Z3_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0822901.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="ACG8Z3_000554"
     CDS             35816..37969
                     /locus_tag="ACG8Z3_000554"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0822902.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="ACG8Z3_000555"
     CDS             complement(38442..39893)
                     /locus_tag="ACG8Z3_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0822903.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="ACG8Z3_000556"
     CDS             complement(39890..41614)
                     /locus_tag="ACG8Z3_000556"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0822904.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="ACG8Z3_000557"
     CDS             complement(41607..42221)
                     /locus_tag="ACG8Z3_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0822905.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="ACG8Z3_000558"
     CDS             complement(42566..44023)
                     /locus_tag="ACG8Z3_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0822906.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="ACG8Z3_000559"
     CDS             complement(44064..44984)
                     /locus_tag="ACG8Z3_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0822907.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="ACG8Z3_000560"
     CDS             complement(44996..45943)
                     /locus_tag="ACG8Z3_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822908.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="ACG8Z3_000561"
     CDS             complement(46422..47141)
                     /locus_tag="ACG8Z3_000561"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0822909.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="ACG8Z3_000562"
     CDS             complement(47190..48836)
                     /locus_tag="ACG8Z3_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0822910.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="ACG8Z3_000563"
     CDS             49015..49605
                     /locus_tag="ACG8Z3_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0822911.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="ACG8Z3_000564"
     CDS             49697..51202
                     /locus_tag="ACG8Z3_000564"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0822912.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ACG8Z3_000565"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="ACG8Z3_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0822913.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="ACG8Z3_000566"
     CDS             complement(52638..53156)
                     /locus_tag="ACG8Z3_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822914.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="ACG8Z3_000567"
     CDS             complement(53172..53498)
                     /locus_tag="ACG8Z3_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0822915.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="ACG8Z3_000568"
     CDS             complement(53511..55490)
                     /locus_tag="ACG8Z3_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0822916.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVARRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="ACG8Z3_000569"
     CDS             complement(55511..55825)
                     /locus_tag="ACG8Z3_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0822917.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="ACG8Z3_000570"
     CDS             55993..56286
                     /locus_tag="ACG8Z3_000570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0822918.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="ACG8Z3_000571"
     CDS             complement(56364..57476)
                     /locus_tag="ACG8Z3_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0822919.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57629..58477
                     /locus_tag="ACG8Z3_000572"
                     /pseudo
     CDS             57629..58477
                     /locus_tag="ACG8Z3_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(58830..>59221)
                     /locus_tag="ACG8Z3_000573"
     CDS             complement(58830..>59221)
                     /locus_tag="ACG8Z3_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0822920.1"
                     /translation="LQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQ
                     APTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGET
                     IISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17746 a        12065 c        10347 g        19063 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aatttattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgccgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 a
//
LOCUS       DAZKSU010000009        55197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000009 DAZKSU010000000
VERSION     DAZKSU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.009"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            368..1471
                     /locus_tag="ACG8Z3_000574"
     CDS             368..1471
                     /locus_tag="ACG8Z3_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0822921.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1755..2435
                     /locus_tag="ACG8Z3_000575"
     CDS             1755..2435
                     /locus_tag="ACG8Z3_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0822922.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2525..3559
                     /locus_tag="ACG8Z3_000576"
     CDS             2525..3559
                     /locus_tag="ACG8Z3_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0822923.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3573..3908
                     /locus_tag="ACG8Z3_000577"
     CDS             3573..3908
                     /locus_tag="ACG8Z3_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822924.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3892..6372
                     /locus_tag="ACG8Z3_000578"
     CDS             3892..6372
                     /locus_tag="ACG8Z3_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822925.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6428..7987
                     /locus_tag="ACG8Z3_000579"
     CDS             6428..7987
                     /locus_tag="ACG8Z3_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0822926.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7984..9348
                     /locus_tag="ACG8Z3_000580"
     CDS             7984..9348
                     /locus_tag="ACG8Z3_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0822927.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLTRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9519..10589
                     /locus_tag="ACG8Z3_000581"
     CDS             9519..10589
                     /locus_tag="ACG8Z3_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822928.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10604..11272
                     /locus_tag="ACG8Z3_000582"
     CDS             10604..11272
                     /locus_tag="ACG8Z3_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822929.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11292..11870
                     /locus_tag="ACG8Z3_000583"
     CDS             11292..11870
                     /locus_tag="ACG8Z3_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0822930.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12273..13184
                     /locus_tag="ACG8Z3_000584"
     CDS             12273..13184
                     /locus_tag="ACG8Z3_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0822931.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13440..14357)
                     /gene="rnhC"
                     /locus_tag="ACG8Z3_000585"
     CDS             complement(13440..14357)
                     /gene="rnhC"
                     /locus_tag="ACG8Z3_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0822932.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14559..14996
                     /gene="zapA"
                     /locus_tag="ACG8Z3_000586"
     CDS             14559..14996
                     /gene="zapA"
                     /locus_tag="ACG8Z3_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0822933.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15054..15602
                     /locus_tag="ACG8Z3_000587"
     CDS             15054..15602
                     /locus_tag="ACG8Z3_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0822934.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15717..18077
                     /locus_tag="ACG8Z3_000588"
     CDS             15717..18077
                     /locus_tag="ACG8Z3_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0822935.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18164..18478
                     /gene="trxA"
                     /locus_tag="ACG8Z3_000589"
     CDS             18164..18478
                     /gene="trxA"
                     /locus_tag="ACG8Z3_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0822936.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18660..20438
                     /gene="uvrC"
                     /locus_tag="ACG8Z3_000590"
     CDS             18660..20438
                     /gene="uvrC"
                     /locus_tag="ACG8Z3_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0822937.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20583..21482
                     /locus_tag="ACG8Z3_000591"
     CDS             20583..21482
                     /locus_tag="ACG8Z3_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822938.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21475..22737
                     /locus_tag="ACG8Z3_000592"
     CDS             21475..22737
                     /locus_tag="ACG8Z3_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0822939.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22900..24798
                     /locus_tag="ACG8Z3_000593"
     CDS             22900..24798
                     /locus_tag="ACG8Z3_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HFQ0822940.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGQTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25097..25816)
                     /locus_tag="ACG8Z3_000594"
     CDS             complement(25097..25816)
                     /locus_tag="ACG8Z3_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0822941.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    26006..26053
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            26153..26485
                     /locus_tag="ACG8Z3_000595"
     CDS             26153..26485
                     /locus_tag="ACG8Z3_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822942.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26472..28475
                     /locus_tag="ACG8Z3_000596"
     CDS             26472..28475
                     /locus_tag="ACG8Z3_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0822943.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28494..28964
                     /locus_tag="ACG8Z3_000597"
     CDS             28494..28964
                     /locus_tag="ACG8Z3_000597"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0822944.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28985..29569
                     /locus_tag="ACG8Z3_000598"
     CDS             28985..29569
                     /locus_tag="ACG8Z3_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFQ0822945.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29584..30570
                     /locus_tag="ACG8Z3_000599"
     CDS             29584..30570
                     /locus_tag="ACG8Z3_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0822946.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30560..30871
                     /locus_tag="ACG8Z3_000600"
     CDS             30560..30871
                     /locus_tag="ACG8Z3_000600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0822947.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30893..32674
                     /locus_tag="ACG8Z3_000601"
     CDS             30893..32674
                     /locus_tag="ACG8Z3_000601"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0822948.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMCG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32667..34043
                     /locus_tag="ACG8Z3_000602"
     CDS             32667..34043
                     /locus_tag="ACG8Z3_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0822949.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34050..34685
                     /locus_tag="ACG8Z3_000603"
     CDS             34050..34685
                     /locus_tag="ACG8Z3_000603"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0822950.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34715..36070
                     /locus_tag="ACG8Z3_000604"
     CDS             34715..36070
                     /locus_tag="ACG8Z3_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0822951.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36262..37656)
                     /locus_tag="ACG8Z3_000605"
     CDS             complement(36262..37656)
                     /locus_tag="ACG8Z3_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0822952.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37748..38719
                     /locus_tag="ACG8Z3_000606"
     CDS             37748..38719
                     /locus_tag="ACG8Z3_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0822953.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38716..39498
                     /locus_tag="ACG8Z3_000607"
     CDS             38716..39498
                     /locus_tag="ACG8Z3_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0822954.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39667..40038)
                     /locus_tag="ACG8Z3_000608"
     CDS             complement(39667..40038)
                     /locus_tag="ACG8Z3_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0822955.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40172..40615
                     /locus_tag="ACG8Z3_000609"
     CDS             40172..40615
                     /locus_tag="ACG8Z3_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0822956.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40658..41467)
                     /gene="yidA"
                     /locus_tag="ACG8Z3_000610"
     CDS             complement(40658..41467)
                     /gene="yidA"
                     /locus_tag="ACG8Z3_000610"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0822957.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41486..42853)
                     /locus_tag="ACG8Z3_000611"
     CDS             complement(41486..42853)
                     /locus_tag="ACG8Z3_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0822958.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42837..43667)
                     /locus_tag="ACG8Z3_000612"
     CDS             complement(42837..43667)
                     /locus_tag="ACG8Z3_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0822959.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43830..44267
                     /locus_tag="ACG8Z3_000613"
     CDS             43830..44267
                     /locus_tag="ACG8Z3_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0822960.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44343..44963
                     /gene="rpoE"
                     /locus_tag="ACG8Z3_000614"
     CDS             44343..44963
                     /gene="rpoE"
                     /locus_tag="ACG8Z3_000614"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0822961.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45152..45658
                     /locus_tag="ACG8Z3_000615"
     CDS             45152..45658
                     /locus_tag="ACG8Z3_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0822962.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45675..46574
                     /locus_tag="ACG8Z3_000616"
     CDS             45675..46574
                     /locus_tag="ACG8Z3_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0822963.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46661..47545
                     /locus_tag="ACG8Z3_000617"
     CDS             46661..47545
                     /locus_tag="ACG8Z3_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0822964.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47737..47808)
                     /locus_tag="ACG8Z3_000618"
     tRNA            complement(47737..47808)
                     /locus_tag="ACG8Z3_000618"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47773..47775),aa:Arg,seq:ccg)
     gene            47868..49202
                     /gene="rpoN"
                     /locus_tag="ACG8Z3_000619"
     CDS             47868..49202
                     /gene="rpoN"
                     /locus_tag="ACG8Z3_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0822965.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49351..50442
                     /locus_tag="ACG8Z3_000620"
     CDS             49351..50442
                     /locus_tag="ACG8Z3_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0822966.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            50483..51484
                     /gene="gap"
                     /locus_tag="ACG8Z3_000621"
     CDS             50483..51484
                     /gene="gap"
                     /locus_tag="ACG8Z3_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0822967.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51594..52784
                     /gene="pgk"
                     /locus_tag="ACG8Z3_000622"
     CDS             51594..52784
                     /gene="pgk"
                     /locus_tag="ACG8Z3_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0822968.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52888..53643
                     /gene="tpiA"
                     /locus_tag="ACG8Z3_000623"
     CDS             52888..53643
                     /gene="tpiA"
                     /locus_tag="ACG8Z3_000623"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0822969.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53759..55057
                     /gene="eno"
                     /locus_tag="ACG8Z3_000624"
     CDS             53759..55057
                     /gene="eno"
                     /locus_tag="ACG8Z3_000624"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0822970.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        18304 a         9607 c        11687 g        15599 t
ORIGIN      
        1 ataccgaggg tcagctgatt gaacaccgtt ttatcgagag gttgtgaaaa agtcgcttag
       61 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca taaatttgac
      121 ttaataccga gaaaatggct tttgaacacc gtttattcaa aaaaagggac tttgacacta
      181 cttttgtcaa agtcccatcg gttttttatc tatttttatt acaatcattt cttccagagt
      241 agtgacatct ggaatggctg aaatgtaaac agattatttg cctgtatcaa tgtcgaagag
      301 taatataaaa agtgtaaacg ctatcatatt gttcatcatt caaattttat agagagaagg
      361 cattgagatg gaaaaaacat atcaagcagg tacaaatgaa ggaatagtag attttatcaa
      421 tatggaagat ttagaaatcg cggcttcaca agtgattcca gctggtggtt atggttacat
      481 cagcagtggt gctggcgact tatttactta tcaagaaaat gaacgggcat tcaatcatcg
      541 tttgattatt cctcatgtat taagagatgt agaattaccc gatacaacta cacattttga
      601 cgaagaaatg ctgactgcac caatcatcat ggcaccagtt gctgctcatg gattagctca
      661 tgtgaaggcg gagaaagctt ctgcaaaagg tgtagcagat tttgggacga tctatacagc
      721 aagttcttac gcttcttgta ctttagaaga gataagagaa gcaggcgggg agaaagcacc
      781 gcaatggttt caattttata tgagcaaaga taatggaatc aatttggata ttcttgaagt
      841 ggccaagcgt aatggagcaa aagcaatcgt attgacagcg gatgcaacag ttggcggaaa
      901 ccgtgaaaca gaccgcagaa acgggtttac cttcccgctg ccaatgccga tcgttcaggc
      961 ttatcaatca ggagtaggcc aaacgatgga tgctgtatat aaatcttcca aacaaaaact
     1021 tagtccaaaa gatgtagaat ttatcgctgc tcattctgat ttgccagtct acgtgaaagg
     1081 tgttcaatca gaagaagatg tctatcgatc attagaatct ggtgctggtg gaatttgggt
     1141 atctaaccat ggtggccgtc aattagatgg ggggcctgct gcgtttgatt cgttgcaata
     1201 tgtggcagaa gcagtagata aacgtgtacc aatcgtgttt gacagcggtg tacgacgtgg
     1261 acaacatgtt ttcaaagcaa tcgcatcagg tgccgatctt gttgctattg gcagacccgt
     1321 tatttatgga ttgtctttag gaggaagtac aggagtccat caagtgtttg atttctttaa
     1381 aacagaattg gaaatggtca tgcaattagc tggaacacaa acagtagaag atatcaaaaa
     1441 aataaaattg cgtgagaatc gttttattta agaaataaat gacgaaaaaa ataggagtgc
     1501 ggcagatgat gccgtactcc tatttttata gttcaaggat gtcactaata gaataatttg
     1561 aaacgaaaaa ataaaattga aatatttttt catacttaat aaaaaaggaa gaatattcat
     1621 gtaaacgatt ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc
     1681 ggatgaattc atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa
     1741 aggagtgatt tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc
     1801 taggcggctt ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta
     1861 cctttactga accaacaaat accgttgagc ctcttaaccc tactgatcct tcaaaaccag
     1921 tagagccagc agatccggaa aatccagcaa ctggccagac aggttcttta acattggatg
     1981 tagttcctga attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag
     2041 tagacgcttc aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg
     2101 cagatggcca agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt
     2161 cacaagtttt gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag
     2221 ataacgaatc cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag
     2281 cgacaccaat atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg
     2341 atccagcaaa tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt
     2401 tgacttggaa cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg
     2461 cggtgaaaat actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg
     2521 gacaatgaat caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt
     2581 catttttccg tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt
     2641 atttccagag aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga
     2701 acaaaatcag acactagaag taattatcag caattcttca gatgaagaac gaacatttga
     2761 ggtctctgtc aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa
     2821 cccaacgtta gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa
     2881 ggaaatcaat gtatcagcac atgcagaaac aactatacct atagaagtga aaatcccagc
     2941 aaaaagcttt aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga
     3001 aacagaaaat gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt
     3061 cgttttacaa gaaagtcaag agacgataga accggatctg aaacttttat caggagattt
     3121 ggacgaggtc aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat
     3181 tatcagcaat ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa
     3241 tacgtctaat gaatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc
     3301 tggtgataaa gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag
     3361 taatgaatgg cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa
     3421 tgaaaattcc gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt
     3481 tgctgcagtt ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa
     3541 aaaaggagaa acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga
     3601 ttattgcaag tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt
     3661 caggtacgat tacattttat gatctaagtc cagttccgca tccaagtggt tcagtcaatg
     3721 gcgcatcaaa acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat
     3781 tcccggagac gaatgaagaa aagatccgtt ttctttattt gatcattggt atagaattat
     3841 ggcttcttgc gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta
     3901 caaaataaat tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata
     3961 tttatctttt caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat
     4021 ccatacacgt atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg
     4081 aagcttgtcc aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac
     4141 gatggttcaa atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg
     4201 aaactaacaa atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg
     4261 atcatcaatt tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac
     4321 aacgcctact tgtatattgc cggtgccccc atcaaggtta ctagcggtag tttattatca
     4381 gcaggaagcc ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattggca
     4441 gatattattg ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt
     4501 gatgttacag ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca
     4561 aacaaaattt tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct
     4621 gttgattttt atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct
     4681 ccagctgcag gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat
     4741 gcattttctg caagtgcaga gataaatacg tggaatagtt acatctcaac aagtgattca
     4801 acaggaaaat ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac
     4861 cttccaggct ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat
     4921 cagttgccat ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca
     4981 caaggagcaa tactgactca tactccagca agcacaagtg gattgaatca gacagccaat
     5041 atcgtgtaca gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta
     5101 ttggagcagt acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag
     5161 tggggaaagc tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa
     5221 ctaaaaaagg gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca
     5281 ggatggacag atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct
     5341 acttctacac tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac
     5401 gatgtactta ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga
     5461 atcaccactc ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag
     5521 ctaaaaaacc ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat
     5581 acgattcaag aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa
     5641 aaaatcacga tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat
     5701 ggtacaacta tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg
     5761 agcaaaatag gagataccat tcatcttact tacaactatc aattaaatac tgtagatact
     5821 tctgtacaat ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta
     5881 gcagatggtg ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc
     5941 cccaaacaag aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc
     6001 cccaaaccgt tgaagacaag ttattatgaa gcaaaaggcg atttttcttt tgatgtgcgg
     6061 gatactcggc ttccaagcac gtctccttgg caactgacag ggacattgac ttctttattt
     6121 aagaatgatc aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca
     6181 aaacaattga ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa
     6241 ggagaggttc tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt
     6301 acgaatgctc aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg
     6361 ccaaccagct aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt
     6421 ttcaaggatg agaaggaaac ttctgctgtt aaaatcagaa atgcttggag gagtggactt
     6481 ggaacttttc agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt
     6541 attagaggaa aaaggagaga tttccgttaa taacgtactt acccgcttat cagttgatcg
     6601 ggctaccttg aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt
     6661 ggaaattgtt atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt
     6721 cgatgtttat tatcattgct acttgcgcta ttctacaaaa tatcagatac ttctttatct
     6781 gttggaaaat ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc
     6841 tactttgtct cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat
     6901 aaaaagcggg cggctgaccg gtccagaaag tcaaatttgt tatttttatt tccaattatt
     6961 ttggtttgga cgtccttatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac
     7021 ttttttagat ctgctgcaaa aagagtttag ttttactatt acagaagaag gacgattgaa
     7081 atttgaaata tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc
     7141 agattatagc aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca
     7201 tacatttttg acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt
     7261 ggttttttat cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca
     7321 ggagtttttg ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt
     7381 taaacaatcg atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa
     7441 gattgcgttg acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa
     7501 tatttttggc caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct
     7561 ggttttatgt gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga
     7621 cgaggaaaat gcccgctttg aattatttgc tcgctatgca tctattttgc ggacgctgat
     7681 caagcagtta gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc
     7741 ggatatcgtg aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt
     7801 cgaatacgag aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc
     7861 gtcacagaag tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc
     7921 ctatttgaac caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa
     7981 aacatgaaaa tcgtgatcat tggtgcttcc catgcaggca tcacagctgc cctaaatcta
     8041 agaaaacttc aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta
     8101 ggctatgttt caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa
     8161 gttgcccata acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga
     8221 gtaactgaac ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac
     8281 gagaccataa cctatgataa attaatcgta gcaacaggtt cctctcctgt caccttgtat
     8341 aaacaaattg aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta
     8401 cttgccgctt taaaagaagc aaaagaagta gtgatcttcg gtgctggtta tattggattg
     8461 gaattagcag atgctttgag aaataaagga tacattattc atctagttga ttatatgccg
     8521 aatgttttaa caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa
     8581 actaagcaaa tcaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag
     8641 aaagtagtat ccgtacaact tctatctcaa gcaataaagg cagatatggt cattttttct
     8701 gcccaaactc gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag
     8761 actgtgatag tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat
     8821 atcgtcccag tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga
     8881 gctgtacata tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac
     8941 ctcagacaaa agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg
     9001 acagaagatg aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat
     9061 ctatttccac aatacgatga agaaaacaaa acagtaaatg caaagctgat ctaccatccg
     9121 gatacattag aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg
     9181 aacctgcttg cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat
     9241 tttggatttc tgtctgaaca cacctctcgt tttcactacc tcaatgagtt agcttttaaa
     9301 gtactagcta agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg
     9361 tcttttattg tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa
     9421 tgaaatagcg gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa
     9481 ttgttgtata aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa
     9541 ttatgcattc aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc
     9601 tggtattttt tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg
     9661 acaagtggga ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt
     9721 ccatgattac taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc
     9781 agacacctgg agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct
     9841 tcttcggtat cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg
     9901 ataacgatga tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccgtag
     9961 gagatacagt taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc
    10021 atgccaaatt caatgtctca ccagttctct atacaaccat caatgcttac caagagatcc
    10081 gcttcgaaaa agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta
    10141 aaatcagtaa tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat
    10201 tacctggata taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta
    10261 ttgcggctat tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct
    10321 ttggtgtaat gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac
    10381 aaacattcat tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat
    10441 cccttgttct acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta
    10501 gcttgttgat gctggtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa
    10561 aaatcgatcc gttgaaggca attggataag aaggaggaag agcatgaaag caatagaatt
    10621 taaagaagta gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac
    10681 aaccatgagt atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa
    10741 aagtactttt ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat
    10801 caatgatcaa gaattcagta atcaaaaaga aagtgaacgc gttaaactac gttttaaaga
    10861 aattggcttt attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt
    10921 attattagta gataagatca aacatgaaca tcgtcaaaaa gaagcaaaag aattatttga
    10981 gcagcttggc gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg
    11041 gcaaagggtc gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga
    11101 accaactgct agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga
    11161 atcaaaagac aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa
    11221 atgcgataga atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag
    11281 caggtggaaa aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag
    11341 cgactgactt gtttatgtcc aagggatatc tcggcacttc aacaagagag atcgcacaaa
    11401 aagttgggat cacccaaccc aatctctatc attatttcgg tgataaagaa aaactatata
    11461 cggcagtttt agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa
    11521 caagcgaaac gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac
    11581 atctggtcga tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa
    11641 gagatcatct tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt
    11701 cagcaaacca atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt
    11761 tcttagtgtt agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg
    11821 tggcgcaatt gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata
    11881 tagggagaaa aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgttt
    11941 tttttatata ggtagttttt cataaataca aatttgttat tctctcatga ttatcagggt
    12001 atatttttat ttattatgtc ggtgttccag gaagtgtttt aatttaataa cgaagaaatt
    12061 aattgtttgt agaatgaagg aaactattta ttgggtagat gaatcaccac gaatagtcaa
    12121 aaggagattt caggatatgc caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc
    12181 tacggttata tcttttacag cccctttttt gcgtacttaa gctgatttta agttttgacc
    12241 cttagtctgg atctacgagg tgagagtcaa gaatgaaaaa aataattgga gcttttgtat
    12301 ttgcaatcat tatcattatt atctgttttc aaatagatgt ttttggtcaa aaaaagatag
    12361 ttggacaaac ggtcagcagt acgaacagta cggttacaaa gacaaaggaa tcatttgatg
    12421 atacagaaca agagacatta atcaaaatcg gaaaaaatga ccgtacacta tatacagatg
    12481 actcgctaac taccccactt gcaactataa acggcggaga gctggcggag tttctttctg
    12541 aaacaaaaaa tgcttatcag atcaagacaa atgatggcta tactggatat ttagccctcg
    12601 aagatggaac caaagtcaca aagaatatac aaacaaaacc ggaagagcta tctgatgcag
    12661 tgattgtatt agatcctggg catggcggaa atgatactgg ggcattaagc aatgatgaac
    12721 aaacagaaga aaaagatata acattaagta cagccataaa agtaaaaaag gcgctagagg
    12781 atgctggagc aacggtttat ttgacccata ctacagatga attagtacag cttggagata
    12841 tatgcgacta cagtgaagaa aaaaaggcag atgtctttat cagtttacac gcagactcta
    12901 cggaatatgc gaatgaagca actggaatca cgacatatta ttactatgga caagaggaaa
    12961 cattagcaca gacaattgct gactccttca ctgatctgcc tctggattca agaggaatct
    13021 ccactgggaa ctatcaagtt cttcgagaaa atcttcaacc ttctatttta atcgagatgg
    13081 gttatatgaa taatgatagt gatttaaagg aattagtaac agacagttat cagcagcaga
    13141 ttgctgcaag tttgacacaa gcattgacca cttattttca gtaataaata agcccagcca
    13201 aacaaaaata gctcctcata tttttcgttt ggccgggctt attatcgcag atgcaagctt
    13261 ttgaacaccg tttaaggaag aggttgtgag cctgccgttt aactccgcgt cacaaggagc
    13321 aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg
    13381 catgagaaca ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat
    13441 tattttttta gcagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt
    13501 tcaagcatag gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt
    13561 ccagctcctg atggaagggt gattccagct tcagctgatg ctttgtgtaa ctcttccaaa
    13621 aagctggcac gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta
    13681 gtaacgaagt acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa
    13741 tttgcttccg gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt
    13801 tttagcagca aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat
    13861 tttggttgga tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt
    13921 ttttttaaga tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta
    13981 acaccaagtt tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt
    14041 agcggaccaa aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg
    14101 atattttttg gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc
    14161 ccccatttat cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta
    14221 taagcggtaa tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta
    14281 tttaacaaat aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg
    14341 ataatgacgt tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca
    14401 cgacttttcg ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa
    14461 tagttaagta tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac
    14521 taaataaaac tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct
    14581 acaaagcagt gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata
    14641 tggatatcgt aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac
    14701 aaatggataa tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga
    14761 agaaacaaga aagaatcttg gagctggaag aagaaacagc agaattaaag aaaaaaatga
    14821 tcaaatttac agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc
    14881 gggaagtatt gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac
    14941 aacagatttt aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc
    15001 cttttatcaa aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga
    15061 gtttgctgat tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg
    15121 ctttgcaaat catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt
    15181 acaaaccatt agcaagtcat ttggaattat acattccata cccagctgtc accccaacat
    15241 caaagttagc attttttgat caggtatttg cttttcacct agatgaagct ttttatgcag
    15301 gaactgcttt tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt
    15361 ttgtccatgg attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca
    15421 tcctgtcact aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg
    15481 ttccattaga tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg
    15541 agagaacgcc atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt
    15601 aaggaaaata gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc
    15661 tgcgtagcgt atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga
    15721 ataaccgaat ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg
    15781 tagtgactgc acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa
    15841 cgatcacaaa ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg
    15901 ggatccctat acctaaactt gaaaatatcc gtccccatat gaaacggatc gagatcggag
    15961 cagacttgaa tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat
    16021 tgaatcgttt cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt
    16081 gggcagatca gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg
    16141 aagacggacg cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc
    16201 ggcgaagtga acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa
    16261 agtatttaag tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac
    16321 aagaataccg cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt
    16381 tatttattga accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg
    16441 ctgaacgaaa tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc
    16501 gtcaagaaat catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag
    16561 cacgatttgg taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg
    16621 tcttgaaaca agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca
    16681 caatcggaaa agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga
    16741 tcacgttgaa gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg
    16801 caggtgaaga aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac
    16861 agtcgattga gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt
    16921 tatcaaaagt caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc
    16981 cgcaagaagg tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt
    17041 atgtgatggc aacaacgcat tatcctgaat tgaaagttta tggatacaat cgggcaaata
    17101 cgattaatgc cagcatggag ttcgatgtgg atacactaag tccaacgtat cgtttgctga
    17161 ttggcgttcc tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag
    17221 aagttattga tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga
    17281 tcacagattt agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt
    17341 ttgtttctga ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg
    17401 aagaacgtga aaaagaaatg gaaaaagcaa agaaaaaagc aaacgaagtc gtgtcagagg
    17461 cggaagaaaa agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc
    17521 aaggaaatgt gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc
    17581 aagaagaaac tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga
    17641 aaccgggaga cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa
    17701 acgggaatca gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc
    17761 tgacgcctgt tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg
    17821 aatctagtag tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc
    17881 taagtgaggt agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta
    17941 ttgtccacgg aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc
    18001 atcggagcgt caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa
    18061 ttgttaaatt taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga
    18121 aatgctatac taatcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac
    18181 agataagaca tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac
    18241 ttggtgtggt ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga
    18301 tgaagaagaa ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca
    18361 attcggtatc atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa
    18421 agctgttggt gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa
    18481 acttacagca aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg
    18541 cggttgagtt aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg
    18601 attgtttttt tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga
    18661 tgaacgaacg aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga
    18721 tgaaagataa aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg
    18781 tccgctcata ttttactggc agccataata cgaaaacgga acggcttgtt agtgaaatcg
    18841 aagactttga atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc
    18901 tgataaagaa aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt
    18961 tccttaagat aacgaatgaa aaatatcccc gtttggtgat tactcgtaaa gtactaaaag
    19021 acaaagcact gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga
    19081 tcctcgatcg attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc
    19141 tttactatca tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct
    19201 atacgaaaat cgttgatgaa gtgaaaaagt ttttaaacgg aggacacgaa aaaatcgagt
    19261 cagagatcca tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag
    19321 aataccgaga ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata
    19381 ctgatttact ggatcgagat gtatttggtt atgcgattga caaaggatgg atgtgtgtcc
    19441 aagtgttttt cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct
    19501 ataacgaagc agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc
    19561 atttcattcc taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt
    19621 tgttagctac gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg
    19681 cgaataagaa tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg
    19741 aacggacgat cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac
    19801 gtatcgagtc ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg
    19861 tattcattga tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca
    19921 acggaccgga tgattatgca tcaatgagag aagtcatcta tcgcagatat tcacgagtga
    19981 ttcgcgaagg gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag
    20041 atgtggcaaa agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg
    20101 caaaaaacga taaacataaa acaaatgagt tgctattcgg tatagatttg gctgtagtac
    20161 cgcttgagcg aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc
    20221 gattcgcaat cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag
    20281 atgaaattga agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt
    20341 tgaaaaatat cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg
    20401 tcgcacaaaa cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa
    20461 agtaaagtgt cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat
    20521 aataaatcgc ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag
    20581 aaatgttaga agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg
    20641 tctctttcca aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga
    20701 agacaacgac ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat
    20761 ggaatggcca tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac
    20821 ggggacttta tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag
    20881 ggaaatcaag aaaagagatt gaaccaaaaa tcgatttttg gatggaaaaa tttcaggtaa
    20941 aagggaaaaa gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc
    21001 tgatcgccac gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt
    21061 tagatccggt aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt
    21121 catgtgtcat tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga
    21181 tcatgttgcg aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat
    21241 ttggccgtac gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg
    21301 aaggcgtgaa aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc
    21361 ctgaagctgg gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta
    21421 accagcaacc gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt
    21481 aagttttgga ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg
    21541 atcatgatat tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct
    21601 caacaaaata gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag
    21661 caaatgagcc aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa
    21721 gcacaaaaag agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat
    21781 gataagataa atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg
    21841 atacaacaac aactgactgg cttgcaatcg atgcttcgag caagtcaatt aggcatttca
    21901 ccagaagaag tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt
    21961 gattcaaaag gaaaaatgac agttggagaa gataactcga cagtccaata tgtcgtaagt
    22021 tatgtagcaa cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa
    22081 gagatcgctt ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca
    22141 caaaagcatt tttacggaaa actaacaggt gttttacttg ttgcattaac gcaaatggca
    22201 ttatacggta ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa
    22261 aatatattag acggtatctc attggataac ttgattggac cattcttgtg gtattctctt
    22321 ctattcatga tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg
    22381 gtcaacaaag cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc
    22441 ggatatatgc ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc
    22501 acttcgtata ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca
    22561 gtcggtatgg gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg
    22621 atgatcttgt ctgcaaacat gtacaaatcg aatgtactcg tgtatagcga aggcggatta
    22681 tggagttcat tgaaacaatc gatttcaatt atgagaaatg aacgaaaaaa agcataaaat
    22741 atagaaaaat atagaaggaa gctggacatt tattgttccc agcttctttt ttgtatagac
    22801 ctatttctat ttttttatta tattggatat atatattcat tgaaaataga gcataacaac
    22861 tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta tttagattta
    22921 ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa tctcgaagca
    22981 attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg cgaatttcct
    23041 gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt
    23101 ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac ggcttatcca
    23161 gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct gatccatcgt
    23221 ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt tcgaaaagga
    23281 ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga tcgtatcaac
    23341 gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa
    23401 aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca tttgcatatc
    23461 gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg aatcatggat
    23521 ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg tgcgtattgc
    23581 ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa ctatttattt
    23641 gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc ccatcaaacc
    23701 tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga aagagagcaa
    23761 gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc agaacatcag
    23821 ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga aaaaatcaac
    23881 tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg gacttgtttt
    23941 caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat
    24001 agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac gtttttgatc
    24061 gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg ctgtatacct
    24121 gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg cggaaaagaa
    24181 ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac tgcaaatgaa
    24241 gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc tttattcggc
    24301 aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac gcacgtcgaa
    24361 aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa aattttggca
    24421 gaattcgatg caaaagaaga aggttcagca atcatcaatg gacagacgcc agtaaaagta
    24481 aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga cggaggtcta
    24541 tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg caattcttac
    24601 ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa
    24661 tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg
    24721 aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag gctgttggat
    24781 tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact aaaaagcgac
    24841 tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg taaaatatta
    24901 gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt tcacgacctc
    24961 ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca
    25021 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
    25081 ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc tgttcagatg
    25141 aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg tgcatattat
    25201 ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct tttaacaaag
    25261 gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa tcagggagaa
    25321 ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac tgcggggcag
    25381 tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct tgataaagtg
    25441 ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa atcggttttc
    25501 tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga atatttgttt
    25561 ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc tgaccgcctg
    25621 caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga gacatgactg
    25681 gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc tgcggtgtat
    25741 agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc ccggcaatgc
    25801 cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa ggatttaaaa
    25861 aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
    25921 taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
    25981 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
    26041 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
    26101 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
    26161 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg
    26221 aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat tggagataat
    26281 ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa
    26341 gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
    26401 tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt
    26461 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
    26521 atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt
    26581 atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc acgattattg
    26641 ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa gaagctatcc
    26701 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
    26761 tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat aaattagaag
    26821 aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat cgtttagaag
    26881 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
    26941 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
    27001 aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
    27061 tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa attgctggga
    27121 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag
    27181 aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag
    27241 gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
    27301 cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag
    27361 gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag
    27421 aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc
    27481 ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca
    27541 gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct
    27601 tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac
    27661 tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga
    27721 ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat
    27781 taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg
    27841 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat
    27901 tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt
    27961 ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa
    28021 aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct
    28081 taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata
    28141 atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac
    28201 taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc
    28261 cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
    28321 ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg
    28381 gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg
    28441 tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg
    28501 attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga
    28561 cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
    28621 cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag
    28681 gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg
    28741 atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag
    28801 gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga
    28861 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga
    28921 ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa
    28981 gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag
    29041 agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
    29101 gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa
    29161 ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa
    29221 gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga
    29281 atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt
    29341 aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg
    29401 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
    29461 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
    29521 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
    29581 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
    29641 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
    29701 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
    29761 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
    29821 atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc
    29881 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
    29941 accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac
    30001 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
    30061 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
    30121 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
    30181 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
    30241 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
    30301 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat
    30361 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
    30421 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
    30481 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga
    30541 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
    30601 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
    30661 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
    30721 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
    30781 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
    30841 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
    30901 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
    30961 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgtg
    31021 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
    31081 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
    31141 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
    31201 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
    31261 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
    31321 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
    31381 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
    31441 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
    31501 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
    31561 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
    31621 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
    31681 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
    31741 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
    31801 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
    31861 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
    31921 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
    31981 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
    32041 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
    32101 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
    32161 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
    32221 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
    32281 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
    32341 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
    32401 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
    32461 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
    32521 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
    32581 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
    32641 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
    32701 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
    32761 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
    32821 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    32881 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    32941 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    33001 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    33061 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    33121 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    33181 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    33241 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    33301 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    33361 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    33421 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    33481 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    33541 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    33601 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    33661 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    33721 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    33781 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    33841 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    33901 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    33961 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    34021 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    34081 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    34141 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    34201 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    34261 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    34321 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    34381 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    34441 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    34501 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    34561 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    34621 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    34681 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    34741 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    34801 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    34861 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    34921 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    34981 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    35041 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    35101 catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc
    35161 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    35221 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    35281 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    35341 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    35401 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    35461 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    35521 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    35581 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    35641 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    35701 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    35761 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    35821 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    35881 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    35941 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    36001 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    36061 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    36121 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    36181 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    36241 attcctttat ttatcaatag gttataattc atattttttt attttttctt ttacgatact
    36301 tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc
    36361 tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa
    36421 gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg
    36481 gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt
    36541 tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt
    36601 gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat
    36661 cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa
    36721 ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat
    36781 gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag
    36841 accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc
    36901 tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc
    36961 ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa
    37021 atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct
    37081 atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac
    37141 aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt
    37201 agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg
    37261 gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact
    37321 aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc
    37381 aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc
    37441 aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag
    37501 ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc
    37561 taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca
    37621 agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat
    37681 accatacaat tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga
    37741 attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc
    37801 gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc
    37861 tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc
    37921 ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca
    37981 tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact
    38041 acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc
    38101 gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg
    38161 tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt
    38221 tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta
    38281 tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat
    38341 catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga
    38401 cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt
    38461 gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga
    38521 cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg
    38581 cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga
    38641 cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga
    38701 gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg
    38761 ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg
    38821 caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa
    38881 gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca
    38941 ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt
    39001 tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta catggatttt
    39061 cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct
    39121 aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg
    39181 gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc
    39241 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    39301 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    39361 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    39421 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    39481 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    39541 accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    39601 ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    39661 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    39721 agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccgg cagctttatg
    39781 ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc
    39841 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catccccagg
    39901 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    39961 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca
    40021 tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa
    40081 gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    40141 gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa
    40201 gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca
    40261 gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg
    40321 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    40381 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    40441 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    40501 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    40561 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    40621 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    40681 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    40741 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    40801 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    40861 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    40921 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    40981 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    41041 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    41101 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    41161 cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga
    41221 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    41281 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    41341 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    41401 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    41461 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    41521 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat
    41581 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    41641 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    41701 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag
    41761 aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt
    41821 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    41881 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    41941 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    42001 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    42061 actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat
    42121 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    42181 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    42241 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    42301 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    42361 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    42421 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    42481 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    42541 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    42601 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag
    42661 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    42721 gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat
    42781 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    42841 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    42901 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    42961 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    43021 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    43081 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg
    43141 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    43201 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    43261 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    43321 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg
    43381 aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt
    43441 ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga
    43501 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    43561 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    43621 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    43681 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    43741 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    43801 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    43861 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    43921 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    43981 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    44041 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    44101 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    44161 gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg ttcatgggac
    44221 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa
    44281 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    44341 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    44401 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    44461 tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    44521 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    44581 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    44641 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    44701 acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg
    44761 atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact
    44821 tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg
    44881 aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat
    44941 tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat
    45001 cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata
    45061 aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg
    45121 tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg
    45181 gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat
    45241 gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga
    45301 tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc attttagtgc
    45361 atggttttat cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac
    45421 gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt
    45481 tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct
    45541 ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata tgaatgaaaa
    45601 cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag
    45661 gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata
    45721 aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag
    45781 agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc ggagtatctt
    45841 tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg
    45901 cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg
    45961 atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa
    46021 ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa
    46081 aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc
    46141 agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag
    46201 cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct
    46261 cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa gaagtcaaac
    46321 ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata
    46381 ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga
    46441 acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg
    46501 gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt
    46561 atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca
    46621 agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt
    46681 tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt
    46741 gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca
    46801 atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat
    46861 tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag acagtacaaa
    46921 aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc
    46981 gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt
    47041 cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg
    47101 gcacgagatt ggcaatcaca catacgatca tcagaatctg gccactcttt ctgcccagtc
    47161 gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg gcaaaacccc
    47221 cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga tcatccagcg
    47281 accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc ctgatttgat
    47341 tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc atgatatcta
    47401 tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc aagggtatcg
    47461 catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact attatggcag
    47521 aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata acacatttta
    47581 ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc tatacaggta
    47641 caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa aataaagaag
    47701 caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga atcgaacccc
    47761 cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa gcactgggat
    47821 aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg aaatttgagc
    47881 aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa cagttgcaac
    47941 agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt gagaatcaag
    48001 caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat tacataaaag
    48061 cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt cctgatacaa
    48121 aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga gatacttttt
    48181 tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt ttgacgatta
    48241 gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc gatgcgttga
    48301 ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa gaatgtctca
    48361 tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg ttggaagaat
    48421 gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc
    48481 tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc cctggccgga
    48541 ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta gtggacgacg
    48601 ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga
    48661 gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat ttaaaagaac
    48721 ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat acaattttgc
    48781 gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag gaacgtccaa
    48841 tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa tccacggtca
    48901 gtcgtgcagt caacgggaaa tatttggaaa cagacttcgg tgtatttgaa ttaaaaaaat
    48961 ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt tcagcagata
    49021 cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag ccgttgtctg
    49081 accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga cgaacagtag
    49141 caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt tatgataact
    49201 aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag ttgaaaaacg
    49261 agagcaaaac tgttagactt acagtcgtga ggttgagatt tccctttatt gatgtgaaat
    49321 tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac attggacgta
    49381 aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga atctgttgca
    49441 ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat ctattggatg
    49501 cagccgattg ggcgtcgaag tttatcggaa agtatgggga tcacagaacg agtcttacga
    49561 acagaaacag accttttgaa aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg
    49621 ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga tcagctttta
    49681 ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg ctgtatcgtt
    49741 gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga agcgctgagc
    49801 gaagccttag accgactact tccagaaggc gagaatatca tcgctgtgat gggaggaact
    49861 actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg gcataacctg
    49921 tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa ttctgtcagt
    49981 gcaagaatgg ctgctaaagc aaacggtaac caccgcgcac tttatgttcc ggaacaatta
    50041 agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt cttaaacttg
    50101 attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat ggcagcacgt
    50161 cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt tgcggaatct
    50221 tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag aatcggcata
    50281 gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa
    50341 gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct catcactgat
    50401 gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata tttttttgat
    50461 tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt ttggacgtat
    50521 tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag tagcaatcaa
    50581 cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg
    50641 tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa
    50701 agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg tagatatcgt
    50761 tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt taacagctgg
    50821 tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa tcgtttataa
    50881 cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt catgtactac
    50941 taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt
    51001 aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac ctcatcctaa
    51061 aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact caacaggtgc
    51121 tgctaaagct atcggtttag taattcccga attgaacggt aaattagacg gagctgctca
    51181 acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg aaaaagaagt
    51241 aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt acggatacaa
    51301 cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat tatttgatgc
    51361 tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg ttgcttggta
    51421 cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact tcgctaactt
    51481 ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa gcggggaagc
    51541 aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat aaaatggcta
    51601 agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt gttgacttca
    51661 acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca gcattgccaa
    51721 caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac cttggacgtg
    51781 taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg
    51841 aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa ttagaagaag
    51901 caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga tttgaagata
    51961 ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg gcttcattag
    52021 gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct tctaacgtag
    52081 gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa gaaatcaagt
    52141 tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa
    52201 aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat aaaatcttga
    52261 tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc ggtaactctc
    52321 tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca ggcgacaaac
    52381 ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt gaaacacaag
    52441 taatcgaagg agacattcct gatggtttga tggctttaga catcggccca gcatcagtaa
    52501 aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga cctatgggtg
    52561 tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca atcgctaact
    52621 tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca gaacaattag
    52681 gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg gaattgcttg
    52741 aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc aagtttgctc
    52801 aattctgacg agtaatcgaa caaaaggtga agggagtttt catgactccc tagccatttt
    52861 tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa
    52921 atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct
    52981 tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca agaattaatg
    53041 acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg ggaaaactca
    53101 ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt tcattatgtg
    53161 atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga catcaataaa
    53221 aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg cgaatcttta
    53281 gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac agctggttta
    53341 gctgatcttt cagctgaaca ggtgtcaaac atggttattg cttatgaacc aatctgggca
    53401 atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt
    53461 caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat ccaatacggc
    53521 ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt tgacggtgct
    53581 ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga cgctgtaaaa
    53641 taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc aaaattaact
    53701 aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga gacaaaacat
    53761 gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta acccaacaat
    53821 cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc cttcaggtgc
    53881 ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc gttatggcgg
    53941 taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag caatcattgg
    54001 ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag acggaactcc
    54061 taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc
    54121 agctgctgac tacctagaag tacctttata ccactaccta ggcggattca acacaaaagt
    54181 attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata actcaatcga
    54241 cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat
    54301 gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat tagcaacttc
    54361 tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag gttttgaagt
    54421 aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg ttcttgctat
    54481 ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac tagctgactc
    54541 aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat tagtttctaa
    54601 atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg gattcaagaa
    54661 attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt tcgtaacaaa
    54721 cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt
    54781 gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta aagaagctgg
    54841 ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa tctctgatat
    54901 cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta ctgaccgtat
    54961 tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg cagaatacaa
    55021 aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg aagatacatg
    55081 atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa ctctctactt
    55141 gctgaaatat cagcaggtag agagtttttt tgcaactagc ttctgtatat gatgttt
//
LOCUS       DAZKSU010000010        53857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000010 DAZKSU010000000
VERSION     DAZKSU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.010"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..1524
                     /locus_tag="ACG8Z3_000625"
     CDS             184..1524
                     /locus_tag="ACG8Z3_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0822971.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1719..2162
                     /locus_tag="ACG8Z3_000626"
     CDS             1719..2162
                     /locus_tag="ACG8Z3_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0822972.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKIAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2424..3842)
                     /gene="nhaC"
                     /locus_tag="ACG8Z3_000627"
     CDS             complement(2424..3842)
                     /gene="nhaC"
                     /locus_tag="ACG8Z3_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0822973.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4246..5469)
                     /locus_tag="ACG8Z3_000628"
     CDS             complement(4246..5469)
                     /locus_tag="ACG8Z3_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0822974.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5501..6340)
                     /locus_tag="ACG8Z3_000629"
     CDS             complement(5501..6340)
                     /locus_tag="ACG8Z3_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0822975.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6357..7037)
                     /locus_tag="ACG8Z3_000630"
     CDS             complement(6357..7037)
                     /locus_tag="ACG8Z3_000630"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0822976.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(7039..8112)
                     /locus_tag="ACG8Z3_000631"
     CDS             complement(7039..8112)
                     /locus_tag="ACG8Z3_000631"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0822977.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(8171..8409)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8452..8685)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8762..9433)
                     /locus_tag="ACG8Z3_000632"
     CDS             complement(8762..9433)
                     /locus_tag="ACG8Z3_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0822978.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9521..12169)
                     /gene="mgtA"
                     /locus_tag="ACG8Z3_000633"
     CDS             complement(9521..12169)
                     /gene="mgtA"
                     /locus_tag="ACG8Z3_000633"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0822979.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASPNGLSEEQIERSRET
                     YGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELITGEKI
                     SEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLMPLPGNYWLWF
                     FITVVAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(12512..12686)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12859..13296
                     /gene="ndk"
                     /locus_tag="ACG8Z3_000634"
     CDS             12859..13296
                     /gene="ndk"
                     /locus_tag="ACG8Z3_000634"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0822980.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(13318..14184)
                     /locus_tag="ACG8Z3_000635"
     CDS             complement(13318..14184)
                     /locus_tag="ACG8Z3_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0822981.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(14418..16496)
                     /locus_tag="ACG8Z3_000636"
     CDS             complement(14418..16496)
                     /locus_tag="ACG8Z3_000636"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0822982.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            16989..18206
                     /locus_tag="ACG8Z3_000637"
     CDS             16989..18206
                     /locus_tag="ACG8Z3_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0822983.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(18665..19288)
                     /locus_tag="ACG8Z3_000638"
     CDS             complement(18665..19288)
                     /locus_tag="ACG8Z3_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822984.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(19444..20841)
                     /locus_tag="ACG8Z3_000639"
     CDS             complement(19444..20841)
                     /locus_tag="ACG8Z3_000639"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0822985.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(20863..21834)
                     /locus_tag="ACG8Z3_000640"
     CDS             complement(20863..21834)
                     /locus_tag="ACG8Z3_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0822986.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(21841..24141)
                     /locus_tag="ACG8Z3_000641"
     CDS             complement(21841..24141)
                     /locus_tag="ACG8Z3_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0822987.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(24143..25468)
                     /locus_tag="ACG8Z3_000642"
     CDS             complement(24143..25468)
                     /locus_tag="ACG8Z3_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0822988.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            25699..26124
                     /locus_tag="ACG8Z3_000643"
     CDS             25699..26124
                     /locus_tag="ACG8Z3_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0822989.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(26622..27323)
                     /locus_tag="ACG8Z3_000644"
     CDS             complement(26622..27323)
                     /locus_tag="ACG8Z3_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0822990.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(27339..27779)
                     /locus_tag="ACG8Z3_000645"
     CDS             complement(27339..27779)
                     /locus_tag="ACG8Z3_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0822991.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTITSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(27779..28354)
                     /locus_tag="ACG8Z3_000646"
     CDS             complement(27779..28354)
                     /locus_tag="ACG8Z3_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0822992.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(28765..29271)
                     /locus_tag="ACG8Z3_000647"
     CDS             complement(28765..29271)
                     /locus_tag="ACG8Z3_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFQ0822993.1"
                     /translation="MDYKKRVQQMFLDSGVVLKETELERIEYTDFGLGAIETEGLNLF
                     VYENNERYCGKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKL
                     SCIIPKGQERYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDS
                     FTNPAIYR"
     gene            complement(29282..29794)
                     /locus_tag="ACG8Z3_000648"
                     /pseudo
     CDS             complement(29282..29794)
                     /locus_tag="ACG8Z3_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(29933..30634)
                     /locus_tag="ACG8Z3_000649"
     CDS             complement(29933..30634)
                     /locus_tag="ACG8Z3_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0822994.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(30742..32184)
                     /locus_tag="ACG8Z3_000650"
     CDS             complement(30742..32184)
                     /locus_tag="ACG8Z3_000650"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0822995.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(32198..34063)
                     /locus_tag="ACG8Z3_000651"
     CDS             complement(32198..34063)
                     /locus_tag="ACG8Z3_000651"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0822996.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(34184..35035)
                     /gene="licT"
                     /locus_tag="ACG8Z3_000652"
     CDS             complement(34184..35035)
                     /gene="licT"
                     /locus_tag="ACG8Z3_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFQ0822997.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(35345..36058)
                     /locus_tag="ACG8Z3_000653"
     CDS             complement(35345..36058)
                     /locus_tag="ACG8Z3_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0822998.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(36126..36452)
                     /locus_tag="ACG8Z3_000654"
     CDS             complement(36126..36452)
                     /locus_tag="ACG8Z3_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0822999.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(36575..36802)
                     /locus_tag="ACG8Z3_000655"
     CDS             complement(36575..36802)
                     /locus_tag="ACG8Z3_000655"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0823000.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(37124..38377)
                     /locus_tag="ACG8Z3_000656"
     CDS             complement(37124..38377)
                     /locus_tag="ACG8Z3_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0823001.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            38438..39178
                     /locus_tag="ACG8Z3_000657"
     CDS             38438..39178
                     /locus_tag="ACG8Z3_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0823002.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(39264..40022)
                     /locus_tag="ACG8Z3_000658"
     CDS             complement(39264..40022)
                     /locus_tag="ACG8Z3_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0823003.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(40019..40483)
                     /locus_tag="ACG8Z3_000659"
                     /pseudo
     CDS             complement(40019..40483)
                     /locus_tag="ACG8Z3_000659"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(40502..40999)
                     /locus_tag="ACG8Z3_000660"
     CDS             complement(40502..40999)
                     /locus_tag="ACG8Z3_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFQ0823004.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(41094..44234)
                     /locus_tag="ACG8Z3_000661"
     CDS             complement(41094..44234)
                     /locus_tag="ACG8Z3_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0823005.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEIT"
     gene            complement(44458..45342)
                     /locus_tag="ACG8Z3_000662"
     CDS             complement(44458..45342)
                     /locus_tag="ACG8Z3_000662"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0823006.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(45437..45874)
                     /locus_tag="ACG8Z3_000663"
     CDS             complement(45437..45874)
                     /locus_tag="ACG8Z3_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823007.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(46091..47002)
                     /locus_tag="ACG8Z3_000664"
     CDS             complement(46091..47002)
                     /locus_tag="ACG8Z3_000664"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0823008.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(47459..47887)
                     /locus_tag="ACG8Z3_000665"
     CDS             complement(47459..47887)
                     /locus_tag="ACG8Z3_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823009.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(47915..48553)
                     /locus_tag="ACG8Z3_000666"
     CDS             complement(47915..48553)
                     /locus_tag="ACG8Z3_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823010.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(48557..50593)
                     /locus_tag="ACG8Z3_000667"
     CDS             complement(48557..50593)
                     /locus_tag="ACG8Z3_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFQ0823011.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            50719..51384
                     /locus_tag="ACG8Z3_000668"
     CDS             50719..51384
                     /locus_tag="ACG8Z3_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823012.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(52875..53747)
                     /locus_tag="ACG8Z3_000669"
     CDS             complement(52875..53747)
                     /locus_tag="ACG8Z3_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0823013.1"
                     /translation="MIYGAIEAGGTKFICAVSTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQRKSKKYGHITSTPKLAWQNFNFVGTLKQHFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMLSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIASYIVTPELGDSAGI
                     IGGLALARKAIRNK"
BASE COUNT        15746 a        11261 c         9034 g        17816 t
ORIGIN      
        1 acatttctat gaaaatacga tgaaataaat cattcttcca ttaaaatatg cccaaaagtt
       61 ttctgaatag ttgtaattat ctgaaaattt gttatcatta gtcattatta ttattactat
      121 ttattcttac tatttattct tactatgcat tttgattgtg aatttaaaag aaagaggtta
      181 tttatggaag ataaaaaatt gaaaaaacaa cttagctccc gacatatcac catgctagct
      241 ttaggtggag cgattggtgc aggtctattt aaaggaagtg gcgaagcgat tggaatcgcc
      301 ggcccttctg tattgatcgc ttttttgatt ggcggcataa ttttatatat cgtgatgaaa
      361 ggtctgggaa aaattgtttt aagtggtggt gatacgcacc acggattatc tggattgatt
      421 cgtccatatc taggtgctca tgctgccgat tttaccgatt gggtctactg gtccatgtgg
      481 attatcaata tcattgctga agctgttgca gctgcttcct ttttacagtt gtggttcccc
      541 cacatcccag cttgggtatt tgtttttatg ttggctgttt taactactat catcaatctt
      601 tattcggtac gattattcgc agagacagaa tattggctgg cctttgctaa aatcagtgtc
      661 attatcctat tgatcatttt tgctgcttat cttgtcggtc agcaaatgtt gggtactggt
      721 gttttcccta cccttcaaca attaactgat catggtggtt ttactcccca cggtatgaag
      781 ggaatcatca gttctttgct tgtagttatc tattcttacg gcggttctga gttgattgct
      841 atcacagtaa gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggcgtc
      901 atgggaagaa tcatttcctt ttacattatt cctttatttt tgcttttgat tatttttcct
      961 tggaatactt tagcaggcac gaacgttagt ccttttgtga tggtatttga aaaaatgaat
     1021 attccatttg cggcagatat cgtcaatttc gtgattgttc ttgctctgtt ctcgtctatc
     1081 aattcaggtg tatatgcttc ttcgagaatt ttgttcttcc gcttgaaaga tcggaaaggt
     1141 tcctctagaa aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgtgctgttt
     1201 tgtaccagtg ttctttatct aggtgtggct ctttcttatt ttgtcggtga caaattattt
     1261 ggctatctgg caggttctct ttcctataca gtattattga tctggatcat tatcagtgct
     1321 gcgtcctttg tactgacact aaggaaagga acgttatggg acaaaggaat cagctttttt
     1381 gcattagcag tcttatggtt gattttcctt ggtatcctat tcacgaactc cattggggta
     1441 actgtattga ccggtttgct ttacctgttc atttatttca gttatcaaaa aaagaatgat
     1501 gctttcgtct tgacgaatga atgaaaccaa tagagaattt tattctattt atattgaata
     1561 tctggagcta ttgaaaaaaa ggagctgcga caaaaattgt tgcagttctt ttttccgaat
     1621 aaacggtgtt caaaaaccat ctgtgcagat taattgattg ttctccacgc tcatgacatg
     1681 atagaagaaa ggaatgaaga aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt
     1741 tgttgatgat tggtgtttcg acggtagtca tgtttggtat gatgtatttg aatgtttatg
     1801 cgcttgatca tatcttcttc agtcgcacac gtgtattcat ggcgttgatg atgggtgcag
     1861 tcatggcagt catcatgttg ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg
     1921 ctatgatttt agccgctagt cttcttgttt ttgccggttc cttatttatg gtacgaagcc
     1981 aaacaactgt caacgatacg gcgtggatga aggcgatgat ccctcatcac tctattgcta
     2041 tcttgacaag taaacgagct gatttaaaag atcctgaagt aaaaaaaata gcggaagaaa
     2101 tcattcaggc acaagaagaa gaaatcgaca aaatgaaaaa aatgatcaaa gaaatggaat
     2161 gaccattatc catccaaata aaaaaagagg atgttccaga agtgatcaac ttcaagaaat
     2221 aagaagaagc tacttctgga acaccgtgaa tcagaggttg tgaaaaagct gccctgcatc
     2281 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttaggcttat
     2341 taccgcaaat gcaagctttt gaacaccgtt tattcgtaaa agggagtgtg acaaaagtct
     2401 tgtcacactc cctttttacg tttttattgt tctaattgcg ctgtcttgct tactgcttct
     2461 tgcggtttca caaatttctt tgagatcccc caacctgtaa aggcactgac catcgaaaac
     2521 acaggtgaaa gcaaactgaa aaacacgaat ggcaagtaag tgagtgtagg aatgccaaat
     2581 gtacttgcta cgaaacttcc ggcgattccc caaggcacta agtagttgat tactgttcca
     2641 ccatcttcta gtgttctccc taatacagtt ggatcaaatc ctgctttttc gtaagtttct
     2701 ttaaatgcat taccaggcag gataacagat agaaattgtt ctccaataaa gatatttact
     2761 ccgatgcttg tcatcaatgt tgtaaaaatc aattttgggg tgttcgtcat ctttcttgat
     2821 actcgttcca taacggttga gatcaaccca aattcgatca acaaaccacc taatgacaat
     2881 gttagaataa tcaatgacac agttggcatc atgctttcga ttcctccacg gctaagtagt
     2941 gaatcgattt gttggtttcc agtatttgaa acaaatcccg attcgatgac ccctgccaac
     3001 ttcgttaagg aagtttgtgg gttttgaata aagatcattc ctactgctac agcaatattc
     3061 aacaaaatcg tcaaaattgc cggtaccttt ttccatgcac acaagagcat taaaaataga
     3121 ggaatcagtg accagatcga aatagtaaag tttgcttcta aaatatcagt cacttcacga
     3181 atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa
     3241 acaacaaatg ccggaacagt tgaccacatc atatgcttga tgtgtttgaa caaatcggca
     3301 tcaacaatag ctgctgctaa atttgttgat tcggataatg gagacatctt gtctccaaaa
     3361 acggcacctg aaacaatggc tccaacaaca agggctggat ttagtcctag tgttgtccca
     3421 ataccgaaaa atgcaatccc aatcgttgac ataacagtaa aggcactccc tacagcgcta
     3481 cctacgattg cacaaacaac aaatacagaa gggacgaacc actggacact aatcaattta
     3541 aatccaatga ccatgagggt tggaataatt cctgcttgta tccatacagc aatcaatgaa
     3601 ccgactaaaa taaaaataaa gattggaatg atccccggtt tgatgccatt gatgatccct
     3661 tcgtgcagcc aatctgtcgg gtattttttt atcactgcgt acaacatgac cattccgata
     3721 gctgccaaaa tcgtgatatt aggtgaaacc tttaatccga caacgccaat cgcaatgcct
     3781 aaaacaatga ccatcaatac aagtgttgct tctaaaaaac ttggtgttcc ttttttttcc
     3841 atgataatct ctcctttttt agttgttctt atgttctaaa aaagttaaga tatcggatta
     3901 ctccgccaca attcatccgc gattcctcct ttataaaaat aaaaagacct gctttcaact
     3961 aaggacgaat gactccgcgg taccacctta attaaaaaca gatctgtttt tcactttcaa
     4021 acgattattc aaatgtatgg actttccatg taattcgtca attaggcttg atcggttcac
     4081 agcgaccacc gactttctga acaaaacacc taaccactac tgcgtgaaaa gttgacttgt
     4141 atgaagttta ccttccataa gatcatttgt caatgaaaaa gaaaatattt tttcattttt
     4201 ctgaaaatta tttttataac tttcgcgcac aactttatct ttttactaga aagtagcgcc
     4261 ctcttgtttt tttcttgcta ctatttcttg atgttcttgt ttgatttttt ctaatagctt
     4321 gggttctctc aataaatcta gagctgtatt ggcaagtaat tccgctgcaa tcgcaataga
     4381 agcaagtcct ttttcacttt tagcagcagc tttgaattct tctgtatgtc cggcaatata
     4441 tgtctcagag attgctacag taggctgtat cgttggaata acttggctaa catttcctac
     4501 atcagaagaa cctagacttg ttctttgttc cgtttctatt tcttcatcag gaacccctgc
     4561 ttcttttgca tgttggaaga ataagtcatc gaaagaaggc gttacgatga catcatcgac
     4621 tgcattttgg aacaatccaa actcaaaagt cgtacccgtg cttaatgcag cacctcgtac
     4681 gatattttcc actttttcat atactttgtc taatgtttgt cgatttcctg cacgtaaata
     4741 gaaacgtcca gcagcgtagt ctggtatgac attggccgcc acgcctccat ctgtaatgat
     4801 tccatggata ttgacatctt ttggcaattg caagcgtaga gcgttgattc cgttaaacac
     4861 ttggatcaag gcttccaaag catttactcc tttttcaggt gctgctgctg catgagaagc
     4921 gacaccgtaa aatttgatat cgactggatc atttgctaaa gattctgtcg ttttcccata
     4981 acgaaaagct ggatggacac ataatgctgc atcgacatcc tcaaaaaaac cttcccgaac
     5041 gaagcttccc ttagctgatc cattttcgcc tccttcttcc cccggagtac catacacacg
     5101 aatttctccg ccaatctcat caatgaattg tttcactatg ctggctgcta atacggagta
     5161 agtaccaaat aaattatgtc cgcatgcatg accaatacct ggtaaagcat caaattctgc
     5221 caaaaaggca attgtcggac ctggtttatt ggaaatataa cgtgcatcaa agcctgttcg
     5281 gtgcccagct acttcttttt ctacccagaa cccttctttt tccaattgtt tgattaaagc
     5341 tgctgaggaa taaaattcat aattacttac ttcaggatgg tcgtggatag ctagtgcaag
     5401 tgcttgatat tctggtaaac ggtattgaat cagatccttc aattgatttg ttgagagtgt
     5461 ggttgtcata aaaatttcct cctatgctag tcgttttttc ttattttctc ccgaatgttt
     5521 cccacgcagg tacgcttgaa ccttttgatg ttttttcgat tactttggct gtttcttctg
     5581 tttgataggc atctactatt ttttgatacg ttttattgtc ttcatcttct tttcttgcaa
     5641 cgatgatatt gacataagga cgtgcatgct catcgactgg ttctaagaag attgcatcag
     5701 aagcgggatt aaatccagca tctacagcta caccgctatt gatgactgaa atatctacat
     5761 ctgacaaagc acgtgctgtt tgggaagcat ctaactcaga aatgtctaga ttttttttat
     5821 tctcggtaat atcagaaacg gtaggcgctt ggccttttgc cttgtccact ttgatcaatc
     5881 cagcagtttg caataacaga agagctctgc cgccattcgt gacatcatta gggattgcaa
     5941 cttttgctcc atctttgata tcagccacat cttttaacgt attggaataa attcccaacg
     6001 gtgcattaac cgtattccca atcgatacta aatcagttcc gtgttcttca ttgtaattgt
     6061 ctaaaaagaa ttgatgttgg aaggaattca aatcgatcga tccatctgct aaagccgcat
     6121 ttggctgatt gtaatctgta aatttcacat attccaaatg gatgttttcc tttttcaaac
     6181 ggtctttcac atcgtcccat acatctgtat cttccccaat gatccctagt tttacggtcg
     6241 tttctttttt ttcagttgta gctgcttttc cttggctgca tcctgccaac caaataattg
     6301 ctacaaatgt aatgactgca aaacgaataa tttttttcat tttttctcct ccattttcaa
     6361 tgtgtgactt ttttaatcag ttgattactg ataaattgac tcaaaaaaac taagaccaga
     6421 atcaataatg tagcgacaat cgttacatct gtttggaatc gattatatcc tctcgatata
     6481 gcaagattcc ctaatcctcc ggcacctact gctccagcca tggtagtcag tccgatcaca
     6541 ttgatgatcg tcaacgctga gacccgaatg ataccaggta acccttctaa caaataaaca
     6601 cgtaaaatga tgccgattgg gctcgtgccc atcgcttctg ctgcttcgat caccccagga
     6661 tcaacttcta gcagtgcatt ctcgatttga cgggcaaaaa acgggacaac gccaaaaatc
     6721 aaagggacta aagctgcttt ggcaccaatc gtcgttccaa ctaagaaacg agtagtcaaa
     6781 gctaaaagag aaagcaagat aatgaaaggg atggaacgaa tgatattgat caacttatca
     6841 agtatttcga acagcagcct gttagctagt aatccatttg gacgtgtcac tactaacgtc
     6901 acacctaaga tgattccgat gatcccacca atgaatgccg tccaaaaaga catatatagt
     6961 gtttgctttg tcgcttcgat gaattcatcc ggaatcaaat agacattcgg caaatatgtt
     7021 ttaaaaaatt cagtcatttt agtttcctcc ttcgatgact ttgatacggt tatctaattc
     7081 atttttttgt aaaatagtta cttttacacc agatttaatc aaataatcca atgctttttg
     7141 ccgctgtttg gtttctccag ctaaagtaac gatcaaactt cctaagggtg tttcttgtaa
     7201 tagctctaca ttaccgtata agatattcgc tgttacttgg tacttgctgt aaagatgagc
     7261 gattagaggt tcgttcgtct ggttgccgat ataacttaat tccaccaacc atttatttgc
     7321 aatctgatcg gcaaaagcag catgggcact gattgtttcc aatgcttggt caaggtgtgt
     7381 cgctgtccga ataaaatctt tagtcaattc ttcttcagga tgactgaaaa tctggatgct
     7441 gctacctttt tcaatgattt tccccgcttc cataaccgct accttgttgc agatttcttt
     7501 gacaacttgc atctcatgag tgatcaggac gattgttaga cctagctgac gatttagttt
     7561 ttttaacagt gcaaggatct gaagggtcgt tttcggatca agtgcactag ttgcttcatc
     7621 gcataacaac actttaggtt catttgccag agctcgtgcg attgccactc gttgtttttg
     7681 cccccccgac agttgtttag gatacgcaga tgctttttct tccagaccga caagttccag
     7741 tagttcattc actttttgac gacgttcctg ttttgatttt ccagaatact ttagagaaaa
     7801 atctacgttg tcaaaaatcg ttcgactttc cattagattg aaatgctgaa agatcatacc
     7861 gatggccttt ctttcttttc gcaattcttt tggagagagc tggcttaaat tcgtctgatt
     7921 gacgatgact tctcctgctg ttggtttctg caacaaattc atcacgcgga ctaacgtact
     7981 ctttcctgca cctgagtaac cgacgatccc ataaacatcc cctttttcaa tcgttaaatc
     8041 tacagaatct actgcctgta tctctttttc tttttgtttg aaacatacag aaatatctcg
     8101 caattcaatc attaacattc ctccttgtct ataaaaaaag catcctcaag aaaatgaaaa
     8161 tagtcatttt cttgaggacg cctttttgcg tgttaccacc tcatgttcaa gctgtgttca
     8221 cacacaactc ctcttcaagt accatcatac tctagcaaga tatcggtcgc agccgtcacc
     8281 gccttacatc tatgctcggg atgaattgct cgatggccat tttccaatag tcgttccttg
     8341 cttcttctca gctaccgaag ctctctgtca aaggttcttc tatcgtactt accatttcaa
     8401 tgcaaataat attcaaaaaa aaaatccttt tactaacata aactatgtca gtaaaaggac
     8461 gacgtatcgt ggtaccacct tttttcggaa gtttccttcc ctcaagccca acatctcaaa
     8521 aagagagatt ggcgttcgtt ttaacggtcg aacatttccg aatttgccta cttatcgaca
     8581 aattattctc caaggccatt ttcagctttt cttacttgac tcttcacacc agccagagcc
     8641 tctctgcaaa gtttccaagc ttactttcct tctcaacgaa atcaatattt tgttgttggg
     8701 aataatctac aggaggaaaa ctgaaaagtc aagcatgaaa tccaaattat tttttctata
     8761 tttatttttc tgttttttta tctaacattt caataacagt tcgaagatct tttccttccg
     8821 caagaaggtt caatttttcc ttctcttcga cgctataatt tcgaacaaaa ctggaatcgg
     8881 gaatcaaaac aacgcctagt cctgcacgtg aagcggctgt cgctcctact aaagaatctt
     8941 ccactgcaaa agctgaaaga gcttctaatc ctgttcgttc caaagctgtc agataaggga
     9001 ctggtgaagg cttgttctcc gatacttcat ctccgaacgt aaacgtatca aagtaatcgg
     9061 ctaattgaaa gtgatccaag atatctgttg ctcttttcgt aacagttgtc gtagcaatcc
     9121 ctagtttcat atgacgttct tttaagtctg tcaatatttc ttttgcatag ggtttcaatt
     9181 ggatagctcc acttgtcagc tgttgataaa aatattcttc acgctttgct cggacttcct
     9241 gcactttttc aggactgcct gctgcctgca ctaaaatatc aaaactttgt ttccagtttt
     9301 ttccgcccca agcgtcaacg atttctttcg ggatatgaat atcaaattgc tggaatcccc
     9361 aaagccaacc atcccgatac gttctttccg tatccactaa caaaccatct aaatcaaata
     9421 ttgcaccttt catctataca ttaatccttt ccaaaacaat acacctagtt tagcatgact
     9481 gatccaaaat gaacagaagc atagagccca gccgcatcac ttataagagt tcttggaatc
     9541 gacggatata gattttcttc acagctgtca caagcgccag ataagcgacg actgttataa
     9601 aaaaccataa ccagtaattg ccagggagcg gcatcaagcc taactcagca ccaaatcctg
     9661 tgaatggaag gactgaaccg acaatgattc ctaatgtagt gataaccagc atggcaaatg
     9721 ttgcattgct ttggataaat ggtattttcg gtgtacgcaa tgcatggagt actaatgttt
     9781 gtgtccacag ggattcgaca aaccatcctg catggaataa tgcgataaaa atcacttgtt
     9841 gtgagctatc caatgtatgg aaacttccac caactacagc aggacaaata acaaaataca
     9901 tcaacaagta agtagtgata tcaaaaatcg aacttgttgg accaaaataa accatgaatt
     9961 ttccaatact agatgcattc catttctttg gctcatccaa atattcttta tccatccgat
    10021 cccatggtaa ggaaatgcaa gaaacgtcat agatcagatt caaaaataga agttgcaaag
    10081 gcaacattgg taaaaatggt aagaacgtac tggcaatcag tacggaaaac atattgccaa
    10141 aattagaact tgctgttgcc ttgatatatt tcatgatgtt tccaaaagtt tctcttccag
    10201 aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgctgatt
    10261 cttttgcgat atccacagct gtatccacgg aaataccgat atcagccact ttcattgctg
    10321 gagcatcatt gataccatct cctaaaaatc cgacagtatg cccattttta cgtaaagctg
    10381 tcgtcaatcg tgctttttgc tgaggattca attttacgaa tacttcgtgt tcttcagcta
    10441 cttgcgctaa ttcatgatca ttcatttccg agattttctc accagtaatc aattcattga
    10501 tttctaggcc tacttgacgg cacacagacc gtgtgactaa ttcattgtcc ccagtcagaa
    10561 cttttacagc tacaccatgt tcttttaaag ctttcaacgc ttgttttgtt gtttcttttg
    10621 gtggatcaag gaaagcaaga taaccaatca gaaccatgtc tgactcatct ttgactgaaa
    10681 attctccaac aacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccgtctt
    10741 cattaagtga ttgtactttt gcaagcaccg cttcttttcg ttcttttgtt aatggtgcga
    10801 cagtaccacc aagatcaata aatttagaaa tgttcaacat ttcttcaatc gcgccttttg
    10861 tgatcatctg tgttttgcca gccggatcag cgactacaac actcatacgg cgacgttcaa
    10921 aatcaaaagg aatttcatct actttttgat aattgattgt atctgtatct aaagtttctg
    10981 ctgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctgt
    11041 ttagataagc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt
    11101 caagaatgat tttgtcttgt gtcaatgttc ccgttttatc cgtacataac acatcgatcg
    11161 caccaaaatt ctggatagca tttaaatttt tgatgactgt tccttttttg gccatcacac
    11221 ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggc gttaaaccaa
    11281 ctgccacaga taatccaaaa agaaaagctt ctaaccaatc tccttttgta agcccattga
    11341 taacgataac tgctggagcc atcaatgaca taaatttgat cagcagcatg gatgttttgt
    11401 ggatgcctaa ttcaaaactt gattggatgg gtttttcaga tagcgtttct gctacatgcc
    11461 caaatagtgt gtcatttccg acagaaacaa tgattccttc cgcgcttcca ctgatgacat
    11521 tgcttcccat aaagacaaga ttttcataac ttgtttcact ttgctgatcg tcttcacata
    11581 ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttga gaaatgaata
    11641 gatcctttgc ggacaataaa cgcatatccg ctggtatcat atctcctgct gaaagccgaa
    11701 caagatctcc acagacgatt tcttccatag gaatctctgt atactcgcca tttcgtttga
    11761 cagctgccgt tactttcaca agtgatttca gtttttcagc tgcttgatta gaacgcacag
    11821 actggatcaa cgtcatcgtc ccactgacga taaccatagt gaagataatg gctacaccaa
    11881 agagatcttt atctcctgga gcggctaata cgtattccgt aatgaatgaa ataattccta
    11941 atgcaatcaa cactaatgta aagggtgtga tgtatgcttt caatatttct ataatgaatg
    12001 gtgttttttt gccatatgaa atcgcatttt caccataagt ttctcttgaa cgctcgattt
    12061 gttcttctga caatccattt ggtgatgcat ggaacaattc aaataattct tgcttcgttt
    12121 ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc tcgatctctc
    12181 ctttgtgtcc aaaagacagc tgttctcgct gtttttggtc ttttgctatt catcggatcg
    12241 atagagaaaa tgctgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta
    12301 aatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt
    12361 taattcatga cttccaatca tttttcgaat agttattttc ccaactcgct cacctccctt
    12421 tttgtttttt cacctatggg atttgcggca acaaaaaaac ctctcgccaa atactcagcg
    12481 aaaggtttga aaaagcaaat caaaaaaagc ttctcagatc tcatcgttga gttttgacac
    12541 tgtacaacgt aaaaagcaga tatctgcttt tagctacctt atgaaaagcc ttatctcgac
    12601 aattcctgtt ctacccattg gcgtctctcg acgtttttgg gcagtagcct atgtttgtat
    12661 aggagcctca cctaacaaat cctattcggt tttccctcgt taatataaca actaaaaaag
    12721 aggatgtcaa atcaattttc catgtttttc atactttttt aaaatacacg cgttatattt
    12781 cctgtctcac gcagttttat ttgtctcatt ttccacctcc cgctataata gaattatagt
    12841 aggaaacgag gtgctgttat ggaacgaaca cttgttatta tcaagccaga cggcgtaaga
    12901 cgccatttgg tcggaagcat catccaacgt tttgaagcaa agggactggc cattgctgaa
    12961 atgaaatttg caacgatgac acctgaacta gcaaaagaac attatcagca cttgactgaa
    13021 cgtagttttt ttgatgagct tatcgattac atgacttctg gtccggtcgt ttatcttgtg
    13081 ctggtcggcg aggaagtgat cgatatcgtc cgtaagatgg tcggagcaac aaaaggggcg
    13141 gatgccgttc caggtacgat ccgaggagat tatgcactgc cggggactga aaacatcatc
    13201 catgcttctg attcccgtga tgctgcaaca aaagagatcg cccgcttttt cccgccattc
    13261 gatacaaaca aaccgtctga aaaagcggcg aagtgataca aatcctaaac tgttggtcta
    13321 actgctgggt ttagcaatgg ccagcagttt tttctctaaa gaattccttt gattcttccc
    13381 taatgtatat tcgatcactg cgaaaaactc ttggtgattt tccgattttt cttcccctaa
    13441 agcagcagaa atcagtcgtt ggtaataatc cgccatcaat gtttcataaa tcactgtact
    13501 tctttcttta tgcgctaaac tccgtttcat atcttcattc acgctacgtg ccttttttaa
    13561 gcgaccattt tctatataaa acaaagtcaa gttcgtcaaa aaagtaaaga gatactgctc
    13621 gtgattattc agttgcgcga tccgtcgcat tctttttttg attttcaaat aattattctc
    13681 aataaaacga tcactgaata tccaaggaaa atctgctaga atagatactt caaattgccc
    13741 atactctggt acttgcatca aatattcttc aatgatacga cattcataac gcaaaaattc
    13801 aaattccgtc tcagatactt ctgttttttt ggacatgtgc agattcaaaa ccaaatagta
    13861 cagatcttct gttttcttat agtgcctgcg gttattagca atttctttgt aaggatcaaa
    13921 gctgtcttct tgaatccgat ctagcattcg tctttttttc tttaattttt ttggctcatc
    13981 aagctgattt cgtatccatt catattcggc aagtgtcatg ttcattcgct ctaaaaatga
    14041 tttcaatgtt ccaaacgata cttttttcac tgaatttgtc tcgatcccga ctagcgaagt
    14101 tttagaacag attccttccg ctaattgggt ctgtgtcagc tttttgttga ctcgaagctt
    14161 cttaatcaat tctccatcat acataatagt tctccttttt taattgaaat taacccttgg
    14221 cttttccaga atgcttgcca ataagaggaa tgattatttc aatttaaatt ttccgcattc
    14281 aataaataga tacgttaaaa ataaaaaata cacgaaaaaa ataaaaaaat gtttttctca
    14341 taaaaaatac caaccttcaa gcatgagact gctgtctaat ccctgaaggt tggtttgatt
    14401 gtttattgat cattttttta ctgctccgca ttggttcctg ttactggata taggatagtc
    14461 gttgttttat tgccgttttt tgaaaagaca ctggtcgaat gttcaccttt ttgcttttcg
    14521 attgcttcat tacgttccgc ttcgttttta tagtcatatt ttttcggatc aatcgtctca
    14581 aatccgtttg gcacataaaa ccgcaagaga ttttcctgat tcagcttatc tgaaagactt
    14641 aacgcagtct tgactttttc ctgatctttg gagatttcct cgtcgattcc tgctgctttc
    14701 gtatcgatga tttctcctgt ctgattattg taagcttttc ctcctaggac ggtgtatttg
    14761 ggcgtcacga agttcccatt tcgaaaagca actacttgat cgtgctgaga agataataag
    14821 tctgtaccaa aatgaagata atctttatca tcgattccca atagatgtag taatgtcggc
    14881 aaaacatcga tttctccacc atattcatga ttgactgtcc ctttagtgta accaggcata
    14941 tggatcatca atggcactcg ttgcatctgt gcgttgtcaa attcatccca tgtatctgga
    15001 tctttaccaa gagcacttgc taaatcttta ttatctgtat tcgagatccc aaaatgatcc
    15061 ccgtaaatca caaagattga attttggtaa ataccagatt ttttcaaata cgtaaaaaat
    15121 tgttccaatg attgatccag ataatgggcc gttcgaacat aattatctac tgtgctattg
    15181 ccagtattca aagatggaaa gtcgaaattc ttgtcatccg tataataagg cgtgtggttg
    15241 gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat gttcaagata ttgggcagat
    15301 tctctgaata aatctttatc taatatccca taacctaacg tttcatcgga ttcatcaaaa
    15361 taagatcgat caaagaaatt gtcataccct aaatttttat atacatgatc tctattccag
    15421 aaactagcta cattcccatg gaaaacagca cttgtatagc cttctgtttg accaagggtc
    15481 gctggtgctg cttgaaagac attatctgac ccaagttctg tgaataatga gccttgaggt
    15541 aaaccaaatg tgccagtttc caacatgttt tcagcatcac ttgtctttcc ctgtcctact
    15601 tcatgaaaga aattgtcaaa actgatcgtt gcttgattct ggaaaatact atttaaaaac
    15661 ggcgtgactt cttgtccgtc taccttcata ttgatcaaaa actgttgaaa actttctaaa
    15721 tgaaggacaa tcacattttt tcctttagca atcccaaatg tttcaggatt tggctctgca
    15781 taatgtttct ttgtatagtc taaaacttca tcaattccat tactgcttgc atgcgcgcga
    15841 acactgctag tcattcctgt tttgattccg tcataaatag tgtacgcatc gattcctaga
    15901 tatttgacaa tataggaacg gtcaaatgtc cgctgtagca attggggacg gttggcttcg
    15961 ctcaacgcta aatttcctga aaaaatcaat cctgaaagac aggtaacagc gatagccatc
    16021 ggtttgctga cagggttgct tttgataggt attttcttga ccttcaacca aaccagtagc
    16081 ccaatgaaca cagcgatatc caaccaatag atgacatcat ggggtttcat caaagaaaag
    16141 gagcttcctg acaacccttg tgacactttc gaaaagccta atacagattt gactgtaatg
    16201 aaatctgtga attcccgata aaaaataata ttcaagtaca acaaaagcgt atttaaaata
    16261 tcaatgatca aaagaatggc taacgctggc tttggctttt taatatacaa agaaaggcca
    16321 aaaaacaata aggtagtcgc aatcgggttg atccacaaga taaagtactg gatcgtacct
    16381 tccacaccta atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa
    16441 acagctgtca aaacgattgc taaacgattt tggcacaatt ttttaagttg tgtcacgtat
    16501 atcacttctc tctattcaca ttttttctta ttttgctcct gaaaaatgaa aatttcctaa
    16561 aaaagttgtt atcttaagat ttttttattc tttctaaaga ttattccatt catttttcac
    16621 agcataaaat cgtttcggtg taagcggtta aaatagtaag gagataaaaa tcttgtggtg
    16681 accgatttac tctatagtat tcaaaagtag tttctcggta ttcgtacaaa aaaaacatac
    16741 taagattaga ggggaacatc attgacgaat gatgttctcc tctattttct atttttagat
    16801 tatagtgaag agtgaaagaa agttcgaaca aagccttgag gactacgagt ccctatcaaa
    16861 aaccgaactt ctttactgtc attttagaat caggtttacg tatgttgggg ccatgagccc
    16921 gtgtatagga aattggaatc gaaatgaagc agtaaagttc tttcaacact taaaaaggag
    16981 gaaaacaaat gccttacatt cttgcattag atgtttcaat gggacacagc tacagtgtcc
    17041 tatacaaaga cgacacctgc ttatttgaag acgagatcga acacactcaa agaggatttc
    17101 atgcattact cgaagagatc cagcacttgc ctgaacgtcc attgattgtt tttgaagcaa
    17161 cgggtattta ttccagacca gtcgaaaagt tctttcaaga tcatcacttt tcttactgtt
    17221 tgttaaaccc gttagaagcg aaaaaacaga tggaagaaaa caccttacgc agttggaaaa
    17281 cagataaatc ggatgcccat cggctcgcac agactcatct taaaaatcaa cgtcaaccaa
    17341 aagaagtaca aagcaatttt tacttagaga tgcgcgattt agcccgtttt tatcaagaaa
    17401 ttgagaagaa aatcactcga ctgcgcatgg atttacacaa ctgccttcag ttgacttttc
    17461 cagagcttga acagttcttt tcaaacaggc tgacccctta cgcattgaca ttgatccgtc
    17521 tttttcccca tccagatttt gtattggcat ctactcggac aaaaataaaa aataaactga
    17581 tcaacgaaac acgggaaaaa atctccgcga atcgtgccga gcaaaaggca gatcaaatca
    17641 tccactatgc acagtgtgcc tatccagctg ttgaaaaaga cagtattcac tgtcaaaaaa
    17701 cgatctatta cgccgaactt ctccaagatt tactcgaaca aaaaaaagca ctggcgacac
    17761 aaatgatcaa aaaagccgaa gcctctcctt gctttttgtt gtaccaaacg ttccctggga
    17821 ttggcgcact cacagccgct ttattgctcg gtgaattagg agatatcaca cgatttaaaa
    17881 cccataaaca attgaatgcc tttgtcggaa tcgacatccg acggtatcat tcaggtaagt
    17941 atactggcca agaccgcatc aataagcgtg gtaatcccaa agcacggaaa attattttct
    18001 tcaccattcg aaatatgatt cgccagcaac gcgcagctcc taatcatatt gtcgattatt
    18061 actacaaatt aaaaaagcaa cctatcccta agaaagataa ggttgccaca gttgcttgta
    18121 tgaacaagct tctgaaatgt atgcacgcta tggttagggc acatacagag tacgactatg
    18181 cgtacgcggt ctcagtggac cattaactaa accacttaca gtataccact tctttgccca
    18241 gatcgaaaat tttttacgaa cggggcttta tttagtgtgc cttgattttg tcgctttcgt
    18301 ttacgagcta tttttcttgc accttttact tgactaatcg taggaaagag gctgtgacaa
    18361 aattcttgtc gcagctccct atttcttgat gcaggacagc tttttcacaa cctctccact
    18421 aaacggtgtt caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggag
    18481 ctattttccc gtttttttct ctaatactca ggtcaaaccg ctgatttcac aacctctgat
    18541 tcacggtgtt ccagaagtag cttcttcgga aataagccga aattttacaa aaacttgaga
    18601 agcacttttc gtaaatttct tcttatttct tgaagctgac cacttctgtc acaacttctg
    18661 tttttcattg tccataggtt tgatattgtc cttctgatga ctcattcgta atgattgagg
    18721 cttgattttc ttcatttccc gttatcgttt gtccactgcc agaatctgaa gggcttagtt
    18781 ctgtcgactc gtttttttga gaagaagcag ttgttttctt gacttgttcg ttggtctttt
    18841 tccattccgc tagtcgatcg gccaccactt ttttgtccac tttttcagaa taagctgtaa
    18901 ccacttttcc atttttcaaa cgtgctacct tgtcgccatc atgagtcaaa agcaaaacga
    18961 ccaaatgttt gtctttgaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
    19021 tattgataaa ttgattttct tcttttcctt ttggcaaata gatgtccaat tcaccttttt
    19081 cgtaattggc agttaattta tattcctccc accaataagt tgagaattct tttttgaaat
    19141 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
    19201 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
    19261 agagaagaaa cggtcttttt ttcctcatgc cttcctccac tcctatttaa ttttgactat
    19321 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
    19381 tgaacttctt tttcctgaag cggtaagaac gggaattttc atacattctc tcttggctat
    19441 tctttaagct tcaaatccat ttctgtctga tacatctttg aaccaatgac ccgatttctt
    19501 gatcgttctt tttccatctt gtgctaaatc tactgaaata aaaccgtaac gattcttata
    19561 ggcattggtc cacgaccagt tatccatgca tgtccacata tgataaccta aacaattggc
    19621 accttcttga atggcttgat ggacatattt cagatgagat tgaacaaatt cgatccgata
    19681 atcatcgtgg atcataccat cttccttgat aaatcgtgct tcattttcta cacccatgcc
    19741 gttttcagaa ataaaacagt cgatattccc ataatgatct cgtacattga tcaaaatatc
    19801 atagattcct ttttcataga tctcccatcc gcgataagga ttcattttac gaccaggcat
    19861 ttcataattg tcgaagtaat cctcaggcat aggaccgttt ttagtatcga ttggtgtttc
    19921 ttttgctttt attcggcgtg gttggtaata attaattccc aaaagatcta ctgtgttttc
    19981 tttaatgatc gtcaaatcat ctggatgaat ctccggcatt agatcgagtt cttttattct
    20041 ctcaatcaat tcactgggaa actcgccttt tacagctgga tcaaggaatg aacgattgaa
    20101 gaacgcatct gctattcgtg ctgctttaac atcttcggga tttgattcgt ctcttggata
    20161 actcggtgtc aaatttaaga tgattccgat cacaccgctt tgatccattt catggtaagc
    20221 tttgatggct aatgctgaag ccaacgcttc atgataacca acttggattg cctttttcat
    20281 atctgattct tccggataat gccatcgata caaatagcct gcctcgactg gaacgattgg
    20341 ttcattatgg gtaaaccatt tttttacttt cgagccaaac aattgaaaac aactttttgc
    20401 gtatttttca taggcttcaa ccgtttctcg gttcaaccat ccgccttttt cttgcagata
    20461 catcggcata tcgaaatgat acagattaac aaagggttcg ataccgtttt caatcaactc
    20521 atcaaaatag tgatgataga aacgaacggc ttcttcgttg attgttccag attcatcagg
    20581 tatcaaacgg ctccattgga tagaggttcg aaatgaatta tgcccagtct gtttcatcaa
    20641 ctgtacatcc tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc
    20701 aaagaacttt tgcggttcgt ttttgtacca ataatcaaaa atattctctc cttttccatc
    20761 gcctgaaatt cttccttctg tttgtggacc acttgctgca gagccccacc aaaaattttt
    20821 tggaaattga tatcttttca tcaaagcacc tctttttctt ttttagttga ttccatgtat
    20881 gatattttcc atcacaatca ctttttcatt ttcttcctct tcttttttga ttcgtttcaa
    20941 cagctgttct gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg tgatttttgg
    21001 aaggatactc agatcgtttt tatctaaata atcatagtga ctgaacgcaa tatccttccc
    21061 gattctgaca ccttcttgat tgaaacattc gatggcacca atcgacatgt tgtaattgct
    21121 gacaaaaata gcagttgccc ctttttctaa taatgctttt gccccgtaat agccgctttt
    21181 tctagaatag tctcctgtaa agatctctaa atggatagac ggatcctttt gtaccgcttc
    21241 tttggctcca gctaaacgtt ctcttgctac gtaatctgtt tgagcggcag caataaatcc
    21301 tattgtttta tgtccttgtc cgccatgatg agcaatcact tttgctacac tttctcgatc
    21361 attcactact attgtatcaa tacctgctgg cccacccgga caattcaatg cgacaactgg
    21421 gatactcatt tttgtcagca tttcaaatcc agaccactct tcttctgata agaagacgat
    21481 caatccatcg atcgcatgct tcatcaggta ttcgattttt tgttcgattt tttctggctg
    21541 atcggcaaac ccgcttaata ataagctata cccattttct tcagctactc tttcgatccc
    21601 tgaaacaacc tccgaaccaa atcggttaga tatcccgttt atcaacagtc caatagagaa
    21661 acttcgatta ttcttcaatc ctttagcaat gatgttttct ttatagtcca acgctgaaat
    21721 cgcttctgat attttttcca tattcttttc cttcagctgc tgaccgttta aatagcggga
    21781 aactgttccc actgaaacac cagccatctt agccacgtca cggatcgatg ccatacattt
    21841 tcactcctgc cagttacatt tgatttcttt ttcttgttgt tcgaccatga tgaccgcttt
    21901 ctttgcataa tgccgcttgt catcaatatc catataacta aagtcttcag aagtgagttc
    21961 aaaaacaagc agtctttcct cttgagaccg caattcatct ttacgaaaag ctttcaataa
    22021 tttcgttctt tggatgacaa cgccgtcaat taatcggaca tagacaagag tacttgtttt
    22081 acctgaaaca cttcctgtat ttttaacttt aattgatatt attactttgt caatatccgt
    22141 tttgacttcc atctgactgt attcaaatgt tgtatagctt agaccatgtc caaaaggata
    22201 aaatggactt ccgcttaaat cataataatt ctcttgttta gaagcagcac gctgatagta
    22261 ataaacaggt agttgccgac tatttctagg ataagacaaa ctcaatcgtc cgcttgggtt
    22321 atttcttcca gaaatcatct gcgcaatggc ttttccaccc tcctgtccag gaaaccatgc
    22381 tgttgcaatg gcattcgtaa atgactcgat tttcatcaaa tcgtagggtc ttccttgcac
    22441 aagtatcgtg atgattggct tgcctagttg atacaattgt tcaatcagct gttcttggta
    22501 tccacctaaa gaaagagaag caacatctac attttctcca gaatccatgt tgatcccttt
    22561 cgatgaaact gccccgttac gaagaaattc catatcaaag ttccgcgcac ttgatccacc
    22621 tagaactgca atgatcacat ctgagttttg agcaagggtc accgcttcta ataatttttc
    22681 ttcctgatcc tcctgatttc ttacttcaca tccttgtgca taagaaacct ttgattgggg
    22741 aaactctgtt tttatctctt gtactatctt ttttttcaaa cggtcttctg tttgtggtgc
    22801 agagtagtct cccaacaagt ggtaaacatc gtgagcattt ggacctataa cagcaatgtt
    22861 ttttacttgt tgatccaacg gcaatatacc gtcatttttt agtaaagtga tactttcttt
    22921 tgctgtctct aaatttaagt gctctgattc ctccatgaga tgatcccaat actcctttgg
    22981 atcgtttgca aatggctgtt caaataaacc taataaaaat ttgacagata aaactcggcg
    23041 aactgcttga tctagtagct tttcatctac gacttgatta tcaatggctt ctgcaatctt
    23101 ggtataggtc tcatcccaca aactgagatc gattcctgcc gctaatgcat atgctgcagc
    23161 agtcttttta tctgtaaaaa catcggataa acgatccaaa gccacaccgt ctgccataac
    23221 gattccttga taaccgatct cttttcttaa tgtttgctca aaaagcgctc gattgatatg
    23281 acaaggtatc ccgtcgatgt cattataagc agccatgaca cctactgcat ttcttgtact
    23341 cttcaataat ggtccgtaaa tatcggagaa ctctctgcgt ccaatcgtta ctgtgcctga
    23401 attatgaccg cctaaagctt ctccttgtgc gatacagtgc tttaacacaa caccgatctg
    23461 atccttcttc ttattgattt cctctactgt ctgatcaaga aaagaaactt tttctgaaat
    23521 aatctctcct tggaaccctc gaacgaccgc ttcagaaaaa cgagctgaga gaatcggatc
    23581 ttccccaaag cactcttctg tccttcccca acgaggatct tttgccagat ccaaagtcga
    23641 caccagcgct aaatgaacac ctttagcagc cagttctttt gccatcaatc gactcgattg
    23701 ttctaataat gctacattga aagtatttcc acggccgata ttcgttggat aagaaatccc
    23761 atccaagcct tgatgaccat gtggacattc ttctacaagc aaaactggaa ttttccatcg
    23821 cgaatgctgt agtacatatt cttggactag gttagccacc ttccagcttt cttttgcagg
    23881 aatcccattc ttataatctg ttttagacca cggatcagag cgaaacagac cgtataaggc
    23941 acccagaccg cctccccatt taacatgttc ttttaacttt tctgttaatt cgatcttgtc
    24001 tccgtttttt tcataacatt cccaaccata caaatgctgg tttacctgtc ctactttttc
    24061 agcaatattc atcctcgaaa gtaaatcttc tactctttgg cggatggtta actttgggtt
    24121 ttgatacggg taatcgtaca ccttaatatc ctccttctgc caattttgcc ttattagcgg
    24181 ctaatacaaa tggcaggaat aaaaatacat ctagaatgat aagtattact gttaagactg
    24241 ctgctcgtat atcgcctcct gtagcaataa aagattgtat aaccggtgga gtgatccaag
    24301 gtgcagcagc aactgtttta ctgatgattc caagacttgt ggctgcgtag gcaataacca
    24361 agttaataga tggtataata gcgcatggaa tcatatatat tggattcatg actactggta
    24421 atccaaacat aattggttcc gtaatattaa ataaacaagt agtcagacca aatttagcga
    24481 ttgatcgctg ttcttgacgt ttagaagccc acaagatagc aataactaac ggtaaaatac
    24541 acccgccacc tccgatcaat ccaaatgcat tggtaaaggg ctccgtaata atattaggaa
    24601 tttcttttcc tactgaaaaa gcatccatgt tttcctgaat agactgtaac aaaggtgctt
    24661 gccgtatagg agccaaaata ctcgaaccat gcataccaaa cacccataag aaatcagaga
    24721 aaaactgtaa aaataagatg cctgggacgg ataaaacaaa agctttcaat ggtgtctgaa
    24781 tcgctaattc aattaattgc ggaacattca aaccagtcgt atggtttaaa gtaaaaacga
    24841 caactgaaaa aaataaaatg acaactattt ctggtactaa agaattaaat gacgcagcta
    24901 ctgccggtgg aactccttct ggcatcctga ttttaaaact cttcattttc gctaatttga
    24961 taaataattc tgtactgaca agggcagcaa tcatcgccgt tgctaagcca gacgaagaag
    25021 tcagactttg taagaaaacc cctgtagctt ctgctgtatt tccgtccatc aaagtgactg
    25081 tacttgataa agacatcaaa ataaacatta cggctgtact tagaattcct gcatgaacag
    25141 gactaaatga tggatcatca atcacgcctg tttggatata gtgggttgcc aaattccagc
    25201 cgatattgta acacacgata atagctaaaa tactcatcgt accattattc acgattgaag
    25261 caagttcagc caatggactt agatcaagta ttctttggac tgttgcattt gaaaaaatca
    25321 gagcattcag caaaaccatg acagaagcag ccattactaa cggcatagta acgataaaac
    25381 tgtctcgaat ggccagcaag tgtcgctggc tactcatctt agatacgatt ggtagcattt
    25441 tttcagatag tctgtctacg actttcatta tttcatctcc tagattctaa aattgaaccg
    25501 gttcattttt aatatagcac tttttttaaa gattgcaaca cattttttta aaacgctttc
    25561 taaaggtggt ggatttttta tgacttttga aaaattattc tatttaatgg tattttgtga
    25621 attttttctc ttccatccgt ttgaagtttt actttccaat ggtataattt ctttatccta
    25681 tcaaatgggg gaagtatttt gaagaaatta atcgtactat ttattttgtc tgcgggactt
    25741 ttgacattga ctggctgtga tttgtttggc ggctctgacc aatcctcgga cgaaacaacc
    25801 gattcttctg tcttaaaagc ggaaaaagca gatatcacca acagtgtata tacgctaaaa
    25861 gtacgtaaag atatggattt atccgcggga cagttgacat ttacagataa aaaaatggaa
    25921 tggacacgca cgtatcagac acaaggagct gattcttctg aatcatctga agaaaaaaca
    25981 gtgctgacag atatcaagat tactaccaaa gatgacgaat atattatttc aggaaaagaa
    26041 aataaaaaag aagcaacagt cacatttacc aaaatcggta attaccgttt gaaggataag
    26101 gatggcaaca tctacagtct atagtgccat ctgttggatc ttcttttgtt tctccccttc
    26161 ttttctgcta tagtaaagaa gcaagatgac tgtaaaggag acgaaatgga tgatcgatag
    26221 tcctgattat tgtacatgta ataacaacaa aaatatccat acagaattta atgatcaagg
    26281 aagtttgagt atatgcgaca attgccacaa actagtcaaa gattctttta aatcgtttga
    26341 atcttatcct gtcaaaaatt atgtaaaaag caaaagaaga taagcaaaat caagcaaaac
    26401 tcactatggc tgatttttat gaacttcaaa ctcagcttct tcctttgatc aagcttcaaa
    26461 aactggaatc agtaaaacaa acagtggatc atttcagttc acaaatcaaa gagtaacgtt
    26521 ttatttattt cacatctttg aatataaaat aaccaaacga tagaaagact tattctacat
    26581 cacgtagtaa tttttctttc ccgtcgtttg gttttttact tttagcctaa ttcttgacct
    26641 ttcgcatgct gattatctgt aaaatatttt ccagcaggct ttccttccaa ttcagctaat
    26701 ttttcttgga tctgacgaat cacctttttt gcatcttctg gattttctac aaaatcgaat
    26761 gtatccccat ccacttgtat tttaggacat acatcaaaat cctcatacca ttgattatag
    26821 cgggtgttca gtgtttcgta ataggtgtat aacgtttcat cgctttctag ctgttcatag
    26881 tcacgcccgc gttttttgat acgttctagc atcgtgtcca aagaaacttt gatatggacc
    26941 atcaaatcag gacgtttttt tggagcaaca tcatctaact cgtttagcat cgtatctaat
    27001 aagctatcgt actgctgtac ttcgatatcc gttacccgac ctaaatcagc atttagatgg
    27061 aacaacagac tatcttcgta gatggagcgg tctagcacat tgtcatcgtt cactaaagca
    27121 tttttcattg ctaaaaatcg cttattcaaa aagaagattt gcaataaaaa tgcgtactgc
    27181 tcaggatttg aataaaaaag cggcaatact tcgttatctt cgacattttc ataaaatggt
    27241 ttagaatcaa tctcttttgc aagcatatcc gtcaaactgc tcttacctgc accaatcgtt
    27301 cctgaaatca aaatcactga catacaaccc ttcctcttct ataagtactc gcctacgtaa
    27361 gtttcaacag gcaatttttc aaaatcatat tcacgtacag cttggtctga ttttaaatac
    27421 gtgtgaagac tttgtgtaat catcaagttc gtaggtaccg tctcttcttg tacgatgatc
    27481 atcggttttt tcaacatagt tgccacacct aattcgtaag ccgttcctgg atcgattgtt
    27541 tgatgctcaa agtcaaggat agccaaaaca aattctgcat ttgtcaactc ttccatatct
    27601 ttttcgtata cttcctgcgc ccatccagcg ctgaatagtt cataattgct ttcttggtga
    27661 tgacgtggag agtagaaact tgtgattgtt ttgttttcct ccaacgcttt ttcgatacga
    27721 cttactcgat cgatttgctc ctctgaaaaa aatggtcctg ctaaatatat ttgactcatt
    27781 attttgcctc cttcaataat aactgatttt taatagatac tgctcgtaat tcattgacca
    27841 gttccaatgg attgttggaa aagatcactt gatccaacca ttttttatcc ataaaatcct
    27901 cttttgcaaa agtttctaat aattgttgca agccgctgta ataaccgcca atattcaata
    27961 ttccgattgg cttatcaggt gtcaggccga tcgctatcca agaaagaagt tgcgaaaatt
    28021 cctccaatgt acctagacct ccagggaaaa cgaggacagc atctcctttt tcaaataata
    28081 atacctttct ttcatcaatt gtctctgtag gaataaatgt agtaacatct ttcccaggca
    28141 gctcagtttc aaaaaggcca tttggataga tacctgtgac ttttccgtta ttttccagta
    28201 ctgttttact aatcactccc ataatcccgg agtatgaacc accgtaaacc agttcgattt
    28261 cttcttgtgc catatatttt cctaacgttt gcgcaataaa tttgtaactt tctttattgc
    28321 caaaacggga accgcaaaaa acagtaagtt tcataaaaaa acactcccta actcaaaagt
    28381 ctatcttcat tctacacgtg tttttttaaa ataaaagact agattttgta gttttttagt
    28441 ggtatataga tcaaacacca ctatatatag tatttgcaaa aaaataattt ttcagctatt
    28501 ttacagtaac caaaaaaata aataactagt aaatataaaa ttgaaacgac ttacttatag
    28561 aagaaattgg gatttcgtcc tgcatgtcaa taacacgcat tatttaaata gtcacacact
    28621 tgtttctcct attaatgaaa aatccatttt attctcgtgt tatgtttcac caacgcctga
    28681 tgtttctcta aataaaaaag ccgacgaacc aaataatcga aatttgatca ttttggttca
    28741 tcagcttgtt cccaaacctt tgtattaacg ataaatagct ggattcgtaa aactatcact
    28801 tggatcatca ctgctggaag aaaattcggt gataaccgcg cctgttgcac ctgcctggaa
    28861 ccagtgttta atattttgtt cgatcgtgta ctgatctcca gcgtgaagta tgatttcttt
    28921 ttttgccgta tagtaacgtt cttgcccctt tggaataata caggaaagtt tttttgtttc
    28981 ttttccttcg acataaagat atactaagcc ttttcgacaa cgaaaggttt ccttttttcc
    29041 ttcattctca tctcgaggtg ggtgccgatg ctctggacaa gtttgataag gcaataatac
    29101 cagttctttc ccacagtatc gctcattatt ttcataaaca aataaattca atccttcagt
    29161 ctcgattgct cctaatccaa aatctgtata ttcgattctt tctaattctg tttctttcaa
    29221 tactactcct gaatctagga acatttgttg tactcttttc ttgtaatcca ttctgttcct
    29281 cctaactgat tgtcaagtca tcccaagata tctcttcttc ctctttttct tcttggatga
    29341 cttctgcatt tgggtcaact ggttttttca ataataaaat agttattccc gtagcaaaag
    29401 aaccagctaa caatgcacca atgaatgccc atggtctggt catcgtagcg actgcaaaca
    29461 ttcccgatgc cggaatacgg ctatctgcgc cccaaaacat gctcaatcca ccgccgactg
    29521 ccccaccgac tcccgttgct atgacattgc gtgccaagtc gttcaatacg atagggatac
    29581 atccctctgt gatctgacag atccccatag gaaaagcggt cttcaatgta tcgatttctt
    29641 gacgattgaa tatattttta cgaatgattt tccctaagaa ataagcgatc gtcaagccga
    29701 atggggttac catcgaagct aaaatcaata cagtgattgg ttcatagatt ccttctgaaa
    29761 atcgtgccac acccccttat ccagaataaa tggtaatcca gaaacagccg ggtatctgcg
    29821 gtaataagcc taaccaaacg aaaaatatga gaagctaata tgagaagcta tttttgtttg
    29881 gctgttctta ttacttgatg cagggcagct ttttcacaac ctcttttttt atttatgttt
    29941 cataatcaat atggtaagga tgcagctccg tatggtagac cgcctccgaa tattcaatca
    30001 tttccccaat ttgcgtagac gatttccgta tccgtttgat gccgatctgt gcgtctgttt
    30061 tcagaatttc ttgttcttct tctgttaaaa atactgccga gaaatcatca gaaaatgagt
    30121 caatagtata tccttttttc gtcagcaaac gatacaaaga ttctttgcca aattcttctc
    30181 tgctgaattg catcatttct tgtggaagat aatgaacaaa ataaatataa ggctgattgt
    30241 tcaaataata taagcgctta aaacaaacag cttgctcacc gaaaaaggaa aacaaaggat
    30301 cttccttctt tatatggacc aattgcaaat ccagcgtttc tttccggact tcatatcggg
    30361 agttctccaa gatttgcgta aagcttgccg ctttggataa tcgattgtaa ggacgattgc
    30421 ttagaaccgt cgtcccacga ccacttttct ttgcgacata ttcatctgat gccagcagtt
    30481 cgattgcctt tcggacagtg attttgctga cttgaaacag ttcttctaat tcactttctg
    30541 tcggtaacat cgaaccgact ggatattttc ctgagaaaat atcttcttta attttatctg
    30601 cgacttccaa gtatagtaca cgtgtccttt tcataacgtc accctttgct ttactattac
    30661 taaaagaata gcacataaaa aataaaaaaa gaacacaaat cacatgtgag aagtgtgttc
    30721 tttttcattc agctgcttta attacaagtt tacgccattt gaggcaatta cttctttata
    30781 ccaatcaaaa gattttttct ttgatcgatt cagtgtccca tttccttcat cgtctagatc
    30841 cacatagata aagccgtaac gtttactcat ttctcctgtt gatgcactga caagatccat
    30901 acatccccaa ggcgtatagc ccatcaaatc tacaccgtct gctactgcat tagccatcgc
    30961 ttcgatatga cgacgtaaat aatcgatccg ataatcatca ataatcgtgc catcctcttc
    31021 catatgatca atggctccaa gaccgttttc aacaatgaat aatggtttct ggtaacgatt
    31081 gtataattca ttcaatgcga ttctcagccc ttctggatcg atctgccagc cccattcact
    31141 tgcctcaaga aacggatttt ttactcctcc cagcaagttc ccttctgaag ttgctgcttg
    31201 tggatcagtc tcattgattg ccgtagacat ataataacta aatccgatgt agtccactgg
    31261 atattctttt agcaactgga agtctttttc cgtttgctct aatttttccg gatcgactcc
    31321 atatttttta tgggtacgcg ctgtgtatgc agggtattct ccacgtactt ggacatccgt
    31381 gcagaaaaaa ttgaattctt ggttttggat cattgttgct gcctgattga ctggattggc
    31441 atcgatgctg taagttgttg catagataat catgcagccg atttggatat ccgaacgcaa
    31501 ttcgtggccg atttttacgg ctttgctact tgcaacgaat tgattatgcc atgcttggaa
    31561 aatattttgg tactctcctg ctccattgct ttttactaat ccttgactta aagctggaaa
    31621 gtgaaaggcc gaattgattt cattgaaagt catccaatat ttgactttac tgctgtatct
    31681 ctctaatact gtctgagcaa atcgttcata gaagtcgatc agttttcgat ttttccagcc
    31741 accgtattct tttgccaaat gaagaggcat ttcgtaatga gaaatcgtga tcacaggttc
    31801 aatatcatat tttaagcact catcaatcag ctgatcataa aattctaatc ccgcttcgtt
    31861 aggagtacct tcgtctccat ttggaaaaat tcgtgtccag gcaatcgaaa aacgataaca
    31921 tttgaatccc atttccgcaa acaaggcaag atcttctttg aaacgatcgt aatgatcaat
    31981 tcctcgatga ttcgggtaaa tatatttttc ttgatcaatc gtccagtcaa attcttcact
    32041 gccgataatc gcaaagcgct gcttacctcc aggcatagca tctgcaacgg atagcccttt
    32101 tccgtctcga tcataagccc cttctaattg attggcagca gttgctccac cccataaaaa
    32161 tttttctgaa aaacgtgatt gcatcctgat ttcctcctta ttttacaatt gccagaacat
    32221 cttcgtttgc aataatctct ttttggtcta attcaagtat atcggcaaat gcatcagtat
    32281 tcgtaatgac aactggagtg attacgctat agccagctgc tctaattgct tcgatgtcaa
    32341 aacgaatcaa taaatctcct gctttgacga gatccccttg tttgactaag atctcaaagc
    32401 cattcccatt taattctact gtatccatcc cgatatgaat caaaatctcc gctccgtcag
    32461 tcgttgtgat ccctacagca tgtccagttg gaaaaatagt cgtgatctct ccgtctgcag
    32521 gtgcaactag ttctcctact tctggatcaa tagcgatacc ttttcccatc acgccagaag
    32581 aaaaaacttg gtctggtact tcactcaatg gcagtacttt acccgttaaa ggacttttta
    32641 aggtatgccg tgctgaagta atcggttctc ctgcattcgc atgtttgttt tctaattgat
    32701 tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc tacgaatgca agaacaaaag
    32761 caattcctgt agctattact gctactgtca catttgactc tacaccatcc accgtattaa
    32821 tgaaagtcgg aatcgtcaat aagctgacca aactgctgga gaaagctttt actccggaaa
    32881 atccgataat agctccacca atacctccag aaatacaagc ggcaatgaat ggtttcttca
    32941 aaggtaatgt cacaccatac acagttggtt cagtgattcc aaataacgaa gtcactgtag
    33001 aagaaatccc taatgctcga agtttcgtat cttttgttcg caatgctaca gctaaagcag
    33061 ccccaccttg tgccaagatg gctggaagaa gcattggcat caatacatca aacccatatt
    33121 gttcgatatt caaaaacatg atcggtacga atccccaatg catgccgaac atcacgaata
    33181 cttgccataa acttcccatg attagacctg ctacgattgg actaaatcca taaatcgagt
    33241 tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat tggtcctaaa gcaagaaatg
    33301 ttaatggaac catcactact aaaacgacaa gcggaactaa aatcatttgc agaaattgcg
    33361 gaatcacttt ttttacgaat ggctctaatt tactttgaac ccatacggct agaatgattg
    33421 gtatgacaga agaagtataa ccagatggtg aaagaatcac tgggatccca aagaaactga
    33481 tagcttctcc cgcaccagca actgctgtga tagctggata cacaagtgcc atagcaattg
    33541 ctactgctaa aaactggttg gctttgaact ttttagctgc tgtaaaagca agcatgacag
    33601 gtaagaaggt aaagacacca tctgctgcag caaacaatat ttgataagta ccgctatcag
    33661 ctgtcagcca gcccatcacc gttgccaatg ataagaatcc tttcaatacc ccggcggctg
    33721 ccatcgcccc tagaaaaggt gtaaatatac tagagataat atcgatcaga cgattcaaaa
    33781 tatttttgga ctcttctttt ccatcctccg aaactgcaag accttcctca tccacgatcg
    33841 attgataaac atctgctaca ttactgccaa tcactacttg atactgaccg ccactttgta
    33901 cgacttggat cacgtccggg tcttctttta acttttgcgt atctgctaaa gattcatctt
    33961 ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact tttgacattc ttttgtcctc
    34021 ctaccgcttc ccatacacga cgaccaactg cttgattatc cattgttttt tccttctttc
    34081 ttttttattt aaaaaataaa aacccagatt gaaaaaaaca gtcacaaaca ttcgtgaacc
    34141 tattttttca atccaggttt tgcctgccat acagtaacaa tccttatgat tggatgcttt
    34201 ttttctcact tactactctt tgaatatgaa tcgttaaata aatctgctct gcgatcccca
    34261 tttcctgttt ttgcgtttgt cgaagatact cattgatccg ttcgacacaa tgaaacgctt
    34321 ttggatactt tccttgaact aatgcgtaga gaaattcatc ttgctcgtca gatccttccc
    34381 ctttgagata acgctgggca aaaaactgca gatgagtaat gatacgctga taattcaaag
    34441 attcttcatc gaacacgatg ccgaagaatt tactgatgat actcagtata tctcttacca
    34501 ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc ttgttggcta ttcacaaaat
    34561 gaaaggcaat aaaaccggct tcatcctcag aaagttccac acccgtcttt tgtttcacga
    34621 acaaaagagc ctctttcgct gcttgatatt ctttcggata aaattttttt gtttcaaata
    34681 ataatggatt aggcagttgg atattttctt ttgctcgtgt gatcgcataa tgaagatgat
    34741 cggtcaatgt cagataaata ttaggcgaaa attcaacttt tagtttatct tcagccagtt
    34801 cgactacttg tttgacccat tcgatctcct ctgccggaat atcagccatc agctgctgga
    34861 attggttttt agcaaaagaa tctcttaaaa caaattcttt ttggaccgct tctggcgaaa
    34921 tctcgtcccc aatctttttt tgaaaagcta gtccttttcc catacaaacg acttcttccc
    34981 cgaatccatt tttcgaaatg acgacattgt tattcaaaat tttatcaatt ttcattttgc
    35041 ttcgccctct caagaaaaat aaaaaaacct agaattcgcc tactgtttct tagctgaatt
    35101 ctggttttgc ctgccgtaca gtaacaatcc atttcttatt caaaatcatc ataccatcga
    35161 atttttattt atgcaagcct tttcatataa aaaataaaaa aactggaaca caagtctatc
    35221 tagaaaacaa ccaaatcaga gatgcacctc tttgcgtttc tctttttttc agcaaattgg
    35281 tttttcctta tcgattcata tcctttggct gtttttaatc tgtaacgact tatgtcccag
    35341 tatcctaaaa tgtttttgtc tctagattga aagcagatct catatcatct gtcgcatctt
    35401 ttatcgatgt aacactttcc agttctcctt gtacaactag tcgcgtgacg gcgtccatgt
    35461 cgccacaagt aattagtgtg acaatttctt ttccctcgac ttcattcagt aattgggtat
    35521 ctgtaggagc aactttttct ttaaaaaatg ttttgtatgt atacacattt tctaaatctg
    35581 tcaaatagat cttggcaccc atctctaaat tttctagcgg tgtaaacagc aattcagggt
    35641 tcgttgcacg atggcttgct aaggcataat ttccttttcc catttcttga tctggagata
    35701 atgttcctgc tccgtaaagt agtgacgtat tatccaagcc tttgaaaacc ggtaaattga
    35761 ttgaaactga agggactgcg atactagcaa tcactggtag atctgttcct ctcatttgcg
    35821 acctcatgac cccttctgtc gtcattggtt ctaccgcatc aaagtcaaaa ctgacatctt
    35881 tatcgttatt ctttttcaga tcttcttttg ttacattcgc tatagcatat ttatctcctg
    35941 tctctctgac aaaataatcc ttgatctgtt cattgaaaat caaagctagt cctactaata
    36001 acaagaggaa caagaggata ttgactagcc atctttttgc ttttcctttt tttttcattt
    36061 tctctccctc cagactcatt ttactgtttt tcttttgctc tttccaacag ccgaatgaca
    36121 catttctaca actctgtgta tttttttcgt acacctgcgc ttttttctac aggatatttc
    36181 aaattatttt tgatcaattt ttcttcaatg atctcatcta gatccaggtg aaggttgtct
    36241 gccatcatgt atgaatagat caatacatct gccagttctt ctttgattcg ttctaattgt
    36301 gtttctttta cttcttccgg cgttttccat tgaaaaagtt ctaacaattc agatgcttcc
    36361 aaagaaatag agatagccaa atctttttcg ttatgaaatt gtcgccaatt acgttcatct
    36421 ctaaactggt tgactttttc catactattc atttgcttgc cttcttccaa tcacttctta
    36481 cttgattaat cataccattt ttttacataa aaaatagatg atgcttttaa aaaaactcct
    36541 gttttctttt atttaaaata aatgtcatag aaaatcaata atttaggtag ccaccatgtg
    36601 tatgcagtag cgtgtcttct tcccttgagg ctttttgttt ttcgatacgt tcttcttccc
    36661 aaacagacaa cgctactaga atctctctta atgattttcc ttcttcggtg agcgagtact
    36721 ctactttagg cgggacttgg ttataggctt ttcgatggat actttcatct tgttctaact
    36781 ctcttaactg ctgggtcagc attttttttg tgatcgtagg gatttgcttg cttctccacg
    36841 cctaaaataa gcgatattgc tcttcccttt atctggtgta agcaaagcag ttagcatttt
    36901 gacgatcgtg ggttccgctt ttcacttctt atctacttag tatcatgaag tatactttcg
    36961 tactaatact aagtagcgga agtgtcaact actttgttta actacatagc aaaagaaaca
    37021 gaagggtgac aacggtcatc acgcttctgt ttcttttgaa tatgctgtgt tgttgtctct
    37081 atctccgtgt agactttcct caaacttatc ctttgacagt aagtcagtag gctgatttgc
    37141 tctttctaga tgccagatac atttctacaa aaaaatagtg attctgtttt gaaaattcga
    37201 tttttagttc tgcattttcg taaacatcca acattttttt cgcactgtat agacctaacc
    37261 cgccattttt tttagaggtt tttcctcttt tgaccatctc cgatagcgtc aaattcgttg
    37321 gatttgcctt gttcgctata ctaaaacgtg atccctcttt ttcttccagc agattgatcg
    37381 taatcggact ctgatcggct acacgatgat cgaatgcgtt agaaataaaa atagataaac
    37441 agcgaataaa aacaaccaga tcaatcggga ttgttttcac ttcgacagga ataactactt
    37501 ggatcgggat tttctccgat tctgcttttt ctagaaattt taaaaagaca cccctgacag
    37561 ctggggatac aatatttgtt agctgttgta tttgcggttt ttcaaaaata gctgcagaat
    37621 gatcttctaa tatttctaaa aattccaacg tttctttttt attttcctga agagtaattt
    37681 ttaatgaaaa cagtagactt ttataatcat gtttgaattc ctctagatga tggtaatatt
    37741 cttcatttat ttgatacagt tgaagcgatt cctgatgttg tctttctttt acttggaaac
    37801 tgatcaacat aatggaaaaa aacaaagaaa aaaaactgat gactatcgac aaaagagata
    37861 gaagcagata attagatgag cggaagtgaa cacttcccaa taccaagtaa atcacgcccc
    37921 agcttaataa aatacaaata cctccaaaat agtgttcagt tggataagta aaaagcaagt
    37981 tgagaaatct cgtctgtttg tctatacttt ttactaatag atagaaaata aatgcgataa
    38041 gtagtgcaaa aaaacttgct gttccaagat gtctctttcc taaataatcc atcagatcaa
    38101 aaaccagaaa aaagctgagt ccagtgatag caaaaaaaag attactgatc aaacagctga
    38161 taaacaggtt tttcgtagta aaataaaaaa gaaacatcaa taataagcca attaggtaag
    38221 gaaagctcac tttagggata ggaagtaaaa gtgtcaccac catcaaagct atacaaccaa
    38281 agctgttttt tactagcagt tgtttcgaaa agaataaata gatacctaaa tctaaaatca
    38341 aagtaaaggt caaataaagt gtcactgttg cgaccatctt attcctccat tgttttgttt
    38401 caaaatgtac acatattttt ttaataggag cctgcatatg aaaaagtatt ttatcataga
    38461 agataaccag tttcacagcg aatggatcat cagagaattg gtcaatcatc atatcgagaa
    38521 tatcgtgcag ctcacttcta tctctcaaat caatcagttg atccagttgg gcactattga
    38581 taatcacgat gttttcttta tcgatatcga cttgaagcaa tcactcacag gtattgatgt
    38641 cgccgaacaa atacgtaaaa aaaatgcaca tagtaatatt gtttttttca ccgcttatac
    38701 gaattttgca gtagagatca tcaatcggaa aatccttccg attggatata tcagaaaaga
    38761 caggaattta tctgaacaaa ttcgtcaagt gctagaacag atcaattgga aagaacagca
    38821 gatcgtaaat gtcgataaaa tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc
    38881 tcgggaaatt ttatatattg aaagtatcaa aggtttgaaa aaccaagtta tggtcaaaac
    38941 aactacagaa gataaattag tgaatacttc tttaaaacaa ttgaagacag agttagaaaa
    39001 gtatgcttac tttttacttt taaaatctta ctgtatcaac ttatcgcaat tgcagaaaat
    39061 tgattctgct cattatgtgt tagaattttt caacggtgac tccttactcg ttggtcgaaa
    39121 gatctttgaa aagacaaagg aacgtttcca tgacttccaa aaaactgctt cgagttgatg
    39181 aataagagct gagacgtaag catagcagat ttcagggagt gggccttgaa tcggctccac
    39241 tccctgaagt cgttgatatg attttacgct tcctcttctg taggagcatc tgttaacgac
    39301 catgtgaatg ttgaaacgta gttgtctaca gcataattgg atacaggaac aaataattcc
    39361 actgattgtc tcccttgttc cacattctct cctaatgaat aaatccatgt gccaatccct
    39421 tcatttgctc ctgcagttac tagatctact gcctgatctg ctgggatttc gattccttca
    39481 gcgccagttc cagaaacggt agatggcatt ccactgctat tagttgcgct atttcgaatc
    39541 acggcgtttt tgaatttcac ctttgaacca gttagtttat tcgtatcaga tgttgtacca
    39601 ataaattctg ttcgtttgac tgataatttc caaccggcat aatttccgct tttatccgtt
    39661 acttgtacga aattctgtac ttgtgtttgt gctccagtac tgtccgttcc gctttgtaat
    39721 gcagcatata ctgtttttgc tgtacctaac ttgattgttc caaaatcaag atctggagct
    39781 tggtcaatcg tcagtaatcc ggatgttccc tgatttggtt cgtcaggtgt aaaaggagta
    39841 tcaggatcat ttggatcaac aggaggaact ggaacaacgt cttcaggatt tgcagcatct
    39901 ggtttggtca cagaaatcgt tccttctgta gtggctgagc tcttattctc agcaaaaaca
    39961 ctttgtgtac ataataatag acttccagct agaacggtac ttaatgcaat tgtttttttc
    40021 atttcttttt ccttctttct tttaaaataa ataatccagc aaataagcca ataagaatga
    40081 ctccaacaag tatcagccat cgccagtcaa atgtttctgg ctgatttttt aaatcaatat
    40141 ccttttcatt caattggtcc gctgcatcat cagttatttc aaaatcggta tggagattcc
    40201 agcgtttttc tttccaagca cccttcacaa ccgcttgata atgtcccgga gctaattttt
    40261 ttccttctaa tgggacaagt aaatcaaaat ttgtattggg tgctacatct attttttctt
    40321 tttttattga aaggtaaggc tcttttgttt cttttttgta gatttcaacc gatgtagtca
    40381 agccgaacat ataagaagca tttggatttt gaagattcgc cttaatcaca tttcttccat
    40441 cgctttgatc cgcataagct ttttttaatt tcaaatctgg ggaagactct ttttcgctat
    40501 ttcaaatcaa gattgctttc acttgagaaa actgattgac gacaccaact gtacttttgt
    40561 tagaaacgtt ctcttttttt tcttgaaacg tgatccctcc tgcgaccaca ccagccagct
    40621 ctttttctgg cgctttcagt gaaagtgtct cagtgaccgc actttgagga ggaacagtta
    40681 tttctcccac tgtttttgct aagtttttca tatcataact taatgtttca tcaaatttac
    40741 tttttgattc tccatattcg atcacaccgt ttgaattcgt cgtagcggag tggatcaatg
    40801 tctcaactgt tactgattta tctgttcggt taaagaccgt gtaggggatt tcctgtgttt
    40861 tcccaggctc catccttaca tttatatatg ttttgttctt atcaatctga ttagccggta
    40921 attcaaaatc taccgcaaaa ttgaattcgg aggcatggac aggtgtctgc caaaggatga
    40981 atccaaggag aaggatcata agtaaagagc agttgtttcg atgactttta ttccatattc
    41041 gattcatcaa tcatcctcct tgatctttat tctgtcggcg catctgctaa tgtctatgtt
    41101 atctctgctg agtaattacc agctatctga ttttctttgg ttaagacaag tttaaacgcc
    41161 cgaccgttat ttaaatattc tgaataatct gcttgcacat tcgttgaatt ttgtcctctg
    41221 acaatttcca gttcttcttc attcactaat tgatactcct ctcctttctt gaaatatagg
    41281 acatcctgca tagaaaatcc tgcatcattt gtgaaaggct gagataattt tatcgttaag
    41341 atccacggat ttttcgtttc tcgctgatca taaatccata atttttcctg tatatccatt
    41401 tggccaaatg cttcttgtgt caaaccagtt gctttgatct gaccaaaatc gagtttggaa
    41461 ggggcaagaa atgagagacc accttctact acgatcttcg tagcaggtgc aaaaggcggg
    41521 gcatcatgat aaacaactgg cgtatcactt ggattgtgat tcccaatttc atcgttggta
    41581 cttggcttgt tctgcacacc cttagcagta gcttctcctg cttggtcgtt taatacaatt
    41641 tctaatacat ctcctgcaaa aagttgattt tctgggatag aaagaacaaa ttcccctgta
    41701 tcatcaatag ttgtttctga caaaacagct tgattttttt tcaatgaaac caaggctcct
    41761 gcttcacctg tacctgtgat cgtgcgagaa gctgcattga tcaatgtggt acttaaatta
    41821 gcaggttcag gaggaatcac atcgactgtc gttttggcag agtccagttc tgtcacttct
    41881 tggccacctc ttactttttt agcagcaaca acttgcactt gatcctccgt ttttaatctc
    41941 tcatcattgg gtacttgaac ttcgaacaag ccgccttgtt cgccttctcc ccatgggtta
    42001 tatcctgccc cttcattttc atctgtttgt ccttgcgtga gagcagtaat ttctacagat
    42061 gatttcgtcg tatctgcgta agtaatacgt aaagtcactt ctacttcgcc tgtccaagca
    42121 tctcttggct ttgcctcgac accttcggga acaacgacat gcccatagat ttttttatct
    42181 gcattcgttg gcactcttaa ggattcaatc atcgggagct gattattggc actgattcga
    42241 ttataatttt tcattccggc acctgtctga gcaaattcat ttgttaaacc tgcactaggg
    42301 cttgatacga aattgcctaa gtcggtccca ttgatcgata agtctgtcat atcagaaaaa
    42361 taatccgggt ctttttcaaa gttatcgaat gttgcgcctg tttttcgcca taaagcgatt
    42421 tctgaatggg cgatctttaa agaagaatct ccatttcctt ggaataccca gccgcctatg
    42481 ccattggtct cgcttccaat ccgtttgttt tggaaatcaa aataggtagg agattctaag
    42541 tccacattca acacatcccc actgatgatg ccggtcttat cttcccctgt tttcccgacc
    42601 atgactaaat aagttttttc acctgccgta aaatttaaat caatatctcc agttgtatct
    42661 acagttgctc cggaatctgc gcgtatatcg atatttgatt tttctccagt gactgtaaaa
    42721 tcagctgaac ctggactttc tagaaaacca gaagcgtcat aaaattggat cgcttgattt
    42781 ccttgattga gtctgttcac gtaagatttg ccatcacctg tatttcttac cctcaatgag
    42841 cctccattgg ttactgtcag cttttgcgta ccattctcaa aacgaactgc gctggaacgc
    42901 ccactctgct tatcgatgtc gataactgat ccggctcctt ctacgatcaa agaaaggcct
    42961 tttcctacaa aacgcatccc attgttcccg ggttgatcgt catctccagc cattttgacc
    43021 gagatttgtc caccacttga tacatggatt tgactagaat ctccttgcaa gcgaaatccg
    43081 cccgtagttg ctgtattgat ggtgattttc ccaccattcg atacagtgac tttcgatcct
    43141 gaacctctca tttggatgat tccgcgattg ttgtcattgg taatattgat ctttgtattt
    43201 accgaaaatt cgcttcccga accactgact tcaaacatga cattaccagg tagcgtatcg
    43261 actgcctctc cttcatcgct aacgccatct gcccgatcat aatcaagaga gacctttgaa
    43321 ccgcttaaga tatggattcc tggcgctgtt tttccactac cagtctcatc tcctgaatct
    43381 agaaaaccgt agaatttccc tagatacggt ccataaagtt gactaccatt gatcacattc
    43441 aattgatagc ttctacaaaa ataggcttta cccggagcgg cattcagtcc ttccttcact
    43501 ggttggacat ccaacacacc ggaaacacca tctaatgtga cagttgcacg aggagcgtaa
    43561 atcagtccta atttggaccc atcatacaaa tcaagtgtcc cggtaaattt tatttctccc
    43621 gtaaaatgat atctgcttcc ccaaaaatcg gtcatataag caatgataat ggcatttcca
    43681 tcgatatttg gtgtcaatcc acctggatgt gcagctgttc ctttaaaatt ctggaactcg
    43741 atcgactgag gtatctcatt gatataaata tggtgtgtgc cgaaattgtg agcgaatcca
    43801 ccaaaatcaa taacgatatc tcgtttgatc tctaattccc tgtctaaagt caaaggctga
    43861 atcagtcgga tcttagttac tgattcgtct gcgagaagct gcgccagttc ttctgttgtg
    43921 gcagcttcgc tgtacccatc cgctgaaaca gctcttgcca gtgccgcttc actgctatca
    43981 gacgtttgtt cagctacttc tgaggtctca cttgtcttat tttcttcttg ccttttatta
    44041 gtagaagcct cactttgaga ttctgtagtt tcagaagtaa tcgattcttc tgaaaaagta
    44101 gtcttgtcaa aactttgatt ctctgattgc gttgattcgt ttgttttctc tacatcggca
    44161 aaaacggatg tcaccatcaa gagagggaag acaaacaaaa tcgacgcaat cagctttttt
    44221 acacattgat tcattctcca ccatcctctc tgacgttttg acaattttat tttatcgttt
    44281 taatctattt ccaattgtac tttcatattt aaaatatgta tttttatgga aaaatttttc
    44341 tattaggtgt aaatttcttt tttgtacaaa aaaaaggctg tgacaaaagt cttgtcacag
    44401 tcccttatcc cgaacaagcg gtgttctccc gccaactcct cggtcacaac ctccatttta
    44461 acaataaacc tgtaaatgtg agggcaatac tttgattgtc acaggtaatt tatccccttc
    44521 atctccatca acattcgtcg tcaattcttc ctcatcagct aatgacacaa ctaattgctc
    44581 acaagtaagg taggctagat ttttcgtaga ttcatctact ccttttaata ggtctggcaa
    44641 tgttttaagc gtatcgatca aagaagaatc ttttaaataa accaaatgga gcttaccatc
    44701 atccactttt gcatttggaa gtaatgtttc aaaaccgccg actgagttag ttagtcctat
    44761 cagcaaggta ctgctctcaa tttcttcctt ctttccatca acttctacgt gaaacgagta
    44821 ggattgtgtg gaagctaact gtttgattcc tgacatgaaa taagctagtt ttccaaattt
    44881 cgtcttcttc tccgcatcta catcattgat tgattcaggg atagagccaa ttgcgacgac
    44941 attcataaaa tagtcgtcat ttatttttcc aatatccaat ggcttgactg attcaatcga
    45001 aaaatgattg atcgcttcct caggttctag cggtatccct aatgctcgag ccaagtcatt
    45061 caccgtaccc aatgggaaaa atccgaaatt tggccgatgt tcttgttcag cgattccact
    45121 gattccttcg ttgactgttc catctccgcc catgacaaac acactatgat acccttccgt
    45181 agccgcttcg cgagtaaagt tttttgcatc ccctgctttt tttgtatgca agacgaccac
    45241 ttcatcaaac accgattcca attttgctat cgctaattgc tcaaattcct ttgcttgttc
    45301 accgcctgaa ctggggttta ccactaataa aacttttttc atcactttct ttcccttctg
    45361 attgatttct ttaatataac gatagcataa agaaaaaaat taggctgtga tgaagtactg
    45421 ttcatgaaac attcatttat tttttgacaa gataaaacat ccatactatg aaactgagga
    45481 aaattaaaac aatgatatag gaaataaaag aaaatgatag gcattgtata cctaataaag
    45541 taaaaatctt aggtggcaga taaaaacaca aaaatagcaa gagaatgtaa tagcgtttct
    45601 tccacttttt gttatcagac cttatagcga tcagaggaat accaaatgca taactgaaaa
    45661 actcttgatg tactgttaat atgtcacggc cttgaataat aaatgcgaca atccccgcta
    45721 taattagtgt gcctaacacc aaaacggaaa attgatgaat atattttata tacaaatgat
    45781 ccattgtact tcctgccttt ttatttgtga gaaaaacaaa tccgtatacg tgaataactt
    45841 ctagaaataa aagaaatttg caaaaccact ccatttatct acttctacgt gcattttttg
    45901 atattgtttt ctttatttaa gattatacag aaaaatatct agggtcgctg gattctaagt
    45961 aaaaaaaatt tttaaaataa aaagaactaa atcaagtagt aattaaacta ctgttgattt
    46021 agttctaata aaaagattta gatgaacagt tttattgatt taaatagttc atatttttta
    46081 tttataccgc ttatttacta cttgctgtac tagaatctga agtgaatgta ctcaaaacat
    46141 tttcaaagtc tttgtctttg atttttacat tagcgtcttt caatacttcg ccaattactt
    46201 tattctggaa ggtactgtca cttaattttg tgtctgttgc aatttctttc aattcttttt
    46261 tgtatttatc catgtcgttg cctttgtttt ggtttttcac cattttaacc acataatatt
    46321 ccgtagtata agttgaagca ttggtagaag tgatcacatc agaaacttga ccatctttca
    46381 acttaaaggc tgcttctttg acttctgccg gaaccgttgt agtagtggaa tcaaatttca
    46441 cttttccgcc atcttctttt gtcgtagtat cagttgactt gtctttggct aatttagaga
    46501 aatcgtctcc cttatcagca gctttcttca catcttttgc atcatcttca cttgctactt
    46561 ggataatttg agcttccact tctggatgga aagattccca agccgttttt aaatcttcat
    46621 cggtcaattt gatatgtttc tttagacctt cttgaaaggc tagactttgt ttgatactgt
    46681 ctttgaatgt ttgttctgtg taacctgcag atttcaattg tgaatcaaat gaatcaccca
    46741 attgtttctt tgtttgatca tattgagaat cgatttcttt ggttgatacg tcatctccat
    46801 atttatcttc aaatacttga taaacgatca actgttgcac agcggaatct gctccttgca
    46861 tttttttcaa gctatccatt acttcttgtt cgctgattgt accgccttta taactaacgg
    46921 ctgttttttc accggcattc gtacagcctg acaataagcc agtgcctagt acgatggcaa
    46981 gtgctaaaaa tctttttttc atttatcaat tctcctactt ttcgtttttt gcgaattgct
    47041 aaaaacaaag gtgttcaaga tgaaaatcat caagaacacc tttactaatc tgctcgatac
    47101 gtcctgaata gatataaggc tacacaatcc agaaagttcg ctcagcagaa taaatgtcaa
    47161 gtagcacatc acaccttaat atactacttc ttttgttacc gtttttcaag tctatcttgc
    47221 gttccgtatt taacgtaact aattcttaga acaaatgtta tcgattttgt ttttcttctt
    47281 ccgctgctgg gatattcaaa tcagacactt ttatgttcga ttttccagac atttttttgt
    47341 tgtttttatt atgatatatt tttatacaac taaaaatgaa tatattaaaa ctaccaaaaa
    47401 taacttttaa cgctgtttca ttatatgttt tttctatttt atataaactt ttggatactt
    47461 aataccaacc ggttggattg taccaacatg agaatgtgtc agttctattt cctgtcgcac
    47521 ttgcataggc ccatactcct tttgttgcat ttgctgatcg aatagagttt ccaactttcg
    47581 cttttgcacc gtgacgatta tagacacgac tatagtggtt agaatgacct gtttttctcg
    47641 tacaagttgt atcactataa taaacccatc ttccagagct atgattaatt gctgttctag
    47701 aaatttcccg gtctactggt aaatttgact taataatatt acctttagca tcaaaatgaa
    47761 tctctcctgg ctttaaagat ttgtaataga tttcaccgtg aactgaattt gcttccactt
    47821 cagatgacat agcgtttcct ccaacaaaac ctatccctag tgcacaaata ataactgctt
    47881 ttttcataaa acgacatcct ttcctttttg atttttaaag catgatcttc ttgtttccta
    47941 ttttggtcag atatggatca tgcgtgacta cgataactaa cgtaccttgg gaagcttgtt
    48001 gcgataagat ttcaactact ttatccttat ttcttttatc caatgaccca gtcggttcat
    48061 cggctaaaat gattttgcta ttcttcaata agactctcgc acaggcgatt ctttgcttct
    48121 caccaccaga taatgtgtag ataggcgtac ttagcttctt gtttatattt acttttttca
    48181 atgcctcata ctttgctgtt cgcttatctg aaacatttaa attcgaaaac ttcaatccaa
    48241 tatctaaatt aaaatcgatt gtttcatatt caatcaaccc aaaatcttga aaaagaaaag
    48301 atatggtatt tctttttatc aagcgcttct ctctcccagt taattgtttc gtctcttgat
    48361 cgaacaaata gaattgtcct gaactgaact gatcattcaa tcctaatata ttcaataacg
    48421 tacttttacc tgctccactt tctccaacaa cagtcacaag atccccagca tccgcagtga
    48481 aacgatccaa atgaagaatc gttttgtttc gaatgatttt tttcgcacct tttatcacta
    48541 tcaacggctc caattgttaa cctccattca tcagcaataa ctgacttttc ttttctgtag
    48601 acaaaataaa aacaactgaa agaataaaat caatgaagct aaagaaaaaa atattaaaaa
    48661 gatatttagg ctctctttct ataagaaaaa ggaagattac acttgtcgtt ccccatttga
    48721 tgaggttcat catgaagaaa tctttatacg tttcaaagag cgaataacca cttgaacgaa
    48781 agatagcgat tttccgacaa ttgtagacaa aatatatgca taacatgaca tagctcaaca
    48841 agaagaaaga aaagaaagtg agtaaaaata tctgtatcaa gtaatgaaat gatctgctta
    48901 acactttgac agctatctct tcagtctgag tatacggaaa tgataactgc aagttatcac
    48961 gtaactgact ttcttcaatc agctcaaata acctttcttt cgacaaagag cctatcttga
    49021 tctttaacgg tggatagatt tctccgctga gaatatttct ttccaaatac atactgtttt
    49081 tcaatgtcag tatttcaaca accggataat gttcgatcca ggggatagaa ggaataaatg
    49141 tataaatggg ttgattgttt tttataaata gaaatttcgg ttctttttca ctgatttctt
    49201 gataatattc tgttatttgc gccaggtcat tttttaaatc tgtatgtcta tatcgttcag
    49261 ggatcaatat aacagggtgc gtttcttttt cggttatttg gatcaacctt ccagagacat
    49321 caatcaaagg aaatttctta agataattca gatttatttt tatccctctg ccaaactgtt
    49381 cattttcttc attcaagtaa tctgatacat tgactaatat acttccgttt ttattcaaag
    49441 tttcatataa ttcctgatct gctgctgatg caaaacgatt ggaataaata aaatccacgt
    49501 ggtttttccc gatggttact ggataaaata catgatagtc ctcaccagcg atttcttttg
    49561 gtggttcatg ggaaacagct aactgtgaag aagaaaaaac gatagttgct aattcgacat
    49621 tggcaacggt caaaagaaga gaaaacaaca atccaaatgt aaaaagaatg tattggatat
    49681 agttgaacgc ttctcttaac gaagcgattt tttcaactaa taaaagagaa atcaaagaga
    49741 taccatatat tccgctgaac aagaaaacgt acatctggaa caaataaaga aattgctcct
    49801 tataatacat aaaaataaaa ccaccaatga ttcctgcaat tatgaatgta ataatgattt
    49861 tctttttccc caataaacac cacaatactt gatgtaatga aaagtcatta ttcaacaata
    49921 aactgatttc tttattcatc aataagaaaa aggatgataa caaaaggaat aaaagaatca
    49981 atccaatagt taaatacact cgataattat atacgtctgt atttgaaata accgaatacg
    50041 aatagctatt ttcaaagtct tcaaaaatat tagtggcata atcagtgtga aatgcttgat
    50101 tgaatcgatc agtgaatagt gaaatgaccc gttcataggc ttctttttct ctcgtcatca
    50161 agaaaaattc accttcaaaa tcttttattt tgatcgattc ttcaatggac gttacgcgaa
    50221 aggaggtatt agaatagggt accttctcgt gagaagattt gacatcccct tgataataca
    50281 atgtcatttc aagctttggc tgtatcttga agttccaaga tgtatctact tcattggatt
    50341 catttactgt ttttttcata taagaaagct cagtttctga tgagacattt tcaaatacca
    50401 tgattagctg ctcatattct tcttgcccaa taaaatgttg tggcaatcga atcggagtat
    50461 aagtatctgt ttgatggaaa aattcatttg tccgatagag tacctgttta tttatactaa
    50521 ccaaggcaaa accactcatg ataccggtca ctatgattgt gagtaaaact gtgaactttt
    50581 ttttgattat caccactctc ccttacttaa atgattcttt gataaaatca taccgttttt
    50641 catcaaaaaa acgtatctat caagtgtata ggtcatgtag aataaatcta tatactgtag
    50701 aatcgaaagg agaagaagat gaacaccgga gaaaaaatac gaacttaccg cagaaattgt
    50761 aagcttacgc aaaaagaatt aggcgaaaaa attggtgttt ccgataaaac gatttctagt
    50821 tgggaaaatt ctcgtacaat gccagacttt gaaatgctgt cacttttaca tcatacttta
    50881 ggtctgccga ttgattttcc ttctattgaa tcagctaccg aatcaataga accaacattg
    50941 ccagatgaac ctcttgttaa caagaaatct tattcatcaa aacaaccctt ctcactcaaa
    51001 aagacactat ctttctttct gctcgtcttt tgtttggtct atctttttct tatcaattat
    51061 caatacaaaa atgaaacgat cgatcgattc aatccttttt tgaaaccaca aatcggctat
    51121 accacactcc ctgtaggata cgtggacaat tccatcgaac gttgggtatg taatgatatc
    51181 tttggccatg gtacatggct gtcttttcac gctggagagg tagaaaaaga gaaaaaccat
    51241 gctgtaatca aacacaaagg aaaacacgtc atgaacgttc gcctgctcac ttgggaagcc
    51301 attccctcag attaccaaga aatcctcagc caagactatg acgacgcctt aaaaagaagc
    51361 cctcaggagc cagataggca ttaagtagga gatgaacgta gagatagaaa aaatcaatgc
    51421 acatgatctt atccaaaaaa caggggtaca aattaattga aaaagccttg agatgaaagt
    51481 tcctaaataa aaaagttcag aatgataggt atttctggct tatagtataa tatttacgcc
    51541 acagccttcc aatgattctt tttggaatca taatcaagga tggacattca caaaaatatt
    51601 ttgctcattc gatccgaata gattaacgac caatctagta agtaatatag attggtacgt
    51661 agtcaagctg cacataattt agatcaattt tagttctttt ttatatttct atgatctgct
    51721 catttacttt tatatgatgg cttacagtaa cataaataaa tatgataatc gctggtagtt
    51781 tatccaagag tactcaataa aatacaaaat ggattgttca agcgcctatc ataatgataa
    51841 aatgaaacaa ataaacgata caaggaggaa gagactttga gtaatggcag aacaattttt
    51901 tcattgtaaa aattgttcag ttgttaatct atgggatttc ggtagcatgg gcattttttt
    51961 atagaggaat cacaccaacc tggccgtttt atctcttgtt agtgggaata ttagtaggaa
    52021 attttgcccc tcttcactat cgtacgaact tttttccaac tgattctggc ctttttctta
    52081 aaacaaagac gcgaaaatta gaaaacatta ttgaaatgac aattacctgt ataatcattt
    52141 tcttagttat attcctaaac gtcttctaat aagaatctct taaaattagc ttgttacagt
    52201 tagttaccta gtttatataa cacagttatc ttatcttcct tatcaacatc ttttgccaac
    52261 tctttgtctt aaattcgaaa catataagca gagctacgaa caccaagact gtttcggctc
    52321 tgttttttgc atcaaattca ctcttatata gctgataatc tcggctcttt aaaagaaatt
    52381 attttcgctt cattttatta taaaaaggct aggaaccttg atgttatcag gttcctagcc
    52441 tttatttaag tattacccca cagacccttc catatagatt ttcaaattac acactagttt
    52501 aagataaaac aaacaaggaa aaacttacca tttactcaaa aaattaactc atataagtac
    52561 attagaatgt aaaatagact ggtcaaaatc tggtcaaaaa agtagatgtg acacaagttc
    52621 gcattcaaag cgatgatcca tgatttcgta atggtcaatt atatggatca aagccacgcg
    52681 acaagagcgc aatggatgtc tctaccgatt ggttgaataa acgaaataaa tagaatgtga
    52741 aaaaggatcg gacaaatgtt ccagtttgct cgatcctttt ttatttgaat tcattttcgc
    52801 tttatcaaac atcatttgat tactcagtta aatacttttg acatgagtac caatgaaaca
    52861 tttattcatt aaaattactt atttcttatc gcctttctag ccaatgccaa tccgccgatt
    52921 attccagcac tatcacctaa ttcgggcgta acaatgtagc tagcaattgg tggtatttca
    52981 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
    53041 ttcattacac caccgccaaa aataattcta tctggcgaaa gcatcagtgt tgtattgtat
    53101 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
    53161 tggcctttta cacccaatcg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
    53221 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
    53281 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
    53341 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
    53401 acatattcac catagcaagc cgcattaaca tctgttgtcc attcaatcgg aatattgaaa
    53461 tgttgcttta gcgttcctac aaaattgaaa ttctgccaag caagtttcgg tgttgatgtg
    53521 atatgaccgt atttctttga tttacgttga atatcaattg gaccaaaaca gccgaccccg
    53581 attcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
    53641 actggagtcg ttgtgggaaa actgactctt tccaaaatcg tcaattcctc agtactgact
    53701 gcacaaataa atttagtccc tccagcttca attgccccat atatcatcat aggcttctcc
    53761 cttcgaatat gtgtaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
    53821 gtttagtatt tcctgaagct tctgttgaat accaata
//
LOCUS       DAZKSU010000011        52450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000011 DAZKSU010000000
VERSION     DAZKSU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.011"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..795
                     /locus_tag="ACG8Z3_000670"
                     /pseudo
     CDS             1..795
                     /locus_tag="ACG8Z3_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
     gene            935..1861
                     /gene="cysK"
                     /locus_tag="ACG8Z3_000671"
     CDS             935..1861
                     /gene="cysK"
                     /locus_tag="ACG8Z3_000671"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0823014.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2097..2543
                     /locus_tag="ACG8Z3_000672"
     CDS             2097..2543
                     /locus_tag="ACG8Z3_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0823015.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2815..4167
                     /locus_tag="ACG8Z3_000673"
     CDS             2815..4167
                     /locus_tag="ACG8Z3_000673"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0823016.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            4338..4625
                     /locus_tag="ACG8Z3_000674"
     CDS             4338..4625
                     /locus_tag="ACG8Z3_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0823017.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4851..5294
                     /locus_tag="ACG8Z3_000675"
     CDS             4851..5294
                     /locus_tag="ACG8Z3_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0823018.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5300..6484)
                     /locus_tag="ACG8Z3_000676"
     CDS             complement(5300..6484)
                     /locus_tag="ACG8Z3_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0823019.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6752..7450
                     /locus_tag="ACG8Z3_000677"
     CDS             6752..7450
                     /locus_tag="ACG8Z3_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0823020.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7457..8134
                     /gene="nth"
                     /locus_tag="ACG8Z3_000678"
     CDS             7457..8134
                     /gene="nth"
                     /locus_tag="ACG8Z3_000678"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0823021.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8260..8793)
                     /locus_tag="ACG8Z3_000679"
     CDS             complement(8260..8793)
                     /locus_tag="ACG8Z3_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823022.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9090..11471)
                     /locus_tag="ACG8Z3_000680"
     CDS             complement(9090..11471)
                     /locus_tag="ACG8Z3_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0823023.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11461..12108)
                     /gene="recU"
                     /locus_tag="ACG8Z3_000681"
     CDS             complement(11461..12108)
                     /gene="recU"
                     /locus_tag="ACG8Z3_000681"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0823024.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12172..12711
                     /locus_tag="ACG8Z3_000682"
     CDS             12172..12711
                     /locus_tag="ACG8Z3_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0823025.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12801..13220
                     /gene="gpsB"
                     /locus_tag="ACG8Z3_000683"
     CDS             12801..13220
                     /gene="gpsB"
                     /locus_tag="ACG8Z3_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0823026.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13242..13609
                     /gene="rnpB"
                     /locus_tag="ACG8Z3_000684"
     ncRNA           13242..13609
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8Z3_000684"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13818..14984
                     /locus_tag="ACG8Z3_000685"
     CDS             13818..14984
                     /locus_tag="ACG8Z3_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0823027.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15030..16526
                     /locus_tag="ACG8Z3_000686"
     CDS             15030..16526
                     /locus_tag="ACG8Z3_000686"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0823028.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16607..17290
                     /locus_tag="ACG8Z3_000687"
     CDS             16607..17290
                     /locus_tag="ACG8Z3_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0823029.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17935..19125
                     /locus_tag="ACG8Z3_000688"
     CDS             17935..19125
                     /locus_tag="ACG8Z3_000688"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0823030.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19227..20180
                     /locus_tag="ACG8Z3_000689"
     CDS             19227..20180
                     /locus_tag="ACG8Z3_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0823031.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20249..21598
                     /locus_tag="ACG8Z3_000690"
     CDS             20249..21598
                     /locus_tag="ACG8Z3_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0823032.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21781..22104)
                     /locus_tag="ACG8Z3_000691"
     CDS             complement(21781..22104)
                     /locus_tag="ACG8Z3_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823033.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22233..23396
                     /locus_tag="ACG8Z3_000692"
     CDS             22233..23396
                     /locus_tag="ACG8Z3_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0823034.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23466..24815
                     /locus_tag="ACG8Z3_000693"
     CDS             23466..24815
                     /locus_tag="ACG8Z3_000693"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0823035.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24860..25291)
                     /locus_tag="ACG8Z3_000694"
     CDS             complement(24860..25291)
                     /locus_tag="ACG8Z3_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0823036.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25679..28321
                     /gene="alaS"
                     /locus_tag="ACG8Z3_000695"
     CDS             25679..28321
                     /gene="alaS"
                     /locus_tag="ACG8Z3_000695"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0823037.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28521..29117
                     /locus_tag="ACG8Z3_000696"
     CDS             28521..29117
                     /locus_tag="ACG8Z3_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0823038.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29239..30387
                     /locus_tag="ACG8Z3_000697"
     CDS             29239..30387
                     /locus_tag="ACG8Z3_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0823039.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30425..30838)
                     /locus_tag="ACG8Z3_000698"
     CDS             complement(30425..30838)
                     /locus_tag="ACG8Z3_000698"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0823040.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30917..31348
                     /locus_tag="ACG8Z3_000699"
     CDS             30917..31348
                     /locus_tag="ACG8Z3_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0823041.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31447..31680)
                     /locus_tag="ACG8Z3_000700"
     CDS             complement(31447..31680)
                     /locus_tag="ACG8Z3_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0823042.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31863..33533
                     /locus_tag="ACG8Z3_000701"
     CDS             31863..33533
                     /locus_tag="ACG8Z3_000701"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0823043.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33680..34066
                     /locus_tag="ACG8Z3_000702"
     CDS             33680..34066
                     /locus_tag="ACG8Z3_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823044.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34053..34751
                     /locus_tag="ACG8Z3_000703"
     CDS             34053..34751
                     /locus_tag="ACG8Z3_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0823045.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34756..35220
                     /gene="lspA"
                     /locus_tag="ACG8Z3_000704"
     CDS             34756..35220
                     /gene="lspA"
                     /locus_tag="ACG8Z3_000704"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0823046.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35217..36128
                     /locus_tag="ACG8Z3_000705"
     CDS             35217..36128
                     /locus_tag="ACG8Z3_000705"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0823047.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36363..36902
                     /gene="pyrR"
                     /locus_tag="ACG8Z3_000706"
     CDS             36363..36902
                     /gene="pyrR"
                     /locus_tag="ACG8Z3_000706"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0823048.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36916..38199
                     /locus_tag="ACG8Z3_000707"
     CDS             36916..38199
                     /locus_tag="ACG8Z3_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0823049.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38212..39138
                     /locus_tag="ACG8Z3_000708"
     CDS             38212..39138
                     /locus_tag="ACG8Z3_000708"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0823050.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39157..40437
                     /locus_tag="ACG8Z3_000709"
     CDS             39157..40437
                     /locus_tag="ACG8Z3_000709"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0823051.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40442..41518
                     /locus_tag="ACG8Z3_000710"
     CDS             40442..41518
                     /locus_tag="ACG8Z3_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0823052.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41523..44708
                     /gene="carB"
                     /locus_tag="ACG8Z3_000711"
     CDS             41523..44708
                     /gene="carB"
                     /locus_tag="ACG8Z3_000711"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0823053.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44762..45538
                     /locus_tag="ACG8Z3_000712"
     CDS             44762..45538
                     /locus_tag="ACG8Z3_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0823054.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45535..46467
                     /locus_tag="ACG8Z3_000713"
     CDS             45535..46467
                     /locus_tag="ACG8Z3_000713"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0823055.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46464..47180
                     /gene="pyrF"
                     /locus_tag="ACG8Z3_000714"
     CDS             46464..47180
                     /gene="pyrF"
                     /locus_tag="ACG8Z3_000714"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0823056.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47187..47816
                     /gene="pyrE"
                     /locus_tag="ACG8Z3_000715"
     CDS             47187..47816
                     /gene="pyrE"
                     /locus_tag="ACG8Z3_000715"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0823057.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47804..48118
                     /locus_tag="ACG8Z3_000716"
     CDS             47804..48118
                     /locus_tag="ACG8Z3_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823058.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48156..48797
                     /locus_tag="ACG8Z3_000717"
     CDS             48156..48797
                     /locus_tag="ACG8Z3_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0823059.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48840..49532
                     /locus_tag="ACG8Z3_000718"
     CDS             48840..49532
                     /locus_tag="ACG8Z3_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0823060.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49529..50431
                     /locus_tag="ACG8Z3_000719"
     CDS             49529..50431
                     /locus_tag="ACG8Z3_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0823061.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50589..52295)
                     /gene="efbA"
                     /locus_tag="ACG8Z3_000720"
     CDS             complement(50589..52295)
                     /gene="efbA"
                     /locus_tag="ACG8Z3_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0823062.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        17146 a         9140 c        11269 g        14895 t
ORIGIN      
        1 tggaacgagt cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc
       61 agatgaacaa ggatctcaaa cagttgctga tcctcaagcg tttatcgatc aaatcggctg
      121 gtctgcgtca gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc
      181 gttgttggaa agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt
      241 cggtgtgaaa ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaac
      301 ggacaatggg taacgatgaa tgaaccattc cgaaaatatt cttcttattg ggaatccttc
      361 caagaccatg cgaatgttct tcgttcaacc agtttttcca cgggcgatta tcattatgct
      421 ggcgtgtgga aaagcaatac aacaagtttt tatgatgcaa cagcgtactt gacaggtcgt
      481 tatgctaccg atccaaatta tggggccaaa ttgaactcat tgattaatac ttataattta
      541 acccgttttg atacgccatc aacaggttcg gctaccacaa gtacaacaac aacgactact
      601 acgacgactt caacgtcatc atcttcaaca acagatgtaa atacaaataa tggtcagaca
      661 tatacagtcg tgtcaggtga tacgctatgg gatattgcac aaaaatttgg catgtctgtt
      721 gatgatctaa tggcgaaaaa tggcatgtct atgtccagtt attctttatt agttggtcaa
      781 gaactaaacg tttgatttag ataaaaaggc aaagaaacta ccatatatgg tatatctttg
      841 tctttttttt gctagtcaaa gacgaatcac tcgccataaa tgaacagatt gtattatgat
      901 aagtaagaag agcaaagaca aggaggaatt gaagatgaca gctatctata actcagtcac
      961 cgaattgatt ggcaaaacgc caatcgtcaa attaaataaa atcgtaccag aagattcagc
     1021 agatgttttt gtaaagcttg agttttttaa tccaggagga agtgtaaaag atcgtattgc
     1081 tttaagtatg atagaaaaag cagaacacga tggtctactg aaaccaggtg atactatcat
     1141 tgaacctact tctggaaata caggaatcgg attgtcgatg gtaggagttg caaaaggata
     1201 taaagtaatc atcgtgatgc ctgagacgat gagtatcgaa agacgtttat tgatgaaagg
     1261 gtacggcgca gagttgattt tgactcctgg tgcggatggt atttctggat caatcagaga
     1321 agcagaacgt ttggcaaaag aaaacggcta ctttttacca ttacaatttg aaaatgaagc
     1381 aaatcctctt gtgcatgaga agacgactgg tcctgagata catcaagcat ttggagtaaa
     1441 tggcttggac gcgtttgttg caggtatcgg tacaggagga acgatcactg gcgctggccg
     1501 agaattaaaa cgtgtgtatc cgaaaatcga attaatcggg gtagaaccag cagaatctgc
     1561 tattttagaa gggaaagaag cgggacctca caaaatccaa ggaatcggta caggttttgt
     1621 tcctaaaaca ctggatactt ctgtgtatga taaagtcctt tctattagtg gagacgaagc
     1681 gatggaaaca gctagagaag ttgggcgaaa agaagggatt ttagtaggca tctcatctgg
     1741 cgctgcaatt gctgccgcgc taaaagtcgc aaaagaatta ggaaaaggca agaaggtatt
     1801 agctgtggtt cccgacaatg gtgaacgtta tttatctaca gccctttacc aagagatata
     1861 aggcacttgg tttttctcca gataggttta tgtaaaaaac ttttaaagat gaaaagaaca
     1921 cacaaaaatt tatgatgaga aaaattattt gaatagagac gtaaaatagt gaacgcgact
     1981 gttttacgtc ttttttcatt aaaatacaat aaaattgaat tttcttttca tgttaagtgg
     2041 attttaaaat gtaaaaatag tatgataaga cgagaataac aacgtggggt gtttacatgg
     2101 aaaataagtt agtcgaagag gttatcgaaa agttaaaaaa agagaacatt cgaatcacac
     2161 ctcaacggta cgcggtttta gaatatttga ttgaacatca ttcccatcca acggctgatg
     2221 agatttataa agcacttgaa catcggtttc caaatatgag tgttgcgact gtttacaata
     2281 accttcgctt atttacttcg attggatttg ttcaagaaat gaagtacggc gatgcttcta
     2341 gccgatttga tttcagttca aagcgtcatt atcatgtaat ttgtgaaaac tgcggtaaga
     2401 ttgttgattt tcattaccca gggcttgaag atatagaaat ggctgcgagc agactgactg
     2461 attttgagat taatgaacat cggttggaat tgtatggact ctgtccggat tgtaaagagt
     2521 cagcaaaaaa ggcgcaaatg taaaaacagt ttctataagt gttataccta aaaaaagcta
     2581 gctgtgtaaa aaaacgctag cttttttatt tttttgtctt ttattcacaa tcttagatat
     2641 caaagtgaat attatcacaa taagaatctt gtagatattg ggttcttatg cttgaaaagg
     2701 aatgaaacta caaacaaacc tttgtgaatt cacaagcatt aaactgttgt atccgtttta
     2761 tttctgttat agtagttttg tcgaaggaca aaatgttaat cggagggacc ataaatgaaa
     2821 gtagtagtaa ttggctgtac tcatgcaggt actgcagctg taaaaagtat tttaaaaaat
     2881 catccagaag cagaagtgac agtatatgaa agaaatgaca atatttcttt cttatcttgc
     2941 ggtatcgcac tttatgtcgg cggagtagta aaggacccag caggcttgtt ctactcaaat
     3001 cctgaagaat tatcttcatt aggagcaaca gttaaaatgg aacacgatgt aacagacgtc
     3061 gatacagaaa ataaaacagt aacggcaaaa gatctgaaaa caggagaaac agaaacggtt
     3121 tcttatgata aattagtgat gacaactggt tcatggccta ttatcccacc gattagtgga
     3181 atcgatgcga aaaatgtact cctatgcaaa aactacaatc aagcaaatga aattattgca
     3241 caagcgaaag aagcaaaacg tgtagtaatt gttggtggtg gatatatcgg tattgaatta
     3301 gtagaagctt ttgtcgaatc tggaaaacaa gtgacattga ttgatggatt agatcgcatc
     3361 ttgaataaat atctagataa accattcaca gatatcttag aaaaagaatt gacagaccgc
     3421 ggtgttactc ttgcgctagg cgaaaatgtt actgaattta tcacagatga agaaggaaaa
     3481 gtgaaacaag tagctacacc aacagataca tttgatacag atatggtcat tttatgtgtg
     3541 ggcttccgtc caaatacaaa actgatcgaa ggaaaagtag atacactgcc aaacggggca
     3601 atcaaagtga atgaatacat gcagacaagt gatccagaca tttttgcagc tggagactct
     3661 gcagtagtca actacaatcc aagtggcacg caaaactata ttcctttggc aacaaatgcc
     3721 gttcgtcaag gtttacttgt gggaaataac ttgacagatc ataaaatggc gtatcgtggt
     3781 actcaaggaa cttcaggact ttacctattt ggctggacga tcggttcgac aggtgtgacc
     3841 aaagaaagcg caactttgaa cgatctagaa gtccaagcga ctgtatttga agataattat
     3901 cgtccagagt tcatgccaac tactgaaaaa gtaatgatgg agctagtcta tgaaaaagga
     3961 acgaatcgta tcgtaggtgc tcagttcatg tccaaatacg acattacaca atcagcaaat
     4021 accatgtctt tagctgttca aaataaaatg actgtagaag atctcgcact ttcagatttc
     4081 ttcttccaac cacattttga ccgcccatgg aattacttga acctacttgc acaagaagcg
     4141 ctaggcgaaa tcgaaactgc acaataaaga aaaagggttg tgacacaatt ttgtcacgac
     4201 cctctgtctt tttttgtgaa aagtacaatt agttgtcttt cttcgaattt tttgtcacaa
     4261 taagcagcaa agataaagct gttacataaa agaacaagca gctttatttg attttgaaaa
     4321 gaaaggtgag aaagaagatg aataaagaat attttgtcca acaagatgca tttttagaag
     4381 attataaaag tgcttcgaag aaattaacga tcaatattga tgagttggca gatcaagtac
     4441 tctctcagtt gaatgaaacg caagaggaat attacacgat gccagcaaat gacgccaaag
     4501 ataatgtttc tcatcgtttt cctttcagta gacgcatcta ctcagatgaa aatgacggca
     4561 cattgaatac agagtacaca tatgaaggtc agccatatgt gctagaaact gatgatgatc
     4621 tttaaagaca gtttttgaaa aggccgctca tccttaagtg atgacaaaaa atcatcacaa
     4681 ttaccgatga ctgtctcttt actatctttt aatcagaaaa aggggttatg acaagctttg
     4741 tcaacccctt tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt
     4801 tgaataggaa acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa
     4861 gacaactgct aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac
     4921 cagaaatgga aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata
     4981 tcgggaaaat ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag
     5041 gagccatcag taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa
     5101 gcgaagagaa taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga
     5161 atagacacga gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata
     5221 tgtcaaaaga agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac
     5281 gaagtagcaa atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca
     5341 aaaatccgaa taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg
     5401 ggccgatgac gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct
     5461 ggttatctga gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac
     5521 cgaaaccata acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata
     5581 atgtgatgaa tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt
     5641 ctgaaaattt tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc
     5701 caatcaaaga gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa
     5761 attgagaaaa gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca
     5821 ataaaggaat aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat
     5881 gggagaaact taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt
     5941 agccataaac ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc
     6001 ccaactcagc taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag
     6061 cacccatcca tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag
     6121 catataaggt ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca
     6181 tccatatcga atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga
     6241 taataaggat cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata
     6301 agaaggcagc tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta
     6361 tggagtgaga ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta
     6421 atccagtgaa taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt
     6481 acatgtaaaa actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa
     6541 ttttgtttcc ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga
     6601 aaagtgaaga tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa
     6661 tctgaccgat cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt
     6721 acgtatactg tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc
     6781 tggacaaaca gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc
     6841 tgaagaattt ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc
     6901 tgatttgcag atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact
     6961 ggatcgtctc gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa
     7021 agcagatcga tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca
     7081 agaaaaaaaa tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt
     7141 tgaacaagaa tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct
     7201 agaagaagac cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa
     7261 tcaggcttat agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct
     7321 acgtacaaaa caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag
     7381 agaagtagaa cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt
     7441 ggaggagtag cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta
     7501 tggaatgttt cccgaagcac atggagaact caagcacaat aatccatttg agttattgat
     7561 tgcagtgatc ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt
     7621 gtttgcctct tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct
     7681 taaaataaaa acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca
     7741 gcagctgatt gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct
     7801 tcctggtgta ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc
     7861 aatcgcagta gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga
     7921 tgctacggtc atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt
     7981 aaagacacat catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa
     8041 gtgcgaggta tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa
     8101 agaaaaggcg ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa
     8161 tgtatgaatt atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa
     8221 aagtaaatgt acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa
     8281 ataaaagttg ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat
     8341 acctataaaa taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa
     8401 aggtgtaata aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact
     8461 aatggatttg ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca
     8521 taaaaaagtg agaatcattg catgagagct tctttccata aattttcctt taagagtagt
     8581 gcttaaaagt acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt
     8641 taataataaa caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga
     8701 taaaaaaaga tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat
     8761 gaagccttct ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac
     8821 aatgggatta ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat
     8881 tataaacaag taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt
     8941 tttaagctaa atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta
     9001 atacagcttg gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa
     9061 ccacgcttgt agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg
     9121 gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca
     9181 ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt
     9241 gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat
     9301 tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga
     9361 gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct
     9421 gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc
     9481 cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag
     9541 gctacgtgtg acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg
     9601 gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa
     9661 ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga
     9721 tataacccag cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg
     9781 tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt
     9841 tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct
     9901 ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc
     9961 ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg
    10021 atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg
    10081 actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat
    10141 tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta
    10201 atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt
    10261 ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg
    10321 tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata
    10381 agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg
    10441 atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct
    10501 gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta
    10561 tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt
    10621 acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac
    10681 aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa
    10741 ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc
    10801 ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact
    10861 ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc
    10921 atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag
    10981 gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag
    11041 ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg
    11101 cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt
    11161 ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg
    11221 gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac
    11281 cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg
    11341 atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa
    11401 ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt
    11461 tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc
    11521 gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat
    11581 caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa
    11641 atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca
    11701 gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt
    11761 aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg
    11821 atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc
    11881 agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat
    11941 aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg
    12001 catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg
    12061 agaggcattc ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac
    12121 tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag
    12181 tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat
    12241 gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa
    12301 gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa
    12361 atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt
    12421 tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa
    12481 ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag
    12541 aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa
    12601 ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc
    12661 tacgaaattc ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg
    12721 tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa
    12781 tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac
    12841 aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca
    12901 atatcatcaa agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg
    12961 atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg
    13021 tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct
    13081 tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca
    13141 aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg
    13201 aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt
    13261 ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc
    13321 gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa
    13381 ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac
    13441 ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct
    13501 ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc
    13561 tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca
    13621 tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa
    13681 gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg
    13741 gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat
    13801 aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa
    13861 gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg
    13921 agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt
    13981 tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg
    14041 aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt
    14101 ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga
    14161 tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac
    14221 cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga
    14281 tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag
    14341 gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa
    14401 aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct
    14461 taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt
    14521 ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg
    14581 atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag
    14641 ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag
    14701 acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc
    14761 taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat
    14821 taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac
    14881 aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt
    14941 attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc
    15001 attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa
    15061 aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta
    15121 tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt
    15181 tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg
    15241 aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg
    15301 aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg
    15361 gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc
    15421 aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt
    15481 atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat
    15541 ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg
    15601 aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag
    15661 tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc
    15721 acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa
    15781 cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac
    15841 aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc
    15901 atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa
    15961 agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca
    16021 aattttatcg tttattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt
    16081 tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag
    16141 gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa
    16201 tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct
    16261 ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt
    16321 tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa
    16381 actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca
    16441 tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt
    16501 attttaatgt atatcaggta aaataaaata caatttacaa agatagctga aattcatcta
    16561 atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca
    16621 agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga
    16681 tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga
    16741 ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa
    16801 aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga
    16861 ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc
    16921 tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc
    16981 attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat
    17041 ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac
    17101 gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga
    17161 ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt
    17221 ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa
    17281 caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc
    17341 cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc
    17401 gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt
    17461 gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt
    17521 tgtcacagtc cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa
    17581 aattaaaaat tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa
    17641 taaataaatt aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt
    17701 gtgttgtatt tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc
    17761 atactatata agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa
    17821 ttctcataat cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga
    17881 cacgatatat gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct
    17941 tccaaagaac taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta
    18001 tacaatacga tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa
    18061 aagatgatca atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa
    18121 aaagcatgta ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc
    18181 aatggttcgc tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca
    18241 gaagcagcaa tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta
    18301 atgacaaaag aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt
    18361 tgccgcttat ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag
    18421 aaaaaagcaa aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat
    18481 ggtgttttct ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa
    18541 aaatatggac tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt
    18601 gcaggaaccg tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta
    18661 tcgaaagcta taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg
    18721 ttgaaagcta gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca
    18781 gctgcattgg cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg
    18841 gaaaatgcta attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct
    18901 gaaacaccta ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag
    18961 aagctgatcg aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg
    19021 acaggcagga tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca
    19081 gtagcagttt atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt
    19141 tcgtctattt aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt
    19201 agtaaaaaag atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac
    19261 aaattggttc agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg
    19321 cgacagatat acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg
    19381 taatggatta cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc
    19441 atttggctgc tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga
    19501 acgtgactgg gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta
    19561 cgccaagttc aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata
    19621 ctcttcagcg tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg
    19681 attattatta tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt
    19741 cttatgcaac cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag
    19801 caatcaaaga aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt
    19861 atatgccaga tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg
    19921 ttcatcgaaa tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat
    19981 ctattcaaaa aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt
    20041 caatagcaga atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct
    20101 tttcgccgaa atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa
    20161 aattggtaga gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga
    20221 ttggctatgg tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga
    20281 agtaaaaata gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt
    20341 tatgggcaca gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac
    20401 aggatccgcc agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc
    20461 aggactaaca ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata
    20521 tatcgaacgg atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat
    20581 ttattttcca gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct
    20641 atttggtctt tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct
    20701 gttgatcaat tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg
    20761 caagcttatt cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga
    20821 cttccaactg tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc
    20881 gggcttgcta gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg
    20941 agaattgaaa aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat
    21001 catgatcgtt tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt
    21061 cgcaggaaac agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg
    21121 aagactggtc acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct
    21181 tactggaatg cgtctccctt atgttatggc aaaagaaaac aacctgcctt ttagcaagct
    21241 ctttgctaaa cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt
    21301 catcgctatt ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt
    21361 tgtcatttgg attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga
    21421 acctgatttg tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat
    21481 cattggaggg acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat
    21541 ggggatagta ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct
    21601 aaaacattcg aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat
    21661 gaggagtacg tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt
    21721 ttgtctacga tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt
    21781 ttactttgat ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac
    21841 caagcaacca gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt
    21901 agacaaagca tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag
    21961 attgaagact agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt
    22021 agttcttttt cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc
    22081 aaaaacaaag tacatattca gcatctcgac accccctttg tgtttatctt actttacttt
    22141 gaaagaaatg agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat
    22201 aagagagata caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt
    22261 ttgcgctgat tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg
    22321 aatatcgaaa gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt
    22381 tttatttatt gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg
    22441 cccaaatgac agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag
    22501 acaacacggt attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg
    22561 tatttttgga accagctttt aatgttttat tactcatacc atttggcatt tatttaagat
    22621 attatttccg gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt
    22681 ttgaattgac gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg
    22741 atgtgaatga tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt
    22801 tatttacatt tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa
    22861 agactgtctc attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc
    22921 ttattaattt ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt
    22981 ctcatatctg gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa
    23041 ccaatggatg gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg
    23101 aacgaatttc tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg
    23161 gtttaagctg gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt
    23221 tgcagatgag tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt
    23281 taaatatctt gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc
    23341 acacatatac tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga
    23401 gcaaaaacgc tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg
    23461 cacgaatgaa taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg
    23521 caaagggctt caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag
    23581 gaaaaaatat cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc
    23641 ctttgtttga aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa
    23701 gtagggaact agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac
    23761 cggagatccg tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc
    23821 tgcatcacca acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga
    23881 atgaacaagc gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga
    23941 cattggatat gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc
    24001 tgcagttttt agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat
    24061 acatggaaaa tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg
    24121 aaaattggct gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga
    24181 caattggtca tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa
    24241 ttgccgatta cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc
    24301 caagagagag aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag
    24361 cgactgattt agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag
    24421 aaattccaga agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct
    24481 taaaaggaac ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag
    24541 aaaaattggg cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat
    24601 acgatcgtaa tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga
    24661 ttggtcttgt gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt
    24721 gggcaattgc agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta
    24781 ctgctcgtaa aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc
    24841 gtgtcacaat ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat
    24901 cttcaataaa aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg
    24961 tcatttcagc tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag
    25021 cttcaagcaa ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt
    25081 ccggcttgaa gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt
    25141 gatcaagaaa tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg
    25201 caggaatccc ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta
    25261 tccattcatc cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag
    25321 atgtattgat tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat
    25381 gaaaataaaa attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga
    25441 aaaggatatg atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat
    25501 agtcactaat caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg
    25561 ttatcgagtt taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt
    25621 tcgtccttgg ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat
    25681 gaaacaatta agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg
    25741 acatacgatt gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat
    25801 caactctggt gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc
    25861 aagaatcaca aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac
    25921 agcacggcac catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa
    25981 aaatgaagcc atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga
    26041 tccagaaaaa ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca
    26101 tgaagaagtt ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat
    26161 cggtgcaggc ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa
    26221 cgatttagca gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga
    26281 aatctggaac ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact
    26341 tcctcataaa aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga
    26401 tgcgtcaacg aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct
    26461 aagcaaacaa gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc
    26521 agatcatatt cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg
    26581 gagagggtat gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg
    26641 tattgaacaa gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta
    26701 ttatcctgaa gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga
    26761 acgtttcaat gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt
    26821 aaaaaacgaa aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata
    26881 tggcttcccg gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca
    26941 tgctggtttt gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac
    27001 tgaaaaatca atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt
    27061 cattggatac acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc
    27121 tcttttgaat gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta
    27181 tgcagaaatg ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat
    27241 cgtagcagtt gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga
    27301 agtaaaagca ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg
    27361 caatcggatc atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt
    27421 acttggcgag catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga
    27481 ctttacccac tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa
    27541 cgaaaaaatc tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa
    27601 aaacatgggt gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa
    27661 tattggcggc tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat
    27721 cggaatcttc aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc
    27781 tgtaacaagt aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt
    27841 agcaacaatt gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca
    27901 acagcaagta agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca
    27961 acaagctggt gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc
    28021 acaagtaaaa gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa
    28081 aaatatttca gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc
    28141 ggcaatgacg aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat
    28201 cgcaccgaaa gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa
    28261 aaatcctgct ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata
    28321 gggcgtttac gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg
    28381 ggcgaagaca aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa
    28441 atggtcggat tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat
    28501 aaataaaaag gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg
    28561 cgatcttgtt agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat
    28621 taggattcat tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag
    28681 gcccaaaaga tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct
    28741 atgccatgcc aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag
    28801 ttcccagagt acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa
    28861 ataaatcaat aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag
    28921 tcttagtgtt aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg
    28981 tagactctgc tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag
    29041 aagcaatagg tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac
    29101 cgttgaaacc agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa
    29161 aggaagagag gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa
    29221 ctagaagggg taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa
    29281 cagcggacaa aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg
    29341 tttgtctcta agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca
    29401 gtgaacagtc tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct
    29461 gcaaagccag cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg
    29521 ttatttgttt cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa
    29581 aaaatcatcc atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt
    29641 tccatctttt tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat
    29701 tctgaaacat tgagagctac agcaacagat agttataatg atgtctttac tacactggct
    29761 gtattgcttt cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt
    29821 tttgctttag cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac
    29881 gaactattag gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat
    29941 gcttgcgatg atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa
    30001 cgctttgcat cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag
    30061 attattgatc tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac
    30121 ttggatccaa tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa
    30181 attcttcttg aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga
    30241 aaaacgctgc aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta
    30301 gaaagaatct tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg
    30361 tttgatcaca attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt
    30421 taatttaaag agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat
    30481 ctgctccttt atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata
    30541 gaagttcttg gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt
    30601 cgaccgtaga aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt
    30661 cgtttttctt tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg
    30721 tccctataga aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc
    30781 cacctccact aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa
    30841 aaagagattc tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc
    30901 ggtgagaggt tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat
    30961 catctattgg tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa
    31021 cacaggtcct tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct
    31081 aggtttgcgg cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt
    31141 ttggaaagac cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg
    31201 ggtgcaaatg atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga
    31261 tcaagtagtc ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta
    31321 tccttatacg aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt
    31381 ttacaggaaa agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt
    31441 gtttatctat tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc
    31501 agcttgtgtt cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa
    31561 agttttgaat ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc
    31621 ataactgata aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa
    31681 agcactcacg acctttctaa agtctgatca tagccttcat tataaagagt atttataagt
    31741 acgtcaaata aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata
    31801 attggcatat cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc
    31861 aaatgaagaa tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg
    31921 ctagtaaatt agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa
    31981 tcaatttttc tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta
    32041 cttccatcaa tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag
    32101 acgcgctcaa taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc
    32161 ctgtgatggg aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg
    32221 aagatatcaa cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat
    32281 tatctgctct tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc
    32341 gagtgatttg gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg
    32401 gcttgggtgg gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca
    32461 gcgaaatcat ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg
    32521 caaggatcgt tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag
    32581 cagaaggcgc tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa
    32641 tttacggaac accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca
    32701 atagtatttt agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag
    32761 gatttggtgc agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa
    32821 aagcgccgga tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa
    32881 tgaaaaaaga tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt
    32941 taaagcgcca tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg
    33001 aattcgtaac agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg
    33061 ggattcttgc caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag
    33121 caaaagctgt agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc
    33181 tagaagaaac gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg
    33241 gtgttgtttt cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg
    33301 acaaactgcc gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc
    33361 taggtgcacc ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag
    33421 gatttttagt agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg
    33481 ctgcattgaa tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat
    33541 tataatctac catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg
    33601 aattaatttt gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa
    33661 agcataatag gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat
    33721 gggtagctcg attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca
    33781 aaagataacg cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa
    33841 aagccccgtt tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt
    33901 gattcttcaa atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca
    33961 aaatttattt ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa
    34021 aaaccgggca tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca
    34081 gtttcaatcg aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat
    34141 ttaccgaact tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag
    34201 atgatctttt acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg
    34261 attttgtcat tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag
    34321 aactgattca gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac
    34381 aagatcttcc gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc
    34441 cgttatacaa taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc
    34501 tagcaaaaga aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga
    34561 tcatccaaaa cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg
    34621 aggttgaagc gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg
    34681 ggaatcccga tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag
    34741 aaaaggattg attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg
    34801 gatcagttgg tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt
    34861 ataccaggta ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta
    34921 gaagggaaga tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca
    34981 ttgatgatta aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt
    35041 ctagctggtg cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg
    35101 ttccagacag attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt
    35161 ggagtgattt gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga
    35221 cagaacttaa aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt
    35281 tattcaccga ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg
    35341 tcaatggaca gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct
    35401 tgatccccga accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt
    35461 acgaagatga agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag
    35521 caggtcatac gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta
    35581 cgatcaacgg cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac
    35641 tgctaatggt tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata
    35701 agacctcttt gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa
    35761 ccatcaatgc gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag
    35821 atgggaagtc ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg
    35881 ttgaattaaa actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg
    35941 gttatccgtt agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc
    36001 agtttctcca tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct
    36061 ttgaagcacc tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg
    36121 cattttaatt tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt
    36181 cctttaagtt tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc
    36241 ataagtagat tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta
    36301 aacattcctc ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga
    36361 aaatgcaagc aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta
    36421 cttatgagat tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa
    36481 cacgaggaat ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg
    36541 atattcctgt aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag
    36601 aagaaccgga acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt
    36661 tgattgatga tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg
    36721 attttggcag accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat
    36781 tgccaatccg tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt
    36841 tagtagagat gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt
    36901 agaggagaga gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat
    36961 atccacgatc gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg
    37021 atgttcggag caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta
    37081 gtcagttcag gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg
    37141 tacttaggaa gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt
    37201 tatccagcga ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg
    37261 atcatcaaaa agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt
    37321 ccagtcatca tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat
    37381 aatcagggca cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca
    37441 attttcttca acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt
    37501 attagcggat acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac
    37561 gctgcctggt ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta
    37621 tatcttggag caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc
    37681 ggccatctta tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt
    37741 cataagacgt tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct
    37801 cctgtaacaa gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg
    37861 tttgtgatcg gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc
    37921 gctttgattt taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga
    37981 gtaattgccg caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag
    38041 aaaaatctgt tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa
    38101 gtaggcacat ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat
    38161 cttatcttac cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc
    38221 aaatcagaac gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa
    38281 gttatgggct tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa
    38341 aagcgccagt actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc
    38401 ttcgatgtag cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc
    38461 tctgtccaaa aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta
    38521 gacgtagcgg ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa
    38581 acaatccagt gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc
    38641 ttgcttgatt tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc
    38701 atcgtaggtg atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt
    38761 ctaggcgcac aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg
    38821 tacggacatt atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt
    38881 cgggtgcaac acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag
    38941 gaacatgggt taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac
    39001 ccagcaccag ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg
    39061 cggatcgttc agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg
    39121 ctaaatggaa aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt
    39181 cagatcaata cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga
    39241 atcaaagcaa tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag
    39301 ggacaattga tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt
    39361 acatacaagg agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact
    39421 gtatgcgcga tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta
    39481 tatgacatca ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa
    39541 aatctgcgaa gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct
    39601 ttcacaaatg acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa
    39661 gctgcaaaaa acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc
    39721 ggcgttatgc acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta
    39781 accgagtctt ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac
    39841 catgtttgcc atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca
    39901 ggaatccacg taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt
    39961 cccgaagatt tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa
    40021 gctttgatcg aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc
    40081 catggattag aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc
    40141 gaaacagcgt ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa
    40201 caagtgatca attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg
    40261 ctaacggttg gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc
    40321 gataaagaag actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa
    40381 ggcgaaacac aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag
    40441 tatggaacgt ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc
    40501 agatgctagt gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac
    40561 tatcactgat caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa
    40621 ttatggggta aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt
    40681 aaaagaacat gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt
    40741 gaaacaaaaa ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat
    40801 ccgtgaagtc ggaacgatga aagctagtat agttgatgct ggagattcct tcgaacatgc
    40861 atttgatcag ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa
    40921 caaaccttat ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa
    40981 acatagtatt ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa
    41041 cacagatgcc gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc
    41101 tggtgatcca aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt
    41161 accgattttt ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac
    41221 ctataaaatg aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg
    41281 aagaattgat tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc
    41341 agaaaaactc atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca
    41401 cagagattat cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga
    41461 tgcactacat ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac
    41521 agatgccgaa acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca
    41581 ttggtcaagc cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag
    41641 aaggatatga agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag
    41701 tggcagatca agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa
    41761 aagagcgtcc agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa
    41821 tggagctggc agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc
    41881 tttctgcgat cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga
    41941 atcagccaat ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa
    42001 ataaaatcgg ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag
    42061 gtatgtgtga aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc
    42121 ccgctaccca atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag
    42181 tgatgagaga ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg
    42241 tgggtatcca tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg
    42301 aatatcaaat gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag
    42361 gctgtaatgt ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca
    42421 atccgcgtgt ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta
    42481 agctagctgc taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag
    42541 gaacgacttt tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct
    42601 ggccgtttga taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg
    42661 gtgaagtgat ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt
    42721 tagaaatcgg tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc
    42781 taatccaaaa agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta
    42841 gaagagggta caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag
    42901 ataaactgtt gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca
    42961 agatattgaa aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg
    43021 aaataaacga agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata
    43081 agatggttga cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct
    43141 atgaatatga aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg
    43201 gtcctatccg tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag
    43261 caatccagca ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga
    43321 ctgacttttc tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga
    43381 atgtgatcga gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa
    43441 tcaacttagc cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag
    43501 acttggatcg tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc
    43561 ctcaaccttt aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa
    43621 ttggttatcc agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg
    43681 ttgaaaacca gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac
    43741 atccagtact agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg
    43801 acggtgaaac agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt
    43861 caggggattc aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga
    43921 tcgaagatta cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc
    43981 agtttgtcat ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga
    44041 ctgttccatt cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta
    44101 ttttgggaga gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag
    44161 aagttcatgt taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact
    44221 tagggcctga aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag
    44281 ctttgtacaa ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat
    44341 ttacgattgc ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa
    44401 ttggctatag tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg
    44461 tcgttgaaac agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa
    44521 taagagatgg taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt
    44581 ctaaagacgg tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt
    44641 ctttagacac agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc
    44701 ctatttaacg gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca
    44761 gatgaaacaa gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga
    44821 aatgacattg aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat
    44881 tctcgtgccg cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca
    44941 cgaaacaaat acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc
    45001 tactctttct gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt
    45061 gtccaatctt catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc
    45121 tctatatgag ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg
    45181 ttttgccaca catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag
    45241 aatcgctact gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg
    45301 aacgaagacg gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt
    45361 ggaacagctg ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg
    45421 tgggataggg gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa
    45481 aagtgtgaaa gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat
    45541 gagaatcctt tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca
    45601 agcggctgtt tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga
    45661 tcgatcatgg tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg
    45721 gcggaaacac catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt
    45781 gttatgcata aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat
    45841 gttgctggtg cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca
    45901 aatgtgaaag caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct
    45961 tttggaacag attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct
    46021 gttcctgtct atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca
    46081 atcgaagctg gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt
    46141 gaccttaaga caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca
    46201 atcaagcctg tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc
    46261 attgggatgg gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca
    46321 agtgctgtag ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt
    46381 aaagagctcc ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa
    46441 gaagtgagag aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag
    46501 atttttcaac taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat
    46561 tgtttgtgaa aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc
    46621 taaaagaggc tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg
    46681 tcgaaaaagc aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg
    46741 ctggcggtat tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag
    46801 gtggaaaacg tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa
    46861 tgcatgccga tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct
    46921 gtgccaaaaa agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca
    46981 aagaaatggc aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag
    47041 tcgcaggaga tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt
    47101 ttatcgttat aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga
    47161 ttcaaacaga atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat
    47221 tagaaattga agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca
    47281 aaagcccaat ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga
    47341 tcgcaaaagg attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg
    47401 gaactgcaac agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa
    47461 tggtctatat tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta
    47521 tatttgaagg tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct
    47581 tggaagcagc tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct
    47641 ttacgtatga gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga
    47701 cgttgaccaa ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc
    47761 aagaattaac tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc
    47821 gcttgatgaa gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa
    47881 aatccaaaaa ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg
    47941 tgatcagttt ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag
    48001 aataatctct tctctttttt ctattatata tagattttct ctattttttg gtaagggagc
    48061 tgtttcaaaa atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata
    48121 ttcggaaaaa gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag
    48181 gattgaacaa tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata
    48241 cagttgcagc acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag
    48301 aagcgaatgg acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact
    48361 acacagagcg ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa
    48421 gagggatgct gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc
    48481 cagctgagtt ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag
    48541 aaggagaact attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa
    48601 tcatcaaaaa taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga
    48661 ttaaaagctt tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac
    48721 tattgaacca ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa
    48781 agaaagaaga aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata
    48841 tggatgtcga atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg
    48901 attggtttgc gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg
    48961 aagaaaccaa aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg
    49021 acaaggaatt caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt
    49081 tcaagaaaga acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca
    49141 tcaacggaaa acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga
    49201 atctagttgt gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag
    49261 aaaatgggga aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct
    49321 ttccggagca aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg
    49381 ggccaatcca atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc
    49441 ccttcgatgg tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag
    49501 aaatttatta tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc
    49561 tgtagcaaac aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag
    49621 tctttcagct ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac
    49681 gaatcgaggg gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat
    49741 attggtccag cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt
    49801 ttcgatcgct gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga
    49861 attcaaagac gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga
    49921 agaagtcaaa gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc
    49981 tggtatcaga agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa
    50041 aacgcatatt tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga
    50101 agaattgtta tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt
    50161 ttatgaagac cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg
    50221 tactttgatg aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat
    50281 tggagggatt cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt
    50341 ttatatcatt tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga
    50401 gcttactcgg ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga
    50461 acaccgttga tcaaaaaaga ggggttgtga cagaattttg tcacaactcc tctttttgta
    50521 gcaaacaatt tataactaat tgctgttgtg ggaaagtacg caaatatgat tctagaaaca
    50581 ctcattgatt acctgttttg tttcatagct gtaatttttt cttcttctgg tgtcacgata
    50641 atcgtctttt ggttttcata aataacataa ccaggcttgg cgccatttgg ttttcggata
    50701 tgctttacct gtacaagatc aaccggcact tgggcagagt atcggtattt tgaaaaatag
    50761 gcagctaact cggcagcttc tgtaattgtt tcatctgagg gcttgtcact tttaatgatg
    50821 acgtgcgaac cgggtatatt ttttgcatga agccaatagt cggttttttt agctgtcttc
    50881 attgtcagct ggtcattttg aagattgttt ttaccaacaa gaatgagcgt tccgtcagaa
    50941 gaaaagtatt gatcaggctg agatggtttc ttacgttttt gtttttttaa gttcttcttt
    51001 ttcaggtatc cttctgaagt caattcttct ttaattgctt cgatatccat tgggccggca
    51061 atttccagtt gagataagac agattcaaga tattggattt catctttggc ttcttggatt
    51121 tgttcgccaa tcagcttaac agcgttcttc agcttttggt atcgatggaa atatttctgt
    51181 gcattttgat ttggtgtcaa tgccggatct agtgctattt tgattggccg atcttcttca
    51241 taataattag gtaaaacaac ttcttttgcg ccgcgtggta cttgtgtcag aaaagtagta
    51301 agtagttcgc catttctacg gtaattctca gcgttttcgg attctttgag tgtttgttca
    51361 cgttttttca atttattttt atttcgtttt agttcatttt cgatttttcg aatcagttct
    51421 ccgccttgtt gttttgcccg atctttctct gctttttcgt gataatagct atctaataat
    51481 tgactgagtg ttggataagc tgttacagca gatgcctgtt cggacaatac ctgataagca
    51541 atcggtgtga aaaaatcttt attttccgtt tcgtaaaaag taggaatagg ctgatggctg
    51601 atggctgaga aaaattcgtc ccatacaacc atcttttcat taggacgttc cagtaatctt
    51661 ttggtcagct cttgagcgat atcgaaaccg attccctgaa actgacgttg gattgctttt
    51721 ggatctagtt cagtttggga aagccgctga aagatttttt ctttctcgga gctaaatggg
    51781 ttcaagacct cttgttttgg cggagcaaca tattctacac caggaagcaa agaacgataa
    51841 gtgttttgag agcttccaat gtgtttgatc gcatccagta tttttccagt ttctcgatta
    51901 actaagacga tcgtactatg acgtcccatt aattcaacga tcaatatgat attttgtaga
    51961 tcacccaact cgtttcgttt agcaaagtgg aaatggatca ctcgatcatt ttcgatttgt
    52021 tcgatgtctt ctaaaatagc gccatccaaa tattttcgaa gcatcatcac gaagttaggc
    52081 ggattatcag gattctgata atcgatttgg gtaatctgta cacgagcata acttgggtgc
    52141 gcagataata ataatcgatg gtttttgccg cgtgaacgaa tcaccagcac gatttcattt
    52201 tcataaggct gatggatttt cgagatccgg ccagatagta acgtttcacg caattcgttg
    52261 accatcgcgt gggtaaatac accatcaaag gacatggttc attcctcatt tcttttcata
    52321 agtatattca ttataacgaa aaaaagaaga aaaaccaatt tggccgaata gtaaagcaaa
    52381 agtctagaaa aatcttggac taattacata aaaaacagtg tttctatcgt tgaaatccaa
    52441 atttttaggt
//
LOCUS       DAZKSU010000012        50116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000012 DAZKSU010000000
VERSION     DAZKSU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.012"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..554)
                     /locus_tag="ACG8Z3_000721"
     CDS             complement(<1..554)
                     /locus_tag="ACG8Z3_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0823063.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIE"
     gene            complement(708..1238)
                     /locus_tag="ACG8Z3_000722"
     CDS             complement(708..1238)
                     /locus_tag="ACG8Z3_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0823064.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(1337..1687)
                     /locus_tag="ACG8Z3_000723"
     CDS             complement(1337..1687)
                     /locus_tag="ACG8Z3_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0823065.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1844..3373)
                     /gene="ahpF"
                     /locus_tag="ACG8Z3_000724"
     CDS             complement(1844..3373)
                     /gene="ahpF"
                     /locus_tag="ACG8Z3_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0823066.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(3383..3946)
                     /gene="ahpC"
                     /locus_tag="ACG8Z3_000725"
     CDS             complement(3383..3946)
                     /gene="ahpC"
                     /locus_tag="ACG8Z3_000725"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0823067.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(4090..4752)
                     /locus_tag="ACG8Z3_000726"
     CDS             complement(4090..4752)
                     /locus_tag="ACG8Z3_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0823068.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4936..6582
                     /locus_tag="ACG8Z3_000727"
     CDS             4936..6582
                     /locus_tag="ACG8Z3_000727"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0823069.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            6573..7655
                     /gene="uxuA"
                     /locus_tag="ACG8Z3_000728"
     CDS             6573..7655
                     /gene="uxuA"
                     /locus_tag="ACG8Z3_000728"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0823070.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7763..9316)
                     /locus_tag="ACG8Z3_000729"
     CDS             complement(7763..9316)
                     /locus_tag="ACG8Z3_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823071.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            9464..10632
                     /locus_tag="ACG8Z3_000730"
                     /pseudo
     CDS             9464..10632
                     /locus_tag="ACG8Z3_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(10758..11837)
                     /locus_tag="ACG8Z3_000731"
     CDS             complement(10758..11837)
                     /locus_tag="ACG8Z3_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0823072.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(11940..12380)
                     /locus_tag="ACG8Z3_000732"
     CDS             complement(11940..12380)
                     /locus_tag="ACG8Z3_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0823073.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(12437..12847)
                     /locus_tag="ACG8Z3_000733"
     CDS             complement(12437..12847)
                     /locus_tag="ACG8Z3_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0823074.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(13027..13461)
                     /locus_tag="ACG8Z3_000734"
     CDS             complement(13027..13461)
                     /locus_tag="ACG8Z3_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0823075.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(13561..14409)
                     /locus_tag="ACG8Z3_000735"
     CDS             complement(13561..14409)
                     /locus_tag="ACG8Z3_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0823076.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            14798..16564
                     /locus_tag="ACG8Z3_000736"
     CDS             14798..16564
                     /locus_tag="ACG8Z3_000736"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0823077.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            16666..17925
                     /locus_tag="ACG8Z3_000737"
     CDS             16666..17925
                     /locus_tag="ACG8Z3_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0823078.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            18072..19373
                     /locus_tag="ACG8Z3_000738"
     CDS             18072..19373
                     /locus_tag="ACG8Z3_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0823079.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            19376..20227
                     /locus_tag="ACG8Z3_000739"
     CDS             19376..20227
                     /locus_tag="ACG8Z3_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0823080.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            20240..21055
                     /locus_tag="ACG8Z3_000740"
     CDS             20240..21055
                     /locus_tag="ACG8Z3_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0823081.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            21206..22372
                     /locus_tag="ACG8Z3_000741"
     CDS             21206..22372
                     /locus_tag="ACG8Z3_000741"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0823082.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            22602..23249
                     /locus_tag="ACG8Z3_000742"
     CDS             22602..23249
                     /locus_tag="ACG8Z3_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0823083.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSETSTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            23333..24277
                     /locus_tag="ACG8Z3_000743"
     CDS             23333..24277
                     /locus_tag="ACG8Z3_000743"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0823084.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            24412..25863
                     /locus_tag="ACG8Z3_000744"
     CDS             24412..25863
                     /locus_tag="ACG8Z3_000744"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0823085.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            25884..26720
                     /locus_tag="ACG8Z3_000745"
     CDS             25884..26720
                     /locus_tag="ACG8Z3_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0823086.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            26720..27379
                     /locus_tag="ACG8Z3_000746"
     CDS             26720..27379
                     /locus_tag="ACG8Z3_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0823087.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            27505..28194
                     /locus_tag="ACG8Z3_000747"
     CDS             27505..28194
                     /locus_tag="ACG8Z3_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823088.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            28200..29156
                     /locus_tag="ACG8Z3_000748"
     CDS             28200..29156
                     /locus_tag="ACG8Z3_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0823089.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            29200..29712
                     /locus_tag="ACG8Z3_000749"
     CDS             29200..29712
                     /locus_tag="ACG8Z3_000749"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0823090.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(29968..30516)
                     /locus_tag="ACG8Z3_000750"
     CDS             complement(29968..30516)
                     /locus_tag="ACG8Z3_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0823091.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(30605..31786)
                     /locus_tag="ACG8Z3_000751"
     CDS             complement(30605..31786)
                     /locus_tag="ACG8Z3_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0823092.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(32022..33530)
                     /locus_tag="ACG8Z3_000752"
     CDS             complement(32022..33530)
                     /locus_tag="ACG8Z3_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0823093.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            33689..33880
                     /locus_tag="ACG8Z3_000753"
     CDS             33689..33880
                     /locus_tag="ACG8Z3_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823094.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            34083..35000
                     /locus_tag="ACG8Z3_000754"
     CDS             34083..35000
                     /locus_tag="ACG8Z3_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0823095.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            34993..36066
                     /locus_tag="ACG8Z3_000755"
     CDS             34993..36066
                     /locus_tag="ACG8Z3_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823096.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            36214..36732
                     /locus_tag="ACG8Z3_000756"
     CDS             36214..36732
                     /locus_tag="ACG8Z3_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823097.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            36713..37105
                     /locus_tag="ACG8Z3_000757"
     CDS             36713..37105
                     /locus_tag="ACG8Z3_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0823098.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            37181..38116
                     /locus_tag="ACG8Z3_000758"
     CDS             37181..38116
                     /locus_tag="ACG8Z3_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0823099.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            38249..39028
                     /locus_tag="ACG8Z3_000759"
     CDS             38249..39028
                     /locus_tag="ACG8Z3_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0823100.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            39021..39338
                     /locus_tag="ACG8Z3_000760"
     CDS             39021..39338
                     /locus_tag="ACG8Z3_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0823101.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            39582..41192
                     /locus_tag="ACG8Z3_000761"
     CDS             39582..41192
                     /locus_tag="ACG8Z3_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0823102.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            41349..42197
                     /locus_tag="ACG8Z3_000762"
     CDS             41349..42197
                     /locus_tag="ACG8Z3_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0823103.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            42428..42907
                     /locus_tag="ACG8Z3_000763"
     CDS             42428..42907
                     /locus_tag="ACG8Z3_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0823104.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            42940..45168
                     /locus_tag="ACG8Z3_000764"
     CDS             42940..45168
                     /locus_tag="ACG8Z3_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0823105.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            45297..46940
                     /locus_tag="ACG8Z3_000765"
     CDS             45297..46940
                     /locus_tag="ACG8Z3_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0823106.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(47013..47558)
                     /locus_tag="ACG8Z3_000766"
     CDS             complement(47013..47558)
                     /locus_tag="ACG8Z3_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0823107.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(47581..48066)
                     /locus_tag="ACG8Z3_000767"
     CDS             complement(47581..48066)
                     /locus_tag="ACG8Z3_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0823108.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(48056..48868)
                     /locus_tag="ACG8Z3_000768"
     CDS             complement(48056..48868)
                     /locus_tag="ACG8Z3_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0823109.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(49007..>50116)
                     /locus_tag="ACG8Z3_000769"
     CDS             complement(49007..>50116)
                     /locus_tag="ACG8Z3_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0823110.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        16572 a         9120 c         9642 g        14782 t
ORIGIN      
        1 tgttcaatca aaacaagcgg taaagaagca ttgctagaag cttcccccgt tagaaaagat
       61 aggctgaaac ttagaactaa aacgactaaa atgtcatcta ccacagaagc acccaaaatt
      121 gttgatcctt cttttgtatt cactacgttt agttctttta atacttctac tgaaatactg
      181 acagaagttg ccgcaagaat aatcccaaag aaaatcgctt catttcccga aacttggaat
      241 gctttgcctg tcaaccagcc aaaaacaacc gggaagatga tcccaagcaa agcaacgaac
      301 ataccaggtc ggaaatattt tttcaaaaga gataaatcac tttccagacc agctaaaaac
      361 attaataaga tcacaccaat ctccgaaaag tcatggacta gaatatcagg tttgacccaa
      421 ttcaatccag cttggcctag caaaacacca acaagtaatt gtccaatcac tgctgggata
      481 cctaatctcc gcgagatatg tgagccaatc gttgttgaaa tcaatatcag gcataaaata
      541 ccaataaatt ccattctcat cattcctttg tataaaatat agcccgaact tagcataaca
      601 tacattattg aaaaaatagg aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt
      661 tttggattgt tggtttttat acggtctatt ttaataacta aaaatgatta acgtatagtc
      721 atttttctta ttctgatgaa attgataatc tcctggatgc ccatgaaaat caaaataaag
      781 ccgaatattt ggaatagaag catcaaactt ttgaatggat tgaacaataa gacgaatccg
      841 ccaatcaaca acagaatact actataaaca ctccaacttt tagcttttgt attcatgctg
      901 aagaacaact ggaacaagct gttgatgata acagctaatc caagaagaat cggcaggata
      961 gaaacgattg caggtgcaaa agcatagacg atcagagcta agatcaggaa aaaaagacca
     1021 ctgaacagac cgatactcca agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga
     1081 ttcaacaacc caagcaaaac aagataggcg gtgatcagat aaccgataaa atgaaaaaca
     1141 gcagaagggt ttagcacaat cgcaatccca gaaataatat aaacagcagc tcttaataag
     1201 gcgtattttt gaatattttg tttaatcgtc tcaaacatat aagaaaacct cctttgactt
     1261 attttaacgt gaactagtca ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag
     1321 aatatctctg gaaaaatcaa cttctgagga tcttttccat ttttttgcct tttgctagct
     1381 catcgatcat tttatctaaa taacgaattt ctttcatcaa gttatcctcg atgtcttcta
     1441 ctcgtacacc acaaacaaca cctgtaatca aggaacgtga aggattgggg acaggggctt
     1501 ccgcaaaaaa ttgttcgaaa gtcacttgtt tttgtagttg atattctagt tctgtttggg
     1561 aataacccgt cagccaacaa atgatttcat ctacttcctc ttttatacga tcttttctct
     1621 ccgcttttgc tacatacata ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt
     1681 gcgccataat cagtcacctt tctttttttc aaattattta ctacttatag tatataagaa
     1741 aaagagaaga aaaagcgatt attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca
     1801 ccatcctttt ttccgctctc tgacttttta caatcttcat agctcaagtg cgcatcagat
     1861 aatcgaatgc tcctaaagct gcagtagctc ctgaacccat tgaaacaatg atttgtttgt
     1921 atggactatc tgtacaatca ccagctgcga aaacgccggg gatattcgtt gcgccatttt
     1981 tctcggtcac tatttcattt cttggattca tttcgatcgt tccatctagc caatccgtat
     2041 tagggagtaa gccgactaga atgaatactc ctgaaacagc tagtgtatgc atctccatcg
     2101 tacggcgatc gatataggag agagattcta cctgattcgt accgctgatt tctttcgtcg
     2161 ctgcatgttt gataacagtg acattcgcaa gagagactag tttatcctgt aagatttgat
     2221 ctgcttttaa ttctggtaag aattctagaa catatacatg ttttgctaac ccagccaaat
     2281 cgattgctgc ttcaacacca gaattaccac caccgataac agcaatctct tttcctttaa
     2341 ataaagggcc gtcacagtgt gggcaataag caattccttt atttttaaac tctttttcgc
     2401 ctggtacatt gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa
     2461 gtgatatacc gttagctaag ccaatattaa taaaatgttg ctctttattt aaggaaacgg
     2521 cctgttgatt catcataatg tcgatagggt aactacgcac atgactttct acttgctgca
     2581 tcaattttgg accttctgtg taagaggtac caatcatatt ttcaatccct agtgtttcaa
     2641 cgacttgtcc gccgaaagaa tcagcgacaa gtcctgtacg gattccttta cgtgctgcat
     2701 agatagctgc actactagct gctggtccac cgccaattac taatacatca taaggttctt
     2761 tttcaaacaa ctcttcttca atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg
     2821 tcatgcgtcc gctactaaat tcttctccat ttaaaaacac agttggcact gccatgattt
     2881 ttttatcatc gatttctttt tggaacatcc caccttcaat catcgtatga gtaatatttg
     2941 gatttaatac agctaatata ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca
     3001 agctaacata actgtcaaaa tgcaatgttt gtgttatttt tttgatttta agactcaaag
     3061 attcactgat tttcggtgcc cgtccactaa tttgtaacat agccagtaaa aaagaggaaa
     3121 attcatgacc gagggggata ccagcaaaaa cgattccact ttctttgcca actggattga
     3181 tagaaaaact aggtgtgcgc atcgaatctc ttttttccca agagatttta ggagacattt
     3241 ctgctatttc cttcgtaaat tcagctagtt tttgactatt ttcactatta tccaaactaa
     3301 cagaaaaaac aataggagat tcaatcagtg ctaaatattg ttcgagattt tgtttagttt
     3361 ctttgtctaa catatccacc acctaaatct ttccaacaag atcaaaactt ggtttcaatg
     3421 tttcgccact ttctttccat tttgccggac aaacttcacc tgggtgggta cgtacatatt
     3481 gagcagcgcg aatcttatca atcagtgcac tagcgtcacg accgattcca tctgcattga
     3541 tttccatagc ttgaacgatt ccgtctggat caataataaa tgttcctcgc tgagctaatc
     3601 cttgctcttc atccaaaaca tcaaaaatac gagaaatctg atgagacgga tcaccgatca
     3661 tcacgtattc gattttaccg atagcatctg atgtatcatg ccatgctttg tgagtaaagt
     3721 gggtatcggt agagcaagaa aagacttcaa cacctaaaga tttcaatgta ctgtattgtt
     3781 cttgtaagtc ttctaattca gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa
     3841 gaatactcca gttccccata agatcttttt cactgacttc taaaaattct ccttgatgat
     3901 aagcatgtgc attaaattct tcgatttttt tcccaatcaa tgacatagta aaacctccaa
     3961 taataattat ttatttataa ttattgtaat ttagttggag ttgcaaagtc aatggatatt
     4021 ttgttattta ataaagatta ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc
     4081 aagaaaatgc tactgctgga aatactcggg ataacgttga gtaacagttt cctgttcttt
     4141 cagcatcatg tgtaagtgca atcccgtttg aaatcgagct tcttctggtt ttttatcaac
     4201 taaagccttg agtaaatcat aatgttgttc catgatcgtt tcccatggaa gcccctgaat
     4261 ggtcactcgt aaccagcgga aacgttctaa atgggtatta tggtcatcta accagttcca
     4321 aacttctttt cttcctgcaa tttcgaaaca agcttcatga aataaattat ctgcacggaa
     4381 aaaagcgcgt tcgtcattta ttttgactgc tttttcttgt tcttctaaaa tcgtttctag
     4441 cactattcgt gtttgtgtgt ccaatttgga acaacattca atcatgattt gctcttccaa
     4501 ctgttctcgg acaaagcgag cattttctgc agaaacaaga ttaattttgg aaacatacgt
     4561 accactttga ggtatcgtat acacgagttc ttgctgacgt agttgaatca atgcttcgcg
     4621 aatgggagtc cggccgatag caagatgttc ctccagtgcc ttttcagata ttttccttcc
     4681 aggttctaag tcagcataga taatttgttt acgaatcgct tgataagctt gtgattgcaa
     4741 gctgcttttc atctttattc ctccaaaatc atttctatga acgtagtata acagaagagg
     4801 tctgaaaaac aatctggtat gtcacattaa ctagtaaatt tgtataaata gtaaaatttt
     4861 ggttgacata gcgattttta ttgtataaaa ttcacttgaa tacaaaaact aatatgttag
     4921 tttaggaggg gaaaaatgat tgatttaaac aactggagga actttatccc tgaattatct
     4981 caaaaagaag tagaaatacc tcattttgac cgtgcatcat taagagaaaa tggacaaaaa
     5041 aatcctgtct ggatccattt cggtggcgga aatctttatc gaggatttca tgcagaaatc
     5101 gctcaacgtt tagcggatct aggcgaattg tccactggtg tcattgtttg tgaaacattc
     5161 gatgaagaaa taattgaaag ggcttaccaa gattttgatg atttgattct tgaagtggtc
     5221 atgccaagca gtggagagtt cgtcaaacgt atattagctg cgacagctga cagtctgtat
     5281 tgtcatccag attgttctga acactttgat aagatgattc gacactttga aaatcctagt
     5341 ttacaattag ttacttttgc tattactgaa aaaggttatc gattgaaaga tacaaataat
     5401 aaatatatag gaccgattgc agtcgatata aaaaatggtc caaagaagcc atcacataca
     5461 atgagtattc ttgctgcgct actttataaa cgttttttat ctggagcttg gccgattgca
     5521 cttgtctcta cagataattt ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg
     5581 atcgcaaaag aatggcaaaa ggaaggttat gtacaaaata catttatcca atacttatcg
     5641 aatgaagaaa ttgttgcgtt tccatggacg atgattgatc gtatcacccc gaatccttcc
     5701 ttgtcagtta gcaaaatgtt ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc
     5761 gcaaaaggaa ataatattgc tccatttgtc aatacagagg acatccatta tttaatcatg
     5821 gaagatcatt ttcccaatgg acgtccgaaa ttcgaaaaag ctggggtatt cttgactgat
     5881 cgtgcaacgg tagataaagc agatacgatg aaggtgacga cttgccttaa tcctttgcat
     5941 acgtctttag ctattatggg ttgtttactg gggtatagga gcatagctga ggagatgaaa
     6001 gatccagatt tatcgctgct agtttctgaa atcgggtaca atgaaggcct cccagttgtg
     6061 gagtctccag gtatcattga tccaaagatc tttctggaag aagtaattac tgtgcgcttt
     6121 tctaatccaa atattcctga tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg
     6181 gcgattcgtt atggtgagac gattaaaaaa tatatggaaa tagaagttaa agacccgcag
     6241 acacttgtat tcataccatt gactctcgct gcttggcttc gataccttat tggcattgat
     6301 gatcaaggaa aaccattcat cccgagtcca gacccactat tgacagactt tcagcataag
     6361 ctttccgata ttcaactggg tacgcaagat acatcacata ttcatgagaa aatggctcct
     6421 attttgtcaa ataaagaaat ttttggtgta gatctttacc aagcagagct tggcagaaaa
     6481 atagaagaaa tgttcatcaa gatggtagca ggaccgaatg ccgtacgaaa aacattacag
     6541 gattacttac aagaacatgg aggaaattta tcatggaaat gacttttaga tggtacggat
     6601 cacaggaaga aaagattacc ttagatgaaa tcagacagat tcctgcggtc aaagggattg
     6661 taggcacatt gatggatata ccagtaggag aggtatggcc cagggagagg atcaaagaac
     6721 taaaagaaga aattgagtct cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc
     6781 atgatgccat taaaattgga ttaccttccc gtgagcagta tatcgaaaac tataagcaga
     6841 caatccgaaa cttggcagaa tatggaatag aagtcatctg ctataatttt atgcctgtat
     6901 ttgactgggt gaaatctgac cttgactacc gcttagaaga cggctcatcc acgttagctt
     6961 ttatatctga tgatattcca gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca
     7021 gcaatgaatt tgaattgccg ggatgggaac ctgagcgctt agctcatatc aaatccttgc
     7081 ttgaagccta tgcatcagta gacgaagaaa aacttcgaga aaattttgcc tattttctcc
     7141 aatcaatcat cccaacatgt gaagaggtag gggtcaaaat ggctgtacat ccagatgatc
     7201 cgccatactc gatttttgga ttacctcgaa tcgtaaagaa tcgagaagat ttagattggt
     7261 tgtgccgcat agtagacagc ccttataacg gaatcactct ttgtacaggg agtattgcag
     7321 aagatccaga aaatgatgta tatgggattt tagcagaatt ttgtcaaaga gatcgaatcc
     7381 catttgcaca tgttcgaaat attaaatttt tagctaatcg agatttctat gaggcaccgc
     7441 ataaatccga atacggttca ttagacatgt accgcatact aaaaacaatg tatgacaacg
     7501 gatttaacga atacatccgt cctgatcatg gaagaatgat ctggaatgag acaggaagac
     7561 caggatacgg cttatatgat cgagcattag gtgctgttta tctaaatgga ttatgggaag
     7621 caatcgaaaa aggaaacaaa agtaaagata attagtgaaa aaaactgtaa ttttattatg
     7681 taaataagaa ggatgttcaa attcttgata cataaatccg gaggttgtta ataagcattc
     7741 ggatttatgt atcttatttg gtctaggaag taacttgatt ttcttttttc gattgatcca
     7801 tgagtgcttc ttcttttaat tcaaagatat attctgagat cctcataatc acttttttcc
     7861 agtcttctgg tgtgatgatt ccttttaaaa tatagatttt tttgatatga gaaggcaatt
     7921 gtttcaaagg gcagtcgctg ataacgatgt tgcagttctc cataaaatca ttttgtattt
     7981 tgatcgattc atttgaaaaa atttgttcta tcttaacgat cagatcttta ctgatataaa
     8041 aatcacgcgt atagttcaca ccaatcacaa ttcgcggctg ttttttgtag cgatcataca
     8101 aatgtactag gtcttcaacc atccattgca cattttcctt ttctaaaata actggtaaca
     8161 agtgttgatt ttgttctctg aaatccatat aaaattgttc aatggcggtt tggacttctt
     8221 tcaacacgct atgttcttga taaagcatga aagtaggaag gggcgtattt ggttgattga
     8281 agttaagaaa tccattataa agtttatttc gtgcgaatgc caatgagtaa agaatctttt
     8341 ctttttcagg tataaacaaa tcaaatacag tacaaaagga ctccaccatt tctcttgaat
     8401 aaagaacatg aggaacgttt gcttttttta aaagtttgta ttgctccaag cttgtttctt
     8461 ccgattcgat tttcgtgata gaaagtcgag ctaaaatatt cactaagatg cgttgttcgc
     8521 gacaaattgg cagattaggt cgcaaaatat ctacagaggg atcaataaaa aagtcaatat
     8581 attcatactc tgtgaattct ttttgtacgt ctttcacaga agttgcagga aaattctctt
     8641 tacatatttt taatagaaaa tataatttat ctatcatacc gctatttagt cggtcaagta
     8701 cttctttttt aaaaaaagta ttcatcataa aagccactac ttgcggatct tcatcataaa
     8761 attctgtagc agtacttgaa aagatgttcc aataaatatc taatgctgca tattgaattt
     8821 gtacctcttt tccagaaaaa gactttctgc cattattaga aaaggagata gacaaaccgt
     8881 acaatgctaa aaatgcatta atctttttca ttttcgtata caaataagag gcactatagt
     8941 ttgtttgctg acttaaactg ataatcgaat ttgtctgaca gctgaacaaa gaagtcaaga
     9001 tctgataact gactgattcg ttgcagtatc ttccaaatag acgataatag cagtaagccg
     9061 gatcaattcc ttcaaaattc aactttaaaa aaggtttttg cttatcaata tgcagttctg
     9121 ttagattgag acgctcaaag tcttgttcta attctttgat gtatagtaaa aaatttgatt
     9181 cagacatatg gaaatgatct gctaaatatt gtgtagtaat aaagaaacga tgttcagata
     9241 ggaaaagaaa aatatctaat tttgtattgc tttttttctt caagagaaac gttttaatcg
     9301 cttcttgagt ttgcatgaaa gaggctcctt tttacttaaa ttgttaaaac ttgaaaatat
     9361 tactgttttt ttcataaatt tagacaaaaa aacagtaaac atataggtag aatctctact
     9421 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
     9481 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
     9541 agaggagata gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
     9601 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
     9661 agcgaccatc aaagcaaaaa ttgactattc aggagactta gcacctatac tacaatcagc
     9721 tgttacattt gagaatgttg atcccgcttt gtcgattggc tatgacgaga acactttgac
     9781 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
     9841 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atcatttaac
     9901 gccgtatagt cagcggcaga caattgagca agattcagct atcgactcag gaggagatct
     9961 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
    10021 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
    10081 tcaaccagag ccagacaatg aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
    10141 agacccagaa gaaaaaccag atgtgactcc agaaccagat acggattctg gaaaccaaac
    10201 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aaccagaggt
    10261 agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg
    10321 aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa
    10381 atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt
    10441 tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctgtcct
    10501 gaaacgaaaa ataaacagct accaaaatcc ggagagtcac aaaacaaagt tatactgtgg
    10561 agcggaatca ttctattaag tatagcaaca atgctttctg caaaaagatt taaacaaaac
    10621 agatctctct aacatttgaa taatgtctgt gaacgcattt ttcaaataaa aagaaatatc
    10681 cagaaaatga ccccaaaata ccagattgaa atctgatatt tggggtcgtt catactttat
    10741 ctttcttcat atttatttta agggatatct gtttcttttg gaagatggtt cgggatcttt
    10801 ttcactttca atacatctaa aaagaaggta aagatcggaa tgccgacaat caagccccaa
    10861 acgccaaaga accgttcgct aacaagtaag accacaaatg tataaaaaat cggcagttct
    10921 gttttgctgg acatcaactt cggattcagc acataagact caattagatg gacaatcaaa
    10981 atcgtaatca ggatataaac aacatctcgg atgcctcctt gggaataagc tatgaaagat
    11041 agaggaatac aagaaatgat aacacctgct actgggatca gacttaaaat gaaaatcatg
    11101 atcgctaaac tcagcagctg atgaaagcca aatacagcca aggcgattgt agtgagcaac
    11161 gtatttaaaa gagcgacaac aaattgagct tctaatacca aaccaaatgt attgacaaac
    11221 ttatcggcaa aatagtaaat atcttgaaag aaccaagaaa attcactttt taaaaacaat
    11281 cttgagaata caattgtttt tttcttttca atcataaaaa agaaacttaa gatgaatgac
    11341 atagcaaacg ccactgccat ttttcctata tcctgtaaat agccaagcgc aatcgaagcg
    11401 ccattttgaa gctgttcaaa aaaatcgttt cgctcaatat aagtatggat atattgtaaa
    11461 acaggatctg catcttttgg ctgatgctcg taaaaattaa taacggactg gatcatgtca
    11521 aatgtttgat gaataagcat cgggatatac tttgtgaatg ccagatagat aaggtaaacg
    11581 atcagtgtat aaatcatgat agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg
    11641 atttgaacaa gatgaatcat caaataagta aagataaatg tgagcaaaat ggttgtaatc
    11701 atacttctaa ctaagaaaag aacaaaaacg acaaataata aaacagtgag acggcgtatt
    11761 ttttcattcg ccaaaaaacg ctgataaacg gtcaaatcga caacctccaa ataacaaaaa
    11821 ataaacacgt tattcattat atgatggaat atgaaaataa gaaagagcaa aaagaaaaac
    11881 cgctaaatga cgttatctta tgagttggtt ttcctcataa aatcgttcat ctagcagttt
    11941 cagccctttt tctggtcttg atgaaaatta tctagcattt ttcgggtcag atgttgcagt
    12001 gtatccaatt cttcctgttc aagtggaaga taagacttga tcttttgtgg aataccttct
    12061 gcttgttttt gcaacgcagc accttgagca gtcagcgtaa catttacgat tctttcatca
    12121 cccgtttcac gctgacgatt aatataattt ttttctgcta acttttttag taagggagtc
    12181 agcgtaccag aatccagaca caaatcattt cctagctctt tcactgaagt ctttttcttt
    12241 tcccataaaa ctaacatcac aagatactgg gtataggtaa gattcagcgg cttgaggagg
    12301 ggagtgtagt gtctaatgat ttctttagag gcggcatata aaggaaaaca tacttgatta
    12361 actaaatata gatcttccat ttttccacct ccgctgtttt actatagtgt atcatagcgg
    12421 atttacacaa aaaagtctat tcactaactg ctttcagagt aacttctata tttccagctg
    12481 tagcttttga atacggacac actttatgcg ctgcctctaa taattcttgc gtttcttcca
    12541 gagaaagacc ttcgatagat ccttcaatct ctacacctaa gacaaagctc gtttcacttt
    12601 ctgaataaag ggaaacttgt gcactgacag tagaagcccc agagatattt ttttgctgtt
    12661 tgatcaattc taatgcactg ttaaaacatg cactgaaacc agctgcaaac aattgttcag
    12721 ggttggttgc attttcgacg cgtttaccag gagctgcaat attcaattcg aatgtacgat
    12781 cgggattatg cacttctcca gagcgtccgc ctgtgttgat cattttcgta gaatacattt
    12841 ttttcataaa attaccacct ttaattatat tttatacaat tgaattttac acaatataat
    12901 tgtggaaagc aaagaaatgg attggcataa gataaataag agaaagcaca tgaaacagac
    12961 gcgctttctc gaatagcaaa catatacaaa aaatatcaga gacaaaaaat aatctcatca
    13021 taaacattac atatatttct caaaaagttg ttttctttct tcaaaaatta cttcacgatg
    13081 gacctgatga agcatccata aataaccaaa aggatctctg aacattgagt tagtcacacc
    13141 aaagtccatc atttctgtca tttcttgaat ctctacacag ccatttcgta atgctttatt
    13201 atgtgtgtcc tcgatatcag agacgatgat attgaaccag atcgtatttg gatgttcagc
    13261 agtaggagca gtcaaaccaa gagacgtgtt ttcatccagc atatggaatc tcgtaccata
    13321 caaggtaaaa atgacttcat tattgcctct agggtaatct gttgcctcaa tcagttgtat
    13381 ttcatcaaag attgtttgat acagttctag tgctttcaaa ctatcattta atacaaaatc
    13441 gatttccgtt cctttatgca taaagatctc ctctcctttt cagtcgaaca gttactagat
    13501 tcttttatta taccagctaa agaaaacgca ttcaaaaaaa tagcggattt acttaccaac
    13561 tcattcgatt ggatgataag taaatccgcg tcccattact tctgctacgc tgctgacaat
    13621 gacaaaggca cttggatcga tttcttctat caatggtttc attcgggaaa attcatgatc
    13681 agtcacaacg atcaataaaa cctcttttga ctttcgatta tacccgcctt ttgcctctag
    13741 cagggtaagg ccacgtccaa tctcagcact taagcgaagg cggatgtctt ccaaataatg
    13801 ttcactcatg accataacag atttctttcg attgatacct gtttcgatat aagtcatgac
    13861 aattgaagtg attgcaatag ataaaatagc ataaagaaac tcttctattc caaaaataaa
    13921 cagattaaga gaaacaacga atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg
    13981 gaaatatttt ttaaaaatga ggggagggat cgtcgtaccg ccgctagaag agttgttttt
    14041 atataaaatc gcaactccta aagcaaaaat cgcacttcca aaaatgacag acaacaaccg
    14101 atctgaagta atcattgttt ctggaactat tgcaatgatt aaaggaaaaa ccaagcttcc
    14161 gaaaagtact ttaaaaaagg gttttttacc taaaaataga agagctagta ccaacataac
    14221 gatattcaac actaaaataa ctatagctcg gtcaaagcct aaagcagctt ccagtaatat
    14281 tcctagaccg ctgacacctc cagcagcaat atgatgaggc cctaaaaaca tattgatagc
    14341 gactcctaat atgaagaggg caaaaataat cttaatgtat gtccatataa attgtctcgt
    14401 ttctttcatt tattcacttc cttaattcat taataatact cttatcttat tataacttac
    14461 ttttcacctt aagttaactt taattaataa aaaaattagg ctagtctaaa ttattatgtt
    14521 tcattcttct agaatatact tctacgccaa aaactgttaa aaaactttta ctttagaaaa
    14581 ttggagaaat tgatcgctta agataaattc aggacactaa atagtacagg aagtagacta
    14641 aaacttttaa aagttttaag tctatctatt tggcaagttt aaaaaataaa aattcggtat
    14701 caaaaaactg ttaccgataa catttttgaa agcgttgtct tttgcttctc tatcacttat
    14761 agtgatgacg tcatattaaa acacaaggag gaaatcaatg gatacagctg caatttatca
    14821 tcgaccggaa agtgaatatg catttctata tacgaataca agatttcgta ttcggcttcg
    14881 tactaggaaa aatgatatca aaaaagtata tgtgctatgt ggagatccat ataccataac
    14941 aactgaaaaa tggtatcaaa aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca
    15001 tgattattgg gaaattgaag tgacgagcga aacaagtcgc ttacagtatg cgttccatgt
    15061 tgttggggaa gataacacgg actgttttta cggtgatcaa ggaatttttc cttatcgaga
    15121 aaatgtgatt actgaaccaa atttttattt tcgaatacct tattttcatc aaatcgatcg
    15181 attcactatt ccagaatggg taaaagaaac agtatggtat caaatttttc ccgaaagatt
    15241 tgcaaacggg gacaaagcaa atgaccccga acacacttta ccttggggaa gtaaagatcc
    15301 agaccgagaa gactttttcg ggggagattt gcaaggtgtg atggatcatt tggattactt
    15361 gacggacctt ggcatcaatg gcatctattt ttgtcccata tttaaagcca catccaatca
    15421 taaatacgat acgattgatt attatgaggt ggatcccgct tttggcgata aaaaactttt
    15481 aaaaaacttg attgatgaag cacataaaag agggatacgt atcatgttcg atgctgtatt
    15541 caatcatatg ggtgtccatt caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc
    15601 gatctataaa gactggttcc acatccgttt tttcccagta gattcttatc agatgactga
    15661 cttaccagaa actgctgaaa atatcccata tgatactttt gcttttactc cattcatgcc
    15721 gaaactaaat acagcaaatc cagaagtgca aaaatatttg ctggatatcg caacttattg
    15781 gataaaagaa ttcgatattg atggatggcg tcttgatgtt gctaatgaag tcgatcatca
    15841 tttttggaaa aaattcagag aagcagttac agaaatcaaa ccagacatct atatccttgg
    15901 agagatttgg cattcctctc aagcttggct gcaaggggat gaattccatg cagtgatgaa
    15961 ttatgcattt acagattcga ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca
    16021 aatggtcagt ggaatgaatc atcagcaaat gctttacaga gatcaggtga acgaaggaac
    16081 attcaactta ttagattctc atgatactgc caggatcttg accctttgcc aaggtaacaa
    16141 agaattgatg aagtctgtga tggcctttat gtttcttcaa aaagggtctc cttgtatcta
    16201 ttacgggaca gaaataggga tgactggtga agatgacccg gattgccgca aatgtatgat
    16261 atgggagaag gaggaacaag atctagagct attcggtttt ataaaagaac ttgtttctct
    16321 aaggaaacag ttgtctaaga ttatttctga aggttctact cagtggctaa tagtaaatga
    16381 tcgtgaagat aagctctatt tcacacggaa actagaggga cagatcatct atgtttactt
    16441 taatcagtca aaagaacctt gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa
    16501 ttgtcagttg ttagaagacg ggaaagcaga gatacgacaa tacggatttt tgatcatgtg
    16561 ttgaaaactg ttaccggtaa caacaagaaa gcgtttgcaa atgaagattt gtgcccatta
    16621 taataagggt gtaacagcac gttgaataca aaggaggaaa tgaagatgaa aaaaagaagt
    16681 gtgaaacagt tgggtttagg gttaatctct ttaggaatct taggcggatt agcggcttgt
    16741 ggcaatggta actcttcagc agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc
    16801 gttgattctg gatataaaga ttatataaac gaaataaaaa acactttcga aaaagaaaac
    16861 gatgtgaaaa tcaagctagt agaaaaggac atgttcgatc agttggaatc attagcgtta
    16921 gatgggcctg ctggaaaggg acctgacgtt atgatggcag catatgaccg gatcggtgcg
    16981 ttaggacagc aaggacatct ggcggaagta aaactgggaa atgaagcagc ttacgatgaa
    17041 acagacaaag cccaagtgac ttatgatgga aaaatctacg gagaacctgc agtaatcgag
    17101 acccttgttt tgtactataa caaagattta gtcgatacag caccatcaac gtttaaagat
    17161 ttggagaatc tatccaaaga cagtcgtttt gcttttgagt cagaagcagg aaaaaataca
    17221 ggatttttag ctaaatggac tgacttttat tattcctatg gtttaattgc aggttacggt
    17281 ggctatgtat ttggtgatga tggcacaaat ccttcagata tcggtttgaa taatgcaggt
    17341 gctgtagaag gaatctctta tgcaacagat tggttcaaaa atgtttggcc aaagggaatg
    17401 caagacataa aaagtgcagg agacttcgcc agccagcaat tcatgtccaa taaaactgca
    17461 gcaatcattg acggtccatg gcaagcgcaa acatacaaag aaaacaatat caattatggt
    17521 gtagccaaaa ttcctactct aaataatgga cagccttatc aaccgtttgg cggaggaaaa
    17581 ggatgggtag tcagcaatta tgctaaaaac aaagacctct ctcaaaaatg gcttgattat
    17641 gtaacgaatc aggaaaatca agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac
    17701 caacaagcta gggaaacagc aaaggctaaa aacgatgaat taacaacagc tgtgattgaa
    17761 caatatgaaa cagctcaagc gatgccaaac atcccagaaa tgggcgaagt ttggacaggt
    17821 gcagaaaact tgatgttcga tgcagcatca ggttcaaaaa caccaaaaga atccgcagat
    17881 gaagcagtaa aaacaatcag tgaagccata gaacaaaaat actgattaaa atgataaccg
    17941 tccattttta taaaaaatgg aacaggtgct tcagataaaa gtggcaagag cgagatggaa
    18001 aattatcttg ctcttgttgc cattatctta aaagcagttt gcggtttagc aagtttagag
    18061 ggggcattca gatgcagcaa gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt
    18121 gttcacttat tccagggatg gggcagtttg taaatggcca agcttttaaa ggtatcgttt
    18181 ttttcgccat attcgtccta ttcattggtc agatgcttct gggcggattt catgcattgg
    18241 taggattggt tactttggga agtataccaa tggaagatca ttcattattt attttgattg
    18301 aaggcacttt gcaattcatc attacaatca ttttcttgat tttttacggt ttgaatcttt
    18361 tagacgctta tcgagtagcg aagatgcgtc aagagggcaa aaaaatcaat cggacgatga
    18421 aggaagtcat acataatata ggtgatgaag gttttcctta tctattgaca cttcccgctt
    18481 atcttctcat gatttttacg atcattttcc cagtcctagt tacattgttc actgcattta
    18541 ctaattatga cttcaatcat attccaccag ccagtttaat tgattgggta ggggctaaaa
    18601 acttttttag tatcttcttt ttaagctcat atcgtaatac tttttttgca gtcttttcgt
    18661 ggaccgtgat ttggacttta tgtgcaacaa ctttgcaaat cacccttggt attctcacag
    18721 cggttgttgc tcaccagaaa tttattaaat tcaaacgtat tttcggtatc attttcttat
    18781 taccatgggc ggtcccagct tttattacta tcatgagttt ttcgaatata ttcaacgata
    18841 gcgtaggtgc catcaatact caagtgattc ctttcataaa ccagcttcct tttgtagata
    18901 tcagtgcaat ctcttggaaa acggacccga actggacgaa agtagcaatt atcttagtgc
    18961 agggctggtt aggatttccc tatatctatg tgatggttac aggaatctta caatcgattt
    19021 cagaagactt atatgaagca gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa
    19081 acatcacgct tcctgctgtc ttcctagtgg cagcgccaac atttattaca cagtatacgg
    19141 ggaatttcaa taatttctcc atgatctatc tgtttaacaa tggcggtccc ggaagcgtag
    19201 gcggaggagc tggttctact gatattctta tttcctggat ctataaactt acgactggaa
    19261 cttctccgca gtattctatg gcttcagcaa tcacgttgat tatctccgtc atcgttatct
    19321 gtgtgtcatt gctggtattt aaaaagacaa atgcgttcaa tatgggggat taacaatgaa
    19381 aaaatttcat tcgagtgctc gattccaaaa acgactttct cactttttta cttacttttt
    19441 tcttttaata ctgtcagtca ttatcatcta tccgcttttg atcacagcaa gtactgcctt
    19501 caagtcagga aatattgcag cttttagttt agatctttca agtgactgga ctttaggaaa
    19561 ctttactcgg ctattcagtg aaacacttta cgggacttgg tataaaaata cattgatcat
    19621 ttctgtccta acaatgatcg tacaagtgac tgtagtaact ttggcgggct atacttatag
    19681 tcgctatcgt tttatcggcc gcaaatcaag tttgatgttc tttttagtga ttcagatggt
    19741 accgactatg gcagcattga ctgcctttta tgtcatggct ttcttattag aagcgctcga
    19801 taagtactgg ttcttgacaa tgatctatat aggcggtgga attccaatga atacttggct
    19861 gatgaaagga tactttgaca ctgtaccaat agatttagat gaatctgcta aacttgatgg
    19921 ggcagggcat ttcaggattt ttttccagat tgtattaccg cttgtcaaac cgatgctcgc
    19981 cgttcaagca ttatgggctt ttatgggacc ttttggggat ttcatgttag ctaagttcct
    20041 gcttcgaaca cctgaaaatc agactgttgc tgtcggctta caaacattta tttctgatgt
    20101 gacaaaccaa agagtctctc tttttgctgc tggggcaatt ttgattgcac taccgatttg
    20161 tctattattc ttctttttac aaaagaactt tgttaccggc ctgacatctg gcggaacaaa
    20221 aggataggag cagagtatca tgcaaacatt tccgttaaac tatttttccg cattacgtac
    20281 gccaactcaa gtgtttgcgg gacgcaaact attatcatgg cctaaatttt tcttgatatt
    20341 tgtattttta gtttccttga tggtcatgcc tgtgactctt ttttatgcca atcagatcca
    20401 agcgattcca ttagaacaat ttctatccgt tcattcgctg atcgatgaac aaggaaccca
    20461 aaaattttct gaacttgaat tatctgaaac tgggttacaa gcatcgcaac aaacgatagc
    20521 tgtgacccct gaaattttgg taggtgtgtc tttatctgag aaacaacaat ccgatcatgg
    20581 aacttttatt gattttgaga aggaacaatg ggtgatccaa caaaaggata aatctggtat
    20641 acgccgctat acgatgaatt atagcccatc tttccaacca gattctgtac gtacgcccga
    20701 agatttccaa cgatttttag aaagagaatt ttatgccagt aatcgtccaa caatcatttt
    20761 atcttatagt ttaagtcttg gtcttgtcct atttgtcatg acgagtctca ttttattcgg
    20821 ggcttccttt ttcttgtgga tgacaagaaa aagccaactt tcatccattc atacattcaa
    20881 agagagtgca aatttgatgc tgaatgttat gggaataggt tccatggcag cagcagttgt
    20941 agggtttatc cactttgact ttatcttgat gctaagtgtc caaacaacga taactgtctt
    21001 gctgctttta tgggtttttg tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt
    21061 ttaagcgatt tatgcgacaa tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa
    21121 atttctgaaa aaattaaact tcttagttga ttcttttctt ttttagtatt aacgtaatag
    21181 ggtagttttt ttaaggagga aaatcatgtt agagaaaact ttatacaatc aattattttc
    21241 acgttctttc tcgctccctg tcgaagtaaa ttattgggac ggtacaacga aacaatacgg
    21301 agatacggac actcctcccc aaatcaaaat caatatccat gaagaaatcc caatgaaaga
    21361 gattacaagt aacgcttctt tggcactagg agaagcctat atggatcatc gaatcgagat
    21421 cgagggaagt atccaagcat tgatcaacga tgcttatcag caagcaaaca gctttttacg
    21481 aggaaaagat tatctaaaat ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa
    21541 agaagatatc catgcgcatt atgacttagg taacgacttt tataaacttt ggctagatcc
    21601 aactttgact tattcttgtg cttatttcaa tacacctgaa gatacgttgg aacaagcaca
    21661 aatcaataaa gtccatcata tcttagacaa actctttatc aaagaaggcg aaacgttgct
    21721 tgatatcggt tgtggctggg gaacattgat gttcactgct accaaagagt ataatgtcaa
    21781 ggcaactggg atcacactaa gtgaagaaca atacgatttg attaccaaaa gaataaaaga
    21841 agaacattta gaagataaat gccgtgtatt attaatggat taccgcgaac taaaaggcga
    21901 aacgtttgat catatcacaa gtgtaggtat gttcgaacat gtaggttctg aaaatcttga
    21961 aggttatttc aaagtggtaa aagatttatt gaaaccaaaa ggaacagctt tgatccatgg
    22021 aatcagccga caacaaggcg gtgcgacaaa tgcttggatc aataaatata ttttccctgg
    22081 cggttatgtc cctggtgtta ctgaattagt tgataatatt acaaaaaacg atttgcaact
    22141 gattgatttg gaaagcttac gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa
    22201 ttttcatcaa gtccaagatc aagtcacgaa agaaatgggt gattccttct atcgaatgtg
    22261 ggatctatat ttacaagctt gtgcagcttc atttgaatca agtaatatcg atgtgattca
    22321 atatttgctt gttcaacctg gaaacaatga catcccaatg catcgtgcat aattgaggca
    22381 aaagtactgt ttaaagacta tttaaaaaga agctaagttt tatgatgctc cagaagtgaa
    22441 cttttttgtc taacttctag gattgtctct aaagcttagt ttctttttct ttatattttc
    22501 ataataaaag tttggttctt taatttttaa acatgacaga aaattttttc ttcgttatac
    22561 ttgtaataga atactatttt ataacaaagg ggatcgaatc aatgaaaaaa actgttattt
    22621 atactagttt atttgcagct ttgtttttag ctgcttgtca atcaaatacc aatcaaggca
    22681 catctgggaa tacgtcttcc agtagcgaac agcgctcttc acaggtatcg gactcctcaa
    22741 caaaagcaag cacatcttcg tctagccaga caaaaagttc tagtgaaaca agtactcagt
    22801 cagatatcaa agtgtctgca gctcgtgcga tcgaattgtt ccaagataaa tacccagata
    22861 ctgcaattac tagtttggaa ttagatagtg attggggatc ttacttctac aagatagaag
    22921 gtgtcgatga tcaaaaagaa tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga
    22981 cagagaatcc agaaactcta gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg
    23041 gtctggatgt tgataaattg atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag
    23101 taggaagcgg tacggcaaca gattgggatt tggataaaga tttaggtact acttattggg
    23161 aagtcaaagt aaaagacggc aatcaaagta catcagtcaa gatcaatagc caaacaggcg
    23221 aagtactgtc aactgaacaa gatgattaag aatttcttcg gaaaacaaga gttgattttt
    23281 cttttagtca tgatacacta gacaaaataa cttgtttgaa ggagcgattt acttgagttt
    23341 agaaacgacc tttgccaatc atatctttgc caatcctttg atgaatgcat ctggtgtcca
    23401 ctgcatgact acccaggaat tagatgaact ggctcattca gaagcaggag catttatcac
    23461 taagagctgt acgatcaatg aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc
    23521 tttagggagt atcaattcta tgggacttcc aaacttaggt ttttcttatt atctagagta
    23581 tgctcttgcg tatgaaaaag tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc
    23641 cgggatgagt gtccaggaaa acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa
    23701 tgggatcact gaattaaatt tatcttgtcc caatgtgcct ggaaaaccac aactagctta
    23761 cgattttgag gccacttatg aaacactgaa agaagtattc agcatctttt ctaagccgct
    23821 aggaatcaaa ttgcctcctt attttgattt tgctcatttt gatcagatgg ctgatatctt
    23881 gaatcagttt ccattgacct atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat
    23941 cgatacagaa caagaagcag tagtcatcaa accaaaagaa gggttcggcg gcattggcgg
    24001 agagtatatc aagccgactg ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc
    24061 agaaatccaa atcattggta caggtgggat tcggacagga caagatgcat ttgaacatct
    24121 gctatgtggc gcaagcatgt tacagattgg taccgagctt cataaagaag ggccggaaat
    24181 tttttctaga atcataaaag aattaacgca gattatgtca gaaaaaggat atacgtcaat
    24241 tgacgaattt aaaggcaaat tacgcaccat ttcatagcaa agagacgaag gtcatgattg
    24301 atcttcgtct ttttttataa aaaacactcg tctgttcatt cctgtgcaag cgtactcatt
    24361 gtatgttaga cgaacttgtg ggaaaataac agaggcaaaa aggaggataa tatgaatgat
    24421 gcaacagaat tagcaaaaga gcttaaagaa aaagaattta gttttcaaga actagttacg
    24481 gatatacaga aaaaaataaa gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa
    24541 ccattcgaga gtaattccca cgagctattg attccccagt cagaaaaagg gccattggct
    24601 ggtatcccat ttcctttaaa aatgctcggt caagaaaaaa agggatggct agctacttca
    24661 ggatctcgtt tatttgaaac acaccgagct tctcatacat ctaattatgt gcggcaagca
    24721 gaagcgatag gtctagttcc ttttggacaa accaatgcgc cagagtttgg tttcaaaaat
    24781 attacagacc cagtcatcta tggcccagca agaaaccctt ggaaccttga atgtacaccg
    24841 ggcggatcaa gtggaggagc tgctgctgca gtttcatcag gaatcgttcc tttagctggt
    24901 gctagtgatg gcggtggttc tattagaata cccgcttcgt tttgcggatt gattgggctg
    24961 aaaccttcgc gagggacgat gccagttggt ccatatgctt ggagaggatg gcaaggagct
    25021 gctattgatt tcggcttgac agtttctatg agagacacgg aggctttatt ttatgggatg
    25081 agaagtatcc attccggtgc gccatatcaa gtatcgccag ttgagtggca aacacatcca
    25141 agaaaaaaac ggttgaagat agctttttgt gttgattcac cgatccactc acagattagt
    25201 gaagaggcag tacttgctgt aaaaaaagct gtagatttcc ttggaaaaga agggcatgat
    25261 ttgacagaga ttccttaccc actagatggt cgtaaactga tcacgtctta ttatcagatg
    25321 aatggcgcag aaacagcagc aatgatccgc tcgattgaaa caggcatcca gcgagctgtc
    25381 agaaaagaag aacttgaacc catttcttgg actttgttcc aatatggtca gaagctctct
    25441 gccgctactt acatcgattc tctgcatgta tgggatgaag cagcaattgt catggaaaac
    25501 ctacatcaag aatacgatct attcctttca ccaacgactg catttaccgc ccctaaaatc
    25561 agcgaagatt tgcaaagtga tgcgataaga gcacagatgg aacacatcag tgattgttct
    25621 gaagtagaag gtttagacgt tgtttcttcc atgtttgagg agagcttgaa gatcacacct
    25681 tatacacagt tggctaatct gactggacaa ccagcaatca gcttgcctac tcatttgaca
    25741 aaagaaaaca tgcctctagg aatccaattt atggctgcca gaggaagaga agatttgcta
    25801 tttcaaatcg gctatctatt tgaagcaaat catcagttcc atttaccaaa tccatggaaa
    25861 tagcagaaac aaggagaaga agaatgaaga aaaaaagatt gtttgctcta ataactagtg
    25921 ccttttttat tttaagcgct tgcggtacag ctgccgctaa tcaggaagat aacagctgga
    25981 gtaatgtaga aaaaaagaaa gaattagtag tagcgacttc tggcacactg tttcccgcat
    26041 cttattataa tgatcaaaat gaattagttg gttatgatgt agatttggca aaagaggtgg
    26101 ctaaacggtt aaatgtcaaa atctccttta aagaatacaa cgtggatgga caaatcactt
    26161 ctgtttcaaa aagagaatca gattttgctg cgaacgattt tggtatcaca aaagaaagag
    26221 aaaaaaaatt ctccctttca gagactttca aatattcatt tgacagtatg atcgtacgaa
    26281 aaaaagatga ttcaggaatc cactcacttg aagatcttaa agggaaaaaa gcagctggcg
    26341 aacccaatac tagctatatg cgtttagcgg aaaaacacgg agctaaatta gtcacatatg
    26401 acaatgccac gaatgatcag tatttaagcg atgtagcaaa tggtcgaaca gatgttattc
    26461 taaatgatta ttatctgcag aaaatggccg tatctgcttt accagatatc cctgtaaaaa
    26521 tactggagaa cgtttatttc aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg
    26581 atgctctgaa aaagaaaatc gatggcgtat taaaagagat gaaagaagat gggacgacaa
    26641 agaaaatatc tgaaaaatat tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg
    26701 ctgagtcggt gagtgagtga tgtactttcc tctaattgat ttttccctca tgtggacttc
    26761 acttcctttc gtactgcaag gcttggtata cacattaggg attggcttcg tgtcgtttct
    26821 tttaggaaat ctggtagggt tgcttttgac agttttaggt cttttggatt ggctgcctct
    26881 gaatgtattt attcgttttt atttatcgtt ctttagaggc attcccgcac tagtcctgct
    26941 gtttctgtta tattttgggt taccttatca attaagtgcc ttaacggctt cagtcatctg
    27001 ctttacgatt accagcagtg catttattgg tgaaatctat cgaggatcac tagcaggcgt
    27061 ttcttcaggt cagtgggacg ctggtttagc ttctggtttt tctttctttg gggtattgaa
    27121 atatatcgtt ttgccgcaag cttttcgaat ctcagtaccg gcactcagta acgtagctat
    27181 ggatctagtg aaaggtactt cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa
    27241 agcaaagatc ataggcggtc ggacattcga ttatttttct ctctatattt tagttgcact
    27301 gatttattgg gtcatttgta tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc
    27361 tatgcatcag tcaatctgaa aaatagaaat ggtgctagcg tggctggcac catttctatt
    27421 ttttgtttct tgtatattct gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg
    27481 aaaatatgtt cggaggtaga aaacatgaag accccgctaa ccatcgaaat agaatcctcg
    27541 ttataccaat attgcatcga gcaaggcggc ttggttgtag aagaagtcac gatgcctgat
    27601 gaccaaggaa ttgtagatac gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg
    27661 cgatgctatg agctgaaagt gacaaaagca gattttcgct ctacagccaa actgtcattt
    27721 gttggacatt ataattattt tgttttacca agtaaactat atgaagaagt aaaaactgaa
    27781 atccctacag gaataggtgt tttagtgtat cgtccgtaca cggttgaaga ggagatgcct
    27841 gcctctggta catttctgat tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa
    27901 gaagctttga caaaccgctt catggcttct ttattcaggg aagtaagaaa agcaaagcaa
    27961 atggagcgag gcgtaaaata ttttccaaca gatcaattgt acaaagaatt gaaaagacgt
    28021 tcagaaaatc aagatccatt ttctaacgaa cgatattatg aacggttttt agaggaaata
    28081 gctagtcagg caatcaatga gttgcaagaa gaagttatcg cgcttcagca tgattatgaa
    28141 tatttgaggc aacaacagca gatccgtcgg ttgcctacag agccattgga gtgaaaaata
    28201 tgtactatcc ttatttaaga ggaaaacaat ttgacctatt agcattaaaa gaagcactgt
    28261 cgcgaggatt gctctccaat aaaatccaac cagtgatcga accagtacgt gattccgcta
    28321 cgcttaaaaa tgtgatcgaa ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa
    28381 atccccaagt aggacaattc aaattattcg atcagcatgt gcacagttgg acagtcaaag
    28441 aaaatagctc tgtcgtccct gcacagatct taacgccaga aaatctcagt gaagtactgg
    28501 attcacctcc agcgtttctt gttttcgatg gacagcacta cccaaaagat acagaggtgt
    28561 ggaaacagtt ggcaggagtt gattcgaaat ttttgatacc agatactagt cgctttcgca
    28621 tctggctgcc cgaaaacaaa attgtgatcc gtgactcttt tcaaacgaga aagcatgttg
    28681 aaagttatgc agataaaaca gatgactttt ttagtgatga ctatcttttt ttccacgcag
    28741 acggatatat tggattttct gatttcacga tcgaaggtag ccgttatttc gataaagggt
    28801 tccctagccg agcgcttgct atccatctaa cgtatattga tgcttacggc aatatacgcg
    28861 tgaaacattt tgtctctgac agtaacgact ctgctaaaga ccaagcattg aagtttcttg
    28921 aagcaggaga taaaatgaaa aaatggatca tgcgtcacca tcatcaactc ttgattactt
    28981 caggaatgat tgaattattg gctctttacc agcaacaaaa attccctgga ctgggagtgt
    29041 tgaaaaagtg gtcacttatg catcatttgg aacttattag tcacctgcta gatcatccaa
    29101 aagattggct taaatattat tcaaaagttc aggaactata tgaagtgatc caatgattag
    29161 ataatgtaaa tgaaaaagga atagaaaaga ggaaactgga tgtatattcg gcaaacaagg
    29221 aaagaagaag tagaagaagt ggtagaaatc attaatgccg cagcggattt cattgcttcg
    29281 caaggctctc ctcaatggca aaacgggcag aaaccaacaa aagaaacgat tttgaacgat
    29341 attgaaagaa aagaaagcta tgtattaata gtggaagatc aaattgctgg aacagctgca
    29401 ttagtctcaa gcgtggatcc cgtttacaca aaaatagatg gtagttggaa agaatcaact
    29461 ggtccttatt tatcgattca tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta
    29521 gcaaaaccgt tcttagaaca tttgttgacg attgcagcca gcaaaaatat ccaagatatc
    29581 cgtatcgata cttatccagc taatgaaccg atggaaaaga ccatctattc tgctggattc
    29641 acttatcaag ggatgattga atttcctttt gaacacggcg aaagaaaagc atatcaaata
    29701 atcatcaatt gactataaaa tcatctgagc aaagataaag acattaacac tactgaaaaa
    29761 aacaatcata tgatagccaa gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa
    29821 gaatttccga aaatgcttct caaatttttg tggaatttta gcgtatttta cgaaatagct
    29881 gctttctgaa actcccttca tcgagaggtc gtgacatagc ttttgtcacg acctctcggc
    29941 ttatcctacg agtattattc gatacattta tatcaataat ccttttttta ttttatctgc
    30001 agcttcttgg aacgctccat cgtttgacac gactaaaagt gaggggatag ctttcaaacg
    30061 tttcaactgt gctaaatctt ctacataaac cgccagtctg gctgaaatct gttcagatga
    30121 atctccacgt tttttcaggc gcgattgcaa tgtttcttta gagatcgtaa taaagatagg
    30181 acggactacc gtcccgaaat actcatataa atgccaaaaa ccatttgctg tcaaaacggc
    30241 gtaacaattt cctactgtca gtttcttttt aatttctgct ttactggaac catatttatg
    30301 ttgagcatat tggtcccact ctgcaaactc atttcccttg atcatctgat tgaatgtatt
    30361 gtcagaaaca aaataataat cctcttgatc tttttcccca ggtcttggtg atcgattcgt
    30421 gtaagtaatg atcttttgtt ccggctgaaa aatagcttct gcgagtgaag tttttccaga
    30481 accagaagga ccgataaata catagcaata attcattgtc atttctcctt tgatgattga
    30541 acaagtgaaa aagctttgga aaatatttcc aaagctttta aaagtaactt tgcttttttg
    30601 tatattatac cagcaagcca gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta
    30661 gcaaaatttt caatccgtgt tttgcgacaa gatttccttt ttcttcattt aatcccttca
    30721 tcgcaccaga aatgatcccg atagaagaga agttggcaaa tgaaacgagg aagacagata
    30781 taattgctgt tgtacgctca gtaaaatgga aattaccatt tgctaattct gtcatagcaa
    30841 caaattcatt tgtaaccagt ttagtcgcca tgatactacc ggcatccact gcttctttcc
    30901 atgggattcc gctgatgaag gcaagagggg caaagatgta acctaggatc tcttggaaag
    30961 tgatgccgaa tatcccgtgg aagatagcat tgatcatggc aatcaaagca acaaaaccga
    31021 taagcatagc agcaaccgtg atcgccacat ggaaaccatc aagaatgtat tctcccaaca
    31081 tttggaaaaa tgtctgttct ttgttttctt caatgatcag ctcatcttct ttttcttcta
    31141 atacataggg atttacaatt gaagccagaa tataaccgcc aaataagttc aaaacaaggg
    31201 cagtgatcac atatttagaa tcgatcagtg ccatataaga tccgacgatc gacatagaaa
    31261 ctgtagacat agccgataca ctcatggtga acaaacgttt ctcagatagg aagggtaatt
    31321 ccttttttat ggaaataaaa acttccgatt gtcctaaaat agcagaagct actccattat
    31381 aagattccaa tttgcccatc ccgttgattt tacttaatcc tagaccaaca gctttcataa
    31441 ataatggaag aatcttgata tagcgtagaa taccgataat agcagaaata aatacaattg
    31501 gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa
    31561 agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa catttttgcc aatacagaaa
    31621 caaccgctgt accaaaagtt gtacgtaata atacgaaacc aagaacaagc tgtaaaacga
    31681 acatcacaat tagtggtttc aatttgattg cttttttgtt actgctgatc aaaaaagcaa
    31741 gccctaatgt gacaaataaa ccaataatac ctaataaata actcaaataa tcactcctat
    31801 tccaaattcc gttttatttt acctgttttg caaataaaat actaatcttt agtgaaattt
    31861 tcattacttt tttagagtga aacgcattca ttttccatta gaaaataaag cgaacaaaat
    31921 aaaaggttta aaaagtgtaa cgtcttagta aagtctataa aaaaacgaaa ctatcgatta
    31981 agctaatagt ttcgttttca acatttataa gtaaactttt tctattggta atatagattt
    32041 tcggaagggt agacaggtgc gcaggtaata tcgattccta gttttttcaa tgtttgttca
    32101 tcatttttat tgaccatcac agtagagtgg gcttgtgctc cctcaagttc agctagttta
    32161 tcataagcaa cttgagcgat tggattcgtt actgcactga ttgataaagc gatcaagact
    32221 tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt
    32281 gtttcaagaa ctaatggtga caataaagga atgtcatctg cgatatgggc taaatgtttt
    32341 actgcattta agataactgc cgctgacgca tccatcaatg aacttgtacg cccggtaatg
    32401 atgacttcat ctggtaattc gattgcaatc acagcagaag gatcttttgt aggactttgc
    32461 gcttgaatct tatctgcata gtcatgggca gggactacca cgtttcggtc ttcaggacgt
    32521 aaaccgactt cttccataat cagtttgatt cgattaactg cttcttcgtc gatcaatcct
    32581 tttttgaagt cgcattctgt ttggaaataa cgacggatga tttcttgttt agaggcttct
    32641 tggacaacgg tatcatccac gatcccaaat cctacacgat tcacacccat gtcagtagga
    32701 gattgataaa cagattcttt accagtaatt ttttcaataa tacgtttgat tacaggaaat
    32761 gtctcaacat cccgattata gttcacagct actttattgt aggcatcaaa gtggaaggaa
    32821 tcgatcatat tgacatcttt tagatcgact gtggctgctt cataagcaat gttcagcgga
    32881 tgtttcaacg gaacattcca tactgggaaa gtttcgaact tggagtaacc agctgcatgt
    32941 cctttttggc tttcatgata cagttggttc aaggaagtag cgagctttcc acttcctggg
    33001 ccaggagctg tcactacaac gattggttta gttgtttcaa tatactcatt ttttccaaaa
    33061 ccatcttctg agacgatttt ttctacgttg actggatagt cttcgatttc tgagtgttta
    33121 tagaccttga tgtttcgtct ttctagttta ttgataaaga ctttcgtggc aggctgtcca
    33181 ctatagcgag tgataacgac actgttgatt aataagccat aaccacgtaa ttcatcgatt
    33241 aaccgcaaga tatccatatc ataagtaatc ccataatcac cacggatctt attacgttca
    33301 atatctccag catagacgca aatcagtatt tcagcttgat ctttcaattt ttgcagcaat
    33361 ttgattttcg catcttcttc aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact
    33421 agcttcccac caaattctaa atataatttg tcatagtggt tcactcgttc caaaatatac
    33481 gctgactgct cttcaatata tttttctgaa tcaaaaccaa tttttttcac taggttccct
    33541 ccttaagtac taaagcattt tttcattttt ttgaggattc gtcaactgga agagcgaaat
    33601 tcctcaaaaa aacaaagaaa atggtttaaa ccaacttgtc tagatagtta ttcattggta
    33661 tgatagtttt tgataaggaa ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata
    33721 taaacactat atccgttcga tttttaccgg ttatgaaaga atgcttgaag atcccgaatt
    33781 ggaacaattg acgtttactt atctaaacga agaaacgcag ctaactcgtg atgagcatca
    33841 gcgcattcat ttcacaacaa gagatctgcc tagtaaataa aaaataagat aaaggtcatc
    33901 ggattcgatt ctgtcctatc agatggaaag actagttgat cgaaaaaaag gctgtaccaa
    33961 attttggtgc agcctgtttt atggagcatt gcccgctctt ttttataaca attttttaca
    34021 gaaaatggta ctattgaagt attaataagt gaaaaacaga tgagtaagag gaggcaagta
    34081 gtatgaaaga gccagataca aatgcaaatt atgagccaga cgaactagag gagacgaatg
    34141 cggatcagga tacaggacaa gaatcgtctt ctttttacga tatcaaagaa aaagcaagca
    34201 tgaatctttc ccaattatta gaagacttac aggcttttga aaaagctgtc cgcgaagaag
    34261 atatccctga gatctaccgt ttatacaatg gccgattgaa tgaagaatta aaaagaagct
    34321 caaatgaaaa tcacgagatc gatcaattgc tgatgcgtaa gatccatgat cgttttttac
    34381 aggaatttcc ttttatggaa caagtagaaa acatctcgcc aacgatgagc tattacaaaa
    34441 tcggcactta ttaccatgat cggcccacgg tcggtattga tgcaagtctg ccagaaattt
    34501 ttgttttgcc taagatcgat gaagagtggg agaaatattc ccaaccggag tcaacggact
    34561 atgacaaaaa aatcaatgag ctagacgcaa aggtgatcac tgctcagaca gaaattgaac
    34621 gtttagatga acaaataaaa gaaatcaatc gacaaatgac tgatttgaac gacaataaag
    34681 gattcttgaa tcgtaaaaaa atcgaggaag aaattcaaga attagagaag aagaagcaag
    34741 tactatctaa tgaaaaatta ggctggcttc cttatattga gacgcctgaa acaatccagc
    34801 agcaaaaaga agcattgaag caagaagcaa gagcagacca gcttcgtgca gccatcgtag
    34861 aaaaagaaca aaggcaaatc agacgctatt tcggtagcaa agagggattc ggtcaagcaa
    34921 tccatgaatt cttgatgaat tatcttggca atgagaatcc taataatcaa tctaatgaag
    34981 gaggttccga gtatgaatga acacgatgaa gcagaatgga caaaaaatga agaacaaaca
    35041 gaagatcatg aagaaacgaa cacttcattg gaagaacaaa caagtaatcc tctggatgac
    35101 acagaagaaa tccctatgct agaggaaaca aaaatacacg aaaaagaaaa agtaactccg
    35161 acgaatacga atactgcaga aaacacccat gcttacgaaa taactgaaaa tcccgggtat
    35221 ttagaaggaa aagagcaaga tcatcaagat atcgtcagtt tgattgaaac agcggaagca
    35281 gaattagcgg atttacgtat gagaaaagat ttgatcgaat cagattttcc acaattatta
    35341 gaatcatatg atcgttttat attgaatgaa gaaccaatct ccacgatcgg aaagacaagc
    35401 ctgatcgaat acttcacgta tgaattactg aaaccgttga atgatattgg tctggaacgg
    35461 tcaaattcag cttatgacac atggaaaaca agacattttt cgatcaatta tacgttgaat
    35521 ggacaaaacg aattagtctt ttcctttgta ttaccgacca tcgatcaacg ataccaagtg
    35581 gaatcgatga gtctcctaaa cgtatcacca gagacaatgg aaatcaatgt ccaagatgac
    35641 cgggtgcttt ctttgatccg acactggtca gtggatcgag tattctctgc agggcagatc
    35701 acgatattca atcacaaatt gaatcaatta ttagcccatg caagaacact tggttttacc
    35761 gttaatcaga cattactaga caatacgaag ccacttcgtt tgaatcttca gagcgaattt
    35821 gaattgacga atgaagtatt ggatgatatt tttatcgtgg caatgaaaaa tccatcctac
    35881 gatatggaaa agatcaacga aactacttat caagtacgat tagataaagg acagtcttta
    35941 acgataacaa caaacgaaaa agatcaaaca gtcttgtcga tctcatctgg agactaccca
    36001 cgatctgtca ttgatttctt tatcaattac gaattcttgg tcccattaat ggtgagaaag
    36061 tcctgattgg ataagttgga ttaccagatg tcattcagat aattctgttc caaaggactt
    36121 gcggcagtta tccagaataa ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa
    36181 taacttgctg atgaaggagg gatttcatta ggaatgtcca caaataaaat agcacagctt
    36241 agcttattgt ctgctgcttg tgtagcaggt aggatcgctt ttcaatttat tccgaatttc
    36301 cagccaatga ctgctatttt tttatttata gtactttact taagcttaaa agatgcgtta
    36361 attgtcatga gtctttctat tgcgatcagc agtttttatt ttggtgttgg accatgggtc
    36421 atcggccaat ggatcagcta tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga
    36481 aaaacagtgc aaaggaatct atggttaaag ggagtctgtt tttttctggc gggcatggtt
    36541 tacggaatag gtatgaccgt ttttgatacg ctgttatatc gtttgcctca accttggatc
    36601 tattaccttc aaggggtgtc gtttgatttg atgcacagta taggcaatgt tgtgttcttt
    36661 cttgtttttc tgccagtcgt caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa
    36721 aataatttat agtttgcttg caatgagtgt cagtatactc attttgacaa gttgcagtac
    36781 aacctataca caatcggctt ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac
    36841 aatcacgtta caagaaaatg gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg
    36901 cgatgattta ctgagtattt tgaaacgaaa ttttgaagtc aaagaagata acggcttcat
    36961 cacttcgctt gaagggcact cacaggatga gaaaaatcaa ctctattgga tgtttactgt
    37021 ggacgggaaa agtgctacta ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt
    37081 cattttcaat ttgtctaaac tataagtttc ataaaaaact gttaaagggc gaatattgcc
    37141 ataattcagt gagttcagtt gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag
    37201 cggaatgttt tccgtaattg ctgctctagg ggctaatatt ctagttgcta tcagcaaatt
    37261 tgtcggctat gcaatcagtg gcagtgccgc catgctaaac gaaagcatcc acagtatcgt
    37321 tgattgcagc aatcagattt tgctgttatt cggtgataaa caagccaata aaggacagag
    37381 cgagttgcat caatttggtg aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat
    37441 gatgcttttc ttcggcggtg gggcacttgg ggtgatggaa gcttttgaaa agttgacgca
    37501 tccatcccat gaagtgggta atccgatcat tgttatagca atactgttat ttggtttggt
    37561 aatcgaagga agttcgttac gcgtggcaat gaaagaaatc aaagaattga atactgaaaa
    37621 actttccacg atgcgctttt tgcgcgaaag tcgacacagt gaaatactga ttatttttac
    37681 ggaagacttg tgtgctgtaa tcggtttggt cttggcttta gcaggaacaa tattgactta
    37741 tgcaaccggg attgctgcat ttgatgcgat cagcggattg ctgatcggtt tcttgctgat
    37801 gggagccgcc attttcctag caaaagaatt ttatagtctg atcatcggtg aaagcgtgac
    37861 agcaagcgat ttatcaaaaa tcaagttagc ttttgaacgt cctgagatca gtcggctgat
    37921 cgatataaaa acagttcatt taagtccaac ggagatttta gttgcagcaa aagcagatat
    37981 cgttggtaac aaagagaatg aagcttactc ggtcatcaat gatattgaaa aaaatatgcg
    38041 tcagtctctt cctgacaaga agatgtatat atacattgaa acagataaat atgacccaaa
    38101 ttattctcgt aaataaaacg tttattcatg aattgaagca aaaatgagaa aaatataatc
    38161 ttgtttttaa ataaaacggc tacaatttag cgaatttatg ttacaatgag tccgctgttt
    38221 atttgttgta attaaaggag atattttaat gaacaaaaag aaaagtgcgc catggcttca
    38281 agctttgcat gcagtgatgc cactttgtat aagctatata ccagtaggat tagcttgcgg
    38341 agtattgttg caaaaagttg gatttgatcc tctcttatca ggactgttat cgattctggt
    38401 cttttccggt ggcgcgcagt ttttagttgc atctatgctt acgacgcaag catcatttgc
    38461 aacgacatta ttgatggttt tctttttaga attacgctat acattattag gagcaagtct
    38521 tgccggtttt atgaaaaaag aaaagcggac gtttttggct gtcttttctc aatcactgaa
    38581 tgatgaaaac tatgcggtga actacttaaa gttttcaact gacccttcct ggaataaaag
    38641 aaaggcttta tatgtcaatt ggttctcaat gactgcatgg gccggtagca atatgatcgg
    38701 taattttttc ggctcggtga ttcgagtaga tgctgacctt gtccattttg ctttgacggc
    38761 catgttcatt ttcatgttta tcatgcagat gaaaagcgca ctactgatct ttacaggttt
    38821 attctcaggc gtattagggg tcgtattcat ggtattattc caaaatactt tcggattgat
    38881 tgcagcaaca gtcattgctt catttgccgg atttatgctg gagaaggcat tcaaaaaaga
    38941 aaaagctaag aaactaagag accgcggtaa agatgtcaat gagccgctct tcaacttccc
    39001 ggaacaggag gccaatcacc atgactaaac tatatttgct acttttagtt gcttgtctgt
    39061 tcatcgtggc atatatcccg cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc
    39121 catcctggtt cagtgactgg atgaaatatg taccggtggc attatttgcg gcgttagctt
    39181 ttaaagatgt tttcattact catgaacatc tggatattgc ttggaatatc aaaatagctg
    39241 caatgattct agtagcaggc gtggcttaca aaacacgttc gatggcgttg tcagttttga
    39301 caggacttgc gtctgtcttt ttgctgtcta tgctttaaac tgttttattt tattaaaaac
    39361 agagattgcg acataagttg ttcaaactga tctatccgaa ctgcatttgc aacagtcaat
    39421 tctggatgca agtttattcg tagggataaa aaagattagt gttggcttat gatgcaagct
    39481 cttttttatt ttaggaatta gagagggatt tgtccttcta tgattttttg ctataatagg
    39541 gaagatgttt attttatgaa agaatagttg agggaattta aatgaataaa aaattgatgc
    39601 aaggaacatt ttggctaacc tttgctaatc ttctatgtaa agttttaggg gtagtctatt
    39661 tgattccctg gctaagtatg atggggaaca atcaagatgg tatgcttgct acgacattgt
    39721 ataatgttgg ctatcttcct tatggattgt tcctgatgct tggtacagtc ggatttccaa
    39781 atgcgattgc aaaaaaagta gctgtagcca caaaagaagg agataacgct gcttgtcggc
    39841 tgatttttag aagtacgatc aatatcatgt ttgtcatcgg tatcgcctct gctgcactga
    39901 tgtatttatt tgctccttta ctagccggaa tcagtccgat tgctaacgtt aataatggga
    39961 tactagctat cagaagttta tgcccatctt tgattgctat cccaattctt agtgcaatga
    40021 gaggatattt ccaaggaaaa aatgatctgc ggccttatgg aacttctttg attatcgaac
    40081 aggctgtgcg agtgatcgtt atcttggctg gtacttatta cttgcgcgta ttgactgacg
    40141 gaaccatatt agaagctgta ttgatcagta cagtcgcttc tttcttcggt gggttagctg
    40201 cgattatcca tatgtatatc gtcggacgca gaaaagactt cttcgaactt aaagattttt
    40261 tgatttccag ccgttatttc gaaagagaaa accgatctgc ctctgtctct atcattcggg
    40321 aaacactacc gtttattttt gtcggctcag tcattactat cgttcaaatg atcgaccaag
    40381 taacgatgaa accactgctt cactttttcc gtccagagat cgcagatcag caactggaat
    40441 tcttattcag ccgtgcttct gtcaatccaa ataaattgac attgatcctg atctccatgg
    40501 taggaacagt agctataagc agcttgccaa tattaagcac gatgaagaaa aaagatcgga
    40561 tacaaatcga aaaaacagta ggagatagtt tttcgattgc gttattgatt cttcttcctt
    40621 cattgactgg aatgtcttta ttagctggac cactatacac attgtttttt ggctatgatc
    40681 ctgaaagtgt cggatatttc caaatggctt tgctagcttc tttgtttttc tctttattca
    40741 cgatcctatc tacgatgctt caatctttga atcaccatcg ttttgcgatc cgattaactg
    40801 tagaagcaat tatcctgaaa gtcatcttcc aagtaatcgg attaggatta gttggcggat
    40861 acgggatgag cttatcaaat ggcttagcat tcggtattgt ctttgttcgc ggctatcact
    40921 ttatgtgcaa agaataccga atagcacctt tagcaaagat cagcaatttt ttcttaaaaa
    40981 ctttccgctc caccctgatt atgcttgcag tatgttttgc cgttttcttt ttgctgaatc
    41041 agcgattgtc aatggaatca aaaagctatg caatgattta ttgtgtggct gtaggaagta
    41101 ttggcggctt agtattttta ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa
    41161 atgttaaaca tcatagcaga agcaaagaat aggttaacta atcgtttttt gctgtcactt
    41221 gaaaaacggc aactcgtatg attacgcttg tcgtttttct attttccaat tttagtttca
    41281 tatctaatct tacttatgat atagttataa ggtctggaaa aattctttga aacgaggaaa
    41341 ttaaacacat gaaacttgca gtagttacag atagttcggc atatctgccg gaacgtatca
    41401 agaaccatcc cgacttattc gttattccaa tccctttgat tatggatggg aaaatttata
    41461 acgaaggaat cgatatcgaa gcggatgaat attatggact tttaaaaaac agtaaagatt
    41521 ttcctactac ttcacaacct gtcgtagggg aagttttggg tttatatgag gaattgaaag
    41581 agaaagggta tgacacgatt ttaagtatcc atctttcttc agggatttct ggctttgtca
    41641 acacattatt tgcgatgaaa gatgacatca gcggagtaac ggtcattcct tatgactcta
    41701 agatcactag tatgccgatg ggacacatgg tagaagctgc cttagactta aatgatgaag
    41761 gtcagtccct tgatgaaata cttgcacata tcgatcgaat ccgagataat acctacgctt
    41821 atctgattgt agatgatttg aacaatttag tacgaggagg acgtttgaca aatggtgcag
    41881 cgctgattgg cagcttatta aaaatcaaac cgatcctgac attttctgaa gggaaaattg
    41941 ttttattcga aaaaatccgt tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg
    42001 gtagaagaaa taacgaaata caaccgccag tcaaacttta cgtgatacat gcaaataatt
    42061 tagaagtagc gattgcggag aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg
    42121 aaattggtca tttcggacca gtcattggta cacatttagg tgaaaaagcc atcggcattg
    42181 ccatttcagc gcaataattc cttttattca aaaaacatac caaaaatatt ccaacatcat
    42241 cttggacttt ctacatgtta ctaatgagcg tgtaaaagaa cgagatggtg tttttatttc
    42301 tatcaatgaa gctctttttc tttgcaatcc tcacttcaat cctctataat gtcgcatata
    42361 gtttagtaaa tgagtaaact actaatttac ttatttacta aaaataaaag gatgaggagt
    42421 taaaataatg gaaataccaa caaatttaaa acataaacca gtaatcgttg tagaagacta
    42481 tgataaagtt gatggaagaa atgcattaaa tacggatgca aaagggcttt cactgggcct
    42541 tgctcagtgg aatgatcgtg gaaaagtcga tatctcaggc aaggtatggc gacatactgg
    42601 agaaaaatgg tcacgacagt cggaagaatt accaatcaca cgtattttag atttagcgat
    42661 cttagctgca caaggaagtt tatacttcca agatgcttat cgttatgaaa aattttatga
    42721 tccagaaaat ccagtcgtag acatcattgg attacaaggg aaccgaatga cggtagaagt
    42781 agataccaaa aatccaaaga tcgatgaaga catcattttg tattacgata cactacaaaa
    42841 agacggagaa ctgattgggg aacgattccg tattttaaaa cgtcttttag atgagctagg
    42901 atattaatag acagagatga tcaaaagggg aagagaagca tggagaattt ctcactaaca
    42961 gaaaaccgaa ccttttcacg gctagaaaag caaatgatct ggaagaaacc gaaaagccat
    43021 cggacaagtg cagaagaatg gcggatcgcc tccgaaatca aagggaattg gcaagatcct
    43081 gaaatgaaaa tcagtaacgt acttttggaa ggagatgccg ggtcggggaa gacacaatta
    43141 gccaaagcac tatctgctga tctgcaactt ccctatacaa aagtgacctg ttttgcagat
    43201 atggacaaat cagatgtttt cggagcctta ttacctgtag tagattctaa taaggaacaa
    43261 gaccaagaat tactagaagc tatttaccaa acagatacac ttgaagctgt actgacactg
    43321 atccagaacc attacggcat tgatcctttt tctgctaaag aaaaattagc agagctagtt
    43381 caaaggatcg aagctgatta tccagatgca atccattaca gctattaccc ttctgaaatc
    43441 gttcgagcga ttgaaaaagg atatttatta gagattcaag agccgactgt catccgagat
    43501 gcctctgttc ttgtggcact taactcagcg ctcgaaccaa atgggatgtt gaatttaccg
    43561 acaggaatca ttcgtcgcca tccagattgc gtcattgtga tcactacaaa tcgaaattac
    43621 caaggaaatc gcccattaaa tgaatcttta agagatcgga tgcaacatgc ggaaaaaatg
    43681 gatcttcctg ccttagaagt catggcagaa cgtgcgatgg cgaaaactat gatccaaaaa
    43741 caagaacttc tcttaaagat ggcagagatt atccgtttgt tggatgaaac tgcaaaagct
    43801 aacgccatca aaggtgtagc aggaatgcgt tcttattttt attgggcaaa tacctataag
    43861 caaggacaag atttactcca gtcgatttat ccaaaagttt tgtataaatt atccactgat
    43921 tcagatgaat tggcaattct tgaacaagct ttgtctgaca gtggattgct actggaactt
    43981 caggacatat tgagaaaaga aaaatggggt catcctcaat ctgagcatac aaaaggaaga
    44041 accattagcg ccgaagaagc aaatgaacga aacatcactg aaaatgctga agaaattctg
    44101 cgcttagatg aaaagaaatc gttgtctgct gaagaaccaa atgagggaga agagaaacaa
    44161 gaagaaacaa tagaaaaaaa cgagattcct cctcaaatgc aaaaagaacg cagtagcgat
    44221 atcgaaggaa aaaatcaaaa agaacaacaa acaggagatg attctcaaga aatgtctgct
    44281 tctgacatgg aagcacatga taaggaaatc aagaagcaac tgaataaaga agcgcgacaa
    44341 atcatgaagg aaacgattca tgaaaaagaa ggtctcatcg ttcaccgacc agctttccaa
    44401 ggaaaaacgc cggaagcacg tgagctacac atgcaagtaa tgccaatagt agaatcactt
    44461 agccggcaaa ttttagagtt actggataat gaacaaacag agacgtacca aaaaggaaaa
    44521 tacgaaggtc agcggttcaa tgcaagcaaa gtagcatatg gtgatttgag aaattttgac
    44581 aaaaagaacc cgccacatga gcaaccttct ttagcggtag ctgtacggat cgatgaatct
    44641 gggtcaatga tacgggatga tcggatcgaa gcggcaaaaa aagcagcgat tgccattgcc
    44701 gaattttcta agaaagtcga tatcccattg ttgatttacg gagatacagc agaccgttcg
    44761 tcaagagaaa agacttcttt gttttcttat aaagaatttg aagacggatt tcaatggtta
    44821 aatgaaaaat tagtcactat gaaaccaagg caaaacaatc gtgatggtgc tgcacttcgt
    44881 ttagcagcag aaaaattaaa cagacaatcc gcaacaacca aactgttgct taatattagt
    44941 gacggtcagc caaaagcgtt gccggattat acaggcaaaa aagcaaaaga agatatccag
    45001 tctgttttga aagaatatga acgtcaagga atcctatttg tctccgcagc tattggccaa
    45061 gacaaagaag aaatcaagtc gatctacggt gaaagtcgtt ttgtagatat tactgacttg
    45121 aatgaatttc ccaagcaaat gattcaactg attgcccgct atctatgata aaataaagct
    45181 caacgaacgc acttagcaga ttcgttgagc ttttcgtttc tagttaccgg taacaaggat
    45241 acttctaaaa tagaatcggt tataatcaag agggaccgtt aaaggagtga aacgatatga
    45301 tacaagaaca aaaatggtgg aaagaagcag taggctatca aatctatcct gctagtttca
    45361 aagatagcaa tggcgatggt caaggagaca tcaatgggat ccgtgaaaaa cttaattatt
    45421 tgaaagatct tggtatcggc tttatctgga tcaatccgat ctatcaatct ccatttgtcg
    45481 ataatggtta tgacgtaagt gactatcaag atatagaaga gagattcgga acgatggaag
    45541 attttgattt attgctaaaa gaagcacatg aattagggat caaagtgatt atggatcttg
    45601 tcatcaacca tagttcggat cagcatcaat ggtttgaaga atcgaaaaaa tctaaagaaa
    45661 gcccgtatcg cgattattac atttgggtcg acggggtaga tggcaaagaa ccgaataatt
    45721 ggacatcgat tttcggcggt tctgcttggg agtattctca tgaaactgga cagtattatc
    45781 tgcatgtttt tgcaaaagaa caaccagact taaattggga aagcgagaaa ttgaaagaag
    45841 aactattcaa tatgatccga tggtggttgg ataaagggat agatggattt cgtttagatg
    45901 cgatttctca tgtaaaaaaa gatgaatatt cagtaaaagc cacagagaac cctttctcac
    45961 cctttcaaaa tgtctcagga atcgaagaac atttgaccga tttgaaacat gtttttgaag
    46021 agtacgatat catgactgtc ggagaagcaa gtggtgtcac tgcagaagaa ggaccacaat
    46081 gggtagggaa agacggttat ttcgatatga tattcgaatt tgatcatatc catatctggc
    46141 agcaagaaaa agaagggcaa ttagatgtcc ttaaattgaa acttgcatta agcgcttggc
    46201 aaacttcatt ggatggtatc ggatggaacg ctttatatat ggaaaaccat gatgttcctc
    46261 gtgccgtttc agtgtttgga gatactcgtc ccgatttttg ggctatgtct gcaaaagcaa
    46321 tcgctatgat gtatttcttt ttgcaaggta cgccatttat ttatcaagga caggaaatcg
    46381 gaatgacgaa tatgcccttt gaatcgatcg atcaagttga tgcagtagat tcgaaaagac
    46441 tttataaaag gctgcttgcg gaaggtaaga caagagaaga agcattggat atcattcgtg
    46501 aaacaacgag agacaacagc aggacaccca tgcaatggac ttcggaacaa tatgctggtt
    46561 tttctacaca tgaaccttgg ctgatcacta atccaaatac caaaacgatc aatgtagaac
    46621 aacaagaata tgaaccagag tcggtcctgc aattctataa gaacatgatc cgcattagac
    46681 aaaccaataa aggtctgata tacggttctt ataaagaata tcttcatgaa catccacaac
    46741 tttatgtcta tgaaagatat ttagaagatg aacaatatct tattatggta aacttaacag
    46801 aatcacttgc tgactatgaa cttccaaaag aagcagatca gtcctggaca ttactcttgt
    46861 caaattcctc tagcggagaa tttgaagcta aaggcatact agcaccttat gaagctcggt
    46921 tatataaaac aaacaagtaa aacttcctcg ttaaagttca ttagttggac tttgcgagga
    46981 agtttttcag attgctctgt caattactca tttcaataat tcgctctagg accaccgata
    47041 tatttaggac gagagcgaag ggctgtcacg tgagcttgtc caagggtttt caaacgaggt
    47101 ctgacaggta cttggacatg tttgatatgc ataccgattg atgtgtcacc gatatcgata
    47161 ccggcttgtc caactacgtg ttccacttcg acaggatcat tgaactggtc aaaagcagca
    47221 acagaacaag cacctccagc atgtagtgca ggaacaacag agacaatttc gaaatttttt
    47281 gcttctgcca ctgttcgttc tactactaat gctcggttca aatgttcaca accttggaca
    47341 gctagcgcaa tttctttagg atccaacaac tcttttaacg tacggatgat ccattgacca
    47401 acttccgcac tggaattttt tccaatatgt cctccaacga cttcgcttgt gctgcatcct
    47461 aaaacaaaaa gatcaccttt tttcaagtgt gcctcagcca atatgtcatt aactatttca
    47521 gtcaattggt cttttaattc tttttctgac aacttcattt tctaaaacct tctttttcaa
    47581 ttaatattgc ttccgtgctt gaatacgttg aagacttgca gttataggaa aggcaagtcc
    47641 cattccaatc agattttgga agatgtttcc tggtatagaa gccactccag ccggccaatc
    47701 atataaaatc caaccggcga taccatagcc gatgatcata actaagcttc ctaataaaag
    47761 ccctaatccc cacatacgag tagaagattt gctgaaagaa ccagcaacat atccttgtaa
    47821 cccgtggatc actaatgaaa aaactgccca ctgcggatat cctgaaatca aatcgatcaa
    47881 tagaccactg gaagcaccaa caactaatcc acccgttgga ccataaagaa aagcagcggt
    47941 atagatcccg gcctcgcata atgtcacaaa tccattcgta gccggtacag ggatcaaaat
    48001 tgttaatgaa agggcaactg tcaacgcaat cagcatagca tagatcgtta attgcttagt
    48061 tttcatttct cattcctcct ttttgaatac cgccagacca gacacttccg gtatgatcgt
    48121 tcatcagcac gccattagaa atacaatggg caacaaattt tttactttct gctactgctt
    48181 tggtaaaatc tgcccctaac acaagattcg ctgttattgc agaagaaaag gcacaaccgg
    48241 ccccgtttac tgttgtagtg tcgatttttt tatgagaaaa tacctcataa tgatcaattg
    48301 aatagagaag atccacagct tcatctccag ccatccttgt gccgcctttg atcacaatgt
    48361 tttttgttcc tgaagcagtg agttctttag cgagatggat catttctgtt aacgaagagg
    48421 gaagagcttg accagaaagt aaagcagctt ctgccaaatt aggcgtaatc atcgtaacaa
    48481 gtggaaaaag ttcttcgatg agctgcgaaa tatacaattg attgatcaca gcttcagttt
    48541 ctttgaatgc caaaacaggg tctaaaatga ttgggatatt ttgttttgtt tttatgaagt
    48601 ctcgaacaac catgattgct tcacttgaat tcaataaccc aattttgata ccatctaatg
    48661 aagtcatttt atcaatcgaa actaactgtc gtttaagcaa ttccaaagat atagattcca
    48721 gcataaatcg ctcattaata tctagtgccc ctatagaagt caaggcgctt agtccgaata
    48781 gaccaaaatt ctcaaaagtt tttaaatctg tttggattcc tccaccagca aatggatcag
    48841 aaccgccaat tgtcaagatt cttttcataa cattttcctc catgaataag agtataacga
    48901 aattttcaaa aaagaaaaac agccaattgg catattttta tctcatccaa tttaaaaaaa
    48961 caaaaagaaa agaaagacca aaatggatct ttctttcctt aaatagttag gacttttgtg
    49021 attttaattc tttctcgatt tgaagccgtt gcactttcat tgtagctgtt cgcgggattg
    49081 catcaaaatc acggatgata gggacgttca agcgaggaag ttcatggact tgctcccacc
    49141 atcgattcca atccatttct ttatcaggag ctaaagcaat aatcggttgt ggagaatgat
    49201 ttttatccct tacaatcact acttctgcca aaaaatctaa cgaatctaat aaaaagtctt
    49261 caatagctaa tgtactattg atcgtttcga tcaaatccac ttgccgatct ttcaaaaata
    49321 ggtggccatg cttatccata aaaccgtaat ctccgctatc ccaccaatcg ccataaacat
    49381 tttcctggaa ccgtgcatct tccttgtaat aagtcaaagc ccgtccttta gacagtaatt
    49441 gtatatgacc atctgtgttt tcaggtagga cattcccaac tgaatctgta atccgagcgc
    49501 ttgtcatgtc ttccagtcca acgcccatat ctcttgcatc acttgttttc aaactctcta
    49561 gtgtatgagc cttaagaatc attggtccgc attcactttg gccatatact tgaagaaata
    49621 ccgcatcctg tgccatcgac gttcgcaaaa atgtcttcat tgtctggttg tttatagcat
    49681 caaaagtgga gtgatagtat cggatacccg aaaaagcttc tggatgctcc ttagcagtaa
    49741 aacgccactg aacaaagtta ttcggatggg tctctagtgc aattggccgg tgagcttcca
    49801 acatatgaac aacttttgaa ctttgtgcgt ttgctaaagg catcatagga aatcccatgg
    49861 acatcaaaga agagatacca atattgaatc tggaatgcac tggagagatg tgaaaagcga
    49921 ttaatttctt ttccgaaatt tttgtgaaaa ctgttttttg ccatttcgtc cgccatccca
    49981 tagaatgagc agaatgacaa ataagtttag ggataccagt tgtaccagaa gtatgggtca
    50041 tataagaaat ttcatttttg tctaaaaatg attgagaaac agacaaagca ggttgttcca
    50101 ttaaataaag aatagg
//
LOCUS       DAZKSU010000013        46156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000013 DAZKSU010000000
VERSION     DAZKSU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.013"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..921)
                     /locus_tag="ACG8Z3_000770"
     CDS             complement(73..921)
                     /locus_tag="ACG8Z3_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0823111.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="ACG8Z3_000771"
     CDS             complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="ACG8Z3_000771"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0823112.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3422..3601)
                     /locus_tag="ACG8Z3_000772"
     CDS             complement(3422..3601)
                     /locus_tag="ACG8Z3_000772"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0823113.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3648..5471)
                     /locus_tag="ACG8Z3_000773"
     CDS             complement(3648..5471)
                     /locus_tag="ACG8Z3_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0823114.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5749..8028
                     /gene="helD"
                     /locus_tag="ACG8Z3_000774"
     CDS             5749..8028
                     /gene="helD"
                     /locus_tag="ACG8Z3_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0823115.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8121..8768)
                     /locus_tag="ACG8Z3_000775"
     CDS             complement(8121..8768)
                     /locus_tag="ACG8Z3_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823116.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8947..9666)
                     /locus_tag="ACG8Z3_000776"
     CDS             complement(8947..9666)
                     /locus_tag="ACG8Z3_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0823117.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9797..10948)
                     /locus_tag="ACG8Z3_000777"
     CDS             complement(9797..10948)
                     /locus_tag="ACG8Z3_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0823118.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11165..11905)
                     /locus_tag="ACG8Z3_000778"
     CDS             complement(11165..11905)
                     /locus_tag="ACG8Z3_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0823119.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11909..12232)
                     /gene="rsfS"
                     /locus_tag="ACG8Z3_000779"
     CDS             complement(11909..12232)
                     /gene="rsfS"
                     /locus_tag="ACG8Z3_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0823120.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12289..12882)
                     /gene="yqeK"
                     /locus_tag="ACG8Z3_000780"
     CDS             complement(12289..12882)
                     /gene="yqeK"
                     /locus_tag="ACG8Z3_000780"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0823121.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12866..13516)
                     /locus_tag="ACG8Z3_000781"
     CDS             complement(12866..13516)
                     /locus_tag="ACG8Z3_000781"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0823122.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13526..13837)
                     /gene="yhbY"
                     /locus_tag="ACG8Z3_000782"
     CDS             complement(13526..13837)
                     /gene="yhbY"
                     /locus_tag="ACG8Z3_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0823123.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13853..14962)
                     /gene="yqeH"
                     /locus_tag="ACG8Z3_000783"
     CDS             complement(13853..14962)
                     /gene="yqeH"
                     /locus_tag="ACG8Z3_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0823124.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14959..15486)
                     /locus_tag="ACG8Z3_000784"
     CDS             complement(14959..15486)
                     /locus_tag="ACG8Z3_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0823125.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15666..16451)
                     /locus_tag="ACG8Z3_000785"
     CDS             complement(15666..16451)
                     /locus_tag="ACG8Z3_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0823126.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16444..17313)
                     /gene="accD"
                     /locus_tag="ACG8Z3_000786"
     CDS             complement(16444..17313)
                     /gene="accD"
                     /locus_tag="ACG8Z3_000786"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0823127.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17314..18693)
                     /locus_tag="ACG8Z3_000787"
     CDS             complement(17314..18693)
                     /locus_tag="ACG8Z3_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0823128.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18701..19120)
                     /gene="fabZ"
                     /locus_tag="ACG8Z3_000788"
     CDS             complement(18701..19120)
                     /gene="fabZ"
                     /locus_tag="ACG8Z3_000788"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0823129.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19145..19621)
                     /gene="accB"
                     /locus_tag="ACG8Z3_000789"
     CDS             complement(19145..19621)
                     /gene="accB"
                     /locus_tag="ACG8Z3_000789"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0823130.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19624..20859)
                     /gene="fabF"
                     /locus_tag="ACG8Z3_000790"
     CDS             complement(19624..20859)
                     /gene="fabF"
                     /locus_tag="ACG8Z3_000790"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0823131.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20878..21615)
                     /gene="fabG"
                     /locus_tag="ACG8Z3_000791"
     CDS             complement(20878..21615)
                     /gene="fabG"
                     /locus_tag="ACG8Z3_000791"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0823132.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21627..22544)
                     /gene="fabD"
                     /locus_tag="ACG8Z3_000792"
     CDS             complement(21627..22544)
                     /gene="fabD"
                     /locus_tag="ACG8Z3_000792"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0823133.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22651..22875)
                     /locus_tag="ACG8Z3_000793"
     CDS             complement(22651..22875)
                     /locus_tag="ACG8Z3_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0823134.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22928..23890)
                     /locus_tag="ACG8Z3_000794"
     CDS             complement(22928..23890)
                     /locus_tag="ACG8Z3_000794"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0823135.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23900..24340)
                     /locus_tag="ACG8Z3_000795"
     CDS             complement(23900..24340)
                     /locus_tag="ACG8Z3_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0823136.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24646..25611
                     /locus_tag="ACG8Z3_000796"
     CDS             24646..25611
                     /locus_tag="ACG8Z3_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0823137.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25668..25898)
                     /locus_tag="ACG8Z3_000797"
     CDS             complement(25668..25898)
                     /locus_tag="ACG8Z3_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0823138.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25984..26871)
                     /locus_tag="ACG8Z3_000798"
     CDS             complement(25984..26871)
                     /locus_tag="ACG8Z3_000798"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0823139.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26960..28183)
                     /locus_tag="ACG8Z3_000799"
     CDS             complement(26960..28183)
                     /locus_tag="ACG8Z3_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0823140.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28200..29246)
                     /locus_tag="ACG8Z3_000800"
     CDS             complement(28200..29246)
                     /locus_tag="ACG8Z3_000800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0823141.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29410..31137)
                     /gene="ptsP"
                     /locus_tag="ACG8Z3_000801"
     CDS             complement(29410..31137)
                     /gene="ptsP"
                     /locus_tag="ACG8Z3_000801"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0823142.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31137..31403)
                     /locus_tag="ACG8Z3_000802"
     CDS             complement(31137..31403)
                     /locus_tag="ACG8Z3_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0823143.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31882..34116
                     /locus_tag="ACG8Z3_000803"
     CDS             31882..34116
                     /locus_tag="ACG8Z3_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0823144.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34264..34584
                     /locus_tag="ACG8Z3_000804"
     CDS             34264..34584
                     /locus_tag="ACG8Z3_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0823145.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34789..35013)
                     /locus_tag="ACG8Z3_000805"
     CDS             complement(34789..35013)
                     /locus_tag="ACG8Z3_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0823146.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35211..36791)
                     /locus_tag="ACG8Z3_000806"
     CDS             complement(35211..36791)
                     /locus_tag="ACG8Z3_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0823147.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37192..38568)
                     /locus_tag="ACG8Z3_000807"
     CDS             complement(37192..38568)
                     /locus_tag="ACG8Z3_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0823148.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38696..39784)
                     /locus_tag="ACG8Z3_000808"
     CDS             complement(38696..39784)
                     /locus_tag="ACG8Z3_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0823149.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39956..40390
                     /locus_tag="ACG8Z3_000809"
     CDS             39956..40390
                     /locus_tag="ACG8Z3_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0823150.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40468..41001)
                     /locus_tag="ACG8Z3_000810"
     CDS             complement(40468..41001)
                     /locus_tag="ACG8Z3_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0823151.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41095..42273)
                     /gene="mutY"
                     /locus_tag="ACG8Z3_000811"
     CDS             complement(41095..42273)
                     /gene="mutY"
                     /locus_tag="ACG8Z3_000811"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0823152.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42266..43063)
                     /gene="recX"
                     /locus_tag="ACG8Z3_000812"
     CDS             complement(42266..43063)
                     /gene="recX"
                     /locus_tag="ACG8Z3_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0823153.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43170..44552
                     /gene="rlmD"
                     /locus_tag="ACG8Z3_000813"
     CDS             43170..44552
                     /gene="rlmD"
                     /locus_tag="ACG8Z3_000813"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0823154.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44726..45304)
                     /locus_tag="ACG8Z3_000814"
     CDS             complement(44726..45304)
                     /locus_tag="ACG8Z3_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0823155.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45626..46096
                     /locus_tag="ACG8Z3_000815"
     CDS             45626..46096
                     /locus_tag="ACG8Z3_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0823156.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT        13179 a         9801 c         8010 g        15166 t
ORIGIN      
        1 tgatggatca tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa
       61 atagtaaggt ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt
      121 cactttttcg ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt
      181 agcaaattgg gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct
      241 agatgtttgt acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa
      301 cgcaatgatt tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact
      361 tctacctgag tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg
      421 gagcaagacg tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg
      481 cagaatatct tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc
      541 cgattccaaa cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc
      601 ttttatcgct acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt
      661 tgcttctata tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat
      721 tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc
      781 actcaacccg gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt
      841 accgataatg tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc
      901 aatacgacta attacgctca taagttcctc caccctttta atgatagtct atgtaagtat
      961 accatatcag acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc
     1021 caacagaata tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg
     1081 aatacctgtt atctattgtt cggattgctt tctatctact acttttttac aggaaattta
     1141 cctattagtt ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat
     1201 tcaaatcttc tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt
     1261 ccaattcctt gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta
     1321 tcttatagcc cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca
     1381 aacgttcttt gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg
     1441 ttgcttgtat aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag
     1501 cgacaaaacg ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat
     1561 tttccccgtc ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt
     1621 gtatagttga taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg
     1681 gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag
     1741 cggctttcag cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg
     1801 tattgtttgg atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat
     1861 atactgccac catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa
     1921 caaatgtatg accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac
     1981 ctgatttttc atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat
     2041 aagaatggat cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa
     2101 caagcatttc gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc
     2161 agtgtccaga tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt
     2221 aacctagact aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc
     2281 cttttggaag ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa
     2341 gttcttttcc tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt
     2401 ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt
     2461 ttgagtttcc acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc
     2521 cagtggatgg aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag
     2581 taatcacttc ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa
     2641 aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga
     2701 cgattttcat catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc
     2761 cattatctgt atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat
     2821 ttggatagag gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc
     2881 caagtgcatc ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac
     2941 gagtcaaatc aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt
     3001 ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac
     3061 catctggaaa agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag
     3121 atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa
     3181 aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc
     3241 cgaccgccct tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa
     3301 aagtttcagc ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca
     3361 tcaatcaatt atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa
     3421 cttatttttt ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga
     3481 catggatgtg ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca
     3541 tcatattttc taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca
     3601 taatgctctc tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt
     3661 gtcttgggta ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa
     3721 atagagaccg tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc
     3781 gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc
     3841 gctattctca tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct
     3901 ttcaagatga ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca
     3961 ataccgatat ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca
     4021 acaggtcttt gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta
     4081 cgggcatgta cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca
     4141 tccccggtga tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt
     4201 gctgcttctg ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt
     4261 gttacataga ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt
     4321 ccaatcttta cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc
     4381 agttgactaa caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag
     4441 atatggctag attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt
     4501 ccattgactg gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta
     4561 tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg
     4621 atcccgtttt tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc
     4681 agtggacaag gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac
     4741 caccaagaaa taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca
     4801 gctaaacgga caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt
     4861 tgatactggc tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt
     4921 aaagaccctg acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc
     4981 aaagaagatt catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt
     5041 tcgccaggct tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc
     5101 aattttcctt ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt
     5161 tctttacccg ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc
     5221 aatatcatca aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc
     5281 gctaatacat ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc
     5341 aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga
     5401 aaaacaaatt cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg
     5461 aaatgtgtca ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc
     5521 cagctgcctc tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct
     5581 taattgaaaa caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa
     5641 tattcagcaa ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact
     5701 atgctatact tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa
     5761 caactagaac aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg
     5821 ctggatacaa aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct
     5881 aatcacaaga ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc
     5941 caacatgagc aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg
     6001 caaacgttgt ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac
     6061 cgtgaaacag aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca
     6121 atcgttattg actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc
     6181 gcattttatg aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt
     6241 aaaatccaag aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac
     6301 tttttgctag agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg
     6361 atacaaaaag cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa
     6421 ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat
     6481 cataatcgca agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt
     6541 tctgattaca tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca
     6601 ttcaaaaatt atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa
     6661 gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc
     6721 ttagtcgatc agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt
     6781 gatatgaaaa taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa
     6841 gaaacaaatg aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg
     6901 aaaaaattag gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg
     6961 gaagaacagc tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag
     7021 gagcgttcat tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc
     7081 catcgaggga tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta
     7141 caaactgtgc cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa
     7201 catttgacaa ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta
     7261 ctattctttt tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat
     7321 atctttattg atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg
     7381 ttcccaaaag caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa
     7441 acgatcgttg gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta
     7501 acgacaagct atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa
     7561 gaacatcaag tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca
     7621 acagatgata tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc
     7681 cggtattggc gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct
     7741 caactgccag acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg
     7801 aagcgttcca ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt
     7861 ttcttgtggg atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac
     7921 accatgtgta cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct
     7981 ttgttaaaca atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa
     8041 ctgctatcat taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta
     8101 attcaatcat ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt
     8161 aatttgacaa gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca
     8221 aataccttgc tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat
     8281 tcaatgaatt ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc
     8341 tctgatacac gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc
     8401 ttctcatttc tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt
     8461 agaaagaaag cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga
     8521 cgaaaaagca gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc
     8581 atacactcta aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct
     8641 tcaacgccga atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct
     8701 aactcttcta ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt
     8761 gcttccatat tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta
     8821 tgctctcatc ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga
     8881 caatctgtta gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact
     8941 ggttgcttag tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc
     9001 cagtgcatca atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt
     9061 ttgagggatc atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc
     9121 ttcttgtata ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc
     9181 cattacatcg cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc
     9241 ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac
     9301 acccatgttc ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac
     9361 attgttcgtc aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg
     9421 caattcagag tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat
     9481 atgttttggt acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc
     9541 atgcggatca ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata
     9601 aatccggcta ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg
     9661 tcccatattt tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc
     9721 cattatactt gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc
     9781 atttttagtc aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat
     9841 tgcctcccaa ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt
     9901 tttttccgat tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag
     9961 catcggtaaa tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct
    10021 ttttgatatt cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt
    10081 ttgtttttac ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca
    10141 tgcgcgattc tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag
    10201 ataacaaccg ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg
    10261 aaagatcttc ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg
    10321 catggcgaat ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc
    10381 ctgcactttt acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct
    10441 ttgcataact taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat
    10501 acagactgcg aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt
    10561 cttgaaatgc ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt
    10621 aatcaaacga agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca
    10681 agatttttac tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa
    10741 gctccactac taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga
    10801 tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg
    10861 ctcctgttat ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt
    10921 actctacgat aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa
    10981 gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta
    11041 ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc
    11101 gttcggatat ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa
    11161 accgctagtc tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt
    11221 ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat
    11281 aactttccaa tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag
    11341 acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc
    11401 ctggataact atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg
    11461 gaaaaacctc atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt
    11521 ctaacgcttg atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc
    11581 acagcgaatc agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca
    11641 tattgccttc cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca
    11701 tcaacatctc ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa
    11761 ccgctaatgc tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat
    11821 gacgtttcga aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa
    11881 tgaaagcaaa tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg
    11941 gagcatctga ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat
    12001 gtacgatcac atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac
    12061 gttttacttc atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga
    12121 tttgacgttc gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc
    12181 gaacgtctaa agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt
    12241 ttaattgttc tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc
    12301 tacccagtaa ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa
    12361 atgggccaat gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat
    12421 ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata
    12481 aatgattttg tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg
    12541 aagaatctct gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc
    12601 atgccagatt gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac
    12661 cattttgaac tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact
    12721 agctttttca ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc
    12781 aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc
    12841 tctagaataa gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa
    12901 tataatcaat tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt
    12961 gacgtatttt cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg
    13021 gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct
    13081 catcgatttt ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat
    13141 aatctgtatc cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc
    13201 caccacgagc taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt
    13261 ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct
    13321 gataagaagg catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca
    13381 tcactaaatg tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt
    13441 tcttctctaa gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag
    13501 cttgcgtatt catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat
    13561 acttttcttt tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt
    13621 cgcaatctat ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt
    13681 gaagtaacga tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca
    13741 ttgctgaatt gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc
    13801 tacgtaaaaa acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca
    13861 atgctttgcg tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg
    13921 ctgttacagt gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa
    13981 actcaaaacg aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata
    14041 atccacctac atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat
    14101 ttacatcatt tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc
    14161 ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga
    14221 tttcttttaa cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat
    14281 ggcgatggat aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga
    14341 ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg
    14401 tatttttgat gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat
    14461 agacatcccg tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag
    14521 atttcttagc actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt
    14581 ccttcatcca ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt
    14641 tcccaactaa aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac
    14701 cattgaagtc aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat
    14761 tcagtaagcg caaaaagtcg tcatccgtca atgagacatc ttggatctca ttataatggc
    14821 gtaatctgaa acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg
    14881 tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga
    14941 tacattgtaa ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc
    15001 taatagatag gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc
    15061 tgttgcgata atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat
    15121 atcagtcata atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt
    15181 ctttgctatg ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc
    15241 aaatttttca atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg
    15301 gattcctgca tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt
    15361 attccatgca atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt
    15421 taattgggca ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga
    15481 aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc
    15541 gtattatact gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa
    15601 gcatttcttt acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa
    15661 aacaactaat attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat
    15721 cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat
    15781 cgtggcaact gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg
    15841 cgtagttctg gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct
    15901 ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc
    15961 atccagactt catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg
    16021 atgatggcga tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc
    16081 tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag
    16141 gtaatcaccg gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag
    16201 ccttcaggat ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt
    16261 tgaataccga tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct
    16321 tcgtcatctg caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca
    16381 aaaaaatcca agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct
    16441 gtcttatcca cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt
    16501 ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc
    16561 agccttttgg aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc
    16621 agcaaaaccg atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact
    16681 ggcagttact cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt
    16741 gctatgccgt ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc
    16801 ttgcatccgt gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc
    16861 tcgttcgaat aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa
    16921 attaggatcc atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag
    16981 gactgcttca tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg
    17041 aaatgctagc ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc
    17101 aataatcaaa gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca
    17161 tactttttct tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc
    17221 ccacatgtta tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat
    17281 ccgaatatat ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg
    17341 tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac
    17401 tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat
    17461 aaattcactt aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac
    17521 gattattttt gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc
    17581 actgtctact cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg
    17641 agaaggagca aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc
    17701 ttggaatact acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg
    17761 tttttttact aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg
    17821 agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc
    17881 ggcattttca taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct
    17941 ttttgtttgt gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg
    18001 taaagaacaa tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg
    18061 aacttctata tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa
    18121 tgccgctgct gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg
    18181 aactttccga atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc
    18241 aattctgtct gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg
    18301 aatgactggg acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc
    18361 atcgatcgtt gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc
    18421 aaatcggcta ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac
    18481 gattgcagcg ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc
    18541 aatgcagatt gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata
    18601 tatcgctacg gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc
    18661 accgcggtta gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta
    18721 attccgcttc ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg
    18781 aagctttcac tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct
    18841 tacggaattt tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa
    18901 gagaaagtaa tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca
    18961 taacaggttc ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct
    19021 tagcaacgat ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat
    19081 aacgatgtgg aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct
    19141 ttgcttattt ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt
    19201 ctacaaggat ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca
    19261 tcgcttcaat aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag
    19321 ctggtttgtc gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga
    19381 ttccatcaaa tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt
    19441 cagatatgac attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca
    19501 tgcctcgact tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga
    19561 attccgttag cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca
    19621 aaattattcc tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga
    19681 attactcatt gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc
    19741 aatctcagga tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc
    19801 cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga
    19861 tttcgtgctg ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt
    19921 tgcttctgcc tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg
    19981 ttccggctgt acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc
    20041 atctggagta ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc
    20101 tccatagatt ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc
    20161 tgctccttca cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt
    20221 aggatcagtt gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca
    20281 aatcgttgct tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg
    20341 gaatgcttcc ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat
    20401 accttttgca cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg
    20461 tacgaacatt ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc
    20521 gattgttgga agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat
    20581 tttttcttta tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat
    20641 cgaaaacata tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc
    20701 tttgacttca cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc
    20761 gatgccattc gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg
    20821 tgaagtgact ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta
    20881 tccgtgcatg accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg
    20941 tgctaagaaa actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat
    21001 ttgcttttcc gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat
    21061 gaatcctggc gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga
    21121 ttttgttaat cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc
    21181 aatcaaccca gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt
    21241 tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt
    21301 gaaatcttct gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac
    21361 taacacatgg atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc
    21421 ataatcagag atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc
    21481 tatcttttct tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc
    21541 aaaagctaag gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt
    21601 tccttttaca tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc
    21661 ttattctcaa cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat
    21721 acttttccgg ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata
    21781 ctctcataga aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct
    21841 ttcttcatta tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga
    21901 atctgactta ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa
    21961 ggcccgctga catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca
    22021 gcttcgtcta ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt
    22081 gcaggagata cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc
    22141 ggcgcattca tcactgcaac cattttcccg cttccagctg gggtagcttc tgtcatataa
    22201 gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact
    22261 aatgccgaat attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc
    22321 ttcaataagc gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc
    22381 tgatccaaac gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca
    22441 cttgcttcat caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct
    22501 tgatattgtg caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata
    22561 tccagaatat atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa
    22621 atgtcccagc ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac
    22681 taaatcttga accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc
    22741 aatttcattg atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc
    22801 agtagtcaat ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat
    22861 tttttcgaat accataataa tatattcctc cgatattgtt tgctttttat ttttataatt
    22921 taacagtcta caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa
    22981 atattacttt ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata
    23041 agatagggat gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt
    23101 cttgcggcat ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca
    23161 aaagcagata atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc
    23221 gtacggcaaa atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat
    23281 cttctgaata gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg
    23341 taccgtcagc tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa
    23401 tcccacctgc tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct
    23461 tagatagtgt ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt
    23521 cagccatgct taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag
    23581 catgagttgc tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg
    23641 gcgacattgt tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta
    23701 tcaatttttc cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc
    23761 gttcacgaat acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa
    23821 gatcattatt agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa
    23881 attgttccat taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg
    23941 catccagcaa ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt
    24001 cacgatgaaa atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact
    24061 ttacaacgcg gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact
    24121 tgttaatagc agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag
    24181 ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat
    24241 ctttgaattg agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac
    24301 taacaagata atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta
    24361 gtttgattat caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc
    24421 ttaaaagatc atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac
    24481 tgataaatca gcatttataa tttcttttat tagaaaaatc atctatctca catattttga
    24541 ttatcaaagt attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc
    24601 aaaatttgtt atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa
    24661 atgaaaactc tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc
    24721 atctatgttg gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat
    24781 aaacagcatt caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc
    24841 gtagtagaca cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt
    24901 cctttttata accgacaaat gaattatttc agtaattata attatgtaca tattaccaat
    24961 aagggatttg tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat
    25021 aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct
    25081 gaaattagtt tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa
    25141 cagaaaaaaa aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac
    25201 ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt
    25261 caaaaagata tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt
    25321 ttaagaacac catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag
    25381 cactacctcg tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct
    25441 cctgacttct attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa
    25501 gaagaaatcg atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag
    25561 tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc
    25621 ggcttctttt aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag
    25681 tcatagattt ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct
    25741 gtttcagtta attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg
    25801 ccttcttttt caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc
    25861 agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt
    25921 tctgttatat tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt
    25981 tttttatgat tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg
    26041 ggttccgtca ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt
    26101 agttgctggc aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt
    26161 gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata
    26221 attgcttaaa gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa
    26281 atcaagtccg tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc
    26341 aatcatgatc tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt
    26401 aaatgtcgta ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc
    26461 accgaccatg atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc
    26521 atctaacgca tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg
    26581 tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga
    26641 ctgtccatag tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt
    26701 actttgttct gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg
    26761 atcagctttt gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg
    26821 gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac
    26881 ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt
    26941 cataaaaaat gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa
    27001 tcgagtcttc ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat
    27061 aaaattcgat catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta
    27121 aaacttttgg atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt
    27181 cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag
    27241 catttccttc cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt
    27301 gtgtttctga agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat
    27361 cattgttgtc cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca
    27421 attcctctaa ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat
    27481 aagcggagat gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa
    27541 tcctgcttaa agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt
    27601 tgctgatatc ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag
    27661 gaatcacgcg aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac
    27721 ttggacaaac tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg
    27781 attgcctgat cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt
    27841 aagtatggat cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc
    27901 cgaattctgt atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat
    27961 accacatgcc tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca
    28021 ttcgaatcac atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga
    28081 cttcatgccc atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc
    28141 tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac
    28201 tagtccttga cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt
    28261 tcgtatacat ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga
    28321 taaccggctt gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata
    28381 aattcttcgt ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac
    28441 actggaatat ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc
    28501 ccttctgtct cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct
    28561 atgatctctc cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca
    28621 attttccgga tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg
    28681 tattcgggca tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac
    28741 aaaccgacac aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt
    28801 tgttctttca ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga
    28861 atcggcacac ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc
    28921 agatagtccg ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg
    28981 ttcgatccga taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact
    29041 ttttttcctt ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg
    29101 atgtgcagga tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc
    29161 agagcacgtt tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca
    29221 ctttcaaaat ataataatac cttcacctta cgtccccctt cacttatata gtataacata
    29281 gcctacaaac tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg
    29341 ttttcttaaa aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg
    29401 atacgttgtt tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca
    29461 ttctttcaat gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa
    29521 actacgcgtt ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac
    29581 tagcaatgga acagctgttt gatctccagc catttctcca cacatacctg cccattttcc
    29641 ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg
    29701 ttggtacaag taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa
    29761 atcgttcgta cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc
    29821 tgctgctgga atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc
    29881 acttatcaat ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag
    29941 tgtagctacc atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc
    30001 acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg
    30061 atatcctaag aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc
    30121 accgatgtcc attgtacgga caactacagg ttttccttcc ataccttcta atacagcttt
    30181 gtaagcttca tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc
    30241 agtacggtac agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg
    30301 tgttccgatg tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt
    30361 ttttaatttt tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc
    30421 agctttttgt tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa
    30481 aatatcgcct tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc
    30541 taaagaacgt gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc
    30601 ttttacaaaa ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac
    30661 gaccacttct tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa
    30721 aatacgttta gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc
    30781 cattgcttcg aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt
    30841 tacgctgttg tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac
    30901 catcaaatgg gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc
    30961 acgaatttgc tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc
    31021 agccgcagta tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta
    31081 agcttttgca acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat
    31141 tcagataagc cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca
    31201 gcaccttctg cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc
    31261 atgattgatt tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat
    31321 ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact
    31381 acgtgaaatt ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg
    31441 tgagggtttt cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta
    31501 aagattaaca ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat
    31561 tcaaattatt ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat
    31621 ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct
    31681 ttgttttcat atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca
    31741 aaggtcagga atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac
    31801 taggggttat tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt
    31861 gtgaaaggac gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt
    31921 cacttgtatg ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat
    31981 caaaaaatca aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt
    32041 ggatttggca gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga
    32101 aattatgagc aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag
    32161 ccacctcaag gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa
    32221 gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt
    32281 gaaattttaa atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt
    32341 aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa
    32401 ttaatggaca aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata
    32461 cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat
    32521 gtgatcctat tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga
    32581 aatatggatg ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt
    32641 ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt
    32701 atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt
    32761 ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca
    32821 gccgcaactt tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat
    32881 ttgctagatg aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca
    32941 atcgagaaaa aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat
    33001 ttcgaaaaag cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt
    33061 cagctcactg aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa
    33121 caacggacag gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac
    33181 ttagcagatg acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg
    33241 aaagcgattc gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc
    33301 ttatttgttg gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa
    33361 ctattcggtt cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat
    33421 agtgtctcta aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa
    33481 ttaacagaaa aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa
    33541 gctcatccag atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat
    33601 gcacaaggaa gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga
    33661 actggtaaag tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct
    33721 gtattgaacc aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc
    33781 atcgaattca gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac
    33841 gatgtcaacc aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa
    33901 gaaaaattag ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg
    33961 atccaagaac aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat
    34021 gagttgaaag caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt
    34081 ttggctaaag aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg
    34141 tatttctttg agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt
    34201 tataaaaaag aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc
    34261 gccatgaaat tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa
    34321 aacactgatg cccatctagt caaggtctac acagccggaa ataccactat tgtctacact
    34381 gaagcccctg aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa
    34441 gaaatcgaag atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag
    34501 gatgaaatca aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa
    34561 ttaggcgcta cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac
    34621 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcga aagcagaagc
    34681 aactccttcg gaaataagct gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc
    34741 agcttatttc ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga
    34801 tttttctctt ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt
    34861 tttaactttt tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt
    34921 gtcccagtct tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt
    34981 ttttctttca cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat
    35041 atgtacgcaa aataattttt atattttttt gattagacat ttctatgatt ttaatatagt
    35101 aaaaagagcc gtcacggaaa atccgtcacg gctctaaaca aaatatgaga ggctgttttt
    35161 gtttggctat tcttattact tgatgcaggg cagcttgtgt caagacttct ttacatcaaa
    35221 ctttttaatt ttacattggg atatttatcc gcaaaccagc gttctgcaaa ttggttctcg
    35281 aataagaaca acggttggtc gaaacggtca cgagccaaga tattgcgact tgaactcatc
    35341 ttctcatcca aatcttctgg atcgatccag cgagcgattt tgtgtcccat tggagacatg
    35401 attacttcgg cgttatactc atttaacata cgatattgga atacttcgaa ttgtagctga
    35461 ccaacagctc caatgatata ttcttccgtt agatatgttt tataaagctg aatcgctcct
    35521 tcttgaacta attgatagat tcctttgtgg aaggattttt gtttcatgac attttttgca
    35581 gtcactttca taaacagctc tggagtaaat gaagggagtt cttcataagc aacttttagt
    35641 tttccttcga acaatgtatc cccaatctga taattgcctg tatcatatac accgatgata
    35701 tcgcctgcta ctgctttttc gacgttttct ctcgaatctg ccataaattg tgtgacattg
    35761 cttaatttca gttttttatt tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt
    35821 ccagaacaga ttcggacaaa agcgattcgg tcacgatggg ctggattcat atttgcttgg
    35881 atcttgaata cgaaacctga aaattcttcc tcataaggac ttaccatctg atcatcttgt
    35941 gttttgtgtc cataaggagc tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca
    36001 ccgaagttag tcaatgcaga accaaaaaat actggtgtct gatcgccacg agcaattttt
    36061 tcttgattaa atgcatctcc agcctctttg attagttcta cttcttctaa gacttgttca
    36121 tataaggaat tttcagttag aggatgattt tcgggaagca gtccatcttc atccaattct
    36181 actaatcgtt catcctgata attttccgga cgataaaact ctacacgatt atggtagata
    36241 tcataaagac cttctagccc tttacccata ccgattggcc aattcatcgg ataagattcg
    36301 atatctaata gctcttctaa ttcttctaac aattccaatg gttcacgacc atcacgatct
    36361 agtttgttga taaaagtaaa tatcgggatg ccacgttttt ttacaacttg gaataatttc
    36421 tttgtctgtg cttcgatccc ttttgcacta tcgattacca tgaccgcact atccacggcc
    36481 atcaaagtac gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt
    36541 actcgtttcc cttgataatc aaactgcata acagaactag taacagaaat cccacgttgt
    36601 ttctcgattt ccatccagtc tgatttggca aaattacctg tttttttgcc ttttacggtc
    36661 ccggcttgac ggatagcccc accaaacaat agtaattgtt ctgtaatcgt tgttttccca
    36721 gcatccggat gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt
    36781 ggattattca taacctaact ccatttcttc cattcaatta tttatatttt ctctaggcac
    36841 agattgtccg atctcttaaa gaaagacaat ttcatatagg gctgtgacaa aagccgtgta
    36901 gcagtctctt tttccgagta aacggcgttc tagagacagc ctatcagcaa taagcccacc
    36961 caagccaaaa atacgaaaag ctgtttttgc ttggctgctc ttattactta atgcaggata
    37021 gctttttcac aacctttgtc aactactaca actgaatcag tatagcatgg attcagcgaa
    37081 tgaaaaagcc accaaaccat aagagtcaaa tatttttttg tcagctgact aaaagaaaag
    37141 acgtgttgct tgtttattca agcaacacgt ctttcataac caagtttcca attatcttct
    37201 tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag gttccgcttc
    37261 tgtttcttcc ggatcatgaa aaatgacacg gattttaagg attcgagaac cttccatttc
    37321 ttcagaaacc agcgtaatat tatcgacatc aaacgataac ttttcgcctt catccggaat
    37381 catgcctaat gctgtaatca aataaccagc catcgtatct acatcactca tatgcagatc
    37441 actgtcaaaa gcttcattga actcatcaat cagcatacgt ccttggatga tatattcgtg
    37501 ttcatcgatt ttttcataaa gattctcaac ttcatctgat tcatcatcga tttctccgac
    37561 gatttcttct aaaaggtctt ctagtgttgc taacccaacg acgccgccat attcatctaa
    37621 taaaatagcc atctgattct gcgtcttttt catttcatat aacagatcat caataaagac
    37681 cgtttcagga acgaataaag gctcctgcat gattttcttg atatctaaat tatcaaagcc
    37741 gaatttatgg gcagctttta gtaaattctt tgtatgaagg attccgacta ctttatcttt
    37801 gtcttcgtta taaacaggga ttcttgaata gttttcggat aaaacttcat tcacattttc
    37861 ttccacagtg tcgttgatat caatcataaa tgcatccgtt cgtgggacca tgacttctcg
    37921 ggcaactttc gtatcaagag aaaaaacccc ttggagcatt tctaattctt cattattcaa
    37981 tacgccttcg ttttcaagca tataacgcat ttcatctctg gtcatttttg aatcttcatc
    38041 atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa gcagccagac
    38101 gaagggctta gctactacac caatcaagcg tatcagccct gaagtgaact ttgcgacttc
    38161 ttctgattta tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa tcgatacata
    38221 ggtcaaaata gccagcacaa tgatatttgc aatatttttt gccgctgtgc cgccacctaa
    38281 taccggagct aaccgttccg ataaagtatt agctagtgaa gcaccagaca aaatgttgac
    38341 caaagtgatt cctacttgga ttgtcgatag gaaattattt ggattctgca atatttttaa
    38401 aagcttttga gatttcacat ctccttcatc agctttttgc tcgattcgat tcttattgat
    38461 cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg atcaatgtca aaacgatcaa
    38521 taataaaatc tgcgctaaca gcgactgact ctcagggtca gcattcataa tcgcattctc
    38581 tccttataaa attaattttt agtttacgat agaaaaaaac gtggcactat agccacgtta
    38641 aaatggtatc acaaagcaat tttctttgca ataaaaacaa gtgtactaaa actacctacg
    38701 aggtcgtagc aattttcttc ttgttttcat tttgcttgtc ttctttgacg attttgatta
    38761 tataggaaat gatcgtcttg cagattgcgt aaaaaggaac acctaagaag ataccaagca
    38821 atccggcaat atttccagct actagaagaa cgataatgat cgttaatgga tggattttca
    38881 gtgatttccc aattacatta ggataaatga tattcccatc caactgttgg acgattagca
    38941 caaccacaca acaaagtaat gcttttaccg gatcattgaa gactgttact agaaatgcag
    39001 gtgctagtcc tagataaggt cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc
    39061 caaataagaa agcataatcc acaccaatta ccatatatcc taaaaatgtg aaggtaccaa
    39121 cgaataaaca ttcgattgct tgtccgctaa tgtaattagc caatgtttta ttcagttgcc
    39181 ccaataactc cacaatttgg tttcttcttt tttctgggaa aaactgttgg atgtttggca
    39241 ctagtttttc tccgtctttc aacatataga acaagatgaa tggtgctgta atgattacta
    39301 cggttgccga agcgatcgtt ccaacgatcg aacctaaact attggatact ccactaagga
    39361 attgctgaat gattgtcccg taagaaatgt ctaattgctc aaaatattta ttgatatcta
    39421 cttccttgaa caaaggcaat tcggctacct cttttaacca gctttctacc tgtttgatga
    39481 aatcaggcat attagatgcc aatgaggaaa tttgctgtac tagacttggg atgacactaa
    39541 gtaaaatcca aataattgct gcaactaaaa ggagcagcac aattgcgaca gcccatattc
    39601 ttttgatttt tgctttcatc aacagcttga caataggatt caataaatag taaagaaagc
    39661 ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa
    39721 agtttatttc tgatgaaatc atgatcaatg cagctatgat caataattca agcgaccaaa
    39781 acattagttt agactgtcta agtttttcaa acaatagatt tcctcctttc tttcttacta
    39841 gtatatatta tattcgttaa aaattctttt attctttttt tatttttgaa aacttgccat
    39901 actttttatt tgttttattt tccagtatac tatacgaaag gaaggtggct agaccatgaa
    39961 agttgtacaa acaaaagata caatgagcaa tatttactta gatgctgtac ggatcaggca
    40021 tcaagttttt gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca aagatgaagc
    40081 ccattgtatc cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct
    40141 tttgccttta gaaaacggaa aaatgaaatt acagcggatg gcgattcttt ctgaatacag
    40201 acaccaaggg ttaggaaaaa tattgattga agaagcagaa aactttgcta agaatcaagg
    40261 ctataatacg attttattag gggcacagtc cacagctgaa actttttacg aaaaactagg
    40321 atatacagct tacggcgatc cttttgaaga tgctggcatg cctcatattt atatgaaaaa
    40381 gactttataa aaatttattt cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc
    40441 tcactggctt actgttcttt tttgtgttta cttacgcgac aattgcttgt aacggttata
    40501 ccaaatatca atatagcctt ccgagaacgg gccatctcca ttattgatcc agtcaaccag
    40561 aatcttcaca ttttctttta ggatgaagtc aatatcgttc gggtaattca tcattttgct
    40621 gtgcatctca tattcatcga catccaatag tcgtttctcg ccgtcaggaa ataccttgat
    40681 atccaagtca tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag
    40741 attacaataa taggaaaccc ccttttctct gatcattgct attacattga accagtattt
    40801 tttatgaaaa taaacaatcg ctggttcgcg cgttacccat cggcgtccat cagattcagt
    40861 gactaatgtg tgatcattca cgccgatcat agagtgttcg cttgttttta ataccatggt
    40921 atctcgccac gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc
    40981 tccttctttt ggaactccca tagcaaccct tccttaagca gcattttgtg ctttgtgttt
    41041 gggtgttcat tacaccgaca tcataattat tatatcaata aaaatgagct ttgcctagaa
    41101 aggattccta ttgtttcgat ttttctctaa ctgatctacc agtttcattt ggaccttagg
    41161 aaaaacgacc gaatcaaatg catcaggctt caaccacttc gaagttttgt tctcggtaaa
    41221 ttcttgttct gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg
    41281 actaaaaata tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt
    41341 ttcaaatggg agattttgct cttcttctgc tactaggtca tcaaacagat cgatttctgg
    41401 ctttgtttcc cattcttttt tcaatcgctc atactcctct tgtgtcactt ccatcattgg
    41461 aaatgtccac atattcgcca gcaatttttg gctatctctt tcttcaaaat aatacgctcc
    41521 agaatgattt tgaagagctg cactgatata ataaacgtct tttggctttg ctttttttgt
    41581 ttttacagga aaactcgttt ggattcctcg tttgttagct aaacaaaagg cttggatcgg
    41641 acaagtctca cactttggag aagttggtgt acagatagct gaacctagat ccatcattgc
    41701 ttggttgaat tcacctggat gcttttcatc aatgattttc cgcatcgctt catcaaagat
    41761 tttcctgcta gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg
    41821 catcacgttg ccgtcaactg caggttctgg aagtccaaat gcgatacttg caatcgcgcc
    41881 tgtcgtatat ggtcctattc ccttcaatga gctaatttct tcaggcgttt gcggcatttc
    41941 cccatcaaat tctgacatga tctgtttagc agcagcttgg atgttccggg ctcttgagta
    42001 ataaccaagg ccctcccatg cttttaaaag cttttcttct ggagcatttg ctagctcttc
    42061 aattgtagga aaccattcca tgaaacgata aaaataatcg atcactgtgt ctactctggt
    42121 ttgctgaagc atgatctcag agatccagat acgataagga tcacgattat agcgccaagg
    42181 caggtttctt ttttcttgtt cataccattg aatgaactga tcttgaaatt cttttgtttc
    42241 tttatctgtc catttttccc attctttatt cactttcctc ttccttttcc gtaatgaatc
    42301 gctggatcat atccaaatca aatccttttt gatataagct ttgctttact tttagatttc
    42361 tttttttagg ttcaaacctt aaatttcttc gccatagttt atctccttgt ttgactaaat
    42421 aatcatattc tatttcttga tctgcttctt gcggaagatc agctatcgct tgttggatca
    42481 cttcttgaga aaagcctttc gtcatcaatg tttgttggat ttttctcaat aattcttttt
    42541 ggctttttcc gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt
    42601 ggtcttcaaa tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt
    42661 gctgcatctg ctgcgctaat ttctttggtc ctttgtcact taagcgcata ttcgtgcgca
    42721 cataactttc cccatagacc aaatcatcga caaggtccaa ttcttttaag cgggctacga
    42781 ttttatttcg atcttcttga aggatttcct tttcttttaa gtatgttcgg atttcttttt
    42841 ccgatcgcaa ctgataactt aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc
    42901 tactcttctt gatctcttgg atagtttctt cgtctaattc ccgtcctttt aataatcgat
    42961 agcgtactaa tacatcttca gatacttgta aagcttctcc ttcagataaa tctacgcgat
    43021 agaaagggcc tttgccttta ctgattctcg taacggttaa cataaacatc ctcctcgaac
    43081 atatattctc tatcatacca cagaaaatcc ccgtaatcag gtattatatg ttagaataaa
    43141 ccacagaaaa aagaaaaagg gagatactta tggcagaaac aattgtcaaa actggacaaa
    43201 cggttccttt gaaaatcaag cgtttaggca taaatggtga aggaatcggt tatttcaaac
    43261 ggttgatcat atttgttcca tatgcactac caaaagaaga agtactagtg aaaatcacga
    43321 aagcaactcc tcgctatgca gaaggagaat taataaaagt caaaaaaaca agcaaagatc
    43381 gcgtagtggc accatgtcct gtttactatg aatgcggtgg ctgtcaatta caacacttag
    43441 cttatcaagc tcaattggat tttaaaaagg atctgctttt gcaagcattg gaaaaattca
    43501 aaccagcagg ctttcgatct tatcaacttt tgccgacgat cgggatggat gagccttggc
    43561 attatagaaa caaagcacaa tttcagttac gaaaaaataa gcagactcaa aaaattgaag
    43621 ctggtttata cgaagcaaac tctcatcagt tagtccctat aacagactgc ttggttcaag
    43681 aacctcgaac acaaaaagta atgaatactg ttgttcaact attgaataaa tttgatgcac
    43741 ccatctatga tgaacggaca aacagtggaa tcttccgcac aatcatggtt agaatcggtg
    43801 ttaaaactgg ggagttacaa gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa
    43861 acgcaatgat ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg
    43921 tccaggcaca aaaaacgtct ctcgtgatgg gggatgaaac gatccactta tggggaaaag
    43981 aagccattga agaacaaatc aatgaagtga ctttcgatct ttctcctcgt gccttctttc
    44041 aactcaatcc tcagcagacc gaattgcttt atggcgaagg aatcaaagca ttagatgtcc
    44101 agcctaatga aacgatcgtg gatgcttatt gcggagttgg gacgatcggc ttaagtgtag
    44161 caaaacaagc caaagaagta cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa
    44221 aaaaaaatgc ggaaagatta ggatatcaca acactcgata tgaagtcggg acagcagagc
    44281 aagttctgcc taaatggttg aatgaaggtt ttcagcctga tggtatcatc gtagatccac
    44341 cacgtacggg tttggataac gcattgatcc aatcattatt gaaataccct ccaaaaaaac
    44401 ttgtttatat ctcctgtaat gtttctacac ttgccagaga tctggttaat ttaagtaaaa
    44461 agttcaagat tgaatattta cagtccgtcg acatgtttcc tcagactgca cgttgtgaag
    44521 ttgtcgtgaa gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc
    44581 tgtatcaagt aataagaaca gccaaacaaa aatagctctt catgttttat gaatactgta
    44641 cttgtgaccg aagagttaac aggaggacac tatttattta aaaaaagggt tgtgacatag
    44701 tttttgtcac agcccttttt gattattatt ggatattcac gtctatcact tgataaaatg
    44761 catttgtcgt atcgctgata ttccatactg ctaaaatcac attatatcct tttcgatcct
    44821 gcggaagtgt gatcgtttgg ttgactgttt ttggtggaac agttgctttg tcgtctattt
    44881 ttgtgatcaa ttcaaaatca ctaaatttca aaggttgatt aggattccaa gttggtttgg
    44941 taatgtagta atcccaagta cttgtccgat gttgtgctgt taaattccaa gtaatattta
    45001 atgttcctgg attaaccatg tttttatgcc atctgttaga tgtctgttca tctaaaggct
    45061 caaagccgct gactcctgca ctagcaattt ttccatcaat gaatgtattt ttggtagctt
    45121 cgatactttg cggttcccat tgtgctcttc cgacattttg atttaagttt cctgctgctg
    45181 atgttcctag gtatgcacgg cttcctggag aagtaatata accatgcgca aaacctggaa
    45241 tgctaaaacc gaacaaccct aaactaacta acccaataaa caataacgta ctaatctttt
    45301 tcatgtagaa acctccttca tttgataacc acattgtaaa cgctttattg tattaacaca
    45361 aacattatgc ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga
    45421 ttataacaga agtagacgag aaaagtacaa atagaaaaca tgagaatcaa atcatatgat
    45481 ctttttcaaa aacatcattc atataaacaa atgttcaact ttttaataac aacaattatc
    45541 aatttttttc ttacttcaca aactaaatac aggtcttgct gtgagccttc atggtaaact
    45601 aatattatag aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg
    45661 tgttatagga attgtcatat taggcttttt atcagggttg cttagcggaa atcccggaaa
    45721 atattactat tctttgcaac ttccaccatt tgcaccacct agctggatat ttgggccgat
    45781 gtggacctta ctgtatatat tgatgggtat ttctctttat ttgttactta atcacaacaa
    45841 caaaaaacag cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt ttaattttat
    45901 atggtctgct ctatttttta atttaagaaa tattttcatt gcagcaatcg atattacact
    45961 tttagttatt ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat
    46021 gtggctcatg attccttatt atttatgggt actattcgca actctattga attattcaat
    46081 ttatttttta aactaagcgc taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg
    46141 ccacctaagt tagatt
//
LOCUS       DAZKSU010000014        44620 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000014 DAZKSU010000000
VERSION     DAZKSU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.014"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(261..731)
                     /locus_tag="ACG8Z3_000816"
     CDS             complement(261..731)
                     /locus_tag="ACG8Z3_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0823157.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            1061..2701
                     /locus_tag="ACG8Z3_000817"
     CDS             1061..2701
                     /locus_tag="ACG8Z3_000817"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0823158.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2855..4177
                     /locus_tag="ACG8Z3_000818"
     CDS             2855..4177
                     /locus_tag="ACG8Z3_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0823159.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4245..4679)
                     /locus_tag="ACG8Z3_000819"
     CDS             complement(4245..4679)
                     /locus_tag="ACG8Z3_000819"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0823160.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4744..5580)
                     /locus_tag="ACG8Z3_000820"
     CDS             complement(4744..5580)
                     /locus_tag="ACG8Z3_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0823161.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6322..6948)
                     /locus_tag="ACG8Z3_000821"
     CDS             complement(6322..6948)
                     /locus_tag="ACG8Z3_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823162.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7243..7578)
                     /locus_tag="ACG8Z3_000822"
     CDS             complement(7243..7578)
                     /locus_tag="ACG8Z3_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0823163.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7565..7849)
                     /locus_tag="ACG8Z3_000823"
     CDS             complement(7565..7849)
                     /locus_tag="ACG8Z3_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0823164.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7905..9428)
                     /locus_tag="ACG8Z3_000824"
     CDS             complement(7905..9428)
                     /locus_tag="ACG8Z3_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0823165.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9421..10596)
                     /locus_tag="ACG8Z3_000825"
     CDS             complement(9421..10596)
                     /locus_tag="ACG8Z3_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0823166.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10600..10785)
                     /locus_tag="ACG8Z3_000826"
     CDS             complement(10600..10785)
                     /locus_tag="ACG8Z3_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823167.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10751..12445)
                     /locus_tag="ACG8Z3_000827"
     CDS             complement(10751..12445)
                     /locus_tag="ACG8Z3_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0823168.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12442..12915)
                     /locus_tag="ACG8Z3_000828"
     CDS             complement(12442..12915)
                     /locus_tag="ACG8Z3_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0823169.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12984..13139)
                     /locus_tag="ACG8Z3_000829"
     CDS             complement(12984..13139)
                     /locus_tag="ACG8Z3_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFQ0823170.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13273..13653)
                     /locus_tag="ACG8Z3_000830"
     CDS             complement(13273..13653)
                     /locus_tag="ACG8Z3_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0823171.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13657..13872)
                     /locus_tag="ACG8Z3_000831"
     CDS             complement(13657..13872)
                     /locus_tag="ACG8Z3_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823172.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13875..14282)
                     /locus_tag="ACG8Z3_000832"
     CDS             complement(13875..14282)
                     /locus_tag="ACG8Z3_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFQ0823173.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14548..16023)
                     /locus_tag="ACG8Z3_000833"
     CDS             complement(14548..16023)
                     /locus_tag="ACG8Z3_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFQ0823174.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(16013..16861)
                     /locus_tag="ACG8Z3_000834"
     CDS             complement(16013..16861)
                     /locus_tag="ACG8Z3_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFQ0823175.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16898..17260)
                     /locus_tag="ACG8Z3_000835"
     CDS             complement(16898..17260)
                     /locus_tag="ACG8Z3_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823176.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17261..17419)
                     /locus_tag="ACG8Z3_000836"
     CDS             complement(17261..17419)
                     /locus_tag="ACG8Z3_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823177.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(17560..18184)
                     /locus_tag="ACG8Z3_000837"
                     /pseudo
     CDS             complement(17560..18184)
                     /locus_tag="ACG8Z3_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18181..18492)
                     /locus_tag="ACG8Z3_000838"
     CDS             complement(18181..18492)
                     /locus_tag="ACG8Z3_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823178.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18536..18829)
                     /locus_tag="ACG8Z3_000839"
     CDS             complement(18536..18829)
                     /locus_tag="ACG8Z3_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823179.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            19023..19670
                     /locus_tag="ACG8Z3_000840"
     CDS             19023..19670
                     /locus_tag="ACG8Z3_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0823180.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19731..20876
                     /locus_tag="ACG8Z3_000841"
     CDS             19731..20876
                     /locus_tag="ACG8Z3_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0823181.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20957..21262)
                     /locus_tag="ACG8Z3_000842"
     CDS             complement(20957..21262)
                     /locus_tag="ACG8Z3_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0823182.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21287..21718)
                     /gene="ruvX"
                     /locus_tag="ACG8Z3_000843"
     CDS             complement(21287..21718)
                     /gene="ruvX"
                     /locus_tag="ACG8Z3_000843"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0823183.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(21715..21981)
                     /locus_tag="ACG8Z3_000844"
     CDS             complement(21715..21981)
                     /locus_tag="ACG8Z3_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0823184.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(22236..22961)
                     /locus_tag="ACG8Z3_000845"
     CDS             complement(22236..22961)
                     /locus_tag="ACG8Z3_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0823185.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(23322..23392)
                     /locus_tag="ACG8Z3_000846"
     tRNA            complement(23322..23392)
                     /locus_tag="ACG8Z3_000846"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23358..23360),aa:Gly,seq:ccc)
     gene            23509..24261
                     /locus_tag="ACG8Z3_000847"
     CDS             23509..24261
                     /locus_tag="ACG8Z3_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0823186.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            24277..24756
                     /locus_tag="ACG8Z3_000848"
     CDS             24277..24756
                     /locus_tag="ACG8Z3_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0823187.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(25007..26587)
                     /locus_tag="ACG8Z3_000849"
     CDS             complement(25007..26587)
                     /locus_tag="ACG8Z3_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0823188.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(26580..27323)
                     /locus_tag="ACG8Z3_000850"
     CDS             complement(26580..27323)
                     /locus_tag="ACG8Z3_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823189.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(27499..28275)
                     /locus_tag="ACG8Z3_000851"
     CDS             complement(27499..28275)
                     /locus_tag="ACG8Z3_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0823190.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(28523..29359)
                     /locus_tag="ACG8Z3_000852"
     CDS             complement(28523..29359)
                     /locus_tag="ACG8Z3_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0823191.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(29370..30299)
                     /locus_tag="ACG8Z3_000853"
     CDS             complement(29370..30299)
                     /locus_tag="ACG8Z3_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0823192.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            30491..30844
                     /locus_tag="ACG8Z3_000854"
     CDS             30491..30844
                     /locus_tag="ACG8Z3_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0823193.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(30918..31574)
                     /locus_tag="ACG8Z3_000855"
     CDS             complement(30918..31574)
                     /locus_tag="ACG8Z3_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0823194.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            31800..33734
                     /locus_tag="ACG8Z3_000856"
     CDS             31800..33734
                     /locus_tag="ACG8Z3_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0823195.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(33820..35043)
                     /locus_tag="ACG8Z3_000857"
     CDS             complement(33820..35043)
                     /locus_tag="ACG8Z3_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0823196.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(35037..36908)
                     /locus_tag="ACG8Z3_000858"
     CDS             complement(35037..36908)
                     /locus_tag="ACG8Z3_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0823197.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(36929..38047)
                     /locus_tag="ACG8Z3_000859"
     CDS             complement(36929..38047)
                     /locus_tag="ACG8Z3_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0823198.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(38130..38963)
                     /locus_tag="ACG8Z3_000860"
     CDS             complement(38130..38963)
                     /locus_tag="ACG8Z3_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0823199.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(38956..39872)
                     /locus_tag="ACG8Z3_000861"
                     /pseudo
     CDS             complement(38956..39872)
                     /locus_tag="ACG8Z3_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(40099..41223)
                     /locus_tag="ACG8Z3_000862"
     CDS             complement(40099..41223)
                     /locus_tag="ACG8Z3_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0823200.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            41406..42314
                     /locus_tag="ACG8Z3_000863"
     CDS             41406..42314
                     /locus_tag="ACG8Z3_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0823201.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            42455..44068
                     /locus_tag="ACG8Z3_000864"
     CDS             42455..44068
                     /locus_tag="ACG8Z3_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0823202.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        13487 a         8893 c         7441 g        14799 t
ORIGIN      
        1 agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg
       61 tcagaacgtt gacttcacag tctcttcctt aaacggtgtt caaaaacttg catctgcggt
      121 aataagccca accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
      181 tgatgcaggg caggtttttc acgacctctg attcacggtg tttctttttc atcaacattt
      241 ttacgaaata ctgtggaaga ctattgaaat aagtgtggat tgtttctttg tgtttcaatc
      301 gtttcttgcc attcttcatt taattttgga ttaccgtttt cttccttgaa ctcataaggt
      361 cctaattcaa aaatcccaga cagtatttga ttcacgattt catatagttc agtgtgatca
      421 gacacatgat tcttgaagat catctcatta tcaccacgtt tgaacctcac attcgggctc
      481 tcatgatgaa cctctatgca tttttctcct ttttttaact gacggacttc tgaaaacatc
      541 tcataaatat cttctccagt aaaaccttca gcaagcgcca gactgtccac aagaccgcct
      601 actgtcagtt cagagattat tgcttgattg gaatctaatt ttaatgtata gcttgtcgct
      661 tctactacgc cacatgcatt gcaaccgcct tctgatagtt cgctttttct aatcaattga
      721 acttctttca ttttctcttc ctcctcttaa ttttctactt atgtattttt atcacctgac
      781 ataggtgact cctatcaacg ctatcatgtg cttttttcct cagaaatcct atttatccat
      841 tccatctatc ccttattctt ctacttttct ttctctagca tataagagat acgcatagta
      901 ttcaagcaag gattgtcgat ataaaaagaa caaatactgc tattttgatt tcttttggct
      961 atattacata taaataaaat ttatcatacc tgctcttttt gactatatca tcatctgact
     1021 tctatacttc gtttgtatta aaacttagga ggaatcattc atgcttacag ggttaaactt
     1081 attaaataat ccttttctaa ataaaggaac agcttttaca aaagaagaac gggcaaaata
     1141 tggaatcact ggtatgcttc caagtactgt ccaaacactg gaacaacaat ctgttcaagc
     1201 ttatggtcaa tacttaagca aacaaacaga tttggaaaaa cgtatcttct taatgaatct
     1261 attcaatacg aatcggacat tgttttataa attgatgggc gaacatttag ttgaattcat
     1321 gcctgtcgtt tatgatcctg tcgttgcgga ttcaatcgaa caatacaacg aaattttctt
     1381 gaaaccgcaa gatgctgcat ttctttcaat cgacgaacca gaaaacatca aagcaagttt
     1441 gaagaacgcg gcagatggac gagacattcg tttgattgtt gtgactgatg cagaaggaat
     1501 cttaggtatg ggcgactggg gtgtgaacgg tgttgacatc gctatcggca aattgatggt
     1561 ctatactgct gctgctggta ttaatccagc acaagttttg cctgtatcga tcgacgctgg
     1621 tactaataat gaagaattat tgaataatcc cttatactta ggcaatcgtc atgctcgtgt
     1681 agaaggcgaa acttactatg aattcatcga tcaattcgtt caatcagcta ccgaattatt
     1741 cccagaattg ttattgcatt gggaagattt tggacgagga aatgctgcag cgatcttaga
     1801 aaaatacgaa gatcaaatca ctactttcaa cgacgatatc caaggtacag gaattgttgt
     1861 cctagcaggt gtattaggcg gattgaatat atccggtgaa caattgaaag atcaaataat
     1921 tttgacattt ggtgctggta ctgctggtgt gggtatcgca aacatccttt tagatgagat
     1981 gatccgtcaa ggtgtacctg aagaagaagc acgccaacat ttctatcaag tagataaaca
     2041 aggtttattg tttgaagata cagaaggatt gactcctggt caaatcccat tcgctcgcaa
     2101 acgctcagat ttcactaatg ctgatgaatt gacaaactta gaagcagttg taaaagcgat
     2161 ccatccgaca atcatgattg gtacttctac ccaacctgga gcattcactg aagaaatcgt
     2221 gaaagaaatg gctgctcata caccacgtcc gatcatcatg cctttgtcta atccaacaaa
     2281 attggcagaa gcaaaagcaa aagatttaat agaatggacg gacggaaaag cattagttgg
     2341 aacaggtatc cctgctgctg atgttgaata taatggtgta acttaccaaa tcggtcaagc
     2401 aaataatgct ttgatgtacc caggtttagg acttggtttg attgcttcaa cagctacacg
     2461 agtaaatgct gaaatcattt ctcaagctag tcgagcatta ggcggaatcg ttgatgtaac
     2521 aaaaccagga gcagctatct tgcctccagt agcaaaaatc actgaatttt ctcaaacgat
     2581 tgctgaaact gtcgcaaaat cagttgtcgc acaaaacttg aatcgtgaag aaatcacgga
     2641 tatcaaagaa gcagttgaat ctgctaaatg ggttcctgaa tataaatctt tagaagatta
     2701 agatcgttat agtaaaagaa catacagaaa ctttgtatga cgtctaaaaa gaaaaaagcg
     2761 actgcggcaa aacagcggcc agccaactcc tgctcttttg tcgcttttct ttggcttttt
     2821 tcatatgata cagaataaga aaggatctgt aaaaatggca caaaaaacaa caactgcaac
     2881 tgcagttgag gaaaagaaaa aagaaggttt ttggtccaca aaaatcaccg gcgtaagttt
     2941 gccactttat ttgatcatgg tccttgtcct gatcattgca atggctttag ggaaattacc
     3001 gacgaatatg attggaccaa tcgcagtttt ggttattttc ggtaatttat ttcattttat
     3061 cgggacaaaa atccctatcg tcaaaagtta tttgggtggt ggttccgttt ttgcgatctt
     3121 cgcttctgct gcgattgcta catttgggat cttacctgaa tatgtagtga aatccacaga
     3181 aaactttgtc agcaatatgg gcttttggga cttttatatt gctgccttga ttactggtag
     3241 catccttggt atgaaccgta atctattgat gaaagcttct gttcgtttta ttcccgttgc
     3301 acttatttca atggtcgtct ccttttttgc cgtaggactc gtgggcatgc ttatcggaaa
     3361 tggttttgca aattccgttt tatacatctc attacctgcg atggctggtg gtgttggagc
     3421 cggagccgtt cctttatcaa cgatttatgc aaatgtttta ggtacggatg cttcttctat
     3481 catttcccgc ctgattcctg cgacagcaat gacaaatgtc ttagctatca tcggtgcagc
     3541 attagttgct cgtgctggac aatcaatgcc aaaattcaac ggcaatggta aattaatgga
     3601 aaaaggcgat cttggcgatg gaaaacctcg aactgtgaaa ccttccattc cacagttagg
     3661 tgttggtttg atggtatcat tgactttctt catcttagga caaattggta actattttgt
     3721 tcctaaagtc catgcttacg catttatgat tattatcgtt gtcatctgta aaatcgccaa
     3781 cctattacca gagtattatg aagatgcagc tatcatgttc aataacttga ttgtcaaaaa
     3841 cttgacagca gctgttcttg ccggtatcgg tattgcgttg ctgaatctga atgtattagc
     3901 ttctgctttg acatggcaat ttgttgtctt atgtctgaca agtgttatcg cgatttcagt
     3961 tgtttcagga tttgtcggtc gtttgtttgg tttatatcca attgaatcaa ctattactgc
     4021 tggcttatgc aacaactcga tgggcggaac aggaaatgta gccgtcttat ctgcagctaa
     4081 tcggatggaa cttattgctt ttgctcaaat gggcaatcga cttggtggtg ccatcgtttt
     4141 gatcatctct ggtttcttga tgcaattact ttcttagtca aaaaaacaaa aatacctgat
     4201 catcaagtat cggcttggtg atcgggtatt tttttgataa agatttacag gattttcaaa
     4261 aatctttttt gcattttatc ggaatgataa ccgatgtgtt cataaaattg gtgtgcttct
     4321 actctgtatt ctgcagaatt aagccttatt gtcgttattc ctcgtttttt cgcttcttct
     4381 tctacgccag tcatcaaagc ctttccgatg cctctttttt cagcatattt agacacagca
     4441 agtgccataa tattgaacat tggatcgcta aaaatcgatt cataggactc cgcttgtaca
     4501 tagccagctg cttttttaga agtaggatct tcataaacca agaaaaaatt tttctctggc
     4561 tccttcagca gtttcttcat ttgtctcacc gtaacggtta atggtatatc ataccctaat
     4621 tgatccgcat tgatttcacg tagtgcttct gcatcttcta aacgcatttc ccgtaacatc
     4681 caaatcacca gccaatttct gattttttaa aatgaatttt tttatcatag tatactcttt
     4741 ttttcattta gaacaatatg tttcagtgat tttgacttgc gttagtctag attctcctaa
     4801 tgtttgtaag atgacaaagg cattgtaaca attgaccaaa agaaccacga ggacaattac
     4861 ttgaaaaata aaggtcactt tttttggaga caaaaccctg ctaaatcttg ctccccacaa
     4921 acctccaata acagcagcgg ggataacata aaacaacata gataaatcaa agcgcataaa
     4981 gctacttgtt aaagcaatcg ttaccaattt tgccagttga gaaaagaaaa tcgtagatag
     5041 agaatatagt gttgcttctt ttattggcaa tgcaaacatc agcatcaaca aagaaacatt
     5101 gactggtcca ccaccaatac ctaaaaaact agctaaaaag cccagaaccc ccccacatat
     5161 aaggtaccaa gtccatgacg tcaaatgaaa ttttggctga tgatgcttgg tgtagaagaa
     5221 cgcaaaaacc aaggtcaata ccgtcaaaaa aatttggatc agctgcacat gcttctcgtt
     5281 ttcaaataac tgtaaaaaaa cttcgaatac aatgttgccg actactcctc caacaacagc
     5341 tccactagat acccaaccga caatctgcca attcagtgat ttcccgcttg atacttgtcg
     5401 cacagtcgaa acaatggaca tcgtgaatac cgcaactgtt gagtaaaaag aaatccctgc
     5461 aacagaatgg gcgccgataa gatcaagaat cggtttgatc aacacgcctc cacccatccc
     5521 ggaaatagcg ccaactgtgt tcgccagaac gatcaccaca aaataaatca gaccaatcat
     5581 cttgtatttc ctttctcttg tttatatctt ttagcgattg ttactctttt cttttattat
     5641 acagtaaaag aaacaaacga ggcttcgaaa gcttttctac tttttaagga aatttagtgc
     5701 taaacgaaaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     5761 aaatagctcc tcataatttt cgtttggttg aacttattac cgcagatgca agcttttgaa
     5821 caccgtttaa gaaagaggtc gtgagcctgc cgttcagctc cgagtcacaa ggaacaattt
     5881 caaaaaacag cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga
     5941 gaacaccgtt taaggaagag actgtgaagt caacgttttg acctgattat tagagaaaca
     6001 aatggcaaaa tagttcttca tatttcgccg tttttcgagc gaatgccgag gggcagctga
     6061 ttgaacatcg tttatttgga aaaagggatt gtgacaagac gtttgtcaca atcccttttt
     6121 ttatatctta ttaattcatt cttttgttct attcttcttc ttccgccatg aatgagaaac
     6181 ccatatcaaa gaactataaa ggttatcaaa acagcgttta tgactaaatg aattagagtt
     6241 atctatcact atttttcaag cgatttaaac caaatggcat acaatatggc atacaaaaac
     6301 ttcaataagc cgtgggggtt tttaaatttc ttcttgttct ttaaaatcaa tgagtggagc
     6361 tacataattt gaatccactt tttcaaacgt cactttttgc atcgacgaat gttttctttc
     6421 ccaagataac gtaaatagat ttgactctgg acttataaag tctacttcac cttctagtat
     6481 tactttctta tgcaaatatt cctctaataa atatagactt gctaaagcat acaacacatt
     6541 ttccaaattt gcttccttat aatttgaaga acgaccatgt tttactctgt tatatatttt
     6601 ccaccaatct ggtgtatctt caggccaccc atctccaaaa ggttttagtg aagtcaatgt
     6661 taaagaattc ataaccctaa caccattatc caaacacatt aattcgctag atttaataac
     6721 agctttaaaa tcagttatgc ttttattgct actgctatca aatccgcaaa gttctttgaa
     6781 aataatctca cactccgaac caatcgctag tagtagtttt tgatattcaa tagagaatgt
     6841 tttgaaatta ttctcatcga gcatcacaaa atctgtggta ttaataaact cattttctaa
     6901 atgtagataa tacttccaat aaaaatttgt aaactctgct ttgtccatta aatcacctct
     6961 tttgaattat aacaaaaaaa acacgtccac aacagacgtg ttctttgatt tctacacaat
     7021 cagcttgcca aaaccatcca atcagtccta gcatgcctgt attaaaattg aacgttcatc
     7081 atctatcatc tttcttagtc cagcatgttt gcaaccacac ctacaatttt cagtccaaca
     7141 atagatttcg actagctaag tcttgcaaaa ttcaggcagc taacccttca tttactacct
     7201 taattatact aaatacccac aacgtttaca agtgtaatca ttctaagaaa tctgtttagc
     7261 tatgatcgtt gtataatctt taaacgccgt tccttcattg attgaaataa ttttataact
     7321 cttgtttttc caagctactt tcatatcgtt tgttagttca taatcttgat cgtatcgaat
     7381 aataaacgtc aacgttcctt caagaatggt acctacacta gcaagcacgt cttttagcat
     7441 ttgtgaacgc acattagccc aacattcaaa tacagtttct tcactatcca ccatctgccc
     7501 atcttcatct tttacccgtt ttgtcttttt aaacttgact ctctcagtca attcatttac
     7561 attcttaatg attggcataa taagcacctc tcatttgttg tactaatgct tgtaccgtta
     7621 ctggtatccg ttcgctgctg gcttcttgac ggttcaaata ccaatgttga gtaagtaatg
     7681 acaccgccaa atcaaattgc tgataattat ccatatcttt atccgttgcg ctactatcaa
     7741 tagcactttt tatatattcg cctgctgtat caatcaattg ttgaataaag tcatcatcaa
     7801 tagtgtgatc gatccgcata ctttttttta tgctatccaa aattaccatt ttatctctcc
     7861 taatgaaaaa aaggcagtgg gataaaccgc cgcccaatat ttttctatac ttttggtgtt
     7921 acaactggcg taaactcaat atacactgaa gcattttgat caattttctt ataatcgttg
     7981 cgaacgatca ctgcaagtcc ttgtgagtag taatcgaatt tttcccattg agtcgttact
     8041 tgatttctac gagcaacaaa gatagattgt gcaatatccc ccataatcat agggaatgtt
     8101 ccagctttag gattggcaaa caatgtatct gcaatcaata ctactggcat accgaataat
     8161 gatttaccac taggtgctgt cacatcgggt tgtaaaatgt aacgtccatc tgaatctttc
     8221 aatgtatcta ggtggtaata tccgctttgg tttagaatta ccattttatt taatgctggg
     8281 tctaatgcca cattgtatag ttgtttcaaa tcgtccaacg tagcagccgt tttcttagtg
     8341 aatgttttta ataaatccat gatatgctta ttgtcagtgt tttctaccaa tttcgctaac
     8401 tgatctttga cctcttgtac aatattcact gctgaatctt ccacaacctc atttgataag
     8461 gcaatcttac cagcacgagt ttctacttta taatcaactg aagtaaacat ttcagcgtca
     8521 atatcaccaa tttcagcaag ttcagctttt gttgctaaca ctgcttgttg gttagttgct
     8581 actggatatt taccttgtcc atttgatact gtttttactg ttgcatattg agctaagtta
     8641 taatttgaac gtttcaaatc aaagacttca ccgattactt cttcgggaac aactgccgct
     8701 gcatttacag tagtgactcc atcacgcact tcgccttgtg aacggatata ttcttcatac
     8761 ccacgcaatt ctgttttttc gttatcgatt aaagtttttt ccatgttatt actctccttt
     8821 tgtgctttag aagtggattc taagctattg tttgtttgtt ttgcttggtt gttactcata
     8881 aacgattcgt aagaacgctt atttacttgg acatttgatg aatcataagc gggaacggtc
     8941 acgacactaa tttcattaag tgctttgatt ttatctattg atcgttcaat tgtgccatct
     9001 tcttttttgt cgaatgaatc taatccaagc acgaacccaa aggacataga atcaacaacc
     9061 ccttttgaga tattctcata cacatcattt gaataggtcg tgtcggttag ctctgcttca
     9121 aaatgtaatc ctgtatcatc gacattcaat ttcaacgtac ctgctttgac actagctagt
     9181 ggctttgaat aatcgtgtcc atataataag aacacatttg ataaatccac ctcttttaat
     9241 gcttcgggtg taatcacttc tacaaagccg cctaaatctt ttgacggttg cccgaatttc
     9301 aacgcatagc ctgaaatagt tttgccgtct gttggttgtt tttcattttc tttctctact
     9361 ttgggagaat cggctgttag ctcagcttct tctgtcagcc gtttttcctt ttcttcttca
     9421 ttattcaact ggtgtaactc cttcctgttc ttttaattga tatttctcca acgtatctaa
     9481 atacgtaaag ttcaaacttg ctagtaaacg atcgccacca tccatggggg gtagccccat
     9541 tttcgaccgc ccctcattaa tcgtgagaag tgaccctttg acctgatcca atacattttt
     9601 tacttttgtt tcaggatcag tctctagtaa ctgatcggaa ttaaagcgga tattggtttc
     9661 aagtttcgta tctaattcac tcacaaacac attaaagtaa tgaatcagcg tactttggat
     9721 atactggaga ttactttgaa cggtgctaga atgttcattt tcaacgccta aacgctcaat
     9781 cggtacacca aacgccttgg caatttgttt tgtgttccaa tcattggaat tgataagctt
     9841 caaaacatcg gtgtttattt ctaatgtttt gtagtccatt gtttcatcaa gaatgatcgt
     9901 tctaagagca ttatctccac tacttgaacc gttggcttct tcaaactttt cacgaattgc
     9961 gttctttgct gatccgtcta aatctgactt atgaaccttc aaaatacctg acccactgac
    10021 accacgagta aagaaattgt gcaatgtgcg gttgccagct tgttgaattt tcagttcatc
    10081 acgtaacgca taaagtggtg gtaatcctgt taaaccatct tgggtgaaat atttgaagtg
    10141 caaaatatcg ctagacttca cacgtctttt tttatcacca atctgataag acaacgtacc
    10201 attatctaat tgagtaaccg ttacacttga gttaggtaat aagtgaattt cttctacctt
    10261 ttcaccgttg cgtttaatct ctgcaaagct attaccgttt agcaacatat tgaccgccaa
    10321 agcaaacttt aaatgccaac catccatttc tgaattaggt ttctcgttta gtaacttcac
    10381 cagttcatta tcagcttgtg gcatattgtt tttgaccaat tgaatcggac ttgaagcaat
    10441 atcactagca ataattcgaa cggctgcaaa cacatcacta tttcttaatg cacgaacgct
    10501 tgtaaagctg gtggtgtaat catccgattg gattgatacc acatgttgta ggaaaggatc
    10561 tcccgttgta ttttgtctcg ctttaaaaaa tgccatcttt taacctccct tctgttctgt
    10621 atttagcatg accgctaccc caataagcac tatgcccagc gctaacaagc catagtagac
    10681 atttgttagt aaggtgatag caaccaacac acacaccaat ccaagaagta aaagaacggt
    10741 atgaatgtaa ttagaaagaa aattcttcac ttgtataaaa ttcattgtct gctttcgctc
    10801 cttccgttgt ggtgaagtaa tccattgcaa acacataggc attgattaat gcagctattg
    10861 gatcaatttt gttactgttc tttgatttat taatctgaat gccgttgtta tccgttttta
    10921 aaatagcgtt gttcactgca tgggtgagaa tcgtattttt attgtgaata acgttgcctt
    10981 cataaacttg ttctctaaaa gttcgggtcg gaacgtttag agtaatcgtt ccttgtctta
    11041 cttctaacat tgggtagttg gctttttcag ctttactgat aagcgaatta gcgttatacg
    11101 ggtcataaca aatccccata cattccaaat cattttcttg aatcaaatct tgaataaact
    11161 gaaatacttg gtcatagtcc acgattccac tttctaattg agtaatggaa cactcaccag
    11221 ctttttctag ttctcgataa ggcaaaccgt cgcgcttttc tttgtcttga agcccatatt
    11281 tggtggctac aaagctatgt gaatcacaat aaagttcgcc gttatctagc ggtacgatcc
    11341 atgaaacgct agttaaatca tctgttttag ataaatccac tccgatataa acgggcttgc
    11401 ctgtaatatc ttgtggttct acttcggttg cctgccagtc ttcagcaatc atatagctgt
    11461 cctcactcgc ttgtctccat aaattgaagt ttttcaccaa tacagaatta aggttatttt
    11521 gtttcaaagc aaggttcaca tcatcttgaa tggttggaat cattactttt ttgatttctt
    11581 cactttcaaa aatcggattg gctttgatcc atttctcttg atcgtgaatt tcttcgggat
    11641 cgtccaattc ccatatcgct ataaagtacc tgtcggcttg ttcttcgcct tttagcacac
    11701 gatctaacaa taagtattcc tcgtacatag gaacgtttaa atcaagccca gcggtactaa
    11761 taacacacaa taagccgttc ttttgttggg tcattcctga cttaatgacg ttgtacgttt
    11821 tacgagtttt tgcttcgtgc cactcgtcaa gaattgcggt tgttggtgca aacccatcta
    11881 gcgtgctggt ttcactagcc agtgccatag cgaacgaatt agagggcaag tgctgaatct
    11941 tagagttcat aattttcagt tgtggtctta aaaacttact agacttgacc acgtttctaa
    12001 gtgaattaga cagcatatca tagcccaatt tagcttgttt caaagcgtta cttacaaaca
    12061 atacttgcct tgcttctgct ggttcttgtt ccataatcaa cgcattggca gccatgccac
    12121 ttgcgatata cgttttccca ttcttacggc tcatactggt aaacgctcga ttgaatcgcc
    12181 tgtaattccc ttctttagta cgccagccat aaagactgcc tagaatgaat ttttgaaatc
    12241 ctaacatctt gattgcttta ccgtccgtag atggtagcaa ctcaatgaat ttaatcgctt
    12301 tggtggcttg ttcttcatcg aacacataag ggaagctatc tgacttagaa cgctccaaat
    12361 cgcttatatg acgctcacac gcttggataa ttttcttggg tgcaatcagc ttacctgata
    12421 ggactttctc aatgtaatca ttcatgaatt catcaactct ttgaacgggt cttcgggttc
    12481 atcattttta accttgttca aagctagttt tgatcgtgat tctaaggtca tacctagtga
    12541 ggtggcttgt tttttcaaat cactcatagc ttgtgattga acccgaaccg ctggattggc
    12601 tttctttact ccgttttcgc atgtgataaa cgtacctgat tttcttatct cggcttcggc
    12661 tgttttcatt cgagcaaagg ctaaacaata ggctaccaat gaaccataat ctgcttcact
    12721 caatggataa tcttttttta atgttggtac gatacgattc cattcacttc tagcgctcgc
    12781 tgccatccag tcagggggcg tgatatttat taatggttgc tgcttgaaca attcttctct
    12841 agcgtccaca cggattgctt tttcttcact tgatatattg ccttttgtat cgtgtaatat
    12901 tttagggtgt cccatgttgt cctcctttca gacaaaagaa aaaggaacgt aagcaattaa
    12961 gctcacattc cttcgttttt cgatcagaat ttttctttat ctaatttatc tatataaatg
    13021 atttttccgt tctgcatttg aatggtcact tgaccaaaat gaggtaactc aatggtgcgc
    13081 tgtttccctg ttttatcagt aataacaatt gtatttttca ataaatctcc tactttcatt
    13141 atatactttt tcacttgtga ttacaagtgt aatcgttatt tgtaattacc cttatgcctt
    13201 ttcacaacgg ttatttgtac caaaatgagt aggcatcgat tgtcagtaag tttcacacta
    13261 tgccccctta actcaaaggg tactcttttc tcgtctttgt ctttcattgg ctgtcttgga
    13321 attatggtgt gcatggcata aagaacgaaa attagtttca tctaaccgtc tattccaatc
    13381 atcttttagt tcaattttgt gatctacaac atcagctttc cttatcaagc cttcttctag
    13441 gcaatcctca catactggat gatttaaccg atacaagtat gaagcctttc gccatgttct
    13501 actttgatag aattgaaaat atttaccttc gctttgcttt ctttcttcac gcttaattgc
    13561 cgtttgcttt gctttatgct tcccacaata cttctgccta taatcaatca atgttttaca
    13621 tccagcatga ttacattgtt ttttaactgc catagattac caaccaaagc gatctcgttc
    13681 gtcagaatca tcttgttccc attttgattt gtattcaata taaggcgaaa ctatcttcaa
    13741 gcacgttccg tctttaaaat taactttagt gattttatct tcctcaaaac taataaagtc
    13801 tacatcttct tgagtgtttg aatactcaat gtgtttctca ccatcgaagt atctaatact
    13861 ttccatatgc ataattattc tacctccaat aaattagtta tcaattcatc atctgttagt
    13921 agcaaagcca agaacttatc atggatgttt aaaaacaaat tatcctcatt cgatgtaagc
    13981 tctcgttgtc tcgctcgatc atataggtta agcagacaat taaattcatc cattagctca
    14041 cctgtatcat catagttgcc actatctacc aatgatccca ctactagcga ttgacaccac
    14101 gctactgact cctgcagcca atcaggttct tcttccatta tctttaatac ttctggatca
    14161 aatgtggaat aatcttctgt taccaatccc acactaaatg ccagcgtgtt cattagaaac
    14221 cgatccattt catctgtata ttgaaagttc ggtgctttgt ttgttgaaat aatattgagc
    14281 atttactttc ctccttgatt cgtaacacgt ggtaacacct acgtaacagg tacccgttac
    14341 cactcaatcc caacggggac aagggttcta gcgttttggt aacaggtaac gggtaaaatc
    14401 cagtaacatt attccccagc atattagcta actatttcaa aagttttaag gaaataaggt
    14461 gttaccccgt taccaaattg ctgtatccct tggggcagta agggtaaact agtaacgggc
    14521 taggtgttac tgacccgtta ctacccgtta ctttttgtac tttccagctc gatttttatt
    14581 ttcataggca tttgacttat tgtaaaatcc tcgtcgttta tttctttttg gcagttgctt
    14641 ccgttcccaa ccaggaatac tgctaataat caaggcaatc tttttagttt ctgcatctga
    14701 tcgaccattc aataaatcct ttgcttgttt atcaaaaaca gcttccaaaa tatcagcggt
    14761 gagtacgcta tcaactaaat aaaactcttg ttgcatagta gctccattat agaaatgctg
    14821 taaatttttc tcattacatt tactgtcagg atagttctga taataagaac gtctcgccca
    14881 cgctggagct tcttcccatt tatacgggat ttccatttcc aaatatttag tgattgcttc
    14941 tttaattgga ttctccatct tggcatcctc ttgatagtct tttgcaattt ctaagacttc
    15001 tttatcttca tcgggactaa aatagattcg ttgtccttta tcaaaaagca cctttgcttc
    15061 agctaacact tgaagaaaat attcatcctt tgttttaaac ggattcatct ttggcttatt
    15121 cttacaaggc aaaggataaa aacggcggtt tcctgtatca tctttcaaat actgcccatc
    15181 attcgacgtt ccaataaata cacattgcct tgcccaagct atgacattcc gaccataagg
    15241 aggtctaatt ttatcgatct tggcactgat gaagtttttt atctgttcaa ttttagtttt
    15301 gttcattgaa gataattctc caagttcaat aattacattg ccttgaagct gcatataatc
    15361 atctttcttt tcacctagcg aatctaatga atcggtgaaa aaatcatccg tgtaaagtaa
    15421 tgctggaacg gtactcttac caatcccttg cggtccatcc aaaactggaa cgatctcaaa
    15481 ctttattcct ggtttataga tacgtgcgac tgatccgact atccaacgtt ttgttacttc
    15541 tcgtgtgtaa aggttgtcgt ctacccctaa aaggtcaata aacagtgttt ccagtctagg
    15601 ctgttgatcc catttaactt tttcgatacg ttcttttact gggttgaaaa cattctttct
    15661 agccacattt actactgctt tgtgtatttt gttgtcatca aaaactacat tgtagttttc
    15721 ttcaatatat gccgttaagg catcctcaaa actgtcttcc cattctccag cggtacgatt
    15781 gatccaagga gaatctttta ataggtgaat gtatccgcta aattcgttat aagcaattgt
    15841 tccagctaaa ttgctatcat tggttaaaat caattctgca ttcacaacac tgttactttt
    15901 aatctgatcg tctttattgc atcttaatcg tgacatccag gttttcaatt caatgatatt
    15961 gtcttcacgc tcgctggctt cgggaaagtc taaaatacta tctaatttat gcttatgagc
    16021 catgagcttt ccccctcaaa tcttttttaa tcatcgatcg aataatttga ttcacttctt
    16081 tatcttttag cggtggttca ttgtacgcat ttgctgtata aatcaattct gctaaagttt
    16141 gattatctac accgtgccat agtaaccaac cgcataactt agttaaatga tcgtttctac
    16201 taccttcgcc agcgccttga acaatcatat ctaatttttt tgctgtccct gtttttgcta
    16261 cgggtcggta ttgagtcttt atttcttgcg gtttttttag cagtaggttc actagccaat
    16321 cgggagctgg tgcaatatta tttagagata cgttttcaat aggttcatat gctctcccaa
    16381 cacttcttga aataatcata gttggcgcta ttggaactcc tttagtgata atatcaatcc
    16441 cgcttaaact tttaccttta tcatcacgcc cgaagatatc agcaaatgca cctgtttctt
    16501 gttggataat tacaccatca ggaattttaa agaagaaatg gattccttta cctggtgtga
    16561 tttccgtata agtagaagga aatggatcat acattgaagc aagtttttta taattctcaa
    16621 cgccgtttcg tgtttcagaa tgttgatcta ggtcaactac caccatttga tgatcttcaa
    16681 gcattaaccc aatatttcta gttgcatctt ccgtccacca ttcattaatt tgattagaat
    16741 cgttagttgc atcattccaa ccactagagc cttttattgg aattttagtt ccaggagata
    16801 agggataaat tgaccatccc gacttttgta attccgttgc tgcttgataa ttatttgcca
    16861 ataaacctct cctccttttt caaaatactg gtataaatca ttcagacacc tccatgagct
    16921 tcaactgctc aagttcgtat ttcaacccat cccgaacaaa ttcagcacct tttaattgat
    16981 tctgatactt tacccatgta ttaaatgttt tcacccttgc ttcttgcata tcagtaatgt
    17041 ttaaccatgc ggttaaaatc tgatttttta gctcgttgat ttctcgacaa attattttgt
    17101 gttctgcatg ttttttgagt acatacaaaa ctgttagttt ttcattgaat gaatttccaa
    17161 agtaggtttt cccgatatta ttttctagtt cagcgatccc ttgattaatt tcctctaatt
    17221 caaggctatc gagttggttt aatagttttg ttttgtacat ctacttatgc cctccagttc
    17281 ttaattgata gtcgtactcg tcataagcag tgaagagata taacgctaat cctgcaaaga
    17341 tagctatttt tgcaattaat ggtaataagc cgaaaattcc gccgattaaa atgcttccta
    17401 taattaagtt tcgtttcata atggttccct ccgatttttt aatatggtat aatcggaaca
    17461 aagaatatcg ggttttatcc caattaagtt gcctttatcc tgtccgccaa gactgttatg
    17521 gataaggctt tttctttgtt ctcgatcatg ttaatgctcc taagttgtct gcaattcagc
    17581 agaacgatta taaattttct cactcactga aaactgcaac ccatattttt ctttaacagt
    17641 cattaattct actgtctcat ctaaaatagg ttcacgatca accaacattt caggagtcat
    17701 atctttcttt tttaccattt tggaatgacc ataaatagtt gatacagctt tattggctat
    17761 cacattcgct ttaatgtagt ctactggttt tggttcagta atagcgtggc ttaatttagt
    17821 cattgcttct ttttggtgtt ctttgtctag catgcggaat acttgaaagc cttcaagtcc
    17881 tgtggactgg cgtagttctt tgatgatgtc gaaaatccag tcttgaaatg cctcggcttc
    17941 ttttttacgg cttttgaatg ccatgcggta aatatttttt ttcgttgatg atgtttacat
    18001 cttgtgttct gcccaatgaa tcgatgacct tactagtagt aaccccatct ttaggcaaag
    18061 agtgaatggc tctagtaacc tgtttaagac ctaacgcttc cgcaacatct ttcgccactg
    18121 cccaccattc atcattaata tcgacaaatc ttataatgtg tccgttccaa atttcagttt
    18181 tcattcaacg tcctccagtt caagccctag caagctcgct aacaactcaa cttgctgata
    18241 caaaatttct tgatactctt caatagaaag tcgttcttta aactctgctt gtactaaatc
    18301 gttcaagcca cgcaatactc cgcttaccgt tggttcttgt ggtagcaatt tttgatagtc
    18361 catttgttcg tacaaacatt caaacgtttc ttctagcgct ttgccttcca agtttgctaa
    18421 ttctgccgct gtgtcttctg taagaattgt tgataatact ttttcgattg ctttattttg
    18481 tgtcgattcc attattattt cctcctatta atccctttgc ccccacggct tgcgtttatt
    18541 tttggtattc ttccataaag cgatcacatt catcttgtga aattcttacg actccatcaa
    18601 ctataatcaa ctttaaaccg ttaggaacat atttctgcat catagtattg tatgaaacgt
    18661 tcatatactt agcagcttgt gccttgttca tatattttgg aaactggtac tttttttcta
    18721 ataatgaaag taacgttcct tgagtttgct ctacttgttg aacaactcga tcgtcaataa
    18781 ttttaaaagc tggatcatca agcgtgagtt tgtcaatttt taattccata tttatccccc
    18841 ctcgttagca tacatttcta tacaaagatt ttaaaaaaat gcaagaggct cttgaataca
    18901 tttctatacc tcttataaac tcaatatacc atcatacctt ttcatttgca agaattttaa
    18961 catacttttt tgaactttta tgtttcacac atatttaaat cgtgatatag tagagaaaat
    19021 atatgaatgg tggtgaaaat gtggctagaa aaaaataccc cgaagcagtg gagacaggac
    19081 aaagaattag actggctaga attgaaaagg gactctcaat ggaacaactt ggaggtaaat
    19141 tatccccacc ggccagtaaa ggtgccgtta gtaattggga aaatggctat aatcttccca
    19201 ataacaccag gcttaaacaa ctttcaaagg tattagatgt atccactacc tatctattaa
    19261 acggatctta tacattaaga gatatgcata tgatgcccga aattaagcga aatgtgcttg
    19321 aaaagcaatt gaaaaaaact attgaaaaaa ataactctat aattgattct aatctttcta
    19381 atgctatcaa taacatcgaa gaagataacc tgactgtctc acagaagatt tatttaacaa
    19441 acgcaataaa atatcttaaa acaggatctc gaattgaatt tcctgacggg agtaccccaa
    19501 ttgcttatct cgcagctctt ctaaatacgc ttttacgata tgactcatta attaatgatt
    19561 cagaaaaaaa tacagatgag aaaaaagagt atcaagaatt ttatgaaaat gatcttctaa
    19621 aagagttcac agctcttgtt actgagctaa ataaacacta ttcaaaataa taataaactg
    19681 cctcctccaa ccgcccccac ggcttgctac actatggagg gaaaactatt atggcaacat
    19741 tcaagcaata tgaaactaaa aaaggtaaat tttggctcta tgaagcctat cttggaatga
    19801 acaaaatgac tggcaaacct gataaggttc gtagacgagg ttacaaaaca aaaaaagaag
    19861 cccagcttgc cttgtcacgc ttgcaagtgg actacgataa aaacggtatt aaaaaagcga
    19921 acaacgaaac gtttaaagaa gtttatgact tgtggctaga aacatataaa accactgtcc
    19981 gagaagccac cttcatgaaa actgaaatca agtttagaaa gtggattctt ccaaaatatg
    20041 gaaatcttcg tgtgaatgaa gtcacggtaa aaaaagccca acaaattgta aatagctggg
    20101 ctgaaactac tgaccaatac aaggtacttc attcaacttc taagcgtatt tttagctacg
    20161 ctatcaatct tgggattatt gagactaatc cactagacaa cgtattaatg cctaaaagaa
    20221 ccaccactaa gcataaagaa aaggtcaaag tttacagtaa agaacaactg atcaccttgt
    20281 ttgattacct aaacagcaaa cctgaaactt atcgaaacga ctttgataaa acgttactac
    20341 gctttctctt ttatagcggg gttaggatca gtgaagccct tgcattgaat tggtcagaca
    20401 ttgattttca agaaaatacg gtcaccatca ataaaacact aagccaatcc aaaaatggct
    20461 ataaaatcag tgatcctaaa actgattgta gtgctggggt tcttccacta gatgaaagaa
    20521 caatttctat attaaaaaaa tggcagatta atcagcggaa atatatgctg acacttggtg
    20581 taactgatcc gactatgatt ttttgtggta tctataaaga actaatcaca caccacgcta
    20641 tttatgcaag attgattact atttgtgaaa aagctatggt tccatttctc ggtgtgcatg
    20701 tagcaagaca cactcatgct tcaatgctct tggacgctgg agctactatg gtagaagttc
    20761 aaactagatt acggcataca aaaattagtc agacgatcga ttgttacggt cacttggcaa
    20821 aagaaacgaa agaaaagact gttgaaaaat tagtacaaca cttaaatatt gactagatgg
    20881 catacaatat ggcatacagt aaaaaataaa aaaaggagaa accctatcat atcaaggttt
    20941 ctcctagctt tatagtctat tcttcttctt ccgccatgaa tgtattgaat acttcttcaa
    21001 tcatgtccca ttctgcgtct gtttcgattt gttctaactc gccttctgtt ccatcttcgt
    21061 tttcgatgta tgcataagct tgtaattcta catcttcatc ttctgaaaca ccagcaggat
    21121 ataaaagaac atagttacgt ccaaattctt cttgtccgtc aactgttagt aggatttcat
    21181 ataatgtttc atttccttgg tcatccacca atgtgatgtg ttcgtgtcct tcatggtcgt
    21241 gggaatggtt atgttctgtc ataagattac ctcttttctt ttattattaa aataattgct
    21301 tacctgagcc gtctaaataa ttttgtaaga tcatcacagc tgctaattta tcgatgactt
    21361 tttttcgttt ggctcttgaa gtatttgcct gctctactag catacgttct gcctgaacag
    21421 tggttaatcg ttcatcttga taatcaactg gcaaggagaa tagttctttg atcttttctc
    21481 cgtatgacat ggaagcttct gcccttggtc cgatggaatt gttcatattt ttaggtaatc
    21541 caactacaaa ccggtctact tcatattgtg ctactaattc cttcaaccga tcaaagccaa
    21601 actcttcttc tgcttcattg atccgaatga tctctactcc ttgagctgtc cagccaaagg
    21661 ggtcactgat agcaacacct actgtcttag aaccgacatc caagcccatt actctcataa
    21721 attcactcca tgatttgaga ggtagtcctt gacaattact tccatgatct catcgcgttc
    21781 gtgacggcga atcaaatttc gagcatcacg ataacgagga atgtaggccg ggtctccaga
    21841 aagcaagtag ccgacaatct gattgattgg attgtagcct ttttcttcca aagcccgata
    21901 aacaatcgct aatgtttcgc tcacttcttt tttgcgacta tcgtcaaaac caaaacgtac
    21961 ggtttcatct gtaaaaccca tgattctaca cctctttccc gcaaggtaat tccagcttac
    22021 cttctacaac cttattttac tcgaagaaag ggaaaactac aaacaaatat tccaaacttc
    22081 cgaataaatt aagaaacact atttgtatgt ttttttcctt ttttctgtag ctttatcaaa
    22141 agtactcttt tcataaagta tacgctatat ttctttgaat gtatgttttt acacaatttt
    22201 ttcataatat tcataaaaaa ttcgaatagc tgctactaat tttttgtttt tgttgcttgt
    22261 cctgtctcat aattgatcgt gtagccatct tgaacattgt agataaagac attgaattca
    22321 atttttttgt cttcaatgct ttctgcttcc aattgcactc ccctggctac cagttcattc
    22381 ccctcaaaat gaggagtgac acggtagcgt acatgatgat ttgtttcttt gatatatgcc
    22441 gcaatatcat tttcgtgagc aagcatgtat ggagcattca acgaacgtgt ccctgtcatc
    22501 aagtttttaa tgttgttatt ttgaccagtc agctggaaac caattaaatg gctgcgatta
    22561 tataaccatt gcccgtcact taatttcttt tgtttccaac ctgtcgggtc aatatacaaa
    22621 ggctctctct tttcagtagg gaacaaatct tttccaagca tagcattggc ttgtcctact
    22681 ctgttcaatt tatcaatatt tgaatagctt tcccatgttc ctttggaaag gctaagatcc
    22741 tcttttgtaa attccggctg attcccattg atttctattt cttgatgttt tccatcatat
    22801 tctggcacat ccgctacttg agaagcttgt ggtgtggaat caaaaatagt tgtaatctgc
    22861 tgaaagatat ctgaatcttt ttgtgtggtg gaaacaccga atgcccctgc aatcacagca
    22921 gcgacgatcc ccacgatcgc cttggttgtt tttttgctca tttctttact cctttactaa
    22981 cagctcccta aatcttaaca tatgttcccc tataaataaa tactagaaag aaatagaaac
    23041 agcaaaaaat aaaacctgtt ttaagatggc ttaaaaatac aaaaaagaga cggattcctc
    23101 gtctctaatc agtcaccact ctatctgtaa ggtatttatt attattcaaa aattttcttc
    23161 atactcaaga caatacactg atttcacaac cgcttggtaa acggtgcgca aaagcttgca
    23221 tctgcggtaa taagtccatc caagttaaaa acataagaag ttgtttttgc ttggctgttc
    23281 ttattacttg ttgcagagca gctttttcac aacctatttt aagcgggtga cgggaatcga
    23341 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcaatacaaa
    23401 aacagtatac gcctctttta ttccttcctc aagttttttt attctttacg cttctggtat
    23461 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
    23521 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
    23581 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
    23641 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
    23701 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
    23761 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
    23821 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
    23881 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
    23941 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
    24001 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
    24061 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
    24121 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
    24181 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
    24241 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
    24301 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
    24361 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
    24421 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
    24481 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
    24541 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
    24601 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
    24661 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
    24721 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
    24781 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
    24841 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
    24901 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
    24961 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
    25021 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
    25081 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
    25141 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
    25201 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
    25261 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
    25321 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
    25381 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
    25441 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
    25501 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
    25561 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
    25621 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
    25681 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
    25741 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
    25801 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
    25861 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
    25921 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
    25981 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    26041 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    26101 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    26161 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    26221 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    26281 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    26341 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    26401 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    26461 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    26521 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    26581 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    26641 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    26701 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    26761 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    26821 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    26881 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    26941 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    27001 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    27061 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    27121 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    27181 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    27241 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    27301 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    27361 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    27421 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    27481 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    27541 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    27601 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    27661 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    27721 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    27781 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    27841 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    27901 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    27961 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    28021 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    28081 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    28141 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    28201 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    28261 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    28321 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    28381 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    28441 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    28501 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    28561 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    28621 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    28681 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    28741 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    28801 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    28861 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    28921 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    28981 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    29041 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    29101 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    29161 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    29221 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    29281 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    29341 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    29401 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    29461 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    29521 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    29581 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    29641 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    29701 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    29761 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    29821 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    29881 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    29941 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    30001 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    30061 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    30121 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    30181 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    30241 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    30301 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    30361 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    30421 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    30481 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    30541 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    30601 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    30661 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    30721 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    30781 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    30841 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    30901 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    30961 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    31021 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    31081 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    31141 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    31201 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    31261 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    31321 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    31381 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    31441 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    31501 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    31561 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    31621 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    31681 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    31741 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    31801 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    31861 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    31921 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    31981 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    32041 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    32101 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    32161 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    32221 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    32281 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    32341 aacaccctga cattttgatt ctagatgaac caacgaacca tttagatatc gagacacttt
    32401 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    32461 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    32521 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    32581 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    32641 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    32701 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    32761 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    32821 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    32881 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    32941 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    33001 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    33061 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    33121 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
    33181 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    33241 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    33301 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    33361 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    33421 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    33481 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    33541 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    33601 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    33661 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    33721 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    33781 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    33841 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    33901 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    33961 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    34021 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    34081 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    34141 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    34201 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    34261 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    34321 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    34381 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    34441 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    34501 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    34561 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    34621 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    34681 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    34741 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    34801 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    34861 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    34921 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    34981 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    35041 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    35101 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    35161 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    35221 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    35281 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    35341 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    35401 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    35461 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    35521 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    35581 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    35641 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    35701 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    35761 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    35821 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    35881 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    35941 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    36001 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    36061 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    36121 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    36181 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    36241 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    36301 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    36361 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    36421 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    36481 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    36541 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    36601 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    36661 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    36721 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    36781 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    36841 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    36901 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    36961 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    37021 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    37081 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    37141 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    37201 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    37261 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    37321 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    37381 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    37441 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    37501 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    37561 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    37621 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    37681 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    37741 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    37801 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    37861 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    37921 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    37981 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    38041 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    38101 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    38161 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    38221 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    38281 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    38341 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    38401 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    38461 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    38521 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    38581 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    38641 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    38701 ctggctgtag ctaaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    38761 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    38821 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    38881 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    38941 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    39001 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    39061 ttcaaaactt gttttatata aattgaccct acgaccaaag ctgaattttc tggtccattg
    39121 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    39181 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    39241 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    39301 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    39361 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    39421 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    39481 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    39541 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    39601 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    39661 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    39721 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    39781 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    39841 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    39901 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    39961 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    40021 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    40081 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    40141 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    40201 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    40261 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    40321 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    40381 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    40441 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    40501 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    40561 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    40621 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    40681 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    40741 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    40801 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    40861 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    40921 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    40981 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    41041 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    41101 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    41161 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    41221 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    41281 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    41341 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    41401 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    41461 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    41521 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    41581 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    41641 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    41701 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    41761 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    41821 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    41881 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    41941 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    42001 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    42061 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    42121 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    42181 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    42241 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    42301 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    42361 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    42421 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    42481 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    42541 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    42601 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    42661 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    42721 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    42781 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    42841 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    42901 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    42961 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    43021 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    43081 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    43141 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    43201 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    43261 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    43321 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    43381 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    43441 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    43501 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    43561 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    43621 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    43681 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    43741 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    43801 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    43861 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    43921 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    43981 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    44041 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    44101 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    44161 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    44221 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    44281 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
    44341 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
    44401 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
    44461 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
    44521 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
    44581 gtattaaata atttaatttt tttgttccta tttatttaac
//
LOCUS       DAZKSU010000015        42208 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000015 DAZKSU010000000
VERSION     DAZKSU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.015"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            247..1110
                     /locus_tag="ACG8Z3_000865"
     CDS             247..1110
                     /locus_tag="ACG8Z3_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0823203.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQAIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1239..2861)
                     /locus_tag="ACG8Z3_000866"
     CDS             complement(1239..2861)
                     /locus_tag="ACG8Z3_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0823204.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3110..3631
                     /locus_tag="ACG8Z3_000867"
     CDS             3110..3631
                     /locus_tag="ACG8Z3_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0823205.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3817..5547)
                     /gene="spxB"
                     /locus_tag="ACG8Z3_000868"
     CDS             complement(3817..5547)
                     /gene="spxB"
                     /locus_tag="ACG8Z3_000868"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0823206.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5712..6530
                     /locus_tag="ACG8Z3_000869"
     CDS             5712..6530
                     /locus_tag="ACG8Z3_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0823207.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6633..6830)
                     /locus_tag="ACG8Z3_000870"
     CDS             complement(6633..6830)
                     /locus_tag="ACG8Z3_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823208.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6999..7577
                     /locus_tag="ACG8Z3_000871"
     CDS             6999..7577
                     /locus_tag="ACG8Z3_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0823209.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7672..7809
                     /locus_tag="ACG8Z3_000872"
     CDS             7672..7809
                     /locus_tag="ACG8Z3_000872"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823210.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8623..9126)
                     /locus_tag="ACG8Z3_000873"
     CDS             complement(8623..9126)
                     /locus_tag="ACG8Z3_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0823211.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9485..10510
                     /locus_tag="ACG8Z3_000874"
     CDS             9485..10510
                     /locus_tag="ACG8Z3_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0823212.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10857..11735
                     /locus_tag="ACG8Z3_000875"
     CDS             10857..11735
                     /locus_tag="ACG8Z3_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0823213.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12211..12327
                     /locus_tag="ACG8Z3_000876"
     CDS             12211..12327
                     /locus_tag="ACG8Z3_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0823214.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13255..13593
                     /locus_tag="ACG8Z3_000877"
     CDS             13255..13593
                     /locus_tag="ACG8Z3_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823215.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13687..13917
                     /locus_tag="ACG8Z3_000878"
     CDS             13687..13917
                     /locus_tag="ACG8Z3_000878"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0823216.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14317..14688
                     /locus_tag="ACG8Z3_000879"
     CDS             14317..14688
                     /locus_tag="ACG8Z3_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0823217.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14962..15654)
                     /locus_tag="ACG8Z3_000880"
     CDS             complement(14962..15654)
                     /locus_tag="ACG8Z3_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0823218.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15657..16442)
                     /locus_tag="ACG8Z3_000881"
     CDS             complement(15657..16442)
                     /locus_tag="ACG8Z3_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0823219.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16679..18079
                     /gene="lacG"
                     /locus_tag="ACG8Z3_000882"
     CDS             16679..18079
                     /gene="lacG"
                     /locus_tag="ACG8Z3_000882"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0823220.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18104..18421
                     /locus_tag="ACG8Z3_000883"
     CDS             18104..18421
                     /locus_tag="ACG8Z3_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0823221.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18434..20107
                     /locus_tag="ACG8Z3_000884"
     CDS             18434..20107
                     /locus_tag="ACG8Z3_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0823222.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20121..21752
                     /locus_tag="ACG8Z3_000885"
     CDS             20121..21752
                     /locus_tag="ACG8Z3_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0823223.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22624..23814
                     /locus_tag="ACG8Z3_000886"
     CDS             22624..23814
                     /locus_tag="ACG8Z3_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFQ0823224.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYSPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23948..26137
                     /locus_tag="ACG8Z3_000887"
     CDS             23948..26137
                     /locus_tag="ACG8Z3_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0823225.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            26134..27927
                     /locus_tag="ACG8Z3_000888"
     CDS             26134..27927
                     /locus_tag="ACG8Z3_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0823226.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            28127..28441
                     /locus_tag="ACG8Z3_000889"
     CDS             28127..28441
                     /locus_tag="ACG8Z3_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0823227.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            28462..28839
                     /locus_tag="ACG8Z3_000890"
     CDS             28462..28839
                     /locus_tag="ACG8Z3_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0823228.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            28849..29622
                     /locus_tag="ACG8Z3_000891"
     CDS             28849..29622
                     /locus_tag="ACG8Z3_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823229.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            29644..31047
                     /locus_tag="ACG8Z3_000892"
     CDS             29644..31047
                     /locus_tag="ACG8Z3_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0823230.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            31229..32413
                     /gene="mobT"
                     /locus_tag="ACG8Z3_000893"
     CDS             31229..32413
                     /gene="mobT"
                     /locus_tag="ACG8Z3_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0823231.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            32410..32700
                     /locus_tag="ACG8Z3_000894"
     CDS             32410..32700
                     /locus_tag="ACG8Z3_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823232.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            32697..32918
                     /locus_tag="ACG8Z3_000895"
     CDS             32697..32918
                     /locus_tag="ACG8Z3_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823233.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            32960..33739
                     /locus_tag="ACG8Z3_000896"
     CDS             32960..33739
                     /locus_tag="ACG8Z3_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFQ0823234.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            33800..34300
                     /locus_tag="ACG8Z3_000897"
     CDS             33800..34300
                     /locus_tag="ACG8Z3_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0823235.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            34356..34994
                     /locus_tag="ACG8Z3_000898"
     CDS             34356..34994
                     /locus_tag="ACG8Z3_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFQ0823236.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            35066..35461
                     /locus_tag="ACG8Z3_000899"
     CDS             35066..35461
                     /locus_tag="ACG8Z3_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0823237.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            35445..37898
                     /locus_tag="ACG8Z3_000900"
     CDS             35445..37898
                     /locus_tag="ACG8Z3_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0823238.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            37895..39922
                     /locus_tag="ACG8Z3_000901"
     CDS             37895..39922
                     /locus_tag="ACG8Z3_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0823239.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            39919..40941
                     /locus_tag="ACG8Z3_000902"
     CDS             39919..40941
                     /locus_tag="ACG8Z3_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFQ0823240.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            40958..41887
                     /locus_tag="ACG8Z3_000903"
     CDS             40958..41887
                     /locus_tag="ACG8Z3_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0823241.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            42132..>42208
                     /locus_tag="ACG8Z3_000904"
     CDS             42132..>42208
                     /locus_tag="ACG8Z3_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="regulates the transcription of tet(M) by
                     attenuation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HFQ0823242.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDK"
BASE COUNT        13827 a         6985 c         8337 g        13059 t
ORIGIN      
        1 tgatttcaca acctctgatt cacggtgttc cagaagtagc ttctacggaa ataagccgaa
       61 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
      121 acttctgtca caacctctta ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg
      181 tctagcgcaa gagatttgct ataatagacg ataagggtaa aatgtaacga gaaggggttg
      241 aaaaatgtgt atcaaattgt aacagattct tgttgtgatc tgccatacca tgtattagca
      301 aaagaaaaag ttgatttttt atcaatgaat ctccaattga atgatcaaga gttagtagat
      361 gacctcggaa agacattcaa ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg
      421 cctacgacat cacaaatcaa tgttggccga tatttagaat ttttccgttc ttatgcggaa
      481 aaacagattc ctgttttata tgtggctttt tccagtggat taagcggctc ttacagtagt
      541 gcgcttcaag cggttgaatt gctgaaggaa gaatatgaag accctgagat ctatgttttt
      601 gatacaaaag ctgcaagtat tggggaaggg cttttagtat tggaagctgt aagattgaaa
      661 aaagaaggta aaagcatcga agaaacccta gagtggctgg aaagtaatta tttgaaagtc
      721 cattcatggg taacagtaga cgatctgaaa tatctagaac gaggcggtag gatttccaaa
      781 gcaagtgcta ctcttggcag tctattgagt gtaaagccta ttattacggt ggataaagaa
      841 ggaaggctga cgaatgttga caaagttcga ggaagaaaca agtcgatcca ggcaatcgtc
      901 aatgagacgg taaaaacaat cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct
      961 ggggatctag agagcgcgga gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa
     1021 gatgtgaagc tttatccctt aggaccaacg atcgccagtc acacaggcta tggctgtatc
     1081 gcgatttttt cgatgggaat cagtcgataa aagcagtaac tcattctgta aaaaagccca
     1141 gaaattgatt gtaacaaaaa cagcgactct taaaagatag atgagcatcc tatctcttag
     1201 aggtcgctgt ttttttattt tttaagctaa ataggccatt atgcccataa ctcgttcaat
     1261 tgtttttgaa tatctgcatt ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc
     1321 acacctttat ccgaaacggc aatgatgcgg tttgccaatg tttggataaa ttgatggtca
     1381 tgtgacgcaa ataagattga gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt
     1441 gattctaagt ctaaatggtt cgttggatca tctaatacaa gtacgttagc tttcgaaagc
     1501 atcatttttg aaagcatgca acggactttc tctccaccag ataaaacatt cactggtttc
     1561 aatacttctt caccagaaaa caacattctt cctaagaagc ttcttaagaa ggtgttatca
     1621 tcttcttctt tacttgcata ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat
     1681 tctttcgttg tatcctttgg taaataagaa ttacttgttg tcacacccca acgtactgtt
     1741 ccagtatctg gcgtaatttc acccatgata actttgaata gcgttgttgt tgttatatca
     1801 ttttctgcga cgaaagcgac cttgtcttct ttgctcaaat taaaagatat atcatctaaa
     1861 attttctttc catcaatcgt tacacttaca ttttctacct gcaataagtc gtttccaatc
     1921 tcacgttcag gtttgaatcc aacgaatgga taacgtctag aagaaggttg gatatcgtct
     1981 aaagtaattt tttcaagcat ttttttacgt gaagttgctt gttttgattt tgatgcattc
     2041 gcactaaagc gggcaataaa ttcttggagt tctttgattt gttcttcttt tttggcattt
     2101 gcgtttgctt gaagtttagc cgctagttga ctagattcca accaaaaatc ataatttcct
     2161 acataaagtt tgattttacc aaaatctaga tccgccatat gtgtacatac tttgttcaaa
     2221 aagtgacggt catgggatac aacgatcaca gtattttcaa aattgatcaa aaattcttcc
     2281 agccaattga tcgattggat atccagaccg tttgttggtt cgtctagcaa aagaacatca
     2341 ggtttaccaa atagtgtttg tgctaataac acttttacct tttgtccacc agttaattca
     2401 ctcatttgct gatggtgcaa ttcttcaggg atgttcagac cttgcagtaa aacagctgct
     2461 tctggttcag cttcccatcc attaagctca gcaaattctc cttctaattc agctgctcga
     2521 ataccatctt cgtcagtgaa atcctctttc atgtaaatcg catctttttc tttcagcact
     2581 tcatacagac gcttgtgtcc catgatgact gtttcaagta cagtatagtc ttcatagtcg
     2641 taatggttct gtttcaatac ggccatccgt tcatctggtc ccatagtaac ggaacctgtg
     2701 gaaggctgca gatcccctga taacacttta aggaacgttg atttacctgc accatttgca
     2761 ccaatcaagc catagcagtt tccgggggta aatttaatat tcacatcatc aaatagtttg
     2821 cggtctgaaa agtgcaaact cacatcattt acagtaatca atttttttcc tcacttttat
     2881 cattattagt tttgacaagt cttatagaat tatatagggg aaaggtaaaa gattcaaggc
     2941 aatcgaacat tctttgtgtt tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga
     3001 gcgaaaatag ctactatagg atttattagt ttgcaacagc ataatttttg acgttattta
     3061 taagagggag tactataaaa ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa
     3121 aagaaccatc gactggggct cattgatttt gggaatttta tttgtacttg tctcattgtt
     3181 atcatttcaa gaccctgtcg gaaatttagt tgcaattgta gtagtctttg cgatttttgc
     3241 ctttattaaa gggatatttg aactgtttgt acggaacaga ttaaaagagc taacaggata
     3301 taaaggaaag atgcccttaa taatcgggat catcgatatt ctagttggcg tctttttctt
     3361 attcaatatt ggtgcaggag tcgcagcatt gccttttgtt tttgcagtat ggtttattgc
     3421 tgattcagtt ttagcgttat ttactgctga cttggcaaga ggagtaagtg aaggatatta
     3481 ttggtttacg atcatcgtca atattttagg aattttatta ggaattttct tattgtttaa
     3541 tccaatttct tctgcgttga cattaagctt tttagttgga ttctatttta tgttatttgg
     3601 tatcacacat atcgtctatg catttagata agtaaaatca aacaaataaa aaagccggct
     3661 tccgattaca gttttactgt tgtaattgga agccggcttt tttatcatgg acaaatatgc
     3721 acgaggtgat ttttaggtgt cgagacaaat ttatgcaaaa atgtgagaag ctatatacac
     3781 aaattttatc ttaatactcg aagcatagcg cagttgtcac aacctctttt catataattc
     3841 attcaatact ggcaaatcat ggacttcata tttttctttg aatgcttgga tttcttcttc
     3901 agaatatttt cttggatcta aatgcagatc ttctactggc aatgggcgct tgttattgat
     3961 cttcacatcg attactacag gacgatctga ttttgcagct gcatcaaagg caaatcgaag
     4021 ttgcgagtat tctgtgactg taaagctatt tgccccgagc gcttctccaa ctttaccgaa
     4081 atctgcatct tctaaaaata caccaaattt cttttgttcc gtatcttctt gttctgcttc
     4141 gatgaaacca aaagaatcat tcgaaaagac aacattaata attggaagct gatatttgac
     4201 ttgtgtaatg atatcttgca tgaccatagc aaacccgcca tcaccactta aggtaaatac
     4261 ttgtttttct gggtagctta gttgtgcggc aattcctcct ggcacaccat tacccatagt
     4321 tgcgaaccat ccagatgtcg tatgtttttg tttaccgttc atattcaata aacgaataga
     4381 atggatagtc gtgttaccga catctgtgac gaaaatcgca tcgtcttcag caatacgatt
     4441 gatttcttta tagacagcct ctggacgaat cggttcttcc tctcgatctt caaaagattt
     4501 caaccaattt acccattttt ctttattttc ttgatttgct aggtaccagc tatcagcagg
     4561 gcgagcatcc cctaactcga caagtctttg caaagctgtg tttgcatcac cgaggatcga
     4621 caaagatgtt tcatggcgtc gtccaaattt tgacgcatcg atatcgattt ggataaattc
     4681 agcagttgga ttaaaaaatg cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag
     4741 atcggcttct gccaatgctt catttccggg tttagtagcg acacgtccag caaaaccaag
     4801 aaagttttca taatcatcag gaataattcc ttttgctaga acggaagcga caacaggaac
     4861 tgaaaaatgt tctgagaatt ctttgatttg cggaaatccg tttcgcgttc cttgaccaac
     4921 ataaagaact ggtttttttg ctttttcaag ataaggcaaa gcagctagca aatctttttc
     4981 ttctggtaat atcacacttg ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat
     5041 ttcttcaaaa ccaagatcaa cagggatagt cacgatagcc acgcctttat gctcataggc
     5101 ggctttaatg gcttcatcta ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt
     5161 gtatacgcta acatctgcga aaataggatt ttcatttagt tcctggaaag agttatagtt
     5221 catggatgta gaagcaactt gtcctaagaa cgctactaga ggcacatggt ccatctgtgc
     5281 atcatacaaa ccattgatca aatgtgtcgc accaggtcct gcagagccga agacagcgcc
     5341 gactttcccc gttaattttg catctgcagc ggctgcgagt gcgccgactt cttcatgacg
     5401 aacttgaatg tagttgattt cggttttttc ttgatatagc gcatccataa ttgaattgaa
     5461 agatcctccg ggaatgccgt aaatatggtc gataccccag ctttcaagaa cttttaccat
     5521 tgcgattcct gcattgattt ttgacatggt gtttcactcc tttagcaatg atgatacata
     5581 aattttttgt atctagagta tagaactttt tatcgaaaat cccaactaat tcgtcttttc
     5641 acaaaaatgt agtcatgttt ttgggaatat gctagagtag tacatagata gattcaagag
     5701 aggggaatgg catggttgaa tggctattga gacttgaacc ttggcagcaa gcacttgctg
     5761 ggacagcttt tacttatttt atgacagctc ttggtgccgg gttggttttt ttctttaaag
     5821 aaatcaaaaa agaagtgcta aacttgatgt taggatttgc ttctggtgtc atgattgctg
     5881 caagtttttg gtctttgtta gatccggcta ttacaaaagc agaagaaaat ggagatattg
     5941 cttggctagt tgtaagtatt gggtttggtt taggcggtgt gtttttatat atggcagata
     6001 aaacactgcc ccatatgcat tttggaccac aacatgaagc agaaggattg cctacccatt
     6061 taaaacggac gatcttgctt gttttttcta ttacattgca taacattcct gaaggtttag
     6121 ccgtgggggt agcattcggt gcagcagcaa ctgcggataa tccgacagcc gctatattag
     6181 ccgctgtttc ggtggcgcta ggtattggta tccaaaactt cccagaagga gcagctgtat
     6241 caatcccgtt gcgacaagaa ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt
     6301 ctggaatcgt tgaaccaatt gctggagtga ttggagcgct actagtaacg aaagtggagc
     6361 tgctgcttcc ttatgcactt gcttttgccg caggagcaat gatttatgtc gtggtggaag
     6421 aattgatccc tgaagcacag cagacattgt ccagcaaacg tcattttgca gtttttggcg
     6481 tcatgagtgg atttatcatt atgatgattt tggatgtggc attaggataa ataaaaaaaa
     6541 tatatatgat atatcaaata gggccgcgaa cttatatcgc gaccctattt ttttagtaag
     6601 ttatatcata atgaagtgaa tggtatcata cattaataat agttttttct atgtgtattt
     6661 cctttgatcc agcgaacaag ccagatgata agtgcgattg ggattagaac tttgaccgca
     6721 aaccataaaa gcccgcttag gacactgaca aaaagactga tgacgaagct agctgcaatt
     6781 agcactaaaa gaaccatgaa gatatttaag aacatatttt tttgacgcat acaaaaccct
     6841 cctcttgttc attctttact atagaaaaag tataacaggg attgtttaaa aacacctcca
     6901 actgaagact gatttttact tcatctactt aaatgatcaa acaattgctt tttctctggt
     6961 ttatcatcta aaatagagcg gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag
     7021 catttacaga tgctgtaatc gcgattatca tgactatttt agttttagaa cttcatgaac
     7081 ctaacggaga cacgtgggag gcgctcgctg cattaggtta ccgatttttt atctatattg
     7141 tcagttttat cattttagcg atttattgga acaatcatca tcatttattt caagttgtac
     7201 ataagataaa tggacgggtc ttatgggcga acagtttctt tgtttttgcg ctttcgcttt
     7261 ttccgtttgc aacttcatgg atcagtgagt acatcaatag cctggctcca gaaatgactt
     7321 atggttttgt taccttgacc gctaatattg cgtattatat cttggcaaaa gaacttgtca
     7381 aagcaaataa aggccacgag aaagcattga aggcgatgtt gaataattac aagaaatcgt
     7441 atctatcgat tggtttgaat gtgttaggaa ttgtcttagg gtatttagta gcgccagtag
     7501 ccgtgctgat cgcaaacatc gtgattctag ctgcttggat catcccaagt cgtaccatcg
     7561 aatcacaata tcggtaaaat gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat
     7621 aaataaacga tcaaatatcc gagtgatact agtattcaaa gagggatgca gatggatcaa
     7681 aaaagaattc atgtaggact gattactagt tttgtcacga tgcttattta tttggatcaa
     7741 ggtcatttga cttggacagt agttggtgtc gtaggatttt tgacattatt cacagcaata
     7801 aaaaaataaa catctttatg taatctgatg ttctatctat ttaagaaaag catgtttaca
     7861 gcagtatgat tctgttgtaa acgtgttttt atttgtggaa aatttaattt ttagaaaagt
     7921 gtatcgaaaa atagatagtt agtttttcca cagtggataa cttcgtaaga gataaaaata
     7981 aaaaattagt caaagaaggg cgtatagatt tcttttgata ggagatatac aacagaaatg
     8041 aataaatatt aacgtgtttt caaacagtat aaaacggtgt ggtttttgga ataaaaaaaa
     8101 taacatatct cattttacgc ttttgttatt ttatacttaa aaaataacga cacagaaaat
     8161 accgtgtctc agatttctct actacttcta aaaactactg tttttttcct ttgaaatgcc
     8221 ttcaaaaagt aggatcaggc agaatatact ggcagaatca aggagaaggg agcctaaata
     8281 aggagaaaaa ccatagaaaa tataaagtaa ttacttataa aacaagcttt ttatagaaaa
     8341 ataatatacc tgagtagggt ataaattaag acggaattcc tttctagatt gagatattga
     8401 ctaggaagaa tgttaagatt ttaagccttt aagctaaaaa aaaacccttt ttgttaaata
     8461 aggggaatat agcctgtcag tttcaaactg caagtcgtcc tgctgcaata atatagtaga
     8521 ttagagatga aaatagattt ttttaaacca atgactgaat aaaaagaccg tcatagatac
     8581 acattggaat gtgtctatta cggtcttttt ttacgaacga ttttattgaa gacggacagt
     8641 tcgggtcaaa aataaaaaag ctacgatcag aaataagaat aaaatgtatc ccaaaataat
     8701 ggctagacgg tctaacttat ttgtcgatag atgtttgttt agaagttgat aggcccataa
     8761 acctatcagt cctcctgttg tattagttat caaatcagtg atgtccgcta cgccaatacc
     8821 gaataggaat tgaatcaatt ctgccaagaa gcttacaagg aaaataatcc ctaatttttt
     8881 agaaaaagaa agtctcttga aattgacatt caaaagcacc ccaaaaggaa aaaagacaat
     8941 caagttataa gtggtctcac gtaataccgt cgtattttca aaagaaaaag tagaaaaagg
     9001 gacgagattg acattgctgt aagaataagt gagaaattgt ggaagattaa atgatagctt
     9061 gaataaaatc aaccataata gaaagaataa ataaatccct aataaaaatt ttgatacata
     9121 ccgcatagac atctcctcca tttaaaaagt ataccatcga aaaccgctga aaaatcataa
     9181 aaaagtatga agtttaagga ccaacgtagt tatcgtacga aaattagaag cttttttttg
     9241 ttgggaatcc ggtttcttta acaaagctaa gatagcgagt tgcttggaaa agttgctatc
     9301 ttttttctgt caaaacgact cggaatgttc gttttttgtt ctaatttctc caagttgttt
     9361 actttttgat actaagttta tgaaaaatca agaaatagtt gttatactag gtaatgctga
     9421 caaacgggat tggcatttca tcttggttca gctttttttc caattactag gaaggaggga
     9481 tagattgttc aagacttatt ttcgtttcaa taaaatacaa gataatcctt ttcgtatcat
     9541 gaatctgatg ctacttatct ttattgtatt atggttaggt tacataaatc atgatttttt
     9601 gatttcaagt ttttcatcat tagggatctt tacttttttc tattatcaaa acataccgat
     9661 gaaatatttg ctgaaacgta tgtttctgat cggttgcgga ctgttggttg ctttcgtttt
     9721 ggggatctta tcgacttatg ttttatggct tgaaccattt gccgtagctt tggttgcttt
     9781 ttccagcaga ttcgtgttgc gattattcca tatttctaaa ccaggtggct tgttttttgc
     9841 gatgctttct gccatgggaa cgagcatgca gctgccaatc gctcaattac cgatcgtctc
     9901 actttatttt tttatgggtg tcgtttttgc cctgattgcg gcagtgatta caaaattact
     9961 ggattcacgg cctgagcaga caattgaaaa agcaacttta aaagaaagat ttcacgaaga
    10021 gcctttagtg atcattgatt ctgttttcta cagcgctgcc ttatttcttt ccgtttatgt
    10081 cagtcacggc ttgaatctgc ataatcctta ttggttgacc ttgtcatgtg catcgatttt
    10141 gctagcagaa aacttggatg cgatgaagca tcgacaagtc caatatctga ttggatcgat
    10201 gggtgggtta tgtgtgtcgg cattcttgtc atttgtgccg tttacgcagt tgcagaccat
    10261 ttttctgatc acttttctat atggaatcgc acaatttcta gttgctagaa attatgcagt
    10321 tgctaatatt tttcttaatc cgatggcgtt gatgctatct acacttattc gtggagcata
    10381 cttgatttct ttgatcgaat atcgtttttt agggatcgtc ataggaagtt tcatcggttt
    10441 aggtgttgcg tgggtaatga cggttggttt gcaacattat ttaacggttg ttcgtcaaaa
    10501 attagaatag aaggacaaat atatgtgcca aaagaggtta tgacaagatt tttgtcacaa
    10561 cctctaattc aaggtgttca aaagccagat tctcagcatt aagtcaaatt cacacaaaaa
    10621 tgtggaaagg aactttcaaa aaaattttat atttattcta tcaaaaaatt ttatatttat
    10681 tctatcaaaa aattttgttt atgatggtaa ctgaataaac tttcctgaga tggtcaatgc
    10741 tttccttgaa agtcttacag atattctttc ttcatagaaa gtttaagttt tatagttagt
    10801 gtacaataaa agtatgttta taccatatgg tgtccggata atcagtcatt taaacaatga
    10861 ggttcaaaga tatgaaaagg ggaaatttga tgaagaaaat aagcaatgaa atccaaattc
    10921 tggcagaaac tttaactctt cccaaagtcg ttatagagca aatgattgta caagcaaaaa
    10981 acatccttga tgaggtgttt gagcaatata gtcatgaact aatggatcct aataaagcaa
    11041 aggcgactgc aaagaaaata gacaaagtct atgaagagga accttttgca gcattagcta
    11101 tttatctata tgcagcttct caaagttgga aaaaaattta tcaaccattg aagattccaa
    11161 gaactatcta tttggcaaca atgaatgctt ttactagatt tattcatgaa catttccaag
    11221 taacaggaag ctatcgcttt gaccgtggtt tttggatttg gcattacact agtggcttga
    11281 tctttcggat tggtgaattg gagtacgaaa tgacatattt tccgaaaggg tacacggatc
    11341 taagattaga aggaaagcag tgcctttctc ttcatattcc ttccgatgca gatttatctc
    11401 agaataagat aagagatagt tacgaaagtg ccattcgttt ttttgaactt tattttcctg
    11461 attaccatta ccaagtgatg tacacagata cttggttact atcacccaat cttactaaat
    11521 ggttgaaaaa agagtcaaaa atctgtcttt ttgcagccga ttatcgatta ttatcagttg
    11581 atgaacagga cgatagtgga gtcccttgga tctttggacg agtagatgcc caaatacatg
    11641 attatccaga aagtacgagt ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc
    11701 acattggctc tggcttaggt attcttactt cataagttta gtacttcgaa atagtttaaa
    11761 agagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
    11821 ggaagcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
    11881 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttagt
    11941 gttctatcga acacacagta cgttcaatag gtaaactagc tataacaata gatattaaag
    12001 ttaacggaca agagggcctt ttttaggtag ctaaaacgta ataatgaaaa tttatttcca
    12061 gagataggag tcaatcagct agtctgatcg actgttttaa caagttgact ttcattttaa
    12121 cttttgatac catttaaaac acaaatcgta attgtttcga tttgtgtttt ttagcatgac
    12181 tacaatcaaa gaagaaagtg ggtcgtccat atggaaaaaa cagatatctc aagtgcatac
    12241 cgccgcttga aaagcccaaa tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat
    12301 gtgaaacgga atagtgggaa aaggtaagaa gaaggaaact taattaaaaa aatagccgaa
    12361 aaacgttttt cggctatttt caataatcgg tttttgtgta aggtttccga aagaactgct
    12421 gttgattttc catttttaac atatgataaa ttggtagaat ctttcttttc aaattgatac
    12481 cattccctaa ttccagttat atagttaact acagaaccac aaagcacgta cgtttataaa
    12541 agtattatct gtagttattt ttcaattccc tttcaacctc ttattttgac cagtagtatt
    12601 gatcacttta ccttttaaca cctttgttcg tttttgaaac atttttagtc aagaatggaa
    12661 acgtaactac ataatcaaag gttttgcgaa atatttcatg aaatatttca tgtagaagcg
    12721 agcaatacgg ttgcaaataa ccattatcgt ttaataagca gttacgaaag ctcccaatat
    12781 ctctaacgtt cagctcactc catttctttt cttcaaaaaa atcagtgatt cgtatctctt
    12841 tctatttgtc gaaaaagtta actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa
    12901 aaaagcagtt actattgcta gaataacatc aaggaataat aagttttggt tttcagataa
    12961 tagtcgataa gttgtgtatt ggaattttag atacagtaat aaagtatttt tcatgaaatt
    13021 ttgtgagaat cactattaca agtatcttta ataaaattaa tggttaagga ggaacttaaa
    13081 gtagaatgta aatgaatagt tgtagtcagt cgattgtgat actagactca aagttgcagg
    13141 aataagtttt tcaatttgtg attggaagat aaaaaatgaa aaagaagggc ttttttttat
    13201 actttgttga ttgtagtgtt tattttccct ctgtttagcc aagtaacatt ttctatgact
    13261 caaccagttt caagtgagga acatgataac ctttttcgtg ctacagaaga gtttaagaaa
    13321 tatcaggaaa atattgttcc ggagactcca ttagctagtg agccaattac tgatattgat
    13381 ggaaaccaaa taggatggta tatacagtac gaagttatgg tggtaaataa tgactcaata
    13441 gtaaaaaata cagtaggctt agagctaaca ccgattgaaa aggtgagttc attgttaacg
    13501 tatatttata ctgatgaaag tgacgagact gagatcattt taacggatta ctcaaaaatt
    13561 gctactgata aaaagtatat attgtggatg tagacactag tgaaatactt tatgatatag
    13621 aaattgatga aaatttacta gatgtagcac aagaaatcga tcatcaaaag caggaagcta
    13681 ttcgatatgc tggtagaaaa gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt
    13741 agagaagatg aaaatgagtg gaaagaattc aagagtcgct actcaattaa ctcgactccg
    13801 acatttacag tttacagaga aggatcgata gaaaaaactg ttttttggac taaagaaagt
    13861 ggaatgtctt tagctgaagt agaagagttt ttggattatg ttagtatgca acagtaaggg
    13921 agttcttatt atgaaaaatt ttgacacata ttatgttatt gttttttcga tatctttgct
    13981 gttaatatta ctaactttaa tgcagtataa agagtatcca gagtatttta ttttaacttt
    14041 tgtatattta gtaagtagct acacacttct atataaattc agaagttctt ctagagtttc
    14101 taaagtttgt ttcttaatta gcttattgat tctataatat ttttgattta tataggaaat
    14161 taaaaatatt atagaatcaa cactcagaga acgggttggt ttttaataga tttaataagt
    14221 actgataatg aaatttctcc tggttgggaa aacgaagttg gttaccctgt agaagaattt
    14281 tttcaagatt actttgcgga gtatgccgat aggataatga gagattacgt aacagataaa
    14341 actcaagaag tcatcaatca atcccagtca ttaaatgaat tatctgataa ggcgaaagaa
    14401 gaagcagttg ccattatttc ttcagggatt agtgactata ttgatatgag tggtgaaatc
    14461 aattatggcg ataaagacaa aattgtacgc atgataaaag cttttatcaa aacttttgat
    14521 gtgaaggcag atcttaagtt taatgatact tacctagtag gaaaattaat taatatcctt
    14581 gggacagaga aagatacgga aacgtttatt tcctctttgt ctatgttgtt tacagataag
    14641 catttttcaa ctcagcttgg tggagaggaa ctagtggaaa tcttttaacc attacgccca
    14701 gcattaataa aagtatatca aaaaagtact cctgatgatt tttaagattg gtttgagaaa
    14761 taatgtgaaa acactatttt taatgttaat tatagataga gatgaacgcg tcatatggtt
    14821 aaaagaatga aaaagtcaaa gatacactgc tatatttcac taaggatata gcagtgtatc
    14881 tttcatgtcg tttcactggc agttttttat aaaaggaggt gtgcatttca gttgcttttc
    14941 tttggtagtt agttgaatcg tttaagaatt aacggatgtg gcagacaggt gctctgtcca
    15001 aaaaccagta aaatctttga tggttccatc tttatttagc tgtgcctgtt caaatactgt
    15061 tttgttttta ccgaatggat caattgcaat aattgttccg atccccgcac gtttggcagc
    15121 aagtaatcct gagtaagcat cttcgataac aagacaatca gccggagcta agctcagttt
    15181 gtctgccgct ttaagaaaaa tatccggttg cggtttccca gggaaagagc cgtcgtcata
    15241 gacaatcttg tcccagtcaa accagcgggc taaatcgaaa atatcaaaat aaaaagcaac
    15301 attctctttg acagtagccg tggcaatcgt taatggaatc tgttggtcag tcagctggtc
    15361 aagagtcgct tcaagccctt ttgtcaactg aagctcgttt tcattttgca agcaaagcat
    15421 acgataatag ctttcttttt cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc
    15481 tgaaatgaaa tgtttcaaaa tctccgtatt cgtccgacca tgaatatttt ttaaaatatc
    15541 ctcgtctgat aaaccatctt ttgtatgatc atgtatcata tgcagccaag cagcttcatg
    15601 cagatgagag tccaaaaaca ttgttccgtt gaaatcaaat ataatccctt tcatagttac
    15661 gctcctatag tggtattttc ggttgaatca gcaatttgca gatcgacata ttgtgcatag
    15721 tgctcttgaa cctcccgcga gatctggcga tccgtaatga gcagatcgaa atcatgcaaa
    15781 ttataaaaca cataaaaatc atctcgattg aattttttat tatcaacgag tagatatttc
    15841 ttacgggaat tatttagagc gatctgctgt gcttctcctt cttcggtact gtaagtagag
    15901 atcgcttcgt tgtgaacgcc atttgaactg ataaatgctt ttgtaaactt tagtcttctt
    15961 aaattgtcgt tggtgatcgg tccgacaaat gcgccggtat ttttccggaa atcaccgcca
    16021 atcagtagaa tttctggtcc atctgcttga gcaagaatat caaaaactgg ataattattt
    16081 gtaatgatcc gaattttctt gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct
    16141 ggtcctaaga aaatcgtttc tccctcgtga atcaaggagg ctgctgtggc agcaatttct
    16201 tttttttctt gaatctgcag agtggctttt tcagtgtgag aaagttcttg atctaaagtg
    16261 taggaaagac tttgagcccc tccatggaca cgcagcagct tgcccgattt ttctaattca
    16321 tctaaatctc tgcgaacggt catatcagaa acatctaatt gattgatgat ttcattaacg
    16381 gtaaggattc cttttttatt gaccatttct acgatagtaa gcaatctttc ttttttcaac
    16441 attgtcttca cctctaattt aaggataatc tatcatataa acggacaaca gtcaatctaa
    16501 ttcgaacaaa agtaaacatt tagggtggtt tttatgtttg ttacaataga ttttattgct
    16561 gttttctggt ttttatgatt atttgtgttt gtttttgttg acaaatgttt tggatgcgtt
    16621 tacaatgcaa tcagttagaa agagcgaaaa ctgattatag atttgaggag agatgtacat
    16681 gcttaaatta ccagaagatt ttatttttgg cggagccaca gcagcctatc aggttgaagg
    16741 agccactaag gaaggtggca aaggcgctgt tgcttgggat gactttttag aggaacaagg
    16801 gcgattcagt cccgatccgg caagtgattt ttatcatcaa tatgcaaagg acattgaatt
    16861 gtgtgaacgt tttggtgtaa atggtctaag attatccatt gcttggagcc gcatttttcc
    16921 tgatggagct gggaaaccaa atccagaagg aatcgctttt tatcatcgtg tgttcgaaga
    16981 atgtaagaaa agaaacgtaa cgccgtttgt gacgttgcat cattttgata cccctaaacg
    17041 tttgtttgac catggagatt ttttgaatcg agaaacaata gaagcatttg tttcctatgc
    17101 cattttttgt tttcatgaat tcaaagaagt caaggtgtgg agtaccttta atgaaatcta
    17161 tccagttgca accaatcaat atttgttagg tgttttccca ccaggaatca aatatgattt
    17221 tacaaagatc gtcgcctgtc ttcataatat gatggttgct catgcccgtg tagtgaacta
    17281 tttcaaagaa aacgaacttc cgggtgaaat tggtgtagtt cactcattag agacgaaata
    17341 tgcggcaaca gacgcgccgg aagataagca tgctgccttt ttagacgatg cattgtcgat
    17401 ccgtttctta ttagatgcta cctatttagg ctattattca actgaaacct tgactgctct
    17461 tgatgagata tgtgaagcaa atcaggccag ctatcatttt cctgaagaag atttcgttga
    17521 attgaaaaaa gcatccacgc gtaatgacta cttagggatc aatcactacc aatgccattt
    17581 tgtcaaagct tacgatggag aaaatgcaat ccatcataac ggtacgggtg aaaaagggac
    17641 ctctgtttat aaagtgaagg gaatcggaga acgtatttat aaagaaggaa ttcctcgaac
    17701 tgattgggac tggctgattt atccagaagg tctatatgac ttgttgctgc gaattaaaag
    17761 cgattaccca cattacaaca aaatctatat cactgagaac gggatggggt ataaagatca
    17821 gtttgaagat ggcattatta tggatcagcc gagaatcgac tatttgagag tctatttgga
    17881 atccttaagc aaggcgataa cagccggggt aaatgtgaag gggtattttt tgtggtcgct
    17941 tatggatctt ttctcatgga caaatgggta taacaaaagg tatggcttgt tttatgttga
    18001 ttttgaaacc cagaagcgtt acccaaaaga atcagcttac tggtacaagt tggtcagtga
    18061 aaccaaaacg attatttaat aagtgataat ggaggaagaa aagatgaata aagaggaatt
    18121 acaaatgctt ggttttgaga tcgttgcata ttcaggcgac gctcgcagta cattattgaa
    18181 attattgaaa gaagtaagac aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc
    18241 cgacgaaaat ttaacccttg cacataattc gcaaacaaaa attttagcgg aagaagcatc
    18301 tgggaaagag atggagatgg gattcatttt tatccatggg caggatcatt taatgaccac
    18361 tttattgctg agagatctga ttcaagactt tattgtactg tacagacaga atcaaggata
    18421 ggagaagaac gctatggatg gaatcattaa acaaatcgaa aaagggaaac cattttttga
    18481 aaaaatctca cgtaatagtt atttaggagc cgttcgtgac ggatttttaa cagcgatgcc
    18541 agctatttta ttttcaagca ttttcatttt gattgctgcg gttcctgaga tttttggtta
    18601 ccaatggcct gaatctgtct ccacatggct atggaaagtc tataattatt cgatgggtgt
    18661 tgtagggtta ctcgtttcag caaccactgc tcgctgcttg gctgattcga tgaatcgtcg
    18721 cttaccgaaa aacaaagtaa tcaacaccac ctctgtgatg ctggcctctg tggtaagttt
    18781 cctgttgtta agcgtctctc cgattgaagg tggattttca acggaatata tggggacaaa
    18841 gggactactg gcttcctttg tcgctgcatt tttaacagtt aatttatata agttttgtgt
    18901 gctgaaagat attaccatta aaatgcctaa agaagttcct ggaacgattt cgcaaacctt
    18961 ccgagatatt tttcctttct ctttttcagt atttgcggcc gttttaattg atttaggcat
    19021 tcgacaaatc ttccaaatct catttgcaga agcaatcatt acggtgctgc agccgctatt
    19081 tacagcagct gacgggtact taggaatcgc tattatctgg ggagcgatgg cgctgttttg
    19141 gtttgtcggt gttcatggac cgtcgatcgt ggagccggca attgcggcaa ttatctatgc
    19201 caatgtggaa accaatctgc aattgttcaa ttccggtgaa caagcttcta aagtactgac
    19261 ggtgggttta gggaattttg taggaactat gggaggaact ggggcaacat tggttgttcc
    19321 ttttatcttc ctactctttg ctaagtctaa gcagttgaag gcagttggga aagcttcctt
    19381 tattccagta agtttcgctg tcaacgaacc attgctattt gcggcaccca tgattttgaa
    19441 cccttatttt tttattccat ttttgttaac acctgttgta aacgtttgcc tgttcaagtt
    19501 ttttgtcgat gtattgggca tgaacagctt tatgtacgtt ttgccttggg caactccagc
    19561 accgatcggt ttggttctag gaacgggcat gggtatctta gcttttgttc tagcagcggt
    19621 tctaatcgtc gttgacttcc tgatctatct accattttgc aaagcatacg atgctgtctt
    19681 agtagagaaa gaagcacaaa aagcagaggt gatcgcaacc gaggaaggtg cttctgccga
    19741 tgtacgtgag gaagtacctg tggcagcgag tcaagttatc agcgatgcaa ataaagaaat
    19801 caatgtgttg gtactttgtg ctggcgcagg gacaagtgcg atgttagcta atgctttaaa
    19861 aaaaggtgca tctgaatcga aagcaccgat cagtgcaatg gcgggtgctt atggttctca
    19921 ttacgaaatg ctgcaggatt ttgatatggt ggtcttagca ccgcaagtca attcttacta
    19981 tgatgatatc aaagaagaca ccgatcgctt aggaatcaag ctggttgcga caaaaggagc
    20041 agaatatatc ggattaacga gagatcctga aaaagctgtt cagtttattc tgaaccaatt
    20101 tcaataaagg aggagccata atgaaaatgg gaatttggtt gcgtaaagct gttttatttg
    20161 cagctgtgtt aggggctttg ggggctgttt caaaaactgt tgaagcatct gccagcgaat
    20221 ttattcgcgg agcagatatt tcgatcctgt cagatatgga aaaatctggt gcaaaatatt
    20281 atgaagacga tatttcaaaa gatgccttag agattttaaa aaataatggt gtcaactatg
    20341 ttcgactgcg tttgtggcag gatccttata atagtaatgg agaaagttat ggagcaggga
    20401 caaatgatct ggaaacgacg attgaccttg caaaacgagc aaaaaatctg ggacttaagg
    20461 tgctgctgga ttttcactac agtgattttt gggttgatcc tgggaaacag aatcttccta
    20521 aagcatggca aggtctgacc tttgaagaaa tgaatacggc attgtacgac tatacaaaga
    20581 acgttctgtc ggaaatgaag cagctggacg tttatcctga tatggttcag atcggcaatg
    20641 aactgaatag cggaatgctc tggccttatg gcaagtcttg gggagaaggc gggggagaat
    20701 ttgatcgatt ggctgctttt ttgaagtctg gtatccaagc tgttcgagac actcaaccta
    20761 aaacaactcc agtaatgctc cacttagctg atggtggcga tacaggagca tttacttggt
    20821 ggtttgatga aatcacgagt cgaggtgtgt catttgattt aattggagtg tcgtattatc
    20881 cgtattggca cggttcaatg aatgaacttc aaaccaatat gaatgcgatc agccaacggt
    20941 atggtaaaga agtgatcgta gttgaaacag cttatgggca tacaacagcg aatgccgata
    21001 ctatgccaaa tgcttttggt gaagccgaag cagctgcggg gggatatgaa ccaacaccga
    21061 caggacaagc agagtatttg ctggatcttg ccgatcggat tcaggcagta ccgaataatc
    21121 gcggggcggg cttcttctat tgggaacctc tttggtacaa cggaaatgtg agttgggcaa
    21181 cacaagcagg tatgaattat ctcagtgttc aatccactat gggaaacgaa tgggacaacc
    21241 aagcgatgtt tgattttgct ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag
    21301 gggcgcaaat aaatctagtt aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc
    21361 cttccaattg gcaaacctgg tttcaacaag gaaccgctaa gggtacgatc aaaacggaag
    21421 cgggcacttt ttttggggac tacaaactaa ctttttggca ggatcaagca tacgaggcat
    21481 cagcttatca aaatgttagt ggcataagca aaggaactta cacattgagt gcctatgtaa
    21541 tgtctggggg agagtttgat actaatcagt tatatatcaa aaattatggt ggagcggaac
    21601 taaatactgc ggttgttaaa acaagtgaat ggacaaaaat tacaatcaaa gacattccaa
    21661 ttacaacagg tgtttgtgaa attggggttt atagcaaggc ttcaggaaat gcttggacga
    21721 acgttgattt tgtttcattg gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg
    21781 aggttccctt cagccacatc gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt
    21841 tgccaaaaat aaccagaaac gatttccagt catttttacc aaaacgagta aagcaccaga
    21901 cttcatctac actttgtatt atgctaaaga gagttcaacg atttcgtaag aagcaatgaa
    21961 aaaattgcaa aaagttgtgc agataacata aagtttgttt aagaaagggt aaccttagaa
    22021 ttccgtcgtg aatatgattt gagcacttag atggatagaa aaaagttctc ggaaaacagt
    22081 tcgtttttcg agaacttctt tttatgataa gaatgaaaca aattaactta tattatagtc
    22141 tcttttggta gggaaccatc ttatttttaa tgaaaatcaa gcattttagc tattgaagaa
    22201 tgttgattta tagggattcc ccctccatat tatgttggct ctttattccc actctgtttt
    22261 caacgtaaag tgtataaaac agaatgcgat actatcaagg gagttaaaac aatatacaga
    22321 acagtaaaac actataaata aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat
    22381 catactctgt ttatatatga aaactagatt ctgaatccaa tatttggtta acgagaggcg
    22441 ttcgaatttg aacgtctttt atatattact ttcaaataaa tatgaggtag aatactatct
    22501 attttctttt taacccaatt gttataatgg agagtgtaaa atattttgtg taaatagaaa
    22561 aaaggaagtc ccttctgtag aatagagtta ccacaacaca ttcacagaag agaggacttc
    22621 cctatgaacg attttactac agaaattcta aagactctag cgaacaaagg cgatttgaat
    22681 gaattattcc gtgtccattt ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg
    22741 gctttcctag attacgaaaa gtatgatcgc attggtttta acacgggtaa ttctcgtaac
    22801 ggctcctatg accgtacggt caagaccgag tatggggaac ttcatctcca gattccgcgc
    22861 gaccgcaatg gtgagttcaa gcaacagact gttcctgctt atagacggac gaatgacacg
    22921 ttagaggaga ccgtcattca cctcttccga aaaggtatta ccatgtcgga aatcgcagac
    22981 ttgattgaga aaatgtatgg gcattactac tcgccccaaa ccatgtccaa tataacaaaa
    23041 tcatttacag aagaggtaac ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct
    23101 atttatatgg acgcaacgta tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt
    23161 catatcgcag ttggcattcg cccggacgga tcaaaggaag tattgagcta tgcgattgca
    23221 ccgactgaat ccatcacgat ttgggaggaa attttattgg accttcaaga gcgcggtttg
    23281 aaaaatgtcc tcctgttcat cacggatggc ttaaagggga tggtaggagc gattagtcgg
    23341 ttctatccca aagctcgttt tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat
    23401 aaagtgcgtg tcgatgatcg taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc
    23461 tcatctaaag aggtggcgtt ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc
    23521 tatccaaaag tggttgaatc gattctttcg aacgatcact tgctcacttt ctatgatttc
    23581 cccttggcca tacgcaagag tatttattct acgaacttga ttgaatcctt taataaacaa
    23641 atcaagaaat acagccaccg caaggaacag ttccaaaatg aagagtcgat ggaacgtttc
    23701 ttagtctcgt cttttgatac ttacaaccaa aaattcctag gtcgcagtca taaaggtttc
    23761 caacaggccg aaggcgagct cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa
    23821 tctgcagtag ggaagaatca tttacacaaa attattgacg ctcccttata atgagaactt
    23881 ttactaacat aatttatgat aaacttacta tataatcata ttaattaaat agttaggaga
    23941 tctgcttatg tttatatcta ctcttcaaat tcgaaatttt agaaattatg aatccgaaat
    24001 ctttcgattc gataatgaga caaatactat tataggtgag aatgattcag ggaaaaccaa
    24061 tgctcttact gcattgagaa tcttattaga tgatagttat tactatagta gtaaaacact
    24121 aaaagaatca gatttttttc atggaattca aaatgggtgg caagggcatt ggattattat
    24181 ctctgcgaca tttgaaggaa tcagcgaaga agaatttgac aatgagattt gtgcttccat
    24241 ttctttaaat tctgaaagtc aaacaatctt agaagaattg atttcaaatg cagataaagg
    24301 tgtaggtagt atttctctat ttattagacc taacaaagct attagaaagc aattatttga
    24361 tatttccact gaattagact ctcatcaatt taacgaattt agaaattcta ttcgtctatc
    24421 cgactatgaa ttttactaca catctaaatc aaatctaaat ttctgtatca atgaaaatta
    24481 tgataaattg gtaggaagac tcaatgacaa tatagctagt aatcctgaag aagacgatga
    24541 agctctgttg ggctcaagaa ttgacatgtc agatattttc aagcatattt cggttgttta
    24601 tgttgacgct cttcgagatg ttcttagaga aatgaaaaac aatcgaaacc cagtcaaaag
    24661 aatcatggaa acaattgaat caaaaatatc atcagataat gttgagtcat taaaaacaat
    24721 tatacagcaa ttaaatgaga ctattaccag tgttcctgaa attaggaaaa ttggcgaaaa
    24781 tattaatcgg caactaaata gcataattgg ttcagtgtat tctcctaatc ttcttctttc
    24841 ctcaactatg tcagatgata tgggttcttt agcaaaattt ctttcaatga agccagaaca
    24901 aaatatcgac ttagacttac tagggttagg acatctaaat atgatttatt tagctttgaa
    24961 aatagtcgaa tttgaagcgt gtcgttcaag agaactactt aatattatgt tgattgaaga
    25021 accagaagct catattcatc atcatattca aaaaactctg ttcgaaggat taaatcttca
    25081 aaaaaactat actcagattc tcatgaccac acattctgta catttagctg aagtgtctga
    25141 aatatctcgg atgaatgtat taaaatcgtt taatggcaaa tctatcgtta tgaatccagc
    25201 aaataatctg aactcatttg ctaaagaaaa gttaaaaaag aacaatctaa atctaataaa
    25261 cgcagtagag agatatttag atgttaaaag aaatggattg cttttttcta aaggtgttat
    25321 cttagttgaa ggagatgctg aagaaatact tattcctcaa atagtaaaaa aattatttgg
    25381 tataaactta gacgaacttg gaataggttt gattaatgtt ggtagtactt cgtttgaata
    25441 tatcgctagc ttgtttgacg atgatagaat acagagaaag tgtgctattg taactgattt
    25501 agatgaacag gcaatcccat cagaacatcg attgtataaa agcactgcac aagagaaagg
    25561 tcaagcaaga aaagaaaaat tatcctccct ttttaatgaa aacaattggg ttaattgttt
    25621 ttatgctaat acaacatttg aaattgagtt tttagatgtg aataaagaaa atattctaac
    25681 ttatgtcagt ccaattatag atgacgcatt caaagataaa aaaacaataa ctgcttataa
    25741 agaagaaata caaagtgaac aatatttaga aagaaattta gcgatgttaa aactagccaa
    25801 tcatgttggt aaggggtggt ttgcaattga gttaggtgaa aaaattgact ttttaatcaa
    25861 aattcctaag tatattctta atgctattgc ttttgcatgt caggaaatag tagatctctc
    25921 aatttataaa aaaattctac tccatacaat cgacttgtac gaggaaacta cagaattgtc
    25981 agctttatca gctagtttaa aaacaagtga tacagctgat ttgaatatag caataatttc
    26041 tttattagag tactttggta aagatgagga tgttgtgtat ctaatcaatc agatagaaaa
    26101 ttacacaaaa atactttatg ctgaggtttc aaaatgacaa atatcttcga aaaaatcgta
    26161 aatgatttaa atttagaaca aaaagatgtt gtggaaagtg aagataacct ttatgtaaat
    26221 gcatgtcctg ggagtgggaa aacaagggta cttacaagga aaattgctta ccaagcaatt
    26281 aaacacgatg gatcactaaa aagaataatt gctatcacat acactaatag agctgctgaa
    26341 gaaattaagg aacgcctaag tttacttagt atagatgatg atattaatat ttgggtgggg
    26401 acaatacatc agttttgtct tgaatttatt atttatcctt ttaaaatgaa tctctcaaga
    26461 gtatcaaaag gatttacaat tattgatgat tatactcaaa gagcttacgt cagccaaatt
    26521 aataatatgc tgaatttaaa tctcaaaacg tatgaactga acaaaattga tttgtcactt
    26581 tctcatgatt ttaaaattaa tgaagttcag tatactcaag tttctgaaat gtatcacact
    26641 atgttggaaa aaaataaaga aattgatttt gatatgattt tactagtagc gtataaaatt
    26701 ttaactgtaa acagtaacgt tgcacgtaat attgctagta atataagggc gatttatgtg
    26761 gatgaatttc aagatactag agaattacaa tacaatgtaa tagctaaatt acttcaaaac
    26821 aaccctcaaa ttcagtcaat gtttgttgga gatattgacc aagcaattta tggatcatta
    26881 gatggtatag ctaaatcagt tgaagaacta gaaatgctaa ctagtcacac ttttcaatca
    26941 aagacgctac atggctgtta caggtctaat cagagattag tagacttcta ctctaatttt
    27001 cagagttgtc cttacgagat tgaaagcaga gggaataata aaaacgaccg tggttttata
    27061 agttatgatt gcaaagttac taaggaggat ttacccaaca taataaaaaa tataataaag
    27121 gaaaagattg aaagcggcat tcatgaaaaa gatatttgtg tacttgctcc tcaacactat
    27181 cctctgtttt cactgggtga gtatttaaag aaagaattac cgtattgcaa ttttgatgct
    27241 ataaatatat caccaattaa ggtaaataat catagtttat ttttcaagtt agcaattttg
    27301 tactttactg aatcaggtga aaaggttcga agaagaaagc taattgcaaa cgatatttta
    27361 attatattaa aaaatgaatt tggaatagaa gttaaagatt attttattga cttagattta
    27421 ttacagctaa taaacagtgt gaaaagaatg tatagtggag acgacaacgg tgttgattta
    27481 tacaaatttg ttaccaaaca tatatttgtt aatcttaata ttgacgagaa aaattacccg
    27541 tctattttta agcactatct attttttatt aacgaagtag aagatcggat aaaaaataat
    27601 tcattatcta gtagtgtatc tagttttaag aagatgttta aatctcgaga tggtatcaca
    27661 cttactacag cacataaagt taaaggaaat gagtatgata ctgttatcgc tattaatctt
    27721 ctaaacggga aaattcctca ttggaatgaa atttttaatg tttctgataa aggaattagt
    27781 tcagcgagaa aactactata cgtaatttgt tcaagagcaa aaaataattt atttcttttc
    27841 tcagaggtgg gttattttac gagtaaagga gacttgcttt atcccactca attattagat
    27901 aaaatagaat ttgattataa tatttaaaat acctaggtag tcgttgtttt aaatatagta
    27961 ggaatcggac gctcagtacg gagcgtctat ttttttaccc aattttgaaa ggaagtgaac
    28021 ttatgaaaat aaaaaatcaa gaatcaaaag gtcgctctcc cccctttaaa accatcacac
    28081 aaataagatt cattcaaaca taaaaacaga aaggataggt aaaaatatgg aacttaaata
    28141 tgtcattccc aatatggaaa aaacatttgg acaattagaa tatgctggtg aagggaacat
    28201 tgaacaacgt cgaataaatg gcagaccaac cattctctct cgtagttaca atttatactc
    28261 aacgattcaa cgagcagatg atattgttgt cattcttccc tccgaagctg gcgagaaaca
    28321 ttttgaatcc gacgaaaaag tcaaactcat caacccacat attaccgcag aaggttataa
    28381 aattggcaat cgaggattta ccaattacat tatgcacgca gacgatatgg tgaaagcata
    28441 agaaaggaaa ggtgaaaaaa tatgagatta gcagaaggta ttgtgattga aaaagaccag
    28501 acatttggca cattgaaatt ttccgccttg cgtcgtgagg ttcgtatcca gaatgaagat
    28561 ggtacggttt ccgaagaaat taaagaacgt acctatgact taaaatcaaa aggacaagga
    28621 cgcatgatac aagtcagtat ccccgcttct gttcctttga aagaatttga ctacaacgca
    28681 gaagtggaat taatcaatcc tgtggcagat accgtagcga cggctacttt tcgaggggca
    28741 gacgttgatt ggtacgtgaa agcagacgat cttgtattaa aaggacagac aaaacctcat
    28801 cagaaatata aaaaagaagc gtccgaaagt gaaaaatagg tgttgcttat gacaaacaca
    28861 ccattgattt tgaaagaaat tccgaaggat gaagccattt cttttattcg gcaataccat
    28921 tattcaaaga tactccctag attgtgtaaa tactttctag ggattttttc agaagaaaaa
    28981 ttattaggcg ttgtggaatt aggctggggt acgcaacccc tacaaaccat acgcaaactc
    29041 tttcccgaca gttcccttca aaccacagat tatttagaaa ttggaaagat gtgttttctt
    29101 ccagaaatga atcaaaccaa ttattttggc agtcaagctc tttcagctct aattaagtgg
    29161 ttgaaagaac atacggactg tcatttttta tatacactgg cagatggaat tgagggaaaa
    29221 tgtggctatg tctatcaagc gtccaacttc ttttactgcg ggtatttcaa gacaagtgtc
    29281 tatcgtgata agcagtcatg ggaaaagata caccctcgaa gtgctcgcct tttattagag
    29341 gaaaatgctc gctttgaaca ggtagagaaa aagcattggc tttcccaggc attttgcgaa
    29401 tacaaaggga ttgagaaaat caatggacgc atgtttcgct atctctatcc gttgacaaaa
    29461 gaagcgaaaa aactgttagg acacactctc tatcgacgtc attattatcc gaaagaaaaa
    29521 aatttacgct ttgaaaagcg aatcgcttat cagaaatatg aagccatttc tcaaccaact
    29581 tttgataaac aggctcgtat ctacaataca caattatttt gataggtgga aaggaggatt
    29641 tttatgccat tgcttcatga cagaggagag cgaatcaaag ccaacgataa gcacttggtc
    29701 tatcattttg tactaatttc attgctggta gtttttctag tcgtcctact cccttttcat
    29761 tggagagcgt tacaatcggt gaactggcaa gcagttcagt tagatacatt tttctatcaa
    29821 cttcatacgc catacttttg gagcagtatt ctcactgctc tttttgtatg cggacttatc
    29881 agctggcttt attatcacta tcgtatagat tctataaaac agttagagca ccgtcaaaag
    29941 ttagctcgta tgattttaga aaatcattgg tatgaagccg aagaaactca atcagaggga
    30001 ttctttaaag atttacccag caagaaaacc aaacaacgaa tccgccattt tcctaaaata
    30061 tattatcgaa tgaaagatgg actaatccat attcatgtag aaatcacctt agggaaatat
    30121 caagatcagc ttttgcacct agaaaacaaa ctagaaacag gattgtattg tgaattagtg
    30181 gaaaaaacgt tgaaagattc ttatgtagag tatgtcctac tttatgatac tattggcaat
    30241 cgaattggca ttgaagacgt ggtagctcaa gatggacgtg tcaaattgat ggaatcggtc
    30301 tattgggagt tcgattctct ccctcacatg ttaattgctg ggggcactgg tagtggaaaa
    30361 tcctatttta ttctcacatt gattgaatct ctcctgcata caaatgcaaa gctttatatt
    30421 cttgacccta aaaatgctga tttggcagac ttaggggcgg tcatgaataa tgtctattac
    30481 cgtaaagaag atatgttggc atgtatcaat caattttacg aagatatgat tgatcgcagt
    30541 gaaaccatga aacaacaccc taactataaa acaggagaaa attatgctta tcttggtttg
    30601 tctgccaatt ttcttatttt tgatgaatat gtggcaatga tggatatgct aggtcgtgaa
    30661 agtaccaccg ttattcataa actcaaacaa attgtaatgt taggacgaca agcaggcttc
    30721 tttttaattc ttgcctgtca acgtccagac gcaaaatatt ttagtgatgg gattcgggac
    30781 aactttaact tccgtgtggc tttgggtcgt atgtctgaaa tgggctttgg tatgatgttc
    30841 ggaagcgata cacaaaaaca atttttctta aaacccatca agggaagagg ttacgtggat
    30901 acaggaaaaa gtgtgattag tgagttttat acaccccttg taccaaaaag gtacgatttc
    30961 ttaggagaaa taggaaaagt cattcaaaaa aagcaatctg agcctgtacc gcacgaagtg
    31021 aaaggtacag gttcagactt gctttgattg gtgtggcgtt agccacgcca atcctttagc
    31081 cccctctatc taacaggggg gcacaaatac aactgaaaac aggcaattct catcaaccaa
    31141 tgttgatggt acaaaggatt gcggaaaata tgaaaggtgt aaactcaagg gtcatagttt
    31201 acatcttttt tagattggag gtttgcgaat ggaaaacgaa aaaatttgga ttaaggaatt
    31261 gaaagaaaag cgactggctt atggcgtttc tcaaaataaa ctggcggttg cttctcatat
    31321 taccagacct tacctatcgg atattgaaac aggcaaggca gtccctactc aacaggtgaa
    31381 agaagattta ctgaacgctt tggaacgctt caatcccgat aacccattgg agatgttatt
    31441 cgactatgta cggattcgat tccctaccaa tgatgtggct caaattatcg gtgaggtttt
    31501 acgtttgaat atggattata tgctacacga agactttggc tattattctt atcccgaaca
    31561 ctatcgtttc ggtgatattg ttgtcctagt ctctcatgat gtaagtaaag gtgttttatt
    31621 agaactgaaa ggaaaaggct gtcggcaatt tgaaaacttt ctattggctc aacatcggag
    31681 ctggtatgat ttctttcaag attgcatgga acataaaggt attttcaaac gcttggattt
    31741 agctatcaac gataaaacag ggattttgaa tattccagat ttggcaaaga aatgtaaaca
    31801 ggaagaatgc atttccgtct ttcgaagctt taaaaattat cgcagtggtg aattggttca
    31861 ccgtgatgaa aaaccataca tgggaaacac actttatatt ggctcattaa aaagtgaagt
    31921 ctatttctgt atatatgaga aagattatga gcaatacgtg aaaaatgatg ttcccttaga
    31981 agacgcagaa gtaaagaatc ggtttgaaat tcggttaaaa aatgaccgag ccacccaagc
    32041 catgaaagat ttattggcac atcaacaggc ggaaaaaaca gcctttgaaa ttatcaatcg
    32101 ctatatccgc tttgcagata aagacgacac taaacgtcgg agtgattgga aaactaatga
    32161 acgttgggaa tggtttatcg gtaaaaatcg tggagcatta cgcctaacca cacagccaga
    32221 gccttattct tttgaacgaa cattgaactg gttacatcat caagtcgccc ctacattaaa
    32281 aatcgcaagc attttagatg ttctcaacgg tacaaccatt atctccacca tgattcagga
    32341 agccaaactg acagaaaaac atgagaaatt aattgaacaa caacatctag ccatggagga
    32401 tttgattaca tgagcctttt aacaaaagaa ttaaagaagt tagggtttca gtgtggcatt
    32461 gagtttcaag cgtatataca aaatacaggg aaatatacgt ctctcattat tgagggaaaa
    32521 cggcaagctg gtgataccat ctatacctat gatttttaca aggtgagatt ttataataat
    32581 tataccaatc gagtaaccgt ctatggagag catttaaccc cttttcaatt attaaggcgt
    32641 gtaaaaagct atatctatta tcgagaaaaa tatcttaagg aaaggaggac aatcacatga
    32701 attttgggca gaatttgtac aactggttct tggataatgc acagtcattg gtattgttag
    32761 cgattgttgt cattggactg tttttaggtt ttaaacgaga attttcaaaa ctcattggct
    32821 tcttgattat tgctctaatc gccgtcggac ttgtgttcaa tgcagctggc gtgaaagatg
    32881 tgttattaaa cttatttaac cgaatcattg gtgcttaaca gatatttttc atgataaaat
    32941 aggaagtata tattttgtta tgaaaggatt tatgaatatg tttacaaatg atcaatttaa
    33001 atctctttta attaaaacat tagaaagaaa gagctcttca aattattatg aaggaggtaa
    33061 cgagattgtt tctaaaatga aaatatcaga ggtaacttta gatgagactg acgattttac
    33121 ttattataaa ggatataaaa agggctggaa tacgctttgc acatatctta aaataagagt
    33181 tccttttgat gatttagact tttttgaaag tcataaagat ttgattaccc agacagcaag
    33241 ctccatatat gataaacaag gtgataatgt attagtggat actattttag taccacttcc
    33301 agagaattac gaagtaatta actttagtca gttgaaaatt agtgatgtgg tttcacaagc
    33361 tattgaagac gctgagtctt ttatgagtaa tggtgaatat caacgtgcct ttgacagagt
    33421 tcacactgct tttcatggat atttaattga aatactaaaa aaatatgaaa ttacagttcc
    33481 tagagacgaa aatctatcta aattatatag taggatacaa caattaattg aaaaagaaat
    33541 tcaacctact gaacttgctg atatagtgaa gacaacgata aggtcatcaa atggaatgat
    33601 tagctctctt aatgaagcaa gaaataggca ttcacttgca caccctaata caaatattat
    33661 tggaaagaga gaagcaaaac taataatagg tatttcaagt acagttacag attatattag
    33721 cggatacctt gataagtagc gtcattaact gattgctact ttttttatga ctatttttat
    33781 aaaaaggatg tgataagaat tggaaatgaa agtctatgtg gcaaatttag gacggtataa
    33841 tgaaggtgaa ttagtcggag catggtttac tccaccaatt gatgaggaag aaatggcaga
    33901 aagaatcggt ttaaatgagg actacgaaga atatgcaata catgattttg agcttccttt
    33961 tgatgtggac gaatacacgc cgattagtga aatcaaccgt ctatgtgaag ccattcaaga
    34021 aatcgaaggt acacctattt ataatgaact caaagaaatt caaggcatgt ggtttagtag
    34081 cttggaagaa ttactggaaa acaaagagga tattcattgt tatagtgatt gtgattcgat
    34141 ggaagatgtc gcccgttatt acgtggaaga aacaggacag cttggtgaag tcccttccaa
    34201 tttacaaaat tatattgact accaatcttt gggacgtgat atggaaatag agggtaatta
    34261 cctagttacc tctcatgggg tatttgaata ttgtcagtaa gtttaacaat ccgtgttata
    34321 attaaattaa taaatagcac ggagaggtat aaattatggt aaatgtattt gaaaatctta
    34381 aactattaaa actagacatg aaagataaag gctgggtgat agattccttt tactttcgtt
    34441 acaaacagca aaattatatt gtacttgtta agctatttga aaaagaggaa aaagttccag
    34501 aatatgctct gttaaaacta gaatttttaa aggaaaacga tttttcagat atgctagctg
    34561 tctatgctaa ttcggtaaaa ttatatacag acacgaaaac aataagagaa tattttggta
    34621 tcgaatacag tagtaacttg ggagatgttt tatttcaatt tagccaaact cttgctcgat
    34681 ttattccgac agaagttagt gagaagaaaa atgaagacca aaaagaagcc atgtgtttct
    34741 cattaagtca aagtgattca gaagatccca gaaaaaaata ttgtttttca gttagaagaa
    34801 atcctttaaa gggaaatggt gagctgggaa aacgaagtcc ttttaatgac aataaaacga
    34861 gattgtatag acccagctta tatgaacgat tagggtcaga taccaattta agtttcagat
    34921 attcaatgaa tccagatgat gaagaaactg atgaaggtat tattgcaaaa tggacaaaaa
    34981 ataaaattga atagttaaag cattgaaaga gcaatcacct attttgattg ttctttttct
    35041 atttttacgg aaaggacgtg aagacatgaa aaaaataaga agttacacca gtatttggtc
    35101 agtagaaaaa gtgatttatg cgattaatga tttacaactc cctttcccaa ttacctttac
    35161 gcaaatgaca tggtttatcg tttctttgtt tgtcgtcata ctctttggca gtttaccacc
    35221 actttctctg attgacggag cactactcaa atacttaggg attcccattg ttctcactgg
    35281 gttcatgtgc caaaagacct ttgacggaaa gaaaccctat ggatttctaa agtctatcat
    35341 tacctatgct ctacgcccta aagtaaccta cgctggcaaa caaatacgat taaaaaaaga
    35401 aacaatgaat gaatcgatta cgattgtaag gagtgaacaa ctagatggca tatccgatta
    35461 aatatatgga agataatctc gtatttaatc atgatggcga agtattcgct tattatgaat
    35521 taattcccta taattacagt tttttaagtt ccgatgaaaa aattcaagtt catgataatt
    35581 tctgtcaatt gattgcccaa aatcgtgatg gcaagattca tgctctgcaa ctagcaaccg
    35641 aatcaagtat tcgagccaca caggaacaat cgaaaaaaga aatcactgga cgattaaaag
    35701 agatagctta cgaaaaaata gatgaacaga ccgaagcgtt aatttctatg attggtgaca
    35761 atcaagtaga ctatcgcttt tttattggtt ttaaactcta cctcaatgaa caggaagtat
    35821 ccgtaaaaaa tgttgggaaa gaaattcaaa aagcatttgc ggaattcttt catggtgtga
    35881 atcacaaatt aatgggcgat tttgtttcta tgcctatggc ggaaatcgaa cgcttttcta
    35941 agatggaaca gttgttagaa agtaaattat ccagacgctt tcagattcgt aaattggaga
    36001 aagatgattt tggttatatt attgaacatc tttacgggca aacaggtgtc ccttttgatg
    36061 actacgacta ccatttaccg aaacgatacg aagataaaaa agtgttggta aagaaatacg
    36121 atattctcaa acccactcgc tgtttaatcg aagaaaatca acgctattta cggattgaac
    36181 aggaagatac cactacttat gtggcgtatt ttacaattaa tagtattgtt ggtgaattgg
    36241 atttccctaa tagtgaaatt ttttattacc agcaacagca gtttactttc cccgttgata
    36301 ccagtatgaa tgttgaaatt gtcgccaata aaaaagcctt atctaccgtt cgcaataaaa
    36361 agaaagaact gaaagattta gatagccacg catgggaaag cgacaacgaa accagtacta
    36421 atgtggtgga cgctttagaa tctgtcgatg aattagaaag taatttagac caaaccaaag
    36481 aatctatgta caaactaagc tatgtggtac gggtatcagc aaagaatttg gacgagctga
    36541 aacaacgttg tgatgaagta aaagattttt atgatgatct gtctatcaaa ttagtacgac
    36601 cttttggcga tatgctaggc ttgcataatg aatttattcc ctcaagcaag cgatatatca
    36661 atgactatat tcaatatgtc acgtcggatt ttttggctgg tcttggtttc ggtgctacac
    36721 aaatgattgg tgaaaattct ggtatttacg tcggttacaa tctcgataca ggcagaaata
    36781 tttatctcca acctagcctt gcaagtcaag gaattaaagg ctctgtaaca aatgcattag
    36841 cgtccgcttt tattggttca ttaggtggcg gaaaatcatt ctctaacaat atgctggtct
    36901 attattcagt actctttggc ggacaggcaa ccattgttga cccaaaagct gaacgtggtg
    36961 aatggaaaga aaccttacct gatattgccc atgaaatcaa tattgttaat ctcacgagtg
    37021 agaacgacaa taaagggtta cttgaccctt atgtgatttt aaaactcccg aaagattcag
    37081 aaagccttgc cattgatatt ctcacgttct taacaggtat ttccagccga gatagcgaga
    37141 agtttcctgt cctgcgtaaa gctattcgga gtgtaactca aagcaaacaa cgagggttgt
    37201 tattggtgat tgatgaattg cgaaaagaag atacgcccat cagcaatagt attgctgacc
    37261 atatcgaaag ctttgtcgat tatgattttg cccatctgtt attcagtgat ggcagtgtca
    37321 cccaatccat tagcttggaa aaacaactca atattattca agtggcagat ttggtcttac
    37381 ccgattccaa tagctctttt gaagaataca ctaccatgga attattaagt gtggcaatgc
    37441 tgattgtcat tagtaccttt gctttagact ttatccactc tgaccgttcc atttttaaaa
    37501 ttgttgacct tgatgaggct tggagttttt tacaagtggc acaaggaaaa gctctatcta
    37561 tgcgattggt tcgagcagga cgtgccatga atgcaggggt ctatttcgtt actcaaaacg
    37621 cggacgattt actagatgaa aagatgaaga ataatattgg cttgaaattt gcctttcgtt
    37681 ccactgacct agtggaaatc aaaaagacct tagaattttt tggtgtggac aaggaagatg
    37741 aaaacaatca gaaacgctta cgtgaattag aaaatggaca atgtttagtt tcagacatct
    37801 atggtcgtgt aggggttatg cagtttcacc cgatttttga agagttgctt catgcctttg
    37861 ataccagacc gcctgtgcga aatgaggtgg aagaatgaca caaaaacaaa agaaatttct
    37921 tcactatagt tggataacgc tcctttgtgt gggcgttatt ttacttttat taggcacgct
    37981 gggcaatgca gtacaagcga caggcttagt tgatgaaacc attgatacaa gcaatgaata
    38041 ctccaagtat ggtttgaatc aatatcagct tgattattat gtggacaata gctggggctg
    38101 gcttccatgg aattggagtg atggcatagg acaatcggtg atgtatggat tgtatgccat
    38161 tactaatttt atttggacaa tttctcttta tctatcgaac gccacaggtt atttggtgaa
    38221 agaagcctat gccttagatt tcattagtca aacatcccaa gcaattggcg aaaacatgca
    38281 gacgattgca ggcattacac cgaatggctt ttccagttca gggttttatg tcggtttcct
    38341 ccttcttttt attctcattg ttggaatcta tgttgcctat acaggattgg ttaagcgaga
    38401 aacgacgaaa gtgatacgag ctgttttaaa ctttgtcgtg gtgtttattc tatcggcttc
    38461 ctttattgcg tatgcacccg attatattgc gaaaatcaat gatttttcca aagatgtcag
    38521 taccgccagt ctgaatattg ggacaaaaat tgttcttcct aacacggata gtcaagggaa
    38581 agacagtgtg gacatgatac gtgacagctt attctctgtt caagtaaagc aaccatggct
    38641 attgcttcaa tatggctcca ctgatgtaga agcgttaggg gaagaacgtg tggaaagttt
    38701 attgtctacc agtccagata caaacaatgg tgaagataga gaaaatgtag tgattgaaga
    38761 aattgaagat catgacaata cctatttaac cctaccgaag acgatttcca gattaggaac
    38821 agtcttcttt ttgtttgtat tcaatattgg tatctcgatt tttgttttcc ttctaacagg
    38881 aatcatgatt ttttcgcaag tgctatttat tattttcgct atgtttttgc caatcagttt
    38941 cctattaagt atgttgccga gttttgatgg catgggcagg catgccatta ccaagttatt
    39001 taatgtgatt atgacaaggg ctggtattac cttgattatt accgttgcct ttagtatttc
    39061 cactatgctt tattcgctgt cagcaagttc tccctttttc atgattatgt ttttacaaat
    39121 tgtgaccttt gcagggattt attttaaact gggcgattta atgagcctat tcactttaca
    39181 aagcaacgac tctcaaaaca tgggcagaca agtgtttcgc agacctcgtc aaatgatgaa
    39241 tcgacaaacc agaagatttc aacgcagtat gagtaagatt tttcgtggaa aagcagggtc
    39301 aacgaagcaa aagacaatga caacaaagca gaatcaaacc aataaagcta accatactcg
    39361 tcaaaatgaa cggactacac caaagaaaga aacagcactt tccaatgtga aaaagcaaca
    39421 aggcggtatc gggcaacgat taggtgaaaa gacagcccat gtattggaca cgaaaaatcg
    39481 tctagtcaat aaaagaaagc aggcaaaaca acagatacag gcgacaccaa cccatgtcaa
    39541 atatgccctt catcaaggaa aagagaatgt cactcataat gtaacagact ttaaacaatc
    39601 tatggcagat accaaagctc aacgcaaaca acagcgtatg aaccagcagg agcaaaaacg
    39661 aaagaacatt gctcaaaagc gtttggagct ggataaagca aaagaaaaaa gacgaaaaga
    39721 aaacccatca tctcctgttc agaataacca gcagcatgtt cgcaagcgtc agcaaaccgt
    39781 cacgcctgtt ttgtcacaaa acactaaacg tgaaaatcat cagcgaccat tgaaaaaaga
    39841 gcaaaaacaa aagacaccac cgaagcaatt caatcagagg aagctaaata aaagaacgga
    39901 taagcgagga caacaaacat gaagctaaaa ataacagtga tttgtagtgt ggtcttctct
    39961 ctcttctttt ttctcttatt attcctagtc attttctttg cagatgatac ggacagtggc
    40021 gaaaacaaca aggattcttc tattccacaa ggcggtgtga ccgtttcacc agaagtgttg
    40081 gcacaccgtc cgttgattga aaaatatggt aaggaatatg ggattgaaga ctatgtttct
    40141 tatatcttag ccattatgca agtggaatcg ggcggtacag cagaagatgt tatgcaaagt
    40201 tccgaatctt tgggtttacc gcccaacagt ttgagtaccg aagagtccat caaacaaggg
    40261 gttaaatatt tcagtgaatt attaaccagt gcagaacaac aaggagtgga tatagattct
    40321 gttatccaat cttataatta tggcggtggc tttttaaatt atgtgaggag tcatgggaaa
    40381 aaatatacct atgaattagc cgaacagttt tctaaagaga agtcgggtgg tcaaaaagca
    40441 gactatccca atcctatagc catacctgtg aatggtggtt ggcgatataa ctatggaaat
    40501 cagttttatg ttcagttggt ttcacaatac ctaaccgaca cttccccaac agaatttgat
    40561 gatgaaaccg tgcaagtcat tatggacgaa gccttgaaat acgaagggtt tccctatgtc
    40621 tttggagggg cgtcgcctac cacctctttt gattgtagtg gcttaataca atgggtgtat
    40681 gacaaagctg gcatttctct ccctcgtgtc gcccaagatc agtatgacgc tacgcaagaa
    40741 atctctatgg aagaagccca agcaggcgac cttatcttct ttcattcaac ctataatgca
    40801 ggcacatatg tgactcatgt ggcgatatat ttagagggaa atcgctttta tcatgcaggc
    40861 gacccaatcg gttatggcga cttaagcagt cgttattggc aagaccatct gattggggct
    40921 agacgtgtta ttcataacta agaaaggaat gaatcaaatg attataaaaa tcgagcgaaa
    40981 agaaaagaag gagaaaccaa agaaatctaa aaaaccgagc aagcaaagaa aagttccgat
    41041 gattaaagtt ggcacgcata agaaattaac ctttgtttta tgggttttat tgattggtag
    41101 tgtcggattt ggaatctata aaaattttac tgccattgac actcatactg tccgtgaaac
    41161 agaaatcatt aaacagcaga ttgtcgatac caaccaagta gaaagttttg tgaaatcctt
    41221 tgcgaaagac tacttttcgt ggcaacaatc ccaagaagcc attgataaac gaaatgaaaa
    41281 attgacccat tatttgacgg aagaattgca ggtattaaat gaagaaatga ttcgtaagga
    41341 tattcctact agttctagtg tgaacgatat acaggtgtgg caggtttctc aagtgaatga
    41401 gaacaccttt gaggtactgt tttctgttga acaagttatt accgaagaca aagacaagga
    41461 aactatatcg tctagctttc atgtggttgt ccatatagat gaatcagata atatggttat
    41521 tatcaaaaat ccgacaatga gtaagaaacc tcaaaaatcg gactaccaac ccaaacagct
    41581 agaaagtgac catacagtag atacagaaac gatggacgaa atcactagct ttttagaaac
    41641 gttcttccag ctctatccta ccgccacaga aaaagaattg acttactatg taagtaatca
    41701 tgtgttacct atgattaaca aggaatatgt tttcgaggaa ttggtgaatc ctatctttac
    41761 cagaaaagat aaccaagtaa tcgtgaatgt cgctgtgaaa tatttagatc aagaaacaaa
    41821 ggcaacccaa atctcgcaat ttgagttgat attagaaaaa caagataatt ggaagattgt
    41881 aaaataacaa atattggtac atgattacag atactttgta atcatgtact ctttttgata
    41941 aaaaattgga gattccttta caaatatgct cttacgtgct attatttaag tgactattta
    42001 aaaggagtta ataaatatgc ggcaaggtat tcttaaataa actgtcaatt tgatagcggg
    42061 aacaaataat tagatgtcct tttttaggag ggcttagttt tttgtaccca gtttaagaat
    42121 acctttatca tgtgattcta aagtatccag agaatatctg tatgctttgt atacctatgg
    42181 ttatgcataa aaatcccagt gataaaag
//
LOCUS       DAZKSU010000016        40647 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000016 DAZKSU010000000
VERSION     DAZKSU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.016"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1928)
                     /locus_tag="ACG8Z3_000905"
     CDS             complement(45..1928)
                     /locus_tag="ACG8Z3_000905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0823243.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(2194..3069)
                     /locus_tag="ACG8Z3_000906"
     CDS             complement(2194..3069)
                     /locus_tag="ACG8Z3_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0823244.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            complement(3178..4947)
                     /gene="aspS"
                     /locus_tag="ACG8Z3_000907"
     CDS             complement(3178..4947)
                     /gene="aspS"
                     /locus_tag="ACG8Z3_000907"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0823245.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            complement(4962..6254)
                     /gene="hisS"
                     /locus_tag="ACG8Z3_000908"
     CDS             complement(4962..6254)
                     /gene="hisS"
                     /locus_tag="ACG8Z3_000908"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0823246.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSIPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            6648..7595
                     /locus_tag="ACG8Z3_000909"
     CDS             6648..7595
                     /locus_tag="ACG8Z3_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0823247.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            complement(7684..8943)
                     /locus_tag="ACG8Z3_000910"
     CDS             complement(7684..8943)
                     /locus_tag="ACG8Z3_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0823248.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(9114..9560)
                     /gene="dtd"
                     /locus_tag="ACG8Z3_000911"
     CDS             complement(9114..9560)
                     /gene="dtd"
                     /locus_tag="ACG8Z3_000911"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0823249.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(9571..11784)
                     /locus_tag="ACG8Z3_000912"
     CDS             complement(9571..11784)
                     /locus_tag="ACG8Z3_000912"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0823250.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(12017..12781)
                     /locus_tag="ACG8Z3_000913"
     CDS             complement(12017..12781)
                     /locus_tag="ACG8Z3_000913"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0823251.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(12782..13729)
                     /gene="prmA"
                     /locus_tag="ACG8Z3_000914"
     CDS             complement(12782..13729)
                     /gene="prmA"
                     /locus_tag="ACG8Z3_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0823252.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(13742..14230)
                     /locus_tag="ACG8Z3_000915"
     CDS             complement(13742..14230)
                     /locus_tag="ACG8Z3_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0823253.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(14196..14867)
                     /locus_tag="ACG8Z3_000916"
     CDS             complement(14196..14867)
                     /locus_tag="ACG8Z3_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0823254.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            15005..16291
                     /locus_tag="ACG8Z3_000917"
     CDS             15005..16291
                     /locus_tag="ACG8Z3_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0823255.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            16363..16557
                     /gene="ssrS"
                     /locus_tag="ACG8Z3_000918"
     ncRNA           16363..16557
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8Z3_000918"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            16635..16919
                     /locus_tag="ACG8Z3_000919"
     CDS             16635..16919
                     /locus_tag="ACG8Z3_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823256.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            16992..17471
                     /locus_tag="ACG8Z3_000920"
     CDS             16992..17471
                     /locus_tag="ACG8Z3_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0823257.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            17694..17837
                     /locus_tag="ACG8Z3_000921"
     CDS             17694..17837
                     /locus_tag="ACG8Z3_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0823258.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            18182..18310
                     /locus_tag="ACG8Z3_000922"
     CDS             18182..18310
                     /locus_tag="ACG8Z3_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823259.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            18415..19998
                     /gene="abc-f"
                     /locus_tag="ACG8Z3_000923"
     CDS             18415..19998
                     /gene="abc-f"
                     /locus_tag="ACG8Z3_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0823260.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20435..21619)
                     /locus_tag="ACG8Z3_000924"
     CDS             complement(20435..21619)
                     /locus_tag="ACG8Z3_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0823261.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(21644..22651)
                     /locus_tag="ACG8Z3_000925"
     CDS             complement(21644..22651)
                     /locus_tag="ACG8Z3_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0823262.1"
                     /translation="MLPEKMEQGFNQKLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(22823..23182)
                     /gene="comGG"
                     /locus_tag="ACG8Z3_000926"
     CDS             complement(22823..23182)
                     /gene="comGG"
                     /locus_tag="ACG8Z3_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0823263.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(23154..23579)
                     /gene="comGF"
                     /locus_tag="ACG8Z3_000927"
     CDS             complement(23154..23579)
                     /gene="comGF"
                     /locus_tag="ACG8Z3_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0823264.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            complement(23542..23889)
                     /locus_tag="ACG8Z3_000928"
     CDS             complement(23542..23889)
                     /locus_tag="ACG8Z3_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823265.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            complement(23861..24331)
                     /gene="comGD"
                     /locus_tag="ACG8Z3_000929"
     CDS             complement(23861..24331)
                     /gene="comGD"
                     /locus_tag="ACG8Z3_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0823266.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(24306..24584)
                     /gene="comGC"
                     /locus_tag="ACG8Z3_000930"
     CDS             complement(24306..24584)
                     /gene="comGC"
                     /locus_tag="ACG8Z3_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0823267.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(24581..25630)
                     /gene="comGB"
                     /locus_tag="ACG8Z3_000931"
     CDS             complement(24581..25630)
                     /gene="comGB"
                     /locus_tag="ACG8Z3_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0823268.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(25587..26516)
                     /gene="comGA"
                     /locus_tag="ACG8Z3_000932"
     CDS             complement(25587..26516)
                     /gene="comGA"
                     /locus_tag="ACG8Z3_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0823269.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            26648..28402
                     /gene="ade"
                     /locus_tag="ACG8Z3_000933"
     CDS             26648..28402
                     /gene="ade"
                     /locus_tag="ACG8Z3_000933"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0823270.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(28500..29162)
                     /gene="trmB"
                     /locus_tag="ACG8Z3_000934"
     CDS             complement(28500..29162)
                     /gene="trmB"
                     /locus_tag="ACG8Z3_000934"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0823271.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(29232..30014)
                     /locus_tag="ACG8Z3_000935"
     CDS             complement(29232..30014)
                     /locus_tag="ACG8Z3_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0823272.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(30218..31429)
                     /locus_tag="ACG8Z3_000936"
     CDS             complement(30218..31429)
                     /locus_tag="ACG8Z3_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0823273.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(31422..32159)
                     /locus_tag="ACG8Z3_000937"
     CDS             complement(31422..32159)
                     /locus_tag="ACG8Z3_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823274.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            32297..32731
                     /locus_tag="ACG8Z3_000938"
     CDS             32297..32731
                     /locus_tag="ACG8Z3_000938"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0823275.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            32724..33068
                     /locus_tag="ACG8Z3_000939"
     CDS             32724..33068
                     /locus_tag="ACG8Z3_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0823276.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            33248..34258
                     /locus_tag="ACG8Z3_000940"
     CDS             33248..34258
                     /locus_tag="ACG8Z3_000940"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0823277.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDYLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(34327..35271)
                     /locus_tag="ACG8Z3_000941"
     CDS             complement(34327..35271)
                     /locus_tag="ACG8Z3_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0823278.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(35255..37975)
                     /locus_tag="ACG8Z3_000942"
     CDS             complement(35255..37975)
                     /locus_tag="ACG8Z3_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0823279.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(37972..39192)
                     /locus_tag="ACG8Z3_000943"
     CDS             complement(37972..39192)
                     /locus_tag="ACG8Z3_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0823280.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(39473..40318)
                     /locus_tag="ACG8Z3_000944"
     CDS             complement(39473..40318)
                     /locus_tag="ACG8Z3_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0823281.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT        12033 a         8442 c         7090 g        13082 t
ORIGIN      
        1 ttcaggcaaa aacaaatgtt tttgcctgaa gttttttgta gctatcattc tgtcacgttt
       61 gtctcactgc tgttcgtact tgaacttcct cctagcaagg cttcgctgac taagtgaatg
      121 taatgtgtca tcccttcagg atttggatgg acttgatcat cataaaacca gtcactatgg
      181 tcattactgt agctgtacca atcaatcagc gtcatattgt cgtatttttt cgtcatctgt
      241 tcaagcatcg cattcacgtc attttgccag cgcttcgtcg gtactcggac attgacccag
      301 tatacttttc gatcaccgat tgcatccatc acactatcaa actgagcttc cgagaatgag
      361 ccattcgttc ctagtccgat cagaacagtg tctttcaaca aatcttgttc ctttaatttt
      421 tcaatgtacg gtacactggc atataattgt cgtccgacat ctccatctac cactactttt
      481 ggataaagtt cttgtagatc cgttgcagca tccaacataa ctgaatcacc aaatgccgtt
      541 agttctaatt tttgtgcctt attcccttgt gccttggaca actcgtactt atcaaggatc
      601 gcttggtcaa ccgaactagt ggtgttttct gaattgtttc tggtttcttc tgctaatttt
      661 ttactttcag cgatttttgc ctgcagttgt ttttgatcgg ctgttacagc gtttttcggt
      721 gccgtaacga aaccaacgag tgcaattgct acaagtaatg tgcctggaac aacccatggt
      781 ttgctcttac tgatgatcgg tttgctgaac catcctttca tcacttggaa tgtacgacca
      841 taatcaaaac gtgctagcgg acgctccaca tatcgataag aaagttcact gatgattaga
      901 atcaatgtga tttccactaa agtatgcaac aaaacatgat cggcaatatc agtcaccttt
      961 gcttcataaa aaatcataac tggaaactga tacagataaa tgccatagct tcgtttgcca
     1021 atccaagtaa atactggatt tgtcagccat cgattcaaac ttgctccggg gtgtgctgtc
     1081 actgcaacta gcactacgca taacagactc actaggagca ttcctccgta atagatgaaa
     1141 ttgtaatgat catctaaaaa gaagaagcaa agaatcagtg ccaaaagcga gattcctcca
     1201 gcgatattca gaatattttt cgcttgtttc gggatgtttt tctttaaacg cgtacttggc
     1261 caaacaaacg caagcgcact tcccaaccat atagaaaaca gacgagtgtc ggtaccgtaa
     1321 taaacacggg ttggatcgcc tcctggtgta tacaagatca tcatcaagat ggcggagagg
     1381 aaggatgtac ctaacacagt ataaaagatc cattttcttt tacgaacaaa taccatcaac
     1441 aatacaaaca agatcggcca aaccaaatag ttttgacctt ctactgctaa agaccagata
     1501 tgcgtaaacg gtgactcatt agcaaatcga tcgaaataag ataaaccgtt attgatttgc
     1561 caccagttgt tgacatacag taagctgctg accacaactc ctcgaagatt gtttaacagc
     1621 cctcgctgaa acaaagtgat ataagccgaa gctgtcacaa gcataaaaac aagtgccgga
     1681 tagagtctct tcattcttct gacataaaat tgccaaatat tgatttttcc attttgttcc
     1741 cattcctgcc gcagcagatc cgtgatcaaa taaccagata cgacgaaaaa aatcggaacg
     1801 cccaaatagc cgcctttcat actagttggc agaaggtgat agaagataac acccaccaca
     1861 gctaatgaac ggagcccatc aaacccagtg atgtagcggc tgttttttaa ccttttttgt
     1921 ttttccattc aatattccaa cttttcataa acttttttca ttcgtaatat acgggtatta
     1981 agtgaaaata gcaagtagaa tcgtgtcctt tctctactat ttttttctat catcccataa
     2041 atttaaaaaa ataaacatgc tttttttcaa ctttaagaaa atttcaaata aaaaagcagt
     2101 ttttctttgc tgattctact atggtagaaa aaaactgcag acaaacgaac gtctgtctac
     2161 agcagtattt catgaatttt tgtcaaactc gatctaaatg ccatcctcat agaaacgacc
     2221 aagtatctgg acgttttctg caatacttac aaaagcttgt ggatcagaag ctttcattgc
     2281 ttctctaagt agaggcagtt cgtatcgtgt aacgatcgtc atcaatactg tctgcttatc
     2341 atgcttataa gcaccttctg cttcattgat gatcgtgatc cctctacgca tacagttttg
     2401 gatttcattt actacttttt ctggatgttt cgttacgatc attacctgca tcttcttttg
     2461 tttagtataa acgacatcag ttacttttcc actgacgaaa atagacaacg tactgtaaaa
     2521 catatattgc cagccaaata gaattccggc aacaaaaacg atcaatgcat tgaagtagat
     2581 cgaaatagaa ccaatcgact ttcctgtttt tttacgaatc gtgatactca cgatatctag
     2641 accgcccgat gataggccgt ttctcaacgc tagcccaata cctagtccca tcacagcccc
     2701 cccaaacaac gcacagatga ttggatcggt agataggact gtttccggta aaatatggat
     2761 acaaagggag gtcaaagtga ctgtgataaa agtgaaaatt gtaaacttct tcccgatttt
     2821 taaccacgcc aatacaaaca aaggtatatt cagtaaatac aaagttgttg ataccggtac
     2881 ttcgaatcct atcagtgatt ttgacaaagt cgtcaaaatc tgagctagac cagtaatacc
     2941 acttgaataa atatgtcctg gctgataaaa gaaattcaat gcgactgatg cgatcaacgc
     3001 ataaacgata gataccgaaa gcttggtcgt gtaatcatga taagcccaat tttcgaatgt
     3061 ttttttcatg gaaaagcccc gctttatatg ttatggcttc attataaagt gtttttcaat
     3121 taaatcaagg ttgtcaaaag aagcgtattc gacaaatcct ttttaccttt ttatcggcta
     3181 ttcttctata ttcgttacat caatattcaa ttcgaacaat tgtaaaggcg aaaccatact
     3241 tggtgcatca ctcatcacat ctgctgcttt tccgttttta ggaaaggcaa tgacttcacg
     3301 aatattatct tctccggcta ataacattgc aaaacggtcc aatcctaatg caataccgcc
     3361 atgtggaggg aatccgtaat ctaaagctgt taacaagaaa ccaaattgtt cttccatttg
     3421 ttcttttgta aagcctagag tttctagtac tttttcttga agctcacgag tatgaatacg
     3481 gatagaacct ccgcctagtt cataaccgtt caagacgata tcataagctt ctgcatagac
     3541 ttttgcaggg tcttcagcca ataacgggat atcttcttct tttggcatcg tgaatggatg
     3601 gtgagcagag acatatcgac cttcttcttc tgaaaattca aattgtggcc aatcagttac
     3661 ccataagaaa ttgaatttcg attcatcaat tagtcctaat tcttttccta aacgtgtccg
     3721 gatagcacct aatgcagcag cgacgatttc acttttatct gcgccaaaca tcagcaagtc
     3781 acctggtttt gcatctgttg ctttaatgat tgcttctgtt gcttctccca tgaatttagc
     3841 aattggacct ttcaatccgt cttcttctac tttcagccat gcaagcccct tagctccgaa
     3901 ttgtcctaca tattgaccta actgatccat gtcttttcgt gagtagcgat ctgcagcgcc
     3961 tttagcattc aatgctttca cgacaccgcc gttttcaaga gccatttgga agactttgaa
     4021 ttctacttct ttgactgtat cgcttagatc gatcaattcc atatcaaaac gtgtatccgg
     4081 tttgtcgctg ccgtagcgag ccattgcttc atcataggtc atacgaggaa atggcaatgt
     4141 gacttcgata ccgcgaactt ctttcattac cttcgcaatc aatccctctg tgtaagtttg
     4201 gatttcttct gctgttaaga acgttgtttc aatatctact tgcgtaaatt caggctgacg
     4261 gtctccacgt aaatcttcat cgcggaaaca acgaacgatt tgataataac ggtcgaatcc
     4321 agcattcatc agcaattgtt taaacaattg aggagattgc ggcaacgcat agaaatgtcc
     4381 agcatggaca cgtgaaggaa ctaggtagtc tcttgcgcct tctggtgtag atttccctag
     4441 gtaaggtgtt tcgatatcga gaaagtcatt atcatctaaa taatgacgaa tcgcttttgt
     4501 tgtcgcattt cttaatttga tgtttttagt catttttgga cgacgcagat ccaaataacg
     4561 atatttcaaa cgtatttcgt cactgattgc ttgatcatct tcaatttgaa atggcggggt
     4621 ttttgcttga ttcaaaatca cgatttcact tgcaatcact tcgaattcac cagtggccat
     4681 tttaggattt actgcatttt ctccgcgtct tctaacttcc ccagtcactt cgatcacgta
     4741 ttcactgcga catttatccg cgatttccca tgcttctttg ttgatttcag gattaaagac
     4801 gatttgaacg attccttcac gatcacgtag gtcgataaag attactcccc caaggtctcg
     4861 tcttttttgt acccatcctt ttaatgtaac tgtttgttct aattgttgtt cggaaactaa
     4921 tccgcaatag atcgttcttt gtgccattta tttttttcct cctactcgaa taaagtcatt
     4981 tcatcataga cttcatcaaa atgttcatat atctcttcta atggaaaact tttttctttt
     5041 cggcttgcca ttgctttgac attaatcgtt tgactagcaa gctcatcttc accgatcacc
     5101 aatactaatt tggcattcgc tttatctgct gtcttaaatt gcgcttttgc tttacggttc
     5161 atgaagtcgc ggtcagctga aaaaccaaag tttcgaatcg cttggaccac tttcaatgct
     5221 tcgatatttg tttcgtctcc gagtgcgacc acgtaagcat ccaactcatt aaatgcaggg
     5281 ataactactt cctctgcctc catagtcaag agtaatcgct cgatacccat tgcaaatccg
     5341 aagccaggta ttgaaggacc acctagttct tccactagat tgtcataacg tccgcctgcg
     5401 caaatcgttg cttgtgcccc catttttggt gcgtcactca tgatttcaaa aatcgtatgt
     5461 gtgtaataat ctagtccgcg aaccatatta ctgtcgattt cataaggaac agccaatgca
     5521 tccagcatgg aaacaacggt atcaaaatgt ttttgagcag gctcgcttag ataatctaag
     5581 atagatggag cttctgccac aaatttttga tctcgtttgt ctttactgtc caatacgcgt
     5641 aatggatttt catgaaggcg gcgtttagag tcttcactca gctctgattc aaaaggtaaa
     5701 agatagtcga tcaatgcttg acgataggct tgacgcgttt ctttatcacc taatgaattg
     5761 atgactagcc ggatttgcgt gatccccaac tgtttgaaga aatcaagtgc catcaccatg
     5821 ctctccacat cagttgcagg gttttctgat ccaaatgctt ctactccgat ttgatggaat
     5881 tgacgtaacc gtcctttttg agggcgttcg tagcgaaaca ttggtcccat ataaaaagtt
     5941 ttgaacggtt tcgcatactc tgggccgaac aatttatgct caacaaaaga acgaacgatt
     6001 ggtgctgtac cttctggtcg aagcgtcaca tggcgttccc ctttgtcata aaaatcatac
     6061 atctcttttg aaacgatatc cgttgtatcc cctacacttc gggatatcac ttcatagtgt
     6121 tcaaaaatcg gtgtacgcat ttcattatac tgataatctc tgaataataa acgtgctgtt
     6181 tcttcgacaa actgccattt ttctgattcg cctggcaaaa tatcatttgt tccttttggt
     6241 ctttggaaac tcatagtttt ctctcccttt cttagctatg taaataaaaa aatcaccctt
     6301 gttcatcatg aacaagggtg aatgaaattc atacacggta ccacctaatt ttgaaagatt
     6361 tctctttcct cttacgaata acgctcgtac gcgggacggc ttttcactcg tccatctcca
     6421 gagtgtcttt aggctaatga aactttagat cattttcagc ctcgatgatc ctctcttgta
     6481 aagtttcaaa aacttactcg ctctttcatt gacattcgat attacttata acctactaaa
     6541 aaccagtctc aaagtcaaga gatatctgac tatttcccct cacaaaaacg ttttagttca
     6601 gctttttggt attttgtttt agactagaca aaaaggagga atcagctatg gcagaaataa
     6661 gaatcggaca ttcacaagtc tacgcagaac agcttggatt aggagcaaat gccgttggag
     6721 gacataatct ttttgacggt ttggaagatg agacaggaaa acaagtagtt cgtacagctt
     6781 tgaatagcgg gatcaatttg atcgataccg cttatgctta cggaaacggc cgttccgaag
     6841 aactgattgg cgaggtactg aaggaaaaag aatatgatcg ctcacgtgtc gtcatcgcta
     6901 caaaagctgc tcatgtacca aataaagggc gaacatttga taattctcct gagtttctca
     6961 aacaatcggt ggaagatgca ctaaaacgtc tgcaaactga ttacatcgac attttttata
     7021 tccactttcc ggatgaaagc acaccaaaaa atgaatcagt tgctacattg cacgaattaa
     7081 aagaagcagg aaaaatccga gcagtcggtg tgtcaaactt cacgttagaa caattgaaag
     7141 aagcgaatgc agatggatat gtcgatgtag tagaagataa atacagtttg atccatcggc
     7201 aggctgaaaa agaattattc ccttatcttg aaaaaaacaa aatcagtttt gttccctatt
     7261 ttcctcttgc ttccggtttg ctgacaggaa aatatgaact tggtgaggaa aaacaatttg
     7321 gtgaaggaga tcctcgaaaa agaaatccag atttccaagg agaacgtttc agagaaattt
     7381 taaccgctgt cgatgtactt cgccccattg ctaaaagata ccaagccact cctgcgcaac
     7441 ttgttttagc ttggtacatg aagaatccac gagtgtctgt agttatacct ggagctaagc
     7501 gtccagagca agtatctgac aatgtccaag ctttagattt gcatttatcc aatgaagatt
     7561 atcaaaccat tgatgaagca tttcgtggat tttgatcaat tgaccttatc aatttatagt
     7621 aatatgaaaa aaggaagatc agcactgttt gatcttcctt tttttcgata agtttatgct
     7681 aatctaattc ttgtaaagta gcgctcggct attcggataa gtcggaaact gattgaatgg
     7741 aatcgtccaa tcttgttcag gtacagagaa ctcaaactta tttactagct gatcggaaaa
     7801 cacacgtaga ctatgaagag tgatccattc tcctgcacag cgatgcattt gtcgaaaatt
     7861 tcccccgcct tgtgcaatca tctcatattc ttctttatag gaaatatctt ttgctttccc
     7921 tacataccgt ttgatcataa aggaatctgg cgcttcaact gttcgttcat catggtctgt
     7981 tccatataaa tctaaaataa cccaactgcc ttctggtata cgataaccat ctacttctac
     8041 ttctttcagc gaaatcgcag gtagcattgg gaagaaagga taatagcgtc tcatttcttg
     8101 aataaacgga tcttgtaacg tcgaaaagtc attttttaat tgctgataca aatctggtct
     8161 actgaaaagt gcatgtccca tcaatgccgc ccacacagtt agagcaaccg ttggacggat
     8221 gatgttcaat aattcaacag ctgccacttc cagaggtaaa agctgaccat ctaaatctgt
     8281 ggcattggca aaagcatata atgccacatt ttcttttcca gccacaggat tttgtcgagc
     8341 ttcttcaatc aagccttgcg cccatttttc tgattttttg cgattttcga caccttttat
     8401 gtgatcaatc ggagaagtaa acgttccgct gatcatagag atttgatatt ctgctaattt
     8461 ttcgacctca tctttagaaa tcgctgataa attgatacct gcccactcgc aaatagatgt
     8521 aaagaaaacc atcttactta aatcaaatag ttcaaattga ccatgctgtg cttcaagtgc
     8581 ttgtgtcaga ttcttatcta agatacgatg ataatcttcc attctttccg gtgtcattag
     8641 atccataaaa atatttttcc gatggtgatg agcagctcca tccaaagtct gtactccacc
     8701 ttgaccaaaa agtgtcttca atactaattt tggcatagca ccttttcgtt tgaaattcct
     8761 tggatcataa aatacttttg ctgctgatga tccgtagatc gtgatagctt ctttattgaa
     8821 aatctttgct ttgacgacgg gagcgtccgc ttcatggcgt aactcctcca acatgttgta
     8881 tcctttttga tacaattttt ttaaatctgt gattttgata tctactactg gtacttcttt
     8941 catcttcagc aacctcctat aaaaaataac ttgtataatt taagcttaac tttttttccg
     9001 actgctgaca aaagaagcgc tttttgcaga caaaaaagcc acttaagtta gagtttatca
     9061 gtctaactta agcgacgtgt ttcatagttt ttactagtga aaaccagatt actttatttg
     9121 tttttagaat caataatgat cgttacaggt ccatcattga cgagagaaac cgacatatct
     9181 gctccgaatt gtccggtagc gactgtgatg ccgcgacttc tgattccttc attgaatgat
     9241 tcgtataaag gaatagctgt ttctggtcgt gcggcttcca caaagcttgg tcgatttcct
     9301 tttttcgtat ccgcaaacag tgtgaattga gagatactta aaatttgtcc tcccacatct
     9361 tctacactaa tattcatttt tccttgttca tcttcaaaaa tacgcatttg tgcaattttt
     9421 tttaccagat agtccacatc tttttgcgta tcggtttcat gaatgcctaa taaaacaagt
     9481 aggccgtagc caatttttcc aatttcttgc tgatcgactg acacagcagc tttacttacc
     9541 cgttgaacaa ctgctctcat atctgtcctc ctatccattc gtccggcgta cactgtagac
     9601 atcaggtact gctttgattt tatccacgat actctttagg tgggataaat tctggatacc
     9661 aaccgtcaaa tggatcgtcg ccattttatc tttattcgtc cgtgcttcaa cgctgttgag
     9721 atttttcgtt agcgcattga ctgtttgaag tacatcattc aataagcctg aacgattata
     9781 gccgtaaatt tccaaatctg catcatactc ttttcgtgta ttcgatgtat cttcccattc
     9841 gacttcaatc aaacgttctg ctacatttgg tttgtcaggc tgaacattcg gacaatctcg
     9901 acgatgaatg gatatccctc tgcctttagt gatataacca acgatatcat caccaggaat
     9961 cggattacag cagcgactga tacgaattag taagttttct accccttgaa tgacaacgcc
    10021 accttcatga cgtactttca ttttgtcagg ttcttttttc ggctgattca tgatttcttc
    10081 tgcttcttgc ttttgctgct cgattttttg ttctttacgt tctttttcag tcagacgatt
    10141 ggccatcgtc aaggggctaa cttctccata ccctacagct gcatagagat catcttctgt
    10201 ttgataatta aaacgatcga gtgcttcttg cagtttgttc ttcgtcaaaa tatctttagg
    10261 cgtaaatcct agatctgtaa tacatttgac cacggattcg tggcctttga tcacattttc
    10321 ttctcgatct tgggctttga agaaacgttt gatcttattt cttgctttgc tagtagcaac
    10381 taatttcaac cagtcgcgac ttgggccaaa tgaatttgga gaagtcatga tctcaatgat
    10441 atctccgttt ttcaatttgt aatcaagctg caccattttg ccatttactt ttgcaccagt
    10501 ggttttgtta ccgatatctg tatggatact gtatgcaaag tccaatggtc cggatccttt
    10561 tggtaactca gtaacgtctc cttttggtgt gaagacgtag actttatcac taaagatatc
    10621 tccttttacg ccttccataa attcagaagc atcatagctt tcgtcttgaa gttcgagtat
    10681 ctcatggaac cagcttaatt gtttcgtcat accatctggt tctacttttt cattttttcc
    10741 ttctttatag gcccagtgtg cagcaacccc gaattcagcg atttcatgca tttcctgtgt
    10801 tcggatttga atttctaccg gatttcctgc cggaccgatc acggtagtat gcaaagattg
    10861 gtacatatta gcttttggca tggcaatata atccttaaat ctgccgggca ttggtttcca
    10921 ttttgtgtgg attgctccta atacagcata acaatccttg attgaatcta cgatcactcg
    10981 aatagctagc aagtcataaa tttcgttgaa ctgctttttc tgatctttca ttttacgata
    11041 gattgaataa atgtgttttg gccgtccata gatttctgca aaaatcccca gctcttctgt
    11101 cgctattcga atatcttcaa cagtaccact tacgtatttt tcacgttctt ctctcttcgt
    11161 ctgcatcaag tggacgatac ggtaatactg ttttggattt agataacgaa gtgctgtatc
    11221 ttctaattcc catttgatcc ggctgatccc tagacgatgt gcaagaggcg catagatttc
    11281 taaagtttcc tgagcgatcc ttctttgctt atcttctcgc aggtgcttca acgtacgcat
    11341 gttatgtagt ctatcagcta atttgaccat gataactcgt aaatcttgag ccattgctag
    11401 cagcatcttt cggtggtttt ctgctagctg ctcttcgtgg gatttatatt ttattttccc
    11461 taatttggtt acgccgtcca ctaacatagc aacgtcatcg ccaaattctt ctctcagatc
    11521 ttctaaagtg atttctgtat cttcaacaac atcatgtaag aagcctgtag ccactgtatg
    11581 gggatccatt tttaaatctg ctaatatacc tgcgacttgg ataggatgaa taatgtaggg
    11641 ttcgcctgat tttctaaact gtccgtcatg tgcagcagtc gcgtattcgc atgctttctc
    11701 aacaaatgct acatgttcgg gacccatgta ttgactaacg agctttatga caccgggtcc
    11761 cgttaaaatt tcttcttttg tcacaggaat cgatccccct ttcttttttc ggtaaaacgg
    11821 gaatcgggac aaatatctgt ttgataattg tcccaaaatc cgtgcagtta tacgtttttt
    11881 tacggaaatg ttcgcttttt aaaagctttt tcaaacgatt tttcgtatcc attgtataaa
    11941 taattgtttc tattatacaa attgttgact attttttcaa ttctttctgt cgatactctt
    12001 ttattaatga agaacgttag ctgaaagaag gattcattaa ttcccattga tagctagcag
    12061 cacttaataa ataaagaggt gctgtttctg tacgaaggat tcttggtcct aatccacaaa
    12121 gtatcccttt gtgttctaca aaataatcga cttcatcgcg ggataatcca ccttctggtc
    12181 caaagacagc taagattcga ctatctggtt ttgtttcact aagaattttg gctgcatttg
    12241 atttttcgcc ttgctttgct gattcctcat aagcaaccaa gaccacgtca taatcatcta
    12301 gccgctcaaa taaatcttgt ttcgtttcca ataactctac tgatggcacg atctgccgat
    12361 gggattgttc cgctgcctct tgagcaatct tttcaagccg ttgttgtcgt ttcatacgtt
    12421 tttttgcatc ccatttaacg acagaggctg atgccgggaa accaagaaaa gcttgcgctc
    12481 ctaattcagt ggatttctga acgatccaat ccagcttttc ccctttagga tagccactcg
    12541 ctatggtaat cgaacttggc agttcctttt gattttcttc ttttttaatt tctttcagat
    12601 aaacagcctc ttcagtaatc tccgtgattt ctgcaacaat ggctatttga tcattgaatg
    12661 ccaaaaaaac ttcatcctcg ggttccatcc tcataacccg tacaatgtgg tgatagtttt
    12721 ccccttctaa aacaaaatag tcttttgatt catatgattc ttttaaaaaa taccgctgca
    12781 tctattccac ctctggtttt ttcaaaataa ttgcatacca gtctttttgc tggaaaattt
    12841 gatccacgat aaacccttgt tctaacatgg catccagaac catttgcttc ttctcttcaa
    12901 tgattcctga aacgatcagc gtgcctgttt gcttcagtaa acgccaagca tctgggatca
    12961 tcaataagat gatatctgct aaaatgttcg caacgattac atcactttca atctcgattc
    13021 ctgtcaaaag atcgttagct gatacatgga catctttggc tatttcattc atatccatat
    13081 tttctttcgc cgaacgaacg gctacttcgt ctaaatcaaa ggcatagaca tcttttgcac
    13141 caagatattt tgctgcaata cttagcacgc ccgacccagt tcccacatct agcaatgttt
    13201 caccaccacg caagactgtt tctagtgctg tcaatgtcaa ggaagtcgtg ggatgagtgc
    13261 ctgtgccaaa tgccatacca ggatctagtg tgataatttt ttctagagga tcttctgtct
    13321 ggtagttttc ccagcttggc gcaaccgtca gcattcgcgt aatacgcacc ggatgatagt
    13381 atttcttcca ggctgttgcc cagttgcttt cctctacttc actaactgtt actttatttt
    13441 ttccaatagc tagaccaaac tcaggtaatt gtgtgatacg atctttgata aaaggcaaga
    13501 tttccggtaa gaaagtcgtt tccggaaaat aagccatgac tagtgcgcct tctttcagcg
    13561 atgtaaaatc atttttatct aaaatctcac catattgatc cgactgaaaa tgttcaacat
    13621 ctaaagaatc ttcaatcgcg acaccactcg cccctgcctc catcaaaata ttagcaactg
    13681 cttcgactgc ttcactttca gtttctactt ttacttcatt ccatttcata tctgtcactc
    13741 cttaataact tgggtacggt aaataagtat ctgcttcatt ttgctgtttt acttctacta
    13801 attctgctag ttgctgatta tcccagtgaa gctcgaatac agcttcgtca tcgtcttcat
    13861 ccaaaaaatc aagcaaatcg ctttgccctt gagctaatac ttcatttaga tattccacta
    13921 aagaatcagc tactgatttt gccaaccctt tttttccttc ataagggatt actgctagat
    13981 aattgcgttc atcaaaaaca gatttctgag gattaaaaag aagtacgccg tcttcaaatt
    14041 cgataattgg ttcttgcgaa acgatccctt ctgcatcatc aatctcaaat tgtgctttat
    14101 tttctgcaaa caagcgaaca acgatctcga tcgtatgatt ttttgtatcc caatctaaag
    14161 ctacatcata gtctgtcact tgtttttcaa ttatttcatc taaatatgtt aatagatcct
    14221 tctttttcat tttctttcct ccaaccccaa tctttctcga tttggttttt tctttgtaaa
    14281 gagatataag gatttcccga cacaacaaaa cgcaacggct tttctgtcca tacacctttg
    14341 tttgggatac cgattctagg caaagcattg atcttttttg gtgtcttttt tttctcaaaa
    14401 actaagcgta acgaactatc tgctatagac tgaccataaa gttcttgagg aattgctaat
    14461 gcttccacta attttcccgg accgttgctg atatctggac cagtttttcc gccacgattt
    14521 ttactcattt gatcaatcat tgcggcgggt tcgattgcac gtatcattac tccttgagga
    14581 atcccttctg gctgagtaat gatgttcaaa atccgatgag tatgcatcgt gtaaaggtag
    14641 atcgtacctg gtttttcata cattgcccgc acacgcggtg ttcttcttaa accatagcta
    14701 tgggcggcct cgtcttctgg acctaggtat gcttccgcgt ctacaatata tccagctaat
    14761 cttccttctg gtgtgtcatg ctccaaatac atccccaata aatcccgtgc cacttctggc
    14821 gtcgttttat tccaaaaaaa ttcatctatc ttttgtttat ccgtcatttt tctcacctac
    14881 tttatcatac acaatattag ttggataagc tatgaaaaca aaacaacaga aggctgttct
    14941 ttctaggcat cttgctaaat atgatataat taagggacgt aaaaacaaga aggaggaaaa
    15001 tcctatgcaa cagccattgg cttatcgcat gagacctcga acgatcgatg aagtcgttgg
    15061 tcagcagcat ctagtcgggg aaggaaaaat catacgccgc atggtcgatg ctcgcatgct
    15121 ctcttccatg attctatatg gacctcctgg tactggaaaa acgagtatcg caagtgcgat
    15181 cgccggctct actaattatg catttcgcat gctaaacgcc gctacagatt caaaaaagga
    15241 tctacaagtt gtagcggaag aagcaaaaat gagtggtaca gtgatcctct tattggatga
    15301 agtacaccga ttagataaaa caaaacagga ttttctgctt cctcatttag aaagtggccg
    15361 aatcatcttg atcggagcga ctactgagaa tccttatatt accatcaatc cagcgatccg
    15421 cagccgtaca cagatctttg aagtcaaacc attaacagaa caagacatcc agttggcagt
    15481 cgaacatgct ttaagggata aagaacgcgg ccttggacaa caagcaatcc agttagacga
    15541 agatgctctt cttcaccttt cgcgtgcaac gaacggcgat ttgcggagtg cattgaatgg
    15601 gctggaactg gctacactat cgactccttc agataaagaa ggaaggattc atcttacact
    15661 gtcgatcatt gaagaatgtg tccaaagaaa agctcttacc catgacaaaa atggagatgc
    15721 tcattatgac gttatttcgg cttttcaaaa atcgattcgt ggaagtgatg tggatgctgc
    15781 acttcattat ctcgcccgtt tagttgaggc aggtgatctg gcaagtattt gccggcgtct
    15841 gatggttatc ggttatgaag atataggttt aggtaatcct gctgctgcgg ctcgtacagt
    15901 gaacgctgta ttagctgctg aaaaattggg attgcctgag gcacgtatcc cactagcaga
    15961 cgtcgtcgtc gatctttgct tgtcgccaaa atccaattct gcctacatgg cattagatgc
    16021 agcacttgct gatattcgag agggcaaagc tggagatgtt cctgatcatt tgagagatag
    16081 ccattacaaa ggtgcgaaat cactcaaccg tggcgtgggt tatcagtatc ctcatcattt
    16141 tgatcaggct tgggtcaatc agcaatattt accagataaa ctgaagaatg ctcagtatta
    16201 tcagccaaaa gatacaggga aatatgaaca ggctctgggt caacagtatt accgtataaa
    16261 agagtggaaa aagcatcctc caaaaaacta gacaagaaaa cggttctgtt tctgcgagct
    16321 ctctcattgc tctttttaaa ttcatatgct actatagagg tgggaaatct gtgatgttcg
    16381 aattgtcctc tttgtgtgtt gaccgaacat ttttattgat atcttgggac ccgtcttgtg
    16441 tcactgctgg taacatgcct tatcatttgg tagttcgaag ctacgaccat gggacccacc
    16501 tgcttgaatt cgcgggatca atactacagg acaaacaacg gcattaacgg agattaccat
    16561 cttttaaaga ataaagcaat ttgctcttgg caaattgctt tttctatttg ttgcttattt
    16621 tggaggattt gttcatgatt aggattttaa tgattatcgc cactttagta ttattattcg
    16681 ttagctatta tttatttaat aaacaggata tcttttttgt attgatcaac aaaaatgata
    16741 aaaatcaagg attcctacaa ttttacggag ctgcatatgc tgttttaggc gtaatgggta
    16801 ttttaacagc ttttttcaat caacgcttta ttgccttggt ctttttactg atcgtaatta
    16861 ttgtttctgc caccttcagt atccgatttg caaaaaaaat tgctgaacca aagcagtaaa
    16921 taattctctt tttccctaaa gtgttctaca ataaaagagt acaatacgat tgacttgagg
    16981 agtgaatgct tatgttacaa caatacaaaa aaatcatggt tgccgtagat ggttctgatg
    17041 aagctgaatt agcttttaaa aaggcggtca atgtagctat ccgaaataac ggagaacttc
    17101 tattagcaca tgtcattgac acacgttctt tccaaaccgt ttcatccttt gatggaatgc
    17161 ttgcagaaca agctactgaa atggcaaagc aaacattggc agattatgaa agcaatgcga
    17221 agaaagctgg cttgaataat gtaacctctg tcgtagaata tggctcacca aaacaaatta
    17281 ttgccagaga aatcccagaa gataatcaag tagatttgat tatgttagga gccacaggat
    17341 tgaatgctgt agaacgttta tttattggtt ctgtttctga atatgttatt agaaacgcag
    17401 cttgcgatgt gcttgttgtc cgaactgatt tagaaaacca attgccagct aaagaccaca
    17461 ccaaagctta acacgtagac gacctgccaa ctatcggcag gtttttttgc acaaaaaacc
    17521 gtgataaaaa caaaagacgc ggcaagcact catcggtctt tataatgttt aaaataaagg
    17581 gaaaacagtc aatgagttca agtaaaataa aaaaaagtga acaccagggc gattagtcgc
    17641 cctggtgtgg tttaaaacga cctaagcatg ccaaagctta ttggttcgtc aatgtggaag
    17701 tttccgatga atctgatacg aaaaaatatg aagtatcgat ggcggatacc gattttatcg
    17761 ccaaactaaa attttatttc gggttcgggt accgcgagaa ggcaagtgct acattcggta
    17821 aatcattcaa gaattgatga aaggctaccg cttgccattg caaccacaac gatactcctg
    17881 aacgcgatgt tggtgagatt tccaaagaga agagctgttt aatgcaacgg tattaagcaa
    17941 atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa caccatatga tttcgcctga
    18001 acgctggttt agctcagaat acaatgatgt ggtccacgag acattaccct cagtcagata
    18061 aaaaaagctc tctgacagaa ttacacatcg ataaatatta agtaaatgtc agcgctagtt
    18121 gaactttctg acactttcag gtacactgga aaagacaact acacgaagga gggaaaatac
    18181 tatgatgcgc atgactaaaa taacaagtaa tgaaaattac caacgatacc atatcgcatt
    18241 tagccatgca attggtttta cagttgctgt tgacgggaca agtttgtcca aaacagttga
    18301 attcacgtaa aatcaatggc catctcaagg tatttttctc atgataatct gagtttaaat
    18361 aacacaggcc atggataacc atggcctttt tgagtggtct caaggagaga gatcatgatc
    18421 atacaagcaa tttctattaa aaaaacaatc ggcgggaatc cattatttga ggatttatcc
    18481 tgtcaaattg aagctggtga aaaaattgcc ttagtcggta tcaacggaac tggcaaatca
    18541 acctttttac aaatcttgac cggcagagaa ggtgtcgaca gcggtgttat cagtcgcaaa
    18601 aaaggcttaa agatcgggac ggtcgaacaa gaacttacag tttccgaagc gaccgtcaac
    18661 cactacttgc tccattctgc cgctgagata caggacctca aacagcaaat gagtcgctac
    18721 gaagcattga tgacagaacc agatacaaac ttagagaagt gtctggcccg ttacggtgag
    18781 ctgcagcatc gctttgaaga actaggcggt tacgtattag aagaccggat tgcagcgatc
    18841 ttgcaaggct tagggattcc acaccgtcag gaagcacggt taacagaact tagcggaggc
    18901 gaacgagtta aagtcgcttt agccaaaatt ttagcagccg atgccgattt acttttactc
    18961 gatgaaccga ccaatcattt agatcttaac agtattcgct ggttggaaaa ctatttacag
    19021 aatacaaaaa aatcggttct ggtcgtttct catgatcggc aatttctcga tcaagtcacc
    19081 acaaaaacat gggaactgga agaaggtgca ttgattgagt atcccggaaa ctacagccgt
    19141 tttcgagtct tgaaagaagc tcgcttggct gagctgacaa aaaattacga actgcagcaa
    19201 aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc gtcagtgggc acatgaagga
    19261 gacaatgagt ccttcttcaa aaaagccaaa gaactggaac gccgcctggc gaaactgaca
    19321 ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa gcttgtctaa tggtggaaaa
    19381 tctggcaaag aagttttcat cattcaaaac ttgcaccagc agtacgctga tcaagtacta
    19441 ttcaaagaca gcagttttgc agtctatcgt ggagatcatt tagccatcat cggcgacaat
    19501 ggtgcaggaa aatcaacctt actcaaatta ttactcggtg aggaaaagcc ttacggtggc
    19561 accatcaaat taggtagtag cttgcaaatc ggttatttgc cgcagcagct gcagtttgcc
    19621 gatcccgata gccgtttgct ggcttacgcc atcacattga ctggaaacga agaagtcgct
    19681 cgacggcaat tagccaaaag tggcttttat caaagcgatg tgggtaaacg gatcaaagat
    19741 ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac tctttttaga aaaaatcaac
    19801 gtcctgattt tggatgaacc aaccaatcac ctcgacagct atgctcgaga agaaattgaa
    19861 gaaatgctgc aagactatca aggaacgctc ctagcagtca gccatgaccg ctactttttg
    19921 cagcaacact tccaagaagc actcgttatt gatcaggagc agatcagccg ccagccgctc
    19981 ggccaactga ttgactagga cgttttttcg caacaaaaaa cttgattttc atacctgtgc
    20041 tttcacgatt caggcaaagt acaggtggaa aatcaagttt ttattattcg atcaatgaat
    20101 gtttcggatc ggttttctag ctaaacataa agcgaagaat aaagtcgaca gcccacgagg
    20161 ctgatctaaa aatgtttttt ctttttacat atgtaatgtc ccttataaag atgaatgaaa
    20221 ataaaatatt gggattttcc tgaaaatcaa taaataaaat gccagtcttt tcccttttga
    20281 ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa aaagaaggga aattgaagtt
    20341 aaattaccat tttgcggtct tctccgcaaa aaaaacacat gcaagaattc cctcttgcat
    20401 gcgtttcatc ttttatccta acgaatgctt tggcttagcc gcgcagacgc tccacatcac
    20461 gggcaatcat tagttcctca tctgttggga tcactaatac tcttactttt gattcttttg
    20521 tagagatatc tacttcttca ccacgcaatt cattcaacgc tggatcgatt tcacaaccaa
    20581 accaagacat acctttgatg atttcactac gtacatgtcc gtcattctcg ccgataccag
    20641 cagtaaagac aattgcatca acaccattca tcgttgtcac ataaccgccg atgtattttc
    20701 ggatgcggtc cacgaaaatg tcataagcta aacgaatatg ttctttttca tagttctttt
    20761 caagatcacg catatcacta gacaatcctg ttaggcctag cagaccagat ttcttgttca
    20821 aaacattgat catttcatca atatcaatct ctaatttttc cattaaatat gggagaacag
    20881 ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc agctaatggt gtgaatccca
    20941 tagaagtatc catggattta cctccatcga ctgctgtgat cgatacacca tttcctaagt
    21001 ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa taattttgcc gcacgttccg
    21061 acacataacg atgacttgtg ccatggaagc catattttct tactttgaaa tcttcataat
    21121 aagacaccgg taagctatat aaataatttt gctttggcat tgttgagtgg aaagatgtat
    21181 cgaatacagc cacactcaaa atgtcaggta aaatcttttt aaacgctttg atccccatta
    21241 agtttgcagg attatgcaaa ggagcaaatt ctgctaattc agcaatgcgg tccattactt
    21301 tctcatcgat cactactgaa tccccataag tttctcctcc gtgaacaaca cggtgcccta
    21361 ctccggtgat ttcatcataa gagcttaaaa tttgcagttc aatcaactga tctaacagca
    21421 tttttactgc tacttcgtgg ttttcgatat ccatgatttg ttcgaatttt tgattatcgc
    21481 cgtacttgat ggtaaaaata gaatcttgta aaccgatacg ttcgacgatc cctttcgcaa
    21541 ctacggtttc ttctggcatt tgatataatt gccatttcaa acttgaactc ccagcattga
    21601 ttgcaattgt tttagacata tgactctcct tctcgttgaa tgattacaga gcagatgatt
    21661 tccagttttt aaactcgttg aaaaattccg tcaccttctc tggttctttt aatgaagcaa
    21721 gtttcacaag caagacttct tttacttgtt gtgcttgtgg tccttttttc tgtaagatca
    21781 aaatactttt ttgcgattgt ttattacgga acaattcatc tggcaattga atcattcctt
    21841 gaagataacc ttcttcttta aaccattttt tgatttcttc actttgatcc gtttctaaaa
    21901 atccactcgg catcaaaaac aaaccaaaac catccggctt cacatatttc attgattgct
    21961 ctagcaataa atgatgtgca taactatggc cttcttcggt acttgttaaa aactcttttg
    22021 ctttttcgtc attcggataa taaccgattg gcaaatcgct gattgcaaaa tcgacaggat
    22081 ctattaataa ttcctgcaac ccgtcttgat gaaagtattg aacatttgct tgtgtcaaat
    22141 cacttgtaga agctgcgaca gccaacaatg tatcatcaat atccacacca attccttctg
    22201 tttggtaccc cgcattcgat aggtttaaaa gaactgttaa taacaagttg cccattcctg
    22261 ccgcaatatc cagaatctta accggtgctt ttttatttgt gtatagttgt tcaataagaa
    22321 aaacaaataa aaaaccgata ctatcaggag tgagttgatg gttcgcctgc aaatgttctg
    22381 tctggctgcc cttcaataaa agtaattgag acagtctgcg ccattcttct ggctcgaaag
    22441 aaagtttctt caactcttca taaagagctg tgatccgttg cgttgtctct tttgtcggca
    22501 ctccatccac cacacgtact tgataatcat ctattaaatt ttctgcattt tcgacatagg
    22561 cttctaaaaa ggaagttccc aatgcattct gtaaaagctg aatagcttcc agtttttgat
    22621 taaacccttg ttccattttt tctggtaaca tggtgcacct catttctaga tatattataa
    22681 caattttttc acctgtttaa ttctatcgaa caattgacaa aatttcaatc gttttcactg
    22741 ttttttattt gtgaaacggc aaacattttt ttattaaaaa cagtgttata acagaaaaga
    22801 gggcgttttc acgaacattc catcattctg tactagtatc tgtatcattt tttacttctt
    22861 ctgtgaaccg aaagataaac ggtgagactt ccacctctaa gaatagggac tgttgtaccc
    22921 actgatatgt tacagatcca gttgtgtagt aaaaaactcc ttccgaactt tcggcaagcg
    22981 tagactgttc cgataaagcc atctgcttca ttatttttgc tatataataa tttttgattc
    23041 gtatattaag atccattgac aaacggtagc tttctaatgc tgccatcaat aaaaacgtac
    23101 ataaaagaaa taaaagcaag acagaaaata aaatagagcc cttataacat tttctactga
    23161 tcgttttttg tccatatggc atatccatct tctccatttt caaagtgtac ataaaaagaa
    23221 attgtgtgtt ctttttccaa taattgaagc tcagtgatac ttgttaacat aggttgatgt
    23281 ccgccggaag ttcttctttt acgtatcatt gcctgatatc tttcgatgat atactgttta
    23341 tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga cattgtgact gctcttgagt
    23401 tcttcttcta attgtgctaa aaatattagc cactcttttt gatctttacg ctgcaaaaat
    23461 tcacttactt ggatggtttg ctgaatcaaa agattgaatg tgagcaatag actgctcaat
    23521 atcatcagtc caattagaca ttcaatcagc gtaaatgctg gaattacttg ttttttcaat
    23581 actgatcgct tctttctcca tattgacaaa cactttttcg attactccat tattatcgac
    23641 aatttggata tcgtatggga tcatttcttc ccttcgctct ggtcttttgt gatacatccg
    23701 ctgcgtcttg atatcttcat aaatcattcg aataaatagc agttcctccc cacttttctt
    23761 ttctgcctga agcaaaaaca gctggccaaa gctcactaag tagacacaga cagaagcgat
    23821 cataagcgct gtaagggact ccaacaaaac aaatcctgtt ttattcaatt ctttttgtat
    23881 agcgaccact ccctatctgg aattgatacg taatcgtccg ctctttttct tcccaataaa
    23941 attgataagc ttttagtgaa ctttcattcc cggttctagc agcaaaagta atagaagcat
    24001 ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc ccaatttatt ttatctggat
    24061 tcggaacatg aaaaatgatt tgattctctt cactattcca ataaaagcct gtttggatct
    24121 gattcacaat agcgattttt tgcgtagaat agattcgttt ttcaaattgg ccgaaaaatt
    24181 gatagactgc caattcattc ttccatgcag aaaatgcgag aactggaaaa gaaaggatcg
    24241 ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt atatcctgat gctaaaacct
    24301 taccttcaag gccgctgata gatttctgca tgttctcgtt tgatatagcc ttcattcaac
    24361 aattcctcca cagtaggaac tcttccatta ttttgaagcg catagagctc tttttgtgtg
    24421 tctaccaact ggatgatcgc ttcacgactt tcttggtcta catgattcct ataacgcgac
    24481 aaattaggga cgaacaacaa cactaataca ctgatgatca gtaagacgat tagcatttcg
    24541 atcaatgtaa aaccgctata ataagatatt tttctctttt tcatggcaaa acctcctcta
    24601 tgcttccgta aacaggtaat aaaacagcgg cataaactaa taaaatcaaa gccgctacgc
    24661 ccaaaaaaat gattggctga ataaaatgca tccagtgatg aagacgtttg aaaagctgtt
    24721 ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa acttcccttt gcctctccct
    24781 gtaaaacaat tttactgaat tcttctgata gaaaacgata attctggaac tgctcagcca
    24841 atgacatacc tgcctccaat ccttgaatca acttagaggc aagatgctga atcagacttt
    24901 cttgtttcgt ttcctttaag cagactatga tttgccttaa ttcgaaacct tcatgaaaaa
    24961 gctttccaat ctctagtgac aaataagccg attgatacaa ggaatatatc ggtccaatca
    25021 ataattgtct gcttatccat tcactctgat caacaggaga caacttcatc agacgatacc
    25081 ttactagtaa agccaataga gtaaccgcta ggatgaatag tagaacatac caatgaaaga
    25141 cttgtatcag ctgaataccc caatgctctt gcatgaccat tcctgaacca ataagctgag
    25201 gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa aaaggtgagc aatagcatcg
    25261 gatatgcaag cagctttttc aaatcattgc gaaatccttc aacaagccga aactgtttgg
    25321 caatttccaa caaagttgtc ggcagattcc catgacgttc agctaactcg atctgtacca
    25381 gttgctctgc tccatatcct atttgcgaaa gtacctcact aaacgcttgc ccttccttta
    25441 gctgatgttg gatatttgcc aaaataacag gtggaaacga acggatcgtg agtaataaat
    25501 aaagggattc ttgcagacta aaaccagata aaagcagttc tcccatggtt tgtgcaaact
    25561 ggttttgctg gcttcttgtt aatcctttag aatagttctg actgccaaat attttcctga
    25621 gcttcttcat aactttcctc ccatgatttt tttactgatt cttcctccat aaattgataa
    25681 ctccataaaa cagaaggatg gtgattggca tcttccaaaa gttcttgata aatgacacca
    25741 gctaaacatt cagatagctc ttcttttttc tttgtcagtt catggattct gaccaatgta
    25801 ccttcaagat tgcgtgcatg tacggttgca aaaacccggt gccctgttag tgctgctctt
    25861 accgcagcat gtgcagttga ttcatctctt atctcgccaa tgattagcaa gtctggacga
    25921 tgccgtaatg cgagtctcaa aagtgtatca taagtctgtg ctattttcgg attgatctgt
    25981 agctgcagaa aaaacggttc ttctatctct acaggatctt cgatagtaat cacttgcaat
    26041 ttttcttcca aagccaaccg atacattaga gaagtcttgc ctgatccgac tggtccgctg
    26101 aaaaggtata atccacgtct gacggttttc tgtaaaatag ttgcaaaatc tcgagaacta
    26161 aagcaacgat ggggtgtttc aaaagtaccg tataatagcc gaatcactaa actctctcgt
    26221 tgaaggtaat ctcctacaga agacaaccgc agacgaactt cctgtttttc taaagaataa
    26281 gtaaatgcac ctaactgagc ttttcttttt tctccgatat ccatctgtcc caaatactta
    26341 aatcttgaga tcagttgctg tccatctgcc gaagagactt tcttccagaa tgttcgtttt
    26401 tttccatagc gaaaatagat gtaaaatact tctgaagctg gcaatatata tacgtcttgg
    26461 gcatgtttgg caaaactttc acttatcatt tgatccgata atcgttttat ttccattatt
    26521 ctcccctcca caaaaaacat acgtcttttt gatctttttg tttcaaataa aatgaattta
    26581 caaaaaaata ttggcaaaaa ccgaatttat gttatactta taaaaattta tcttgggagg
    26641 cactccattg gaaacaacat taaggaaata tatcgaagta gcaagtaaaa aaaagcctgc
    26701 tgacctagtg atcaagcatg ctaagatcgt caacgtcttt acgaaagaaa tcatggaaaa
    26761 agatgtagca atctgcgaag gaatgatcgt aggaatcggt gaatacgaag gaaaaagcgt
    26821 acataatgct aatgggcaat atctagtgcc aggattcatc gatggccatg tgcatatcga
    26881 aagctcactt ttatcaccaa gcgaattttc aaaagtatcc ttgcttcatg gagttactac
    26941 tgtagtgaca gatccccatg aaataggaaa cattgctggg agtacaggtc ttgagtttat
    27001 gatagaagat gcaagacaaa cactcatgaa tatctttttt atgctacctt cttgtgtacc
    27061 tgtaacccct tttgagacca atggtgcaaa tttaaatgca aaaaaattag cccctttttt
    27121 acaaaaaccc gaagttttag gcttagctga agtcatgaac tatcaagcag tagcaaataa
    27181 tgaaacagac atcctagaaa agatccaatt gatgcaccag cataagaaaa aaattgacgg
    27241 gcatgcagct ggtatcggca tggaagagtt aaatgtttat cctgcagcag gtattcggac
    27301 ggatcatgaa gctacaactg caaaagaagc taaagaacgt ttggatcttg gtatgtattt
    27361 aatggttcga gaagggaccg ttgcaaaaga tctggcatcg ctactaccgg cgatcacacc
    27421 tgaaaattca agaagatgct tctttgtcac cgatgataaa ctgatcaatg atttagtaaa
    27481 agaaggatca atcgatcata tcgtccgtca agctattgca cttggaatcg atccattaca
    27541 agccattcag atggcaacat tgaatgcagc agaatgtttt gatttgaaaa cactaggtgc
    27601 gattgcccct gggtatcaag ctgatttctt cttaacagac gatcttacca agctgccaat
    27661 tcatgaagtt ttctataagg gaacattagt tgttcaatct gggcaactcc aagaccattt
    27721 atttgctgat cattcaaatt catatacgtg ttgtctgcca aaactaaacg ctcaaccatt
    27781 aaatccacat tcgttagagc tgcttctttc ttcttcacaa gcccatgtga tcgaaattat
    27841 cccgaatagt ctagtgacta aagatttggt agaaaaagtg gaccgacaag gcgaacgatt
    27901 cattccttca atcgaaaaag accaattaaa gattgcggtg atcgagcgtc accatcaaac
    27961 gggaaacatt ggattaggca tagtgaaagg atttcagctg aaacaaggag cgatagcgac
    28021 aacgattgcc catgactcgc ataatctagt tgtcgttggg acaaacgacg aggatatgct
    28081 gtatgctgct aatcttttga ttcaaaaagg tggcgggatg accgttgtca acaaacagca
    28141 ggaaatagct tgtgtttctc ttcctatagg cggaatcatg acacaggaac ctttttcgat
    28201 cgtctatgaa cagttgaatg aattaataac taaagcacat caactaggag cagctaaaac
    28261 ctttgatcct tttttgacac tttctttctt aacattatct gtcatacccg aattgaagat
    28321 caccgacaaa ggattatttt cattttctcg cttccaattg attccacctt gtggaaaaac
    28381 cgatgaaata aatacatttt aaatcaaata aatcccagaa aatacattac atctgtaatg
    28441 ttcattttct gggatttatt tatggatatt aatgttttta tgaaagcaga aagttagttt
    28501 cacagacttt tctttttggt cttatcttga aattgagctt ctactcgata aatacgttga
    28561 ccttttgaag aaaatttttc ttcatactcc gtcataatat tgccttcaaa ttgactttga
    28621 tgtaaatcta accatacctg tttgaggatc atgccatatt gagaaaaact tgccagagaa
    28681 tactcaaaca atccttgatt atctgttttg aaatggattt ctccatttgg acgaagaatc
    28741 tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc gtttttcatg acgttttttt
    28801 ggccatggat cggaaaaatt cagatagatt tgatctactt cgctgtcagc gaaatactct
    28861 gtcaacgctg atccatccac atgtagaagt ttcaggttag gaagcggttg ttcgatcaac
    28921 ttgtctagtg caatcgaaac aacactgacc tgcatttcta ctccaatata attgatttca
    28981 ggatgggctt ttgccattcc agtgatgaac tggcctttcc ccataccaat ctcaatgtgg
    29041 attggatgat cgttttcaaa caattcattc cattttccct tccacaatgt tggatcacta
    29101 attacaaaat tcgggtgagc cgctaacatt tccgctgctc ccggacgatt tcggacacgc
    29161 attatttatc tccattcctt tcctctaatt aaaaaggtct ggccaaatct ccgcagagtt
    29221 tgggccaaac ctcagccact gaaaattcgt tttaattgta aaatttcttc gttcatccgt
    29281 cgatgatctt cacgtttgca atatctggtg atttcttgga gcaaattcat ggccgcatac
    29341 caatgaaatt tttcaagcat ttcgtcagtc gggcgtatgc cataactgat cagccactga
    29401 ttccagctag acagtggcac ataatttcct aaaatcgttg caatatccac cgcagggtct
    29461 gcaaacatga ttgaatccca atcgactaga tataaataat ttttacatac taaccaatta
    29521 cgatggttta catctccgtg tactactgat aagttttcct tatctattga cgggatctgc
    29581 tcacgaagaa aacgatggac aatttgcaga aattgatttt tctggagttc ttccggtaat
    29641 tctttttcat atttttgcag catcttctcc ggcgtcatga cacgaccacc gatacgggtc
    29701 agcatggttt taagagaatt tgaatgatgt aaatggtaca aaacatcaat cacgtcgttt
    29761 ctttgaccaa tctcattagg agtcagcaag cgaccttcca gccactcttg tgctgtcaaa
    29821 gtatcgccat tccccgttcg ttttgtccaa acaagttttg gtgtaattcc ttcccttgaa
    29881 agagcagcca acatcggagt cgtgtttcgt ttaataaaga ctttgtcctc atcacgcatg
    29941 cctatgtatg tttttcctgt gtcgcctttg atgggacgta accgccactc ttggtctaac
    30001 tgaaactcca tcatgcattc tcccgtcctc ttactctttg ttttgcagta cttattattc
    30061 tagtcgcctg aacactacac gtcaagcaca tatacgttct ttttcattta attgccattc
    30121 aaaaaaccag cttttctcaa aaaaaataga acgaacagca ttcttactgt caacgctcta
    30181 tttttcattc tcatttttac cctacacagc tttgttttca gtctgacatc ttcttcaaac
    30241 gagttggtac atatagttta acaaagagca tagtaacaat caaataagcc gctagcggaa
    30301 cccatcgctg ctgcccatct aatacgactg cagcaatcaa accaaaaata accgacgcga
    30361 taattaatac ccatgtgagt agatactgaa tcgcctgctt cttctgattc ttatggattg
    30421 gataaagctg aacgagaacc atatagcgga actggttcgc taaaggtaac agttggaaac
    30481 cgatcaaata taaaaataag aaaccgataa tcagactaaa ataaaagtcg actactccgg
    30541 caatcaataa cccacctata attactaatc ggaaataaag accactaaaa tcagaacctc
    30601 ttgccaatct tcttgcaaac aagtaaagat aagtgttttt tgattctctt ttaatatgag
    30661 tcaatatagg atccaaatag cgtcttcttt tgatctttgc cgtaatttca ggaacatctg
    30721 tgaataaatt gataaattga tagatctgat gcaacctatg ttcttctaca tgcaccattt
    30781 tctcccaatc caatcctttt tcttgttgct tccacaaaaa ccagtaatat agacttgcct
    30841 gtactaatgc acctgctatt cctgcccata ttagccaata aaatgataga aacaaaaaga
    30901 tcagagaaga aaaaaaccag cctaatttcc aataacgaat aacttgttga tctagtcgaa
    30961 acaattctgc tctcttgatt tgcagatgac tgaatttcaa actccataat atgatcagat
    31021 agacgaaaaa catcgaaaag gattgctccg tactaaccac cactaatggc atggccaatc
    31081 cgcatgccaa tatcaaaaag atcaacggaa aaatgcaaga atagcgaaaa gctcgttcca
    31141 aataggtttg catttctttt tctttaggaa gtaaaaaaac aatatctgct gcctctgcaa
    31201 aagtagccat cttacccacg aataaacaga gcatccaaaa tacagagatt atgagccctc
    31261 caattgcaaa cggtggctgc agggttttca accaattaga ataatataat cccacaccgc
    31321 caatcaagaa aagacaaact aaaacaaaat gatcgttcaa tacataacgc atatatttca
    31381 tcattttctt ttgatgtctt gctaaacgtt tttgaaaaaa actactcatg tgcctgctcc
    31441 tttgttaaag caatatagat ttcgtccaaa gaagaatccg gcagctgaaa ttcttttctc
    31501 aacatctcaa gagaaccgtc tgctcgtatc tctccgttat gtaagaccac aaaacgatca
    31561 caatattttt ctgcagtagc taaaatatgc gtagacatca aaataccggc tccttctttt
    31621 ttcatttcta ccatcaattc aagcaatgca ttgatagcta gtggatccaa gcctaaaaat
    31681 ggttcgtcga tgatatataa agaaggctcg atcaaaaagg cgcataagat catcactttc
    31741 tgtttcattc cctttgaaaa attagctggg aaccagtcaa gacgattaga taaccgaaaa
    31801 gtctccaaca acttttctgc tcttttcatt gctatatcca taggtatatc gtaggccatc
    31861 gcagtgactt cgatatgttc tctcaatgtc aattcttcat ataaagaagg agtctctgga
    31921 atatacccaa tttgtttgcg atattcttcc gtatttttct ttaaggatag atgattgatt
    31981 tctatcgttc cttttgaagc ttccagcaga ccgataatgt gcttgatggt cgtacttttc
    32041 cctgctccgt tcagtccaat caaccctact atctctccag gctgtacatc gaaactgata
    32101 tcttttaaca cagggagatg tccgtacccc cctgtgacat gatcgataat taaactcatt
    32161 ctttttcctc cgccatttat ttttatttat tataccacga agaaaatgac ggtattttgt
    32221 ttttttcctt acaatagctt taacttatgg taaagttaag ctgaagtatt tattctttat
    32281 agaaaggatg ttttatatgg aaaactgtat tttttgcaaa atcatcaatc aagaaatccc
    32341 aagctataaa atatatgaag acgacaaggt ctatgctttt ttagacatct ctcaagcaac
    32401 gaaaggacac acattagttg tgcctaaacg tcatgtagca gatatttttg aatatgatcc
    32461 cgaacttgca ggagaggttt tttcgcgtgt gcctaagatt gctcaagcat tggaaaaagc
    32521 ttttccagaa atgaaaggtc ttaatatctt gaataacaac cgtgaactag cctatcaatc
    32581 tgtttttcat tcacatatcc atttagtccc tcgctacagc aaagaggatg atttttctat
    32641 tcactttgtc aatcatcaag atagttatgg atcggaagaa ctaaaagcta ttcaagaaac
    32701 aatcgtaaag caggtgtcct gcgatgatta aaaacttttt caaaggactg ttatttggag
    32761 ctgctgctgg cactgtcgga ggtctgttat tggcgcccag gagcgggaat gaaacaagaa
    32821 agaaactgat cgaagaattg gaagaagcaa caactctgac aaatgattta aataacagcc
    32881 tgaatcattt taaaagcgca ttgatcgaaa caaaagaaac agctgaatct gtgattcctg
    32941 ctttccaaga agcaatcaaa aaagatatcg aagattttaa attccaagcc gaaccacgta
    33001 tcacacaaat caatgaacaa gtagaaaaac taaaagctga tctgccagag tatcctgaac
    33061 aaatataata atgattattt tctcaacaga tttaactata taagtgagaa aagtttgcgt
    33121 atttcctagg aatgtgatga aattttgaat tttgtttgaa actaccacga caacgttttt
    33181 aaaggaaatg ggatatggta tagttaatat ataaaaataa taactaatta caggagtgca
    33241 aattttaatg aaaaaaaaat caattatttt ggcagctact agtgcgttgg ctgttttaac
    33301 tctagcagct tgttcaggcg atactaataa agatatcgca acgatgaaag gcggaacgat
    33361 cactgtatct gatttctatg atgaagcaaa acttgaatca tcgaaccaat ccttagtaca
    33421 acggatgatc atttataaag tattcaataa caaatatggc gataaagtaa ccgataagca
    33481 agtggatgca gaatacgaca aacaagcgaa atcattaggt gatacttttg aatcacaatt
    33541 agaagctgct ggatatacga aagatactta taaagaatat attcgtaaca atttagcttt
    33601 tgaagctggc ttgaaagctc acgtagatat tacagatgac tatttgaaaa ctgcttggaa
    33661 atctttccat ccagaagtag aagcacaaat catcaaatta tcaagtgaag atgaagcaaa
    33721 agatgtgaaa aaatcagcgg atgatggaga tgatttctct aaattagcca aagacaaatc
    33781 aactgataca gagacaaaag aagatggcgg taaagttaaa ttcgactcaa cgacaacaac
    33841 tattcctgca gaagtaaaag aagcagcttt caaattgaaa gacggtgaga tctccgatgt
    33901 tattactaca acaaatccta cttcttatgc gacagaatac tacgttgtca aaatggtgaa
    33961 aaaccaaaat aaaggcaatg acatggataa atacaaagat caactaaaag atatcgctac
    34021 tgaaactaaa ctaagtgaca atgcattcac aactaaagta atcggtgaag aattaaaaga
    34081 tgcaaatgta aaaatcaaag atgatgcctt tgaaaatgtg ttgagtgcat tcacaacaac
    34141 ttcatcaagt acgaaagact caagtgaaac tacagcttca acaaagagtt ctgacacaaa
    34201 atcaactgat tcaacaaaag aatcttcaac tgaagaaaca actgattcat caaaataaaa
    34261 gaaaacaaaa aaagcgaaga cttatgtctc gctttttttg ttttgaatga aaaaaataaa
    34321 tttttgctat aaatcttttg gtacatagaa acttcggttg tccataccga aaatccgatc
    34381 ggtgtatttt cctggttctg tttgacgaat cgaagtcaac atcatctgca tcgaagcatc
    34441 gatattgtcg atttgatgta atatttctgc ttccattatt cttggacgta caggtgagcc
    34501 atattctaat aagccatggt gtgctaaaac catatgacgt aatacgagaa catcttcgtc
    34561 agcttcgctg attttcaacg caagacaggc tttagttatt tcttcgtcaa tcaataccag
    34621 atgaccgatc aaatttcctg caagcgtata ttctgtcgac ataggaccgg aaagttcaat
    34681 cacctttcct aaatcatgca aaatgatacc agcatacaac aatgctcgat tcagctcagg
    34741 atattcatcg gcgattgatt tccctaagtg aagcattgtc aaagtatgat aagctaatcc
    34801 actggcaaat gcatgatggt ttctttttgc tgccggaaac tcaaaaaatt gtttttgata
    34861 tttattaagt agatagcgaa caatacgatt ccaatgcgca ttggtgatct caaacaacaa
    34921 ttgattgatt tcttcttcca tttcttcttt cttcaaaggg gctctttcca tgtataaact
    34981 aggctctcct ggctcatcta cccttgttaa gcgcatatga agaatcttta cttgagggtt
    35041 tccttgataa acctctcttt taccgtttag aaaaacgact gtgccggctg taaatttatt
    35101 gatttcctcc tccgaagcat cccagtattt accatcgata gtacctgaag tatcttggaa
    35161 agtaaaagca ataaattttt ttccgttttt agcgattctt acatctgcac ttttgatcag
    35221 gacaaattgt tcaaactgct catcgattac taaatcacga atttttttca ttatttgctc
    35281 cttctaattg ttttacgatt tggttcatct catggtaata gcttaccatt tctttatcag
    35341 aagaaaaaca aattatttga tacttttctc caaatttagc taaaagttca gctaattgat
    35401 actttcgctg atgatcataa tgaagccagc catcatcaat gatgatcggg catatcgaac
    35461 ggttagcttc taatgagagg taagcaaatc tcatcgccat aatcagctga tctttcgtac
    35521 ctgtagataa ctcatagatg ggaaattctg tatatccgtc tgttaaagtc agtgtatcat
    35581 ttttcagtaa tatcttctga taatttcctt ttgttaaaat acctaaataa tcacctgctg
    35641 ctcttaacaa ctggggtaat tgtctttcac tcatctctgt tgaaagatca cctaagaatg
    35701 tcccggcgat ttcaagtgct ccccattcct ccagtaattc ctgtaatttt gctttttgcc
    35761 ggctagcttg ttgataaagt tcgtccaatg tgccgtctct ttgcaactct tctacttgaa
    35821 gttggagtcg ttgccgattt tcacttgctt ctttttcttc ttgctggagt tgggattgct
    35881 tttctttcag ttggcgcagt cttacagata atttctcttt tgtcagctct tcaggaaata
    35941 gtggagtaag catttccgat aattcattgt atcttttcat ctgctgttcg gcttgtttgg
    36001 ctttttgtaa aaagacaggt acttccgaag gataagataa gcctgcttga gtcaattgtt
    36061 cgctgtattc ttgtatccat actttttgtt tctttttcaa ttgattgatt tcttgcttca
    36121 ataaagtatt ttgctgatag cttctggcaa atttcatctg ttccatctct tgtataaaac
    36181 gggaaagcaa taccattttt tctgaaattg tcttatcttg tatcggtaac cattctttaa
    36241 caaactcaaa tttttggtcg atttgtcgta gtttttcttg tatttcctgt tctcttttat
    36301 gtttccgctc aaatacgcca tataatttct gataatctat gacttcctgt ccatattctt
    36361 tcatgagttt gagcgtatcc atctttccta agtgccgatg atccgcaaaa cgatatactt
    36421 cttgctgaag aatctgtttt tcttctccag ttctcttttg tttttcttct gcttcagcta
    36481 attgagctaa ggctagatca agttgtccta gtttttcttg ccacattcct tttatctcag
    36541 agaaatcatt tttagtttta atttttggtt gccttttgta gaagaaatag ccaatcatgc
    36601 ctgcaccaat tagtaaaagg atcactttca agggcattgg tacaaatata cctgcaatga
    36661 ttattgctcc gccagccatc agcatatacg gtttagatga agagactggt gctatctctg
    36721 actgattttg taataatgct ggataggtct cttccaagtc attcacttct gcttcaatca
    36781 tctcttgctg ttctttagct atttggagac gcacagaaat ctggctttct agatcagcaa
    36841 tccttctttc ttcagccaga agatgcttcc attcttcttc atataaaaat tcttccggta
    36901 acggattttt ctgccaatgc cacctttgtt ccaaaatctt caattctgaa tttgtttctc
    36961 tagtctcaat ttgtaatctt tgccattctt cgtttaattg ttccgctgta aattgaagtt
    37021 gctggagatc ttttagttcc tgctcctgct ctaaataaaa ataatatctg gctgactgct
    37081 gatccatccc gctatgcttt tccagtgttg cttctaattc ttccaaacgc tcatttgttt
    37141 gctgataccg tttggagaaa gactccagat ctaaaagaag ttgctcatca ggaagacctg
    37201 tagtgctgtt ttttttgaga gattgccatt cttcatataa agaaaaatta agttcttgct
    37261 gtctggctaa tgaaaattgt ttgttcgcct cttttaattg tgccaataac tgctgctgtt
    37321 gcgactcgta ttcctgttct tgttggatca acttgccaaa ataagcttcc tgttcttgtt
    37381 tttgatgaat tttttcttgc agctctttcc attcatttaa tttttgattg attagtaatt
    37441 tttggccttt tttcttgaat aattgttgtg ctttctgata atattcttgc cgcttttgga
    37501 atacttgaga actgcccgtg atgcctaaag aaatcagcgc atcatgtagc tctttttctc
    37561 tcaaatgatc tagttccatc aactgctctt gctggaaagt aaatacctgc tggaataact
    37621 ctttcgtcaa aggtgcgatc agtttttcta gtaactcatc acttcccact tgatccccca
    37681 gccacacctt cgattttccg cgattttgct gcttgtagcg ctcgacagca aattcaccat
    37741 atacaggatg tttaagccat attttgccgc cataggctga gccgtctttt ggtgtataat
    37801 cctttttttt acggcctttt gaagggaaac caaataatat tgcttgaata aattgataaa
    37861 tcgttgattt tcctgcttcg tttcccccat agatcagctg attgcctgaa taaaagtcaa
    37921 aacttttttg acgccatttt ccaaaagcag caatctcgat ccttaaaatc ttcattcctg
    37981 atcctcctca aataaaaaat cttgattaag cagctgcttg gctttttcaa gtgtttcttc
    38041 ttgatattcc ggcagttcgc tcaatattct agccgcttct tgatgactat atacttcatt
    38101 taaaatttgt tggaaatctt gaggtgtctg atagtattga aataactgtt ccatcaattt
    38161 agcagaagca gcaagtgggg tcttcgtata aaaagtattc tctatgagag aaatacgcca
    38221 aataaagaaa tcatttatcc tatctgagaa ttcctctaac aaatagtcta atagctctcc
    38281 tgattgtatc cgttccaaaa catctgtatt gagatggtca taatctttta attttatctc
    38341 aattaaacta aatccatcct ctacttttga aagctgctga cgaatgtgaa caatgacctc
    38401 ttttgtcgtt cttgctttcc tcaatgatat ttcctttgtc ttccaataga cctctgctac
    38461 tttaatcgga tgtatttggc aacttccttt acttaattca actagtagaa tagatgtaga
    38521 agcttgttcc tttttagtat gaccttgagg cgcacctgga taaacaattg tttgcttttc
    38581 atttaagacc gttggcacat ggatatgccc taaagcccaa taatcgtatc ccttttcttg
    38641 catctttgag ggttggaaag gagcatagtt tcctttttga gagacgccag gttccccgtg
    38701 gtacaaccca atatggtaat ctgtgagttc tctagaagga aattccatca ctttgtcttt
    38761 ttgaatccac ggttgacgat aactaaatcc actgagagag acagtttctc cactttttat
    38821 cgttattttt tttgtctcta ccgtttcgga tgtaaataaa tggacatttt taggaaagga
    38881 gaaccaatac cgctcttttt gatagaaatc atgatttccg aatattataa atacaggtat
    38941 atggttcttt tctaaccgtt tcatctgttc tgaaaaatgt ttctgtattt ttaatgaagg
    39001 acgattttga tgaaaattat ctcctgcaag taaaacaaaa tcaacgttat ttattattgc
    39061 ctgttcaatg atatttgata aaacggtcag attcgtcttc aatagttttt cctgtacagt
    39121 tttatcaaga gttgtcagtc cttcaaatga ccgatccata tgtaaatctg ccgcatggat
    39181 aaatttcacc attaaaaatt tcctcctttt tttcttttat cataccattt tcaatgtttt
    39241 ttttcatcaa taaccgaaaa tttgttcgta ttatgcaaag aaattacgat aaatggccga
    39301 cttcaaaaaa taagtttatt cgaaaaaaag attgtgacat gatttttgtc acaatcctaa
    39361 atatctatgt tatattcgct tcgtttgaca ctgaagaagt actatttgta ctggaattta
    39421 tgctccattt ttccgtattt agaattctat gttctatact accttaatta gtttatatgg
    39481 taagaattct ttcaaatgct tgagatactc cgtctttatc atttgaaaca gtgacataat
    39541 cagctttttt catgatttct tgatgactat ttcccattgc aaaactaata tctgcacttt
    39601 caaacataga taagtcattt aaactatctc cgatcgcgat tctcaaaggt ttctttaaat
    39661 tgtagattga ttccaaacga tcaaacacca cacctttaga tacattttga ggtacgattt
    39721 caatatttac aggtgccgat tcagtaacag acacatttag catctttttc aaatggttaa
    39781 atatattagc tttcaattga cgattaggaa taaaaataaa aaatttgtaa atgctgatag
    39841 catccttttc tataatttcc tcaatattat tataagggat tgaacaaata gatttttcat
    39901 actcaagctc atagtttagc tctttgatag aaaattcact ccctacctcg tgaaaaattt
    39961 ttttcatttt atttttataa ttctgagatt cataacttcc gagatttgta tatattttgg
    40021 ttggaaattt cgataggaca gaatcaattt tttttacatc atttttatcg aaggaagact
    40081 caaatatctt tttttctcca tcaaatccaa cagaaccatt taaccctact gctggtatcc
    40141 ttaaaccaga ttttttgaga atattattaa tgtcttctac ttctcttcca gaacaaatga
    40201 atggaaaaca gtcttccggt aaatgtttca ttgttcttgc atttttttga ctaatgctct
    40261 ggtcagaatt caaaagagta ccgtctaaat ctataccaat tagtttcatt tcatccatgt
    40321 ctcctatacg ttgattataa attcactgct tattgtaagt ctaaatttct cacaaaacat
    40381 atactaatta tacctaattt aagtaagtac aaacatcatt ttaagaatag gaaaggacat
    40441 ttatatacta ttctctgtat ttttgattta taaataaata tgaattgaat ccaaatcagc
    40501 tatattacta ttttataata accaactata aaaaacgaag ttaggtgaaa aactaacttc
    40561 gatattcata aaagactcta ttagttcaac ttagcaaaag atttaaacaa aaaaagatga
    40621 actatttcta gttcatcttt ttgcctt
//
LOCUS       DAZKSU010000017        39579 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000017 DAZKSU010000000
VERSION     DAZKSU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.017"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            360..758
                     /locus_tag="ACG8Z3_000945"
     CDS             360..758
                     /locus_tag="ACG8Z3_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0823282.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            995..1537
                     /locus_tag="ACG8Z3_000946"
     CDS             995..1537
                     /locus_tag="ACG8Z3_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0823283.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1620..2171)
                     /gene="lepB"
                     /locus_tag="ACG8Z3_000947"
     CDS             complement(1620..2171)
                     /gene="lepB"
                     /locus_tag="ACG8Z3_000947"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0823284.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(2323..2550)
                     /locus_tag="ACG8Z3_000948"
     CDS             complement(2323..2550)
                     /locus_tag="ACG8Z3_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0823285.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(2547..3065)
                     /gene="msrA"
                     /locus_tag="ACG8Z3_000949"
     CDS             complement(2547..3065)
                     /gene="msrA"
                     /locus_tag="ACG8Z3_000949"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0823286.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(3085..3798)
                     /locus_tag="ACG8Z3_000950"
     CDS             complement(3085..3798)
                     /locus_tag="ACG8Z3_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0823287.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(3801..4697)
                     /locus_tag="ACG8Z3_000951"
     CDS             complement(3801..4697)
                     /locus_tag="ACG8Z3_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0823288.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(4855..5697)
                     /locus_tag="ACG8Z3_000952"
     CDS             complement(4855..5697)
                     /locus_tag="ACG8Z3_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0823289.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5829..6482
                     /locus_tag="ACG8Z3_000953"
     CDS             5829..6482
                     /locus_tag="ACG8Z3_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0823290.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(6545..7072)
                     /locus_tag="ACG8Z3_000954"
     CDS             complement(6545..7072)
                     /locus_tag="ACG8Z3_000954"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0823291.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(7092..8039)
                     /locus_tag="ACG8Z3_000955"
     CDS             complement(7092..8039)
                     /locus_tag="ACG8Z3_000955"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0823292.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(8051..9934)
                     /locus_tag="ACG8Z3_000956"
     CDS             complement(8051..9934)
                     /locus_tag="ACG8Z3_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0823293.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(10097..12175)
                     /locus_tag="ACG8Z3_000957"
     CDS             complement(10097..12175)
                     /locus_tag="ACG8Z3_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0823294.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(12188..12463)
                     /locus_tag="ACG8Z3_000958"
     CDS             complement(12188..12463)
                     /locus_tag="ACG8Z3_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823295.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(12825..13280)
                     /locus_tag="ACG8Z3_000959"
     CDS             complement(12825..13280)
                     /locus_tag="ACG8Z3_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823296.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(13434..14642)
                     /locus_tag="ACG8Z3_000960"
     CDS             complement(13434..14642)
                     /locus_tag="ACG8Z3_000960"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0823297.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(14754..15086)
                     /locus_tag="ACG8Z3_000961"
     CDS             complement(14754..15086)
                     /locus_tag="ACG8Z3_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0823298.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            15234..16109
                     /locus_tag="ACG8Z3_000962"
     CDS             15234..16109
                     /locus_tag="ACG8Z3_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0823299.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(16191..16772)
                     /locus_tag="ACG8Z3_000963"
     CDS             complement(16191..16772)
                     /locus_tag="ACG8Z3_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0823300.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(16778..18037)
                     /locus_tag="ACG8Z3_000964"
     CDS             complement(16778..18037)
                     /locus_tag="ACG8Z3_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0823301.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(18039..19085)
                     /locus_tag="ACG8Z3_000965"
     CDS             complement(18039..19085)
                     /locus_tag="ACG8Z3_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823302.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(19320..19595)
                     /locus_tag="ACG8Z3_000966"
     CDS             complement(19320..19595)
                     /locus_tag="ACG8Z3_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0823303.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(19838..21148)
                     /gene="der"
                     /locus_tag="ACG8Z3_000967"
     CDS             complement(19838..21148)
                     /gene="der"
                     /locus_tag="ACG8Z3_000967"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0823304.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(21330..22559)
                     /gene="rpsA"
                     /locus_tag="ACG8Z3_000968"
     CDS             complement(21330..22559)
                     /gene="rpsA"
                     /locus_tag="ACG8Z3_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0823305.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(22694..23374)
                     /gene="cmk"
                     /locus_tag="ACG8Z3_000969"
     CDS             complement(22694..23374)
                     /gene="cmk"
                     /locus_tag="ACG8Z3_000969"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0823306.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(23423..24058)
                     /locus_tag="ACG8Z3_000970"
     CDS             complement(23423..24058)
                     /locus_tag="ACG8Z3_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0823307.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTNTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(24124..25527)
                     /locus_tag="ACG8Z3_000971"
     CDS             complement(24124..25527)
                     /locus_tag="ACG8Z3_000971"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0823308.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(25514..26545)
                     /locus_tag="ACG8Z3_000972"
     CDS             complement(25514..26545)
                     /locus_tag="ACG8Z3_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823309.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            26576..26797
                     /locus_tag="ACG8Z3_000973"
     CDS             26576..26797
                     /locus_tag="ACG8Z3_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0823310.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(26962..27699)
                     /locus_tag="ACG8Z3_000974"
     CDS             complement(26962..27699)
                     /locus_tag="ACG8Z3_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0823311.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(27852..29192)
                     /locus_tag="ACG8Z3_000975"
     CDS             complement(27852..29192)
                     /locus_tag="ACG8Z3_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0823312.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(29429..30952)
                     /locus_tag="ACG8Z3_000976"
     CDS             complement(29429..30952)
                     /locus_tag="ACG8Z3_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0823313.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(31331..31942)
                     /locus_tag="ACG8Z3_000977"
     CDS             complement(31331..31942)
                     /locus_tag="ACG8Z3_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0823314.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(32065..32190)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(32272..32988)
                     /locus_tag="ACG8Z3_000978"
     CDS             complement(32272..32988)
                     /locus_tag="ACG8Z3_000978"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0823315.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(32994..33563)
                     /gene="scpB"
                     /locus_tag="ACG8Z3_000979"
     CDS             complement(32994..33563)
                     /gene="scpB"
                     /locus_tag="ACG8Z3_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0823316.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(33547..34317)
                     /locus_tag="ACG8Z3_000980"
     CDS             complement(33547..34317)
                     /locus_tag="ACG8Z3_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0823317.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(34314..34685)
                     /locus_tag="ACG8Z3_000981"
     CDS             complement(34314..34685)
                     /locus_tag="ACG8Z3_000981"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0823318.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(34704..35591)
                     /gene="xerD"
                     /locus_tag="ACG8Z3_000982"
     CDS             complement(34704..35591)
                     /gene="xerD"
                     /locus_tag="ACG8Z3_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0823319.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(35687..36159)
                     /locus_tag="ACG8Z3_000983"
                     /pseudo
     CDS             complement(35687..36159)
                     /locus_tag="ACG8Z3_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
     gene            complement(36264..37127)
                     /locus_tag="ACG8Z3_000984"
     CDS             complement(36264..37127)
                     /locus_tag="ACG8Z3_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0823320.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(37323..39422)
                     /locus_tag="ACG8Z3_000985"
     CDS             complement(37323..39422)
                     /locus_tag="ACG8Z3_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0823321.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        10933 a         8227 c         6711 g        13708 t
ORIGIN      
        1 aatgcgcagt tttcgcaaca gattgattta ttttgaaata agactctatc attacaagtt
       61 catccgttgt aagatgggta taggtcattt gtactcactc cttataattc tttggacgga
      121 actattttga gtgtagcaca aatgactttt ttagttgtct agcttaattt tacaatcggc
      181 gttataaaga aattccatct attagttaag tgaaagtgaa ccagtcctca ttctatcaca
      241 tcttcacttt tttgatgatg caaattttta ttttggtttc tatatatgaa tctaaatttt
      301 tgacagcgtt tcctaaataa gagatgatag acgaacaaaa cagaaaggag acgaaacaaa
      361 tgaatagtat ggtttttgtt aattttcctg ttgctgatgt caatcgttcc gttgcttttt
      421 atgaaaagct gggttttaaa aaaaatgaag aattttctac tgaagaagct ggttcaatgg
      481 tatgggatga cactttttgg ataatgcttc tctctcatga tttttataaa aaatttataa
      541 aagataaaga aatagctgat aataaaaaaa caagtggtgc tttgatttcg tttagtgttg
      601 aaaatgcgga tgaagtaaaa aaatttgctc aaattgcaaa agaaaatggt ggtactttcc
      661 acgaagtaga tatgggaatg cctgaagaag aaatgttttc attagaagtc caagatctag
      721 acggaaatac attagaaccc gtatggatga agatgtaagc agataactaa tctgacgttt
      781 tttataacaa aaaaacaaag gcggatgata cgataagtat tctccgcctc tatttttttt
      841 attcgtacaa tctctttttt acagtttata aaagcagtct tctcaccctt tttttaattg
      901 ttagatgcca gtgtatgtaa atcaccaatg tgtcatgaca cattggtgat tttatggtat
      961 ccttttatta ttaactgaca aaggagagaa tattttgaac actttgattg caaaagacgt
     1021 ttttagattg cccgaaatga atgcaagatt gttgtcgacc tctgctcttt tattagccct
     1081 tgatgtcatc ttatacaaga tagcgattgg tcctgcttat tttcaaatca cattcggatt
     1141 tttaagtatg gcgcttatgg gttatctata tggaccgatc tgggctggtt tgcttgaaat
     1201 gttgtcgaac actttaaatt tcacgatatt tggttcagga acgtttcaag ctgcttttct
     1261 tatcacggct ttccttggtg gatttattaa tggcttattt ttatatcata aaaagataaa
     1321 cctgccttcc gtcatcgctg cccaattatt gattatgatc ccagtcagcc tttttatcaa
     1381 ttcttggctg atgtctgttt tgtttggtgc agatttcaaa gtaatttttc taggtagatt
     1441 catcaggaat atgatccaaa tccctattca aataatggtt gtttattcat ttatcaaaac
     1501 tttagaggct agaaaagtgt ttaaacgagc tgtttgattt tacgtactaa ggtagtaaac
     1561 tgattttgga ttaaacaata aagcgtgcag tctcaacaga ctgcacgctt tattgtttct
     1621 cactggatcc attttatttc atcaagtgga taatagacaa atcttgcttt acctagaata
     1681 tcagttttat tgatcgtgcc aaacgaacga ctatcttttg aaactcttcg attatctcca
     1741 agtacaaaat aattatcttc cggaagcttg gattgtcccg ttagctcttg tagtgtaaag
     1801 tccgtcgtat aggatgcatt tgcatgatct gatttcaggt ttttagttag ataaggttcc
     1861 ttaactactt tcccattgat cttcaattga tcattttggt agctgacatt ttctcctgga
     1921 agaccgatta ctcgtttgat ataaatcgtg ccatctgcta attgaaaaac aacaatatca
     1981 aatcgtctga tctccgaaaa tttttccatc acaaccatat caccttgctt caacacgttt
     2041 tccattgaat tgccttccac tggtaccggg atgaataaaa atccccgcaa catgaacgca
     2101 aggaatacag aaacaagtaa gtatttgaat acaagccaga agcgattaat atagcgttgt
     2161 ttttttgtca atctctatca atccatccct ctttttttca ttctttcttt attataagaa
     2221 aaatatcaaa aaaatgcgac tagatagctg aaataaaaat aattctttca attaggctat
     2281 tggacaaaaa acttttttga tcataaagta caaaactatc tcttatatgt catgattatt
     2341 ttctaaatat ttttcccatg cttcgtcaaa gatatccatg gacggcaaat agtcgacatt
     2401 cagttccaga taattagaaa tttcttcgta gctagagctg tgcttaggaa attggatgtc
     2461 tttaccggct tctgtagcga aaacagataa agcatcatgt gtgccaccgc ctcgcagtgt
     2521 cattaaataa tgaaaaaagc ttcgattcac ccttcgttcc tccaattttc ttcgataaag
     2581 gcagcccggc gtgcatggtt tcttttaaaa ttttcaggat gctttttata atactcttga
     2641 tggtcttctt ctgctagata aaatggttgg gctggttcga tttttgtaac gattggctgt
     2701 tcgaaacgtc cactttcagc taactgtttc ttactttttt cagcaatgat ccgctgttct
     2761 tctgtcaaat aaaagatcac cgggcgataa ttgtcaccgc gatcctcgaa ttgtccgaaa
     2821 gcgtcagttg gatcagtttg ctgccagtaa agttctacta attgttcata tgaaatcaat
     2881 gttggatcat agataatctc cactgcttct gtatgtccag tcgtttggct ttttacttgt
     2941 tcataagtcg gatctggtac atgccctcca gtatatcctg acatcactgt atggatacca
     3001 ggaagcgtgt cgaaaggctg gatcatacac cagaagcagc ctcctgcaaa tattgctcgt
     3061 tccattatga ttccccctct aatactactc atccgatgat tttttatttt cttttgtctt
     3121 gctttcagta ctgctggtgc tagttttgtt ggtatttgag ttgtttgaag catcagaggt
     3181 atcttttggc aaatatagac taaaccgaat gtcatcattt actaagtcga ttttatctgc
     3241 tttaatatac atcccgtttt gcattcggaa ttgatccaac ctaagcatga tagtcagatc
     3301 atctgtattg acttcgatcc aatctggtaa tttatagttt ctcttgatca tattcatcac
     3361 atctttgatt ggcaaactca atgttccgat agaaagactt ctagctttta gttggacatt
     3421 tccgttttcc atcacatacg gatcaaaata taaatagaaa tcgataggaa aacctagtac
     3481 ttcgaaagta ccattgagta gtgcttcatt ttccaaataa aatttatatt caatatcact
     3541 atctttttga tactcactaa ggaaaaagct gatgatttga ttgacttttt ctttatttga
     3601 attgatcgtt aagacaggat ctccttgtct ttccgtaaga gctggtattt ctgggagttc
     3661 tggttcccga ttagcaaaaa tacggctgcc taaaaatacg gtcgaaccaa gtactacccc
     3721 aagaaggata aggaaggcga atttccagta atttgttttc tttgttttgt ttgaagatac
     3781 gtcagatctt gttttcatgt ttattgatca tcctccttct ctaaccattg gttttgtgtc
     3841 tcgatcagct gatctctcac tgcatttgcc atcaattgat aacctaaatt atttggatgg
     3901 aaatgatctt cttcatagag cacattattt ttgatgccat ttagattttc tgtcgtgcta
     3961 gttgtttgat ctgttccttc ttctgtgaca cctacttcct gattcaatcc ttgataaagc
     4021 aaatcattga ttgggataaa gtagcagttc ggcatttttt ttgtcaccgt ttctgttcct
     4081 ttgttccaat tgttaacaat tgtctgcatg tcagtgatct caggaaagtt caaataaaat
     4141 ggattgtaga tgcctaaaat gtaaataggt gcggttttat tgagggatcg aaattcttcc
     4201 aatagttctg tgacattttc ctgatagttt ttcagtggct ttttaaagga attgaccgtt
     4261 aacccgaata aattattttg gataactttc atcaaatcat taccgccaac agtcagtgtg
     4321 ataaaatcag ctgttttgat attgtttcga atttcttctt ttgtttttac tcgctttaag
     4381 atctggtcac ttcgttcacc atttacacca tagttttctg tttcaaccgc ggtcaaatta
     4441 taacggtctt ttagatcatt tgcgacaagg ggaacgaatc ctccttgctt ggtttgatct
     4501 cctactcctt cagtcagcga atctccgata gctatgtagt gaagtttttc ttttttattt
     4561 ggatcttctc tcacagtagt tactggtttc attagtgggc cagctttcgg tatagaagca
     4621 gctaaacctg catagatgat gatcgcaatt aataatggga acaaaacgag gatcaaatgt
     4681 cgtttcgtta atttcatttt ttcacctctt gtattcgatt ggttctaatg ttatcaaaaa
     4741 ttttatcaaa aaggtagtct ttatgaaaaa agaaaacaat tattttccta attttataaa
     4801 gaaaaagaga ataagctgca agcctttcag gctgagggct tatcctcttt tcgactaatc
     4861 tacataatac atgattgcaa acgcgtcttt accagtatgc gtagcgataa ccggattcgt
     4921 atgcagtaca gggatatgca tgtccgggaa taattcttgc aaaccctctt tgaattcttg
     4981 cgccaattct tttgcatccg catgtgaaat accgatttgt cgcacatttg gcaattcttt
     5041 cagttcattt ttcaaattat taaaccattt atcgaacgtt ttctttcctc gccccttagt
     5101 tacagggatt aattcagaat gggcgaaatt catcaccacg cgtatgttca aaacactaga
     5161 cagcaaacca gttgttcggc tgattcttcc accttttacc aagttatcta gtgtggatat
     5221 tccgatgtat aattttgtat tgtcttgtac ttttttgact tcttcaagga ttttctctac
     5281 tgttttacct tcttttgcta attcagctgc tcgaattacc tgaaaagata atccttggtc
     5341 agtggtatca ctgtcgataa ccgttacttt cgtttttgtt agattactgg cttgtcttgc
     5401 tgcttctact gttccactca agccttttgt catatggatc gaaatcactt cactgccgtc
     5461 ttctcctagc tcatcataca tcgagatgaa ttctccaatt ggtggttggc ttgttttggg
     5521 cagatttttt gctttcgcca tcatttccat aaatctttcg ccgtctagtt ggtcatcatc
     5581 gggatatact gttccgtcga tcatgatgga tagcggcatg acacgaatgc ctaaagcatc
     5641 gcgtacattt ttttccatcg tacatgaaga atctgttacg atttttacat ttgtcattat
     5701 tttctctctc tctttcttta acaagatgcc ttattcatgt taaactacat gtaagacaat
     5761 ttctattttt agtataacag acaaataatg atttttcatt aattacacct agtaaaggag
     5821 ccgttttcat ggaaaaaacg aaatttaatc gaacgtatga aatattaaat gaagtgttta
     5881 atgcagtcac ccatggaatt gggactggat tgagtatcgc gggactagtg gttcttttag
     5941 taaaaggtgc ccacttgcat tcctccctcc atattgtctc ttatgctatc tatggatcga
     6001 tgatgattct tttattttta acgtctactt tgtttcattc attgattttt acaaaagcaa
     6061 aaaaggtgtt ccaagtattc gatcatagtt cgatttttct tttgattgca ggaagttata
     6121 ctcctttttg tttgctgagt atccgaggat ggctaggttg gactttattt gtattaatct
     6181 ggttgtttgc gattcttggc attatatata aagcgttgac gttgcataaa cgggagagag
     6241 tatcaaaagt atctacgatc atctatattt ttatggggtg gctctgtgtc gtggctgctg
     6301 ttcctcttta tcactctctt ggaatgactg gtgttatttt gatggtagca ggcgggcttt
     6361 cttacacgat aggcgcattc ttttatagct tgaattctat ccgctatatg catgttgttt
     6421 ggcatttgtt tgttatgtta ggtgcaggtt ttatgtactt ctctgtttta ttctttactt
     6481 aataaaaaaa ataaagctat ggatgggaca gctctaaata acagctagtc tatccatagc
     6541 ttttttatat cgtcaattcg cctgtcccgc tttttacagg attctcatta cgctgatacg
     6601 tttcaaattg atgcggataa cgattttttt catccatttc tccttgacta gtattgatca
     6661 gtgaccaatc agaccagtca acaggtggaa aataagcatc tccatcgaat acatgatgga
     6721 tgactgtacg gtataatatc gtacaaaatg gcagaaattc ttgataaaca gctgaccctc
     6781 cagcgatgaa aaagataccg tctgtttctt tttccagagc aagcacgtct tccacactat
     6841 gcatgacatg cacgccttct gctttatagg aagaatctct cgtcaaaaca atcgtctgac
     6901 gattcggcaa cggacggcta cccatccctt cgaatgtttt tcgtcccatc actagtgtat
     6961 ttgcttctgt catttgtttg aagaatttca gatcatttgg caaacgccaa ggaagacggt
     7021 cttcttttcc aatcaatccg ttttcatcct gtgcccaaat tgcagctaac attttctcat
     7081 cctttctgat tctaaacagc gataggagct ttgatcgccg gatgtgggtc atatccttct
     7141 aattggatat cttccatctc aaaatcaaat actgactgct tttcctgatt caaaactaaa
     7201 gtcggaaatg cgcgtatttc tcggctcaat tgttctttca tttgttcgat atgattgata
     7261 taaagatgag catctcctaa tgtgtgaaca aattcgccaa ccgctaaacc tgtttcatgt
     7321 gcaattaggt gagtcagcaa cgcataactt gcaatgttga acggaacgcc tagaaacaca
     7381 tccccactgc gttgatacaa ttggcagctt agccgtccct cttgaacata aaattgaaac
     7441 atcgtatgac aaggaggcaa agccatgcta ggaacatctt ccggattcca tgcagaaacg
     7501 atcaatcttc tagaatcagg agtctttttg attgcttcga tcacttgttt gatttgatcg
     7561 ataaagccac cgtcttttgt ttcccagtga cgccattgat agccgtagat gtgtccaaga
     7621 tcgccgtatg tttcagcaaa ggaatcatcg gttaggatct tctcgcaaaa aagcttgctc
     7681 tgctttttat attcttcatt gaattcagga tctgtcaaac atctatggcc aaaatcagtc
     7741 atatctggtc cattatagtc tggacttttg atgtagcgtt caaatgccca ttcatcccaa
     7801 atatggtttt tattttgtaa tagatagcgg atattcgtat ctccttttaa aaaccaaagc
     7861 aattcgcttt tgatcaatcc aaaaggtact cgtttcgttg ttaacaaagg aaaccctttt
     7921 tgcagatcaa accgcatttg gtatccgaat aagcttaatg tgcctgtgcc tgttctgtct
     7981 tctttacgat gcccttcttc caacaatttt cttcctaagt caagatacgc ttgttccatc
     8041 ctttttcctc ctaatcttct acccattcac tcaagtattc ccaccgtgcc attttttctt
     8101 caagttgtgc ttctgtttca gatacgtcgt tttgcaattc ttgtaatcga gtgaaattat
     8161 ctccttgatg attcatctct tcttggagca atgagatttt ttcttcaagt tctgcaatct
     8221 catcttcaat cgtttcccat tctttttgtt ccatgtaaga taatttcttc ttttcagcag
     8281 gttccttgct ctgtattttt ggtttcgctt tttcagcagg ctgctgggtc tctttttgct
     8341 tcagtaaata actggacatg tttccgtaat aaagttcttg ttttccgttt ccttggaata
     8401 ctaataactt atctgctacc ttgtccaaga aataacggtc atgggaaaca gtgatcacag
     8461 cacctggaaa acttctaaaa taatcttcca aaatcgtcaa tgttgctatg tccaaatcat
     8521 tggtcggttc atccaataat aaaacattag gttgctgaat cagcagtttc aacaaaaata
     8581 accggcgttt ttctccgcct gaaagtttgc ctatcagtgt cccatgcata aatcttggaa
     8641 ataaaaatcg ttcaagtaat tctgctactc cgatttgact gccatctgct gtttttgctt
     8701 cttcagcagc ttcttgtaag taagcaatca tccgtttatc tggtgccatt tcttcatttt
     8761 cttgtgtata atacgcaaga tgaaccgttt caccgataga cattgtaccg ctatccaggg
     8821 gaattctgcc agctagaata ttaagcaatg tcgattttcc cgcaccattt ttaccagtaa
     8881 tacctagtct ttcccgagat tgaatcaata aatctaaatg ttctagcagt gtttggtcat
     8941 ttatacggta actgccatct ttgatttcca gtactttttt ccctaaacgt tgtgtcgcta
     9001 ggttcagctc taaatcatct tcttggttta cttggtacaa gttctccttt agatcttcga
     9061 aacggttgat ccgtgcttgc tgcttggtgc cacgtgcctg tacgccagcc ctcatccaag
     9121 ctaattcctg tttataaagc cgcttgcgct tttcttcttg ttctacttct acacgatcac
     9181 gttctgcttt ttctaagacg taagcttcat aatttccttt atactcgtat aattttccaa
     9241 atgaaagttc gaagattcgg tttgctactc tatctagaaa ataacgatcg tgggtaacca
     9301 tcaatagtgc gccacggtat tgtttcaaat agttttccaa ccactcgatc gcttcataat
     9361 ctaaatggtt tgttggttcg tctagtaata gtaaatcggg ttcatcaatc aacacttgcg
     9421 ccagactaac tcgttttttc tgaccacctg ataaagtact gattttttta tctaattcac
     9481 tgattcctaa tttttgcaaa ataatctttg cgttggtatc tgtagtccaa gcatcttctt
     9541 tattcatttg ttcttctgct ttagcataac gtttttgcac atcagggtct tctccgtttt
     9601 cccctagttc gatcaatgct tcctcatatg ctcgaacagt ttggatcaag gggctgtttc
     9661 cttgaaagac tgcttggaga acagttaatt catcggaaaa ttcagctgct tgagaaagat
     9721 aaccaatacg gtaatcagtt ggataaaaca ctgtttgccg atcaccgtcg ctgctatcga
     9781 taccggcaat aatatttaag agacttgttt tacccgtccc gttcgtccca atcaacccga
     9841 tacggtcttt ttcgtggacc aaaaaagaaa tctggtcaaa caaatctttt tcaccataag
     9901 tttttttgag gtcagttact tttaattctt tcaacatgtt ccctccttat cttggatatt
     9961 gtatcataaa aatagtgaac ttcaggaaaa tttttttggt atttgttctt tttgtgttct
    10021 caaaaaaaga acaggaccga ttcgatcctg ttctgatatg ttttgttttt tataaaactt
    10081 gaaaattttt acctttttat tttattggac tcgttttttt tgactttgct cgaaacgcaa
    10141 atcggaaaga aaagttatca gatggtttgt tcatcgtata agattctaac aaattgatat
    10201 tcctgcgata gctgttcgca gaaaacgtcg tgattttttc agcaacttcg tattcatcaa
    10261 tgatttttct ttcaagataa taacctctta gcattttttt cgtaaacaga ctatcttgct
    10321 ggatcatcca cgaagaaatc aaattccccg agttcatttt tattccttca agtttgcgtt
    10381 gctggataaa aaattgaacc atcgtttcat cataaatatc agtcaagtta tctaaacttt
    10441 caataatcaa ctgagtattt ttttgatatg tctctctgat ttcttggata tcttcatcac
    10501 taataaatgt ctgacgatca gcatatcttc tttgccaaaa cattttcgga tggagaacga
    10561 ttcgcattaa cctacggaca acaatgaacc aaaagccgat aaaagacaaa atctggcttg
    10621 tgatagaatg ttcgaatcga gaaagaaaac gggagtaaaa acgataacca ttagaagaaa
    10681 gcttgcccga acgataactt tcatccaatc catcccgttc gatcgacaaa atcagtgctt
    10741 gtatttcttg tacttcttgc ttttcatcat cggttaaatc ttccaagtac agatcacgta
    10801 tccgttcttg ataattttct atcactgctt cagtggctaa aaattctttt gtattacgat
    10861 caatttcctt tatttctttt cgcaaaactt caacgacctc ttctaaaatc atcaaagcat
    10921 tcaaatccgt gcttttcgct tcctctgttt ccgtcagcat aggcaagaca atgatcccta
    10981 tgaccaaagt cactaagatg acacccgccg tcagaaacaa aagcaaagac cgctggtaaa
    11041 acatcatatt attgattgaa aatggcaaaa taaaaatcgt agccaaactg actgttccct
    11101 ttacaccgcc aaatgttaag atcaagatct cgttcaactg ttttttgaac tttttactgc
    11161 catctttgaa tacataaaaa agactgatag aaatgaatct gatcacaaaa agcatgacag
    11221 agattaatac gataatcgct aataaaagac cgtttggata taaaccgtct ccccatactg
    11281 gcgagaacac ttgcgtcaat tcgattccta aaaatataaa tactaatgca ttcagtgtaa
    11341 aagcaatagt tgtccatgta ctgtgggaca agctcgacaa ttctgcatca aacacagaga
    11401 ttttcctaaa accagaagct tggagaatac ctgctgccac tgcagcaatg attccagata
    11461 catcaaatgc ctctgccaat acataggcta gaaaaggaag caaaagttcg atcagcagat
    11521 accctgtgac atcttgtgct gatgcttttt cgatcaaatg aattattttt tgtttgaacc
    11581 agacaagcaa aaagccgatc aaagctcccc caatactaga aactactaga ctcatccccg
    11641 cgtttactgc tgaaaaagat ccggtgatca gcgcaccaag agcaaactgg aaggcagtaa
    11701 caccagatgc atcattcaaa agaccttctc cttccaaaat atgcatcgcc tttttaggga
    11761 tattcactcg tccagaaaga gaactgactg ctaccgcatc tgtcggacct aacgctgctc
    11821 caaaagcaaa gcaggcagca agtgggacac ttggcaacag gaatgataac gtcgcaccta
    11881 ccccgacaag ggtcaaaatg actcctccaa atgccaagaa caaaattgtc ccgaaatttt
    11941 tgagaatcga agaaatatct gctttttctc cttctctaaa taacaatggt gcaataatca
    12001 taactaagaa caattcaggt tcaaaatcga ttgattgtcc ccactctgtc agacctaata
    12061 ccactccaag gaaaatttgg atcagtggag ctggaatcat tggcaaaatg cggctagcta
    12121 tgttcgataa ggtgatcgtt actgcaaata caataactaa ctcagcaaat tccatcgtcc
    12181 atccccttta ttttttattt tgtgtaagca tttttaaaaa ttgttctttt ttctttaaaa
    12241 ttttggtttc gactacttca acctgtgttt ggttcccctc taattcacac tgtacacgtt
    12301 ttgactccaa acgaagcaga tcatgtttca gacgttccaa ctgacgatac atcaatatat
    12361 ttcttttacg caacttacaa agtatttgac ggcggtcatc catcgaaagg gcgccatagc
    12421 gatcatgcaa cagttttttt tgttgttcaa aactctctct cattttccag atctcctata
    12481 cacaaacttc catcattata gcaaagttct ttttggatga aaaatatttc attgtttcat
    12541 cgtttattgc tagttagaaa tttccataaa tttttcaacg cctcattcta ttccaaatag
    12601 tatcagatat caagtaaaac ttaagaaaga taaaagtttc tgacaaaagg atgtttactt
    12661 tcccattcct gcctttcttg ttcttttatc acaacaaaaa aatggggctg cgacaaaaat
    12721 cttgtcacag tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat
    12781 aagacacatc tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc
    12841 ccgtatacat cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt
    12901 tcgctgattt ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat
    12961 gggaagtaca caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct
    13021 tcgtatttca atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt
    13081 gtcgcaactg cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt
    13141 tcaacaaatt cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga
    13201 tcgccgatat gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc
    13261 gctgtcattt ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac
    13321 ctcagtttag cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt
    13381 atctgcccac tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc
    13441 aatttttctt tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct
    13501 acgacggctt tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca
    13561 gtttcttcta gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa
    13621 cgatcatagg attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat
    13681 tcttctactt gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct
    13741 ccagtttctc gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta
    13801 gaacacttcc acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg
    13861 atcaataatg cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa
    13921 cgtaaaagtg cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca
    13981 ataaatgcat ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca
    14041 tttatacgtt ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag
    14101 gtttcttcct ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt
    14161 aaggcatctt catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct
    14221 agatcttgca gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca
    14281 ttgatcgtaa aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga
    14341 tctggtctac ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt
    14401 tcatcatttt ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga
    14461 aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc
    14521 tggttcagca agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc
    14581 tctaattttt tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc
    14641 atttttccaa cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga
    14701 ctgtttgaca agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg
    14761 tccttacgct caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa
    14821 acttcagtta agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca
    14881 ccaagttctt ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa
    14941 gtgtggttga cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct
    15001 aatggagaga aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata
    15061 ctgtataaag aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt
    15121 aacgcaaatc ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag
    15181 gaatttctaa atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca
    15241 aacgaaaaac agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg
    15301 gtcttgttta cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct
    15361 tgatcttaag agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc
    15421 cattgatttt actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag
    15481 gtacactttc tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt
    15541 tacagcatga tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg
    15601 ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag
    15661 aacaaaatta cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac
    15721 ttgcttcatt aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg
    15781 ttttcagtcg tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg
    15841 tcgtctccaa caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg
    15901 tgactttcct ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg
    15961 tcgttagctc tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag
    16021 ctttcctttc aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc
    16081 cacagacaag attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag
    16141 attccacatc cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta
    16201 tgttcactta cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca
    16261 tcagataatt tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca
    16321 taaagcattt ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca
    16381 gtatcttctt cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat
    16441 agatattctc ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc
    16501 caattcaaac ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa
    16561 aacaatttca tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca
    16621 aaattcgccc cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa
    16681 ttcaagatcc aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa
    16741 aaattcttct tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta
    16801 ataatgacag tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt
    16861 gcgctctttt cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg
    16921 gttcatgtga tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt
    16981 ctaattcatt ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca
    17041 tctgattgat cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta
    17101 aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt
    17161 ctgctcgctg tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga
    17221 cttggtaagg attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt
    17281 cttcttcttg aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt
    17341 ctcgaagttc ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa
    17401 aagctagctg gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta
    17461 cgatagctgc ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg
    17521 agattcctga aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct
    17581 ctttgaaacg atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat
    17641 caggaagcaa cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa
    17701 cttggtacaa atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca
    17761 ctttttccag gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta
    17821 acgggatgtt cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt
    17881 ttgcttcgat caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg
    17941 ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg
    18001 ataaagcttg caacattttt tcgctatttg tattcatttc aattccctct tatctcactg
    18061 acgatttgtt gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat
    18121 ccgtctatta tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa
    18181 agaacttttt ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca
    18241 agcaagtcca gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga
    18301 ttccacacga aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg
    18361 ccattttcgg tcagataaaa agcttgttca aacagtttac cataatttga caggttttct
    18421 gcctctaatt gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt
    18481 aattgctttt tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc
    18541 attccgcaac agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg
    18601 attgtttgat cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt
    18661 ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca
    18721 gggatcatcc atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc
    18781 atgatccctt catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg
    18841 acgaaatcaa gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata
    18901 taccagtcat tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca
    18961 taatagcgct gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga
    19021 ctgactagcg tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt
    19081 tccatacgca tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa
    19141 agataagagc aaagctcacc tcataacttg caaataaata aaaattaaat aaaatacatg
    19201 tgttttctat gtatgacata cggattcgga atctctgtct cttatttatt ccttttctgc
    19261 taggaaataa aaagaaaagc acttgtagcc ctagagccac aagcgctttt tcaactgtct
    19321 tatttaacag cgtcttttaa tgctttacct ggtttgaatg caggtacttt gctagctgga
    19381 atttcgattt cttcaccagt ttgtgggtta cgacctttgc gagctgcgcg ttcgcgaact
    19441 tcaaagttac caaaaccgat taattgaact ttttcgccgt tagctaaagc atcttgaata
    19501 gttgaaaata cagcatctac tgctgcagtt gcatcttttt tagttaaatc agtagctgaa
    19561 gcaacttttt cgattaattc tgctttgttt gccatggata gattcacctc ctccacaaga
    19621 gatatgatag aaatatcatt tagttaaaac tttctgagtg gtccagaagt ttaaaaagaa
    19681 atattgatta acttcaatat tctgttatca cgatatcata tgaaccgctt aatagcaagg
    19741 tttttaccaa ttttcacatg ctttttttag tcatagaaac gtaacaaacg aaactgtctt
    19801 tttcttttga aaaaaaaacg tcgatatggt aaaatgccta ttttctccta cgagggataa
    19861 ttcggatgga cgtcccttca aaagtaaatg cttttctgat ttgattttct aagaaacgtt
    19921 cataagaaaa atgcatcaat tcctcttcat tgacaaaaac gacaaatgta ggtggtttga
    19981 cagcgacttg tgtagcataa aaaatcttca ggcgttttcc tttatctgtt ggtgttggat
    20041 tgattgctac agcatccata atgatatcat tcaacacagc cgaagagata cgaagattct
    20101 ggttcatact tacttgttcg atcatttcag gcagacgttc caaccgttgt ttagtcactg
    20161 cagaaacaaa aacgattgga gcgtaatcaa gataccggaa ttcatcacga atctcttgtt
    20221 cgaaatcacg cattgtattg gtttctttct ttacaagatc ccatttattc acgacgataa
    20281 taattccacg accagcttca tgggcgtaac ccgctactcg cttgtcttgt tcccgaatcc
    20341 cttcttcagc attcagtacc atcaaaacga tatcagaacg gtcaatggca cgcattgcac
    20401 gcatcacgct gtatttttca gtattttcgt atacttttcc gcgtttacgc ataccagctg
    20461 tatctatcat taagaatttc tgaccacttt cagaaacgaa gtgggtgtcg attgcgtctc
    20521 gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc tcctaaaatc gcattgatca
    20581 aagaggattt accaacattt gggcgaccaa tcaaactaaa cttaatcgtg tcgtcgtcct
    20641 cttcttcagt ctcttcagag aaatacttca ctgcttcatc taaaatatca ccaatcccta
    20701 gtccatggct tcctgaaaca ggataaggat cgcctagccc taaggaataa aattcataga
    20761 tatcgtttct catttctgga ttatctactt tattgactgc taaaatcact ggtttattac
    20821 ttctgtataa aattttcgca accatctcat ctgcgtctgt cacgccttcg cgtccgctga
    20881 ctacacaaat gattacatct gcttcttcaa tcgcgatttc tgcttgatgc ttgatctgat
    20941 ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc gatcacgctg aattctcgac
    21001 ctaaccattc accttttgca taaatacgat cacgagttac tccaggagta tcttcgacaa
    21061 tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga cttaccgacg ttcgggcggc
    21121 caacgattgc aattgttgga tttgccatat tatttcctcc tcacaagttt ccaatctcac
    21181 ttagtttaac gaatgcattg ccaactttca agcatagatt gtcaattaaa cgaagtattt
    21241 ttattcctta ttgaaatact aatcgctttt caaaagaaaa actgagacaa aagcagctgc
    21301 tttcatctca gtcacagata caaaatcaat tatttttcag aatcatcggt tgctttttct
    21361 tctacttctt ctttcaacgc atcacctaaa atgtctccca tagtaaatcc agtattttct
    21421 tctggtaatt cataagactc aacatttttt ggttcttctt ctgaagctgg tttttcttct
    21481 aaagctttga tactcaatgc aatacgatgt tcttctggat ggatctctaa tactttcact
    21541 ttcacttcgt caccttcatg aagcacttca tgtggtgtcg caatatgttt atgtgaaatt
    21601 tgagagatat ggactagccc ttctactcca gggaacactt caacaaaggc accgaagctt
    21661 gtcaaacgtt taactgttcc gtctaatact gtgccaactg gagcttttgt atcgatttct
    21721 gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa cacgttcttg ttcaggattg
    21781 atagacaaca ctttgacatt tacatcatca ttgacagcca atacatcact tggttttgct
    21841 acatgactat gtgaaatctc tgagacatgg actagaccgt caattccgcc taggtcgaca
    21901 aatgcgccaa aatcagtaag acgtgcaaca cgtccactga gaacatctcc ctcatggata
    21961 gttgaaagaa tttctttttt cttttcttct ctttcgtttt ccactaccgc tttatgcgat
    22021 aagatcaaac gattttctga tggttcgatt tcgatgattt taaagctcaa tgttttgcct
    22081 ttatagtctg aaaagtctgc aacaaaatga tcttccacca ttgatgcagg gacaaaacca
    22141 cgtacaccga cgtcaacgac taaaccgcct ttgacaacat ttgtaacagg tgcttcgatg
    22201 accttgccct cttggaaatc tctttcgata tcttcccaaa cttttttagc atctaaacgg
    22261 cgttttgaaa gtagatagct gccgttttct ttatctttgc cgatcgtact gataacgact
    22321 aaatctaata catcaccgac ttttactact tcgctgatat cttctaccgg caaagttgat
    22381 aattcttttg ccggaacgac tccttcaact ccagcccctt cgattccgac aatcacttgt
    22441 ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc caacttttac ttcttgtacg
    22501 cttttcattg cgtcttccat tgactggttg ttctcttgat tttgatcaaa ttctgtcata
    22561 acacttgtcc tcctaaccaa cattctgccg taagtacggt actgttttta gtttaattga
    22621 ttattcaaaa aaaataaagc gatttcgttc gttttccttg aaaaaacaat tgtttttcat
    22681 tttttttctt aatttaaaaa agaagataac cttttttcaa aacaacagct tttattgctg
    22741 caacgacttc tggaatcgat ttgccagtcg tatcgatacg aacagcatcc tctgcttgct
    22801 tcagcggaga gacgtccctt gttgaatcat aatggtctct ttcctgtatc tctttcgtca
    22861 gtgtttcaag atcagtcgga attccttttt cttggttctc tttgaaacgt ctttctgcac
    22921 gctcacttac gcttgctact aaaaagattt tcacttctgc attcggtaat acagctgtcc
    22981 cgatgtctct tccgtccatg acaacgccac cgcgttcccc tatttttcgt tggacagcaa
    23041 ctaattcttc tctgactttt ccatgtgcag agacttcaga aacagctttt gtgacatctg
    23101 gttgacggat ttctaaagtc acatcttttg atgctatgaa tacatgctgt ccatcttctt
    23161 gttgcgaaaa agagatagga tactttttga tcagcgaaac tagcccttct tcatctgaaa
    23221 aagatacatt gttcgtaata gctaaataag ttactgcgcg atacatggca ccggtatctg
    23281 tataaatata attcaattct tttgctaata tcttggcaac ggtacttttc ccagatgaag
    23341 caggcccgtc gattgcaata ctgatttgtg tcatgatata actccttttt ctcagaaaaa
    23401 aagccgcaaa gcgcggctat tattatttaa ttcttaattc ttgaccaggg taaagcataa
    23461 agttgtttgg atctaagcca ttcaattcaa acaattgatc agtggtgatc cctgcacgct
    23521 cagccacttg ttttggacct tcgcctgctt gaacagtcgt tgttgaaccg gctgcagctg
    23581 atgaagaagg tgttgtttct gctgcattgc tgcttgaatc ccctgcttga taagttgcct
    23641 cacttgagga agtcggtgtt gtctctgctg caatactgct ttctgacgtt acacctgtat
    23701 cttgagacac agtagattct tccgcgattg tactagattc tactgttgaa gtctcagttg
    23761 actcctcttt tgttgtattt gtagaacttt ctactactga tgtcgaagtt tcagaagaag
    23821 ttgctgctgt tgcattgttg cttgatccat ttctgatcca gaaataagca cctgctggaa
    23881 tagcgataca taagaccagt aaaatcaaaa atagcgaaag aaataatgta tttcctttct
    23941 tttgttgtct ctgagaactt cttgatgaaa cttcatcatc tgtatcgtag attggttgtt
    24001 cccatggttc ttgtgtttcg ttattttctg tagattgatg tctatctttt ctgctcactg
    24061 ttttacctcc taaactctat cgtgtgtatt ataccatagg aaaaagtgtc ttgtcctaaa
    24121 aaatcaatgt tccatgtttt ttttaaataa atttagtaaa atcgtttccc aatgaagttg
    24181 ttcagtctta gaaaaaagct tcctctttgg cagaagttct gcttttgctc catcaatatc
    24241 acaacaattc tgcggctttt cggagagttt ttcgccaaaa taagctaaga tgaattttct
    24301 gcggcagttt tcttcattaa tatagcgaag catctgctgc aggcggaatt ctttttcttt
    24361 ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt tcagactgct ggatcagctc
    24421 catccatttt ttttgcagct catcaaaggg agcttgcttt gtggcatatt ccaaataaag
    24481 ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag aaataatgga tacgttcatc
    24541 cccactttga taaaaaagaa ttgctgcact ttcttcctga tctctgcctg ctcgaccgat
    24601 ttcttgtaca tagttttcta gactgtctgg caaatcataa tggaccacat aacggatatc
    24661 agatttatcg atccccatac caaatgcatt cgtagcaatg agaaactgca gctgattttt
    24721 tacaaactgc tgttgaagtt gtcttctttg agctgcatca agaccaccgt gatagtatcc
    24781 aactgtaaaa cgttccctga ataaatgata caatcgttcg acttcttttt ttgttgcaca
    24841 ataaatgatt gccgctcctt gtgctctttc catgaagtgt tcaagttctt gttctttttg
    24901 ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg actgattgtc tgatctcttt
    24961 tggtggtcga tcaaatagta catgacgtat atctcttgct actaaatctg ttgctgtcgc
    25021 cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg atttttccta actgttgata
    25081 ttctggacgg aaatcaactc cccattgaga cacgcaatga gcctcatcta ctacatataa
    25141 cgcaatattt tgttttttca gatgctctaa aagagaagga ttagtcaaca tttcgggact
    25201 caaaaacaaa aatttatatt gcgatagatg acggaggacg tatcttcttt cagattgcga
    25261 aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt ttttgtaatt gcgtcacttg
    25321 atcttccatt agagaaagca gcggtgacac gatgatgacc agaccttcca ttaaataccc
    25381 cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc aaaatagcga gagtatcttc
    25441 tccatcaagc aacgcctgaa taatttcttc ttggccggga cgaaatgaat caaatccaaa
    25501 atattttttt aattcattta ttaacattct gtatcgcttc ctttctcaat agatagattt
    25561 gatacaaacg aaattctcca taatctaatt gtccatctac attccattcg ctaaagcgcc
    25621 actctcgcac ggaatcctta tttggttcca aacgttcgat tgtttctgca gagagaatcc
    25681 actcgtatgg aaaatcatca aaaaacaagg cccactcaat aagatgatct gttactgttc
    25741 ctctttttaa atgacggatt gccatgatct ggtcaattgt ctgaccattc ataaacatct
    25801 gcttggtgat catcatgctt tgattgaaat tttgctgtaa taatgaatcg atcaaagcgt
    25861 gccaaagcga atctggccgt ttttcgattt gtgctaacaa aaggtggata catctgcttt
    25921 gttgcagata aacctctgac tcatcattgt tttttgccag ctgataagga agcagccctg
    25981 ttcgctcatt tccagataat tggttagcta aaaaatctgc tgcttcttct ggtatctccg
    26041 aaaacagctg caccaaactc tcatacgcag tatcaattaa aatttcacgt ggcaattggc
    26101 tttcactaag ccattttttt agttggaaag tataaaaagg acttgtctct gctggaagat
    26161 agtcttgaat acccgttgcc agattagaaa ttacttgaac agcaaattta atgagccgcc
    26221 aactagtttc ccctgttctg ccatagcggt caaaacgcaa accagtatgt tctatttgat
    26281 gttcggacaa catatcagcc cctgcaggcg ttagctttgc ttcattttca tttatttcga
    26341 tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa cttatcctgc ttcaattctg
    26401 gaaaactgtt atggataaac aaaagttcgt gggtaaatcc ataaataagt actgaagtcg
    26461 ttcgttttcc cacaaggagt tggtaaagag aactaatccg cagcttgtgt cctgatgaaa
    26521 atagcgataa aataaaaaca atcatattat tctccttaag ttaggatggt tagaaatgtc
    26581 actactatgt aaacttgtac ctgaacgctg tattgcatgc ggtctttgtc aaatcgaagc
    26641 gccagatatt ttcgattatg atgaagaagg aatcgtttta tttgcccaag aaccagaggc
    26701 tcatcaacag ttcgtgatgc cggaagaaga aacatatgta aaaaaagcgt atcaaaaatg
    26761 tccagttcga gcgattttat tagaaaataa aatataaata taaacgaaac tttaaaaacg
    26821 tacactaagt tctctaaact tgaaaataag ccgggatctg tttctcttgt tccgtgaaat
    26881 cccgacttat tttgaaagat tcatttgcta tctatagtta attgactagt tttttcacgg
    26941 accctatagg acccatttat attatttcaa ctttttcagc cagttttcca atgtattgac
    27001 gatcacagtt tgctggtctg cgttagaaat ttctgcctct ccatctcctt tttgctcacc
    27061 atagctgcca aaacctccat gatttccgcc attgattgat acatactctg ttctttccgg
    27121 taaatactgc ttagcttctt gataagcttc ctgattcaat actttatctt ttgtcgccgt
    27181 gatcgataaa acaggaacag ctgattctgt caatgttcct tttttgtcgg gataacttgc
    27241 catgaagaat acccctttta aatgtttgtc tgaatgattc ttattgacat aacgacttgc
    27301 cattacgcca cctaatgaat ggccaccgat cacataatcg gaatttggaa aatcttcttt
    27361 gaccttatcc gctttgtttc cgcccatcac tgctagatca agtggcattt tgacaatagc
    27421 gactgaaaag ccgttttttg ctaaatcttg cgcccaaata ctataacttt ctggctctac
    27481 gagagcacca ggataaaaaa taagtaatgg agactttcca tgattctttt tctctccttc
    27541 aaaaaataag taatcttctt tgttttttcc ttcttttacc aacacttgcg cttctgacga
    27601 tggttcgtac gtattttgct gtatatagta ataaccggct ccaccgagta atcctaaaat
    27661 gatgactaag ccaattagta ttcgtctaaa tattttcact ttctcccacc ttttctatct
    27721 gagtgacccc tttttcttaa tcaagtatga ttatacctta aaaaaataag agagaaatgc
    27781 aaactttcca gcatcaaata aaaaaagacg gaatatccat ttgaatatcc gccctttttg
    27841 tatgtttatt tttatttagc acataaacga tacacagctt ctgcataaat ttccattgct
    27901 ttatacatac tacttaattc ccatcgttca ttcgcctggt gcataaagtc aggagtatca
    27961 gggaacatcg caccgaatgc cacacactgg ttcatcgtac gagcaaaagt agcgccgcct
    28021 gatacaaacg gttcagtcat gtctccagta atatcgcgat atgtccccaa tagatttttc
    28081 actaactcac tgtctagtgg cacgtataat gaatcaagga agtcaaattc ttcataagtc
    28141 aacccatatt tttctgctgt tttagttaat ttagctacta aatcatcttt cttgaatgta
    28201 acagggattc tcatatctac accgattttt gtttgttctg gtgtgatttc caaacttgca
    28261 aagttcatcg ttagctcgcc ggattgttca tcttctgttt tgcccacgac agcttcacca
    28321 gttgcatttt cttgaactaa ttttcctaag aaattgattg ggccaaaatc aaatacttct
    28381 gaaagagcaa ttgccagacg agaaatagca ttcactcctt gtgctgcatc tttcgcgtgg
    28441 atacttttac caagaacgac gatgccttca ccttgttctt cgaaatcgaa ttcgtgtttt
    28501 ttcaatgctt cttttacttc agaaagcttt tctccagagt aaggagctgc atcaggaaca
    28561 acattcaacg ctcctccagc ttttacagaa aactcgttcg ttcctggtcc tgtcaagtaa
    28621 gcttgcaata agcctttttc tgcataaatt aaaggaaatt cagcatctgg cgcaaatcct
    28681 tgcgtaattc cttcttcttt ttcgttatat ttttctaaac aacgccataa attttcttca
    28741 tctgtaccaa agataaaacg aatacgtgtc ttgaattcca cgccagcatc aatcaaagct
    28801 ttgacggcat acattgccgc aatagaagga cctttgtcat cttgggatcc gcgaccaact
    28861 aaccagccgt cttttatagt tggatcgaat ggttttgttt cccaattatt ttcgtcccct
    28921 gctggaacaa cgtccatatg acacaagata ccgaataatt catctccaga gccaatctct
    28981 gaataaccgt aatagccttc tggatcttta aatgttctaa atcctagctt ttcactgatt
    29041 tccaatactt tatccaaagc ttcaatcact tttttaccga agggatgtcc ttgtcctgaa
    29101 tctgcttcat ctagtacgga aggaattcgg atcaattcgc tcaatgattc taatgcttgt
    29161 tcatgatgtt gttctgtcac aaattttttc atgatattat cctcctaata tgttaaaaag
    29221 ggtcatgtca aattcctccg acacaacctc ttcttctcaa ataatgacgt tttaagcctg
    29281 ctccatcagc aagcagaatg ttcttaacaa atgatgaaag aaaaaacact cactgaatgg
    29341 agctattgac ttatccacgt gcttatcgat agccgtttat cggtttcttc attcttatga
    29401 tagaccttgt acctgtattt gtctaggttt agaacattgc tgctacacct aggatcacac
    29461 agatggctac aaataatatc gcaattaatt taccggtaaa tttccaccac actgaaacat
    29521 cgaaacgagc aattgctaca gcacccatta cgatagcaga tgttggtgta attaagttta
    29581 ctaaaccaga tgcagattga taagcagtga ttactaaatc cttaccgacg ccggcaaatt
    29641 cacccatagg tcccatgatt cccattgttg cagcagctaa gccggaagtt gaaggaatca
    29701 agaatgacat agggatatag aaaataaatg taataatgat gaatacactt gaagataatg
    29761 aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt gattaatcca ttgttcataa
    29821 ccacttggat accacgagca acagctacta cgattgctac accgatcaaa tcagaagcac
    29881 cagctaaaaa ttcagagaca aattcagact ctttcatgcc ataagtgatt gctactacga
    29941 tggacattac taagaataac atagtaattt ctgggaagta ccagtcacct aatggaagca
    30001 tgcttttacc tagaaccgta cctagtaccg gaaggtttgt taaccagttt gtcaatgatt
    30061 caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat caaactgatg atcatgatac
    30121 caaaagtaat aaagaacaat acattaacat gtttttgttt tttcgtgatt acttcttgac
    30181 gaccgattgc ttcaatatcg aaatgcttca tatcttcttt acgttggttg tatactaatg
    30241 attttgttgg atctttttcg atctttgatg cataacgata cacatataca atagaaatag
    30301 ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc ttcacccatc gaaatcccaa
    30361 cagcttgtga agctacgcct gttgcaaaag gatttaccgt tgaagctaga cagcctactt
    30421 gagaccctac taatacgatt gctacagcta ctactgtatc aaagcctaca gccatcatga
    30481 ctgggatcag taaagcatag aatggaatcg tttcttctgc cattccgtaa gtagagccgc
    30541 ctaatgcaaa taacagcatc aatactggaa taagcatttt ttcttttcct ttgaatttct
    30601 tcacaactga agcaatcccg gcatctagtg cccctgtttt gttgataata cctaaaaatc
    30661 caccaacaac caaaatgaag aaagagattg ggattgcagc aggtgtttct ttagttagct
    30721 cccccgctgg tacacctagc atcccattga ttggcgccat gaaaatatcc cacagccctt
    30781 gaggattttg ttgtactcgt tcatatgatc cagcgacgat gttgcctgca tcgtctacac
    30841 tatagtgccc agctggtata aaccaagtaa gtacagcgat tactgcaata ataataaata
    30901 agaccgtata cgctgaaggc atttgaaatt ttttcttttt tgtttgttcc atcttcttct
    30961 tcctttcttt tccgttagag acaacaattt tggctctaat gttattttat caaaaagagt
    31021 atcagaacaa aaataataac acgcattttg taaaactttt gttcaaaaag ttcgtttttc
    31081 cctattccct cctctttttt caggttttta aagctttctt ctttttcatt gtagcgtttc
    31141 ctttttttta taaacaaaca ttttcgtctt ttaaaaggag aagaatttca catttgcaaa
    31201 gaattgattg atttttatgc ataaatcaaa aaaatactca aaacaaacaa ataaaacgtt
    31261 tacattattg catgtcttgt atttttttcc aaaaaaaaaa gaccaagaaa tttcttgatc
    31321 tttttgatat ttatttgact gctgtatgac gttgttcgaa ctgatgttgt tttcgactta
    31381 accatggaag aagttgcgca tgcagcacta aaaagacagc actcacgatg aatcctttga
    31441 tcaagttgaa aggaacgatc ccaatcagta catagtttgc taaagacatg cccagcatct
    31501 gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt aataacgaaa taatttgcga
    31561 tactcataaa aatagttaga gcgatggttc ctgtaatgac acctaaaaat ttgtttcttc
    31621 ctttatgctg tttctttaca ctaaagaaat aataaatcgg gaatgtaaag atcgttgttg
    31681 ataagaaact agcagtgtct cctaccaaat tatccggaga aaaacctgta gtcagcagat
    31741 gtaatactga acgcaagaac gctgttgcaa ctccggccgc tggaccgaat aagaacatgc
    31801 tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa actaaaagcc ggcatgatcg
    31861 gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa catcgctact gcaaccattt
    31921 tctgtacacg ggtgttcttc atttgaaatc ctcctgttag gactcctctt cagcgaactt
    31981 tttgacgttt ctctccaaat gaaaaaactc tattttccag ggaaaataga gttagagtta
    32041 tttgatcaag cgtcgtcaaa taagctctat cttctttatc cagactatac tgtcggtatc
    32101 ggaatttcac cgattcagct atttttttgc aaaataggtc gtggactata accaccggtc
    32161 gggaatttca ccctgcccct gaagacgaac cttatataat ttttgtttac atattcatta
    32221 tacatatcta ttcaaaaaac gcaacatcta acacttataa aaagtaagtg ttcattttga
    32281 gtggttcagc aaactgctga cttctttttt gttcagttca cggtaatccc caggacgtag
    32341 gccttgtaaa gtcagatctc cgtatttttc acgttttagt ttttgcacag gaagaccaac
    32401 tgcttgaagc atatttttca cttgatgatt acgtccttcg tggatcgtca attccacgat
    32461 actgtggttt gtcttaggat ccactgataa aatttggaat ttagctggag atgtttttcg
    32521 tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc gattttgtag cgatcccttt
    32581 tacctttgca acgtaaactt tatccacttc atgttttgga tgtgtcaggc gctgagcaaa
    32641 atctccatca tttgtcagta gcaagatccc cgaagtatcg taatccagtc gacctacagg
    32701 atagatacgt tcttttacca tcggcaaaag atccgtaaca acttttcttc ctttgtcatc
    32761 tgcaacagca gaaataactc ctttaggttt gtacaataaa tagtaaccgt attcttcttg
    32821 ataaattggg acttcatcca cttccacagt gtcatgtttc ccaactttta tccctaattc
    32881 ccttacaact tgtccgttta cttttacacg tcctgcaagg atgaattctt ctgcttttcg
    32941 acgagaagtg atgccagcgt gggcaattac tttctgcaat ctctccatta caattattct
    33001 cctttattca ttccatcaaa gaatagatct ggtgacacgt cttcttcaag tgaaatttcc
    33061 atttcttgtg tatcaggcag ttcttctaat gatttcaaac caaaataatc taagaagtag
    33121 ccagttgttc catataatat cggccgacct ggtccttcta ctctaccttt ttcttcaatc
    33181 agtttatggg cagctagacg ctgtaccgct cctgaaacct gaactcctcg aatctgctcg
    33241 atttcagctc ttgtgattgg ctgtttataa gcgatgattg ctaaggtttc gacggcggct
    33301 tgagataatg tgtttgatgt cccttgcgca tatcgcttaa gtaagggagc cagctctttt
    33361 ttagtcgtta atacataatg gctttccgtt tcaaaaatat gcaaagctga gtgcgagttt
    33421 tgagtgtaag attcttttaa ttctttcact aaacttgtga cttctgcttc gctgagattt
    33481 aacagtccgc ttaattcttc attactgatc ccttcctcgc caacaataaa aagaagggct
    33541 tcaagttcac ttaatttcgt catgttcggt atctcctact gctggataaa gcataattga
    33601 ttcatagttt tctttttgtt gcgcaatgat ctgccctttt ttcatcaatt ctagcaaagc
    33661 cataaaagta gtgacaactt cacttttaga aggtgactca aaaaaagaat cgaaagtaca
    33721 gccatgcgca tttcttttta tacgctcttc gataaatacc attcgttctt cgatcgttgt
    33781 gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat ttcttacgtt ctaagatttt
    33841 ctgcatggca cggatcaaat cgattttttt gacttgtcct cgcttcaacg gcttttcagc
    33901 atccgctaac ccatcgatgt tcataggttc ttttgtgtaa taaagacttc gttcctctgc
    33961 tttttctgtc agtctttcag ctgcatattt atatttacgg tattctaaaa gctgagtgac
    34021 caattcttct cgaggatctt cttcccataa ttcttcatcg tctgtgacga attcttggac
    34081 aggaagcaat gtcttgcttt tgattgccat caatgtagca gccatcacta agtattctcc
    34141 tgcaacagct agctcgagtg ttttcattgt atggatataa ttcatatatt gctccgtgat
    34201 cgtagcgatc ggtatatcat atatatcgat ttccatttgc tgaatcagat gaagcagcag
    34261 atcgagagga ccctcgaata catctaattt aatattgatc tccgtcaact cattcactct
    34321 ttcttctttt tctttgctag ccattttcca atgtaaggct ctgtgatttc agttgcacgg
    34381 atggagacat ccgggtactt ttcctgtaat tcatctaaca tttgaaagcc catattctca
    34441 ccgcgataag acggattcaa agaaatgtca tggatgatca gctgatcgtt cgttgcccag
    34501 taaataccaa taatcccttg aatgttggtt gaaccttctg gcgtaaagaa aaaaagttca
    34561 taatcgtctt cagattcgta cgtatcaatc tccttcaaca aagaagtttt acctgtcagt
    34621 ttcttatgga aactcaatag tcccatagca attttacggt tttctttccg gtaatgtgtt
    34681 aacatcttct caccttccta tgactaagcc cttggaaaat gctgcttata aacatccgtc
    34741 attcttttct tcgtgatatg tgtataaatc tgagtcgtag agatatccgc gtgacctaac
    34801 aattcctgta ctgtccgcaa atcagctcca ttttctagca gatgtgtggc aaagctatgc
    34861 cgcagcgtat gtggtgttac ttctttgtaa atcccagcct ctcgtactaa ctgcttcaga
    34921 tttttccaga ttccttgccg agaaagacct ttcccatgat tattgacaaa tacatgcatt
    34981 tcagatgcgt cctttacaag taatggacgt gcttcgtcta agtagcgttc cagccattgg
    35041 atcgcataat cacctaaagg aatgatccgc tctttatctc cttttccaac tgtctgtacc
    35101 agtccaagag aaagatgaag atctcccaat ttcaatccaa tcaattcact gacgcgcatc
    35161 ccagtagcat acatgacttc caagattgca cgatctctta tccctaacgt ttttgtcgta
    35221 tcaggtgctt caatcagctt ttctacttct gtcagagtca atgtacttgg taatttctgt
    35281 gctttcttcg gcgtatcgat atgttgcatc ggatcatgat cagtgatacg ctcttgccgt
    35341 aaaaactgat ggaatcttct gagacttgag atcatccgag taacagttgc tgtcgctttt
    35401 ttttcttcat gcaacttctc cagaaaacgg atgactaaaa agcgatcgac atcctgccat
    35461 gaagcaattt tctgctcctg tagaaaatgc aagtactgtt ccaaatcacg ttcatagctt
    35521 tgtcgtgtat tggcggacaa tccccgttca atattcaagt agtggagata atcgttgatt
    35581 tgctctttca taataatccc cctcatgcct ctctatcata tcagataccg cttcattctg
    35641 atagtctttt tccataatct gagcaaagaa aaagccgtct agacactcag acggactggt
    35701 ttcttttttc tttttcatag cattcctgac agattccatg gaacgttagc cgatgatctt
    35761 ttactaaaaa atgataacgt tcttctacta cttgctccac ttcaagtaaa agatcctctt
    35821 ctacttcctc gatcgtgccg cagttcaagc atagtaaatg atgatggaaa tgtttggcgc
    35881 cttcttttct caaatcataa cgggctaatc catcattaaa acttacttta tcgacaattt
    35941 tcaattctgt caagatttca agggttcgat aaacagtagc aagtccaatt tccggactct
    36001 ttttttcact aaaaagaaga tttcttctgc agataaatgc tctttctcat tttccaaaag
    36061 cacaagcaga gtagcttctc tttgcggtgt cagcttaaac cccgcttcat gtagctgctg
    36121 ttttgttttt ttcatcgccg aaatagcatt catgaccatt tactcaaccc caacaattta
    36181 taataattac aaataaaaaa agatataaca ctatgttaat taaaattatt ataagctagt
    36241 taagaaaaac atgcaaggga ttctcaatta gattttttga ctagttttgt cacaccgttt
    36301 tcttgtgtaa tcaatccttg tttcaaaaga ttacctaatg cgcgcttaaa tgctcctttg
    36361 ctgataccaa aagctttcat gatttcttct ggatccgatt tatctgtaaa gttgatttgc
    36421 tgatctgcag aacgttctaa aatggttaaa atcatttgtg catcatctga tatagcttca
    36481 tatgctcttg gcttcaatga aagattcagt acaccgtctg gtcggacacc aatcactcga
    36541 ccttttatct gctcgcctag acgaggttca ttatatcgtt cagaaggatg gataaaccca
    36601 agatagaaat cttccgttaa aacgtacgta cctgttaatt tcaagcggta tgctgttcct
    36661 atcacatctt tgtttttcat ttcttctgtt ccatatttgc ttaaagattt gaatattttt
    36721 tcatctgcta aactgcccca aatacggtct tttgcatcaa ctctaaggga aatcatcaag
    36781 cgatcccctt ttttaggcca tagctctgtc attgtcggca attcatcaag agatacgaca
    36841 aagtctttat ctggaagacc gatatcgaca aacgcaccta aatcttttcg tgtagcagta
    36901 acgattccaa aagcgtaatg accgatacgg cttttaggaa tttgtgtagt gatccgattt
    36961 tcatgttttt gattttggta accgaatcct tcaactgctt caccgatttt gtgttcacct
    37021 tctgatttgc ttaagccaaa ggtttgtccg tttttttgga caaagtagtt tttttcattt
    37081 tcatcaataa taatacctgt aaatatagtt gctaataaat cgttcatttt tttctccctc
    37141 tatagtgaaa ggttttctta atacatttct ttgctatcat aacataatgt tatctacttg
    37201 aatagctgag ccttggtttg gaaaggtgaa aaaaggagta tgacaacagt tgtgtcatac
    37261 tccccttttt cgaataaacg gtgttaaacg gcaggctcac agcctctttc atttgcctaa
    37321 aattaattgc tcccatttcc tccgaaccat ccagttttac agttgatggt tacgatgtaa
    37381 aatggcaaag cactttctcc aatgcctttc atcaatgcag attttgttga agtggcatat
    37441 agattataat agccttcccc attttcgatt ctggcttttt ctaatctgta acctccttca
    37501 acgatatatc cacgttggat acatgtagca atcactttgc tttggacacc tggtgcccac
    37561 gtccatgctg gattagatgc atcggcaata tcacttgctc tggcagattt gattggctga
    37621 ttcgctgagt ttgaagagga aggggtcgag gatgccgctt cttgctgtgc tgcttgtgcg
    37681 accgcagctt gttgagcggc ttgagctgca gctgctgctt gagcggcttg ttttgcttgt
    37741 tcttcagctg cttttttcgc ttcttctgct tttttctttt cctcttcttc ttttttctct
    37801 ttatcagtga gcatcttatc tacttgtttc aattgttcat tcaactgagc agatagatct
    37861 gtatttttca ctttttctac tgcttctttt gccgtcttgt attggtcgcg tgttgctgaa
    37921 tccactactt ttccgtcttt aaagactata gctgtttttt ctttagctgt ttgcaattca
    37981 gcatattgat ttttagcttc tgtttgtgct tgttgaatca attttccaaa atctgtgtta
    38041 tccgtttcga ttgcttggat ctctttatct gctttcaact tgatgtcatc tgctagatga
    38101 tcatcgacga tggcaggtgc tatgaaccac tcattcactt tttggatatc tcggatctta
    38161 gattggatat cttggtaagt tgtttctaac tctgaatatc ctttttcatt cttaagactg
    38221 gataaatcat tttttagttt tgataaatct tgatgaatca tatctgcacg aataaattga
    38281 tggtgatcat ctgtataaaa ggcagcaagt ttctcatgaa gttcttccat ttgttgctgt
    38341 ctttttgctt ctgctgctac ttcggcttct actttttgat gatggtcata ataagcccag
    38401 cttcctccac cgagtagaag tactgctgct aaaacgagat aaggtacttt ttttgatttt
    38461 ttcttttgcg ctttatctgg atctgttttt tctaagtttg attcttgatg tgttttttca
    38521 gtatctggca cgattgttat tgatttcgct tctgcttcaa acagcttagg ctggttctct
    38581 tctttttcta tttttgcttc aagatcagta ccctctttgc ttacagattg attttcagca
    38641 tcggattcac tatttatttc atcgggctct gttgactttt tgtctgtgtt ttcgagtatt
    38701 tcttttttct ctgcttccgg cagtttattc gttttttcct tttctaattg tttttcagta
    38761 aatacttcag ttttttctgt gatatccgtt tttacgtctt ttatttttac tgtttcttta
    38821 cctgtggcgt tttctctatc agagtctttt tcagtttctt gttccttttc tggctctact
    38881 ttgttttcct gtgcaatttt ttcactgatc attttgagca gctctttcgc gtgctcttct
    38941 tttgtatctt cacgatgtgc tttgatataa gcggatagta tcgaattttc ttcctcatta
    39001 tcgacgattt ctttcagttc agctactctt cttttttgtt ttgattcaag cgtttcttct
    39061 tgattctttc ctgattcttc cgaagtctca tgaacagttg ctttcttctg ttcatttttc
    39121 tctatattag ttacttgatc gtgagcaggt ggttcttcgt cttctggttc tgctttttcg
    39181 aataattcca ttacagattc tagaggtaaa tctttcaatt ccgaccattc gattggatcg
    39241 tttaattctg tatcaggaaa gactgcatct tcagttgatt tatcctgcat agctgcatct
    39301 gtatttgttt cttcaattga ttcttttatt tgctgatctt ctgcagttat ttgatgaccg
    39361 cagtctggac agatagtatt ctgctccatt tttttcgatc cgcaatttgg acattttttc
    39421 atcacttagc gatcggctcc tttactaggt ttacgctacc atttaatcta tcatattcta
    39481 tacgtcacgc caatagctta tttttaggat ttacttaaaa ataatgaatg aatttccttc
    39541 ctatagaaaa aaaagcttga gactgcgtag tctcaagct
//
LOCUS       DAZKSU010000018        38818 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000018 DAZKSU010000000
VERSION     DAZKSU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.018"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="ACG8Z3_000986"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="ACG8Z3_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0823322.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="ACG8Z3_000987"
     CDS             complement(2204..4390)
                     /locus_tag="ACG8Z3_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0823323.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="ACG8Z3_000988"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="ACG8Z3_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0823324.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="ACG8Z3_000989"
     CDS             complement(4612..5052)
                     /locus_tag="ACG8Z3_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0823325.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="ACG8Z3_000990"
     CDS             complement(5126..5665)
                     /locus_tag="ACG8Z3_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFQ0823326.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6280..7746)
                     /locus_tag="ACG8Z3_000991"
     CDS             complement(6280..7746)
                     /locus_tag="ACG8Z3_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0823327.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8056..10137)
                     /locus_tag="ACG8Z3_000992"
     CDS             complement(8056..10137)
                     /locus_tag="ACG8Z3_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0823328.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10279..10401)
                     /locus_tag="ACG8Z3_000993"
     CDS             complement(10279..10401)
                     /locus_tag="ACG8Z3_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823329.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            complement(10661..11020)
                     /locus_tag="ACG8Z3_000994"
     CDS             complement(10661..11020)
                     /locus_tag="ACG8Z3_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0823330.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11050..11391)
                     /locus_tag="ACG8Z3_000995"
     CDS             complement(11050..11391)
                     /locus_tag="ACG8Z3_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823331.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11388..12062)
                     /locus_tag="ACG8Z3_000996"
     CDS             complement(11388..12062)
                     /locus_tag="ACG8Z3_000996"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0823332.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13239..14537)
                     /gene="asnS"
                     /locus_tag="ACG8Z3_000997"
     CDS             complement(13239..14537)
                     /gene="asnS"
                     /locus_tag="ACG8Z3_000997"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0823333.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14577..15767)
                     /locus_tag="ACG8Z3_000998"
     CDS             complement(14577..15767)
                     /locus_tag="ACG8Z3_000998"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0823334.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFLKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15788..16315)
                     /locus_tag="ACG8Z3_000999"
     CDS             complement(15788..16315)
                     /locus_tag="ACG8Z3_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0823335.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16362..19151)
                     /locus_tag="ACG8Z3_001000"
     CDS             complement(16362..19151)
                     /locus_tag="ACG8Z3_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0823336.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEMELFIYVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMTTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19298..19498)
                     /locus_tag="ACG8Z3_001001"
     CDS             complement(19298..19498)
                     /locus_tag="ACG8Z3_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0823337.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19950..20270)
                     /locus_tag="ACG8Z3_001002"
     CDS             complement(19950..20270)
                     /locus_tag="ACG8Z3_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823338.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(20654..21772)
                     /locus_tag="ACG8Z3_001003"
     CDS             complement(20654..21772)
                     /locus_tag="ACG8Z3_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823339.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            22005..22127
                     /locus_tag="ACG8Z3_001004"
     CDS             22005..22127
                     /locus_tag="ACG8Z3_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823340.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            22243..23550
                     /locus_tag="ACG8Z3_001005"
     CDS             22243..23550
                     /locus_tag="ACG8Z3_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0823341.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            23669..24868
                     /locus_tag="ACG8Z3_001006"
     CDS             23669..24868
                     /locus_tag="ACG8Z3_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0823342.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(24895..26163)
                     /locus_tag="ACG8Z3_001007"
     CDS             complement(24895..26163)
                     /locus_tag="ACG8Z3_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0823343.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(26218..26284)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(26326..26973)
                     /locus_tag="ACG8Z3_001008"
     CDS             complement(26326..26973)
                     /locus_tag="ACG8Z3_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFQ0823344.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALATEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMIYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27381..27884)
                     /locus_tag="ACG8Z3_001009"
     CDS             complement(27381..27884)
                     /locus_tag="ACG8Z3_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0823345.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28007..28771)
                     /locus_tag="ACG8Z3_001010"
     CDS             complement(28007..28771)
                     /locus_tag="ACG8Z3_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0823346.1"
                     /translation="MKEIQSAKNPKIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            28879..29154
                     /locus_tag="ACG8Z3_001011"
     CDS             28879..29154
                     /locus_tag="ACG8Z3_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0823347.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29592..30542
                     /gene="yidC"
                     /locus_tag="ACG8Z3_001012"
     CDS             29592..30542
                     /gene="yidC"
                     /locus_tag="ACG8Z3_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0823348.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYHENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(30721..31695)
                     /gene="lacD"
                     /locus_tag="ACG8Z3_001013"
     CDS             complement(30721..31695)
                     /gene="lacD"
                     /locus_tag="ACG8Z3_001013"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0823349.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPECGLPAAALREKECGLLLAYEKTGYDTKMPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECDAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLKETATPVYVDR"
     gene            complement(31692..32150)
                     /locus_tag="ACG8Z3_001014"
     CDS             complement(31692..32150)
                     /locus_tag="ACG8Z3_001014"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0823350.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTEIATILTKEIGEDL"
     gene            complement(32147..33610)
                     /locus_tag="ACG8Z3_001015"
     CDS             complement(32147..33610)
                     /locus_tag="ACG8Z3_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0823351.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLVGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(33592..34542)
                     /gene="pfkB"
                     /locus_tag="ACG8Z3_001016"
     CDS             complement(33592..34542)
                     /gene="pfkB"
                     /locus_tag="ACG8Z3_001016"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0823352.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            34685..35433
                     /locus_tag="ACG8Z3_001017"
                     /pseudo
     CDS             34685..35433
                     /locus_tag="ACG8Z3_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            35699..37177
                     /locus_tag="ACG8Z3_001018"
     CDS             35699..37177
                     /locus_tag="ACG8Z3_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0823353.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
     gene            complement(37245..38336)
                     /locus_tag="ACG8Z3_001019"
     CDS             complement(37245..38336)
                     /locus_tag="ACG8Z3_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0823354.1"
                     /translation="MLKKNTSLQNKTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFLFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
     gene            complement(38374..>38818)
                     /locus_tag="ACG8Z3_001020"
     CDS             complement(38374..>38818)
                     /locus_tag="ACG8Z3_001020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0823355.1"
                     /translation="QLQNVIFLLGGQSNPYKYMSRADLYVMPSITEGFPNALVEAMST
                     ETAVLCADCKSGPREILSNRTLDTICENIEYAESGILVCPMTDSRNYSPQYEECDKNL
                     ANAIEELIENDNLRNKYAKAAKKRAELYSYDAFEHNLLQIMKTFN"
BASE COUNT        11293 a         8233 c         6717 g        12575 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6121 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttctgcgaa aaacaagccg
     6181 aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     6241 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
     6301 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
     6361 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
     6421 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
     6481 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
     6541 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
     6601 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
     6661 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
     6721 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
     6781 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
     6841 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
     6901 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag gaatattatc
     6961 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
     7021 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
     7081 aggggatatt ttttccgctc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
     7141 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
     7201 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
     7261 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
     7321 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
     7381 cgtatttacg atatttccat ttccttggac tgcccaaccg ccagcaatca ggattgcttg
     7441 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
     7501 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
     7561 gaaggctgac cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
     7621 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
     7681 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
     7741 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
     7801 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aatacacatc
     7861 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc atttacctta
     7921 ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta ttttataatc
     7981 ctatctattt tctttacatt tttcattaag aaaaggatat aaaaaaagag gacgttcatt
     8041 cgtcctcttc attgatcaac gtttattttt tacatgcaag cttcgcatgg cattcaaaac
     8101 agctaatact gtcaccccaa catctgcaaa tactgcatct cccattgatg caaaaccaaa
     8161 agcacctaag gcaagtacca aaatttttat cccaatagca aagacaatgt tctgtttgac
     8221 gattttcaat gttttctttg ctaaatggat tgcttctgca atcttcgatg gttgatcatt
     8281 catgatgact acatctgctg cttcaattgc agcatcgctt cccaatccac ccattgcgat
     8341 tcctacatct gctcttgcta gcaccggtgc atcgttgatt ccatctccga caaatgcggt
     8401 ctttttattg tttctttgta agatttcctc taaccgctgc actttatctt ctggaagcaa
     8461 ttcgctatat atcttgtcca cgcctacttg cttcccgata tgatcagcaa tttttttcga
     8521 atcgcccgtc aacatgactg tgttcttcac accagccttt tttaattgaa ccaatgcgtc
     8581 tacggcatct tctttcaagg tatctgcaat aacaagataa ccactgtaac tttgatcgat
     8641 cgctagataa agcaatgtac cgatttcttg acttgaagga aaagagattt caaatctttc
     8701 catcaatttg tgattacctg ctaatacttg tttgccatca acttcagcta agatcccttc
     8761 acctgcaaat tcttggatat tagctgttgg taaaagcgga cctgcgtagc cttctaaaac
     8821 agattgagca atcggatgtg tagaaaactg ttctgcactc gcaacatatt gcatgaatat
     8881 ctttggatcg atttttgtat cgatcgtctg gatcgaaaag tcacctttcg ttaatgtgcc
     8941 ggttttatcg aataccactg tctcagtttg tgcaagcaac tccaagtaat ttgaaccttt
     9001 tactaataca ccgattttac ttgctcctcc gataccgcca aagaaactaa ggggtacgga
     9061 gatcactaat gcacatggac atgagatcac taaaaatgtc aatgctcgat agatccattc
     9121 agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca gctaataaaa cggccaatcc
     9181 tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg aaattttcag ctggtgcttt
     9241 tttactgctg gcattttcta ctaattctaa taatttgctg atagtagaat tttcataaga
     9301 tttttctact tgaactaaaa atggtttatt tttattgata aatccactta atactgtttc
     9361 accaacatat acagatttag ggactgattc tcctgtaagt gctgacgtat cgactaatga
     9421 ttctccttcg atgatcgttc catctaacgg tacacgttca ccaggcttca caagtacaaa
     9481 ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat ccttccgtcg tctgcacgtg
     9541 agctgtttct ggacggatcg ccatcaattc gcgaatcgat tttcttgaat gactgactgc
     9601 caatccttgg aaatactcgc cgatttgata aaatagcatc actgctacgg cttctggata
     9661 ttcaccgatc aacatagcgc caatcgtagc aacagacata agaaaattct catcaaagat
     9721 ttttcctttg acgatattca tcactgcctt cttcactaca tcatatccta tcagcagata
     9781 aatcaataaa taccctatca agcgaattgg ttcattaaaa tcaaataata gcaataaagc
     9841 gatacctgca agggaaaata aaatccggaa cagcgtattt ctctcttttt catttccatg
     9901 ttcagatact ttttccttat ctatttctgt tactttgaca tctggttcta attccttcac
     9961 tacttttttt atttcttgtt caatcgtagc caaatgatct tcctctgcgt caatcgttag
    10021 ttttccagaa gtgaagttta ccgttgcttc cttgactcca ttgatcttct gaactccttt
    10081 ttcgattttc attgcacagt tcgcacaatc taaaccttct aatctgtaac ttttcatttt
    10141 aatgacctcc tcgcctcaac atatgaatat ctcttcataa ctaattataa atgaacggtt
    10201 attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca tctgcatcgg aaaatttctt
    10261 ccatacagat gttttggctt attctttctt tttcttttcg atcattgcca ctgctcttat
    10321 ccaacccgca cttgccttct tgatcttgac aggatagcaa ctaagtttga agcctgttgc
    10381 tggcaatttg tctaaattca ttaatttttc gataggacaa tatctttttt caatccctgc
    10441 aaaatgtcct tcccagatat tcgaagctcc actacgcttt caaaaatata gaaagagttc
    10501 tttaatgaaa attatttcaa ctttccataa acagtaaccc catataaata aaagacgtaa
    10561 aagtcacatg gaagaactta tacaggagaa cgcatagagt caggtgaaat acatgtatcc
    10621 cacctgactc tatgctttat ctaaccttta ttattctctt ttatacaatt tctgcaccga
    10681 atggcatcaa cgccaatcga gcaagtttga aagattgctt ggcaaaaggt atccctatga
    10741 tcgtgataca tagaaggatg ccgctgacga catgtgcgag agcaattggt aggcctgaaa
    10801 cgatcagcca gatgatatta acaatcaatg acatgccaga ctggtgataa attacttctt
    10861 tgccaaaagg cgcaaaagaa agggaggcga tcttaaaaca ttgcataccg attgggatac
    10921 ctatgatcgt gatacaccaa agtatccccg ctgctaacca gctaagacca ccgattagtc
    10981 cgccgaatat aaaccagatg acatttccta aacaactcat tgtatttctc cttctattac
    11041 tgtttttgat taaaactggg caaaaaatga ttttattctt ctttttttag cttccagtat
    11101 caaatattct gcaaattttt tttggatttc ttccgtattt tcttttgtta ctaatgtgtg
    11161 ttggattagt tttcctgatg gtcgctgatc caatcgttct tctgactgtt gcacgtccgc
    11221 ttgcgcaaat ccgataatcc gtttttcttt tgttacagca acgagcaata gtttattctt
    11281 atcatttagg tcagctgccg tttttgcttt tacgaatgat tctggatgat cagtaatcag
    11341 ctgattgatt tgaggtagat cttcttttac tgcccaccgt atttccatca tttctcactt
    11401 ttctttcgct catttttatc tccactaaaa aaatcgactg cggcaattgc atctatccag
    11461 ctttgtgcaa tgatctcatg tccgccaatc gttgggtgga ccccatcttc tcttgtatat
    11521 ttcgaaaaac cataaacaat ccctaaagca ttgaatatac catctaaagg aaccagcaat
    11581 gcgccatatt ccttagcaag tttacgaaca atttggattt tagggtctaa gtctttacgc
    11641 cagccatgac ggtcgtcggt tataggaagt acaaacggct ccatcaagat gatgttgcag
    11701 attcccactg tttgaagttc atttagtaat tttcgataaa cggattcaaa ttgctgagca
    11761 gatttctcag ttccgaatat agcctgatcg ctagtattat gccaggtatc attgattccg
    11821 atcaaaatcg atacatagtc aggttgtaaa gctaaacaat cttcttgcca gcgtacttgc
    11881 agatctgcaa ttttgtctcc tcctatccca cggttcatgc attcaaattc catcgttgga
    11941 tattgggaga ttagttttcc ggcaatcaaa agtggataac ctttacctag atcagttgct
    12001 tctgctcggt ctcttcctgc gtctgtaatg ctatcaccga taaataatat acgttttttc
    12061 atttgtcaaa aactcctttt tgaataaaac gatgattttc ttccttatat tatcccataa
    12121 gccctatcaa gacgaaagag aaaatatatg tataaaaaat gacaccagcc tagtcccttc
    12181 aaacaattga atgaacgctc atcaagtgtc tagtttttta tgctattttt ttcttaacgt
    12241 gccggattcc ccttgattcc ttcgttgttt ggtccagaga gatcgatcac acctaagctt
    12301 ccgtcaatca gaccactgat gacgccatca gaacatatat ttataggctc atctaagact
    12361 tctagtaaat gtccctttac taatatctct tctagattgc ttgggataga ttggtcataa
    12421 ctttatctgg tccgatgatt tcaagggtga attgaactta catatggatt attgcatgct
    12481 gaaaatacgg acctactcca tcttcgaatg ttactattag tttgttttca ggtgctagat
    12541 gctgctttat ttcaacagca gaagcatcat caaatcagat ttccatttga attaccttct
    12601 ttgtaaatgt ttgattgatt gcgatttcgt tatagaacct tgtgaatcaa atagcaattt
    12661 cttttgcttt cttctcttgt ctgctggaaa cttcctttat cccgtatttt aaaaaaagaa
    12721 ctgcgagagt tcgcagttct tttttagaat aaacggtgct ctccggccaa ctcctcggtc
    12781 acaagtcaca atagtcatca aacatgaaaa actgtttttt gaaattactc cttgtgactc
    12841 ggagctgaac ggcaggcgca caacctcttg atataaacgg tgttcaatca gctgaccctc
    12901 gatattagct aaaaaaacgg taaaatatga gaagctattt ttccgttttg tttctctaat
    12961 actcaggtca aggcactgat ttcacaacct cttccttaaa cggtgttcaa aagccagttt
    13021 ctcggtatta agccaaattt atgcaaaaat gtggaaagcc atttccacaa aatttttctt
    13081 aatactcgaa actaaacagc tttttcacaa cctctccact aaacggtatt caaaagctta
    13141 catctgcggt aataagtccc gccaattcaa aaacatgaac agctgttttt gcttggctgt
    13201 tctaattact tgatgcagaa cagctttttc atcacctctt atggataaat tcggttcaac
    13261 aaacgtggga atggaatagc ttcacgcaca tggtcgatac cagcaatcca agtgactgtt
    13321 cgttcaaggc ctagaccaaa accagagtgc ggaacagaac cgtatttacg tagatcaaga
    13381 taccatgaat attctttttc atccaaaccg tgattacgga tttgttctag caagaaatca
    13441 tagtccgttg cccgttcgct tccgccaata atttcaccgt aaccttctgg tgcgatcata
    13501 tctgcacaaa ttaccacgtc ttctcttgtt ggatgtggtt tcatgtagaa tggcttgata
    13561 gcttttggat agtttaggat aaagacaggc tgttcgaatg aattcgcaat gaacgtttca
    13621 tgaggtgaac caaagtcttc gccccattcg atgtcatcaa atccattttt cttcagtaat
    13681 tcgatcgcat catcataagt aatacgaggg aatggcaatt ttgtataagt ttttaagact
    13741 tctttgtctc gtcctagtac atctaatgcg tattcgcaat gatctaaaac attttgtact
    13801 aagaatgcta catattgttc ctgtacttct aaactttctt cttgatgagt gaaagccatt
    13861 tctggttcga tcatccagaa ttcaatcaaa tgacgtcgtg tttttgattt ttcagcacgg
    13921 aaagtcggac cgaatgaaaa gacttttccg aaagccatag cagcagcttc catgtataat
    13981 tggccacttt gagataaata agctttttga tcaaagtaat ttgtttcgaa tagatccgta
    14041 gtgccttctg gtgcggaacc agtcaagatc ggcggatcga ttttgacaaa atgatgttga
    14101 ttaaagaatt catacgttgc acgaatgatt tcatttctga tctgcataat cgcatgttgt
    14161 cttgaagaac gtagccataa atgacgatga tccatcaaga agtctgttcc gtgttctttt
    14221 ggtgtgatcg gataatcgtg gctctcgctt accacttcca catttgtaat tccgatttca
    14281 taaccaaatt ttgaacggct gtcttcacgg atttctccag ttactaacac ggatgtttct
    14341 tggtgcagat ttttagctgt ttggaaaact tcttccggca cttcgctttt tacaactaca
    14401 ccttggaaaa aggctgttcc gtctcttaat tgtaaaaatg cgatttttcc gcttgatcgc
    14461 ttatttgcga cccaagcgcc gattgttact gtttctccca catggttttt cgaatcgatg
    14521 atttgaattt gttccacgta tgactctcct tttatattcc ttgaatttct tcaggattaa
    14581 ttttgacttt tactctcaat aaattgcttg attctgcgta ccgcttcttc taacgtagcg
    14641 cgatcagtgg cataactcat gcggacattt tctggtgccc caaagccttc gccagtcaca
    14701 agcgctaccc ctgtttcttc taaaagatct tctacccatt gtgtgacatt ttcatattta
    14761 cacatgcgta gcgtttcttt tacattagga aaaagataaa aagctccttg tggcttttca
    14821 agagaaacac ccggcagttc tgccacaagc ggatataacg tattcaggcg ttcttcaaag
    14881 gcgttccgca tttcttccac tgtatcttgc tcgcctttca atgcttcaac agctgcatat
    14941 tgactgactg ctgtcggatt gctagttgat tgggaagcaa cagcgatcat tccattgatg
    15001 atcacaggat ttcccaccgc ataaccaatg cgccagcctg tcatcgcata tgttttagac
    15061 acgccgttga tgatgatggt ctgatttttg attgcttctg aaatcgttgc aattggagta
    15121 aactcattgc cattataaac taaacggcca taaatatcat cagcaactat caaaatatca
    15181 ttttttactg cccattcacc gattgcctgc agttcattct tgctataaat cattccagtc
    15241 ggattcgatg gagaattgat gattacggct tttgtttttt ctgaacgtgc ttgttctagc
    15301 tgctcaacag taactttcca gctattggat tcttctcctt tcaaaaagac aggaagtcct
    15361 tctgccagtt ttacttgctc accgtaactg acccagtaag gcacaggaat gatgacttca
    15421 tcttgcgggt caaggatcgt ttggaaaagg gtatacaaag caaatttcgc tccgtctgtt
    15481 acgactgttt gtgaaggttc atagacaagt ccatagtttt tcttcagata atcaacgatt
    15541 gcctgtcgaa gctctgggat tcctgctgtt ggtgtataat agctagcttt cccattgcgg
    15601 attgcttcga ttgctgcttc ttggatgttt tctggtgtcg caaaatccgg ttctcccaca
    15661 gtcaaactca gcacatctct gcctttggct tttaatgcct tggcctttgc cgcagctgcc
    15721 aaagtgacag acggttctaa tcgttttgtt ctttttgata actccatccc attcattcct
    15781 ttacctttta aattcccttg attgtcttga cttcatctcc agattcaaag ccaatcaggt
    15841 aataattcag tttcccattt gtttcttttc ctactacttc ccacacaggc tgatcatcat
    15901 acatccctaa agcagctttt tcgatttgga tttcaggatg ggccgcagca attgtctgtt
    15961 ttgcttcatt ttcagtcagt ccctttgttt gatccaatac ttttactttt tcaccagatt
    16021 taggaatgat aacggcgatt tcttttcctt tatcatttgt acctgtcagt gaaaagtatg
    16081 tcttctttcg agtaaaccaa taaaaatgat cggcttcctc aagatttgca tatttctttg
    16141 cgatttctac tgcttctttc ttcgcctgca ccgccggacg actggaacgc aagtaaaaaa
    16201 tcgagctgat cacgatgacc gctaataaaa atacgatcaa gccaagtaac acgcgattaa
    16261 tcgttttttc ctttgtctgt tttttcttca agggtcgtcg ctcctttatc ttcattcctt
    16321 ttcattatag cagattttaa ttatgaaaaa agacagctcc tctaacttcc ttcttcattt
    16381 ttctttaaga attcatggat atcctgatag atttcctcca tagctagctc tttgatcggt
    16441 acttttttcg gtaatgcatg gatgatccgt gcaccatatt ttgcagtaac tagtcttcga
    16501 tccaaaataa gcaatacgcc acgatctgtc tccgtcctta tcaagcgtcc taatgcttgg
    16561 cgtaacttca aagctgcttt aggtacggat tcctggtaaa aaggattgat cccctctgat
    16621 tctaaataag cgttcctagc tttgaccatc gggcgaagcg gattatcgaa gggtaaacgt
    16681 gtgactacaa cgatctgcaa agcatctcca ggcaaatcta caccttccca aaaactgtca
    16741 gcgccaaaca atacgctttt ttctgacaaa atgaagcgtt tcaaaagctt ctcccggctt
    16801 ccgccaaacc cttgagctag tatttctcgt cctttttcta gcatagacag atgcattttc
    16861 tgatagacct tctgcaaaac atcatgagaa gtgaataaca caagtattgg ccgatcttct
    16921 tgttcgatca gacctgtaag tgctgtctct aaataatcga tatatgcttg attgctgact
    16981 tgatggacgg caggtgcctc ttttggcaca tagatccttg cctgtttctc ataatcataa
    17041 ggagaggcaa tcactttaag aggtgctgct tcaatgccaa gccgcttaga gagatagtgt
    17101 cgattagggc cgatcttcag cgttcctcca atataaagaa tacgatcata tcgaggatac
    17161 caaacagtcg acggcaacat agccgcttga aaatcatgga gatgaaaaat catctgctgc
    17221 gctgtgttcc caggaacgaa ccaatggatc aaatgatctc gccagtcttc cagccattga
    17281 tccatcagca agtactgctt gtctaattga tcaaataagc tatataaact tgctaaatcg
    17341 atccgcatct ggtttgtcca gctttcttgg ataatctcaa aagtcagacg catttgtgac
    17401 tgtagctgct ggatctcctg ataaaaaagc tgcaggtgat caatggctgt cccagattcc
    17461 agtggcagct gatccaacat ctcttttgtc gtcatgactt cttctggata ctgtccgtta
    17521 ggttgcttgc cgctcactaa actcattaat ccttctgcca tacaaatctg tgcttcgcat
    17581 agcgcagaaa gttcttctcg ataaatcgat aaccagcgta aggcttcatg ttctttcggt
    17641 aaatagcttt gtattcggtc aaacagctga tttggttctt ctaattgctg gatttgttta
    17701 tggaaatagt taatacttaa tcgtttgtca tttacttttt cagcgatttc cggcaaatga
    17761 tgtacttcat cgatcaacaa aaatggacta gctggaagca atggttcttt tcgctgtgtt
    17821 tcttgtatta aaaacgcatg attgacaatc aaaaaattac tttgcctgat tttcttttgc
    17881 aagaaacgaa caaagtcttg ttcgtaaaat gactgatttc ttgctaaaaa tccagtgcct
    17941 ctgtgcgcga tatctgcaaa gaatgaatgg ttcaaatttg ttaaatgtaa ttcatccaaa
    18001 tcaccggtct tcgtttgcgt cagccaaact aagaccgcca tttgatacaa agcatattgt
    18061 ttttgttctg gcggttccac aagtgtgcct ttaaaacgct gtaaatcaat atagtggcga
    18121 tagcttttga taattacagg ttgtatgggc tgatctagca attgattcag caatggaatg
    18181 tctttttcta tgatctgctg ctgcaaaact agcgaaacgg tactgatgac agcgggtttt
    18241 tcaggtgtag ccaaataact caatggcagc agataaccga ttgttttacc cattcctgta
    18301 gctgcttcga tcatcaaatc ttttgattca tcttttgtga aatggtcgta gaccgcattc
    18361 atcagtctgt tctgttcttt tcgatacatc agtgttttac caaacatttt ttcttttgct
    18421 tgtttttttc ttggatatgt ccgctcgcca taatggacgt atatgaatag ttccatttct
    18481 tttttctgca aagctagccc atcgacgatt tctaatgagg gatcaagtgg ctctggtttt
    18541 tctttcattt cttcaatcac atgataaatg aaacgactcg tatccatacc tgtgtgtcta
    18601 cttaatcgcg caatctgctc taacgtgatg attggcaatt ttctcatccg ttcttcgatc
    18661 agcaaaagaa gttgacccgt cacttcggca tcactatctg cctgatgagg attctcatga
    18721 ataaatccaa ggctttctga caaatcagct aagcgaaagc ttggttccgt cggcaagaaa
    18781 atttgtgcaa gttctactgt atcgatgcct ggtatcttca aactaggaag tccgcatcgc
    18841 atgagttcat ggttcaaaaa attataatca aaataaatat tatgcgcgac aaatatggtg
    18901 tctgccaaaa gattgtaaat cgtttgggcg acatcttcga aataaggcgc tttttggacg
    18961 cgttgattcg tgatccctgt taaatgctgg atttgttttg aaatcttccg ccctggatga
    19021 atatcgatcg aaaaacgtga agtgatccga cctgattcaa tcatgacgca gccaaattgg
    19081 atgatccgat cttcttttgg attcgtccca gtcgtttcga tatcaacgac tgcataagtt
    19141 cttggtttca aaatatcttc ctcttctcct gcctctaatg ttcttttgaa attatactac
    19201 aaagtgcaga aaaatactct catcgctttt taaagaaaat agacaaaaac gcccagacac
    19261 gcaaatgtct gggcgtttat tttttagtat aggttaatta agctttagta acgtttgttg
    19321 cttgaggtcc gcgttggcct tcttcaacat cgaaagttac tgcttggcct tcttctaaag
    19381 ttttgaatcc gtcgccttgg atagctgaga aatgaacgaa tacatcgttt ccgttttctg
    19441 cagtgatgaa tccaaatcct ttttctgagt taaaccattt tactgtacct gtttccataa
    19501 taaaaatcct ccttgtgtat caacacatat tttgcaatta cttgaatggt gtatatctgg
    19561 aagatgttta tttgaaacaa gctaccacag attaccgaac aaaaactact cctatagtat
    19621 atcataccgt ttacaaaaaa gctagagttt ttcgactttt tttattaaaa aaagttttct
    19681 cagcgattac ataaagtgaa aaaccgaact ttttttaaaa taaacgacat attgttcagt
    19741 tggtgttttt ttgctctgat gttcttcttt aaactaaaaa aattattctt tctgactact
    19801 tatcggttct gtttcaacgg tcgaggctgg ggaagattct ttagtaggtc tgatgttctc
    19861 atggtatttc tgatagccta tgacagttac cagaagaaca aggcaaacca atacgatcaa
    19921 cgagattagt ttttcttttt tgctcatatc tatacctcat aaataacagg atgatgtcca
    19981 tcatatttct gagtatgagt aaaagcaaaa gctgcagctg tagaatgtaa gaaatgccag
    20041 caactttcat aatcacaggt cggacagtac caaaagtaac caaatcttgg ctgcaaagac
    20101 cgttcttctg tttcaggaat cgcagaatca agatctgttg tctgtacata aacatttttg
    20161 tacagacgtg tgcctgtaat aggagaccgc tgtgtttctt cagcaaaaac tggctgactt
    20221 gttaaactta gggatagaaa cagcaaacac cataacagtc tttttttcat aaagacctcc
    20281 ttatcgataa gtttttccca attaaaatca tatctcaaga acgcccgtta ttcacatttt
    20341 gttcataaat gatgtttttt gtatcataat cgtcaaaaag aggtcgtgac cacgtcacaa
    20401 cctctttttt aaacggtgtt ccagaagtag cttcttcgga aataagtcga aatttcacaa
    20461 aaatttgaga aataattttt gtcaatctct tcttatttct tgaagctgac cacttctgtc
    20521 acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtattagc tcaaaaaacg
    20581 gaaaaatatg tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat
    20641 ttcacaacct cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt
    20701 tttgatccaa aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca
    20761 acacttgacg ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg
    20821 acgtagcgat aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta
    20881 actgatcgat gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga
    20941 gttcacttga cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag
    21001 aagcagaaat cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg
    21061 tctgattttc tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat
    21121 ccttcaagta cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat
    21181 cttttggatt tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag
    21241 agaagttcaa accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg
    21301 ccccttctgg tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg
    21361 agtcgcggtt caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag
    21421 ccagtaaata aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat
    21481 ggatataggt ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa
    21541 acgaaggatc tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg
    21601 aatatttttt ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat
    21661 ctttttccac atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt
    21721 tagcgccttc tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac
    21781 ccctcctaaa aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc
    21841 atacaataaa acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt
    21901 ttttaagtta tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat
    21961 gaaatgctga taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga
    22021 ttttcagttt attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg
    22081 cggaaacaca gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc
    22141 gcgttcttat cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat
    22201 tttaatttgt agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca
    22261 cgtctagtcc tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt
    22321 gctgtgcaag ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg
    22381 caaagcggta agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc
    22441 acaaaaatca tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg
    22501 gatgataaag tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg
    22561 aatgttcctt tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc
    22621 atccagtcag ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct
    22681 aaattcactg aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc
    22741 gttaatgcat caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga
    22801 gaaaacgacg aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc
    22861 ttggatttcc cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat
    22921 acgcaatcac cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac
    22981 aaggaaggcg tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt
    23041 ggtacgatca ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat
    23101 gcggaaaacc cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac
    23161 aattggtcag aaaaagagct aggaaaagct aacgcaagta ttcctagtct aaaaaccatt
    23221 gatgtaaaag acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta
    23281 tgggtacgaa acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag
    23341 caagtcactg acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct
    23401 acgatcacct tggcagacga taaattaggc tatttagaag acaacaatgc accttcaaca
    23461 gagatcatta cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc
    23521 gtcgcagaat ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg
    23581 gaaagatgtt tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta
    23641 tactgtataa gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt
    23701 tctctatctt attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc
    23761 cttgttcttt tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt
    23821 ggaaattttt tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc
    23881 tttttcttat ttcacagaaa aacgaaggct gctgaatggt cttttattca atgttttcct
    23941 catcagtttc ggcatctact tgtttgtctt gctgtatgaa acgcaaaata ttttccttgg
    24001 cggactgatc gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg
    24061 attgattgtt tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt
    24121 agctaatctt ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt
    24181 tttcttgtta aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat
    24241 cttgatctat ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt
    24301 caaccggcct cgatacaatc aagacttcat cgttgtgtta ggcgctgggt tgatcaatgg
    24361 agaaacagtt tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc
    24421 tcaaagccgt gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga
    24481 cgaaaaagta ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga
    24541 aagagacatt cttgtcgaaa ccaattcgac aaccactttg gagaacatgc tctattcaaa
    24601 ggaaatcatg gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta
    24661 ccacattttt agagccggtc tttatgcgtg gcaagccaaa ttgaaagcaa atggtattgg
    24721 tgcaaaaaca gctttctact acttgcctaa tgcgttttta agagaataca tcgccatctt
    24781 gatgatgaac aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt
    24841 cttatcgctg attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac
    24901 cttgtttttt tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc
    24961 tacttgctga gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat
    25021 cataaagaag tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc
    25081 aatcggtgta tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg
    25141 aacagaaaca tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa
    25201 tggtgtcact tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt
    25261 cccaataaaa tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact
    25321 caatccgaga taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc
    25381 tatcgtattt cgccaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac
    25441 gatcgaacgg ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt
    25501 gaagaacagc aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac
    25561 accatagctt tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc
    25621 ttctgttcgg actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg
    25681 tgtgccaaat acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc
    25741 tgatttatcc aatcggaaca aacgtgctgt attatctgct aacgctcccc ccatttggac
    25801 tgctgttcct tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag
    25861 tgtaagtgga ataagtcgca acgggatttt ttcttctcca ccatttcctt gatcgatcac
    25921 taagttgttt ccacgactag aaagaccgcc gtgatacgca tagagataag caaaaagcgc
    25981 accactaact ggtagtagat aaaggagcca aggatgtgat agtcggatat ctgtcacaac
    26041 tgtcaggcta tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt
    26101 gatcaccagc cacttcagca tgtacactaa aatgcgtact ggactcggta attgtttttc
    26161 cataaaaaat tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact
    26221 ttagtaggcg tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc
    26281 acttccatta aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt
    26341 tacacgttca gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc
    26401 actatcatga caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt
    26461 tgtagaaaga aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt
    26521 tatcgttggt gataaagtac cactaatccc ttttaatttt tcatcttcgt aatagatcat
    26581 gaattttttc tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat
    26641 ttcattccaa gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg
    26701 tttgctaggc tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctcgttctgc
    26761 ttgcgctttt gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga tttctggcag
    26821 ttcgtgttct aatatgtctc gaatcttttc agttgctagt gcctgaactg tttgtaccgt
    26881 ttttggatca ttgacagatg agtacgcatt taataactgt tctacttctc tttttataaa
    26941 aaaatattgg tatggtttta ttgtttgttc catgatttta aagtcctttc ttctaaccaa
    27001 agtgcatact ttgtagaaaa aagtgatctt ctgcaaagta tgtgttacct gaagatcccg
    27061 gactctttta aataagatac gttttgcatc ttatcatcac cccctttaat gcttattctt
    27121 ctatgtacat gattcattcg ataaataaaa gtaaaccgta tcttgaacag atagtcaact
    27181 gcaaacagaa aaaaggactg tgacaaaagt cgtgtcaaag tcccttgttt cgaatgaatg
    27241 gtgttcaaaa gcttgcatct gcggtaataa gccgaaactt cacaaaaatt tgagaagtaa
    27301 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcaatatgg
    27361 tctattattg gttttacctg ttaaatcgag gattctttcc caaagtaacg ttgccatctc
    27421 ttacgcatac ttgcagacat gggctgagcc gcttctttga ctgcgcagta tttgtccacg
    27481 aatgtcacaa tatatccttc tttatactta ggcggtgcga tcgttgcacc ccacatgtgc
    27541 ttcaaaatga tatctcgctc aagatccgac aaatcggtga ttttttctgc attctttaca
    27601 gcaattcttg gatggatata cgcatgtgtc ccctcgtcaa atttcgtcgt acgccaatca
    27661 tagtagaaca gatcatgcaa aagtccagca cgcgctgttg ctttagcatt gccaccccat
    27721 ttttttgcga gcagataact atagtaagaa acactgatgg aatgctccaa acgagtggaa
    27781 tgaacatgct gtgtgtaatt cgctaatttt ttcactgctt ctgtttctaa aagatcttgt
    27841 acataagaca tatattcttc gtcttcacgc catttttctg tcataattat cgatctgcca
    27901 cctttttgaa tttcattttc ttctgaaatt gatctaagta taccaccagt gttcggatta
    27961 ttctagtcgc tctagataaa atagctaaag cttaatagtt ccttaatcat aaagaaaaat
    28021 gataaagaac gatccctgct gcgactccga cattcaatga ttccgcattt cctttcattg
    28081 ggatatatac gttctgatca gtcaaagaca agatttcctg acttactccc tgaccttcgt
    28141 ttcccatgat tatagcgaaa tcctctgttt tttctacttt gtctaatgca tgtgcttctt
    28201 catttaattc tgtaccgtac accgtaaacc ctttgccttt cattacaggg atcacttctg
    28261 ccaaatcttt ttgtaacaca ggcaaatgat aattgcttcc ctgcatgctt cgcagcacct
    28321 ttgtactata aatatctgcc gttcctttac ctaataaaac accagccata ccaaacgcat
    28381 ctgccgtacg gatcatcgtt cccacatttc caggatcctg tacattatct agtaaaagcc
    28441 agcctcctga aaaatctggc gtatcttcag atgttaacgt catcaccgct aagatccctt
    28501 gcggtgtagg tagatctgac atagcggtca tgacctcatc tgaaactaga aaagtagaaa
    28561 tctctttgtt tacagccagc cagtctcccc attctttcat acctctctgt gtcacaaaaa
    28621 gttcttttat ttctgcatca gacgacgcag cttcttcgat cagatggaac ccttccaaaa
    28681 gatattctgc cttttcttct ctgtatttct ttttatgtag cttttttaat tctttgattt
    28741 ttggattttt tgctgattgg atctctttca cattccttca cctcacctga ttatatcatg
    28801 gaataggtgc ttgagcttgt ttcatcctaa aaaaatcggt atcattgatt tataaaacag
    28861 gaaagaaagg tgacgaacat gcgcaaagtt aaaatgaatg tccaaggacg tgtccagggc
    28921 gtgggatttc gctatatgac caagatggtt gctgatcagt taggcgtgac cggaacggta
    28981 aaaaacgaag acgacggctc agtcacaatc gaagcaatcg gaaatgacga catcatccaa
    29041 aaatttatcg aagaagtcaa gaagtctccg agcccaagtg gacgagtgca gtatgtggat
    29101 gtccaagaag atccgatgat tgaggaacga aagaaatttg atgtggtggg ataaggttgt
    29161 gaaaaggtcg cttagttttg agtattaagg aaaattttgt gaaaatggct ttccacattt
    29221 ttgcataaat ttgacttaat accgagaaac tgacttttga acaccgtcga tcaggttgtt
    29281 tttgaggcgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat ctataacttg
    29341 caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta gttttttata
    29401 taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct tgattttttg
    29461 tcttttttca aaatttttcg ctacgaaaaa gaaagaatat ttatatgaaa tttctatgaa
    29521 gttgattgaa ttccatgcgc ttttttagta tagttaagtt tgtgtaaaaa ttatagaaga
    29581 ggaagtattc tatgaataaa tggaaaaact ggttattggg atccggactt gtcagtatcc
    29641 tgctcttttt atctggctgt gtgcggatgg attcaaacgg aaaccctgac acttcaggaa
    29701 tcgtttatcg agtactcgtc catcctatgg gccaagcgat tacatatcta gttgagaatt
    29761 tcaattggtc ttacggctgg gcagttattg caatgactgt gatcgttcgt atcatcatct
    29821 tgccactagg tatcagtcaa tcgaaaaaaa caatgatcca atctgaaaaa atgcaagcgt
    29881 taaaaccaca agttgaagca gcacaacaaa agctaaaagc tgcaacaaca cgtgaagaac
    29941 aaatggcagc acaagcagaa atgcagcaag tatatcacga aaacggattg agtatgaccg
    30001 gcggaatcgg ctgtctgcct ctattgatcc aaatgccgat cttctctgcc ttgtatttta
    30061 cggctcgcta cacagaaggt atccgtgaat catcttttta cggcatcgat ctaggctcac
    30121 caagtatggt gcttgtggcc attgcagggg tcgcttatct gcttcaagga tatatttcaa
    30181 caatcggtat tcctgaagaa cagaaaaaga cgatgcgtac gatgctgatc gtctctcctg
    30241 ctatgatcgt cttcatgtcg atcagtgcgc cagccggcgt tacactttac tgggtagtcg
    30301 gtggtgtgtt cagctgtctg caaacattca tcacaaacgt tatgatgaaa ccacgcttga
    30361 aagcacaagt ggcagaagag ttgaaacaaa acccgccaaa acaagtcgtc actccaagaa
    30421 aagacgtgac agaatcagca aaaccagctg acaacaaaca agcaacaaaa caaatcaaac
    30481 aaccaaaaaa cagtacaaat aacaaaggcc gcaacgcagg aaagcagcag aagcggaagt
    30541 agatagtaaa taagagtgta ataaaaattc attaacagtt ttagcaatta aaaatatcga
    30601 ccaagatttt tgaaaaagaa atctagtcga tatttttatt taagtgaatg tataatttga
    30661 atcatcgatt gatcgctatc taattgcagc agcatctttt tatgaagaaa caactttttc
    30721 ttatctatca acgtagacag gtgttgctgt ttcttttaat agtgtactta attccttaat
    30781 attttgtttt ccttcttcta acatccaact actcgctttt tcttcgcctt cggtaacaaa
    30841 aggtgccact cccttcgccc atgttgctcg cccacataag acaccattga atgtcgaacc
    30901 tgattgtttt gcaaaacgta atgtatcttg aaataacgta gcattgactc ccgcacttaa
    30961 aaaaataaac ggttgttcag tcactttcga ttgttcatag aaatacgcgg atgcctctgc
    31021 ttgactataa caagtctctt gcccatatcc ttccacaaat ctcatattca ctggcacttc
    31081 tactttcaat acatccactt gataacgttc ttttgaaaat tctcgcatgg cttcaataac
    31141 tttatggggt tttacttttg cataggcaag agaggacgga tcgccaatag ttgcatcata
    31201 agaaaggatc tctaaaaaga aaggtatgcc ttctgcatcg cattctgaac caatccgttc
    31261 tataactatt tgtttttgca cattaatctc tgctggttca tccacgtcat agtacaataa
    31321 aaatttacaa gcatccgcac cagcttcttt taaccttttc accgaccaaa tattcaataa
    31381 atcaggtaat cttcccggca ttttcgtatc atagccggtt ttttcataag ctagcagcaa
    31441 gccacattct ttctctctaa gcgctgctgc aggcaggccg cattctggat caagtaaaat
    31501 agctgaggca tagggagtca ggtattgaga aaccatctgt ttaaattgga tgatgtcttc
    31561 tgatgtagct tctttatatt ggttgatcat tttcttcaaa gcaccacgct ggtcgattgc
    31621 taacgcgcta ataattccag cttcatccgt caatcgcttc aaacgttcct gcttttctat
    31681 actcatcttc ttcatagatc ttcacctatt tctttagtta atattgtggc tatttctgtt
    31741 tttgattttg cttttctcaa ctgttctgta acctcttttc gcatcaatga tcgtgcaacg
    31801 agagacaata atttcaaatg agtcgttcct gcttctgaat ctggaataaa taacgcaaaa
    31861 ataaaatcaa ctgactgtct atctaaactg ttccattcaa ttcctttgtt cagtgtgacg
    31921 ataatcatac taggttcgtt gattgtttga gacttcgcat gagggatagc aaaacctgac
    31981 attattcctg tagtaccttc tttttctctt tctagaagtt ttcgatatac ttctttttcg
    32041 tcatgagcga ttcctaaaca gaatgtttgc tttgctaaaa acttaaatgc ttcttcttga
    32101 ctatctaatg gctgttgtaa aaaaatgttc tcttcatgaa tgattgtcat gataagcctc
    32161 ctgctcttcc ttcttcgata actgtacgat ttgctgcttt attatccatc ttcttgtctt
    32221 tatcagttac tcttttcttt tggatcaaac ctagtatcac tccaccaacg atcgaaccaa
    32281 taagaatcgc tgtaacccat tggatttttc ccgtcactac aggtaacact aaaaaaccgc
    32341 catgtggggc tggtacttgg acaccaaaca gataggtcaa gacagcagca attgatgagc
    32401 ctaacatcat cgttggtaat actcggatcg ggtctttcgc agcaaacgga atagctccct
    32461 ccgtaatatg agttgatcct aaaataaaat taaccaaacc tgcattacga tcattagtat
    32521 caaaatattt accaaacaat aaagttgcaa aaccagtaat caacggtggt gcaatgcaag
    32581 ctactgacac gccagccata aaagtcgtgt ttccttgtgc caaaagagct gtacctgtta
    32641 catatgccgc cttattaatc ggtcctccca tatcaaacgc acacatgcaa ccaacaatca
    32701 cgccaagaac aagtggattc gaatgttcaa atccagctaa aaagctcatc attccttcat
    32761 ttatagcctt cattggaccc gccagcaaag acatcacgat gccgataaac actacaccaa
    32821 taactggata taagaagatt gccttcaagc catctagttg tttaggtaaa attgtcagta
    32881 gcttttgcaa aaataagata aaataacctg ctaagaaccc agctaaaata cctactaaaa
    32941 atccagtacc gccactcatg gccacgattc caccaacaaa tccaacgact aaccctggac
    33001 gtttcgcgat cccttctgca atataagctg ctaaaactgg aaccatcatc cccattgctg
    33061 catcgccaac cgtttttaac atcgccgctg tagcattgta ttgtgcatgg tctggatcaa
    33121 aagaatagat tccccaaacg gcaaatgaaa tagcaatcaa tactccgcca gctaccacga
    33181 aaggcaacat atgagagacg ccattcatta aatttttata gatgctgcga ccaaaattag
    33241 caccacttat cgtttctgat tctaaagtat caacagcttc attatctgct aagatggtcc
    33301 cttttcctgc caatgcttcg tcaatcagcc gttgtgcatc cttgattcct gtactgacag
    33361 aaacatcaat gactcgtttt cccgcaaatc tttctgaatg gacatctttg tctgcggcaa
    33421 taatgacagc atctgcctgt ttgatttctt cagccgttaa ttcattctcg atcccaattt
    33481 gtccatgggt ctccactttg atcgtgatgc ctttttttct agctgcttgc tctaatgcct
    33541 cttgcgccat gtaggtgtgt gcaattcctg tcggacaacc ggttgctgca attagttgat
    33601 attttcccat ctttcgaacc tctcttcctt gattttgatt tgtttactta attcctccac
    33661 atccaaaaaa tcagtcagtc ctttacgaaa agcagtagag ctgcctgctg ctatgctttt
    33721 acgtaacgtt tcatcaagcg gtcgtcgatt catgtatcct actaaaaaag tgcctaacat
    33781 ggcatcacct gcacatgctg tattgacaac ttttcctttt ggcgagtttc ctcgaatcac
    33841 cctgtctttc gtaaaaagaa tcgctccttt ctcaccaagg gataacaaaa cattttctgc
    33901 acctgactct accagtcgtt gtccataaac aaagtaatct tcatctgtaa ccatttttcg
    33961 tccgaaccaa gaagaaagtt cttcctcatt cggtttcagc aaaaatggtc gatagggcaa
    34021 acaatccatg acctcttgat cactgctatc aataatcaaa tgaatctcct tttcctgaca
    34081 aatccgactg atttccacta aaaccttcgg agacattcct cttggtaaac tcccagaaac
    34141 acaaagataa tcctctgctt gtaatgctct tatttgattt aaaaaattat ggacagctgc
    34201 ctgcggtatt tcaggtcctt gattcactaa tttgtattct tctctcgttt gattgacttg
    34261 tgtaaataca ttgatacgcg tcataccagg cacttcaata aactctgttt ggatttgttt
    34321 cttctgtaaa tagtcttcta tatattttcc tgtgaaacca gcagaaaaac ctaatgccgt
    34381 acttgggaaa tctagcatct ttaatactag tgacacattt actccttttc cgtttgcctg
    34441 aatgtcatcc tcctttgtac ggtttaccat aaaaggatgc atctcctctg tttcaataaa
    34501 tagatcaatt gccaaattca tcgtgcatgt gtagatagac ataaccaatg acctccttga
    34561 atctattatg aaatacgcga aagagaaaat gttttcttaa tcgttccaac acaaagaaaa
    34621 cctttacatt tataaacaca tcgtgctatt ttttaactaa cttttagtga aagaaggaaa
    34681 gaatatggat cacaaattaa gtgaaacaga acagtacctc tggaacttta tcgaacacca
    34741 catcctagag atccctaact actccatcgt gaaactaagt gaacaagcaa atgtctcaac
    34801 agccaccatt gtgcggacaa tgaagaaaaa aggctatgaa ggatttactt cgtttaagca
    34861 ccatctaaaa gaaggagaaa acaagaacat caatttttct ttttagataa agtagacaaa
    34921 ggtatccgtc gagccattct aaaaaatgaa caagaagtgg tacgtacgat caatatgttg
    34981 gaaatcggga acattgaaga tgcaatacaa agaatcaaat ttactgaccg ggtttttgtc
    35041 tttgcccgag gattttcaga aatgattggt caagagatgc tcgtaaaact tcaattaaca
    35101 ggaaaaaatt gtcaaatgca tactgatcca gagattatca aaagcatcag tcggaaacta
    35161 actaagaaag acagcgttat ttttgtttct ttgaacggcg agacaaaaga actcgtcacc
    35221 gccgcaaaga actgctatga tgcagagatt ggttcgattc tagttacagc caatcattcc
    35281 tctacactca aagattattg cgagataaat ctcgtaggtt tcaaatcaga aggttcttat
    35341 ttccctgatt atgaagtacg ttcacgcttg cctctccaga tcatcgcgag aatcctgctt
    35401 gacgcctatg tattgcgtat gagggagaat tgagaagcag tttattatgg ttggttacag
    35461 tttaaaacat cacatttttt attctttata ttgtatggac tcacgtaaac tttttatgta
    35521 taaagttatt cacaatctat aaagttattt taagtaacaa aaaataattg aaaatactta
    35581 aaatcaaaaa atatcttttt aatcaataaa aaaattgaca gttttattaa taataagcta
    35641 ttctgttata gaagaaaaaa ttgtttgtta ttattagcaa caaaaaaagg agtgcatcat
    35701 gaaaaagtta tctcaaaaac gtttagctca aatcattatt gattctagaa aatctctgaa
    35761 aatgacacaa caacaacttg cagtagctac tggtatcaac cgtgccttaa ttagtcgagt
    35821 agaatctctc gattttatcc cgtcaataca acaattagaa tcattagcag aagttctaaa
    35881 ttttgaacca acagatctat ttataaatga atcaaataaa tcaaacagaa caaaacatca
    35941 gtcgaagaat attgctgtag ccggtactgg ttacgtaggt ctttccattg ctactcttct
    36001 tgcccagcac aatcaggtaa cagcagtaga cattcttcct gaaaaagtaa aacaaattaa
    36061 caataagaaa tctccaatac aagacgatta cattgaaaaa tatctagctg aaaaaaatct
    36121 taatttaatt gcaacgactg atggtgcagc cgcctataga gaagccgatt ttgttgtcat
    36181 tgctgctcca acaaattatg atagtaaaca aaattatttt gatacttctg ctgtagagtc
    36241 agtaatcaaa ttggtgttag aagtcaatcc aaaggcaata atggtcatca agtcgacggt
    36301 acccgtaggc tataccgaaa gtattcgtaa aaaatacaat actaaaaata ttctattcag
    36361 cccagaattt ctcagagaat ctaaggcatt atatgacaat ctatatcctt cgcgaattat
    36421 tgtcggcacg gaccaaactg acaaaacttt acgagaagcc tctcacactt ttgcagattt
    36481 actccaagaa ggagcaatca aaaaagatat cgaaactctt ttcatgggat tcacagaagc
    36541 tgaagcagtt aaattattcg ctaatactta ccttgcactt cgagtatctt acttcaatga
    36601 gctggatact tatgcagaaa tgaaaggact aaatacacaa gaaatcattc acggggtttg
    36661 cttagatcca agaattggca cacattataa taatccatct tttggatatg gaggctattg
    36721 tttaccgaaa gatacaaaac aacttctcac taactataac gatgttcctc agaatatgat
    36781 aagtgcaatt gtcgatagta atcgtacaag aaaagacttt atagcagatc gagtgttaga
    36841 acttgctggc acgtatgagg ctaacagttc gtggaatgca gaaaaagaaa aagaggtagt
    36901 cataggtgta taccgtctta caatgaagag taattcagac aacttccgtc agtcatctat
    36961 tcaaggagtt atgaagcgaa ttaaagcaaa gggggcaact attgtaattt atgaaccaac
    37021 acttgaaaat aacactacat tttttggttc aaaagtaata aataatttag atgattttaa
    37081 aaatatcagt cacagtatta ttgctaatcg ttacgatagt aatcttgatg atgtaaaaga
    37141 aaaagtatat accagagatt tatttcaacg agattaaaaa ataacacctc agatttgtag
    37201 tgtctgaggt gtttctataa acttttataa gtaaaaaaat acaattatac ttcataaaaa
    37261 gacttatacc actgcgcaaa tttacgaagc ccgtcacgca aacttgtact tggtttaaat
    37321 tcaaaatctc gttctaacgc actagtatct gcataagtaa ctggcacatc tcctggttgc
    37381 attggaacta gttcggtatg agattcaaag tcgtaatctt ctggtagcac ccctgctgca
    37441 attagctctt gttgtagaat atctacaaaa tctagtaggt tttccggcgt attgttacca
    37501 atattataaa tatgatatgg tgggattggt agcccatctt ccccatcaat tttctctgga
    37561 gcgcactgca taaccctctt tactccttca acaatatcat ctatataggt aaagtcacgc
    37621 ttgcaattcc cataattgaa tatttggata ttatcaccag cacgaagttt atcagtaaaa
    37681 ccaaaataag ccatatctgg acgaccagca ggaccatata ctgtaaagaa acgtagccct
    37741 gtagacggaa tattgtaaag tttagaatat gcatgtgcca ttgattcatt acttttctta
    37801 gttgctgcat acaacgatac ttgattgtct actttatcct ctgtagaata aggaattttc
    37861 ttattagtac cataaacgga agacgatgag gcatacacta aatgttccac accagattct
    37921 ccattatcat atgaatgacg acaagcttca agaatattat agaatcctat tatgttagat
    37981 tcgatataca tatctggatt agtaattgaa taacggactc ctgcctgagc tgctaaattc
    38041 accacgatac ttggtttata attatcaaag aggaaatcaa ttgttgcttt atcagcaata
    38101 gaacctttga taaaattcca agtagatcct ggatggttct ttgcctccgt ttctatctct
    38161 gataatctat agttcttaat agagacatca tagtaatcat tcatattgtc aatgccaatg
    38221 atttttattg atgactgagt acgtaataac tcaagaacca aatttgatcc aataaaaccg
    38281 gctgcgccag ttattaatat tgttttattc tgtaaagatg tatttttttt cagcatctag
    38341 ttatactccc ttatttttat ttcatcatat gaattaatta aatgtcttca taatttgcaa
    38401 caagttatgt tcaaaggcat cataactata tagttctgct ctcttttttg ctgcttttgc
    38461 atacttattc ctcaaattat cgttttcaat caattcttct atagcattcg ctaaattttt
    38521 atcacattct tcatattggg gcgaataatt tctactgtct gtcattggac atactaatat
    38581 gcctgattca gcatattcta tattttcaca aatcgtgtct agcgttctat tcgataatat
    38641 ttctcttggc ccagatttgc aatctgcaca taagacagca gtttctgtag acattgcttc
    38701 aactaatgca ttaggaaaac cttcagttat agatggcatg acatataaat ctgcacgtga
    38761 catgtattta taaggatttg attggccacc tagcaaaaat attacgtttt gtagttga
//
LOCUS       DAZKSU010000019        38602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000019 DAZKSU010000000
VERSION     DAZKSU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.019"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG8Z3_001021"
     CDS             33..656
                     /locus_tag="ACG8Z3_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823356.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8Z3_001022"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG8Z3_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0823357.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG8Z3_001023"
     CDS             2152..3033
                     /locus_tag="ACG8Z3_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0823358.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG8Z3_001024"
     CDS             3036..3806
                     /locus_tag="ACG8Z3_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823359.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8Z3_001025"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG8Z3_001025"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0823360.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG8Z3_001026"
     CDS             5117..6280
                     /locus_tag="ACG8Z3_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0823361.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG8Z3_001027"
     CDS             6397..6807
                     /locus_tag="ACG8Z3_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0823362.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG8Z3_001028"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG8Z3_001028"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0823363.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVILYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG8Z3_001029"
     CDS             9380..9664
                     /locus_tag="ACG8Z3_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823364.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="ACG8Z3_001030"
     CDS             9824..10060
                     /locus_tag="ACG8Z3_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0823365.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="ACG8Z3_001031"
     CDS             10057..10455
                     /locus_tag="ACG8Z3_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0823366.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11122..12066)
                     /locus_tag="ACG8Z3_001032"
     CDS             complement(11122..12066)
                     /locus_tag="ACG8Z3_001032"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0823367.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12362..13306)
                     /locus_tag="ACG8Z3_001033"
     CDS             complement(12362..13306)
                     /locus_tag="ACG8Z3_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0823368.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13527..14741
                     /locus_tag="ACG8Z3_001034"
     CDS             13527..14741
                     /locus_tag="ACG8Z3_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0823369.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14763..16778
                     /locus_tag="ACG8Z3_001035"
     CDS             14763..16778
                     /locus_tag="ACG8Z3_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0823370.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16861..18234)
                     /locus_tag="ACG8Z3_001036"
     CDS             complement(16861..18234)
                     /locus_tag="ACG8Z3_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0823371.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18260..18544)
                     /locus_tag="ACG8Z3_001037"
     CDS             complement(18260..18544)
                     /locus_tag="ACG8Z3_001037"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0823372.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18546..20561)
                     /locus_tag="ACG8Z3_001038"
     CDS             complement(18546..20561)
                     /locus_tag="ACG8Z3_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0823373.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20740..21045)
                     /locus_tag="ACG8Z3_001039"
     CDS             complement(20740..21045)
                     /locus_tag="ACG8Z3_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0823374.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21134..22165)
                     /locus_tag="ACG8Z3_001040"
     CDS             complement(21134..22165)
                     /locus_tag="ACG8Z3_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0823375.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFPLAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22372..23247
                     /locus_tag="ACG8Z3_001041"
     CDS             22372..23247
                     /locus_tag="ACG8Z3_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0823376.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23399..24241)
                     /locus_tag="ACG8Z3_001042"
     CDS             complement(23399..24241)
                     /locus_tag="ACG8Z3_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0823377.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24333..26210)
                     /locus_tag="ACG8Z3_001043"
     CDS             complement(24333..26210)
                     /locus_tag="ACG8Z3_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0823378.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26207..27628)
                     /locus_tag="ACG8Z3_001044"
     CDS             complement(26207..27628)
                     /locus_tag="ACG8Z3_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0823379.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27631..31020)
                     /locus_tag="ACG8Z3_001045"
     CDS             complement(27631..31020)
                     /locus_tag="ACG8Z3_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0823380.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31277..32596
                     /locus_tag="ACG8Z3_001046"
     CDS             31277..32596
                     /locus_tag="ACG8Z3_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823381.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEEYVVQLLTQ
                     IYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSS
                     YIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITED
                     QLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(32669..33316)
                     /locus_tag="ACG8Z3_001047"
     CDS             complement(32669..33316)
                     /locus_tag="ACG8Z3_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0823382.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33324..34010)
                     /locus_tag="ACG8Z3_001048"
     CDS             complement(33324..34010)
                     /locus_tag="ACG8Z3_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823383.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34016..35089)
                     /locus_tag="ACG8Z3_001049"
     CDS             complement(34016..35089)
                     /locus_tag="ACG8Z3_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0823384.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35311..35985
                     /locus_tag="ACG8Z3_001050"
     CDS             35311..35985
                     /locus_tag="ACG8Z3_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0823385.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            35990..37330
                     /locus_tag="ACG8Z3_001051"
     CDS             35990..37330
                     /locus_tag="ACG8Z3_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0823386.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37479..38465)
                     /locus_tag="ACG8Z3_001052"
     CDS             complement(37479..38465)
                     /locus_tag="ACG8Z3_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0823387.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTYDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11883 a         7036 c         7304 g        12379 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcattcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
    11101 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
    11161 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
    11221 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
    11281 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
    11341 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
    11401 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
    11461 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
    11521 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
    11581 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
    11641 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
    11701 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
    11761 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
    11821 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
    11881 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
    11941 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
    12001 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
    12061 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
    12121 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
    12181 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
    12241 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
    12301 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
    12361 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
    12421 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
    12481 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
    12541 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
    12601 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
    12661 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
    12721 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
    12781 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
    12841 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
    12901 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
    12961 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
    13021 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
    13081 tttgtaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
    13141 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
    13201 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
    13261 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
    13321 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
    13381 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
    13441 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
    13501 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
    13561 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
    13621 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
    13681 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
    13741 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
    13801 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
    13861 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
    13921 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
    13981 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
    14041 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
    14101 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
    14161 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
    14221 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
    14281 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
    14341 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
    14401 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
    14461 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
    14521 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
    14581 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
    14641 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
    14701 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
    14761 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
    14821 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
    14881 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
    14941 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
    15001 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
    15061 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
    15121 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
    15181 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
    15241 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
    15301 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
    15361 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
    15421 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
    15481 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
    15541 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
    15601 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
    15661 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
    15721 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
    15781 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
    15841 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
    15901 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
    15961 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
    16021 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
    16081 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
    16141 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
    16201 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
    16261 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
    16321 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
    16381 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
    16441 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
    16501 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
    16561 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
    16621 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
    16681 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
    16741 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
    16801 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
    16861 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
    16921 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
    16981 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
    17041 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
    17101 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
    17161 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
    17221 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
    17281 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
    17341 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
    17401 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
    17461 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
    17521 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
    17581 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
    17641 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
    17701 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
    17761 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
    17821 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
    17881 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
    17941 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
    18001 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
    18061 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
    18121 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
    18181 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
    18241 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
    18301 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
    18361 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
    18421 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
    18481 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
    18541 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
    18601 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
    18661 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
    18721 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
    18781 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
    18841 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
    18901 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
    18961 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
    19021 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
    19081 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
    19141 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
    19201 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
    19261 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
    19321 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
    19381 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
    19441 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
    19501 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
    19561 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
    19621 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
    19681 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
    19741 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
    19801 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
    19861 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
    19921 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
    19981 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
    20041 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
    20101 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
    20161 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
    20221 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
    20281 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
    20341 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
    20401 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
    20461 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
    20521 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
    20581 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
    20641 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
    20701 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
    20761 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
    20821 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
    20881 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    20941 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
    21001 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
    21061 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    21121 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    21181 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    21241 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    21301 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    21361 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    21421 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    21481 tgatgcggaa ataactagat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    21541 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    21601 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    21661 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    21721 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    21781 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    21841 ttgctaatgg aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    21901 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    21961 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    22021 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    22081 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    22141 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    22201 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    22261 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    22321 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    22381 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    22441 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    22501 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    22561 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    22621 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    22681 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    22741 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    22801 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    22861 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    22921 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    22981 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    23041 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    23101 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    23161 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    23221 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    23281 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    23341 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    23401 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    23461 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    23521 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    23581 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    23641 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    23701 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    23761 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    23821 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    23881 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    23941 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    24001 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    24061 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    24121 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    24181 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    24241 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    24301 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    24361 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    24421 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    24481 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    24541 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    24601 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    24661 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    24721 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    24781 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    24841 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    24901 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    24961 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    25021 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    25081 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    25141 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    25201 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    25261 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    25321 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    25381 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    25441 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    25501 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    25561 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    25621 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    25681 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    25741 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    25801 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    25861 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    25921 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    25981 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    26041 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    26101 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    26161 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    26221 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    26281 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    26341 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    26401 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    26461 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    26521 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    26581 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    26641 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    26701 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    26761 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    26821 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    26881 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    26941 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    27001 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    27061 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    27121 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    27181 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    27241 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    27301 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    27361 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    27421 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    27481 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    27541 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    27601 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    27661 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    27721 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    27781 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    27841 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    27901 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    27961 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    28021 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    28081 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    28141 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    28201 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    28261 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    28321 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    28381 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    28441 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    28501 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    28561 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    28621 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    28681 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    28741 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    28801 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    28861 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    28921 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    28981 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    29041 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    29101 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    29161 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    29221 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    29281 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    29341 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    29401 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    29461 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    29521 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    29581 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    29641 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    29701 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    29761 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    29821 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    29881 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    29941 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    30001 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    30061 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    30121 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    30181 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    30241 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    30301 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    30361 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    30421 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    30481 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    30541 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    30601 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    30661 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    30721 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    30781 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    30841 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    30901 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    30961 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    31021 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    31081 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    31141 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    31201 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    31261 atgtaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    31321 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    31381 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    31441 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    31501 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    31561 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    31621 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    31681 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    31741 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    31801 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    31861 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    31921 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    31981 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    32041 gattttttca gaattttcgg agaaagaaaa aaacgaagaa tatgttgttc aattattaac
    32101 gcaaatttat tttttctccc gtattttccc atacgatacg actcatttga cgatccgtgc
    32161 aacttacttt gcacagcaga tgcaacaccg ccagatacgt ctatatgctg gatttcgtgc
    32221 agaacttcaa ttttattcca ctctactgaa tcagaatctg atttattatt tatctagcta
    32281 tatcttttgg atgcttatcg gtaatcctga aattcaccat tatacttctg ataaaaaaat
    32341 actgattatc agcgacttaa gtcttcgaca tagtcaatat attgaagaat atatcagtga
    32401 tattcttgct gtgcataaaa ttcactctga gacaactgct attactgaag atcagctgtc
    32461 gaaatacaat cttctcgagt atgatctgat cgttacgaat caacccattt tgaatgccca
    32521 tgttcctcat atattgattg atgatagtgt gtcattcgca aatgaagaag aactcatccg
    32581 cttattcgag ttatgaataa tcaaaaaaca gctatgtata aaccaccctg ataaaaattt
    32641 caggacaaat acacatagct gtttcttttt attgtattcc ttcagcaatc caacgagatt
    32701 tatagcgaaa gtcttttaac aactcttctt ttgtccaatt atttgcaatg agatccgtca
    32761 aagtgttcat tacgagggtc atggaaaagt ataggaaact aactgcttct tccgacgatt
    32821 gaatggagaa atgattgatt cttaataatt ccagccattt ttcgttcatt ggagaatcag
    32881 ccgatagttc tctacgtttg gttgtctgct gatcatttcg ctgggttaac aagtataacc
    32941 tttgccaata gatactttcg tgatctaagt cgcagcacca ctctaaatat ttttcgatcc
    33001 ccaaaaagaa atgttgtttg ttttcgctaa tgaattttaa gatatcttga tggactatgt
    33061 gagaactgct tttgatcgta tacaaataag catcttccaa atcttgaaaa tatttataaa
    33121 aagcaccgcg cgacatcccc atcttgtcaa cgatttctga gactttgact tggctgatgt
    33181 gctgtgagca aaatatctcc agtaataggt catcgattct ctgtttcttt gcgttcgata
    33241 aattgaaaaa agtttctttt ggcatccaca tctctccttt atctctgatt atacgtggat
    33301 agcccttcat tgtcatgaag gattcattcc ttctttttct cttcttctgt caagtaccca
    33361 tcttccattc gataaacgcg atcactccat tgaatcatac ggacatcatg agtaaccata
    33421 atagtcgctt tttgtttctc atgagcttct ttaactagta atttgacgac ttcatatgca
    33481 tgatctgtat ccaaactagc tgttggttcg tctgctaaga tcaagcttgg ctcattatat
    33541 aaagcgcgag caattgccac tctctgtctt tctccaccag aaaggtcttt aggataacta
    33601 tttttaagtg actggatatc taaagattgc agcaaggaat cgatttttgt tgaatcttct
    33661 ttttttgaag taacttggtc aactagttca aattgttcct ttaccgttaa aaaaggaatc
    33721 aaattagagc tttggagaat aaatcctatt tctttaaagc gtaaatctgc tcgttttttt
    33781 tctggaagct cagttagtga gtgaccgttg atcactattt ctccggaagt cggtgtttgc
    33841 aatcctcctg aaatggtaag gaaagtactt ttacctgaac cagaaggacc aatgatgctg
    33901 acgaattctc cttcctttac ataaaaatca attgctttca aagctgagac ctttgtatga
    33961 ccagaaccaa atgttttggt aacttttttc atcgttaata tttttttcat ttttattaac
    34021 ctcctattgc tgtaattggg tctactttgg ttatcgtacg tatcgaaaac aatccgccta
    34081 gcattgcgac aatgatcaaa atcccactat atagcaacca ttgtgaccaa aaaacagcaa
    34141 aaggcatcgc gcttggtaaa atcagactag tcaaataagc aaacaaaaga gcaagcagaa
    34201 cgcctaccac cccaacaata aaggactgtg ctactaaaga tttagcgatg aagaaatttt
    34261 tgatcccttg tgctttcatt actccaaaaa tagcagtctt ctgaagtgtc atcacgtaca
    34321 tgaaaatacc gaccacggca gcaaccacta agaacaaaaa gtatatcatc gcatttaagg
    34381 tcatattttg ggcagaatag ccaggaatac tttcaatgaa tgtcccaata gttaataact
    34441 gtagaccctt cgatgctttt tctttcgaaa ttttagctga atcctttatt acgatcccat
    34501 tgatcggctg ttcttcttca gaagcaaacg gctgttgtcc atacttcaaa gcagtccacg
    34561 tatctaggga cgcatagatg acgggagaaa ctgtgtaata agtttcagga aagataccga
    34621 caatcgtcaa aggatcgtcg taactgccaa ttttgatcgt atctccaatt ttatagcctg
    34681 aatcagccag attttgagag atgatgattt cattctttgt tttaaattca tgccctttcg
    34741 tgagttttgg taataaaaat gcttctttcg ttgtgccgaa aactgtaaca ttttctttgt
    34801 tttttccttt gattgctccg ctgtacaatc cgattggtgc tttctctcct ccttcaatgt
    34861 atttcagatc tcctcttgtt aattgtgaag ctgcaaatac ttgatttgca tcctccgaca
    34921 aaacaatctc ttgtgcgtcc caatcatcga ttgctttttt aaactcttct gaaagtccat
    34981 tcgctagtcc tgacaacatg aatacgacat acgcaatcaa gaacataata ccgacgatca
    35041 agccatatcg taatttagaa tagcgaattt ctttgatagc tagaaacatt tgtttttcct
    35101 ccctatgata gattgatagt gacaaaatgt cacttgttga atagtgtaac tctaaagtga
    35161 caatttgtca cttgtgttgt ttttttctta tcaataaatt gtctaagaca tctttcgata
    35221 aaaccttaac atgagtcata aaaaatttat ttaagcctta tttatgcttt ccatgataaa
    35281 ctaaagtgac caaatcaatg aggtgggaaa atgattaaac tattagtagt agaagacgat
    35341 aaagtattat ccaataatat aaaagaaata ctcgctgcaa tcggcgagat aacacaagtt
    35401 tatgatggta gtgaaggact gttcgagttg gaaagtggag tttatgatct tgcaatcttg
    35461 gatttgatga tgccggaaat gaatggatat gaagtgttag caaaaatacg gaaagaaaaa
    35521 atacagactc cagtattgat tttgactgca aaagatagtt tggaagataa gattgaagga
    35581 tttgaacgag gtgcagatga ttatctaaca aaacctttct atcgagaaga actgatcatg
    35641 cgcgtgaaag cattattgaa acgctcgcta ggtctctttg atgaaaatgt tctgaagtat
    35701 aaggatatca gttgtgattt aaaaagcaat gaagtcttgt ataaagaaca agttttgccc
    35761 atacaaggaa aagagtttga attgctcgtt tattttttac aaaataaggg aatcattttg
    35821 acaaaagagc agatatttga ccggatctgg ggatttgatt ctgatacgac gattacagtt
    35881 attgaagttt atatgagcaa cttacgaaaa catctgcgaa gtactggatt ggataaagaa
    35941 atcaaaacct tacgtaatgt cggttatata ctgcaaaggg tgtgatgcaa tgaatggaga
    36001 tattacacga aagcaacgta gtcgcttttt tgcgacaaat gtagcagcat ttgcttttat
    36061 cttttttctt ttgggattca ttactttaca gatattaaac caatctgcat acagagaaac
    36121 agatctgtca ttagagaaaa taaattttga ttctcgaaca gtacaattag agatacaaag
    36181 atatcagcaa ggcgatccct ttttaaaccc gactcaaacg gaacaaaaca gaacaaacat
    36241 ggaacagcca ttaatggaac catcaaatga ccgttttaac actcaagtga tcttgtggtc
    36301 taaaaatggt gagatattga ataaagcagc tattggtggg cgtttcaacc aaatcaaaga
    36361 attaaaaatg gatacgaaaa atctgaatac catccaaaat attgaattaa atgaagacag
    36421 tggctcatct gccctctctt tccgttcgat tacgaaaaaa gcggagacga atcaagatgg
    36481 cgttgcctac atccaaattt tagaaaatac aaatcaaatc aagaattcat tagcgacttt
    36541 tcgcacgatt atgattttgt gtatgattgt tttttggatt ctctctattg gtatcagtta
    36601 ttacttgtct agtctaagca tgcgccctat tattctttct tggagaagac aaaaagaatt
    36661 tgttgagaat gcttctcatg aattgcgcac accgttaacg attattcaaa atagtttgga
    36721 acatcttttt accaagccta atcatactat tattgaagaa tccgaaagca tcgctcaagc
    36781 attgtcagag acgaggcgcc tcactggatt gacttcggat ttgctgacaa tcgctcggaa
    36841 tgattctgat cagcagctgc tttcaaagca aatgatcaat acacaagaat atatcgaaca
    36901 acttgttaaa ccatttcaag agatggccat catagatgga aaagagttta ttctccataa
    36961 cgatgcaaca ttagaagtac caattgatga aaaaaaaatc catcaagttt tggttatttt
    37021 gctggataat gcattaaaat atacgaatcc agaagataag atcattttgg attcatctgt
    37081 taaccataaa gaatggctga ttcacgtaaa gaatactggc ccttctatca gtgaagaaga
    37141 tcaagcaagg atttttgatc gattttatcg ggaagatcaa tctcgggcga aagagactgg
    37201 cggatacggg cttggcttgg caattgcaaa acaggtaatc gaacagcatc atggacagat
    37261 ttctgtatcc aattatcttc cacaaggagt cgattttaaa gtccaattgc cgttgaagcc
    37321 taaaaaataa tgcttatccc taaaaaaggg ctgtgacaaa aatcttgtca cagccctttt
    37381 ttgttgcgaa taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcttt
    37441 aagctgacca cttctgtcac aacctcttta gcttacgctt acgatttttt attgcggtct
    37501 tcaatgtgta catcaccgct tgtgtcaata tccaattctt ctcggctaac atcttctgtc
    37561 actttttcaa catcttcatg ttcttcttta tgaatcccaa cttcttcacg aataactgta
    37621 tgtttgctta catctacttg ctcttctttg attgggatag taatcgtgtc atcttccaat
    37681 ttttcgtctg ttccctgtga agattgatct gttactggtt tgcgttcgat gacgatttct
    37741 tcacgtttga ccggtacttc taccgtttca gtatcgttga ccacatgttt gtggatgtcg
    37801 acttctccag ttgtcacatc atatgtattt acatctaatt tttcttcttt tagttgaatt
    37861 gtatcatcat ctagatttgg tttgcgatcg atgtcttcag tcttgcttgt acccattgta
    37921 tcagtctcgc caggtacgtc catcggatca gttcctggtg tcatattcaa tggatccgtt
    37981 gtattttgaa agcctgtcgt gccacctacg tccattggat ctgttccagt gccagtttct
    38041 aatccagtcc ccattgtgct tgctgggcta acagtatctt gtgtatccat tgtatcttct
    38101 gttgtttctg gctgacgata gccttttact gtgatgacaa attttccatc agaaatatct
    38161 cctctatatt gatataaagg atcattttct ggtgtagccg tctcagaatc ataggtatca
    38221 aaagaaaaag tatcttttat tttttcccat aaagaacggt cttcttcacc atccatccga
    38281 ttttcatctg tttcaacatc tataccagac aatccgtcaa atcctagtct agatgctgct
    38341 tcatcagtag tataaataac gatatcatct cgttcatacc cttcgttacg caaccgttca
    38401 attactgata atgcttcttc cctcgtacta tagcttccga tgaccgttaa atttttctct
    38461 tccatgtaaa ttcctcctag caaatagatt tatgattggt atattgttac ttataaattt
    38521 attctatagg agacgattgt tttctacaaa tatttagatt ggaatttgat taaaacaatt
    38581 taattgaaat ttgaaaacat tt
//
LOCUS       DAZKSU010000020        38078 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000020 DAZKSU010000000
VERSION     DAZKSU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.020"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..767
                     /locus_tag="ACG8Z3_001053"
     CDS             66..767
                     /locus_tag="ACG8Z3_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0823388.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ACG8Z3_001054"
     CDS             764..1885
                     /locus_tag="ACG8Z3_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0823389.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ACG8Z3_001055"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ACG8Z3_001055"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0823390.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ACG8Z3_001056"
     CDS             3264..4172
                     /locus_tag="ACG8Z3_001056"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0823391.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ACG8Z3_001057"
     CDS             4207..4479
                     /locus_tag="ACG8Z3_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0823392.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ACG8Z3_001058"
     CDS             4490..5536
                     /locus_tag="ACG8Z3_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0823393.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="ACG8Z3_001059"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="ACG8Z3_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0823394.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="ACG8Z3_001060"
     CDS             complement(8547..9233)
                     /locus_tag="ACG8Z3_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823395.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="ACG8Z3_001061"
     CDS             complement(9211..9705)
                     /locus_tag="ACG8Z3_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0823396.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="ACG8Z3_001062"
     CDS             complement(9725..10945)
                     /locus_tag="ACG8Z3_001062"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0823397.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="ACG8Z3_001063"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="ACG8Z3_001063"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0823398.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            13123..14034
                     /locus_tag="ACG8Z3_001064"
     CDS             13123..14034
                     /locus_tag="ACG8Z3_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823399.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            15045..15281
                     /locus_tag="ACG8Z3_001065"
     CDS             15045..15281
                     /locus_tag="ACG8Z3_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823400.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            15394..15636
                     /locus_tag="ACG8Z3_001066"
                     /pseudo
     CDS             15394..15636
                     /locus_tag="ACG8Z3_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15726..15965
                     /locus_tag="ACG8Z3_001067"
     CDS             15726..15965
                     /locus_tag="ACG8Z3_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823401.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            16112..20395
                     /locus_tag="ACG8Z3_001068"
     CDS             16112..20395
                     /locus_tag="ACG8Z3_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0823402.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKAIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            20494..20859
                     /locus_tag="ACG8Z3_001069"
     CDS             20494..20859
                     /locus_tag="ACG8Z3_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823403.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            20861..21313
                     /locus_tag="ACG8Z3_001070"
     CDS             20861..21313
                     /locus_tag="ACG8Z3_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823404.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            21762..23237
                     /locus_tag="ACG8Z3_001071"
     CDS             21762..23237
                     /locus_tag="ACG8Z3_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0823405.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            23260..23571
                     /locus_tag="ACG8Z3_001072"
     CDS             23260..23571
                     /locus_tag="ACG8Z3_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823406.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            23932..25188
                     /locus_tag="ACG8Z3_001073"
     CDS             23932..25188
                     /locus_tag="ACG8Z3_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0823407.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            25185..25730
                     /locus_tag="ACG8Z3_001074"
     CDS             25185..25730
                     /locus_tag="ACG8Z3_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823408.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            25731..26231
                     /locus_tag="ACG8Z3_001075"
     CDS             25731..26231
                     /locus_tag="ACG8Z3_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823409.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            26590..26868
                     /locus_tag="ACG8Z3_001076"
     CDS             26590..26868
                     /locus_tag="ACG8Z3_001076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823410.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            26888..27232
                     /locus_tag="ACG8Z3_001077"
     CDS             26888..27232
                     /locus_tag="ACG8Z3_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823411.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            27251..28162
                     /locus_tag="ACG8Z3_001078"
     CDS             27251..28162
                     /locus_tag="ACG8Z3_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0823412.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            28241..29233
                     /locus_tag="ACG8Z3_001079"
     CDS             28241..29233
                     /locus_tag="ACG8Z3_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0823413.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            29230..29448
                     /locus_tag="ACG8Z3_001080"
     CDS             29230..29448
                     /locus_tag="ACG8Z3_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0823414.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            29461..29871
                     /locus_tag="ACG8Z3_001081"
     CDS             29461..29871
                     /locus_tag="ACG8Z3_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0823415.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            29923..32451
                     /locus_tag="ACG8Z3_001082"
     CDS             29923..32451
                     /locus_tag="ACG8Z3_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0823416.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            32467..34677
                     /locus_tag="ACG8Z3_001083"
     CDS             32467..34677
                     /locus_tag="ACG8Z3_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0823417.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            34685..35776
                     /locus_tag="ACG8Z3_001084"
     CDS             34685..35776
                     /locus_tag="ACG8Z3_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0823418.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            35797..36339
                     /locus_tag="ACG8Z3_001085"
     CDS             35797..36339
                     /locus_tag="ACG8Z3_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0823419.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            36534..36791
                     /locus_tag="ACG8Z3_001086"
     CDS             36534..36791
                     /locus_tag="ACG8Z3_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823420.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            36805..37137
                     /locus_tag="ACG8Z3_001087"
     CDS             36805..37137
                     /locus_tag="ACG8Z3_001087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823421.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            37182..37730
                     /locus_tag="ACG8Z3_001088"
     CDS             37182..37730
                     /locus_tag="ACG8Z3_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0823422.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            37737..>38078
                     /locus_tag="ACG8Z3_001089"
     CDS             37737..>38078
                     /locus_tag="ACG8Z3_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823423.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKR"
BASE COUNT        12931 a         5988 c         8105 g        11054 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatcct ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggatgagc aagatagtgt gaaataactt ataatcaatc gaaaaaccta ttaaataaac
    12961 ctttttgagt agatgcaaca ctgaggatta tctgtataaa agattttaaa ctgtcatttg
    13021 gttatcacgg cgatactaaa attaacaaaa ttggaatatt tttcttgaag atttttagct
    13081 gtcagtatat gatgaaagca tctcatagga ggtgcttttt tcatggttga taattcaagt
    13141 ggaagggttc ttaaatctct aagaaaagag aaaaaactat ctcaaaaaaa gttagctgat
    13201 ttagcaggta tttcacaaag tactttagtt aaatatgaaa aagggtcaag aaaaatccct
    13261 aaagacgtag ataatactct aagcaagatt ctgaatatag aaactctaat aaaagatgag
    13321 gaagataaga ttgagattct catcggtaaa ctaattgcat acagagatat gaataagtta
    13381 cttaacaaag agttagctga taatatagga attagtgaag tattactcag ttatgtgtta
    13441 aatagaaaga gaaatccgtc aaaagaaatg caaaaaaaaa tcgatatttt tctattaagt
    13501 aatgaaaaag aaattttgaa ggagattaat cgtgatagtg agatatttag tttatcaaaa
    13561 gacgataaaa ttgttatggg aaaaagaata cgagaagtcc gaaagaaccg ggaagagacg
    13621 cttgaaaaat ttggtaaaaa ctttaccatc tatacaggca aaaatgttat aagtagatgg
    13681 gaaaaaggta ttaatattcc agatattgaa aaactaatga atattgccta tcttggtaag
    13741 gtcactgttc cttaccttat gtacggagaa gattataaga atatacttcc aaaagatgaa
    13801 agagttagcg attttaaaaa gataaattct tttagtatgg gattaaggat gaggaaaatt
    13861 aggaaagatt attatctaga aagggaggag tttggaaagc ttttttctcc ttcaatttca
    13921 aaatggtcca tagataggta tgagaatggt agagatatac ccaatactaa tcgaattatc
    13981 caatatgctt atataggaaa tctttcttta gaatttttga tttacgggat ataaaaatag
    14041 tttacgtcga tacttattaa gttatcgacg ttttttatta tagaaaaaat ttataaaagg
    14101 aggtttcttg atatgataag aaacaaaaag ggtttgttga aagttgcttt actggcagct
    14161 tttttctaag taactttttg acaagctcaa tggcaacaca agcggaagaa gtaatacaag
    14221 aactaactgt tatgaataac agctagataa aatgggctat gtatgttatc caacaattta
    14281 gtaatcacta gaagcctagc gaaagttcgc ttgataagtt atagatgagt attgatttgt
    14341 agtgtttcca aatcaaaagg gtacagagcc acatgaaata tgttgtcctc tgcagcgtca
    14401 ggatgggaat ccataccatg gagattcgga gatctgtgag ggaatactta cgtggttaat
    14461 atgtcagttg tgttggacgt agtaatggtc atagtgggta gattgactga attcttatat
    14521 gagaaaatga aaccaacaag aagatagggt tatttataca gaattgggga tcttttctgg
    14581 aaaagtaatc ggataatgcg agattcgtac ggtctttttt aaatttctct aaaaaaaacg
    14641 gcatcgaatg ggttgggtga ttgataagca atgaatcata gactgatata gaataaaaaa
    14701 tgattctatt tatcttttta catctatcgt tttttcaagt agccgcaaat tatatcagca
    14761 ttcaacaatt tttgtataca agttctagta aaaggatctg aagaaaaagt tggttgttgt
    14821 gaagataaaa aaagaatcag gttttactga gtttttcttc aaaatttgtg ttattaggtt
    14881 aggacaaaga taagaagaag tatgaagatt taaaaaagga atgaggaaaa gatagtaatt
    14941 tccgattatt ggagtttttt tatttataaa gaagaagcga ataagttgtg attcctagat
    15001 ttcgcgcgtg attaattaat gagcaaaaaa tgcggaaagt tttagtgttt aatacacgag
    15061 gacaaagttt cttaggtatt gaaggcacac gtgtaacggt tgatgggtgg tgtgattgtt
    15121 tcgaggttgg aaaacatgtt gaacttatgg catttactga gctaaaagat aagaacaacg
    15181 tggaattttt cgagagtgat attggttggg acgatcatga agaagtttat ggaaaattta
    15241 ttttgcggaa gggaaattct tatacgaatg gggaagcata agtagcgatt tgttagagat
    15301 cattgatagg attgaaattg tcgggaatat ttatgagaat ctagagttat ttgaggtgat
    15361 agaatgagtg atggttttga acaagtatca ataattaaaa atcaagttcg ggagattctt
    15421 agaaaaaaag gctatttggt agatagttat tttgaaggag attacgaaac atgggtaggt
    15481 gtctatgctc gtccagagaa taaacctacc tatttagatc ctacgacgtc agaagatgcc
    15541 tatttacaaa ataggtatcg agtagatggt tttaagcaag actttgcgga gtggtttgaa
    15601 tgggaaattg aaaacggaga agtaaaagaa gaataagagt acttgttgta tcaaaataag
    15661 ccagtagaac aaccaaacca gtagctagta atgacaataa tactgaggaa tcggatcatt
    15721 atggtatgat tgatatttct cttccagaag atcttaagcc agatcctgta acaattggca
    15781 atagtgtcat gatttttgat tcattagatg aagctatgtc atatggttgg aaagaaatta
    15841 aggatgaagg tagtaattgg tatggcatga gatttggtga tggtgaagtt gccaaaaaag
    15901 ctggtattcc aattgggaaa tggacagttg atttccacga agcaggttct ttttttgttc
    15961 tgtaattata gtgggagggt aaaatcattg atgaaatgtg ttcagataag agcgccagat
    16021 aaaaatagaa gagacagttc acgtcgttat cacttttggt aacggcgttt tgttttatta
    16081 tataaaattc tcaggaaaag gaggaattct tgtgtatgca aaaacgaaaa tgaattggtt
    16141 gaaagcactc gcattggtgg ctttttctca attctgtttc ttggtgggaa ctgctcaagc
    16201 acatgcagaa gaaacgacgg ctatacaacc tacgatggaa gccattgccc aagtcgcttc
    16261 tgaagtgaca gaagtccaac cagcagaagc cggggtccca gctggtgaag ccactgttga
    16321 acaggcccct gtgacagaag cgccaacggt caattccaaa acgactctcg atcagacgat
    16381 tggggaaaca caacacccag ggaatagtcc tggtacttca caaccagttg aagaaactgc
    16441 ctcaattaca acccctgctt cagaaacgtc cactctagaa ggagaaaaag ctgccgaagc
    16501 aacgccttct gtgcagccag agtcttctga gaaacaagcg gaagaaaaga aacgaataga
    16561 agaaacagtg gaacaaccag cggctactgc tgacaaagaa ggaaaaaatg aggaaaacgt
    16621 tgaaaaaaca atctcttcaa ccgcagctag tcaaaatcaa ccaacgaaac caaacaaaga
    16681 atctacggct agtattgaaa atcaaaagaa aaaagagcag aacattccaa aaacaggtac
    16741 acctacactg ttagtaacag taccaaccaa tcgttcggtt cgtgttcgcc gtgcaaccaa
    16801 tgcagctgtt ttaggtggtc ggaaaattga ttggggcgaa aatattggtg ttacttctag
    16861 tccgaacaat ctaaacttca ctttaaatta tatggttcgt tttgagatta atggacaagt
    16921 ggcgttttgt attgagcctg gtgtctcagc cgcagaaggt caagtttata cagctactgc
    16981 tttgaaaaac tatttgaaaa attcaaatgt acggaaaaaa acaagcttga tctcttattt
    17041 aggatatgtc acaaataaag acaaatccga tgaacaatac attgcggcac aatttatgat
    17101 ttgggaagct ttgggcacaa aaatcccaat caaaaatatc cgtgttgatt atgatcgtcg
    17161 aaaagcagaa attcaacgtt tgattaataa ctttggtgaa aaagcaactt ttaataatca
    17221 aacacatacg gtaaaaattg gcgaaacgtt gaaagtaaag aataaaaatg attttttgaa
    17281 atatgtgaaa gacattcaaa aaccaaaagg tgtcaatgta tctattcacg gcaatgaagt
    17341 atggattaca gtcacaaaag aggcaccgga taaagcaaaa attcaattga ataaaattaa
    17401 agttcaaggt gcgccattag cctatcgtcg tccaggagct caaacaattg gtgtgttaca
    17461 tccaacagaa cctaatgcgt ctgttattaa tttacaaatt ctaaaacaag gaaacattcg
    17521 tgtgtttaaa gaagatgcgg aaactggtgt gaaagcacaa ggtgcagctt ctttagaaaa
    17581 tgcggtctat ggtttgtatc aagcagatgg gaaaaagatt aaagaaatta ccttgaaaaa
    17641 aattaacggc aaagtacagg cagaaattaa agatcttgat cctggcaaat atgttttaaa
    17701 agaaatcaaa gcgccaaaag gttatgtgct ttctgatgaa gcaattgaaa ttgttgttga
    17761 acctggaaaa actgtcaatg cgaccgcaaa agatcaagtg atcaaaggaa ccattgattt
    17821 agtgaaagtt gctaataaag atttagtcga ttcaacgaat ccagataaca aaccaaaatt
    17881 agctggcatt gaaatcactt tgacgtcaaa aacaactggc aaagtggtga aaaccgttgt
    17941 gacagacaat gatggttatg ctacttttgg taaaaatacc gttgtctttg atacgtacat
    18001 tctttctgaa accaaaggaa aagaagggta taaactattt gaaccacttg aagtcacaat
    18061 ttctgaaaat gggcaaactt tccattatgt tttagaagat aaagcaattg aacaacgtct
    18121 taaagtggtg aaagtggatc aagaaacagg aaaagtgatt ccacttgcta atacagaatt
    18181 caagattttt gatactttag ccaatcgtta tgtcacaatg gagattccaa atgatacaga
    18241 aacaacggat atctttaaaa ccaatgaaaa aggctttttc ctaactaacg gtacacttac
    18301 ttatggaaaa gatcgctatc gtttggaaga aatcaaagcc cccgtaaatt acgttttgaa
    18361 tcaaaaccca ttagtgttct cgattaccaa tgatacagaa ccgatcaaag tgatccattt
    18421 tgcgaaccgt caagcacgga aaagtgtaaa attattcaag tatgaagaat ggaacaagaa
    18481 gaaaacgccg ctcgcaggtg tgaactttac cttgtataac ggtaaaaatc aagcaattgg
    18541 tgaatatacc actgatcaaa acggggaaat tttagtgaaa ggtctagttg ccgatgacta
    18601 ttacttcttg gaaaatgccc cattagaaag tttccaacca aatacagata aacaagcatt
    18661 cacggttgca ttcaaacaaa atgcgccaga tgatggggaa acgttagtgg tttctgtacg
    18721 taattacttg attccaccaa aactagaaac acaagcgtca aataaagccg atggtgaaaa
    18781 agtggttgat ccattagaaa cgatcgtcat taaagacagt gtttcttata ccaacttgtt
    18841 tgtcggaaaa gaatacacca tttcaggagt attgatgaac aaggcaacga atgcgccaat
    18901 tttacaagat ggcaaagaaa tcacaagtac cttggccttt attgcagaca aagtcaatgg
    18961 cacaaaagac cttgaatttg tggttaatgc tagtgttctg aaaggtcaaa cgatcgttgt
    19021 ttttgaaaaa cttttccgtg atggcattga agttgcagca catgcggata tcaacgatga
    19081 agcccaaagt gtgaaagtga ataatccaac cgttcatacc acggctgtct ttgaagacgg
    19141 attgacagtc gcagacccaa caggattgtt taccttaaac gataccgttg aaattaccga
    19201 gattcttcca ggaaaagcat acttggtcaa agggatctta atggataaag caaccggtga
    19261 acccttgctt gtgcatggaa atacggtaga aacgagtgtt ggctttattg ccaaagcaga
    19321 aaatgaaacg gtgatcgtac catttacctt tgatttgaca ggtataaatg gtcgtgaaat
    19381 cgtcgtgttt gaaagtcttt ttagaaatga tgaagaacta gcaaaccata aagacatcaa
    19441 cgacaaaggg caaacggtac gagtaactaa cccaagtgta cgtacaacgg ctacacataa
    19501 acccactggc ttgaaagagg ttaatccatt aacgaaagtc accattacag acgctgtgaa
    19561 ataccacgat ttggtcgttg gaaaaacata cacagtcaac ggtgttctaa tggacaaagt
    19621 aacggaaaaa ccagtgctag tagatggaaa agaagtcaca agtaccttaa cgtttgtggc
    19681 agaaacaacg gatggtacag tcaatctaga ctttattcta gacgcacgtg cgttgcgagg
    19741 caaagaagtc gtggtctttg aagatctgtt tagagaaggc gtattattgg cttctcacgc
    19801 agatattacc gacaatgacc aaaccatcaa aatcagtaat ccaaccatta cgacgaaagc
    19861 gacgaataaa aatggcggaa aagagatctt ggccttgcca aatcaaactg tgcttgaatg
    19921 ggtcaaagta gacggtcttg cgattggtca aatctatcgc ttaattgttc aaggctactt
    19981 aacaccagac ggcacaccat ttgaaggcac tcatgcggaa aaagtctttg cggccgataa
    20041 agaaatgatg gaattcttat ttgaattctt agtggacggt cgcaatcttg caggaaaagg
    20101 cttgagtttt gcggaatggc tccaagcgaa aacggaagat gagaaagaaa cgtttgaaga
    20161 agtggcgaaa cacaatgtag atctaacaaa caaagatcaa acggttcaaa ttgtggaagc
    20221 gccaaaacca ccgcaaccag tgaaaccaga acaacgggtt aaaccagcta ctgttgtacc
    20281 aatgttgcca aaaacaggtg aacaaacaag tagtatactt atggttcttg gattagtagt
    20341 attagtcatt gctttaggta taacacttta taaagtacgt aaagccaaag attagtcatg
    20401 aggaacaagc ggttaggatt tttaaccgct tgtttgattg taatgtagaa atatttagaa
    20461 tttattttgg tttttaagag gaggaaaaac tttatggcag aaaaagcatt gcgtatgaag
    20521 aaagagtttt taggtcaagt atttttattg ggttcacggg aacctaatcc accaaaggaa
    20581 attaatggtg tgtctgggcg atatgttcaa ttacaagcag agtgttcgcg agaagaacag
    20641 cctgcttttt ttgtttatct cacagctgaa gaggataaaa gattacgatt accagaaaaa
    20701 gaggtgcgta tttcatttga agaagcttat tttgtttttc gtaaagaacg agtgaatagt
    20761 cgtgaaacaa atgatatggg acaaacagag cgtcgaacaa ctggctatca agatgttggt
    20821 gtctttttaa ctggtgtaaa aattggggag gttttgtaag atgggattaa aatttccaaa
    20881 taattatgac attacagaag cattagatga aacttgttta ggagccgtac gctttggcgc
    20941 attagaagag tctgatcgtg tatttgataa tgaaacaggc gattatacgg gagaaattcg
    21001 atcacagcga gcacaattag tttttggaaa tccagatgaa ccaaagattg cacgacgagt
    21061 taaatttcca gcagatgtag atttgactgg ttttaaacga cgtgatcctg tgaaattaat
    21121 tgatccagaa tatcatgtgg tatatattcc agcacaagat ggcaatcgtg aatattcaga
    21181 tttagtgatt tatgcgaaag gattagagaa agtagaagct ccaacaaata atcctaatat
    21241 gaaacaatca caagaaaaag gtttagatca aaaacaacca agtgttgatt ctaaggaaaa
    21301 agagaaaaaa taaggttttt ttgaaagaat aggaaggagt cttcctattc tttttatcta
    21361 agatagaagg aaggggagat tttgaattca gataaaacca atcaaatact ttgagagatt
    21421 gtgcttgtta tcaaaatatc caacaattga ttgagtattt tggagacaat ccaacgattt
    21481 ttgtacaaac aaatgcatat atttgtgcgg ttattgggca gtgtatgttt ttacgtgatc
    21541 ttgttcgttt ttcattaccg taccagtgga aacaagatca tcaggaagtg atttggattc
    21601 gtatagtacg aatatgtaat atgtgtgcac acccttataa aaatcgatta aatctttttc
    21661 gattatgttt ttttactaaa tgtgatattc ctgtattagt tgctttttgt caaaagtttt
    21721 tagcacagga aaagtaaaaa cagaaaggag gaatgtgaca attgaaagct cattttttca
    21781 agtatcgtgg tcggcgaatt cgctatttca atcgttattt atggttttgc tattatggtt
    21841 tctttttgtt tccatttttg attctgttag gttggtttag ttggaaagta ttttatccac
    21901 gtttaagtac gttttcgaat tggcaaatgt atcttattcc tggaataagt attgtgttgt
    21961 atttgttgct ggttagtggg ttaattttag ggttgatgca ttggtcgcga ctaaaagaag
    22021 gatattttgc ctctgtttat tggcgtcagt tacttgttcg aatgttaatt gacaatcggt
    22081 ttgtttatac aaagaaacaa gttagtgaga aacaaacaag ggaaaaaatg agattaccaa
    22141 aagtttattt ttatcaaaag aaacaagaat tagtggtttc ttttccttta gatggtgggc
    22201 gttttcacga acgattttta aaaatgggtc atttgttatc tgaagtattt ttgcgtgatt
    22261 taattcgtga agaacgagaa aaagcccgaa tcaattatgt attcctttca gattctggtc
    22321 ggattcctat tgatcaatgt atagcagaaa atggacgtat tcatttaatg aaaactttgg
    22381 attgggtata tgatcaatca ccaaatatgt tgattgctgg tgcaatcggt ggtgggaaaa
    22441 cctatttatt gtattcttta attcaagctt gtttaagtgt tgggacagta gatatttgcg
    22501 atggaaaagc ggcagattta gcagcattag gtgacgtcga tgtattcaaa ggacatgtgt
    22561 tttacaaaat aaatgaagaa atgattattt gtttacgaaa tgcattaaaa gaaatgaatc
    22621 aacggtatgc ctatatgaaa accatgaaag atcctaaata tgaaccaggg aaaaattatg
    22681 cttattatgg gttaccaccg cattttatct tttttgatga atgggcagct ttttatggtt
    22741 cgttggatat ggaagcgaga aatagagtcg ataaattgat tcagccatta gtattaaagg
    22801 gtcgtcaagc agggatgtac attattttag caatgcaacg accagatgca gaatattttc
    22861 caagtggtgt tcgggataat ttaacattcc gtatttctgt tggtcgattg tctcccattg
    22921 gttatttaat gacttttgga gaagatttta aaaataagcc attttacaac aaagatcaac
    22981 ctggtcgtgg atatgctttt ccaggatttg atgtacctgt agagtttttt gcgccatttg
    23041 tttacaaaga ttatcacttt atcaaagaat ttgagaaatt tactgaaatg attgtctatg
    23101 attttacagt aaaagaggat agtcaagatc aaacgatggg agatgataat cagttgttac
    23161 aagaacaact agagcgccaa atgtatttag caaaagatcc aaaaggaaca agtttttctg
    23221 atgcgcatat tttgtaatca actaaggagg tgaagttaaa tggcagagag ttgttattat
    23281 agagtagcta cagcaatacg aatgattaat ccttctcttt cttccagaac gttttatgat
    23341 tggttgaatc gtattgaaca agtaactgat taccgctttt tgcgcaaaga acgtgttttt
    23401 acaggaaaag ttattaatca agtattgcta acgaaaaaag atatagaacg attgactcga
    23461 ttgtatcatt atcgagtaga tttagaagag gatttaactt tgtcaattta tcgcgttttt
    23521 tctcctgaaa aatatagtga aataacaaaa ttagatcatt taattttgta gttattatct
    23581 atcgggaggg ctctagacgt agtctggtag ccgcactctg cctggcggtg cgcattaagg
    23641 ctgcgaagca tgggcctata atgcgcactc gctagcagcg tgtgcagctc acaaagaggt
    23701 cgtgcgcttg cgcacggcct ctttgtgtaa agccccccga tatataatat ggggggcaga
    23761 aataacataa cacatagaaa aaagttttat aaaccctttt gtatcaagtg attatctaag
    23821 gtcagcagtt gtactacttt tcccaaaaaa cgagggggac tagttgatat atcggtgttt
    23881 ctaaagggtt aaaaaaaatc gattttgtta ttttataaaa ggagcgagaa aatgacaccg
    23941 attgagctaa aaagctacag aaagaagtta agtgcaattc ataatataag tggagataaa
    24001 aagccgctat cacaagcaag atttgcgcaa ttgatgggct gctcaaaaga ttacattaga
    24061 aagatggaac aagggaaaag aaaaatccca gaggattttg aacaacgaat caaggaacgc
    24121 tttcgattgt atatgaaaag cggaatagat cttcgagcaa tgattgatta tttgcgtctt
    24181 tccttttttg ggacagacta caaaacagtg attcaacgtg tattacgaat ggatctgaca
    24241 gaatttgaaa cacgtccaac gggcttgtat ctttttgact gtctttctgt tcgtgggaat
    24301 atctgggtgt tctggcattc aaaacgaact gtaacagaaa atgtacttgt gcaattttca
    24361 ggacaaggtt gccgggaata tgaggaagtc atgcgtgaac aacaaacaga ttggcgaaca
    24421 tggctatatt ccatttggaa aaatcaagga aagttggaag ctggttatac acgtgtgcaa
    24481 tgtagccgat tcgatattgc gttagatgaa ctttggggat tggaaacgga agaacatttc
    24541 gatctctttg aattactaga aaaacaaaaa gcaggtttat tagaaatgga ctttaagaga
    24601 ttccagaata ttggcggttg ttttttagat gatggctatt ttcgaagtga aggattatct
    24661 ttgtattttg gatcgcgaaa gagtcctctc ttttttaatt tttatgagaa acgttttgaa
    24721 attgcgaacc gagaaaaaat ttcagaatta gaagcattaa cgaaatatgg tatttataat
    24781 cgatatgagt tacggttagc caatgagaaa gcaacacaag cagtagaagc atttatttta
    24841 ggtgattcac cgaaacgttt aggagagatt ggcgttggtt taattaatgc aaaacttatt
    24901 gtttgggata gactggaaag tgatgcacgg attgttgata aacgatgggc aaaattattt
    24961 cgttcgatgg aagaattgga ttttacagta aagccagaaa aattctcatt agatcgttca
    25021 gaacgctggt ttttgaaaca agtagctccg acattaaagt tgttacaaga agcagatcgt
    25081 attttaggaa gagactttgt acaagaaggg attgataatg ccgaactaaa tgacgagcaa
    25141 caaaactatc tagaatggat gggaacgtca ggagggaaaa ttgcatgaat gcatatacga
    25201 caacagaatg gctgcgcctg ttggatttaa aggctgcaat cgaagcatta aatgaaaaga
    25261 tggttgattt gtcttatttt cgttttcgag ttccttatat tgaacaagcg gtaaaagctg
    25321 gacgttatca ggaaaaagag aactggcaag aaattgcacg cttattagaa gttcgtaagg
    25381 gatacgaaca agaactcgaa gagttggaat ttagccgaag gaaaggaagg ctagaattca
    25441 ttcgctttta tcgattttca ttgccaatac ctgcgatttt agcggttaaa aaaggctgcg
    25501 ataaaatgaa gatatatgaa aactgtgtgg cagcacttag cagcgaaaaa cctcttatgg
    25561 aagaaatttc attggtgact gtcacttggg aaatgagtcg ccagccaaca gaagaacaga
    25621 catatctttc tttagaagag attgagatag aattagagga aattggtcgc tatgccactt
    25681 gttctaccta ttgtggatcg gtcattagta ttgctggtgt gattgtctaa atgaaaaaaa
    25741 ttatgattta tgttggtgcg tattggtcac gtgatccaac agtattagaa aatccagagg
    25801 cggtacacta ttgcttgcgc caattattct atctgtataa agaacgatta gaatcgttga
    25861 ttcgtcagtt accatatact gacagacgtt tagatgaact tcttctaagg tatccagcaa
    25921 tgtataaacg acgaaaaaat agattgttac cagaagagta tcccattgaa aaacatgaac
    25981 ttgaagggcg atttgtggct tatttctatg atgatgttcg tatgcgctta gtagaacaac
    26041 gaatggaaat agaaaatgat cgctactatt ttatcaatta ttgtaagaag cgtaagtatc
    26101 aagtaactga tgatttttat gattgtattt tacaagatgg ggaagtgatt ctatccaaaa
    26161 ttgctccaag ttttcgtgaa cttgtgccga ttgatttttt gaaaaaatgt catttgcgga
    26221 ttcttccgta aaaaagagct ataaaaggaa agaaaagtag aagccaaatc ggtttctgct
    26281 tttttatttt ttttaggaag gagaagatgg tatgtcactg gaatttttag gagagccaat
    26341 tgaaaaggaa gagttcgttt cgcattatat ggatttgttt attcaggtga taaaaaactt
    26401 tgtgcgattg atgaatttta tccacgcgaa caagactatc tagccgctga acgaataaaa
    26461 atcagtttat tatgggaaaa attcacagat gaaaataacc gaccgccaga ttatcgctat
    26521 ttatcagatt gtttaacgaa taatgtgctt tctaaagaat acctatttca caaacgtgag
    26581 aaaaatcaaa tgtcggttga acatttttgt gattgttata ttgcattatt gcgtagtcaa
    26641 cagttattaa cagtagaacg atatggtgca gagtttggtt atgtcttgca agctgagcgt
    26701 catagagcta caagatgcta tgaaaaagaa gatcaagtga ttgatggcta tgaagaatta
    26761 tgtattcgaa acaatcgcta tttgcaacaa cgtttatatg aacaattgat gatggatttt
    26821 tcaaatctct atagagggta tgaatgttcg atgattcaag aaaggtagaa gaaaaggggg
    26881 aatttttatg tggcgattat tatttttggt ggtatgtggt atggcattgt tctatgtgta
    26941 tcgtgtctat ctctatccac gaatggaacg atggacaagt tggtatagag atcctttaaa
    27001 acaacgaaaa ggattgaagc ggtatcgtta tgaattactt gttgaatttt cggatgggcg
    27061 acaagcacag tattattttt ttgcaaacta tccaaaatac aacaccagtg aactattgat
    27121 tcaattattc aaaaataatc catatgttgg gttagaagaa aacggacggg agttctttta
    27181 tcgttcagat aatattagtg cggcgtctat gaacaaaaca cgaattgcat aaaaaattgg
    27241 aggaataatc atgaaaaaaa caacattagc agtcgtaaca acactaggaa tttcattatt
    27301 ttcgtttcaa ttagggagtt cattggttta tgcagatgaa actgtagaaa cacctaagtc
    27361 agaaattgta gaaacattgc caggggaagg attaccagat gttgagacag agctaaatgg
    27421 aggaacaacg gaaaaaccag cagaacctga acaattgcag tcagagaaac ctgaaaaaga
    27481 gccagtgaca ccagaaccag agtttcctga atcaacttta ccgactgatt cgatcgaaga
    27541 agaaggtgtg catagttcaa caacaacgga ttcaagtata gtcacaactc cggaacaatc
    27601 aggaacaacc agttcttctg aaaagaatac tgattcgtca agtacggaac agccaacgat
    27661 gtcgtctacc agtgaagaac aaaaagaaga accgaaacaa cctgttgaaa aatcagaagc
    27721 aattccaaaa gaaaataaac ctattgttcc agcaaaagaa gtcacagtat cggtaacccc
    27781 aagcggtgaa attacgacga atacttctca aggaatgagt gtaccgattg tcactagtaa
    27841 tgtagaggaa ttgacccata ttccaacacc aaccacccca ttaaaagtag caggtgatca
    27901 aacaattgtt ggtgtaaaag atggtattcc attaatccaa gatagtgaag ggaatttggt
    27961 aaaggatttg tcgattcctg taaagaaact tccaagtggc aatattgaaa tcaaaacagc
    28021 agatggaaaa acaaaagtct taccaaagac tggtgaagaa atacatgttg cactttctgt
    28081 tctaggagga gtgttgacaa gtattgctgg atttgttgga tataaaagaa agagaaaagg
    28141 aatagaaaat gagggtaatt gaatggacta tttttcagaa aaacaagctt atcgttgtca
    28201 atcgtacgta ctagtaaaaa catactagaa ggagtagctt atgaaaaaac gacaactacg
    28261 gcggaaagaa aaagtttata cagagaaagt accgaagcaa aagaaaatcc attcaaaaaa
    28321 agcagtgaaa tggatttggt gtttggtttt atttctttgt ttttcagggg gcttggcatt
    28381 ttttcaatcc atgaaaacga aggttcaagt gtcgtttttg aaagatgaaa tccaacaatt
    28441 acaaaagaaa gaagtacaaa gtcggtcaga attaacgtac acacctgaaa ttgaatcctt
    28501 tatgaatcgt tttgttgctt tgtatatgaa tacattcgat aatcatgaac aagaacaaca
    28561 acgtcaagaa acattaaaaa aagagtttta tgcacaaaac tttattcaag aaccaggaag
    28621 tgtttcaggt gttcgaaaat taactgctgc ttcgtttatt ggattaaaag aaattgaaac
    28681 cattccaact gctagttata aagtaactta tgatattcgc tcgatgaatc cagagagtaa
    28741 gaaagaaaca agtgtgacaa tcacacaagt actcaatctt cccttttcta tttcaaacaa
    28801 gggctgtcgt attattgctt atccttattt tacggcacta ccaaagaata cagaaaaaca
    28861 acgaatgatt acttatgatg acacattata cgaaacggtt gatgcgtcgg aagcgaaaga
    28921 tgttgaagga tttgtggaag atttcttaga taagtatgcg aaaaacaatg caaaagatat
    28981 gtcatacatg atgaaagaac ctgaaggact caatcaaaca gctaaagttg atcaagtaga
    29041 gatgaaagta ttgcgttata aagaaacgaa aaaacctttt attgtaaaag caacggtcgt
    29101 ctttcgagat ggtgatacgt cacttttaca aaaagaacaa atgacgattg tcattacgaa
    29161 aaaaacaaat cagtattatg ttgaaaaatt aacacataat tggttggata ggaaaggaga
    29221 agaacggaaa tgacactgaa gggaattgta gattttatat taacgcaagg tggctatttt
    29281 ttaataatgg tggttgttta tctgggattg cgaaatttta tgaaatcaaa agtaggaaat
    29341 gcattaatat ctatcgcagc aggtgccgca gtttatttct tcttaaatga tccaacacgt
    29401 gtatttgatg cgtttggtaa aatcattgaa aaattattta tggattagga ggatagtgaa
    29461 gtggattggg aaccttatga ttattcgatt gtttatgcgg aaccttatcg cgtacaacag
    29521 attacaagca attggcgtct tccatttcca gttgcattag tcaatgcagc aacttttttg
    29581 ttggtcgtaa tctttgaata tttattttta cgttcattta ttggttgggt catttctttt
    29641 tttccaccag caggagttgt tttttatata ggactcccct atttatgtgt gttagcactg
    29701 gcaaaactaa aacctgatgg aaaaaaagtg catttttatt tatgggattg gttttgttat
    29761 atgattcgta tgatgaatgg tgattgtgtg tattataact gggacaaggt atcaaacaat
    29821 gcattggaag aattactttt tgtgaataag tatgtttatt tagaggatta gaaaaaggga
    29881 caatcaactg gttgttcctt ttttattggg aggtgaaatt cattggtgct tgtaaaagaa
    29941 ccagtaaaag cattcaagaa aaattgtttg ttaaatggag aggattgtat ctgggcctac
    30001 tatcatgttt ttccacgaac aatttcaaaa aacacaccaa aaaagacaca ggaaataaaa
    30061 gaaatgatgg ctcgtttact gttgctttta gcagattatg aagatgtgga gttggtgatg
    30121 tatccacgaa caatgaattt acggacgaag tttgacacat taggaaaggg attttctccc
    30181 gatagtcaag caattggtgc gtattatgca aaagaaacag tgacattgtt ggaacgagaa
    30241 ggtcaagtct atcgtccttc atttatttta ggaatcaaag tcaaagaaaa agcaacggtg
    30301 gagagttttc atgatgtaat aaatctaatc tggttagatt tagcaggaag tttagcacgc
    30361 ttatttggat tagaagtaga tgatttggaa actttgtttt ctgaaagtga tccttatgta
    30421 aaagaagtga cacaattact ttatagcttt tctattcgtc ccttagtaaa aaaagagctg
    30481 ttgtatctca atcgtctgca atatattcga ggaatggctc acaaagtaga agaacaaaat
    30541 aattgggaag tactgaaaga caatcaagga acgtatgtac gtataccgaa ggaacataaa
    30601 ggccttcttc aattagaaga tgagttaggg agtagttatt tagcattgtt gcctattggg
    30661 gaaacacctg aaaatatgag aaattgtgat ctatttcaat ttgctcagga tctttctttc
    30721 ccagcagaat tgcgaatgaa aattcactat ccacgcttga aaggacaggg aggtattggt
    30781 ttaaaactga attggctatc ggaacggttc aaaacagagc ataaggaatt ggttcaaaat
    30841 gatgaagaac ctagtgaacg cttattaact gttcgaggat tagtaaaaca tttaaagcaa
    30901 cgagtagata atgggcaacc tgtcgtcgat tggatcgcat gttttattgt atacggtcaa
    30961 acggttgaag aagttcgtca aaatagtgca acggttattc aacaacttca tacgattatc
    31021 catatccacc gtgcaaaaaa tgatcaacga gaattgttct ataaatgttt attagggcaa
    31081 ccattgggac ggcagttaca ttggaagcac cgaacaactg tagaagcagt tggcgaatgg
    31141 ttgttttcag cagtatcaga attaggtatg gaatccggct ggtatattgg tcggcaagat
    31201 acattagggg atggtggttc tcgaggagaa aaaacaccat tagaagaatt aatttatgcg
    31261 tcaaatcgtt ttgtcttttt gaatcttcta gccattgcgg aaggaatcaa aggtgcgttg
    31321 tttgatgcgc ctcatgtagc agtaactgga aaaaccggta aagggaagac ctttttagtt
    31381 ggcttgctct ttttgtatag ttcgtttatg aatgtacaaa gtttatttgt ggatcccaaa
    31441 ggagaaaagc gcaaatggtt tacgaaactc attcaagacc cgtattatca gaaacattac
    31501 cctttgtttg tagaacattt gcaaaaaatg agttatgtca cgttagatgc tacaaatccc
    31561 cgtaattatg gtgtattaga tccaattgtt tgtttatccc gagtagatgc gaaacaagta
    31621 gcccaggata tgattgatga gttaagtccg ttaggtacca atcacctttt aaaaggggcg
    31681 gtattaaagg gaatccaaga agttttagtt caaaaagaag ccggacaaca agttggcttg
    31741 atgcatgtgg tcgactattt agaacaaatg gaacaaaaag aggttcgaga atatggtgag
    31801 tttttacgct tgacggtaga agattcgatt ttacgtttag gattttctta tggagaaaac
    31861 ccgggattag actttaatgc caaaacaacg attttagaga tccaagattt gaaattacca
    31921 aatgacaatg tccgaccaga actatataca gatgcggatc gaaagtcttt gtgtttgatg
    31981 attagtttag gtcgtttttg tgaaatgttt ggaaaacgag atagtagtaa gaaaacagcg
    32041 atttatttta cagaagcatg ggtatttaat aattcgaacg caggacgtag tattattgca
    32101 gcaatggcac gtgtggggcg ttcccagatg aatcaattag tgttagatac acaatttatt
    32161 ggtgacttag gttcagataa agaaaaaggg aattttggtg tcgtctttgc ttttgatgaa
    32221 gacagtgagc gagaaaagat tttgaatcat ttacgtttgg aagtaaatga agcgaactta
    32281 gctgaaatga gccacatgat taaaggacag tgttggatgt tagatcccta tggacgtgta
    32341 ggaaaactca atgtacacag tttgtttgaa gaaatgaccg ctgcattaca aacaacggaa
    32401 aaaacaagtc actctcaagt agaagaacag gatacgttaa tcatgcaata gaaatgaggt
    32461 gaaacaatgg cttatataaa gcataaggaa aagaaatggt gcgttttgtt ttttttaacg
    32521 attggattcg gactgctttt tgttggtgga ttggtgtatg cggcagaaaa gcaggtggat
    32581 caatcgatct atgagattgg gaaatttcat attgatacct tttcttctta tttatctgat
    32641 agtgaatttt cattaggcaa aatgttattt ggggcaaatg gcacagattt tctaaaaatg
    32701 atgacgaata tgttctttag tttaagtaaa ttgatttggc aaggccttga ttttgtcatt
    32761 gagaagctct atgaaggagc tgcaatgaac gagttaattc accagttttt cactttttcg
    32821 gatactattt attcaaaaat atacgatcct attggtgcgg ggattgtcgt cttgtatgtg
    32881 ttatatctat ttactcttcg tatatttaga gggaaacatt acgcaaaaat ggtgtttatt
    32941 cgatttctct tcgttgtcat tttttcaatg gtttggtttg gctatggtag taacgtacct
    33001 agtaaaggag aagcttttac caaagggtta aatgatttgt ccgtggaggt agaaggcttt
    33061 attttttctg ctacaaatag tctagatgaa ttggcgacaa aagatgtcaa tggtgtttta
    33121 gtgccaacga cacaaaagga agccattcaa aaagtgcgcc aactttatta taaagcggct
    33181 gttgttgatc cgtatatatt gttaaattat gggacaacaa atttagaaga attgtatcaa
    33241 gcagagattg aaccacaaga atttctagcc aaggaagcgt caagtgaaga attatctgat
    33301 attgagacaa aagtgaccac tgctgcagga gaagatagag ggaaaaagaa ggcttatcgt
    33361 gtctatattc aaccaactaa agctgtttat aaagcagtcg ttggttctat ggtgccattg
    33421 cttaatctaa gtattggatt accacttgta gtgattggca tgattcgttt tatctttcaa
    33481 atgttggtgt tgtgtttatt acttgtgatt cctttttcat tactcgcttc ttttgttcca
    33541 gtatttgatt atatgctcat gaatacctta aagtcctttt tgggcttgat gtttcaaaaa
    33601 tctttttaca gtgtgattat tttggtggca tttttgatct ataacatagt agacgattta
    33661 attccaatga atactgtttt tggtttttta ggaaatatgt ttgtcaaagc actcctatca
    33721 attttactca ttgtaaaacg gaaagattta ttaaagaaac tagggttagg agctgcagat
    33781 caaactataa acaacacgaa aaacatagca agtcattctt tgtctcaaat gaaacaactg
    33841 cctggtcaac tatcaactgc tggacaaaat ttggcaatca aaggtgcagg attagcaggg
    33901 aatgtgtatc gagcaacgaa tccactcgct tctgcagcga tacatggatt tcaaaagttt
    33961 caacaaaaac gtacaccaca agaatcggta aaagggaggc aagtggttaa tccctttgaa
    34021 ctaggtgggc gttcaaatca agccttgcct tatagtggtc gtcatgttcg ttatcatggg
    34081 gtagagcctg aaatgtcttc taccaaatcg aaaacagagt ggaattctgg tctttcaaat
    34141 ggtttgattc caaattcgtc catgaattca gtaagaccaa aacaacaggt acaatcattg
    34201 gcacaattga gtaagcaaca agggaataat caaagaaaac cattgttaaa taaaggtatc
    34261 gcttttgttg gtcaatcgat tccgttgcct tctggaaaag tacccgtgcc acaattaaca
    34321 gctgctggtt ctctttcaca acagattcgc caagtgaaaa atcaggcgaa aaatgggagt
    34381 tatccagcaa tgcagcagtt attgaaccaa tactctgttc ctaattcgtc ggtagccact
    34441 ctatcaggaa atcgttctgt gcaacaaccg gggcaacgtt cgtctgaaag taaaccgtcg
    34501 atttccgcac caaaatcgtt acaacctgca ttttcttctt tagataagcc aacagagcaa
    34561 tcggaaaaaa tagaaaagga tcctaaaaca ctgccgattt tggacgcaaa accaggaact
    34621 gattttactt taccaaaagg acgaacagtc caaaaaccat tatcgaagaa aggatgagga
    34681 agaaatgaag aaagggtgtc taggttgtct tcttttcttt attcttccct tttttctatt
    34741 gccattcttt ttttcaggag aactttctga gcatggagga gaagctacag gtctagctgt
    34801 cgcttttaat ggggaatata ccgaaggatt accgattttt tcagagatta aaggacgtgg
    34861 tccaatcaca gatgaacatg cgcaagcagc agttggtgca gctgtaaagt atcagttatt
    34921 accgagtgtg attctttctc aattaggcta tgaatcagct tatggacagt cacaatctgg
    34981 aagaaacgat acgaactttt ttgggattac ctggtttcct ggttgtccct acccacaagg
    35041 ttctgcacgt ggaattggtg gttctgaagg tggtttctat atgaagtttc cgaacttaaa
    35101 agcttgttat gactattatg gctttatggt ggcgagccaa agcaacttta atcaatgtgt
    35161 gggcaataaa gatcctggaa gttgtttatt gattctaggt cgtggaggct atgcggcagc
    35221 aggtattacc gaagggagtc cgtattatcg aggagcaatg agtatcataa cgtcctataa
    35281 cttaacggaa tatgatactt ttgcgatcaa gcattggaat agcctaccaa caaaaaaaac
    35341 ttcgcttatt gcaggaaaaa gtaaaggcaa tgcgaaggtt ttggatcgga tcattggacg
    35401 atccgtaaat aatggggaat gttatggtgg tacggcatgg tatgtatctc aattaggtgg
    35461 cccacagtta atgggttctg gacacatgtt tgcgatgtta ataggggaag attataactg
    35521 gacaacctat ggttggcaag taatcatgaa cccaaaacca agtgatttac aagcaggaga
    35581 tgtggtgaat tggcaagcag gtggtgcatt gtctcctgga atctatggtc acaccggaat
    35641 tataacagat gtttcatatg gcggacaaca tttctctagt gtggaacaga atgccgagca
    35701 aggacggatt atggctcggt atcaacgcac gtacaatcaa acaaaaattc gtagtctggt
    35761 tcgtaaggtg aaataagaaa ggcagtgact agaaacgtga aaaaagggat aattttagta
    35821 tttagttggt tatgtttgat aggtcttact ggttgtcatt ggttttcttc tgaaaaacaa
    35881 aaaacacaat tgagaacgac agaagaaaca atcgaagcag taaaagaaac aagtggaact
    35941 cttcaaagta ctgaaagtaa cagtcaaatt ggttcattag aagacgagac agcgcaacaa
    36001 atgcaaatag atgaaatgac aaaaaatttc ttaaaagatt ttgctagaaa atggagtaat
    36061 ttcacagatg tttataaacg caatcaagct gtgagggact atatgacaga tcgttgtatt
    36121 aaagacaata gtattgatgt agacccgcat gtacaaatga aaacaactgg gaaactttac
    36181 caggtgacac aagatataga gaatccggat caatatttgt tatttggaga agaaacaatg
    36241 aataaggtta ctcaatttgt attacttcaa ttgaaagtag atcaagaagc tcaaaagatt
    36301 gatgcgatca agatttatta tgtacggagt gcgtattaaa ataaaaaatg aacactcata
    36361 tttattatac accgaagaaa tagaaaaaaa cagactgttt ttttaagaaa aactatgtca
    36421 ttctttactt gaaaaaagtt gttataggac agaaaggata aaacaaaaac ggaaaaaata
    36481 tgtcttatga ataactataa gaactattct attttttagg agatggatac gagatgaaaa
    36541 aactaattgg ctatagtgtg atgtgtattg gatttgttgg tgtgggattg tttgtatcga
    36601 ctgaatctgc ttctgcggct gaatggtata atcctatctg gtataatccg aacattgaat
    36661 gtcaaaaagg ctatccgtat ggaccgacaa atctgaaaaa tggaaaatgt agagtaaatt
    36721 ggaatggcgt tgtagatagt gtaattactg gttcaatgga ctctgctcta catggcttta
    36781 cgggtcacta gaaggaggaa gaaaatgtct gaagagaaaa aagaaatcgt tgaatcatta
    36841 gtagctttaa aagattctct ttctaagatt gaatatgttg ataggcaaga aattcaaaaa
    36901 ctaattgatg atactattat tgaaatccaa gatgcgcgtt gtgaaggtat aaaaatttct
    36961 gtagctttat ctaaagtaat agaaaaaatg aaccgttcat tagcgtttaa tggattgaag
    37021 ctagatagac aaactagtct gatatgggat catttaaaag acttgtatga caagtctaaa
    37081 agaagtgaac ggactgctgt atcaatctta aaaggccttt ggggaatgaa ttcataaaca
    37141 agatatttaa aaagtaactt attttttatg gaggagaaat tatgaaaaga ataattaaaa
    37201 tatttggtgt tttaggtgtt ttcttattag gtttaagtac ttttgttagt actggaacaa
    37261 gtgtgtatgc cacagaaaaa gtgaatacag ttgatttaga aataaagaat aaattagtag
    37321 atgaattaac tttttatttt gatacagttg gttttttaga cgaacatggt gtataccata
    37381 taaaggattt tggtgctatt cgtgaacgaa tcagtcaaga taacgaacaa ggaagagcag
    37441 caagagtctt ttatgaacat attattgttc ctagaatgga gaaagttgat tggaaacgat
    37501 atgctggatg tgtagccatt aattctcttc ctggaggagg agtggcttgg gctataggta
    37561 cgcagggaga ttttattgaa gcattaaaag cttttaattt tgaaaaagct gcggatttat
    37621 tattaaaaat tgcagaaaaa caattaggaa aagctgcagc taaagagtta gctaaggtaa
    37681 atattgtagc aggaattgca gcagctgctg ctagctgtgc attagagtaa aagtttatga
    37741 aaagtttaga acaagagcat atggctttta aacaagcaat gtttaaagaa aatatctatt
    37801 taaaccataa ctatattcgt gtttctaagg catgttcacc agtattgaat atgcttggag
    37861 gtggaaatgg attatatcat ttactgtttg tcgatgtttg ttggttaatt tttttaccag
    37921 atgaactagt tatagtcaat gaaaaaataa caagtaaaaa tgaagtgttt aattattctt
    37981 taacaagaat taattacaaa gagattacca agttttcact agaaaaagtt cctttttggg
    38041 gagaattttg tttaaaaatt aaatgtaact ggaaaaga
//
LOCUS       DAZKSU010000021        37165 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000021 DAZKSU010000000
VERSION     DAZKSU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.021"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            647..964
                     /locus_tag="ACG8Z3_001090"
     CDS             647..964
                     /locus_tag="ACG8Z3_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0823424.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="ACG8Z3_001091"
     CDS             complement(1055..1420)
                     /locus_tag="ACG8Z3_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823425.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="ACG8Z3_001092"
     CDS             1628..2110
                     /locus_tag="ACG8Z3_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823426.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /locus_tag="ACG8Z3_001093"
     CDS             2307..3197
                     /locus_tag="ACG8Z3_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0823427.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(3484..4758)
                     /locus_tag="ACG8Z3_001094"
     CDS             complement(3484..4758)
                     /locus_tag="ACG8Z3_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0823428.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            5031..7502
                     /locus_tag="ACG8Z3_001095"
     CDS             5031..7502
                     /locus_tag="ACG8Z3_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0823429.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(7605..9341)
                     /locus_tag="ACG8Z3_001096"
     CDS             complement(7605..9341)
                     /locus_tag="ACG8Z3_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0823430.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(9338..10042)
                     /locus_tag="ACG8Z3_001097"
     CDS             complement(9338..10042)
                     /locus_tag="ACG8Z3_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0823431.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            10173..10676
                     /locus_tag="ACG8Z3_001098"
     CDS             10173..10676
                     /locus_tag="ACG8Z3_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0823432.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            10636..10974
                     /locus_tag="ACG8Z3_001099"
     CDS             10636..10974
                     /locus_tag="ACG8Z3_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0823433.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            10995..12413
                     /gene="ffh"
                     /locus_tag="ACG8Z3_001100"
     CDS             10995..12413
                     /gene="ffh"
                     /locus_tag="ACG8Z3_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0823434.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            12525..13103
                     /locus_tag="ACG8Z3_001101"
     CDS             12525..13103
                     /locus_tag="ACG8Z3_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0823435.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            13107..13628
                     /locus_tag="ACG8Z3_001102"
     CDS             13107..13628
                     /locus_tag="ACG8Z3_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0823436.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(13707..14261)
                     /locus_tag="ACG8Z3_001103"
     CDS             complement(13707..14261)
                     /locus_tag="ACG8Z3_001103"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0823437.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            14514..14789
                     /gene="rpsP"
                     /locus_tag="ACG8Z3_001104"
     CDS             14514..14789
                     /gene="rpsP"
                     /locus_tag="ACG8Z3_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0823438.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            14801..15052
                     /locus_tag="ACG8Z3_001105"
     CDS             14801..15052
                     /locus_tag="ACG8Z3_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0823439.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            15177..15968
                     /locus_tag="ACG8Z3_001106"
     CDS             15177..15968
                     /locus_tag="ACG8Z3_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823440.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            16138..16659
                     /gene="rimM"
                     /locus_tag="ACG8Z3_001107"
     CDS             16138..16659
                     /gene="rimM"
                     /locus_tag="ACG8Z3_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0823441.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            16649..17410
                     /gene="trmD"
                     /locus_tag="ACG8Z3_001108"
     CDS             16649..17410
                     /gene="trmD"
                     /locus_tag="ACG8Z3_001108"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0823442.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            17546..17893
                     /gene="rplS"
                     /locus_tag="ACG8Z3_001109"
     CDS             17546..17893
                     /gene="rplS"
                     /locus_tag="ACG8Z3_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0823443.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(17929..18114)
                     /locus_tag="ACG8Z3_001110"
     CDS             complement(17929..18114)
                     /locus_tag="ACG8Z3_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823444.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            18151..18444
                     /locus_tag="ACG8Z3_001111"
     CDS             18151..18444
                     /locus_tag="ACG8Z3_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0823445.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18532..18678)
                     /locus_tag="ACG8Z3_001112"
                     /pseudo
     CDS             complement(18532..18678)
                     /locus_tag="ACG8Z3_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            19287..21614
                     /locus_tag="ACG8Z3_001113"
     CDS             19287..21614
                     /locus_tag="ACG8Z3_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0823446.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            21611..22525
                     /locus_tag="ACG8Z3_001114"
                     /pseudo
     CDS             21611..22525
                     /locus_tag="ACG8Z3_001114"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            22598..23113
                     /locus_tag="ACG8Z3_001115"
     CDS             22598..23113
                     /locus_tag="ACG8Z3_001115"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0823447.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            23250..24539
                     /locus_tag="ACG8Z3_001116"
     CDS             23250..24539
                     /locus_tag="ACG8Z3_001116"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0823448.1"
                     /translation="MKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKD
                     TAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLPF
                     YNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQFVT
                     GFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQKET
                     TFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHFIKQ
                     DVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKLISD
                     SEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQPKG
                     ILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            24745..25917
                     /locus_tag="ACG8Z3_001117"
     CDS             24745..25917
                     /locus_tag="ACG8Z3_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0823449.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(26079..26753)
                     /locus_tag="ACG8Z3_001118"
     CDS             complement(26079..26753)
                     /locus_tag="ACG8Z3_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823450.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            27124..27546
                     /locus_tag="ACG8Z3_001119"
     CDS             27124..27546
                     /locus_tag="ACG8Z3_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823451.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(27712..28905)
                     /locus_tag="ACG8Z3_001120"
     CDS             complement(27712..28905)
                     /locus_tag="ACG8Z3_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0823452.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            29129..29506
                     /locus_tag="ACG8Z3_001121"
     CDS             29129..29506
                     /locus_tag="ACG8Z3_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0823453.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            29494..29832
                     /locus_tag="ACG8Z3_001122"
     CDS             29494..29832
                     /locus_tag="ACG8Z3_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0823454.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            29966..30409
                     /locus_tag="ACG8Z3_001123"
     CDS             29966..30409
                     /locus_tag="ACG8Z3_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0823455.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            30671..31486
                     /locus_tag="ACG8Z3_001124"
     CDS             30671..31486
                     /locus_tag="ACG8Z3_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0823456.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            31496..32158
                     /locus_tag="ACG8Z3_001125"
     CDS             31496..32158
                     /locus_tag="ACG8Z3_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0823457.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            32363..34027
                     /gene="alsS"
                     /locus_tag="ACG8Z3_001126"
     CDS             32363..34027
                     /gene="alsS"
                     /locus_tag="ACG8Z3_001126"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0823458.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            34040..34750
                     /gene="budA"
                     /locus_tag="ACG8Z3_001127"
     CDS             34040..34750
                     /gene="budA"
                     /locus_tag="ACG8Z3_001127"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0823459.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            34880..35377
                     /locus_tag="ACG8Z3_001128"
     CDS             34880..35377
                     /locus_tag="ACG8Z3_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0823460.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            35562..35822
                     /locus_tag="ACG8Z3_001129"
     CDS             35562..35822
                     /locus_tag="ACG8Z3_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823461.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(36184..36423)
                     /locus_tag="ACG8Z3_001130"
     CDS             complement(36184..36423)
                     /locus_tag="ACG8Z3_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823462.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            36709..37023
                     /locus_tag="ACG8Z3_001131"
     CDS             36709..37023
                     /locus_tag="ACG8Z3_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823463.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT        12490 a         6474 c         7373 g        10828 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgcatatt
     3481 tttttagttt tcaaagatca gaccttgttg taaatagatc cgtgcccgaa agttataaaa
     3541 gttgcgataa ccaaacgcaa ttcgtttaat cagcttgatc ttgttattca gcccctcagt
     3601 gacgccgttt gagtgggaca ccttaaaggc atttgaaata cccgattggt agcaatggaa
     3661 gatcgtgaat ttctttttaa acgactgagg taacgtagat gaaattgttt taagggtttc
     3721 ttgaaagcct tccgtacctc gtttaacgaa ataatagcgt aataattgac aaaaatgata
     3781 cgcagttcgc aattcttcgt tgtagcttaa cagctcatca acgatttctg tttgactaat
     3841 tggtcgttta aacagtcgat gataatgccg tttgagtggc tcccatgtcg tggaatcttt
     3901 gagtaagagt ttccaatatc gcttgagccg acggtacttc ttctgatttt cagagtgtga
     3961 ggttcgaaaa cgattcatga tctggacacg taattgattg aagctgcgat tgatgtgttg
     4021 gacaatatga aagcggtcag ttacgatctc cgcatttgga aagactgttt taagcaactg
     4081 acaatagctg gcattcatgt ccatcactag gtacttgacc tttaaacggg cctttctagg
     4141 aaagcgcagg aagtattgag ttagtttgta aagtctgcga tcttcaagga tcgtaaatag
     4201 ctgattggtt gtcccatcaa cacaaataaa actcatggaa ccagaaacag aattcattga
     4261 tttgaactca tccatacaca acaccgtcgg aaggaagttc atacgtggct ggtaatgctt
     4321 cgttgcttca cgcaagatcc gtgtcaccgt cccatccgag acgaagtact tcttagcgat
     4381 tagtttacgt gattgatttt cgtacagttc catgagaatc gcctgcttca gttcctttgt
     4441 tagttgatga tgttcatcaa ctaagtccgt tttcgcagaa aaggtccttc cacaatcatg
     4501 gcagagaaaa cgggtacgtt ttaattctaa aatagttaac ttttctttga ctttcaacat
     4561 ctgagtgcgt gtcgtatagg aaccgttgcg gataatacga accgatcgac agtgttcaca
     4621 ataggaagaa gagtcatcca attttccttt aataaactgc gcttgaacgc ctttatattc
     4681 tttttcaatc agccattctt catcaaaaaa taaatgttta tctgttaatc cgagtaattt
     4741 tctagtatga ttatccattg agagactcct ttggtaggat gacgttttgg tcgactaaat
     4801 tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaagtgttag tagaaaatcg
     4861 ccttcgattt tccaccaaca caaaatatta tagaaccctt tttttttgct atatgacagg
     4921 aatgaaaggt gtttgcaagc ttaatgtgag gaacactttc catatctgtt ctatactgga
     4981 taggagaagt ttggaaatat ccagaaaacg aaatcaatcg aggtagccac atgaagaata
     5041 aatggataaa tcgaagtaaa aaaaagaaag taacacaaag atcatcagat aaagtcatcc
     5101 gtctttttcc atcgtataaa gaaggattaa cggcagatca agtcaaggaa cgaatagaaa
     5161 aaggtgcagc aaataattca gtcgatccca cattcaaaac aaatcagcaa atcgtattgg
     5221 aaaacatatt tacgtatttc aatttgatct ttcttatctt agccattttg ctttgtttag
     5281 tagaatccta taagaacttg acttttttac cagttatcat agccaataca ggaatcgcca
     5341 tttttcagga aattcgatcc aaaaagatct tggacgaact aaatgtttta aatacagcaa
     5401 aagtcaatgt catacgagac gggatagaac aaacaataga tatagaagac cttgtaatag
     5461 atgacattgt tattttagag actggatatc aaattccagc agatgcagag gtcgtggaag
     5521 ggaaactgca agttaatgag gctttattga caggcgaagc agatgaaata accaaagaaa
     5581 taggtgatcc attaatgtcc ggcagtttca ttgtttccgg aaaagcttac gcccgattag
     5641 acaaagttgg agcagattcg tatatttccc agctgacagt caaagccaag acaatgggag
     5701 aaggagaaca gtcggagatg attgcttctc ttaatcaatt gatcaaatgg attgggatca
     5761 tcattattcc tatcggtatc ctgctttttt ctcaaagtta cttttttaat ggaaacacga
     5821 tcaaagaaag tgtcgttgca atggaagcag cattgatcgg tatgatacct gaaggactgt
     5881 acttgctgac gacgattgca ttagcattaa gtgcgactaa actagcaaag caaagagttc
     5941 ttttacacaa tatgaaaagc attgagacat tggcaagggt aaatgtcctt tgtgtagata
     6001 aaacaggaac gatcacagaa aataaaatgt ctgttcaaaa aatgatcgtc tctaaaaaac
     6061 aagaaataat cagtcagtca gatcaattgg aagcatgcat tgctgactat gcaaaagcga
     6121 tggcaggaga caatgcaaca atggaagctg ttaaagcata ttttacaaaa accactgatc
     6181 aatcctatca ccatgttatt cctttttctt ctgtccaaaa attcagcagt gtagcgttgg
     6241 cagacaaggt gtatgtttta ggagcgcccg aaatggtttt gcgcgatcaa ttgccggaat
     6301 atgcggaaga gtttgtttct ttcgctgaaa aaggctatcg cgtgttggtc tttggtgtgt
     6361 acgatgggat tcttgaagaa ccagtattga cagaagcagt cacaccatta ggctatattc
     6421 tgattgctaa tcctatcaga aaagaagcaa gagccacatt tgattacttc aaaaaacagc
     6481 aagtagccat caaagtgatt tctggagata atccgctcac cgtatccaat gtagctgtcc
     6541 aagcagggat ttcacatgct gatcagtacg ttgatgcatc taaattatca gaagaagaat
     6601 atgaagcagc tgtcgaaaaa tatacagttt ttggacgcgt caagccagaa caaaagaaaa
     6661 tcttcgttca attgataaag cgtaacaata cagtagctat gacaggtgat ggcgtgaatg
     6721 atattctagc catgaaagaa gcagactgca gtatagcgat ggcttccgga aatgaagccg
     6781 ccatgcaggc ctcacaagtg gtcttacttg attctgactt ttcacgcatg ccagaagttg
     6841 ttgcagaagg acggcgcgtt gtgaacaata ttgagcgatc agccagcttg tttttagtga
     6901 agaatatttt ctcctttttg ctctcactgt tttcagtgat ctttgctctg acctatcctt
     6961 tagaaccatc acaaatcaca ttgattagtt tgtttactat cggacttcca tctttcttgc
     7021 ttgctctaga agaaaacaaa aagcgaatca gagggaaatt catcatgaat gtgatggaaa
     7081 aagctgtgcc aggtggacta acagatatga tcgtagtcgg tgcgttggtg atctgcgggg
     7141 tgactttgaa tctaaataaa acagatgttt ctacagcatc taccatgctt ttgattgcag
     7201 tagggttcct cgttctctat aaaatctgtt caccgctgaa taaattcaga agtcagatca
     7261 ttcttttctg tgctagcggt aacttttttt cggtcatctt tttgcataag ctgttttcaa
     7321 tcaccggtat ctcagctgta tcacttttgc tgttgagcat tctatttttc tcggcagatt
     7381 cgatttttag gcatttgacc acattggtag aatatttgtt tgaacgtaaa aatgaaaaag
     7441 agcaaaagcc gtttagtcgc gtagctaaaa aatttctatc gatatttaaa cgagaaaatt
     7501 gacgaaaagt atctcaaaaa agagtaaaaa agaacaacca ctcgtgtatc tttcgaagca
     7561 ggatgagtca tccaacttta aaacgcacga gcagttgctc ttttttacat tttcggaatg
     7621 gtaacggtga aagtcgaacc tgttccaaga tgactgtcta cagtaatcgt accattaagg
     7681 agttgcacgt agtcttttac aatcgctaac cctaatccgg tcccgcctga atgtcggctt
     7741 cgcgccttgt ctacgcggta aaaacgctca aagatccgtt cctgatcctc aatgtcgata
     7801 cctataccaa agtcttggac agaaaacgat agatcatctg ttgcctgata gcggataata
     7861 atctcgctgt cgctttttga atattggaca gcattttcaa tcagattttt gataattgga
     7921 taaaggatat ctgttttagt agtaaatggt tcgttctttc caatcaatcg aatcgtcaac
     7981 cgtttctttt cgatgattgg ctgataattt tgaacgattt gctggaaata aggttctgta
     8041 taaattgtct ggatctcgta agaattctcg ccatcttttg acagctggat gatttcccga
     8101 atcagtttat ctaaccggat cgcatctttt tgcatgatct caagaaaaga agtcagagtt
     8161 tgcgggtcat cttttgcacc atccagtaaa gtctcggtaa aaccaatcag tgaagtcaca
     8221 ggagttttta attcgtgaga gacgttgcca acgaaatcct tttgtagctt ttctaaacgt
     8281 cgcactttcg ttaagtcata cgccacccct agaatctgtc cggtaccttc tgaattagta
     8341 aagtagcgca aactcaagtc taatgtctgc cgaccttctg taagggtgat ctcctgatgg
     8401 atcagcggat gctcaggagt cacttgatga atcagctgaa tgagttttgg ctcttgaatg
     8461 acttctgtgt accgctgttc aggttgatag cttaccacgt ttaaatgtag ctgcatcttt
     8521 ggattaagca gtagtaaacg agaatcagca tcatcaatca ggaatacgcc aatcatcagt
     8581 tcgttcagta acgtatataa ttgctcttct gtggtagtac acgcccgata catcttactc
     8641 atttgttcgc tcaacgcatt aatcgtctga tacaattctt cccattgatc gggtgtttgc
     8701 atgattactt ccgcctgatt aggattttgc accatttttt ttagtacggg cagcacggtt
     8761 ttcaatggct ggtttttttg atagatcaga taatagatca ttcctgcaag aacaaggaag
     8821 aaagacagaa agaagaaaaa gacccaacgg cgaaaactct cagttctcgg taaaaaaacg
     8881 cttgtcggtt ctgcgatacg taaaatggct tctaactggc cacttttttt gactggtaat
     8941 gcgacatata aaagatcctc attcaaagta gtacttttgc gcaaagcgct acctaggttc
     9001 ccaccattca atacggcttt gatttcggga cgggagtttc gctgttcatt caatgtttca
     9061 ttgtttgtgt caaataagat ctttccagta gcatccatta aagtaatccg ttcgttcgac
     9121 tgatggacga attcttccaa agcagttttg ttttgcgctg cttggaggtt ttcgacatct
     9181 aattgccgga tcagcagttg tgcttttttt gtcaggtagc tttcttgctg ttccacgatc
     9241 tgctgttgaa aataatggga aatcaattgc cagccaccaa aaaacaaact aactaaaaga
     9301 ataagaaaga agagacgttc ataaattttt ttccatttca tcgtttgggt tcctgaaact
     9361 tatagccaaa tcctctaata gtcaccaagt actttgggtg ttttggatct cgctcgattt
     9421 tttctcgcaa atgactaacg tgtacatcta cgattcggct ttgtcctgcg aagtcaaaat
     9481 tccaaatgcg gtctagcaat gtgtctcggt cgatcacacg gtcttttcgt ttcatgaaat
     9541 aaacaagtaa ttcaaattct ttcggtgtta gttcaatcag atggtcgtct acggtcactt
     9601 gataattggt caaatcagca gtgatttgtc caatcgatag atattcaggc tcagcttcat
     9661 cttttgattg cttacgaggt tcaattcgtc tgaatattgc tttcatacgg gctaatactt
     9721 cgcgaggact aaatggtttg gtcaaatagt cgtcagcacc tatttctagg ccaatgatcc
     9781 gatccacttg gtcatcttta gccgtcagca tcaaaatcgg tgtttcattt ttttcttgac
     9841 gcagccgctg agtgatctcc atgccatcca tcgttggaag catcacatct aaaatgatga
     9901 aatcaaatga ttgttctaat gctaattcta atccttcgcc tccatctgtg gcactggtta
     9961 ccttgtagcc ttctttttcc aaattgaacg tcaagagcgt tacaatagaa ggttcatcat
    10021 caacaactag tatttttttc attcaaatct cctttaaata ggtttcattc tgtttatatt
    10081 ttagcataga atcagtacaa agggctattt tacaaaagaa ataaatggta taataggaag
    10141 gcactttgct gaatagaaag gaagagcagt caatgattgg aatcagtgca tgtttaggcg
    10201 gtctggcctg tcgctatgat ggacagaaaa aagaagtacc agaattgatg gaactagttg
    10261 aaactggaga agctgtcatg gtctgtccag aagtgatcgg cggattacca attcctagag
    10321 atcccgctga aatcattggt ggaaatggat tcgatgtgtg gaaagatcaa gcaaaagtat
    10381 ggacgacaag cggcgaagat gtaacggaag cgtacaaaaa tggcgcgatc aaagcctatc
    10441 aaaaattgca ggaaaaacac attgatctag tcatcatgaa ggaaaaaagc ccttcatgcg
    10501 gaacccattc gatttatgac ggaagttttt ccggaataaa aaaaaatggt gccggagtgg
    10561 ctactgctta ttttattcag caaaaaatga ctgtgctctc tgatgaagaa tggctgaaaa
    10621 ctagaggtga gcagaatgga aatcgaaaag accaatcgaa tgaacgcgct ctttgaattt
    10681 tattcgacct tattgacaga aaaacaaatg aactacatgg aattatatta tgcagatgat
    10741 ttttcattag gtgagatcgc ggaagaatac gaagtaagcc gacaagcagt atatgacaat
    10801 atcaagcgga caagcaagat cttggaggat tatgaaaaaa aactccatct tttttctaat
    10861 tatattgttc gagaacaggt attggaaaat atgactgcat atattacaga taagtacccg
    10921 gaagataaaa aactacttga atatgtacag caaattcaag caatcgaaga ataaataaaa
    10981 ggaatgaaga aaatatggca tttgaaagct taactgaacg tctccagcag gcgatgggaa
    11041 aaataagaaa aaaaggaaaa gtatcagaag ctgacgtaaa agaaatgatg cgggaaatcc
    11101 gccttgcctt actagaagcc gatgttaact tgcaagtagt caaagatttt actaagcgcg
    11161 tacgcgaacg tgcggtaggg gcagaagtgc tggaaagttt atcacctgca caacaaatcg
    11221 taaaaatcgt tgatgaagaa ttgacgatca cacttggttc tgaaacagca gagttaaata
    11281 aatcaccaaa aataccaact gttatcatga tggctggtct tcaaggggca ggtaaaacga
    11341 cctttactgg taaattagcc aattatttaa agaaaaatga aaatgctcgt ccgctcctaa
    11401 tcgcagggga cgtatatcga ccagctgcga tcgaccaatt gaaagtatta ggtcaacagc
    11461 tagacgttcc tgtatttgat atgggaactg aagtcagccc ggtagagatc gtccgtcaag
    11521 ggatggagtt agcaaaagaa aagaaaaacg actatgtatt gatcgatacg gcaggacgtc
    11581 tgcatatcga tgaaacattg atggatgagt tgaaacaaat caaagaattg acgcaaccaa
    11641 atgagatttt gcttgttgtc gatgccatga ctggtcaaga tgccgtcaac gtagctgata
    11701 gcttcaacca gcaattaggt attacagggg tcgtcatcac taaacttgat ggggatactc
    11761 gaggcggggc agcactatcg atccgctcag tgacaggcgc accaatcaaa ttcatcggtt
    11821 ctggtgaaaa attgactgac ttagaagtat tccacccaga tcgtatggct agccgtatcc
    11881 ttggtatggg ggatatgctg acattgattg aaaaagcgca gcaagactac gacgagaaaa
    11941 aagcggaaga acttgcacag aaaatgcggg aaaattcatt cgatttcaat gactttattg
    12001 aacagcttga tcaagtgatg ggtatgggcc cactggaaga tctaatcaaa atgatcccag
    12061 gaatgaatca agtccctgga atcgaaaacg tcaaggtaga tccaaaagat gttgaacgca
    12121 aaaaagcgat ggtttattct atgacgccag cagaaagagc aaatcctgat ctgttgaacc
    12181 caagccgtcg tcgccgaatt gctgccggtt ctggaaacag tgttgttgaa gtgaatcgaa
    12241 tgatcaaaca attcaaggaa tcaagaaaaa tgatgcagca aatgtccaaa ggggatatga
    12301 acatcccagg tatggaccaa atgtttggtt caggcgtcaa aggaaaacta ggcaaaatgg
    12361 cgatgaatcg aatgatcaag aaaaataaga agaaaaagaa aaaacggaaa taagatgaaa
    12421 aaaacagctt cggtacagac tgaaaatgtc tactgccgga gcttttctat cagcaatttt
    12481 tattctttac taatacaatt actctaaacg aagaggtggc acagatgaga tgtccgtgga
    12541 gtgaacaaac agaaagtatg cggcaatatc atgatactgt ctggggtgtt cctgaatatg
    12601 atgaccaaaa gctgttccga aaattgatgc ttgatattaa tcaagccgga ttgagctggc
    12661 agacgatttt aaataaaagt gaagcattcg atgaagcata tgatcgattc gatatcaaaa
    12721 aagtcgcaaa ttatggagaa acaaaaatca gtgaactaat ggaaaacaaa ggaattattc
    12781 gtaaccgaag aaaaatcgaa gcagccgtcc aaaacgccaa agcagtcttg aaaattcaag
    12841 aagaaatcgg cagtttttca gagtatcttt ggtcgttttc taatggaaaa atcgttgatt
    12901 cttcttatca ttcacaagaa gaagtcccaa caacaagtcc tctttcagat gaattagcaa
    12961 aagatctaaa gaaaagagga tttaaattta tcggaagcac aacaatctat gcttttttag
    13021 aagctgtcgg gatcatcaat gatcaccttg acagttgttt ccgcaaagaa gaggtcgtac
    13081 gtatcggcag aaagggcgaa taaagcatgc gaaaaatcat attttatggt gctataagtt
    13141 tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac actccaacag
    13201 gtaagcaaac tacttatgaa gctttttatg aaacaatcga tacgaccatc atgggcagaa
    13261 aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca gagaaaaaga
    13321 atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc gtaaacgaag
    13381 atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc tgggttgtgg
    13441 gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa tggtatatcc
    13501 agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga acatatgaag
    13561 cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg cattatgtac
    13621 gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc tgtacatagt
    13681 tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa atctagatac
    13741 gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc attttcatca
    13801 attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg cacattcaaa
    13861 aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc acctggtgat
    13921 gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg tcttgagcct
    13981 acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg agtaacgaaa
    14041 acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc aggagcctgt
    14101 tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac aaatacactt
    14161 tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt gtaagaatct
    14221 tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt cctccttcaa
    14281 actatctata ataatcttac aaaagataag taactggagc aaataaaaac acttactgac
    14341 aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta aaatcttcta
    14401 ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta cagggtaaaa
    14461 aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa ataatggcag
    14521 ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt atcgtcgtag
    14581 ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact tacaatcctt
    14641 tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg ttatcaaaag
    14701 gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt atgcaaaaac
    14761 accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt taagcgactt
    14821 agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt tagacgtcgt
    14881 agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata ttggccgtat
    14941 cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg gtgttcggat
    15001 caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt aaataaacgt
    15061 gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga agaaatgatt
    15121 atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa cagccaatga
    15181 gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt tcggttactc
    15241 aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc agtccagaaa
    15301 aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca aataatgaga
    15361 aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg cctctagatg
    15421 taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa ggttattcta
    15481 ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg aatcaagaaa
    15541 cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa acaatccaag
    15601 ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt tctcgaatca
    15661 cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa ggaaactggc
    15721 gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta gctaaagaaa
    15781 tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt aaaattactg
    15841 ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa ccgaaaaatg
    15901 cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt tctatgaaga
    15961 ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca caggaagtaa
    16021 tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa agaaaactag
    16081 aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg atataagttg
    16141 actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg cgaagtccgt
    16201 gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt gctaacacta
    16261 ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa acataaaaat
    16321 tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa atatcgggat
    16381 ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga attctattac
    16441 catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa agtaaaagaa
    16501 atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa agctgatata
    16561 ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg agtggtccgt
    16621 gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt tatttccaag
    16681 aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata aaggattatt
    16741 agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga cagtagatga
    16801 ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct atgataacat
    16861 ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc ttgacccagc
    16921 tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac acttagtatt
    16981 catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga cagatgaagt
    17041 ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca tgatcgatgc
    17101 aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa cggattctta
    17161 ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata aagggatgaa
    17221 agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc aattaaaaga
    17281 atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg aatggacacc
    17341 tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa cagcagatga
    17401 agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt tagtatttta
    17461 tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag ctcataagaa
    17521 tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa caaaagaaca
    17581 attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc acgcaaaagt
    17641 tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca aacgccgcgg
    17701 tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg gcgttgaacg
    17761 tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct acggtaaagt
    17821 acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc gtatcaaaga
    17881 aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc aagggttctt
    17941 ttttcttagc atgtaatttg aacaccctga tccagttttt tctttaatta ttggtagggg
    18001 agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga gcactatgat
    18061 aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta acataaatgg
    18121 agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga gcaagaaaag
    18181 atgaaagttt tagacacatt catgaatgat ggaaaagtaa aacaagtacc aagttcaaca
    18241 aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg agtgacttat
    18301 acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga ttatgtcgaa
    18361 caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg tcgggcatat
    18421 cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa aagttcaagt
    18481 atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta tctataatac
    18541 aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg agcgattcgc
    18601 agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa aatcaattgg
    18661 atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac caaaacgtac
    18721 ctcctaaaat tagattttgg acagatgccc tttaaaaaga taacaagatt tcgttcttga
    18781 aacaatatat atgcttttat tccaatgagg ttgtgaaaac agtattttgt cctgagcgtt
    18841 agaaaattga aaatggggaa tagcaactcg tactttacca ttttttaagt gaatacaaga
    18901 tcagctaatt gaacaccgtt tattcgtgat gagggacaca acactgcagt cgacacattt
    18961 ctcttttatg ttcaaagata acaaatattt aaaacaaaga aaaatagtag ataatcccgt
    19021 cctaactctt ttattcttcc ctcatagctt ccaaatttaa gcaatacttc aattaatcaa
    19081 gttttatctt tttttttcaa taatcgtaaa aaaagagtat tttttattta aaaataaaat
    19141 ctttgtatta aaggacaaga ctaaaataac cttagaataa aaagagaaaa aaagccccaa
    19201 actttctata catcaataaa ataataaaaa ttaaatttag aataacgtgt aaaggattat
    19261 ttgatgattt tttattgaga ggagggatga aaatgattaa gaaaactcaa aataaaatcc
    19321 aacgtatatg tcaaaaacat tttgtttcta gactcaaagc tgtatctaaa agaaaatact
    19381 tacatcccat tatgatttcg atcatcttga tttttacttt cttagggagc ttatcaagta
    19441 tttatgtaaa cgtttataca gaaacgtcaa attttgctaa ccaaacgaca gaggagacaa
    19501 atagtccacc ctattctgaa atacagtcca tagaaagcac caatcagtct agcaacaacg
    19561 agacaacctc aaccacatcc tcgactcaac tgattgatcc gacagaaaaa gaaatcacag
    19621 cagcaaaagc agcaggggag aaagcttatt cagaaacagg tagaagccag acatttagta
    19681 ctgttgcggc cactgcgaat ttcaacggac ataccatacc agatggatat acagtgatat
    19741 atgccaaaga tggagtcatt gttgcggtta acggatcgat gagtgcggat aaaactgcgt
    19801 tacaaggcgt aaccaaagca atcgctttta ttgataaccg tccagatatc cataatctgt
    19861 ttattggctc cacagtaaat ggtactacta tcacattccc aaaaagtatc tatatggggg
    19921 tggctaccgc tggaaatacg atcgattcag ttacacccaa tgcggataaa agttattctt
    19981 ttacaccctc aacatgggga gggcttcctg ccggaggagg tatgagtact ttttcaatcg
    20041 gtatacagtt agcaaatgat attccattca cggggtactc gatcaaaaat gggcagatcg
    20101 ttgatggtaa gggaaatgtt tcaaattttc caacctatca agcagcaacg gttcaggtca
    20161 atgcgaatcg gacaggaact ttttcttata cagtcaatgg tggagcagca aaaatcggaa
    20221 cgtacgggac tacattatct ggaatccatg tgggtgacaa agtggttatc actccaacag
    20281 cagtaacggg ctattctttt gcacaaacaa acggttcttt cacagcccta gaaagtaacg
    20341 tcgatacggt gacctataca ggcaaacaaa caccaatcac tattaagtat caagactatt
    20401 ttggacagat gattgctcct tctaaaataa tgaatcttac ttacggaagt gctgcacaag
    20461 acctgacgac taatacgcct actatcaacg gatatacctt tacagctgtt tcagcgacag
    20521 aaacaaagaa ccaatcagca atgagtgttt tagctagttt agatacaaat ggaaatgtaa
    20581 tcgtaaagga cgcaaatgga aaacagattt ctgaagtcat cctatattat aagacaaatg
    20641 caacagtatc aatcaatgca aatggcagta agtactatga tggtctttct gttgtaccga
    20701 tcgttaaata tgcctttaat gataaaacag aatcaacatc ccttttaaac aatgatctca
    20761 gtattgatca aatcacatgg aatccaactg atttcaaagc gatgaatgaa aagggagttg
    20821 aagtaactgc gcctactgaa attggaacct atacgtcttg gcaattgaca aaaagcggac
    20881 tagctaaact agctgccaga acaaactatt tatttactat cgtgcagaca tctgatgtct
    20941 atacgattaa gcaaatcagc ggtgcggtag agctcagtgg cagtaagaca tatgatggaa
    21001 aagcaggtgt cccgtcaatc catgtcaagc tagcagaggg tgtcactagt tcgcttactc
    21061 cagtacaagt ggcacttagc agtatggatt acacagcaga tgcacaatca gcaaaaaata
    21121 tggttaatgc tggcagctat acaattaaac taacgaatag cggaatagac aaagtcaaag
    21181 cagcaaatcc caagttgtca tttactgata tagccaacac atcaggaact tataccatct
    21241 ataaagctaa cgcagtgatt actgtcgatg acgctgcctt taattatgat gcccaaagtc
    21301 actcgatttc ggttggaaat ctccacgtta ccggtgttgt cttaggcgaa tcattggatt
    21361 ataccctaac agttaacagc cggacagata ttggatcaca aatagtaacg attagtttag
    21421 gcgaaggaag tgtaaacaat aattatacga ttatcgcaac agataccgct aagttgacga
    21481 tcaatccgac tctcttgctt ccttcgacag gtgggatagg tctactgccc tttgtctttt
    21541 taggactgat ctttattttt tctggcttct tttattttat ccatcgtaag aaaaaagcag
    21601 gtgaacagcg atgaaaaaaa caaaaaatgt acgtgtggat atatttgctg ttctcttttt
    21661 tatcctactg gtctttttta taggttctcc gactgtgtca gctagagaat cacctattac
    21721 atttactttg cataatcgaa tgttcgaaag cgataagatg ccaagtatca gtcaaaacga
    21781 cggaataaat atcccggata actttcaagg agtaaaaggg ttgaacggcg ttactttcgc
    21841 tatatatgat gtctcggatg aattttacaa gctgcggtca gaaggaagta gtgtggaaga
    21901 cgcacaaaga aaactagcac aaaaaagtga tagtgaaaaa atactggcag aaaatgtgac
    21961 aaaaacagta gacgaggaag agggcattgc ttcttttagt gcatcggaca aagatgagca
    22021 aggtcgtgac gctgtttacc gatttgcgga aataaagact tcagattaag taaaagaaaa
    22081 atcagcacca tttgtcgttg tgcttcctgt gacagattca tctaatcata aattgacgac
    22141 catccacttg tatccaaaga gcgaacaaaa aatacctgct gcactcacat taacaaaaac
    22201 agtagagaat aagcagacag attttgcaga tggggataaa gttccttatc tcatcaccgt
    22261 aacaattcct gagaacatca acgagatagg aacctatacg atcaaagata cagctgatcc
    22321 tcagttatgg ctagaaccag aaacgatcga ttttttgatt ggtggagaca agattcatac
    22381 atttcacaca caaaaaacaa aacatggttt tgtcttatca gattctggaa aagatcttag
    22441 ttcgtttgct gggaaaaaat taacgatccg ttaccaaatg acattgaaag aaaacagtga
    22501 aaaaacgaca tttgataacg atgtacgttt gacgactgac aatcaaacag tagaaacaca
    22561 tcttgccatc caaacaggag gcaaacaatt tgtcaaagtg gatcggcaaa cgaaacaaaa
    22621 actagcagat gctcaatttc tagtcaaaaa aaatgaccaa tacttagcgc aagaaaacgg
    22681 gataaatcat tgggtagcca aagatgattc aagagcaacg atttttactt ccagtaaaga
    22741 tggaacattt tctgtccatg gattgtcttt tggaagttac gaactagtgg agattaaacc
    22801 gcctaaagga tatatattga gtcaatcaac cattctcttt gaagttgaaa aaggcagtta
    22861 tgatccgctt catcctatac cattggaaat catcaacgaa ccaaaagcaa atccgacgaa
    22921 taatggaaaa acgccagttc agatgcagac aaatggtcca tcaaatgaaa aaaatggagg
    22981 aagctttcca aaaacaagcg aagagatgct tggaggtttc tcaattttag ggttgttgct
    23041 agtcctttca acagggacag cttggtttta taaaaaacaa caaaaaggaa acaataggag
    23101 ggaaataaaa tgaaaaacat caaaacaaaa atatggacgt ttttaggaac tgctatcatg
    23161 ctgcttcctt tcgttctcgg cctaggaact gcagaggttt ctaggccgta tcacctacac
    23221 cagaaaatgt tacggttaat ttgcataaat tgaaattcac ttcagcacca gaaaatcaaa
    23281 taaacaacgg aacggaattg acatttccga atagcgaacc gttgaacggc gttgaattca
    23341 acgtatatga catcactgct acgtactatc caagtaaaga cacagcagtt ccagcagacg
    23401 ctacaccctt cgcaagtgta acgacttctg gtgaaggatt agctaacctg actcttccag
    23461 gtaaatctga tgggaaagat gctgtatacg tctttgtaga aacaccaaag cccggtgtcg
    23521 aaacgtcgcc aaatatcgtg ttaagcttac ccttttataa cccgaattca tcagaccctg
    23581 acaaaccttt agacacgatt cacctatatg ctaaaaatgt catcaaagaa agcaatatca
    23641 aaatccagaa agctgggaac ttttcagggg tagataagtt agcaggggcg caattccaga
    23701 tcaaaaattc agagaatcaa ttcgtgaccg gttttgatga aaacactggt gtcgctctct
    23761 atgatcctgc tgagtcagcg ttgacattca caactgatac tgaaggatcc atcagtgtca
    23821 atggattaat agacggcaat tatactttag ttgaaacaaa agcaccagat ggttatcaat
    23881 taaccaatcc acaaaaagaa actactttta tagtaaaaaa cggaaaattg caacaaacaa
    23941 ccggtatcat ccatggcgca gatgaagcca atcttgggaa tccaaacggt gtagtcaatc
    24001 gggaaaatac aactacggat aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt
    24061 tccacttcat caaacaagat gtgaacacgc aagaaaaatt agccgatgca gaattttcta
    24121 ttctaagagc aaaagatcca actgttaaat taatgaaaaa aatagcaaat cttgcctcag
    24181 gagaataccc atatgcttgg tcagatgaag tcacaggttc tgactgggaa gaagtgaaat
    24241 tgatttctga ttcagaaggg aaattttcaa tcgaagggct gaaacaaggg gcatactgcc
    24301 ttcaagaaac aaaagcacca gaaggctacg cattacctaa gaatgaagct gcgtacactg
    24361 aatttacagt agatgatgat ccaacaactt ctgatgaaac aacgatcgat aaccaaccta
    24421 aaggaatctt gccttcaact ggtggtatgg ggatcatcgt attcatcttg gtaggtactg
    24481 ctttagttgg aggagcagtg atctacttca aaaaacgcca tcaagaaaca gaagcttaaa
    24541 ctattggaaa caaaaatcat acgataaata aacgatagtg attcaaatgg ggagaaaggt
    24601 tgtgacaagt ggtcggtctc ttggtattcg ctcaacaaac ggcaaaatag gaggaactat
    24661 tttgccgttt gttgagcgaa tactcaggtc aaggcgctga tttcacaacc tcttcatata
    24721 catattagaa aataggtgga catgatgaat aaaagcagaa taagaatact ggacatcttc
    24781 atggcaatca ttttagtcgt tgggatcggg atcttttctt atccttttgt agaagattca
    24841 ctgaatgatt ttcttgccca gcaaatgatc atccattatc aaaagcaagc atcgaataag
    24901 aattcagagg aaataaaaaa acagcaggaa aaaatgacaa agaaaaacca acagttggca
    24961 gagcaaaacg tatcaccagg gataggcagt ttcaaccaag cagttgatgc aaaagcatta
    25021 aaagatttac ctagtaatgc cttttttatg gaacatatgc ttggagtcat cgaaatccca
    25081 aaaatcaatg tcagcttacc tatattcgat cagacaacgg aaatattttt gcaaaaagga
    25141 acgtcattat tggaagaaag ttcttatccg actggcggga aaaacaccca tgccgttctt
    25201 tctgggcatc gtggactccc agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa
    25261 ggagaccagt ttttcatcca gatcaatgga aagacattgg cgtatcaagt agaaaaaatc
    25321 caagtggtct tacctgatga agtagattct ttgggtatcc aaaaaggcag agatctcgtg
    25381 actttgctta cctgtactcc ttatatggtc aatacacatc gactgcttgt gacggggcat
    25441 cgtattcctt atcaagcaaa agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa
    25501 aaatggaaat tttttatttg gtttatcggt atactacttg gatcaatcgg cttagtttgg
    25561 ctactcatcg cttatttaga ttctttggcc atcgctaagc gaaactatcc tttatctttt
    25621 tatgtaaaga acacaaacgg acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc
    25681 aacggcaaac actatattac tagagaaaaa gttccatttg tgaaagcttc tgatgaatac
    25741 ggactagtga gattttctga tttaaaaggc aggaactatc gcttgcaaca tgaggagctc
    25801 ctgcttaaaa tccatgtaaa acataaacat tcgaaacagt tttcaatgaa attgaaaaaa
    25861 ggaagatata aactgcgaaa agaaaaagag gtgtactatt tgattgagaa agaatgaatg
    25921 aagatgtcca aaaaagcaga gggaacagac taaatgaaaa aagagagaag ctgacgaaat
    25981 ggacgtgtaa actgctattt ttggtttact atctggaacg ccagcttcta tttttttatg
    26041 tttcagaaga attttattat tatagtataa cttttgaatt agtttccata acatttggag
    26101 caaggggtta aaccacgagc ttgagcttcg cttaaagtgg caggggtata cgtaccattt
    26161 ccacatttat gtgtatggta ttttgaaccg gtcggtgtga catagacagt ttgttcatta
    26221 ttttgctcag gagctgcggc ttgtgcctgc tgagaggcct gttcagctgc tgcttgctgt
    26281 cgcgcctgtt ctgcttgttg ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt
    26341 cgtgcaattt cttcttgctt tttccgttct tcttctgctt cttttgcttt gattgtggaa
    26401 tcaattgcag caagacggct tgtcaactct tgtttgtttt caggtagcgc ttggatagct
    26461 gctgaagcag ttgcatagtt ttcttttgta ggatttgctt cagcttgtga caaagcagat
    26521 tccgcttctg tcactttttt ttctagtgca gctttttccg ctttttgttt ctcttcttct
    26581 tttttctttt caatttgttc ttgttctttc gatgaatctg ccgtactttc ggagatcgtg
    26641 gtactgctgc tggaagcctg agctgaactt gtgctttgag tagtagacgt agtagcacca
    26701 cacccactca acaaacttcc tgcgaccaaa aacaatgcaa ctaatttttt cactaaattt
    26761 cctccttaag taaaaaacaa ccgttttcaa taaatgtcct tttcaattgt ttacttactt
    26821 gttataataa ttgtgttatg aatagtgatt gaggaaactt aatctaatcc cgttttgata
    26881 cacgggattt tttgctatag ttctgttatt aagtatgcaa taataaaata tgtacgttat
    26941 tttattatta gattattgac atttatataa taaacgatta aatgattcat atcaatagaa
    27001 tacttaggaa aaaaagagaa ataataacag gtttatgcac tattgacggt gtgacaatat
    27061 gatatttgta tccgtttttt ttcttgtatg tagtatagta aagtagatac aggaggagaa
    27121 aatatgcaga cttttttaac aaaagtttct caaaagaaaa ttaaaatgat tcatttactg
    27181 cttgaaacag aacgttggtg ttcaatagaa gaactacaaa acgaattgaa ggtatcaagt
    27241 aagtctatct tgaattatct aacagattta gaagaattgt tccaaaaata tcctgataag
    27301 gtcattttga agaatgagaa taatcggcgc ttttctatgg aaaagcagga gaacttccca
    27361 atctacatta tttatcttca tttctacaga aaatcatata attatcattt gattgaattc
    27421 atgtatcaac atcctgagaa aaatttggaa gattatgcag acgctcaata tacgagtgta
    27481 tccacagttt ttcgttatgc taagttatta gtaaagtact ttgaaatgac ggacgtcaaa
    27541 gaatagtagc cgccagatag aaggaaatta gcagttgaaa aagctgccct gcatcaagta
    27601 atacgaccag ccaaacggaa acagtgtctc atgtttttga tttggctggt cttattaccg
    27661 cagatgcaag cttttgccac acctctttct tgtcatttac agactcaact atcaatgctg
    27721 caagctttcc cttaatgctg taacaccagt aagagttgtc aagaccagca gactgactag
    27781 gtaaataccg ctcaaaaaca agattacggc agttaaacta taatgatcca tcaagaaacc
    27841 gatgacgata gaagaaaaac caccaacagc tcgtccaagg ttcataatga aattattcgc
    27901 cgtcgcgcgg atttctgttg gatacaaact actaatcact gcgccatatc caccatacat
    27961 gccattgata aagtagccga ctatcacagc ggctgctacc aaactccact ggctatgggc
    28021 taacacaagt agatagacag ataaggatga acaaattaaa aacaaagaaa aggctagttt
    28081 aggccccaat ttatccatga ttatgccaaa agtcatcatc cccaaagaca tgcctaaaat
    28141 agtactgacc atccacaatg aagacccaga aaccgaaagc cctaatcgat tttgcataat
    28201 tgaaggcagc caattcatta acccaaaata gccggcaatt tggacagtta ccatgatact
    28261 aaggccacat gtttgccaag caagatgttt tgattcaaac agtctgctaa gtttggtttt
    28321 cttttcggca gattgtacag atttgaattc ctttggttca gtcaagccgc gacgaatgaa
    28381 caagaccatc aacaccggca gtacacctac gacatacaac atcttccaac caaacgttgg
    28441 aatgatcaca gcagctaaaa tcgcagccaa agccgcaccg acctgcgcac caataccagc
    28501 aaccgaagaa gctcgtccaa tttgtttttt tgaaaaactt tcggaaatca aggacataca
    28561 cgccccgtat tctcctcccg ctcctacacc tgcgataaag cgaaagagat aaaccaagta
    28621 gatgtttgaa gcaaaataca tcaataaaga agcaatcgaa aacagaagga ccgtttgaga
    28681 aaatactttc acacgtccat atttatcagc tatgatgcca aaaaaaatac ctccaatgag
    28741 catccccaaa ttagtgatcg ttgaaatgaa acccgcttgc gtaccactaa tatgaaaaga
    28801 agtaatcatt gaagataatg aaaaagctaa aaacataata tccatattct ctaaagcaat
    28861 tcccgccgat gtcgataata ataactttcg ctgatacgtt gtcattttta tacccctttt
    28921 cctaaaattt ccctaaattg ttaaaagtat atccaaaaaa gaataaaaaa acaagagttt
    28981 tttatttgaa ataatacaca aacaaccgat atagataatc cagcttagtt ttaatagaaa
    29041 tgagtattga cttggagtac acttaaagtt gtagaataaa taaaatacat aagtaaagtg
    29101 gcaagaaatt aagagaaaga gtgatcagat gaatatcaaa caagtcagtg aagaaaaggg
    29161 catttcggcc gatacattgc gctattatga acgaatcggg ttgattccac cagttaatcg
    29221 gacaaaaggc ggaatccgtg attatacaga agaagaccta aaatgggtcg actttacgat
    29281 ctgtatgcga aatgccggat tatcgatcga gtctttgatt gaatatattc gcctatctag
    29341 cgaaggagaa gcaactgtat ttgagcggcg taagcttttg attgaagaaa gtgaactttt
    29401 gacgaaaaaa attgctgaaa tgcaagaatg tttggaacgt ttacaaggaa aaatcaagaa
    29461 atatgatcgt gtgttatcta gaaataaaat ggaatgtatc aagtaaaaaa atcaaggagc
    29521 atcgctatgg agttacgctt actaaaatat ttttggacaa ttgcagaaga aggaacaatc
    29581 tctcaagccg cagaagttct acatcttaca caaccaacct taagccgaca actgaaggag
    29641 ttagaagagg aactaggaac agaactgttt gtacgcgaaa accgcaaaat gatcttgaca
    29701 gaagcaggtt attttctaaa aaagccgagc agaagaaatt ttagatttaa cgaatcaaac
    29761 cactcaagaa ttcgagaaca gaaaaaaaca attattcagc ggccgattta cgatcggctg
    29821 tgtggaagct gataattcga atacattggc actattacta gaaaagttga tccgtgatta
    29881 tcccagcgtt caatttcata tccacagcgg aacaagtgat gatatcacgg agtgactaga
    29941 aaaaagcctg ttagatttag cgattttgct agatccagta gatacagaga aatacgaaac
    30001 gattttgtta ccaagaaagg aaaaatgggg attgctcatt ttagaaaatt ctgttttagc
    30061 aaaaaaagac gtgataacaa aagaagactt tgcgaatgta cctcttttga tctcaaagcg
    30121 tccagaaata cagcgaatgt ttgctgactg gttaggcata aaaaaggagc agttgctgat
    30181 cacaggaacg tacaatttga ttttcaatgt atttgcgcta attgagaatc aggtaggtgc
    30241 tgctttagtc atagaaggtg ctgtaagtca tctttcaaag cgagtgacat tcgttccgct
    30301 tgacccaatg attgaaacga actgcatgct agtctggaaa aagaatcgga ttttatcacc
    30361 tgtagtcaat gaattgattc accgtttcaa agaagctttt gaacagtaat aagtatctct
    30421 aatcgaagaa atttcaatac aaaagcgaga cgtatgacag tcttgctttt gtattttttc
    30481 atttttaaat caaaaaaaat aaagagattt acataacttg ttaaataagc gcttacaaac
    30541 ataagaagaa cggaaatgaa tcagctataa taaccgttgt tctggattgt aatcattaca
    30601 aagaaatgaa gattataact ttggaggtgt tcgaaatgaa agaaaaaaag caaaaattcg
    30661 ttcaaaaaac atgcccttta ttcaatggtt gggtatgatt ctgctgattg tattagcttt
    30721 attgacgatc attccttttt tatggatggt ttctgcttca ttcaaagcaa ataatgaagt
    30781 gttcacgatt ccgattcaat ggatccctaa aacttggcat ttagagaatt atctggttat
    30841 ttggtctcgt attcctttag tgacattttt taagaataca gcttttttaa gcgttgtgat
    30901 cacgttgatt cagttgttta cttctagttt tgctgcttac gggttttcta aactaaattt
    30961 taaagggcgc gatattttat ttttagcata tatcggcacg attgctgtcc cttggcagtc
    31021 ttatatgatc ccgcagttca ttatgatgag aaaagtgggt ttaacatata cgttgtggtc
    31081 gttggtgcta ttacaagcgt tcaatgcttt tggtgtgttc ttgttgaagc aatactacag
    31141 tagtatccca gattctttat gtgagagtgc gcggatcgat ggcttatcag agtggggaat
    31201 ctatcgcaag atcattttac ctcttacaaa accggcgatt gccagtttga cgatcatcac
    31261 attcgttaat acatggaacg attatatggg gccattcatt tatttgtctt ctacggaaaa
    31321 taaaacaatc cagttagggt tgaaaatgtt tattgggcta tttgatgcag agtacgcatt
    31381 aatcatggct gcctcagttg tttctgttct tcctgttgct tttatatttt tagcgatgca
    31441 aaaatatttt gtagaaggga tcgcttcatc aggaatgaag gaataggagg tgattatgaa
    31501 agagcagcgt tttgacaaaa atgtttatat ggaattcttt tattggttgt atcaatcatt
    31561 ggcaagtagc ctttgttttt ggtgtgtcaa tatcttgtat atagcagctg cgttcctttt
    31621 gtcgatagat ggaaggaatg tcttttcctt tattttttct ttacttttta ttggtccagg
    31681 ttgcatcgtg atgataggta tgacagaaga attagcaaaa gaaaaaagtt tttcaccagt
    31741 acgtttgttc ataaagacgt ttcgccaatt ttggctaaag ggattttttt attggctatt
    31801 tgcttggata gttagtgtga taatgatttt tgattgtttc ttttttatac gtttttctta
    31861 tggaaaatgg ttgattccgt tgtttgtttt acttgcttgt ttatcagttt cctttagcat
    31921 caactactgg tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat
    31981 agcgttctac tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac
    32041 aagtttgttt ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat
    32101 cgtatttggt ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac
    32161 tttataaatt gaaactttca attaagaatg acagttttta tgtaaaaact gtcattcttt
    32221 ttcatttttt gaaacatatg gctaaacaaa tgaaataaaa cgtgctatac ttttgaatga
    32281 aacaggttta gaaactgtat caattatttg tgattatttc taactaataa ttaaaaataa
    32341 agcataaaaa ggacgatgtt caatggacga aaaaatgaaa caaggttcga aatctgtagt
    32401 agaaagtttg attaatcatc atgttgatta tgtattcggt ataccaggtg caaaaatcga
    32461 tggtgtattt aatgaattag aggatcaagg tcctgaatta atcgtgactc gtcacgaaca
    32521 aaatgctgca tttatggcac aagcaatcgg acgtattaca ggagagccag gagtagttat
    32581 cgcaacaagt ggtcctggtg ctagtaactt agctacaggt ttggtaacag cgacagcaga
    32641 aggtgaccca gttttagcaa ttgccggaca agtaaaacga agcgatctat tgaagttgac
    32701 ccatcaaagc atggataatg cagcactgtt ccagccaatc acaaaataca gtgcagaaat
    32761 ccaagatcca gaaacaattt cagaagtgat tgccaatgct tatcgtatgg ctaagagttc
    32821 aaaaaaaggc gcgagcttta tcagtatccc gcaagatgta gtggatgcac ctgtccaagg
    32881 gaatgtcatc aagccattga gcgatcctaa attaggttct gcttctgcag atgatatccg
    32941 ttacctagct gaacgcatta gagaagcaaa actaccggta ctattagtag gtatgcgagg
    33001 atcaagtgaa aaagaaacgt tagccatcag gcaattagtt ggaaagacag cattgcctgt
    33061 tgtagagaca ttccaagcag ctggagtgat ctcacgtgaa ttagaagcgc atttctttgg
    33121 tcgtgtgggt ttatttagaa accaaccagg agatatgctt ttgaaacgaa gtgatctagt
    33181 tattgctatt ggctatgatc caatcgaata tgaagcaaga aactggaatg cggaaaaaga
    33241 tgcgcggatc attgtgatcg atgaagcgcc agctgaaatc gatcctttta tgcagcctga
    33301 aagagaattg atcggtgata tctcagccac tcttgattta ttgacaggtt cattagagcc
    33361 tcaacaagtg tcagaagatg caaaagaata tttagcatct cttcaagcag aattaacgga
    33421 gcgggacatt gttcaatcaa aaggagaagc aggtatcttg catcctttag aagttattaa
    33481 cacgcttcag tcaaaagtaa cagatgatat gactgttacc gtagatgtag gtagtcatta
    33541 tatttggatg gcacgccact ttagaagtta tgaacctcgt catttgttat tcagtaatgg
    33601 tatgcaaacg cttggtgttg cattgccatg ggcgatttct gctgcattgg tgcgtccaaa
    33661 tacacaaatc gtatcagttt ccggcgatgg gggattttta ttctctgccc aggatttaga
    33721 aacggcggta cgtaaaaaat tgaatatcgt tcatttgatt tggaatgatg gacactacaa
    33781 tatggtagag ttccaagaaa aaatgaaata tcagcgggcg tcaggtgtag actttggtcc
    33841 agttgatttt gtgaaatatg cagaagcttt tggcgctaaa ggaatccgag ctacaagtgt
    33901 agaggaatta gaaaaagctt tagaagaagg atttgcgaca gaaggacccg taattattga
    33961 tattccgatt gattaccgtg ataatgagaa attgggagaa acaatcttac cagatcaatt
    34021 ctattaagga agtgtcgaca tgactgaaaa aatcttatac cagcacggga cattaggagc
    34081 tttgatggct ggcttgatgg atggaacaga aacgattgct catatattag aaaaaggaac
    34141 tctgggattg ggaacattgc atgggcttga tggtgaagtc atctttttag atggcgtagc
    34201 ttatcaagga agatcagatg gctcagttgc ccaattagat ggaagtgaac ttacccccta
    34261 tgcagcaatt acagatttta cgccagatac tagtttttct gtctcaaaaa ctgcagatgg
    34321 agaacaattg aaaaaagaca tattagtaag agaagccggg gaaaacctat ttttggcagt
    34381 gaagataact ggattattca aaaatatgca catccgtatc atgccgaagc aagaaaaacc
    34441 ttacagaaga ctagcaaaaa tttcagagtc tcaaccagaa ttccaacaaa gcaatgtcca
    34501 aggaacatta gtcgggttct ttacgcctga attattccaa ggagttgctg cagcgggatt
    34561 tcacctgcat tttatcgatg atacacatac atttggcgga catgtaatgg attttgaagt
    34621 ggctgaagga aaagttgaga ttagccgcat ctcgtccctt gtacagcatt ttccagttga
    34681 tgatccgact tttgttcacg aaacgattga ttatgctgat ttagcagcag aaatccaaca
    34741 agcagaatag aaaaagacag cgagatgcgt tcaaggtgat taacctgacg tatctcgttt
    34801 ttttgaggag aaaacctagc gtttacaaaa aaactgagct ataatcttct ccatagatca
    34861 aaaaggagcg gtttttatca tgtggacaga aaaacagaaa aaagatgcat acgaaatttt
    34921 attattacag caacaaggat tattagaagc agaagataac tggttagcaa atttagcaaa
    34981 ttcatctgca ttgttgaatg agactttgcc tgagactgtc tttgcaggat attatttatt
    35041 cgatggtaat gaattgattt taggaccgtt ccaagggcga gtttcttgca cacgtatcaa
    35101 gatgggaaaa ggagtttgtg gagaatcagc tgaaaaacaa gaaacattga tcgttgatga
    35161 tgtcaaaacc catgcaaatt acatttcctg cgattcagcc gcacaatcag aaatcgtagt
    35221 gccaatggta aaagaaggta atttaatcgg tgttttagat atcgattgcg gagtaacgaa
    35281 agggtatgat gaaatcgatc aagcatttct agaaaaatat gtagatcttt tattagcaaa
    35341 ttttaatata aatgcttttt catttattaa taactaaaat cgtctattga aacaaaaaag
    35401 aagagaaatc aagaataaaa agagccggaa agacagtggg catttctgtt tttcggtttt
    35461 tttatttttt tataaaatca caagtgttat tgattttcat aatcatattt taatgttatt
    35521 atcattttaa ataatgaaaa aacattaagg atggatgaaa tatgaataac gaaactatca
    35581 ttttaggaaa aaaatatatt tgccaaccta ttggcttgaa acaaaaagtg attggagaag
    35641 taatcagtaa attagaaaat tgtgttgtga tctgcgtaga cgaatatcat gacgtcgatc
    35701 gcaatgaaat attagaaaaa tgcggaaaag tagtggcaaa atatactcac gtttatgagt
    35761 tggaaacaac agaatatcta tataactata gcaacaaata tgaacatact ttagtttcat
    35821 aaagatttaa ataaagtgcc ttcaaaagcg agaagaccaa gcttttgaag gcactttatt
    35881 tagttggcag tgattgttca tgatttttga tttattcaaa aggttcgcct agagaatctg
    35941 tctctgtaac gaggcctggt ggagaaactc gataaaaacc tatactgcca gaactgtcct
    36001 attttcggat agacaaacta gtagttttaa aaagatcatc cgatccaatc atttcaaaaa
    36061 atgcaaaact gatatcagga tcatttagct caggcttgtt atcgtaaagc atttgtaaag
    36121 gttgcattta gttaggagta aacgcgaaac tatcatcatt atttgctgaa gaagatgaag
    36181 aagctaattc agagtttgtt tgaaataaag gagacgtgct gacagtttct ttaccctgtt
    36241 ttttgcagag gtagaaactt ttatatcgga agtttctacc ggagaatttt tctcatttgt
    36301 agaaattgtt gaatcgatac tattcgaaat ttgcgtatct gaaggtatgt tctttgatgc
    36361 agaagtccca caagcagtaa gtaacaataa atttgataaa ctgataaaca gaattctttt
    36421 cattatgtac acactcctta tagtatcgtt tagcatacac ctatgctcag tattaggcaa
    36481 aatcaatgca aaactatcaa gtcttttata gtatctatta gtcttagtaa tacatatatg
    36541 gagagggtga tatcccagtt caaaccagat acattggtta ccatctgtat taacgattat
    36601 gcctctaaac gcttttttta tgaaaaaatt tataagtgaa aacgtaggta tgatattatc
    36661 tagaggtaat ttttttgggt atttaaatag ttgctaggag gatagctttt ggatttttac
    36721 agtaatttta tcttaataat agcaatatta ttacttctaa atatttggtt ttttgataag
    36781 agtagaaatg cagggatagg ttttagaacc aaacgaagta cgtcttctga aaaaaagtgg
    36841 gtatattcac agactatttt ttacggaggg gttatttcaa tcagtttact ttcttcaaca
    36901 ctttattctt ttaatgttat agacgtttcc atgtcaaatt ttatatctat tattggaatc
    36961 cttatttctg caattattac acaactactt cttgtgtttg aagagaagtc aaaaaataat
    37021 taaagcaatt atgcctttta gtacaggaat tattacttct tacaaatgta tagcgaatgt
    37081 gaagcctgct gttttaattg ttctagaaaa acttttgagt gaacaattat tttatgttag
    37141 gatgaattaa tttctctaga gttta
//
LOCUS       DAZKSU010000022        37102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000022 DAZKSU010000000
VERSION     DAZKSU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.022"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(160..444)
                     /locus_tag="ACG8Z3_001132"
     CDS             complement(160..444)
                     /locus_tag="ACG8Z3_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823464.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="ACG8Z3_001133"
     CDS             complement(462..1352)
                     /locus_tag="ACG8Z3_001133"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0823465.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="ACG8Z3_001134"
     CDS             complement(1372..1590)
                     /locus_tag="ACG8Z3_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0823466.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="ACG8Z3_001135"
     CDS             complement(1560..3260)
                     /locus_tag="ACG8Z3_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0823467.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="ACG8Z3_001136"
     CDS             3527..4300
                     /locus_tag="ACG8Z3_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823468.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="ACG8Z3_001137"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="ACG8Z3_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0823469.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="ACG8Z3_001138"
     CDS             complement(5124..6095)
                     /locus_tag="ACG8Z3_001138"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0823470.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="ACG8Z3_001139"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="ACG8Z3_001139"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0823471.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="ACG8Z3_001140"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="ACG8Z3_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0823472.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="ACG8Z3_001141"
     CDS             complement(9050..9874)
                     /locus_tag="ACG8Z3_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0823473.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="ACG8Z3_001142"
     CDS             complement(9847..10527)
                     /locus_tag="ACG8Z3_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823474.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="ACG8Z3_001143"
     CDS             complement(10550..11488)
                     /locus_tag="ACG8Z3_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0823475.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12584)
                     /gene="ispE"
                     /locus_tag="ACG8Z3_001144"
     CDS             complement(11733..12584)
                     /gene="ispE"
                     /locus_tag="ACG8Z3_001144"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0823476.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="ACG8Z3_001145"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="ACG8Z3_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0823477.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..16258
                     /gene="proC"
                     /locus_tag="ACG8Z3_001146"
     CDS             15452..16258
                     /gene="proC"
                     /locus_tag="ACG8Z3_001146"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0823478.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16337..17893)
                     /gene="rny"
                     /locus_tag="ACG8Z3_001147"
     CDS             complement(16337..17893)
                     /gene="rny"
                     /locus_tag="ACG8Z3_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0823479.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18304..19353)
                     /gene="recA"
                     /locus_tag="ACG8Z3_001148"
     CDS             complement(18304..19353)
                     /gene="recA"
                     /locus_tag="ACG8Z3_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0823480.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19462..20703)
                     /locus_tag="ACG8Z3_001149"
     CDS             complement(19462..20703)
                     /locus_tag="ACG8Z3_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0823481.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="ACG8Z3_001150"
     CDS             complement(20943..21521)
                     /gene="pgsA"
                     /locus_tag="ACG8Z3_001150"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0823482.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21730..22653)
                     /locus_tag="ACG8Z3_001151"
     CDS             complement(21730..22653)
                     /locus_tag="ACG8Z3_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0823483.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(23392..24678)
                     /locus_tag="ACG8Z3_001152"
     CDS             complement(23392..24678)
                     /locus_tag="ACG8Z3_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0823484.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24671..25939)
                     /locus_tag="ACG8Z3_001153"
     CDS             complement(24671..25939)
                     /locus_tag="ACG8Z3_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0823485.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26191..26652
                     /gene="mscL"
                     /locus_tag="ACG8Z3_001154"
     CDS             26191..26652
                     /gene="mscL"
                     /locus_tag="ACG8Z3_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0823486.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26711..27157)
                     /locus_tag="ACG8Z3_001155"
     CDS             complement(26711..27157)
                     /locus_tag="ACG8Z3_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0823487.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27250..27495)
                     /locus_tag="ACG8Z3_001156"
     CDS             complement(27250..27495)
                     /locus_tag="ACG8Z3_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0823488.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27610..28188)
                     /locus_tag="ACG8Z3_001157"
     CDS             complement(27610..28188)
                     /locus_tag="ACG8Z3_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0823489.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28265..28795)
                     /locus_tag="ACG8Z3_001158"
     CDS             complement(28265..28795)
                     /locus_tag="ACG8Z3_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0823490.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="ACG8Z3_001159"
     CDS             complement(28799..29659)
                     /gene="rlmB"
                     /locus_tag="ACG8Z3_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0823491.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29652..30047)
                     /locus_tag="ACG8Z3_001160"
     CDS             complement(29652..30047)
                     /locus_tag="ACG8Z3_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0823492.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30044..31456)
                     /gene="cysS"
                     /locus_tag="ACG8Z3_001161"
     CDS             complement(30044..31456)
                     /gene="cysS"
                     /locus_tag="ACG8Z3_001161"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0823493.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31453..31989)
                     /gene="epsC"
                     /locus_tag="ACG8Z3_001162"
     CDS             complement(31453..31989)
                     /gene="epsC"
                     /locus_tag="ACG8Z3_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0823494.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(32075..32314)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(32311..33771)
                     /gene="gltX"
                     /locus_tag="ACG8Z3_001163"
     CDS             complement(32311..33771)
                     /gene="gltX"
                     /locus_tag="ACG8Z3_001163"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0823495.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMDWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33941..35098)
                     /locus_tag="ACG8Z3_001164"
     CDS             complement(33941..35098)
                     /locus_tag="ACG8Z3_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0823496.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35132..36502)
                     /gene="radA"
                     /locus_tag="ACG8Z3_001165"
     CDS             complement(35132..36502)
                     /gene="radA"
                     /locus_tag="ACG8Z3_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0823497.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36565..37092)
                     /locus_tag="ACG8Z3_001166"
     CDS             complement(36565..37092)
                     /locus_tag="ACG8Z3_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0823498.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        10250 a         8090 c         6505 g        12257 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg
    16261 atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct
    16321 tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta
    16381 ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta
    16441 aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc
    16501 cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac
    16561 ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg
    16621 agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat
    16681 gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg
    16741 caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga
    16801 gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag
    16861 ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc
    16921 gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg
    16981 ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca
    17041 tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc
    17101 gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat
    17161 cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc
    17221 caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg
    17281 aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat
    17341 ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca
    17401 gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa
    17461 ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt
    17521 caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt
    17581 tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt
    17641 gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct
    17701 gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg
    17761 cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg
    17821 actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa
    17881 tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca tctatacagc
    17941 ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa
    18001 acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga
    18061 aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata
    18121 taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat
    18181 atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa
    18241 aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa
    18301 tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac
    18361 ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg
    18421 atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata
    18481 agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc
    18541 tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg
    18601 tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg
    18661 tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg
    18721 tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac ggatttggtt
    18781 gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg
    18841 agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc
    18901 tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac
    18961 taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc
    19021 atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat
    19081 gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt
    19141 tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac
    19201 acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat
    19261 tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc
    19321 agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa
    19381 tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa
    19441 ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc
    19501 ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga
    19561 aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa
    19621 cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg
    19681 tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag
    19741 ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc
    19801 tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt
    19861 caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt
    19921 tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt
    19981 ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag
    20041 tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc
    20101 aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac
    20161 tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc
    20221 cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc
    20281 tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc
    20341 gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata
    20401 tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc
    20461 agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag
    20521 ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac
    20581 aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc
    20641 attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt
    20701 cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa
    20761 gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt
    20821 ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt
    20881 gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat
    20941 acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag
    21001 atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg
    21061 aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact
    21121 tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct
    21181 gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg
    21241 tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac
    21301 ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg
    21361 atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct
    21421 cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc
    21481 atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc
    21541 cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat
    21601 ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg
    21661 acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa
    21721 tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt
    21781 ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc
    21841 accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt
    21901 tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta accatacacg
    21961 attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat
    22021 ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt
    22081 tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc
    22141 ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg
    22201 acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa
    22261 taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc
    22321 ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt
    22381 gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta
    22441 ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc
    22501 gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat
    22561 ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc
    22621 atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt
    22681 ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc
    22741 tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac
    22801 ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc
    22861 aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg
    22921 tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt
    22981 tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt
    23041 ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg
    23101 gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa
    23161 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt
    23221 acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt
    23281 tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc
    23341 ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg
    23401 tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac
    23461 gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa
    23521 taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg
    23581 tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc
    23641 gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc
    23701 gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc
    23761 gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg
    23821 gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt
    23881 gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact
    23941 ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg
    24001 gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt
    24061 accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa
    24121 gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg
    24181 gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc
    24241 atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa
    24301 gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt
    24361 agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt
    24421 cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg
    24481 ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc
    24541 gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa
    24601 caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc
    24661 atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga
    24721 tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt
    24781 cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg
    24841 cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca
    24901 gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa
    24961 gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg
    25021 tatccacgct actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa
    25081 gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg
    25141 gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct
    25201 cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat
    25261 aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat
    25321 caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg
    25381 acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct
    25441 tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt
    25501 tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca
    25561 actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta
    25621 ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg
    25681 cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa
    25741 gctctgcaag ttgcgcactt aactttgtct gatccggata attcaaacta tttgtttcca
    25801 acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac
    25861 ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt
    25921 ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct
    25981 tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc
    26041 aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc
    26101 aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact
    26161 aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg
    26221 cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc
    26281 gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc
    26341 acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc
    26401 gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt
    26461 gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag
    26521 caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt
    26581 cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat
    26641 acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt
    26701 ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt
    26761 ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt
    26821 cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt
    26881 cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc
    26941 caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag
    27001 tatcttgaaa aaacgtatgc caagcttgtc catcaatgtc atcaacttct tgataaggac
    27061 actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg
    27121 ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt
    27181 tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc
    27241 gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc
    27301 tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa
    27361 tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt
    27421 ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc
    27481 taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt
    27541 tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat
    27601 ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga gttttactct
    27661 cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa
    27721 atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc
    27781 gcaattttcg attgcttctt ttaataaaag atgctgaatc gctaaaggtt cttgcaatgt
    27841 gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc
    27901 tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct
    27961 tataatgctt ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa
    28021 tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa
    28081 ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa
    28141 tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct
    28201 ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga
    28261 tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct
    28321 cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg
    28381 ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg
    28441 ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg
    28501 tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt
    28561 ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta
    28621 gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac
    28681 gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt
    28741 ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct
    28801 actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat
    28861 ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca
    28921 atcctggact cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat
    28981 tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt
    29041 tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg
    29101 ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga
    29161 tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat
    29221 cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa
    29281 tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca
    29341 cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt
    29401 gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa
    29461 agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga
    29521 aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct
    29581 ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt
    29641 ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct
    29701 tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt
    29761 gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt
    29821 cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga
    29881 atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg
    29941 ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct
    30001 ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc
    30061 atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa
    30121 tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt
    30181 gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc
    30241 attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta
    30301 gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa
    30361 aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat
    30421 ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg
    30481 tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga
    30541 tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt
    30601 tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt
    30661 cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg
    30721 cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat
    30781 ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac
    30841 cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc
    30901 ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg
    30961 ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac
    31021 gggtacggta atacacatca cctgaagatt cataagcata gcctttttca accaacactt
    31081 gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggct
    31141 cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg
    31201 gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat
    31261 tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag
    31321 caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat
    31381 acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat
    31441 tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc
    31501 tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc
    31561 gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat
    31621 ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt
    31681 tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc
    31741 cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc
    31801 agtcaggaaa cgccagaatt gagcattgat cttcgccaac aaaaagagcc ggtgctgata
    31861 taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat
    31921 ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa
    31981 ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata
    32041 aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc
    32101 acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct
    32161 actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag
    32221 aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat
    32281 cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa
    32341 tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc
    32401 tgatacagct acacggatcg gcatgaaaag atttttccct tttacgccag tttctttttg
    32461 aacagctttg atggcagctt ttacagttgg agcatctact acttccattt gttctagctg
    32521 tgctttaaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc
    32581 ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc
    32641 atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc
    32701 atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa
    32761 tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc
    32821 gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg
    32881 ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat
    32941 tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc
    33001 acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt
    33061 tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg
    33121 gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa
    33181 attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga tattatcgga
    33241 ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac
    33301 acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt
    33361 caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg
    33421 ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag
    33481 ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc
    33541 tccaggattt tcaggagatt catcccaatc cattcccaac caagcaaggt tttccaactg
    33601 gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa
    33661 gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat
    33721 atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct
    33781 cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat
    33841 atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc
    33901 aactggttga atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct
    33961 ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga
    34021 tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca
    34081 tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atctaggtag gctactcctt
    34141 gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga
    34201 ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag
    34261 aaactttatt caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt
    34321 tgattaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag
    34381 atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac
    34441 gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga
    34501 tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga
    34561 tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca
    34621 gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa
    34681 ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga
    34741 tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg
    34801 tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat
    34861 aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt
    34921 tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg
    34981 tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg
    35041 aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg
    35101 tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag
    35161 ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt
    35221 ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa
    35281 tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg
    35341 gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg
    35401 ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa
    35461 gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac
    35521 ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta
    35581 ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta
    35641 ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca
    35701 tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc
    35761 gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca
    35821 ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc
    35881 catttgtttt agcgattttg agtagttctg cagtggtttc tcttacttgg ctgacacttc
    35941 ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat
    36001 caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat
    36061 aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt
    36121 cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta
    36181 acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca
    36241 ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt
    36301 ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc
    36361 gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc
    36421 aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt
    36481 gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa
    36541 tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg
    36601 agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg
    36661 ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg
    36721 gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa
    36781 cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc
    36841 tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc
    36901 tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt
    36961 ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg
    37021 tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc
    37081 tctttttctc accggttttc ct
//
LOCUS       DAZKSU010000023        35388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000023 DAZKSU010000000
VERSION     DAZKSU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.023"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..1529)
                     /gene="zwf"
                     /locus_tag="ACG8Z3_001167"
     CDS             complement(3..1529)
                     /gene="zwf"
                     /locus_tag="ACG8Z3_001167"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0823499.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(1544..2191)
                     /locus_tag="ACG8Z3_001168"
     CDS             complement(1544..2191)
                     /locus_tag="ACG8Z3_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0823500.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(2375..2704)
                     /locus_tag="ACG8Z3_001169"
     CDS             complement(2375..2704)
                     /locus_tag="ACG8Z3_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0823501.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            2881..3609
                     /gene="sfsA"
                     /locus_tag="ACG8Z3_001170"
     CDS             2881..3609
                     /gene="sfsA"
                     /locus_tag="ACG8Z3_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0823502.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            3625..4638
                     /locus_tag="ACG8Z3_001171"
     CDS             3625..4638
                     /locus_tag="ACG8Z3_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0823503.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            4638..5915
                     /locus_tag="ACG8Z3_001172"
     CDS             4638..5915
                     /locus_tag="ACG8Z3_001172"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0823504.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            5978..8680
                     /locus_tag="ACG8Z3_001173"
     CDS             5978..8680
                     /locus_tag="ACG8Z3_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0823505.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            8832..9149
                     /locus_tag="ACG8Z3_001174"
     CDS             8832..9149
                     /locus_tag="ACG8Z3_001174"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0823506.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            9179..9499
                     /locus_tag="ACG8Z3_001175"
     CDS             9179..9499
                     /locus_tag="ACG8Z3_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0823507.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            9607..11067
                     /locus_tag="ACG8Z3_001176"
     CDS             9607..11067
                     /locus_tag="ACG8Z3_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0823508.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            11135..11356
                     /locus_tag="ACG8Z3_001177"
     CDS             11135..11356
                     /locus_tag="ACG8Z3_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823509.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            11387..11569
                     /locus_tag="ACG8Z3_001178"
     CDS             11387..11569
                     /locus_tag="ACG8Z3_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0823510.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            11569..11982
                     /locus_tag="ACG8Z3_001179"
     CDS             11569..11982
                     /locus_tag="ACG8Z3_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0823511.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            12105..13286
                     /locus_tag="ACG8Z3_001180"
     CDS             12105..13286
                     /locus_tag="ACG8Z3_001180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0823512.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(13357..13521)
                     /locus_tag="ACG8Z3_001181"
     CDS             complement(13357..13521)
                     /locus_tag="ACG8Z3_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823513.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            13675..13748
                     /locus_tag="ACG8Z3_001182"
     tRNA            13675..13748
                     /locus_tag="ACG8Z3_001182"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13709..13711,aa:Arg,seq:cct)
     gene            complement(13794..13907)
                     /locus_tag="ACG8Z3_001183"
                     /pseudo
     CDS             complement(13794..13907)
                     /locus_tag="ACG8Z3_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(14290..15522)
                     /locus_tag="ACG8Z3_001184"
     CDS             complement(14290..15522)
                     /locus_tag="ACG8Z3_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0823514.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            15776..16345
                     /locus_tag="ACG8Z3_001185"
     CDS             15776..16345
                     /locus_tag="ACG8Z3_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0823515.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(16523..16963)
                     /locus_tag="ACG8Z3_001186"
     CDS             complement(16523..16963)
                     /locus_tag="ACG8Z3_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0823516.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            17121..17885
                     /gene="map"
                     /locus_tag="ACG8Z3_001187"
     CDS             17121..17885
                     /gene="map"
                     /locus_tag="ACG8Z3_001187"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0823517.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            17917..18840
                     /locus_tag="ACG8Z3_001188"
     CDS             17917..18840
                     /locus_tag="ACG8Z3_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0823518.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            18916..20055
                     /locus_tag="ACG8Z3_001189"
     CDS             18916..20055
                     /locus_tag="ACG8Z3_001189"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0823519.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            20048..20848
                     /locus_tag="ACG8Z3_001190"
     CDS             20048..20848
                     /locus_tag="ACG8Z3_001190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0823520.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            20848..21675
                     /locus_tag="ACG8Z3_001191"
     CDS             20848..21675
                     /locus_tag="ACG8Z3_001191"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0823521.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            21680..22387
                     /locus_tag="ACG8Z3_001192"
     CDS             21680..22387
                     /locus_tag="ACG8Z3_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0823522.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            22487..23353
                     /gene="rfbA"
                     /locus_tag="ACG8Z3_001193"
     CDS             22487..23353
                     /gene="rfbA"
                     /locus_tag="ACG8Z3_001193"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0823523.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            23367..23939
                     /gene="rfbC"
                     /locus_tag="ACG8Z3_001194"
     CDS             23367..23939
                     /gene="rfbC"
                     /locus_tag="ACG8Z3_001194"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0823524.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            23961..24989
                     /gene="rfbB"
                     /locus_tag="ACG8Z3_001195"
     CDS             23961..24989
                     /gene="rfbB"
                     /locus_tag="ACG8Z3_001195"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0823525.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            25087..25938
                     /gene="rfbD"
                     /locus_tag="ACG8Z3_001196"
     CDS             25087..25938
                     /gene="rfbD"
                     /locus_tag="ACG8Z3_001196"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0823526.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            25973..28006
                     /locus_tag="ACG8Z3_001197"
     CDS             25973..28006
                     /locus_tag="ACG8Z3_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823527.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            28050..29330
                     /locus_tag="ACG8Z3_001198"
     CDS             28050..29330
                     /locus_tag="ACG8Z3_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0823528.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            29540..30346
                     /locus_tag="ACG8Z3_001199"
     CDS             29540..30346
                     /locus_tag="ACG8Z3_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0823529.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            30358..31578
                     /locus_tag="ACG8Z3_001200"
     CDS             30358..31578
                     /locus_tag="ACG8Z3_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823530.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            31568..33154
                     /locus_tag="ACG8Z3_001201"
     CDS             31568..33154
                     /locus_tag="ACG8Z3_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0823531.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            33193..35331
                     /locus_tag="ACG8Z3_001202"
     CDS             33193..35331
                     /locus_tag="ACG8Z3_001202"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0823532.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT        11212 a         6066 c         7356 g        10754 t
ORIGIN      
        1 tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg
       61 aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag
      121 ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa
      181 gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat
      241 agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc
      301 aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa
      361 aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca
      421 catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg
      481 atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac
      541 caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca
      601 tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca
      661 tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc
      721 aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt
      781 gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta
      841 tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata
      901 ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct
      961 gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct
     1021 gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac
     1081 ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa
     1141 tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg
     1201 tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt
     1261 gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca
     1321 tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc
     1381 cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa
     1441 aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc
     1501 gtaaacaata catttttttc attagacatg tggatacact cctttatatt ttatcgacaa
     1561 aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga
     1621 tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa
     1681 tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg
     1741 taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt
     1801 taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat
     1861 ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc
     1921 agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac
     1981 tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta
     2041 caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta
     2101 gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga
     2161 ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc
     2221 atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa
     2281 ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg
     2341 tttataaagg atatatatat acattgggag ggatctattt ttcagataac aacttatatg
     2401 aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac
     2461 catactgatc acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag
     2521 actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc
     2581 tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt
     2641 ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga
     2701 tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt
     2761 tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt
     2821 tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat
     2881 ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca
     2941 cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga
     3001 aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg
     3061 aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt
     3121 caattgccta atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga
     3181 ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat
     3241 atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt
     3301 gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata
     3361 ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag
     3421 ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg
     3481 gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca
     3541 gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct
     3601 atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt
     3661 actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat
     3721 tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc
     3781 gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct
     3841 ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata
     3901 gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat
     3961 gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag
     4021 attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa
     4081 tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat
     4141 ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg
     4201 tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt
     4261 cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc
     4321 ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga
     4381 gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg
     4441 gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg
     4501 gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga
     4561 aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat
     4621 aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa
     4681 aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac
     4741 taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt
     4801 atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg
     4861 cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca
     4921 aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt
     4981 tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag
     5041 caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat
     5101 ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc
     5161 agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg
     5221 ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac
     5281 attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata
     5341 tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag
     5401 aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac
     5461 tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag
     5521 atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc
     5581 cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag
     5641 gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc
     5701 catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc
     5761 caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa
     5821 aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa
     5881 aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga
     5941 aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat
     6001 cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt
     6061 aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat
     6121 tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga
     6181 tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga
     6241 ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt
     6301 gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta
     6361 tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg
     6421 tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg
     6481 ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat
     6541 attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt
     6601 cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt
     6661 tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg
     6721 gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga
     6781 aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga
     6841 aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa
     6901 acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa
     6961 aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat
     7021 caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt
     7081 tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta
     7141 taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct
     7201 agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac
     7261 taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt
     7321 gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt
     7381 tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc
     7441 agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc
     7501 aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag
     7561 tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt
     7621 tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact
     7681 attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat
     7741 gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact
     7801 cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg
     7861 cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat
     7921 ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc
     7981 gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt
     8041 aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc
     8101 agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt
     8161 caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt
     8221 tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa
     8281 actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc
     8341 acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa
     8401 gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg
     8461 aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca
     8521 atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc
     8581 aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt
     8641 tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt
     8701 acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt
     8761 tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag
     8821 gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga
     8881 gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt
     8941 ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat
     9001 tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag
     9061 gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc
     9121 ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt
     9181 ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa
     9241 gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag
     9301 gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt
     9361 gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga
     9421 tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg
     9481 tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa
     9541 cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag
     9601 taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa
     9661 atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac actgcccgca
     9721 acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag
     9781 tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat
     9841 gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc
     9901 tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg
     9961 gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca
    10021 gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt
    10081 gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat
    10141 ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt
    10201 tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta
    10261 tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc
    10321 aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat
    10381 attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta
    10441 tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc accagtgcta
    10501 gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac
    10561 ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg
    10621 gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta
    10681 ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc
    10741 attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg
    10801 aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg
    10861 acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg
    10921 ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta
    10981 gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg
    11041 gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc
    11101 gttcctcata atgaattttc gaggaggaac tgaaatgatt acaattgcca gtgcaatgca
    11161 acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt
    11221 taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc
    11281 ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca
    11341 atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg
    11401 tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca
    11461 aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt
    11521 cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg
    11581 aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc
    11641 gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac
    11701 gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa ttgaagaagt cattgaaaac
    11761 cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc
    11821 aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc
    11881 atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga
    11941 cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac
    12001 tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt
    12061 tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa
    12121 gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg
    12181 gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt
    12241 acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag
    12301 tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag
    12361 aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg
    12421 atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg
    12481 gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta
    12541 tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca
    12601 gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt
    12661 ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata
    12721 atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg
    12781 agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca
    12841 atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc
    12901 tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag
    12961 atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac
    13021 aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa
    13081 atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta
    13141 tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag
    13201 aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac
    13261 agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca
    13321 cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt
    13381 aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc
    13441 ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt
    13501 ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag
    13561 tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta
    13621 gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct
    13681 gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt
    13741 caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt tgtataagta
    13801 gacattgtag tttctaaact gctatgtcct aaacgtttcg atacgctaag tatatttact
    13861 ccttgataaa gtaaaataga tgcatgcgta tgtcttagtc catcaataaa cagtcgaaga
    13921 gaaaataggg aaaaattgat tactaaagaa aaacttacta ggtcgacggt gtagttgatt
    13981 ttcatacttc aatagattcg aattaaacga tttcgattgg cttataccag tcctatccaa
    14041 cgaaagatat atcgttctta actctagcgg tataacttga agctttacgt aaagccaatg
    14101 ggaatgaact ggagatgact ttttattatt tttagtaata gctgatgggt taatacttac
    14161 aactttcctt ttcaaagtac catataaaaa aacggtgggg ttgtttagcg aaataaagga
    14221 tctaagccaa tagcttagat cctttattct atgctttata ttattttttg catgtgtgcg
    14281 aaagtatact caagcccaat cgccatttcg gaaaatcggt actgtagagc catcttcacg
    14341 gattccgtcg atatccatct tgtcagatcc taccatgaaa tctacgtggg tttgacttct
    14401 attcaatcca gcttctgcta attcttcatc ggacatttct gtaccgcctt tcacactgaa
    14461 ggcataagca gaacctagtg ctaagtgatt cgatgcgttt tcatcaaata atgtattgaa
    14521 aaagatgatg ccagattgag aaataggtga agggtcaggt acaagtgcga cttctcctaa
    14581 acgacgcgca ccttcatctg tatcaagcag ttttgctaag acatcttctc cttgttcggc
    14641 agaaaaatca acaacttttc catctttaaa agtaaatttc atacccgaga tgatggtgcc
    14701 agcataactc agtggctttg tacttgaaat gtaaccatct actcgatgac tgtcaggtgc
    14761 tgtgaacact tcttccgtcg gcatatttgc catgaatttt tctccacgag cattatagct
    14821 tccagcgcct tcccaaagat ggtttttagg caatccgata atgatatctg taccgggagc
    14881 agtatagtga agggcagaaa attgttctcg attcaattct tctgcttttt ttgctaattt
    14941 ttcatcatgc ttcttccatg ctaagacagg gtcttcttca tagacacgag tcgttttgaa
    15001 aatctgatcc cataatgcct caacttgttt ttcttctggc agttctggga agacttttgc
    15061 tgcccattgt ttaccagccg cagccacgac agtccaactg actttatttg cttgagttgc
    15121 cttgcgtaaa ttcattagtg ctttgccatt cgctgattga taagacgcta ctcgatggct
    15181 atctacacca gcaaatgcat cgggatcggc agaaacaacg ctgatcctgc ttgctccttt
    15241 ttcaagccat tcatccgctt gatcgatttt agattgtggt acattctcaa tacgatccgt
    15301 tgcagcatgt aaaagaaatt ctctttggat ctggtcgtca gtccattgaa cgataacctc
    15361 agcggctcct agtttataag cttcttgtgt gatcaatcga gcaaggggtg cctgttccac
    15421 gctgatctgt aaaacaactg tgtgtccttt ttctgtggct acgccagttt ctgcaattaa
    15481 gcgtgcatat ttttttagaa gttcattaaa atcagataac ataataaaaa ttcctcctca
    15541 ccattttttc tggtaactat tagcatgata ccatttaatt atgattcaaa caatgaggct
    15601 ttaaataaaa aaaggaggtt atttttttaa ggttaagttt catgatatat gttactactt
    15661 gctatttcag cttgtgaaga aaatcaaaaa ctaattttgc atatcaaaaa aacatgttac
    15721 aatgaaatta atcaatggtg gaagcgttat aaaaaaatta aggagattaa aaaatatggc
    15781 aagaaaaaag acgattacta gagaacagat tttggcagct gcttatgaag tagctgccac
    15841 cgatggattt acacgattta ctgcgagaaa catcgcgaac aaaatgaatt gttccacgca
    15901 accgatttat cttgagttta aaaatatgga tgacttgaga caagctcttt ttgagcagat
    15961 ctatgatcat ttacaatatg aagtgttcac tgttgagcat acagggaata cgattatcga
    16021 tcttgctttt aattatatcc attttgcaag aagagagaag aagttgtatc gttcactgta
    16081 cttagaagaa tatggtggag gaaaagagat gcagaatttc tcttatactt actttgtgaa
    16141 tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa gagcagatcg aatctttgta
    16201 taatgggaca tggatcatag ctactgggat cgcagctttg atgtcttcaa gcatcatcca
    16261 tccaactgat gaacagattg aaaagatgat ccaagacagt atagatgcta tcttgaaatt
    16321 caaagaacca atcgaaatcg attgaatata ctcaaaagtt ccacaaatca gtaggtaaca
    16381 agagatgtgt caaaataagg aaaagttcgt ttttcttaca gcgtatgcat ttttaactcc
    16441 cgaagaactc ttccatgaat gcaatgaata aaagagccat acagattgaa taaatctgga
    16501 tggctctttt attgcaagta tattaaattt ttgcgactag ttctttagcg aaagcttcta
    16561 aattttcgat atcttcttct tcagcattca gatccacttt aacattttcg gctccttttg
    16621 atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac cgatttgcag aattcatcgt
    16681 aaaaagtatc accagatccg cagacaccgt aaatttttcc caatagatcc aattcttgca
    16741 agtcttcata aaagtctacg atctcatcag gcagatcgcc atcatcgtat gtataagtag
    16801 ctactacgca aatatctgcc tcctcgaatt catctgcatc tacttgggta cattcgttga
    16861 tttcgacttc gatatccaga ttttcaaatg cttctgcaac aatatcggcg atttcttcag
    16921 tgtttcctgt catgctagca tatacgattt ttgctaaagc catgatgtgc ctcctcgttt
    16981 ataattattt aacaattatg aattatacca aaacatcatt gaaaagaaaa taaaacctaa
    17041 agacacctgg ggaatgtcat ttggctaaat gtatgccgct atgttaaaat gagagggatt
    17101 tgtaaaaagg agacgaaaat atgattactt taaaatcagc acgtgaaatt gaaatgatgg
    17161 cagaatctgg agctttatta gcagatgtac ataaaaatct gcgtgatttt ataaaaccag
    17221 gaatcacaag ttgggatatt gaggtatttg tcagaaacta tatcgagagt catggcggaa
    17281 ttgctgcgca aattggcttt gaagggtatg aatatgcgac ttgtatcagt ataaatgatg
    17341 aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa cggtgatttg gtaaaagtcg
    17401 atatgtgtat cgatttgaaa ggcggcattt ctgattcttg ttgggcctat gtagttggag
    17461 aatctacacc tgagatcgac cgattgatgg aagtaacgaa gaaagctttg tacatcggta
    17521 ttgagcaagc acaagtaggg aatcggattg gtgacatcgg tcatgctatc caaacttatg
    17581 tagaaggcga aaatctagcg atcgttcgtg attttattgg tcatggcgtt ggtcctacga
    17641 ttcatgaaga accagcagtt cctcattacg gagaagctgg aaaagggttg cggttgaaag
    17701 aaggcatggt tatcacgatc gaacctatgg ttaataccgg aacttggaaa atgaaaatgg
    17761 atcctaacgg ctggactgcg tatacaagag acggcggact ttcttgccaa tatgaacata
    17821 cattggctat tactaaagat ggtccaagaa ttttgacatc acaaggagaa gaaggcactt
    17881 attgatctat ccagtcaata ggtacgggag aggtacatga aagtttggaa caaaataaaa
    17941 caaaatgaaa atctgatgcg ctttattgag acgactcaaa agcgcatcac tgattcagaa
    18001 atgagtacga cctctgtcgt cgttgcttat tatttactct tgtctttatt tccattgatt
    18061 attgcttttg gtaatatttt accgtttctc cacatcgatc aagagactgt actgacctat
    18121 ttacgagaag tgattcctga gaccgtttac cagtttatcg gtcctgcaat caaagacctt
    18181 ttgacacaaa gttcaggagg cttgttatcc gtttctgcat tagcggcttt atggtctgca
    18241 agtcaaagta tcaatgcttt gcaaattgcg atgaataaag cgtatggcgt agaaaataga
    18301 aaaaattttt tgatcgtccg tttcttctca ctaattgtca ttgtcttgtt catgattgct
    18361 attagtgggg taactatcgt tcttgggttg ggacagctga ttttggatgc tattcagcca
    18421 atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa ctttgaagtg gccaatcaca
    18481 ttggtagctt tatttgtcat catgttcttg atttatttaa ttgtccctaa tgctcagttg
    18541 aagttgaaga cgatcattcc gggcacgatc ttcgctactg tcggctggat gctgttatca
    18601 caagtgttta gtatctatgc taggtatttt gcaacgagga tcagcggtta tcagatcata
    18661 ggaagtttta ttgtactcat gctttggctt aattttgctg ctacgatcat cattttgggc
    18721 ggaatcatca atgcagttgt tcaagaatac gtgacgggtc atcaaatcaa aaaacgtcga
    18781 gatccaagcc gaaaatggtt caataaattg aaaactaaat tttctcataa aaaaaagtag
    18841 aaacaagagt tcttattgta aatttatatc agtaatttat aataattatg actcttaatt
    18901 aaagtaggtg cccatatgct tgtttctttt agtatggtta taagattttt tctgacaatt
    18961 ttattatcac tgatattaac acctgtattc aaaatcattt cagtacaaac aggtatggtc
    19021 gacaaaccaa atgaaagaag aataaataaa gttccaatgc cttcagcggg cggactgcct
    19081 atctttattt catttgttat atcgacttta ttcttattcc gtaaaattat ccctcaatcg
    19141 tatattgtac cgatcttgat tgcttcttta gtaatcatag tcacaggagt gctcgatgat
    19201 aaatatgagc tgacacctaa acaaaaaagt atagggatct tgattgcttc tcttattatt
    19261 tattttgtag cagatatacg tattgattcc ttcacgcttc cttttattgg atatatccaa
    19321 ctaggctggc ttagttttcc cgtaacgatt ttttggatct tcggtatcac gaatgcggtc
    19381 aatctgattg acggtttgga tgggctggcg gcagggattt cattgatcgg tttaattacg
    19441 ataggaatca ttggatattt tttcctccat gcttctacag tgtatattcc agttgttatt
    19501 ttctgtcttg tagcaagtat tatcggtttt tttccttaca atttttatcc ggcaaagatt
    19561 tatttaggcg atacaggagc tctattccta gggtttatga tggccgtcct ttcactacaa
    19621 gggctgaaga acgtgacttt tgtttcatcg atttctttgc tgatcgtcat gggagtgccg
    19681 gtaacagaca cgttttttgc tatcattagg agaaaagcaa accgtgtttc tttttctaca
    19741 gcagataaaa aacatcttca tcaccgacta atcagtttag gctttactca taaaggtgct
    19801 gtcctgacga tttatgcgat ggctttgatg ttttctttca cagctatggt catgaattat
    19861 actggaagaa tcggaacgat catattgatt attgccatgt tgtttgctgc aattttatta
    19921 tttgaattga ttggtctgat caatgagaaa catcagttta ttttaaaaac attgcgattt
    19981 ttagggaata aagagtaccg tacgcaagta aaacagaaat atttgaagaa atggaaccgt
    20041 catatagatg aataacaaat taaaccagcc gatccttatt agtattgtga cggcaaacag
    20101 tgataaaatt tttcggacac tagataattt tatttcaact gtaaaagatg aaacagcagt
    20161 aaaaatccga atttttgaca acaattcact tcccgagtac aaaaaaagac taacagagta
    20221 taggaaatat ccctacatcg atatctattt tcatgatgaa aattccggat ttggttatgg
    20281 acataatcac aatctgatag ctagcgattc tcgctatggc attatttgta acccagatat
    20341 tttagtggat gaagagacag ttacagcatt ggtcttgtta ttgaaagaac atccggaatg
    20401 tgcgatgtta gcacctaaga ttcttaatga agatggaaca actcagcacc tcatccgtga
    20461 gcagctggct gtttttgatt acgttttgcg ctttgtgcct ttcaaatggg tcaaaaaaaa
    20521 gtttgacaaa cgactggctg tgtttgaatg ccgaggattg cctactgatg ctaatagtta
    20581 tgtacggatg atctcaggta gttttatggt agctgatatt gataaattta aagagataaa
    20641 ggggtttgat gagcgtttct tcatgtactt tgaagacaac gacttgtgtt tgagttttga
    20701 aaaagcaggt tacaagttat tgtacacacc cttttatgca gttatccatt tttacgggaa
    20761 gggtgcgcat cgaaatgcaa aactgtttcg cgtgtttttg acttctatga tgaaattttt
    20821 caataagtgg gggtggcgtt ttttctgatg aaaccagaaa tattagtagt cgttgtgctt
    20881 tatcagcaac tattcagtca atctccagct tatggaccat taagcaaagc acttagcgaa
    20941 aagcaaattc agcttattat ttacgataat agtcctcaaa ggcagcaaaa tgagctgttt
    21001 gaaaaagaaa atatcgttta tcatcatgat ccgcaaaatc ctggtttagc ggtggcttat
    21061 aattttgcgt tgtctcaagc agatgaagga acacgttgtc tcgtcactct ggatcaggat
    21121 acgaggctag tcgacgatta ttttgaacta ttgcgtaaag taacctttac agatgaatgt
    21181 gttgctgcag ttccgatgat tttttctggg agccgacaag tttctcccct atattcagat
    21241 cgttatatca atcgtacagc tcaagcagta gaagttgaag gcccaacagc tgaacgaatc
    21301 atggcaatca attcaggtgt tgcctggtct gtcaaatttc taaaaaagat tggcgggttt
    21361 aatcctgctt tttctttaga ttttttggat cattggctgt tttggaaagt ggatcagttg
    21421 gaaaaaactg tggagatcct tcctgtacga atggaacatg atctttcggt tttagactat
    21481 gaaaaagttt caagtaatcg ataccgttct attttacaag cagaaaatcg attttatcat
    21541 gaatatgatc aaaggcattt atccagtcac cgacgacaat tattgttaag aacagctaaa
    21601 cagttcttga ccgtcaaaaa ccggcatatc tggcggatga ctttgcgctc atatgttaag
    21661 aattggaagg tgtgaattcg tgcgttcggt ttgtatagct acctttaatg gaggcagata
    21721 tttaagagag cagttagata gtattttatc tcagctttca gcagaagatg aagtagtcat
    21781 ttctgatgat gggtcagtag atgatacatg ggatatatta actgaatacg cagaaaaaga
    21841 taagcgaatc cagctttttt cggggcctcg tcaaggattg atcgctaatt ttggttatgc
    21901 gatccagcag acgaaaggag atctaatctt tttggcagac caagatgatg tttggttgga
    21961 gacaaaagtg aaaaagatca ctgactattt tgagcagaat ccggatcagc tagtagtagt
    22021 ttctgattta atcattgtgg acagcgagct gcaagagatc catgattcgt atttttcgtt
    22081 tcgaaattcc aaagaaggat ggcttaataa tgtgataaga agtcactata ttggtgctgg
    22141 tatggctttc cggtcttctt taaaacccga tattttgccg atcccgtcag aagtgccgat
    22201 gcatgatatg tggattggtt tgctgggagg aaagaagact gggttcctaa gagaaccgtt
    22261 aactctgtac cgaagacatg atttaaacgc gagtgagatt gaaacacatt ctaaattacg
    22321 gcaaaaaatt tcttggcgtg ttcatctgat tacggcgttg atcaaacgaa aatggtttaa
    22381 acgttgataa aagatgttta aatccttaat atttccatga caaatataac aattgatgag
    22441 ataataacaa agtgaaattt gaaatcaaaa aggagagcag atatccatga agggaatcat
    22501 tcttgctggg ggcagcggaa cacgtctgta ccctttaaca aaggcaacat cgaaacaatt
    22561 gatgccgatt tatgacaaac caatgattta ttatccaatg tctactctga tgttggcggg
    22621 aatcaaagaa attctgatta tttcaacgcc acaagataca ccacgtttca aggaattatt
    22681 tggaaacgga aatgatttag gactgcagat cgaatatgca gtacaagaaa gtccagatgg
    22741 attggcacaa gcgtttatca ttggagaaga atttattgga gatgatagtg tctgcttggt
    22801 attaggggac aatatttact atggtggcgg tctgtctaaa atgttgcaac gagcagcttc
    22861 aaaagataca ggcgcgacag tatttggcta tcatgtcaat gatccagaac gctttggtgt
    22921 agtagaattt gatgagaata tgcaagcttt gtcgattgaa gaaaagcctg ctcagccaaa
    22981 atcaaattac gctgtaactg gcctttattt ttatgacaat gaggtaattt ctatcgctaa
    23041 aggtatcaaa ccttcagaac gtggagaact agagattacg gatgtcaata aagcatattt
    23101 ggataaagga aaactatccg tagaacttat gggacgcgga tttgcttggc ttgatacagg
    23161 aacacatgaa tcattgcttg aagcatctac ctttatcgaa acaattgaaa aacgtcagaa
    23221 cttaaaagtg gcttgtttgg aagaaattgc ttaccgtatg ggctacatcg ataaggaaca
    23281 attgctggcc cttgcacagc cattgaaaaa aaatcaatac ggtcaatatt tattgaattt
    23341 ggccgctgaa taaggagtcc ttacagatga aagtaacaga tacaaaatta acagatgtca
    23401 aaatcgtaga aatggacgtc tttggcgatc atcgcggttt cttcacagaa agttattcaa
    23461 aagcaaaatt tgcagaacat ggattgaaat atgactttgt tcaagacaac cattcactat
    23521 cagctgaagc tggtgtcctt cgggggcttc atttccaaaa aggagaagct gcacagacaa
    23581 aattgattcg ggtagtcact ggagcagttt tagatgtagt agtggatatc cgcaaaggta
    23641 gcccgactta tggtcaatgg gacggattta tcttatctga acataatcat cgacagttgc
    23701 ttgtacctaa aggatatgcc catggattcg ttactttgac accaaatgtg aacttccttt
    23761 ataagtgtga taattactat aatgcagaag cagatggggg catcgcattt gatgatccag
    23821 atttagcgat tgattggcca attgatccat caagagcgat tatgtcggaa aaagatcacc
    23881 gtcatccgac attaaaagaa tttgaagcag aaaatccatt tgtttacgga gaaatctaag
    23941 aattaaggag aaattttctt atgaaaaaca tcattgttac cggaggtgcc ggattcatcg
    24001 gctctaattt cgttcattac gtggtaaaca atcatccaga agtgcatgtc actgttttgg
    24061 ataaactgac ttatgcagga aacaaagaga acttggccgg acttcctagc gatcgcgtag
    24121 aattggtcgt aggagatgtt gcagatgcag aattagtgga ccgtctagta aaagaagcgg
    24181 acgcagttgt tcattacgcg gcagaatccc ataacgataa ttcattaaaa gatcctttcc
    24241 catttgtaca aacgaatatc attggtacgt atacactttt agaagcttgc cgcaagtatg
    24301 atgtacgcta tcatcacgta tctactgacg aggtttacgg cgatcttcca ttacgtgaag
    24361 acctgccagg acatggggaa ggcgaaggag aaaaattcac tgcggaaaca ccatataatc
    24421 cttcaagtcc atattcttct acaaaagctg gttcagatct gttagtaaaa gcatgggtgc
    24481 gttcttttgg cttacgtgca accatttcaa actgttcaaa caactatggt ccttatcagc
    24541 atattgaaaa atttattcct cgccaagtaa cgagtatctt aagcggtatt cgtccaaaat
    24601 tatatggcga aggaaagaat gttcgtgact ggatccatac gaatgatcat tcttcagcag
    24661 tgtggctgat tttgacgaaa ggcagaattg gcgaaaccta tctgatcggt gcagacggtg
    24721 aagaagataa taagaccgtg atggaattga tcttagaaat gatgggccaa ccaaaagacg
    24781 catacgacca tgtaaacgac cgcgcagggc acgatttgcg ttatgcaatc gattcgacaa
    24841 aattgcgaga agagctaggt tggcagcctg agtttacgaa cttccgtgat gggttggctg
    24901 acacgatcaa atggtacgaa gaacatgaag actggtggaa aaaagaaaaa gaggccgttg
    24961 aagcagcata tgccaaaaat ggacaataat acagaggaaa tttcactgta ttaatggaaa
    25021 aacacatgtg aggcaggcgc tcgcctgtct cttttttaaa ataaaaatca aggagcggat
    25081 aaccatatgt ttttattaac tggaggaaat ggacaattag gtacagaatt gcgccattta
    25141 ttagatgaaa aaaatctgcc atatgtttca acagatgcaa aagacttgga tatcacagat
    25201 gctgagaaaa caatggctta tattacagaa ttaaaacccg atgttatttt ccattgcgca
    25261 gcttacacag cagtagataa agcagaagaa gaagcaaaag aattagatga gaaaatcaat
    25321 gtcgatggca caagaaatgt agcacttgca gcaaaagctg tgaatgcaca attcgtctat
    25381 atcagtacag actatgtttt cgatggaaaa aacaaaaatg tagaatacga agtagacgac
    25441 caaacgaatc cactaaatga atatggtcgt acgaaattat taggcgaaaa agcagtagaa
    25501 gagattttag aagactatta tatcatacga acttcttggg ttttcgggat ctatggacac
    25561 aatttcgtct tcacgatgca acgcctagcg gaaacccgcg acaaactgac tgtagtaaat
    25621 gaccagtttg ggcgaccaac ttggacacgt actttggcag aatttatggt atatgtcatc
    25681 gaagaaaaag caccatttgg tatttatcat ttgtcgaacg aaaatagctg ttcttggtat
    25741 gagttcgcaa aagaaatatt gaaagataca gatgtagaag ttgcacctgt tacgtcagaa
    25801 gaatttccgc aaaaagcaac acgtccgcag tattcagtca tgagcctgaa aaagacagaa
    25861 gcgttaggtt ttgtcatacc aacttggcaa gaagcacttg cgcaaatgct ggaagctgcc
    25921 aaaaaaatga ataagtaatt tcctttttat caaatagaat ttggagcatg atatggaaaa
    25981 acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg aatcaggcag attcgaattt
    26041 ggtgcttgac ctgtttctaa aactccgcta tcttattggc gttttagtca tcgtggtcat
    26101 cgttactttt aatttaaacg gaagttcact tggtgtttgg gataaatatg tgtcgcaaag
    26161 agacgatggg aaaaaaactg atgtgatttt tggacagaat cgggaagtgc ggtcagacga
    26221 atggctggtg cagacacctt tttacctctc acaagctgaa gaggactttt cgttggtgaa
    26281 cgaggattat tcattaaacg gacaaaacat gatcattgcg tataattctc ctgtaaagga
    26341 tattacggtt atcggaaaac cattcaactg gggattcttt ttcctcggaa gagatcgagg
    26401 actttccttt tattgggcga tgaaattagt cgtcatggtc ttgcttggat tcgaagtact
    26461 gatgatatta acgaagagga aaaaagctct atcgctaatc ggtgcatttg cgctgacatt
    26521 ttctccagca gtccagtggt ggttcatgca acatgtgggc gatttgattt tctttacttt
    26581 gggactgatg attgcttttt atcattattt ctacaatcat gataaaaaat ggctacgaac
    26641 gttgatgatg cttttggtgg ttattttcgg tattgggttt attttagtga tttacccagc
    26701 ccatcaagtg atgctggcgt acttgctgat tttttatttt gttggcctgc ttatttacta
    26761 tagcaagaaa gtttcatggg atctgttgga tgtagtgttg attggagggg cagttttatt
    26821 tattggcgga gtcatggttc atttctggct aacgtcaaaa gacgcattgt tagcttcttt
    26881 gaatacctta tatccaggaa aacgggtaag tactggagga aagtggacga ttggcgaatt
    26941 tttttctttc cttacaaatt ggaaaattcc ttttgaagat atcaactatt cgaataactc
    27001 tgaagtggcc ctgttctatc acttttttcc aactgtattc ctagcaagtc catttgtgtt
    27061 atttggcaaa aaagatagcg aacaaaagtt actgggacgt attttgatga ttttttgtct
    27121 gttttctatc ttttggatta ccgttgggtt gccaaaatgg ctagcagaaa tcactctgct
    27181 gtcttatgtt cctccattaa gagccatcct tacttttagt tttgcggctt gtttattatc
    27241 gatctggttt atcgcttata tttggagaga agatgttatt cctatttggt ataaattcat
    27301 tttgttggca gtaaatgcat ggtcttattt ctatgcgatt acgcattcga aaatggataa
    27361 ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg ggaacagtga ttttagcttt
    27421 tgcattattt ggctggagac gcttgtttta cctcgctttt gtcgccttag ttatcgtatc
    27481 tggctttttc gtcaatccag tcgtagaagg tacaggagcg atttttgaaa aaacactggc
    27541 aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa gtttggttgt ctgaagatga
    27601 attatataac ttcaccccaa cattgggagt gaaatccttc aatacagtac gcttttatcc
    27661 agatatagct gcatggaaga aaatcgatcc gaaagaagaa tacgagaagt attataatcg
    27721 gtatgctcat acaagagctt ttatcgctaa ggaagataca aagttcattt tagatcgtcc
    27781 agatatgttt tcggtcacgt tgaattttga agacgcagaa aaattaggga tcgactatgt
    27841 gatcagtaaa cgaccattaa ctgattacaa ccaattatat gaagcgcagt tcacgcagct
    27901 atatggaccg gataaagacg gttatatgat ttttaaagta gaatatccgg attatccaga
    27961 tgtaccacaa acacaaactc aggaaaatca gctagtcgta cagtaatagg ctagctgaga
    28021 ataatcctga aatattagta gaaaggagag tgaagataat tcaagcaatc gtacaaaaat
    28081 ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttgct gacgctagtc ttttttgttt
    28141 ggacagcagg ggtaggtttt ccagcaactt tgtttatata taacaatagt tcattactta
    28201 tcgcttttag tgtggctatt ttgctcattt taaatgttta taaagcaaaa ggcgtcgatt
    28261 ttctttatct aggtgttgcg cttgcccttt tcattttctt ttcttacttt cgggaattaa
    28321 gaaatgagtc tttatttgga gacttactgt ttcctttgat tattttattt tgtttagtaa
    28381 ttaaatggat cgagtggaca aaatgggatc gcggtattta tctgattgtc tttacggtct
    28441 tcctcggaat cacgttatat cgtgtattta cagagatcca agtaccagaa ggacaaagca
    28501 tctgggctcc aggcaattcg ctttctgata tttggatcaa tacgaataca atcggaagtt
    28561 ctttgatgac tcttgcttta ttgatcagtg gatttgccag ttcttttgaa cgatggtatg
    28621 ttcgaatcat cggtgggata gcagtcgttg cagccttttt aggtatttgg gtctgccaat
    28681 ctagaggtgc attagtcgcg ttgattgttt ttgcgatatt ggatctttta cctaaatcgt
    28741 tgatgcgtgt cattcgtgcg ccatttattg cctacacggt tacgattctt ttagctttgc
    28801 cgatttctta tctggcggct gtctctgaaa aagtcaattt gtttaccggt cgagaagata
    28861 tttggcataa attctatcag acgctaggag aaaaatctga acaagtttta ctaggaatga
    28921 aaacatttat cttccagcga ggaaaccaat ttttaggaaa ccacaatagt tataattcta
    28981 ttttgaatat ctatggattg ataggatttg ggattgctgc tttgctgctt atccttttta
    29041 ttggtcgctt aacgttgaaa gcagatttat ccaacgggca gatgactttt atctgggcct
    29101 tttttgcggt gatgatgcag tcatttatgg aggatacatt gacttcatac cgatgggttc
    29161 cgattattta tatcctgttg gctatggccg ttcatcgata tgatgaacca gaagttacgg
    29221 aggaattcta tgttatggaa gaggcatatg acgatatgac ttatgatgat acatcttatg
    29281 aggaacaacc agatagcgct cattcttcca ggatccaacg ctatcattga taggcacaaa
    29341 atacgagaga ggctgtgaca accgtcgcag ccttttttgg tttattgcaa ttgtctagca
    29401 tcttctgtaa aatagaaacg ttagagaaaa tcttgtatca tagaaaatta agaaagaaat
    29461 aagaacgcag ctcggtacca aaaatatggt aaaatggcac ttgactgtaa ttcactgaaa
    29521 atcgcttgga ggaacacaaa tgtttaaaga tttgtattta tttatgaagg acatttatca
    29581 gaacagaaaa ttactgctac agttttcgat caatgacttc aaatcaagat atgcaggatc
    29641 gtttttagga atcttatggg cgtttatcaa tccgctattt actgtattga tttattggct
    29701 ggtatttggt gttggactga aagcacaaat gatcgatgca aaatacccat ttatcgtata
    29761 tttagttacc gggatgattc cctggttctt cttctcagat gcatttgcat caactacggt
    29821 tgtttttcgg gaatatactt atttagtaaa gaaagtcgta ttcaatattc gtatcttgcc
    29881 cacgacgaaa atcctatcca atctgtacac ccatttattc ttcattctga ttggcatagc
    29941 tgtcgcgctt ataaatggta tatatccttc agcaatgtct ttacagctga tttattattt
    30001 attttgcatg tgtgctttct tgacaggatt gacttggctg acggcatcga tccaaccatt
    30061 tttacctgat atcatgcaat taattacgat cgtgctgcag ttgatcatgt ggacattacc
    30121 gatattatgg agccctaatc agtttgattc aggtcttgta agcatattga aattaaatcc
    30181 gctgtactat gttgttcaag gttatcgtga gtcattttta agtgaagctt ggttctggga
    30241 gcatggaaca tatactttgt atttctgggt tttcacgtta gtaatgttcg taatcggctc
    30301 tgcagtgttc cgtagattga aaccacattt ttcagatgtg ctgtaaggag aatagctatg
    30361 acagaatatg caattagact aaaagatatc acaaaatctt ataatatgta cgcaaaacct
    30421 tcggatcgat ttcgtgaagc cctcaatcct tttaagaaat cctatcatga tgtattttat
    30481 gcgttgaaaa acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt cgtgggagaa
    30541 aacggatcgg gaaaatctac aatgttgaaa atcattacgg gagtactgac accaacatcc
    30601 ggcacgatgg aaatcgaagg aaaaatctca gctttgctag aactaggatc aggattcaat
    30661 cctgagtatt ctggctatga aaatatctat ttaaatggaa tggttctagg attttcaaga
    30721 gaagaagtcg atgaaatggt cgatgatatc attgaatttg ctgacatcgg cgaccatatt
    30781 tatcaacctg taaaaaccta ttcaagtggg atgttcgttc gtctagcttt tgctgtagcg
    30841 atcaatgtga atcctgatat tttgattgtg gatgaagcat tggcagtagg agatcttgaa
    30901 tttcaactaa aatgtatgga aaaatttaca gaaatcaaga actcgggaaa gacgatttta
    30961 ttcgtttctc atgatattag ttcgattcgc cgtttttgtg atcgttccta ttggttgaaa
    31021 aatggaaaag ttgtcgaata tggtgatacg atggaagtaa cgacgaatta cgaaaacttt
    31081 ttgaagaaaa aatcagttaa aacagtagat cgaaataaaa accaagtaga acgcttcgca
    31141 gctccagata tcgtagacgt gacgaaagcc gaattgttgg ataaagataa aaatccagtg
    31201 gatatgctgg aacaaggaga aaaactatac gttaaagtaa cctatgaagt aaaagacgat
    31261 acaatcaaaa atccagtttt aggaattgct ctgcgtacag tcgacaattt ttatgtgtgc
    31321 ggactcaata ctctactaga tggtatcaag atcccttgga aaaaaggtga gaatatcttt
    31381 tatctggagt atgaaaagtt aggtttgtta gctggtgaat attattttga cgttgcggtc
    31441 tttgaagaaa atgcaacggt tcctttggta tacaagacaa aatacatgaa cttgtttgtc
    31501 agcggttctt atatcggaga aggcatcgtg gtcttagacc ataaatggga agaagggact
    31561 catagcaatg aaatatgatt ttgaaatgga cctagatgaa caaagcagtg tgggaaaaat
    31621 tgccgcacag atcaaaccag gaagtaaagt tttagagttt ggtcctggaa acggccgatt
    31681 aacgaaacat ttgatcggag caaaacaatg tgaagtgagc attgtcgagc tagataagga
    31741 attgttcgat tttgtcagcg agtttgccca agatggattt tatggagata tcgaaagttt
    31801 tgaatgggca aactattatg ctggacaaac atttgattac gttttgtttg ccgatgtttt
    31861 agaacatttg gtagatcctg gaaaaacgtt gaagaaagtt agagaatttt tgaatgaaga
    31921 aggagaaatt ctaattacat tccctaacct tgcgcataat tctgtcatga ttgatttgtt
    31981 caataatcaa ttgccatggg cttcttatgg tttgctagat gaaacccata attcttttta
    32041 tactcatgat ggcttccaaa aagtttttga aaaagcagga ttatacatca atattgaaga
    32101 ttatttgtac ttagctgtag gtgatacaga attaaaatca acttatgaag aattgccgga
    32161 agctgtacgt tatgatttca aaatgcgacc atttggcgaa gtctaccaat acttcttctc
    32221 attgatgaaa catccagtag cacaaagttc cattgcgcag ccgcaaaatt ctaattatgt
    32281 gaaagtatta gaagtaatcc agcaaacgaa acaagatgaa ataattcaac aaataccatt
    32341 caataatttc acaggggaaa atgagacact aagtttccat gtatcagaat tagcagaacg
    32401 aatggtgttt cgcttcgcgc agcaacctag ctttatcgaa ttcagcgcag aagctgctgg
    32461 agaaaaactc acatttatcg attcaaatgc tgtagtcaaa acagtaaatg attgttattt
    32521 gtttgatggt aaagatttac cagagttctt tttaacggat attgcaggga aagaagtgac
    32581 tatccattgt cactatcgtt ttatcggaga attgacacca acaatgaaag aattgctgga
    32641 aacaatccgt ccaatggctg aagttacaaa gcagctttct gagaaaaatg atgaattaga
    32701 aagagaaaat cattatctta ttgagaataa tacaagatta gatcagacgt tgaaaacaac
    32761 cataaatcgc tattgcacgt tgttggaaag tgatgaatgg acaatcaaac atagattagg
    32821 gcgacgtaag aaagaaacat cgaaaaaaat ccaagaaaaa gagctttctc tatgtattga
    32881 cgaaaaaatc tgggatgcag aaacaaagat attgaaaatc atcgggtggg gaatcgctaa
    32941 ttcggatcgc caaccattgt cctataagtt atcagccgac caatcaccat tttttgaagc
    33001 aattcctgtt ttacgagaag aagtcaatca agcaaaacag ctagctgaag gagcaaaagc
    33061 aggatttgaa ctgcggatcc tttgcgagaa ggaacgttca ttcttagttg aagcagtggc
    33121 tcaaaatggt caatcatgga tcattgaaat gtaaataatg attaacttat aaagataagg
    33181 agtaaacgaa tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat cactcgtgaa
    33241 cgggccactg gcgatttgct tattgtgggc tgggcaattg atgaagtgac caaagaaatc
    33301 cctacgataa aggtagaaaa agaaaatgtg attgctgaag caacacatgt cgtaagattg
    33361 gatatcaatc atttatataa tcttgatgtt aagacccaaa gcggttttaa ggtacgttta
    33421 tcagggaaaa tgagaggaaa agcaatctta gacttccaaa cagcaagaca tcaaaatggc
    33481 atagctgtga aattaaacgg taaatatcct tatgatgacg gcattgaatc ttcatgggaa
    33541 agaaaaaaaa gattactgaa aaaagggata aattatgcaa ggactcatgg cgtaaaaaaa
    33601 gcaatccgcc gggtcaaact ggaattgaaa ccaggatcga tcgactatgc agagtggatc
    33661 agtcgtcatg agaaacctga tctgaaagag caaaggaaag aagtacaagg atttgcctac
    33721 cgcccattga tttccatact tatgcctgtc tataacgttg aaatcaaatg gctcgaaaaa
    33781 tgcattgatt cggtacttga tcaaacgtat gatcactggg aactatgtat cagtgatgat
    33841 gcttctactg atccagctat ccgaaagtgt ctagaatcct atcaagcaaa agatgataga
    33901 attaaagtcg ttttccgaca agaaaatgga cacatcagtc tggcaactaa ttctgcattg
    33961 gaaatggcag aaggagagtt tatagcgctc ttagataatg atgacgaatt acctccattt
    34021 gccttatatg aagtggcaaa agtgctaaac gtacatccag aactggattt gatctacagt
    34081 gacgaagata aaatcgatgc agatgggaat cgctttgacc cgcacttcaa agcagactgg
    34141 tctcccgata cattgatggg aaataattat atctctcact taggcgtata tcggacaagc
    34201 attgttaagg aacttggcgg tttccgtaaa ggatatgaag gctctcaaga ttatgatttg
    34261 gttcttcgtg tgacagaaca aattcctgca gaacacattt accacattga ccgtgtcttg
    34321 tatcattggc ggacgatccc gggatcaact gcaagcaatg gagaagctaa aagctacatc
    34381 tatgattctg gagtgaaagc attgacagat gctctatcta gaagaaatat caagggaagc
    34441 gtacatcctg gcagaatttc tggtttttat gaaatcactt atgatgtttt gcaagaagac
    34501 ttggtcagtg tgatcattcc aactaagaat ggctatgaag atctaaaaac ttgtgtggat
    34561 tcgatcatcg aaaaaacgtc ttatccaaat tatgaaatca ttattgcaga taatggaagt
    34621 acagatccta aaatgcaaga attatttgca gaatacaagc atcagctgaa agaccgattt
    34681 atcgtcgaat taattgacat tccattcaat tattcacgta tcaacaatct ggcagcagaa
    34741 aaagcaaacg gcaaatactt tttattcttg aataacgata cagaagtgat tgaacctgac
    34801 tggatgacag cgatggtttc ttatgcacag tttgatcgaa tcggctgtgt aggagcaaaa
    34861 ttattttatc cggatgatac aacgcagcat gcaggtgtat tattagggat cggtggcgtt
    34921 gcaggacatg cattgaataa ctatgatcga acacattgtg gttactttgg ccgattggtc
    34981 attgacgtca attacttagc agtcactgca gcttgtatga tggtcaaggc agctgacttc
    35041 aatgctgtca atggatttga tgaaatatta gaagtagcat tcaacgatgt tgacttgtgc
    35101 ttgaaagttt atgaactggg tcgttacaat gtctatgcac atcaagcaga attgtaccat
    35161 ttcgaatcta aatcaagagg ttatgaagat acaccagaaa aacaaagacg ttttgctggg
    35221 gaaatcaaaa aaatgcaaga taagtggcct tcttatattg ctcatgaccc attttataac
    35281 gataatctaa ctaaagaagg aatcggcgat ttttcgttga gacctgattg attaaaagaa
    35341 ctgtattttc atttttatta atgaaaatac agttctttta atttaatt
//
LOCUS       DAZKSU010000024        34397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000024 DAZKSU010000000
VERSION     DAZKSU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.024"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8Z3_001203"
     CDS             352..1041
                     /locus_tag="ACG8Z3_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0823533.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8Z3_001204"
     CDS             1028..2236
                     /locus_tag="ACG8Z3_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0823534.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8Z3_001205"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8Z3_001205"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0823535.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8Z3_001206"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8Z3_001206"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0823536.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG8Z3_001207"
     CDS             complement(6339..7034)
                     /locus_tag="ACG8Z3_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0823537.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8Z3_001208"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG8Z3_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0823538.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG8Z3_001209"
     CDS             complement(8233..8424)
                     /locus_tag="ACG8Z3_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0823539.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG8Z3_001210"
     CDS             complement(8445..9335)
                     /locus_tag="ACG8Z3_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0823540.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG8Z3_001211"
     CDS             complement(9322..10089)
                     /locus_tag="ACG8Z3_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0823541.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8Z3_001212"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG8Z3_001212"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0823542.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8Z3_001213"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG8Z3_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0823543.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8Z3_001214"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG8Z3_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0823544.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG8Z3_001215"
     CDS             14660..15043
                     /locus_tag="ACG8Z3_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823545.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8Z3_001216"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG8Z3_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0823546.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG8Z3_001217"
     CDS             complement(15987..16805)
                     /locus_tag="ACG8Z3_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0823547.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8Z3_001218"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG8Z3_001218"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0823548.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8Z3_001219"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG8Z3_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0823549.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8Z3_001220"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG8Z3_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0823550.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8Z3_001221"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG8Z3_001221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0823551.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8Z3_001222"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="ACG8Z3_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0823552.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="ACG8Z3_001223"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="ACG8Z3_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0823553.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8Z3_001224"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="ACG8Z3_001224"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0823554.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8Z3_001225"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="ACG8Z3_001225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0823555.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8Z3_001226"
     CDS             26847..27146
                     /gene="rpsF"
                     /locus_tag="ACG8Z3_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0823556.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8Z3_001227"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="ACG8Z3_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0823557.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8Z3_001228"
     CDS             27734..27970
                     /gene="rpsR"
                     /locus_tag="ACG8Z3_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0823558.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="ACG8Z3_001229"
     CDS             28113..30086
                     /locus_tag="ACG8Z3_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0823559.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFTAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8Z3_001230"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="ACG8Z3_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0823560.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8Z3_001231"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="ACG8Z3_001231"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0823561.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            32965..34257
                     /locus_tag="ACG8Z3_001232"
     CDS             32965..34257
                     /locus_tag="ACG8Z3_001232"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0823562.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10495 a         6692 c         6807 g        10403 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tactgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 agggttcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cgattttatt tgaaatatgt atctattatt gatacaatgg ttcaggatta
    32941 aataatatag aacgaggtgt tcacatgtca tctgtagtag tagtaggtac gcaatggggc
    33001 gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa atgctgaagt gattgcacgg
    33061 taccaaggtg gcgacaatgc agggcacaca attaagttta acggtgtgac ttataaactg
    33121 catttgattc cttctgggat tttttataaa gacaagatca gtgtgatcgg aaacggcgtt
    33181 gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt tacatgaaaa aggtgtagaa
    33241 acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct tgccttatca tatcaaattg
    33301 gaccaattac aagaagatgc taagggcgaa aataagattg gtacaacgat taaaggaatc
    33361 ggacctgcct atatggataa agcagctcgt gtaggtatcc gtattgcaga cttattagat
    33421 aaagaaattt ttgaagaacg tcttcgtttg aatctagatg acaaaaaccg tcaatttgta
    33481 aaaatgtttg attcagaacc acttgatttt gacgatattt ttgaagaata ttatgaatac
    33541 ggacaacaaa tcaaacaata cgtcacagat acttccgtta tcttgaatga tgctttggat
    33601 gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa tgctggatat cgaccaggga
    33661 acttatcctt ttgttacttc ttctaaccca gtagccggtg gggtaacaat cggaagcggt
    33721 gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca aagcctatac gtcacgtgtc
    33781 ggtgatggac catttccaac tgaattattt gatgaaacag gagaacaaat ccgcgaagta
    33841 ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg taggctggtt tgattctgta
    33901 gttatgcgcc attctaaacg agtttcaggt attaccaacc tatctttaaa ctcgatcgat
    33961 gtattaagtg gattggaaac agtaaaaatt tgtacggctt atgaattaga tggcgaacta
    34021 atctaccact atccagcaag cttgaaagag ttgaaccgct gcaaaccaat ctatgaagaa
    34081 ttgccaggct ggtcagaaga cattacagga tgcaaaacac ttgcagaact gccagaaaac
    34141 gcacgtaact atgtacgtcg tatctcagag cttgttggtg tacgtatctc aacattttct
    34201 gtcggacctg atagaacaca aacaaacatc ttagaaagcg tctgggcgca aatctaagga
    34261 taaaaacgac gactgaatca ggacgttctt accagagcga gtcgaagtgt ttatctatac
    34321 gataataata acttgaacac tgtttattca gaataaggga ctgtgacaaa aaccgtgtca
    34381 cagtcccttt ttccgaa
//
LOCUS       DAZKSU010000025        32924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000025 DAZKSU010000000
VERSION     DAZKSU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.025"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            558..1171
                     /locus_tag="ACG8Z3_001233"
                     /pseudo
     CDS             558..1171
                     /locus_tag="ACG8Z3_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406632.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1920..5069)
                     /locus_tag="ACG8Z3_001234"
     CDS             complement(1920..5069)
                     /locus_tag="ACG8Z3_001234"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFQ0823563.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(5082..6299)
                     /locus_tag="ACG8Z3_001235"
     CDS             complement(5082..6299)
                     /locus_tag="ACG8Z3_001235"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFQ0823564.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            complement(6289..7884)
                     /locus_tag="ACG8Z3_001236"
     CDS             complement(6289..7884)
                     /locus_tag="ACG8Z3_001236"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFQ0823565.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            complement(8394..9176)
                     /locus_tag="ACG8Z3_001237"
     CDS             complement(8394..9176)
                     /locus_tag="ACG8Z3_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823566.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(9241..10077)
                     /locus_tag="ACG8Z3_001238"
     CDS             complement(9241..10077)
                     /locus_tag="ACG8Z3_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0823567.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(10095..11504)
                     /locus_tag="ACG8Z3_001239"
     CDS             complement(10095..11504)
                     /locus_tag="ACG8Z3_001239"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0823568.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(11518..13341)
                     /locus_tag="ACG8Z3_001240"
     CDS             complement(11518..13341)
                     /locus_tag="ACG8Z3_001240"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0823569.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(14184..14510)
                     /locus_tag="ACG8Z3_001241"
     CDS             complement(14184..14510)
                     /locus_tag="ACG8Z3_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823570.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(14556..14918)
                     /locus_tag="ACG8Z3_001242"
     CDS             complement(14556..14918)
                     /locus_tag="ACG8Z3_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0823571.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(14955..15170)
                     /locus_tag="ACG8Z3_001243"
     CDS             complement(14955..15170)
                     /locus_tag="ACG8Z3_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0823572.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            15297..15633
                     /locus_tag="ACG8Z3_001244"
                     /pseudo
     CDS             15297..15633
                     /locus_tag="ACG8Z3_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(15709..17706)
                     /locus_tag="ACG8Z3_001245"
     CDS             complement(15709..17706)
                     /locus_tag="ACG8Z3_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823573.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(17804..18079)
                     /locus_tag="ACG8Z3_001246"
     CDS             complement(17804..18079)
                     /locus_tag="ACG8Z3_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823574.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            18311..19045
                     /locus_tag="ACG8Z3_001247"
     CDS             18311..19045
                     /locus_tag="ACG8Z3_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0823575.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEKFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            19084..20226
                     /locus_tag="ACG8Z3_001248"
     CDS             19084..20226
                     /locus_tag="ACG8Z3_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0823576.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(20321..21712)
                     /gene="sufB"
                     /locus_tag="ACG8Z3_001249"
     CDS             complement(20321..21712)
                     /gene="sufB"
                     /locus_tag="ACG8Z3_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0823577.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(21717..22187)
                     /gene="sufU"
                     /locus_tag="ACG8Z3_001250"
     CDS             complement(21717..22187)
                     /gene="sufU"
                     /locus_tag="ACG8Z3_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0823578.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(22174..23409)
                     /locus_tag="ACG8Z3_001251"
     CDS             complement(22174..23409)
                     /locus_tag="ACG8Z3_001251"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0823579.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(23406..24692)
                     /gene="sufD"
                     /locus_tag="ACG8Z3_001252"
     CDS             complement(23406..24692)
                     /gene="sufD"
                     /locus_tag="ACG8Z3_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0823580.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(24705..25478)
                     /gene="sufC"
                     /locus_tag="ACG8Z3_001253"
     CDS             complement(24705..25478)
                     /gene="sufC"
                     /locus_tag="ACG8Z3_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0823581.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(25675..26523)
                     /locus_tag="ACG8Z3_001254"
     CDS             complement(25675..26523)
                     /locus_tag="ACG8Z3_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0823582.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(26547..27233)
                     /locus_tag="ACG8Z3_001255"
     CDS             complement(26547..27233)
                     /locus_tag="ACG8Z3_001255"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0823583.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(27226..28260)
                     /locus_tag="ACG8Z3_001256"
     CDS             complement(27226..28260)
                     /locus_tag="ACG8Z3_001256"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823584.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(28323..28579)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28655..28993)
                     /locus_tag="ACG8Z3_001257"
     CDS             complement(28655..28993)
                     /locus_tag="ACG8Z3_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0823585.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(28995..29351)
                     /locus_tag="ACG8Z3_001258"
     CDS             complement(28995..29351)
                     /locus_tag="ACG8Z3_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0823586.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(29357..30544)
                     /locus_tag="ACG8Z3_001259"
     CDS             complement(29357..30544)
                     /locus_tag="ACG8Z3_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0823587.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(30735..31580)
                     /locus_tag="ACG8Z3_001260"
     CDS             complement(30735..31580)
                     /locus_tag="ACG8Z3_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0823588.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            31814..32779
                     /locus_tag="ACG8Z3_001261"
     CDS             31814..32779
                     /locus_tag="ACG8Z3_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0823589.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         9638 a         6836 c         5541 g        10909 t
ORIGIN      
        1 cactaattgc tgtgattaat tacagaatat ggctctctgg gaaaatgtgc gcgtcatgta
       61 ttcaatttta aattctaatt tgtacaaaat gaatcgtcac gactcgatct gacgcaactc
      121 caaatggggg cgccgacctg taccaagtac agtggcatgt tctttaaaca tttgaagcaa
      181 aatctgtcca tcaaaaacct gtattttcaa agtgaacaag gtgcgctaaa tcaggaaata
      241 cttacactga tcatttctta atttacctac ttaaccaaaa tttaaatcaa tatgaaagca
      301 acacttttca aactaaagag gtctttttac cgcttgatgt ttgcttcgat aaaaaagttg
      361 atgtcggatt agttttgatt ttaataaaac gtactattcc tgaaaaaaat ttatccacta
      421 ctaagtattc ggatttttct aatttttaac aaaatttatt tcgaaatttt ctgatattat
      481 attcgcaaca ttagtgaaaa ttcttagata aattcattct ttcaatctcc gaattttaga
      541 tatatgtgaa atatctgttt tttgaatgtt tgcataagtt aaaagatgag cgtgctttag
      601 tattctttag aatagcttct tatacagggg aacgaaaagg agaaatactc ggactgacat
      661 gtagagacat agatttcgat aatcaaacta tctgcttcaa caaaacgtta gtagaactat
      721 ccgagggctc tctatgtatt aatcctacaa aaacagacaa atcaaaggga gtaatttcaa
      781 ttgataatgg aaccttacgt attctaaaaa atggaaacgt tctgtaacat aaaagaacct
      841 ttctagaggg attcggatag ataatataga tgatatacct gtaatcagca ctatcaaagg
      901 gaataaaaaa agggacttcc tttacaaagg ctaccctaac catgtcatgg ataaagtagc
      961 acggaagttc ccagatatga agataatcaa agtccatggc ttcagaaaga ctcatgctac
     1021 ccttctcttt gaagcaggtg ctcaagtaaa agatgtacaa gataggctag gacattcttc
     1081 ttctaaaaca atcatggaca tttatattaa agtatcaaaa aatcggcaaa atgaaacagt
     1141 cgaattattt gattcttaca tgaacggatg ataagcgagg tcaaaaacgg agtcagagga
     1201 tattatgggg ataccttttt acctcttaat cctttaatat caatccttta agcttcgact
     1261 tttgcaatct tcccttgttc gatatagacc caaatcgatc tatcaataat tatcaaactg
     1321 agtcatatag agacaaaaat ttcaaaaacg acctattaat tggtcatcga atatcattta
     1381 aaatcaatac gacggagtca aacacggagt caaaacaagc ccgctgagag taaaaaatat
     1441 acagtcccgt aggtaaagtg ccacaggcct gtatattttt tatttgagat tacctgaaat
     1501 gattccattt aatttttatc tcattttttc ctactagtta gtatcaaata ctctcctatt
     1561 ttctcaataa actattttgg gttacatggt atctgaaaca gctaaaacct tgtcattata
     1621 gcaaaaaatc actttatctc cgtttcattt cagatacatt tcacttaagc ggattccaaa
     1681 aaaacaaaag aaaaaggaac ccctagttag cctatgcttt ttattggttc ctctcaaaac
     1741 acggtcatat aacaacgggg tggggtaaat gaatgaaaaa ggtggaggaa acattattca
     1801 atagctgtct taaatacttc gttataaaat gtgaacaaat aaaagacatc tatccctatt
     1861 ttctacactt ccacttgact accatctttg aatcgtacaa ttttcttaac tcttctcgtt
     1921 tacctcaatt cattctcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc
     1981 ttcatttctt gtgggtattt aaatggcttc gcttctggat gtttggcttt atatccttcg
     2041 taatccttac tgttgataat tgctttcata ttaggaattg gatcttcacc tggtagatat
     2101 tgcagattag aagcatgtaa ttctttctca gaaacaaacc actcattgga aaagacatga
     2161 atcaaatcat cactcgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta
     2221 ctatctaaag tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct
     2281 tctgttttat tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc
     2341 gcagcataag ttcctagtaa ttgatcaata taagttgaat caatatcata caacttagtg
     2401 gtattttcac tataaaattc gatcgttgaa aaatcttctc cttctttttc tggttcttcc
     2461 tctattaatg aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt
     2521 tgattgttca aagttggaga tttcaccatg tcatcattgt aatcgtcata agtcactaac
     2581 gattcataag cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt
     2641 ggcgtatgtt catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgt
     2701 ttgaatcttt ttttggattc ctgataactt ggataaatca aacctgattc ttcttttgct
     2761 tttgaataca gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg cttacgaaac
     2821 gtcacaatca atccttttgt cttatcagga tacgtacgat tcgtacgtga aaaagcttgg
     2881 atcaatcctg catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca
     2941 aaacctgtta atagacgatc cactacaatc gctaaatcga tttgtttccc aaattgtttg
     3001 aattccgctc tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct
     3061 gctaatgacc aggaagtatc ataatactga ttatattcct caataatttt ttgcatttct
     3121 tcttgttggg cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt
     3181 ggaaaatcag gatcctccat cgttcgtcct tcacgaatga gctgattggg aaactctttt
     3241 gttaaccaat caggctcttt cgtcatctct ttgatggccc gataatactt tttggccatg
     3301 tcaatggaac tcgtggttaa gattgcagat ttcgttggcc tgccatttcg aaaatcaaat
     3361 tttgtatagg cattatctgg acggaacatt ttatgaatga ctttttggat atgttcatct
     3421 gcttcaaaaa cagctgggtc caaataactt tcttttttga caggttccat ttgatcaatc
     3481 atacgattga tctgttctga tgaatactct gcatatgttt ctacttcttt taatttccga
     3541 tagattttat tctctagtga agtcggctcc acggtgtttt catgctctac ttgaaatcct
     3601 aagacagagc catcttctag cgcattctta atggtatagg tatgcaagac ctctccatac
     3661 tgatcatggg ttgtacgtgc caattgccct ttggcttgtt tccgattctc aggaaaaatc
     3721 ggtgtacctg taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata
     3781 ttttcagcac ttaacgctcg atgacactca tctactacaa acacgatatg ttggcccatt
     3841 aatttttgga agcgattggt tccttttttc tcttcttgtt tctttgcata tttcaacgcc
     3901 gcatctagtt tttgacgggt tgtaatgatt acaacatttg cattggcatc tgccaacagg
     3961 gtttcactta attctttggc acttcccgtt ccaacgatca agctattcgc tttggcgttc
     4021 ccggaagaaa tcccagtatt aaattccgaa gcaaattttg taaactctgt ggtggtttga
     4081 ttatctaaat ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct
     4141 aaaagcttag tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca
     4201 taccctgatt gatgtttatt tgcggcaata aacaaggctt gaatcgcatg tacttgataa
     4261 ggatgtaaca ccatgagggt tttattgtct tgatcttcac tgacgattgt ataatccgca
     4321 atcaaacgat gagcatccgg gatatttaac acttgtttac aaaactcata taagttttct
     4381 actttttcat tgtctttcgt tcgccaacta aataaaaact tagggtgcaa gtctttcggt
     4441 aaggcatttg caaaataccg agtggtttgt tcattggaaa ccacaaacaa ctgtaaggtc
     4501 gaaaagatat tgttccgaaa cataccttct tctgcatatt ttttgatttg gttaaatgcc
     4561 tgataaacac catcttttgc agttacttgt ttcagctcga tttggacaat cggtaatcca
     4621 ttgattaata aggtgacgtc aaaacgtcga tcccgtgctt ctatgttact tccacgtttt
     4681 gcaatttgat ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa
     4741 aggataagtg agacagaacc caattgcgaa tcttcccgtt caatcgtgat ccgagccatc
     4801 ccattttcac cttttaacca tttcgcagca tcaaatgggg ttttcgttcg cagcagtaat
     4861 tccgttttga tcgtttcaaa ttctttatcg gtcaaaggaa aatcattcag ttctgcttga
     4921 ttatttgaga taattttttg gcgtaaattg acccaaaggt cttcttctga ttttagatcc
     4981 ggacgatacg tccactgatt atgcccttct ccaagtacac gaatcaactg ttcttcgact
     5041 tctgcttcct cacggtgact aattttcatt cttcttccct cctagacaaa catcttttgt
     5101 aagaagcttt ttttcgtttc ttttagtaaa tctaacttac gttgatgaag agcgatcgta
     5161 tcatctagct gtttgaagaa agtaccgatt ttttgttgtt catcctttac aggaacctta
     5221 acactgatat tctcaatatc agaactgtta taagaaggca atgcccctac ttgggagtat
     5281 cttggcaaat ttatagtttc aaacaatatt ttaccaaaat agatatccca agtattatta
     5341 aataaataag ccatggtatt gttatctatt aagaaagggc ttgtgaccaa acgttttcta
     5401 ttaagcatta ttgctgcgcc aactttagca aatataatcg ctggcacatc ttttattggc
     5461 ttccattttt tacgctcaat ttgctcatta gatacataat gatttgcatt tctcatttca
     5521 tactcattac caatgttatt catgtcggaa actttaaaaa atggagtacc ttcagaacca
     5581 ccttgctcgg catcaggaaa tccaattccc gattgagttt tacctaattc tcccaactta
     5641 cgctcttccc aatcttccgt aaaaccagga aaccggattt ctggcacttt tgctccgttt
     5701 tcagggaaca ttttttgtaa gaatcctttt ttcgtttcct tcagcaaatc taacttacgc
     5761 tgatgaagag cgatcgtgtc gtcaagttgt ttgaagaagg tgccgatttt ttgttgttct
     5821 tcctcactag gaaacattgc tactatattt ttaaactttt ttagagacag aacatgttga
     5881 cctgttccct ctgcttgaat agataattgt cgttggattc cattgttaat acgtcttaag
     5941 aagaagtcgg aatcagcaga ttttaaagta aatacaggat aaaaatagct tataatccct
     6001 ctatcaataa tgtcattacg gttaaacaca aacacatcat tatcacttcg actacggaac
     6061 gtgaaatatc ctctaggtag tacaaagtaa ccaatattat tttctgtagt tacttgccga
     6121 tttgcaaaat agtcttcttg caaaactatc cctttttgtt gagatgaagt tagcatagga
     6181 aattgatttt gtacacttgt cttttcaaca taatctacaa cgaagtcccc caacttcctc
     6241 tgttcccaat catctgtaaa tcctgggaaa cggatttctg gttgtgtatc attgctcatg
     6301 tttgaacacc tccagcgcac cttgtaacca cgcttcattt tcaggtgttg tttgcaggga
     6361 agagatagct tctaaaagtt ttttttctag cacttgctgt tcttgtcgta tcttcttgat
     6421 gtcatttccg acatgaacca tatcaattgg ttcttcttct tcgaaagtat ctacatagcg
     6481 tgggatattt aagttatagt cattttcttt gatttcatca aaggttgcca aatgagcgta
     6541 tttctcgaca tcttttcttt cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga
     6601 tagtttattt tgatttttcc ctttaacaaa ttcacgacta gcatcaatga ataatacatc
     6661 tcgagtttga cgattcttct tcaacacaat caccgtcgtt ggaatcgatg tcccaaagaa
     6721 caagttcgca ggcataccga tcacagcata aatactgcca tcttctagta atttttgacg
     6781 gatgacccct tctgcagcgc cacggaataa aactccgtgt ggtaagacga tggccatggt
     6841 acctgtttcc ttcaaatgat agaacccatg aagaagaaac gcaaaatctg ctttggattt
     6901 tggcgctaat ttcccataac gattaaaccg agaatcatct aagaaagtcg tatctgctga
     6961 ccaattagcc gaataaggtg ggttcattac tactgcatca aatgtgtaag gttcatctgt
     7021 tggccagtcc ttattcaacg tatccccatt gcggagattc atttcttcgg cgtcgacacc
     7081 atgtaaaatc aagttcattt ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg
     7141 atatttaacg ttatctgggt gtgttaagta gttacgtacg tttaacatca atgaacctga
     7201 acccatcgtt ggatcaaaaa cactaaagaa tcgtcgttcc ttttgatcga gcgttacgat
     7261 ttgcgccatc atatcagaaa ccatatgagg tgtgtaaaat tctccggctt tcttccctgc
     7321 ttctgaagca aattgactga tcagaaactc atacgcatcc ccgatcacgt ccccatcgtg
     7381 ttctaaaaca tctacatcat tcaatttctt gatgacttcg gtaatcgtca catttcgttg
     7441 ttgctcatct gttcctagtt tttttgattg gagatccaca tcatcaaata acccattaaa
     7501 ttgattgtag gtagatgcca attgcacaaa ggctttgttt agatcgttca attggaacgt
     7561 tgcttgtttt gcttgatccg ctaagacgtt gaaaagatac tctggtgcaa ttgcatatcc
     7621 caaaatatca acgattgtgg caatcagatc ttcttttgat tcttcatctg aaagtaattc
     7681 acgatacaac attgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa
     7741 aagaaccact tgttccaaca atcgatcgga taaatattta tagaaaatca gtcccaataa
     7801 ataattttta tattctgacg catccatttt actacggaga ttatctgccg cactaaataa
     7861 tttttgattt aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat
     7921 ataccctctt attttagcat accccctttt ataaaaagca aaaaaaaaaa gaaagaaact
     7981 tattcttgct aataaactac gcgaaaggag tctaacgttg tttgatataa cttagactga
     8041 tcgttaactt attttttata tttctacata gttatcttta gaaccatttc agatacatat
     8101 gaaacaatta catatgattt tttcataaaa aaacagtact gtctagtagt ataaaatata
     8161 caaatttctt aaaaattttt tcactaaaat cactacctga aacagtctaa aacacttata
     8221 tatcaacgtt taatgaaact atctccgttt catttcagat acaaataaat atagtcgggc
     8281 tccaaatccg aaaaaaaata cccccagtag cttatcctaa gctactggag atgtataagt
     8341 acggtcatat aacaacgggg tagggtaaat aaatgaaaaa gatggaagaa tcattattca
     8401 ataacttttt taaatacttc gttgtaatca gttacctgtc cgttcttaaa taccacagaa
     8461 taatcttttt tcccattttc aagtaaccga agtggaatgt ctttaccaac ttgctctgtc
     8521 actgcattac tgatacctgt tagattagct aataaatctg ataaagattg agcatctgta
     8581 gaaatctctg tatcagtatt tatcgtgatt cctttatctt catttacaag cttccaagta
     8641 ggtggtatag ctgtgtatgt ttcgatgaat gaatcaattc tttcatctat cgtcttccca
     8701 gtatctactt ctgtctcatt actcttagat gaatcgattc tttcatccat cgtctgctcc
     8761 gtaactgctt ctgtctcatt attctcaccg gaaactctct tcacacccaa acttatatat
     8821 tgtccatctt tagtgtgcat agttaaaatt cccacgcttt tactaataaa ttcactataa
     8881 ccagttcttt caatagagac ttcaatgtat ttcacactat catactcaaa agaaaaatag
     8941 acatgtaaaa gcattttgtt gtttacatct acataataat cctttgactc tacttgagac
     9001 cttcctatac ggaatacagc tttactgtct gatatctcta taccagcatc cttattatta
     9061 atctgccaat ctccaagtag ctcttctggc aagctagtag tttcttgtga acactttacc
     9121 acttcactgg tagaggtcga aacattcacc tccgcactgt tagaagaatt ttccaaggaa
     9181 tcaggagcct tagcacaacc aacaagtctt tatatattgt gcagccgata ttttcttcat
     9241 tcagtacacc cttgaaatat gcagtgttag ataaagcagt tcgtcctgat ttgctgaggc
     9301 acccatctct tcttgaacgt actccttgat tttcaaggcc gcccgatact ctttcggaaa
     9361 acgctgcttc actgaagcca gcaaaaaatc atccttctcc ggcaactctt ccttcttaat
     9421 cattcgggca ataaaaaact gtaaatgcgt cacaaatctt aagtaaagca tcgaatcttt
     9481 atcaaccttt ttttcgggaa acagatatcc tagcatagcc aagatatcct ttagtgctct
     9541 taccgagagc atggtttctt ccatatttgc ttgttcactt tgggcattta caatgtgaaa
     9601 cgcaatgttt ccagcctcct cttctggcaa ctgaacattc atttttttgt tgatcaattg
     9661 gatagcatag cagccgatct caaattcttt tgggtaatac ttttgaattt caatcagcag
     9721 gcgattttga aaaacaatgc ctttttgact gcgttcaatg gcatgcttca agtgatcaac
     9781 caaggtaaaa aatagacttt tattcagctt aaagtcaaac ttggcgttgg cataatcgat
     9841 aattcgcttg acgcaaataa ttagatcatc cggagcagcc tcaaacagtt taatgtaatc
     9901 cttttttaga gcatcagaaa gtgaatctaa tttaaatact ttttggattt tgtcagccgg
     9961 aaccaaatcg cctttcttta gttgaaatcc aatccctttc cccatcaaaa ttacttcttt
    10021 gccttgctta tctaagcaaa gaattaaact gttgtttaac gttttaatta ctctcattag
    10081 attcaccgtt ctctctaagc taagccattt cttgcgatta ctttttgata ccaaaagaag
    10141 ctttcttttt ttattcgttt taattcttta agattctcat cggttcggtc aacatatata
    10201 aaaccataac gcttattgaa gccttgatgg gtactaacaa catcaatcgc tgaccaaggt
    10261 aaataaccaa aaacatctac accatctgtt aaagctgctt gaatagccag aatatgtttg
    10321 gctagataat caattcgatc ctggtcatta attcgtctat cttcctcaac tatgtcgggt
    10381 gccccatagc cattttcagt aattaaaata ggtaaatcat agcgatcgta caatttcctc
    10441 aatgttaatc gtagtccagt agggtcaact acccagccat atggcgtttt atctacataa
    10501 ggattttcag ctgcccggta gacaccttct tctcccaaca tgatctgttg atcccccgct
    10561 ctagcggaaa catctgatcc gtcatttttg ctggcagcga tggtggcagt tgaataataa
    10621 ttaatcgcaa taaaatcggg tttaccggag gataaaattg cctgatcctc tgcctcaatt
    10681 ttaggatata atccacgatc attcaaatag ttttcaaaca aataattgta ttttccccga
    10741 actgctacat ctaagaaaga ccagcctctc aatgtttccc aattatgggc tgcaattgca
    10801 tcttctggac tggaggtagc ttgatacata gccgttaaat tgattgctgg accgattttt
    10861 cctttaagat tcaactgatg atacaagtga attactctag cttgagctaa taaaacatgg
    10921 tgattaattt gaaagagttc tttttttgaa ggcagctctc cacttggtgg caaaccaatc
    10981 gctccaggat gaaggatcat cgtgttttgc tcattaattg tcagccaata gttcaccttg
    11041 tcaccatact cagtaagcaa tacctgtaca tagtccacaa aataatcgat agttgctcga
    11101 tttaaccaac cgccctgctc ctctagaaaa taaggtaaat cgaaatggta catggtgaca
    11161 atcggttcga ttccctgatt caggcattca tcaattactt gatgataaaa agaaattccc
    11221 ttttgattgg ttttattatt ttcaatatcc ataattcgcg tccaagcgat ggaaaagcga
    11281 aaagctttta agcctaattc tttaaacagc tgtatatctt ctttatagtg atggtaaaaa
    11341 tccgaggcaa cttcaaaatt agctacccct tctgtatgct gatacttgtc aataatagac
    11401 ggttgtttcc catcctcctg ataggcaccc tctgcctggt aggcactaat ggaggcaccc
    11461 cataaaaact tctctggaaa tgctgaaagc tgtttgtatt gcacaaaaat cctctcctta
    11521 actaatttgc atcaatggtt cgtgttcctt taagcttccg cttgcttcac taattcctgc
    11581 gtaattttca ctgttggtca ctaccacaat cactgtatca tcgtagccag cgtttctaat
    11641 ggcagcgaaa tcggcgttga ttaataattg tcctgctgac acctgatctc ctgcttttac
    11701 aaatacttca aagggttccc cttttaaatt cacggtgtcg atccccacat ggatcaataa
    11761 ttcaatacct tctttagaac gtagaccaat cgcatgttta gattctaaaa tagtaatgac
    11821 ttctccctca acaggtgaat aaatgctgtt ttcttctgga tgaatggcta cgccctttcc
    11881 caatagtccc ttagaaaaga cagcatcgtt cacttcagaa ataggacaca cggttccttt
    11941 taccggattt tcaacaatga ctgcttcggc tattgccgtt tgctctgatt catcagaagt
    12001 atcgtcaaat ccaattagat aagcaatgat aaaggttgca acaaaggcga ttacacacgt
    12061 aataattgcg tgaacaatat tcattggatt ttctccaata aaggctggta aggctgctaa
    12121 gccaggggaa acaaaggcat atctgactaa accgctcaag ccagcataca aaccagcaat
    12181 tgcaccacca atcatggaag caactaatgg ttttttcagc ttcaaggtta ctccatagag
    12241 tgaaggctca gtaattccca ttaaagcagt gatcccagtg gacacagcta gctgcttcaa
    12301 ctctttgttt ttagttttcg cggctacagc aaaggtagca cctgattgag caatattaga
    12361 agccagcatt cctggcccat tgatcatttc agaacccgta ctgctcaatt gcatagtagc
    12421 gattggcgtc attgcccaag ctgtacccgt aaccaccaaa aatggctgca aaccacccat
    12481 caaaaatgga attaaccaag ctgctttgcc gttaatccat tcagccccca ttgctataag
    12541 atcgttcagc aacgttccaa acggtccaat gacaaccaac gctaaaggcc ccataattaa
    12601 aaataccagc atcggcttag taaagaattt aataaacgaa ggcgaaattc gatccgcaaa
    12661 gggctctaca taggacatga tccaaacagt aaagataatc ggtaaaactg atcccgcata
    12721 atctgcagct aataccggaa ttcctagaaa agaaacggca tcgccagtag aaaaaagctg
    12781 tgaaatattt ggatgaagca aaatgccagc aacagtaatc gccaaaattg ggttgacatc
    12841 aaactttatg gctgccccat aggctaatag tactggcata aaataaaagg ctgcatcagc
    12901 aatagtattc aaaatgatgt aagtttgact actgtctgat agtacaccag tcaaagtcaa
    12961 gacggccaaa atggctttta ccatcccggc acctgttata gctggaatca tcggtgtaaa
    13021 ggtggtagaa atgacactaa tcaatcttga aataatactt tcttgattgt ctgaagaggg
    13081 gcccttttta attgaagttc ccacttcctt ttgaatttcc ttgaaggctg tttggacttc
    13141 attgccaatc accacttggt attgtccacc gctatccacc acactaatta caccaagtgt
    13201 tttttctaag gctttcgtat cggctttcct tttatctttg agttcaaaac gcagtctggt
    13261 ggcacaatgg gttaagctga ccacattttc ttttccccca acctgttgca atacatttct
    13321 tcctacttct ctgtaatcca ttattctctc tccttaaagc agtaaaaaag accaagtcaa
    13381 accacgcccc aatcaacatt gaagtgttag tttgtcttgg tcttgcctgc ataatcagta
    13441 acatgccaat aacgttattt tgctgtaatc caataatagc acattatgat agcgcaatca
    13501 acagggaatt taatttttta tcttttcgtt tgaccgtata gggtaatata acagttagat
    13561 cacatcaact agggtatatc tgcgtatact tactgctaaa ataagtatat ctaatattaa
    13621 ctataccagt aaaaccattc ctacataacc agcgggaaat tcccaaaatc gtgaaagcat
    13681 caaaaatgca tataacggat cacacaactt caacaattct gctcttggga aaaattgatg
    13741 aatttggtga gtaaaggatg gaataaacag cattccaaca atataggcaa caatagtgat
    13801 acttgttttt ttggtaaaaa tcagtaagca atagccaatt tcaatcattc cgagatataa
    13861 aggtatcgat agaatgattt gggctagtat tcttaaaaat taactagcaa atgtttaaac
    13921 tgatttattt ctaatctaca tgtagtaagt atttcaattc caccaattat caataaaaat
    13981 accaaacata tccataataa ttttttcttt ttcatttctt ttatattgtt ataacagtga
    14041 ataacataaa aattcccgac aatcattgta atattttttt gtcacctgta ttagtattta
    14101 atttatagga aactataaaa actccctagt gaatttacac ctttgggagt tcctgtttgg
    14161 acggtatgca taatacattt aaatcaattt aacattaaca cctcagcata tggtatctcc
    14221 acatatatac ggtctttctt tggatagtac tcaatattta tgttgatagc acttccattg
    14281 tctaatatag ctttagcccg gtatgtgtta tctccatttt catgagtaat cgtcacatca
    14341 tcacatgatc gatttaagtt gtactgttct tgtaaaatct gtgttaccaa tggttttgat
    14401 gacttctcta atccagatgt acctgtcatt tttgaaaaaa taatacctat aatagccaag
    14461 acaacaatac ctataatgac aaacttcttg ttcatattta ctttattcaa ctttttatct
    14521 cctatctcca aaataatttg ctcttatccc tcttatcagt acattgacat ccctaaaagt
    14581 acaccaaaga ttgctccagc tacccctgct atcatcaaaa tcgtacccat tttctcatct
    14641 ttttctcgat ataagtagcc cataataaca gcaacagcac caaatacaac aggaataaat
    14701 gctaacgata ctaccataga aatccaacca ataatcaaat acagcttccc tgtctcttgt
    14761 ccatgtacga tttgttcctt catttcatta attgtctgct cagtactttt ctttactgtg
    14821 tctgttccgg ttgtctcatc tgtttcttgt agtgctccac aaaactggca atacttactt
    14881 tcatctgtta tttctttgcc acattttcta caatacatgt cttctcctcc taaaatcatt
    14941 caattgctta ttagttaact gtttacttta ttaaatacat cagaaaacag ctcattcatt
    15001 ggtacactta atatctcaga aagcatcttc atttgatagg gtttaggaat cgttctccct
    15061 gtttgccacg aacttacaca ttgctgtgta acattcattt tttttgctaa ctccgtttga
    15121 gaaagtcctt tttcttttct gtatcggctg agattactta cttttttcat gattctcccc
    15181 tcctgtttta ttatatacaa caatatgatg tatttcaact cataaaatat aaaaaacttc
    15241 aaataccatt acaaatacaa caacttgttg tactatactg agatgggggt gtattaatga
    15301 aacagaaaca atatgcaaag gtcattagag aaaagcgaaa agctctcggg tggacacaaa
    15361 aagaattagc tgaaaaaatc ttctctacac aacaggcagt agcaagatgg gaaaattctg
    15421 taacagagcc taacctcgat agcttaacag ctctttctag agccctagga acccctgtaa
    15481 gccattttct agacaatgtt gtcgtagaat tatgaggaag agtttttggc tctctatcgt
    15541 tctcttagca ctgaagatgc agttcgaaca attgactata tgaagctatt aaaaaggcaa
    15601 gaaaatgaac gcaatcagct attgaaagat tgaaaaataa ataacccacc gaactttaac
    15661 ggtgggtgta ttgagatgaa ataagattaa gagctatgga taacctgtct agtttaatac
    15721 aattgggtct tttctcttcc ttactctccg tccatcatct cttgttacac ttgtaaattg
    15781 atacagagga actatcccag ctcctgataa tatcaattct tttattgttt catctatccc
    15841 tgcccctttc gctaaatata ttaaaaaact actacttgct ttcacattgt atagtttctt
    15901 aatatcctta gaagctcgtt caagagtgaa tccttgttta acaaagtagc ctctgttttt
    15961 tattaaagca tttttcacgc tgttatcccc aacattacgt aggcgttcac aaacagtgtt
    16021 aataagttct ttctctgtca ttagatatgt cccttgccac gataagccct gtaaaacttc
    16081 atgtgcttca tctaaaatca attgtttttc cttgctgagt tctgatcggg aattttgagg
    16141 aaatactctt ttagcctctt tttcgttaaa acatatctta acccattcat aagaaaaata
    16201 ttgatatgta aaatgaattt tttccagaag tggaaggata tcttcattca atttttgaat
    16261 cacagaagtt gcattaaaaa cgtctaatcg atagacattg cttctcttat gattaaagtt
    16321 tcctaacgct ttctcttcct ttaacctatt taggtattcg agcatcttca aaggaacatc
    16381 ttcatcatct aattttttta tcaatcctat aaaagtcata agtgatagta acttattaca
    16441 tttatctatg tcataagttt taccatagta agaactcatt tttttagcta attgttcccc
    16501 cgacaaaata caagtcacac tttccttact atcatcaaaa aaattaacat cagaaagcaa
    16561 gacatcatag acacgtcgca tttcctctag ataattcctt attgtcctga aaagtttgtc
    16621 tttcattgat tcaagagtgt caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg
    16681 tgtaggatac aagtctaggt tctctaatag ccaattaaat gtttctaccc gactgtcttt
    16741 ctttaaaatt agcatcacaa ctccaataaa attttcactg atatcacaat tactggaatg
    16801 acaataaaaa atggaatgcc cagttttact ggtaaaaata ctagcagagg gattatgatc
    16861 gttgtgaaac agacatctta agtttttact tgacttaagt aatttattca tatcaatggt
    16921 taacaatctt tcataaatct cattaaaaga catgtcggag gaatcaaaca ggcagtcttt
    16981 aaaccactgt ttcatttctt ctttgtcgtc attttttatc aacttaacaa aatcggtttc
    17041 attgactagt tcatactctc tccctgaatc actttgacaa tttttagaac atgtcatggt
    17101 aaaatcaatc ggaagctcag taatatctaa catgttgtct tcattaatta ttatcactgc
    17161 atctgactta tatcctccaa aaaacattct attcgaagca tttacattgt catcacaccc
    17221 ggggatgttc tttttcaagt attcgtaaac agcaaccatt ttaccatagt cagtaattgc
    17281 ttcttttaat agaaagacaa tcttgtattt ttctagattt tcagagtacc gaatactttt
    17341 ttgtatcgca aacgcagaat tatgaactaa attgatagat aaaacctttt ctacgcttaa
    17401 gtatctacta tcatctttac tcaaatctac taccttcccc ttctcatctt taacagtgtt
    17461 atcgacatcc agaataatta ctctggtttc cacaaaactc tccttgtcat tgcctttatt
    17521 ctttgtcttg cttggaataa atgattgtga tgaatttagt atctctacaa actcatctct
    17581 tgtaacttct ctatagttat ctaaacacct atctcttaca tcagtttttt ctttttttgt
    17641 aactggcttc tctaataatt tttcttgatc tacataaatt ggaatattaa tcattgatgt
    17701 ttccatttgt gtttcctcct tataaaattc tgttaaagac taaaacatac agataataaa
    17761 aacaaactct ttattactat atgttttagt cttatttgtt tactcaacct ttataccgtt
    17821 ccatgaaact atcaatatca gattttttaa atctaatgac cgtatcaatt tttataacag
    17881 gtaacccgta ttgagaaaca tattttctaa tcgttcctac tgatacacct agatattcac
    17941 ttgcctgctg atagtccata tactctagag tgactccaga aataatgtct tctagcatag
    18001 gctttagcac tgtaattatc tctttcactt gttgttcaac ttgtatagaa acaatgctat
    18061 caatgatttg tagagacata tttccccctc ctttcgtgat tgcttgaaca aattttctaa
    18121 gaattatctt cgtatttttt gctcaaaata aaagtatttt gttattaacc gattaatcaa
    18181 acaaaaaatg attaccagtt catcgacata cacataatac ctcgataaaa aaggggtgtc
    18241 aacatctttg taaaatttat ttacaacacc ccttttttga ggtatactaa ttttaggagg
    18301 tgtggtcaat atgataaaac gtccaccaat aaattattta gaacgaaaaa agatactcgg
    18361 aacaaaaata aaagctataa gaaagagtaa aaaattaaca caaccagcat ttggtcttat
    18421 gattaacaac ggtcaattaa ttgacaaaaa aactatctac gaatgggaga agggaaccta
    18481 tttaccaata ccagagcgat tatcccgaat tgctgatttg ggaaatatga gtatagagga
    18541 gttagtttgt ggcaatgtag aagaatatat tttgggaatt attctttata gagatagcat
    18601 tgtactagat ggtataacat ttccagataa aaacctgttc cagcatcttc gtcaacagtt
    18661 cccaccagta cactctaatt tagatacatg gctggataga tattccaaat tagaaccaga
    18721 gatgcaagaa tttattgcta acaagacttg taataaagtt aaaaatgaaa aaattagtct
    18781 tttcaatatc ttaaaaatag aagaattatt catcaatgct attgtagaaa aatttgacaa
    18841 taacattctt ttcctcacta gcagtataga agaactacta gaaagaatgg tagatgaatg
    18901 gctacctatt caattgaaag acatgtctta tcccgaagaa gctgtgagag aaattactga
    18961 taatataaac aagctagaac aaacgatttc atcaattggg aaaaaataca ctaagaaaaa
    19021 aatgaaggga ggtgatacca tctagtggta agtactcctt gtaaactaag aggagatgat
    19081 ttaatggcaa aaatatcaaa gtacaaaaag aaaaacggtc aagtggcgtg gatgtataaa
    19141 ggacacattg ccaccgatcc acgaactggt gaaaaaataa atactacgag aagaggtttt
    19201 agtagcaaac aagaagctca aaaagattat gacgagtaca gacatcaaat attatacggt
    19261 gttaaaaaga aagcacctga tatgacattt gaagacctgt ataatgaatg gataactcat
    19321 cagcgtacca gtgttaaagc ttcaacaatc gcaatctcag taagatacgc aaataatcaa
    19381 atacttccag catttggtaa gcttaaacta tcgaatatct ctgtcccata ttgtcaaaaa
    19441 gtggtagatg aatggcacag taaatatgaa agttacgatt atatgagaaa acagactgca
    19501 caaattttac gctacggggt tgcaatgcaa tatattgaca ataatcctat ggaaaaaaca
    19561 cttcttccac gaaaaaaaga gtatgagaaa aatcgtaaat tttactcaaa agaggagctt
    19621 aataatttac tggatgcttt taaagatttt ggtaacatga aacaatacgc cttcttcaga
    19681 ttattggcat acacaggtat gagaaagagt gaagtactcg ctttacagtg gaaagacata
    19741 gacacgttta ataaagaact acatgtcaat aaaactcttg ctgtagatga atttggtaaa
    19801 gttataattc aatctccgaa aacaagagct tctagacgag ttatctcttt agatactgag
    19861 actctttcta tcctcaacaa ctggaaatta cagcaaaaag aagagtattt aaaacttgga
    19921 tacaatacta gctctaaaga gcaacatgtt tttacaacag ttaaaaacac tctatacatt
    19981 ccaaacactg ttaatgattg gttaagatat attttgaaaa aatataactt gcctcgtatt
    20041 acacctcatg ggtttagaca tacacatgct agtttattat tagaagcagg agaatctgtc
    20101 aaggtagttc aacaacgatt agggcatgaa aatagtaaag tgacattaga catctatgca
    20161 catattacaa ataatgcacc taagaaaaca ggtcaagact ttgctgatat gatggctcat
    20221 cagtaaaaga gctggtcaaa atctggtcat ttctagtata caggcaaaac aaaaaagcta
    20281 gaacccttat atatcaaggg ttctagctcc tataacaggt ttacccaaca ctgccttcca
    20341 tctcataact gatcaatcga ttgagttcga ctgcatattc cattggaagt tctttggtga
    20401 atggttcaac aaagcccatg acgatcattt cagttgcttc agattcggtc aggcctcggc
    20461 tcatcagata gtagagttgt tcttctgaga tttttgagac tttggcttcg tgttctaaag
    20521 agacttggct gttgtggatc tcattgaatg ggatcgtatc ggatttcgac cattcgtcca
    20581 tgatgatcgt gtcacattcg atatgagaga tcgaacccgc actgtttttg gcaaaggtta
    20641 cttgtcctcg atagtttact tctccgccgt cttttgaaat tgatttagag acgatagagc
    20701 tagatgtatt tggtgcattg tggatcattt ttgcacccgt atcttggatt tggtttgctc
    20761 ctgcaaaggc gatggagagc atggtgcctc gtgcaccttt tccatctaag taaacacttg
    20821 ggtatttcat cgttgttttt gcacctaggt ttccgtcgat ccattcaacg gttgcgcctt
    20881 cgtatgcttt tgctcgtttg gttactaagt tgtacacatt gtctgaccag ttttggatcg
    20941 ttgtgtaacg gcaatacgca tcttttcttg taaagatttc tacgatggcg gcatgcaggc
    21001 tgttacttga ataagtaggt gccgtacaac cttcgacata gtggatgctt gctccttcgt
    21061 caacaatgat cagcgtacgt tcgaattgcc ccgtgttttc cgcattgatc cggaagtacg
    21121 tttgtaatgg aacatcgact ttgacggctt ttggcacgta aataaaggtc ccacctgacc
    21181 atacggctga attcagcgct gctaatttgt tgtcggttgg cggtacaagt tttgaaaagt
    21241 actctttgaa taattcaggg tattctttca aagcagagtc agtgtcagta aagacgattc
    21301 ctaacttttc gaattcatct ttcatattat ggtacacgac ttctgattca tattgtgcgg
    21361 aagcaccggc tagataagct cgttcagctt ctggtatacc aatcttttca aatgtatctt
    21421 tgattttttc tggtacgtct tcccagtcac gagcgggttt gtcacttggt ttttggtaat
    21481 atttgatggc atcgaaatcg atgtctgaaa ggtcggggcc ccatgtttgc attggcattt
    21541 tgttaaatgt ttctagagat ttcagacgga attcaagcat ccattctggt tcgcctttga
    21601 ttcttgacat ctcacggatc acttcttctg tcaagccttc tcctgtactg aaaaccggtt
    21661 cgacatcatc gtgaaacccg aatttatatt cttctaattc tggtacgccc atgttctcac
    21721 tcctcttcct tgtggtaatg atggaaagct gcggtaccat cgttttccat tgcttttcct
    21781 agtgctttcc aggcaagtgt cgcacatttg atccgagcgg gaaatttagc tacaccacca
    21841 agcatagcag catcgcctaa ttggtcggga tctttgactt cttttccttg gacaagctgt
    21901 agaaagtcta cagttaaatg ttctgcttct tctattgatt ttccgatgac ggcgtctgtc
    21961 atcatcgagg cacttgccgt actgatggag cagccactgc cgtcaaaggc gatatttttg
    22021 atgattccgt cttttacatc gacttgcaat tcgatcacat ctccacatgt ggggttgttc
    22081 aattcgatct tgcttgtcga gtcagtcaag cttccacgat gatgcggatg ggaagaatga
    22141 tccaagatca cttgacggta taaattgtct aatttagata gtcccatgtt ggaaaaactc
    22201 ctttgttgcc atgattgctt caactaaacg gtcggcgtct tgcgttgtgt tgtagaaata
    22261 aaagctcgcg cgagcagtcg ctgatacttc aaggtacttc aatagcggtt gcgcacaatg
    22321 atgacctgct ctgacggcta ccccttccat gtctaaagct gtcgcgacat catggggatg
    22381 taacccatcc aaattgaatg caacgactcc tgtccgaaaa ttcgggtctt gtggaccata
    22441 aacagtcaat ccatcaattg cttgtaattt tggcaacaca taagcggcta gttcttcttc
    22501 atatcgatgg atattgtcca taccgagttt ttgcaaataa tcgattgcat ggcctaatgc
    22561 gatagcgccg gcgatatttg gtgtccctgc ttcgaatttc caaggtagtt ctttccatgt
    22621 actgtcgtat aattcgacaa aatcgatcat ttcgccacca aattcaacag gttccatttc
    22681 ttctaaccat tttcgtttac catataaaac accaatacct gttggtccgc acattttgtg
    22741 tccgctgaaa gcaaagaaat cacagtctag tgcttggaca tcaatggcca tgtgcggggc
    22801 agattgagca ccatctacta cgatgacccc gccgttttca tggacaagtt cagctaattc
    22861 ttgaacagga ttgaagacac ctaagacatt ggatacatga gcaatggaga cgattttggt
    22921 tttgtccgta atctttttcg ctgcatcctc catatccagg aagccttctt ctgttagttc
    22981 gatatatttc aagacagcac ctgttcgttt tgccaactgc tgccacggga taacattaga
    23041 atgatgttcc atgtaagaga gcactatctc gtctcctgct ttgatatagc ggtcgccgaa
    23101 ggttctggct acccaattca agcttgtcgt cgtaccacga gtaaacagca cttctgctgt
    23161 ttcttttgca tggatgaacg cccgtacttt ttctcttgct tgttcataat catgggtcgc
    23221 gcgttctgct aacgtatgga caccgcgatg aacattggcg ttatcgtgtt cgtaatagta
    23281 cgctaatgcc tggagtactt gttttggctt ttgggtcgtc gctgcattat ccagataaac
    23341 taaaggttca tcattgacgt cttgaaataa gataggaaaa tcattgcgta gctttttagc
    23401 atcgatcatc cattcaactt cccttcgatt acttcgacta attctttccg aacttgttct
    23461 acagggattg ctgttaagac agaaccgagg aatcctcgaa tcactaagcg ctctgcttct
    23521 tctttatgca atcctctact catcagatag tacatttctt ctggatcaac tcgtcctaca
    23581 ctggcagcat gtccagcagt tacttcgttt tcatcgatca aaagaatagg attcgcatca
    23641 ccacgtgctt tatcagaaag catcaagaca cgactttctt gttgggcatc tgcacctttt
    23701 gcccctttca agatgtggcc gataccatta aaggtcaatg ttcctttttc tcggatcact
    23761 ccgtgttgca aaatatgccc gatcgtatga ggggctttat tggttactcg tgtatcgatt
    23821 ccttggattt gtctgcccga gctgattgcc acgattttga cttctgcgtg tgcaccttct
    23881 cctgctaaat ctgagtcaaa gtcagcgatc acatgaccgt cattcatgac accaattgcc
    23941 cagtcgacag aagcatctcg taaaatgtgc ccacggcgat tcatataaga agtaatattt
    24001 tctcctaatt gatctacggc tgaatatttg atttttgaac cagctttagc gatgacttcc
    24061 acgatgatat tacctgatga ttttgctact tgttctttgg ttgtctggaa acgttccaga
    24121 taagaaaatt cactatgttc atctgccacg atcaacacat gcttgaagaa gtgctcatcg
    24181 gatgcgccat cttgaatgaa taaggactcg agtggttcat cgatcacgac atttttaggc
    24241 acatacagga aaataccgct gttcataaaa gctacatgga gtgcagtcaa ttggtcttcg
    24301 ttcatgttta ctgctttttt catataatat tcttggacta gttctggata ttcgatcatt
    24361 gcagtgaaca agtcggtaaa aatgactcct ttttctgcta attccacagg cagttgttca
    24421 aaaatcgtcc atgaaccttg ttggacaagc acaggattgt ctttcattgc atcaaatgcc
    24481 ggaatcgtgc ctgtttcact gatggtttgt gtttctttca catcaaataa cgaccagcga
    24541 tggaatttca cccgatcgat atgcggcaat ggcagttcat ctgctttcgc tagtgcgtct
    24601 tgacggaagg ttctcatcca atcaggttcg cctttactta aagaaaaatc atttaccgcg
    24661 tcaagataat cgagccagtt tttttctttc attcttgctc ccccttacgc ttcttcttct
    24721 ttatagtcga ttcctaattc tttactgata ccagcgtagc cttctgcttc cagacgttta
    24781 gctaggtcag cattccctgt catgactact cggccttcca tcatgatgtg aacgacatca
    24841 ggtgtgatat agttcaataa tcgttgatag tgggtgatga tcaatgcacc gaaattgtct
    24901 ccgcgcattt catttactcc tttagcaacg actttcaaag catcgatatc cagacctgaa
    24961 tcgatttcgt ctaaaattgc aaatgtaggt tctaacatca acaattgcag gatctcattc
    25021 cgtttctttt ctccgccaga aaaaccttcg ttcagataac gttcagccat ttcttctggc
    25081 atatttagta aagccatttt ttcatctaac tttttcaaaa atgccataac agaaattttg
    25141 tcgttttcat cacgtttcgc attgatcgct gcacgcatga attccgcatt cgtgatacct
    25201 ggtatctcac ttggatattg catagctaaa aataagccaa gtcgcgcacg ttcatctact
    25261 tccaagtcta aaacattttt cccgtcaaac aaaatttcgc cttctgttac ttcatagttt
    25321 ggattgccca tgatagcggc agataaggta gattttcctg tgccgttagg tcccatgatg
    25381 gcatggattt ctccggtttt cattgttagg tttacgcctt taaggatttc tttttcctcg
    25441 atcgacacat gtagattttt tatttctaag acagacatgc attatacccc caaattctct
    25501 ttcgttctca ctcattttag actaattgag aatgaagcgc aatgaccttc cttactaaaa
    25561 tcaaatatct tttatttatt attaaacaat tttgatttct aaattagaat aggtataaaa
    25621 aagatgagaa ataagccatt acagacttat ttctcatctt ttttgttcca taccttattt
    25681 tgcatcagcc tttactggtt taacagagcc gccccatttg tctgtgatcc attcttggac
    25741 ttctttgcgg tgaagcactt caatcagttt tttgattttg tcactgtctt catcaccttt
    25801 acgagtcgca ataatattca cgtaaggaga gttgtccttt tcaagcaata cggcatcttc
    25861 taatggattc aaccctgcgc cgtaagcgaa gtttgcgttg attgcaacta ggtcaccttc
    25921 atcattttgg tacgtagaag ctaaaagtga tgggtcgatc gtatggttga acttcaagtt
    25981 cttagggttt tcttcaatat catcgaatgt tgctgtttcc agatctactc catctttgac
    26041 tttgactaag ccagcatctt gtagaattgt gatgatgcgt ccccagtctg attcagaatt
    26101 tgaagtaatg attgttgcac catcttttaa ttcggaaaca tctttgactt ttttagagta
    26161 caagcccatt ggttcgatgt ggacagcacc ggcattgaca aagtcatatc ctttttcttt
    26221 gatttctttg ttcaaataag gaatatgctg gaagtaattg gcatcaatgt cgccttcttc
    26281 caaagattgg ttcggtaata catagtcatc gaatttgacg atttcaagat cgattccttc
    26341 atctttcaat aatggtttca catgttccaa aatctctgcg tgcggtgtcg gtgaagcgcc
    26401 gactttcaat gtcgtgtcgt ctgctttatt tgctgaatct ttgctgtcgc tgttgttacc
    26461 gcatgctgct aatcctacag tcagcaaaag cgctgctgca aatccaaaaa ttttcttttt
    26521 catatcctac atccccctat atcctattat cttttgtctg ttttcttagt taagaaatca
    26581 ccgattcctt gaacgataaa tacgattagt aaaataatta aagtcgcaac gaatgtcact
    26641 gttagattac cacgctggta gccttcctgc caagcaagac ctccaagtcc acctgcgccg
    26701 atcgcgccgg ccatcgcagt aaatccgatc atcgagatcg ttgtaactgt taatccagaa
    26761 atcaatgcgg gaatactttc aggtatcaag accttccaga tgatttccca gtaacttgct
    26821 cccattgcat ctgctgcttc cagtacacca gggctgactt cacgaaaagc aatctctact
    26881 agacgggcat aaaatggtgc tgcggataaa acaagagctg gaatggctgc ttgcgcacct
    26941 agcatagtgc ctacgattgc tttagtaaat ggaatgatca aaaccatcag gatcataaat
    27001 ggcgttgacc ggaaaatatt actgatgatc gatacgattc cataaatgat tcgagcatac
    27061 ggtttgtttt tgcgtccaat agagtaaagg atcaatccca aaactaatcc gatgatcgtc
    27121 actaagacca ttgagattaa tgtcatatat agtgtatcaa ggatagcctg attcatcgtt
    27181 tccgggttga tttgtttaaa atctagatat gtttctgaaa agcttttatc cacgatggat
    27241 cacctcaatt cctactcctt gttctttgat gaacacttct gcttgatcta attctttggt
    27301 ttcaccagtc atcaagacag tcaatgaacc aaaggaacct tctttcactt gtttgatctt
    27361 tccatacaca acgttgacat cgatgttata gcgtcgaatc gcttgggaaa tgactggctc
    27421 gttggcattt ctttcgctaa aggtcaaagt tgccaaacgg ccgcttggat tttctttgat
    27481 gagttcagct aatagttctg tcgaatcagc ttctggttcg atgtcttgtt gtacaaagcg
    27541 tttggttact tcttttttcg gttgtctgaa gacagtcaat acgtctcctt cttctacgac
    27601 tttccccaac tccatcacag cgactttgtt gcagattttg cggatcacat tcatttcatg
    27661 tgtgatcaag acgatggtca aattcaattt cttattgatc gctaacaata agtctaatac
    27721 ttcttctgtt gtttgcgggt ccaatgcgct agtagcctca tcacaaagca ggatctcagg
    27781 atcatttgcc aatgctcgtg cgataccgac gcgttgtttc tgtccgccgg aaagttgtga
    27841 aggatatgct tgtccgcgtc cttctaaccc gactaaacgc aacagttctt ctgcccgttc
    27901 tttacgtttg ctctttggta cgccagctaa ttccagcggc agctggatat tttcaagtac
    27961 agttcttgac cataacaggt tgaaatgctg gaagatcata ccgattttct ttcggaatgt
    28021 acgtaattct ttgtttttta attttgtaat atcttgatcg tttacgatca cttcgccgct
    28081 cgtgggaagc tcaagtccat tcaggagacg gactaatgta ctttttcctg cgccagaata
    28141 gccgacgatt ccataaatat cccctttttc aacgtttagt gaaacgtcat tgacggcttg
    28201 gatgtttccg tttttcccat tgaatgtctt tttgacattc gttagttgga ttaatgacat
    28261 gccattcaca tctttctctt atgatttttc aaacaaaaaa gccttcgccc tcttgcctta
    28321 agcaaaagga cgaaagcttg aacttccgtg ttaccacctt gatttaccgt tacttcgcaa
    28381 taacagcctc ttccagtact tggacttagc ttctttccgt atactgtcgc gctgtaacgg
    28441 gcactcccgt agtaagtttg cttacgctcg cttactctgc tctaagacca tcttccatct
    28501 cattttcgtt acccattttc agcaaacagg gctctcttca aacgtccgtg acatgtactc
    28561 ttcttttcaa agctttctcg tttcatatag gattgcttat gattatagca actggaagaa
    28621 tcagtgtcaa cttttttcaa gtgattttct gattttataa ttcgtcaaat tcttttggat
    28681 caacatcttt gaaacttaag atccacgcat tcatttcatc tgaatcattc aagatattga
    28741 tatcttcacc gattttatca ttaacagaag aaactgtacc cgttagcggg ctggcaaatt
    28801 cattgaccgc tttttccgct tcaacttcga tcagaggttc atcttttttt agcgattgtc
    28861 ctgcttttgg cagattggca aacgtgatgt ctccaagttc ttcttgcgct tcatttgtca
    28921 aaccaacaca gtattctttt ccattaaata agatccaaag gttgtccttt ttcttcaaat
    28981 gcttgcctgc cattttatat cagttctcct tcataaattt tttcattgaa tccaattgca
    29041 acaagctggt cctctcgtaa gatcagtggt cgcttgatca gcataccatc tgtcgataag
    29101 acttggctcg cttttgaagc cgtaaactcg cctacttgat ctttcagtcc aagttcccga
    29161 tatttcatcc cgcttgtgtt gaacaggcga cggattggta cttgactcgt ttctagccaa
    29221 gtctcaatcg ttgaggcctt cggtacttct ttgatcatat caacggtttg ataactaacg
    29281 ccatgctggt ctaaccacgc tttggctttt ttgcatgttg aacatttcgg gtaccagtaa
    29341 aaggtgtaca attttcttac ctccttgaag atcttgcagg tgcttcattt tggtatctca
    29401 tcgataatat caacccgata ccgatcatgt tccctaaaat cgaggatcct ccctgactaa
    29461 tgaaaggcaa tgggatccct gttaaaggaa gtaatccgat atttgctcca atattctcaa
    29521 agacatggaa cagcagcatc atgatcaaac cgcttgcgat ataggcgtaa aattcattat
    29581 tcgtatcgaa acacacccga atcatacgat agattaaaat aaaatacaag aaaatgacga
    29641 atgcaccacc gataaagccg aagttttcac cgataacggt aaaaatcata tcggactcac
    29701 gaacaggcac atagatattg cttacgttga atcctttacc aaacaagcca ccggtaccaa
    29761 ttgccaaaag tccttgggcc ggctgataac tcattcctga cgtatcatgg aaagggtcca
    29821 gccatgaatc aatacgtgca aactgataag atttgaatcc aactttataa agcaagtctc
    29881 ttccgccttc agtcgtaacc aagaagatcg ttcctgctcc aacgactact gccaagatta
    29941 ctacaggtgc gatgatgcgc caagagattc cagacatcag gaaaatcccg ccaaagatcg
    30001 caagaaagac aagcatcgtt ccaaagtcat cttgggccat gatcaatgct aaaaccggta
    30061 ttgtcacgat caagagcttc ccaatcagcc aaaagtccga tttcatcgtc cgttctttta
    30121 cttgcgtatt atgtttagtt acgatcagtg ccatcatcaa gatgtacgca atcttcatca
    30181 actctgctgg ctggaaagta aacgcaccga atcgaaacca gtttttagac cctgttgaag
    30241 cagctaaatt ctgatcatag aatttgagca agagtcccat taccactaac cccaaaccat
    30301 aaaggtaagg agtcagtttc cagatccatt tggaattcaa atgcatgatg acaacgattg
    30361 ctgccgctcc aactaaatac cagacaactt gcatccctac gcctttcaag actgctggat
    30421 tgttcggatc atggctaagt gctacatata atgagagcag accaatcaaa cataacagaa
    30481 agactggtaa aatcacgcca taatcaatac gattatcgtt cgaagctttt aatttttttt
    30541 ccatgtttca tcccttttct taaaaaccat actgctgatt attatagcgg tccagttaga
    30601 taatggaaag tctttccata gacatttggg aaaaattaac tttccgtttg tgcagttgct
    30661 tgagcaaatg cagtacaaaa gcctactgct tatctttagt gtatcataga actttttttg
    30721 atacatgcaa ttatctacca gctgagattc tcctcgctgt gagaagtttc tgtgctttca
    30781 ttgtatcgaa ggatctcttc ggcaatctct agcagctgat cagaaaccag attggttcgt
    30841 tggatgaaat aaaaatatcg atggaaattc aacggagtca ttggcacatt ttctggaaca
    30901 gagcgcttag acaaaatcga gcgaccgatt ccttccttca gcaacgcaac aattacatcg
    30961 ttacttttca cgatcatctt cttatcttgg atattcatct cttcaaaata acgctgcgtg
    31021 taatgaaaaa caccagagtt tgcttctctt acaagccaca atttgctttt tgggtcccca
    31081 gcgatgacta gctggtcttc catcaaagag tatctttgta cgccggaagt ctctaatggc
    31141 ttttcgataa aaccaaaatc ggattcatgt ttcgctactt gttctaatga ttcatgagaa
    31201 ttcgtgattt cgatctcaaa atgcacttta ggaaaccttt tgatcagatg tttcatcaac
    31261 tgaggcaaaa gatagacagc aaaggtgtgg gaagcaacga tccgacatac ttcttcttca
    31321 ttttgttcat tgagcaattt ttgctgagtt tcttcccatt cttctaacaa aaccagtgtc
    31381 cgttcatata ataagtcggc ttgcttagtt ggaagcatct cttttcttcc gttacgttga
    31441 aaaagatcga tattcaattc ccgctccaac tgtttgattt gattcgagac tgctggttga
    31501 gataaaaaca gtacttctgc tgctctggaa aaatttcttg tttcatatac tgctcgaaaa
    31561 gtcgttaata acttaaacat ggcaccactt ccataacttt ttatgataat tactataaaa
    31621 actatttgat ttaaatatac aaaaaaccat ctataataga aatgtacaac aaaacagttg
    31681 ctgatccaac tagtaactta gatgaagaca atcagctgtc tgcttgctcg taatcataca
    31741 ttcctattca ttttacaatg tattcgttca tcttggtact aaaatctatt aaataaaaag
    31801 gagaatatta tatatgaaga actttttgaa aattattccg atcccgatct gcgggttgat
    31861 ccttgggtta gcttcccttg gaaatctgtt taaagactat caccatgttg ctttaggcaa
    31921 tctaactggc ggtatctcta tgattcttat gatattcatt tttgttaaat tgtttatctt
    31981 attcgaacac acgaaacaaa cgttgcatga tccaatcatc gcctccgttt caccaacttt
    32041 tacgatgtca ctcatggtta tttgtactta ttttgtttca tatgaaagta ttgcaccaat
    32101 tgtcaaattc atttggctgt gcgctgttat cttccaagtg atccttgtct tttatttcaa
    32161 ttatcaccat gttatcaaag ctgatctttc aattgaagca atttatccaa gctggtttat
    32221 catttacgtt ggatttgggg tcatcactgt aactgccggg aacttcttcc cacttattgg
    32281 aaaaattttc ttttggattt cattgatctg ctatgcgttc ttattgccga tcatcatcaa
    32341 acgtgtcttt ttcgtaagaa atatggctca tccgacacgt ccgctgatta cgatcgttgc
    32401 agcacctggt tctctttgcc taacaggtta tcttaaaaac tttgctgaac ctaattttta
    32461 tcttgtcgta gcattgttcg ctttatctca agtattgtat ttcgttgtgc tgtctatgct
    32521 tccacgtttg ttcaagcttc gtttttatcc aagctatgca gcctttacat tcccgcttgt
    32581 tatctctgca acagctatat ttactactgt tcattacttc aacagtttag gtatctatag
    32641 tagcgtattg gatttcttaa aagttgcgga atgtctgttt gcaagtgtag ttgtcttcta
    32701 cgttcttgct cattacgtac gtttcttaat taaagaacat agcaaacatg gaaaatttgt
    32761 ttctaaaaaa atcgcttaat aatcgattca ataggttgtg aaaaagctgt tctgcatcaa
    32821 gtaataagaa cggccaaaca aaaacagctg ttcatgtttt tgtactggtt gggcttatta
    32881 ccgcagatgc aagcttttga acaccgttta ccaagaggtc gtaa
//
LOCUS       DAZKSU010000026        32426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000026 DAZKSU010000000
VERSION     DAZKSU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.026"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..522)
                     /locus_tag="ACG8Z3_001262"
     CDS             complement(52..522)
                     /locus_tag="ACG8Z3_001262"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0823590.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="ACG8Z3_001263"
     CDS             complement(681..1139)
                     /locus_tag="ACG8Z3_001263"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0823591.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="ACG8Z3_001264"
     CDS             complement(1310..2455)
                     /locus_tag="ACG8Z3_001264"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFQ0823592.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="ACG8Z3_001265"
     CDS             complement(2468..3385)
                     /locus_tag="ACG8Z3_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0823593.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="ACG8Z3_001266"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="ACG8Z3_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0823594.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="ACG8Z3_001267"
     CDS             4477..4647
                     /locus_tag="ACG8Z3_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823595.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8Z3_001268"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="ACG8Z3_001268"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0823596.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8Z3_001269"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="ACG8Z3_001269"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0823597.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8Z3_001270"
     CDS             6911..7792
                     /gene="dapA"
                     /locus_tag="ACG8Z3_001270"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0823598.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8Z3_001271"
     CDS             7789..8505
                     /gene="dapB"
                     /locus_tag="ACG8Z3_001271"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0823599.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8Z3_001272"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="ACG8Z3_001272"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0823600.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="ACG8Z3_001273"
     CDS             9222..10223
                     /locus_tag="ACG8Z3_001273"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0823601.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="ACG8Z3_001274"
     CDS             10220..11581
                     /locus_tag="ACG8Z3_001274"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0823602.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="ACG8Z3_001275"
     CDS             11541..12692
                     /locus_tag="ACG8Z3_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0823603.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="ACG8Z3_001276"
     CDS             12667..13836
                     /locus_tag="ACG8Z3_001276"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0823604.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="ACG8Z3_001277"
     CDS             14011..15390
                     /locus_tag="ACG8Z3_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0823605.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..16468
                     /locus_tag="ACG8Z3_001278"
                     /pseudo
     CDS             15671..16468
                     /locus_tag="ACG8Z3_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            16657..17325
                     /locus_tag="ACG8Z3_001279"
     CDS             16657..17325
                     /locus_tag="ACG8Z3_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0823606.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(17525..17845)
                     /locus_tag="ACG8Z3_001280"
     CDS             complement(17525..17845)
                     /locus_tag="ACG8Z3_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823607.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(17882..19264)
                     /locus_tag="ACG8Z3_001281"
     CDS             complement(17882..19264)
                     /locus_tag="ACG8Z3_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0823608.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(19420..20607)
                     /locus_tag="ACG8Z3_001282"
     CDS             complement(19420..20607)
                     /locus_tag="ACG8Z3_001282"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0823609.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(20614..21645)
                     /locus_tag="ACG8Z3_001283"
     CDS             complement(20614..21645)
                     /locus_tag="ACG8Z3_001283"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0823610.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(21661..23115)
                     /locus_tag="ACG8Z3_001284"
     CDS             complement(21661..23115)
                     /locus_tag="ACG8Z3_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0823611.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            23290..24195
                     /locus_tag="ACG8Z3_001285"
     CDS             23290..24195
                     /locus_tag="ACG8Z3_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0823612.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            24286..24963
                     /locus_tag="ACG8Z3_001286"
     CDS             24286..24963
                     /locus_tag="ACG8Z3_001286"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0823613.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            24960..25130
                     /locus_tag="ACG8Z3_001287"
     CDS             24960..25130
                     /locus_tag="ACG8Z3_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823614.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            25244..26140
                     /locus_tag="ACG8Z3_001288"
     CDS             25244..26140
                     /locus_tag="ACG8Z3_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0823615.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(26195..27565)
                     /locus_tag="ACG8Z3_001289"
     CDS             complement(26195..27565)
                     /locus_tag="ACG8Z3_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0823616.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            27803..28549
                     /locus_tag="ACG8Z3_001290"
     CDS             27803..28549
                     /locus_tag="ACG8Z3_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823617.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            28546..29385
                     /locus_tag="ACG8Z3_001291"
     CDS             28546..29385
                     /locus_tag="ACG8Z3_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0823618.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            29386..30336
                     /locus_tag="ACG8Z3_001292"
     CDS             29386..30336
                     /locus_tag="ACG8Z3_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0823619.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            30477..31229
                     /locus_tag="ACG8Z3_001293"
     CDS             30477..31229
                     /locus_tag="ACG8Z3_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823620.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            31248..32399
                     /gene="queG"
                     /locus_tag="ACG8Z3_001294"
     CDS             31248..32399
                     /gene="queG"
                     /locus_tag="ACG8Z3_001294"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0823621.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT        10579 a         5892 c         6708 g         9247 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatta
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
    15901 tggagcagta gccaaaaatc ggatccatcg tcgtttctac accagtatct gccatcagaa
    15961 aatcacgacg gatacaactg aatttaaata ccatgaagtg gatgccaaag gcattatccg
    16021 tcaaaagaaa ttgtatctag atccttttat ggatatgttc aatagtgaga ttttatccta
    16081 tcgtatctct gaaaagccca atgcattagc ggtaatggaa ggtttggagg aagctatcca
    16141 gaccactaac gattgtccct atcgtcgtac gtttcactca gatcaaggtt gggcgtatca
    16201 aatgaaagct tatagaaaca aattgaagga gtacaaaatc tttcaaagca tgtctcggaa
    16261 agggaactgc ttagataatt cacttatgga gaattttttt ggcttattaa aacaagagat
    16321 ttttcacggg aaagtttata actgtttcga ggaattaaaa tcagccatcg atagctatat
    16381 ctattactac aataatgaac ggataaaaca gaaattaaac tggcaaagtc tcgtccaatt
    16441 tcgtaaaaca accgtaacgg ttgtgtaaaa atatcatttt agagcataaa aaaaaggagt
    16501 ggatttctcc actccaaaaa agtctaactt ttgggggtca ctacagtgtc acagtccttt
    16561 ttttagtaaa aggatacatt tcttccttgt atattccatg cgtgttcgtg agaaagcata
    16621 tataatttaa ttaacagata gttaggggga gaaaaaatga ttttttcaat tacaatgaaa
    16681 ctggtgttcg ggttgatcgg tttattatta gtggtacgtt tgcttgggaa gaaatccatg
    16741 tcagaaatca caccgtttga tttagtctat acgcttgttt taggtgggat cttagaagaa
    16801 tcaacctatg atgacaatgt ccatgtaggg catgtcctat ttgccattgc gctttgggca
    16861 gtaatgattt atggtatcga acgtatcgtc caaaaaaatg aaaaggtcaa tcgttgggtc
    16921 aaaggcgagc catctgttct aatcaaagac ggcgtcatca acatgacaga gttgacaaat
    16981 aaccatatcg agatggagca gttgcgagca atcttaagac agcaagaatg cttttttctt
    17041 gaaaatgcca aacatgttat cctagagaat gccggtcaaa tgagtgtatt gaaaaaatca
    17101 gatgaagaca aagctttatc gatcctgctt gtagatgaag ggcaaattca gcataaagtg
    17161 cttcagtcca atcaattgac tgaagcatgg cttatggaga acttgaagaa agaaggctat
    17221 gcagatgtaa aacagctgat ttatgttgaa tggtcagaag aaaaagggct ctacatacta
    17281 aataaagaag acgtcatgca tgaatcttac cgaatagatg gatagataga cagaggttgt
    17341 gaaatcagcg ccttggcttg ttactcaaaa ctaagcgact ttttcacaac ctctgatatc
    17401 taatatacgt tgttccagaa gttgcttctt cgaaaaataa gccaaaattc cacaaaaatt
    17461 taagaagcaa ttttcgaaaa atcctttctt attttttgaa gttgatcact tctgtcacaa
    17521 cctctcatgt cacaaaccat ttttttgttt cctttaactc aatataccgt acgtcttccc
    17581 acaatatcgg ctgatggtca atcataaagt aatcttcata ggcttcacca atgaagtgcc
    17641 cttcgacctc atccaagagt cttccaaatt catcttttaa attgagctga atgattactg
    17701 ctttacgaga taaaaaggct tctgtcagta tactatcgat ttcttgcaat gtcatttgtt
    17761 gtttaggaag aggctgtttg cgtgcttctt cttcattttt ttgataccgt tgactagttc
    17821 ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat actcgttata
    17881 gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca ctcataccac
    17941 ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc aagagactat
    18001 gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca acaaaatcaa
    18061 ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt aaacctgtcg
    18121 cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag tggtaatgct
    18181 ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc gcttcgattt
    18241 tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa gagtatccta
    18301 cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg ccgacttggt
    18361 cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct ctatctagga
    18421 tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa aaacttcgat
    18481 cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg cttttcaagt
    18541 gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt ttcagacgtt
    18601 ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc cataactttt
    18661 ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt gagccattgt
    18721 ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg tagattccgg
    18781 tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt gttttaccaa
    18841 agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag acatgatggt
    18901 tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt tccatataat
    18961 aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga agatctctgg
    19021 cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa gccaagatta
    19081 atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact aatttagttg
    19141 taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat ttgcagtcaa
    19201 tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt tgattcgccc
    19261 acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt gtcaataaaa
    19321 aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga gaggttattt
    19381 ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa cagcttctgg
    19441 atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag catagccgac
    19501 aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt cttctttgac
    19561 aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta agacaccttc
    19621 ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt cataatcata
    19681 gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt tcccagcttc
    19741 taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc gagctctgcg
    19801 tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt catctaagtc
    19861 tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt cgcattcgct
    19921 acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat tcatgatgac
    19981 gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat ttgtatctaa
    20041 taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga tacctgaatt
    20101 gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca agcgatctgt
    20161 ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg aagcgctact
    20221 ggaaagtcct gcaccattag ggattgttcc ttccactaat aactcaaacc catgatcgat
    20281 tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg cccagtttgc
    20341 ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat cgtcaaagtt
    20401 tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt gcgttccata
    20461 agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac caatcaaatt
    20521 gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt cggcaaattt
    20581 tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt tgattttgaa
    20641 ttctgttact gtttcaaata ctgatccggc ttttaaatga atcgaaccaa atgctgagaa
    20701 gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat gtgccagttt
    20761 tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa ccacactaga
    20821 agcatctgta gcaatatcta cttggattct tttatcgggg ctagtcaagc gagcggcttc
    20881 tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa caagattttt
    20941 ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa aatcgaaagc
    21001 acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca aaggagcata
    21061 agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat ctcccgtcaa
    21121 gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg tgccgcgaga
    21181 ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat ccattgtacc
    21241 tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag cttcattttc
    21301 gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac caccatgcag
    21361 attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga gagaaaagcg
    21421 gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg gatctttttc
    21481 gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg attttaaact
    21541 gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt tctccaaggt
    21601 tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca agatcctttc
    21661 ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag ctggagccaa
    21721 tttgttttcc aatttctcaa cgatttgtgc atgttttttc tctgtcaatg taatcttcca
    21781 agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg cataagcttt
    21841 gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag tcatcccaga
    21901 agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt tatctagtaa
    21961 agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt gaccatattc
    22021 aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag ggaagtagaa
    22081 caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa agaaaaagta
    22141 agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga tgacgcgtgt
    22201 aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta gaccaacgat
    22261 ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata cggcagttgt
    22321 tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca actgatcatt
    22381 ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct gacggatcgc
    22441 agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg aaataacagc
    22501 agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat gagtaacaag
    22561 ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg cacctagaga
    22621 agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg tactcaaagc
    22681 tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca ataagatgaa
    22741 aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc caaagaaatt
    22801 ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta accatggttt
    22861 gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag gatcaaaaat
    22921 aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca tttttgcatt
    22981 cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa aataagtgct
    23041 taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca cacgttggat
    23101 cattttgctt ttcataatct gtattcctct cctgttcaat atataatact aagtaaaccg
    23161 gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat cgcttccttt
    23221 ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa ggagtaagga
    23281 gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt agaaaaagaa
    23341 ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt tggtcctgct
    23401 attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta tcatgtgaaa
    23461 aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg agactcgacg
    23521 ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt tggtgggagt
    23581 gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac gatggtctct
    23641 tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac ggaagaaacc
    23701 ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt ccttttaaaa
    23761 gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc tgttgaaaca
    23821 gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat cgcccagcat
    23881 ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg taccagtatc
    23941 aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc tctgacggat
    24001 gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt atttagccga
    24061 atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat gaaaaacgaa
    24121 aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca aaccgcagtt
    24181 tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga aacttgtggc
    24241 ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg tacacttatc
    24301 atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa agaagcttct
    24361 gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg aaaaatagat
    24421 atagagactg aactacagct cttggcccag catgatcgga tcattttcca gtttccgttt
    24481 tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt ggaacgagca
    24541 gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt ggcggaaaaa
    24601 gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag gccgtatcag
    24661 cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc acaatttatg
    24721 tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta tctctcattg
    24781 gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt acaggaaaat
    24841 cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc actgactgag
    24901 gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg aacttcatta
    24961 tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca gaatatcaag
    25021 accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa cgaacaatgc
    25081 agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga aattttctta
    25141 taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa gaatatgaag
    25201 cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg atgaaaatga
    25261 gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag ctcgtaagcg
    25321 gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag gagttgcggg
    25381 tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa cggaaacaac
    25441 gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa aagagacaca
    25501 aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa tcgtaaatga
    25561 ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc agacagaaat
    25621 tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa aagcacgtga
    25681 gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc aggcagcaga
    25741 aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta attatacgaa
    25801 tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc aagcagagtc
    25861 aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg tagaagcttc
    25921 tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta ataccaacag
    25981 cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa atcaaatagc
    26041 cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga gcatggagaa
    26101 ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat aaaaaagaac
    26161 gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt ttttcgcgta
    26221 caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat gagattctcg
    26281 tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt gtcacataat
    26341 tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct gcgtgcttct
    26401 ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca ggcaaaatac
    26461 ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt ggtagaagat
    26521 aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga taaaaaggcg
    26581 caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg gcaagtgtat
    26641 agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc gctgtaatca
    26701 cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa gtttgtacaa
    26761 aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg ataaaaatag
    26821 caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca atgatcgtct
    26881 ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct tgataggaag
    26941 caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag aatccaccat
    27001 ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca aataagatta
    27061 ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc gaaaaccaaa
    27121 attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga aacaagacga
    27181 caatgattcc gggaataatt gccggcaaat caggccacca ataattcata taagatccta
    27241 cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc caagcggtca
    27301 agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa ccagtcgaag
    27361 gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag aatcctgcaa
    27421 tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag gcacccataa
    27481 acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg gcactaagac
    27541 ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt caacaagaaa
    27601 aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat tgaactcttg
    27661 atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa actttttcat
    27721 cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa aagttaggta
    27781 aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag acctttcagc
    27841 cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc cacaaaaaat
    27901 taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat ctttgctaga
    27961 gttgatacca gctactactg gtaaagtcac cttttctgga cagtcggtaa actcagtccg
    28021 taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc caattgatgt
    28081 attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa gacctgggaa
    28141 aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag aatatgccaa
    28201 aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg caagagctct
    28261 agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg atgcgttaag
    28321 tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa cgattttgat
    28381 cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc ttttaaataa
    28441 acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc ttcaattggc
    28501 ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat ccaatccttt
    28561 attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc catgcttgta
    28621 ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc tttgatggga
    28681 gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt tggttttagt
    28741 tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg tttcgtgact
    28801 cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc cttttttgca
    28861 ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca tattttattt
    28921 gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt tagtggaatc
    28981 gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt cgatccgaca
    29041 atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat gttctttttg
    29101 acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat tgccatgttg
    29161 atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat gatcattaca
    29221 gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta tagttacaat
    29281 tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct tgctttttta
    29341 ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa aacaaaaaaa
    29401 tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg aaagcaggct
    29461 tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac caattcgatc
    29521 ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag tatcgtacca
    29581 gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa ggcaagcgat
    29641 gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg gttcgataac
    29701 ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag caaaaatgta
    29761 gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca tgcttggcta
    29821 gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc tgaaaaagat
    29881 gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa actaaaagaa
    29941 ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa attattagta
    30001 acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc agcttatata
    30061 tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat cattgatcag
    30121 ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc aagaagcatg
    30181 gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac tgattccata
    30241 gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa tattgaaacg
    30301 atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct agcttgagga
    30361 gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt tcgtcgtaca
    30421 gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt gtggctatga
    30481 attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg atcagccagc
    30541 aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta gctaaattat
    30601 tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata ggcaaatggt
    30661 tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt gtgttccttt
    30721 ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata accgtcaaag
    30781 catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag atccagtcgg
    30841 cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt tataatcgtc
    30901 tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca cagcaaaaaa
    30961 atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca tgggaagagg
    31021 taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt aatgcattag
    31081 ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca agaaaaaaat
    31141 ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg aaaaaagaaa
    31201 aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg acattaaaag
    31261 aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc accacggcgg
    31321 aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc catacttctg
    31381 gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt gatcagccga
    31441 aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa gtgcctaggg
    31501 atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat gatatactca
    31561 gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa tggcaaaaag
    31621 aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat gtcgctgtag
    31681 cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact gaagagttcg
    31741 gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct gataaaccag
    31801 gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca aaagcattgc
    31861 ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag acaaaaggga
    31921 tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt gatatctgcc
    31981 agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag atggaaccaa
    32041 aagtagacga aatatatcca aaattaaagc caatgctttc gatgtctaat aaagacttca
    32101 aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg cagagaaacg
    32161 cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt tcagaatgtt
    32221 taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga cgtctaggaa
    32281 caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa gaaacagaag
    32341 aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag ttaaaataaa
    32401 aaaatagtta acattattca aagttt
//
LOCUS       DAZKSU010000027        31311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000027 DAZKSU010000000
VERSION     DAZKSU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.027"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..903)
                     /locus_tag="ACG8Z3_001295"
                     /pseudo
     CDS             complement(296..903)
                     /locus_tag="ACG8Z3_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1426..2100
                     /locus_tag="ACG8Z3_001296"
                     /pseudo
     CDS             1426..2100
                     /locus_tag="ACG8Z3_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            2093..2254
                     /locus_tag="ACG8Z3_001297"
     CDS             2093..2254
                     /locus_tag="ACG8Z3_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823622.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2738..3334
                     /locus_tag="ACG8Z3_001298"
     CDS             2738..3334
                     /locus_tag="ACG8Z3_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFQ0823623.1"
                     /translation="MNPTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            3315..3761
                     /locus_tag="ACG8Z3_001299"
     CDS             3315..3761
                     /locus_tag="ACG8Z3_001299"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0823624.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            4104..4427
                     /gene="relB"
                     /locus_tag="ACG8Z3_001300"
     CDS             4104..4427
                     /gene="relB"
                     /locus_tag="ACG8Z3_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0823625.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLSFKIHARNSEAALIG
                     KYVYW"
     gene            4747..4974
                     /locus_tag="ACG8Z3_001301"
     CDS             4747..4974
                     /locus_tag="ACG8Z3_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0823626.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4990..6156
                     /gene="hemW"
                     /locus_tag="ACG8Z3_001302"
     CDS             4990..6156
                     /gene="hemW"
                     /locus_tag="ACG8Z3_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFQ0823627.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGNRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6391..7434
                     /gene="hrcA"
                     /locus_tag="ACG8Z3_001303"
     CDS             6391..7434
                     /gene="hrcA"
                     /locus_tag="ACG8Z3_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0823628.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7528..8091
                     /gene="grpE"
                     /locus_tag="ACG8Z3_001304"
     CDS             7528..8091
                     /gene="grpE"
                     /locus_tag="ACG8Z3_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0823629.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDIEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8142..9971
                     /gene="dnaK"
                     /locus_tag="ACG8Z3_001305"
     CDS             8142..9971
                     /gene="dnaK"
                     /locus_tag="ACG8Z3_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0823630.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10122..11288
                     /gene="dnaJ"
                     /locus_tag="ACG8Z3_001306"
     CDS             10122..11288
                     /gene="dnaJ"
                     /locus_tag="ACG8Z3_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0823631.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11656..12393)
                     /locus_tag="ACG8Z3_001307"
     CDS             complement(11656..12393)
                     /locus_tag="ACG8Z3_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823632.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12395..13897)
                     /locus_tag="ACG8Z3_001308"
     CDS             complement(12395..13897)
                     /locus_tag="ACG8Z3_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0823633.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14156..16150
                     /gene="uvrB"
                     /locus_tag="ACG8Z3_001309"
     CDS             14156..16150
                     /gene="uvrB"
                     /locus_tag="ACG8Z3_001309"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0823634.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            16160..18976
                     /gene="uvrA"
                     /locus_tag="ACG8Z3_001310"
     CDS             16160..18976
                     /gene="uvrA"
                     /locus_tag="ACG8Z3_001310"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0823635.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19239..20504
                     /locus_tag="ACG8Z3_001311"
     CDS             19239..20504
                     /locus_tag="ACG8Z3_001311"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0823636.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20508..21239
                     /locus_tag="ACG8Z3_001312"
     CDS             20508..21239
                     /locus_tag="ACG8Z3_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0823637.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21374..22258
                     /gene="rapZ"
                     /locus_tag="ACG8Z3_001313"
     CDS             21374..22258
                     /gene="rapZ"
                     /locus_tag="ACG8Z3_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0823638.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22255..23253
                     /gene="yvcK"
                     /locus_tag="ACG8Z3_001314"
     CDS             22255..23253
                     /gene="yvcK"
                     /locus_tag="ACG8Z3_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0823639.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23287..24222
                     /gene="whiA"
                     /locus_tag="ACG8Z3_001315"
     CDS             23287..24222
                     /gene="whiA"
                     /locus_tag="ACG8Z3_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0823640.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24790..25446
                     /locus_tag="ACG8Z3_001316"
     CDS             24790..25446
                     /locus_tag="ACG8Z3_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823641.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25625..26190
                     /locus_tag="ACG8Z3_001317"
                     /pseudo
     CDS             25625..26190
                     /locus_tag="ACG8Z3_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26889..27410)
                     /locus_tag="ACG8Z3_001318"
     CDS             complement(26889..27410)
                     /locus_tag="ACG8Z3_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0823642.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            27608..31288
                     /gene="nifJ"
                     /locus_tag="ACG8Z3_001319"
     CDS             27608..31288
                     /gene="nifJ"
                     /locus_tag="ACG8Z3_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0823643.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
BASE COUNT        10456 a         5248 c         6668 g         8939 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     2401 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     2461 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     2521 tgtttttaca taagaagact tcgggtattt tttagtttga ttagctctga tatacctata
     2581 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatatt
     2641 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     2701 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     2761 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctaatt taattagaaa
     2821 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     2881 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     2941 ttcagagaag tttaaatgtt ccaaacaggg tggaatgaga agatctcatc aaactaatac
     3001 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     3061 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     3121 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     3181 accaaaagaa cacacatatc tgggagaagt atatttagca ggacaaatat atactgttaa
     3241 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     3301 tcaagagcaa ttgattgaag ataaagatat ataaccagga agaaaatcaa actagacata
     3361 ttcgtaattt atctaatgct aaattagagg aagaagcaag aaaagttagt aattataata
     3421 aaaaaatggc tgcaaaatat gttaacgaag aggagttttg cttctcaaaa acaaaaatat
     3481 ataaacgtaa tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac
     3541 tttgccaaga aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata
     3601 tagaatattt gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca
     3661 attgtcatgc tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga
     3721 tcgttgaaaa aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa
     3781 taagctaatg tgagctatgc cttctatata agactaagtt ctattataag taacgaagta
     3841 attctcgttc cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac
     3901 atcctccagt aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa
     3961 aaagtagttg gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg
     4021 atcatttact acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt
     4081 tctaaaatca catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga
     4141 ttacggttcg tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg
     4201 ggattacttt atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca
     4261 atcgtcaaac ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat
     4321 tcatggaaga atccttaatg aatttggtgg gctggaatga cctgtcgttt aaaattcacg
     4381 cccgaaattc agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa
     4441 tcaattcgtt gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac
     4501 tgtttatttt tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat
     4561 ttgactagat ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg
     4621 taaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac
     4681 gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag
     4741 gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc
     4801 tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag
     4861 caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg
     4921 gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa
     4981 agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat
     5041 atcccatttt gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg
     5101 cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt
     5161 catcctacag cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct
     5221 gctaaacaac ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt
     5281 aatgagttta ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg
     5341 aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg
     5401 aaaaaaattg gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa
     5461 gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta
     5521 gaaggaaatc gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg
     5581 tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc
     5641 gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa
     5701 aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa
     5761 cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt
     5821 tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta
     5881 cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa
     5941 gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat
     6001 cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa
     6061 gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg
     6121 aacaatgtgt ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc
     6181 gaaacttgaa aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa
     6241 ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga
     6301 gtctttctct tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga
     6361 atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta
     6421 cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg
     6481 tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat
     6541 gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat
     6601 cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg
     6661 attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct
     6721 gagatattat ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac
     6781 cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc
     6841 gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt
     6901 caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg
     6961 acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg
     7021 acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt
     7081 tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc
     7141 aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg
     7201 gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg
     7261 agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta
     7321 gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa
     7381 ctagcagcta aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa
     7441 taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg
     7501 actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa
     7561 atgacagatg ctcagccaga accggaaatc gatatcgaag ctgcagaaga ttcgggtatt
     7621 tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg
     7681 gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat
     7741 gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca
     7801 attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg
     7861 aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt
     7921 gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact
     7981 gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac
     8041 aaattacatg atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga
     8101 tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta
     8161 ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca
     8221 tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa
     8281 tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa
     8341 tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac
     8401 cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag
     8461 gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc
     8521 aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac
     8581 caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag
     8641 tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct
     8701 ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa
     8761 tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca
     8821 aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg
     8881 tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt
     8941 tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta
     9001 caaaagtacc agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg
     9061 aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag
     9121 aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg
     9181 ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac
     9241 cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata
     9301 caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag
     9361 tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa
     9421 gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat
     9481 tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag
     9541 aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg
     9601 tgaaagatgc agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac
     9661 gtaatgatgt agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca
     9721 aagtggacga agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg
     9781 ttgaagcaaa caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc
     9841 aaaacttgac tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag
     9901 aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg
     9961 acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt
    10021 tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac
    10081 aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt
    10141 attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc
    10201 gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat
    10261 tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg
    10321 accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat
    10381 ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact
    10441 ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt
    10501 tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca
    10561 agtataatcg ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac
    10621 aaccaactac ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc
    10681 cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa
    10741 tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta
    10801 aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag
    10861 aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa
    10921 gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac
    10981 aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga
    11041 tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt
    11101 tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc
    11161 taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg
    11221 aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc
    11281 ggaagtagtt tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta
    11341 aaaacgctaa gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt
    11401 tttgtagttg tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat
    11461 agtttctgaa ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc
    11521 ttatgcgatg gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct
    11581 ggtacagtta agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcgaccggg
    11641 catttttggt ttttattaaa tatttaatac tttattcaag aaatcttttg tccgttcatt
    11701 ttgcggattt tcaaagattt gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat
    11761 gaacatgaca cgattggcca cttctttagc gaagcccatt tcgtgcgtaa cgataaccat
    11821 cgtcatacct tgttctgcta actttttcat gacgttcaag acatcaccga ccatttcggg
    11881 gtcgagtgca gatgtaggct catcaaaaag catgatgtca gggttcatcg ctagagcacg
    11941 agcaatagcc acacgctgct tttgtccacc ggaaagcatt tcaggataca tatctttttt
    12001 atcttctaga ccaactgtct gcagcaattt gattgctttt ttctctgctt cttctctaga
    12061 ttctttcttc aggtccatcg gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa
    12121 taggttaaag tgctggaata ccattccgat atgttgtctc accaagttga tatctgtgct
    12181 tttatccatc aagtgggcgc cgtcaataat aatttcaccg tcagaaggtt cttctaaacg
    12241 attcaagcaa cgcaaaaatg tactttttcc tgaacctgaa gggccaatga tacagacgac
    12301 atctccttcg ttgatggaca cattgatgtc gtttaatact gtgttatcgc cgtatttttt
    12361 gactaaatgc tcaactaaga ttttttctgc catcttactt caccttcttt tctaaaactt
    12421 ttgctaattt tgtcaatgct gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa
    12481 catacgtact ttgtgttgta cgtgcaacaa tgatcttacc agtttgcagc aattcgacta
    12541 caccgattgc ggaaatgatc gttgtgtctt ttaatgagat aacgaactga ttgacgaatg
    12601 atgggatcat gatgcgtatc gcttgcggca aaatgatttt ttgcattgtc cgattataag
    12661 aaagacctaa acttctggaa gcttccattt gtccgacagg aacggcgttg atcccgcctc
    12721 gaacaatctc tgcgatataa gcacttgcgt taagagtcaa ggtgataaca ccagcaagga
    12781 agtcaggaat cgtaaatcca acgataccag gcaacccaaa gaaaataaag aacgcaagaa
    12841 ccatcattgg aattcctcgg atgatatcga cataaatact agcaattgtg cgaagagcgc
    12901 gtattggtgc tacactgaat aatccaaaaa tcacaccgat tactatagcc aaagcaaagg
    12961 agatcaaagt taggagaatt gttctccaaa gtcctttcaa caatgcttga tagttgtttt
    13021 ggataagtcc aagaaatgtt gattcatcag cagtggaaga ctcactggaa gtttctgaac
    13081 catccgctac atattcttta ataatcttat cgtattcgcc tgttcttttt aattctttca
    13141 atccttcatt gaacatatta agaagctcag gattttgtcc ttttttaacg gcaaatgcat
    13201 attttccgcc agattctttt ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat
    13261 aaccgattac cggataatca tccatcattg catcgattgc gccaactttt aacccgtcgt
    13321 acaattgatc agcagcatca aaatatttga tagaatagcc atattcagat tcatggtcgc
    13381 ttaaaaattc agcactttcg gtaccaactt ttgctccgac tgtttttcct ttcaaatctt
    13441 cataagactt gatcgaagta tctccttttt tgacagctaa ttggatcccg ctatcaaagt
    13501 aagaatcaga aaaatcatag cttttctttc gttcatctgt tacagtcatt ccggcaatca
    13561 ttccgtctgc ttgtcctgat tccaaagctt gcaaagcagc agagaaacca cggtagtcca
    13621 ttcggatgtt aaatccttgc agtttggcga tgttttccat caattttaca tcgatgcctt
    13681 cgtactcgcc ctttgcgttt tgaaattcaa atggagcaaa agcagagtca ctggcaatta
    13741 cgtatacgtc ttttttgggc tctatttttt gcattgtctc gcttgtgctt gactctgccg
    13801 ttgtagtgtt gtcagcttgt gcaacatgtc caaacgcaag tgtcacaagt cctgtcatta
    13861 agaaaacaaa tgagtgaaat aaatgctttc ttttcataat caacctccta aatttccaat
    13921 attttattat tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga
    13981 aaaaaatgcg agtttctaac attttatgtt ataaaacgta acatgcataa taagataaaa
    14041 aagtggcgaa agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc
    14101 ttttcttttt attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat
    14161 tgagagagat acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca
    14221 gcctgaagcg attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt
    14281 attaggtgcg actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa
    14341 taaaccaaca ctaattattg cccataacaa aacattggct ggtcagctgt acggggaatt
    14401 caaggaattt ttccctaaca atgctgttga atattttgtt tcttattatg attattatca
    14461 gcctgaagct tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga
    14521 tgagatcgac aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat
    14581 cgtcattgca tccgtttcat gtatttttgg attaggttcg ccttttgagt accaaaaaca
    14641 agtagtttcg attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt
    14701 cagtatccaa ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg
    14761 tgatgtcgtg gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt
    14821 tggagatgag gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga
    14881 aacggaacac gtggcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga
    14941 gcatgccgta gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga
    15001 aaacaaactt ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat
    15061 gttagaaatg ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa
    15121 agaaggtgaa ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc
    15181 ggacgaatcg cacgtaacca tgcctcaagt cagaggaatg tataatggtg accgagcgag
    15241 aaaacagatg ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact
    15301 tcggttagaa gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg
    15361 accatatgaa catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct
    15421 gcttgaccca gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga
    15481 aataaatgaa cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat
    15541 ggcagaagac ctcacagatt atttcaaaga attagggatc aaagtcaaat atttgcatag
    15601 tgatatcaaa acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga
    15661 tgtgttagtt ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt
    15721 tgctatcttg gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac
    15781 gattggacga gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac
    15841 agattctatg cgtctagcga tggatgaaac atcaagacgc cgtacgattc agcagaagta
    15901 caatgaagaa catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc
    15961 tattacgaaa gaatcggaag atgatacaaa agaagcggta caagtctcgt atgaagaaat
    16021 gacgaaagaa gaaaaagata cgttattgat gaaattagaa aaagaaatga aaaatgcggc
    16081 gaaagcattg gattttgaaa cagccgctaa tgtgcgagat atgattttag aattaaaagc
    16141 ttcaaagtag gaggacaaca tggctaacga taaaattacg attcacggtg cacgtgccca
    16201 taacttgaaa aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt
    16261 gtccggttct ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag
    16321 atacgtagag agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga
    16381 cgtagatagc atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa
    16441 aaatcctcgg tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt
    16501 tgcacgtgta ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt
    16561 agatcaaatg gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc
    16621 accgatcgtg gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga
    16681 gggatacgtc cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct
    16741 ggagaaaaac aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg
    16801 gattcgttct cgtctatttg attctttaga ggcggctctg cgattggcag acggctatgc
    16861 tctggttgac gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta
    16921 ttgcggattt actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg
    16981 agcttgtcca gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat
    17041 tccagataga acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc
    17101 tcagtattat cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac
    17161 accattcgaa gaattaccgg aagagcatca ggatatcatc ttgaatggga cagatgatct
    17221 tttccatttc cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga
    17281 aggggtattg aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga
    17341 gcaaatgcgt ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa
    17401 tccccaagct ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc
    17461 tattcaaaaa gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat
    17521 tgccaaaccg attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact
    17581 agactatcta actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat
    17641 cagattggct acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc
    17701 atcgatcgga ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg
    17761 tgatcttggc aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga
    17821 ttatctgatt gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg
    17881 cactcctgaa gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa
    17941 aaaatcaatc cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac
    18001 tggtgcttca gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt
    18061 agcagtcaca ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa
    18121 atcgttagcg caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc
    18181 cggctacgaa agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc
    18241 gagaagtaat ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca
    18301 aacgaacgag gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg
    18361 cggtcgttgt gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc
    18421 tgatgtctat gttccttgtg aagtatgtca tggcaaacga tataactctg aaacattaga
    18481 agtgcattac aaaggaaaaa gcattgctga tattttggaa atgacagtag aagatgctgt
    18541 agaattcttc aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg
    18601 cttaggttat gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg
    18661 gatgaaactt gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga
    18721 tgaaccaacg acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag
    18781 attagtagat gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac
    18841 agcagattat atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc
    18901 gactggaaca ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa
    18961 aaaaataatg gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga
    19021 atttttgctt gttttattaa tatcagtgtt ctagaatcat tacatggtag ttctttctac
    19081 actgattggt atagacaaaa acttcctata agttcaatcc gttcaaaaaa gaattgagca
    19141 atcggaggag tttcctttga ttgctttttt ctttatggta tgatattcct tgtgtagttg
    19201 gaaaagaaaa acaagagatt atgagtaagt gagagataat gatgaacagt attgaaaatg
    19261 aagaatttat ccctatatta ttaggaagcg acatgaacgt ttatggtatg gcacgttctt
    19321 ttaatgaagc atacggcaaa atctgccaag catatgcttc ggatcagtta gcccctaccc
    19381 gctacagtaa aattgtcaat gtagaagtta ttccaggatt tgataaagac cctgtgttta
    19441 tcgagacgat gcttcgtttg gcaaaagaaa gatattctga taagagtaaa aaatatcttt
    19501 tgattgcttg tggtgatgga tatgcagaat tgatctccca acataaacaa gagctgtcag
    19561 aatattttat ctgtccatat attgattatt ctttatttga aagactgatc aataaagtaa
    19621 gcttttatga agtttgtgaa gaatatgatc ttccgtatcc taaaacattg atcgtcagag
    19681 aagaaatgtt agtaaatgga catttggaac aggaattgcc ttttgaattc ccagttgctt
    19741 tgaaacctgc aaacagtgtg gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt
    19801 ttattttaga gactagagaa gagttcgatt taattttagg ccggatatac gaagccggtt
    19861 ataaaagtga aatgattgta caagatttta tcccgggtga tgatagtaat atgcgtgtgc
    19921 taaatgcgta tgtagatgaa gatcatcaag ttcggatgat gtgtcttggt catccactgt
    19981 tagaagatcc tacaccagca tcgattggta attacgttgt gatcatgcca gattataacg
    20041 aaaaaatcta tcaaaccatt aaggcatttt tggaaaaaat cgaatataca ggctttgcca
    20101 atttcgatat gaagtatgat cctagagatg gagaatataa attatttgaa atcaatttga
    20161 gacaaggtcg tagcagtttc tttgtgacat tgaacggctt gaatctggct cgtttcgtca
    20221 cagaagatcg agtgtttaac aaaccctttg ttgaaacgac ctatggaacg aatcaatcag
    20281 ataaagcaag attatggatg ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa
    20341 atgaagacaa aaaactagct gagcaaatga tcaaagaaaa tcgttacggt actactgtgt
    20401 tttatgaaaa agaccggtca atcaagcgct ggttgttgat gaaatatatg ttccataact
    20461 atattccaag attcaaaaag tattttcatg tgaaagaagg ctagttcatg gagaattttt
    20521 tcagtatttt aggcggaatg ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc
    20581 accgaacgaa agctacaaaa gatcaagaat atttgaatta tgtcttattc aatcatgcaa
    20641 cagttcctga tcgtacagct tatattttag atcgatcaga agaaaatcca atgccgtttc
    20701 tgctggatga tattgagaaa caaaatttat tgcagccgaa cttcattgtg ttgacttgta
    20761 acaccgcaca ctactttttt gaagaattac aagcggcaac ggatattcct atccttcata
    20821 tgccaagaga agcagcaaat gaacttgtcc gtcagcatac gacagggaga gtagcgattc
    20881 taggaacaga aggaagtatg aaagctggaa tctatgaacg cgaagtcaaa aatcttggat
    20941 tcgaaacagt gattcctgat actgttcttc aagaaaaaat caattatctg atctatcatg
    21001 agatcaagga atcggattat ttgaatcaag agctatatta cgaaatctta gaagaagcgg
    21061 tagagcgcct aaactgtgaa aaagttatct taggttgcac agaattgtca ttgatgcatg
    21121 aatttgctga agataaccat tatccagtga tcgatgccca atctatttta gcagatcgga
    21181 cgattgaacg agcgctagct gaaagaagca aggcattgga tacagcatcg gaaaagtaaa
    21241 aaaattggat ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga
    21301 aaaagaaaac tggtaatgat acgcccggaa tgatataatt ttactatgca caatgtgaga
    21361 ggagttcgta attatgacag ataatttgca attagtcatc ataacaggta tgagcggtgc
    21421 agggaaaaca gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat
    21481 gccgcctcga ttgattccaa aattctggga attaatcaaa gaatcaggca aagtcacaaa
    21541 aatggcttta ggagtggatt tacgttctcg ttccttcttt gaagaaatcc aaaacatgct
    21601 gatcgaaatc gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga
    21661 cgaagaacta gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg
    21721 tttgatcact gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc
    21781 ttccgtgatc attgatacga cgacattgac accacgacaa ttacgtgaaa aaatcaatga
    21841 gtcattcaaa tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa
    21901 atacgggttg ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca
    21961 ttatatcccg gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat
    22021 gtcctttgat gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt
    22081 gcctggatac attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg
    22141 tcaacaccgt tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa
    22201 agtcaatgtg acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa
    22261 atgaaaacct accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg
    22321 ccagttatcc tgaaaagttt gcgaaatcaa ggagcagata tcacagcagt agtgactgtt
    22381 gcagatgatg ggggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt
    22441 gacttgagaa atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc
    22501 caataccgtt tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc
    22561 attgccgcag tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa
    22621 atgatgcatg tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct
    22681 gtctttaaag acggaacgac tgcagtagga gaatctaaaa tcgcgatcga ccgtaaaacc
    22741 attgaccgtg tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa
    22801 gtcgtttcag caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc
    22861 agtatccttc caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct
    22921 gaaaccgtct atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat
    22981 gccgatcata tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg
    23041 gtcaatacgg aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta
    23101 gtacaagtcg cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc
    23161 gactttttgg aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa
    23221 ttgttccgtt tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag
    23281 aataggatgt catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga
    23341 gaacatgcga aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg
    23401 aaccagcagt ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat
    23461 tctcttttga aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa
    23521 ttaaaaaaga acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg
    23581 gatcttgata tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga
    23641 aattcgcaaa aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat
    23701 aatccggaaa cgagccgtta tcatttggaa atattttcta tttatgaaca gcataaccaa
    23761 gacatttgcg atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat
    23821 ggctatattt cttatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt
    23881 gcaaccaact caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg
    23941 gtcaatcgtc tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct
    24001 aaacaaatcg aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa
    24061 aaacttcagg aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta
    24121 ggggaaatga ttccctcagg tgcgatatca aaatcgggaa ttaatcaccg aattcgcaaa
    24181 ataaatgaat ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat
    24241 acatgaagca ggagatgcga atgtatcagt tttttttaaa agttatactt gcttttcttc
    24301 cgtcaaacta taattccatc tttactggag taacataatt tggaaaggct tctattgttt
    24361 ttaatacaat cgtataaaaa gcggggagac aactgaatga agattttagt tttgtacata
    24421 aatttttgct taatatagaa gtcctgatat agataagatc aagtatttat ctataactgg
    24481 cctgattaat taaaaaactg gaaggaactg ctcgatcatc cttgagaact gctcttctta
    24541 cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc attggattag
    24601 ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt
    24661 tattcagtga taatcataag ttcataaata gtaattgaga tgtatggttg gaatacgtta
    24721 tacctgaact tgaactacca agatttggtt tagaatttga gaatcgatgt ttataaaggg
    24781 agggcaaaaa tgcgtttttt tacaactttt actatcatta tgattgcagt actttttatt
    24841 tttctagata tagcaaagcg aaatacagca tttttattat atcgggtttt acttcgagca
    24901 ggtctcataa catttatatc gatcgttgga ttttttctat tcacagttat tgtatttatt
    24961 tggagaactc cagcaccacc attgccagag attacatatg gagaatttcc ttttagactt
    25021 gagtatgaat tgaatgaaga attacatgtg atagaagaca cgttaatagt tgaatttgat
    25081 ggatttggaa tgaacgaagg catagggaga tatcgtagat ggacaagtcg tttagccagc
    25141 ggagaagatt tagtgttgct gttagaagtt agtgataata agcaaatatt ttattttcca
    25201 ggacctgcta attattacat gggagatagg ttgaatggtt ataatcatac ttttcctagt
    25261 gcttctttca ttgaaaggga aagaggcatt actagaaggg atattttaca tgacaaagaa
    25321 ttattagaac aatttggtcc gcttgatcaa aatacaataa atgaagaaga attgttaaat
    25381 caatataata taaggctagt gaattgggaa atatctgaac cgattgttaa caattttggt
    25441 gattagaaaa caattacgat aaatagaaac aagccaaatc atcagcaagt aaaagctgat
    25501 gatttggcct ttcttatacg agtagtagga attcatagcg aatattatta cgatcagtag
    25561 atattttgat attaaaggag tattacgaac tagtatgtga gaatgtagag gtgatttgcg
    25621 aataatgaga aatttatcaa atagaaacaa aatattaatc attattgttg ttatagctgt
    25681 gtttcatctg ggaacgaatg cggttttaag tagaataata ttgggtccta agccaccaag
    25741 acctgcgatt acccgagggg agtttgattt tagattagaa tatgaagtag atggggaaag
    25801 gattgtgata gaagatacaa ttgttgcatt atttgatgga ttttctgcag atgcaggtag
    25861 catggcatgg tatcgcacgt ggcgtttgca tttggcgagt gacaagaggg cacaacaatt
    25921 attgttacat gagttagatg atggtcgaaa aatatattat cattttggag gagctggatt
    25981 ttatatgggg aagttagtag cagtgtaact gtaacaagga aagatttgcc aggtcttggc
    26041 attcgaaact ccatatttta tatcgagcgt gggagagata catctattgg gaacaggaaa
    26101 gggttaactc ttgaagagtt atataatgac tttggaatcc gtcttgttag ttgggaacac
    26161 aatccaccaa tagaaaatcg ctttgaataa tcgaaatcta ttaatccatt tcatcagcca
    26221 gtgaatgttg taatggaaca atttggttta gtgttcaaaa ccattgaaat actaggctgt
    26281 gatcagcaaa aatgtatctg aataaaaaag agagcaacta ttaaatgaga ggagttagga
    26341 gttgttagct ttatacgaat caaagtggca aatacagatg cgtatgcatt tgtgtttgcc
    26401 atcttgcgtt tggtagacga gataggtata agctaaagaa acaacttgtt taaaataaac
    26461 gacctagctc aacaaacgac catctaatca actaacagca tacataaaag caactatttt
    26521 tgaagtagaa ttgaaatcgg gataacttaa aaagtgattg ggatactagt gtgaatcacc
    26581 gtacataaat attactcgtt aagggaattt gagaaatttt gtatgaaaat ttaagaatta
    26641 gttagtatgc gcaagtcaaa aatatgaata aattatttgg tggagataat ttgctcagct
    26701 tgtcataaga aatgagataa tctggcagat gcaagctttt ggacaccatt tattcggaat
    26761 aaggaggctg ggacagaagc gtttaactcc aagaaataag aaggagttgc ttctgcttcc
    26821 gccgtttata ggtgtttaga gcgtgagaca aaagtgttct ttacttttgt cctacgctcc
    26881 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
    26941 aagatttctt tacccaagat gacagtcgga acaaatcgaa tatgtgcttg ctcagcttcg
    27001 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
    27061 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
    27121 tgtttgatct ctttgatcgc tttttgacca tcgctgattg tgagataccg gtgcatcacg
    27181 tttcctcgct gcaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
    27241 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
    27301 ggacaagata ggttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
    27361 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
    27421 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
    27481 ctaaaaatta gatgaccaat tcaaatagac atatgtgaaa tatttttata aataaaagaa
    27541 aaaatggaat gttttacgtt ataatggaac agataataag agagaatatg aacaaggatg
    27601 atgaatcatg cgaaaaatga aaacaatgga tggcaatacg gcagccgcat atatttctta
    27661 tgcatttacc gagattgctg caatatatcc aataacaccc agttcgacca tggctgaatt
    27721 agtagatcaa tggtcagcag caggaaaaga aaatgtattc ggtcaaccag taaaagtagt
    27781 agaaatgcaa tccgaagcag gagcagctgg tgtggtccat ggatcattga aaactggtgc
    27841 actgacaact acatataccg cttctcaagg gctgcttttg atgataccga atatgtacaa
    27901 gattgctggg gaactactgc caagtgtttt ccatgttgct agtcgggcag tgacgacaaa
    27961 tgcgttgaat attttcggag atcacggaga cgtgatggct gcaagacaaa caggatttgc
    28021 tatgttagct gaaagcagtg tacaagaagt gatggattta gcacctgttg cccatcttgc
    28081 ttctattgaa ggagaagtac cttttttgaa tttctttgat ggattccgta cgagtcacga
    28141 gattcaaaaa atcgaagttt tggaatatga agaattagct cctctgatca atcaggagaa
    28201 attagcttca tttcgtaaac gagggatgaa tcccaatcat ccgactgtta gtggtacgaa
    28261 ccagaatcca gacatccatt ttcaacaacg ggaaacgatc aacccctatt atgataaatt
    28321 acctgaaatc gttcaaaaat atatgaaaga gatcaaccga ttacgaggaa cttcttatga
    28381 cttggtgact tattacggag cagacgatgc ggaagaagtc attgtttcta tgggatcagt
    28441 tgcacagacg attgaacaaa cgattgatta cttgaatgca aacggacgga aaacaggttt
    28501 tttgaatatc catctttatc gtccttttcc agtggaaaca ttcgtgcaaa agttgcctca
    28561 tacagtaaaa acaattgctg ttcttgatcg aagtaaagaa ccaggggcag gtggagaacc
    28621 tctcttatta gacgttcaaa gtgcgttata tgattctgaa cttcgcccgg ctgttattgg
    28681 tggtcgctac ggattggggt ctaaagatgt gactcccgac caaatctcag cagtttttga
    28741 tgaattgaaa aaagacccaa gcatacgcaa aaaacggttt accataggga tcgtggatga
    28801 tgtgacttat caatctttgg aaccaaagga aagtctggat ttgacagaac cgcaaacatt
    28861 ccaggccaag ttttggggat ttggatctga cggaacagtt ggagcaaata aatcagctat
    28921 caagattatt ggagatcaca ccgataaata tgcgcaaggc tatttttatt atgattcgaa
    28981 aaaatctggc ggtctgactg tctctcatct tcgttttgga gatacgccga tacgttcagc
    29041 gtatcttgtc gagcatgcgg accttgttgc ctgtcacaca ccagcttacc tgcatagcta
    29101 tgacttggtc aaaggattga agccaggtgg cattttttta ttgaatactc tttggagcga
    29161 cgaacaatta gaaacgcatc tgcccttaaa attaaaacgt tacttggctg aaaataacat
    29221 tagattctat acgatcaatg ccatgagatt ggcccaagaa gtcggtttgg gacgccggat
    29281 caatacggca atggaaacgg ctttttttaa attagctgac atcattcctt ttgatgaggt
    29341 gcttccgctg ctaaaagaag aagccctgaa aagctatgga cataaatcaa tggctatcgt
    29401 agaaaaaaac atacaagcaa tcgatcggac gaccgaactg cttcatcaag tcgcgatccc
    29461 agaccattgg agaacgttgg agatgcctga aaaaatcaat aaaaggaaca ctggttatat
    29521 acaagaaata cttgaaccag tgaatgcaca ggaaggaaat caactctctg ttggtacact
    29581 tgtagcaaat ggaatgacaa ctggcgaaat gcctttagga acggcttcta tggaaaagcg
    29641 gggcgtcgct ttagaagtac ctgagtggat tagtgatcgc tgtacgatgt gtaatgaatg
    29701 tgcctttgtt tgtcctcatg ctgccatccg tccattttta gcagatgaag aggaaatgga
    29761 agaagcgcca gaaggattta tcgtgagaga aatgcgaggc gcagacggcg taaaataccg
    29821 tatccaagtt tcagtggaag actgtacagg ttgtggtctg tgtgtagagg cttgtcccgc
    29881 aaaagaaaaa gctttagtga tgaagcctta tgaagaacag aaagaacaag ctgtgaattg
    29941 ggcttttgca atgacattaa gacaaaaagc aaatccagtc aaaggaaaga atactgtctt
    30001 gtctacacaa ttcaatcaac cgcttcttga attctcgggc gcttgctcag gctgtggcga
    30061 aacaccttat gtaaaattat tgacacaaat gttcggtgat cgaatgatga tcgcgaatgc
    30121 gacaggctgt tcttctattt ggggcggtgc ttctcctgct tctccttata cgaccaatga
    30181 atgtggtcaa gggcctgcat ggtcaaattc tttattggaa gataatgctg aatttggtta
    30241 tggtatgctt ctagcggcac agactcgtcg cgaggctttg gcaagttcaa tcaaagaagt
    30301 attgccaaaa gtatcagaag aactacgaga attgatggaa gactggttgg tacacatgtt
    30361 tgagagcgaa ggaacgcagc aaagagcagc aaaactgcaa gctgcgatcg aaagcgaacg
    30421 atcaattgtc cctgaactgg aggatatata caagagaaag gatctattcg taaaaaacag
    30481 ccaatggatg attggtggag acggatgggc ttacgacatt ggttttggcg ggatcgatca
    30541 tgtacttgcc agcggagcag acgtgaatat tcttgttttg gataacgaag tctattccaa
    30601 tactggcggt caaacctcaa aagcaacacc agcttcggca atcgctaaat tttcggctag
    30661 tggaaagtat gcttcaaaga aagatctagg aatgatggcg atgacatatg gaaatgttta
    30721 tgtggcgcaa attgcttcag gtgccaatca aatgcagacg ataaaggctt ttgaagaagc
    30781 agaaaagtat cctggacctt ctttaatcat tgcctatacg ccttgtatca cacatggttt
    30841 gcttggtgga atgaaagaat cgcttaaaga agcaaaagaa gctgtttctt ctggctattg
    30901 gtcattatat cgatataacc ctgcaatggt cgaaaaaaac aagaacccaa tgactttgga
    30961 ctacaaaaaa cctgatttct ctgctatgcc tgatttcatg aggaaacaag ttcgcttttc
    31021 ctcgctagag aacgcaaatc caaatcttgc aaaagcttta tttgaaaaga ctatccaaga
    31081 cgctaaaaca cgtttttaca actatgctgc actgacggga gaattagaaa aaatcaagaa
    31141 acgacttgaa ccagaggaat ctatcgatgt acccgttaag actagaaaag aaaaaacgac
    31201 ggaccttgaa gcagaagaaa gaagagctgc cagagcagaa cgacgtaaaa aaatggaaca
    31261 agctacaaaa gaaaaaaagg aaaaataaca gaaaaaatat aatgaataat t
//
LOCUS       DAZKSU010000028        31231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000028 DAZKSU010000000
VERSION     DAZKSU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.028"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(400..717)
                     /locus_tag="ACG8Z3_001320"
     CDS             complement(400..717)
                     /locus_tag="ACG8Z3_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0823644.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYEMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(718..972)
                     /locus_tag="ACG8Z3_001321"
     CDS             complement(718..972)
                     /locus_tag="ACG8Z3_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0823645.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1232..1372)
                     /locus_tag="ACG8Z3_001322"
     CDS             complement(1232..1372)
                     /locus_tag="ACG8Z3_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /protein_id="HFQ0823646.1"
                     /translation="MYRVASEIQENKVIPVLLYGTHNNFYELAENTRNFIRVDKSASV
                     EL"
     gene            complement(2011..2097)
                     /locus_tag="ACG8Z3_001323"
                     /pseudo
     CDS             complement(2011..2097)
                     /locus_tag="ACG8Z3_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            3263..4303
                     /locus_tag="ACG8Z3_001324"
     CDS             3263..4303
                     /locus_tag="ACG8Z3_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0823647.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SSPSTITKDWLE"
     gene            5144..5476
                     /gene="traE"
                     /locus_tag="ACG8Z3_001325"
     CDS             5144..5476
                     /gene="traE"
                     /locus_tag="ACG8Z3_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0823648.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            6095..6436
                     /locus_tag="ACG8Z3_001326"
     CDS             6095..6436
                     /locus_tag="ACG8Z3_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0823649.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            6455..6745
                     /locus_tag="ACG8Z3_001327"
     CDS             6455..6745
                     /locus_tag="ACG8Z3_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823650.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
     gene            6937..7170
                     /locus_tag="ACG8Z3_001328"
     CDS             6937..7170
                     /locus_tag="ACG8Z3_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823651.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARGYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWREHNGIKDLKPARPVIRRKKLKK"
     gene            7237..7490
                     /locus_tag="ACG8Z3_001329"
                     /pseudo
     CDS             7237..7490
                     /locus_tag="ACG8Z3_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7576..8247
                     /locus_tag="ACG8Z3_001330"
     CDS             7576..8247
                     /locus_tag="ACG8Z3_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0823652.1"
                     /translation="MKKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPID
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLTLPIFK
                     GANQYTLSLGAATYFYEDAEMRKGNYVLAGHNMEIPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            8300..10279
                     /locus_tag="ACG8Z3_001331"
     CDS             8300..10279
                     /locus_tag="ACG8Z3_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0823653.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDVDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQAL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNNS
                     KGNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELT
                     KFGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVE
                     VPSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVID
                     KTVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDT
                     AGQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASL
                     HGQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLK
                     VDSGLFGTGIKATPLESAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKS
                     DKEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKAS
                     VPMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            10294..11046
                     /locus_tag="ACG8Z3_001332"
     CDS             10294..11046
                     /locus_tag="ACG8Z3_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0823654.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            11062..11823
                     /locus_tag="ACG8Z3_001333"
     CDS             11062..11823
                     /locus_tag="ACG8Z3_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0823655.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            11836..12096
                     /locus_tag="ACG8Z3_001334"
     CDS             11836..12096
                     /locus_tag="ACG8Z3_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823656.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            12093..14183
                     /locus_tag="ACG8Z3_001335"
     CDS             12093..14183
                     /locus_tag="ACG8Z3_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0823657.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            14210..14674
                     /locus_tag="ACG8Z3_001336"
     CDS             14210..14674
                     /locus_tag="ACG8Z3_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823658.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            14671..14913
                     /locus_tag="ACG8Z3_001337"
     CDS             14671..14913
                     /locus_tag="ACG8Z3_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0823659.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            14931..15233
                     /locus_tag="ACG8Z3_001338"
     CDS             14931..15233
                     /locus_tag="ACG8Z3_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823660.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            15303..17366
                     /locus_tag="ACG8Z3_001339"
     CDS             15303..17366
                     /locus_tag="ACG8Z3_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823661.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHEGRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYSTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEKENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHEIAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIRERKEEFYQGLRTDKLKISKELNEQLNKLYQIT
                     SEALMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEE
                     AQEQARKEYEQQLEELLNRTNQATTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            17366..19975
                     /locus_tag="ACG8Z3_001340"
     CDS             17366..19975
                     /locus_tag="ACG8Z3_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0823662.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAILMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPENDYRQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHQGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     TNSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLHQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            19972..22368
                     /locus_tag="ACG8Z3_001341"
     CDS             19972..22368
                     /locus_tag="ACG8Z3_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0823663.1"
                     /translation="MIIGGIFSGIANWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLETDPNKVESLPTDKRNALTAKNFKTANINSVII
                     PKEAAKMADEDKSGLSDIQKDRLRALQYRLELDDNGKEIPVEISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSNLNDIRKNGF
                     GNRKDVIPDNGEEQPDDKATIVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQY
                     RYKSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSMKIGLPKTQNLP
                     MKDSRLNQFKENDQSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNVSIQN
                     VVTREAAPEPIQKVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLRDIKQDIKP
                     "
     gene            22420..22752
                     /locus_tag="ACG8Z3_001342"
     CDS             22420..22752
                     /locus_tag="ACG8Z3_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823664.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            22753..23346
                     /locus_tag="ACG8Z3_001343"
     CDS             22753..23346
                     /locus_tag="ACG8Z3_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823665.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVQTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            23509..25203
                     /locus_tag="ACG8Z3_001344"
     CDS             23509..25203
                     /locus_tag="ACG8Z3_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0823666.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASRLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            25891..27768
                     /gene="ltrA"
                     /locus_tag="ACG8Z3_001345"
     CDS             25891..27768
                     /gene="ltrA"
                     /locus_tag="ACG8Z3_001345"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0823667.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            27899..28276
                     /locus_tag="ACG8Z3_001346"
                     /pseudo
     CDS             27899..28276
                     /locus_tag="ACG8Z3_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            28276..29481
                     /locus_tag="ACG8Z3_001347"
     CDS             28276..29481
                     /locus_tag="ACG8Z3_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0823668.1"
                     /translation="MENQDESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNDNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            29497..30141
                     /locus_tag="ACG8Z3_001348"
     CDS             29497..30141
                     /locus_tag="ACG8Z3_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823669.1"
                     /translation="MENKTLKIVLTAALSISLIGNIALFWQLKEEQATNTYKIEEVAK
                     NASEKLKKNFSEAQAKIDKLNQTINEKNQEIDQLKQQDGIKAKEEVTDAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAETS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            30152..30958
                     /locus_tag="ACG8Z3_001349"
     CDS             30152..30958
                     /locus_tag="ACG8Z3_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0823670.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFTILGFLILASPIYTHKKVNI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQGLLKELANLDYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIVPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDDNYEQHAINYKVKGYKKEQKAAQKQIESLQATIDLNEELVNKLKDQLP
                     YQVADDQENTENKINDYQQEIETSKTQMKDQEEIIQKIQEKIDRMTKENI"
     gene            30981..31211
                     /locus_tag="ACG8Z3_001350"
     CDS             30981..31211
                     /locus_tag="ACG8Z3_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823671.1"
                     /translation="MKGANLVGKDIFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKIRSFEALYLAAKKYFTAERK"
BASE COUNT        11448 a         4816 c         6203 g         8764 t
ORIGIN      
        1 catagatata ttggacttcc tcttttaaaa cacaaaaata aatactacct tatttaggta
       61 gtatttatta aaagttacgt caattttgat taaaaatata ctacttgaac ctctcatgag
      121 taaagtcacg aaatttttgg gaatagagcg tacttgatat cttatttttt cactaatagc
      181 agtgtctctt attcaccaag cgatccccgt agttcctacg gttcttaatt tttctctaga
      241 ctaatgattg tatctttaga ccttcggcca taacatggag caaaataaaa aggtactaag
      301 tcttatatat caatcttcga gtaaccgatg gttaacaacc atatcgagcg gcattaatca
      361 cccacctgta gaggatgagc gacttttgcc taattattgt taaaagccta gtttcatttg
      421 tttagattgg aaaatacgtc ctacaagtat ctcttttttt tcatggtcga tccgataaaa
      481 taaggcatag cttttgccga tcacgatccg atatgttggg atatccattt catagatttc
      541 ggaaacttcc tcatgcatag ttggaaaatt tttggttagt tctaaagcct tctggatcgc
      601 acctacaaat ctttttatgc tggctggaga aagagcaagc tcattttccc attcttctat
      661 tatcgtttca aggctggtct ggaaactttt agaataaact agcgtgtact ctttcatcta
      721 ttcactccag ccgtattttc cgaaagcttt taccatttca atttctgaaa ctgttaaacc
      781 tttctcaaag tcttggaaac tttcttcaat caatttgcga tcgtgttctt ttgctatttg
      841 agtagtatct aatatgaata agttgccttc ttgtttggct tggatagtgt ctccgctttt
      901 taatccaaga gagcttggaa ggatcactcc cacgctattg ccgatctttc taacctttag
      961 ttcttttccc atgttatcac actcctttta gtttttgata aataaattat aacatgttat
     1021 aacaacaatt gccaacggtg tttcttaagg gattccactc catattatta ttggacaaaa
     1081 taagccactt agaacgctga aaacatatac aaacgatatc aaaatacgag tgaactactc
     1141 accatacact tccgttaaca tccatttaag taggtatttt tattatatat agatttaaca
     1201 tatgtcggtc ggttgattga tatagctaaa gttataactc aaccgaagcc gatttatcta
     1261 cacgaataaa gttgcgagtg ttctcggcta attcataaaa attgttatgt gttccatata
     1321 gcaatactgg aataacttta ttttcttgaa tctcagaagc aactctatac acggtgaccg
     1381 catacttaaa tccattagac caattacctg ataaacctcc agttttttgc tcttacttaa
     1441 atggatcaat agaaatttca tcacaaacct atagaggatg agtgacttct gcctaattac
     1501 gttaaaaaat attgaaagta actctatgta gcaaaaaata tctagaattt catttcctca
     1561 aaactgatcg atattgtatc caaaaatata tactacctaa aataggtagt atatattaaa
     1621 atatgtgctt ttgttagaaa aatcaataca taaattatca tatctgtata tgtcatttag
     1681 gtattttagt tatgttataa tagttttgct gtctgctacc ccggcctaat ctggcaaggg
     1741 gaggaggaga tataatgaaa tacctttttg attttgtttt tgcaatcaag ccatttgcag
     1801 aggtaactgc acactacatc tgcaagtggc ttgattgcaa aattgccaaa aaagacagta
     1861 agcctacccg ttaattctat aatatagaaa cgtaaaaaaa aaccccatca gcctgcacgc
     1921 tgatggggtt tggtgatata atgaaatacc tctttgttac tcataattat accataatta
     1981 gtcacgaatg caaccaatag aaaaaactac ttagaacggt agatcatcgc taataacaat
     2041 gcattgtcca ccaaatggat tttgtgaagc attaaataca ttgttattaa ttgtcgctgc
     2101 ttttaactag gcctctcccc ttctattata aaagaagata tatatcattt cttaattacc
     2161 tactacctta aaccaaaatt taagtactac ctaatttaag tagtacttaa ataatctaaa
     2221 aaatagttat cccaattgtt atgattttat aatacgatta tttgtaacaa aatgaatatt
     2281 atataattaa acactaccat atttaggtag tgtttaatta ttagcaaaac aaaaaatatt
     2341 tattctattc tcttaacttc aactaagtta tgctgcacat ctttatataa atcatatgca
     2401 actcattagc tagacaaact acaagcatgt agttttatct gttaattatg tactacctaa
     2461 ataaagtagt ttataattaa caagtatata tatttaaaaa tttaaaatta atttttttat
     2521 gacttgtaca aatttatctg atattctctt attaagaact acctaatttg ggtagttctt
     2581 attttttaca ttttaagtag tatttaattg caatttggta gtattttttt attatcttat
     2641 tcctagaacg gtagtactta atataaatat aggtagtatc taatttaagt attaatggta
     2701 gtatctaatt taacaggtag tatgtaacta ctttaaagta atttttttca gattaacaca
     2761 gtagtatctt attaaataag ttaattctaa aggtagtaca taattattat atatatctaa
     2821 cattggtagt acttattgta ttttttagta gtatataatc tattgttttc tagcctaaag
     2881 ccttgtctta taagaataat ataatatata ctactgaaat aaggtagtat atattactag
     2941 tagtggtagt atgttttata tgcataaggt agttcttaat ttattttttg ttttgatact
     3001 ggtagtactt aattatcctt aattttaaaa ccaaatttta ttcatacgtt cttctggtag
     3061 tatttaatta taaataatat ttatttaaag tagttcttaa tgactttaaa ggtagtacat
     3121 aattcatttt atttcttatt ttaggtagta tttaattaaa aaacagtttt aatgcgtttg
     3181 cattttttat ttatctttgc tattttcgaa aagagtaggt aattttacat aaataaaaca
     3241 acgtgaaagt agaggatcta caatgactga ttttaatttt tttaaggcgg atagagttta
     3301 caacgaatta ttttatcaat ttccaaaagt ctttattgtt tctgacgaat acaaaaaaat
     3361 gaaagattca actaagattg cctatatgct tttaaaagca agattagaga tcgcaatcag
     3421 caaacggcaa atcgatgaag aaggtaatgt ttattttact tatacgacaa atgaactatg
     3481 tagcgtatta aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt tggaatcctt
     3541 tggtttatta ttacaaaagc agatgggatt taacaaacag ttagggaaaa ataatcctaa
     3601 tagactatat ctagcagaat taaaagtctc agaaaatgat atctacttac tcgaaaaatt
     3661 tgatagagag aatagggaaa atgttgataa atcagagggt atgaaaatca tacccaccct
     3721 cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa tcataccgtg
     3781 ccaaaacgtt gataaatcag agggtatgaa aatcatacca gaacttaata ataatatatt
     3841 agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc aattgttgtt
     3901 agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc ctgaacgagt
     3961 attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa ccgtccagac
     4021 tattcataat gcaaagaaaa aagctgaaat agaaagtggt atttcgatag tttttgaaga
     4081 actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt taaaagccta
     4141 tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg tctatgtaaa
     4201 aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac gtttgaattc
     4261 tgaaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta ctgacaaagt
     4321 gattgttgct tcatggagta acattaaagt taaaaatttt ttattattct aattatatta
     4381 cgaatattac aaaataatag ctattttaat agaatttata agttaaaata ttatgataaa
     4441 aatcacaaac gtagcctttt acgtcataag tgttaaaaga aataattaat ttcaaatata
     4501 aaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata cgcgaagaga
     4561 gataatttca ttatttaaac aaactaaaaa tcttgatatt acttcttttc aggcaatacg
     4621 aatattattc gcttacattt ttttgtataa atttagttgt tttaaattta attgtctgct
     4681 atgcctgtga tgaattgatt ttcgttctgc acttttggac gataaatttg gacaattgaa
     4741 aatacaaaaa aaaagaaagg cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc
     4801 atacacggca ttaagatttg gcccttaatg cgttaacctt tcagtgttac ggtattaccc
     4861 gcaatggatt tagtataaca gatattcaaa agtgatacca ctttttttgg ctgtgttaag
     4921 gttaaaaata cgtgatatta cgatgaatct atgcctaagc ataatgttat aaaaatgtcc
     4981 gtgtggctct ttgccgtatg acgaacgctg aaattagagg gaacgcatgt ttggatgcgt
     5041 tccctctttt ttgtttgtca tgaacgaaaa tcaattctct atttttgaaa aataacaaga
     5101 aaaacttgtt aaaaatattt aaagaaattg ggtggtttat tttatgtcat atacaggaat
     5161 tttgagtctc aaagatattt gtcactacgg taaacggtgt accgctacag agaaaatcac
     5221 gaagaaacta tcaactggcc aaagtaaaac cgttgtgcag tgtaagaaat atataatcca
     5281 gaaagacaaa gtctcagagg aaatgattta ttacattggg aaacaaaagc aaataatttt
     5341 aaaagatcct atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa
     5401 cggtgtactg attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg
     5461 gcgtttaatc agctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct
     5521 tgaaaatatt gtcgagactt ctaaaagaac ttagaatcat tagaaaaata aggagtagac
     5581 ttgatctatc aacaatttcc ttcggggagt gacggggtag atttttacga ttggtaacgt
     5641 aaaaaattgg tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta
     5701 aataatatac acagagtaaa caagagggaa tttagaggtt agacaagtta gaatagcgcc
     5761 tagattaatc atgagcagct actaagggat ctaggctttg acgcaaactc atggaataag
     5821 gagtaggtct tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca
     5881 tgtagatact tgcgaggacg ttggtcattg ggtcaatcga gcatttaccg ctgtctacta
     5941 gggtttctcg tttcggacgc cataaacgag tcgatatttt ttatgtattt ttaagtaaac
     6001 gaaaaacgat aaaatatgaa cgagtcctga ataaagtgaa aaaattgata taaaacatag
     6061 agaaaatgaa atttacgtaa tggaggaatg acaaatgaaa aattatgatc cgaatatacg
     6121 attgggcaca catactatta aggtttcttt ccaacgttgg gattacaaag gttttgtgac
     6181 gtttagaaga ggcggaaatt gtaaaggatt agatgtttta gcattaaacg aggatgattt
     6241 atacgatcaa acattgactg ataatccaat tggattcggc ttgctacctg aagatgatga
     6301 aggtaatgaa tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga
     6361 agatacttgg tcatatttga gtgattatat tgtcagcgtt gaaatcattg aatttgtagc
     6421 ggataaagaa gaataaaaac tggaggaata aattatgaaa tttttcgaag taaaatcacc
     6481 atactattat gcgttaatcg cagcaaagaa tgaagaagaa tgtgcagaat tgtatgaaca
     6541 agtagtaaca gatatcgaaa atcgtgaaga atttattcga tgtttgcatg agttaaatcg
     6601 aaatgaagca attcaagagg atgcgaaaaa aattagtgaa gaaacgttac aaccaatcgg
     6661 tatggaggaa gctacaaaag acatttttac aattattgat gaacaaaaat cttgtgtatt
     6721 aagcattgat tctacattgg tttaatagat ccaagattct ataatttttt tcaaagtaaa
     6781 aagaaattag gtaatgattc tcagaccaat tacttaggaa aataaccata caaattagat
     6841 tttacagata aaggattaga aggaatcacg gcgattaata aaaaggtagt attaccttag
     6901 gcattgagta aggaatttat ttaggaggaa aattagatgg cgcgaccagt tttagatgcg
     6961 atggatatgg atactttgac aaaaggaagg tatgtacagc tgttagctcg tggttattca
     7021 cctaaagatg tattcaaagc cctatctaaa ggaacagata tggaaaaaga aagattacga
     7081 aaagaattcg actattggag agagcataac ggaataaaag atttaaaacc agcacgtcca
     7141 gttattagac gaaaaaaatt gaagaagtaa gaccaaagcg tatggcgtac ataggctttt
     7201 ttattttgat aaaaataatg aaaggaagga aaactcatga attgtaaaga aatcgaaaat
     7261 agaaaaaaag tgtcgaaaga aatggaagaa aagttactaa aaacaatgaa acaaaaacaa
     7321 tgaaacaaaa acaccttaaa agattatctg tcgcgcaata tatcaatgat atgcagttaa
     7381 caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa ctttgaacag ttgagacgaa
     7441 cttatgtgaa aaaaagttct aattgtcaac tattgttaga agtctcatga cattcattaa
     7501 tagaattcat ttttaaaact ttttctatgg aaatgagaaa aagttttttt gttttgtctg
     7561 aaaggaggca gatggatgaa aaaatggtta tttggttttt taggagtcgc tttaatcgtc
     7621 ttttgcagtg tgtttggcta tgttagctac caaaaacatg aaggagaggt gtttaagcaa
     7681 aatatagaaa aaaagatgcc catagatcaa atcaatgaac atgcaaagag ttataaggag
     7741 gatgcaacga acgttaataa tgatatgtct ttgggacaaa tgttgtccat acaaaaagaa
     7801 gcaatcgaaa tgggcgtcaa taaacaagcc tttgcacaaa ttcagattcc tgcgcttggt
     7861 ttaacattgc cgatttttaa aggtgcaaat caatacacat tgagtcttgg tgcggctact
     7921 tatttttatg aggatgccga aatgcgcaaa ggaaactatg ttctagcagg tcataacatg
     7981 gaaattccag gtgttttatt ttctgatatt caaaagttgt cactagggga ggtgatggac
     8041 ttagttagta acgatggcgt ctatcgttac aaggtaacgc gtaaatttat cgtacctgag
     8101 tactttaaat taatcgatgg cgttcctgaa gagaactctt tcttgtcttt accaaagaaa
     8161 ggagagaaac ccttattaac gttgtttact tgtgtttata cgtcacaagg aaaagaaaga
     8221 tacgtggtac agggagagct acaataacaa ggttttaatc aacgtgagtc gttgaaatat
     8281 gaaacgagga gagtaaaaaa tggcaataaa tcggtatgta aaaaaagctg gaatgcttgt
     8341 tggaatgtta ggaatcattg caggctgctt tcagggatat cgagcatatg cagaagatgt
     8401 cacgcaaaaa acgccaccag agaaggtcaa tatcacggtt cataaattaa tgtatgatca
     8461 aggaacgcaa ttgaatgttg atgttgacgg gatcaaaaac gatggttata cgcacgatca
     8521 atatcctacg ggtgtaacga aatacaacaa ggcagattat ggtgatgttg agtttacgtt
     8581 aggaaatatt acggatcaag cattaccgag ggaagattct gatctaacca acgcgaaagt
     8641 ggacgaaatc gttaaagatg tagaagacaa gggatctaac agtgagtacg tcaagaatgc
     8701 aaccaacaaa attacttcag ctgtagatga aaatggtgag attacttttg ctgatcaacc
     8761 agcctatgtc aacaattcca aagggaatgt atatgtggtt tatgaaagta aatccgctgc
     8821 tggattagtc acacaaaaag caaaaccaat ggttgtgatt gcgccaatga ctgataatac
     8881 aggttcaggt tttttaaaag acattcactt atatcctaaa aatattgttt ctaagttgag
     8941 ttttgaatta acgaaatttg gtgacgatgg cacagctcaa agcaaacaaa cacctttaaa
     9001 aggggcaaaa tttgagttat acaagggcga acctggaaaa ggaacaaaat taggagatct
     9061 tgtatcggat gatcaaggaa agctgactgc tactgatttg acgttaggaa aatactattt
     9121 tgtggaagtt ccttcagaag ttgtagtggg atcagataaa gaaccaacag ccgatcaata
     9181 tttattaggc gcagacgctc gcaacgatgc gcacaataaa ttaacctttg aaattacgaa
     9241 tgatggcgtg acaagtgact taaaagcttc atacgtaaac tataaagcac cagtgatcga
     9301 taaaacagta acaaatggaa caggacaaga acattctttc caaattggtg acgcagtaaa
     9361 ttatcaagga acgattcata tcccaacaga cattgcaggc ggagcagacg gtatcacagt
     9421 aaacggagtg aaaagcgaaa cttctcctta cagtgtattt aaatgggggg atacagcagg
     9481 acaaggactt tcttatgtcg ctgctaaagc aaatatcaaa gtaacaaata aagatggatc
     9541 tgtggtatta aaagaaaata cagattataa aatccaaaat tcagaaaatg gctttgtgat
     9601 cgattttatc gtgaataatg gccaagtaag tgatactgta gcaagtttac acggacaaga
     9661 tcttcaaatg acttataaca tgtatgtaaa tgacagcgca gctgttgcca atccattaac
     9721 aaatagcgtg gatttcgttt ataacaacaa tccatttaat caggaagaac accacgaaaa
     9781 aacgaaagct gacgttgtga cttatggtgc aaaattctta aaagtcgata gcggactatt
     9841 tggaacagga atcaaagcaa caccgcttga aagcgcagaa tttgctgcta aaaacgcaga
     9901 aggaaaatat tatggtggat tagttgatac ggataaaaac ggtgtcaaag aagcagtatg
     9961 ggtagacgat gtagcaaatg cagcgatcct aaaatcagat aaagaaggtc actttgaaat
    10021 cactggttta actgaaggtg aatattcact ggaagaaaca aaagcgcctg aaaactatca
    10081 aaaattaacg aaagagatct ctttcaaagt agacaaagat tcatacaaag aagaaaaccg
    10141 aattacgatc aaaaacaatc aaaaagcttc cgtaccaatg accggtagca acggttttca
    10201 aacgtatgta ttaatctctt gcttgttgct aggagcaggg gcactttctg cagttgttta
    10261 ctttaagaag aaagcctaga aggatcaaaa ccaatgatta tcaggaagcc taagaaaaag
    10321 cgaatcatgg gtaaatggat cattgctttc tggttacttt cagctgtagg tgtcttactt
    10381 ttgatgcctg ctgaagcgag cgtggcgaaa taccaacaaa atcagcagat tgccgcaatc
    10441 gatcgaacag gaacggcagc agaaacggat tcttctttgg atgttgctaa gattgagtta
    10501 ggcgatcctg ttggtattct tacaattcca agtatttctt taaaactacc aatttatgac
    10561 ggaacgtccg acaaaatttt agaaaatggt gtaggtatta cggaaggaac tggtgatatt
    10621 acaggaggaa acggtaaaaa tcctttaatc gcaggtcata gtggacttta taaagataat
    10681 ctttttgatg atttaccttc agtaaaaaag ggagaaaagt tttatatcaa agttgatggt
    10741 gagcagcacg cttatcaaat cgatcggatt gaggaagtgc aaaaagacga actacaaaga
    10801 aactttgtca cttacttaga accaaatccc aatgaagatc gagtaacgct aatgacatgt
    10861 acgcctaagg ggatcaacac acaccgtttt ctagtgtatg ggaagcgagt aacctttaca
    10921 aaatcagaat taaaagatga agaaaataag aaacaaaaat tatcttggac atggctttta
    10981 ggaagtactg tttttctttc agtcatgatc attggttcgc tatttgttta taaaaagaaa
    11041 aaataaagga gggtaaccaa catgagaaat agaaaatggc tatttgtttg tgcagccgct
    11101 cttttttctt tcactgccta cacacagttg acgtttgcag acagacaaga aagcacggat
    11161 agcacagaac aaacaatcca aacgtcagat agcagcacga ttcaaagcag cgatgaaacc
    11221 gtcgattcgt caacagttga ttcttcagaa gaaccgaaaa ttgctgaaga cgatcaaata
    11281 gtcaaacaga ccattcacgt tcaaaaaatt atttcttccg aacaattaga caaccagggg
    11341 caaaaacttg aaaaacaaga aggagtaaat ggcgcgaagt tttctgttta tgatgtatcg
    11401 gacattttac aaaagatgga tgttaaggat ctcactactg accaaattga atcccaatta
    11461 aaagatcgag tgaaaaaact attcagtgat caattgaagc tagtttctaa tggaaaaaca
    11521 aagacaattg atcaacaaac aggtgtcttt gaattctcga tagaagtaca ggcaaatcag
    11581 aaacaagctt attatattgt caatgaatca agtcctgaaa acatttctaa tagcgaggat
    11641 attttgctat taacacctgt ttctgataaa aacggtgaat tcttaaagga tgtgtggatc
    11701 tatcctaaat cagaagcttc gcaacctaaa gaagaagtaa aaaaaatcgt ttctactggc
    11761 gtaaagaaaa acttctttga gaattgttgg gattttgtta ctcacttgtt ttctagagat
    11821 taaggaagaa ataaaatgaa actgatcaaa ttattattat tcaatttttc ttgtttaacc
    11881 gcagtctttt tgtactgcgg tttattattt tctaaactat ctattttgcc aagctaccaa
    11941 ctatacctaa tgaggatttt aaacatacca gctgcttcca tgccgctaga tattatggaa
    12001 aagctcatta ggaatccatt atttactacg atcaatttga tcgttattgt cggaatttca
    12061 ctagtcattt ataaaaaaga ggggatgaag ggatgaattt taaacactcg tggacaagac
    12121 ggttaaaata tgtttttccc ataatgatga tgttaggtac attgtttggg ttaaaaacag
    12181 ccaatgtctt tgctgaaaaa gttattgtcg atccgtcaaa tccaatccag aacgtaaaga
    12241 ataattttat tattccaaaa gatgttccta atgcaaaaac aaatattact gtaaacggag
    12301 cgtcaaaagt ttacaaatat catgttgatg ccaacaaagg tggttttaca gacgactacg
    12361 taggattagt ggagggaaac aaaaatatac gctatccttc tttccaaaaa gaatacggtg
    12421 aaacaaacct tgttttagaa ggcgtaacaa ccaacaccaa agtcaatatt gactatggaa
    12481 aaatcggtac gtataacggg aaaaaagtca atatcaagct agttctatct aatattcact
    12541 tatactcgga tacgttgccg tggaatctac tagataacaa ctatacaaaa actcattttc
    12601 gagacgatgg ctataaaaat acgaatggtg caatgagtaa atctaaaaaa agaacagtac
    12661 tttggatctc agacaatctt ttctcaggaa ttgtttacca ttcaacacaa atgaatgtgc
    12721 agctagtagc cacatatgag gacggtagcc ccgttcaatt ttcaggagat acatttatca
    12781 gttttaactc tttgaaccct gcaggaggaa aaaccacgga tcttaaagga gaatatgccc
    12841 actatgacaa aatgaatacg acagattggt atgtcagaaa agatacggtt ctttcagaat
    12901 ttaaatcttt ctacaacaat ctgaacgtgg taggtggcca cccaggagga agctctaagc
    12961 tgacacaagc ggataatgat ttcaataatt tgcatgataa attaggagat ccgaagtttg
    13021 gtcaaggtac tgtgtccttt aaaatatctg aagcaaatcc gacgtttgtg attggttctt
    13081 ctaatgttca aacctggttt actttatctt ctgcgacaat ctttagtgtt gttcctgatc
    13141 aacctgaaaa aacaggggtt gataaaaatg gaaataacgt gaacgataaa atgttgcaag
    13201 taggagacac gattcaatat cgaatcaaac agaaggtgaa tcgattagga gtggatttac
    13261 tagcggttta cgatcgattt gagttaatcg ataatttacc aaaagaagta aattacgtcg
    13321 atgcacacgt tgaaagtggt acaaataaaa aattcgatgt atctggtgaa gtgacctatg
    13381 acaaaacaaa acaccaggta aaatatgcag cgaaagctga tacattaaaa aatcgaatga
    13441 agtataacgg cgaaacttac gaattagtga tcaatgtaaa agtcaatgaa ttggccaatc
    13501 aaaatagtgt agccaaaaat cagggaacgt cgatcattaa taaagtagaa aaggatacaa
    13561 atatcattac cgtttatttc cctaaaattc cagtgaaaga agtacaacaa aatggaaaag
    13621 atgtaaacgg acgaaatgac ggtaagaagg gtacgccgac agctccttta aacgcaggat
    13681 ctgaagtaca atatctagtc acacaaaaat ggcacacaaa aggcgtagac gctgcgtcag
    13741 atcactacaa acaatttagt attcaagatc caattgaagc tcgtttgaca tataaagaag
    13801 gttctgcgca agtaatagat aagagtaaag gaaaagatat tacttctgaa ggcacactaa
    13861 cctatgatag caattcaaga acgctgaagt gggaagcttc cgctgacttc ttaagtaaca
    13921 atcttttaga tggacgagaa attcagctga tatttacagc taaaactcca ttacaatcag
    13981 aaaaaaatat tgataaccaa gccgtagtag cagtagataa tgtttctaat aaaacaaatg
    14041 ttgtgacaat tggggttgat cctaacttgc cacaggtcat tgttcctaaa acaggttcta
    14101 cacatttagt aacgatttca gcggtcagct tactattact tgtgttagca acttttagtt
    14161 atgcggttct gagatacatt taaaataaaa aaacgaaaga atgaataaaa tgaaaaagtt
    14221 aataatagta gttacttcct tgattgccat gattgttatt ttagtcggat gtagtaatga
    14281 aaaaaataaa caaactaatc aagataatgg cgtattgaaa tcaggaacta tgtggaaaga
    14341 agaagtaggt ggtcttgttt ataatttgaa aattatagac gagactacct gggaatactc
    14401 tgagagtgta tggcatccag atcctgtaca aataacagtc aagaggcaaa aagactataa
    14461 aggtttagaa agatacaaaa tagtagattc agctggtgtt agggaattta taaacaaatc
    14521 tgattcttta tttattgttg ttccctatga gaagaatggc gtaaaaaaaa taatttttct
    14581 tggatctagc aaggatgaaa agcgaacgaa agagaaattg attcacgatg gatcacaatc
    14641 caataaatat aagttacaaa agactagcga gtgattgcta gtctttttat ttgtcctgaa
    14701 ggaggaatca cagtggcaaa agctaaaacg ccatttgaaa aagccgcgtc taaaatctta
    14761 gaacgccgcg gtgtatctga aacacagtgg aaacaagaag tccttgaaaa agcacaattt
    14821 gacctattac ggggaaaaga caaggactta gaaggatata tctatcaacg tgaacgtgaa
    14881 aagcttgttt tcgacgaaat tattaaacac taaaaggagc gaacaatttt atgtttgaat
    14941 caattacaat gaatttttta acacaaccga aagtagtagc tgcggttgat atttttagag
    15001 cattacaaac tgccgcaact agtttaaaaa acaatctgat gatttttgct gctgctgcgg
    15061 ccgttgcttc cattgttatt gggggtcttt tcttcctatt tggacgaaga ggagctgaaa
    15121 atgggaagat gcacgtaggc tatgtattga tgggggtagg agttatttta gcagcagctt
    15181 ctattgtggt ttcgttattt ggattgtttg gacaaacacc tcaaggatta taagaaagta
    15241 aaaaagtagg agtaagaagt agtcgatact ttttactcct tttttatgaa aggagaatac
    15301 tcatgtactt tacgattgca ggcaatacca aaacagataa agagttaata aaaaaagcaa
    15361 cgaaggaagt aggaaagcgt acttcttttt taccgaaaac aattaaatat tctgtcattg
    15421 aaacggaaga acagctacat gtgttaggga aagtactgga acacgaaggt cgatcttctg
    15481 ttaagttgac tctcacgtta catgacacag aaggaaaaat agagaaagct ggtacagaat
    15541 acgtttttgg cccactggat tatcaaattt cagaaggttt ctattctaca ttgatggatt
    15601 taatccgcct tgatgtgttt aaagaaacgg atgaaactat gagtgtggaa gagtcttatc
    15661 gaaacgaaaa acttttacaa gttttagaag aagagttaga gagcgaccaa agaaaagtga
    15721 atcaagggag tgaagtcgaa gtaaatgaag aagtaccatg ggaacgacca atatttccaa
    15781 agaaggagtc aaaagaaaaa gagacagtct atgttgacga acaagctgat attccgactc
    15841 agaaatcgat atttgagaaa gagaacgatg tgatgggtca gttggaggta gatccctttg
    15901 aagaaacaat ggaggatgaa gagacggccg aagtagaagt tgatccagtt gaagctaatg
    15961 aagagtcaac atggactgaa ggggattctc aggaatatat tgaaccactt atcagtgaag
    16021 aagtagaaaa aaacgaaata gagatacaaa aggcaactgt atttgattat attttggatc
    16081 aatatccgcc agagcatgaa tggatgaaag aaaaaaacaa attattttta aatgagcttt
    16141 attcgaaata tcaattaccc caaacctatc aagcattttt agagagtaaa gaagaattaa
    16201 tctcacaggg taaaggcaca cttttagaac agttagagtc tgtccaatca acaaattggc
    16261 atgagattgc acaagaagat ttattagcca cctttgaaga acgtcaacaa gaaacagatc
    16321 aagaaattaa tgagtatgcg acggaacagt cgtctaattg ggagaaagca aaagaggaac
    16381 taaataaaga agaacagcga gtgattgaag aagaagtaca aaaaattaaa gagcgatacg
    16441 aaaagaaacg cgacaatgcg tattccttgt gtgtagaaaa gatcaataat tttactcaga
    16501 cgaatcaaga agcgttatcg aaagaaaaag aaaacttgtt aaaagaacgt ataagagaac
    16561 gaaaagaaga attttatcaa ggtttgcgta cagataagct aaagattagc aaagaattaa
    16621 atgagcaact gaataagctc tatcaaataa caagtgaagc tcttatggaa aaacacgagc
    16681 agatccagca agaattacaa gtacaaattc caaaatggaa aaaagagtat gaaattcaac
    16741 aacaagaatt gaaagaacaa gaagaaaaac aacggcaaaa agaaaaagag cgcgaacaat
    16801 tagaactaaa aaaacgtgaa caggatcttg cggaacaaca aatggcaata gaaaaacaac
    16861 ggttaaacca agaaaaggaa aaagaagcag ctgctcgtaa ggaacgtcaa gaacaaatgg
    16921 atacattacg acaagctcag ttaaattatc tagcacaacc tcaaccaacg ccttcagtag
    16981 ctgctgttca aaccccaatg aacaaccaaa caaatctaac tccttcacca cagcgttcct
    17041 ttaacggatg gatggtggga tgtattgcaa gtttaagctt gcttctaggt ggagggacag
    17101 tatttgcttt taatcatatc aatcaatctt cacaggctga ttctgtagcc acaacggcgt
    17161 caatcaagga agaagcccaa gaacaagcaa gaaaagagta tgaacaacaa ttggaagaat
    17221 tattgaatcg taccaatcag gcaacaacag agtcaagtat ggaaaatagc aaagagagtg
    17281 atcctagtaa ggctaaagaa ggatcaagcg attcaactgt acaatcaaac acttccgaaa
    17341 gttcagaatc aaatgaggtg aaataatgcg tgttagagaa acaacgagac gaaaagacaa
    17401 acgattcaat ttagaaaaga ataggtatct ttctcatttc tatcgctata ctccttcagg
    17461 agaagagcta ccagaaaaac atgacaaacg tatttctcaa aagatttttt atagtggctt
    17521 agcttatctt cctttaaact atgttgttac aagaatacca gaagcatttc atcgatttga
    17581 gattattcca ggaagtttac aaggagtgaa tatttttcag ttacaagaaa tgccgcagtt
    17641 attccatttt gattcaactg gtttttggaa tcctttgacc ccaacaacaa gtttaggcac
    17701 acgtttagtt gtttctacga tttgtgttgt tccagctata cttatgggat tgaaaatgga
    17761 catgtggttt cgtcctctag cagatgggca aaaaggagat aaccgattaa aaacgttaaa
    17821 ggaaatccaa caaatttatc cgcaaatacc agaatcaaag ggaggttttc ctggttatgg
    17881 cggaatacca attactcatt ataaaaagta ctggtatatt cagacggata cagtaaatac
    17941 ttgtattgtt ggtacttccc gttcagggaa aggacagata gaagttttag caaccattga
    18001 caatttgtct cgtgcagaaa gacaaagttc aatggttgtt aatgatccaa aaacagaatt
    18061 atatgttgct agtaaagatg aactagaagc tagagggtat gatgtgtatg catttaatat
    18121 caccgatcca ctgcaatcaa tgagtgataa tccattggca ctcatcgtga aatactggaa
    18181 gcgaggagac atagatactg caacgcagtt aaccaatact tttacgaata cgatttatta
    18241 tgatgccaat gcttcagaca acaaatactt taacgaaaat gcgcagaaag ctgtaaatgc
    18301 gcttattttt gcgttgttag aacaagccga tcaaacaggg gattactcca aattaacacc
    18361 caataatctt gttgagcttc tttctgaaat tggcggtttt aattatcaag atcctgagaa
    18421 tgattacaga caactcaatg ccttagatga attcatgaat cagttaccac ctggaaacat
    18481 agcaaaaaaa caatatgggg caacaaaaat tggttctgat aaagcaaaag ggaatatttt
    18541 atcgacggca atcacaggat tgaatccatt taccctaacg aaaattgcga aaatgacttc
    18601 tcaatcaacc attgattata agtccattgg ttttccgaag tatttggatc tcaaactaca
    18661 cgagagtctg ttaaatcagc gaattgttgt agagttttat catcaaggga aaaaatttca
    18721 tgaggaacgt gtcaaagtag gttatagagg tttttgtgaa atcaactttg attgtcattt
    18781 agagcaggga gatcatgttt gcttaaaata cgatcatcaa ggaaaagaat atgttgcttg
    18841 gtatgagttt tctcaaagag tattgaaaga cgaagaggga aatatcgttt atcgaaaaaa
    18901 acgaggagaa acccatttac cagaattaga aaaagaagct gttctaacat tagatgagga
    18961 taaaagtaac ttattagtag agtctattcg gatgcactat accgataaac caatggctat
    19021 ttttatgctc acacctgatt atgatccatc taaccatatc attgttagta tttttcttag
    19081 tcagttgtat aaagaattat ctacgcaatg tgttaagact aaaggagata aatgtcaccg
    19141 taggattcat tttattttag atgaatttgg gaatatgcca ccaatggata atatggaagg
    19201 aatcatgaca gtcacagctg gtagaaacat gttgtgggat ctgtttattc aatcctatca
    19261 gcaactttat gcgaagtatg gggaacaaaa cgcagcgatt atcaaagaaa attgtcagac
    19321 acatatttat attatgtcga cagacgataa taccatcgaa gaattttcca aaaaagtggg
    19381 gaataagacg gttgaacaag agaacagtac caccaattca ttaggtatga atactcatat
    19441 caatcgcaat gttgattcag atcgtatctt aactcctgaa agaatttcta cttttttaga
    19501 aggtgaaacg attgtactcg ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg
    19561 gccgtatcct atttttaatc atggggaaac gaatcttcct tatgcttatc aatatattga
    19621 agatttcaat ccatcgtggg atatcaatga tatagacata cattgtacac atgcggactt
    19681 ggatcttaag gctttacaaa ttgattacga ttattttcta cctgatatct atgcacgtcg
    19741 actttatcac aatatgcacg atcaaataga agaggaagct aaagaaaaca tttttattaa
    19801 aatcgttcaa tctttagaca actcaacggc acaaaagtta cttattcaat tttatgaaga
    19861 gaagcaaata gatcgtttgc aagaatacct tgaaaaattg catcaaaatg aagaaattac
    19921 agaggttata aggaaacgac ttttgctaga agctgaaaag gcggtgaaag aatgatcatt
    19981 ggaggaatat tttccggcat tgccaactgg tggtcaggct tatttgggga tggggatgca
    20041 gatcccaaaa ccgtaacgaa agtcttacaa gattatggta attatctgca gtatcaagat
    20101 gtattcagtt ggatttgtaa tactttttta tggggtttga ttaaagggtt atataatctc
    20161 aatactttac tagaaaaaac aatttatcaa agctttgatt taaaagactt attgaacgct
    20221 gctggtgtta atgaactgtt tcaaagtttg attagtaagg tattggctct tttttgtgtc
    20281 gtcacattaa tttatttagg cttaaaattt tttatgagta aacatccacc aaaaatcaaa
    20341 aatatcttgg ttaatctatt tatggccatg attttgattt ttggtggtag ttctttaatc
    20401 gatgaagggc taaatattag taaaaacttt tacggagacg tgactgcagc aaataaagca
    20461 gataaaactg gatcacctgc ttttcaaatt attcaaaata atgtgatcga tgtgagtacg
    20521 ctactagaaa ctgatccgaa taaagtagaa tccctaccaa cagataaaag aaatgctctc
    20581 acggcgaaaa actttaagac agcaaatatt aattcagtga ttattcctaa ggaagccgct
    20641 aagatggcgg atgaagataa gagcggtcta tcagatatac aaaaggatcg gctaagagcg
    20701 ttacaatacc gactggaact ggatgataat ggaaaagaaa tccctgtaga gattagtgat
    20761 gaggggttag caaaatatgt gtatacttca ggctatcgcc gatacattag tagtccaggg
    20821 attattttag ctggtgaatc ttctttgtcg gttgcctatt tatttatctt gtttacgatt
    20881 gtaacttgca ttattgagct ggtatttaaa aaattctatt tagtaattgc cgcgtcaacg
    20941 gactttgaga caggacaacg gatgaaaagt gctatagaag atatttctca atccttcttg
    21001 ttattggcgt ttacgatttt agaattacga atctatacat taatgctgtc aggtatcggt
    21061 gatttgcacg cagcaggcaa gatcaacgga tttttgtatg ttgtcgcttt aattgtacta
    21121 accattgcgc tattcaaagg ctctcaatct gttacgaaga ttttcggtgt tgatacaagt
    21181 ctgaaaaacg gcagcaattc tcttcttagc atgtttgcct taggaaatat tgcgaaaaat
    21241 gtgggtagtg gggctaaaaa ccttgcaagt gctggtaaga gtggtttaag caatttaaat
    21301 gatattcgta aaaatggatt tggcaatcga aaagatgtta ttcctgataa cggggaagaa
    21361 caaccagacg ataaagctac aattgtacct aatagtaaga aaacagtttc cgaaatgttc
    21421 gggaaagcga aaaatggtgt ttctaaagcc gcagaaggtc ttggttacgt caatgaacgt
    21481 gggttaaaag gaacagctga agatcttaca gaaaaagcca aagacacctt agaagagaaa
    21541 ttagacggca cgagagctaa agaattggct gaagctgtga agaatccatc cgaagcacta
    21601 gaaaaacaaa aagaaaaagc ttcagaagtc aaagaaggac taaaagatgc gtatggtgaa
    21661 ggacaagtca atgcagcagt gaaggcaaat acaacaagtg aaaaagaaaa tcaagaaggt
    21721 gcagcgattc cttcagccaa agatcaccaa gatgagttac attctaacga aaattcgatc
    21781 ggagaaaaag attcagaaga aggacaacat ggacagttac caaaaaccga tccagaacaa
    21841 tatcgttata aatctaacat taattctgaa aatgaaaagc gcatttcaga tttaccaaaa
    21901 cgaaatcaag aagtacaaac ggatgaggat tccatcgatt tgccaaatga taatggaatc
    21961 gaagttggaa ctgaaaaatc aatgaaaatt ggtttgccta agacacaaaa tttaccaatg
    22021 aaagatagtc gtttgaatca attcaaggag aatgatcaaa gctttgttca ccaatcaact
    22081 gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct gacaactgtt
    22141 tcttacaatt tatctgaaag tggtgagaca gggaatgtgt ccattcaaaa tgttgtgaca
    22201 agagaagctg caccagaacc tattcaaaaa gttcacacag gttacaaacc agcaaatgtg
    22261 gcatttactg caccaacaaa aataaatgta acaagagaaa ctaaaaacag gaacaatgaa
    22321 ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa ggcctacgag
    22381 ttaggccttt tttctatgta tgaaaaggag aaatcattta tggataaata tattaacatg
    22441 aaaaatcctc caagatttag tggggaatgg aagtttttcg cctttttctt tttagatcta
    22501 ttaacgcttg gtggttgctt tttttgggcg aaaatagtca ataatcttgt ccacttacca
    22561 ctcttttttg cactcttgaa ttatggctta tggattgcga tagctctagt gattgtttgg
    22621 cgaccttata ataacccagg tcaacgacaa atttttgcga ttatcgaatc tattttcttt
    22681 gcggataaga accactatca tgcgttagat cctaaccgaa attatcatgg tacgtataag
    22741 gaggatgaat aaatgcgagt caaaggacaa aattcagttg ataagaaatc cattttgcag
    22801 tttggaaaac cagaagaacg atctatcttg gatttatttc cttataaatc ctgtacagaa
    22861 gagggagtct tagttactaa agaagatcat tttcaacgtt attatcgtgt agtttctaca
    22921 gacgtggaag gattaaacga acaagagaaa ctagaacgaa tgaatcagtt gaccaccgtc
    22981 atgcgtacgt atgtgcaaac aattaagtta gttagtttga ccacagagac ggatctaacg
    23041 gaacaaatcg tacaaaagcg gcaattatta gataagaatc gtatggaaca atccttagga
    23101 agaaatctac agcgattaag aaaatatgaa aagaaattag tagaggaaat ccaggaattg
    23161 aaacgagcag aacaagaacg tcctgactta agcttctttt tcgtcattga aggaaagaca
    23221 atcaaggatt tacaaaccag aaatcgtcag ttaatgcgat ctagtggcat tttagggtta
    23281 aaaccactct acaaaaaaga attgatcaag attctttatc gaatgaacaa tatgaatgat
    23341 gagtaggaat ggagaagaga gaatgaaata tccgttgata tggttagaaa atgatgttcc
    23401 gaaggaagag atcactagaa aattttctca cactttaagc ctgggtaagt tgggaaaagg
    23461 gcaatctttt aaccaagtaa agaatcaaac aagaagaggt gactgtacat gttagaagcg
    23521 attaaaacag ctttttcacc acaaaatgat caaaaagtac ttgatccatt aaagcaacta
    23581 ggaaatcggg aaatagaaat tttaaaatcc aaagggtatg atttagattt tttaaaacaa
    23641 gtgatgccac aaggtggatt aaatttcgat gaagagtatg tgacctatgg aaatggttat
    23701 tcacgatgtg tgacagtcta tcactataca aaaaatccta ctcttttttg gttggccaat
    23761 ctcatgaaca atcctcatac agtggccaca ctagatgtta gcacagatga aaagcaaaaa
    23821 gtgattgatc aaatcaaccg cgcattagat gaattaaatg atcgatcaga taatgaacga
    23881 aaagcgactg accgaaataa aagtgcggaa gaatacctgg aactagcgga atatgcccga
    23941 aaattgactt ctaatgggga aatttctaaa caagtcaaag tgagagtaca tgtttacgga
    24001 aatacgatac aagaagtgga tgaacgggcg caggaactga tcgaaaatct aaaagggatg
    24061 gattatcgtg cggtgactta tctgcataaa accaaacgag attggcaatc gatgtttacc
    24121 atgttcgatg aacaagctga atggttcgga aatatcaaag ggcaatcact accatcgtta
    24181 aacattggcg gaggtttccc gttccaccac caaacgctaa aagatccagg aggtacacaa
    24241 tatggggaaa ccatgacagg cggcccattt gtctggaatg cgacgtataa aaatagtgta
    24301 cggttgtcta ctaatacctt gcttctagga atgatgggag ctggtaaatc gaccgttctt
    24361 aaaatgatcg cggaagccca tttagctgca ggagacttta tttggggctt tgaaaaggga
    24421 aaagacttta ttcctttctt aaaggaatat aacggtatta tggtgcgttt agacggatct
    24481 gacgggatga tcaatccttt agagatattt gccacacgga cgtatgatga agcatccagg
    24541 ttatatgacg atggatcagt caaagatttg aagatcaatg aagcagcgtc ctatcaaacg
    24601 cacttagaca aagtagtcta tcaggtacag ttagtgagtc ctcagctaaa aggaacgatg
    24661 aaagcagaat ttaagaccta cttaaatcgt ttttatgaag agtatggaac agtaccacgt
    24721 ggctttactt cctcaaattc gcgttcaaat actgatacgc aagtcacagg taaagatcca
    24781 gaagcttatc cgacgttcaa agaattttta gactttttag gacaactgga attaccaggt
    24841 gcttcccaag agaaaaagaa tcgaaaagaa gaaatggaat ccatcgtaga atccttatgt
    24901 gagacgtatg gcatgatttt tgatggtcac agtacgattc gacaccttga ccaacaacag
    24961 ttggtctttt ttgacattga ctctattaca ggtttagatc gaggggtgca acaatgtcaa
    25021 atgtacatgg ctatgacgtt gatttggaat caggcattga tccagggaag aagacaacga
    25081 cgtttaatta aagaaaaaca aatggaagaa caagacttca aacgcttctt ggcgatgatt
    25141 gatgagtgcc ataatggtgc gacctgttct gcagtgaaaa gctgtgggtt gcaagtagtc
    25201 taaaaactat tgtaataact cttataacga gaagtcaaat gagtgggtaa cgccacaaag
    25261 ggcttccact gatactccga catgcaacaa gtagtttatg cagaccaacg ttgtgtgaag
    25321 ctcggtgaag tcggctgaag gataccgtaa ggtaaaagaa atagtgaata ccacgaaagt
    25381 tctttagagg taaagggtgt atccgataag tcgggtgtcc aaaaaaactt aatatggtga
    25441 gtatgtacaa acgtactgac gaactagcga atgtactgtc cgaaaggcat agctcctaga
    25501 aatgggagag acagtgagca aaaaatcact cattgtgcta acttgtggtt gagtaaaagt
    25561 cgttgttatg aaagaccatg ctgcgttaca ggcgtaggag aagttagggg gcaaaagcta
    25621 gcatctaagt taaggcatgt ggatagatat aagggaacag gggaagccat caacatggag
    25681 agtgcatact cgatgaagtg gtagtaagaa atatgtgaat gacaaataac ttactttagt
    25741 gatggtgaaa gtagagtcac gactgatgaa ggttctgtaa tggaactgga ggaacaggct
    25801 cgattcgtct gtaaaagtca tgaaacccgt tcaaccatgg gattctcgta tgactaaaaa
    25861 ggtcacgagc caattggagc gtgataaacc atgacaaaag aaacaagtcc aaaagatagg
    25921 aagttgcggc atgcagaata ttatggcatg acggaaatct tcgataagat atactccaaa
    25981 agtttgaagg gagataattt caagaatcta atgcagatta tcatttctga taacaacatt
    26041 gtcctagcat atcgtaatat caagagaaat cacggtagtg tgacaagagg agaagatgga
    26101 gtaacgatta aagatattga aaatctcaac cgtaatgaat tcgtagaaat tgttaggaag
    26161 cgattcgcac attataaccc tcggaaagta aggcgtgtag aaattcctaa atataatggt
    26221 aaattaagac cgttgggtat tccatcaata tgggatagaa tcgcgcagca atgcatctta
    26281 caagtattag aaccaatatg tgaagcaaaa ttcaataatc acagctatgg tttccgccca
    26341 aatagatcag cagaacacgc aatagcagat tgtatggccc gaatgaatca aagtcacatg
    26401 gaatatgtgg tagatgtaga tatcaaaggt ttcttcgacg aagtcaatca tactaaactc
    26461 atgagacaaa tctggtctat gggtatacga gacaaacagc ttctcttaat cattaggaaa
    26521 atgttaagag cacccgtagt acttccaaat gggaagttga tatacccaac aaaaggaaca
    26581 ccacaaggcg gaatacttag cccattactt gcgaacatca atctcaacga atttgactgg
    26641 tggatagcaa atcagtggga ggaaaaagat gccaaagagc taaaacatca ttttaggaaa
    26701 aatggagctt gggataaagg taatatctat cacaagctca gaaagagtac aactctaaaa
    26761 gaaatatacc tagtgagata cgcagatgat tttaaaattt tctgtagaaa ttataatgaa
    26821 gcgaaaaaag ttttctatgc aactaaaatg tggttacagg agcgtctaaa gttaccaata
    26881 tctgaagaaa agtcaaaaat cacaaatctt tcaaaagaag aaagtgaatt tttaggcttt
    26941 acgatgaaat tagtgaccaa aggtaacaaa aaggttgtta atacgcatat aggtacaaaa
    27001 gcacaaagca tgattaaagt gaaactaaaa aaacaaatca aacgaattca aaaaagtcct
    27061 aatagcaaag atacctttat ctatattgga gtctataaca gtatggttat tgggattcat
    27121 aattacttca aaattgcgac tcattgtagt gtagcaatgc gaaaaattgc aagagaactc
    27181 agaccaagtt ttgaaatacg actacgagaa caaggaatct caaaagaagg acaatacgga
    27241 aagtacgaag gatacaagcc ttacctacgg tcaggacaaa tacgatatat aagagggttt
    27301 ccaatattac cagtagggta tattcaacac agacatccga tgtgtaaaaa aagaaatatt
    27361 aataaataca ccgaagaagg tagggaagcc attcataaga agcaacaaac cgtagaagaa
    27421 tggaagatat cgtggttgtg tagcaatcca gtgcttagta agagagcaac gattgaattt
    27481 gcggacaatc gaatttcaaa attcatcgct caaaagggaa gatgtgcagt aacaggtgag
    27541 gagcttatct tatctgaaat gcattgtcat catattatcc cttatcatga aagcaaaagt
    27601 gatagctatg aaaacctagt gattgttact gaggaggttc atcgggtaat acacgctaca
    27661 caaagtgaaa cgattgagga acttctaaaa tatctaaaat taaatccaaa acaaaaagaa
    27721 aaactcaatg agttgagatt aaaagttggt aacgaagaaa tatcttaata aatatttgta
    27781 ggacgggaaa acagaatgtc ttttatagac gagggaacgc cgtatgcggt gaaagtcgca
    27841 cgtacggtgt ggactggggg aaaagacgga gataatatca aactcttacc tatcagtatc
    27901 gattaatcca gattttattg aaacggtaaa ttatgtcaca aactttcaac gagaaatgcg
    27961 taaagtagaa gcaatgactg ttttagctac acaatcccca caagaaatgg tgccagaaaa
    28021 tatgtcttct gataacttct cgaaactgaa gaaagtcttt gaattttctt ctacgaagat
    28081 ctttatgcgt atggatgaat ctattttacg tcatatagaa ggactagtgg gtaaatcaat
    28141 gacgaactca gaattagaat ccattcctca acaaaatcaa ggagacgctg tgatcaactt
    28201 tggatcaaaa gaaaccattc gcgttcattt tagtccgaac aaacgtcaat taaaattgtt
    28261 tgatggagga aaataatgga aaatcaagat gaaagtttaa tcaagcagta tgttaagcgt
    28321 cgcgcaaagc ggcgcttatt tttgtggttg tttggtactt ctgcaggttt gattactatt
    28381 ttgatcactg tgtttgttac cctatttcta atacttgcgg cgggttcgat tgataattcg
    28441 gattcagatt caagcagtgg tggcgaagct tttacaggag aatattcgga aggcttaccg
    28501 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgccgta
    28561 ggtgcagcag tgaaatataa attattacca agtgtgattc tttcgcaata cggttatgaa
    28621 tcagcgtttg gtacgtcggc gtccgcaaga aatgatctca actattttgg gattacttgg
    28681 tttgatggtt gtctgtttcc taaaggaact gcacgaggaa ttggtggcat tgaaggtggc
    28741 tggtacatga agtttcccaa ctcaaaagct gcattctcct attacggctt tatggtagcc
    28801 acacaatcaa attttaatgc atgtgtagga aataaaagcc ctggcgcaag cttattgatc
    28861 ttaggacgag gtggttatgc cgctgcagga attactgaag atagtccgta ttatacaggt
    28921 tgtatgtcga ttattacctc aaataaatta acagaatatg atgaatttgc gatcaaacat
    28981 tggggagaag gcggaaatga caacggtacg attactggtg aatggacgaa tcctttccct
    29041 ggcagcagtt tagataaaag ttccttcagc ggtggtcaat tatttggaac gaatccaggc
    29101 ggtgaattcc gtccgaatgg tttccatgat ggattagact ttggttcagt tgatcatcca
    29161 ggaagtgaga ttcatgcggt tcatggagga aaagtcgtgt acgttggaaa tcctggtatt
    29221 tcaggtttgg gcgcgtgtgt gatcgtgatt aattacgacg gcttgaatat ggtatatcaa
    29281 gaatttgcaa atagtacagg aaattcccgt gtgaaagtgg gagatcaagt gaaagttggt
    29341 caagtaatcg ggatacgaga tacagcacac ttgcatttag gcttcactcg aatggattgg
    29401 cgtcaagcac aaggacacgc attcacagat gatggtacat ggatcgatcc attaccattt
    29461 ttgaatagca gcaaaaaata gatcaggaga aaaaatatgg aaaataaaac gttaaaaatc
    29521 gtcttaacag ctgcactcag cattagctta attggcaata tcgcgctctt ttggcagctg
    29581 aaagaggaac aagcgacaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgag
    29641 aagctcaaaa aaaatttctc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
    29701 aatgagaaaa accaagaaat cgatcagctg aagcaacaag acggtataaa agcaaaagaa
    29761 gaagtcacag atgcccaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
    29821 tcactaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
    29881 gacaaattaa gtcctgcaga tgatgatcac caacatgatg attatggaag ttactccttt
    29941 acattaggtg acgtgcaaac ttacgctgaa acaagcagtg taaaagagga acaaaatttt
    30001 gtcgtatttg ttgattatac aatcggcaat cctcaattca aagatgtcaa accgcaaaaa
    30061 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcacagac
    30121 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag caagacaacg
    30181 ttttaaaaaa cggaaaagtt atcttagatg gctgggaatc tttacgattc tcggcttttt
    30241 gattcttgct agtccgattt atacgcataa gaaggtaaac ataccagatg aggtaagcac
    30301 gaacgctttt ttaaacacga aagaactgac aatggtatca aaagaaaaag gagcgaatca
    30361 gcaattagtt tttgaatttg cgattgacga tgaggatcaa ggcttattaa aagaactggc
    30421 taatcttgat tatcgcgtag aagtaaagac agctaaaggt gattatcaag caataaaagc
    30481 caaagtgatc aaggtgtctg atgattattt tgtagtgaaa ttagcacatg taccagaaga
    30541 atatatcgcc atgcgtttaa ccattgttcc tgaaaaaatt gatccaaaag tggatatgca
    30601 agaaccacaa gatctgattt attacgttca cgaagataag gttaaggatc aagtgaaaga
    30661 tgataattat gaacaacatg cgatcaatta caaagtgaaa ggatacaaga aagaacaaaa
    30721 agcagcacaa aaacagattg aatccttgca agcaaccatt gatttgaatg aagaattagt
    30781 gaataaacta aaagaccaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
    30841 taagattaat gattaccagc aagaaattga aacatcgaaa acgcaaatga aagatcaaga
    30901 agagatcatt caaaaaattc aagaaaaaat tgatcgtatg acaaaagaaa atatttagta
    30961 gaacaggtgg agtgaaagaa atgaagggag cgaacttggt cggcaaagat attttcgctg
    31021 agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcaag
    31081 aagtgaattt atctaacaca acaatatctg agttgaagaa tggtagaaag agtttagata
    31141 ccttaaaaat cagatcattt gaagctttgt atttagctgc taaaaaatat tttacggctg
    31201 aaagaaaata aaaacaggag gattttatgt g
//
LOCUS       DAZKSU010000029        30479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000029 DAZKSU010000000
VERSION     DAZKSU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.029"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..456
                     /locus_tag="ACG8Z3_001351"
     CDS             <1..456
                     /locus_tag="ACG8Z3_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0823672.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGL
                     PKEMDFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLT"
     gene            complement(512..2875)
                     /locus_tag="ACG8Z3_001352"
     CDS             complement(512..2875)
                     /locus_tag="ACG8Z3_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFQ0823673.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            3040..3544
                     /locus_tag="ACG8Z3_001353"
                     /pseudo
     CDS             3040..3544
                     /locus_tag="ACG8Z3_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3652..3999)
                     /gene="rbfA"
                     /locus_tag="ACG8Z3_001354"
     CDS             complement(3652..3999)
                     /gene="rbfA"
                     /locus_tag="ACG8Z3_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0823674.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(4026..6332)
                     /gene="infB"
                     /locus_tag="ACG8Z3_001355"
     CDS             complement(4026..6332)
                     /gene="infB"
                     /locus_tag="ACG8Z3_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0823675.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(6345..6656)
                     /locus_tag="ACG8Z3_001356"
     CDS             complement(6345..6656)
                     /locus_tag="ACG8Z3_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0823676.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6653..6946)
                     /locus_tag="ACG8Z3_001357"
     CDS             complement(6653..6946)
                     /locus_tag="ACG8Z3_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0823677.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6968..8143)
                     /gene="nusA"
                     /locus_tag="ACG8Z3_001358"
     CDS             complement(6968..8143)
                     /gene="nusA"
                     /locus_tag="ACG8Z3_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0823678.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(8167..8640)
                     /gene="rimP"
                     /locus_tag="ACG8Z3_001359"
     CDS             complement(8167..8640)
                     /gene="rimP"
                     /locus_tag="ACG8Z3_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0823679.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8784..13136)
                     /locus_tag="ACG8Z3_001360"
     CDS             complement(8784..13136)
                     /locus_tag="ACG8Z3_001360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0823680.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(13348..15057)
                     /locus_tag="ACG8Z3_001361"
     CDS             complement(13348..15057)
                     /locus_tag="ACG8Z3_001361"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0823681.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(15125..16393)
                     /gene="rseP"
                     /locus_tag="ACG8Z3_001362"
     CDS             complement(15125..16393)
                     /gene="rseP"
                     /locus_tag="ACG8Z3_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0823682.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16554..17354)
                     /locus_tag="ACG8Z3_001363"
     CDS             complement(16554..17354)
                     /locus_tag="ACG8Z3_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0823683.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(17351..18163)
                     /locus_tag="ACG8Z3_001364"
     CDS             complement(17351..18163)
                     /locus_tag="ACG8Z3_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0823684.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(18358..18915)
                     /gene="frr"
                     /locus_tag="ACG8Z3_001365"
     CDS             complement(18358..18915)
                     /gene="frr"
                     /locus_tag="ACG8Z3_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0823685.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18918..19640)
                     /gene="pyrH"
                     /locus_tag="ACG8Z3_001366"
     CDS             complement(18918..19640)
                     /gene="pyrH"
                     /locus_tag="ACG8Z3_001366"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0823686.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19776..20657)
                     /gene="tsf"
                     /locus_tag="ACG8Z3_001367"
     CDS             complement(19776..20657)
                     /gene="tsf"
                     /locus_tag="ACG8Z3_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0823687.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20756..21538)
                     /gene="rpsB"
                     /locus_tag="ACG8Z3_001368"
     CDS             complement(20756..21538)
                     /gene="rpsB"
                     /locus_tag="ACG8Z3_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0823688.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21897..22376
                     /locus_tag="ACG8Z3_001369"
     CDS             21897..22376
                     /locus_tag="ACG8Z3_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0823689.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22593..23684
                     /gene="phnW"
                     /locus_tag="ACG8Z3_001370"
     CDS             22593..23684
                     /gene="phnW"
                     /locus_tag="ACG8Z3_001370"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0823690.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            23677..24462
                     /gene="phnX"
                     /locus_tag="ACG8Z3_001371"
     CDS             23677..24462
                     /gene="phnX"
                     /locus_tag="ACG8Z3_001371"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0823691.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24808..26499)
                     /gene="argS"
                     /locus_tag="ACG8Z3_001372"
     CDS             complement(24808..26499)
                     /gene="argS"
                     /locus_tag="ACG8Z3_001372"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0823692.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26921..27868)
                     /gene="arcC"
                     /locus_tag="ACG8Z3_001373"
     CDS             complement(26921..27868)
                     /gene="arcC"
                     /locus_tag="ACG8Z3_001373"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0823693.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27983..29002)
                     /gene="argF"
                     /locus_tag="ACG8Z3_001374"
     CDS             complement(27983..29002)
                     /gene="argF"
                     /locus_tag="ACG8Z3_001374"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0823694.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(29093..30322)
                     /gene="arcA"
                     /locus_tag="ACG8Z3_001375"
     CDS             complement(29093..30322)
                     /gene="arcA"
                     /locus_tag="ACG8Z3_001375"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0823695.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         8172 a         6551 c         5422 g        10334 t
ORIGIN      
        1 acgattgtcg gcatccacca taaaagtgcc gtcatcacac tcgttgagcg actttcaaaa
       61 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
      121 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      181 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      241 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaagat
      301 ggactaccaa aagaaatgga cttcaaccaa gtcaatcaag gattcatctc atccgttgcg
      361 tccaaaagaa accatatccc tagaaaatca ctaaattacc aaacaccatt agaagttttt
      421 ttgagttacg taaatggaaa gttttgtctc acttaatttg acaaataata caatgtaaaa
      481 aattctgttt tgcgggatcg attttatcat tttattggtc aagatttttc catttccaat
      541 ccaatacttc tggcagatca ataccatttt cacgaatata gttttgatgt tcttgtaatt
      601 tctgatccat tttttccgca aaatcttctg cttgacttcc tttgattttt aaagctgcgt
      661 cttttgccaa atgataacga tcaagttcat ttactacacg catgtcaaat ggtgtcgtga
      721 tatctccatt ttctttgtag ccatgaatca ttaaatgatg gttatgacga tcaaagaaaa
      781 tcgtgcggac caatgtttca taaccgtgga aagcaaataa gatcggtttg tcttttgtaa
      841 aatattgatc aaattcttca tcgctcaacc cacgcggatc ttgacttgga tgacgtaatt
      901 tcaataaatc tacgacattg atgaaccgaa tttttagttt tgggaattct tccagcagca
      961 aagttacagc tgctaatgct tctaagttag attccgttcc tgcagcagca atgaccaatt
     1021 caggttcctc ctcttttgct gtcgaagccc aatcaatgat tttcaagcct tcattcacta
     1081 agacggctgc ttcttctgct gagtaaaatt gagcacgcgg atgtttggaa gagatgatca
     1141 aattgatttt ttctgacgag cggaatgctt tatccatgac tgcgagcaat gtatttgcat
     1201 ccgcaggcag atattcacgg atgaactccg ccttcttttc tgccaaatgt gtcaatattc
     1261 ctggatcttg gtggctatat ccattgtgat cttgctggaa gactgttgag cttgcaatga
     1321 gattcagcga aggataatct tttcgccaag aaaggtcatg tgatttccgt atccatttga
     1381 agtgctgagt caacatagag tcaaccactc gcaagaatga ttcgtaactt gcaaaaaaac
     1441 catgccgtcc agtcagaacg tacccttcta agaacccttc atcttggtgt tccgataatt
     1501 gactgtcgat gacgcgacca gatggcgaaa gccattcatc attttctggt tcgatttttt
     1561 ccatccattg gcgatttgtc gttttgaaaa cttgattcaa tcggtttgat tttgtttcat
     1621 ctggtccaaa tatacggaaa ttttctggat ttttctcaat gatctcgcga acgaatttac
     1681 caagttcggt catatcttct gctttaacgg agcctggttt ttcaaattgc aaagtatatt
     1741 ttttccaatc tggcataata agcggtcttg gatcgattcc accattggtg ataggatttt
     1801 ttgccattct ttggtcacca gtaggcgcaa tttctctgat ttcttctaaa atacgtcctt
     1861 gtgcatcaaa taatttttct ggttcatagg atttcagcca acggagtaaa gcatctgcat
     1921 gatccatatg ttcttgatct acaggaatcg gtacttggtg tgctcggaag gttccttcaa
     1981 ttggttcccc gtcccattct tttggaccgg tccatccttt tggtgtgcgt acgatcaata
     2041 ctggccattt tggcatcacg acatctgttg ctgtcttcag gcgagcttct ttttggatgg
     2101 atttgatttt ctcgattgct tgatccattt tttcagccat aacaggattt aatttttctg
     2161 gatcatttcc ttcaataaag aaaggctccc agccaagtcc gttaaagtaa tttgctaatt
     2221 ctccatctga tttacgtgcc aagatcgttg ggttggctat ttttgcacca tttaaatgaa
     2281 ggatcggtaa aacagctcca tctatcactg gattgataaa gacatttgag aaccagcttc
     2341 cagctaaagg accggtttct gcttcaccat caccaatcac gacagcagag attacttcag
     2401 gattgtctaa tacagctccg actgcatgtg aaagagagta tccaagctct ccgccttcat
     2461 ggatagaacc tggtgtttct ggtgctgcat gagaagcaat gcctccagga aatgaaaatc
     2521 gcttgaataa tttttgcatt cctgtctcat cttccgttac ttctggatag atctctgtgt
     2581 aacttccatc taaataagca ttcgatacca tgacttgtcc gccgtgtcct ggcccttcga
     2641 tataaaacat gttcaaatca tatttattga tgactcggtt caaatggaca taaataaaat
     2701 tttggccagg aatcgttccc caatgaccga ttggatgttt tttgacatct tctggtttca
     2761 gtgtacgtct cagtaagggg ttatctttaa gataaagttg cccaacagaa agataattag
     2821 cagcacgcca ccaagccgtc attttatcaa agtattcttt actagaataa tccatacata
     2881 acactccttt tcattattac tcttattcta gcgcagatac tcccaattga aacctaataa
     2941 gcacttctgt tcatttgttt ggattttata tctagccaaa tgaacaggta taaaaaacca
     3001 cgaaattttt cctttcgctg acgtttttta cattgatcca aaaaacttgt cgttatcaca
     3061 atggctgttc cgcagttcat ttcgctcctc atcatgcata atttgctgaa tgacgctagg
     3121 ccagttaaca cattgttgga aaactggggc tggatcgagc aattcctttc ctgattgatc
     3181 cgttttatgt aaaagtctct gttatctttg tcaatatgtg aattggtatc ccagctacta
     3241 tgctaatttc tacagggatt atccaaaatt tggatcgtga ccaaatcgaa gcggcgtaat
     3301 cggaaatatc aataacttca acgtgatttt tcttttaact gtcaacggac cgacaaactc
     3361 aaacttttat ggtgcaggtt ccactgatct tctagttact tggctatacc tgactgtcac
     3421 taaaatagat tacaatctag cttccgttat cggtatcttg atctttatct atatgcaaaa
     3481 atttatatac tatagaaaaa agaaagtcga attttttact tttctttttc tcttttttgg
     3541 ttagctgaca cgctaccaga ctaaagtcat ggaactctta ggtaatacgt cttaatgaaa
     3601 acaaaaaaat cccgcaaccc ctgatttcac aggatttgcg ggattttttg attagtcttt
     3661 gttcaagtcg cgaagcaatt catcgatatg gttcccatag acaactgatt catcaagttc
     3721 aaagaacaat tcaggtgtct tataaatact tagattgtgc cctaactctc gacgaatcaa
     3781 tcctgatgct ttattcaatc catcttgtgc ttttttctta tctgatgcta agtcagataa
     3841 aatgctgtaa tagatcgttg cttgctgtaa gtcccccgta actttcacat ctgtgatcgt
     3901 tacgttctcc acacgagggt cacggacctt tttgcgcaaa atatcattga cttcttttaa
     3961 gatttcttga ccgactcggc gatcacggta gttagccata atgattggcc tccttttatc
     4021 agtttttagt catttttgac ttcttccatg ataaatcctt caattacgtc atctactttg
     4081 atgtcattga atttttcaat catcgcacca cattcataac ccattttgac ttctttcacg
     4141 tcatctttga atcgtttcag acttgcaagt tggccttcat agattacgat tccgtcacgg
     4201 attacacgga caccgctatc tctacgaata tatccttctg ttacataggc acctgcaatc
     4261 gtaccaactt tagaaacttt gaaggtttca cgaacgatca tttgaccggt gattttttct
     4321 tcaaattcag ggtctagcat ccctttcatc gcagtttcga tttcttcgat ggctttatag
     4381 atgatacggt gaagacgaat atcgacttct tcagtatcag cttgcacttt tgcctgcggt
     4441 gtaggacgaa cgttgaaacc gatgataata gcgttgctgg ctgctgctaa ggttacatca
     4501 ctttcattga ttgctccgac agctgaatgg acgattttta cacggacacc ttccacatca
     4561 atcttcttca atgatgcagc cagtgcttct gctgaacctt gtacatctgc tttgatgatt
     4621 acgttaactt ctttcaattc gccttctttc aggctttcaa ataagttgtc caaagtcaca
     4681 cggcttgtgg cagcacggtt ttccaataat gcacgttttg cgcgttcttc acccgctgca
     4741 cgtgctgtct tttcgtcttc gaagactacg aaacggtcac ctgcttgagg tacatcattc
     4801 aagccagtga tttctactgg cgttgcaggt cctgcttctt tatcgcgacg gccaagatcg
     4861 tttgtcatta cacggacacg tccgtaagta ttaccgacaa cgattgggtc accaacgtga
     4921 agtgttcctt cttggacaag taaggttgac acaggacctt tacctttatc aagacgtgct
     4981 tcgatcactg ttccgattgc gcgttgtgtt ggatctgctt ttaaatcttc tacttctgcg
     5041 actaacagga tcatttccag caattcttcg atgttttggc cgaatttcgc tgagatctca
     5101 acaaaaatcg tttctccacc ccatgcttca ggaattaatt catactcact taattcttgc
     5161 ataacatgct gaggatttgc acctggttta tcaattttat ttacagcgac gatgattgga
     5221 acgcctgcag ctttggcatg gttgattgct tcaactgttt gtggcatgac gccatcatct
     5281 gcagcaacaa ctaagatcgt gatatctgtg atacttgctc cacgtgcacg catacttgta
     5341 aaggccgcat gtcctggtgt atccaagaaa gtgattggtt taccatcaat atcgatctgg
     5401 taggcaccga tatgctgtgt gattccgccg gcttcgccgc ttgttacacg tgaatggcgc
     5461 aatgtatcca ataaggttgt tttcccgtgg tctacgtgtc ccataatcgt tactactggc
     5521 ggacgtgaga ctaatttatc tggatcgact tcttctgcct cgaagaattt atcgatatca
     5581 gcaatgtcaa cttggacttt ttcttgtggt tccatgccat agtccgtcgc taacagttca
     5641 atcgtatctt tatccagtgc ttggttttga ttgaccatga caccaagcat aaataatttt
     5701 ttaatgattt ctgctggctc acgatgaatt tttttcgcaa tatctgctac gttcatacct
     5761 tcagtatact ctaatacatc tggcaattca cggaatttgc gtggtggtac agctggttta
     5821 gttgattgtt gctgtttgcc ttttttacct tttttattat aacgattacg gttattatta
     5881 ttaaatttgc cgcgattgtt attgtttggt ttgttgttat tgttagtacg gttttgattg
     5941 ccgccttggc tcgttccttg acgattttgg ctgctggtat ttcccgtatt attttggttt
     6001 gttgttggtt tttttccttg attcacttga ccgctccctt gaccgcgatc ttgataactg
     6061 cggttatttt tatttttttg ttgattgttc gttgtttctt ttttacgttg atcgttcggc
     6121 tgttgattgg ctggttttct gcttgcttgt tgtgcagctt gttgtttcgt gcttgggttt
     6181 ttgaacgctt gttgcaattt cttctcatca ctgctagtaa tcgcacccat atggtttttc
     6241 acatcgatgc caagttgttg tgctttttcc acgacgtctt tactagattt tttgagttct
     6301 tttgctaatt catagattct tttgttgccc atgcaatcac cttcctaacc ttcaattagc
     6361 tccttggtct tcttcgcaaa tccggcatcc attattccga ataccttgcg aggttttccg
     6421 atcatctgcg tgatttctgc ttcagaaaat agctcaaagc aaggaacttc ataatatgaa
     6481 ctcttatctt ttatcttttt tcttgtattc tcacttgcat cggttgccac aaacaccaat
     6541 tttgcttttt gtttgcggat ttcagcaatg gtcagttctt cacccgtgat tagttttccc
     6601 gcgcgcatcg ctagaccgat gagattcatg gctttttgcc gattaactgt attcattttc
     6661 caaataactc tttacgtgct ttttggtgag tgacatagtc aagaagttct tgataaaact
     6721 catctgttaa tgttgtttcg agtactcgat cgagaatttt tttatcccaa gcttgttgta
     6781 cttcttccgg ctcaagtgag acatacgctc ctcgtccagg catttttcca gttggatcga
     6841 tcgatacttc cccttctttt gaacgggtga ttcggaccat ctctttttta ggtttcattt
     6901 cacctgaaac cacggatttg cgtaaaggaa ttttcctttg tttcatctct ttgcctcctt
     6961 actaaagtta agcttctggt ttttcttcga ccgtttcatt gttgaatgca attgtttcat
     7021 attcatcgtc tgttaaatct gactgaacga ttgcttcatc atgcagttct tctgtttctt
     7081 tttgttgttc ttgattttca taaaattccg tcatgtcaga ttctgatttg atatcgattt
     7141 tatggttagt caatttagca gctaaacgtg cattttgtcc gcgtttaccg attgctagcg
     7201 ataattggta gtctggaacg acaactgtgc aggcctttgg atttgtctca tcgaagatca
     7261 catccatgac ttgtgcagga ttcaatgcat ttgcgatgaa tacagctgga tcttcattcc
     7321 attcgacgat atccatgttt tcgcctttta attcattgac gattgcttga actcgttgtc
     7381 cctttggccc gacacatgta ccgactgcat cgatatttgg atcatttgag cgaactgcca
     7441 cttttgaacg atcgccagct tcacgggcga tgctgacaat ttcaacgatt ccatcataga
     7501 cttctggtac ttcttgttca aataagcgac gcaataggtc tggatggctg cggctaacaa
     7561 atacttgcgg acctttagac gtattttcta ctcgggaaac ataaactttg attcgatcat
     7621 gtggttgata aaattcattt ggcatttgat cttgtttaga aagaactgcc tcaattttac
     7681 caagattgac ataaatataa cgtttatcct ggcgttcgac gattccttgc atgatgtctt
     7741 tttcataagc actgaattcg ttataaataa tcgttctttc tgcttcacgt acacgctgta
     7801 aaataacttg tttagccgtt tgcgcagcga tccgtccaaa atcttttggt gttacttcaa
     7861 aacggatcgt atcaccgatc tcatatgctg gattgattaa aagtgcatct ttcaatgaga
     7921 cttccaactg tgaatccatc acttcttccg ttacttcttt taccgcatag acatggatct
     7981 ttcctttttt ctgatcaaat tccacttcga cgttttgtgc ttggccatag tgacgtttgt
     8041 atgcagaaac caatgctgct tctaaagcat cgatcacgat ttcttttgag atcccttttt
     8101 cagcttctaa agcgtctaac gcatttaaca tttctttact catttttttc tgattcctct
     8161 ccgtgattaa aattggatag ctaagcgagc ttttgcaata ttttttcggt caaaagtcaa
     8221 ttctttttct cgtgttttta tgcgtatttt caatgtaagc tgttcagcat caaatgattg
     8281 aagaaatcct tcatattgtt tttcgccatc tactggttgg tataaggaga catggacata
     8341 atcaccaagt gccttttcat agtcgctttc ttttttcaat ggccgctctg ctcccgggga
     8401 ggatacctcc aggaaataag cttgaggaat cggatctgga tctgctgcat caagtttttc
     8461 actcagtttc tcactgacaa aggcgcattc ttcgatatcg ataccgcctt ctttgtcaat
     8521 aaatactcgt aagaaccagc tttttccttc ttttacaaat tcgacttcga caagttcaaa
     8581 attttgttca tctaaaatcg gtgtcaccat ttctgtgact gtttcaacga cgctactcaa
     8641 aagtttcgcc tccttaaaaa ttaagtgcgt aattccatta aaaagagtga gcgaaaaatc
     8701 cgctcactca cagttagtat cattacctat aagaactata ccatactctg cttaaaatta
     8761 caagcagaca ggaaaacaga tgcttatagc atatcgaata acgataattg attttcatct
     8821 ggtagatctt tcaatacgcc attgtcgttc atatattcga tcaatgtttt tgaaacttta
     8881 ccgcgggtgg caagatcttc tttggataaa aatggaccgt cttttcttgc ttcaacgatt
     8941 tgtttcgcca cgttggttcc caaacttggg acagctctaa atggtgcgat caacgtatca
     9001 ccttcaataa caaagttaac ggcatccgat ttatacaaat caatcattcc aaatttgaat
     9061 cctcgttcca gcatttcatt ggctaactcc agcactgtca gttgattctt ttcttttacg
     9121 gaagcatcta atcccttgtc cgtgatttct ttcattgctt gtttcacagc atcttttcct
     9181 ttgcacatgg agactagatc aaagtcgtct gcacgtacag aaaagtatgc acagtaatac
     9241 aaaataggga aataaacctt gaaataagcg acacgaagag ccatcaatac gtaagctgca
     9301 gcatgggctt tcggaaacat atatttgatt tttgaacaag aatcgatata ccagtcgggc
     9361 acattatttt ctttcatcgc cgatagatag gtttctcgca attcatcagg gatcttgttc
     9421 cattgtcctt tacgaacagt ttccatgatt ttaaatgcca tcccgctatc taatccagca
     9481 tggatcaaat aaaccataat atcatcacga cagccgatta cttccgccag tgtcgcatct
     9541 cctcgtcgaa tcaattcttc cgcatttcct aaccatacgt ctgtaccatg agatagtccg
     9601 gaaatttgca gtagttctgc aaaagttgta ggatgcgttt cttctaacat ccctcgaaca
     9661 aaacgagtgc cgaattctgg aatacctaat gtccctgttt tggaataaat ttgttcctga
     9721 cttactccaa gtacttcagg accggcaaat atccgcatca cttccgggtc atccgtaggg
     9781 atcgtttgtg ggtcgatccc tgataaatct tgaagcatcc gaataacagt tgggtcatca
     9841 tgccctagaa tatctaattt caatacatta tcgtgaatcg agtggaaatc aaaatgagtc
     9901 gttttccatt ctgaattttg atcgtctgct ggatattgga tcggcgtaaa atcatagaca
     9961 tccatataat cgggaataac gatgatcccc cctggatgct ggccggttgt ccgtttgact
    10021 ccagtagcgc cttttgctaa tctgtcgact tctgcacttc gaaactgcag attattgtcc
    10081 ctttcgtatc cttttacata gccatatgct gttttgtcgg cgactgtacc aatcgttccg
    10141 gcacggtaaa catagtcttc gccaaacaat actttcgtat agttatgggc ttctgcttga
    10201 taatcacctg agaagttcaa atcaatatcg ggcactttgt ctccatggaa tcctaaaaat
    10261 gtctcaaatg gaatatcatg accgtctttg tttaagcgtg caccacattt tggacattgt
    10321 ttttctggca tgtcaaatcc agaaccatac gtaccatctt caaaaaactc ggaatattgg
    10381 cactctggac aataataatg aggggctaat ggattaactt cagtgattcc tgtcatcgtc
    10441 gcaacaaagc tagagccaac agagccacgt gaacctacta gatagccatc ttcattactt
    10501 ttatgcacta gtttttgggc gatcagataa ataaccgaga agccattacc attaatagaa
    10561 ttcaactctt tttctaatcg tttttgaatg atctcaggta acggatctcc gtacatttgt
    10621 tttgcttttg tgtaactcaa ttctgatatt tcatcttctg aacctggaat cttcggtgta
    10681 taaagttcat ctttgactgg aatcacttct tcgcagatat ctgcgatttt attcgtattt
    10741 tctactacga tctctttcgc tgtttcttcc cctaaaaagt gaaatgcagt tagcatctca
    10801 tcggttgttc ggaaatggac atccggcaaa ctatgacgat tcaacggatt cgctccgccc
    10861 atagaattga tcaaaatttt tcggtaaatg gcatcttctt cgttcagata atgtacatta
    10921 cctgttgcaa caacgatttt atccaaactt tttccgattt ctaccaaatt ttggataatt
    10981 tcttccaaat catgttcatt ttttaccaat tcttgctcga tcaaaggcgc atagaccgct
    11041 tttggcataa cttcaatata atcatagaat tttgctcggt ttcttgcctc ttctacccct
    11101 ttttgcatca tggcttcgaa tatttctccc ttatcacaag ctgagccgat caatagattt
    11161 tcacgcatct ttttcaattg ggagcgtggt attcgtggga cgcgttcgaa gtattcgaca
    11221 ttcgacattg aaatcagttt gaacaaatct ttcagtcctg cctgattttt tgctaaaatc
    11281 gttacatgga aaggtcgagc ccttttgtaa gaatctcctt ctccgatatg ctcatttagc
    11341 tgatcatgat aaagcatgtt atgattgtcc attgcctctt tcacaaagat ccatgctaag
    11401 tgaccagttg cttcagcatc atagattgct cggtgatgct gttccaagct aacaccaaac
    11461 tttttagata acacacctaa tccgaaacgt ttgaattgag gatacagata tctagctaat
    11521 tccaacgtat caatgactgg attggccgct tcaggaatac cataccttgc gtagctagta
    11581 ttcaagaacc ccatatcaaa agccgcgttg tgggctacca aaattgtatc ttttgaaaat
    11641 tcaaggaaca tccgcaatac ttcttcttcc gactttgacc ctcgaaccat ttcatccgtg
    11701 ataccagtca gatctaccgt tgttcgtgac aatggatgtc ctggatcgat aaattcatca
    11761 aagctttcga tcacatttcc tttatacatt ttgactgcgg ctagctcgat gatcgtgtca
    11821 tatactgcag acaatcctgt cgtttccacg tcaaatacga cgtatgtggc ttcactcaat
    11881 gcttcatgtg catcattata agcaattggg acaccatcat ctacgacatt cgcttccacg
    11941 ccgtataaga ttttcacacc tgcttttttt ccagcactat gtgcttccgg aaaggcttgc
    12001 gcaccaccat ggtctgtaat ggcgatagct cggtgtcccc attttcctgc ttgtgccaca
    12061 agatcagaga tgctgtttgt tgcatccatc gtactcatat tgctatgaac atgaagctct
    12121 actcgttttt ccccttcagg agcataatct ttgcgaggtg tatgtttgac ttctatgata
    12181 tcttgcgcat tcatgaccaa atcacggaca aacgtatctt cttgaatact tcctcgtacc
    12241 tttaaccagc taccgacact gatcgcatca aatacttgtt catctttttc gccgttcgag
    12301 aattttttga cgataaatga tgaagtataa tctgtgattt tcaaagtcaa gatcttgcgt
    12361 tttgaacgta gttctctgac ttctttgtcg aatacgtacc cctcaagtgt gactcttcgt
    12421 tcttcttcaa cgatactgat cattggtgtc gttggttcat cggctggaat atttcgtcct
    12481 agctggatgg gtccttcaag agctggcgat tgctcttttc tttccttttt cttttgttcg
    12541 tggacaacta gactttcagc tgcttgcttc ataaaggctt ctgcctgttc ttgtttgcgt
    12601 tcttcaaaaa gtttcaatac acgttccgct tgctgctcat ccacttctgg ttcgatacga
    12661 aattttggga atccgtaact gacgaacaac tcttctacta atggcaagta ttgttgcttc
    12721 aaataagaaa tagctcctgt actgtcgatt ggcaagatga ctttacgatc ttttatcaca
    12781 ggtacctgcg ttttcaatac ttgctgtgct agtggtgtat cgcattgctg attttccaaa
    12841 gcttgggccc agtaatcttg caagagctgt tcatcgaatg tctggtcatc tgctaagata
    12901 ttcagtttga cttgtgcaat atctttaaaa gcgatagtca gatgctgagt caatgaccga
    12961 tatagcataa taggcaaaat tttggaaaaa tgtagcgtaa attcccataa acgcgattta
    13021 cgatggacca ctacactatc catttcacca gcagtaagca gtggatgatt tttttctgct
    13081 tcctctagtt ggatttgttc caacagtttt tcaaataaat ctcgtttttc tgacaaagat
    13141 atacgccctt tctgatgaga aacaatgcaa ttgatcgcat cttatcctct tctttttcag
    13201 taccttttag tatactcgtt ttttctgagt tttacattaa aaaattggaa ctgcgattca
    13261 aatcgcagtt ccaattttaa aagcgaatca tcagattgtc aaaaacagct gattcagcta
    13321 tttttatttt ctgtgtttta acgcttatta ttcgccttgg ttcaagagga ttggtaaagt
    13381 attcgccaat tcttctttac gtacttcaac catttcgcca gttttcttga ttttcacttc
    13441 tacgattcca tccactgctt ttttaccgac agtgattctg attggacagc caatcaaatc
    13501 agaatccgcg aatttcactc ctgcgcgttc atttctgtca tcaaccagta cttcatagcc
    13561 agcttttgtc atcgcttctt ctagttcatt cgtcaggctt gtttggtatt cgtcttttag
    13621 attcatttgg acgatatgca gatcaaatgg cgcaattcct tttggccaat tgattccttg
    13681 ttcatctgaa tgttgttcta cgattgctga taataaacga cttacaccga taccataaca
    13741 gcccataatc acaaattttt ctcgtccatt ttcgtctagt acagttgctc ccatagattc
    13801 actgtaacga gtgccgattt tgaatatatg tccgatttcg attcctcgag taaaggcaag
    13861 aacaccgttg ttgtctggtg aaggatctcc ttcttgtaca aaacgaagat cttcataaga
    13921 aagcacagtg aaatcgcgtt ttggattcac atttttatag tggaatcctg tttcgttcgc
    13981 tccagtaatg gcattcgcta aatcttggac ataacggtct gcatatactt ttacgtcatc
    14041 tgaaacaccg actggtccga cagaaccaaa ctcagcacct agatattttt gtgcttcttc
    14101 ttctgttgct tcttctaaaa agtcagcaga taaaaagttt ttcagtttta cgtcattgac
    14161 ttcatggtct ccacgtacca aaacaaggac aggttgttca tccgcaatga acaatacaga
    14221 cttgatgatt ttttgaggtt caacagaaaa gaaatctgct acttcgtcaa ttgattttac
    14281 gttaggtgta gctactttct ccaattcaag ctgtgtttca tgcgattttt ttgatacata
    14341 gtagcttgta gccatctcca agtttgcagc ataatcactt tcagtcgaat aacagatcgt
    14401 atcctccccg atttctgaga tcgccatgaa ttcctttgag tctttcccgc ccattgcgcc
    14461 gccgtctccg atgatcgagc gaaattctaa gccacaacgc ttgaatactt cggtataggc
    14521 tttttcgtaa tctttatatg tctcgtccaa actttcttcc gttgcatgga aagaatatgc
    14581 atctttcatg ataaactctc ttccgcgaag caaaccgaag cgaggtcgtt tttcgtcacg
    14641 atatttaggt tggatttgat aaagatttaa aggtaatttc ttatatgaat tgatttcatt
    14701 acgcatcaaa tcagtaaaag tttcttcgtg agttggtcca aggatcattt ttcgatcatt
    14761 gcggtctttg aaacgataca agtttggtcc atatgtttcg taacgcccgg attcttccca
    14821 aagttctgct ggcaagatgg caggcatcag catttctacc gctccgattt tttcaaattc
    14881 ttcccgcata atgcgtttca gtttttccaa tacacgattt gctaatggca gatatgaata
    14941 aatacccgct gatacttggc ggatatatcc agctcttagc aagatttgat ggcttaatac
    15001 ttctgcatca tttggaactt cgcgtaatgt tggaatcaac atttttgatt gtctcattct
    15061 atatactcct ttatttcctc aaaatagtta tcgtttgtta ttatacccta tttgtcaact
    15121 ttttctagaa aaagaatcgt tgaatatcgt tccatgtaac tagcaccatc aacaacatta
    15181 aaaatccaaa gccagctaat gtcaatattc cttctttttc ttgacttaat ggtttcccgc
    15241 gaatcccttc aaaaatattc aagactaatt tcccgccatc taaggcagga atcggaagca
    15301 gattgacgat tccgagattc attgaaagaa gcgccatcaa actaataaca gttgttacac
    15361 cttgatttgc tgcttctgaa gacaattggt acatcatgac aggtccgcct aatttatcta
    15421 aactgaaacc tgtaaatagt gatcctaagg ctttgaaaat ttccaatgaa ccgctgaaag
    15481 cttgacgcgt ccctccaatg atcttatcca taaaacctgt attcattggg gcttttatac
    15541 caagttggcc aactttttcg ccatttgact ctacagattt cggtgtcacg tcgacagatt
    15601 gcacctgacc ttctcgttcg atcttaaagt ctaatgtttt acctggattt ttcgtaatga
    15661 ctgttgtcag atcattccat gaatggattt cttttccgtc cacactgact acctcatcat
    15721 tttcttttaa gcctgcttct gccgcagctc cattaggcat gatctctcct acgcgattag
    15781 tgttcgtcac ttgaacacca ccttgcataa atgccagaat gatgaataac acgattgcca
    15841 aaataaagtt attcatgggt ccagcaaagt tggtcagcat acgctgccac aatttagccg
    15901 attggaactg tacgtctttt ggcgcaattc tgatttctgt tccgtcgttt tcaataatcg
    15961 tcgcatcgtg atcaacaggg taggttacta cttctgtttc gtctccattc acataaccag
    16021 tgatagtcag ctcatcttct aaatcataac ggctcaattc catcgggata gcatttgtca
    16081 attggatttt cttacttaaa tttatcttct ctacgatgcc gtcagaattt agtaaaagag
    16141 aaagaggcat tcctggtgcc atttctgttt catcatcccc attgcctgcc attcgaacat
    16201 aaccgccaat aggtaatagg cgaagcgtat atgttgtccc gtcttttgct tgatgcccat
    16261 aaattttcgg acccataccg atagcaaatt cgcggacgag gattcctgat cgtttcgcaa
    16321 agaagaaatg accaaactca tgaacaatca ctaatatacc aaaaacgatg ataaatgtca
    16381 gaatcgtttt catagctttt ttctccttta cttgtttttt gtgtttttta taaaacaacg
    16441 ataaaacact ataattttac catagtatac caaagacacc aatagtatca atataatgaa
    16501 aaataaaaac ctgcgtcttt tatgaaattc acaaagaaac gcaggtatac ttactaaata
    16561 atgccaaata aatgcatgat aggaaataca aacagcaaac tgtcgaaacg gtctaagatt
    16621 ccaccatggc ctggtaagat atttcccgag tctttcacat cataatgacg tttgattgca
    16681 gattcgacca aatcaccaaa ttgtccgact gctgaaaaaa taatcgtcat cagaagcatg
    16741 acaaacatac catgattgaa tagttctttt gctgggtata gcaataaata aagaaatgcg
    16801 actacaacag cactaccgat accaccgagc gcaccttcaa tcgttttgtt gggcgaaata
    16861 tcaggactta acttatgctg accataacgt cgaccaacca tatatgctcc gatatcagtt
    16921 gcccaaacaa taaatagacc aaatatcaaa acagccaagc tttctgttcg tgcattgaca
    16981 aaattctgga aacctattcc cacatacaag ctgacaagca caggaaaacc tgcttcatca
    17041 atcgtatacg tatttttaga aataactgat gttcctaaaa gaatcataac tataagataa
    17101 aataacataa atgtacttac attctctgga agaaagaaaa accatctttc ttttggcaaa
    17161 acaagtaaca ctgcaccaac ggcggataaa actccttcaa aacttaagag tgtcaatcct
    17221 ttcatccgga acagctcgta aactcctact accgctaaga ttgctgctgt aagctcaacc
    17281 gctatgcctc cgatccatat aatcggaata aacaagacta gcgccacagc tgctgtgatc
    17341 acacgttgtt tcatgactga tcccctttct catcttctgt tttttttaca ccgccgaatc
    17401 gacggtctcg attttgataa gaggcgattg cttcttcaag atgcgcacca tcaaaatcag
    17461 gccaaagcgc ttttgtaaaa tacagctcac tatatgcgat ctgccataac agaaaattac
    17521 taattcgctc ttctccactt gtacggatca tcagctcagg atcttgtaac tctttaggca
    17581 agaaacctgt cattaaatgc ttagcaatca attcatcatc gatttcttct gcgtgtattt
    17641 ctccttttgc tacttcttcg gcaatttctt ggacagcagt cacgatctct gctctacttc
    17701 cataattcaa agcaaaattc aataccattc cagtattgtt ttttgtttgc tcaattgcac
    17761 gtcttacagc atcttgcgta tgttctggca atacgttttc ataacccatg acatgaactt
    17821 tgacattttc tttaatcaat tctggtacga acgtatcaaa gaagtcgaca ggtagttgca
    17881 tgagaaaact tacttcatct tttggtcgct tccagttttc agtcgaaaag gcataaagcg
    17941 tcaacacttt cactccaagc cttgatgctt tttttgtgac tttctttact gtttccatgc
    18001 cttctttatg gccagcaact cgtggcaaac gacggttttg cgcccaacga ccattgccgt
    18061 ccatgatgat ggcaatgtgc tgaggaattg gtccatcggt attaaattga aattcgcttt
    18121 tttcttgaat atatttgttt ttttgcggaa aaaaacgtaa cattcttttt cctcctatac
    18181 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
    18241 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
    18301 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
    18361 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt taatgctgtt
    18421 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
    18481 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
    18541 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
    18601 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
    18661 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
    18721 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
    18781 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
    18841 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
    18901 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
    18961 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
    19021 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
    19081 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
    19141 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgctcgtaat
    19201 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
    19261 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
    19321 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
    19381 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
    19441 cccatttgtg ctccgatttg tccgcgccag atatttccgc cacccacaac gattgccatt
    19501 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
    19561 ttgattccaa atcctgcatc cccagctaaa gcttctccac tcaattttaa gactacacgc
    19621 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
    19681 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
    19741 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
    19801 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
    19861 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
    19921 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
    19981 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
    20041 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
    20101 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
    20161 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
    20221 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
    20281 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
    20341 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
    20401 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
    20461 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
    20521 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
    20581 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
    20641 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
    20701 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
    20761 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
    20821 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
    20881 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
    20941 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
    21001 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
    21061 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
    21121 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
    21181 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
    21241 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
    21301 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
    21361 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
    21421 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
    21481 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
    21541 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
    21601 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
    21661 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
    21721 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
    21781 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
    21841 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
    21901 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
    21961 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
    22021 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
    22081 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
    22141 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
    22201 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
    22261 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
    22321 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
    22381 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattaaat
    22441 aaacctttac actctttaca aaaatacatc attttttatt caattttttc aacaccttga
    22501 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
    22561 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact cctggaccat
    22621 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
    22681 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
    22741 aaggaatata cacaacggtc ttaatgcaag gtagcggctc ctttggggta gaatcggtat
    22801 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatggac
    22861 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
    22921 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccctctatct
    22981 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
    23041 ttgctgctgt cacacaagaa aaagatcttt tgtttatagt ggacgcgatg tcaagctttg
    23101 gtgggatacc gattcgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
    23161 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactagaaa
    23221 agcgaaaaaa tgtgagtcga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
    23281 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
    23341 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
    23401 ccaataatca tgtactacga caaaaaatga cagagatggg gttccagcct ttcattcaag
    23461 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
    23521 atcattttta tcagtattta aagcaaaacg gatttgtgat ctatcccggg aaaatcagcg
    23581 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
    23641 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
    23701 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
    23761 tttctagaaa aaggaatcca actgactgac aaagaaatcc gcgaaccgat gggaaaatta
    23821 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
    23881 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
    23941 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
    24001 gaacggttga gaaacgctgg gatcaatatt ggttccacga ctggatttac tgctcctatg
    24061 atgacaatcg tagcagaaac cgcaaaaaaa gaagggtatt caccagactt catggcaact
    24121 tctgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
    24181 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
    24241 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
    24301 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaaagatcg tttaaaacag
    24361 tctgttgctc ttagattcca acaagctggc gctgatattt gtgtaaacaa tttaaaagag
    24421 ttaacagaat acattttgta cgctgacaag aatccagaat gacttttata caaaatcaga
    24481 ggtcgtgaaa aagctgtact gcatcaagta ataagaacag ccaaacaaaa atagctcctc
    24541 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    24601 gaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttattta caaacaaaca
    24661 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
    24721 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
    24781 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
    24841 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
    24901 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
    24961 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
    25021 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
    25081 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
    25141 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
    25201 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
    25261 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
    25321 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
    25381 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggcgca tatcgtcaga
    25441 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
    25501 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
    25561 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
    25621 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
    25681 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
    25741 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
    25801 acggaaccat tgccataatt gaatagcctc ttcatcgcct tcttccagac gtttgaacca
    25861 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacata
    25921 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
    25981 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
    26041 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
    26101 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
    26161 tccttgattc ccgatattgc tatccccata atgttctttt tctttcacta ctgtttgcaa
    26221 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
    26281 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
    26341 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
    26401 tgattttgga ttttctagta attgttcgac ttgttccagt gttaaatcat ctttgacagc
    26461 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgct
    26521 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
    26581 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
    26641 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
    26701 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
    26761 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
    26821 tagtcgtttc tgataaaaaa acaatccaga tggcctaaag gagaagaaat accacctgga
    26881 tagctataaa tcatttcgta aatacgaaag acttaacaaa ttaatcagct acgataatcg
    26941 ttccttcttc agaagctaaa agattctcga tattttctaa agaagtgatg attgccttag
    27001 cgtttggttt tgcttcaaca aatgcaatag ctgcctctac tttcggaagc atgcttcctg
    27061 gagcaaattg cttttcatca atgtattgtt tcatttctga aacagtaaca gtttctaatt
    27121 tcttttgatc tggtttttgg taattgacat acacattgtc tactcctgtc aatacgatca
    27181 gcaaatccgc atcgatgatt tcggctaatt tttctgaagc aaaatcttta tcgattacgg
    27241 cttctcgtcc ttctaatcca gtagctgttt ctacaacagg aatcccaccg ccaccaacag
    27301 agactgtgat aactccttga tcgaccagcg tttcgatgac acgtgcttct ttaatcgaga
    27361 tgggtttagg ggaagcgact acttttctcc atccacgtcc agcatcttct ttgaatgtca
    27421 cagtggagtc agcattcatt tgttctttgg cttcttcttc cgtgtagaat ggaccgactg
    27481 gtttacttgg atttttgaat gaaggatcat tttcatccac aataacttga gtgactaaag
    27541 aaaccacatc tttatcgatc cctttttcct tcagtacttc gcccatggca ttttgtaacc
    27601 agtaaccgat agatccttct gtcattgcaa cacatgtatc taaaggcatt gccggtgttt
    27661 tctcagaatc agcagcttgc tgctgaatca ataggttacc gacttgcgga ccatttccgt
    27721 gggagatgat caattcatct ccttgttcga taaatttcac taaatacttt gctgtttcca
    27781 ttaacgcttc ttgttgggct tttgcacttg catccgtaga caagattgca ttacccccta
    27841 aagcgacaac tacttttcgt ttggccattt cattcgtcct ttcttcagtt ctaccgaatt
    27901 ttctagataa caaaacgagc cagcgttttg tgattttccc tcaatgctga ctcgtttttt
    27961 cgtgagatga atgtggttat gcttaaacgc gagggataaa caagttacct aatgttgctg
    28021 ccatgatcgc tttgattgag tgcatacgat tttctgcttg gtcaaattga cgagcatatt
    28081 tgctgcggaa gacttcatct gttacttcca tttcagtgat gccaaagcgt tctttcattt
    28141 gttcaccgta aacagtgttt gtatcatgga aagctggcaa gcagtgcaag aagatcaaac
    28201 gatctgtgtt gtgtgttttt tctaccattt ccatattgat ttggtaaggt ttcaacaatt
    28261 tgatacgttc ttcgaattta tcttcttcac ccattgatac ccatacatca gaataaagga
    28321 cgtctgctcc gtctacacct ttatcaacat cgtcggtaat catcaattgt gcaccggatt
    28381 tttcagcaaa gccttctgcc attttgacga tttcttcttc aggttgtaat tcttttggtg
    28441 caacgatacg catattcgct cctaaaattg cacctgtgac taataaagag ttagccatat
    28501 tgtttcgacc atctccgcag taagcaacag tgataccttc tactgttcca aagttttctt
    28561 ggatagttaa aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc aagccattcc
    28621 aaactggaac accagagaat tctgctagtt cctctaccat tttttgactg aatccacgga
    28681 attcgattcc atcaaacata cgacctagta ctttagctgt atcttctgtg gattcttttt
    28741 tacctaattg gatatcattt gcacctagat attcaggatg tgcacctaga tcgatcgctg
    28801 cagtagtaaa tgcagaacga gtacgtgtag atgttttttc aaataaaagc gcaatatttt
    28861 ttccttctag atagtgatgt gggataccac gttttttcaa gtctttcaag tgttctgaaa
    28921 aatcgatcaa atattgtaat tcttcttttg taaagtcttt ttctgctaat aagcttcttc
    28981 cttggaatac tgattctttc atattaaaca ttctccttta gattgttttt atttaagatt
    29041 aaagaggaaa tcccgacggc agctgacagt aagaccgccg tcaggaaaca aattacttca
    29101 agtcttcacg gacaagtggt tggctcatgc agcgtggacc accacggcct cgtgacaatt
    29161 cacttgaatt gatttctaac actttgatgc catagctacg aagcaattcg ttcgatacat
    29221 aatttcggtt ataagtgact acaacacctg gagcgattgc caatgtgttt gatccatcat
    29281 tccattgttc tcgaggtgca acgatctcat ctccattacc agttgggatc agtacaagat
    29341 catctaatcc aagagcagct ttcatcgttt cgtgtaagtt atctgaatga gtaatcttga
    29401 tatcatcacc atctggttcg attgtaaaga catccatctt accgtcttta ctttgaattg
    29461 ctggatggat agtgaattta tcgtgatcta ccattgtaaa tacggtatcc aaatgcatca
    29521 ttgcacggtt gtttggaatt ttgattgcta acactttttc aaatcctgaa ttttttgcga
    29581 ataggtggcg tgctaatttt tctaaagctt tcgcattagt tcgttgagaa atcccaacag
    29641 ctactacttt atcgcttaag atcaattcgt ctccgccttc gatatgatct ggattttcac
    29701 gatccaacca tacttcgatt cctttatttg caaatcttgg atgatgtttc aaaatatatt
    29761 ctgtaaacat cgattcacga cgacgtgctt caaaagtcat cttattgact gtcatcccat
    29821 ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg tccatgtaaa
    29881 atggatagtc atcgtctgct gacagatcgt atagactgct agaacgtact tcaatatctt
    29941 ttgtacgaac cccagccatg attttatcta ccatttcttg cgtttccatt gatagaagga
    30001 actcatgaag accctctctt acagcattcg atgcaatatg agattcatcc aacattttat
    30061 tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat ttttccaagt
    30121 aaagagtctc gacgccttca ttttgcaatg tctttgcaaa gttgtcatgt tcttcttgag
    30181 caatcggaag atatggaata tcatcgaaca acagacgatc cataatatct ggtgtcaaat
    30241 tttccacttc ttgtccaggc cttttcagta aaactgtctt taatttccct atttcagaga
    30301 aaacgtgaat aggtttatcc atgttgtgtt gcctccttct ttttgattac atgtatagga
    30361 tatcctcctt ttgtaatcac tttcataatt ttgtctcatt caaaatgaga cattcctcac
    30421 attaatattc gataattaaa taattaaacg tttttctatt tttaagcgta atttattca
//
LOCUS       DAZKSU010000030        30422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000030 DAZKSU010000000
VERSION     DAZKSU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.030"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..187)
                     /locus_tag="ACG8Z3_001376"
     CDS             complement(59..187)
                     /locus_tag="ACG8Z3_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0823696.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8Z3_001377"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="ACG8Z3_001377"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0823697.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="ACG8Z3_001378"
     CDS             complement(1674..2729)
                     /locus_tag="ACG8Z3_001378"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0823698.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8Z3_001379"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="ACG8Z3_001379"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0823699.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8Z3_001380"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="ACG8Z3_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0823700.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8Z3_001381"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="ACG8Z3_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0823701.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8Z3_001382"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="ACG8Z3_001382"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0823702.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8Z3_001383"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="ACG8Z3_001383"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0823703.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="ACG8Z3_001384"
     CDS             10491..12050
                     /locus_tag="ACG8Z3_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0823704.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="ACG8Z3_001385"
     CDS             complement(12161..14092)
                     /locus_tag="ACG8Z3_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0823705.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8Z3_001386"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="ACG8Z3_001386"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0823706.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="ACG8Z3_001387"
     CDS             complement(15021..15773)
                     /locus_tag="ACG8Z3_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0823707.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="ACG8Z3_001388"
     CDS             complement(15932..16141)
                     /locus_tag="ACG8Z3_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823708.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="ACG8Z3_001389"
     CDS             complement(16152..17480)
                     /locus_tag="ACG8Z3_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823709.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="ACG8Z3_001390"
     CDS             17616..17771
                     /locus_tag="ACG8Z3_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0823710.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8Z3_001391"
     CDS             complement(17845..18432)
                     /gene="yihA"
                     /locus_tag="ACG8Z3_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0823711.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8Z3_001392"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="ACG8Z3_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0823712.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8Z3_001393"
     CDS             complement(20012..21301)
                     /gene="tig"
                     /locus_tag="ACG8Z3_001393"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0823713.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="ACG8Z3_001394"
     CDS             complement(21455..22387)
                     /locus_tag="ACG8Z3_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823714.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="ACG8Z3_001395"
     CDS             complement(22544..23203)
                     /locus_tag="ACG8Z3_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0823715.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8Z3_001396"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="ACG8Z3_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0823716.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGASGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="ACG8Z3_001397"
     CDS             complement(24244..24534)
                     /locus_tag="ACG8Z3_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823717.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8Z3_001398"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="ACG8Z3_001398"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0823718.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8Z3_001399"
     CDS             complement(26821..28176)
                     /gene="glmM"
                     /locus_tag="ACG8Z3_001399"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0823719.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="ACG8Z3_001400"
     CDS             complement(28200..29369)
                     /locus_tag="ACG8Z3_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0823720.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8Z3_001401"
     CDS             complement(29366..30253)
                     /gene="cdaA"
                     /locus_tag="ACG8Z3_001401"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0823721.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
BASE COUNT         8187 a         6588 c         5440 g        10207 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacattagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagcc cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 tt
//
LOCUS       DAZKSU010000031        29109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000031 DAZKSU010000000
VERSION     DAZKSU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.031"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1030)
                     /locus_tag="ACG8Z3_001402"
     CDS             complement(<1..1030)
                     /locus_tag="ACG8Z3_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0823722.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="ACG8Z3_001403"
     CDS             complement(1112..1801)
                     /locus_tag="ACG8Z3_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0823723.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACG8Z3_001404"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="ACG8Z3_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0823724.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACG8Z3_001405"
     CDS             complement(2328..2831)
                     /gene="rplJ"
                     /locus_tag="ACG8Z3_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0823725.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACG8Z3_001406"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="ACG8Z3_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0823726.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACG8Z3_001407"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="ACG8Z3_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0823727.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACG8Z3_001408"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="ACG8Z3_001408"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0823728.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACG8Z3_001409"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="ACG8Z3_001409"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0823729.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7543)
                     /locus_tag="ACG8Z3_001410"
                     /pseudo
     CDS             complement(6443..7543)
                     /locus_tag="ACG8Z3_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7635..8522)
                     /locus_tag="ACG8Z3_001411"
     CDS             complement(7635..8522)
                     /locus_tag="ACG8Z3_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0823730.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACG8Z3_001412"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="ACG8Z3_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0823731.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACG8Z3_001413"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="ACG8Z3_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0823732.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACG8Z3_001414"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="ACG8Z3_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0823733.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /gene="cbpA"
                     /locus_tag="ACG8Z3_001415"
     CDS             9753..10397
                     /gene="cbpA"
                     /locus_tag="ACG8Z3_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0823734.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="ACG8Z3_001416"
     CDS             complement(10453..10956)
                     /locus_tag="ACG8Z3_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0823735.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="ACG8Z3_001417"
     CDS             11553..12518
                     /locus_tag="ACG8Z3_001417"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0823736.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="ACG8Z3_001418"
     CDS             complement(12617..13789)
                     /locus_tag="ACG8Z3_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0823737.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="ACG8Z3_001419"
     CDS             complement(13776..14921)
                     /locus_tag="ACG8Z3_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0823738.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="ACG8Z3_001420"
     CDS             complement(14935..15867)
                     /locus_tag="ACG8Z3_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823739.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="ACG8Z3_001421"
     CDS             15984..17063
                     /locus_tag="ACG8Z3_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0823740.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="ACG8Z3_001422"
     CDS             17060..17689
                     /locus_tag="ACG8Z3_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0823741.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="ACG8Z3_001423"
     CDS             complement(17799..18026)
                     /locus_tag="ACG8Z3_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0823742.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="ACG8Z3_001424"
     CDS             complement(18129..20717)
                     /locus_tag="ACG8Z3_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0823743.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="ACG8Z3_001425"
     CDS             complement(20737..21396)
                     /locus_tag="ACG8Z3_001425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0823744.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACG8Z3_001426"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="ACG8Z3_001426"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0823745.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="ACG8Z3_001427"
     CDS             complement(22169..23035)
                     /locus_tag="ACG8Z3_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0823746.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="ACG8Z3_001428"
     CDS             23144..23785
                     /locus_tag="ACG8Z3_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0823747.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACG8Z3_001429"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="ACG8Z3_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0823748.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="ACG8Z3_001430"
     CDS             complement(25226..26125)
                     /locus_tag="ACG8Z3_001430"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0823749.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="ACG8Z3_001431"
     CDS             complement(26127..26924)
                     /locus_tag="ACG8Z3_001431"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0823750.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="ACG8Z3_001432"
     CDS             complement(26902..27606)
                     /locus_tag="ACG8Z3_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0823751.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="ACG8Z3_001433"
     CDS             27737..28312
                     /locus_tag="ACG8Z3_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFQ0823752.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="ACG8Z3_001434"
     CDS             28430..29107
                     /locus_tag="ACG8Z3_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0823753.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8565 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaaaa
//
LOCUS       DAZKSU010000032        28032 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000032 DAZKSU010000000
VERSION     DAZKSU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.032"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..293
                     /locus_tag="ACG8Z3_001435"
     CDS             123..293
                     /locus_tag="ACG8Z3_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823754.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            513..1220
                     /locus_tag="ACG8Z3_001436"
     CDS             513..1220
                     /locus_tag="ACG8Z3_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0823755.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1279..1923)
                     /locus_tag="ACG8Z3_001437"
     CDS             complement(1279..1923)
                     /locus_tag="ACG8Z3_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0823756.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2049..2336
                     /locus_tag="ACG8Z3_001438"
     CDS             2049..2336
                     /locus_tag="ACG8Z3_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823757.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2444..2764
                     /locus_tag="ACG8Z3_001439"
     CDS             2444..2764
                     /locus_tag="ACG8Z3_001439"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0823758.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2845..4158
                     /locus_tag="ACG8Z3_001440"
     CDS             2845..4158
                     /locus_tag="ACG8Z3_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0823759.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            4155..4874
                     /locus_tag="ACG8Z3_001441"
     CDS             4155..4874
                     /locus_tag="ACG8Z3_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0823760.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            5002..5553
                     /gene="raiA"
                     /locus_tag="ACG8Z3_001442"
     CDS             5002..5553
                     /gene="raiA"
                     /locus_tag="ACG8Z3_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0823761.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5775..8309
                     /gene="secA"
                     /locus_tag="ACG8Z3_001443"
     CDS             5775..8309
                     /gene="secA"
                     /locus_tag="ACG8Z3_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0823762.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8435..9536
                     /gene="prfB"
                     /locus_tag="ACG8Z3_001444"
     CDS             join(8435..8506,8508..9536)
                     /gene="prfB"
                     /locus_tag="ACG8Z3_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0823763.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9641..10327
                     /gene="ftsE"
                     /locus_tag="ACG8Z3_001445"
     CDS             9641..10327
                     /gene="ftsE"
                     /locus_tag="ACG8Z3_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0823764.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10320..11204
                     /gene="ftsX"
                     /locus_tag="ACG8Z3_001446"
     CDS             10320..11204
                     /gene="ftsX"
                     /locus_tag="ACG8Z3_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0823765.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            11356..12804
                     /gene="cls"
                     /locus_tag="ACG8Z3_001447"
     CDS             11356..12804
                     /gene="cls"
                     /locus_tag="ACG8Z3_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0823766.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13141..13998
                     /locus_tag="ACG8Z3_001448"
     CDS             13141..13998
                     /locus_tag="ACG8Z3_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0823767.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14113..15033
                     /gene="pstC"
                     /locus_tag="ACG8Z3_001449"
     CDS             14113..15033
                     /gene="pstC"
                     /locus_tag="ACG8Z3_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0823768.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15033..15917
                     /gene="pstA"
                     /locus_tag="ACG8Z3_001450"
     CDS             15033..15917
                     /gene="pstA"
                     /locus_tag="ACG8Z3_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0823769.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVSEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15932..16738
                     /gene="pstB"
                     /locus_tag="ACG8Z3_001451"
     CDS             15932..16738
                     /gene="pstB"
                     /locus_tag="ACG8Z3_001451"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0823770.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16754..17512
                     /gene="pstB"
                     /locus_tag="ACG8Z3_001452"
     CDS             16754..17512
                     /gene="pstB"
                     /locus_tag="ACG8Z3_001452"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0823771.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKNKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17525..18202
                     /gene="phoU"
                     /locus_tag="ACG8Z3_001453"
     CDS             17525..18202
                     /gene="phoU"
                     /locus_tag="ACG8Z3_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0823772.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18778..19470
                     /locus_tag="ACG8Z3_001454"
     CDS             18778..19470
                     /locus_tag="ACG8Z3_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0823773.1"
                     /translation="MLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            19467..20234
                     /locus_tag="ACG8Z3_001455"
     CDS             19467..20234
                     /locus_tag="ACG8Z3_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0823774.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20334..21902
                     /gene="liaX"
                     /locus_tag="ACG8Z3_001456"
     CDS             20334..21902
                     /gene="liaX"
                     /locus_tag="ACG8Z3_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0823775.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22199..22519
                     /locus_tag="ACG8Z3_001457"
     CDS             22199..22519
                     /locus_tag="ACG8Z3_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0823776.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            22519..22869
                     /locus_tag="ACG8Z3_001458"
     CDS             22519..22869
                     /locus_tag="ACG8Z3_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0823777.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23036..23974
                     /gene="hprK"
                     /locus_tag="ACG8Z3_001459"
     CDS             23036..23974
                     /gene="hprK"
                     /locus_tag="ACG8Z3_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0823778.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23989..24819
                     /gene="lgt"
                     /locus_tag="ACG8Z3_001460"
     CDS             23989..24819
                     /gene="lgt"
                     /locus_tag="ACG8Z3_001460"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0823779.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24863..25888
                     /locus_tag="ACG8Z3_001461"
     CDS             24863..25888
                     /locus_tag="ACG8Z3_001461"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0823780.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25903..26841
                     /gene="galU"
                     /locus_tag="ACG8Z3_001462"
     CDS             25903..26841
                     /gene="galU"
                     /locus_tag="ACG8Z3_001462"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0823781.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26966..27406
                     /locus_tag="ACG8Z3_001463"
     CDS             26966..27406
                     /locus_tag="ACG8Z3_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0823782.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27409..28011
                     /locus_tag="ACG8Z3_001464"
     CDS             27409..28011
                     /locus_tag="ACG8Z3_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0823783.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         9278 a         4817 c         6065 g         7872 t
ORIGIN      
        1 taccacaatc gtaagctctc gttagttcga gagctctttt ttttgctttg ctaccttgtt
       61 ccttgaagat tctgctgatt taagatagag ttaagttaac tacaagaatt ggagggatcc
      121 acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa gttatatgat ttggtcaaat
      181 cagaaaaata caatattgaa gtgaatattc cgaaaaaagg cggaaaagcg gtccgggtaa
      241 aatcaaaacg cccaacgaat catacaaaaa aatggatggc taaaaaccgc taataataac
      301 aaaagatcca taagtcggca ttttcatcga ttttagggtc ttttgttatt ttctaggttg
      361 gacatatcta aaaaattgtt tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc
      421 ctctgttgat ggatagaaac ctttatcttt ttcagttgtg aatcaggtat ataccagtta
      481 taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa tcaattacct gtatatatac
      541 agattcatga ccaattaaaa aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc
      601 ttccttcaga aagagaatta gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag
      661 caattcaaaa cttggcagat gaaggaatct tagaaagaaa gatcggttcg ggtacatatg
      721 ttgcacgaga aaaagtacaa gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg
      781 aatcacaaaa tcgagtaccg tctagtcgga caatctctta tttcttgact tcgccaagct
      841 caagtgaaat ggaaaaacta aacttgagta aagatgacac gatcttgcga atggaacgta
      901 ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc cagtgtgcca caaaagctga
      961 tcgatggcta cagcaaagca gagattactg aatcgttgta tcgcacgcta gaagaaaaag
     1021 gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa
     1081 ttgctgacta tttagaggta aaacgaggag atgcagttct tcgtttgcgc cagatttctt
     1141 attttgaaga cggtacgccg tttgagtatg tgcgtacgca atacgtagga aatcgttttg
     1201 agttttattt ggaaaaataa aaaacaggtg atccctgatt ccgtattaga aataaaagag
     1261 aatcagaatc acctgttttt atatatgatc agttaccagt tgctcgtgat aagaaacagg
     1321 accctcttca aattggactt gtccgttcag cagctcaatg atggattctt taaagtggtc
     1381 agcacttttt tcatctacag cacagatgac gctaacttga tcagtatatt ggatttcttt
     1441 tataaaaaaa tgttggagtt caagataatt ctgtaatttt cctagttgcg gatacgtgat
     1501 catcacagag atctcctgtt gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg
     1561 agagaccgca tgagtgtatg ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa
     1621 atatcgagtg actactgcag ctacattgat cagttgattt tttttgagga cttccaacat
     1681 cggtatgccc gccgttccac ttggttctcc atcatcgctg cttcgctgga tttcattttt
     1741 ttctccaatg atgaaggcgc tgcagttatg gttcgctttc caatgttctt tttttatttc
     1801 ttgaatgaaa ctttttgctt cgtcctctga ttctattcgt ctaagagaac agataaagcg
     1861 tgattttttg atctcgatct cactttgtcc attttctttt attgtacgat agcgctcgat
     1921 catcttgtca cctctttttt cattttcttt attcagtata aataaagttc taggttgttt
     1981 caacactgta acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg
     2041 atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca
     2101 atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta
     2161 acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc
     2221 ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg
     2281 acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa
     2341 accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt aacagacgcg
     2401 aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta aaaaaacaat
     2461 catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc
     2521 tgctgaagca aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa
     2581 caatcttgca aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa
     2641 agctgatttt gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc
     2701 tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc tcataggtga
     2761 ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc
     2821 gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga
     2881 gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca
     2941 gtggaacgtt tgagcaactg gattctttgt catcgctgtg gaatgagaac gtccgtcatt
     3001 gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt agggcgatgc
     3061 gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc
     3121 tttttttgga caggacagct tactgaaaaa caaacggaaa tctcaaagag aatactttgc
     3181 cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg
     3241 ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg
     3301 cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa
     3361 atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt
     3421 acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc
     3481 gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac agcgattaaa
     3541 ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact aaaggagatc
     3601 aagcagacgt ttgacatcca caaacttgct ttacgctttc atcggcgttt gcttcctgaa
     3661 cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa gaagataaaa
     3721 tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt attttgtcca
     3781 agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc gagatatgag
     3841 atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa aatgcgagaa
     3901 gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac atttgaaaga
     3961 atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc tttggtacaa
     4021 atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata ttttttctat
     4081 gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat gaatgaagaa
     4141 gggaagaaga tactgtgagg tgcagttgct gcagtaaaga tatcgtccaa aatttaacat
     4201 tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca gtttgtcgcg
     4261 cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca atccatttca
     4321 atctagagag tacgaaaaag aaagtaaatt ctatccccgt ttactgcagt gactgtcaag
     4381 aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt gtgtacgatg
     4441 aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga ctagcaggca
     4501 cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc tattgtccaa
     4561 tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc gagatgttga
     4621 aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg ctggctcaag
     4681 caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt gcagtcacct
     4741 tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca acaggacaga
     4801 ctcttttcca cgcagcagat tgtttgcaga tcgcacagcc gaaaatcatc cgaactattt
     4861 ctttggcgag atgaattaga tgtggaaatt tgatagcgat ttaatttgca aataacctag
     4921 agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg
     4981 tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta
     5041 actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt
     5101 gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa
     5161 gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat
     5221 ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa
     5281 acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcaaatgc tgctgcttta
     5341 ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc
     5401 ctttcattga aaccaatgga tagtgaagaa gcagtcttgc aaatgaacat gctgggacat
     5461 aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa
     5521 gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata acaacgaaaa
     5581 cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc ttttagatga
     5641 aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca attatctata
     5701 actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag aaggaaaagg
     5761 agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat aagaaagaac
     5821 taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa atggcagcat
     5881 taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac caaaaaggag
     5941 aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca gcaaaacgtg
     6001 tattaggttt atatccatac cacgtacaat tgatgggtgg aatcgtgctt cacgatggaa
     6061 atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg ccagtatatc
     6121 taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat ttagcaaccc
     6181 gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg gggttgaata
     6241 tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc acttatagta
     6301 cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac cgccatcaaa
     6361 tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc ttgattgatg
     6421 aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct ttatatacaa
     6481 gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc gatgtacaat
     6541 caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca ttcggtttgg
     6601 ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa gcattgagag
     6661 ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa gtactgattg
     6721 ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga cttcaccaag
     6781 caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg gcgacgatca
     6841 ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt acagctaaaa
     6901 cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt ccgacaaatc
     6961 gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa agcaagttcc
     7021 acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg ttagtcggaa
     7081 ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa cgtattcctc
     7141 atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg aatgctggtc
     7201 aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat atcaagttag
     7261 gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga catgaatctc
     7321 gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct ggtatgtcac
     7381 agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa cgaatcaaag
     7441 ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc aagatgctga
     7501 cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac acacgtaaaa
     7561 acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat aaacagcgac
     7621 aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg gtcaaacgga
     7681 caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg aatttggaaa
     7741 gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact cttgcagata
     7801 tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca caagaagtat
     7861 ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt gaaaaagtcg
     7921 tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgacgcaatg gatcaattac
     7981 gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa tatcaaacag
     8041 aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc actcgaatct
     8101 ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa ggaacagctt
     8161 ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa aaacagccaa
     8221 tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg aaaaaatata
     8281 agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg agacaaattc
     8341 tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt agactagata
     8401 taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa ttcgaaatct
     8461 cctgacagaa atgcaggaaa aggtcacaag tttcaggagg tctctttgac ttagatcagc
     8521 tggaagaaga catcgcggaa gcagaagcaa aaatggctga acctggtttt tgggatgaca
     8581 gcgaagccgc acaaaaagtg atcagtgaaa ataacgctaa taaagaaacc tatgaccaat
     8641 tccattctct tgccgaagaa ctagaagaaa tcgaattgat ggtggagatg ctgcaagaag
     8701 aacctgatgc agcaatgcaa gaagaagcag aaacacgtat caatgcatta gatgaaaaaa
     8761 tgaagacgta tgaattatct atgctgttag acggacctta tgatcgaaac aatgcgatcg
     8821 tcgaattgca tccaggggct ggcggaacgg agtcccaaga ctggggcagc atgcttttac
     8881 gtatgtatac acgttgggca gagcagcatg gctatcaagt agaaacacta gattatcaag
     8941 caggtgatga agcagggatc aagagtgtga ccctgctgat taaaggacat aacgcttttg
     9001 gttatctaaa atcagaaaaa ggtgttcatc gccttgtacg gatttcgcct tttgactcag
     9061 cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt gatgaaacga
     9121 tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca agtggtgcag
     9181 gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg cctacaggaa
     9241 cagtagtagc tagtcaggca caacgctcac aattgaaaaa ccgtgagcaa gcgatgggga
     9301 tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa gcagctgcac
     9361 ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat gttttccatc
     9421 cctattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc caagcagtta
     9481 tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta caataagaac
     9541 ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac attgccatgc
     9601 tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa tgaaggatgt
     9661 aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcgattg tcatcgaaca
     9721 aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat tcatcaaatt
     9781 gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc atgatctaat
     9841 agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg tgttccaaga
     9901 ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta tgcaagttat
     9961 cggcagaaaa cctcgagata tcaaaaaacg agtaatggaa gtgctggacc ttgttggttt
    10021 gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc aacgtgtctc
    10081 tattgctcgg gcaatcgtga atacgcctaa agtacttatc gcggatgaac cgacaggaaa
    10141 tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca atgcccaagg
    10201 aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc ggcaccgagt
    10261 aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat atggatacga
    10321 tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa cgtaacggct
    10381 ggatgacagt tgcttcagtc agtgcggtaa cgatcacgtt gactttagtc ggtgtattca
    10441 tggcagttat catgaatgca actaaattag ctcaggatat agaaggaaac gttgatgtat
    10501 ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa aaagaattga
    10561 atgacttaga tcatgttaaa aaagttgaat tttcaagtaa agaacaggaa ttgaaaaaaa
    10621 tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac ccgttatatg
    10681 atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca gaagaagcag
    10741 cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat cggattttcc
    10801 aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg ctgttcgtag
    10861 cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag cgtgaaattc
    10921 aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt ttccttgaag
    10981 gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca tttggttatg
    11041 catggttttt ccaattgatc aatccatcat tattacgttc ccattattca ttgattcatc
    11101 cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg atcattggtt
    11161 cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac aatataaata
    11221 aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta ggagaataga
    11281 aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta agttggtaga
    11341 ggaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct tttgattatg
    11401 aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc tcaaacatgg
    11461 gcatggctgc tcgttttgat gttcattcca gttgtaggat ttattttata tattttttta
    11521 ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa aatcggtaag
    11581 aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc tcatccgcct
    11641 actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga aagtacgctc
    11701 tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt cgatgcgctg
    11761 attgaagata tcaagcaagc agaagaccat atccacttcc agtattatat ttatcgcagt
    11821 gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg aggggtaaaa
    11881 gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt ttttcagaat
    11941 ttgaaaaaag caggcggaga aatcgcattt ttctttcctc tatttgttcc gtatattaat
    12001 cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa aatcggatat
    12061 acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt tggttattgg
    12121 cgagacaatc atttgcgtat ttacggagaa gcggtgtata tcttgcaaaa ccgttttttg
    12181 atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt ttttccgtcc
    12241 attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga taccgaacat
    12301 gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga gatattgatc
    12361 caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt ggctcttctt
    12421 tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct cgtttactgg
    12481 gcaacgtatt ctttcgctgc agagctgatc gagtatggag caaggatcga aacatatgag
    12541 aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc agttggctca
    12601 gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact gatttatgat
    12661 gaacgaatgg cttcacgtgt aagaaaagca ttttttgaag actcgaaaat ttctacgcat
    12721 ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga aggattagca
    12781 agattgattt cgccgctcct ttaaaacaca aaaaggacat gagaaccatc aacagatgga
    12841 gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat ggagaaagcg
    12901 tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa gcaagaaata
    12961 agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata gaaactttta
    13021 atgagatagt tttttttaca attatgagga tttattttgt tatttaatga ttttgattcg
    13081 ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag gagatttcta
    13141 atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt atcaggctgt
    13201 tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc tttacagcct
    13261 ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt catcaatgta
    13321 caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt cgacatcgga
    13381 aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt agtcgatcat
    13441 cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt aaaagacatc
    13501 agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag tgaagtcggt
    13561 ggaaaagatc agccagtagt tatattgaac cgtgcttcag gaagcggaac ccgtagcacg
    13621 tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca agattcaagc
    13681 ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt tgctttttct
    13741 tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac agatgaaaat
    13801 gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag agatcatcca
    13861 aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca agataacatc
    13921 gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga ttggcaagga
    13981 aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac ttttacatgt
    14041 tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa cctgattaag
    14101 ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa
    14161 atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt gttggttgta
    14221 ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac tgataagatc
    14281 aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg agcaaatggg
    14341 aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac ctttttatct
    14401 gctattgttg ccacaccatt tgccattggt gcagcagtat tcatgacgga gatctctcct
    14461 aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat tccttctgtc
    14521 gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat ttttggcggg
    14581 acagggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt gccgactgtc
    14641 acaacgatga ctgttgatgc attaaaggca gtaccacggc actatcggga agcttcatta
    14701 gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc
    14761 gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc
    14821 caaatggtca tcggtaatgc agcattgatg ccaacaggac ttactactcc tgcttctaca
    14881 ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt tgaaaataat
    14941 gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa tatcgtgatc
    15001 cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca gataaaattg
    15061 ctacaggtgt tttatatgta gtttcgggga tcatcgtatt gattttagca gctttactac
    15121 tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc gaaccttcca
    15181 aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt tatttgcttt
    15241 tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat ctttcagaat
    15301 atgctggaaa aaactggctg acagatgtga ttcgcacgtc gattgaaatc ttgagctcat
    15361 tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa ttccagtatg
    15421 gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct cttttgacta
    15481 gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca ggattggctt
    15541 taggtttgtc acgctgggaa acagtgataa aagtggtggt ttcagaagcg acaccaggga
    15601 ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct gcattgatct
    15661 atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca ttaagtgttt
    15721 caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt tggaagatca
    15781 atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct gccgtgttga
    15841 tcattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa
    15901 aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa
    15961 tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc tacatgtctg
    16021 gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac
    16081 tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat
    16141 gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat
    16201 taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg tttttcaacg
    16261 tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga aacagcacgg
    16321 cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac aagctgcctt
    16381 gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg gcgggcaaca
    16441 gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc tgctagacga
    16501 gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac ttgtaaactt
    16561 gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcgg ctcgtatcag
    16621 cgactatact gcctttttct atctaggaaa agtaatggaa tatgacaaaa ctaacaagat
    16681 atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt ttggttaaga
    16741 cggaggcctc aacatgacaa aagaaattat ttcatctaaa gatcttcatc tgtattatgg
    16801 aaagaaagaa gcactaaaag ggatcgatct aagtttcaat caaggggaaa tcactgcgat
    16861 gatcggtcct tctggttgcg ggaaatccac ttatctgcgt tctttgaacc ggatgaatga
    16921 cctgattccg aatgtaacga tcaccgggag tgttttatat aaagacaaag acatctatgg
    16981 tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc agcagccaaa
    17041 cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa aaggggaaaa
    17101 aaataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt ctgtttggga
    17161 tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc agcagcagcg
    17221 tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg atgaaccaac
    17281 aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag aattaagaga
    17341 gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta tttcagataa
    17401 gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga agatattcat
    17461 gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat aaggaggaaa
    17521 cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc agttctacga
    17581 aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca tcaaacacga
    17641 taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca tggaaacacg
    17701 cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa ctgatttgcg
    17761 tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg atcatgcggt
    17821 gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa
    17881 agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc tagttgctta
    17941 tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa gagtcaatga
    18001 ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc ctgagacagt
    18061 catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg gagactatgt
    18121 gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg agttaaatac
    18181 gaaccacaat gagattttct aaactaaagc ggtgattctg ccgtttgttt aggaaaagag
    18241 gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga acagtcaaac
    18301 aaaaatagct cctcatattt ttcatttggc tggacttatt accgcagatg caagcttttg
    18361 aacactgttt attcgggaaa gggtttgtga caaaatatgt cacaaaccct tttcatcatt
    18421 tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt ttttatttgt
    18481 gtgcttactt catgggttat tcagcatcaa gctgctttat atgaagtacc tgtcatcgag
    18541 atcgaaaaag tacgaataaa agaggatcat caattgatta caggaagact gatgaacacg
    18601 aatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca aatagaaaca
    18661 gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga atggcagcta
    18721 gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat tggcgccatg
    18781 ttgcttattg gcggaaaaca tggtctattt gctttaggaa gtctcatcat caatatcgtt
    18841 ttacttttac ttttgttgct catctttact aaaatagaag ggatttctct attaaatata
    18901 tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga tggatggaat
    18961 agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc attttttatt
    19021 tgtcttttga caatgaatct ttggaaagac caaggactgc gctatgagga actgaattat
    19081 ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac agctggagga
    19141 acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa gcgacaacca
    19201 gaaataacag cgaaacagtt atggctttcg ggaaggcaag tgggtcgaga tgtgattggc
    19261 tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc gcttctttta
    19321 tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc attggaattt
    19381 ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc ggtcctcttt
    19441 tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt gggattttag
    19501 caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta ggcttattca
    19561 tcaatttagg gatctttttc ttgttgttat tcggttatca tcagaagtgg ccgatactgg
    19621 cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt atcaacggtt
    19681 ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt tgtttcctac
    19741 tattgcttcc tatcactgat tttttagcaa tccaaggttt tacaacgata gaactagaag
    19801 aattatctgg cttagataag actttagcga ttgattttag attacttacg agatcgttgc
    19861 ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct agtgggacat
    19921 tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat tctagtttcg
    19981 ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca ttcatgggaa
    20041 gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag caagtgatca
    20101 atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg tccgcactac
    20161 ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag aaagaacaac
    20221 tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa tcagtgcata
    20281 atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag
    20341 aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag
    20401 atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa gaagcagaca
    20461 aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat aaacttgaaa
    20521 acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga caagatcaag
    20581 aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact tcggcgcgtc
    20641 tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag aaacaggaag
    20701 cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa gaattagcac
    20761 agcgtcagac gttagaagca gaaatcaaag agttagaagc tagtggcgat acactgttag
    20821 aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa tggtctgaga
    20881 aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat caagcaacag
    20941 atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta ggaaaattct
    21001 tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa tggaaagatg
    21061 tcagcttgcg tgttcctggg attgcgacaa cgaaatttga gcatgaattt tactatgaag
    21121 ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt
    21181 gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa atgggtgctg
    21241 aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat catatctcct
    21301 tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct aaacgagtat
    21361 acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca ttggaagcta
    21421 aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta actgcaacga
    21481 tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac atcttagatt
    21541 ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa aacctttctg
    21601 tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg aaaaaagtag
    21661 aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc ggtttggaag
    21721 gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac gaagtagttc
    21781 gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca gatggtgaaa
    21841 tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat acagataaat
    21901 aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt gtaaaatagc
    21961 ttcccctatt ttacaaaatt gtgccttatt gccgataggc tatctcttga acaccgttta
    22021 ccaagagatt atgaacttgc caattatcgc cgagtcacaa ggaataattt caaaaaaagc
    22081 tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcaaaaa gacaccgatt
    22141 aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga ggtgagacat
    22201 gatgaaaaaa aagttgacaa aatccaataa aaatgttgtt ttgacaggga ctttagcagg
    22261 aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg tattcctgag
    22321 cttagttgct tttggaagtc caatcatttt atacatttta ttagctttga ttatcccatc
    22381 tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa aaaacaatta
    22441 tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa tcgatgatga
    22501 cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac gttaacattc
    22561 atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg gacggccgtt
    22621 cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt gacgatactg
    22681 tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa tgcagcgatt
    22741 cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc attcggtgca
    22801 gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga acataacagt
    22861 agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta gaatcaggaa
    22921 tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg tagtactttt
    22981 ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag catgaatgtc
    23041 agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg tgacaggcga
    23101 cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag gactagagtt
    23161 gactggttat tttaattatt attcccataa tcgtttacag ctatttggca gtaaagaaat
    23221 cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa gattatgtag
    23281 tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag aaatgatcca
    23341 ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt ctcgcttatt
    23401 aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg tccacggtgt
    23461 tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga taggaaaaag
    23521 tgaaacagcg ttggagttga tcaaacgcgg tcatcgattg atcgcagacg atcgagtaga
    23581 tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc tggaacatct
    23641 gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg cgagtgcggt
    23701 acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa aagataagaa
    23761 atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtag gcattccaca
    23821 aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag tagccgcgat
    23881 gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag aaagattaag
    23941 tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat gttagcacaa
    24001 atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc attgatcatc
    24061 gtatcaggta tcattatcgc catgtggctt agcagcagag aggctgttcg ggtaggatta
    24121 aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat tattggcgct
    24181 cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat ccaaatattc
    24241 aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc gatcacgctg
    24301 tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat cgctgcacca
    24361 agtgtattat tagcacaagc aatcggtcgt tggggaaatt tcatgaacca cgaagcatat
    24421 ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt catcattgat
    24481 aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc actttggagt
    24541 ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt gaaaaaaggt
    24601 gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat tgaaggatta
    24661 cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt atctgctgtg
    24721 ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa tttaccggat
    24781 tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg ttaaaaatga
    24841 aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac caggctcttg
    24901 gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga tttggggaca
    24961 tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt atttaccaga
    25021 tcattgtcta cccgattcca tccaagcatt caccaatatg gaagatgcag taaaagacgc
    25081 agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa aagaactagt
    25141 gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag aacaggggac
    25201 acataaaagg atctctgata tcttgttaga ggaaatccca gtagaaaaaa gaaaagctgt
    25261 tgttgttctt tctggaccaa gccatgcaga agaagtagcg aaacatgaca tcactacgat
    25321 cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt tcatgaataa
    25381 ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg cagctttgaa
    25441 aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg ataatgcaaa
    25501 ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag caatgggtgc
    25561 caacccgctg acctttatcg gattaagcgg agtaggcgat ctagtcgtta cttgcacaag
    25621 tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc ccctagaaga
    25681 ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag cagcgatgga
    25741 actagcccaa ataatggaaa tcgaaatgcc catcactcaa acaatctatg aagtgctgta
    25801 tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg ggaaaaaaga
    25861 aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt caaaaaagca
    25921 gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc tatggcaaag
    25981 gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga agcgttgaaa
    26041 tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat cgaagatcac
    26101 tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac cgaattattg
    26161 aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc tcatccaaag
    26221 ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc atttgtcgtt
    26281 atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt aatcgatgat
    26341 tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga tgaaacttca
    26401 aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa tgtcaaaaac
    26461 tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat tggacgttac
    26521 ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc cggaaatgaa
    26581 atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt tgcccgtaaa
    26641 ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac aagtattgaa
    26701 tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct gtctttaggt
    26761 aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat aacagaaatc
    26821 aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta attgaataaa
    26881 aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc cttatagaaa
    26941 agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat tgatcgcagc
    27001 aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag tttctaaaac
    27061 tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa tcgtcacaaa
    27121 agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta atgaactgct
    27181 cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag ttgctgattt
    27241 aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga aagtcggcgg
    27301 aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga cagcgatgaa
    27361 attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat ggctaaaaaa
    27421 ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc cgcgttattg
    27481 ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc ggatgatttg
    27541 atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga gttaacaggt
    27601 attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc aggtagtgtc
    27661 aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga tatcttgtta
    27721 aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc agaagatatg
    27781 aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga tatcatgatt
    27841 gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat gtcagaagca
    27901 aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga tgcgaaagaa
    27961 gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta aaatcacaaa
    28021 tttatagaat aa
//
LOCUS       DAZKSU010000033        27698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000033 DAZKSU010000000
VERSION     DAZKSU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.033"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG8Z3_001465"
     CDS             complement(287..649)
                     /locus_tag="ACG8Z3_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823784.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG8Z3_001466"
     CDS             complement(723..1103)
                     /locus_tag="ACG8Z3_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0823785.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG8Z3_001467"
     CDS             1185..2360
                     /locus_tag="ACG8Z3_001467"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0823786.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG8Z3_001468"
     CDS             complement(2437..3021)
                     /locus_tag="ACG8Z3_001468"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0823787.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG8Z3_001469"
     CDS             3222..4220
                     /locus_tag="ACG8Z3_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0823788.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG8Z3_001470"
     CDS             4332..4841
                     /locus_tag="ACG8Z3_001470"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0823789.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPSILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ACG8Z3_001471"
     CDS             complement(4871..5530)
                     /locus_tag="ACG8Z3_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0823790.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ACG8Z3_001472"
     CDS             complement(5532..6296)
                     /locus_tag="ACG8Z3_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0823791.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ACG8Z3_001473"
     CDS             complement(6304..6981)
                     /locus_tag="ACG8Z3_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0823792.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ACG8Z3_001474"
     CDS             complement(7018..8403)
                     /locus_tag="ACG8Z3_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0823793.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ACG8Z3_001475"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8Z3_001475"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG8Z3_001476"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ACG8Z3_001476"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0823794.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ACG8Z3_001477"
     CDS             9342..10199
                     /locus_tag="ACG8Z3_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823795.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ACG8Z3_001478"
     CDS             complement(10280..10615)
                     /locus_tag="ACG8Z3_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823796.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ACG8Z3_001479"
     CDS             complement(10682..12052)
                     /locus_tag="ACG8Z3_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0823797.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ACG8Z3_001480"
     CDS             complement(12130..12666)
                     /locus_tag="ACG8Z3_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0823798.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="ACG8Z3_001481"
     CDS             12975..14456
                     /locus_tag="ACG8Z3_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823799.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="ACG8Z3_001482"
     CDS             complement(14559..15266)
                     /locus_tag="ACG8Z3_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0823800.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="ACG8Z3_001483"
     CDS             complement(15345..15683)
                     /locus_tag="ACG8Z3_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0823801.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="ACG8Z3_001484"
     CDS             complement(15794..16756)
                     /locus_tag="ACG8Z3_001484"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0823802.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKINVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="ACG8Z3_001485"
     CDS             complement(16771..18102)
                     /locus_tag="ACG8Z3_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0823803.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="ACG8Z3_001486"
     CDS             complement(18110..18790)
                     /locus_tag="ACG8Z3_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0823804.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="ACG8Z3_001487"
     CDS             complement(19009..19488)
                     /locus_tag="ACG8Z3_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0823805.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19615..21294)
                     /locus_tag="ACG8Z3_001488"
     CDS             complement(19615..21294)
                     /locus_tag="ACG8Z3_001488"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0823806.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21842..23344
                     /locus_tag="ACG8Z3_001489"
     CDS             21842..23344
                     /locus_tag="ACG8Z3_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0823807.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23357..24019
                     /locus_tag="ACG8Z3_001490"
     CDS             23357..24019
                     /locus_tag="ACG8Z3_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823808.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24120..24323)
                     /locus_tag="ACG8Z3_001491"
     CDS             complement(24120..24323)
                     /locus_tag="ACG8Z3_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0823809.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="ACG8Z3_001492"
     CDS             complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="ACG8Z3_001492"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0823810.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="ACG8Z3_001493"
     CDS             complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="ACG8Z3_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0823811.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8242 a         5774 c         4676 g         9006 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct agtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt tatctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt
    19681 ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc
    19741 aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt
    19801 catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga
    19861 aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga
    19921 caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat
    19981 cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag
    20041 cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa
    20101 atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt
    20161 catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt
    20221 ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac
    20281 gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat
    20341 cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat
    20401 tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt
    20461 aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat
    20521 tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa
    20581 tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca
    20641 atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg
    20701 attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc
    20761 caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc
    20821 cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt
    20881 atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc
    20941 taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc
    21001 taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg
    21061 aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat
    21121 gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc
    21181 aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat
    21241 gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt
    21301 cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat
    21361 gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag
    21421 gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt tggatatact
    21481 tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca
    21541 gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa
    21601 caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc
    21661 atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac
    21721 tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt
    21781 taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa
    21841 tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt
    21901 attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag
    21961 cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag
    22021 tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac
    22081 tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga
    22141 aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat
    22201 ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac
    22261 tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc
    22321 agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac
    22381 agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt
    22441 cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac
    22501 gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag
    22561 cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta
    22621 cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc
    22681 tgctatttac actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa
    22741 gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt
    22801 ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc
    22861 cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt
    22921 aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga
    22981 agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc
    23041 gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca
    23101 agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg
    23161 tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat
    23221 caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt
    23281 ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa
    23341 ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca
    23401 agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt
    23461 aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc
    23521 aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta
    23581 tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc
    23641 tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca
    23701 agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt
    23761 gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga
    23821 acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga
    23881 tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac
    23941 tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt
    24001 tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct
    24061 gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt
    24121 tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct
    24181 ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg
    24241 tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta
    24301 gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct
    24361 tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag
    24421 ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt
    24481 ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga
    24541 tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac
    24601 gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca
    24661 cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat
    24721 gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat
    24781 ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat
    24841 ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt
    24901 tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa
    24961 attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt
    25021 ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac
    25081 gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg
    25141 atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag
    25201 aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc
    25261 cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt
    25321 ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga
    25381 cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac
    25441 agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct acttccactc
    25501 cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta
    25561 catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac
    25621 atttttcctc tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat
    25681 aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg
    25741 tttggattac ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa
    25801 tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg
    25861 cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg
    25921 gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg
    25981 atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg
    26041 aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa
    26101 tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg
    26161 aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta
    26221 cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga
    26281 ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa
    26341 agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc
    26401 agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat
    26461 tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg
    26521 aactttctgg ttttttacgg attttttctg catatatcat catataacca gcggctaatg
    26581 cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag
    26641 caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt
    26701 gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa
    26761 atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac
    26821 cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac
    26881 tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac
    26941 ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata
    27001 ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata
    27061 ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt
    27121 aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc
    27181 cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg
    27241 gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat
    27301 aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt
    27361 attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa
    27421 agatgactac accaaaaata atgacatacg tatggggtgt cgataattta gtgaagtcgc
    27481 gtttcggttt tcctgaagaa tttgttttct tcaaatttat ttcttccttt cttttttcag
    27541 taattttgat gaataaaaaa tggataatca aatgaattat acagtaaaaa tgacatttcc
    27601 acatgaataa tttattagaa aatcactttt ttctattttg tttttttctc ctatgtatga
    27661 ttattttttt ctctttccat ttatattaag tattttat
//
LOCUS       DAZKSU010000034        27673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000034 DAZKSU010000000
VERSION     DAZKSU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.034"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..1586)
                     /locus_tag="ACG8Z3_001494"
     CDS             complement(195..1586)
                     /locus_tag="ACG8Z3_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0823812.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1799..2185)
                     /locus_tag="ACG8Z3_001495"
     CDS             complement(1799..2185)
                     /locus_tag="ACG8Z3_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0823813.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2241..2864)
                     /locus_tag="ACG8Z3_001496"
     CDS             complement(2241..2864)
                     /locus_tag="ACG8Z3_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0823814.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2928..3596)
                     /locus_tag="ACG8Z3_001497"
     CDS             complement(2928..3596)
                     /locus_tag="ACG8Z3_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0823815.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDREDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3608..4048)
                     /locus_tag="ACG8Z3_001498"
     CDS             complement(3608..4048)
                     /locus_tag="ACG8Z3_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823816.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4107..4778)
                     /locus_tag="ACG8Z3_001499"
     CDS             complement(4107..4778)
                     /locus_tag="ACG8Z3_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0823817.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(4963..5361)
                     /locus_tag="ACG8Z3_001500"
     CDS             complement(4963..5361)
                     /locus_tag="ACG8Z3_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0823818.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5358..6131)
                     /locus_tag="ACG8Z3_001501"
     CDS             complement(5358..6131)
                     /locus_tag="ACG8Z3_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0823819.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6128..6811)
                     /locus_tag="ACG8Z3_001502"
     CDS             complement(6128..6811)
                     /locus_tag="ACG8Z3_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0823820.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6978..7865)
                     /locus_tag="ACG8Z3_001503"
     CDS             complement(6978..7865)
                     /locus_tag="ACG8Z3_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0823821.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7862..8731)
                     /locus_tag="ACG8Z3_001504"
     CDS             complement(7862..8731)
                     /locus_tag="ACG8Z3_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0823822.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8868..10154)
                     /locus_tag="ACG8Z3_001505"
     CDS             complement(8868..10154)
                     /locus_tag="ACG8Z3_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0823823.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10427..11005
                     /locus_tag="ACG8Z3_001506"
     CDS             10427..11005
                     /locus_tag="ACG8Z3_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0823824.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11005..11934
                     /locus_tag="ACG8Z3_001507"
     CDS             11005..11934
                     /locus_tag="ACG8Z3_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823825.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11931..13667
                     /locus_tag="ACG8Z3_001508"
     CDS             11931..13667
                     /locus_tag="ACG8Z3_001508"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0823826.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13730..14566)
                     /gene="pheA"
                     /locus_tag="ACG8Z3_001509"
     CDS             complement(13730..14566)
                     /gene="pheA"
                     /locus_tag="ACG8Z3_001509"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0823827.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14563..15072)
                     /locus_tag="ACG8Z3_001510"
     CDS             complement(14563..15072)
                     /locus_tag="ACG8Z3_001510"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0823828.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15065..16360)
                     /gene="aroA"
                     /locus_tag="ACG8Z3_001511"
     CDS             complement(15065..16360)
                     /gene="aroA"
                     /locus_tag="ACG8Z3_001511"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0823829.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16373..17464)
                     /locus_tag="ACG8Z3_001512"
     CDS             complement(16373..17464)
                     /locus_tag="ACG8Z3_001512"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0823830.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17481..18647)
                     /gene="aroC"
                     /locus_tag="ACG8Z3_001513"
     CDS             complement(17481..18647)
                     /gene="aroC"
                     /locus_tag="ACG8Z3_001513"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0823831.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18649..19713)
                     /gene="aroB"
                     /locus_tag="ACG8Z3_001514"
     CDS             complement(18649..19713)
                     /gene="aroB"
                     /locus_tag="ACG8Z3_001514"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0823832.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19746..20768)
                     /gene="aroF"
                     /locus_tag="ACG8Z3_001515"
     CDS             complement(19746..20768)
                     /gene="aroF"
                     /locus_tag="ACG8Z3_001515"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0823833.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21427..22401)
                     /locus_tag="ACG8Z3_001516"
     CDS             complement(21427..22401)
                     /locus_tag="ACG8Z3_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0823834.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22382..24265)
                     /locus_tag="ACG8Z3_001517"
     CDS             complement(22382..24265)
                     /locus_tag="ACG8Z3_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0823835.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24283..26508)
                     /locus_tag="ACG8Z3_001518"
     CDS             complement(24283..26508)
                     /locus_tag="ACG8Z3_001518"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0823836.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26623..27657
                     /locus_tag="ACG8Z3_001519"
     CDS             26623..27657
                     /locus_tag="ACG8Z3_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0823837.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT         7949 a         5999 c         4914 g         8811 t
ORIGIN      
        1 tatgaggaac tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca
       61 acctcttatt cacggtgttt cagaagtagc ttctcggaaa taagccgaaa tttcacaaaa
      121 atttgagaaa ctatttttag gaaacttctt cttatttctt aaagctgacc acttctatca
      181 caacctcttt ttgactatgc attcacatcg atcactttag aatcatcatt gatgacatcg
      241 cgatctaaat ctttgatgcg tgcacttgtc acgataccag ctaccatgct accgctgacg
      301 tttactgctg tacgtgccat atcgattaat ggttcaacag agataactaa cccaacgatt
      361 gctactggca agttcattgc ccctaaaacg atcagtgaag caaatgttgc gcccccaccg
      421 actccagcta caccaaaaga actgattgtt acaattgcca caagcatcaa aataaattcc
      481 aaactaaaga cattcactcc agctgctggt gcgacgatcg ttgccaacat agctggataa
      541 acgccagcac agccgttttg tccaatagac aaaccaaaac ttgctgagaa attagctgtt
      601 gcatcatcta ctcctaaagc ttttgtctga gtctcgatat tcaatggcaa agctccagca
      661 cttgaacgtg aagtaaacgc aaagctcaat accggaaagg cttttttgaa atagttgatt
      721 ggattgactt ttgcaccaat caaaatcagt gagtggacaa tcaaaacagc aagcaatgct
      781 acgtaagaag caacgacaaa tttacctaaa ttgacgatag ctgcaaaatc acttgttgcc
      841 aaaacgtttg tcatcaaagc aaatactcca taaggtgtta aacgcaaaac aagcgtcacg
      901 atccgcataa tgattttata cagactgtcg actaaatctg aaaagaatgc tgcttctttt
      961 ggcgcttttc ttttcacgcc tagataagcg attccgacaa atgccgcgaa aataacgact
     1021 ccaatcgtac ttgttgcacg actacctgct aaatcagcaa aaacgttttt agggataaag
     1081 gataaaatct gctgtggaat cgataagttt tcaactgttt cctgacgtgt tgccagttca
     1141 gagattcgtg ctgtttctgc agctccttgt gtgaatttag ctccttggag tccgaacagc
     1201 attgttgaac caattccgat caatgctgca atagctgttg ttccaagtaa agaagtcaat
     1261 acagtcgcac taattttccc tagcttttca gaaactttca tttttgtgaa tgcaccaatg
     1321 atcgaaataa acactaaagg cataactagc atttgcaaga atgatacata tccttgacct
     1381 acaatcccga tccattccat tgattgagta acgactttgt cgtctacacc aaagatcaat
     1441 tgaagcagac cgccaaagac gataccaact cctaatgctg taaagacgcg tgttgaaaat
     1501 tttgtatggc gcttttgcat acgatagaac acatataata atgccgcaaa aactagaacc
     1561 actaaaactg tgatgagtgt tgtcattttt ctcctcctca tattattaat gacaagtcca
     1621 tcgaaggaag taaaggttcc cgagaatgat tcttcaacca tcctcctgaa aaaatttttt
     1681 tcagtgactt ttatatttta gacgattttt atcgaaatga cattcttttt gcttacaatt
     1741 agtaataaaa aaacatacct gtgatagctt taatctatcg caggtatgtt tttactgtct
     1801 attcttgttt ttctgattga aaattcaatt ttaccccagg ctgatctaag gcgatctctg
     1861 tcacctcata agacattcgg cgtacgatat cgaataacgg tgctactagc tcgtccactt
     1921 cttcttgtgt gacatcattt tgaccttcaa ttttccgatt gaggatatga ttgatctgac
     1981 tcacacttcc agataaaaca tatgtatcaa acactaaacg gaactctaag cgtgctccta
     2041 cgatgctatg ttctttcgga tagttttcga tcgtttcttt taaaggcgag aagcctactt
     2101 tcaaatctgt ttctacttct tggttcggca atctcatatc ataatgaaat gcttctacaa
     2161 cttctttttg tcttctgatt tccatataat tttatcactc ctttatgaaa attataacat
     2221 atctcctttg aaaggggata ctacttttta atgatgctct gcaaggtaag gatttcattc
     2281 agatttccta ccacataatc tgccgggatc tcacccagaa aatgatcgat gtcgtaaaga
     2341 caagtagcga ctcctgcatt tttccctgct tcgatatcta aacgacgatc gccgatcatc
     2401 atcgtgtctg ttctttctaa atgaaaagta tcgatcaaat aatttaaaga tgctggatca
     2461 ggtttacgag gaaagtcctg atcgatccct actacttcct caatcagatg tgccaatcga
     2521 tgttctttta acaggcccca tgttgattct gtcaaacggt gggtcaggat gaaatgacga
     2581 ccgccgttgt cttttagcat ttccagtact tgtttcgttt ctggaaatgg acgggaaact
     2641 tccgtacttt ctttttcata tcggtgaaat aattcattaa actctctggc attcaagttt
     2701 gattcattga tcaaataact agtagaatac tttttcattt tgaagtagat ttcttttttg
     2761 tccatactga taccaaaatc ttttagtgct tgccatgcac catctaccat tgcgggatac
     2821 gtatcaaaca atgtaccatc aaagtcccat atataatttt tcatgttcct tttgttccat
     2881 tcctttgctt cttttttcta tctctcactt ttagataaag gtgttcttta aagtattttt
     2941 ttgtccagaa ttgtcaaatc atctgctaat gtatactgga ttggttgtgc atttgggata
     3001 tcgatcgtat ccatttgatc ttcaggaaca tcttctaaat attttgtcag tgctcggatg
     3061 ctattaccat gacccacaac tagtaaatct tcccgatctt ttagttttgg tgctagatga
     3121 tcttgccata agggaatgat ccgattcact gtcatttcca gactttctcc gtatgggatc
     3181 aaatgcggat ccaagtgatc atatctgcgg tcaaatctat tttctgcggt taacggcgga
     3241 acttccgtat atcctctgcg ccatgttttt acttgatctt ttccgtatat ttctttcatc
     3301 ttatctttgt ttttacccac aagatcccca taatgccgtt catttagacg ccatgttttt
     3361 attataggaa gccataactg acccatttct tctagtgcga cttgtccggt aagaatcgca
     3421 cgtttcaaaa cagaggtata aacggcatcg aattgaagtc cggcttcaag cattttttga
     3481 ccggcaactg ctgcttgttc ttttcctttt tctgttaagg cgacatctag ccatcctgtc
     3541 cagtaatttt caaaatttgc ttcgctttcg ccatgacgta ataatactaa tttcattctt
     3601 ttctccctta aacattcgtt tcgaatttta taccacatgt tttgcatgtc acacgtattc
     3661 gtccttttcc tttaggtgca cgctgctttt gatgacaatt cgggcactca aaaaaagtat
     3721 aactttttcg atccattttg acttgctttt tgttttttat ctgatgatat ttttctttga
     3781 ctctatcaag atatttcaaa taacgttgat tctcatttag tctggggtag attttttttg
     3841 ataggaaacg aaatatcatc catccgaaaa agacgatgaa caacaattgt ccgattctat
     3901 agggcaacca acgacttagc aaactaaaga caagcgcagc gatcaataga ccgcgattca
     3961 actgatcaaa tttcgcatat cttccctgca taagtttgtg atagttctgc tgaaatcgta
     4021 ttagcctttc taaccatgtt tgcatcatat tatctcctcc atcatctaat atttattatg
     4081 acaaaaaaat ccaaaaagaa aaagccttaa ttggcctttt ccattgcaac ttggtgaagg
     4141 acattccgat ttctggtaaa gtcaatcaca atcactttct ttttatcttc tctgttgact
     4201 actcgaaatg tgtccgtcaa tggaagaaac agcttcttag atggtagctg ttgcatacgt
     4261 ataaaacgat ggatcatcgt aatcaattga ggaaattgaa tagacgtgtg aagcagacct
     4321 ggatattttt tcaacagctg tgtcgttttg atcagattcc gttttttact gatcactttt
     4381 aaaaagcttt ttagaatctt ttgctgacgt ctaaaaatag aaaaagaatt ttcgtcatct
     4441 atttgataac gcatttgttt ttgcagttct gctgcagtca gatggatctt cccatcgttt
     4501 gtatcgatca ttaaaccttc tggaaacagt tcaaccactg ctcgatccca agattcaaga
     4561 gaaagctcga tataatcttt tactggtaat tccaattcta ccttaaaaaa gtgcaaaaca
     4621 gccgacagcc cttctgcttg atagatttct cctaatgtta gcccctctag atggaactct
     4681 ggtgaaaaat gcagcaatgt tttattttcc tgcaattgaa catatagaaa tgtagatcgc
     4741 tcaatcagag tatcttttaa aacaactaaa aaattcattt ttctccccct tttttcatca
     4801 tttttaaaat agcatatttt gaaaagaaac tctgatgctt agagtagaat ttcataaaaa
     4861 tttactattt cataagtatt cacgagaaaa atgcaaacaa aaaagcggaa ataacgctca
     4921 tccgccttct tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc
     4981 tctatcgtta ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat
     5041 cttagtgata ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat
     5101 atcgatgttg atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac
     5161 taatgtcaac acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc
     5221 atgtacacca gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga
     5281 aaagtcgata acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt
     5341 caatgctaca agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt
     5401 ctgtcttttt catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt
     5461 gtcaatgcaa tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt
     5521 tgtgcagcga ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct
     5581 actccaccgt tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata
     5641 aatgctagcc cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc
     5701 cacatagaca atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc
     5761 gatcggattc tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc
     5821 tcagcaaacc ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa
     5881 ggcaatcccg caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact
     5941 ccgtgcagga aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag
     6001 aaacgctttt tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata
     6061 ggtgccactg ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc
     6121 cagaaactca ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag
     6181 caataccgac cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca
     6241 tccctccggc aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg
     6301 aatcaaaagc aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta
     6361 tgtagcctat agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa
     6421 aggctagaaa tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct
     6481 ctccgtcaga tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg
     6541 tgatcacgat cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga
     6601 tcatatattt ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa
     6661 agagcgtcat tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc
     6721 cttctaacgg tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag
     6781 cagcaatcac ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta
     6841 ccttaaaatg aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa
     6901 cataaaataa cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac
     6961 gcgcttattg tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc
     7021 aaaacaaaaa atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt
     7081 ccatagtctg gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta
     7141 tcaggagatt gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg
     7201 atgattgcag ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata
     7261 cttagttctt ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta
     7321 acattttgtc gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc
     7381 aagaaactgt tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg
     7441 atcatcaatg ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct
     7501 tttcgtttac tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca
     7561 aacacagtgg tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc
     7621 tcattggaaa acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc
     7681 gatgtgatat ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg
     7741 gaaataaatg ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga
     7801 agttctaatg cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt
     7861 ttcatcgact ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa
     7921 caataacaaa ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat
     7981 tatagatgta ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt
     8041 gtacttcatc aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga
     8101 ttggttttaa atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa
     8161 tagatgctgc ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca
     8221 tattgtatcc agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg
     8281 gatgagtcaa ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta
     8341 caaggccaga ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga
     8401 tacatggaag aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta
     8461 aaaggttgga aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca
     8521 ggaatgtatt aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac
     8581 ctaacccgtt aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca
     8641 aaaccatcgg aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat
     8701 gcccgttttt gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta
     8761 tctacatgaa aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt
     8821 tcgtttgtct gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat
     8881 tgtgcttcta cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg
     8941 attcgatgaa gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc
     9001 acttctggta tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa
     9061 aattcagaag cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt
     9121 ccgatttctt tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg
     9181 aaatctttcg ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca
     9241 aggttagaag cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc
     9301 cattttccag attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg
     9361 ctagaagcta ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt
     9421 tcgactagat cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca
     9481 ttgtcttgga tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc
     9541 ataatcatcc ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc
     9601 ttttctttga catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc
     9661 tcttctgtat aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc
     9721 gaatcgaaag gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt
     9781 gcaaaatgat cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc
     9841 aaatagcctt gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc
     9901 ccgctcgcta aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact
     9961 tctatctctg tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg
    10021 aatgtctcat cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca
    10081 cttccgcatc ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac
    10141 tttctgatgt tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca
    10201 ttcatcatac caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc
    10261 ctaaaataat aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac
    10321 caaagatcga aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa
    10381 ataagtattt ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt
    10441 aattcttatc gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc
    10501 tgagcacggg tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc
    10561 tttggtacat gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat
    10621 cgaattatcg cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag
    10681 ctatgatgaa tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt
    10741 aaagcctcga ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat
    10801 caaaaaaaag cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct
    10861 ttcacagcag attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac
    10921 cttgtttttc tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa
    10981 aaacagtcga tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt
    11041 tctacctttt cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg
    11101 aagtttgttc aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat
    11161 tcttttgcaa tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga
    11221 caacgtttct atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta
    11281 accaaccacg agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct
    11341 ttagctgtca aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca
    11401 gcttatttga aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac
    11461 gagcatgttg ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc
    11521 gatgctgagt atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc
    11581 cacttttctg caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc
    11641 gtccaggaaa attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt
    11701 tcatatagct atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat
    11761 ctaaaaaaag tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg
    11821 acggaaatct gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa
    11881 gaagagacag gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa
    11941 aaatcaaaga gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc
    12001 tgcaagccat cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc
    12061 tgttcaatca atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga
    12121 atgatacaat cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt
    12181 tttccaatga tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa
    12241 tgaatgactc tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag
    12301 aaaatggaag aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt
    12361 ttcaatctgc gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg
    12421 cacaccacgg attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac
    12481 tgaaaaatac attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac
    12541 tagcgaatct ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg
    12601 aagaacagac gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt
    12661 tcaaaaaact aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc
    12721 ctctgtaccg ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc
    12781 aaatgggcgt gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct
    12841 cattggtatt tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc
    12901 taaaacggct agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag
    12961 tccaattgct agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac
    13021 agacgatcca agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga
    13081 tacagcccca ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa
    13141 aaacagatga agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg
    13201 ggaaaaaaga agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg
    13261 atcttttgag tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac
    13321 tgcagtcatt atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc
    13381 atgcttttca caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc
    13441 ttcttttatt cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa
    13501 aaaatgatca tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt
    13561 atggcgatgc atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga
    13621 tttgtctacc gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc
    13681 gctgattttt ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg
    13741 tctcttctac tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct
    13801 tgatttcttg gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca
    13861 cgaaaaaata ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga
    13921 tttcccgcca gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta
    13981 gtgtcaagat gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac
    14041 cgacgatcca aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct
    14101 ccaatccaac atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat
    14161 gttctgctac atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag
    14221 gataatgcgt ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt
    14281 ttgtaatctt tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac
    14341 ttttaacttc gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt
    14401 ccaatgaatt ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca
    14461 aacaaacagg gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg
    14521 cctgataagt gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct
    14581 ttctataatg gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt
    14641 cgcactttct tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt
    14701 ttttccaaga gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca
    14761 gtccgcttct gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc
    14821 tgcactttgg acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct
    14881 taaaatatct gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc
    14941 aatcgacaat tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa
    15001 acattgagct aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa
    15061 aatgctagcc atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg
    15121 gataagagat tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag
    15181 cagctacttg cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat
    15241 gcagaggtgt cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca
    15301 ttttctgtaa ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt
    15361 ctgcgtcttt gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca
    15421 attcatcaat cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg
    15481 tcttgacact taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg
    15541 cgcccatctc ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt
    15601 ttaacaacag ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg
    15661 aaatatcacc tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga
    15721 tcgttttatc ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt
    15781 tacgggaacg ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca
    15841 gcaaagcaga tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag
    15901 agcgggttcc tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag
    15961 cgcccatctc gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat
    16021 ccccgaacag agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg
    16081 tcgtaccgga gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc
    16141 gaccatgaat cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg
    16201 catgtaaagt actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt
    16261 cagcaattgc tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa
    16321 cagtccctct taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga
    16381 tccagcaatg ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt
    16441 ggtttttaaa actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca
    16501 aattaatcaa agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag
    16561 gttcatctgg cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga
    16621 caggcaactg gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt
    16681 tctcagtcat gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt
    16741 ccaataatat ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag
    16801 cgatcctagt gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt
    16861 gattgaattg ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta
    16921 gcattcctgt tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc
    16981 cgcaaaatag ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga
    17041 tgtaataggc attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac
    17101 cagccattgg atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt
    17161 gctgcttcgt actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa
    17221 ggacatcaag ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt
    17281 gttctgcacc tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt
    17341 gctcacgggt ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac
    17401 gaatacaaag tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg
    17461 ccatcttatt tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca
    17521 gctttcaact caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca
    17581 gtggctacaa cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt
    17641 tctacacttg ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac
    17701 aatgtaggaa tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg
    17761 ccttcgaagc cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg
    17821 atctcatcca tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct
    17881 acgcctttaa aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg
    17941 tcccattgta cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt
    18001 cctccgatcg tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca
    18061 actgctggat caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc
    18121 gagatatttc caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg
    18181 atgatgtcaa gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc
    18241 gtctctcttg cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata
    18301 ccgcctacca aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct
    18361 ttttcaggca cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct
    18421 accactaaag tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga
    18481 acacgatctt tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg
    18541 agttcgtgat tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg
    18601 gctgttaatt ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc
    18661 taagtaatct ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc
    18721 tagcaagatg atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa
    18781 taattcatct ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct
    18841 ttctaaagca gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat
    18901 ggctaccatt ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt
    18961 ttcaattgca tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa
    19021 gacatcttct tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc
    19081 atgttccaga agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc
    19141 tgctatagct gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa
    19201 agtttttaga acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag
    19261 gtttttcgct ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc
    19321 aagtagcgta gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa
    19381 tgctgctaga tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa
    19441 atcattatct gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc
    19501 acccgccggt acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat
    19561 tttttctgca tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct
    19621 ccaaatcgtt tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc
    19681 gtatttttgg ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc
    19741 tggaattaat cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc
    19801 atcatgcgtg agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca
    19861 ggatcaggat ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc
    19921 attggtggta caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa
    19981 tgagttaatt ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt
    20041 tcgtaagtcc gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg
    20101 tattctgcag cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca
    20161 ggtttacctg ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca
    20221 atctgtagga tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca
    20281 gtaatcactt tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct
    20341 tctaaacctt ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt
    20401 tttgcgcccc cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata
    20461 gaacaaggac cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc
    20521 acgactgtat tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc
    20581 acagcatttt ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca
    20641 ccttttagtc caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct
    20701 ttttcgatat gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt
    20761 actatcattg tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta
    20821 ctttttcaag gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt
    20881 ttcgctcctt ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg
    20941 aaattgcctc tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa
    21001 tagccactat agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca
    21061 tcatagatac aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa
    21121 atagctaaag taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc
    21181 caaactgatg taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt
    21241 tatgaaactt tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg
    21301 ttttattgaa gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc
    21361 atgacatata gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa
    21421 agattgctac tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg
    21481 tgactcaaca tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat
    21541 cgtaagaacc gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc
    21601 ttctgctttg aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa
    21661 cgaaaaatct tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac
    21721 ccatttcgtt tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag
    21781 tcctgccact ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc
    21841 tgataggcct tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa
    21901 tgcgggtaat tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc
    21961 ctctccagcg atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag
    22021 tgtaaaaatg atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg
    22081 agcaatcggc aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg
    22141 atacaatccg gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt
    22201 atcggtcgtt gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt
    22261 tttattgagc gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga
    22321 tttgacacct aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact
    22381 tttatttact gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc
    22441 catctgcaaa aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt
    22501 caccgccata gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat
    22561 ctttaaaatc ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt
    22621 aagaatccat tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg
    22681 gtttttccgc tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc
    22741 tttttacatc tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga
    22801 caagtcgatc ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg
    22861 cataagattc atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga
    22921 aattcgtttt tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt
    22981 tgtaagaagg ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt
    23041 ccgccatcac atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt
    23101 aatgagatgt ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca
    23161 tatcttctat agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt
    23221 tgataatcat ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa
    23281 aaccgaagga atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt
    23341 caagcttata taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga
    23401 tgcgaatcgt catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag
    23461 gatctagtat cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta
    23521 cttcaagttt gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta
    23581 aatggttttg tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa
    23641 agtcctgatc ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat
    23701 tttcttcttc caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat
    23761 acccgataaa atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa
    23821 tcgagatttg ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa
    23881 ccggattgtc tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga
    23941 aagggggacg gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct
    24001 tgtcataatt tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat
    24061 caaaatcttt tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac
    24121 gccatgatcc atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg
    24181 cataacaaac atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat
    24241 ctaaccattt tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg
    24301 aagcagttct gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa
    24361 ttcggccccc gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa
    24421 ccccatcaga tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa
    24481 cacaatagct tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa
    24541 aggactcgcc aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga
    24601 tataatttgg tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc
    24661 ataaccaaaa acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc
    24721 ggtctgatga tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag
    24781 gcttgttcca tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga
    24841 ctgcggcata taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc
    24901 ccataagatt tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat
    24961 ataacgatgc gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga
    25021 aagcgaacgg ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct
    25081 catttgtttc tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga
    25141 tctggaagca gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata
    25201 cagttcagaa tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt
    25261 gctatgagca taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa
    25321 ccagtctcct aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac
    25381 gaacatttcg atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt
    25441 gaagtcaaag taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg
    25501 acgacctctt gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa
    25561 tccctgactt gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa
    25621 ttcccacgaa aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc
    25681 tttttcaagt tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc
    25741 agtattttct gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct
    25801 cgttcctcga cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg
    25861 tctttcattg acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc
    25921 aaagtctagc tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct
    25981 tatctttaca gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa
    26041 ataaatcgct gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc
    26101 catcacataa gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct
    26161 gtcatatctc agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt
    26221 tcgaaagtcc atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga
    26281 ccggtcagga tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt
    26341 attgtctttg tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc
    26401 ttctatcttt atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc
    26461 atcaaataag atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc
    26521 aacaaaacgg aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt
    26581 ttgtcaataa aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat
    26641 gtcgcacgaa aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag
    26701 acgatccatg tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat
    26761 tatgacatca gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct
    26821 acgatcggca aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg
    26881 gaagaagaag ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat
    26941 cctaaacaat gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg
    27001 aaagaatcga ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct
    27061 gacatccagc aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta
    27121 ctggaattgt tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc
    27181 gatatggatg aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat
    27241 cagcatttta tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg
    27301 acagaaaaac aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct
    27361 actgccattc ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa
    27421 aaaaatatcc aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc
    27481 gcaaacctct cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca
    27541 gctgttcgaa tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact
    27601 tatcctgcac gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag
    27661 agaggttgtg aaa
//
LOCUS       DAZKSU010000035        27602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000035 DAZKSU010000000
VERSION     DAZKSU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.035"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..650)
                     /locus_tag="ACG8Z3_001520"
     CDS             complement(105..650)
                     /locus_tag="ACG8Z3_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0823838.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(662..1354)
                     /locus_tag="ACG8Z3_001521"
     CDS             complement(662..1354)
                     /locus_tag="ACG8Z3_001521"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0823839.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1373..1657)
                     /gene="macP"
                     /locus_tag="ACG8Z3_001522"
     CDS             complement(1373..1657)
                     /gene="macP"
                     /locus_tag="ACG8Z3_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0823840.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1670..2233)
                     /locus_tag="ACG8Z3_001523"
     CDS             complement(1670..2233)
                     /locus_tag="ACG8Z3_001523"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0823841.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2461..3123
                     /locus_tag="ACG8Z3_001524"
     CDS             2461..3123
                     /locus_tag="ACG8Z3_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0823842.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3247..4443
                     /locus_tag="ACG8Z3_001525"
     CDS             3247..4443
                     /locus_tag="ACG8Z3_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0823843.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4544..5920)
                     /locus_tag="ACG8Z3_001526"
     CDS             complement(4544..5920)
                     /locus_tag="ACG8Z3_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0823844.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5957..6823)
                     /locus_tag="ACG8Z3_001527"
     CDS             complement(5957..6823)
                     /locus_tag="ACG8Z3_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0823845.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6912..7937)
                     /locus_tag="ACG8Z3_001528"
     CDS             complement(6912..7937)
                     /locus_tag="ACG8Z3_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0823846.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7955..8629)
                     /locus_tag="ACG8Z3_001529"
     CDS             complement(7955..8629)
                     /locus_tag="ACG8Z3_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0823847.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8813..9706)
                     /locus_tag="ACG8Z3_001530"
     CDS             complement(8813..9706)
                     /locus_tag="ACG8Z3_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0823848.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9780..11477)
                     /locus_tag="ACG8Z3_001531"
     CDS             complement(9780..11477)
                     /locus_tag="ACG8Z3_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0823849.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11470..12783)
                     /locus_tag="ACG8Z3_001532"
     CDS             complement(11470..12783)
                     /locus_tag="ACG8Z3_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0823850.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12783..16724)
                     /locus_tag="ACG8Z3_001533"
     CDS             complement(12783..16724)
                     /locus_tag="ACG8Z3_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0823851.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16833..17291)
                     /locus_tag="ACG8Z3_001534"
     CDS             complement(16833..17291)
                     /locus_tag="ACG8Z3_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0823852.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17589..18107)
                     /locus_tag="ACG8Z3_001535"
     CDS             complement(17589..18107)
                     /locus_tag="ACG8Z3_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0823853.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18109..18726)
                     /locus_tag="ACG8Z3_001536"
     CDS             complement(18109..18726)
                     /locus_tag="ACG8Z3_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0823854.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18723..19577)
                     /locus_tag="ACG8Z3_001537"
     CDS             complement(18723..19577)
                     /locus_tag="ACG8Z3_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0823855.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19574..20485)
                     /locus_tag="ACG8Z3_001538"
     CDS             complement(19574..20485)
                     /locus_tag="ACG8Z3_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0823856.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20526..21860)
                     /locus_tag="ACG8Z3_001539"
     CDS             complement(20526..21860)
                     /locus_tag="ACG8Z3_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0823857.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22068..22898
                     /locus_tag="ACG8Z3_001540"
     CDS             22068..22898
                     /locus_tag="ACG8Z3_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0823858.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23142..23391)
                     /locus_tag="ACG8Z3_001541"
                     /pseudo
     CDS             complement(23142..23391)
                     /locus_tag="ACG8Z3_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23582..23776)
                     /locus_tag="ACG8Z3_001542"
     CDS             complement(23582..23776)
                     /locus_tag="ACG8Z3_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823859.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24281..24466)
                     /locus_tag="ACG8Z3_001543"
     CDS             complement(24281..24466)
                     /locus_tag="ACG8Z3_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823860.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25316..25657)
                     /locus_tag="ACG8Z3_001544"
     CDS             complement(25316..25657)
                     /locus_tag="ACG8Z3_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0823861.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25657..25887)
                     /locus_tag="ACG8Z3_001545"
     CDS             complement(25657..25887)
                     /locus_tag="ACG8Z3_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0823862.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26174..26512
                     /locus_tag="ACG8Z3_001546"
     CDS             26174..26512
                     /locus_tag="ACG8Z3_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823863.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26717..27279)
                     /locus_tag="ACG8Z3_001547"
                     /pseudo
     CDS             complement(26717..27279)
                     /locus_tag="ACG8Z3_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         8201 a         5882 c         4712 g         8807 t
ORIGIN      
        1 ttttttaaaa taacgtataa aaaagagcat aaaacaaagt cgtttgtttt atgctcttct
       61 tttattattt tgtgcttttt attttatcaa atcactttgt ttttttataa aatttctgcg
      121 cctagtgtgg actcaaaatg tctcaatgcc cattcatgtc caacgggatc gaaacttgcc
      181 acggcatctt tgaaaatatg gagtttgtat cctagattat acgcatcgac tgctgtatgc
      241 aacacacaga tatctgtaca tacgccggtc aaataaatat ctgtgatctg gcgttctcgc
      301 aaacggatgt ctagatctgt tccactaaaa gcggaataat gccgtttgtc tatccaataa
      361 acattttcct cttgctcata gtcttggtat aaacttttta aagagccgta caattcccgt
      421 ccacccgttc ctatcacatt atgcggagga aataagcggt tctccggatg aaaggaatct
      481 tttggatcat gcgcatcaat ggcaaaaacg acaaaatcct tttgatcaat aaacttcttt
      541 gtatgagcag tcagcttttt ttcaattact tgtcctgctg tcccagtagt cagctttcca
      601 tcctctgcaa caaaatcata tgtgtagtca atcgagatca atgcgttcat cttagtcctc
      661 cttatacgag atgtttgaca aattcgatca ccatttctgc tgatcgtttg cctgcgtctt
      721 cgataaattc gtcaaagctt tgtgtcgccg agtgatctgc tgtgtcgctc attgcacgta
      781 cgatcaaaaa tgggatattg aactgacgag ctgtttgtcc aacagcggct ccttccattt
      841 cgcaagctaa tgcttcaggg aaattcgtta agatttcttt gattttatct ggcgagtcga
      901 caaatgtatc acctgtaacg atcaatcctt cttttgcgtt caaattcgtt gcttttgctg
      961 ctttaaccat ttcactgcgc aaatatgtgc ttgcttcata atatagaggc attcctggta
     1021 attgtcctgg gttgtagcca aatcctgttg catctgcatc aaaataagct actttgtctg
     1081 atataacgat gtcacctact tgtaagcctt caccgattcc gccagctgaa cctgtattga
     1141 tcaccatgtt cacgccgtat tgttggatca acagacttgt ggtaatagat gctaagactt
     1201 ttcctatacc ggaacgtacg acgatcactt cgtggttacc taatgaacct gaaataaata
     1261 aagcgcctgc gcgttcccat gataatggtt cgcttaggtt ctctctcaaa atttttactt
     1321 cttcttccat tgcgccgata ataccgattt tcatcttttt aattccttct ttctatataa
     1381 atgcgattgc taaaaaaacc acacataaca ttaggataac aataataaga gatttcatca
     1441 aaaaagagtt ccatttggtc gtcttttccc gttcgctgat ccttgttttc gtgattggtt
     1501 tattcttttt gctttcttta cgataaaagc tggcaatctt tttttcttct tgctgataag
     1561 ctgtttctgc ttttcgctgt ttttttaatg actcgcttgc ttgtgcttgt tgtcgcttgc
     1621 gaagttcgct tcgcgtaata agcggtcctt tggccataag ccgatccctc tactttcctt
     1681 tggtcaatag ttcccagtat tgaatcgcat aaatcgtctt tgcatcacaa atcaactgat
     1741 ctttcatcgc ttgttgcgcc tcttcaagag ttagatgata caactccagt acttcgtctt
     1801 catcttgtgc taaaggattc tcgaccttct ctaccccttg tgcatgataa atatgaagga
     1861 cttcatttgc aaaacctggt gaaagataca tcgaagtaag atggctcagc gacttcgcac
     1921 gatagccagt ttcttcttct aattctctag ctgctgtcat ttcgggattt tgtccttctc
     1981 ctggatcgat ttttccagct ggaatctcca aaatcacttt ttctaaaggt tttcgaaatt
     2041 gtttgacaag aagcagacga tcttgttcgt caaaagcaat gatcccgaca ccgccaggat
     2101 gaaataccag ttctctcttc gaagttcctc catctggcaa acgaacttcg tctacagcaa
     2161 catcaataat ttttccgcta tagatttctt tgcgactgat tgttttttct tcaaattgct
     2221 cattgttcag catatcttct cctgctttct ttgtaatcat aactgctttt ttttcatttt
     2281 tcaatttttc tcaaaaactc cttattagtt taccatagat aaaccttaga ttttctctaa
     2341 atatagaaaa tccttcttta gtccgatact ctttatagta gtccgtatgc taaaataagt
     2401 atgtaatcga ataacgaaaa gaattcaata ctcaacgata aatgacatgg agggtttatt
     2461 atgataaaac gaaattggcc gtggcttcta gcattggtct tgctatttct ctttgtccga
     2521 aacaatagtt tttcttttgt tctaggactt ttattgatag gtgtagtttt agttttgtgg
     2581 ggactctttg gttcgcgcag gaaaaaggga aaacaagaag aaattcctgc actgactgac
     2641 gaacttgaaa ctcactatga aaaaagcggt atgacagcga atgaaatttc attttttcga
     2701 caaacaatga atcaaacaaa aaatgagatc aatcaattgc aaatgaatat gcagcagaca
     2761 gccaaactta aagcaatcga tctccgccat gatccagtca aagcagcgaa agctttattc
     2821 aaagaattgg tgaaagaacc aaaccgactg catgaagcaa gtcagtttct ttatacgcat
     2881 ctgccgaatc tagttgattt gacaaataag tataatgaga tcaatgacca tgagatcaaa
     2941 aacaaacaga cctatgaaaa actagaagaa agcgcgcaga tcatcgacca gctgtcagct
     3001 ttaatcgcac aagattatca aaaattcgtt gctgaggatc tagatgatat cgacgtagag
     3061 atatctgttg cgaaacagag cttaaagcgt gataataaat atgaaccaga acaaaaggat
     3121 taactctttt gttcataaaa gaagactcgg aacattcaga tgatgtctgg ctgtttcgag
     3181 tactttttct aaagtagaga cattcatgta agaatcgaga acagattcaa caaggaggaa
     3241 ttttttatgg atcataaacc gactgaaaca aaagatgtta caccagttaa cgatacactt
     3301 gaagatttat tgaacaaccc gttttcaact ccaacagata cgctgaccac ttcacaacaa
     3361 gcggagattg atcagctcca agagcaacag acagctgctc gtttaattga caaactaccg
     3421 gcggaacgcc aagaacaagc taaacagcta gcagcaaaaa tcgatgctaa tgatgcacag
     3481 tctgtcatca gctacggatc ggctgcccaa gcaaaattaa gtgaattctc acaatcgatg
     3541 ttgaatcatg tgcaagcaca agatattggt cctgtaggag attccttaac tgaactgatg
     3601 tatcgtctcc aagaagccaa tcctgacgaa ctgcgtgccg gagaagggaa catcttccag
     3661 cgtgtcttcg ggaaggtcaa acaatcgatc tatgaagtga cagcaaaata tcaaaagatt
     3721 ggtgcacaaa ttgacaagat ttcagtgaaa ttagataaag aaaaagacgg tctgctaaaa
     3781 gataatctga tgttagaaca gctttaccag aaaaacaaag actactttga tgcactgaat
     3841 atttatatcg ctgctggaga attgaaaatg gaagaacttc aaacaacgat cattccagaa
     3901 gcgatgaaaa gagcagaaga aactggcgat cagatggatg ttcagattgc caatgattac
     3961 acgcaattcc tagatcgtct ggataaacgt acgcatgatc ttcgcttagc tagacagatc
     4021 acgatccagc aagcaccaca aatccgtttg atccaaaaca caaatcaggc cttggctgaa
     4081 aagatccaag catctatcgc tacagccatt ccgctgtgga aaaaccaagt cgtgatcgca
     4141 ttgaccttgc ttcgtcaaaa agatgcagta actgctcaac gccaagtctc tgaaacaaca
     4201 aacgacttat tgaagaaaaa ttcagaaatg ctgaaaatct cagccatcga aacagcgaaa
     4261 gaaaatgaac gtggaatcgt ggatatcgag acccttcaaa ccactcaaaa tgatttgatc
     4321 gaaacaatcc aagagacatt gcgtatccaa aaagaaggaa aagaaaaacg tcgtcatgca
     4381 gaagtagagc ttggacacat ggaagaagat ctgaaacaaa aactgttgga tttaacccaa
     4441 tagatcacct ttcgcttgtt actaaaacaa acgcccaaca acagtttatt acaactgttg
     4501 atgggcgttt ttcttatctt tcttctaact tctccattgg agtctatttc tcattcatct
     4561 tgctgtgtcc atagccatag ccaaaataaa tgatcagtcc aataaccatt acgataccga
     4621 acacgatcca tgtcacagtt tgcagacgaa gcatcaatac aatacaagtc aagatggaga
     4681 cgattggcaa tagcggaacg aatggcactt tgaattctcc agcttttggt ttgccaaaca
     4741 tttttctcaa cttcagtaag cctagactaa caagcatcaa ataggcgagc gccatgatat
     4801 tcgttagttc cgctaataca ttcaatggga aaaatccggc aaagaataca gctgccacac
     4861 cagcggcata agtcgctttt ttcggtgtac gatgcttttg atcgatttct gtcataaact
     4921 taggaagtaa gccatccttg ctgacagcaa acagtaaacg agcaagtgaa tacatcatcg
     4981 agatcgttac cgtcagtagc gttaaaattg cccctacagc gatcaccgcg cccactgttc
     5041 catgtcctac atacctcatt gcaaaagcca ctgggtcatt gactcctagt tcagtatacg
     5101 gaacgattcc tgtcaagatc agcgtcacga tgatgtaaag gacagtagca atcagtatcg
     5161 aaccaatgat tcctcttggc acatcttttt gcgggttttt gacttcctcg gctgccattg
     5221 caacggcatc aaatcccaag aaagcaaaaa acacaagagc tgcaccatct agcacaccgg
     5281 aaaaaccaaa aggcatgaat ggctgccagt tatcaggttt aacgaaaaat attcctacaa
     5341 tgataaacaa cgcgatgata ccaaatttga catataccat ggcattattc aaacgtaatg
     5401 ctttcttggc ttcaagcgac acgatgtaag tgacgaatat cactacgagc accgcaatca
     5461 agtcaatgta tgtaccattt tccggattgt acccgccagt taatgcttgt ggaagttcgg
     5521 cccctaaact tttcaaaaaa ccttgcatat agccagacca tccggaagca acagaggaaa
     5581 ccgctagtaa aaattccccg atcaacaacc accctgtcag ccaggcagca aattcaccaa
     5641 atacaacata cagataggca tatggcccac caatcactgg cactcgcgaa gcaaattctg
     5701 cgaaacttag tccagacaaa atgacaacga ttgcagctaa gacaaatgaa agtgacagtg
     5761 ctggaccagc attttgattg gccgcaaccc ctgtcaccac aaatattccc gtaccgatga
     5821 tcgcaccaat tccaagcatg atcaaatcag acgtttttag ttcttttttc atctcactcg
     5881 tatgattgag attgatctct ttcttacgaa acaaatccat tgaagttcct ccccatttta
     5941 ggttggaaaa cagcgattaa actgttttca tataccctaa aaggatacca cctaaaatga
     6001 ctaaaaggca accaacaata acataaatga gctctttttt cgtttttctt tcgccaagga
     6061 tgaaaatccc acctaaagta gagataatga tccccatttg tgaaagtgag aagccaacag
     6121 ctaaaccaag tgatttgact gttagaagca tgcagatgtt tcctaagccc cataataatc
     6181 cacagatcat gtttttccac acaaactgat cgactttaac tttacggaaa gcaaagaagc
     6241 ttgcgcctaa gatcattccg atactttgag gtaagataat tgacatcgca tccaaattcg
     6301 cccagttgac gatgatggta tatacaccat aaccaatcgt agagaagatc aatgctctaa
     6361 aacccttacc aaaatcaagc attttattat ctgtatctat ttttccttcg tcatcttgtc
     6421 gggcagtcaa atacgcaccg cttacaagaa gagctaaagc aataataccg tatacataat
     6481 ctttcgtttg tgtccattca tggaagaaaa cggctcccgc aatcgtattc aaaattaatt
     6541 gaaaaccagt agacaatggt agccctacag aaacacccat gtacttcatt ccatggaact
     6601 gtccattttg tccaacactc caagctaagc cggataaaaa tccgatcaag aaaatccaag
     6661 gggtgattac aggactaaca acgaaaaaga caactaaaga aaacagaaat gcaccgatcg
     6721 tcattcctag tgtttgttga ttggcatcgc caccaatttt accacttact aaaccaatac
     6781 taccccatgc caacataggg actaatgcta ttaaaatttc cataaccaat ctccttttat
     6841 ttcacatgat aagaaactta tcagcgaaaa cgttaacaac acaagcactt tttttcgttt
     6901 ctaacgattt tctaacgttt ttcattataa aaatttaaga ttgggaaaaa taaagtcatt
     6961 gttgtccctt ttccttcagt tgattcagcc gtcaaatgga tacctaattt ttcggccaga
     7021 ttttttgcta aataaagtcc taatcctgtt gaatgtgttt tgctaagtcg accatttctc
     7081 ccagtaaatc ccttatcaaa gatccgtttc aagtcttccg ctgggatacc gatccctgaa
     7141 tctttaactg aaaggctgac tccttgcgct tccttttcta tgctcacaat gatgtttccg
     7201 tgatccggcg tatatttgat ggcattacta agcaattggt tgaagataaa agcgatccat
     7261 ttagcatcgg tcaaaacagt atgatcctca ccaacgatct cgtagcgaat atttttttgg
     7321 ataaaataat ttgcctgact gcgtatcaca ggctgaatga ttccttttag tccatattct
     7381 tggatgaggt agtcccgcga gaaactgtcg agtcttgcat aatacaaaac ttgttctacg
     7441 tattcatcga tttttgataa ttcattttct agtaatgtat acttttgatc gtcaatatcg
     7501 aactcgatcg aacgaactag caatttagaa gcggaaagtg gcactttgat ttcatggacc
     7561 catgagtcaa tataatcttt ttggtcttgt tgattactga ttgctgattg cattacttct
     7621 tggtgttcac ggatcagctg attgatatat tcttctacca gtttttcttc ttctgtcctc
     7681 ccaccactca aataattttg caaaggggaa gttttttgta tgtcggctaa ttttccccac
     7741 catcttctct tgctcaagta accacccagc agataaaaaa tcagaaagac gccctcaatc
     7801 aaagctaaat aagcaattgt gctcaaatca actttgatgt ttggcgccag ccacatcaca
     7861 aagatcgtca atgcaataaa aaacagccat cccaagatca atgtccactg atctttcaaa
     7921 tatttaaaaa aattcatctt aaccgctcat cctttcaggg aacgatatat ccctgtccta
     7981 cttttgtttc gatatatcct tggatacctg cctgttcgat ttttcggcgt aaccgattga
     8041 tgttgactgt caatgtattg tcatctacaa atcgttcgtc atcccacagt gctcgcagca
     8101 atttttcccg acttaagatc tttccattgt gcttcatcaa aataaataat agacgatatt
     8161 catttttgct caaatcaatg atctctccgt tgatttccat acttccgttg tcaacattca
     8221 acgtaatgcc attgtggctc atcatttcac tagataattc tgtgtagttg taagaacggc
     8281 gaagtaatgc attgattttt gcaatcagta catcgatttg gaaatgtttt gtcacaaaat
     8341 catctgcccc catgttcata gccatgatca tatccatatt cgtattcctg ctggagataa
     8401 aaatgatcgg tactttcgat acttcacgta ttttttgaca ccaataatag ccatccaaaa
     8461 cgggaagatt aatatctagt aatacaagct gtggttcttc tctttcaaag tctgagaaca
     8521 cttgattgaa atcggtcgtt ccaaaggttt caaattgcca tttctcaagt tcctccatga
     8581 tcaattgtcg aatgatctct tcgtcttcga ctaccatgat ttttgccata aaaaatccct
     8641 ccatctttct tcttgctact acaatatcaa agaagccctt gtgaaacaag ttggcttacc
     8701 tggaaaatgt cattttagca aacgttaaaa aaaactggaa atatgcgatt taatcgcgtt
     8761 tgtctcttcc aagcagctta aatttattat gttattctta ataattggtc tattatctgt
     8821 tatttttctt tcgttgtttt tgtcttttaa attttcgata acaccaataa ccgaaaagcg
     8881 ttagcactag caatatacct agaaggagta taagtaatcg ataaagatgg tatttttttg
     8941 tctgttctat ctttgaagag acttcttctg taaagggaac tcgtcttcct gtcactagta
     9001 atcggtgagt gttcacggtg taaggtgtac aagtgataag tgtgactaga tcctgctttt
     9061 ctctaattgc taatgtattt gtttttgttg gaaggacaac aattttctca acgacttcat
     9121 aagcgagcat ccgaccatac acttcaagat agaatcggtc atcttgctcc atttcttcaa
     9181 gatccgtaaa gagttttttc tcagcaatac cgctatgggc ggtaatcacg gaatgtgtac
     9241 tctctcctcc agttggaaaa gaggtgcctt gcaggactgt cgctcctcta tctaacaaaa
     9301 gatcattcgt ttcatcaaat aaaggcaagc taacattgat tttcggaata tagatggctc
     9361 cgatcatatg ttgttcgtaa tattcttttc ctggattttg gacatttcta accgcttgct
     9421 caaaaggatc tttgacttgt cccattcctg ggattactga tgtatgagca agcgtctgat
     9481 ttttcttttc atgcactgct aattgttgct catgtttttc ttctttatcg cgttcaagct
     9541 gcttttgata attctcgatc cttctttgat ccaaataatt gctgagagca tctgctaaaa
     9601 atggatatgt aaaaactgca gctccggcaa gaaacatcac tgccataaat aattgtaata
     9661 aaagactgat ttgggtttga ggtctattac ctattttctt tttcattttc ttcaccttct
     9721 tatttaaaaa aactgcagca acctttataa aaccgattgc tgcagcttat ttttgttgct
     9781 tatacttcag acttcatttt agatttacgg taccagctgt acgctccaat catcaaactt
     9841 attccgatta gtaagaatgc caagagcccg ttcccaccag tagcaggaag tgacccgcgt
     9901 ttcatattcg gtactctttc tggccctgga ttttctactc ctacgatctg aatttcttca
     9961 ttatagcttc cataagagat gataaattca gtaaaggctg cctcacctgt tggaagtaca
    10021 taaccttctg gcgccatggt ctctttcatt tgataggttc cataatctaa cccttgaacc
    10081 tcaaagtatc catttccatc tgcgacaaat tctgttgctt gtccgcttgt tgcccactca
    10141 attctatctg ctcctgattc ataaacagct aaaggcgact cccctttata aaatattgcg
    10201 tattcagaac ggtcttcatt ccacaattcg aatcttgcat ctttaagcaa ttcactgctt
    10261 gatgcatcat atttttcaaa ctttcttcca cctgttgtga ctttctctgg cggctctact
    10321 ccttcttcat aatcaaattc atcatttcgt ccgatttcaa taaccgctgt gttgttatgg
    10381 aaatcatccg cttctagttc tgtggaaacc ttcaaattat acgtgatcgt taaatctcct
    10441 ccacgtgcat ctgctaatct acctaaagta tctactttgg ctactccatt ttcttctgtc
    10501 aaaatcgtca cgacaaaccc gttgcttact acttctacta tataatcttg tccttttgtt
    10561 aaaatcgtct cgccggctcg cacttctatt tcctgatcat aataagttag accaactgta
    10621 ggcgtatctg tgattttgta atttttgata acagcgcttc cgttttcaag gacagaatcg
    10681 atgaaataag gaataggtgc tgtgatcgtg taagaagccg tttttcctct ttctagatca
    10741 atataagaaa tcgtttcttg ttctccagct tcattaataa tcgttacttg tctaaggtcg
    10801 ctttcctcta cattcatctg tttatctgct tctctgattt cgtttttcgg atacaaatgc
    10861 acatctgtat tgtatgtgtc tttccatgta gaaccatccc acacgccatc caccatatca
    10921 ggcatcatca ctggcatcat aacaaccatt ggagacgcga ttctggaaat atgagctggc
    10981 gaataagttt ctaagaaaag atagacttta tctctagaag aaggtgcttc ttccgtcact
    11041 tggacggtag agaataccgc ttctccagct tcattcgttg tttgtttgtc aatacttgtc
    11101 agtcgatatt ttaaaaacca actttctgtg tgccgttttt gaatccattc aatcgcttcg
    11161 gcagaattaa gaccgtcttc tcgttccggt gttaaaggat tatctcgaat caaatcgtaa
    11221 tatacttccg ttacgtcaaa caaatcaaaa tcgactcctg gaatattttc ttcaccaaaa
    11281 tccgtagaaa ccaatccgct attcggtcgt tcttccggaa cagatgagaa accttttttg
    11341 tgtaatgtaa tcgtcacttc acttggtttt tgtgcgactt cttcggcttt gactagtttt
    11401 ccgttcccga aaatccctat tcccgcagta acgagtaaca gtacactcat tcccgtgaca
    11461 atcatttttt tattcatttt tttctccccg ttcttttttt attaataaaa cagcacctaa
    11521 caataaaaga attcctagta aagaaatcaa cgttcttgct tcattcgttt gcgggaatac
    11581 ggaccatttt cctgattgac ctggattgct tggaccactc ggctctttcg gtttgtccgt
    11641 tgtttctttt acggtctcgt tataaacacg tggtgttgcg gattcatagg cagagactgg
    11701 aactttcccg gattctaatt cagccatctc ctgtccgtta actactacat attgtggttg
    11761 gccttttgca tccacccaaa attcagggat gatgacctcg attctcttac ttgcttcatc
    11821 catctcgtaa ccttccacac cttttaattc ttcaaaataa taggtgccag aaggcaagaa
    11881 acgttgtcct gtcgtaacaa ggccctcttt atccgaaatg aatttgctta catttttgtc
    11941 attcaaagga tcattgtctg ctggtttgat ccattgattt ttcaagtcat tagcaggagc
    12001 catatctaaa taatatttcc ccccttctat catctgatat agagcaaaaa cagcaccttc
    12061 taatggtttt cctttttcag ttgttcctct ttgacgtcca tacttgaaga agtatggatc
    12121 tcttagataa ccgacatttt taggatagat atgaatctct tgcagttcct cggttgattc
    12181 tgtcgggtga taaattggca aaatagcagc aattgggata gctgtctgat cgatatcaac
    12241 atccaatcca acttctggat caatcgattc ttcgaaaatc aaataaactg catcccgatc
    12301 ttggtttttc ttaggtgctt tgatacgtgc aatcccgtct cctaatccgc caaccgtatc
    12361 tttcccagtt gttaccgaag tccctatttg gacaagcctt tcttctgtga ttagctgacg
    12421 gatattgttt ctgttcgttt cagcgatttg ctggacgaat tctttaggag tcatgttttc
    12481 agcagctttt ttttcatgat aataatcggt catgtcgtaa atggcaaatg tcgctccatt
    12541 tagtggcacc gtctgtgaaa tgatatctgc tgtttcccct gcttcctcag agatagccaa
    12601 gccatcattt tcatggtatt caaattgatc gttataatcg atatctcgaa tcatccgttt
    12661 atgcaacacg aattccacga catcttcccc tgattcttct gccgacatcg catgtggaag
    12721 aaggactgct gcaagcggta gtaaaacaag taattgaagc atcaaacaca acagcttttt
    12781 cattaccttg ctccctcctt tttagattgt ctgacaaaca aaccataggc tgctcctgca
    12841 agcgccatga agaatgcagc aagcaggata tagaataatc gtccggatcc tcctgtagca
    12901 ggcaacggat tttcaggatc gttcccgaca ctaagctgta atctgattcg atttgtctcc
    12961 aaaagttcat attctaactc ttgctgtaga tcaggcgtct ctgcttcttc gtttggataa
    13021 tacgaaatct caacttttag tgtacccgct tcttttcctg gaactacccg atcattttct
    13081 atgtcaggtt cttctcgata actaatttct aaaataaagt aacctggaag ttttctatag
    13141 ctgtcaggtg acttcacttc cttgatccga tagacccctt ccaacaagtc tgaagcattg
    13201 aatagggaag aatcgtttgg atctggtgcc aagcctccct cgatgatttc atactctcct
    13261 gcactatttt gtttctcaat tgaaaattct gcgccttcaa gacgtcggct attcatactg
    13321 tcaattttca aaagttccaa atcgattggc gctagttcat cttctaaggt tacttgtagt
    13381 acaccatcga tgatttcata gccttccggc aagggagcag tttcgtcttc gatgaccgta
    13441 tcttcaatga taaacttacc ttcttcattc agtgcaaact ggaacacttg atcagatagc
    13501 cgatacccat ttggtgcgac agattccgag acctcatatt caccaataga cagatgcaga
    13561 ggttggtcgt aatttaaatc gacatacttt tctactccta cggcacgaga atagaagtat
    13621 ccgatcccag gcaaacgttt cgattcttct ggttctaacg gattaccagt gtatgctcga
    13681 tacagcggga aggaagaatc ccccactttc ctgatatcga aacctgcacc atctaatgta
    13741 tggccttcat ggtctaattt tctaactcgg atctcccgat aatcaaacgt attgtaagca
    13801 gtgaatgcta cccataaacg atcctcttca tctgttgtat acgtgagata ttgccaattt
    13861 tcttcacctg tttgcttgag ttggatagtc cctatagcat tatcgtaaag tcgcaagatc
    13921 catgtgtcat tttcatcagg ttctttgaaa ccttctgggt tctggctctc aatgattttg
    13981 tattctgcat agttggtagt ttcttcacta tccttataaa caaatgaatc tggctctccg
    14041 attggatgaa taactttctc accatccacg attacatatt tcccgtcttc atctactgaa
    14101 tagaacgtga gtctcccttt ctcttcctct acttcgttca caccagaaag ccctttcgct
    14161 actggatatc cgatttccag tccgttacca tctgtttcat aaagttcaaa ctctgcgcca
    14221 ttgattggca tattactgcc ttcaattagt ttattgatat tcatgtggat tggcttgaat
    14281 tcatttcgca tttccagttt gacatacaac tcatcatact ctgtgccttc tatgttaggc
    14341 tcttgtacaa cagaaagctt gtgatattct ccttcataat aggaattcgg atctttacct
    14401 tccggtccgc cgactctcga accagatgta tattcatcta caagctcgta gtgacctaac
    14461 atctcatctt cgttggctgc atattttttg atttggaatt gatacgtctc atctactaac
    14521 tgataacgtg gtggtccagc gatctctctg atccaaaata tgtcgctgac tgctccagtt
    14581 tcatctccta aaatcggcat cttcgtttca aagatcgcta ttccgtccgt ggtagtttta
    14641 cttgtttcga ttggttcggc ttccccactc tctggatttt ggtcatataa tgcaaattcg
    14701 actccagcca acggctcgcc attttgaccc ttcttgctga cttggaaatt acggccttta
    14761 tgttcgtaac gaatggaaga agatgggaaa tacatccgtc cagtagtgat atcattctca
    14821 taatcctcca ggttctgaat ataggccata ttgttcactg tgcaaaaatt tccgtcttga
    14881 gctcccgcat caagttttac ttggtacttg atcctgacca tttccccttc acctaaagaa
    14941 atcggagaaa atttataagc ttggttttcc cgtccgacat acgttgtcgg agtcgccact
    15001 acttctggtg gggcttgtcc atgacgttct tcatcccata tttctggctc ttggtacttc
    15061 atgccgtttt ctcctggatc tagatggtaa aactgaatat ctgccgatct gccagctcca
    15121 aatggtgaga cattaaaacc agtttcatca taaagtgtgg cttgctggat cgttctcttg
    15181 aaggtagaag agatatcctc caaggctgta acaatgtcat tcttgttgtt cgcattgtaa
    15241 tatcgcggtg ttacgccatc ttcttctaaa tcagcaatat ttttcaatac atttcgtcca
    15301 atagcatcta gtcgttgtct cccgctaacg tttccatcga ttcctaaacc aatggagaag
    15361 atacttggcg ggttctcttg atacttgttt ttcagccaat gatgataagc aactgtgttg
    15421 atcgcagatg acttttcttg cgaagtagga ctgccattcg tattgttatt agaagaacca
    15481 tcaccaaccc agtgagtcgc tttattatct ccttctttta cctctccata acgatagtgt
    15541 acatttactt catcttctgg acggttaaag tcagatgcat aggtaactgg atgagtaaac
    15601 cctgcattag aagtattccc agaaaactcc tcaaaatttc tgaacaaaac gtcattatct
    15661 tcagcaatct tatcttccat cacagtccag ttgctccaag ctccattagt gctcgtacga
    15721 taacttgact gaatcggtgc ataactgaag gtcggtgtcc catctcccac aacgattagg
    15781 attttttctg gatttctatt ttctccgcca ttgtctttat acaatgtttc atagccattt
    15841 ttaagcccta gtgtaagcgg agttcctccg attggtgtac gatttaatgc tgtattctca
    15901 cttcccgtca aacgattgat atcatttgtc aatggaaaga tgtccgaact gatttgctca
    15961 tggttattga tagctgtact attatggtaa aaattcacca taccgattcg aatatccaga
    16021 ctcgggtcag agagcagatc tttactcatt tcgtttactg cctcaatcaa cgcagcattt
    16081 ttagtttgac tattggtccc agcggttaat tcactcatac tcgctgactt atctagaaca
    16141 aaaacgatat cgattggact gatttccttt ttctcgcccc cgatgacatc tagatagaca
    16201 ttgtaaaggg ttgttgcatc ttcaggatgt agatcagatg ctgtgctgct tggcagaaca
    16261 ggttctaccc attttttgat gtacgccccg ttataatcca agtaactgtt ttcaaaattc
    16321 cgttcatttc cccaaagctg aacggtttga gctggaacgt ttctatcttc ttcgcctgca
    16381 gttgatccat tattagtagc atagtcgtaa tttcgccagt tcgctccccc gtttgtataa
    16441 tcactatcat cgatcaagtt ctctccgtca tatggcgagc ctttgtctcc ttgatagcgt
    16501 tcattgcttt gtgtccaatt tcctaacact tttgtatagt cattcgttgt aaacttcggg
    16561 tagcgatgct gttcgttttc aacttctccg ctaggataga tatattcata ggggtcttgc
    16621 gtagctgttg gcccaagacg ctccaaaata ccgttgaata actggcgttg aatgcgctgg
    16681 agtgcttgac cgctgggtac gattggttcg tccagctttt ccatctcatt ttcttcgcta
    16741 cgttgaattt cagcggaatc ttctgtatca gtttcatttt cttgtaattc ttggttatct
    16801 attctttcat caaccaccac ttgaagaggt agttaccctt cgttcacatc ggggatcttt
    16861 tctgtttcaa aaatcaatgt gccggtttct gcttcttttg aataaacttt tccgacatac
    16921 cattcttgac catcgatcgt ctcgactttt aggtcttctc catcttcatc ataggaggat
    16981 agacctgttc cacgaacgtt ttttacggta ccaatcccgg cggctgccgc atcgattgct
    17041 aaacgtattc taccttcatt ggcactttcc tttttttcaa aagccagatg ccattcaagg
    17101 gtttcttctt tttcaatata cttcccttca atttttgcat agtcctcatc taagaatacc
    17161 tcatcctcct ttgtttctgt tgactcagca gctacatcca gggtcgtcat atgatagact
    17221 gactggaaat atggtccaaa tagcaaactg acagttacta actgcaaaag cttcaatttt
    17281 aaccctttca catccttata ctcctttcaa aaatatttgt cttctttcac aacaatcaac
    17341 aaaacaatgc ccatctaatt ataatttaat ttaattttaa ttttaaatca aatgatatcc
    17401 tttttagtta aaaaacacca attatacaac atttattatt aaaattaaat tataaaattt
    17461 tataacgttc tcatttaaca aaacttaagt tattaagaca aatgaataaa caactacgct
    17521 ttcctcgaaa caaaaaaata aagcacaaat gcgcatttac gcactcatgc tttattcgta
    17581 caattaattc agataacggc agcgcatagg atgccaataa cacttcctaa aatagctcct
    17641 cctaaaatgt cagtaggata atgtaaaagt aaaaaaatcc tcgaataagc gatcaaacaa
    17701 gcaaataaaa aggctaatat tcctgcaact ggacttaatt cgaacaacgc agctactgat
    17761 aaaaaggcta gcgctgtatg tcctgaagga aaagaagaag tcttcggttt acttgtgtaa
    17821 aggacaagcc gacgattttt ttcataaggt cttttccgtc ttgtcgtttt tttgataaaa
    17881 agcaaaccaa caaaaaaata gatcaaactg ccgataagaa tggctagacc atattctcta
    17941 gtatcaggta tcaagactaa aacagcagca aggagcaacc aaataaaacc accattagag
    18001 atattactga taaatttcaa aaaattgatt tttcgactat ccgttttccg ttgcttggcg
    18061 aacttgttga taatactatc atcgatttta gcaatcaaac gcaacatgtt attcctcctt
    18121 tttaagaacc ttttcttgcg tacgatggat aaaaagcagg acttgaaaat aaaacaagaa
    18181 actgaaacct aataaagcaa aatagattgg tcctgcaaac cagaatgcga caagaaggat
    18241 caacgtgcct gccaaataga aaagcgcgct tttgaaatca ttaaaaaaca agaaaaatgc
    18301 agtaatccaa gaagcttctt tttgttccag ttgaagcaag attttgtaac taaagagaag
    18361 cgcgcttaat agaagcaaaa acaacgtagt atacatcaat gtatagccaa ttcccccacc
    18421 ccaatagcgg aaaatctgat aatcgatcaa tagaagaaga aaataaacac ttgctaaact
    18481 atcttgcttt ccataaagag agaagttctc acgaaacttt ttccagaata gtgaaccgag
    18541 ctttttctct tcattttttt tgtaatgata aatcatatac agaactgttc gacttgcagt
    18601 tcctaatgtg aaaacaggca agacgcacag gaaccatagt agctgtaaat ggataagacg
    18661 tggaatccat tcaatgattt ctaagaaata atcattggct tttttaccaa tcgatcgatt
    18721 catcatcctt ttgaacctcc tgtcaaatcc atcccttcaa tgaaataacg ttgtgcaaaa
    18781 aagaacagca aaatcgtcgg gatcaagaca agtgttgctc ctgccatcag ataattccat
    18841 tgtgtcgttg cttggttctg aaaaacttgc aagccaatct gtaacgtgta tttttcttga
    18901 tcttggatat ataacagagg acctaagaaa tcattccaag ctgcgttgaa ggaattgatc
    18961 gcaatggtaa tcaaggcagg tttcgtcaat ggaagcatca aatgactcca aatatacaaa
    19021 tgattcgctc cgtcgatttc agcagcttcg atcaattcat tattgatcga taagtaaaat
    19081 tgacgcatca gaaatatatt gaacgcattg ccgaagaacg atggaacgat caggggcaaa
    19141 taagtaccta cccagccgat cttggagaat aatacatatt gagggatcat cgttacaaac
    19201 cccgggatca tcatcgtaga aagaaccaaa gcaaacatca gctttttccc aggaaaatca
    19261 agcttcgcaa atccataagc aataaaagaa tttgaaagga cattaccgat cactactagc
    19321 acagtgatga ataatgtatt tcttgcatag cgccaaaaag gaaaggcggc aatcgtatcc
    19381 agatagtttt cccatacgat tttttctggg aaaaaggtcg gaggataacg aacgatttct
    19441 tccattggtt tcaatgaagt aaacaccatc caaagcaatg gaatgatcaa aatgattcct
    19501 attactgtca tgaggacata acttagtatt ttttttgttt gccgattcac actggtcttt
    19561 ttgaaataat ccatcatttg ccgtcacctc cagaataata gacccactta ggtgcaagct
    19621 tcaaattgac aaacgtcaga accaacacga tcagaaacag gatcattgac atagccatcg
    19681 cataacccat atcaaaagct acgaacgcct tgttccacat atgcagatta taaaaaagca
    19741 aagaatttgc aggtccgcct gttccatttt tcgtcatgac ataagcttct tgaaaaattt
    19801 ggaaagaacc gatcgtagag gtgatcacat cgtaaaaaat aataggagtg attaatggca
    19861 aagtaatact tttgaacttt ccccaaacgc ctgctccatc gatttctgct gattcatata
    19921 aagatctagg aacattttgt aatgtagcta agtaaagtag cattgctcct ccagaactcc
    19981 atattttcat caaaatcaac gcaggtttcg tccattgtgg atcaaacagc caagctggtc
    20041 cagggatatg gaaccagccc agaaatgtgt tgatcaatcc tgcagatgga gaaagcagct
    20101 gcatccataa gatgtagact gctactcctg ataagactgc cggtaaatag aaagctgtac
    20161 ggaatacact gattcctttg attttttgat tgagtactgt ggctaaaaac acactgaaaa
    20221 tcgtcgtcag cggtacattg aagatgacga aataaacggt gttgtataac gatttccaaa
    20281 ataaatcatc gtgaacgaac attcttttga aatttgctag accaatgaaa ttcatcttgg
    20341 atgtcaaatt ataatcggtg aaactaccaa taactgaaaa aagcataggc cctagcataa
    20401 ataccaagaa cccgataata aaaggcgaga taaataaata ggcccatctt gcttcttttt
    20461 tctttcggat agataaacgc ttcaaatttt cttgcctcct ctgtacctag acagtcaaaa
    20521 atggattatt gattttgctt gactaagttt tctactgctt tttgtgcatc tgccaatcct
    20581 tcttttggtg attttgttcc taaaagaatc tggtcaatct gttcattcac taatgtcgtg
    20641 taatccggag catctaaagg aactggtgtg atcaccgtat ttttaaagtt ttcgtccgcc
    20701 atttgataga tcatttttcc tttatccgtt aggttgtcat tattcactaa attctcattg
    20761 gctgtcttgc tcgccatgat atcaaaactt ttttcgccga atttttcttg tacttctgga
    20821 gaagaaagat atttgatgaa ttcataagag gcctcaggat cttttgctcc gtatggtact
    20881 tccaagacga atccgccacc ccacgaccaa tggcctgaac cttcttcttt ttctgggagc
    20941 tgcatcacgc caaaatcaaa atcatccggt gcattttcca tcaaactaga gtaataattg
    21001 atattttggg cacgcatggc tactaatccg gaaataaatg gatcagatac accagaccca
    21061 aattcggctt ctaatttatt gatcgtattt ttcccataat aatcttgcca gtcttggatc
    21121 cactccaagg cttccacttt atttttagta tcaatcttga ctttttcatc tttgtcaaac
    21181 caactagtcc cgttatctgc gtttagcgcc catacatcag cccctaaatt ccataatgga
    21241 taaaaaccga tgcgtgtaaa ttcttttcct tcttttttat ctagtttacg tgctgtttct
    21301 tctaattcat cccaagttgt cggtagatct tgttcactga ttccagcttc tttaaataac
    21361 tttttattat aaaaaatgac ttgtgtatct gtattgaatg ggatcgcata aggatcacct
    21421 tcatattcta ctgtatccca aagctgcgga taaaaatcct tagaagtgtc tttttctaca
    21481 tatttggaga gattagttgc ttgctgtgct tctgctcttt gggcaacgga atttatgtcc
    21541 tgtacaataa catcgggtgc atttccagct gttattgcag ataaagattt agtccagatg
    21601 tcaccccatg gctgatactt ataatttaca tggatctcgt tttgtgattg attgaaatcc
    21661 tcaatgattt cttcgatagc ttcttttcgt gcaccggatc cccaaaagga ccagaaatca
    21721 atattttttc ttccatcact tgttttttca gaagaatttg ctgcacttga actggcatcg
    21781 cttgcattac ttgatccgca tcctcctaat aaaaacaatg ctgaacttgc cagcaaccct
    21841 gtaaacaata attttttcat attttacctc caaattttaa tgtaaccgat gccggtttta
    21901 atataacgat atcattttgt tctgaaaata acaatttata aggtatgatt ctaaaaaaat
    21961 tattaatgat accaatactt tatataattc gcgagcgctt tctttgaatt ttttaatctt
    22021 tcgtagcaga atgagaatgt attaaaaata gattaggagg aattttgatg tttagacacc
    22081 agaaagaatt gcagtttgaa gcaaaaccag atcgtccgaa tccattaatc gcgaaatatc
    22141 tgcaggagct gatcggcggc caatatggcg agatgagtgt agctatgcag tacctgttcc
    22201 aaggctggag ctgccgagga gaagaaaaat ataaagatat gctgatggac attgcaacag
    22261 aagaaatcgg ccacgtggaa atgttggcga caatggttgc tcgtttatta gagggtgcgc
    22321 ctgttgaaga tcaagaagca gcgatgcaaa aagatccaac aatcggtgcc attatctctg
    22381 gaatgaatcc acagcacgcg atcgtagctg gcttaggacc tcgtcctgct gacagcgtgg
    22441 gcaatccatg gagcggtgcc tatatcattg cgagtggcaa cttgttagca gactttagag
    22501 ctaatttaaa tgccgaatca caaggaagac ttcaagtagc acgtatttac gaaatgatcg
    22561 atgatcgtgg cgtaaaagat atgctgtctg ttctccttgc aagagatagc tatcatcaaa
    22621 atctatggga atgtgcagtg aaagaattag aagaaaaaga aggttcaatc ttagtcccaa
    22681 gtactttccc gcgtgacaaa gaacgaaaag acattgctta tgacttctat aacttctcag
    22741 agggagacca aagtagtgaa ggttcttggg ctagtggcaa agcactggat ggcgaaggcg
    22801 aatatcaatg gttgaaagaa cctaagatcg aaggcgctgc accaaaatta gaaccagtaa
    22861 caccaaaaat gtatgggaca cctccattca agaaataaat atctacccgt agaaacaccc
    22921 gttttttgtt ccacacgctt tgtgagcaaa actgactaaa gtcattaatc ataaccaccc
    22981 ttcaaaaggg tggttttctc tagagctata agctcaaatc accggccagc gcctaaagac
    23041 gctggctttc atattttatg ttcaagccat attgcctttg ccagttttgc ccacctctta
    23101 aaaggtttat gtactactcc ttagccctta aaaggatcgt cttactctcg tacactcagc
    23161 ttatctaacg cactatcatg tttttcttgt tcttgaatgt actttttgat ggtcgcttca
    23221 ttttgtccta cttttgtcac atagtatcct tccacccaaa agtgtcgata cccaaattta
    23281 tattttagat ttgcacaatc tcttgatgat ctcctgaata ttggctcgat attgattata
    23341 aattattttt cttctatact ttggtgtgaa tacaatgtgg tatttacaca tctactttgt
    23401 acgggctaaa ctatttccct ttattgctcc acccgcatag caagtggttt tgtatttcaa
    23461 tcgctcggcg cgctcaatta ggtctaaaga caataaatct aaaaacccgc tgcttcactg
    23521 acagaaacaa cgggttttca gaaaaaaatg aaatgaaaaa gatctcttac ggcaatctat
    23581 tttatttttt tgatttttta taaatggaaa atgcttcaag aagtccaaga aataaagccg
    23641 tataaaaaca agaacctatg aaatctttat ttattagata ttcaacagaa atcccgataa
    23701 cactagccaa caatatattt atcagaagtg taaaataagg atgcttttta tagaatagaa
    23761 ctagtgttgt tttcatagac tctcctttct tcatatttcc aattaatcac aaattcacgg
    23821 cattactaga aaaattaaag gggcgcagaa gtggaaaaat actcttcgac tttccctaca
    23881 aaccaggcca tcttgtgttt aataactata ccaaatctaa ggtacctcta aatctaaatt
    23941 ccatttgtgg cggtgacctc caatttttaa ctcttctatc tcccatccag tatacataac
    24001 cttgatactt cgaccactta tatatcgaac acacaaatac ctatccattg caggagtact
    24061 acctaactta gttgctactt gcatctaaat aacagctgaa aagataggac ttactcttag
    24121 taaagtatct gaactattaa tttttaaact atcattttta tcctcaacta catgcacatc
    24181 tgttgcaact tcactttctg tctcatccag ataaatgatt aaggaatcgg gaattgcaat
    24241 atcttcagct gacgctggaa tagttgagct aaattagtca tcatatcttt attataaaat
    24301 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
    24361 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
    24421 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
    24481 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
    24541 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
    24601 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
    24661 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
    24721 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
    24781 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
    24841 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
    24901 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
    24961 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
    25021 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
    25081 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
    25141 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
    25201 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
    25261 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
    25321 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
    25381 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
    25441 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
    25501 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
    25561 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
    25621 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
    25681 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
    25741 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
    25801 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
    25861 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
    25921 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
    25981 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
    26041 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
    26101 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
    26161 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
    26221 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
    26281 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
    26341 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
    26401 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
    26461 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
    26521 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
    26581 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
    26641 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
    26701 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
    26761 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
    26821 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
    26881 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
    26941 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
    27001 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
    27061 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
    27121 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
    27181 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
    27241 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
    27301 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
    27361 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
    27421 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
    27481 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
    27541 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
    27601 ta
//
LOCUS       DAZKSU010000036        26931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000036 DAZKSU010000000
VERSION     DAZKSU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.036"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..1145)
                     /locus_tag="ACG8Z3_001548"
     CDS             complement(39..1145)
                     /locus_tag="ACG8Z3_001548"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0823864.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1355..2356
                     /gene="ccpA"
                     /locus_tag="ACG8Z3_001549"
     CDS             1355..2356
                     /gene="ccpA"
                     /locus_tag="ACG8Z3_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0823865.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2693..5038)
                     /locus_tag="ACG8Z3_001550"
     CDS             complement(2693..5038)
                     /locus_tag="ACG8Z3_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0823866.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5317..6219
                     /locus_tag="ACG8Z3_001551"
     CDS             5317..6219
                     /locus_tag="ACG8Z3_001551"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0823867.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            6432..8207
                     /locus_tag="ACG8Z3_001552"
     CDS             6432..8207
                     /locus_tag="ACG8Z3_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0823868.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8273..9859)
                     /locus_tag="ACG8Z3_001553"
     CDS             complement(8273..9859)
                     /locus_tag="ACG8Z3_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0823869.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            10110..10334
                     /locus_tag="ACG8Z3_001554"
     CDS             10110..10334
                     /locus_tag="ACG8Z3_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0823870.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10476..12227
                     /locus_tag="ACG8Z3_001555"
     CDS             10476..12227
                     /locus_tag="ACG8Z3_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0823871.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12227..14011
                     /locus_tag="ACG8Z3_001556"
     CDS             12227..14011
                     /locus_tag="ACG8Z3_001556"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0823872.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14337..15188
                     /gene="licT"
                     /locus_tag="ACG8Z3_001557"
                     /pseudo
     CDS             14337..15188
                     /gene="licT"
                     /locus_tag="ACG8Z3_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            15281..17167
                     /locus_tag="ACG8Z3_001558"
                     /pseudo
     CDS             15281..17167
                     /locus_tag="ACG8Z3_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(17207..18109)
                     /locus_tag="ACG8Z3_001559"
     CDS             complement(17207..18109)
                     /locus_tag="ACG8Z3_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0823873.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18340..19743
                     /locus_tag="ACG8Z3_001560"
     CDS             18340..19743
                     /locus_tag="ACG8Z3_001560"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0823874.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19743..21176
                     /locus_tag="ACG8Z3_001561"
     CDS             19743..21176
                     /locus_tag="ACG8Z3_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0823875.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            21321..22247
                     /gene="trxB"
                     /locus_tag="ACG8Z3_001562"
     CDS             21321..22247
                     /gene="trxB"
                     /locus_tag="ACG8Z3_001562"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0823876.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22461..24188
                     /locus_tag="ACG8Z3_001563"
     CDS             22461..24188
                     /locus_tag="ACG8Z3_001563"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0823877.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24203..24553
                     /locus_tag="ACG8Z3_001564"
     CDS             24203..24553
                     /locus_tag="ACG8Z3_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0823878.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(24645..25505)
                     /locus_tag="ACG8Z3_001565"
     CDS             complement(24645..25505)
                     /locus_tag="ACG8Z3_001565"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0823879.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            25597..26025
                     /locus_tag="ACG8Z3_001566"
     CDS             25597..26025
                     /locus_tag="ACG8Z3_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0823880.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            26187..26363
                     /gene="rpsU"
                     /locus_tag="ACG8Z3_001567"
     CDS             26187..26363
                     /gene="rpsU"
                     /locus_tag="ACG8Z3_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0823881.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            26390..26836
                     /locus_tag="ACG8Z3_001568"
     CDS             26390..26836
                     /locus_tag="ACG8Z3_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0823882.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         8570 a         4797 c         5691 g         7873 t
ORIGIN      
        1 aaaaggtggg agcggactag ctctcacctt ttttactctt atttttctaa aacaagtaat
       61 tcttttggtg tagtcgtgaa tggcacacag ccatcgcttg tcacatatac acagtcttcg
      121 atacgtaccc ctactttttc aggaatatag attcctggtt caagagagaa gcacatccct
      181 tcttcgatta ctaagtcatt tccttctaca agggaaggga attcgtggac ggttgttccg
      241 atcccgtgac ctaaacggtg attgaaatat tcgccgtagc catagctgtt gatcacgttt
      301 cgtgcgattt gatcaagttc acctgctgtc acacctggac ggacggcttc ttgtgcggca
      361 agctgtgctt caagagtgat gttgtagatt ttttcttgga agtcggttgg tttttgataa
      421 gcaattgtac gagtagcatc actgcaatag ccattccaaa caacgccaag atcgaataac
      481 acaagttcat tcggtgaaat cttcgtattt cctggtgtac catgagggct tgcagcattt
      541 gctcctgcaa ggactaatgt gtcgaaagac atttgtgaaa caccttgttt tttcaattgg
      601 tattcaattt ctgcaatgat cgcttgttca gtcacgcctt ctttcactgc tttaaagcca
      661 atctcaaatg caacatcggc ccatttacca gcttcgatca gtttgtcttt ttcctctaga
      721 gttttgatca gttgtagacg ttggatgatg gaagttagat cagtgtaaga atccgttccc
      781 gtcaagatat cagagatagc atcgaagcgc tcgacagtca aggcgttctt ttcaatccct
      841 acattgccag gctgaccata gcgagcacga agttcttttc cgatgatctc ccaagggttt
      901 tcgctgtcta aatagccacg gacatcatac gcccaactac tgttttgagc atcttcaact
      961 tccaaagcag gagtgaaaag gaaaggatct tgttcaagag ggataaaaag agctagtaca
     1021 cgctcatgag gatcactttc gtatccggaa aagtaagcga tgtggccagg gtcgctgata
     1081 taagccaatt cgacttggtg ttgtgccatc cattgacgta gttcattgat ttttacgtta
     1141 ttcatttggc aacctacttt ctaaattaat tttgtattca ataattgtac cactgattta
     1201 ttaaaagacc aacgttctaa aaactgaaaa aagtaaaaat tttcatgaaa acagagataa
     1261 aacggttgca attcttgaga ttatttgata ttctatttta gaaatgtaag acgtattttc
     1321 agaaaacata taaaatatga ataggagaac acaaatggaa aagcaaacaa tcactatcta
     1381 tgatgtagcg agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa
     1441 tccaaacgtt aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc gcttggatta
     1501 tcgtccaaac gcagttgcgc gtggcttggc aagtaagaaa acaacaacag ttggtgtaat
     1561 catccctgat gtcagcaata tgttctttgc ttctttagca cgtggtatcg atgatgtggc
     1621 aacaatgtac aaatacaata ttatcttagc aaattcagat ggcaacgacc aaaaagaagt
     1681 caatgtattg aataacttac tggctaagca agtggacggc gtgatcttca tgggtcacca
     1741 tatcacagac gaaatccgtg gagaattttc acgttcaaaa actcctgttg tattagctgg
     1801 ctctattgat ccagatgaac aagtaggtag cgtaaacatt gattatgtat ccgcaacaaa
     1861 agatgcagta aacaaattgg caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc
     1921 gttgatcgat cccatcaatg gacaaaatcg tctgaaaggc tataaagaag cattaagcga
     1981 aaataaccta ccatatagcg aaggattgat ctttgaagca caatacaact tcaaagatgg
     2041 tctatctcta gtcgatcgga tccataatag cggtgcaaca gctgtctatg tttcagatga
     2101 tgaattagcc atcggtattt tggatggttt attagaccgt ggcgtgaaag taccggaaga
     2161 attcgaaatc atcacaagta acaattcact actgacagaa gtagctcgtc ctcgtttaac
     2221 aagtatcaca caaccattat acgatttagg ggctgtatct atgcgcctat tgacgaaaat
     2281 gatgaataaa gaagaagtcg aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa
     2341 aggatctaca aaataataaa aggacaacca ttcagaggtt gtgtgcctgc cgtttaactc
     2401 tgcgttacaa ggagcaattt caaaaaacag catctcatgt ttgatgaata ttgtgactcg
     2461 tgattcggag ttggcaggag aacaccgttt atttgggaaa aggggttatg acaaaatttt
     2521 gttataatcc tttttttgtg tcttaaaaaa gagagaaagt ggttgaaatt acatttatcg
     2581 tatataaaac aatccgtgta tagatgctgc attttgttca ccaccatcaa aaaaataagg
     2641 gactgtgaca atactattgt cacaatccct tattactgtg tattctatta aatcaatcat
     2701 ctttctcatc tgattcttta gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc
     2761 agtaatctgt atagttttca tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg
     2821 gtccattttt cgcccatagc gattcaactg tttttcctgg tgtttggatg gattttttat
     2881 tgacagtaac ttttccaggt gtttgaccag taaatccaga tacttttttc ttcatcacat
     2941 cagatgataa ttcgaatttc tcatcagtgc caaaaatgtc aggttttgct tggtaagctt
     3001 gattgatcag attggcaaga taattagccg aacgtatcct tgctttgctg tccattccag
     3061 tattgtcatc gtgccctgcc cagctactga tggtaataga cggagtagag acaattagcc
     3121 atgaatctcg gtattcattg gtagaaccag tttttccgac ccaatctgct tttcctagat
     3181 ttccgtttaa gcttgagatg gcatctttga atggtgtagt gatttttgca ttgatcacac
     3241 tacgcatcat gtcattcatg atcgatgccg ttgcttccga atagatccgg acagagttgc
     3301 tttcgtgttt ataaattaca tttcctgcgt tatctgtgat ggaatcgatg atatagcctt
     3361 cttcatagac gccgccgttt gctaaagcct gaaatccgtt ggtctgttga agcgtcgtga
     3421 catctactgt acctaaagga gcagattcaa cgccccaatt gtcatttgcc gggtagttca
     3481 tctttgccaa gtagtgctcg taagcatatt gtttcgatcc accggaattc aacacatctt
     3541 gatataagtg ataagccggg atattgttgg accattctag tgactcacgg acggtttgga
     3601 aggtgttcga tccttcgtta gtagcattga cgattttctc gccagcattt tctccttctt
     3661 gccaagttgt tgcataatct gatacacggg attctgatcc gataagcccc atgtcgattg
     3721 caggtccata aacaagaact ggtttgattg atgagccagc ttgacgttct gtatcgaagg
     3781 catggttatt ttgattctga ctgtaattcc gtccgccaac gaatccgtag atgcggccag
     3841 ttctgttatc catcaataca tttccggttt ctacttgact gccagcacca tcatctaaca
     3901 tgtatccata attttcaacg ccattttgca atgcagcata gacatcttta tcgattgtcg
     3961 aatggattgt atatccttta ttttggatcg tttgctgtgc cttttgataa taagcgtcac
     4021 tgaccgatga atcagacata tcagcgttgt ctttttcagc caattgttgt gcaatgattc
     4081 ttgttgcttc attcatcaca gtataataca agaattctct gtcagtctgt tctgcgattt
     4141 gttgtggtag aaagtctttt gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg
     4201 taatttggcc ttcacgatac atactgaata aaacaaaatc ttttctggca aggccggcag
     4261 aaagatcttc ttttagcgcg cctgtgttcg tgtatggact atacgtgatc gggctttggg
     4321 gtaatccagc gatataagca gcttgcggca gcgtcacatc ttttgcatct acaccaaaga
     4381 ttccttttgc agcttcctgg actccagcaa tgttttgtcc cttattatta cgaccaaatg
     4441 gtgagacatt caaatacgta gcaatgatct catcttttga aaagtatttt tctacttgtg
     4501 cagaaagaat gatctcgttt gctttacgtt tgaatgttgt ttcatctgta aggatctgct
     4561 gtttgactaa ttgctgtgtc aaagttgatc caccagaaga gccaatacca gtagcttcag
     4621 atactaatgc tcgtaaaaca gctttaggaa cgtaaccttg gtgtttatcg aagtattcat
     4681 cctctgtgct gataattgca gttttcaata aaggtgaaat cttatcagat gaaatagtcg
     4741 tgcgcatcag atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg
     4801 acgtttctgt aatgtttcct agttctgtct gcagtgcttt tttagtcggc aattgtgtgt
     4861 cttcgacgag ataggcaaaa tagccagcgc ctattccaat acctagagct gcgaacatca
     4921 aaagaagaac agtaataaaa acagtcaatg attgaaaaac tctaaaaata atcttaggaa
     4981 caaaccattt ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa
     5041 gggtcacctc aactaatggt tttatttcgc taattatagc aaataataga acaaaaaaac
     5101 agacaaggct gtttttggca ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga
     5161 tctttattat aaaagagttc ttaagctttt ctttagagaa aatttcagtt actatggaga
     5221 acagggcaaa gaaaaatcaa tcattttctt tgtatatcat cttaccaata tataggaatt
     5281 gttagtaaca attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc
     5341 atgagcggaa agaaaatgct gttaggagca gcagaagaag cagccagtta tggatcttcg
     5401 acttttatga tctatacagg cgcccctcaa aacactagaa gaaaagccat cgaagagatg
     5461 aatattgatg ccggaaaagc gtacatggca gaaaaaggtc taacaaatat cgtcgttcat
     5521 gccccttata tcattaattt ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc
     5581 caatttctga gagaagagat tgttcgtgcg gaagctttag gcgctactca aattacactt
     5641 cacccaggtg ctcatgtagg ggcaggaaca gaagccggaa ttgctagaat tattgaaggc
     5701 ttgaatgaag ttttgacaaa agaacagacg gcacaaatcg ctttggagac aatggcaggt
     5761 aaaggtacgg aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa
     5821 ttgaatgaaa aattatccgt tacttttgac acctgtcata cgaatgatgc cggttataat
     5881 attaaagaag acttttcagg tgtaatggaa gagtttgata agatcatcgg gttagatcgg
     5941 cttaaagtac tccatatcaa tgattctaaa aatccgcaag gttcgcataa agaccggcac
     6001 gccaatattg gttttggaac aatcggattc gacacgctga ataagatcgt ccatgatcca
     6061 atgtttgaca atatttcgaa aatcttggaa actccctatg taggcaaaga taaaaagaaa
     6121 gcttacgcac catatggaaa agaaatcgcc atgttcaaag cgcaaacatt cgatccagaa
     6181 gtgtttcctg agttattagc agaagaagtg atactctaag attttctatc tgacagcagc
     6241 aaaaaaggac caggcacatt gccttgtcct tttttgttta agaaacaaag gagtactttt
     6301 tgcagtcttt ttcatcatgc atcttctccc ccctcttttt aaatttagac aaacaaggta
     6361 tactggtaga aaagtaaaaa aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga
     6421 aggacgaaaa gatgctgaac actttaaaac ccttgatcaa ccaactaaaa aagaaacata
     6481 catactttat tcttggcata gccctattat ttacaattat tcgtttgtat ttatatagta
     6541 aagcaatcta tggaacagac atgtcggcaa cctatgatga ccagctgctg attcgctatg
     6601 ctcaaaatat tttggacggt aaatggctag gggagtatac gactacaaca ctttccaaag
     6661 gaatctccta cagcttattt ttagtacttg cacataaact gcatctttct tatgcagttt
     6721 tgttcggcat gttcagtatt tttgccagta tattggttgc actcgcgttt aaaccgattg
     6781 tgaagaacaa gtatcttctt ttaagtatct atttttattt tttgttttct ccgattagtt
     6841 ttacacatga atattccact cgaacatatc gtaatgccct cgtgattccg gctgttgaga
     6901 tcattatcgc ttgtttatta gctatttatt accgcaaata cggttctagc agaaaattgc
     6961 tgccatggtt tttagggctt tctgtcgctt ttcctttctt ttggttcatt cgcgaagatt
     7021 ctatctggct tcttcctttt actgtagtag ctttgggtat cagtttcgtc cagatcttgc
     7081 tagggcaaga cttttctttc aaagaagtaa agaagttaaa aaaacgactc tttcttctct
     7141 cgaaacggga atggctgaaa ctttcattgt ttttacttcc tatagttggc ttttccatcg
     7201 tctatcaatg gatcgaatat aaaaatgaga caacatacgg tattgcaacc atcaatgacc
     7261 gttccagtgg agaatttgga cgtttgatga aacaactgat ccgaatagat gatgaaacga
     7321 acttgaataa agaaaacagt gagatatggg tctcacatgc tgcactaaat aaagcattag
     7381 ccgcatctcc tacattccat actgtttcta cccgtattga cgaattatat acgactcacg
     7441 cgtggactca aggtggaaaa gtaaaagaat tacccggaga tatcatcttt tgggcattgc
     7501 gagatgtggt aagcgaatct ggttattacc aaaacaatgc aaaagaaacc aatgattttt
     7561 ggaaaaaagt caaccaagaa ttagaagaag cctatcacaa gggaacaatc aaaaagaaaa
     7621 aagagttcta tctgatggga tcaggagatg gaaagacgat gagtgatctg ccaatcgtat
     7681 tcgatttcct tcaatcagga tacgcttatg catggcgata taaagatttc catcaaggag
     7741 gaaattatag tttcggaacg caagaacaag tcaaacaggc tcaagaatta ttaaaccttc
     7801 cgctaatgga gaattggatc gatagtaatg aaccgagaac acctaatttc caattaacaa
     7861 aatcagcaaa agtcgcgaat gtcatcataa aaatttacca agttctggct attcccgtgc
     7921 tgattgcttc aatagcaggg atattactta tgatactagg aaccgtattt gcaaaagctg
     7981 atcgtgccta ttatagttcc tctttgattg tggtcaccgg attaatactg acacaattgg
     8041 tttttctttt cggtgtctca tggttttgtt cttatgcgcc agaaaaaaag gactattttt
     8101 taagtgtcta cactggtgca ggcgtgccat taacgcaatt catcgtagta acaagcatgc
     8161 tagttttcag taaattaaat ctgaaaaaag ctttatcttc taaataaaaa gacccttggt
     8221 agctagattt ttaacctaac tatcaagggt ctttgtgtgg aaaaatcagt ttttattcga
     8281 tttcttctac cgtttcctgc aaaagctgtt catatttttt gtttccttta tataaatcac
     8341 caatcgtcca tgccaacagg agaaggacaa gaacaatgat tacataagcg atgtgatcag
     8401 ccaatgctag ttgagcggaa gtggcttgct cgccgaagaa actttcgatc tgtcctggta
     8461 atcctcgtaa gttcaagtaa gttagactaa tcacagagaa ccagccaaga agttttacga
     8521 tgaatgaatt tttaaatcgg ctacccatct ccgcttggct gttggtcatc atcagtaaag
     8581 gaatcatcga aaacggcaac gcaaaagcca agaacacctg ggattcattc attaagttat
     8641 tcaaagcggc atgttcttcg atcgttcctt ttgtacttgt aaacaaaaca caaatcaata
     8701 caggaatcac agaaagcaat cgtgtgacca gtcttcttag ccaaatcggg atacgcatat
     8761 ggatgaatcc ttccatgatg acttgaccgg tcaacgtacc agtaatagta gagttctggc
     8821 cagaagcaag aagtgctaca gcaaataaag tggacaagat accagatttt gctacagcaa
     8881 tcaaaatacc attgctcata gctgatggat cagataaagc ttcatacaga ccaaaaaagg
     8941 aaggatcttg gacagaaccg gttttgaaga cagcgacacc catgatcaat agcagtgcat
     9001 tgactagaaa agccattgac aactggatat tagaatccca agtagaaaaa cgtacagctt
     9061 gtgctacatc ttcttcgttt tggtgatcga tcttccgagt ttgtgaaact gctgaatgaa
     9121 gatataagtt atgcggcatg actgttgcac caataatccc taaagctcca gtcagcggag
     9181 tttcgccgcc gatgaccggt gagtctgaga atgtatgact gttagggata aagccagcaa
     9241 ataaactgtg ccaatctggg ttagacaagc caacttgata gacaaaaacg aataaaatga
     9301 cgaaaattaa gcagacaacc agtgcttcga tcttacgaaa cccgactttt gtcagtagta
     9361 agagaacgaa aacatctaat acagtaataa agacggcaat caccaatgga atatcaaaaa
     9421 gtaagtagag ggcgattgct gcaccaatga cttctgcaat atctgttgcc ataatggcta
     9481 attcagtcaa gatccataaa atgacaccta atgtcttact tgtgcgtgct cggattgcct
     9541 gtgccaaatc catttggctc acgattccta attttgctgc catatactgt aaaagcatag
     9601 caatcagact agacatcaaa ataactgaca tcaataaata ttggaaattt tgtcctccgg
     9661 tgatcgaagt agaccagtta cccgggtcca tatacccaac tgctactaac gcaccaggtc
     9721 cagagtaagc aagaagtgtt ttaaaaaaac ttttcccctt tggtacatca actgttccat
     9781 ttacttcagc taaagataaa ccatttgtat gttcaataag tcgatgctta tgaaattgtt
     9841 tattggattg attattcaag aaaaaaccac ctttcctatt tatctacgat tacattctac
     9901 acctcaaaaa gaaaaaatta aagaaaaaga ttcggtacgc ctaatttttt ttcatggcgc
     9961 gtcttgctag atgacgaaag aaattttgtt ctcctctaaa aaaaccacta ttttttgcat
    10021 gaaactaaag aatacgcctt tcttttttca ataaacttta gtaaaataag tcattgagag
    10081 caattcggaa atagaaagga gagatttata tgttatcaac aggtattgtc gtacttattg
    10141 ctattattgc tttgctaatc ggcgcagtag gtggcttttt cctagcgcgt aaatatatga
    10201 aagactattt agaaaaaaat cctcccgtta atgaggacat gttgcgatca atgatgatgt
    10261 caatggggca aaaaccatct gagaagaaaa ttcgccagat gatgcagcaa atgaagaacc
    10321 aagggaaaaa ataggttctg acccaacgac tttttacggg ctgatttttt cagtccgttt
    10381 tttatttcgt aatttgttag actattcaga ttattcagca aaaaagacaa gcacagacaa
    10441 tcctaggctc ttgataaaga caaaggagga gtaaaatgtc gatttttaga aaattaggct
    10501 ggttttttcg tcaggaaaag aaacattatt taattggggt tttctcgcta ttcgcagtgg
    10561 ccttggtaca gttagttcct ccaaaagtga ttgggatcat cattgatgag atcgccgata
    10621 aaaatttatc aataaagatc attttcgggt ggatcttcct attgattatc gctgcttttt
    10681 tacaatatct ttttcgttat atctggcgga cgaatatttg gggaagtgca gctcgattgg
    10741 agaaagaact tcggcagcag ttattcaaac attttacaga aatggacaat ctcttttacc
    10801 aaaaatatcg gactggggat ttgatggctc acgcaacgaa tgatttgaat gcgatccaga
    10861 atgtagcagg agctggtatc ttgacctttg cagattcagt gattacgggc ggaacaacga
    10921 tcattgcgat gattttgttt attgattggc gcttaacact gattgcgctg attcctctgc
    10981 ctttgctggc cgtcacttca cgagtgctgg ggtcaaaact ccatgatgct tttcgtgatt
    11041 cgcaagaagc tttttctagt atcaatgata agacacaaga aagtatcact gggatcaaag
    11101 tcattaaaac atttggacaa gaaaaagaag atctagcgga ttttgaagaa aagattgaag
    11161 atgcaattat taaaaacaaa cgagtcaatt ttttagactc cttatttgat ccattcatta
    11221 ccttgattat cggtctgtct tatgttgcaa caattatttt aggtggaaag tatgtcttgg
    11281 aaggcacgat ctcgatcggg cagatggtct catttattag ttatatcggt atgcttgttt
    11341 ggccgatgtt tgcgattggt cgattgttca acgtattgga aagaggaaat gcttcttatg
    11401 atcgcgtcaa tgaacttttg catgaaaaga cgcatatcgt ggaacaactg ggtgcagtgc
    11461 agaccccggc aaaaggcgag ctgaatgtag ctatttcctc ttttaattat ccgaaagaag
    11521 aatcaccagc attagaacaa atcaagtttg cgatccaaga aggagaaaca ttaggtattg
    11581 ttgggaaaac tggtgcggga aaaacgacga tcatcaaatt actgatgcgt gaatacgatg
    11641 attatcaagg gaaaatccaa tttggcggac gttcgatcaa agaatattca ttgaatgctt
    11701 tattaggatc aatcggctat gtgcctcagg atcacttctt gttttcgatg accattcgag
    11761 ataatatccg tttttctaat ccaaaacttt cagaagaaga agtagagcat gcggcttctt
    11821 tagcatttat caacaatgaa atcaaggaat taccaaaagg ttatgatacc ttagttggag
    11881 aacgtggagt ctctttatct ggcggacaga aacaaaggat ttcgattgct cgagcattga
    11941 tcaaaaatcc tgaattatta atattagatg atgcactctc tgcagtagat gccaaaacag
    12001 aagaagctat cttgcataat ttaaaagaaa taagaaaaga gaaaacaact atcattgcag
    12061 ctcaccgttt aagtagcgtg atgcatgcaa aagaaatcat tgttgtcgat caagggaaaa
    12121 tcattgaacg aggaacccat catgagctgc ttgcactagg cggctggtat gaacaaatgt
    12181 gggagaaaca gcaattagaa gcgaagatag aaggaggcga ggcctgatgg atgaaaatta
    12241 tcaatcggaa tggacaaaat cgattccact taaagaacag aaaaagatca tcaaacgact
    12301 gatgaagttc gccaaacctt ttagaagaac gttcatcgtt gctatcttat ttgcgtttgc
    12361 tttgtctgtg atcaatgtcc tattgccaag gatcatccaa acttttatgg atgatcatct
    12421 ggctaaacag tcggctacga cacaagttat tttgtttttt gctggtatgt acttatttgg
    12481 agtcatagta aaaagtatca tttggttttt ccaatggtat ttgtattcca tggcctcatt
    12541 gaagacctat cagtatatta gggtaaaatt atttgaaaag ctgcatacac taggtatgcg
    12601 ttattttgat cagacaccgg caggatcaac cgtttctcga gtgacgaatg acacagaaac
    12661 gctatttgaa ttttggtacg tctttttgat ggtattgact gggatctttg cagtcgtttc
    12721 ttcattcctt gccatgtttc aaatcaatgg agaaattgcc ttgtggactt tggtgttttt
    12781 gccgatttta ggaatcgtca tctggtatta ccagaaattc agttctcgta tttaccgaaa
    12841 tatgcgggaa aaattaagtc agctgaacac gaaattgaat gaatcgattt ccggcatgcg
    12901 tattatccag cagtttcgcc aagaagctcg tttagctaaa gaatttgaac aagtgaacaa
    12961 tgagtattta gacacgcgtt atgcgatgat caaaacgaat tcgattctgt taagtccgat
    13021 catcaatttt ctatatgcat tggcgattgc gttggtgttg actttgtttg ggatcgatgc
    13081 gttgcattct cccgtagaag ttggactgat ctatgcattt acgacctatg tccagggatt
    13141 ttttaatcca atgtctcaaa tgatggactt tttgagcatc ttcacagatg ggatggttgc
    13201 tggtagtcgt atcttgaaga ttatggatac ggaagaatat gcaccacaac aaaaccctgg
    13261 agcagatcaa atgattactt caggtaagat tgagtttcgc aatgtaagct tttcttatga
    13321 tggagagcac aatgtattaa acaatatctc ttttgttgct aatccgggag agaccgttgc
    13381 attagtggga cacaccggaa gcggaaaaag ctcgatcatc aacgtattga tgcggtttta
    13441 cgaattttat gaaggtcaga tattgattga tgatcatgac attcgagaat atccaatgga
    13501 agagttacgt aagaaaatgg ggctggtctt gcaagatgct ttcttgtttt atggggatat
    13561 cgcaggaaat attcggatga tgaatccagc tatcacagac gaacagatac gccatgcggc
    13621 tgagtttgtt caagcaaaca aattcatcga tgaattacca aaaggatatc actcaaaagt
    13681 gatcgaacat ggtgccagct attctagtgg acaacgacag ctgatcactt ttgcgcgaac
    13741 aatcgtgact gatcctaaaa ttctagtatt ggatgaagca acagcaaata tcgatactga
    13801 aacagaagga ttgatccaag aaggattggc aaaaatgcgt caaggacgga caacgattgc
    13861 gattgcccat cggctgtcaa cgattcgaga tgcagaattg attttagttt tagacaaagg
    13921 tcatatagta gaacgcggga atcatgaaac acttctagcg caaaatggtt tgtatgcaga
    13981 tatgtacaaa ctgcaaagca gtggtatata aaagatgttg tgacagtaga attttgacct
    14041 aagcactaga gtgaaaataa aacatagctt ctcatatttt tcatctgtga gagttagtgt
    14101 cgtagtcacc agatttaaaa aaagattcga cagctagtca gaagtgccta atagccacta
    14161 agaaagcgtt gacaaaagca tttgctttcg ataatataaa gaaaacgata caacttggat
    14221 agtgattgcc tgatgcaagc taaacctgat gagtctccca tggtttcatg gacggctttt
    14281 tgggtttttc tttttgtttt ggatacagcc ctatacatca tggaaaggag caaaacatgc
    14341 tgattgagaa agtactgaat aacaacgtcg taataaccag ggatgaaaga aatcaggaaa
    14401 tgatcgtgat gggaaaaggt ttggcattca aaaagaaagt gggtgacccg atttttgact
    14461 catgcatcga caaaaaattt cagttgacaa atgacgggct ttcacaacag ttctaagaat
    14521 tggcacaagc gattcctctt gagtacttag aaatttcttg tgaaatcatt gaatatacaa
    14581 aagaggctct acaagtccag ttgaatgatt ctgtgtatat tgcattgacg gatcatttgt
    14641 atacggctat cgaacgtcac aaaatgggaa tatttgtacc aaatgtattg ctgtatgata
    14701 tcaaacaact atttcctaaa gagtatgcca tcgggaaaaa aacagtgaag aaaatctcgg
    14761 ataaatacca catcaagtta tcagaagatg aagccggatt tatcgcattg catttagtca
    14821 atgcacaagg cattcaaagc cagcaagaca atacctacct gtttactata acaatacagg
    14881 atattctaaa tatcgtccgc tattactata agattacatt tgatgaagat tcagtccatt
    14941 tttttcgatt cgtcacacac ttacgttttt ttctgacacg cgtcctagat ggagaaaaat
    15001 cagaagtggg agaagtttct gaggaattgt ttcaggttat tcaaaaaaaa tataaggata
    15061 cgtttggttg cgttgagaaa atagcagact ttttagccga gacgttcaac taccagatgt
    15121 ccacggacga aaaaatgtat ttaacgatcc atactgcgcg tttagtgcat gagaatcgtt
    15181 gtaaataaaa aaaggaggat aaattaacaa gaacgtatga aataagggta gtgacattca
    15241 gttggctaaa ccttccacta ataaattgga gggaatagaa atgggaaaat accaagaatt
    15301 agcgagaaaa atcgtagaaa atgtcggcgg gaaggaaaat atcaatggtt tgacacattg
    15361 tatcacaagg cttcgtttca aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa
    15421 aaatatggac ggtattgtaa cggtaatgcg agcgggaggg cagtatcaag ttgtcattgg
    15481 gaatcatgtg ccagtcgtgt ttgaagaggt tataaaagca ggaaacctta cttttgatga
    15541 ggctgtttca acagaaaaaa tgagaccgtt tgatatgctt attgatggaa tatcgggctg
    15601 tttccagcca ttccttgcta tcctagctgc tggcgggatg attcgtggac tgacagcatt
    15661 ccttgtcttc ttaggcgcgt ttgaccgcgg ttctggcaca tttgttatgt ttgataatat
    15721 cggtgattct gtattccaat ttatgcctgt tattatcggt ttaacagctg cccgtaaatt
    15781 taaagtaaat gaattcgtag ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc
    15841 gctggaagct ttgagtggtg cagcagaagc accactaacg accatatttt ctggcactat
    15901 ttttgaagca cctatttatc aaacagtatt cggcattccc tggattgcgc gtaactacgc
    15961 ttcttctgtc attcccatta tctttattgt gttgttagct agccaaattc agaaaccaat
    16021 taaaaagtta gtacctgaga tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat
    16081 tacaatgcct ttaggttttt tactcgttgg accagtattc acattcgcaa ctgatatttt
    16141 aatggctgga tttgaaacac ttttagcact ttcgccagtt atttatggag caatcgtagg
    16201 cttcttctgg caaattctgg tgatgtttgg tctccattgg gcaatcgtac caatgggatt
    16261 gatgcaattt tctgtgaatg gttggcaaaa tattatgaca cctgttgcag tagtgtcttt
    16321 tggacaaact gcagcactta ccgcactcta ctttaagctt agaaatccaa aagacaaagc
    16381 aattgcaata ccagccattg tttctggtat tgtggggatt acagagccag ctatttatgg
    16441 gtttacatta ccacgtaaaa aaatatttat ttttacctgt gtcggtggtg cgttaggggg
    16501 cgcgtatagt gggttaatga atttaacttc ttggaatcaa ggtggactag gcatctttac
    16561 aattcccaac tatattagac cagatggtga tttaacagat gtgattaatg tattgattgg
    16621 gatcgccatt gcgatggtgg tatcctttac gctgactttt ttcttttgga aggatgaagc
    16681 aggtgaaaca gatgatatac agaaaaaatc aggtaaagaa attgtaaaaa caccgataca
    16741 aggacaaatc gctccactaa atgcagcaaa ggatgcagct tttgcacaag gaacactagg
    16801 tcgaggaatc ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt
    16861 catgaccctg ttcccgacga aacaggcaat tggaatggtt tctgaaacag ggctggaatt
    16921 gctgattcat gtcggattgg atactgtgca gttggaaggg aaatattttg aaagcttagt
    16981 gcagcaagga taaagagtga aacaaggcga tctgttgcta cgcttcgaca aaaagaaaat
    17041 cgaaaaggaa ggatacagtt tagaaacacc tgtgatcgtc accaattaca cggattattt
    17101 ggatattttg gaaaaccaag gggagacggt cgggccagac gatgcactta tcaccgcact
    17161 gacctgaacg gttaggtgaa gaatggacta ggcttaacta ttctttttat tccacttctt
    17221 tattttcaaa attgaaaggc tctttcatga tggctgagct gatagggcgt ttatcgaatt
    17281 tttgaccgac agaaattaga tggactaaat tctgttgata attttctgca aaaacacaac
    17341 tatataacac gtctagaaga taacaaattg atgtattgat cgtaaaatta ccaatttttg
    17401 aatacagtcg ctcgcgtgtc gtaagtcgca aagcggctgt actcattgca gatagcgttg
    17461 aatcaccgat actggtcaaa gctaaaattg gaacatgatt ttgtttcaat atttctgcgg
    17521 tacgaaggat cacattgttt tcacccgtat aagaaatcag aatcccgcac atatcactcg
    17581 tgcaattgta cgcttcatag aatgattcac caaaagtggt gacaatcgtg acatccattt
    17641 tgatgcgctt cattttcagc atgaaatctt gagaaatcaa agtattcgag ttactggcga
    17701 aaatcacgat tttttttgct tgaagcatta gttgtttggc tttttgcagt tggtcatgat
    17761 gaatgagaga aagtgtatca tgaatcgtca tctgttctaa agaagccatt ttatttgcga
    17821 tcgtcataat accgtctgtg gtggaaaaag gaagattcgc atctacgtct tgaaaattcc
    17881 cttcaagata ggctgtttca gctaaaaaag cattacgaaa actgacccag ccatcaaaac
    17941 ccattttttt tgctacacgg atgagtgtag aggggtggac ataacatcgc tcagcaatcg
    18001 cttggatggt catttcgcca agtgctggag aatcgcttaa gagaaactgg atcacttcat
    18061 tttctgaagg agaaaaggag atttctttga ttttttcacg taataacatg tagatacctt
    18121 cttcttattt ttttacgtaa attataactt ttttgaaaat gatttgtaaa cggatttata
    18181 ctatataact taaaatagac attttttgac aagatattta caaaaatcca ctagaaaaca
    18241 gtaaattttg taaatgttac tggaaagaaa cagatttcta ttgtttgttt tgtttacaaa
    18301 aaagataatg aaagtgtaaa aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc
    18361 gctacgattg gtggaggcag cagttacacc cccgagttga tggaaggatt tatcaaaaga
    18421 tatgaggaat taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag
    18481 cttgagatcg ttagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaag
    18541 atctatgcaa ctctggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag
    18601 tttcgtgtcg gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg
    18661 atgattgggc aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca
    18721 gtcgttttgg caatcgttga ggacatgaag cgtttatgtc caaatgcttg gctgatcaat
    18781 ttcgcgaacc caagtgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa
    18841 gtgattggat tatgcaatgt gccagtgatg gcgatgatga cagaaccagc aatgcttggc
    18901 aagaaaaaag aagagctgat ctacaagttt gccggtctaa atcatttcca ttggcacaaa
    18961 gtagcagata ataaaggaaa tgaccgcacg gcagaattga ttgatcgttt atacgatgaa
    19021 gagaatggat taccgaaaaa tatctttgat atcccgtttt tcaaagaaca attaaaacaa
    19081 atgaacatga tcccttgtgg ctaccaccgc tattactatc gtgaagaaga aatgctggaa
    19141 catgctttag aagaatatca aacgattggt acaagagcgc agcaagtgaa agaaacagaa
    19201 gcagagctat ttgaattata caaagatcca gaattagacc ataaacctga acaattgcaa
    19261 aaacgaggtg gcgcttatta ttcagacgct gcctgtgaaa cgattgcttc aatctatgga
    19321 aataaagaaa cacagatcgt ggtatctaca aagaatgatg gagctgttcc tgacctgccg
    19381 gcagattgtg tagtagaagt cactgcctat gttggcgcac aaggagcgag aaatgtagcg
    19441 tttggttctc ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg
    19501 ctgacgatcg aagcagctgt gactggtgat tatgggacag ctttacaggc gtttacgatc
    19561 aatccactta tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca
    19621 caccagcagt atttaccgca attcaaggag acaatcagcc gattaaaaga agaaggaatc
    19681 gaagttcaag atgaactggc aagagaatta tcaggtaata cacatataat ggaggaagta
    19741 taatgggatt cagaaaagat tttttatggg gcggtgctac agcagccaat caatgtgagg
    19801 gcggttatga tgaaggtgga cgcggactag caaacgtaga tgttgtgcca atcggaaaag
    19861 accgttttcc tgttatcacc ggaaagaaaa aaatgtttga atttgatgag gatcattttt
    19921 atccagcaaa aattgcaatc gatatgtatc atcgttataa agaagatatt gctttgtttg
    19981 cagaaatggg gttcaagact tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg
    20041 gggatgagaa agagccgaat gaagctggct tgaaatttta tgaggatgcc tttaaagaat
    20101 gccaaaagca cggaattgag ccacttgtga cgattacgca ttttgattgt ccaatgcatt
    20161 tgatcaaaga atatggtgcg tggcgttcca gagaactcgt tggtttttat gaaaatctgt
    20221 gtcgagtcat cttcaatcgc tataaaggac ttgtcaaata ttggctgaca ttcaatgaga
    20281 tcaatatgat ccttcatgca ccattcatgg gcgcagggct gtattttgaa aaaggagaaa
    20341 atgaagaaca aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa
    20401 cgaagattgc tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag
    20461 catattatcc atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag
    20521 aaaattattt cttcattgat gtccagtcac gaggagaata tcccgcttat gcattgaaag
    20581 aaatggagcg aaatggcatt cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag
    20641 aacatactgt tgattttatc tctttttctt attatgcttc ccgagtttcg actaccgaca
    20701 acgaactctt ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc
    20761 aagcaagtga atgggggtgg caaattgatc cattaggctt gagaataacg atgaatgata
    20821 tttatgatcg ttatcaaaaa ccattgttta ttgtagaaaa cggcttaggt gcagtcgata
    20881 cgccagatga aaatggttat gtggaagatg attaccgaat cgattacctg gcagcgcata
    20941 tccaggcgat gaaggatgca gtagaactag acggagtcga tctattaggc tatacgactt
    21001 ggggatgtat tgatttagta tcagctggta ccggtgagat gaagaaaagg tacggattca
    21061 tttatgtcga tcgtgataac caaggaaatg gcacattagc ccgttcaaag aaaaaatcat
    21121 tcaactggta taaaaaagta attgattcaa atggagaaaa tctttcgaac gattgatcaa
    21181 tgatttataa aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag
    21241 aaacgcaagc agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt
    21301 aagtaaagga gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga
    21361 tgactgctgc tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggtg
    21421 cccctggcgg ccaaatgaac aatacggcag aaatcgaaaa ttatcctgga ttcgattcga
    21481 tcatgggccc cgatctagct tataagatgt atgaaagtgt ctcaagattt gggacagaaa
    21541 atgcttatgg aatcgttcaa gatatcaagg atcacggcag ccataaagaa gtcatctgtg
    21601 aagataaaag ttacgaagga aaaacagtca ttatcgcaac aggatgtatc caccgtaaat
    21661 taggcgtacc tggcgaggaa gaatttgctg gacgaggcgt ttcttactgt gcagtctgtg
    21721 atggtgcgtt tttccgtaac aaacgtctag tagtcatcgg gggtggggac tcagcagtag
    21781 aagaagccat ctatttgaca caatttgctt ctgaagtagt gatcgtccac cgtagagatg
    21841 aattacgtgc acaaaagatc atccaagacc gtgcatttgc aaatgaaaaa atttccttta
    21901 tgtgggatac agtcgttgac gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag
    21961 cacgtaatgt caaaaccggc aatgaatttg atttagcagc agacggagta ttcatctatg
    22021 ttggactgga tccattaact gcacctttta gagaagcagg tttgacgaac gaagctggct
    22081 ggattccaac agacaacgaa atgaaaacga atatgccagg cgtttttgcc atcggagatg
    22141 ttcgtgaaaa agatttgcga caaatcacta cagctgttgg tgacggcggt attgctggac
    22201 aacaagtgta taaatacttg gagtctttag aagaaacaac agcataagtt taaaaagagt
    22261 agaagaacgg gttcaattgt tcttttactc ttttttttcg cgtgaaattt actgaatctg
    22321 atttatgcga acacgtccaa caccttttga taaaagaatc ctttcttgat ttaagcgttt
    22381 tttggtcctc atactttcat atctaataag tgaatgctat acttattaag aaccatattt
    22441 cagaaagagg tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag
    22501 aaaatttaga agaaaactta aaaaaacaat taaatgatct gggtgaagat cctgaaaaac
    22561 ttgaagatgc tttttatgca ccgttggaat tcggtactgc aggtatgcgg ggaatcttag
    22621 gacctgggat caatcgaatg aacatctata cggtaagaca agcaacagaa ggactagcac
    22681 gtttcatgga tacacaaggt gaagaaacaa aacgccgagg tgtcgctatc gcttatgact
    22741 cacgccatca gtcaccagaa tttgcaatgg aagcagccaa aacactagca catcacaata
    22801 tcccgtctta tgtattcgaa agtctgcgtc caacaccaga gctttctttt gcagttcgtc
    22861 atttgaagac gtttgcggga atcatggtaa ccgcatctca caacccagcg gcttacaatg
    22921 gatataaagt ctacggagaa gatggcggac aaatgcctcc tgcagacgca gatgcattga
    22981 cgaaatatgt ccgtgaagtc agcaatccgc taaaagtcga agtattatct gacgaagaag
    23041 ctaaacatag cgggttgatc acgatcatcg gagaagaagt ggacgctgct tatctagaag
    23101 aaatcaaagc agtgacgatt gatcgtgaat tggttgaaac aatggggaaa gatttgaaac
    23161 ttgtgtacac gccacttcat ggaacaggga aaatgttggg cgaacgtgcg ttgaaacaag
    23221 ctggattcga acaattcgta ctagttcccg aacaagctgt ggcagatcca gatttcacta
    23281 cggtcaaatc gccaaatcca gaagaacatt cagcttttga atatgctata cgacttggag
    23341 aaaaagaagg agctgacttg ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag
    23401 cggttcgttt gccagatggt tcttatgaag tattgactgg gaaccaaatc ggagcattgt
    23461 ttgctcaata tattttggaa gcacatcaat caagaggcac tttaccagaa aacgcagcgg
    23521 tattgaaatc aatcgtttcc agcgaattgc ctacagcaat tgcggaacac tacggtgcta
    23581 ccatgttcaa tgtgttgaca gggtttaaat tcattgctga aaaaatccaa caatttgaag
    23641 aagaccattc acatactttt atgtttggat tcgaagaaag ttatggttat ttagttaaac
    23701 cttttgttcg cgataaagac gcgatccaag cattgctttt attagcagaa gtagcagctt
    23761 actataaaaa acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacctatg
    23821 gctatttcgc agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcgaaaa
    23881 tctctgcttt gatggaaaaa ttccgtaaag aagcaccgac agaatttgcc ggagcaaaag
    23941 ttattcaaac agaagacttt aaagaattga cgagaacaga ccgtgaagga aacgtagaaa
    24001 aactaacaac accgccatcc gacgtattga agtatacatt ggaagaaaat ggctggattg
    24061 ctatccgccc ttcaggaact gagccgaaaa tcaagtttta tattggtgta aaaggaagtt
    24121 ctacagaaga cgcagccgaa aaagtagcct cttatgaagc gattatcaaa aagatgacag
    24181 aagaataaaa aacttgagac tgatggaaaa agtggtaaac tttagaagtc agtctttttt
    24241 caagttggag gtgaatggat tgccagacac gcaaaaatca aaagtagaga aagtcagtca
    24301 attattcaaa atgttgagtg atcccactcg tcttaaaatt ctgctatatc tgaaagacgg
    24361 ggaacaaaat gtgacagcaa tcacccaagc agtggaaatg gagcagtctg ctgtatcgca
    24421 tcaactacgt ttattaagag aaaatcatgt cgtcaagtct catcgcgagg gaaaagcgat
    24481 tttgtatagt cttgatgatt ctcatgtact ggatattttg aaccagacac tcaaacatgt
    24541 ggaacatcaa taacgatagg tgcaaataga taaacactgg agtgtgacaa cgaagaaatt
    24601 tcgttgtcac actccagtgt tttttaagaa aaagacaaaa atgatcaatc gcttttttct
    24661 gttgtttcag ttccataata actatgatct tctaggtcta aagcattcaa aataagcgaa
    24721 gccgtttctt caatagataa agatgctaca ttgatcacta cacatcctag tttttcataa
    24781 agatcattcg caaaatcgag ttcagctcta attttttcga tatcggaata agcagtatca
    24841 gggtttaatc cgtaagagcg catccgttct ttacggatat tatttaaaat atctgggtta
    24901 tttgttagtc ccacgatttt tttaggatcg atctcccaaa gctgtttggg aatatgtgct
    24961 tgtgggatca aaggtaaatt ggcaactttt aaatttttgt tagctaaaaa caagctcaaa
    25021 ggcgttttgg aagttcggga tacgcctagc aaaacgacat ctgcttctaa aaaaccacga
    25081 ggatctttcc catcatcgta ttttacagca aattccattg ctttgatccg tttgaaatag
    25141 ttctcattca ataaatggag ggcaccaggt tgccgaattg gcttgatacc agttaaacgt
    25201 tcaatttctg atatcggtgg cgtcatcaca tcaaaactaa ataattgatg ttcttcacaa
    25261 aaagaacgtg ttatctcgat cagttcttga ttgattaatg tatgcaggac aagtgctttt
    25321 tcttctaatg catcttgcaa tgcctgcctt aatgtttttt catctgtcac gaacgttctg
    25381 cggaataggt tgaactcaac ggtaggatac tgagccatag tagcttgcgc tagttttgtc
    25441 gcagtttctc cagcagagtc ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa
    25501 gtcatggata aaacctcact tgcatttttt ttatgtttat tataccatac tctagaatgc
    25561 gtacttcgta atcattttga tttggaggga taagtcatga aaaaacagac attgatcgaa
    25621 caatcattag aaacattgaa aaataacggt tttaaatata ctaaaaaaag agaagcattg
    25681 ttaacttatt tagtgaagcg taaccgctac gtatctgcta aagaagtttt tgactatatg
    25741 aatcaggaat tcaaaggggt aagttatgat acgatttacc ggaatctcca tgattttgca
    25801 gagatcgatt tactggaaga aaccgattta aatggagaaa tgaagtttcg ttttcattgc
    25861 tgtcagggga aaatcggcca tcaccatcat tttatctgta cagtgtgcgg aacaacgaaa
    25921 gaaatccata tgtgtccgat gaattatttc gaagaacagc taggtggatg cacgatcgaa
    25981 gggcatagat tcgagatttt cggaagatgt gaaaattgtc aataaggatt taaataaaat
    26041 aacatttgat aattttaaaa aaaagcaaga tttccttgac ggtcaaaaca gcattagata
    26101 taatatttac aggacagtct tgttttttga gatatatttc aataacaatt acggttactt
    26161 tttaagctcg gagggaggga attaacatgt caaaaacagt cgttcgcaaa aacgaatcac
    26221 ttgacgatgc tcttcgtcgc ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat
    26281 ctcgtaaacg tgaattctac gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag
    26341 ctagaaaacg caaaaaattc tagttctgtg atgaattgga gttggtagtg tgtcactact
    26401 aagtagatta aacgatgaca tgaaagcagc gatgaaagcg aaagataagg aatctttaca
    26461 agtcatccga atgatcaagt catctataca aaacgagcag attaaagaag gtcacgactt
    26521 gacagaagaa gaagaactaa cggtgttgtc tcgtgaaatg aaacagcgac gtgattcttt
    26581 gcatgagttt gaagaagcag gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt
    26641 aatagtcgaa aaatatatgc cggaacaact ttccgacgaa gaaattcgac aacttgttca
    26701 agaagcaatc actcaaactg gtgcttcatc aatgaaagaa tttggcaaag ttatgggtgc
    26761 aattatgcct aaagtcaaag gaaaagcaga tggaaaccaa gtgaatgcga ttgtgaaaga
    26821 actattacaa tcttaatttt atcatttaaa ctgcggacag tgattatccg cagttttttg
    26881 tgttcgtaaa aaaagaacta aaaaacagct agaagaaaga atcttattct t
//
LOCUS       DAZKSU010000037        26116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000037 DAZKSU010000000
VERSION     DAZKSU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.037"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACG8Z3_001569"
     CDS             complement(81..1532)
                     /gene="cls"
                     /locus_tag="ACG8Z3_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0823883.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1873..4539
                     /locus_tag="ACG8Z3_001570"
     CDS             1873..4539
                     /locus_tag="ACG8Z3_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0823884.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            4903..5655
                     /locus_tag="ACG8Z3_001571"
     CDS             4903..5655
                     /locus_tag="ACG8Z3_001571"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0823885.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5677..6831)
                     /locus_tag="ACG8Z3_001572"
     CDS             complement(5677..6831)
                     /locus_tag="ACG8Z3_001572"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0823886.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7016..9457
                     /locus_tag="ACG8Z3_001573"
     CDS             7016..9457
                     /locus_tag="ACG8Z3_001573"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0823887.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9787..10491)
                     /locus_tag="ACG8Z3_001574"
     CDS             complement(9787..10491)
                     /locus_tag="ACG8Z3_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823888.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10678..10923)
                     /locus_tag="ACG8Z3_001575"
     CDS             complement(10678..10923)
                     /locus_tag="ACG8Z3_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823889.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(10940..11791)
                     /locus_tag="ACG8Z3_001576"
     CDS             complement(10940..11791)
                     /locus_tag="ACG8Z3_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0823890.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            11957..12961
                     /locus_tag="ACG8Z3_001577"
     CDS             11957..12961
                     /locus_tag="ACG8Z3_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0823891.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13202..13558)
                     /locus_tag="ACG8Z3_001578"
     CDS             complement(13202..13558)
                     /locus_tag="ACG8Z3_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0823892.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            13926..15113
                     /gene="tuf"
                     /locus_tag="ACG8Z3_001579"
     CDS             13926..15113
                     /gene="tuf"
                     /locus_tag="ACG8Z3_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0823893.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15301..17892)
                     /gene="mprF"
                     /locus_tag="ACG8Z3_001580"
     CDS             complement(15301..17892)
                     /gene="mprF"
                     /locus_tag="ACG8Z3_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0823894.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(17918..18679)
                     /locus_tag="ACG8Z3_001581"
     CDS             complement(17918..18679)
                     /locus_tag="ACG8Z3_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0823895.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            18846..19478
                     /locus_tag="ACG8Z3_001582"
     CDS             18846..19478
                     /locus_tag="ACG8Z3_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFQ0823896.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKKHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            19537..20397
                     /locus_tag="ACG8Z3_001583"
     CDS             19537..20397
                     /locus_tag="ACG8Z3_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0823897.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20582..22897
                     /locus_tag="ACG8Z3_001584"
     CDS             20582..22897
                     /locus_tag="ACG8Z3_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0823898.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23079..24920
                     /gene="dnaG"
                     /locus_tag="ACG8Z3_001585"
     CDS             23079..24920
                     /gene="dnaG"
                     /locus_tag="ACG8Z3_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0823899.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            24944..26053
                     /gene="rpoD"
                     /locus_tag="ACG8Z3_001586"
     CDS             24944..26053
                     /gene="rpoD"
                     /locus_tag="ACG8Z3_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0823900.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAKQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8435 a         4643 c         5447 g         7591 t
ORIGIN      
        1 ttcggaagaa gggactgtga caagattttt gtcacagtcc ctttaatctt agtatatttt
       61 tctctacttg aataaaaaaa ttataggatt ggagaaaata atcgagaaat ttgttgcttg
      121 aataatagcc agttagacat ttccttgact gtttcagttg tcatggatga acaatgtgtc
      181 tcatcttctt tgaactgagt agcaagttct tctaaaaact caggatcgta aatgacagca
      241 cttgtttcaa aattcaatcg gtagctacga atatcttgat ttgctgaacc taccatgcag
      301 atttcatcat ccattatcaa tgtttttgca tggaggaagc cgttctcata gacaaggatt
      361 tgtacatttt ccttgatcag ctgccgagca taatattgtg ttgctcgata aataaaagga
      421 tgatccggtt tgttgggaat catgattttc acatctacac cagaggcagt tgcgattttt
      481 aaagcagcaa tgacactttc atcaggaaca aggtaaggtg tctgtatcca aacacgtttt
      541 ttagcagaag taatcaattt gataaatgaa agcttgattt gttccctttc gttattcggt
      601 ccgcttgaaa caagctggat ggatgtatca gcaagcttag attcatctcg atctgctttt
      661 ttaaagaaat aattcaaatg atagccgacc tttttttctt caggtaccga gacgttccag
      721 tctgttaaga agcgaagctg gagcaaagaa gaagcggcac cgaaaatccg tatatgcgta
      781 tcgcgccaat agccgaattt ttttgttgta tttacatatt gattggcaat attgaaacca
      841 ccggtataac taatctttcc atcgatcaca acgatttttc ggtgatcatg gtaattcaat
      901 cggaaacgaa gtaatgccct ttgtgaggta acaaatgtat ggacatgtcc accatttttg
      961 attagacgat tgaaatcttt tgcttttgtg ccatgagagc caaatgcatc atataatatt
     1021 cgaacttcca cgccttctgc ggctttttct tctaaaacat gtaagacttg ctggccgata
     1081 ttatctgtca caaatgcgta atattcgata tggatcgaat gtttggcttt ttttagatct
     1141 tgaagcagtg aatccaattt ctcttgtccg tctgtgagaa gagtgacaga attcattctt
     1201 gtcagcggca tacgatttaa tgaagagaag aagtcaacaa attgctgttt gtctttatcg
     1261 cccatgtcag gatcgtaatg ttcaatggta tctcctctaa aagactgaaa gttttctaat
     1321 tctttcaagt cactttgtcg gagatagaat tttttcttat ccgttaatcc acgtccgaaa
     1381 aataaatata atacaaagcc caccccagga agggcaaaca atactaagag ccatgcccaa
     1441 atggctgcta catctcgggg tttgatcaaa atagccacca aagcaataag tgcatttaat
     1501 agataaaggg cggttataat actagatacc acaaaaatgt tcctccttac atacaataac
     1561 attagtatag catgatagaa gaaagaagcc taccaaggtg aatatacttt tcactccttt
     1621 catgaaaatg taggtaaatc gggctaaagt agttgctttt tgtaggttaa taaggtagga
     1681 tattagtaca gttgaatatc tccttagggg agtaaccggc aacgtttgtt gcgataatat
     1741 caacagtaag ttaaccaaat ggttttctgg tattgtatga aatggtgaga cctaagacaa
     1801 atgcgtattt gtcttaggtc tctttttttt cagacaggac tagggccaag atcaaaaaat
     1861 gggggatgaa atttggaaaa caaggaacaa gcacaacaag cggtctataa agaaacgatc
     1921 gactcgttga tgcagcaaac gcagagctca accgatggat tgagcaccac gcaagcgaag
     1981 gaaaaattag aaagagatgg cttgaaccag ctaaaagaaa agccgaagaa gccaacttgg
     2041 aaattgttct tagaaacttt caaagatgca atggttatcg tattgctgat tgtcgcactg
     2101 attcagatgg tcatgggtga ctacatcgaa ttcttagtta tttttgcagt attgatgttg
     2161 aattctataa tcagtgttgt ccaaacaaaa aaagcagaag gttctttaga agcgttgaaa
     2221 aacctttcag cgcccgatgc tcgagtgatt cgtgatggta aggaaaaaac gattccggca
     2281 aacgaacttg tggtaggaga tatcgtgttg ttagacgcag gcgattatgt acctgctgat
     2341 ggacgtttac tagatgcggg atcattaaaa gtagatgaag gtatgctgac aggagagtcg
     2401 acttctgcag aaaaagaagt aacggatatc acaagtacgg taccaatcgg agatcgtaca
     2461 aatatggtat tcagtggaac gatcgtaact tacggacgcg gtaaattctt agtgacttca
     2521 actgggaata atacggagat cggacaagtt gctggccttt tggaatcaac aactgagcaa
     2581 gtcactccgt tacaacgagg tctggataaa ttcagtaaaa aactaagttt ggctattttg
     2641 gcattgtctt tattgatttt aggtatccaa ctgtttcgta tttatcttgg agaaggtaca
     2701 ggagatatga ctgcagatat cgtcagcgca gtgatgtttg ccgtagccgt agccgtagct
     2761 gctattccag aagcattaca gtctatcgta acaatcgtgt tatctttggg aacgaataaa
     2821 atggcaaaac gtcatgccat tattcggaag cttccagcag ttgaaacact tggttcgaca
     2881 agtatcatct gtacagataa gactggtaca ttgacacaaa acaaaatgac agttgttgac
     2941 tactttttag gtaatggcga tactggggat ttcacaaatg acccttcaaa atggacagct
     3001 gacgaacgtc gcttgatcga aatcagtgtt ttagcgaatg atgcatcgat cagtgaagac
     3061 ggtactaaat tgggcgaccc tacggaagta gcgtttatcg actttagtga aaaattaaac
     3121 cagccttatc aggaaatccg aaacaactac ccaagacaag cagaacttcc ttttgactca
     3181 gatcgaaaat taatgtctac tggacacaca attgataaca aaaattactt gtttgttaaa
     3241 ggcggaccag atatcgtctt ccaacgcagt aaaaaagtat tgctaaatgg tgaagtcgtt
     3301 ccgatgacgg atgaattgat gaaaaaattc caagagcaaa atgaagcttt ttcaacacgc
     3361 gcattacgtg tactagcttt tgcttaccgt ccacttgata attttgattt atcatttgaa
     3421 gatgaagacg atttgatcct tgttggtttg cttgctatga tcgatcctcc acgtgctgaa
     3481 gtgactcagg cggtaacaga agcaagaagt gcaggaatca aaacaatcat gattactggt
     3541 gaccacaaaa caacagctgt tgctatcgca aaacaaatcg ggatttttga agaaggaaac
     3601 ctggctttga ccgggacgga actagatgaa ttgacagaag atcaactaaa cgaagtttta
     3661 gaaaaagtca ctgtttatgc acgtgtttca cctgagaata aaattcgtat cgttcgtgct
     3721 tggcaaaata aaggtcatat ctcagctatg acaggtgatg gtgtaaatga cgcaccagca
     3781 ttgaaacaag cagacatcgg aattgctatg ggaacgggta cagatgtggc aaaagatgca
     3841 gcagcgatgg tattgacgga tgataacttt gcttctattg tcagcgcagt agaagtagga
     3901 cgtaatgtgt ttgataatat caagaaagct atttcttatc tatttgcagg aaatttaggt
     3961 gcgatcattg cgatcatctt tgccttagtt gctgactggt ccaatccatt tacagcatta
     4021 caactgttgt tcatcaattt agtcaatgat tcactgccag caattgcatt aggtacagaa
     4081 aaagctgaac ctgctacgat gaaccgcccg ccaagagatc caaattcagg aatctttacg
     4141 gacaaaacat tgatttctgt aacgtatcgt ggtgtgctga ttgggattgc tgttatcttt
     4201 gctcaatggc ttggcatgca gcattcacct gaattcggtg tagccatgtc attttcaaca
     4261 ttgatttggt gtcggacatt gcaaacattc ccagcacgtt caaacacaca aacagctatc
     4321 caagcagggt tcttctctaa cagaagtgtc ttactagctg tagttgtatg tagcgcgctt
     4381 tactgcttaa caatgatccc aggactgaaa gaagtcttct ctatcccatc agcatttgga
     4441 ttgacagaag tctttacatg tatgggaatc gcacttgtga caattgtttt gatggaaatc
     4501 actaaattga tcattgtacg agtacaagga aataaataaa acgaatcaac gaggttgtga
     4561 gcctgccgtt taattctgag tcacaaggtg caatttcaac aaacagcttc tcatgtttgt
     4621 tgaatattgt gacttgtgac cgaagagttg gcaggagaac accgtttaag gaagaagttg
     4681 taaaatcagc attttgagct aatactgagg gtcatctgat tggacactgt ttattcggaa
     4741 agagggactt tgacacgact ttggtcacag tccctttttt tatttatgat agacggaaag
     4801 ccaatcattt tattattcat caaaagagat agaatagata aaagaggaga atgatttatt
     4861 ccgaaaagtg aaaacatata acaaaaaatt agaggaaatc aaatgaatga aaaagaatgg
     4921 gatgaatttg ctgaagaata ttatgaaatc cagcaagaat cgcaagtaac tattgctgaa
     4981 gatgtaaaaa attatctgaa aacggagaaa gtcctgcctg ctgaaacagc agcagacgtt
     5041 gctggaggat ctggcagatt tctttcctta gcaaaagaaa ttaatcgcta tgatttgatc
     5101 gatttttctt caggaatgct agaaaatgca aaaaaagaag cagaaaaaaa tgagttgcca
     5161 aatgtttcct ttatcaaaca gtcgttagaa gaatttatcc aatcaaatga gacatatgaa
     5221 atggttttta cagcagcaaa tccagcattg gtacatctta ttcaattaga aaaattgatg
     5281 aaaaagtcaa gcaaatggtg cgtgataatc agagtagtgg aatcaatcga tgatgttttt
     5341 tatcctatcg aaaagcaact aggaatgata gaagaagatc ctaatacatc gccagttgta
     5401 atggatcaat ttgaaacata cttaattgat gaaggattca aagtgaagaa aaaagatttt
     5461 acttatttta cacaggaatc aattactgaa gaattgatga agctatacta tgacgcgtac
     5521 gcaaaagatc ctgttgtgac caggtacatt cgcaaactat tcagtgaaaa acgagaaatc
     5581 cttagtacga ctaagctgac ctatagactg ctcctttggt ccgcagataa aaaaagaacc
     5641 tttcacgact cgtgaagggt tctttttatg attttattat attttgtagt aacgaatatc
     5701 attttcgatc tttgtaatcg aaaaaggtac atctgaagta tattctgcac attctccgtg
     5761 ttcaggaagc gtctcactga aaatcgtttc atattcgtca acagtgatcc gtgttcgaga
     5821 atctagcatc tcttgatgag attgtgcgaa gagatattct tgatagtttt cttccagata
     5881 acctgtaaaa aattcactta ctgcaccaga accgtagctg aatagaccga tgcgatctcc
     5941 tggctgtagt gatttcgaat tttccagtag agaagttagc cccaggtata atgaaccagt
     6001 gtaaagatta ccgattcgtc ggctgtaacg gatgctttct tcatagcgag ccataagacg
     6061 ttcctgattt tcttcgtctg tttggtctaa tacgctttgc aatgcctttt ttcccatctt
     6121 agtatacgga atatggaaag caatcgcctg ataatcttcg agtcctcgac ccgacaattc
     6181 tttatgtcga ttccaaactt tttggaatga ttcgatatac gtagaattag ataaaggacc
     6241 atcaacaaca ggaaattcgc tataatctgg acgccagaaa tcatagatat cttctgtcag
     6301 aaatacgctg tcgtcttcaa tcgataaaat acgcgggttt tgagtgatca tcatcgcaac
     6361 agcaccgaca ccttgcgtca cttcaccacc gcttgccaag ccgtaacgag caatatcact
     6421 tgctatgact agtacttttc gttctggatg atttttgaca tattctttcg ccatgtgcaa
     6481 agctgcggtg ccaccgtagc aagcctcttt tacttcaaaa gaacgagcaa aagattggat
     6541 tttcagtaaa tggtggataa ttaccgcact cgcttttgaa tgatcaatcc cggattctgt
     6601 tccgacgatg accatgtcga ttagctcacg gtctttttct gttacgattt tgctggcagc
     6661 atttgctcct agtgtaatga tgtcttcatt tgcacgattg actgccattt ggtcttgacc
     6721 aatccctata tggtattttg caggatcatc cccacggctt tctgccaact ctgtcatatc
     6781 taaatataaa ttaggaataa aaaaggaaag acgatcaatc cctattttca tttcaacacc
     6841 tctttactta tgataaaaaa cttactttac taaggtatca taaaaagatt aaaagaaata
     6901 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
     6961 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
     7021 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
     7081 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
     7141 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
     7201 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
     7261 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
     7321 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
     7381 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
     7441 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
     7501 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
     7561 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
     7621 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
     7681 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
     7741 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
     7801 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
     7861 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     7921 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     7981 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     8041 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     8101 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     8161 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     8221 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     8281 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     8341 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     8401 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     8461 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     8521 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     8581 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     8641 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     8701 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     8761 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     8821 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     8881 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     8941 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     9001 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     9061 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     9121 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     9181 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     9241 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     9301 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     9361 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     9421 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     9481 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     9541 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     9601 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     9661 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     9721 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     9781 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     9841 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     9901 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     9961 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
    10021 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
    10081 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
    10141 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
    10201 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
    10261 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
    10321 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
    10381 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
    10441 caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca tatgttctct
    10501 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
    10561 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
    10621 aagaaaaata aaaaatggaa cggattatgt atatatacat aatccgttcc attctttcta
    10681 ttttttcggt atcaaggcaa aaacaggtag ttcaggtgaa taaatgactt catgttcgtg
    10741 ccagggtttg accattagcg aatgatcgaa ccgttttttc agttcttcga tgatttgatc
    10801 atgtgtccag tttcttgcag gaaaatgttg gattttctct gcatcgatca aaaagacaaa
    10861 atcggaatga aaagtctggg taacttcttc cgagagagaa ttcaagcgtt cgattggatc
    10921 cataaaatca cctttcgact taaaattggg tagtatctgg gtcttttgct ttgccgacta
    10981 caccatctaa ctgatcgatt tgtttcattt cttcttctgt taattcaaaa tcaaataatt
    11041 cagtattttc tttgattctg cttggtgtca ctgacttagg aagaggaagg aattcgtgtt
    11101 gcagcgacca acgcaaagca acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt
    11161 tcatttcagg cacatcgaag attttaccag tgcctaatgg actatatgct tcgaggatca
    11221 tatcatgttt ttttgcatac tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa
    11281 cttgattgac catcggcata atttttgctg tttttgctag tgtatctaga tggtggggta
    11341 agaaattgct cacaccgatt gctttgattt tacctgcttc atacagttct tcaaatgctc
    11401 gccatgtatc tgcatttgct tgttcccaat tatcacgaaa agcaacaggg tttggccagt
    11461 ggatcaaaaa cagatctaaa taatctgttt ggagcttctt caatgattct tcaaaagaag
    11521 acatcacaag ttcatagctg tggtttgcat tccataattt tgttgtcaag aatatctctt
    11581 ctctaggaat tccactttcc ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt
    11641 gggcagtatc gatatggcga taacctgcag ccagcgcttc tttaacagag gaaacagcga
    11701 catccccatc aggtgtttgc catgtgccga atcctactac cgggatttcg taaccatttg
    11761 atagacgata agtgcttgtt aatgaattca tactttcagc tcctttttta ttcgttcaac
    11821 tataagtgta gcgcattttt cgaaaaaaag aatccatttt gtttctactc ttgaaatttt
    11881 tgtttaaaag aaaatgattt tgattctggt atttttgatt ttttttccgt aaactaagac
    11941 agaagggaat gagaaaatgg atatctattt aaaaaaagca attctgcata ttattgacag
    12001 agaaactggt tctccagtct tttcacaaaa agagctggat ctgacaaaag aatatattcg
    12061 tgattttctt caaaagaaga tccaaaaaat ctcttctgct caaacaaaaa ctggacaact
    12121 agcagaagaa agcatgatcg ttgaatcact taaaatcgct caagaaaatt ttgtggaagc
    12181 aagtgaagca atcgttcagc ggtggttcga tatctaccaa gaaagtgaag aagcacctag
    12241 tgcagatgta tttgttgtct tgtatgaatt agatacagtg atgtacttat ctttattgaa
    12301 agtcaattat cgtgaggctt acactcactt tgttgaagca gacgaagcag ggatcgacaa
    12361 ccgcttgatt ttgaatcgag caattctagg ggcgaaaaca caaaaagcag atgaagcatt
    12421 cgctgtgaat ttgactgatt ggacctacga attaattgaa aaaaaatatg agttttctgg
    12481 caaaaaagag ctttattttt ctggaagagt gatcgaaagt gttcctgcac cttctttaga
    12541 agaaaatgta aaagtgatcc aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga
    12601 agaatttgat atcatggctg atttgaaaga agctgtctat gatacgatag aagaaaaagg
    12661 tcggttggat catgagatga ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat
    12721 ggcttttaaa gaagaagtac aagagcaagg atttgtccca gaagcacctc ctgtaaaaga
    12781 agtacaagaa atttctgaga aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat
    12841 cgaactgatc gtaccagtag atgtatatcg caacccagat ttgattgaat ttgtgaataa
    12901 tccggatggt acgatatccg tgatgatcaa aaatgtggat gaagtattga atcgtttata
    12961 aaaatgacta gaatattagg tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag
    13021 agaaggatcg gaaaagacga atataacaaa aaaagagcgt gagacaaaag taaagaacac
    13081 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcttattt
    13141 cttggagtta aacgcttctg tcccagcctc tttttatcta ataaaatttt aagaccattt
    13201 actaattatc gattttttct gccgcatttt taggcacttc agaagccttt atttcttccc
    13261 agcttaatac acctttacga ggattgactt tgagtttaag atacgcattc atctttaatg
    13321 gatgttctct ttcttcgcga agcgtcacct gttttttttc accatcttta ttataagccg
    13381 cttgcttgta ggtataagtc gtgaatttct ctccgttatc tgcttgggta gctgacattt
    13441 cgccattcgt cgtgattttt gtataataag cttcgccgcc gtaaaaataa tgataagctg
    13501 caaaacttcc gccgagaaat atgcctaaaa cgacaagtac gccaattaat tttttcatgt
    13561 acttcacctc tttccttatt attaacgaaa tagaagattt cgtctattga aagttgttgg
    13621 caacagctga attttaaact tttcctcatt ttgttcttat tgcttcaaaa gaaagttaag
    13681 gactatcttg aagtttttct caaaaaacac atttttttat ttataaaagg tacttttttc
    13741 gcgtataaga cagacgagtg ctcttagttg actttgacca atattgacgt ataataagct
    13801 tagttcgaat cttacaaaaa gtaagtcgga cgtattcttt ttgatttagt caaaaaaaga
    13861 ctataataaa aaggtgagag ctgaaatttt tcagtgatct atattattta ggaggtttgt
    13921 aaattatggc aaaagaacat tatgacagaa gtaaaccaca cgttaacatt ggtacgattg
    13981 gacacgttga ccatgggaaa actactttaa ctgctgcgat cactacagta ttaggcaaaa
    14041 aaggcctagc aaatcctcaa gactatgcta gtattgatgc agctccagaa gaacgtgaac
    14101 gtgggattac gatcaatacc gcacacgtag aatatgaaac agaaaaacgc cactacgcgc
    14161 atattgatgc accaggacac gcggactatg tgaaaaacat gattacaggg gcagcacaaa
    14221 tggatggtgc aatcttagtt gtttctgcaa ctgacggtcc gatgcctcaa acacgtgaac
    14281 acattttatt gtcacgccaa gttggtgtaa aatacctgat tgttttcttg aacaaagttg
    14341 atttagtcga tgatgaagaa ttgatcgatt tggtagaaat ggaagttcgc gagttattga
    14401 gcgaatatgg tttcccaggc gatgacactc ctgtgatcaa aggttccgca ttaaaagcat
    14461 tgcaaggcga tccagatgct gaagctgcta ttatggaatt gatggataca gtagatgaat
    14521 atatcccaac accagaacgt gatacagata aaccattact attgccagtg gaagacgtct
    14581 tctcaattac aggtcgagga actgttgcct caggtcgtat tgatcgtggt gctgttcgtg
    14641 tcggtgatga ggtagagatc gtagggatca aacctgaaac acaaaaagca gttgtaacag
    14701 gtgtagaaat gttccgtaaa acgttagatt acggggaagc tggggataac gtaggcgtgt
    14761 tgttacgggg gatccaacgt gacgatatcg aacgtggaca agtacttgct aaaccaggtt
    14821 ccattactcc acatacaaaa ttcaaagcag aagtgtacgt gttgacaaaa gaagaaggtg
    14881 gacgtcatac tccattcttc aacaactatc gtccacagtt ctacttccgc acaactgatg
    14941 ttacaggaac aatcacattg ccagaagata cagaaatggt catgcctggt gacaacgtga
    15001 cgattgatgt tgacttgatc catccaatcg ctgttgaaaa cggaacgaca ttctcaattc
    15061 gtgaaggcgg acggacagta ggttcaggta tcgttacaga gatcgaagca taagaaacgc
    15121 gactcgaata ttttggtaat gaaaaatgtt taaaataaaa gagcggtcca aacaggattt
    15181 ctgtttggac cgctctttgt tcttcaattg tataagaaga gattgtagaa gcatgaactt
    15241 tcgttttact tcagtctctt ttattcatca tgagagtgtt catctatcgg tatgcagttt
    15301 ttaatacttt cttcgtatcc attttttcaa accacttatt ctaccttgtg tctgctcaac
    15361 tggcgcatta tcaataatca gcaaagcaat cataacatat gcaatccaac tatctctaga
    15421 atatagagta taacgtgaga cccagtttgt agcgtacttg cttttatatt ctcttaatcc
    15481 atggaaagaa taaaaatgag aaccaaactc gtaaaccaag tatgcaatcc tttcctgtaa
    15541 aaaactcttt ctggaagttc cgacatttga aagaggggcc atccctaaat tgaaccattg
    15601 gatatgttct tctttcatat aggcaaacaa atgcaagaac aagaaatcca tactgccaga
    15661 aggtgctttt ttagaatcat atcgcatcag atcaatcgtt cctactttat tgttcgtgta
    15721 agtaggcatg atcgttgcaa aagcgacaat ttcttcttgg gtatttttga caactgcaat
    15781 aggcgctctt tgtagataat cttcagagaa aaagcctaaa gaaaaccctt tttcctttcg
    15841 tgaacccaac caattatcag agatattttt gaaagtactc atttgctcag tagaaaatgg
    15901 tggttgtaag acatcaaatg taaatccttc tcgttcgatc cgattcaaga cagcacgagt
    15961 gcccttcatt tttttccctg aagttgtaaa actattcagg tctacataag cttcttcccc
    16021 catcttaatg aaatcgtagc cgaattcatg caaaatcata acaatctctt cgcttgtttc
    16081 gtagaaaact ggtaaatagc atagtcgatc tgtttcttcg ataaaagctt cgattgcttc
    16141 tggaaaatct tctttcttac cagaaggatc acccattaca atacatttat tattgaagca
    16201 agcgaattgg agaagaacag ttggttcacc gtctttttca taggcaaaca tccgcttatc
    16261 ttttaagaag atcaattgac tatcggagtt tcctccataa gttgtaagga tcttcagggc
    16321 cttttcttca ttaaaggctt cgccaatctg tttcttttct ccttgaagga aatgaacaaa
    16381 tagaacgatc atgaaactaa cagcgatgat ggccaacaat cccgaaaacc aaatcttttc
    16441 agaaggaaat aagaaaaagg aaataaaatg gtgacgccga tgagggaaat ctggcagatt
    16501 gtatacccca ataacgatgt aaagcaagga cagtgcacca ataatgattc catcgatcgt
    16561 acgccactcc catgaataga tcaattgttc ccgaaataat tcgtttttag aagcaagaat
    16621 gatcagaagc agaatagaca agaaaaccac cggtgtgaaa ctaaaatcac ttaataaaac
    16681 ataaagtaaa gctagaacaa tcaagaagat tgtaggcaaa tatgctcgtt tcacacgtgc
    16741 tgcgatccct ctgcccataa tgattaaaag aaatcccaat agtatactag gaaattggat
    16801 aataaaatga aatttcaatg ggttcaaact atgaagccag cgaaactctg taaaagcttg
    16861 cgggatagtg gcagaaagta ctagcatgat tccagaaaag tataataaga caaccacgat
    16921 tttatgcgca atttctgttg ctaattgttt agggatacca gaataacgct gatcaaaagt
    16981 ggaaccgata tttttgaaga aaaagacgat tccaatcaaa aacggaatga tgtagtagaa
    17041 caggcgatag agcaagatcc acacaacgac tatttctctt ggcacaccaa tagcggacaa
    17101 cccgataatc atcattacat cgaaactccc aatttctcct ggaatcatag acacgattcc
    17161 tatcactgaa gcagcaatga ataatggaat tgtttgccac agatcaatcc ggatttccat
    17221 taaataacca acagaaagga aactaaacag gacaccggtc cattccaaca atgaaactag
    17281 caatagctgt ccttgagttt taagtgaggt accaccaagg aatcctttct ttttgaaagt
    17341 agtaaataaa aagacgacag gaaaatatag accgccgccg atcaaccata tccaatattg
    17401 cttcaaaaaa ggagcaactg gcgtgaagac tacaagtaaa aaagaaagca gactgtaaat
    17461 agaaagtcca gccatcaaaa acaagaaaac tttcgataat gcttggatca cgtttctgct
    17521 ctctttcttt tgtccatata gttcagagcg aagccccaca ctaacaaacc cgccgaaacc
    17581 ggcaatattg ttgatcgtat tgatcagcca gcttgtctca aacaggtaac gaggattctg
    17641 cttttgttca agcatccgat tcaaaataat atcataacca accataggta acacagagat
    17701 aagaccaatc aatagcatta gtccgctttt ccatacaggt atagtggcaa acgtttctcc
    17761 taattgctgg atagatagag ttttaccgat ggacatcaat tcaccgacaa taatgacaat
    17821 gacagaaatc aaaaagattg ttttgaacag tcctaggtgg tttttcagcc attgtatggt
    17881 attttttaac aaataaatca ccttctattc ctaatagtta tgtttcagaa agaaattgaa
    17941 gaacttctcg attgaagcgt tcaggatctt ttctagctaa ttcatgtcct tgttctttca
    18001 caagcacaaa ggaagctttt ggtatagttt tagctatata caaagaatgt tttagcttga
    18061 ttacatcttt ttttcccacg atgatcaaag tgggggagtt gatttttttt aaatcatttt
    18121 ctgtaagccc gatgtcatgc agcaaaagtt tgataactaa aagatttttg cgaagacttg
    18181 ggcgaaataa agagagtaac catacccatg cataatgaat attactgatg actctggcag
    18241 aaaaacgaac gcctttaaca agcgtgtttc cagaatttaa aatcaatcga tgaacttttt
    18301 tagggaagct gctggcaaat accagtgcta gatttgctcc atcactaaat ccaagaaaat
    18361 ctgcctgatc gattttttcc agcaacatga tcgtattcag atcctcggcc attaactgaa
    18421 aattcaacat agatgcttcg tttgtcgaac gaccatgtcc tcggctatct actagataca
    18481 cggtatagta gcgttcgagt acagggacct gttctgaaaa aaagcggccg ctgccgtcat
    18541 tgccatgcag taaaaagaga gggaagcctt gaccgctctt ttcatagtaa atttcgctcc
    18601 catctggcat ttttgcatat ttcttttctt ttttcacata atcacctact gtgtaaagta
    18661 taacgattat atcagtcatt gggcaattgt ttttactgaa aaacaacgat cattttcgta
    18721 agatgtcaaa aaatacagga aaattcattt tttcttaagg cgtacacaga aatcaaggaa
    18781 gaaatagcca agaagataaa gaaatagtaa gataaaaaaa gatgtcaaaa taactaggtg
    18841 atgaaatgaa gcgaaaacga ttatctatta tccgtattct tttttacatg atcattctga
    18901 cggttattgg tgcggtgttg tttttaggag gaagaactta tcgttcggta aaacgagtgg
    18961 aaagctatca aatagaaata acaacagctg caaaaaatta tcatatggaa aactacgaga
    19021 atttgatcaa ggcgataata ttgaccgaat caaaaggaaa agggacagat ctcatgcaga
    19081 gctcggaaag tctgcatgga gaagctgaga tcattgataa tccagctgac agtattgatc
    19141 aaggtgtcaa atatttggcg gaaatgatag tagaagcaaa gaaacaagga tgcgatttat
    19201 ggacagctgt ccaagcatat aattttggga aagattatat tgcctatgta aaaaaacatg
    19261 gcggagaaaa cacagtagaa ttagcagaag cgtattctaa agatgtgctg agcccacttt
    19321 taggaaatga ggaagggaaa caataccgat attggcggat ccagtccttg ctttacaatg
    19381 gcggctatct ctatcataat ggaggtaaca tgttttatcc tgatattgtc aaaatgaatg
    19441 aaaaaataat agagacctat gaaaaagtct ttaagtaaaa tagcaggaaa tgttcaatca
    19501 tgaataaaat taaatgaaag aaggagtgcg caaatagtgg aaaaaaggca aaaaatggaa
    19561 aatcaagaag tggggtacac aaccaaagac tggatgttac ggtttgtgaa gggaatgttt
    19621 atcggttcag gatttatctt gccaggagtc agcggtggtg cattagcagc tgtctttggc
    19681 atgtatgagc ggctgatttc ttttttagcc caccttacaa aaaatttcaa agaaaatgtt
    19741 ttgttttttc tgcctgttgg tctaggcgga gtgacaggca tctttctact ttctttttta
    19801 gtcagttttt tattaggtgc gtatgaaaca acgatattat ggttttttgt cggatgtatc
    19861 gctggaactg ttcctgcgtt atggaaagaa gcagggaaaa aaggaagaga tacttctgat
    19921 gtgatcatca tggttgttac ttttgttctg gcttatttgt ttttattata cggcgctcaa
    19981 ttatttgacg ggcaagtcga tcagaatttt ggaacatggc tgattgccgg cggattgatt
    20041 ggtttgggta tgatcgtccc aggattgagc ccttctaatt ttttggtgta tatgggattg
    20101 tataaagcga tggctgacgg atttaaagaa gtacgacttg aagtgattgt gccgatcgga
    20161 attggtggga ttctttgtgt tcttggattg tcaaaaatca tggattacat tttctcaaag
    20221 gcatatagca agctattcca tttcatttta ggtgtagttt ttgcttcaac gattatgatc
    20281 attcctaccg attacgcggg attgggagtt gctggggtac tagcttgtcc tgttcttttt
    20341 atagccggta cagcactagg ttggtggatg agccgtttgg aggaacaata taagtaaaga
    20401 tagattgata ataaaaacag cacacgggtg attcaaacaa aaaggttggc atcttttttg
    20461 taaaatcatt cgtgtgtttt tctataaatt ttataaaaac aagttgtttt taatcttgga
    20521 acacgctaga cttaaacacg tgattatttt tgaaagttgg cggaaaatgg agaaaaagaa
    20581 aatgaatgga cttacacagc aagaggtagt tcagaaaatg gcttctggcc agcaaaatga
    20641 ctaccaggaa gatgcatcaa aaagtacaaa acaaatattc agcgataata tattgacgtt
    20701 attcaatttt ctgaactttg gtattgggat ttgtctgtta tttgtaggtg cgtattccaa
    20761 catggcgtat ttagcaatta ttgtcgtcaa tatcgttatt ggaatttatc aagaaataca
    20821 tgcaagaaat cttgtacgga aattatcgat tgtttcccaa tcagaagtac tagtgatccg
    20881 ggacggtaaa gaacaatcga tttctactaa agagctggta ttaggagatt tggtaaaaat
    20941 cggagcagga gaacagatac cagccgattt acaggttata tctggacggg cagaagcaaa
    21001 tgaagctctt ttgacaggcg aatctgattt gattgtcaaa gaaactggag ctgaattgct
    21061 ttctggaagc tttttgacaa gcggttctgt catggctgaa gttatccgag taggtgcaga
    21121 taattatgct gtccgcttga ctagcgaggc aaagactcac aaagctattc gttctgaact
    21181 tgtagattcg attcgcaaag tatccaaatt cactagtttc gtgattattc ctttggggat
    21241 catactattt ttggaagcac tgttcatccg cgatgcttct atcaaaattt ctgttattgc
    21301 atctgcagca gctttgttag gcatgcttcc aaagggtttg gtattgctaa taagtattgc
    21361 attaacgaca ggggtaacca aactggcaaa aaaacgaatc cttgttcaag acatgtattc
    21421 aattgaaacg ctggcccatg tcgatacatt atgcttggat aaaacaggga ccattactga
    21481 agggaaaatg agtatccagc gggtagaagc actacatccg ctgtacgaga aagccatccc
    21541 agaaattatg gggacgtatc tggcagaaag cagtgataac aacatcacta tgcgggctat
    21601 gagagattat tttgagcttt ctaatcggta tgatgtgaca aacaaagtcg cattttcttc
    21661 tgatcgcaaa tggggcgcga tggaatttcc agaactggga gtcgtgtacg taggagcgcc
    21721 tgaacgtttg gtggaagaag atcggcttcc ggacagtcta aaaacagccc aagcgaatgg
    21781 ctatcgcgta cttatgttgg ggatcgctcc aaatcagcaa ttgaacgaag aaaaaccagc
    21841 atttgttgag ccggttgctc tgtttgaaat cgatgatcca atccgaaaaa acgctcaaga
    21901 aacactagct tatcttcatg aggaaggtgt ggatctgaaa gtgatttctg gagacaatcc
    21961 ttttactgtc tccaatatcg ctagacgtgc aggattaccg aattatgatg cgtatgtcga
    22021 tctttctcaa attactgaag aaatggaagt gcgagaagct gctcatcgct atacggtttt
    22081 tggacgagtg agtccgcagc aaaagaaatt gctggtaaat gaattgaaag aaagcggcag
    22141 aacggttgca atgacaggcg acggtgtgaa tgatgttttg gcgttaagag aagcagattg
    22201 cagcattgct atggcagaag gtgatggagc aacacgtcag attgcgaatc tggtattgtt
    22261 ggactctgat tttactacat tgccagaagt cctgtttgaa ggacgccgag tggtcaacaa
    22321 tgtgacgaaa gtctcaggca tcttttttat caaaacgatt tattcattta ttttatcgat
    22381 tatatgtgcc gttacagcaa ttggatttcc gtttatccct atccaaatca cgctgattga
    22441 tttagcgatt gaagggtatc cttccttctt tttatctttt gaacaagata aaagaaaaat
    22501 cacttaccgt tatttgccta cagcacttat taatgcttta ccaaatgcct tgcttgtagt
    22561 agtgaatatc attgcggtgt atctacttgg aaaaacattc gcatggggaa atgctgaaac
    22621 tactactttg atgtattact tgctgattgg gatcagttgc atggcagttg taaaagcttg
    22681 ttatcccttc aacccattgc gcatcttcct agcagtaacg acgatcgtag ggatatatgt
    22741 agctgcaatg ctgttccatg gaattttgga agtaaactta gggttgggag agagcgtagg
    22801 atacttcttt gtattcatgc tcgtaaatat tgttcttcga gtattgtact ggaaggctga
    22861 tattcaaaat cagttactga aaagagtagc aaattgatgc ttaatgagat agaaatgtgt
    22921 tatacttgaa aaagcaatga aatatgatac aatcaaacat gtataattgt gtcctttaaa
    22981 atcgcactga tttcagttag tgcgattttt taccggttat tctcctgaaa tatcttctgt
    23041 taaacccaaa agatatcaat agagcagggg gtgaaaaaat ggctcaacgg attcctcaag
    23101 aggttatcga aacagtacgc agtcaaacga atatcgttga ggtgattgga caatatgtcc
    23161 agttgaaaaa gtctggtaaa aattacttgg ggctgtgtcc ttttcatgaa gaacgtacac
    23221 cgtctttttc tgttgcagaa gacaagcaga tttttcattg ctttggttgt ggtaaaggcg
    23281 gaaacgtgtt tacttttctt caagaactag aaggtctaag ttttccagaa gcagtggtaa
    23341 aagtggcaga cttcgaacag ataccgttag aagatcaatg gcgtcagcgt actatagctg
    23401 tccaaaatcc tgaatctgct acggggaaac tgattgctgc tcatgaaaaa gcagcggaaa
    23461 tctatcatca catgctattg catacaaaag ctggacagcc agctttagaa tatttgcaaa
    23521 gtcgtgggtt gtctttagaa ttaattgaag aattcaacat aggttttgca cctaatgagc
    23581 gttcttttct tcagcaagta ttcaagaatg aatcaatttc aaatgaattg ttagaacgct
    23641 ctggattgtt tgtcacgcat gatgatggcc gtatgtctga tcgtttttat caaagagtga
    23701 tgtttccact tcgtaattct caaggccaga cgatcggttt ttcaggacgc tggcttgcac
    23761 ctgaaaaagg gcaggaaaaa gatcagccca aatacttgaa tagtccagaa acagaactgt
    23821 tcaataaaag acaagtttta tttaatcttg ataaagcaag aagcgatatc cggaaaaacg
    23881 gagaaatttt gttgttcgaa gggtttatgg atgtgattgc tgcatggcag gctggtatca
    23941 aaactggatt agcttctatg gggacaagtc taacagctca acagatccaa caaatggaaa
    24001 agctgacgaa agaattggtc ttttgctatg acggagacaa cgcaggtttt gaagcaacca
    24061 atcgagggat tgaattgctt cggcaaaaca gtcgattagc actttctgtc gtagtcgttc
    24121 cagaaaagct tgatcctgat gaatatctcc gtaagtatgg agaagcatct ttccaagaat
    24181 tagtggctca tgggcgcgaa acagtcttta cttttaaatc acgttattta aaacaaggga
    24241 aaaatttaga taatgaaaaa gacaagatcg aatacctcga agaattacta gttgaactta
    24301 gccgagttgt ttcaccaatc gaacaagata tgtatctatc tcaactgtca acagagtttc
    24361 agatcggcag agagacgata caaaaacaac tgcgcgatct tcgaaggaca aatcagcaaa
    24421 atcgtcaaga acctcaggaa agtcaacaca ctcaggttac gagacaagaa actgcacgga
    24481 aaaagagacc tttgacgcaa gtagaaaagg cagagcagat tctattatat cgtgcattta
    24541 aagaagtttc tgtccgtaat attttaaaag aacgagaatt tcagtttatt catgatgcat
    24601 atgcagaagt ttatttttta ttcgatgcat atgtctcaca acattatgaa ttcaaccttg
    24661 cagagttttt agactttttg caagatgaaa atttaaggcg gttggtggtg gaaatcgaat
    24721 atttaccggt ggcagaagaa agttcgccac gagaaatcca agacttgcta gatgtgatca
    24781 gcaaatctgg cttagctgat gaaataacat taaaaaaaca aatgcagcaa gaagccagac
    24841 gaactggaaa caaacagctt gagcttgaat tgacgattga aatcatcaat ttaacgaaac
    24901 agttaaaaca agccaaatag ctactcaagg ggggccttct tttatggcaa atggaacaga
    24961 taaaaaaacg tatgaagcag cagtagcagc tttcatcaaa gaaaacaaac ccaaacgaca
    25021 agttgtatat gatgatttat ccaatcagct tgctacacca ttcactttga atgcagatga
    25081 aatggacaaa ttgatccaaa agattgaaga cgcaggtatc agtgtggtgg atgaaaatgg
    25141 tgatccatca attcacagct tgaagagtgc tgagaaaaaa gctaaacaag caaaaacaga
    25201 agatctttct gcgccaaccg gtgtaaaaat caatgacccg gttcgtatgt atctgaaaga
    25261 aattggccgt gtctcccttt taactgcgga agaagaagta gcgttagcat tgaaaatcga
    25321 agaaggcgac caagaagcga aacagcgtct ggcagaagca aaccttcgtt tagtagtaag
    25381 tatcgctaaa cgttatgtag gacgaggcat gcagttcctt gacttgatcc aagaaggaaa
    25441 catgggactg atgaaggcag ttgaaaaatt tgactaccgc aaaggattta agttttcaac
    25501 ctatgctaca tggtggattc gtcaagccat cactcgagca atcgctgacc aagcacgtac
    25561 gatccgtatt ccagttcata tggttgaaac aattaataaa ttgatacgta tccaacgtca
    25621 attgctgcaa gaccttggaa gagaacctac gccagaagaa atcggtgcgg aaatggatct
    25681 tccaacagaa aaagtccgag aaatcttgaa aatcgcgcaa gaacctgttt cccttgaaac
    25741 gccaatcggt gaagaagatg actctcactt aggtgatttc atagaagacc aggaagcaac
    25801 aagtccagca gaacacgcag cttatgagtt actaaaagaa caattggaag atgtactaga
    25861 tacattgact gatcgggaag agaatgtcct tcgcttacgt tttggtttag atgatggccg
    25921 gacgagaaca ttggaagaag tagggaaagt atttggtgtt actcgggaac gtattcgtca
    25981 gatcgaggct aaagcattaa gaaaattgcg ccatccttct cgttcaaaac aattaaaaga
    26041 tttcttagaa taagcgtgga ctattgacca gaagagaaat cagtaaaact gtttggttta
    26101 ttttaaaaga aaaaaa
//
LOCUS       DAZKSU010000038        25281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000038 DAZKSU010000000
VERSION     DAZKSU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.038"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..432)
                     /locus_tag="ACG8Z3_001587"
     CDS             complement(55..432)
                     /locus_tag="ACG8Z3_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823901.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            688..1416
                     /gene="purC"
                     /locus_tag="ACG8Z3_001588"
     CDS             688..1416
                     /gene="purC"
                     /locus_tag="ACG8Z3_001588"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0823902.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1416..1670
                     /gene="purS"
                     /locus_tag="ACG8Z3_001589"
     CDS             1416..1670
                     /gene="purS"
                     /locus_tag="ACG8Z3_001589"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0823903.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1672..2343
                     /gene="purQ"
                     /locus_tag="ACG8Z3_001590"
     CDS             1672..2343
                     /gene="purQ"
                     /locus_tag="ACG8Z3_001590"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0823904.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2344..4566
                     /gene="purL"
                     /locus_tag="ACG8Z3_001591"
     CDS             2344..4566
                     /gene="purL"
                     /locus_tag="ACG8Z3_001591"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0823905.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4551..5990
                     /gene="purF"
                     /locus_tag="ACG8Z3_001592"
     CDS             4551..5990
                     /gene="purF"
                     /locus_tag="ACG8Z3_001592"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0823906.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6013..7065
                     /gene="purM"
                     /locus_tag="ACG8Z3_001593"
     CDS             6013..7065
                     /gene="purM"
                     /locus_tag="ACG8Z3_001593"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0823907.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7062..7640
                     /gene="purN"
                     /locus_tag="ACG8Z3_001594"
     CDS             7062..7640
                     /gene="purN"
                     /locus_tag="ACG8Z3_001594"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0823908.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7637..9172
                     /gene="purH"
                     /locus_tag="ACG8Z3_001595"
     CDS             7637..9172
                     /gene="purH"
                     /locus_tag="ACG8Z3_001595"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0823909.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9188..10462
                     /gene="purD"
                     /locus_tag="ACG8Z3_001596"
     CDS             9188..10462
                     /gene="purD"
                     /locus_tag="ACG8Z3_001596"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0823910.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10584..11498
                     /locus_tag="ACG8Z3_001597"
     CDS             10584..11498
                     /locus_tag="ACG8Z3_001597"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0823911.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11631..13196)
                     /locus_tag="ACG8Z3_001598"
     CDS             complement(11631..13196)
                     /locus_tag="ACG8Z3_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823912.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13189..14640)
                     /locus_tag="ACG8Z3_001599"
     CDS             complement(13189..14640)
                     /locus_tag="ACG8Z3_001599"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0823913.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14654..15670)
                     /locus_tag="ACG8Z3_001600"
     CDS             complement(14654..15670)
                     /locus_tag="ACG8Z3_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0823914.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15890..16978
                     /gene="chvE"
                     /locus_tag="ACG8Z3_001601"
     CDS             15890..16978
                     /gene="chvE"
                     /locus_tag="ACG8Z3_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0823915.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17032..18558
                     /gene="mmsA"
                     /locus_tag="ACG8Z3_001602"
     CDS             17032..18558
                     /gene="mmsA"
                     /locus_tag="ACG8Z3_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0823916.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18560..19765
                     /gene="mmsB"
                     /locus_tag="ACG8Z3_001603"
     CDS             18560..19765
                     /gene="mmsB"
                     /locus_tag="ACG8Z3_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0823917.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20022..20999
                     /gene="guaC"
                     /locus_tag="ACG8Z3_001604"
     CDS             20022..20999
                     /gene="guaC"
                     /locus_tag="ACG8Z3_001604"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0823918.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21263..22687
                     /locus_tag="ACG8Z3_001605"
     CDS             21263..22687
                     /locus_tag="ACG8Z3_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0823919.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22729..22986)
                     /locus_tag="ACG8Z3_001606"
     CDS             complement(22729..22986)
                     /locus_tag="ACG8Z3_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823920.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23148..24257
                     /gene="pdhA"
                     /locus_tag="ACG8Z3_001607"
     CDS             23148..24257
                     /gene="pdhA"
                     /locus_tag="ACG8Z3_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0823921.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24260..25237
                     /locus_tag="ACG8Z3_001608"
     CDS             24260..25237
                     /locus_tag="ACG8Z3_001608"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0823922.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8178 a         4304 c         5684 g         7115 t
ORIGIN      
        1 tttaaaagcg gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc
       61 gtaaatggct tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc
      121 gagaaaagcg tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt
      181 gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata
      241 aagaaaagtc cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa
      301 tgtataaaaa ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt
      361 atatagacag gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat
      421 tgcatcttcc atcggcaagt gcctcctcga acccattaac caaattttta gttaattcta
      481 atataccatc attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg
      541 ataaaacacg aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat
      601 aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt
      661 taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa
      721 gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa
      781 gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat
      841 caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt
      901 gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg
      961 gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa
     1021 gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt
     1081 atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat cagtgagctg
     1141 aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg
     1201 gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat
     1261 gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa
     1321 gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt
     1381 ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa
     1441 cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta
     1501 tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg
     1561 atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg
     1621 taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt
     1681 gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag
     1741 atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat
     1801 ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct
     1861 cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc
     1921 ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga
     1981 cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg
     2041 gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa
     2101 ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc
     2161 tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa
     2221 gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga
     2281 tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta
     2341 tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc
     2401 gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg
     2461 cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa
     2521 aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct
     2581 ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa
     2641 agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga
     2701 atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg
     2761 ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc
     2821 ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct
     2881 tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat
     2941 atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca
     3001 ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa
     3061 cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg
     3121 tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca
     3181 gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg
     3241 acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg
     3301 gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa
     3361 ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa
     3421 tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa
     3481 gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag
     3541 ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag
     3601 caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc
     3661 aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa
     3721 gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga
     3781 ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg
     3841 gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta
     3901 tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca
     3961 ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt
     4021 ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca
     4081 ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa
     4141 aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag
     4201 agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac
     4261 tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg
     4321 atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt
     4381 gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct
     4441 gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag
     4501 atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg
     4561 aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg
     4621 ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta
     4681 tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg
     4741 tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac
     4801 gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc
     4861 atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa
     4921 aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc
     4981 atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg
     5041 aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg
     5101 acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg
     5161 cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg
     5221 gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc
     5281 agctttctat ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag
     5341 ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag
     5401 taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg
     5461 cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa
     5521 catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg
     5581 tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta
     5641 cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga
     5701 ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta
     5761 aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt
     5821 ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc
     5881 cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact
     5941 atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca
     6001 agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa
     6061 gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt
     6121 atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa
     6181 gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa
     6241 gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta
     6301 gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg
     6361 ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg
     6421 ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta
     6481 gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac
     6541 ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt
     6601 gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg
     6661 aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt
     6721 ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta
     6781 gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg
     6841 ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg
     6901 tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa
     6961 gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc
     7021 gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg
     7081 cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg
     7141 aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa
     7201 ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt
     7261 atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg
     7321 gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata
     7381 tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag
     7441 ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac
     7501 caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag
     7561 aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa
     7621 atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt
     7681 agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa
     7741 gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc
     7801 tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg
     7861 aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga
     7921 ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat
     7981 agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa
     8041 aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga
     8101 attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt
     8161 tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga
     8221 aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga
     8281 aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc
     8341 agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc
     8401 tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa
     8461 tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc
     8521 agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac
     8581 agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga
     8641 agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca
     8701 ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga
     8761 tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac
     8821 tccagcagaa gcaaaagcat tagtgtttgc ttggagagct gtcaaacatg tgaaatctaa
     8881 tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg
     8941 agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt
     9001 agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat
     9061 aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa
     9121 taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt
     9181 cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa
     9241 acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac
     9301 aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt
     9361 tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg
     9421 gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc
     9481 tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac
     9541 agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg
     9601 tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac
     9661 cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga
     9721 tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc
     9781 gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt
     9841 tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat
     9901 ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat
     9961 gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga
    10021 aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct
    10081 ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt
    10141 agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta
    10201 ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt
    10261 ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta
    10321 tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt
    10381 aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa
    10441 tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata
    10501 atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg
    10561 tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag
    10621 cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga
    10681 ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg
    10741 gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc
    10801 tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac
    10861 atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt
    10921 tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg
    10981 aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa
    11041 tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct
    11101 tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta
    11161 tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat
    11221 ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg
    11281 aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg
    11341 cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg
    11401 tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt
    11461 acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa
    11521 agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct
    11581 gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt
    11641 ttgtttttaa ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta
    11701 aataaataac tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata
    11761 cgatagtctg attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa
    11821 tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat
    11881 gcaacttgtt gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata
    11941 aaatccaatg catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga
    12001 gcagttaaga aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg
    12061 attgctaagc gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct
    12121 ttcaggcaga catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga
    12181 gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat
    12241 aatgaagaca aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct
    12301 tcttgaatca aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt
    12361 atcatctgcg tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca
    12421 atggcgacag aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct
    12481 acctgataac tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg
    12541 aatcgagagg aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa
    12601 taatcactga gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat
    12661 acggtcattt ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca
    12721 ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa
    12781 acaagatcag aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt
    12841 tctattgctt gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt
    12901 acctggattg ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga
    12961 gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt
    13021 tggatatcag tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca
    13081 ccatctccag cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt
    13141 tcccgcaagt tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt
    13201 gtttcctcca attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg
    13261 gtatttgctg tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt
    13321 gtttacgttg taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc
    13381 tttacgtatt tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa
    13441 ttggatcatc tcatcaattt tgcatgtggt aatacgaata aatcctttgt gacggacgta
    13501 cttcgttcca tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga
    13561 aacaggaaaa agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat
    13621 ttttaagatg actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc
    13681 ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc
    13741 ttcttcaaag cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa
    13801 atggggagta atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt
    13861 caaagcgttc tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt
    13921 aagtgtttgt aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga
    13981 aagttgttct gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa
    14041 gcgacgattc tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt
    14101 taatagaatg agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac
    14161 acgaacaaaa tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat
    14221 gatctcttca ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg
    14281 ataatcttcc agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag
    14341 tgtatttgta ttatttttgc tctctcgaat atcattccta agttcttcta ggatattttc
    14401 ctcattgaac tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac
    14461 tttttgatca accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg
    14521 ataaatcaaa agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa
    14581 tagaagcatg gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat
    14641 gatacttctc cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga
    14701 cagtctcgga aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat
    14761 aacgagtaac ggtattggct acataccagc cgacatcagg tttacactcg actacacaat
    14821 tgacgattcc tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga
    14881 tcaaatcttc tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca
    14941 tttcatcact atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc
    15001 gtttcatctg gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag
    15061 tcatcggagg tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg
    15121 attccaaaaa tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt
    15181 ctaggattct gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg
    15241 cccgatttcc ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat
    15301 tcgtttgaat atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta
    15361 atacagagtc ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg
    15421 caatgaacgt ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga
    15481 gaacttggta atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc
    15541 agctactctc cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg
    15601 tattaggtgt acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa
    15661 cacgtttcac tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg
    15721 gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag
    15781 acaaaaaaag tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc
    15841 tttctttatt agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa
    15901 atttagaaag attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg
    15961 tggcaattca ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa
    16021 atcagcagaa agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg
    16081 ttataaaaca gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga
    16141 aaatatgatt acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt
    16201 aactgatgtg ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt
    16261 actgatgaat tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt
    16321 atcgcaagca gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac
    16381 gattgaatta tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt
    16441 gatgtctgtt ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac
    16501 gagtttcagt caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga
    16561 taatctgtta agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga
    16621 tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa
    16681 acctttacct gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc
    16741 cggggaacaa acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga
    16801 aatgatcaaa gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga
    16861 taatggggtg aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa
    16921 ttatcaagca gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga
    16981 ggaaccaaac aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat
    17041 tgtattttaa aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc
    17101 gtgaatctga tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga
    17161 aaatcaacat tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa
    17221 atcttttatg atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc
    17281 atcgtcatca ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt
    17341 tttctcggaa atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa
    17401 acatatgaac tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag
    17461 attggcgtag ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt
    17521 ctgttgattt tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg
    17581 agattgatcc gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg
    17641 aatgaaatcg tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag
    17701 acactagaaa atgaaatgat cagcgaagag catattattc gcggaatggt gggcagagat
    17761 ttagttgatc gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa
    17821 gactggacgg tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg
    17881 catttgaaaa aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa
    17941 ttcgctatga gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag
    18001 gaaggggcag agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat
    18061 gtctcggagg accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg
    18121 acgctgagca gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta
    18181 actgaggcag aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt
    18241 gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca
    18301 caacccgata ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat
    18361 gagatctaca cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct
    18421 tctgaacttc ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt
    18481 attacaggag aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg
    18541 aaagaagagg tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa
    18601 actaaatttg aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt
    18661 agcgggacta cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa
    18721 tataactaac cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt
    18781 agttgtgctc ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc
    18841 aattgcgggc atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg
    18901 tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat
    18961 accggcgttt atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt
    19021 tctaggaggc caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta
    19081 tttaccagac ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat
    19141 catcacgatc gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt
    19201 atttgaagtg gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt
    19261 actaggaata acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct
    19321 tggtttgatc gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat
    19381 ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat
    19441 cacgttctgg gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc
    19501 tgctcgattg aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc
    19561 ggcagtatat tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat
    19621 tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt
    19681 agactggcag caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta
    19741 taataagaaa aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca
    19801 agacatttgt cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag
    19861 actaaaaata atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt
    19921 tttgcttaat tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat
    19981 gttcgtgttt tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg
    20041 aagatattca attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa
    20101 cggtcacatt aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga
    20161 ttattgatga aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc
    20221 ggtttgatga agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta
    20281 cctcgatcag tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag
    20341 aatcgctaaa tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta
    20401 ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg
    20461 ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg
    20521 ggattggtcc aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct
    20581 ggcaactggc agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg
    20641 gtgggatcag aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca
    20701 tgattggttc attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg
    20761 gaatcgtgta taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa
    20821 atgttgaagg aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg
    20881 aaatgcaaca agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca
    20941 ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa
    21001 aaataaataa aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag
    21061 ccaaacaaaa atagttcttt atatgttttg tttggctgga attattatcg cagatacaag
    21121 cttttgaaca tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta
    21181 tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat
    21241 agacaataga aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta
    21301 tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag
    21361 tcaacttgaa tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt
    21421 ccatgcattg gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg
    21481 gtttgttaat cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat
    21541 gatctctcct cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca
    21601 gatccaaaaa acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc
    21661 gaattttgag tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga
    21721 tcttttaggc tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga
    21781 attggcattt tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt
    21841 gattttacaa acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt
    21901 ttaccgatta cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga
    21961 agaagggatc gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt
    22021 gcttatcaac ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga
    22081 aaagctagta gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac
    22141 ggatctttca tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa
    22201 tgtgttatac gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt
    22261 gataggacct gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc
    22321 gatgaatatc cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa
    22381 aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga
    22441 ttcacaatcg tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg
    22501 ggatgggaaa aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat
    22561 tgcaccaagt ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct
    22621 ggatgaaaac caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac
    22681 agaataattt cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag
    22741 ctgtttcttt tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt
    22801 tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc
    22861 aacgttttta aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga
    22921 tgaaatataa aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat
    22981 tggcataatt tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc
    23041 atgataattt attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc
    23101 gcagaatgaa gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa
    23161 aagcaccgat cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc
    23221 aagcacttga tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg
    23281 atgaactcgt tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa
    23341 cagcgctgaa ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa
    23401 gtcagctggc tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg
    23461 atgtacctca actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag
    23521 gacatgctgc aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca
    23581 tcggtgcaca atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga
    23641 aaaatgtcgt cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag
    23701 caatcaattt cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt
    23761 atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg
    23821 tcgcagctgg gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg
    23881 tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac
    23941 tgacttatcg ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa
    24001 aagacacaga tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga
    24061 cggaaaaagg actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag
    24121 aaatcaaagc agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct
    24181 tgaaaaacat gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag
    24241 cgaaggagtc gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt
    24301 agcacttgaa ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa
    24361 cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt
    24421 cgatactcct ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg
    24481 atttcgtcca gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt
    24541 agtagctcaa atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac
    24601 aattcgttcg ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga
    24661 aggtttgatc gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc
    24721 aaaaggacta ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat
    24781 gaaactatat cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga
    24841 taaagcagca gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt
    24901 gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat
    24961 tgatcttcgt acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac
    25021 tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt
    25081 ttctgagatc tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc
    25141 acctgataca gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga
    25201 tatcgaagac aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt
    25261 catggcttat caatttaaat t
//
LOCUS       DAZKSU010000039        24359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000039 DAZKSU010000000
VERSION     DAZKSU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.039"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..1426)
                     /gene="purB"
                     /locus_tag="ACG8Z3_001609"
     CDS             complement(131..1426)
                     /gene="purB"
                     /locus_tag="ACG8Z3_001609"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0823923.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1439..2563)
                     /gene="purK"
                     /locus_tag="ACG8Z3_001610"
     CDS             complement(1439..2563)
                     /gene="purK"
                     /locus_tag="ACG8Z3_001610"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0823924.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2556..3044)
                     /gene="purE"
                     /locus_tag="ACG8Z3_001611"
     CDS             complement(2556..3044)
                     /gene="purE"
                     /locus_tag="ACG8Z3_001611"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0823925.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LLFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3460..4767)
                     /locus_tag="ACG8Z3_001612"
     CDS             complement(3460..4767)
                     /locus_tag="ACG8Z3_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0823926.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4770..5351)
                     /locus_tag="ACG8Z3_001613"
     CDS             complement(4770..5351)
                     /locus_tag="ACG8Z3_001613"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0823927.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5467..5564)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5923..7215)
                     /locus_tag="ACG8Z3_001614"
     CDS             complement(5923..7215)
                     /locus_tag="ACG8Z3_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0823928.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7212..7835)
                     /locus_tag="ACG8Z3_001615"
     CDS             complement(7212..7835)
                     /locus_tag="ACG8Z3_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823929.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7838..8674)
                     /locus_tag="ACG8Z3_001616"
     CDS             complement(7838..8674)
                     /locus_tag="ACG8Z3_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0823930.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8691..9578)
                     /locus_tag="ACG8Z3_001617"
     CDS             complement(8691..9578)
                     /locus_tag="ACG8Z3_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0823931.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9594..10877)
                     /locus_tag="ACG8Z3_001618"
     CDS             complement(9594..10877)
                     /locus_tag="ACG8Z3_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0823932.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11560..12243)
                     /locus_tag="ACG8Z3_001619"
     CDS             complement(11560..12243)
                     /locus_tag="ACG8Z3_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0823933.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12261..12878)
                     /locus_tag="ACG8Z3_001620"
     CDS             complement(12261..12878)
                     /locus_tag="ACG8Z3_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0823934.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12888..13712)
                     /locus_tag="ACG8Z3_001621"
     CDS             complement(12888..13712)
                     /locus_tag="ACG8Z3_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0823935.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13705..14265)
                     /locus_tag="ACG8Z3_001622"
     CDS             complement(13705..14265)
                     /locus_tag="ACG8Z3_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823936.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14439..15395
                     /locus_tag="ACG8Z3_001623"
     CDS             14439..15395
                     /locus_tag="ACG8Z3_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0823937.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15700..16656)
                     /locus_tag="ACG8Z3_001624"
     CDS             complement(15700..16656)
                     /locus_tag="ACG8Z3_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0823938.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16760..17362
                     /locus_tag="ACG8Z3_001625"
     CDS             16760..17362
                     /locus_tag="ACG8Z3_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0823939.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17450..18949)
                     /locus_tag="ACG8Z3_001626"
     CDS             complement(17450..18949)
                     /locus_tag="ACG8Z3_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0823940.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18965..19489)
                     /locus_tag="ACG8Z3_001627"
     CDS             complement(18965..19489)
                     /locus_tag="ACG8Z3_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0823941.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19787..20536
                     /locus_tag="ACG8Z3_001628"
     CDS             19787..20536
                     /locus_tag="ACG8Z3_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0823942.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20527..21312
                     /locus_tag="ACG8Z3_001629"
     CDS             20527..21312
                     /locus_tag="ACG8Z3_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0823943.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21281..21997
                     /locus_tag="ACG8Z3_001630"
     CDS             21281..21997
                     /locus_tag="ACG8Z3_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0823944.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22099..23118)
                     /locus_tag="ACG8Z3_001631"
     CDS             complement(22099..23118)
                     /locus_tag="ACG8Z3_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0823945.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23137..24138)
                     /locus_tag="ACG8Z3_001632"
     CDS             complement(23137..24138)
                     /locus_tag="ACG8Z3_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0823946.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7231 a         5237 c         4237 g         7654 t
ORIGIN      
        1 ttaaaatcag gttcctatta ttgataagaa aatataataa actaaaagaa gcagaaccgc
       61 taacggatct gcttcttttt tggttatttt tgctattcta ttttaattga attttgcaac
      121 tcactcttct ttattccaaa cctactcgtt tgaatatcac gtcaacgttt ttcaaatgat
      181 gatgataatc aaaggcatcg tctatttctt ctttagataa taccgatgtg atggttggat
      241 cttcttctaa cagcgggcgg aactgcactt ggttgtccca agcgtacgca gtttttggct
      301 gaacaagatc ataagcttct tctcttgtca ttcccttatc gatcaatttc agcataacgc
      361 gctggctata aatcaaacca aatgttgcag acatattccg cttcatgttt tcaggaaaga
      421 ccgttagatt tttaacgata ttgccaaaac gatttaacat gtaattcaat aaaatcgtgg
      481 tatcaggcaa gatgattcgt tctgctgaag aatgagaaat atctctttca tgccataatg
      541 tgacattttc ataggcagtg accatgtgtc cgcgcaccac tctggctaga ccagccatat
      601 tttctgaacc gatcggattc cttttgtgcg gcatagcaga agaacctttt tgacctttcg
      661 caaaaaattc ttctacttca cgtgtttcgg atttttgcaa tccgcgtatc tctgttgcaa
      721 aacgttcaat acttgtagca ataagcgcca tcgctgagaa atattcagca tgtagatccc
      781 gagggagaac ttgagtagaa atttcttgtg gacggatccc caattgttta catacatact
      841 cttcaataaa tggcgggata tttgcgaacg ttcccactgc tcctgagatt tttcctgctt
      901 ctactccttt tgccgcatgt tcaaatcgtt cgatgttccg tttcatttct gaataccatg
      961 tggcaagttt caatccaaaa gtagttggtt cagcatggac accgtgtgtc cgacccatca
     1021 tcactgtata tttgtgttct tttgcttttt cgccaatgat ttccgtaaaa cgtttcaagt
     1081 cttcccgtaa aatatcattt gcttgcttga tgagatagcc ataagcagta tctacgacat
     1141 ccgtacttgt tagaccataa tggacccatt ttctttcttc tcccaatgtt tcagaaaccg
     1201 ctcgtgtaaa cgcaacgaca tcatggcgtg ttaatttttc gatttccaag atccgatcga
     1261 tatcaaatga tgcatgctca cgaatttttt tcacatcttc ttgcgggatt tcaccaagtt
     1321 ctgcccaagc ttcatctgct aaaatctcga cttcgagcca cgcacggtat ttgttctctt
     1381 ccgtccagat attccccatt tcagggcgtg tataacgttc taacatgttt tttctcctct
     1441 aatcccaaat attcgtttgt ttgatttctt ccagcgtttg ctggatgtct tctgtcaaga
     1501 tagtgatatg tcccatcttg cgaccttttt ttgcttgttt ttttccatag aaatggaatt
     1561 gccagtctgg cttattctca atcaagcgat agctttccag cagttgttct cccaaaacat
     1621 tgaccattac tgcattgctt atcaaacgta ctttttctcc taatggccaa ccacaaatac
     1681 ctcgaatatg ggcatcgaac tgactcatag aacaagcttc aatagaataa tgtccagaat
     1741 tgtgaggacg aggagctaat tcattcacat atagcccgcc tgtttttgtt aaaaacattt
     1801 ctacccctaa aacacctgaa aggctcactg cttctgcaat cacacgggca atacgctcag
     1861 cctcctcgat cacatcctga tcaatcgcgg ctggagcaat cgtttcatga agaatattgt
     1921 tgtggtggat gttttccacg accggaaaag tcgtgaaatc tccttgacca tttcctgcca
     1981 ccatgatcga aatctctttt tcaaacggga tccatgcttc taaaacacag gttccttctc
     2041 ttaaaaggtc catggcagga gctagatccg cccgactttt caaaacatac tgccccttgc
     2101 catcatagcc gcctcgagtg gttttcaaaa cacaaggata gccgatcccg tcaatcgcat
     2161 cttggatatc cgtcggactg acgatcgttg cataaggagc aatcacgatg ttattcgttt
     2221 ccaaaaaaga tttttctaac aggcggtctt gcgtaatcgc aagcaagtct gtcccttgag
     2281 gaataaatga catgggtaaa atcgcattta acgcctctac actgacgttt tcgaattcat
     2341 aggtaatgac atctgttctt ctggcaagct ctttcagcgc aaaggtgtca tcatagacac
     2401 tttcaatatg ccaatctgca acttgtgcgg ctggacaatc gctcacagga tccagcacac
     2461 ctactttaaa gcccatttct tttgcactaa tcgccatcat acgacctaat tgaccgccac
     2521 cgacgatccc gatcgttgaa ccaggcaaca aaggtttaac caagttgatc actactttct
     2581 ataacagttt tagccaacat tgaacgtctt tcagccaatt tagcctccaa ttctaaatca
     2641 tacatggaaa gcatctgtat cgcaagtagt ccagcattaa ttgctcccgc ttttccgatt
     2701 gctgtagtag ctacaggtac tcctccaggc atttggacaa tcgaaagaag cgaatccaaa
     2761 ccatttaatg ttcgtgattg gaccggtaca ccgataaccg gcaaagttgt ttttgatgcg
     2821 accatacccg gcagatgcgc ggctccccct gctcctgcaa taatgatttt gattcctttt
     2881 tttcgagcgt tttgtgcata ttcaaacagc aaatctgggg ttcgatgagc agaaacgacc
     2941 tttttttcat aagacacacc gaattcatcc aatatttgac atgcttcttt cattgtttcc
     3001 caatctgaag tactccccat aatcacagag atttttgctg tcattgtttt tcctcccagt
     3061 ttttctacat tcatatttta acaatcactt tttttgatgt caaagaaatt tcgaatgtta
     3121 acaaagaaaa acattttatt gttcgtgttt attaaagata aaaaaaagga atgtgacaaa
     3181 agttaggtca cattcctttt tatggataaa cggtgttctc ctgccaactc ctcggtcaca
     3241 aggaacaata ttcaacaaac atgagaagct tttttttgaa attgttcctt gtgactcgga
     3301 gatgaacggc aggctcacga cctcttcctt caacggtgtt caatcagctg accctcggta
     3361 ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc tctaacaatc
     3421 aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga aggcttcttc
     3481 caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga tagccgtaat
     3541 gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca tttgactgaa
     3601 taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa caatcaatag
     3661 atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga ctgccaccat
     3721 cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt gtgctaacgc
     3781 accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat agatgggttt
     3841 tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc cagtataagg
     3901 gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat atcccttttt
     3961 caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat atacacctgt
     4021 tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc atacgtcgaa
     4081 agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat ctccaatcgc
     4141 gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta aaccaatcaa
     4201 taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa ttaggagaat
     4261 cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg ttgcattacc
     4321 tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg tgataaccgt
     4381 tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag aaactaacac
     4441 aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt cattggttcc
     4501 gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag gcaagccaat
     4561 tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca taaagatatc
     4621 aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac cgatcaaaag
     4681 aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta aaaccgcagc
     4741 ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa ctcaacttgt
     4801 ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat ttcttcaagt
     4861 aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc tacttgcgct
     4921 ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc caagaaatcg
     4981 tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga aatcgtactt
     5041 gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc catcgtcaaa
     5101 ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc aaataaagct
     5161 ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc tttaaatact
     5221 tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg agtgacgaag
     5281 ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat acgttcaact
     5341 aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac cgcttttgtg
     5401 caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat aagaaaagaa
     5461 gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc tagaaactgt
     5521 catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg taaattcatt
     5581 ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa aatgttgttt
     5641 caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt tacttatttt
     5701 tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt aaatagttat
     5761 ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga caaaaagcat
     5821 gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg caataaggca
     5881 agattttata aactacggga agtaatttcc gtaaatctat tcttattctt ttaagctgac
     5941 aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg cgattcctgt
     6001 caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat cttcttcttt
     6061 ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc cgcccgcttc
     6121 ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga tggactgaag
     6181 aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact tttcgatcac
     6241 tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt cccacggcat
     6301 atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg ttacgcctag
     6361 tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat atccactgtc
     6421 tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt ctatagcaaa
     6481 tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt ctactccgcg
     6541 tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata ccttttgggg
     6601 acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat gaacaagaag
     6661 cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc cgtgttgcgt
     6721 atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac tttctatcag
     6781 ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc gcaggcttct
     6841 tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga tagtcagagg
     6901 tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat catgatgcgt
     6961 tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta gttgctcaaa
     7021 ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa gtatccgatt
     7081 tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac aagtttgatg
     7141 atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc cgacttgtac
     7201 atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac tgtgacaaat
     7261 ggcagccctg gatacagtaa tgcagtgagt aaaaaatcat gctgtataca ccagaacagt
     7321 ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa cagccagata
     7381 ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat atcaattagc
     7441 gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg aatcagatac
     7501 caagctccaa tctttcccac taataaatac caatcaactg caaaaataat gacaaacagc
     7561 tgaaacaacg taatgatgaa acacatccgt ttgatttttc ctttcctcca acataccttt
     7621 ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa aaaataaatc
     7681 agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc tagaactaaa
     7741 gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata gtagttgagc
     7801 ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga ccgcttgtac
     7861 tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc ggtagcagcg
     7921 acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg tctgcaaata
     7981 atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc aatggtccca
     8041 tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc gaaggtttca
     8101 gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca ataatcgctg
     8161 attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa atgaaaaatg
     8221 cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa ttcagccgat
     8281 tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc gttactaaca
     8341 tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg taagcaatac
     8401 aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac gaattcctaa
     8461 agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa ttttcccatt
     8521 gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc gttttaaaag
     8581 aacctgcgat catccataag accggataaa tcatgactag acctaatagc aaaataaaac
     8641 cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat tcacacaacg
     8701 acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat caacagcatg
     8761 atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg gttatacaca
     8821 taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat aaatgcctgc
     8881 gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat gatcggcgaa
     8941 agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc gatctctgcc
     9001 gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat cattgccgaa
     9061 ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg atcgctaaac
     9121 caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc gtttttagaa
     9181 aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact aggcacataa
     9241 tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata agcaatcaat
     9301 agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt gactcggatg
     9361 gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc gatccatgag
     9421 actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat catcggtatc
     9481 actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc gaccacattg
     9541 tcttgatcca agaaccgttt tagtttttct tttttcatgc cttcctcctg ctgctatcct
     9601 agaatgtttg acgcaaactc ataaagactc tctgctgctt cttctgctgt tacttcttca
     9661 taaatgactt tttcaactaa aagattgtat tggtcttgaa tttccggatt caaagcacta
     9721 tcaaagacat tcacttcgcc gttgtccata tcggaaatca tttctaaata atcaaatgtc
     9781 gcttgcaaag ttgcatcatc tgcacgagcg agctgttctc gaatattcga catgattggt
     9841 acaccacgtt ctccttttaa aatttcattg gcttcttcac tgttttgaaa gaaatcaata
     9901 aacttagcag cttcttcttt atgggctgag ctttttgcca tactcagcat ttgcgaagaa
     9961 cgtaaaggga taccagattc tccatctgct tttctgcgag gtggattaat caactgaatc
    10021 tccatatctt caggagcccc tgacaatatt tctgtcaact ggttacttga tagaaacacc
    10081 attgccgctt ctctatccct aaccgcctga aaagacagat ccgtcgtact tgcagctcca
    10141 ccacctggtt ctggatgggc ccctttgtct accagatttt tacgcagctg gaaaaaatct
    10201 gttagatagt ctggattttc aaatcccaag ctgttagata gatcttcccg attataaaaa
    10261 ttcaatcctt tctcaacttg tggtatttga ataatacagc cagctataaa atcttcgaat
    10321 cttgaaatag catagattcc taattcttca gtgatttgat agctgatttg ttcaaattca
    10381 tcccatgtcc aatttgaagc tggttcaggg atatttaatt gttcaaatat atttttattg
    10441 taagcaatcg caaacgaatt caccccgtta gacagtccaa ctaattgccc gtcgtaagtc
    10501 gtcgttgcta aataggattc atctgcatta gaaatatcaa ttatcccttg ctccacatat
    10561 tcatctaatg gttcaatctg attgatgtat tgaggaaatt gattgttcag ttggaaaatg
    10621 tcccagatat catctgatgc tgctagtgta cctaattttg tgtaatatcc ttcataatca
    10681 taaaattcat aatcgatcgt cacatgggga tttttttctt cataaagctc gattactttt
    10741 actgttgcat cgtgtcggac ttgcgaaccc caccaagcca tgttcagcgt gatttttttg
    10801 tcatcttctt gcccacaagc cgtcaagaag aataacataa cacttaaagc taagatgatt
    10861 ctcattcttt tcttcattct ctgcacctct acttccattg ttatttttca atatactgta
    10921 tttttataca gaacatattt attaaacgat ctttttcaag gggaataaaa accaagactt
    10981 gccttatcgt tctgcctagg atgaacaact caatactttc aagaaaaatt agatagtgct
    11041 gattcttctt tcatcgctaa aaacacttct tgcttcaatg atgggaggat aattacgcaa
    11101 agaaaaaagg atatcagaca actgcccaat atccttgaca tagctaaata agcatgcttc
    11161 ttcgtgtgcg ctcttcccta aaaaacagaa aaataactag taattctgta tgaaattagg
    11221 tacaatatac acgtcgatgc acaagcattg actatggcaa cttgattggt cagttcccgc
    11281 tgacttttca agccccgaca atttagctgc tactaaattg tcgggtgcct tttttagttt
    11341 ttattcgttt ttcattctat agaaagcgct tcaataaatc aagacatttt tttgttagaa
    11401 tgctaataat aacaaaaggt tttaactctt cttatgtaca ttacaggtaa aaaaacaaaa
    11461 aaatttctct tttttttgaa ttctgaacaa atagtactgg agtacaaaag acgaggaaga
    11521 gccactatct gctcttcctc gtcttttgat tctcacctgt tattcatgat tctttttctc
    11581 agtttcccga tatttttcca ttttacgact aaaaagctct ccgctacttg gtggcgtata
    11641 tgtgatggca tttgctcctg cttgaatggt ctcagcaata ctttcttccg ttggaccacc
    11701 tgtcgcaatg ataggaagtt caggatattt tttacgaaaa tggcgtactg cttctgcagt
    11761 ttctttacct gcactgatat tgataatcga aataccagct gctaactttt catctatttc
    11821 tgtatatttc gacgttaccg tactgataac tggaatatca actactcgac aaacttcttt
    11881 tactgtctct aaaggcgtag gaccgttcaa aacgacccct aacgaacctt gcgcttctgc
    11941 gaacatgctc atgtaggaag aacgagcacc ttgtgtcaaa ccaccaccta caccggagaa
    12001 aactggaatg tcagctgcct caataatgct tttagtaatc gctggatgag gtgtaaaagg
    12061 atacacagca attactgcat tcgcatttgt attacgtatg atcgcaatat ccgtagtaaa
    12121 gatgatcgaa cggatcaatt ttccaaatat cttgatcccg cttgcttcta aaatgatttc
    12181 cggtactctt acgatatctt ttctcaattc cgtcatgatt tctggcgtaa attgttctgc
    12241 catcagccac ttctcctttg ctatctccgg tcatactgat tcaattgata cgtctcgatg
    12301 acctggatga ttttttgcgc ataaccgggg tctgtcgcat aacctgcatc ttgcaatgct
    12361 tgtgcggctt cctgatagtt ggtggccgta atcacttttt catacagcgc aggattccaa
    12421 tctactcctt ggacgaacag ttgtgtatgg tcatccatgg actgttccca tgaatcatac
    12481 actttgaagt ctccttggat agttatccat tcttcgttaa caaattcctt tgtttccagg
    12541 cttactttct tctgatcccc ataagccttg atcccgaaaa gattattgta tttgctagcc
    12601 aaggtacttt ttccccaatt tgattctaat atagcttgtc ctaaaatgat actaggaagc
    12661 actccataac catcttgtag ttcttgggca tgcggaacaa gccgttcaat aaattgctga
    12721 tgggattgcg cttcattttc ttgctgatta tcagtaaaag gagaagacag attcttcaag
    12781 gaaaaaacaa aggctaagag tatcactgct acaccggcaa ccactgctgg ccaacggata
    12841 ttcttgcgct tttgcttttt ccactttttt ttcgccatga tcaattccta cttttcattt
    12901 aattcattca gatattcttc tagcgtcaat tggtgtttgg tgatatactc agcgttttct
    12961 ttaccgacat aacgtaagtg ccaaggttca tatgtgatct tagttatatc ttcgtgtcct
    13021 tttggataac gaacgataaa tccatactgc gcagcattat ctgcgatcca tttagctcca
    13081 ggctgatctc cgtaacttgc atccaataca gtagaaggat aactgttgta ccagttttga
    13141 tcgacgacat cgactgccag acctgtgtgg tgttcgctgt atcctggctc cgtaatcgtt
    13201 tctttagtaa tctttgtcgc ctcctcttct gatacacctt gacgactcat cacatcttgg
    13261 acattttgtt cgaaaacttg ttgttgggat gatacagacc ggtaagctga gaccatcact
    13321 aatggaaatc cagctgcttc agcagccttg gcaaatgctt catattctgg agcaatgcgt
    13381 ttgtcgatca agtatccgtt acttaatgat gccaattgag tagactcatc aatttctttt
    13441 tctaatttgt gatctggacc aaccaaaact agcttccaat cgtctgtttt aacatctggc
    13501 aattgtttgg acgtacgatc ttttgtttct ttgtccttta ccttgctgct ggttgtttga
    13561 ctgttctttt ctttactagc agttgttttg gtgcttgttc gttcgactgc ttgattgccg
    13621 gcttgaacat gcgaatcatc atcgctaaaa ataacatagc ccattgctgc gatcataatc
    13681 acaactgctc caaatcctaa aaatttattc acttgaaaaa tcctttcgtt gttcttcacg
    13741 ttccccattc gcctcatgaa cgtaagtttc ttccagattt tcattcaccc aatcgagaag
    13801 ttcgattttt tctccttcga tctgtttttg aacatctgct ggcagacgaa aattcaaaat
    13861 atcatcatag ccccattcat catattgaat acttactttc aagtcaaggt atctaccagc
    13921 ctgctgatta tttttcaaag ggatcagctc gacttttgct gctttgctgc taagccattt
    13981 ttgcgcgatg cgtgttttga tttgcttgta ctctgcaacg gcaacgtttc gtttttcggg
    14041 aaaaataatc gtttgacgca aaatcttttg cgttagcatc cattcataat cagtgaaaag
    14101 attttttact ttctgcatcg cttcagatgg cagatttttc tcgccatctt tccatttttg
    14161 ccattgttct ggactgatca gcaagtattc atctaaaaaa tttttttcag tttcaaactg
    14221 ttctaataat acactcataa taagatcaat atatacttga tgcacgttta tcccctcctt
    14281 ttaactattc tacttaagtt ttgatcaaaa agcacctctt aaatcgctgc tttcattttt
    14341 tttaatgttt caagtcctct cctctttatt cttcgtgatt tttgttataa taatagacag
    14401 acaagaaact caatccaact atgaaggagc gttttcagat gaaaaaatat caatggggaa
    14461 tcgttggtct tggtaccatc gcacatgagt ttgccgaaag cttcaatcaa gagacgagcg
    14521 aactaacagc agttgcttcc agaacttcag aaaaagcgga aaacttcgct caccgttaca
    14581 atattccaaa agcttacgga agctatcaag aaatgttaga cgatgctgaa atcgacatcg
    14641 tgtacattgc tgtaccaaat agacagcata tcgaccatat cttggcagca ttggaagcag
    14701 gcaaacacgt tctatgtgaa aaagccatca cgatgaataa aaaagaactg gctgacgcta
    14761 tgcgcttagc agaagaaaag aatttgatct tagcagaagc gatgacaatc ttcaacatgc
    14821 cactttatca gcagttacgc tcaataatgg atacaggaaa actgggggct ttaaaaatga
    14881 tccaagcacc tttcggcagt tacaaagaac cagaccctaa aaaccggttc ttcaatccag
    14941 aattagctgg aggagctctg ttggatatcg gtacgtatgc tgtatctttc gctcgtttct
    15001 ttttgagctc tcaacctgaa gtagttgcct ctacaatggt tccttttgaa acaggcgtgg
    15061 acgaacaatc cgtcactatc ttgcgtaaca aagaaaatga acttgctgct gtcagcttga
    15121 cttttcaggc aaaaatgcca aaagtcggta tcgttgcctt tgagaacggt tatgtgacga
    15181 ttgctgatta cccgcgtgct gatcgtgcgg agatcttatt tacagatggt acaaaggaat
    15241 tcatcgaaag cgggaataca tctgaagcaa tgaattacga aataaagaat atggtacaca
    15301 tgattgaagg tgacttgccc aatcgctctc tcttcttgac aaaagatgtc atcgaaatct
    15361 tggatcaaat gcaagttcta tggaaaaata tgtaatttta aaaggttgtg aaaaagctgt
    15421 tctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcctatttt tcgtttggct
    15481 ggacttatta ccgcagagga aagcttttga acaccgttta agaaagaggt tgtgagccta
    15541 ccgttcagct ccgagtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgttgaat
    15601 attgtgactt gtgaccgagg agttgacagg agaacaccgt ttaaagaaga ggttgtgaaa
    15661 tcagcttttt gacctgaata ttagctgaat tcacaacctc tatttttgga ttttttgttg
    15721 ggagaattct gccttgattt caataaaact tttaaaagac tgcaaaaact ggaacaattc
    15781 tgctcgatcc tcgaattcag ggcgactagt cggcagaggc atttgccgat acaatacata
    15841 aacttcttca atacgtgtta aaatttgtct gccatcattt gactctgaaa atgtttctgc
    15901 agtataaatc aataaaccat agatatgttt tccataataa gctggttgct ggattctctc
    15961 tagattttcc agcatgtctt taatcacatt ggcctgtgct cttctcatag agaagtaagt
    16021 ttcgtagtat agcggctgtc tgagccagta gttttcttga tgttctctgg catcttcttg
    16081 gctttcgcga atgaatgtca gtaaatgttc gcatttgatc tgcacatctt ctttattttc
    16141 tgataaaaga aattctgcca tttgtctcaa aatatttcga aattcttttt cgatctgtat
    16201 ttggttttcc ttcagctgct tcgtacgatc tggcatgtaa acatttgcaa gcaaagcaaa
    16261 tactaatcca atcgccatta atcccgcttc attgccgatc agctgccaag acatgttttc
    16321 ctccactaaa taatgagtga ccaaaaccga atttactact atcccttcgg ttaatttgaa
    16381 gcgagaggca agcgggatga acaggaataa aaaaagactg aagccaaaaa tagtataccc
    16441 aacagcaaaa aaactgaaca gtgctgttgc cgtagctaat ataaaggctg cgattcgatt
    16501 catgcccgta cgcaatgtag agcgcttggt atttcctaca cttaataatg caatgatccc
    16561 tgcagctggc caatacaaca gcgataagct acccgctgtg accatcgaaa agaagacact
    16621 gatcactgtt ttgactgttc gcattcctat acgcatttac ttcccccttt tttattttaa
    16681 ttatacttga ataatgaaaa actagctatc agacaaaagt ttaggtatga tagtaaaaag
    16741 accaaaaagg agaatctcta tgcaatttgg ccgttttcga ctaggtatgc gtacgatcaa
    16801 aagtgcctta gctgtttttt tatgtatctt gttttttcat ttgactcaac gaggaatccc
    16861 gatgattgct gcattatctg ctgttttttc tttgcgtcag gacttgacta caaccgtttc
    16921 ttttggaaag tctaggatca tcggcaatac attaggtggt acattagcta tcatttattt
    16981 ttttgtcaaa agctattttc acaatgattt tttggtagaa ttgatcttgc ttcctattct
    17041 agttattatt gtgatcgtcg tgtcagatgg tattaacaat aattccggta tcatttccgc
    17101 aatagctacg ttgttgctga ttgcgttgag catccctcaa ggcgaatccg ctttgtatgc
    17161 tatccaacgt gtactagata cttttatagg gacattcatt gcaatcggta tcaatttctt
    17221 tcttcgtcct cctgaaacag aaaagaagga agaactatca gaagatttgg tcgaactcca
    17281 aaagaaagaa cgattgctaa gaacaaattt acaagagata gaagctaaga tccaaaaaca
    17341 aaagaaaaat agcgaggact aattttcttt tgcaataaaa taaaaaaatg gagagaagaa
    17401 ctttaaaaag tcttcctcca tttttgattg aacggtgaag tgtactactt tattcttcct
    17461 tcatcgagtt ttgttctgcc tcttttggcg cttcatacat atttacttgg ctagtagtcc
    17521 cacgtgtcga caaattctct acgatttcct tcaagtcaag tcctgttgtc gcttttagtg
    17581 tttcttgagt agaagaaagc aaatttgtgg cataatttgt cactcggttc gctccactgg
    17641 tttctcctga actgcttcct gtatcgacaa cagagatttt ttcgatattt cctagcggct
    17701 gtgctgcttc gcgcatcaat tgcggtagca tatcgataac catactcaag acagctgctt
    17761 cgccatattc cttgaaggca ttggcgatct tttgttttgc ttcggcttct gcttttcctt
    17821 ttgctagtgc tgcttcggct tctgcttgtc cagctaaacg tgtcttattg gcttccgctt
    17881 ctgccagtgc ttccacacgg aatcgttctg cttcggcttc agtcacttct ttgacttttt
    17941 gagcttgtgc ttcttgttct ttagcgtacc gatcggcatc tgcttttttc ttcacttccg
    18001 aatcatattg cttttcacga cgtgtgattt ctttttcttc cagttcgatt tgtttttgac
    18061 gttctacgat tttaatctgc atttcctgtt cgattacttg ttgttgtgct cgggcacttt
    18121 ctaaattgta ggcttggtct gcttttgctt tagcaacatc ttgttcttgt ttgtaggttg
    18181 ctaatttcag ttctttttct tttaatgatt ctgcgatctc tgtttgtcgc tgtaattcag
    18241 ctgcttgtga ttctttttct gcttgagctt ttttgatacg cgtttctttt agagcttccg
    18301 cttctgctat atctgcatct cttttgactt gggcgattct tggtttccct aaagagtcaa
    18361 gataaccgtt tttgtctctg acctctttga tagtgaaaga aacgatgatc aaccccattt
    18421 ttgcaaggtc tacagaagcg acttcttgta cgctttgact gaacttatcg cgattttgat
    18481 agatttcttc tacagtcata gaaccaagaa tcgagcgcaa gtgtccttct aatacttctc
    18541 ttgcttcatt ttccagttct tctcttgtct tgcctaaaaa ctgttctgct gctgtagcaa
    18601 tctcttctac agatgaaccg attttgatga tcgaagtacc atcacacatc accggtacgc
    18661 cttgttccgt atacacttca ggggtggaga cgtccaattt actcgataat agactgatcc
    18721 gattcgaacg ttgaaaaact ggcaagacga atgcgccacc tccacgcaca attttgattt
    18781 tgttattgct ttcatcaatg tgcacatttt tgcttcctaa ataacttcca ctgatgatca
    18841 acgcttcatc cggttttgct gtctggtact tcgctacaaa gacgatcagc aacatcagta
    18901 aaatcaaagc tgcgattgcg atcgcgataa aaacaactgg tatttccatt taacacacgc
    18961 tcctttaagc ttttatcatg ctttcataag gcatgacata acagattctt tccttgatct
    19021 caatgatcaa tactttcgtt ccccgctcag ctgtctgttc ttgggggctg taaaaacaag
    19081 ctggacgact aatggaaccg gtaacacttt tcaactgaat ttctcccata cctcgtacag
    19141 ggatcggtgt gatcacaaca gctacttgtc cttccagagt tttttcagaa gacgacatcg
    19201 ttgattcggc atgtttcatc ggcatcaata tatagaaatt aagcaaaaat accaacacta
    19261 tcgatatgcc tccactagca ataaataaaa ataaagatga ttgatctgtt aaacgctcac
    19321 ctagaaaacc aaataagctt gtgaaagtta accaagggat aataagcatc ggatcaagcg
    19381 ggccatcaaa atcgaataca tctccaaaaa taatcaatag gactgccaat cctgcacaaa
    19441 tcagcaatgt ataccaataa atcgtctcaa gtgcgattcc ccctatcata tacacgcctc
    19501 cttccatttc tacgtctatc ataccaatgt ttgaaaattc tgtatagctt cttttaccga
    19561 ctcagtggtg ttttttatag gaaggttgtg tttatcgttg acaatttgtt aaatttttgt
    19621 tttcatgtta aaattttcac aataaattct ttcgaggtag cctatgtttc ctttttttaa
    19681 gaaaaaacca ttaaagctcc agaacacgat catccttgtc gtttgtttct ccacattcgt
    19741 ttctttgatt gtttctgcca tattgatcag gaattttatt ctttcgatga accgatctag
    19801 tgtgtcagat aaaatctcta gcattgctaa aatcgttgcc aatgataaac tcatccaaaa
    19861 aaagctgcag actggtgacc cgacacatgc gattcaagga ataacaaatg aaatcatgat
    19921 tgcatcgaat ttagatttta tcgttgtcat ggaccctgaa ggcattcgat attctcacgc
    19981 agacccaagt tacatcggtc ttcctttctc aagtcatgaa gatgtgaaaa aagtgattgg
    20041 aggacaagaa tttttctctg aaaaagaagg aatgttaggg ttcggcttcc gctatttcgt
    20101 ccctatttat tatgatcaag agattattgg tgcagtttgt gttggcttaa caaacaacac
    20161 gattgacaat caagtcaaag atggacaaaa aatgattgtt ttttcattga ttattggact
    20221 tagcgtaggc atcataggcg ccgtttttct tgcccaaaaa atgaaaaaag tactgcttgg
    20281 acttgaacca caagaaatag cagaacagtt aagtgaaaaa acgattatcg aaaatgaagt
    20341 agatgaagga atcttagcca tctcttctga taaaactatc ctcttgatca atacacctgc
    20401 aaaaaacatt ttctaccaaa cagagcagct attggatttc gaagtaggaa aaaaatcagt
    20461 gataatctat atgacgtttt gtttaaagag acgattgagc atcaaacgac cgttcggact
    20521 caatccatgt atttaaatgg gatagaaact atttctagcg ttacacctct ttttagcaat
    20581 gaccgttttt taggagcagt cgcaacttta cgaaatcgat cagaagtaat gaaattaatg
    20641 gaggagcttg gggggacgaa acaatatata aactccatga gagctcaaac ccatgaattc
    20701 atgaataaac ttcatgtcat ttctggtttg attgatttga agaagtatga agaagtcacc
    20761 cattttatcc agcagctaaa tcaaaattat aaaaaagaga ttggccaagt ctcaagtctg
    20821 atcaaagtac ctgcaattgc cggtttttta ttaggcaaaa taaacgaagc caaagaacaa
    20881 tctgttgaaa tgctgttaga aagcgattct tttatcccta aaatggagat ggatgactcc
    20941 atccattcac ttttgcaagt gttagggaac ttacttgata atgcaaaaga agccgtgcta
    21001 caaaaacatg aaagaaacgg acaggtccgg attcatttaa actatgacac agaagctgaa
    21061 gtattcatcg tgaccgtctg cgatgacggt gtcgggatat cagaagaaat ctcagaaaaa
    21121 atatttacgc taggtttttc gacgaaggga atcaatcgtg ggtttggatt gaatctcatc
    21181 cattcaatcg tcaaacgcca cggtggattt ttagaatttt ccaaacaaac aaatggcggt
    21241 acaatcgtct atctagaatt accaaaaaag gaagatcaaa atgatgacaa atgtcctgat
    21301 aatcgaagat gatccaatgg ttgcagaatt gaatcgtact tttgtggaaa gcatccctaa
    21361 tatgaacgta atcgaaagtt tcagaaatgt agaagaagga aaaaaatatt tcagacaaaa
    21421 aaacgcagat ctgctgctgc ttgatgtata catgcctggc gaaagtggta tccatttttt
    21481 atcttggctt agacagcaag gaaatgatat cccagtgatt ttgatcactg ctgcagatga
    21541 tatcgaaacc ataaagaatg tgatccgcta cggtgcctta gattatttga tcaaaccgtt
    21601 cactttgcaa cgttttcagc tggcgatcga aaagtatctt gctttctctc agacaatcca
    21661 aacgactgaa aaagccgatc agaaattgat cgatcaagta ttcaaccaca ccactcccat
    21721 tcacgaaata ccatcattca tcgatttgcc taaaggtctg tcaaaactga ctttaaaaaa
    21781 aataagtgca tttattctaa aagaagatac tattttttca acaaaagaac tggctcaaaa
    21841 aagctcactg tcacgtatat ccgtcaagaa atatttgaat tttttagtag aacaagatat
    21901 attgaacgaa agaatcaaat atttggaaat cggccggccg ctaacctgtt atgaattgac
    21961 aaagaatggg cgaaaaaagc tcacagatta ctgttgagtc atcgaattat cccatacaaa
    22021 atagtccaag tatacagaag taacaactgt ttacttggac tatttttaaa cattttattg
    22081 acgaatatcc atgttttctt atcttaattg acgggcttta ggaagagata cctcattttt
    22141 cgtttttcca gtttctatta tttcacgaaa gttctcgaaa cttgtcgaaa tcatatttga
    22201 taatgcctca tctgtatttg aacctacatg cggggttaca agaactctag ggtacatttc
    22261 tactaattgc tggatagcag ggtctggaat cgtctcccat ggcttgaagg atcggaaaaa
    22321 aacctctgtt tcatttgcaa aaacatcggt tgcaaaacca gctaaatgat tgcttttcaa
    22381 agctcttaaa atagcttcat tatcttgaag ctctccacgg gctgtattaa tcaagatgct
    22441 accttttttc atttgagcta aaaatccttc attgatcatt tgatcgtttt cacctgggat
    22501 atgaggtaca tgaatactga caatatcgct tttggctaat aactcttcaa gcgagtggaa
    22561 cgtcaatact tttttagcag cttctgtctg atgaatatcg tagccgatca ctttggctcc
    22621 tagtccctta aacaaaacag cttccgtcaa ccctatcctg cctgttccta tgatccctac
    22681 tgtgcaattt ctgatttcac gactaaacat ttgctcatcc actcgaaaat ctttgaaaga
    22741 tgttcggatc gtcgtatacg cagtatttct caaaagcatc atggcaaaag tcaaagataa
    22801 ctctgctatc gcatttggcg aatacgatgg tacacgggct actgtcatac caaaatcagc
    22861 tgccgcttgt agatcgatat gattgactcc cacagtccgt gtaaaaacat aacgaatccc
    22921 atattcctgc atttttgcaa gattggtctc atctgctata caatttcccc tcaacaatac
    22981 agcatcatgg tcctttgctg acacgatatt gtcatgtgtc aacagttctt cataaagact
    23041 gagatcataa tggtaaatat tcaatttttc aaaaaaatct atctcgttcg gacgaacacc
    23101 gtaacatgcg attctcatac tttcttcctc catttcttat gaaaataaac ctatgccatg
    23161 aatcatttgt aatacaacga tccatacagg tgtcagtacc acagcaaaaa tagttgacag
    23221 taaggatgca ttggaggcta aaagtgcctc cctatcaaaa ctgatagcat aggctgctgc
    23281 cactgttgca gctggtgtag ccatcatgat aacgattgtt gccagtgctt caaaattgac
    23341 tggtagaata ttcgttgccg tcaaaactgc tagaatcacg atattcaatg caggtacaaa
    23401 taccactttg actagactgt aataccatga agttttttca gatgcagctt ttttgaagct
    23461 tacttcacct aatgttgcgc cgattgctag ccacgccaat ggtgaactta aaccagctaa
    23521 ataatccatc ggctgaaata gccacggtgc agtctggtca attcgcaaaa acgctacttg
    23581 gcttatctct ccttctgcac ctgtaactga cacttgtggc agataccctt ggaacagcca
    23641 aatgaaaagg ccggcaaaag tagcgataat gattggattg acgaacatcg ctttgatatt
    23701 tttagcttcc atcttcaagc cactcatttt tatgtatcca taagaataca aaaagattcg
    23761 gtagccaata ttgaaaattg aggaatacat cacgccgaca ggaccataaa tcgcactgac
    23821 gataggcgtt ccgaaaaagg tcgtagaacc aaagatcgtc aggacacgca acgttgtttg
    23881 cttatcccct ttatagctta agaaaaaagg ttttgaaact agaataagaa taatataaat
    23941 aactaatccc caaatcaaga cattgattcc ttgcatcagt aattctgtat tgatatcttg
    24001 catgaaagct cgaaatgcca gtgctggcaa agccactgtt aagacgactt tcgatagaat
    24061 ttttccaaca gattcagaaa aaatcccctt ttttctacaa acaaacccta ataatataat
    24121 aaaaactgtt gaagtgattg cactgacaat cgacatatct gtcaaagtcg atcgaaaaat
    24181 gtcaaataag ttcatctttc tcctcctaat tatgttcatt tattcacaat aaaaaaagct
    24241 tttattttgc taatacgtat taacattttt aattttatct ttacattgtt acttattgaa
    24301 caataggaaa ttaaagaaac ttttgaaact tatgtaaagt gatttttact agaacacaa
//
LOCUS       DAZKSU010000040        23831 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000040 DAZKSU010000000
VERSION     DAZKSU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.040"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8Z3_001633"
     CDS             complement(53..787)
                     /locus_tag="ACG8Z3_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0823947.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1526
                     /locus_tag="ACG8Z3_001634"
                     /pseudo
     CDS             1114..1526
                     /locus_tag="ACG8Z3_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
     gene            1519..2220
                     /locus_tag="ACG8Z3_001635"
     CDS             1519..2220
                     /locus_tag="ACG8Z3_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0823948.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG8Z3_001636"
     CDS             2220..3806
                     /locus_tag="ACG8Z3_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0823949.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISPMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG8Z3_001637"
     CDS             3825..4046
                     /locus_tag="ACG8Z3_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0823950.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG8Z3_001638"
     CDS             4043..4804
                     /locus_tag="ACG8Z3_001638"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0823951.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG8Z3_001639"
     CDS             5184..5693
                     /locus_tag="ACG8Z3_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0823952.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG8Z3_001640"
     CDS             complement(5768..6307)
                     /locus_tag="ACG8Z3_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0823953.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8Z3_001641"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG8Z3_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0823954.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG8Z3_001642"
     CDS             7320..8171
                     /locus_tag="ACG8Z3_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0823955.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG8Z3_001643"
     CDS             8199..8834
                     /locus_tag="ACG8Z3_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0823956.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG8Z3_001644"
     CDS             8854..9627
                     /locus_tag="ACG8Z3_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0823957.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG8Z3_001645"
     CDS             10035..10832
                     /locus_tag="ACG8Z3_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0823958.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG8Z3_001646"
     CDS             10810..11223
                     /locus_tag="ACG8Z3_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0823959.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8Z3_001647"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG8Z3_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0823960.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8Z3_001648"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG8Z3_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0823961.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG8Z3_001649"
     CDS             16000..16560
                     /locus_tag="ACG8Z3_001649"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0823962.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8Z3_001650"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG8Z3_001650"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0823963.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG8Z3_001651"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG8Z3_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0823964.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8Z3_001652"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG8Z3_001652"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0823965.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8Z3_001653"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG8Z3_001653"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0823966.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8Z3_001654"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG8Z3_001654"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0823967.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG8Z3_001655"
     CDS             21907..22485
                     /locus_tag="ACG8Z3_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0823968.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG8Z3_001656"
     CDS             22537..23019
                     /locus_tag="ACG8Z3_001656"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0823969.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG8Z3_001657"
     CDS             23136..23510
                     /locus_tag="ACG8Z3_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823970.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(23623..>23831)
                     /locus_tag="ACG8Z3_001658"
     CDS             complement(23623..>23831)
                     /locus_tag="ACG8Z3_001658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823971.1"
                     /translation="NLTTFENFRHKIFTTNDYYISIPTNNPNNTFSFLTPYPASSPAS
                     RTFFYQKNLYHPIDSTNQPNAVML"
BASE COUNT         7780 a         4286 c         5117 g         6648 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct ccaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DAZKSU010000041        22984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000041 DAZKSU010000000
VERSION     DAZKSU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.041"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(641..1345)
                     /locus_tag="ACG8Z3_001659"
     CDS             complement(641..1345)
                     /locus_tag="ACG8Z3_001659"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0823972.1"
                     /translation="MIFDKVKEQIRKVYITTDRKSELLPSERDLAQEFSVSRPTIRKA
                     LASLEDEGIISKNEGRGYLVKPASEKYVDHELDSFIGFFQDAVQQGKKISSKIIQQTI
                     IPADSTISECLEIEQNTLVLVLERIRYIDDYPTCIAKSCIPLSVLPEIVEHDFAKESL
                     FSVLESRGIRLAYAKRSIEVTPPTDLENLYLDLEKNEPVFVFTSIGFAKNQKPFEYEI
                     SKYPAYKTKFESTVSK"
     gene            complement(1360..2229)
                     /locus_tag="ACG8Z3_001660"
     CDS             complement(1360..2229)
                     /locus_tag="ACG8Z3_001660"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0823973.1"
                     /translation="MKIAVICSGGDSPGMNSALLAIKKTIDSEPDSEVVFFKNGYAGI
                     FKECIASVSLKEVEVFKAGTLIGTARFEKMRDLESRKIIIENLNKMEIDNLIVIGGNG
                     SLKGAELLKNESELQVSVIPGTIDNDFPNTDYTLGFDSALNTIIRSIDDLTSTANAHD
                     RIIIMEVMGRGCFALPEYASIANNTYWVERDDKEYLHLICKKFESEFYSPILLLPEGI
                     SNKQKIIEFITKNLPKETKYLNLAYLQRGSSPTFYDRYIGYLWGQLAAKELIEGKSGI
                     LSQKNGNIMLIPW"
     gene            complement(2337..3353)
                     /locus_tag="ACG8Z3_001661"
     CDS             complement(2337..3353)
                     /locus_tag="ACG8Z3_001661"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0823974.1"
                     /translation="MFNFDQERFLKTQAGAVATNEQISNILESIASDLEEIYFIGSGG
                     VGILFDSTIEFARTRSTFPFHRVIAAEFMIHPNKTFSNKSLVVIPSLSGTTKETLELV
                     EYCNEMNVKTLALVGSPKTPLEKAATYSIYNDVLDDTSSENYYLQGLLIALFLMKQRN
                     EIDINQYNTYIEDLKKIPEALVHAKEQAEPMAKSFAKKHKDTSYHILTGAGDSFAEAY
                     YYGMCILEEMQWIKTRPVHAADFFHGTLELIEAGVSVIITEGEDDSRPLTERVKNFVP
                     KFTDVHVTVDTKDYDLPGISEDTRKIISPVVLATVFERISAFLEEERNHPLTTRRYYK
                     QMEY"
     gene            3543..6266
                     /locus_tag="ACG8Z3_001662"
     CDS             3543..6266
                     /locus_tag="ACG8Z3_001662"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0823975.1"
                     /translation="MKTKILKELNDLLNSYDFPQYFPEECNAQYIAQKFQIKRNTASK
                     HLNELMEQKKVVKINGRPVLFFPVHTIERYISHSLDSFEFSSIDSFREKISKKKADHF
                     FDDLIGAKQSLKEAVQQGISAMKYPGGLPIMYFGSSGVGKSYLVEKLAEYCRQTGIIH
                     ENAPFIELNCAQYFNNPELLTSQLFGHMKGSFTGADSDHIGIIEAADGGIVFLDEIHR
                     LPPEGQEKLFIHMDKGVFRRVGDSGPWRLSHVRYIFATTEQEENFFLETFRRRIPIVC
                     TLPNYQDREISERKEIVFHLLEKESTLINKEILVSEAALSFLIRLDLYGNIGELDGLI
                     KQTIANKLFSGSNKKYIEIHISDLPSRYLLTENIELKNQIASQRFYIFSNGNYVHDCD
                     ETSTVTTNLRGLIHRVSKYYSECHDVNRNSEEFRRFVKDQMAEFNDRFIYQKNSEVLV
                     KYYLPEIQNIVQKISPSLFVHLSGNTVQAIATYLAIRDQWGYGKENASANEIEKLYEN
                     LKCNYLTDLNVFLLIFENALDTTLTIWDKLYISLCVISETGNEAKDINAIILAHGYAT
                     ASSIANVANRLVNQFVIDSIDMPLDISFSEVIDRLLHYIEYRKPKDGLVIFVDMGSLA
                     QIKTEIEQVIEVPTMIINNVTTEMAIETAQLIQSTSDIQKVVKKLPYSQFEKQVLYPI
                     KIRKRTIVVSCNTGLGTSIKIKEMMENNLSKDLGIEFLPYENETLRDTQQLEFLIKTK
                     DIIAIIGTDDPRVSNIPYISLEELFDGEIGKLYKVLLRVYGKEIAEAINQNIVKNSSL
                     NRLIDSLTILDAEKVVNQIEESLKDYQMRVGKTLSSLSKINLYVHISCMIERLIRNQG
                     VSSYPAIDQFLKRYETEVASIQKALSKIEKLYNIKIPMEEIAYVCNIIVSY"
     gene            6394..6816
                     /locus_tag="ACG8Z3_001663"
     CDS             6394..6816
                     /locus_tag="ACG8Z3_001663"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0823976.1"
                     /translation="MRQILCMSHGPLAKGMIETLSIIVGDLDNIDFRCAYVDGNNDVE
                     MIIDDYLKESMGNETIVVTDIFGGSINNEWLRRLPDLSKVYLISGMNLSLLVDLYLKF
                     QLVSSDQIEEIIDHAIQASSGSIKFCNQLDYEQTVDDF"
     gene            6834..7304
                     /locus_tag="ACG8Z3_001664"
     CDS             6834..7304
                     /locus_tag="ACG8Z3_001664"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0823977.1"
                     /translation="MIKLVRIDHRLIHGQVALTWTNAISADCILVANDEILNDPIQKT
                     TLKLAAPHNVKVVIKSIQDSIDAINSGKTDKYKLFVVTKTISDAKRLANGVEQIKSVN
                     IGSVKETSGSSALTHTIFVTEEERAELKGMVSEGIEVFAQQVPTDKAIDLKKSL"
     gene            7328..8110
                     /locus_tag="ACG8Z3_001665"
     CDS             7328..8110
                     /locus_tag="ACG8Z3_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0823978.1"
                     /translation="MDSLLVQAILLGLLTAFIVIDWMTGTNNLSRPIVSSVFVGLIMG
                     DLKMGIMMGATLELAFIGAITIGAARPPDTVSGGILGTAFAIATGKGAEFALTLAFPI
                     AALFLLVDNLLTLLVLPMIARKADRYAEKGELKNVSKMHVLGFILVKSLPRALFVGFS
                     FYLGSPVMERILNAIPTFVQDGINIAAGIMPALGFAILLQMLLKKDVFVYFVIGFALF
                     SYLHVPILGIAIFGACLAYIIVNMETKAAMGSRGEEMVEDDF"
     gene            8100..8924
                     /locus_tag="ACG8Z3_001666"
     CDS             8100..8924
                     /locus_tag="ACG8Z3_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0823979.1"
                     /translation="MTSSGKALTKDDLTKICIRSCALEASWNFERQQHMGFAFAMMPA
                     LEKIYSGNEEEKQKAAQRHLEFFNTAPPLSTFIMGIVASMEEENAKNPDDFDTSTITS
                     IKTALMGPLAGIGDSMFWGTLLVIGIGVGASLAKEGNILGPFLFLLIFNVPNFLLRYF
                     GVMQGYNFGTKLLENIESSGVMKTLTHAASILGLMVIGGMSGSMVSIKVAGSIGQGDE
                     ATKIQSIIDGILPNLLSVLCVFLIYWMLKKGFKTTYVLFGIFIFGIIAAFFGFLTT"
     gene            8959..9957
                     /locus_tag="ACG8Z3_001667"
     CDS             8959..9957
                     /locus_tag="ACG8Z3_001667"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0823980.1"
                     /translation="MLNYNAKEMKKKGKRIYGIREDVEKIAKQAYDSGISNIFFTASG
                     GSVAVMQPFEYVLMTESSIPVYSMTAAELVLMGHKQLNEKSLVILYSKSGDTKETIKA
                     AEYLKERNVPTVGIVSKEDTPLEALSTYAINYLDGRPQEMPLYFLIMSIMNLNGEFPE
                     YTRFADELKNLPTALNSVREQADEKGKKYAEEFKEDPYQIWIGSGNLWGITYSYSMCV
                     LEESQWLRTKSVSSPEFFHGTLELVEKGVCVTLLKTEGETRLLDARVEKFAVQYTDKF
                     NVFDTKDYELPGISDEFRKYFCAPVMWAALGRVSVHLEDIRNHSLDKRRYYRVVEY"
     gene            9974..10828
                     /locus_tag="ACG8Z3_001668"
     CDS             9974..10828
                     /locus_tag="ACG8Z3_001668"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0823981.1"
                     /translation="MTAYVIGFGDNVVDYYENLNQKFPGGNAVNFAVNAKKSGVKAYY
                     VGSLSSDPDGVLLQHAISSEGVDISFSEVIDEPTEQAHVTIKDGDREFIGGVRGSRLT
                     PALSAELIDLMKNSSLVHTSCHSRTETKIKELKKYDVKVSFDFSDLDKYRTHEYLSDV
                     CPSIYIAQFSVATDDQEEINRLISECKDFEVPYLLFTRGSKSPVFVDTRENKTYEGFI
                     RKVESPHDTMGAGDAYFAAFASNFVLSKREKSMIEHVIDCFHKGANLAYETLMIDGSF
                     GHATPINP"
     gene            complement(11402..12652)
                     /locus_tag="ACG8Z3_001669"
                     /pseudo
     CDS             complement(11402..12652)
                     /locus_tag="ACG8Z3_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            13169..13360
                     /locus_tag="ACG8Z3_001670"
     CDS             13169..13360
                     /locus_tag="ACG8Z3_001670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823982.1"
                     /translation="MGKYSFEFKSTIVQEYLEGEGGLGYLAKIQGVRSKKGTRMDQSK
                     VRPKNLTFGGRYLYGKISF"
     gene            13341..14752
                     /locus_tag="ACG8Z3_001671"
                     /pseudo
     CDS             13341..14752
                     /locus_tag="ACG8Z3_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14827..16110
                     /locus_tag="ACG8Z3_001672"
                     /pseudo
     CDS             14827..16110
                     /locus_tag="ACG8Z3_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(16326..17138)
                     /locus_tag="ACG8Z3_001673"
     CDS             complement(16326..17138)
                     /locus_tag="ACG8Z3_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017867010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0823983.1"
                     /translation="MGDSKLTKKEINKVFWRSFALQGSESYTDMQGMGYSFSILPALN
                     KIYQGNDEKYYEAVYRNTEFFNTTPHISTFIMGMSLALEEENAKTDQFETETISSIKA
                     ALMGPVAGIGDSFYWGTFRVIAAGIGLSLAAQGNILGPILYFSIYTLLHFLGRYGCLR
                     IGYRMGANAIEKAFENNYLESFSLAASIVGLMSIGAMTSSLVRFTIPAVFKSKGGEIN
                     VQQMLDDIFPGILSLGLTFFVYFLLKKKVKVLWIILAIFVFSIVGVYIGLLG"
     gene            complement(17155..17886)
                     /locus_tag="ACG8Z3_001674"
     CDS             complement(17155..17886)
                     /locus_tag="ACG8Z3_001674"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0823984.1"
                     /translation="MIECILIGIVGYLTTIDERYFGASMMNRPIIVGPVVGLILGDLH
                     QGILIGAALEAMFIGIVTIGAALPPDVGVAGTIATALAIKSGAGADVAVTLAMPFAVL
                     AQGLNMLAFTINSFTVSQGRKAIENRKLSSFQKWHFFPLIVRLPSAVLTFLVVYFGAN
                     ATESFVNIMPEKLMTGFTVASGLLPAVGFAMLIQGMIDKKSVPYFFIGYLLAAYLGVP
                     VVGVAVAAIMVALLVIQTKRNPVNI"
     gene            complement(17903..18358)
                     /locus_tag="ACG8Z3_001675"
     CDS             complement(17903..18358)
                     /locus_tag="ACG8Z3_001675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0823985.1"
                     /translation="MIKLLRIDDRLIHGQVAMAWTKSVSADYIIVVDDASANDKMKQM
                     ILSLAKPVSTGLDIITSADFLSTFEKRKNQNLMIVTSSPKSAFEIVNAIPGEVDSINI
                     GGLRYAEGKEKVNEYVAITSEDKNYLAKLKESGFKLIMQATPTGKAKIY"
     gene            complement(18351..18761)
                     /locus_tag="ACG8Z3_001676"
     CDS             complement(18351..18761)
                     /locus_tag="ACG8Z3_001676"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0823986.1"
                     /translation="MYTIILASHKNLAKGMKETVEYILGPAPFIKVMTAYTDDNYDIE
                     KDTNILLDGTDKVLVITDLLGGSVNNHWMNYVYEKKLTKKITVIAGMTLSLIMELSMN
                     IEDYKLREKISMIIAESQKSIINCSELMEVEDND"
     gene            complement(19292..20251)
                     /locus_tag="ACG8Z3_001677"
                     /pseudo
     CDS             complement(19292..20251)
                     /locus_tag="ACG8Z3_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(21112..21327)
                     /locus_tag="ACG8Z3_001678"
     CDS             complement(21112..21327)
                     /locus_tag="ACG8Z3_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0823987.1"
                     /translation="MKKFIIPCIFLFLSILLFSTSILVGSYLDSSGMLIEPAFFCVPL
                     GYLTLGLAFFTALYSFVKQRLLKKYSA"
     gene            21810..>22984
                     /locus_tag="ACG8Z3_001679"
     CDS             21810..>22984
                     /locus_tag="ACG8Z3_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0823988.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEEDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTEGLLATANNGYLFLDEVHRLSKENQEKLFVFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKSEDYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADILQKDIIVSKEALSLMLSHPFSGNIGKLKNIIKISCADVYSRTREEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNE"
BASE COUNT         7546 a         3736 c         4412 g         7290 t
ORIGIN      
        1 atttataata aaaatattat tcaaataaat aataataact gcccgcatga tgttgacagt
       61 tacaatagca catagataga ctggcaaaga tggttcttaa tctttcactt tggtcagaat
      121 tcttgggtta tccttccagt gaaagtcagc agaaaatcct taatgcttac aaataatttt
      181 tgcgaaatag gttttattgg taaaggactc aatcatgaat atgaattact tttatctggt
      241 aaatttggaa aaattttgca aagaaataaa aattagacaa atttgctaca cataatcaaa
      301 gattaaaaaa gtttttaaaa tatagttgtt tgaatgggga tatgaattag agaaaattgg
      361 ttgattcaac gggtgctatc atttttgata gcgtcatatg tgaagtaagc acacctaatg
      421 aaaaaggttc aggcttgctt tgaagactta ttaacaacta tgtcgactat ccttgactta
      481 gggtagagga gtacctccac acaccttaat tagatgataa tgtttggact gtaggctact
      541 tagcggatta agataaagag gaagtagaaa aagagccttg cggaaagaca ctttatctgt
      601 tatccgcgag gctctttata catttaagca tatttaaaaa ttatttggaa acagtacttt
      661 caaactttgt cttataagca gggtacttag atatttcata ttcaaacggt ttctgatttt
      721 tagcaaatcc aatacttgta aagacaaaga ctggctcatt tttttctaaa tcaagataaa
      781 ggttttctaa atctgttggc ggtgtaactt caattgagcg tttggcataa gctaatctaa
      841 ttcccctact ttctaatact gagaacaggg attctttggc aaaatcatgt tcaacaattt
      901 caggtaaaac agatagggga atgcaacttt ttgcaataca agttggatag tcatcgatgt
      961 atcgaattct ttccaagaca agcactaaag tattctgctc aatctccaaa cattcactaa
     1021 ttgttgaatc agcaggtatg atggtttgtt ggataatctt agaactgatt ttctttcctt
     1081 gctgaaccgc atcttggaag aatccgataa aagagtcgag ttcgtggtca acatattttt
     1141 ctgaagctgg tttcactaga tacccgcgac cttcattttt agaaataatt ccttcgtctt
     1201 ctaaagaagc taaagctttc cgaattgttg gacgactcac cgaaaattcc tgggccaaat
     1261 ctctttccga aggcaataat tcagatttcc gatcagtggt gatatacact tttcgaattt
     1321 gttcttttac tttgtcaaaa atcatagtaa tccatccttt taccaaggta ttaacattat
     1381 attaccattt ttttgactta atatcccaga ttttccttca attaattcct tcgccgctaa
     1441 ttgaccccac aaatagccaa tatatcggtc ataaaaagtt ggagatgatc cacgctgaag
     1501 atacgcaaga tttaagtatt tggtttcttt tggtaaattt ttggtaataa attcaatgat
     1561 tttttgtttg ttagatatac cttctggtaa aagcaatata ggcgagtaga actccgactc
     1621 aaatttttta catattaagt gtaagtattc cttgtcgtct ctctcaaccc aataagtatt
     1681 atttgcaatg gaagcgtact ctggaagagc aaagcagcct cggcccatga cttccattat
     1741 aataatacga tcatgagcat tagcagtaga agttaaatca tcgattgatc ggattatagt
     1801 gtttaaggcg ctatcgaagc caagtgtata atcggtattg gggaaatcgt tatcaatagt
     1861 ccctgggatg acagatactt gtaattctga ttcatttttc aatagttcag ctcctttcaa
     1921 ggaaccgtta cctccaataa caattaagtt gtcgatctcc atcttattta ggttttcaat
     1981 aataattttc cgactttcga gatctctcat tttctcaaat ctggctgttc caataagtgt
     2041 tccagctttg aatacctcaa cttcttttag agaaactgag gcaatacatt ccttaaatat
     2101 tccggcataa ccattcttaa aaaagactac ttccgaatct ggttcgctat caattgtttt
     2161 ttttattgct aataaagctg aattcattcc gggagagtcg ccaccactac agataactgc
     2221 gattttcatt ttagatcctc ctaaaaaagg tgactgcgat tctattctcc cattagagaa
     2281 aatgaagcaa tcaaatgggc aatggagtcg cggtcacctt tttcatattt ttatgattag
     2341 tattccattt gcttataata acggcgagta gttaaagggt ggttacgttc ttcttctaga
     2401 aaagcactaa ttcgttcaaa gacagtagct aagacgactg gggaaataat ttttcgcgtg
     2461 tcttccgaaa tgccaggtaa gtcatagtcc tttgtatcaa ccgttacgtg aacatctgta
     2521 aacttaggaa cgaaattctt cacgcgttct gtgagaggtc gagaatcatc ttctccttct
     2581 gtaatgatga cacttacacc cgcttcaatt aattcaagag ttccatggaa gaagtctgct
     2641 gcatgaacag gacgagtctt aatccattgc atctcttcta aaatacacat gccatagtaa
     2701 tatgcttccg caaagctatc accagcaccg gttaaaatat gatatgaagt atctttgtgt
     2761 ttttttgcaa aagattttgc cattggttcc gcttgctctt tagcgtgcac taaggcttca
     2821 ggtatttttt ttaaatcctc aatataggta ttgtattgat tgatatcgat ttcatttctt
     2881 tgtttcatta agaagagagc aatcagcagt ccttgaaggt aataattttc tgaagatgta
     2941 tcatctaaaa cgtcgttata gatagaatat gttgcagctt tttctagggg cgttttagga
     3001 cttcctacca aagctaatgt tttgacgttc atttcattac agtattcgac taactcaagt
     3061 gtttcttttg ttgtaccaga taaagatggg ataacaacca aagatttgtt agaaaatgtt
     3121 ttattgggat gaatcataaa ttcggcagcg ataacacgat gaaaagggaa agtgctacga
     3181 gttctcgcga attcgattgt tgaatcaaaa agaataccaa ctcctccaga cccaataaag
     3241 taaatttctt ctaagtcaga tgcgatggat tctaaaatat tggaaatttg ttcatttgtt
     3301 gcaacggcac cagcttgagt ctttaaaaat ctttcttgat caaaattaaa cataaaaatc
     3361 tctcctttga ttaccaatgg taatattttg tttgcataaa taaaatacca caggtaataa
     3421 aaagtgtcaa atatttttta ggaaaaatac cataggtatt gttaaagtgg aaagatgttt
     3481 ataaagaata ttgaatttta tagtgaaaat catttattat taagtaaagg aggtgggttc
     3541 atttgaaaac gaaaatttta aaagaattaa atgatctact taacagctat gattttccac
     3601 aatattttcc agaagaatgt aacgcgcaat atattgccca aaaatttcaa ataaaaagaa
     3661 atacggctag taagcatcta aacgaactaa tggaacagaa aaaagttgtg aaaatcaatg
     3721 gtcgtcctgt tctttttttt ccagtccata caattgaacg gtatatatca cattcattag
     3781 atagttttga gttttcttca attgattcgt ttagagaaaa aatttcaaag aaaaaagcag
     3841 atcatttttt tgatgattta attggtgcaa aacaaagttt aaaagaagcg gttcaacaag
     3901 ggatatcagc aatgaaatat ccaggtggat taccaataat gtattttgga tcgtccggag
     3961 ttgggaagag ttacttagtg gaaaaactag ctgagtattg ccgacaaact ggtattattc
     4021 atgagaatgc tccgttcatc gagttaaact gcgctcagta ttttaataat ccagagttat
     4081 taacaagtca gttgtttggc catatgaagg gatcgtttac cggcgccgat tctgaccata
     4141 ttggtataat agaagcagca gacggtggaa tcgtctttct tgacgaaatt catcgtttac
     4201 caccagaagg acaagagaaa ctttttattc atatggataa aggcgttttt cgaagagttg
     4261 gagatagtgg tccttggcgt ttatcacatg ttagatatat ttttgcgaca acggaacagg
     4321 aagaaaattt ttttctagaa acttttcgac gaagaattcc gattgtttgt actttgccta
     4381 attaccaaga tagagaaatt agcgaacgaa aagagattgt cttccattta ttagagaaag
     4441 agtctactct gatcaataaa gaaatattag tttctgaagc cgcccttagt ttcctgattc
     4501 gcttagattt gtatggaaac attggtgagc tggatggact aataaaacaa actattgcga
     4561 ataaactatt tagtggaagc aataaaaaat atatcgaaat tcatatttct gatttaccta
     4621 gcaggtactt gctaacagaa aatattgagt taaaaaacca aattgcgagc caacggttct
     4681 atatctttag taatggtaac tatgttcatg attgtgacga gacttcaact gtaactacaa
     4741 atttacgagg tttgattcat cgtgtttcta aatattattc agagtgtcat gatgtgaata
     4801 gaaatagtga agagtttcga cgttttgtga aggaccaaat ggcagaattt aatgatcgtt
     4861 ttatttacca aaaaaattca gaagtcttag ttaagtatta tttaccggaa atccaaaata
     4921 ttgttcaaaa aatctctccc agtctttttg ttcacctttc tggcaatacg gtccaagcta
     4981 tagctaccta tctcgctatt agggatcaat gggggtatgg gaaagagaat gcttcagcga
     5041 atgaaattga aaagttatat gaaaacctaa agtgtaacta tttgacggat ttaaatgtat
     5101 tcttactgat cttcgagaac gccctggata caacattgac tatttgggat aaactgtata
     5161 tcagtttgtg tgttattagt gagaccggaa atgaagcgaa agacattaac gcaattattt
     5221 tagcccatgg atatgcgaca gctagtagta ttgctaatgt tgccaatcgt ttggttaatc
     5281 agtttgtcat agattctatt gatatgcctc tagatatcag ctttagtgaa gtaattgaca
     5341 gattacttca ctatatagag tatcgaaagc ctaaagatgg actagtgatt ttcgttgata
     5401 tgggaagttt agctcaaatt aagactgaaa ttgaacaagt aatcgaagtt ccaacaatga
     5461 ttattaataa tgttacaacc gaaatggcta ttgaaaccgc acaactgatt caaagtacga
     5521 gtgatatcca aaaggttgta aaaaaattgc cgtattctca gtttgaaaag caagttctat
     5581 atcccatcaa aattagaaaa cgtacaatcg ttgtttcctg caatactggt ttgggtacct
     5641 ccataaaaat taaagaaatg atggaaaata atttgtcgaa agacttgggc attgaatttc
     5701 tgccatacga aaatgaaact cttagagata ctcagcaatt agagtttctc attaaaacaa
     5761 aggatatcat tgccattatc ggaacagatg atccgcgtgt gtcgaacatt ccctatattt
     5821 ctcttgaaga attatttgat ggtgaaattg gcaaactcta taaagttctt ttgagagtct
     5881 atggaaaaga gattgctgag gcaattaatc aaaatattgt taaaaatagc tcattaaacc
     5941 gcttgattga cagtcttact attttagatg ccgaaaaagt tgtcaatcaa attgaggaaa
     6001 gtttaaaaga ttatcaaatg agggtgggga aaacgttatc atcattgtcc aaaattaatc
     6061 tttatgttca tatcagctgt atgattgagc gactaattcg taatcaggga gtaagtagtt
     6121 atccagcaat agatcagttt cttaagcgat acgaaactga ggttgcttct attcaaaagg
     6181 cacttagtaa gattgagaaa ctttataata ttaagattcc tatggaagag attgcatatg
     6241 tgtgcaacat cattgtaagt tattgataaa ataaagtgac actattcaca gcgatggaaa
     6301 tagtgtcact ttattttttg tacgcccttt catttaagtg gtgacagtaa ttggcacagt
     6361 atttgctaat aacaaagcga aaggagttcg ggtatgagac aaatattatg tatgagccac
     6421 ggtcctctag caaaaggaat gattgaaact ttgagtatta tcgtaggaga tttggataat
     6481 attgattttc gatgtgccta tgtcgatgga aataatgacg tagaaatgat catcgatgat
     6541 tatctaaagg aatctatggg aaatgagacg attgttgtaa ccgacatttt tggtggaagc
     6601 attaataatg agtggctaag acggttgcca gatctttcta aagtctattt gatttctgga
     6661 atgaatttaa gtcttttagt ggatttatat ttaaaattcc aattagtttc atctgatcag
     6721 attgaagaaa ttattgatca tgcaattcaa gcatcttctg gttcgattaa gttttgtaat
     6781 cagctagatt atgagcaaac ggttgatgac ttttaaataa aggagggaag agaatgatta
     6841 aattagtgag gatcgatcat cgtttgatac acggtcaagt tgctttaact tggacgaacg
     6901 caatcagtgc ggattgcatt ttagtagcga acgatgaaat tttgaatgat ccaattcaaa
     6961 agacaacttt gaaattagcc gcaccacata acgtgaaggt tgttatcaaa tcaattcaag
     7021 attcgattga tgcaattaat agtgggaaga cagataaata caaactattt gtagttacca
     7081 aaaccatttc cgatgctaaa cggttggcaa atggggttga acaaattaaa tctgtaaata
     7141 tcggtagtgt taaggaaact agtggcagta gcgctttgac gcatacaatt tttgtaacag
     7201 aagaggaacg agcagaactt aaaggaatgg tctcagaggg cattgaagtg ttcgctcaac
     7261 aagttccaac agataaggca atagatttaa aaaaatcttt gtaataaaaa aataaggggg
     7321 taatttcatg gatagtctct tagtacaagc aattttgctt ggtctcttga ctgcgttcat
     7381 cgtaattgac tggatgacag gtacgaataa cttatcaaga ccaatcgtat cttccgtctt
     7441 tgttggttta attatgggcg atcttaagat ggggattatg atgggggcaa cactggagtt
     7501 agcatttatc ggagcaataa caatcggagc ggcacgtccg cctgatacgg tatctggtgg
     7561 gattttaggg actgcgtttg ctattgcaac tggcaaggga gcagaatttg cgttgacctt
     7621 ggcattccca atcgctgcgc tattcttatt agttgataat ctactaacat tgcttgttct
     7681 accaatgatt gctagaaagg cagatagata tgctgaaaag ggcgaactaa aaaatgtgtc
     7741 gaaaatgcat gttttaggtt ttatcttagt aaaaagttta ccacgggctt tatttgtcgg
     7801 attctcattt tacttaggaa gtcctgttat ggaacgaatc ttaaatgcta ttccaacttt
     7861 tgtccaagat gggattaaca ttgctgcagg cattatgcca gcactaggtt ttgctatttt
     7921 attgcaaatg cttcttaaga aagatgtctt tgtatatttc gtaattggtt ttgcactctt
     7981 tagctactta catgtgccga ttctggggat tgcaatcttc ggcgcatgtc tagcttatat
     8041 tatagtaaac atggaaacca aagctgcaat gggttcaaga ggggaggaga tggttgaaga
     8101 tgacttctag tgggaaagct ttaactaaag atgatttaac aaaaatttgt attcggtcat
     8161 gtgcattaga agcatcatgg aattttgagc gccaacaaca tatgggcttt gcttttgcta
     8221 tgatgccagc gttagaaaaa atttatagtg gcaatgaaga agaaaagcaa aaagcagctc
     8281 aaagacatct agagtttttt aacacagctc cgccattatc aacattcata atgggaattg
     8341 tggcttctat ggaagaagaa aatgccaaaa atcctgatga ctttgatact tctacaatca
     8401 cttctattaa aactgcattg atgggaccat tagctggtat tggtgattca atgttttggg
     8461 gtaccttatt agttatcggt atcggtgtag gtgcttcatt agcaaaagaa ggcaatattt
     8521 tgggtccatt tttattttta ctaatcttca atgtccctaa ttttcttctc cgttactttg
     8581 gagttatgca gggatataac tttggtacaa agctattaga gaacattgaa tcctctggcg
     8641 tcatgaaaac gttgactcat gctgcctcaa ttttgggtct tatggtaatt gggggcatgt
     8701 ctggaagtat ggtatcaatc aaagtagctg gttctattgg acaaggtgat gaagcaacaa
     8761 aaattcaaag tatcattgat ggtattttac caaatctgct gtcagtatta tgtgtatttc
     8821 tgatttactg gatgcttaaa aaaggcttta aaacaactta cgtgttattc ggaatcttta
     8881 tctttggtat catagcagcc ttcttcggat ttttgacaac ttaattgaat aatctaaata
     8941 attgtaagga gagaactaat gttaaactat aatgcaaaag aaatgaaaaa aaaaggcaaa
     9001 cggatttacg gtattcgtga agatgtagaa aaaattgcaa agcaagctta tgattctgga
     9061 attagtaata ttttctttac tgcttctggt ggatcggtag ccgtaatgca accatttgag
     9121 tatgtattga tgactgaatc aagtattcct gtttactcta tgactgcagc tgagcttgtc
     9181 ttaatgggac ataaacagtt gaatgaaaaa tctttagtaa tcttatactc aaaatctgga
     9241 gatacgaaag aaactattaa ggcagctgaa tatttaaaag aacgaaacgt gccaaccgtt
     9301 ggtattgtaa gtaaagaaga tacaccgtta gaggctctat caacttatgc aatcaattac
     9361 ctagacggcc gccctcaaga aatgccactt tatttcttaa ttatgtctat catgaatttg
     9421 aatggggaat ttcctgaata cactcgtttt gctgatgaac tgaaaaactt accaacggct
     9481 ctgaattcag ttagagaaca ggctgatgaa aaagggaaga agtatgccga agaatttaaa
     9541 gaagatcctt atcaaatttg gattggtagc gggaatttat ggggaatcac ctactcttat
     9601 tcaatgtgtg tgcttgagga atctcaatgg ttgcgtacaa agtctgtctc atcaccagag
     9661 tttttccatg gtaccctcga gttagtcgaa aaaggagttt gtgtaacact tttgaaaact
     9721 gagggggaaa cacggctttt agatgctcga gttgaaaaat ttgcggtcca atatacagat
     9781 aaatttaatg tttttgatac taaggattat gagctaccag gtattagtga cgaatttcga
     9841 aagtactttt gtgctccagt tatgtgggca gccctaggtc gcgtcagtgt tcatcttgaa
     9901 gatattcgga accactcttt ggataaacgt cgttattacc gagtagttga atactaaaag
     9961 taggtgatta attatgacag cgtatgtgat tggatttggc gataatgttg ttgattacta
    10021 tgagaatttg aatcaaaaat ttccaggggg aaatgcagtt aattttgctg tgaatgcaaa
    10081 aaagagtggt gttaaagcat actatgtcgg tagtctatca tctgatcctg atggcgtgtt
    10141 attacaacac gccatcagtt ctgaaggtgt tgatatctct ttctcggaag ttattgacga
    10201 gcctactgag caagcacatg taactattaa agatggagat agggagttta taggtggcgt
    10261 gcgtggctcc cgattgacac ctgctctctc agcagaactt attgatttga tgaagaacag
    10321 ttcattagtt catactagtt gccatagtcg aacagaaaca aaaattaaag aattaaaaaa
    10381 atatgatgtt aaagtttctt ttgatttttc tgacttagat aaatatagaa cgcatgaata
    10441 cttgtcagat gtttgtccgt caatttatat tgcacagttt tcagtcgcta cagatgatca
    10501 agaggaaatt aatcgcttaa ttagtgaatg caaggacttt gaggttccgt atcttctttt
    10561 tacgcgtggt agtaagagcc ctgtttttgt tgacactcga gagaataaga cttatgaagg
    10621 attcattcgc aaagttgagt cacctcatga tacaatgggc gcaggtgatg catactttgc
    10681 agcttttgca agtaattttg tcttatctaa acgtgaaaaa tcaatgatag aacatgttat
    10741 tgattgtttt cacaaaggtg caaaccttgc atatgaaaca ttgatgattg atggctcctt
    10801 tggtcatgct acacctatta acccttagct ttgtgctttg ggcttttatt gctggaattt
    10861 gtatagtaga ctcatcataa ctatattttt gtatgtgaaa aggcaagagg ctatgatgaa
    10921 aataacactg gctaattgca tgagggagca tatcgttgtt aaagcgtcat tgatagtatg
    10981 aataaatatc tcgataatat tatattgtta tttacttaat ttattgatta gtcaagcaaa
    11041 gataatcttc tgttacttaa cgagactagt aggtacaagc acttcatgtt atgtacgacc
    11101 caaaaatatt gctgttgatt atttgctgaa attattgcga gcttcaattt atttttaaag
    11161 gacttggtag gctgcgtgat gaaataagac ttggaaggct gtctaaacca atatatagta
    11221 gactaaatag taaaaaagag catggtaatt gtctctttgt caataagtct aatggtaata
    11281 agacaagttg gagattgaga atatttttat tgtgtaacct attttgaagc gtacttaact
    11341 agccccggcc aaaatcaaat tttttcgggg tatatcaagg gtttgcgtca ataacagaat
    11401 gttatttatc tatcacgttc cttggtggcg tgtagagttg atggttacgt agtagcgtat
    11461 ctaccagacg cgtaaatttt cttgcggtta agacgtgtgc tcgtttgtgt ttatttttgg
    11521 gacctcttta catttgcttt gataaaaagc tttatattga ggaagatatt tttttacaga
    11581 gttggaagct tcaactaagt agtatctaaa atagcgactc ctttgttttg tcagaggtgt
    11641 attttgtgac cgatagttaa cagattgttt gcctttccaa cataagcctg cgtattttgc
    11701 caattttgtt tgatcttcaa atcgttcaat ttgaccgatt tcagcgatta atccagcggc
    11761 gtgtaccttt ccaatacctg gaacactagt caaatattga tattctggaa taacgacaac
    11821 aaattgctcg gttgctttat ccaagtcttt gatgttcttt tcaagcgcac gaatttcaca
    11881 tacaaaaaca tttagaacga cattgatgga ttcttatgca agactatcta accgataact
    11941 cacggcaatt gcacgttgaa tcgatttggc atttttttct ggatctttaa agcgacattt
    12001 cctttttctt ttagtaagtc acagaaagct tccaaaggaa gcacagccaa ttcatctagc
    12061 gtgaagtctt ctgtcacaag agtaatgaca gtggcactat agagacttgt cgattcatcg
    12121 cgcatttatc gagtaagcgt attgcatttg tacgttagat tttccagaaa gtgttgtttt
    12181 gtacgagtga agttgcttaa tgagtcgata gcgagtgcgt gttaaacgct gtaaagccac
    12241 gtacttctct tctttgatag gagaagttgt gaaccgttga atacgcaata aatcagcaat
    12301 tcttaacgcg ttgtttcgat cagtcgtatc ttcctcaaac atgcgactga attgtttcat
    12361 ccgaaaagga ttttcaatcc tcacaagtgc attgagtttt tttagctctg catcttcatg
    12421 gaaaaacatc gaaggatgaa agctgtacat ggacgtttaa ttccatctca ataacgattt
    12481 gaccgaagtg attgttttca ttgaacacaa gaatcagttc tcgtgtcaat ggagcccttt
    12541 caatgtcatt agcaacagaa ttttcagtca atatagatag ctgatcgtcg tctgttagga
    12601 agcaaacgtc taatttttca gaactaacgt cattaccaac aaataatttc atggaataaa
    12661 aacctatttt cgatataaat tctagaaaat gcttcgatac tgtgggattc cacagaaact
    12721 cactgtgtgg tcacgacctc gcctaataga atacggtaat aaaagagcta atgagttgat
    12781 agtaaacaca gcgctttgga taacagactt atgaaggtag acaaagctac aggactgatt
    12841 agcaaattct cgttgaatcc tttccataca tccattgtac tctgagaaat cacacaataa
    12901 cgaaataaat tccggaagga atatgttatt caaaaaaatt tgtgttttat agatacttta
    12961 aaagaaactt attaggcaaa atatatttta cgaagactga tgagttgtgc aaaatcaaat
    13021 ctgagtcaga atgaacttca ttctagctca gatttttgtt taatgacttc agtcagtttc
    13081 ttgcattacg tgaatgaaaa tgggactggt gatgtccaaa ccctaaaaag ttagaccaaa
    13141 atctaacttc aggggggatg gtatttttat gggaaaatat agctttgaat tcaagtcaac
    13201 aatcgttcaa gagtatcttg aaggagaagg tggattagga tatttagcaa aaatacaagg
    13261 agtgagatca aaaaaaggta cacggatgga tcaatcaaaa gttagaccaa aaaatctaac
    13321 ttttgggggt cgttatttgt atggcaaaat atccttttga attaaaacta aaaatcgtta
    13381 aagaatatct tagtggcgtg ggcgaatatc ggcatcttgc caacaaatac ggtatttcaa
    13441 gcaagtctca agtaagtgat tgggtgaatg cctatcgtga atacggagag gaaggtctat
    13501 tgaaaaagca tcaaaatcag aaatattctg ttcaatttaa gttaaatgcg ctagagttat
    13561 atcaaacaag tgagatgtcc tatcgagaag ttgccagcac tttagaaatg aacaaccctg
    13621 cgcttattgc caattggatg cgtaatttca gagaagcagg tattgagggg ctctcaaaag
    13681 agaaagggcg accacttact ttgactagaa aaaaagaaaa caaaaaagaa aatacaacaa
    13741 ctgaagaacg cgagcgtatc aaggcattag aaaagcaagt ccgatccctt caaattgaga
    13801 atgccttttt aaaagagttg aggaagctgc gaaaacagga agcccagcaa agacgcaaga
    13861 atcaatcgca cgaatcatcg ccagcctccg aggatcattc aaactagttg agcttcttga
    13921 aacattgaaa tttcctaaag caacctatat gtattggcag aaacggtttg acagaccaaa
    13981 cccagaccaa gaaattgaag aaaaaatgct tgaaattcgc aaggagcata aagactatgg
    14041 ctgtctgcga ctaaaaaaag aattagagaa tcaagggatt catgtcaata aaaagaagat
    14101 tcaacgtctg ataaaaaaac taggtatcga agtaaagtct tacactcgaa aatctcgacg
    14161 gtacaattct tatcatggga aggttggcac tgtcgccaaa aatcgtattc atcgtcggtt
    14221 ttatacaaat atctgtcacc aaaaaattac aactgatacc acagagttta agtattatga
    14281 agtagacacc acaggtgtcg ttcgtcagaa aaagctgtat ttagatcctt tcatggattt
    14341 ctacaatagt gaaattctat cttatcggat ttctgaaaaa ccaaacgcac tagccataat
    14401 ggaaggatta gaagaagcaa tccagatgac aaatgattgt ccatttcgta gaaccttcca
    14461 ttcagatcaa ggatgggcct atcaaatgaa tgcttataag aataaattga agcagcataa
    14521 aatcttccag agtatgtctc gtaaagggaa ttgtttagat aactcaccta tggaaaactt
    14581 ctttggttta ttaaagcaag agatttttca tggagaagtc tatcggagtt tagatgaatt
    14641 gaaaacaaaa attgaccaat atatctatta ttacaaccat aaacgcatca aaaagaaatt
    14701 aaattggcga agtccggttc agtttcgaga agctgtaaaa acagctgtct aacagttaat
    14761 tttatcgtac aaaaaaggag tggatttctc cactccataa aagtctaact ttttggggtc
    14821 gctacaaatg cttatcgaga atttggagaa gaaggcctct tgcgtaagcg aaataagcga
    14881 agttattctg ttcaattcaa gatagatgcg atagagttat aaaaaacggg tgagctatcc
    14941 tatcgtgaag tggcgaatca tttagggata aaccaacctg cgttagttgt caattggatg
    15001 aaaatttttg ttcaaaagga ttgcacgggc tctcaaaaat gaaagggtcc tcctccattt
    15061 tgtctaagaa agaataaaag gaagaaacaa gcaaactgac gcctaaagag cataatcgta
    15121 tcaaagaatt ggaaaaataa gtgcgtttcc ttcagattga gaataccttt ttaaaagaat
    15181 tgaggaagct tagaaaacag gaagtctaac aaagacgaac gaatcaatcg cacgaatcat
    15241 cgacaacctc cgaggatcat tcaaattagt tgagcttctt gaaaccttga aatttcctga
    15301 attaaccttt atgtattggc aaagacgatt tgatcgaaga aattcagatc aagaaatcga
    15361 agaggaaatg cttaaaataa gaagtcaata taaagattat ggctatttac gtatgacaaa
    15421 ggaattgtgg gaccgtgggt tccatgttaa caagaaaaaa gtacaacgtt tgattcgaaa
    15481 gctgagtatt gaagtacgat catttacgag aaaatatcgc cgctataata cttatcgtgg
    15541 aagaatcggc atgattacaa aaaatcgaat tcatcgacgc ttttacacaa gcaactgtca
    15601 tcaaaaggtt acaacagata cgacagaact aaaatatttt gaaatggata aatacggtgc
    15661 caatcgtgaa aagaagctgt atcttgatcc atttttagat atgtacaaca gtgaaatttt
    15721 attttatcgc atttctgaaa agccaagtgc gttggctatc atggaagctt tagaggaagc
    15781 tatctgcgca acggaatgta tctatcgtcg aacttttcac tccaaaaagg atggacctac
    15841 caaatgaaag cttatagtca taagttgaaa gaacataaga tcttccaaag tatgtcccgt
    15901 aaggagacct gcttagataa ttcgatcatt gagaacttcc ttagtctatt aaagcaggaa
    15961 atttattatg gaagagacta tcgtatcttt gaagaattga agcaagccat tgatggctat
    16021 attcattact acaaccatga gcgtatgaaa aggaaactaa actggcagag tctggtgcaa
    16081 tttagagtaa tagcattcaa agctgtctaa aatcatcaag aaaagcaaag aaaaacggag
    16141 tgaattttct ccactccgaa aacatctatc ttttttaggt cactacagaa tcaattctga
    16201 ttcatcagac ccataaatta tagatcctaa taatttatac catgctcttt ttactcatat
    16261 ttacatttat ctttgggtat tttatggttt aagttgatgt ctgatctagt tttattctgg
    16321 attaattaac ctaataatcc aatataaact ccaacaatac taaagacgaa gatagcaagt
    16381 ataatccaaa gaacctttac ttttttcttt aatagaaagt aaacaaagaa tgttagcccc
    16441 aatgagagaa ttcctggaaa aatatcatcg agcatttgct gaacatttat ttctccacct
    16501 tttgatttaa atacggcggg aatagtaaag cgtaccagag atgaggtcat ggctccaatt
    16561 gacattaaac ctacgataga tgctgctaac gagaaacttt caaggtaatt gttctcaaat
    16621 gctttttcaa tggcgtttgc tcccatccga tatccaatac gtaagcagcc atatctgcca
    16681 agaaaatgta acagcgtata aattgaaaaa tacaatattg gacctaaaat gtttccttgg
    16741 gcagctaaag ataagccgat gccagcagca ataacgcgaa atgttcccca gtaaaaacta
    16801 tcaccaatac cagctacagg ccccatcaat gcagctttta tcgatgaaat tgtttcagtt
    16861 tcaaattgat ctgtttttgc attttcttct tcaagtgcta atgacatacc cataataaag
    16921 gttgaaatat gaggcgttgt attaaaaaat tccgtatttc ggtaaacagc ttcataatat
    16981 ttttcatcat tcccttgata tattttgttt aaagcaggta atatagaaaa actatatccc
    17041 attccttgca tatcagtgta tgactcggat ccctgtaaag caaatgaacg ccagaaaacc
    17101 ttgtttattt cttttttagt taatttagaa tctcccataa ttatcactct ccctttaaat
    17161 atttacagga ttgcgtttag tttggataac taataaagca accataattg cagcaacagc
    17221 tacaccaact acggggactc ctaaatatgc cgctaaaagg tatccaatga aaaaataagg
    17281 aactgatttt ttgtcaatca ttccttgaat tagcatggca aatccaacag cggggagtaa
    17341 accagaagct acggtgaaac cggtcattaa tttttctggc ataatattta caaaactttc
    17401 tgtagcattg gcaccaaaat atactactaa aaatgtaagt actgcggaag gtaagcgaac
    17461 gattaatgga aaaaaatgcc acttctgaaa agatgataat ttgcggtttt caattgcttt
    17521 tctaccttgt gaaacggtaa aggagttaat cgtaaaagcc aacatgttta atccttgtgc
    17581 caatacagcg aaaggcatcg ccaatgtaac agctacatca gcacctgctc cactttttat
    17641 tgctagagcc gttgcaatag taccagctac acctacatct ggtggtagtg cagcaccaat
    17701 cgttacaatg ccaataaaca ttgcttctaa tgctgctcct atcaagatgc cttgatggag
    17761 atcccctaag ataagtccaa caacaggtcc aactattatc ggtcgattca tcatagatgc
    17821 cccaaaatat ctttcatcta ttgtcgttaa atacccaact atgccaatta aaatgcattc
    17881 aatcattctt attccccacc ttctaataaa tcttcgcttt tccagttgga gtagcctgca
    17941 taattaattt gaagccggac tcctttaatt ttgctaagta gtttttgtct tctgatgtga
    18001 tagccacata ctcgttaact ttctcttttc cctcggcgta ccgtaaacca ccgatattaa
    18061 ttgaatcgac ttcccctggg atagcgttta caatttcaaa tgcgctttta ggggaactag
    18121 tgactatcat caaattttga tttttccttt tttcaaaggt acttaaaaaa tctgctgaag
    18181 taataatatc tagtcccgtt gatacaggtt ttgctaatga taaaatcatt tgtttcattt
    18241 tgtcattagc agaggcgtca tcaactacaa taatgtagtc agcagatacg gacttagtcc
    18301 aagccattgc aacttgaccg tggatcaggc gatcgtcaat tcttaatagt ttaatcatta
    18361 tcttctacct ccattaattc tgaacagttt ataatgctct tttggctttc agcaataatc
    18421 atggaaattt tttctctcaa tttataatct tcaatgttca tacttaattc cataattaag
    18481 gacaaagtca tacctgcaat tactgtaatt ttttttgtta attttttttc gtaaacataa
    18541 ttcatccaat gattatttac gctaccacca agtaaatcag tgatgacaag gactttatca
    18601 gttccatcga gaagaatatt cgtatctttt tcaatatcat agttatcgtc tgtgtatgca
    18661 gtcattactt ttatgaatgg tgcagggcca aggatatatt cgactgtctc tttcattcct
    18721 ttcgctaaat ttttgtggct tgcaagaata attgtgtaca taatccacct ccatttattt
    18781 ataaataata taccattggt aatctaaatg tcaataaatt atttcgtatt aagaataaat
    18841 aaaatacctt tggtaattag tggggcgaat ctaaaaactt taactaaaat tctcttttgt
    18901 aaattttttt gaacgcggct atcgtggtga ttttttattt ctgtttgttt aatgaatttg
    18961 taaattagga ggtgattttt gtgatggtgt aaacaaagcc tataaaaatg aagtaaagca
    19021 ttgtgttttg tccagatgtc aaatctttta tgtatcggtt gtatgtatag tatgttacac
    19081 ccaaaataga taacccaagt ccaaatggag agacagatgt tctttatatt actggcgaag
    19141 acatgaagta ttcttataaa atatctaaaa atgagtataa gtttaaagaa aataatttat
    19201 ctatataaac tattctgctt tgattagcaa gtctttcaat cggtaaaata ttatttgaat
    19261 agatgggata tggtttatta ttttctaaat gtcaaattaa gttagacaat tgctcctcac
    19321 ttatgtaact caaaaatact tctaacgatg ttcgataatt taaagatttc cttggtatat
    19381 gatttctttt ggaggcaacc gccgccacaa atttttggtc aacctgattg aaatccattg
    19441 tattaggaag tccgtcttta cgtaacaatc cattagaatg ttcatttaat ccccgttgtg
    19501 acagcgttcc aggatctgca aaataaatag aaatatcgtt agcattactc atgtttttcc
    19561 aattcgaaaa ttcttttccg caatcaaagg taatggattt gaacaagttt cggggaagct
    19621 tttgtagcca gtcagttgtc actttttcaa tatctagtgc tttccgtcct tcgggtttta
    19681 aagtaataat cacttttgac aagcgctcaa ccagcgtgat gacagcactt ttatgatgga
    19741 cacccacaat agtgtcgcct tccaaatgtc caaattcctg ttgaaattca ggatagtccg
    19801 tgagacgttc tgaaatattc cgtttgaagg cttgtttccc tcgacgttct ttatgcccgt
    19861 tgggtttccg ttttcctttc atgggtagtt tcgagcaatc aaattagttt gttttaaact
    19921 tgcgataaag ggttcgaaca gaacatgaaa ttgaaaactc agcacgaccg ataatcacat
    19981 cgggtgtcca tccttgcgcc accttgtttc ggatatacgc atcttgttct gctggcaaac
    20041 aaatgggtcg tcggccgcac cgtgttttat ttttttgata ttgttggtaa tagtcatgga
    20101 tagattggcc ttgtttcagg aagttgtaca cattgtagat ggtctgtctg gaacgatttg
    20161 aggtcttagc gactacggta acagattgat gcatattgaa ataggcttct atcatcacga
    20221 gttcatttgt tgtaagatgg gtataggtca tttgtcttca ctccttataa ttctttggtc
    20281 ggaactattt tgagtgtagc acaaatgact tttttagttg tctagcttaa ttttacaatc
    20341 tgcgtttaat aaactctcag tgggatagtt tgactccgta tatgactccg gttgatttga
    20401 ttgaattcag atgattatac atactataaa tttctctatt atgttgccat ttgatttgaa
    20461 taatgctgaa agaaggattt tagtctataa cgaacaaggg aataaaacca ataaaaaaag
    20521 atatagaata tcaatataac aaaaagagat tgtgacataa ttctctgaaa aaagctctag
    20581 aaatgacgag tcattcgtca tttctggagc tttttaggta aaataaaaga gtacgagagg
    20641 atactcgcac tctaaatcat tcaacccttg aaaggatcaa aatcatgcat acaaattata
    20701 acatgaatca actcacttta gatatcacaa cttcttattc caaaagaaga tagcaccgct
    20761 gggtttgtca atgaactcgt tgaaaccctt gaaatcaacg agccgtatct ttttggccgc
    20821 cctaggaatt gtgatttgag tgccatgtta aaattggttc ttttcgcata ttgaaaatga
    20881 ttgaagagga ctatcggtat tacaaaagac aatataacca gcaggtgaaa aaatggagaa
    20941 ggtaattccg aaaaaagctt cttttggcga ttgggaataa ttgttggtaa tggaggaata
    21001 aaatgaatgt ttatgcagac gtttgatgtc tacatcaaca tccattctta atactcgaag
    21061 gttcgttgtt gaaaaaacta acttttcttc aacagaggat ttacgtgcaa atcaagccga
    21121 gtatttcttc aataatcttt gtttgacgaa actgtataag gcagtaaaga aagcaagtcc
    21181 taatgttaag tagcccaacg gaacacaaaa gaacgctggt tcaatcagca ttccgcttga
    21241 atcgaggtaa gaaccgacta ggatggaagt tgaaaataac aaaatactca agaaaagaaa
    21301 tatacaagga ataatgaatt ttttcatggt gtctccttct ttgtttttct agattatata
    21361 gaatagaacc taggaaaaga taagtgtgac aaaattgata ggtgtgaaag gcattgagat
    21421 atcaaccagc tttatccttt ggagtatgct attgtgatta atgagacaga ggatgtggga
    21481 ccagacgata ctgaatcaac ggaaattgtt ctaaaccacc atcaaatagt agtttttcta
    21541 gtatttgaag gtgatttttt tatgttcgaa agtaactgta aaaaagataa ataatcggca
    21601 gttattcttt cacataagta ggttggatgc aggtgagaga agtttattgt atgagaggaa
    21661 gattggtaat ctaactatag ttttataact aatatcagtt taagctcgat tatttttgtt
    21721 tcaaagtaaa attggaacag aaatatagaa tagacatatt gtagccgata gtttattatg
    21781 cttctagaca cgttgaactg gaggggaaaa tgaaaaagag cgctaaagat cgagtttttg
    21841 atgagctgat acgactaaca gggaatatgt cttctgtcac tgccgagaaa atttcgaaga
    21901 atctaggtat ctctcgtcag aatgccagcc attatttgac gagattagtg gaagataaaa
    21961 aggtcgagaa attaccggga aagcctgttt tgtggaagcc tttagatgaa tatgcggttg
    22021 tagataatac ggagcaaata aacgaagcct ttcattctgt ggtgggtcat gacggaagtc
    22081 tgagagaggt cattcaaaaa tgtattgcag ctgtcaaata tccgcctaac ggactcagtg
    22141 tattaataaa tggagccacg ggggttggaa aaagtttttt agccacaaaa atttttgaat
    22201 acgctatcca cgagcaaatt attgaagaag atgcaccatt tgctatttta aattgtgccg
    22261 attatgcgga caatcccgaa ctgctttcgg caacactgtt tggatacaag aaaggggcct
    22321 ttacgggggc tgagaaggat acagaggggt tattggcaac agccaacaat ggctatcttt
    22381 ttcttgatga ggtccatcgt ttgtcgaaag aaaatcaaga aaagctcttt gtttttattg
    22441 atactggaaa ttatcgtcca gtcggtgaaa atgtaaactg gcattctgct aaggttcgtt
    22501 ttatcttcgc cacgacggaa aaaagtgaag attatttatt agacaccttt gatcgacgta
    22561 ttcaaatcag tgttatgttg ccaacttttg aagatcgtcc cattcgagaa cggttagagc
    22621 tgattcaatt attttttcaa aatgaagcgg atattttaca aaaagatatt attgtttcaa
    22681 aagaagcttt gtctctaatg ttatctcatc cctttagtgg gaatattggt aagctgaaga
    22741 atatcattaa aatcagttgt gcggatgtgt acagtcgaac acgggaagag ccattaacga
    22801 ttgggaaaaa tgagatcttg attcagttaa atatgctgga atccgaagta gaaagcttgc
    22861 cgattgctag tttattgata aagaaaaatc aaaagataaa aacggatcga atcaaaccgt
    22921 ttgattatga ttcgattttg gaaaaaatta ctcagctgat aaatgaaggt aatttcaatg
    22981 aaaa
//
LOCUS       DAZKSU010000042        22833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000042 DAZKSU010000000
VERSION     DAZKSU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.042"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1440)
                     /gene="brnQ"
                     /locus_tag="ACG8Z3_001680"
     CDS             complement(61..1440)
                     /gene="brnQ"
                     /locus_tag="ACG8Z3_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0823989.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            2146..3117
                     /locus_tag="ACG8Z3_001681"
     CDS             2146..3117
                     /locus_tag="ACG8Z3_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0823990.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            3141..5333
                     /locus_tag="ACG8Z3_001682"
     CDS             3141..5333
                     /locus_tag="ACG8Z3_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0823991.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEVQEE"
     gene            5350..5826
                     /gene="ybeY"
                     /locus_tag="ACG8Z3_001683"
     CDS             5350..5826
                     /gene="ybeY"
                     /locus_tag="ACG8Z3_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0823992.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            5810..6205
                     /locus_tag="ACG8Z3_001684"
     CDS             5810..6205
                     /locus_tag="ACG8Z3_001684"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0823993.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            6220..7119
                     /gene="era"
                     /locus_tag="ACG8Z3_001685"
     CDS             6220..7119
                     /gene="era"
                     /locus_tag="ACG8Z3_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0823994.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            7262..8074
                     /gene="recO"
                     /locus_tag="ACG8Z3_001686"
     CDS             7262..8074
                     /gene="recO"
                     /locus_tag="ACG8Z3_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0823995.1"
                     /translation="MSQLVESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            8458..9375
                     /gene="glyQ"
                     /locus_tag="ACG8Z3_001687"
     CDS             8458..9375
                     /gene="glyQ"
                     /locus_tag="ACG8Z3_001687"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0823996.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            9377..11452
                     /gene="glyS"
                     /locus_tag="ACG8Z3_001688"
     CDS             9377..11452
                     /gene="glyS"
                     /locus_tag="ACG8Z3_001688"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0823997.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRVSRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQITAMALSL
                     ASLDQIITK"
     gene            11836..12615
                     /locus_tag="ACG8Z3_001689"
     CDS             11836..12615
                     /locus_tag="ACG8Z3_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0823998.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            12656..13600
                     /locus_tag="ACG8Z3_001690"
     CDS             12656..13600
                     /locus_tag="ACG8Z3_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0823999.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            13616..14395
                     /locus_tag="ACG8Z3_001691"
     CDS             13616..14395
                     /locus_tag="ACG8Z3_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0824000.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            14408..15106
                     /locus_tag="ACG8Z3_001692"
     CDS             14408..15106
                     /locus_tag="ACG8Z3_001692"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0824001.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            15134..15898
                     /locus_tag="ACG8Z3_001693"
     CDS             15134..15898
                     /locus_tag="ACG8Z3_001693"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0824002.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYKQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEIKKKKFGLF"
     gene            15911..17728
                     /locus_tag="ACG8Z3_001694"
     CDS             15911..17728
                     /locus_tag="ACG8Z3_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFQ0824003.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISTGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASDVSSILILLFINRQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIQVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAKGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNK"
     gene            17742..18965
                     /locus_tag="ACG8Z3_001695"
     CDS             17742..18965
                     /locus_tag="ACG8Z3_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFQ0824004.1"
                     /translation="MNKLEKRQIPFSPPDITDLEIKNVVEVLKSGWITTGPKTKKLEK
                     KITKWLGTSKVVCLNSQTAAAETGLRLLGIGENDEVIVPAYTYSASASVVCHIGANLV
                     LIDCQQDSLEMDYDALEAAITEKTKVIIPVDLGGIPCDYDRIFEIVEKKRNLFQPSNE
                     IQKAYGRIIVMADSAHAFGANWHDQMVGNVADLTTFSFHAVKNFTTAEGGALVWKEHD
                     KLDNEAMYHQCQLLSLHGQSKDALTKTQLGAWEYDIVGPWYKCNMTDVTASIGLAQFE
                     RYPNILKRRKNIIKRYDEAFQQLGIEVLMHYTDDYSSSGHLYITRVPNISSEQRNEII
                     IKMAEQGIACNVHYKPLPMFTAYKNMGFDINNYPNAYAHFVNEITLPLHTKLTDEEVE
                     YVINNYSKIVKDLVG"
     gene            18979..19713
                     /locus_tag="ACG8Z3_001696"
     CDS             18979..19713
                     /locus_tag="ACG8Z3_001696"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0824005.1"
                     /translation="MRKWEDLPKEMQNDEIRYYYDILSKKKFSLVVKRVFDILVSATL
                     LLTLSWLFFILAIVIKIDSPGPVFYRQERITRYGKKFKIYKFRTMVINADKDGTLVTV
                     KNDDRITRVGKVIRKYRLDEISQLINVLVGDMSFVGTRPEVGKYVKSYSPLMLATLLL
                     PAGITSETSILYKDEDKLLHDKNNIDKTYIEKILPSKMYYNLKSISEFSCMNDLKIMC
                     KTIFVIFGKEYHADLEGEREFINQIK"
     gene            19733..20341
                     /locus_tag="ACG8Z3_001697"
     CDS             19733..20341
                     /locus_tag="ACG8Z3_001697"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0824006.1"
                     /translation="MIEEIKSSDKELINSIVDIHMRTFTNFFLTFMGKKFLELMYRSY
                     TEYNDSGILVAIQNDKPIGFLAYSGDMSGLYKYMIKKRIIPFGFYSCVAFIKKPNILM
                     RLFRAFLKPSETKRDENYVELASIGVNPKEKSIGVGSMLIDNLKSRIDFSKYEYIALE
                     TDALNNDVVNNFYKKNEFILERKYSTNEGRIMNEYRFYMEKN"
     gene            20344..21489
                     /locus_tag="ACG8Z3_001698"
     CDS             20344..21489
                     /locus_tag="ACG8Z3_001698"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0824007.1"
                     /translation="MKLLYILNVANKVNNFSKASMMAAKQTGLEFYIAGNWKYKSDEE
                     RKADEVKYGIKIYQVDFYRNPLNLKNIKAYHQIVEIIKNEKIDIIHCNTPIGGVVGRI
                     AGLKCKVKKVIYQAHGFHFYSGAPKINWFIYYPMERWLAHYTDVLITINHEDYTLAQK
                     LRLRKGGKAYYIPGVGIDTNRFMSSTSIRNFRESICKELNIPDTAKIILSVGELNENK
                     NHLSVIHALQKIPLHTDIYYIIAGVGEMRETLQNIANELGLHQRVFLLGYRSDIPKLL
                     AAANIYILPSLREGLNASLMEAMASGLPCIVGNIRGNVDLIEDGKGGFLINPKDEEEI
                     AGAINRLADNKLLCESMGAHNLDKIKDFDLSVVINQINNVYHEIVSK"
     gene            21512..22564
                     /locus_tag="ACG8Z3_001699"
     CDS             21512..22564
                     /locus_tag="ACG8Z3_001699"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0824008.1"
                     /translation="MKKIKIMHLLQSNRFSGAENIVCQIMSLFQNNEKFEMIYVSPDG
                     PIANILNIRGLSYLPLKKFSRKEIDRAVHELNPDIIHAHDFNASVRASRYKKQIILSH
                     LHNNPLWLPKLDIRTIIYSLCSSRFNSVIGVSNSVKEEFRYKQLIKDKFVLLPNTVNT
                     ERIIKMGNEFVEDDIDILYVGRMAEPKNPIGAINVLENVVKKYSKHLRVVMIGEGPLF
                     DEVKMQIQKRNLTDVIELKGFKSNPYKYMKKSKVLLMPSLWEGFGLVSVEAMLLETPV
                     VAYNVGGLKDIIDETNGFLCGSEVELSNSLMKLLNSEELRKKMGENAAISAKKFSNMN
                     RYQGVLEKIYVSAALK"
BASE COUNT         7920 a         3877 c         4671 g         6365 t
ORIGIN      
        1 tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg tttgatttgg
       61 ttattcttct ggtaactcac ttgcttcttg tttatatcct gatacgcgat atacataata
      121 aaagagcaag ctgccagcgt aaccaagcaa tgttgggacg acccagccaa gacctaatgc
      181 agcaaaagga acataatgat gataaaatcc tagtaagctt gctacgacag tttgctgact
      241 cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag aaaagaacat
      301 cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg aaagtaagat
      361 taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt ggatgatatt
      421 atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca gccatgtcat
      481 ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa cgagtcccat
      541 agcagtagtg aagacaccta atgtgaccat tacccctaag aagctagcac cgaagttacc
      601 taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata aagacatctt
      661 attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt ctaacgcaac
      721 gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa aaccaagatc
      781 acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat caaccgtgtt
      841 gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg cagcagattg
      901 gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta ataatgcaag
      961 aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat gtacagtaag
     1021 gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg aatcatattt
     1081 ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc gcggtgtacc
     1141 aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat accattttcc
     1201 gacaggttta gctaagtcat acagaccatc actttttgtc actactacag ctaagatagc
     1261 taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga tccagtttga
     1321 tccagccatt tgacctaagt gtacaggaaa gatcagatta cctgacccga agaacatccc
     1381 gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg tttcttccat
     1441 aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca gaaactagaa
     1501 ataccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa aagccctttt
     1561 ccacttggga aaagggctct agattctaga acggtaccac cttttttcgc ttaaaaaagc
     1621 ttcttcattc acggacgctt ttcgataaag ttatccgcta gcctgataat ggaggcagcc
     1681 acaatgatct acgttttcga cattgactcg gaaatgatgt tcgtctattc gatttattgt
     1741 catttctcac ttacatgact cactgggaat aaatcactga atagcctact gttttcctcg
     1801 acgctttgct gtatatttat ttgttgacgt tataatacac ctgttttttt tgaaatgcaa
     1861 gaatatttca aatattttgt tttctttttt tgaaacaaga aaaataccca gagaatactt
     1921 gctaagttag acggaatact tgtcctctat cttatgcata gcttacatga tcagcttttt
     1981 ttattcaaac gagaccttta gagaatggaa gcggctagtt ttttaacgtt tcttggtact
     2041 tatggttaag caactctttt agaacctttt cttatttgct atatagtttc ttctgagtat
     2101 ggtaaaatag gaaagaatta atcgaaaaaa aggggatgtc tatttttgac ggaagagtct
     2161 agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt aggtgcacac
     2221 gacaagcata taaaactgat tgaggaaaca acggaaacaa ccatccatac acgtggagaa
     2281 atcattcaga tcattggtga gcaatcccag atttccttgg ctgcctcagt cattcggaca
     2341 ttacaggaat tgatcaaacg aggaatccac gtaagtacgc ccgatgttgt gacggcattg
     2401 aaaatggggc aaaaaggtac attagattat tttgttgaga tgtatgagga agagatcgtc
     2461 aaagaccgta atggaaaacc tatacgggta aaaaacagcg gccaaaagaa atatgtcgaa
     2521 tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg aaaaactttt
     2581 cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa aatcattttg
     2641 acgcggcctg ctgtggaagc aggtgaaaat ctaggctttc ttcccgggga tctaaaagaa
     2701 aaagtggatc cttatctgcg gcctgtttat gatgctttat accaaatttt tggattggat
     2761 catacgaacc gattgatgga acgaggcgta atcgaaatcg cacctttagc ttacatgcgg
     2821 gggcgaacat tggatgatgc attcgttatc ttggacgaag cacaaaacac gacagtcgct
     2881 cagatgaaaa tgttcttgac ccgtctcggt tatcaatcaa aaatgattgt caatggggat
     2941 acaagtcaga tagacttgcc tcgagggacg acaagtggtc ttgttcatgc agaacgaaca
     3001 ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt tgttcggcac
     3061 cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca agaataagct
     3121 ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa gaaaaacttg
     3181 gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc ggcgttacat
     3241 ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca gaagaaagta
     3301 tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga aaactggcag
     3361 cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac cagaacaatc
     3421 ggatcaagca actatttgaa ttgatcgata agacgaatga tgccatcaac aaatcttatg
     3481 acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact acagaagagc
     3541 ggatcgccgc gctgaagaaa aattttgaaa atgtgaatgc agaaaatgtt gtgttttatc
     3601 aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact gagattcata
     3661 ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa gttcgtgaaa
     3721 gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg aatgtcacac
     3781 cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg aatgacgtat
     3841 tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta caaccagtga
     3901 tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct tcagctatga
     3961 aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg ctaattgcca
     4021 tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt caattccaaa
     4081 atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca gtcagcatag
     4141 tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc ccgctgtttt
     4201 atcccgctgc ttttgcacca cttgttttaa gctttttctt gaatcgaaga gcagggatca
     4261 tggctggtat tttccaagca gtatccgcac ttttcatttt ttatgattcg attggcacga
     4321 actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca acgattgtca
     4381 gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt atttttcctg
     4441 tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat gcaacgactt
     4501 ggttaatgct cgtctgcggg ttcactggtg cactcttctc attcttgctg acgatcggtc
     4561 tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta aatgaactca
     4621 gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt acttatcatc
     4681 atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc ggacgttctt
     4741 tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat gctagctttt
     4801 tcgttgaaaa cttgccttca ggagcggaaa acccgcacaa tttcttattg ccagaagaca
     4861 gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa gattataata
     4921 tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta atgcgttatt
     4981 tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc cgttatcctg
     5041 gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt tgtgaggctg
     5101 cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc cataatctta
     5161 tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg aaagaaatcc
     5221 gaaaagtgga aaaatcatta atcagcggtt taagtagcac cttccattct cggatcaaat
     5281 atcctaaaat gaaaacagaa gttgagaaga taaaggaagt acaagaagaa tagaaagaag
     5341 cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga agagaaaatg
     5401 aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc agaggaaaca
     5461 gaaatgtctg ttactttcat ggacaatgcg gcaattcaag taatcaatcg tgattaccgt
     5521 ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg cgaagacgaa
     5581 atcccagtga tttttgaaga tggagagaat ccattacctc gtgtactagg agacatcatg
     5641 atctctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata tgatcgggaa
     5701 cttggctttt tagcggtaca tggatttttg catatcaacg ggtatgacca tatgacacca
     5761 gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta tggactcaaa
     5821 agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg ctgtccaagg
     5881 agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt tcggtatcct
     5941 tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat ggctgctttt
     6001 agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa atgtagtgga
     6061 catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata tggcagctgg
     6121 agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat ttgtccctaa
     6181 aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca taaatcaggg
     6241 tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa ccgaatcgtg
     6301 ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa aatccaagga
     6361 gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat ccataaacca
     6421 aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa agaagtggat
     6481 gcagtcttat ttatgatcag tgctgatcaa aaacgtggcc ggggagacga ttttattatt
     6541 gaacgtctaa aaaacgtgca ttctcctgtt tatctgatta tcaataaaat cgataagatc
     6601 catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc ttttgcacaa
     6661 gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga tgtattagtc
     6721 agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga tcatcctgaa
     6781 tacttcatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg agacgaagtg
     6841 cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga taaaatccat
     6901 atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat cggcaaaggc
     6961 ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa tcttttagga
     7021 gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga caaaaaagtc
     7081 tatctgcaag acttcggcta ccgaaaagac gagtactgat attctggact tcttataggt
     7141 taggtataga aataaactgg ataaaaatcc gtacgttgag aaaaacagcg gaagatcgaa
     7201 acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta ggagggagaa
     7261 tatgagccag cttgtagaat cgaaaggctt gattttatct agccgtaatt acaaagaaaa
     7321 agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt atatcaaagg
     7381 aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg aagcagtcta
     7441 tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag acgtccatgc
     7501 gtttcggaac atccaagaag atatcttttt gagcggttat ggtacgtatt tgctaaatct
     7561 agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat ttacgcaaca
     7621 agctctgcag cggatggacc aaggagatga tgcagaaatc atcacgaata tctttgaagt
     7681 gcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg tatgcgggga
     7741 aacaaaagga aaatttgact attcttccaa atatggcggt gtgatatgcg agaaacactg
     7801 gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg ttcgaatgtt
     7861 ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa cgaagcaggc
     7921 aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc taaaaagcaa
     7981 aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag agaaaaatat
     8041 gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa aaaaatcaaa
     8101 actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa tagatcgttc
     8161 actgagagga tctattatac aaaccagaaa actgcaaaat gaatacgtac attgaccaag
     8221 atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac ccaaacgatt
     8281 tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt cggaagtagg
     8341 gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac tttttgtcgt
     8401 tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc aaaacagatg
     8461 agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg gtcatcaaac
     8521 ggctgcatgc tgatgcaagc ttatgataca gaaaaaggag caggtacgat gagcccatat
     8581 acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga gccatcccgc
     8641 cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca tcatcaattt
     8701 caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga aagcttgcgt
     8761 cttttaggaa ttgatccatt agaacacgat attcgctttg ttgaagacaa ctgggagaac
     8821 ccatcgatgg gttgtgccgg tgtgggctgg gaagtatggc ttgatgggat ggagatcaca
     8881 caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac ttctgagatc
     8941 acttatggac tagaacgtct tgcttcttat atccaagaag ttgaaagtgt ttatgatctg
     9001 gaatgggctg atggcgtgaa atatggtgag atcttcaaac aaccagaata tgaacattct
     9061 aaatattctt ttgaagtaag cgaccaagac ctgctactaa gcaattttga tcgttttgaa
     9121 aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga ttatattttg
     9181 aaatgtagcc atacattcaa tttattagat gctcgcggtg cagtatccgt aacagagcgt
     9241 gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca
     9301 gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc agaaaacgtg
     9361 aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg aagaaatgcc
     9421 tgctcatgta gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa aatttttaga
     9481 tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc gcttagcaat
     9541 caaagtaaca aacattccag aacgtcaagc agacagcgaa gaagaagtca aaggaccagc
     9601 caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag gatttgtccg
     9661 cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg tggaatatgt
     9721 ctatgtaacg aaatacactc acggcaaaga agcaaaagaa gtattgacag gtttagcaga
     9781 tgtcattaaa agcctcactt tcccagtaac gatgcactgg gcagaatacg attttgaata
     9841 tattcgccct attcactgga tcgtggcatt gttagatgac gaaataatcc cgtttgagat
     9901 cttggatgtc aaaacagaca gagtttcaag aggtcatcgt ttcttaggcg aagatgtgac
     9961 atttacgcat gcaaaagaat acgaagaaaa attgaatgaa caatttgtta ttgcttcacc
    10021 gcaaaaaaga aaagaaatga tcatcacaca agcaaacgaa ttagcagaaa aaaatcactg
    10081 gatcattgat ctagatgaag atttactaga agaagtcaac aatctagtcg aatatccaac
    10141 tgtcttttct ggcgacttcg aagaaaaata tctatctgtt cctgaagaag tactagtcac
    10201 ttctatgaaa gaacatcaac gttatttcga agtacggaac gatcaaggta tgttgcttcc
    10261 tcattttatt tctgctcgta atggcgatcg ggtaaaattg gaaaatgtgg tacgaggcaa
    10321 tcaaaaagta ttgactgctc gtttagaaga tgcggaattc ttttataatg aagacaagaa
    10381 agcttcaatc gaccaatacg ttgaaaaatt aaaaactgtt actttccatg aaaaaatcgg
    10441 tagcatctat gagaaaatgc aacgggtaca agtcatcgct cgcatcatag gcgaacaagt
    10501 aggactatct gaggaagaat tgatggatct gcaacgcgct gctgaaatct ataaatttga
    10561 tctagtaacc aacatggtag gagaattccc tgaactacaa gggatcatgg gagaaaaata
    10621 tgctctgctt aaaggcgaga aaccagccgt agcaacagcg atccgtgaac attacatgcc
    10681 tatatccagt gaaggagatt tacctgagac tacagtaggt tctgttttag cgattgctga
    10741 taaattagat agtatctttt ctttctttgc agttgggatg atcccaagtg gctcaaacga
    10801 tccatatgct ttgcgccgtc aagcatatgg tgtcgttcga atcatcgaat cgaaaaaatg
    10861 ggcctttcct ttgtctgttc tgcaagaaac gattagtgaa gttatcagta aagatacgga
    10921 tcgttttggc attggcttgt cagcgggaca acaacaggtg atcgacttta tcaaaggccg
    10981 tctgcgccaa ttattgacaa cgaagaatat ccgtcatgat gtcatcgaag ctgttttgaa
    11041 tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca aacaacatct
    11101 tgcagatgaa gacttcaaac cttcaatgga agcattgaca cgtgtcgtga acttagcgaa
    11161 aaaagcgcag cttgaaaaac aatcagaagt agaccctgag ctgttcgaaa acgacgcaga
    11221 gaaagaatta cacaaagcag tagaagcaat gaaagcaagt tttgctgacc aaacgatcca
    11281 cgaaaactac gaccagcttg tcgctcttcg tccactgatc gaaaattatt ttgatcaaac
    11341 gatggtcatg gctgatgaac cagcagtacg taagaaccga ctaacacaac ttagtcaaat
    11401 aactgccatg gctttgtcat tagcaagctt ggatcaaatc atcacaaaat agtcttttgt
    11461 ttgaaaaaca gagagcatgc acgaaacgtg ctgtgtctct gtttttttgt tataaatata
    11521 aaagctgtta ttaattctat tttaataatt tttgttattt tattctttgc tagaattttc
    11581 aaaacgctca tgaaaagtga ggaaataaaa atgaaaaaga ttaataataa tcttgttact
    11641 gttttattta cttttaaatt aggaatttcc ctagtcaaga aaactgtttg tggtatgatt
    11701 atatagggaa aacctgttta taaatatttc tatgaatata ttagtttaat tatgctttta
    11761 aaataaaaag cgctcattct gatgtgaatg aatcacatct ttatttatct atcatcaata
    11821 tttaggagtg aatctatgga aaagccgttc gtcaagttat tggctactgt ggcggtaggc
    11881 acaggcgcta ttgtgatttg cttgtttgga taccatctca ataatcagcg gcaacatcat
    11941 caacggatca attacgcaga atctgccatc accaatcaaa aagacaccgt gacctcttta
    12001 tcaaaagaag tagataagct gtacagtaca aaagaaaaaa ttttcctaaa tcctgaaata
    12061 acagaagaaa cagtttcaaa tttgtcacat aaactatctt ctatcaaact ttcagcagat
    12121 gattatgaca tcaaggaatc agaattacct aaagaagctg ctgcgatcca agaagaaaag
    12181 aaagcagtct taacacagtt agaggatgca gaatcaaaac tgaagatcca aacggctgtc
    12241 aataaattat ttactaaaaa cgtatcaaac tggcagcaag ctgtggatga tgtcatcatc
    12301 aaagaaaagt tggcaagtgc agatgtcgcc catgtacgtg aaaatatgag cttttttaag
    12361 gatagtgctt ggaaaacggt cgttatgcaa tatctaggat tcgctgatac ccaaattgca
    12421 caagtcacgc aactagatca gctattcgat acgatgctga aagacggtca agtaacagct
    12481 actgctactt acgatcaata tttgaccgca ctcagtctca tcgaacagat ccggaatgaa
    12541 aaaataagag ccgcatacgc gtcaaaagca gaaacagtcg ctcagcaaat gggctatagc
    12601 aatacgagct attaataagc gagaattcaa gcaaggctag caaacggagg aaactatggc
    12661 aaaacgaaga agaagatcaa gagggaaaaa gatatttcta attattttag caattcttgt
    12721 cgtattagta ttagcaatcg taggagtagg agcaaaatta tatctagatc tctcacattc
    12781 tattgaaaaa acttacgaat cagtggaacg aacccaagga gaacgaacaa aaccagttga
    12841 tctggacaaa caagaatctt tctctgttct attgatgggg attgatacgg gagatttagg
    12901 tcgggtcgat caaggaagat cagatacgat gatggttgcc acagtcagtc ctgaagacaa
    12961 acagacaacg attgtcagca ttcctcgtga tacgtatgtc gacatcgttg gacatgacac
    13021 aaaagacaag atcaaccacg cttacgcatt cggcggagct gccatggcga tggataccgt
    13081 acaaaaatat gtagacatcc caatcgatca ctatgtttca atcaatatga aaggtctaaa
    13141 agaactcgtt gatgcagttg gtggtatcga agtcaataat gacctcacct tctcacaaga
    13201 tggctatgat tttacgatcg gtaaaatcac attgaacggg gatcaggcat tgtcttattc
    13261 tcgtatgcgt tatgaagatc caaatggtga ctacggcaga caagaacgtc aaagaaagat
    13321 cgtagaaggt gtagcgaaaa aagtgttgag tctgaacggt gtaagcaact atcaatccgt
    13381 tttgaatgct ctagagtcga atatgaaaac agacatgagc tttgatgaaa tgaaaaagat
    13441 tgctttagat tatcgggatg catttggtac gatcaagcaa gatcagctgc aaggagaagg
    13501 cttcatgcaa gacaatatct cataccaaaa agtcagcgat caagaacttg atcgtgtgaa
    13561 aaaagaatta aaagatcaaa tgaatctaga gaataaatag gaaagtggag aaaaaatgga
    13621 agagacaatc agcttgaaag aaatttttga aacattacgc aaacacgtga taacaatcct
    13681 catctcaatg tttgctggct tggccatctc aggcatcgtg acgtttttca tcttgacgcc
    13741 aaaatacagc tcacaagccc aattgatcgt gacactgccg caatcagata ctgcaaatgt
    13801 caatgatgtc aatacgaacc tgcaaatgat caatacgtat aaggatatga tcgtcagtga
    13861 tctagtacta aatgaagtca aagatcgttt agaaacagaa gacaatgtaa aaatgactgc
    13921 tggacaaatc aaagatgcca tctccgtcaa tcagtcacaa aattcacaaa tgttctcgat
    13981 ccaagcaaca agcacaaatg cagtgacggc tcaacaaatc gctaatacaa cgtcacaagt
    14041 attccaagag aacgccaaag acgtgatgaa tgtcgataaa atctcgatca tctcaggtgc
    14101 agttgccaat acgacacctg tttcgccaaa taataaattg aatctagtga ttggcttagt
    14161 gttaggaatg atggtaggcg taggtctagc tttcttattt gaattactcg accggacagt
    14221 aaaagatgaa aaatatgtga cggatacatt gggcttcccg atcttaggga ctgtaccaga
    14281 aatgtcagcg aaagaattga atgctggtat ccgtaaaaaa ggaccagctc cgacacaacc
    14341 aaaagctaca aacgataaaa cagcgccatc acgcagaagc cgctcaagag tctaggagga
    14401 aagaaaaatg gcacgaacac agaaacaaaa agcagcccaa actcatgcag tcagtttggt
    14461 cacactcaca aacccctctt caccaatcgc tgaacaatat cgtacgattc ggacgaatat
    14521 ccagtttgcc tccgcagctg gtcagcaaat taaaacgatt gttgtgactt catcaggacc
    14581 tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt gtttttgcca aatctggtca
    14641 acgggtgctg ctagtcgatg cagacttacg taagcccgtc atctataaaa cgttcaagtt
    14701 gaacaacgca agcggattaa gtaccgttct tagttcatca ggaagtgtag cagatgagat
    14761 ccaacgaaca ccagtcgaga atttgtcgat tctgccaagt ggacctaaac caccaaatcc
    14821 atcagaacta ctaagttcgc caagaatgga tcagatttta gcagaagcaa gacaactatt
    14881 tgatgtagtg atcttcgaca tgccgccagt agttgccgta acagatgcac agatcatgtc
    14941 ttccaaaacg gatggcacct tattggtcgt acgtgaaaat atgtcacgga aagaatcatt
    15001 gactaaagca aaagaattgc tggatatggt acaagcgcga gtccttggtg ttgtatacaa
    15061 tggggcagaa cacagcaaag atgctggcta ttactacggg aattaagaaa taaaaagaga
    15121 ggagacaaga gaaatgatcg atctacattg ccatatcctg ccaggaatcg atgacggagc
    15181 agaaaacata gatgccagta tcgccatggc tgaaaaagcg attcaacaag ggatcacaca
    15241 tattttatgc acaccccatc ataataatgg aaaatacagc aatgaaaaat cacaagtcat
    15301 ttctctagtc gcttcgttac aagcagaatt agagaaaaga caattgcctt tgacgttatt
    15361 ggaaggacaa gaagtacgta ttaccggaac actgatcgaa gacatcgacc gagatgaaat
    15421 tttatttaca gatttagatg atacatatct tctaatcgaa tttccgacac tggaagtgcc
    15481 gttatatgca gaacagctct tttttgagtt gttacaacta ggaaagacac cagtgatcgt
    15541 ccatccagaa cgcaacgcgc attttcgtaa agacccgaat catatgctgc cattcctaga
    15601 tatgggatgc ttggcccaac tcaccgcacc aagttatgtc ggtgtatttg gaaaagacat
    15661 ccagaaaaca gccaaaatga tggtcaaaca taatcttgtg cagatggctg cttctgatgc
    15721 ccatggggtc tctaagcgca ccttttactt aaaagagtgc tataagcaga ttgccaagga
    15781 ctttggtaag gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga
    15841 tgaaatcagt tatccagtct atgaagagat aaaaaagaag aagtttggtt tattttaggg
    15901 ggagtgtaag gtgtttgtat taacaagaag aagaaaaatt gccacattgg ttgttgtgga
    15961 cagtttgtta ttgatcgctg caaatattgc agcagctaaa ttcatgaagc cgtttgtagc
    16021 tataccaatg gaccttattt taatctctac cggtttgtcg attggttttt atttatttta
    16081 tggtagttta ttcaaagttt tcactcggat caatcgctat acaaatttaa gagagatcat
    16141 tgcaattttt ggttcactat ctgcaagtga cgtctcaagt atcctcatac tgctgttcat
    16201 taatcggcaa tacagtttac ggttagttat ctttgcttat ctgctttcgc tcttattgat
    16261 catcggtagc cgattgatct ggcgtatcta catcgaaaca aaaaatatga gatatgtatc
    16321 agcggattct gctaaaaata cattgatcgt tggtgctggc gaaggcgggc gtattttata
    16381 caacagcttt ctgggatcca aaacggctca agatatccaa gttgtcggat ttgtagatga
    16441 tgatccgaat aaaagaaata cgtatctttc aggaaaaaaa gtattaggtg cattaaaaga
    16501 tattccggaa ttgattgaaa aatatgacat tcaaatggta acgatcgcta tcccttcctt
    16561 atcaagaaaa aaattacgaa gaatcttcga gttagtcgaa tccgctcacg tgaaagtcaa
    16621 cacgatgcct tctatagaag aacttgcttc aggaaaaatt agcgtatcaa aattaaaaac
    16681 aattgacgta gtggatttac tgggaagaga tgaggtaaag ctggatattg aatccatcaa
    16741 agatcagatc acagacaaag tcattcttgt caccggcgca ggaggatcga tcggttcaga
    16801 aatctgtcgg caaatcattc aattcaatcc tgcaaagcta ttgcttttag gacatgggga
    16861 gaactcgatc tacttgattg accgtgagtt acgcacgcat catcaaaatt gtcctacaga
    16921 aatcgtaccg atcatcgcag atatccaaga tcgcgagaaa atcaatgaga tcatggaaca
    16981 atatcatcca gatatcgtct accatgcagc tgctcataaa catgtaccac tgatggaata
    17041 taatccaaaa gaagcagtca aaaacaatat tttcggtaca aaaaatgtag cagaagcagc
    17101 taaagcagca aaagtgaaaa acttcgtcat ggtatcgaca gataaagcta acaatcctcc
    17161 aaatgtcatg ggttcaacaa aacgaatcgc tgaaatgatc gtgactggat tgaatgaaga
    17221 aggctgtacg aaattctcag ctgtcagatt tgggaatgtc cttggttctc gcggtagtgt
    17281 gattccagta ttcagagaac aaattgctaa aggtggtcca atcactgtaa cagactttcg
    17341 tatgacccga tacttcatga cgattcctga agctagtcgt ttagttattc aatctggcgc
    17401 attagcaaaa ggtggcgaaa tttttattct agatatgagt gaaccagtga agattgtaga
    17461 cctagccaaa aacatgatcc gattgagcgg gtatagcgaa gacgaaatcg aaatcattga
    17521 aacaggtatc cgtccagggg aaaaactata tgaagaacta ctgttggata aggaaagaaa
    17581 tgatgaagca gtttatgaga aaatctttgt aggaaacatc aaaggttact caatacaaaa
    17641 agtgatggat tttgtcaaga gcctgccgca agacgatgaa caattggcta aagatatcgt
    17701 cacttttgca aatgcttcga ataaataggg gggaaatata aatgaataaa ttagaaaaac
    17761 gtcagatacc attttcaccg ccagatatta ctgatttaga aataaaaaac gttgtagaag
    17821 tattaaaatc tggatggata actacgggac caaagacaaa aaaattagaa aaaaaaatta
    17881 caaagtggtt aggaacttct aaagtagttt gtttaaattc acaaactgct gctgcagaaa
    17941 cagggttacg tttgctggga ataggagaaa atgatgaggt tatcgtccca gcttatactt
    18001 attctgccag tgcatcggtt gtatgccaca taggcgcgaa cctagtatta atagactgtc
    18061 aacaagattc tttggagatg gattacgatg cattagaagc agcaattacg gagaagacaa
    18121 aagtgattat tcctgttgat cttggtggta taccttgtga ctacgaccgt atttttgaga
    18181 ttgtggagaa aaaaagaaat ttatttcaac ctagtaatga gattcagaaa gcttatggtc
    18241 gtataattgt tatggcagat tctgctcatg catttggagc taattggcat gatcagatgg
    18301 ttggtaatgt tgctgacttg acaacatttt catttcatgc tgttaagaat tttacaactg
    18361 cagaaggtgg agctttagta tggaaagagc acgacaaact agataatgag gcaatgtatc
    18421 atcagtgcca gctgctttct ttgcatggac agtcaaaaga tgcacttact aaaacacagt
    18481 tgggtgcatg ggaatatgat attgtgggtc catggtataa atgtaatatg actgatgtta
    18541 ctgcatcaat tgggttggca caatttgaac gctatcctaa tattctgaaa agaagaaaga
    18601 atataattaa gaggtatgat gaagcctttc aacaactagg aattgaagtt cttatgcatt
    18661 atacggatga ttatagttct tcaggccatt tatatattac tagagtacct aacattagtt
    18721 ctgagcaaag aaatgaaata attataaaaa tggcagaaca agggattgct tgcaacgttc
    18781 actataaacc acttccaatg tttacagcat ataaaaatat ggggtttgat attaataact
    18841 atcctaatgc ctatgctcat tttgtaaatg agattacatt accattgcac acgaaactta
    18901 cagatgaaga agtagaatac gtaattaata actatagtaa gattgtaaag gatttggttg
    18961 gatgatagga gaggcaaaat gagaaagtgg gaagatttgc caaaagaaat gcagaatgat
    19021 gaaatacggt attattatga tatcctcagt aaaaaaaaat tcagcttagt ggtaaaaaga
    19081 gtatttgata ttttagtgtc tgcaactttg ttactgacac tttcatggtt attttttatt
    19141 cttgcaatag tgataaaaat tgattctcct ggacctgtat tttatagaca agaacgtatt
    19201 actcgttatg gaaaaaaatt taaaatttat aagtttcgta ctatggtaat aaatgcagat
    19261 aaagatggca ctcttgttac tgtgaaaaat gatgatcgta ttacaagagt aggaaaggta
    19321 atccgtaaat atagattaga tgaaattagt cagctaatta acgttttggt cggagatatg
    19381 tcttttgtag gaaccagacc agaagttgga aaatatgtaa agtcgtattc accattaatg
    19441 ttagccacat tattattacc agccgggatt acatcagaaa ctagtatttt atacaaagat
    19501 gaagataaat tgttgcatga taaaaataac atcgacaaaa cttatataga aaaaattctt
    19561 cccagtaaaa tgtattacaa tttgaaaagt atcagtgaat ttagttgtat gaatgatttg
    19621 aaaataatgt gtaaaactat ttttgttata tttggtaaag aatatcacgc cgacttagaa
    19681 ggtgaacgag agtttattaa tcagataaaa tgaatggagg ataagagttt tggtgattga
    19741 agagataaaa agttcggata aagagttaat taattcgatt gtcgatattc atatgagaac
    19801 atttacaaat ttttttttga ctttcatggg aaaaaagttt ttggaattga tgtatcgttc
    19861 ttacacagaa tataatgatt ctggtatatt ggttgctatt caaaatgata agccaatagg
    19921 atttcttgca tattctggag atatgagtgg tttatataag tatatgataa agaaaagaat
    19981 aataccgttt ggattttata gttgtgtagc ctttataaag aaacccaata tccttatgag
    20041 gctttttaga gcttttctaa aaccaagcga aacaaaacgt gatgaaaatt atgtggaatt
    20101 agcatccatt ggagttaatc cgaaagagaa atcgatagga gtaggttcca tgcttataga
    20161 taacttaaag agtagaattg atttttcaaa atatgaatat attgcactag aaacagatgc
    20221 attaaataat gatgttgtga ataattttta taaaaagaat gagttcattt tagaacgtaa
    20281 atattctact aatgaaggaa gaattatgaa tgaatatcgg ttttatatgg agaagaatta
    20341 attatgaaat tattgtatat tttaaatgta gctaataaag taaataactt ttctaaagct
    20401 agtatgatgg cagctaaaca aacgggttta gaattttata tagctggtaa ctggaaatac
    20461 aaatccgatg aggaaagaaa agctgatgaa gtgaaatatg gaataaaaat ataccaagtt
    20521 gatttttata gaaacccact aaatttaaaa aatataaaag cttatcatca gattgtagag
    20581 attataaaaa atgaaaaaat agatattatc cattgtaata cacctattgg aggtgttgtc
    20641 ggacggattg ctggtcttaa atgtaaagtc aaaaaagtaa tttaccaggc acatggattt
    20701 catttttatt caggtgctcc taaaataaat tggtttatat attatcctat ggaacgttgg
    20761 ctagctcatt atacagatgt tttgattaca ataaatcatg aggattatac tttagctcaa
    20821 aaacttcgtc ttaggaaagg tggaaaagct tattatattc caggtgtggg tatagataca
    20881 aataggttta tgtcttccac atcaataagg aattttagag aaagtatatg taaagaactt
    20941 aatatacctg atacagcaaa aatcatttta tctgtaggag agctaaatga gaataaaaac
    21001 catttatcag taatacatgc attacaaaaa attccgctgc atacagatat ctattatatt
    21061 atcgctgggg taggagaaat gagggagaca ctacaaaata ttgctaatga attaggatta
    21121 catcagcgag tttttttact aggatataga agtgatatac ccaaattatt agctgctgca
    21181 aacatatata ttctaccctc attaagggaa ggcctcaacg cctctttaat ggaagcgatg
    21241 gctagtggtt taccatgtat tgtcggtaat ataagaggaa atgtagatct tatagaagat
    21301 ggtaagggtg gatttctaat caatcctaaa gacgaagaag aaattgctgg tgcgataaat
    21361 agattggctg ataacaaatt attgtgtgaa tctatggggg cacataattt agacaagata
    21421 aaagattttg atttatcagt tgtgataaac caaataaata atgtttacca tgaaattgta
    21481 agtaaataat tgaattgaaa gtgatattaa tatgaaaaaa ataaaaatta tgcatttgtt
    21541 gcaaagtaat agattttctg gcgctgaaaa tattgtttgt caaataatga gtttatttca
    21601 aaataacgaa aaatttgaga tgatttacgt tagcccagat ggtcctatag caaatatttt
    21661 aaatattaga ggtttaagtt acttaccatt aaaaaaattt tcgagaaaag aaattgacag
    21721 agccgtacat gaattaaacc cagatataat tcatgctcat gattttaatg ctagtgtaag
    21781 agcaagtaga tataagaagc aaatcatcct ttcccatttg cacaataatc ccttgtggtt
    21841 acctaaacta gacataagaa ctattatata ttctttatgt tcgtctaggt ttaattcagt
    21901 aattggtgtt tcgaattccg ttaaggaaga atttcgttac aaacaattaa taaaagataa
    21961 atttgtgttg ttacctaaca ctgtaaatac tgaaagaatt ataaaaatgg ggaatgaatt
    22021 tgttgaagat gatattgata ttttatatgt aggacgcatg gcagaaccta aaaatccaat
    22081 aggtgcaatt aatgttttag aaaatgtagt aaaaaaatat tcaaaacatt tacgtgtcgt
    22141 gatgattggt gaaggaccat tatttgatga agttaagatg caaatacaaa aaagaaactt
    22201 aacagatgtt attgagttaa agggattcaa aagtaatccc tataagtaca tgaagaaatc
    22261 taaagttcta ttaatgccat ctctttggga agggtttggc ctagtatcag tagaggcaat
    22321 gttgttagag acgcctgttg tagcttataa cgtaggagga ttgaaggata ttatcgatga
    22381 gacaaatgga tttctgtgtg gtagtgaagt tgaattatct aattcactaa tgaagctatt
    22441 aaattcagaa gaacttagaa aaaaaatggg agaaaatgct gctattagtg caaagaaatt
    22501 ttctaatatg aatcgttatc aaggagtatt agaaaaaatt tatgtatctg cagctttaaa
    22561 ataaaatatg aattataaaa atagagatca attagtttta cgaaaaataa tacccattag
    22621 tattttgttt caatcaggaa tgatttttta ttcactgatg ccctcaatag caattgcagt
    22681 tgtttctttt tttatgtttt tgtattatat gtggtgctta aaaaagtatt tttgtttagc
    22741 agatagctta ttgttaacat caattctttt cattccaaca tcaactatat ctatattagg
    22801 aactagttat tctagcttac cattgacgtg gca
//
LOCUS       DAZKSU010000043        22186 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000043 DAZKSU010000000
VERSION     DAZKSU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.043"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..2091
                     /gene="tdc"
                     /locus_tag="ACG8Z3_001700"
     CDS             256..2091
                     /gene="tdc"
                     /locus_tag="ACG8Z3_001700"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0824009.1"
                     /translation="MKDMDIKVVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2396..3874
                     /locus_tag="ACG8Z3_001701"
     CDS             2396..3874
                     /locus_tag="ACG8Z3_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0824010.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3840..5171
                     /locus_tag="ACG8Z3_001702"
     CDS             3840..5171
                     /locus_tag="ACG8Z3_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0824011.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5285..7837
                     /locus_tag="ACG8Z3_001703"
     CDS             5285..7837
                     /locus_tag="ACG8Z3_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0824012.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7844..9283)
                     /locus_tag="ACG8Z3_001704"
     CDS             complement(7844..9283)
                     /locus_tag="ACG8Z3_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824013.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9258..9440
                     /locus_tag="ACG8Z3_001705"
     CDS             9258..9440
                     /locus_tag="ACG8Z3_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824014.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9629..9991
                     /locus_tag="ACG8Z3_001706"
     CDS             9629..9991
                     /locus_tag="ACG8Z3_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0824015.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10178..11035)
                     /locus_tag="ACG8Z3_001707"
     CDS             complement(10178..11035)
                     /locus_tag="ACG8Z3_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0824016.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11155..12066
                     /locus_tag="ACG8Z3_001708"
     CDS             11155..12066
                     /locus_tag="ACG8Z3_001708"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0824017.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12073..13719
                     /gene="mdcA"
                     /locus_tag="ACG8Z3_001709"
     CDS             12073..13719
                     /gene="mdcA"
                     /locus_tag="ACG8Z3_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0824018.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13821..14120
                     /locus_tag="ACG8Z3_001710"
     CDS             13821..14120
                     /locus_tag="ACG8Z3_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0824019.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14110..15786
                     /gene="mdcD"
                     /locus_tag="ACG8Z3_001711"
     CDS             14110..15786
                     /gene="mdcD"
                     /locus_tag="ACG8Z3_001711"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0824020.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15790..16383
                     /locus_tag="ACG8Z3_001712"
     CDS             15790..16383
                     /locus_tag="ACG8Z3_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0824021.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16407..17483
                     /locus_tag="ACG8Z3_001713"
     CDS             16407..17483
                     /locus_tag="ACG8Z3_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0824022.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17508..18377
                     /gene="mdcB"
                     /locus_tag="ACG8Z3_001714"
     CDS             17508..18377
                     /gene="mdcB"
                     /locus_tag="ACG8Z3_001714"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0824023.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18488..19252
                     /locus_tag="ACG8Z3_001715"
     CDS             18488..19252
                     /locus_tag="ACG8Z3_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0824024.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19741..21834
                     /locus_tag="ACG8Z3_001716"
     CDS             19741..21834
                     /locus_tag="ACG8Z3_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0824025.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21859..22161
                     /locus_tag="ACG8Z3_001717"
     CDS             21859..22161
                     /locus_tag="ACG8Z3_001717"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0824026.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7181 a         3788 c         4727 g         6490 t
ORIGIN      
        1 ggacagtcat tttatttgtt tgttcgcttt ttttaggtta tctattttag aagactgatt
       61 tattgaaaaa ttgttcgctt ttttgcgttg aattatcaga aaaatccgac tttatttatt
      121 tttgtttgta aaaatctacg tggatttgat gttataaaaa aaaaattctc atttttggcg
      181 ctatagtgaa gaggtgttac agataaacac atgtatctgc aagacattga ccaacaaaat
      241 atggagggaa gaaaaatgaa agatatggat atcaaggtcg tctttatagg cgacaaagct
      301 gaaaatggac cagtttataa aatgttgttg aacaagatgg tagatgaaca tcttggatgg
      361 agagaaaatt atattccttc agatatgcct gcaattagcg aaggagataa gctcactcca
      421 gattatctag ccacccgcga ccatatgata gaggtcttgg atgaagtcag tcagcgatta
      481 cgcgcaggat cgatcccatg gcattcagca ggccgttact ggggacaaat gaatgctgaa
      541 acattgatgc ctgctttatt agcttacaac tatgcgatgc tgtggaaccc aaataatgtt
      601 gcattggaat cttctatggc aacttcgcaa atggaagcag aagtaggaca ggattttgct
      661 tcattattca atatgacaga cggatgggga cacattgcag cagatggttc tattgctaat
      721 cttgaaggat tatggtacgc ccgttgcatc aaatcgattc ctttagctgt caaggaagtg
      781 ctgcctgaaa aagtgaaaaa aatgtctgag tgggaacttt tgaatttatc tgttgaagaa
      841 attttggaaa tgaccgaaag ctttacggac gaagaaatgg atgaggtcaa agcagcttct
      901 tcccgtagcg gaaaaaatat ccaaagatta gggaaatggc tggtccctca aacaaaacac
      961 tattcttgga tgaaggcgtt agatatttgc ggagtcggct tagatcagat ggttgctatc
     1021 ccagttcaag aggattatcg aatggatatt aatgctttgg aaaaaacaat tcgtgaatta
     1081 gctggacaaa aaatcccaat tctaggtgtg gttgctgtcg taggaacgac agaagaaggt
     1141 caagtagaca gtgtagataa aatcgtccaa ttaagagaaa ggttaaaaga tgaagggatc
     1201 tatttctatt tacatgttga tgccgcatat ggcggctatg cgcgttcact gtttctaaac
     1261 gaagcaggag aatttgtgcc gtatgcttcc ttagctgaat tctttgaaga acatcatgtt
     1321 ttccaccact gcgtaacgat tgacaaagag gtatatgaag ggttcagagc tatttcagaa
     1381 gcggattctg tcacgataga tcctcataag atgggctatg tcccttatgc tgctggagga
     1441 atcgtaatca aacataaaaa tatgagaaat atcatttctt atttcgcgcc atatgtgttt
     1501 gaaaaatcag taaaggctcc agatatgtta ggagcttata ttttggaagg atcaaaagct
     1561 ggagcaaccg ccgccgcagt ctggacagca catcgtgtat tgccattgaa tgtgaccgga
     1621 tatggtcaat tgattggggc ttcaatcgag gcagctcaaa gattcagaga atttctcgat
     1681 catctgactt ttactgttaa aggaaaaaca atcgaagtct atccattgaa tcatcctgat
     1741 tttaatatgg tcaattgggt attcaaagaa caaggctgta cagatttgaa cgctatcaat
     1801 gaattaaatg aaaaaatgtt cgatcggtct tcttatatgg atggcgatgt ttatggtgaa
     1861 cggtttatta cttctcatac tacatttaca caagaagatt acggtgattc tccgattcgt
     1921 tttgttgaaa gaatgggtct aacgaaagaa gaatggaaaa aagaacagaa aatcactctg
     1981 ctacgtgcgg ctatcatgac tccttatttg aatgatgatc gaattttcaa tttttataca
     2041 aaaaaaatcg ccaaagcaat ggaaaagaaa ctaaacgaaa tcatccaata gaactgattt
     2101 ttgtgtgaaa gaataacaag agacatatgg agaacagaca attatttgct tcatcaaaac
     2161 aattgtattt ccccctgttt ttcatatatt cctcgccaag atattctttt ttataaaacg
     2221 ttctataagt tggatcgttc gtttcatgta tcaaacccag ttcactaaga tcgttcaatg
     2281 gatgtttcgg tgatactggg ttttgtactg aatcgatgaa accttgtcat ttctgttctt
     2341 gaacaaaaat aaaagaaaac aaaaacatat gtcgctattg aaagaaggaa taaagatgaa
     2401 agatactggt tcatcaaagc aaatcacgac catgaccttt atcggaatga cctgcgccct
     2461 tgtagcaagt gtacgaaata ttccagatgt agcagcaacg agctggacga tgatttttta
     2521 catgctggtt gcagtattat tgtacgcttt tccaatctca ttgatctctg gtgaatttgc
     2581 tggaatgttt ccccaaaaag gtggtccaga actttgggta tcgaatgctc taggaaaaaa
     2641 atgggggttc gttgtctcat ggctgttatg ggtccaaatg tttcctggga tggtgatggt
     2701 cgcttcagca ctcgctccgt tgtttggtaa tattatcgat aatgtgccgc ttggtttgaa
     2761 cagcaaattt acattagttg tcattttagt cgtttattgg atcatcactt ttttgaattt
     2821 gaaatttgat atggcaaaaa tcggcggaaa agtcggtgtt tggttaggat tgtacgtgcc
     2881 tttgacgatg atgctcttat tagggtttgc tgcgtggatc aaagttggga tcgtaccaac
     2941 cggaacatta ggcagttttt catgggacaa attaatacca gatactacga ctgcatcctc
     3001 atttgtttat tttgcgccga tcatgtttat ttttactggt atcgaaatgt cctctgtgta
     3061 tatcactcga ttggaaaatc cagttaaaac gtatattcgc ggagtatttg ccgcgttaat
     3121 ttttatattt ttcgtcaata ttttgaatgc tttagttgta gcaaatgtcg taccaaaagg
     3181 acaaatggaa ttgaataata tcgcgcaatc tatttcgatt tattgccaaa ttcttggttt
     3241 gccacatctt attgtgaatc tgtttagttt acttgtcttc attggtgtag cagtccaatt
     3301 atctgcttgg gcctcaggac cagcaaagac agtgacagaa agtgctagaa aaggtgcgta
     3361 tccaccaaaa ttcaattttt ggaagacgaa tcaatttgac gtatccaaat ccgttatttt
     3421 gactcagtct gtcattatct caatctttgc acttttttat ttgctgattc ctggcgtcaa
     3481 tcaagcattt ttgatgctcg taaattcaac tactgttatc tactgtatcg tctatgtcat
     3541 catggggatc gcagttttga gattgcgtta tacacatgca aagttgaatc gaccatttag
     3601 aattggaaaa aaagagaaaa agagcaatct tggagtttgg actgtcgtta ttgttttatt
     3661 tgcggcaatc ggattttctg tcggactgac gatgaaagct ggaacatgga tcaatctaat
     3721 tgcagtcaca gccatcagtg tcattttgtt tgtagtacca ctctgtattg aaaaaataaa
     3781 gaaaccttct tgggaacagg aagtcgccat gctgcttgaa aaagaaaaag gtgaacttga
     3841 tggaaaaacc ggcaatgaaa cggactctaa ctaatcgaaa tatccagtta atcgcattag
     3901 gtggcgcaat tggaacagga ttatttttag gttcagcaaa ggcaattgca tcagcagggc
     3961 cagctgttat tctcgtttat ttgatatgcg gtttggctat ttatggtttg atgcgggcca
     4021 tgggtgattt atttttagca aatagtacat ttaactctat cagtgatttc gttgactatt
     4081 atttaggcaa aaagtgggca ttttttgtca attggactta ttggctttgc tggcttggag
     4141 ctggacttgc ggaattaaca gcaataggat tgtatatcca tttctggttt gtacatgtac
     4201 cagctttgat aagcggaggt agcgcactaa tgatcttaat ggcagtcaac cttgttgcag
     4261 tcaagtggtt tggtgaattg gaatcttttt tctcaatcat caagatcttg gggattttcc
     4321 tttttatctt attgggtata ggattgagtg tctcagattt tcatacccag tctatcggaa
     4381 tcaaagaaaa ttttggacag atcgatgaat tactaccgtt tggctttgct ggaatggtag
     4441 tctcatttcc tatggtttta ttcagttttg ctggaattga aatgatcggc ttaaccgttg
     4501 gcgagacaag tgatccgcaa aagaatttga aaaaagcaat caactgtctg ccttggcgta
     4561 ttctcttttt ctatatagga acgattttgt cgattttatt tgttgttcca tggcattgtt
     4621 tagatttaaa agaaagtcct ttagtgacta tgctgcaagt gatccattgc caaggcggag
     4681 cagcaattgt caatctaatg attttggttg cttctttgtc ctcagcaaat agtgcagtct
     4741 acagtactag caggatttta tatcaaacag caaaagaaaa aagtttgccc cattggtttt
     4801 gtgcgctttc taagaaaggc gtacctttac acggtatttt aattacagga atgttatttt
     4861 ttgccggtct ttgtctacat ttttttgtag ctgatagtcg attgtttttt cagcttttgg
     4921 caggcatgac aacgagttgt tttctatttg tgtggtcttc gatcttgtgt gcacatgttc
     4981 aatttattaa agaaaagaaa cggaacacta cacaaagata taaagatctt gctttgttgg
     5041 tgttttttac ttcaattgct ttcagcttgt tattcgaaag cacgacacgc tttattcctt
     5101 ttttattggt tatctggttt gtattcttaa attttgtata taaaagaata agcaatagaa
     5161 atatcagtta ggcccagata cattttgcaa aacgaattat ttcagacccc cctgaaataa
     5221 tcgatacgcc tgaaaaaact tccctgcgta taataacagt atcaattaaa caggagggga
     5281 taggatgaac tggtacagat tgacggtaga agatgtattg aaaaactatc aaacatcaac
     5341 agaaggcttg tcgcaagaag aacgacagaa acgcctgact gatcaaggat ataatgaaat
     5401 aaaagcaagg cagcagacaa aacgatggaa aaaaatagca aagcatttta ctgatctgct
     5461 gatgatcgtg ttgatcattg cctctttttt gaaatttgct tctagtgaat atattgaagg
     5521 aagtattatt ttattcgtag tgatcgtcaa tggactcgtt agttactggc aagaaagaaa
     5581 agcggaagaa tcattgaatg gtttgaagca gctgatggga caagaagcga tagtactatc
     5641 aaacgggatg caagaaaaaa tccctgcgaa agaactggta aaaggagatg tcattgtctt
     5701 acgtgccgga gatgtcgttc cagcagatat ccgattaatc gaagcacatg atttattagt
     5761 ggaagaatcg attttgacgg gagaatcaga agcatctgaa aaaagtcatg ccgttttgac
     5821 agaagaagaa tcgatcggcg accagaaaaa tatgggtttt tcaggaacgc ttgtacaatc
     5881 tggttctgca gtcggtgtcg ttgtagaaac aggaaacacg acagaaatcg gtaaaatcaa
     5941 tcaagcattg caatcggtcg aacaacaaac gacgcctctg attaaaaaaa tcaaccgatt
     6001 aaacaaacaa atttttcaag gcattctttg tttgagccta ttcttagtta tttttacgac
     6061 atttcgatat ggtatggatt ggcatatcct tctttcggct acgattgctt tggtggtgtc
     6121 aatggtaccc gaaggattgc cagctgtttt gaccatgatt ctatccgttg gagtacatga
     6181 aatggcaaaa gaaaaggcga tcatcaaagg acttccttcg gtagaaacat taggttctat
     6241 gacagtgatc tgttcagata agacaggaac attgacaaaa aatgaaatga cagttgtgga
     6301 tgtagctgca aaagaagaag cctgcgtgtt gtccattatg aaaaactgtc aggaattaaa
     6361 aacaaaagaa gaacagaaaa cagaaaattt aagtggcaat ccgacagaag ttgcgctgat
     6421 tcgacacaca gaaacagcca atctaccttt gaaaaaaata gaagcaaaga ttccgttcag
     6481 ttctgaatac aaatatatgg caacgatgca tgcggaggaa aaaggagcgg tgatttacgt
     6541 caaaggtgca ccagaagtat tatttgctaa atcaactctt tcttctgcag aacaagaagc
     6601 atggagtcag acagctgcag aatttgcgag aaaaggtcag cgtgttttag gatttgctta
     6661 taaaaaagta gattcgaaac aggaactcac gcatgagact ttaacccaat taacttttgc
     6721 agggatcgcc ggattgatcg atccgccaaa agaaagtgca gtcaaagctg taaaggaatg
     6781 ccaacaagca gggatctcag tgaaaatgat tacaggggat cacaaagaca cagcaaaagc
     6841 gattgcggaa caaattggct taaaacatac tgccaaagtt ttagaaggga tcgatttgga
     6901 tcttatgtct gatgaagagt tgattcagca agtgccaata gtcgatgtct ttgctcgaac
     6961 gacaccagaa cataaactgc gaattgttaa agctttgcag aaaaatggag aaatcgtcgg
     7021 aatgaccgga gacggcgtaa atgatgcacc tgctttgaaa agatccgatg taggaattgc
     7081 tatgggaatc aaaggtagtg aagtaagcaa acaggcagct gatatggttt taggagatga
     7141 taatttccat acgattgcaa aagccgtaaa agagggccgg cggattttgg ataatttgca
     7201 aaaaaccatc aatttcttct tgccaactgc attagctcaa ggattgatct tgatttgggc
     7261 acttatgctg aatcgcccgc tacctttgtc cccagtacag attttatggg tcaatatggt
     7321 aacgacaatc actttatctt atgcactagg gttcgagtcg ccagatccag aaatcatgaa
     7381 gcgcccacca agagatccaa agcaaggaat cctatctggt tatcatttgt ttcggatcat
     7441 ctatgtctcc ctactgatca tggttcctgc ctatttattg gctatgcagt ttgaagggca
     7501 aagcttacag caaaccattc ttctgcaaaa tatcgtctta gctcaagctg tttacatgat
     7561 caattgcaga gagttgtcca agccgtcatt gaaccaaggt cttttgcaaa acaaagcgct
     7621 ttttctttca ttaggtatcc ttgctttatt gcaagggatg gttatttttc taccagtcgg
     7681 ccagcagctg atcggtacag taagtttaac gatgacacaa cagttattga ttggagcaaa
     7741 tgtgattctt ctattcttag ttgtagaaat agaaaaaggg atcatgaaca aactgtttcg
     7801 caaaaaagaa aaaacagcta gagaatacag tcgctagccg ttactataaa aaagttttga
     7861 aagtgagttc agtcaaacga ttgagctcat ttttcgttgg aatagaagaa atgtatacaa
     7921 tagggatatc tagagaatct agagggaaat tggaaatcaa gatatctgtt ttttcaaaag
     7981 aaatagtgga aagatcggtt agttcgatag gtaagaagca gacacgtttt cctaagtaat
     8041 cttttaattc ctgctgcaag aacgccttcc atgaaggttc tccttggaaa ataaaataga
     8101 cattgaacaa attcggtttg atagaaaaaa tcgtttgttg gatcagtaga gacaagagtt
     8161 ggagaaagta atttggttca ttgatttcta tttctttttt tagatcgttc agtaaattct
     8221 ctttctgttg atacaagtgt gcatattgct gatcatatgt ttctaaaaaa cgatggtatt
     8281 gacggtccaa tgcttcaatc aatatcggcg attcatgata ttttaatatg aggagagcca
     8341 agttatcagt cagagatgtt ttttttagtt gatcaagaga cagctctgaa gctagttcct
     8401 ctacaaacgt ttcgaggacc tctaaaaatg gagaatgatg caggcagcga gcggtttctt
     8461 gtccatagtt attgttgtaa ttccaacttt ccagaaagca gaaagaagca aaagaaagct
     8521 cagtgtcttt aagataaata ccttccgtta aatacaattt tttcaatgca gagtcataca
     8581 gggaggagag tgtgctgttg gtatggatag aagaatctaa tcggcaattc gcttttatac
     8641 ggatcgtatt gataaagaac caacatgttc cgaaaatctc ttctgtttca accgctagtt
     8701 cagtttgact gaggtttctt aaaaattgaa aataatggga ttcatggatc gaataatcct
     8761 cgaaaaaata gttgttatga gtgaaaggaa tcagcaatcg atgataaaaa gtacgaatcg
     8821 cacgctcttt tccaagaata caagctgtat atttcgtcaa atcgatctgc aagtcgtaac
     8881 ttgctaaacg ctgattgatt ttagtcacat gtcttttcaa tgtcccataa gaaagcccat
     8941 ggtcggctag aaaatcaggg acagcaactt tttttgtaaa aagcagattt ttcagcagtt
     9001 cgacactgac tgattgtttg aaaaaaatct cccaaaggtc atttgttaat aaattttgct
     9061 ggttttgcaa actgattcct gcttgatcgc tgatgatgtc ccagccttcg ggcagctgag
     9121 agcgaataga ttgaaggtca gaaaaaatcg ttcgttcggt tgtccctatg atttcagcta
     9181 attcatttac ggttatcttt gtatgattca gcaattgttc tactaaagaa acatgacgtc
     9241 gaatatcttt ttcggtaatg atttttttca ttagtgaata catcaatcag actcccttct
     9301 attctaagaa tacggagaaa ataaacggtg gtcaagtttc aactgatttt tcaatcattg
     9361 tttatttttc tggagcattt actgatgata agaatttcat catgaacttg aaaaatgata
     9421 ccactaaaat caaaaaatag gtgggcaaac aataataaac attttaatag tagatggtag
     9481 caagaccaac acaggcggat tgaacattta agagttgata aaaaagaaaa atatcgaagt
     9541 atttgattag caactacttg tattcatttt tctattctta cgtggtatct ttgaataaag
     9601 cgaatcaata agaaagagat ggaagcagat gctaacgaaa aaagaactaa tcgatcacat
     9661 caatgaaaca tacaatttgt ctccagaata tatttttaaa aaatttcctg attattgcgt
     9721 attcagacat caaaaaagca aaaaatggtt tggggtcctg atgtctgttc cagaagaaaa
     9781 aatctatgat gatgcaagtg accataggat catcaatatt cttgatttga aggtcgatcc
     9841 tgaacttggc ggaattttgc gaggaaaaga ggggtatttt ccagcctatc acatgaataa
     9901 agaacattgg atctctattg atctacgtaa aatagaaaag ctagatgaac tagcagattt
     9961 gattgatgga agttttaaat caacgcaatg aatgtctagg acaatagaga agaggttgtg
    10021 acagaaatgt ttagcttcaa gaaataagaa ggaatttacg aaaattgctt ctcaaatttt
    10081 tgggaaattt cggcttattt tctaagaaat tacttctgga acaccgtttg ttcgaaaaaa
    10141 gagctgcgac aagaattttg tcccagctct ttttttttta ttttcctaaa taaaattccc
    10201 gcatcgtttt tgcaatcatt ttgatcaaag gatgatcttc tgtgaagcta gaagataaat
    10261 aaattgttcg tgtcaagggc aaatctttga tttcccgcaa gacgaaagga tcatttttat
    10321 cgtaagacca agaaactttt gggaaaaagc taacagctaa tccttgcttg acaatagaac
    10381 gtaatgtcgc aggatcatct gtcgtcgaac tgatcgtcaa ttcgatactt ttcgcttcaa
    10441 gtgctgcatc cagtgtatga cggaaggcgt tgctctttga cagcatcaag atgggggtgc
    10501 gttggatatc ttctaattgc agatgttctt ttttagcaag aggatgtttc ttaggaatgg
    10561 cgacataaac aggttctttc atgataggaa tattggtcat gccaggcttc atctccgaag
    10621 tgatgtacag tacaggatcg gtaatatcat ctggtaaatg atgttgcatg atcgttaatt
    10681 ggatatcagg atgcttttcc ataaaaattc ggaccaagtt cggaatagaa acagaggcta
    10741 cctctaaata caatatgacg gaagtaacga tcttctcatt gaccgaagac aataatttga
    10801 ttccttcttc taatgtgtct aatgtcttga ctgcatatgg ataaaattct tctgcataaa
    10861 cggtcgtctc aatgtttctg ccgctatggt aaaacaaagg gacacctaat tcgttttcca
    10921 aatgtttgat cgcacggctc aaggcaggtt gagaaataaa taattctttg gctgcttggg
    10981 taacattttt tgtttcgaat acttttttaa aatactgcag atgaagcaat ttcataatta
    11041 tccatcccat ccataccttt ttgttatggt tttttctttt tttggcattt tttattatag
    11101 cagaaattga ctaagatagc agtggaaaaa gtaagtgctg aggaggcgag gctcatgagt
    11161 acaggtttct tatttcctgg acaaggcagt cagaagttag ggatgttagc ggatatccct
    11221 gaaatgtaca tggaacgttt agaaaatgtg acggggtatc ggttagttga taaagaagaa
    11281 aactatcaag atacggtttt tatccagcta gcattactga caaaagcagt attttattta
    11341 gatgaaatgg aaaaaaacgg tattcagcct gatattgcag caggacattc gattggtgct
    11401 ttttgtgcag ctgttgcttg tcgatctttg agatttgaga atgcagcgga actagtctat
    11461 caccgcgcag tcttaatgaa agaagcttat ccaagcggtt atgcaatggg cgtagtagta
    11521 ggtgtgacga gaagcgaagc tgaggaaatt gtagcaaaaa catttgatcc tgaccatcca
    11581 gtttatctgt caaatgaaaa ttgtccgatg caacatacgt tatctgggag tatcgaaggt
    11641 ttatccgtta cgttggcaga agccaaacgg caccaagcgc aaacagcgaa gctgctgaaa
    11701 gtccctgtgc catctcactg tctcttgatg aatgatacgg tcaagaaatt caagccgtat
    11761 atgcagtctg tccatctatc tgatgctaga tgtttgtata taaaaaatac ggatggaaga
    11821 gccactcagg acgcagaaga gattcgtaaa gatttgttgg aaaacctagc tttccctgtg
    11881 cagtggaacc aaatgatgga cttggcaaaa gaattaggaa tggatctgac gattgaattt
    11941 ccccccggca atactttgac aaagctgatt catacaaaat ttggtgtaga taaagggatc
    12001 cgtacgatca atctagacca acatggtatt gatgacgcca cgtttctata tcaaaaatgg
    12061 agatgactaa ttatggaaaa accaaaccgc aactgggctt ctaaacgcga aaaaaaagcg
    12121 cagcgattgg aaaaaatcag caacttgctc aatggcaagt atgtagaaac tgaaaaaatc
    12181 gtagaagtaa tggaaaaact gattgctccc ggcgacaaag ttgtattaga aggcgacaat
    12241 caaaaacaag caagttttct ttcagaaagt cttgaaaaag tagatgcaga aaaagtgaat
    12301 gatctgcatt tgatcatgtc tagtatctca cgaccagaac atttgaatat ttttgaaaaa
    12361 ggaatcgcaa gcaagatcga tttctcttat gctggtgcac aaagcttgcg tgtcgcccaa
    12421 atgattgaag acggcacaat gaaattaggt gatatccata cttatttgga actttatgga
    12481 cgcttattca tcgatttgat tccaaatgtt gttttagtag ctgcagataa agcagataaa
    12541 gacggcaact tgtacacagg tttcaatacg gaagaaacac caacaatcat cgaagctgca
    12601 gctttcaaag atggtatcgt gatcgttcaa gttaacgaag ttgtcgattc tttaccacgt
    12661 gtagatatcc ctggtgactg ggtagacgtt gtcgtgaaag cagataaacc ttatcaatta
    12721 gaagctttat tcacacgaga tcctcaaaac atcacagaat tgcaaattct aatggcaatg
    12781 atggcaatca aaggaatcta cgcagaacac ggcgtacaat ctttgaatca cggtattgga
    12841 ttcaatacag cagctatcga attgttactg ccaacttatg gtgaatcact agggttaaaa
    12901 gggaaaatcg cgaagaactg ggtattgaac ccacatccaa cgatgattcc agccatcgaa
    12961 tcaggttggg ttgaaagcat ccatagtttt ggtggcgaag ttggtatgga aaaatatatt
    13021 gctgctcgcc cagatgtctt ttttgtcggt aaagacggca cgatgcgcag taaccgaaca
    13081 ttatcacaag ctgcaggaca atatgccatc gatatgttta tcggttcaac cttgcaatta
    13141 gattacgaag gaaactcttc taccgtaaca aaaggacgtt tatctggttt tggcggtgcg
    13201 cctaacatgg gacataatcc tggcggtcgt cgtcattcta caccagcatg gcaatcactt
    13261 cgcgacaatg atgatccatt agggaaaggc caaaaattag ttgttcaaat ggttgaaaca
    13321 tatggttcaa ataaaaaacc agtctttgtc gaagaattag atgcactagc tgttcaaaaa
    13381 caagcaggct tagccaatgc accagtgatg atttatagcg aagatactac acatatcgtg
    13441 acagaagaag gaattgctta tttgtacaaa gcaaaaacaa aagaagaacg acaagcagca
    13501 attgcagcaa ttggcggtgt tacacctctt ggaatgacta gcacgaaaga atcactagat
    13561 agcttgcgtg cagccggcat cgtgaaatta ccagaagatc taggcatcaa acgtagtgaa
    13621 gcgaaacgtt cattattagc tgcacaaacc atcgatgatt tgatcgaatg gtcagatggc
    13681 ctgtatgaac caccaatgaa attcagaact tggtcataaa attggcgaga aaaacctttt
    13741 gtcgccattt acccagatgg aagaagaaag acgaataagc agaacgtgta tgctctgttt
    13801 tacaaaaagg ggagagatcc atggaacaat tagcttataa atatccaacg aaacagccga
    13861 tcaataagaa aatccatgta ggagtggtcg gttcaggaga tttggaaatc ttgatgtttc
    13921 caacagaaaa aacagaatct actgtaaaaa tttgtacagg aagcgatggc ttcggcgaag
    13981 tatggaacaa tgtattaacg agattttttg atcgttatcc aatttcagca gatatcgtga
    14041 tcaatgactt cggtgctaca ccaggagttg tgtacctacg attgacgcaa gcaatggagg
    14101 aactttcaga tgaaaaatag ttttgtagaa ttaaacgcca gagaacgagt gaacgctcta
    14161 cttgacaatg gacgtgaatt gattgatcca ttcgatcaaa tcatcgcgcc aagtttacca
    14221 gcacaaggaa tcgtccctga aagtgatgac gggatcgttg tcagccgtgg cacgatcaat
    14281 ggcaaacaag cagtagtaat cgctatggaa ggaaaattcc aaggtggcgg tatcggtgaa
    14341 gtttccggtg caaaaatcgt tgctgcactt gaacatgtgt tagaagaaaa taaaaaagga
    14401 aatgagatct ttccagtcat tgtactagat acaggtggtg ttcgtttgca agaagcaaac
    14461 tacggactgt tatctatttc agaaatcgga aatgtaatca ttgctctaaa acaatatgta
    14521 ccagtcatcg gtttgattcc aggacgtgta ggatcatttg gcggtatgtc gattacttca
    14581 gcattaatga gttatttaat cgcaacaaaa aaagcgagag tcggtttgaa tggaccagaa
    14641 gttattgaac aagaagcagg cgttcgagaa tttgattcta gtgacaaaga cttgatttgg
    14701 aatacattag gatctcgtca acgggtagaa tcaaaaatca ttgatgaatt agtgactgat
    14761 agtgtagaag caatcaaagc tgctgtcatc gcagcaatgg acgaacgaaa agacacgcat
    14821 cgcagtgaaa gctcagcatt ttatttatca ttacttgatt ctttagatct ttcaaaacca
    14881 atggccattg acgattacaa tgaagcaatt gctaaccatc aagcaaaatc aattgactta
    14941 ccagaagtag cagaaggaaa tgaaccagct gcagcaagtg ttggttacga ttggtttgcg
    15001 gcactaactg gcatgaaaaa tccaacttct tctattagta ctgtgtatta tggtaaatca
    15061 agtaaattca tagaagaagc catcgtgaca tcaatcgtac ctgatcctaa aaacccgatg
    15121 taccgtgtac gaaacggtga agttggttta gttgaaggtt tccgcatggg acaaattttg
    15181 gatcacgttt acgaagaaga taaagacaaa aaagtgaaac ggccaatcat cgttgtgatc
    15241 gatgtaccaa gccaagcata cggctataac gaagaattga tcggtatcca tgtggctttg
    15301 gctaatagtg ctgctgctta tgcaaaactc cgtcaagctg gacatccagt tattggattg
    15361 attgcaggaa atgctatttc aggtgcattt ttagcacatg gattacaatc aagccgcttg
    15421 attgctttaa acaataaaga aattaccgtc caagcgatgt ctaaagaaag tgctgcacgt
    15481 gtgacaaaac gttcggttgc tgaaatcgaa gcagctgctg aaaaagttcc agctatcgct
    15541 tacgatatca ataattatac aaaactagga gcgatctatc atttcctaga agatatcacc
    15601 gaccaagctg cttcagaaca aaatgtcagc aaagtcatcg aagcaatcaa tgaagccatt
    15661 caagatgtcc gcaatacaaa tgacacgatg ctgacaaatc gatacacaaa cgaaacagcc
    15721 aaaacattcg gacgtgtcga aacaaataaa gtccgtgcca aaatggacga agaatgggat
    15781 gcataaaaaa tgattcaagc acatgatatt gtcctttttg ataaacatga aatagaaggg
    15841 ttcaccttgc ctgaatgggt cgttctaact gaaaagatgt tcggaacagt tcgtcgtgcg
    15901 tcgacggatc acgcagaaat actaccgatt ggtttacgtg gacctgcccg caatcagaga
    15961 tggtcaacag aaatctcaaa acatgccgtc aaacaagtca ttcatccgtg ggacatcatc
    16021 gaacaagaat cgtatagaca agagaaaatc atggattatc ctgtttatca gcaatttcat
    16081 gctgctagaa acatattatc aggatgtaaa tggggaatcg gcggcagctt aggatttgaa
    16141 ttatcgactg ggattccggc agtaaaagaa accagtgatt ttgatttgct gctgtacgca
    16201 aattcaccga ttgaattgcc aattcaggta attcaatcgc atcctgcttt ttttgagcaa
    16261 ttcgataccc aagtagtgac tcggcaaggc ggtttttcac taaaagagta tttgagagag
    16321 ccagagaaaa aaattctatt gaaaacagtc gaaggaccta aattaaccaa agaactttgg
    16381 taataaagaa aaagagagag aaagagatgt ttgaaaacaa aaaaatcaaa atcaacgtaa
    16441 gtgacttcca gaaagtatca gctgtaacgg ctgttatgat gcaaacgata ttgagctttg
    16501 ctttggcaaa cagccacagc gcaacaaata cagcttttct tggtaccttt tacgtcatcg
    16561 ttaaatacac agcaccaatg tttatttttg caattgttta taatatggta aaaacaagtc
    16621 agcatttgtc ttattgggaa tttttgaaag aaaagttttt tgaactagtc gttccgtatc
    16681 ttctatggac gacggcttat ctttggctat ttccagcagt ccaacaacaa actccgtata
    16741 cggatactgc aagcttttta ctgaaatatg tcacaggtga tggagcgcct catttatggt
    16801 ataccgttat gatgctgcag atccaattgt tcatgccgtt ttttgtttgg ctcggttaca
    16861 aagtatttgc taataaaaaa tatgtatggc cagtattgat tggttcaacg attttatatg
    16921 tcgcatggta tctgttctat caaacacaag tattgactgg gccttatcat gacagctggt
    16981 acttattaga tcggtttgtt gctagtttca tgatttatgg ggtagccgca tttgtttatc
    17041 acgaaaaagt ctatcaatat ttggacagag tacgttatct tttcttacct gttgccttag
    17101 ttatcgcgtt cttttctgtc cgttcattgc tcgcacatcc aggagacttg agctttgcga
    17161 atgcacctta cttgaatacg atccaaagtt tatacagttt agtcatcatc tttgctgtct
    17221 tcataggcgc ttccaaaatg attgtaaacg attcgccaaa attgccattg ttcaaatggt
    17281 tgtctgttta tgcttatcgt acgtatttag ccaatgtatt tgttttccaa gtactgttat
    17341 tattgtttaa agatagttgg ctgcagttac cttcaggagt catgattctt gtagcttatc
    17401 taatgacggc aagttgtgca tttgctttaa gctggttact tcatatcatt tgggtagcta
    17461 tcaaaaaagg tttcagcaag taatagtagg aagaaggagt gagtgtcatg ataaatgacg
    17521 aacgaaaaaa agcagtgact cagcaactcg ctgcgttggt agaggaagca ttgatcgcag
    17581 aagtaacatt aagtccaaaa cctggattag tggatgcact ggatgcgggt gcacattccg
    17641 atatggacta tgctttattt cttaagtctg ctaagacgct aactcctttt tttgaagaaa
    17701 tggctcaaat agcatggcat catgctattg atcaggagtt aagagaaaga attgctgaga
    17761 ttgggcgaaa agcggaaaag gcaatgctga ttgctacaaa tggcatcaat acccataaag
    17821 gagcgatttg ggcaatgggg ctattgatca gtgtcattgc acaaaaaatc agtatggaac
    17881 agcctttttc tttatcgaaa atacttgatg atgtcagcaa gcttgctgct tttcctgata
    17941 caaaatatcg tcataaaaaa accactcatg gtcatagggt gaagcaaaaa tatggcgtaa
    18001 atggtgcata tgaagaagcg atattagggt atccgcatat caggcttgca attcaagcag
    18061 ctaaggagct agataatgat tcatatgtac agaaacaaat gcatatgttg ttagttttga
    18121 tgagttcgct agatgacaca tgcgtattgc atcgcagtga tcagaaaacg ttagtggaaa
    18181 tgcaaaaact tgctcatgct gctagtcaaa gtgctttacc aaatcgtcca tttacagcac
    18241 tgttgcactt ttgtcaggaa aaccaaatct ctcctggagg aagcgcagat ctcttgtctg
    18301 caagcttgtt catgctgaag atagaggaat tatggactgc cgacacatac atttgtccga
    18361 tgttactcaa tgactaaata agacaaaatt gctaaaaaaa cagtttatgc atctggttat
    18421 tgactcttcc ttttagcaag gctatcataa aaatagtttt ttgtaataaa agataacgag
    18481 gaagacaatg aaaaaaatca attatttagc aatcattttg ttagtgggcc tatgtttgtt
    18541 tgctctgccg acccaaggat tcgctgtcgc gggtggtcat ggtggaggta catctagcgg
    18601 tcgaagttcg ggcagaggtt catttggtgg tcgttcatat gggggcagtt attttggcag
    18661 tcgttcttac ggcagagatt cttatgggta ttatggtcca ggtacaggtc cttctagtta
    18721 tgagcaaacg ggatttcttg cattagctgt tttgtcagta ggaggggttt ctgctttttc
    18781 tatcgtcaag aataaaagac aaaagaaaag gctgaaagcg gagatattgt cccgcatgtc
    18841 aggaacggct aaagaaaaga aagcacagtt tctggaaatc caaaagatat tcatgaagat
    18901 ccaatccgct tgggaagaag aaactcctga aaaagccaga agctgttata cagacaagct
    18961 gtttgccgaa catcagaaag ttctgcaaaa aaacaaagca gacggtgtaa gaaatcatac
    19021 taaaaaaata actattcagg aagtaggaaa ttatcgacag atccacgaaa atagttttgc
    19081 catccgaatt gatttcagat gctgtgatta ttcagtcgat cgaaaagatg ggcgattgat
    19141 ctcaggaagt aagacacaac gacagtattt ttctcagata tggtattttg atttttctga
    19201 gaaagaaaaa agatggcaag ccgattttat tcagccgatt atgctggatt gacaggaaat
    19261 atttatgaac ggagttgtga caaaagcctt gtcgcagctc ctttttctga ataaacggtg
    19321 tgccagagat agcctatcgg caataaggca cgattttgta aaatatggga agcgattttt
    19381 caaaatcgct tcttatcgct caaggctgaa catctctgtc acaacctctc cactaaatgg
    19441 tgtttaaaag cttgcatctg cgataataag aacaaccaaa cgaaaaatag gaagcactat
    19501 tttagtttag ttgttcttat tacttgatgc aggacagctt tttcacaacc tcttttttgt
    19561 atagatgggc aaaatttttg taataatagg cgtatgtctt tttcgttgag gagatgaaaa
    19621 agcatatggc ttattttttg tttgctttgc ttactttctt tccacttcaa gtggcagaaa
    19681 tatctacttt ttattatgta agcggaacca tatacttgaa atcagaagga gatcatttct
    19741 atgaaaacaa gaacaattga aatacttcac agaatcatga cagcccaata tccagtaaaa
    19801 gcaaaacatc tagcaagtga gttcaatgtc agtcagcgga cgatccgtca ggaagttctt
    19861 gaagcgaata tctggttgag cagtaaaaag cttccggaga tccgtacaat tcgcaacaaa
    19921 ggttttttgt tgaagctgga aaaggaaaag caagctcgat tagaaactga aataaaagat
    19981 gtgaaagacg gctttttgaa tcggcgcgaa cgtatttttg atctagtttt agccgttgct
    20041 tacagccgaa tcccgatcca tctaaacaaa aaagaagaag aatttcaaat ctctaaaagt
    20101 acattagatg aagatatgag acggttacga gcaacactgg tcagttatgg gatagagatc
    20161 gtcagttatg gaaagcaagg actggtttat aaaggttcag aacgatctat ccgtacgatg
    20221 atctatgatt ttatcaatca gaatctggga atgaccgact tctttatgaa tcaagatgca
    20281 caattaacac cggctcaaaa aatctttctc cattattttc ctttggtgga aatcaaaaaa
    20341 atacaggaga tctattccaa taagttgttg aaacaagaag atgatattta taaaaaacaa
    20401 atcctgcttt ttacattgat ctggatccag cgaatcagga aacatgaatt tatctctgcc
    20461 attaattgga aaaatacaga aataaaagac agtagctttt ctgcatttat tgaccacatg
    20521 atcgaggaat ttcaattgga caatgtcccg caagtagaaa gaaattatct tcgtttcact
    20581 attgagacgt tcaatgctag agatatctcc aattcaatcg agtgggtaca agctcaatta
    20641 ctaacgatcc aattgatcca atttgtagag aacgagacac acgtaccttt ccatctaaag
    20701 gaagaagctt tattcgaaaa tctctataag cacatggctg cactgattgt aagaatcaag
    20761 aatcatatcc aagtcatgaa tccattgaaa gaaaacatcc gaacaaatta tttcccaatc
    20821 taccgcgcag tcactcattt tgttcctacg atcgaagaag taattggtgg caagatactg
    20881 gaggacgaga tagcttttct tgtcatccac ttttcgacga tcgccagtgt aatcaagcga
    20941 gacttgaatt atgtttataa atcagtcgtt gtctgtaacc atgggatggc tactggaaat
    21001 ctgttagcag agaatctgaa agaaaagttt cctcagatcg agattactgc agttttaagt
    21061 tcaaaagaaa tcgaattagt agataaattg gatgtcaatc tggttttcag cacctttcaa
    21121 ttgaattatc gaaataagcc gcttttagtt gtcgatccga ttttaactga gacgaatatc
    21181 ttggttatag aagacttttt gaaaagaaat gaagaactac agcggttaga gcctaacagt
    21241 catgaatcga cggagttatt cactcaaata atagatgtca tgaaagaaag tggtggccga
    21301 gtagatcgaa tcatctatgg caagttagaa aaattattcg aaacaaacaa tttagaaatc
    21361 aacaaaaggg agatccagcc tatgttaaaa gatattttga cggacagcaa tatcatgatc
    21421 aaagaaaaag ccactacttg ggaggaatcg attgaactag ctgctagacc attggttaaa
    21481 gaaaacattg tcgaagagcg ctatgtcgaa gcgatgattc gtgcagtgaa tgattacgga
    21541 ccgtatatcg tgattggcaa acacatggca ctagcccatg cccgaccaga agatggggtc
    21601 aataaattag gagtcagcgt agctacgatt gagcaaccaa tcgatttcgg taatcctgaa
    21661 atggatccag taaagattat tttttgtttg gcagcagtag actcctactc gcatctgaac
    21721 atcatgaaag aactaattga attgatcaac aacgaagaga agttggatcg attgattgaa
    21781 tgcacaaata tcgaagattt caaacaactg cttttttcag tagttgagaa ataataaatg
    21841 atacggggga gaacaaagat gaacaaattg aacattttat tcgtatgcgg tgcaggttta
    21901 ggaagtagtt ttgcagcaca gatgtctgct gaggatgttt tgaacaaatt aggagtagaa
    21961 gccaaactgg accattgtga tatttcttcc gcagtttcca tgaaaccaga tgtgatcatc
    22021 acagcagaaa attttcgttc gcagttcgaa aagttcgcag ttgatcctaa aacggcgatg
    22081 atctatttga aaaatattgt ttcaaaaaat gaaattgaag aaaaaatcac cccagttctg
    22141 caagataagg gagttatcta aatacaaaaa gaaaagaggg taaaaa
//
LOCUS       DAZKSU010000044        21833 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000044 DAZKSU010000000
VERSION     DAZKSU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.044"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG8Z3_001718"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG8Z3_001718"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0824027.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG8Z3_001719"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG8Z3_001719"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0824028.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG8Z3_001720"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG8Z3_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0824029.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG8Z3_001721"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG8Z3_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0824030.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG8Z3_001722"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG8Z3_001722"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0824031.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG8Z3_001723"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG8Z3_001723"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0824032.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACG8Z3_001724"
     CDS             complement(8054..8524)
                     /locus_tag="ACG8Z3_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0824033.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACG8Z3_001725"
     CDS             complement(8570..8995)
                     /locus_tag="ACG8Z3_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0824034.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACG8Z3_001726"
     CDS             complement(9062..9331)
                     /locus_tag="ACG8Z3_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0824035.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACG8Z3_001727"
     CDS             complement(9342..10937)
                     /locus_tag="ACG8Z3_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0824036.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG8Z3_001728"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG8Z3_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0824037.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG8Z3_001729"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG8Z3_001729"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0824038.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="ACG8Z3_001730"
     CDS             15403..16377
                     /locus_tag="ACG8Z3_001730"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0824039.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="ACG8Z3_001731"
     CDS             16547..17947
                     /locus_tag="ACG8Z3_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0824040.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="ACG8Z3_001732"
     CDS             complement(18025..18747)
                     /locus_tag="ACG8Z3_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0824041.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19332)
                     /locus_tag="ACG8Z3_001733"
     CDS             complement(18826..19332)
                     /locus_tag="ACG8Z3_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0824042.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19455..21254
                     /locus_tag="ACG8Z3_001734"
     CDS             19455..21254
                     /locus_tag="ACG8Z3_001734"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0824043.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21322..>21833)
                     /locus_tag="ACG8Z3_001735"
     CDS             complement(21322..>21833)
                     /locus_tag="ACG8Z3_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0824044.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6048 a         4789 c         3965 g         7031 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt
    19321 tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat
    19381 cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg
    19441 aagggagttt tttcatgagt tattcattaa attggaatct agattctatt ttttccggag
    19501 gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt
    19561 attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta
    19621 ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg
    19681 cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg
    19741 caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat
    19801 ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt
    19861 taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa
    19921 atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca
    19981 gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct
    20041 tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg
    20101 aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt
    20161 ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt
    20221 acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac
    20281 aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat
    20341 ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg
    20401 acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat
    20461 ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc
    20521 ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca
    20581 gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca
    20641 gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag
    20701 caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag
    20761 aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata
    20821 tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct
    20881 cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt
    20941 tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac
    21001 cgttttataa cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg
    21061 ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct
    21121 caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt
    21181 tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag
    21241 aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta
    21301 actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac
    21361 tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa
    21421 agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt
    21481 tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat
    21541 gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact
    21601 tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat
    21661 tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc
    21721 aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac
    21781 actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttg
//
LOCUS       DAZKSU010000045        21344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000045 DAZKSU010000000
VERSION     DAZKSU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.045"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            272..973
                     /locus_tag="ACG8Z3_001736"
     CDS             272..973
                     /locus_tag="ACG8Z3_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0824045.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1545..2561)
                     /gene="tsaD"
                     /locus_tag="ACG8Z3_001737"
     CDS             complement(1545..2561)
                     /gene="tsaD"
                     /locus_tag="ACG8Z3_001737"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0824046.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2558..3022)
                     /gene="rimI"
                     /locus_tag="ACG8Z3_001738"
     CDS             complement(2558..3022)
                     /gene="rimI"
                     /locus_tag="ACG8Z3_001738"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0824047.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(3029..3571)
                     /gene="rimI"
                     /locus_tag="ACG8Z3_001739"
     CDS             complement(3029..3571)
                     /gene="rimI"
                     /locus_tag="ACG8Z3_001739"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0824048.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3555..4277)
                     /gene="tsaB"
                     /locus_tag="ACG8Z3_001740"
     CDS             complement(3555..4277)
                     /gene="tsaB"
                     /locus_tag="ACG8Z3_001740"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0824049.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4468..5448)
                     /locus_tag="ACG8Z3_001741"
     CDS             complement(4468..5448)
                     /locus_tag="ACG8Z3_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0824050.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5472..6956)
                     /gene="galT"
                     /locus_tag="ACG8Z3_001742"
     CDS             complement(5472..6956)
                     /gene="galT"
                     /locus_tag="ACG8Z3_001742"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0824051.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6968..7957)
                     /gene="galE"
                     /locus_tag="ACG8Z3_001743"
     CDS             complement(6968..7957)
                     /gene="galE"
                     /locus_tag="ACG8Z3_001743"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0824052.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8206..8373
                     /locus_tag="ACG8Z3_001744"
     CDS             8206..8373
                     /locus_tag="ACG8Z3_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0824053.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8435..10246)
                     /locus_tag="ACG8Z3_001745"
     CDS             complement(8435..10246)
                     /locus_tag="ACG8Z3_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0824054.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10243..10608)
                     /locus_tag="ACG8Z3_001746"
     CDS             complement(10243..10608)
                     /locus_tag="ACG8Z3_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0824055.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10771..11166)
                     /locus_tag="ACG8Z3_001747"
     CDS             complement(10771..11166)
                     /locus_tag="ACG8Z3_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0824056.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11184..12146)
                     /locus_tag="ACG8Z3_001748"
     CDS             complement(11184..12146)
                     /locus_tag="ACG8Z3_001748"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0824057.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12146..12358)
                     /locus_tag="ACG8Z3_001749"
     CDS             complement(12146..12358)
                     /locus_tag="ACG8Z3_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0824058.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12379..13077)
                     /locus_tag="ACG8Z3_001750"
     CDS             complement(12379..13077)
                     /locus_tag="ACG8Z3_001750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0824059.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13097..13639)
                     /locus_tag="ACG8Z3_001751"
     CDS             complement(13097..13639)
                     /locus_tag="ACG8Z3_001751"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0824060.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13771..14775)
                     /locus_tag="ACG8Z3_001752"
     CDS             complement(13771..14775)
                     /locus_tag="ACG8Z3_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0824061.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14772..15761)
                     /locus_tag="ACG8Z3_001753"
     CDS             complement(14772..15761)
                     /locus_tag="ACG8Z3_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0824062.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15758..16564)
                     /locus_tag="ACG8Z3_001754"
     CDS             complement(15758..16564)
                     /locus_tag="ACG8Z3_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824063.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16730..17686
                     /locus_tag="ACG8Z3_001755"
     CDS             16730..17686
                     /locus_tag="ACG8Z3_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0824064.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17763..18281)
                     /locus_tag="ACG8Z3_001756"
     CDS             complement(17763..18281)
                     /locus_tag="ACG8Z3_001756"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0824065.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18369..18518)
                     /gene="rpmG"
                     /locus_tag="ACG8Z3_001757"
     CDS             complement(18369..18518)
                     /gene="rpmG"
                     /locus_tag="ACG8Z3_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0824066.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18746..19192
                     /locus_tag="ACG8Z3_001758"
     CDS             18746..19192
                     /locus_tag="ACG8Z3_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0824067.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19387..21282)
                     /gene="asnB"
                     /locus_tag="ACG8Z3_001759"
     CDS             complement(19387..21282)
                     /gene="asnB"
                     /locus_tag="ACG8Z3_001759"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0824068.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6420 a         4526 c         3678 g         6720 t
ORIGIN      
        1 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
       61 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
      121 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
      181 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
      241 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
      301 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
      361 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
      421 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
      481 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
      541 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
      601 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
      661 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
      721 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
      781 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
      841 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
      901 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
      961 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
     1021 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
     1081 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
     1141 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
     1201 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
     1261 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
     1321 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
     1381 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
     1441 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
     1501 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
     1561 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
     1621 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
     1681 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
     1741 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
     1801 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
     1861 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
     1921 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
     1981 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
     2041 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
     2101 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
     2161 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
     2221 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
     2281 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
     2341 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
     2401 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
     2461 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
     2521 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
     2581 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
     2641 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
     2701 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
     2761 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
     2821 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
     2881 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
     2941 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
     3001 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
     3061 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
     3121 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
     3181 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
     3241 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
     3301 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
     3361 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
     3421 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
     3481 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
     3541 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
     3601 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
     3661 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
     3721 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
     3781 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
     3841 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
     3901 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
     3961 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
     4021 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
     4081 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
     4141 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
     4201 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
     4261 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
     4321 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
     4381 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
     4441 ttcatttccc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
     4501 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
     4561 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagag agggcgttac
     4621 atatttcgct acactgacat cattgaaacc catcacagaa atatcttctg gtaccactaa
     4681 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
     4741 cgcatcagga acatgcggat tcgtttttaa atactgcttc actgcttgat atcctgactc
     4801 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
     4861 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
     4921 tccagcaagc atagcgatat gttgcgcttt atgggagaca aaatgttcaa tgactttttg
     4981 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
     5041 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
     5101 tcctagtgcg atcgttccat ctgtcggaag atcactaatg gaatcccacg tttctttcaa
     5161 taaagtaacg tttgcttctt ctgccttttt ttcgattcct agacgaatag ataaataata
     5221 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
     5281 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
     5341 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
     5401 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
     5461 cttttttacg tttataaaat tgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
     5521 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
     5581 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
     5641 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
     5701 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
     5761 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
     5821 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
     5881 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
     5941 actgtttcat ccgaatatgc tttccacttt tccaatactg agttagctgc ttcaatcaac
     6001 aattttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
     6061 atgacttgcg gataatctgc tagtttgata agtttttcta tcggtgcttt tgccatagga
     6121 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaatcggc
     6181 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
     6241 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
     6301 tcattgtaat aagcataagg agaatattgg aatccccagc tttcaccgtc tagattcatt
     6361 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
     6421 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
     6481 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
     6541 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
     6601 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
     6661 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
     6721 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
     6781 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
     6841 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
     6901 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
     6961 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
     7021 taattgcttt gatatctgtg tattcaggtt tccaacccag aacctctctc gctttttcac
     7081 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
     7141 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
     7201 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
     7261 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
     7321 cataatcgtc tccataaaca gccaatgctt ttctttgacc aagagctact tgtaaaatga
     7381 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
     7441 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
     7501 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
     7561 tatcttctgt gattggagac tcttttggtt ctccgtaagt tgctgcagta gaagagaaca
     7621 cgattttatt cacattgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
     7681 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
     7741 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
     7801 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
     7861 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
     7921 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
     7981 aaattgtgtt tgttgattat atataattta aaaaaacgaa gtttactttt atggtgaaaa
     8041 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
     8101 attttcaagg attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
     8161 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
     8221 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggtcgcttct
     8281 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
     8341 aaaaaagcaa tgcgcaaacg cgtttctcgt taataattat ttaaaaacac gcatgaagac
     8401 ttgtgaaaga acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
     8461 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
     8521 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
     8581 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
     8641 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
     8701 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
     8761 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
     8821 caaatggcgg ttcgaacaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
     8881 tccgaatgac tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
     8941 gtatttcttt gtattccttt gcagaaaaaa cgtgccattc aatagcctgc cggattggat
     9001 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
     9061 ccgcttcttt tatctgccct tcaataaatg ctcggctagc ttctgcaaag gactcatcct
     9121 gataggtaag aggaatttct aaatacgacc aatcccatct ataagggatt agtgctggat
     9181 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
     9241 gaatcggcaa atgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
     9301 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
     9361 tttcttttgt tagtaaccgt ggccatgcca ttgatccgcc ttgcttttgg tttcttcctc
     9421 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
     9481 gatgatagac aagttttcct ttttcgttct cgaagcgccc ttctcttgat actgtaaaca
     9541 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
     9601 gtatcttcga aagatcgaca tctgcaggat tagaaggaag ctgatacagt aaatcatttt
     9661 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
     9721 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
     9781 cttttctttt gctgactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
     9841 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
     9901 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
     9961 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
    10021 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
    10081 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
    10141 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    10201 ctatacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    10261 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    10321 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    10381 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    10441 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    10501 taactgcgct ttttcttttg aaattcctcg tggccagaac ctatccacga gtattctata
    10561 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    10621 ctttctgcct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    10681 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    10741 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    10801 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    10861 atttgctgta cggatgctca agcttttctt ttgatcgtaa atatagacag gctggtcttt
    10921 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    10981 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    11041 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgc ttggccatta ctcgtagata
    11101 gacttcccaa aagatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    11161 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    11221 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    11281 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    11341 actcctccac ctaacacaat acttgacgga ttcaatgtat tccccaagtt ggcgcaagct
    11401 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    11461 gccatttcaa agatatcttt acttgttact tcttcaccgt tgtctaacat tgctttcagt
    11521 tttgaatctc ctgcgtattc ttctgccaag taacgaccta gacgtaccac accagtagcg
    11581 ctagacacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    11641 acagtgatat ggccgatctc tccgcctgct ccgctcacgc cgtgaatcaa atttccgcca
    11701 gcaacgatac caccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    11761 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcatcatt atcgatagcg
    11821 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    11881 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    11941 ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg gttgattgat
    12001 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    12061 ttttgttgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    12121 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    12181 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    12241 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    12301 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    12361 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    12421 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    12481 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    12541 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    12601 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    12661 taagataaac aacgcaccga acaaaccgaa tatcgaagta ctcgcaccac cagacagaac
    12721 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    12781 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    12841 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    12901 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    12961 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    13021 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    13081 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    13141 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    13201 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    13261 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    13321 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    13381 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    13441 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    13501 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    13561 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    13621 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    13681 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    13741 aaatgattgg ccttcctttt ttaataaatt tcaactgttc agcgcgtgtt tttgtctgat
    13801 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    13861 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    13921 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    13981 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    14041 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    14101 ttgcgcgcgt tcttgaccaa atgatagcaa tccaatcctc tgtgcataaa gtcctcctag
    14161 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    14221 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    14281 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    14341 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    14401 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    14461 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    14521 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    14581 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    14641 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    14701 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    14761 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    14821 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    14881 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    14941 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    15001 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    15061 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    15121 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    15181 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    15241 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    15301 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    15361 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    15421 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    15481 cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc
    15541 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    15601 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    15661 tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    15721 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    15781 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    15841 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    15901 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    15961 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    16021 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    16081 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    16141 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    16201 actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    16261 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    16321 ataatgtaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    16381 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    16441 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    16501 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    16561 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    16621 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    16681 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    16741 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    16801 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    16861 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    16921 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    16981 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    17041 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    17101 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    17161 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    17221 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    17281 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    17341 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    17401 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    17461 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    17521 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt
    17581 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    17641 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    17701 agaatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    17761 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    17821 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    17881 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    17941 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    18001 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    18061 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    18121 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    18181 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    18241 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    18301 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    18361 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    18421 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    18481 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    18541 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    18601 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    18661 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    18721 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    18781 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    18841 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    18901 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    18961 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    19021 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    19081 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    19141 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    19201 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    19261 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    19321 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    19381 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    19441 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    19501 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    19561 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    19621 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    19681 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    19741 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    19801 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    19861 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    19921 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    19981 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    20041 tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa
    20101 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    20161 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    20221 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    20281 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    20341 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    20401 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    20461 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    20521 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    20581 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    20641 gtctagtttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    20701 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    20761 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    20821 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    20881 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    20941 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    21001 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    21061 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    21121 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    21181 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    21241 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    21301 tataaaatta ctgtttattt cattcatatg catcttatca taga
//
LOCUS       DAZKSU010000046        20458 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000046 DAZKSU010000000
VERSION     DAZKSU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.046"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..778)
                     /locus_tag="ACG8Z3_001760"
     CDS             complement(164..778)
                     /locus_tag="ACG8Z3_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0824069.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            922..1484
                     /locus_tag="ACG8Z3_001761"
                     /pseudo
     CDS             922..1484
                     /locus_tag="ACG8Z3_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1595..2569)
                     /gene="lacD"
                     /locus_tag="ACG8Z3_001762"
     CDS             complement(1595..2569)
                     /gene="lacD"
                     /locus_tag="ACG8Z3_001762"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0824070.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(2588..3043)
                     /locus_tag="ACG8Z3_001763"
     CDS             complement(2588..3043)
                     /locus_tag="ACG8Z3_001763"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0824071.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(3071..4516)
                     /locus_tag="ACG8Z3_001764"
     CDS             complement(3071..4516)
                     /locus_tag="ACG8Z3_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0824072.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(4513..5448)
                     /gene="pfkB"
                     /locus_tag="ACG8Z3_001765"
     CDS             complement(4513..5448)
                     /gene="pfkB"
                     /locus_tag="ACG8Z3_001765"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0824073.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            5616..6374
                     /locus_tag="ACG8Z3_001766"
     CDS             5616..6374
                     /locus_tag="ACG8Z3_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0824074.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            complement(6869..8068)
                     /locus_tag="ACG8Z3_001767"
     CDS             complement(6869..8068)
                     /locus_tag="ACG8Z3_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0824075.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8511..9002)
                     /locus_tag="ACG8Z3_001768"
     CDS             complement(8511..9002)
                     /locus_tag="ACG8Z3_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824076.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            complement(9029..10174)
                     /gene="dgoD"
                     /locus_tag="ACG8Z3_001769"
     CDS             complement(9029..10174)
                     /gene="dgoD"
                     /locus_tag="ACG8Z3_001769"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFQ0824077.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            complement(10189..11556)
                     /locus_tag="ACG8Z3_001770"
     CDS             complement(10189..11556)
                     /locus_tag="ACG8Z3_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0824078.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            complement(11581..11859)
                     /locus_tag="ACG8Z3_001771"
     CDS             complement(11581..11859)
                     /locus_tag="ACG8Z3_001771"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0824079.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            complement(11878..12336)
                     /locus_tag="ACG8Z3_001772"
     CDS             complement(11878..12336)
                     /locus_tag="ACG8Z3_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0824080.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            complement(12348..12968)
                     /locus_tag="ACG8Z3_001773"
     CDS             complement(12348..12968)
                     /locus_tag="ACG8Z3_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0824081.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            complement(12979..15021)
                     /locus_tag="ACG8Z3_001774"
     CDS             complement(12979..15021)
                     /locus_tag="ACG8Z3_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0824082.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGKDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            complement(15399..16538)
                     /locus_tag="ACG8Z3_001775"
     CDS             complement(15399..16538)
                     /locus_tag="ACG8Z3_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0824083.1"
                     /translation="MNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGS
                     YDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTLAKE
                     AIHIVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAG
                     AVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFA
                     EKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQ
                     NEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            complement(16535..17119)
                     /locus_tag="ACG8Z3_001776"
     CDS             complement(16535..17119)
                     /locus_tag="ACG8Z3_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFQ0824084.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFISKKAI"
     gene            complement(17309..20341)
                     /locus_tag="ACG8Z3_001777"
     CDS             complement(17309..20341)
                     /locus_tag="ACG8Z3_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFQ0824085.1"
                     /translation="MVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDCQKL
                     LGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRFKVA
                     YFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKNQYT
                     GQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPFNQG
                     SNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDREVTK
                     KTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIHDQL
                     NYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGRKII
                     ITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEYYEL
                     EGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFEPFH
                     VYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLHPWA
                     VTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDLDVL
                     VAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGFDEP
                     LLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQTNL
                     REEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKPIMD
                     RYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKETRE
                     NIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEIIHKI
                     NERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDKVME
                     QYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
BASE COUNT         6208 a         4345 c         3393 g         6512 t
ORIGIN      
        1 tacaaaaata tttttattgt tggatacaga aatctatttt taactgggga tatctttgcc
       61 agctaatatc tttgagacta cacctatctt catctaaatg gatcaattac tcgctttttc
      121 cattgacctt cgtggtactt ctgctttctg atagtaaaaa acattactga atgatcttac
      181 tatataacat acagcggaga tctacttctt tattaaggat cttcatgacg ttatcttttt
      241 cttttttccg ttcgaaaccg cgtcgttcat aaaattgata cgtacagccg ctatccgtga
      301 acagaaatac ttcttttcct tgctctcggc gttcaaattc ttttaataat ttactaccga
      361 tccccttgat ctttaaatca gggttggcag ctaaaaagac aatttctcca tctggggaat
      421 gtttttttaa atagtctgaa agcaattcgt cattggtgtc gtcatacccg cctttgctgt
      481 ctttcacaaa tgcattttgt aagaaatcaa atagtttaac atataacttt tgactaaatg
      541 aatgatgctt ttttgatttt cctttgatgt ctgctaataa aacacctgcc aactcatcat
      601 ccgcatataa agcaatgatt tgtgtcgcgc gattcaattc tagataccaa aagtatcttc
      661 cataaagatt caaaaggagt ttgttcttgg tataccaatc aaagtgcata cctttaatcg
      721 cgaattgaat ggcttttttg tagtctttct tttccagttg cttaaaggct aattccatca
      781 cttattctcc tattctttaa atattgatat gctatactta tattataata gacaaggtgt
      841 cggttatcta tgttcagata gcggtgaaat tgtcggataa ccgacacaat tccttgatta
      901 tgttgattag gaggctgcaa aatgaaaaaa gaggatcgcc gcgtcaaaaa aacagaaaaa
      961 gcgttagcag aagcattatc aaaattatta gtagataaaa aaattcaaaa catcaccatc
     1021 cgtgaactga ccgaaactgc tgatcttcac cgttcgacat tttatacaca ttatacggat
     1081 atctatgatc tttatgaaaa actggaaaat agctttttcc aagacttgga tgaaatcatt
     1141 tcctacgata cgacacatag ctacgaggat ctatacgctc aattgatcga ctatgtctat
     1201 acgaatacgt tcgtatacaa gttattcgtc atcaaccacg gagatatcca atttcaaaag
     1261 agattaaata atctgatcga agaaaattat ttaaaaatct ggctacaaga agacaaaaaa
     1321 aaccgtgatc acagaagaaa tgcgttactt caccatctac catatccaag ggtgcctctc
     1381 cgttttaaat cattgggtag aaaagcaatt ttgcatactc taagaaaaaa atgctgacct
     1441 tcttgcgctt attgaacgac aacatagaac ctatcatgcc ttaaaaacaa acgaaaaagg
     1501 aagcgtcggg acgcttccaa ttctttattt aaaagctcaa attgattatt aaaaatcatt
     1561 aggttacaaa cttaaggtta tagttaaagc tttcttacaa tttaatagct gttgctgttt
     1621 ctttcaaaac attattcagc tcttcgatgt tcttgcgtcc ttgtgtctgc atccattctt
     1681 tggcagcata ttcaccttta gtaacgaatg gttctacacc attcgcccaa gttgcgcgcc
     1741 cacagagtac cccattgaat gtagagccag cctcttttgc aaatttcaag gtttcttgga
     1801 acaattcagc agttactcct gcgctcaaga aaataaatgg caggttggta gtctcggatt
     1861 gttctttgaa ataagcagca gcttcttctt tggtataagc aacttcattt ttagtgtaac
     1921 catccacata gttcatatta acaggaactt ccactttcaa aacgtcaact ccatagcgag
     1981 gcttagaaaa ttccttcatc atttcattta ctttatgcgg ttttttcaag gcatattcct
     2041 tcgaagaagt atcaggatcg ttggcatcat aagaaaccaa ttctaagaaa aatggcaaat
     2101 cttcagcaac acattcagaa ccaatccgct ccataaacgc cttcttttgg tcattgatgt
     2161 catcagattc atctacatcg tagtataaca agaacttaca agcatcggcc ccttgctcct
     2221 ttagacgttt tacagaccaa atagataaaa tatccggtaa acgtcctgga gttgaagcat
     2281 cgtaaccagt tttttcatag gccaacaaca gaccagcatt tttatcgcga acttcggctg
     2341 ccggcaaccc atattctgga tctaataaga ttgctgaagc atatggagtc aattcagaag
     2401 aaatgatttt tttgaaattg atgatttgtt catcggttgc tgatttattt tgaaatttac
     2461 cgatcatttt tttcaaggca ccacgttgat cgattgctaa cgcactgatg atgccacttt
     2521 cattacttag ttgacgaata taagttttct ttttttctgt taaaggcatg atttcactac
     2581 ttccttttta gttttcttct aatttttggt ttaaaagttc agctatttct tcagcagact
     2641 gggcttgttt cagaccttct gtcacatctt tgtgcatcaa aagtctggct acctgagata
     2701 aaaattttag atgggttgtt ccagcctctg aatctggaat aaacagagcg attacaaaat
     2761 ccgttccttc tccatctaaa cttttccaat cgaccttacc gtttaaccga acaatcacaa
     2821 tatcggcatc cttgatacta gcatctttgg catgagggat ggcaaaacca tccatcatac
     2881 cagtcgtgct ttcttcttct ctttccatca atttttcgta aactgcatct cggtcttcac
     2941 taatgctcaa gacaattgac tgatctgcaa ggaagttcat tacctcatct tgcgtcgtaa
     3001 aatttttatt cataaaaact ttatttgttt tgatcaaagt catttgaaat ccttctttct
     3061 agaataacct ttagttgttg gttgcttctt tcatttcaac agcagctgct tctgcttcac
     3121 gtttttctac actacgtttt tggatcaacc ctaaaattac tccgccaacg attgaaccaa
     3181 gtaaaatcga ccctacccat gcaaccttac cagtaacaac aggtaatact agaaaacctc
     3241 catgaggtgc gggaacttgg acaccaaaca tatatgtcag tacagcagca attgatgaac
     3301 ctagcatcat agttggcaga acacgcagcg gatctttagc cgcaaatgga attgctcctt
     3361 cggtaatgtg cgttgagccc aagataaagt tgactaaacc agcgttgcga tcattcgtgt
     3421 cgaaatattt tccaaaaaac agtgcggcaa atccagtaat caatgggggc gcgatacaag
     3481 ctgccgaaac ccctgccata aatgtatggt taccttgtgc caacaacgca gtccctgtaa
     3541 cataagccgc tttattgact ggacctccca tatcaaatgc tgacatacag ccaacgataa
     3601 tccctaaaat taatggatta gaattttgga atcctgccaa gaaattcatc attccttggt
     3661 tgactgcttc cattggattt gctaataaag acatgactac accaacaaac aagacaccga
     3721 tcaccggata taagaagatc gccttcagac catccaattg tttaggcaag cctttcatga
     3781 ccttttgcaa tagtaagata aagtagcctg ctaagaaacc tgcaaaaatt ccgcctaaga
     3841 aaccggctcc gccagaattg gctaacagtc cgccaacaaa cccaacaacc aaaccaggac
     3901 gtttagcgat tccttcagcg atataagctg ctaaaatagg aaccatcatt cccattgccg
     3961 catcaccaac accctttaac atagcggcag tcggattgta agtaggatca ttgggatcaa
     4021 aagagtacac accccagatg gcaaacgaaa ttgcaataat taccccacct gccactacaa
     4081 acggcagcat atgagaaaca ccgttcatga gatttttata aatactgtga ccaatgcctg
     4141 ctgcaacccc catctccgct ttctcaccag cagcgtcaac tgttttgttt tctgcagtga
     4201 ttgaaccttt accagccaac gcatcttcga tcaattggtc agcatcttta atccccttgc
     4261 tgacaggcac atcgattaca cgcttacctg cgaaacgttc tgcttgaaca tctttgtcag
     4321 ctgcaatgat tacagcgtca gcgtttttta tttcttctgg tgttaattca ttctctatcc
     4381 ctacttgacc atgggtttca actttgatag ataccccttt gcgcttagct gcctgctcca
     4441 atgcttcttg agccatataa gtgtgggcaa ttcctgttgg acatcctgtt gcggcaacaa
     4501 ttttgtaagt tgtcattttt tactcgctcc ctctttgatt tcgatttgtt tttccaactc
     4561 tgcaacatcc gaaaaatctg ttaatccttt acgaaaagcg gttgaacttc cagctgctaa
     4621 acttttcttc aaattaatat ctacctgttc accttttaaa tatcctgcaa gaaatgttcc
     4681 caacaaagtg tcaccagcac acgctgtatt gactacttta cctttaggag aattcccaga
     4741 ataaacgata tccttattta aaaatattcc tccctctcct cctagagaaa ggagaacatt
     4801 tttcgctcca tcggcaatca agcgttcagc ataatgaata tatccttcac gatcactaag
     4861 ctcgacatta taccaagatt gcaattcctc ctcgtttggt tttaataaga acggctgata
     4921 cggcagacaa tcaattattt ccggatagct tgaatcaata atcagatgga ccttattttc
     4981 aaaacaaata cgactgatat caataagaat ttcgggtgag acccctcgcg gcaaactgcc
     5041 agaaacacac aaataatcgt cagcttgcag cttttgtatt tgatttagaa aagttttgat
     5101 ttgttcctga ggaatctcag gtcctttatt aaccaatttg aattcttcat tggttttatt
     5161 aacttgtgta aaaacgttga ttctagtcat tcccggtacg ctaataaaat cagtgctaat
     5221 atctttttga tgcaaataat cctcaatata attcccggta aaacctgctt taaatcctaa
     5281 agctgtacta tcgataccta attgcttcat gatcaatgaa acattcacac cttttccatt
     5341 agcttgtata tcatcatcgt ttgttcgatt gacttgggat ggtttcattt catctgtttc
     5401 gataaaaagg tcgattgcca agtttaaagt acatgtataa actgacattt acgctcctcc
     5461 tttcttacat attcattatg tatcaagatc gaatgtaaat gttttcataa atgactaaat
     5521 tcattgaaaa cttttacaaa atatattaaa taaacttgaa gtaaaggaaa ctcccgcttt
     5581 acaatagaat tatcctaact aatcggaggc aaaatatgga tacaaaatta agtgaatcgg
     5641 agaaatatct ctgggatttt attcaaagtc acatactcga aatcccctcc tattccattg
     5701 taaaactaag tgaatatgcc aacgtttcaa cagcaaccgt cgttcgtact atgaagaaaa
     5761 aaggatatga ggggtttacg tcttttaaac attcgttaaa agaaaaagaa aatacaaata
     5821 tcaattttgc cgctcttgat aaagttgatg aaggaataaa gactgctatt cttaaaaatg
     5881 agcaagaggt aatccgcacc atcaatatgg tagaaagcgg gaatattgaa gatgctattc
     5941 aacgaatcaa ggctgctaga agaatcatga tttttgcccg aggtttttca gaattgattg
     6001 ctcaggaaat gatggtcaaa tttcaactta ccggtaaata ttgtgagatg catactgatc
     6061 ccaatatcat aaaaaacatt agtagaaaat tatccaaaga tgacatcgtg atctttgttt
     6121 cactaaacgg tgaaacaaag gaattgacta ccgcagctga aaactgttat aaagctgaga
     6181 tcgggacaat tttgcttact gctaatcgtc attcaagttt gatggaacat atcgagattc
     6241 cttttgttgg ctttaaatca gaaggctcct tcttcccaga ttatgaggta cgctccagac
     6301 ttccactttc tatactggct agaatcttac ttgactccta tgcactgcga acatcaggat
     6361 tcacacagca ctaactctat atctgtaaga ctgtacctaa aatatatctg tgtgaattaa
     6421 ctaaggttgt ctagaaaaat atttaatcgc tatttgtatt ctcgtgcttt ctatcattgc
     6481 tccttctata acttcttagt ttcaagatag aatagtgcat tgagactacc aggactactt
     6541 acgcaaatat ataataataa gaatgtgata agcagttcga agttcctgtc taatatttct
     6601 tgttaattat tatcaatttc caacaattct agaattataa tacgcaaaaa aaggagaccg
     6661 tctaaccgtc tccttatagt ttattattca gattacaatc agttatctaa aatgttacag
     6721 aagttttatt agggatattt tcttatcttt ttagatgagg tttctcaatt atttagaaat
     6781 gcttacttaa gttattctcg ttttttgtaa ttacgccaat aatcactata tgttatttat
     6841 ataaaactag tatctaagta tttttatgct actcaacaag agaatcacta tcgtttcctg
     6901 tcttcggtat ttttcgaatg cccaaaatag ttaataaagc acctagcacc gcaaaaccag
     6961 taccgaaaat cagcatctcc catacaccga aatgctgcag cagactgccg cccaagacat
     7021 ttcctatcac cccaccaaga gttgtggcga tgatgatcat cgtttgtcca aatacacgat
     7081 catcatccgc cagaaattga ttgaacaaat aagccgaagc tggaataaac aaagcgaaac
     7141 tgaaggcttg taggagatgc gtcatttcca tcatcaggat cgaactggcc agcagcatca
     7201 tgaatcctct gacagcaaat gaaatactgg caacagacag ccaaaatttg ctggatttct
     7261 ttttgacaag ctgttcaaaa aagatcattc ccggtaattc gcataaagcg gcgatcatca
     7321 atgcgatgcc aacttcttta ctgccgccgt ggatattttc aaagatctgt gccaaaaagc
     7381 tgttgatcat ggtgtggaaa gtaaaaagaa aactgaaacc taaaaataac aaaatcagga
     7441 aaggatattt ttttctcaaa gaaactgagc gatcctgttc ttttgtcata ggaaaaactt
     7501 cttgattttt tacatccggc agcagatgca aaataacgaa aaatatcact gcttcaacta
     7561 taaccaagat tggcaaaatc gcggcagaat aaatcgctaa cagctgtccc agtaaaaaag
     7621 aagttacagc ataagaaaat gaaccaccag ctcttgaaaa gccaaagttg atcctgtagc
     7681 cggtatttga atactcaaat gccagcgcat tgagaaatga ttgcatagaa actactaaaa
     7741 tcccacccaa gatataagta atgatcggca aattcggaat caatacccca gctaagagaa
     7801 tcactcctaa tcctgctatc atcgtcttca gcacggtgat cagtgggatc tttcttttct
     7861 tgatgatcac ttgactgaca aaaggctgaa gcaccgttga aacaatattg accaatgcta
     7921 agatccctcc gattttagct ggatcaatct tgaaataaat caaaaacaat gtcgcaaacc
     7981 catagacaga acaaaacatc atccaataaa gaaaatgcaa acttgtataa taaaaacgaa
     8041 tcatttttcc gcttgtttga tcgcgcacac atctcccacc ttattggttt ttctttagca
     8101 atttactata gcatacttgt aggggtttta ctatttttaa aagtggtgaa gaactatgga
     8161 aattagggta gattacaata tgcgttgatc aattaaaaca tctgttaata aagacggaaa
     8221 aaagatcata gaagcatcgt acttttttag catactagct tggtcatttt tttctggaat
     8281 tgaagaaaat atatatattc taaaaactat ccatcatctc ttagattacg agcttttttt
     8341 gctgcaaagg aagttaaaaa aagcagggat cgagtaatcg attcctgcta tatcttgtac
     8401 caatatattt ctttccaaaa cttaatatac attgtatact tcaatcaata tgattcatta
     8461 aaacaagttt cattattcgt gataaagaaa tcataatttc ggtagtagaa tcattcatta
     8521 tcttttttta caactgctct tttttcttcc gaactttgat ctaaagtaat tttggaatgg
     8581 atatcaagtt cttgaaagac atccatcatt tcagtttgat ctttatcaaa aatcattgaa
     8641 aaagaattat tcttgctttt gtaaaatgaa ataaatgatt tatcgtcacc tagtttttct
     8701 acttggactt gaggatatcg attaaagtta ccgttcaata ctccaaatct aacaacagaa
     8761 tcttttgcta gaccctcctt caaaaaacca ttgatcaaaa tgacagaagc aaaagcaacc
     8821 agtttaatct gatcagtcag atgttccggc cagaaaatca acaggatggc gactgtcagt
     8881 aaaatactca aactcttcct tacaggacta ctctgagcaa acacgataat tttcttttga
     8941 tcgattattt gcaaaataat cagcagtcct aaaaaaactg tgatcataag tgcttctgcc
     9001 atctttcatc atcctttatt ttcatttatc accactcagc gatcgtaccg tcataatttt
     9061 tccattgcgg attggtccaa atcaaacctt cttgtgcaat ttctttgatt ttgtcttcat
     9121 ccatttccaa gcccagacct ggttttgaag gtagatctac gtagccgtct ttatattgga
     9181 aaacttcttt gttcttcaca aaatctaaca gatcaaaacc tttgttatag tggatcccta
     9241 agctttgttc ttggataaag acgtttggcg tacatgagtc tacttgcaga gttgctgcta
     9301 gtgcaacagg accatatggc gcatgaggcg caacagccat gtcgtaagct tccgccatcg
     9361 ctgcgatttt acgggtttcc aagattcccc cacaaagagc cacatctggt tggataatat
     9421 caacagtacc ttgtttaaag aggttcttga attgccaacg agtgtacaag cgttcaccag
     9481 tagctaatgg tacagccaca gaatcagcta cttctttaaa gtgttcttca ttttcaggta
     9541 aaacgacttc ttccaaaaac attggatgga atggctccaa agcttttgct agcacttttg
     9601 ccatcggttt atggacccgt ccatgaaaat caacaccaat atccattgca tcaccaaaac
     9661 gttcgcgaat agacgcaact cgttccacca cttcatcaac tttttggtag gaatcgatat
     9721 aatgcagctc ttctgtcgcg ttcatcttga cggccgtaaa gccccgatca aaccgatctt
     9781 gtgcttgttc tgcaacttca cttggtcgat ctccaccaat ccaagaataa accttgattt
     9841 tatcacgtgc tgcaccgccc aataattcat aaaccggaac attaaacgct tttcctttga
     9901 tatcccacag agccatttct aaacccgaaa cgatggttcc attaatagga ccgcctcgga
     9961 agaaagatcg atgcatttct tggaagaggc gctcgatttc aaacggatta cgtccaatca
    10021 gccggtttcc tatctcataa gctccagcaa caaccgtttc tgttttagtt cctgagatca
    10081 tttctcccca gccttcgatt ccttcgtcag tcgagatctt aacaaaaatc catcttggtt
    10141 ttactttata cactgtgaca tctgaaattt tcaatttaaa ccctcctact attgtgcctt
    10201 caatggttca tccatttcat gattaacttt tttgatacca tcgaagtaat accatgcgcc
    10261 cgcaaaggct aataacaaca ctggaatacc gatgattggt ttgtaagcaa atgcgataaa
    10321 taccaaataa ctttggatca tggaagtcgc agcaaatgat gagattaaca ttgaagaagt
    10381 tcctaagtta ataccaaccg atttggcaat tgctgtcagc attggcgcag tagctgaccc
    10441 acaccatagc aatgaagctg tcacaactaa gccaataatg atatttttta ataaattacc
    10501 acgagtcaag gcaacgacca ttgccacgcg gaaaggaaca accgctaaat ctgcgaatgg
    10561 taacacacgg tttcctggca atactaaagc taatgcaatc gttagtggaa taatgatcaa
    10621 tgctgttgtg ataacatctg gattacctac aacaactgca gcatccaatc cgataaataa
    10681 acgtgtacct ttgaattttt tttgtgacca tttttgtgca gcatctgaaa ttggcatcaa
    10741 tccttccata aataatgaag tcatcttagg aatcaaaacc aaaacagctg acatgctgac
    10801 acctaattgt aaaaccgctt tgatatcata gccggctaaa acaccaatca ccatcccaat
    10861 aactaacccc atgatcatag aatcaccaaa aaatcccaaa tatttttgtg catctttgat
    10921 ggtgaatttt gattttctga ataacgggat acgatccagc agccaattta acggccaagc
    10981 aataaccagt gatgaaagcg cagaaacagt tggtaaagaa acaccaggga ttccgaagaa
    11041 actttctacc atcggttgcg accagtctga aatcttaaaa gtcgcaattg ccatcactac
    11101 tgatgcacct acacctaaga agacattctt agtcacaaaa tacactataa caccaacgat
    11161 tgccatatgg tgatagttcc agatatcaac atccagcgta tgtgtacgtt tcaaaatcag
    11221 caaaacgata ttgaccaata gaatggcaaa aatcaagatt ggaatgattg gcgatgccca
    11281 agtaacagcc gcaatcgaac cccagccaac atccaaagca tctagtttaa caccagttcg
    11341 atcaaccatc gccttggcag ctggtccaac attggtcgtc ataaagtcca atatcaactt
    11401 gatcccggca aaaccgatac ctaatgtcaa acctgattta aaagcacgag aaatctttaa
    11461 cccaaaaatc aaaccaataa tcgtgatgat gactggcaac ataactgttg gtccagcatc
    11521 aataaagaag ttaaagacgt tcataatgat tttcattttt tcagctcctt tgatcagcta
    11581 ttatttcagt aagttcaaaa tctcttccat tgtctgatcg gcaccgatac ctgttaaaat
    11641 cggcatccct ttgattactg gtacaccacc tgtttgacca tcaaattgac cagtcgtcac
    11701 caatagatca tagtcttgaa cttttcctgg aacttccatc aattttgttt gagtcgttga
    11761 aacatcaatg ccatgttgtt gcaaatactc tctaactttt gttgctacta ctgttgaagt
    11821 ggcgatacca tttccgcatg ctactaaaat tcgtttcatt atgatttcct ccttatatta
    11881 aaaaaccgct ttctttaata gttctaataa ttcagtatca ttgcctgctc tgatcatttc
    11941 ttgccgcagt ggctcatctt gtatgattcc aacaattttt tgtaacattt caacttgccc
    12001 atgtggttcc ccaatcgcca tcatgatcac aatttggatt ggtaactgct cattgttatc
    12061 ttccatatta tgaaatagga ccggatcttt aagagtcgca attgctaaag ttgttttatt
    12121 gaccatttca tagtttgcat ggggaattgc gattcctggt ggtgttgatg gtaatccagt
    12181 agggtattct ttttcccgtt cttggattgc ttgaatatat tcctccttca cgtagttttt
    12241 tttgtacatc tcgcttgcta aaaacgctaa gacttcacta tctgtttttg cttgaacatt
    12301 tcgaaaaacg agttcctcat tcaaataaag ttccaaagtc ctcactccta tttaatagcc
    12361 tcaattaatt ctttcatcga acgtgctaag tttttaacat ttagctcttc gatgtcttgc
    12421 ttgttgaaca taccggaacc aagacctaaa tagctagcac cttgttcaaa aaactctttc
    12481 gcattctcct tactgattcc accaaccccc attagtggta acttacctaa aggtgcttga
    12541 atatccttga aaaaacgtgg agtaacaacc gctgcaggaa atacttttac aatatcagct
    12601 ccttctttaa acatacggtc gacttctgaa ggagtcattg cggcaggtac cgccaataat
    12661 cctttcttat tggcgtaaga caacatttct tttgtgaaca tatgtggtcc taataaaaat
    12721 tttgcaccag cctcatagca ggcttgggct tgttctaatg tccttaccgt cccagcgccg
    12781 atataagtcg tgtcaccata acgttgattt agttcatgta tattttcaag agctcctttt
    12841 gtgttcattg tcatctctac ggcaaattga tggtcaaatt cctgcattgc ttgtaaaata
    12901 gcatctgcct gatcaaaagt ataaccacgc atgatcactg tcaatttagg ataatctgct
    12961 agtctcatgt tttcctcctt aagatctttg tcttaatagt tgataaactg tcttttcgtc
    13021 atcagcaatc aataactgct caatcaatga atcatctttg gcgatatcag cgatttgatt
    13081 gatggctcgc agatgcttcg ttaaatcaaa aaatgaaagc ggcaaaatca agctgacatc
    13141 tttaccatta ggaaatcgta tcggcttttt acaaactaaa atgctgacac cgtcactgat
    13201 tacaccttta tcgaccgttg tatggggaat gcaagtcttc attcccaagt aacctgcata
    13261 tcccggttgt ctgatttgat ccaagcaatc agcgataaaa cgcgagtcaa tgatttgatg
    13321 ttccaataac ggtcggcaag ccacctccac tgctcgttcc caagacatct cttcttcaac
    13381 gatttgtaca taactcggcc gcaagtaata agtcaatgaa ggcaatacat tgaagttgtc
    13441 cagcggcgtg cttccatctt ccttgatcaa aaagtactgc agttcttctc gcaacgaaac
    13501 tggattggtg atatttgcat atttcctaat gattgttaac aattcatcca ttgctcgatc
    13561 aaccttagta attcctaaat cactcaaaac tcgatatcgt aaactgattt gttgctgata
    13621 tgtcatgatt ggctcgatca taaactgcag catagaactt tttagtggcg tcgtagtaaa
    13681 gaccagatca taatcagctt caaatttgta gaaatcacgc acagataggg aagctaaaaa
    13741 atggatctct ggaaaaagtt tttccatatt ggctcgtact aactttgaga ccatgacacc
    13801 attagtacaa acaactacag ccctttgtcg ttgcttattc aatttattat gattacttaa
    13861 ttgatagcca aagtaatagg ataaaagatc taattcatca tttggaaagg ccttatttag
    13921 ccaattctca ataggaataa tcaattcttt catcaaatca atcaaaatcg catgattact
    13981 atccttgatc agactatcta ttgaataaac tcctaaactc aagttatagc gtattcgaaa
    14041 acaggctgga cgcagatgac tcagtatcct acgttcaaag tcttctcggt catcgatcaa
    14101 aatcaacgtt tgattttgga aattttcaac catttggctg atcaattttc gcaattcttg
    14161 atccgaatca taattttgag tagttttgct ctcaaaaata ttggaagtta aaaataataa
    14221 cgtgaaccac tccatataac ttttggatag actccaacct gtttcatgta ccaagatcat
    14281 cagcattcgg aattcaggag tatccgtcac atcatcggga aaaaacgcgt gcttatctga
    14341 catcttgatc ccacggctta ctaaaaatct cgctgatgtc tgcaaatagt ctaccgactc
    14401 atcagaataa ctaagatgca acatttgttc catgttatgg atcaaatgga cgatttcttc
    14461 ttcagaaaca tcttgtgcca attttctgcg aatcatttct cggcgaaaaa tcggaaaccg
    14521 ttttactaga tccgataaca gctgtaagat acggtgttcc gaaccaacca attggtaacc
    14581 attcatccga ttatatcgaa ttgtcagact ataatttctg atgaacggtt ccgttcgttt
    14641 gatatcttcc ataacagtcg tcttactaac atcaaccgca tctaccaaat gatccagact
    14701 gatgtaatcg atattagcga tcagagtcat cactatcaat gcttgtcttt cactatcagt
    14761 gagaacgaca agttcctgat catcacctat gtcgtaatca ggctgtgata tcatttggat
    14821 aacctcgatt ggtaccgtaa tatttccaga atgacttctc tgaattaacg gaatgttctt
    14881 tgttttcaat tcttcattaa attgattgat cgcgtaattg atttgacgac gtgtcaaatc
    14941 aagttcactt gttaaagtgg aaagtttcat ttcagggttt ttaataatca gtttgagtat
    15001 ttcacgaatt cgtttattca ttttgactat cctcctgaca aaatcattat atacttgttc
    15061 acatacgttt ccggaaagct ttcatcacgt tttttgtcac acttggttta atgttttgtc
    15121 acactaacag ctatttttaa aatgaacgct gttatatcaa atgtaacatg gtatgacaat
    15181 tattttaggg ctaatgtgaa tattaaatat tcggtagaat attggatgct ttagtaaata
    15241 aacacgggac gttgcttctt caacacatta aagtatctat tccgtaacta aatcaagtca
    15301 gtctaattga aaaagaataa tattaaactg ctaaaaacaa tgcttttggg agcgtcaata
    15361 attttgtgta aatggttctt ccctactgta gattgtttct aattctccat cggttgactc
    15421 agcatttgtt cgagctcgcc ttcggcctgt tggaaacctt tatgactgcg acctaggaat
    15481 ttttggttgt aagtatcaaa agacgagact aagaaacgtt ccatcgactc ttcattttgg
    15541 aactgttcct tgcggtggct gtatttcttg atttgcttat taaaggattc aatcaagttc
    15601 gtagagtaaa tagtcttgcg tatggctaat gggaaatcat agaaagtgag caggtaatcg
    15661 ttcgaaagga tcgattccac catttttgga taactggttt tccatttttc cgcaaaagca
    15721 ccacgtgctt ccaatgccgc ctttttagat gacgcttgat acaccatttt aaaatcatca
    15781 cagacttcct tacgatcatc gacacgcact ttgtgactga tattacggga aacgtgcaca
    15841 cagcaatgtt gaaaacgggc tttggggtag aaccgctgca cggcccctgc catccccttt
    15901 aagccatccg tgatgaaaag gaggacgttt ttcacgccac gttcctggag gtctattaaa
    15961 atctcttccc aaatcgtgat agattcagtt ggtgcaatcg catagctcaa cacttccttt
    16021 gaaccgtccg ggcgaatgcc aactacgata tgaatggctt ccttggcgag ggttttccgc
    16081 tttaaaggga tataggttgc gtccatataa atagcggcat aacggtcatg aagctcacgt
    16141 tccttaaatg aagtgacttc ttccgtaaat gatttagtca tattggacat ggtttggggc
    16201 gtgtagtgat gcccatacat tttctcaatc aagtctgcga tttccgacat ggtaatacct
    16261 tttcggaaga ggtgaatgac ggtctcctct aaggtatcat tcgtccgtct ataagcagga
    16321 acagtctgtt gcttgaactc gccgttgcgg tcgcgcggaa tctggagatg aagttccccg
    16381 tactcggtct tgaccgtacg gtcataggag ccgttacgag aattacccgt gttaaaacca
    16441 atgcgatcgt acttttcgta atcgaggaaa gccgttaact ccgttttgag aagcgtattg
    16501 acagcttttt ccaaatggac acggaataat tcattcaaat cgccttttta ctgataaaac
    16561 gataaaatga tagtggttcg tttgttgcat actcatttaa tagatgttga attaaaatat
    16621 agttcttcga ataattactt tttgatttaa catctgcttc accacttact agaatactag
    16681 caaattcttc attcccctca tcccccatta ctcgagacat aatcagaatt ttatctggtt
    16741 taacctcacc tgttaaatca tcttgttccc ataacgcggg ttcaatttgg cttttcaaga
    16801 ataaagcttc cttttcacaa ctattttcta atttagcata gatagctctc aaaaataaaa
    16861 atagcgtagt tatcctttgc tgtccatcaa tgatttcttg ctcgttatga tcattttcat
    16921 aagccacaat gcttcctaaa aagtaagtac tctcgttatt gtttgctgtg tactccgcta
    16981 aatcatcaaa tagtacttgg ttttgctcgt cagtccaggc ataaggacgt tgatattctg
    17041 gaataacgaa ttttttgatt ttccctgttt caagaaactg cttcacgctt tgtttgttca
    17101 cttcaattgt cgttgacata gtttaaatct ccgagacgac actgtttata aaaataaatc
    17161 tatttccttt attctgacat agctgatatg acagagttca gacaatttag tacgtaacaa
    17221 atttttcagg gctttcttaa aagcaaacaa tacaacttct cctttatttt tgagagaaat
    17281 tgtattgtct aatccttatt tataaatttt atttcgtccg taataacttg tataaatctc
    17341 tagcaactaa tgtatatact agatcaaagt acttatcatc atgcccaaac attttctcaa
    17401 aagctgcatt gttcttctcg tactgctcca tcaccttatc tttaaataca tcagggaaca
    17461 gcgatttttc aaacatctga gcatcgttat tcttagccat gctgacaacc ttcaaatctg
    17521 ggttatcggc aaatgaccga tacaaaatct ctactaacac ccggtctcct tcgtcaaaat
    17581 catttggaaa tcgttcattg attttatgaa tgatttcttc tagtgaatct cgttcatccg
    17641 gcggtttgat tcctgcatca attgactttg gatttactag ctcgccttct ttattcacta
    17701 gatggatttc accggaaaag tcctgacgta acttataata ttccaacttt actttgtcag
    17761 aaatatcaat attttctcgt gtttctttag gtagaaactt cagcaagtat ccagtaaaaa
    17821 ttcgttcctc caatagttcc atatcgaata accgatcaag ttgtgaaata taatcatacc
    17881 acttgccaaa gttacgtaga gtcacccgaa actggtactg attatccttg ttcatttcct
    17941 catagcgatc cataatcggc ttaaacatgc tagaaattcg tccaagcaag cgctcattct
    18001 gttgcttttg ggcttgtttc accaatttca tgacacgttc aatatcacct tgattgtaaa
    18061 cattaaattt tcgtagcttg ctttgagtat cataaatcaa gttaacattg atttcttctc
    18121 tcaaatttgt ttgttcataa aagggttgga aagcagcttc aatatcttca acatcattta
    18181 caaaatcaag aataaaggta tccttcttcc caggcattgt tcgattgagt cgtgataaag
    18241 tctgaacagc tttaacaccc tttaacttct tatcgacaaa catggtatgc agcaacggtt
    18301 catcaaaacc cgtttgatat ttttcagcaa caatcaagat attaaaatta tctgtatgga
    18361 aagcttcctt taattgattc tcccgaacat tctcaccaga ttttgtgaca ttcatcttcg
    18421 tttctgtata ttcagtctct ccatctacta ccgaaccaga aaaagcaacc aacacatcca
    18481 agtcattata acctttgttc gctatatatt ttttaaattc tttcatatag cgaacagcgt
    18541 gtaagcgtga tgcggtaacg accattgctt ttgcctgccc accaatagct gtacgagtaa
    18601 ccgctcggaa ttgctcaacc atcacagccg tcttttgagt aactgcccaa ggatgcaatg
    18661 attggaaacg aacaatcgct cgggttgctt cactctttgg taattctggg ttatcttcaa
    18721 tttcacgggc aattttgtag gaagtactat aggtcatata gttcttcagc acatctaaga
    18781 taaagccttc ctcaatcgcc tggcgcatcg agtaaacatg aaagggttcg aaatgcccat
    18841 ctggttgttt cgtaccaaac atttccaatg tcttttcttt cggtgtagcg gtaaaagcaa
    18901 agaaacttaa attttgatgt ttaccttgtg ctaccaattg ttgaactagt tcatcttcag
    18961 catctggtaa tacatcgtca acttcgcctt ccagttcata atactcttcc aaggatttag
    19021 atttgtccga aagagcggct tttagcttct gggcactgtt cccagtttgt gaagaatgtg
    19081 cctcatccac aatcacagca aaattacgac cttccacttg atctacttcc tgatagatca
    19141 ctgggaactt ttgcaaagtg gtaataatga tcttacgccc atcattaatg gcttctttta
    19201 aatcttgaga ggatttgcga tcgtcaatcg tttctaccaa acctgtctga tgatcaaagt
    19261 tcatgatagt attttgcaac tggcgatcca acactgttcg atcagtcaca ataataattg
    19321 atgaaaaaat tgggtagttc agttgatcat gaatagaggc taaatggtat gccagccatg
    19381 caatactatt agattttcca gaaccagcac tatgctggat caaataattt tctccactac
    19441 catggctttt tacatctgca accaattttc gaaccacatc taactgatga taacgaggaa
    19501 aaatcaacgt tttctttgtg acttctctgt cgccttgctt ttctttttta atctctagat
    19561 tcataaaacg ttggataata tccatcaaat tgtctctagt aagcaccttt tcccacaagt
    19621 aggcagttgg gtagccatct ttattggacg gattgcctgc tccaccaact cttcccggcc
    19681 cattggaacc ttgattgaat ggcaagaagt acgttttttt accgtccaac tttgtcgtca
    19741 tataaacatc tgtatggtcc accgcaaaat agactaagaa gcggcgatta aaacgaaata
    19801 ccaattcttt tggatcacga tctttttgaa actggagttt ggcattttct actgtttgac
    19861 cagtgtactg gtttttcaat tctaaagcaa cgagcggaat accatttaac gacaaaacca
    19921 tatcaatggt tttatggtta ttaggactgt aagcaaactg gcgagtacag gtaaaactat
    19981 ttgcttcata cttttccatc gtttcatcat tcagctctga agcaggtttg aagtaagcta
    20041 ctttaaagcg cacaccgcgg tctttgatcc cattgcgcaa tacatgtaac atgccgtggg
    20101 tttctacctc ttgattaaag cgttgaagga acttgacttc tccatcattg ttatagtttc
    20161 gttggtgacg tgcccactgt tttggttgag tcttctggac aaagcctagc aatttttgac
    20221 aatccaaacc aattatggga tcaaagtttg attcattgaa tgtttctttc ttccatttgc
    20281 catacgtagt cagtgaagtc tcaatatcct gctcaaaacg aatttctttg ttctctacca
    20341 aatgaatcac ctcctaggct tcttttttac cggttacgta ttcgtagatg agagcttttt
    20401 tataactctc aagttccatc aattcagcta tcttattatc aatcatttga tttattat
//
LOCUS       DAZKSU010000047        20400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000047 DAZKSU010000000
VERSION     DAZKSU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.047"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..409
                     /locus_tag="ACG8Z3_001778"
     CDS             134..409
                     /locus_tag="ACG8Z3_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0824086.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="ACG8Z3_001779"
     CDS             415..1182
                     /locus_tag="ACG8Z3_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0824087.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="ACG8Z3_001780"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="ACG8Z3_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0824088.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3439..5211
                     /gene="recQ"
                     /locus_tag="ACG8Z3_001781"
     CDS             3439..5211
                     /gene="recQ"
                     /locus_tag="ACG8Z3_001781"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0824089.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLRKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5325..5627
                     /locus_tag="ACG8Z3_001782"
     CDS             5325..5627
                     /locus_tag="ACG8Z3_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0824090.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5630..6793
                     /locus_tag="ACG8Z3_001783"
     CDS             5630..6793
                     /locus_tag="ACG8Z3_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0824091.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6819..10247
                     /locus_tag="ACG8Z3_001784"
     CDS             6819..10247
                     /locus_tag="ACG8Z3_001784"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0824092.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10268..11392
                     /locus_tag="ACG8Z3_001785"
     CDS             10268..11392
                     /locus_tag="ACG8Z3_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0824093.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11373..11819
                     /locus_tag="ACG8Z3_001786"
     CDS             11373..11819
                     /locus_tag="ACG8Z3_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0824094.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11803..12141
                     /locus_tag="ACG8Z3_001787"
     CDS             11803..12141
                     /locus_tag="ACG8Z3_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0824095.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12260..12820
                     /gene="rsmD"
                     /locus_tag="ACG8Z3_001788"
     CDS             12260..12820
                     /gene="rsmD"
                     /locus_tag="ACG8Z3_001788"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0824096.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12817..13308
                     /gene="coaD"
                     /locus_tag="ACG8Z3_001789"
     CDS             12817..13308
                     /gene="coaD"
                     /locus_tag="ACG8Z3_001789"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0824097.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13298..14341
                     /locus_tag="ACG8Z3_001790"
     CDS             13298..14341
                     /locus_tag="ACG8Z3_001790"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0824098.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14500..15228
                     /locus_tag="ACG8Z3_001791"
     CDS             14500..15228
                     /locus_tag="ACG8Z3_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0824099.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15344..15589
                     /locus_tag="ACG8Z3_001792"
     CDS             15344..15589
                     /locus_tag="ACG8Z3_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0824100.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15586..15804
                     /locus_tag="ACG8Z3_001793"
     CDS             15586..15804
                     /locus_tag="ACG8Z3_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0824101.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15940..16479
                     /locus_tag="ACG8Z3_001794"
     CDS             15940..16479
                     /locus_tag="ACG8Z3_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0824102.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16472..18745
                     /locus_tag="ACG8Z3_001795"
     CDS             16472..18745
                     /locus_tag="ACG8Z3_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0824103.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18783..19829
                     /gene="holA"
                     /locus_tag="ACG8Z3_001796"
     CDS             18783..19829
                     /gene="holA"
                     /locus_tag="ACG8Z3_001796"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0824104.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19918..20172)
                     /gene="rpsT"
                     /locus_tag="ACG8Z3_001797"
     CDS             complement(19918..20172)
                     /gene="rpsT"
                     /locus_tag="ACG8Z3_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0824105.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
BASE COUNT         6837 a         3431 c         4357 g         5775 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaact tatttctaga gagtttttgt ttcataataa
     3361 gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca
     3421 agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggcta tgatgagttt
     3481 agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc
     3541 atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg
     3601 cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat
     3661 caattaggta tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga
     3721 cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt
     3781 atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac
     3841 gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg
     3901 aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact
     3961 gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag
     4021 actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa
     4081 tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg
     4141 agaaaagaag tagaccgtct ctatcatttg cttagaaaat tcgatttttc cgtgggtaga
     4201 tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat
     4261 cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc
     4321 cgttttgtta tccattatca aattcctggc tctctagaag catactatca ggaagctggt
     4381 cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc
     4441 caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa
     4501 tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac
     4561 atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat
     4621 gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt
     4681 atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa
     4741 aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg
     4801 caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc
     4861 aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa
     4921 gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat
     4981 acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc
     5041 ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc
     5101 agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa
     5161 gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga
     5221 atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat
     5281 caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga
     5341 aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa
     5401 atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac
     5461 agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga
     5521 acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg
     5581 tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa
     5641 agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt
     5701 attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa
     5761 tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc
     5821 actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat
     5881 cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc
     5941 gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat
     6001 ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt
     6061 tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat
     6121 tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc
     6181 cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac
     6241 ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta
     6301 tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca
     6361 tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca ggggattagg
     6421 taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat
     6481 cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat
     6541 gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg
     6601 cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag ggattactgg
     6661 cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct
     6721 gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag
     6781 cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta
     6841 acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg
     6901 ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat
     6961 atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt
     7021 ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa
     7081 acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc
     7141 atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga
     7201 ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg
     7261 cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca
     7321 tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag
     7381 caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc
     7441 atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag
     7501 attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga
     7561 atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt
     7621 atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag
     7681 tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg
     7741 taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc
     7801 agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc
     7861 cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt
     7921 ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg
     7981 attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa
     8041 tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc
     8101 tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata
     8161 gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat
     8221 atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt
     8281 atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga
     8341 atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt
     8401 tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc
     8461 ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt
     8521 caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc
     8581 cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt
     8641 tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg
     8701 tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga
     8761 ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag
     8821 ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt
     8881 ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata
     8941 tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga
     9001 cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt
     9061 ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga
     9121 atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc
     9181 cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt
     9241 attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa
     9301 tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc
     9361 gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta
     9421 aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc
     9481 ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga
     9541 ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga
     9601 ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg
     9661 acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg
     9721 aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc
     9781 aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg
     9841 aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg
     9901 gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg
     9961 aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac
    10021 cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga
    10081 aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg
    10141 aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag
    10201 acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg
    10261 gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt
    10321 ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca
    10381 aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac
    10441 ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt
    10501 aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt
    10561 tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc
    10621 tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat
    10681 ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga
    10741 ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt
    10801 tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa
    10861 actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc
    10921 tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct
    10981 tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca
    11041 gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc
    11101 tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga
    11161 tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca
    11221 tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga
    11281 ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt
    11341 agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc
    11401 tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga
    11461 tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga
    11521 aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg
    11581 cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg
    11641 atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa
    11701 ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt
    11761 tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt
    11821 gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta
    11881 aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg
    11941 ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt
    12001 gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata
    12061 ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc
    12121 cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg
    12181 gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc
    12241 atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa
    12301 aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa
    12361 tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg
    12421 tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta
    12481 ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac
    12541 cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt
    12601 cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa
    12661 aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac
    12721 agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat
    12781 aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga
    12841 agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc
    12901 gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa
    12961 gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat
    13021 caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga
    13081 ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat
    13141 ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt
    13201 tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca
    13261 gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa
    13321 aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc
    13381 gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga
    13441 taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac
    13501 aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga
    13561 attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc
    13621 ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga
    13681 attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc
    13741 agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat
    13801 ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg
    13861 tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat
    13921 aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc
    13981 tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt
    14041 cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga
    14101 aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg
    14161 cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc
    14221 tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat
    14281 gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg
    14341 aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc
    14401 aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag
    14461 ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat
    14521 ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta
    14581 gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt
    14641 ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa
    14701 atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa
    14761 gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt
    14821 atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta
    14881 ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc
    14941 ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg
    15001 gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact
    15061 ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga
    15121 tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc
    15181 ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa
    15241 tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga
    15301 gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga
    15361 aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat
    15421 tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga
    15481 tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga
    15541 gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg
    15601 aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca
    15661 aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta
    15721 gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt
    15781 cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg
    15841 atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt
    15901 actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa
    15961 gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg
    16021 gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta
    16081 gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca
    16141 gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg
    16201 cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg
    16261 gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg
    16321 caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa
    16381 gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt
    16441 tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg
    16501 aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga
    16561 caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg
    16621 acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct
    16681 acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga
    16741 tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca
    16801 aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt
    16861 ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg
    16921 gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt
    16981 ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag
    17041 aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata
    17101 tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt
    17161 tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt
    17221 agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc
    17281 gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg
    17341 tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac
    17401 catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt
    17461 tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga
    17521 tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt
    17581 gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc
    17641 accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg
    17701 gctgccggaa actttacttt tgctgataga agagggatta atcttatttg agcgaataat
    17761 cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat
    17821 gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt
    17881 gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc
    17941 aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg
    18001 aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc
    18061 gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg
    18121 gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt
    18181 acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg
    18241 aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga
    18301 aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga
    18361 aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg
    18421 ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc
    18481 taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac
    18541 agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg
    18601 ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg
    18661 gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg
    18721 gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga taatttttga
    18781 aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg
    18841 catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa
    18901 ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg
    18961 aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc
    19021 aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa
    19081 tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat
    19141 tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga
    19201 aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc
    19261 tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct
    19321 tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt
    19381 atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt
    19441 tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt
    19501 tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt
    19561 tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc
    19621 aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg
    19681 aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg
    19741 attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc
    19801 gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag
    19861 gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta
    19921 tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag
    19981 accttttgat tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt
    20041 gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt
    20101 ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc
    20161 aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat
    20221 tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt
    20281 tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa
    20341 aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt
//
LOCUS       DAZKSU010000048        19480 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000048 DAZKSU010000000
VERSION     DAZKSU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.048"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG8Z3_001798"
     CDS             133..3660
                     /locus_tag="ACG8Z3_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0824106.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG8Z3_001799"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG8Z3_001799"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0824107.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG8Z3_001800"
     CDS             7448..7789
                     /locus_tag="ACG8Z3_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0824108.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8Z3_001801"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG8Z3_001801"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0824109.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8Z3_001802"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG8Z3_001802"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0824110.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8Z3_001803"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG8Z3_001803"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0824111.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG8Z3_001804"
     CDS             13000..13737
                     /locus_tag="ACG8Z3_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824112.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG8Z3_001805"
     CDS             13751..14566
                     /locus_tag="ACG8Z3_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0824113.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG8Z3_001806"
     CDS             14579..15226
                     /locus_tag="ACG8Z3_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0824114.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG8Z3_001807"
     CDS             15242..15898
                     /locus_tag="ACG8Z3_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0824115.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG8Z3_001808"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG8Z3_001808"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0824116.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG8Z3_001809"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG8Z3_001809"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0824117.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG8Z3_001810"
     CDS             18240..18758
                     /locus_tag="ACG8Z3_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0824118.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8Z3_001811"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG8Z3_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0824119.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6551 a         3344 c         4182 g         5403 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag
//
LOCUS       DAZKSU010000049        19304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000049 DAZKSU010000000
VERSION     DAZKSU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.049"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1628
                     /locus_tag="ACG8Z3_001812"
     CDS             237..1628
                     /locus_tag="ACG8Z3_001812"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0824120.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1687..2490
                     /locus_tag="ACG8Z3_001813"
     CDS             1687..2490
                     /locus_tag="ACG8Z3_001813"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0824121.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2523..3377
                     /locus_tag="ACG8Z3_001814"
     CDS             2523..3377
                     /locus_tag="ACG8Z3_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HFQ0824122.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3374..4075
                     /locus_tag="ACG8Z3_001815"
     CDS             3374..4075
                     /locus_tag="ACG8Z3_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFQ0824123.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4083..5150
                     /locus_tag="ACG8Z3_001816"
     CDS             4083..5150
                     /locus_tag="ACG8Z3_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0824124.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5143..6108
                     /locus_tag="ACG8Z3_001817"
     CDS             5143..6108
                     /locus_tag="ACG8Z3_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0824125.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6153..7127
                     /locus_tag="ACG8Z3_001818"
     CDS             6153..7127
                     /locus_tag="ACG8Z3_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0824126.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7150..8685
                     /locus_tag="ACG8Z3_001819"
     CDS             7150..8685
                     /locus_tag="ACG8Z3_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFQ0824127.1"
                     /translation="MKKSRLEYSLINSSISMFIYILRIVIQFVGRSFFIYFLGAKYLG
                     LNGLFTNILSFLSLAELGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIG
                     IVGVLIIPLLPLFIKNSTDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAI
                     NDFAFLFFMNIVQVIALYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVV
                     SLDKQTKDEVKKNVIGNMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQ
                     NLCKQITNSITASVGNFAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISL
                     WVGAKYMLPMSTTILIVLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSL
                     LLLIVFDMGINGVLIGTIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELII
                     SAIVVFLLNEYLANGGSTFLQFITKTLITIVTTSILYLLLYFRREEFTFIKKILFRYI
                     LKK"
     gene            8742..9857
                     /locus_tag="ACG8Z3_001820"
     CDS             8742..9857
                     /locus_tag="ACG8Z3_001820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0824128.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            9854..11269
                     /locus_tag="ACG8Z3_001821"
     CDS             9854..11269
                     /locus_tag="ACG8Z3_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0824129.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            11302..12249
                     /locus_tag="ACG8Z3_001822"
     CDS             11302..12249
                     /locus_tag="ACG8Z3_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0824130.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(12289..13449)
                     /locus_tag="ACG8Z3_001823"
     CDS             complement(12289..13449)
                     /locus_tag="ACG8Z3_001823"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0824131.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            13614..14789
                     /gene="mltG"
                     /locus_tag="ACG8Z3_001824"
     CDS             13614..14789
                     /gene="mltG"
                     /locus_tag="ACG8Z3_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0824132.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            14925..15404
                     /gene="greA"
                     /locus_tag="ACG8Z3_001825"
     CDS             14925..15404
                     /gene="greA"
                     /locus_tag="ACG8Z3_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0824133.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            15572..16303
                     /gene="liaF"
                     /locus_tag="ACG8Z3_001826"
     CDS             15572..16303
                     /gene="liaF"
                     /locus_tag="ACG8Z3_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0824134.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDIEVIRI"
     gene            16300..17367
                     /gene="liaS"
                     /locus_tag="ACG8Z3_001827"
     CDS             16300..17367
                     /gene="liaS"
                     /locus_tag="ACG8Z3_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0824135.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            17374..18006
                     /locus_tag="ACG8Z3_001828"
     CDS             17374..18006
                     /locus_tag="ACG8Z3_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0824136.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            18146..18811
                     /locus_tag="ACG8Z3_001829"
     CDS             18146..18811
                     /locus_tag="ACG8Z3_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0824137.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            18839..19132
                     /locus_tag="ACG8Z3_001830"
     CDS             18839..19132
                     /locus_tag="ACG8Z3_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0824138.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(19200..>19304)
                     /locus_tag="ACG8Z3_001831"
     CDS             complement(19200..>19304)
                     /locus_tag="ACG8Z3_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HFQ0824139.1"
                     /translation="DFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         6741 a         2703 c         3713 g         6147 t
ORIGIN      
        1 atatgaatat tgtaacttgt gaataaggaa ttagcaggag aatactagtt cagcataaag
       61 gaagtgatgg gacttctgag acaactttct gttatgcaga aatacacata taaacagaca
      121 ataaacgctt ttccccgttt cttttaattt cctttacaat ttggttattc actaggtttt
      181 taaactttgc tatggtaaga tgatagaggc attattttat ggttaggagt gtatatatga
      241 ataaaaatgg ggagtggaat gcggcacgca gaatattcat tattcttatg gatgtacttg
      301 tattaaatgt atcgatctat cttagttttt tgctgaaatt ccagggagaa gtaccagtga
      361 gaaacttaga aacctttcag cattcagcaa tttttatttc catcatcttt attgcgttga
      421 atattttgtt aggagcttat gtattctata accgaatgat cagtgatatt gtcttcatta
      481 ctgttattgg acaagtacta atagcattgg ggatcatggt agttactttt gctggccgat
      541 ggtttgcatt tccgcgtggc gtcattttga tctcgtttat cctaggtacg atattactta
      601 gtgtttaccg gatagtcgtt tacaaactgt acttaagagt cagtcgtgat aagaaagtcc
      661 ttatcgtggg tttggagaaa gatgtcgcac cagctatcca aaattttcaa tctaagaaaa
      721 acaataaaca taaggttgaa gcagtggcaa tcgataatta ttacgaaaat acaaaaaaat
      781 tgatcgatga tatggatatc gtctatttgg cttctaaaat cccagaagat gaaaaaatca
      841 acatttatca attagtgact aaaaaagaga agaaattatt tttaaatacg acttttgaga
      901 atctgacaat gataaatccg aatattatga attttgaaga tgaaagcatc attgaagctt
      961 ctgggttcag aatccaacca gaatacgaac tattcaagcg tctctttgat atcatcgttt
     1021 cgcttatttt actgatcctc gcctcacctt ttatgctaat cacagcgatt ttggtaaaaa
     1081 caacttcacc tggaccaatc atctataaac aagtacgtat tacgaaaaat caaaaggaat
     1141 tttcaatcta taaattccgt tccatgagtg cgacagctga agcaaaatca ggaccagtct
     1201 tggctaaaag taacgatgca cgtgtgacac cagtaggtaa atttatccgt gctgtacgtt
     1261 ttgacgaatt acctcaaatc tttaatgtat tgaaaggcga tatgtcgatt gtcggtccaa
     1321 ggccagaaag accatttttc gttgatcagt tcaatgaaga aaatccttat tattacttaa
     1381 gacacaatgt acgtgcggga attaccggat atgcacaagt atacggaaaa tatgtatcag
     1441 attataacag taaactacgt tttgatttgt tgtatatcaa aaaatattca ttactattag
     1501 atttgaagat tttacttcaa acaatcaaaa tcttatttga taagatgtca tctcaaggac
     1561 tagatgaaga tgaaaatatt agttcattag cagaaatcga cttttcacaa gagaaaattt
     1621 atcgctagat tggaaaaatt ttgagcaaag aggtaggaga gaacattgga aaataaaaat
     1681 gtaagtatgc tgctttcttt atatgataaa gaaaagccag agtatttaaa ggaagcatta
     1741 actagtattt ttgagcaaac agtaatgcca aaagaaattg tattagttta tgacggccct
     1801 ataaatgaaa aacttcaagt agttgtcact tatttccaac aaaaagatcc tcacttgttt
     1861 acagttgtaa aattagaaaa aaatcaagga ttggggattg ctttagcaat tggattagaa
     1921 catacgaaaa atcgcttagt agccagaatg gatacagatg atataatgga accccaacga
     1981 ttagaaaaac aattagagat cttccaaaaa gatccagaaa tagcgattgt tgggagtaat
     2041 attgaggaat ttactggcac gcttagcaat gttatcggaa aaagaattgt acctgaatat
     2101 aacaatgcaa ttcgtgtatt ttctaaaaga agaaatccct ttaatcatat gaccgttatg
     2161 tttgataaaa aagctatttt ggaagtggga aattaccaac cattaagtgg gtttgaagat
     2221 tattatcttt gggcaagatt attaaaggca ggttataaag gatataatat tcaagaaaca
     2281 ttagtctatg cgcgaactgg caaagatatg tatgcaagaa gaggcggaat gaaatatctt
     2341 ggttctggtc ttatgggtcg ttatcgtatc tggaaagacg gtttagggtc agtaaaagat
     2401 tttataattg tttcttgcgg acatattttt attagcttac taccaaatca attaagagga
     2461 aaattttatc aaacgcaatt aagaaaatag gaggtttttc tttgtatagc actgatccaa
     2521 aaatgaaaaa aatacaacag attacgttga aaatgtctca agagattgtt gatttttgtc
     2581 taaaaaatca acttctttgt tatttctgcg gtggtggtgc catcggtact gtgagagaac
     2641 aagggtttat tccatgggat gatgatttag atttctttat gcctagagag gattatgaaa
     2701 aattttatca actatgggtt aattcagaaa aaagtaaaaa atatcccata cagaaggctt
     2761 ctaaaaatta taatgatcat aattctttta caactatccg cgatgcagat actacattta
     2821 ttaaaactta tcaaagagat ttagatatag tccatggaat tactattgat attttcccgt
     2881 tagatgttgc ccctgtttcg aaatttagcc gtaaaattca aaaactatgg gctttagtat
     2941 atgctttatt ttgttctcaa gtcgtccctg aaaaacatgg tggaataatg gctacaggta
     3001 gtaagatact cttatctatt ttccgctcac ctcgtacgag atataagata tggtcttttg
     3061 ctgaaaaaca aatgacaaaa tataataatg aaccaacggg ctatgtgact gaactttgtg
     3121 tcggacctaa atacatggga aatgtttatc ctgcaaaaga ctttgaatct gctgtgtggg
     3181 tgccattcga agatacagag atgccagtac cagttggtta tgataattat ttaagtcaag
     3241 tttttggtga ctatatgcag cttccgcctg aaaaagatca ggtttctcat catgaagcag
     3301 tttatattga tccagaacat ccatacacaa tgtacaaagg aaaatattat ttaactaaag
     3361 gagcagaaaa aaaatgatta cagctattat tattgctggt ggtgtgggta aacgaatggg
     3421 acaagatatc ccaaaacagt tcattacaat tgaaggaaaa cctattatta tctatacttt
     3481 agaatcattt caacaccatc ctcaagttga ccggatttta gtcgtttgca aatctggttg
     3541 ggaagaaacg atgtgggcgt atatcaaaga attcaatatt agcaaagtca aatgggtcat
     3601 tacaggtgga tcaaagggac aagagtcgat caataatggt gttcagtttt tgaaagacta
     3661 ttctgaagat gatgatacga tcatcatcca tgatggaatc cgtccactag tagacgaact
     3721 ggtattgtca gatgtaatcg ttaaatgtaa agaatttggt aacggtgtaa cctctctacc
     3781 ttacaacgaa caaattttta tcaagaaaac agaagaaacg acggaacagt atgtcgatcg
     3841 taacacattg aggcgtgtct caactccaca agcttatcaa tacggaaaac tacgggcagc
     3901 atatgaccgg gcagtgaaag aagacatcgg tatgactgat tcttcttata cgaataccat
     3961 gatggtagat ttgggagaaa ctttatattt tgctgctggt tctgataaaa atatcaaatt
     4021 gacaacaaca gatgatctag aattgttcaa agcatattta aaaatgaaag actaggtgaa
     4081 agatgatgac gttgattaaa caaccaatgt accaagaaga tttggcaaaa gtaatcaatc
     4141 atacaccaaa aataaaacag ctacatggaa aatccgttct gatgattggt gcttctggaa
     4201 tgatcggctc ttttttgatt gatacgttga tggctgctaa tgaacaacta ggcatcaaaa
     4261 tcaaagttta tgcaatgggc agaaaccgtc agaagcttga aaaaaggttt gccagttatc
     4321 ttgaattaga tactttcgaa attgttgaag gtgacgtcac agaaccctta ccaaaagaaa
     4381 ttcaagctga ctatttgata cacggtgcaa gtaacacaca tcctaaggct tacgcaactg
     4441 atccaatcgg tacgatcatg actaatcttg caggtacaga gcaagtattg aaacatgcag
     4501 tagcaaccca accggttaga gtattatttt tatcaacagt agaaatctat ggcgaaaatc
     4561 gaggcgatat agaaaaattc acagaagatt actgtggata catcgattgt aatacactga
     4621 gagcaggcta ccctgaagga aaaagagtca gtgaatcatt atgccaagcc tacatcgcaa
     4681 aacacgacct cgacatcgtg attccaaggc tttgccgtac gtttggtccg acgatgcttt
     4741 tatctgatac gaaagcatcc tcacaattta ttttaaatgc agtcgaacaa aaagatattg
     4801 ttttaaaaag tgaaggaaac caatattttt cgtatgtata tgttggagat gctgtttctg
     4861 ctattcttca tttactgctt gaaggagaaa aaggagaggc gtacaacgtt tcttcagaaa
     4921 actttgattt acgactaaaa gaattagccc aacagctagc tgatatcagc ggcaaacaag
     4981 taatttttga attgccggat gagattgaac aaaaaggttt ctcaaaagct agcacagcag
     5041 tacttgaaaa taaaaaaatt aaagagacag gttggaaacc aatttttcct ttgaacgaat
     5101 cattggttca tacagtagaa ttagttaaag gtgagaaata taatgtgtga gattagtatt
     5161 attgttcctg tgtataaagt tgaaaaatat ttaagaaaat gtgtagattc gattttagcc
     5221 caaacattta ctgattttga agttatatta gtagatgatg gatctcctga caatagcggg
     5281 aaaatctgtg atgagtatgc tgaaaaagat aatcgagtac gtgtgatcca taaagaaaac
     5341 ggcggattaa gcagtgctag aaatgcgggg attgatgttg cgagaggcaa atacttaggc
     5401 tttgttgaca gtgatgatta tattgatgaa gatatgtatg aaattttgta tgagaatttg
     5461 aaaattcatg atgccgacat tagctctgtt gaattgattc ctttttatgg tgatagatat
     5521 aagaaagcta ataaagaaaa gaaagtaatt atattaaata aaaaggaagc aataaaatct
     5581 gttttagaag gaacgcaatt ttatgcatac gcatggaata aactttatcg taaggaactt
     5641 tttaaagata atcgttactt ggatggaaaa acatttgagg atgcatatat aataatagat
     5701 ctattatttc aaacagaaaa aattgttgtt tccaatgaag agaaatattt ctatcttcaa
     5761 agaaatgata gtattatggg aaaaagtttt tctatgaata atttcgatgt tatagaagca
     5821 tggcaatata acaaagagaa gattttagat gcttttccag atttgcatga ttcctattat
     5881 cgccgactat gttgggcata tttttacgtg ttggataaaa tggttttatc aagtagttat
     5941 caacaaattc cacgtaaaaa aacagtaatt gaatttttaa agaagaacag aaattttata
     6001 ttaacttata agggattcac taaatcgcgc aaaatagcta ttattgctct gagctttaga
     6061 gtagattggt ataggaaatt ggttgaacaa aaaaataaaa aaaattagaa tgtttaaatt
     6121 agtaaataaa agaaaaggaa ttgaaaacta aaatgtgcag aataagtata atagttccag
     6181 tttataatgt tgaaaaatat ttaaaaaagt gtgtcgattc aattctaggc caaacattta
     6241 ccgattttga attattatta attgatgatg gttctacaga taattcagga agtatatgcg
     6301 atgaacttgc gaaaacagac aaccgaataa aagtgataca taaggaaaat gggggtctaa
     6361 gtgatgccag aaatattggt attgaggtag caaagggaga ttttattggg tttatagata
     6421 gtgatgatta cattgatgaa gatatgtatg catttttata taacaatata ttgaaatatg
     6481 atgcagagtt atcaatgtgt ggtatttatg acgtccataa gaataaagaa attaaaaagt
     6541 taactccttt ttctcaattg gttactaaat ctgaagcaat tgaattagta ttagatggta
     6601 agctagttgt tgctaatgca gtaagcaagc tatataaaaa agaattattt gagaatgtga
     6661 gatatccaaa aggaaaaatt gctgaagacg cagcagtaat actgaaaatt atcaatcagt
     6721 gtaataaaat ccatgttgat acttctcaaa aatattatta ttatcatcgt ggagatagta
     6781 ttactagtaa aaaatttaca aaaaaagatt ttgatataat tgatgtatgg aatgagaatg
     6841 aaaaatggat tattaaaaat tatcctcaaa tgtacgataa agtacatact agagtttgtt
     6901 gggcttactt tgttgtgtta gataaattag ttttaactga agaaaaagga ctgtatcaag
     6961 aaaaaaaaca agtaagagag tatttaattg ataatttctt ttttattata aaaaataaac
     7021 gattaacttt tcaaagaaaa atatctatgt tattgcttac ttttggaatt gctgcgtata
     7081 aaataccggt taaatatcag tataagaaag taaaggctgt taattaatgc tttagttagg
     7141 atgaattaga tgaaaaaaag caggttagag tattctttaa taaattcttc tatatcaatg
     7201 tttatatata ttttaaggat agtaatacaa tttgtaggta gaagtttttt tatttatttc
     7261 ttaggagcga aatatttagg attaaatggt ttatttacaa atatacttag tttcttgtcg
     7321 ttggctgaac taggcatagg ctcttctatt atttattccc tatacaaacc attagcccaa
     7381 aaggatgaaa gtaaagtaaa ggctttaatg aatttgtata agaagacata tgaatttatt
     7441 ggtattttta tcggcatagt aggagtattg attattccgc tgttacctct atttattaaa
     7501 aatagcacag acataccaca tatgtatcga tattatgtat tatttttatt aaattcagtt
     7561 cttagttatt ttttcacgta taaaagatca cttgcaatag cagatcaaaa aaattatctg
     7621 gttgcgatta atgattttgc ctttttattc tttatgaaca ttgtacaagt aattgctttg
     7681 tacttttatt ctaattttac tctatttcta atagtgcaga ttttgtttac tctattttct
     7741 aatataaata tttccagaac agttgacaag agatattcat atttaaaaga gaaagatgta
     7801 gtttctctag ataagcaaac aaaagatgaa gtaaagaaaa atgtcatagg taatatgtcc
     7861 tcaaaaatag gcggggttat tgtaatgggg acagataata tattaatttc tacatttgtt
     7921 aatttaacag ctgtaggaat ttattctaac tattcattga ttgtcacaag tgtgcaaaat
     7981 ttatgtaagc aaattactaa ttctatcacg gcaagtgtag gaaattttgc tgtatcaaat
     8041 aaaaaaaagg attcatttga gctatttaaa aaacattttt ttatcaacca ctcactaatc
     8101 tttttttctt ctatattgtt attgacttta atgaatccct tcattagctt gtgggtagga
     8161 gccaaataca tgctacctat gtcaacgact attttaattg ttttaaatta tagtgtacag
     8221 gtttatagaa atacatcctt cgtttttata gagtcatttg gtttatattg gtctcaacgg
     8281 aaaaagccta taattgaagc aggtgtaaac ttgttattat ctttgctttt gttaatagtt
     8341 tttgatatgg gaatcaatgg agtacttata gggactatcg gatcgtctct aggatttgtg
     8401 atttggtatg aagcatttat catatataaa acggtatttg agaaacgttt tattcatttc
     8461 tttttaactt tctggcgaag ttttatagaa ttaataattt cggcaatagt cgtttttcta
     8521 ttaaatgaat atttagcaaa tggaggcagt acgttccttc aatttataac taaaacctta
     8581 ataactatag taacaacatc gatattatat ttgttattat attttagaag agaagaattt
     8641 acatttatca aaaaaattct ttttaggtat attttaaaaa aatagctttc tataaaggag
     8701 tgttttattt gaaaatagct atactatctc cagctttctc aatgggagga gctagtatcg
     8761 tggcctcaaa aattgcaaaa ggattaatag atagtggcca tgaagttttt tttgtttctt
     8821 acttagaaag tgaaaaacca caagtagaat ctaagtatta tgatttatac gaaaaaaaaa
     8881 tttttttatt taaaaatttt tctcgatttc ataagatatt agagtataga ttgtttggag
     8941 gctttgttcc tagtagatat ataaaaaaag aaaaagaaca actaaaaaaa gttctaaaga
     9001 aagaaaaacc tgatgtcatc atattcaata cttttatacc tagtgtacta ttctccgaat
     9061 tcctaaagta tgaatttcca acagtgaaat taattacttg gatgcactct gatcctgatt
     9121 acagcttaaa tcatattgct aaattttata aaaaagaata tagaaaaagt tttgaacgcg
     9181 tggattccat tgtttgtctt tcgcaagata caaaagaaaa actttcatta tacaataacc
     9241 atattaaagt tatttataac ccattgacat taagcggctc tagtttaata agtaaccttg
     9301 ataaaaaaat tatttcattt actgccagat acgatataaa tattaaaggg tttgattatt
     9361 tatgtgaagt agctaaaaac ttacctgttg gatgggctat aaggattgct ggagatggta
     9421 cagaagaaga acgtaaagag ctgaaaagat taattgagaa aaatggtgtg ttagataaaa
     9481 ttatttgcgc aggtgcatta tttggagatg aattaatcaa tcattatata gaaagttcta
     9541 ttttcatatc tacttctaga agtgagggac taccattagt gttgatagaa gcaatgactt
     9601 atggattacc tgtaataagt tttgatcatg gtggtgctaa ggaaatatta caagatggaa
     9661 attatggaat tttaattaag aattttgacg tacaagatat gaataatgag atagaaaaat
     9721 tgtctaataa tcgtttactt atggaaaaat atcaaaaatt ctctttagaa agagctaagg
     9781 attttaatga aaaaaaaata attggtgaat ggcaaacatt gttaaatgaa ttaggaaatg
     9841 aggatgttag aaattgagga cttcgattaa aaattttaat ttatggaaag aatggtatct
     9901 atatatattt tttataggga tgattattcg tgcaataaat ttttatagtt tattaccgtc
     9961 cacttttgat agtatttttt ttaaacttat aggaatttca ggagttttgc tattagggat
    10021 agatttcata attaaattta ataaaagaaa agtaacttat aacattttat tgatagcata
    10081 tatggtgatt ttagtaataa cgagttttgt tcataaagaa tatggtctag gagaaaactt
    10141 aaagactata atgtgggcaa tcttacagta ttttttgatt tatcagtttg cttttgagaa
    10201 tagcgataat aaacgatttt ttaaaaggat aagttgggga tttatttata gttggtttgt
    10261 tttagtatca atgtcaatgg ttcttttctt tggtaaattc ggctatgaga aatattataa
    10321 tgctagacat aggatcagaa ttggtttttt agaatcaaga ctgtttggga cgttttctga
    10381 tattaacaat gctgctatag catctttagt tgttattgtc ctaatcttat tttatcttta
    10441 taataaaaaa gtgacaggat taaataaaat cattttttta attaatcttc cattacaatt
    10501 tatttatata gtattaagtg ggtcacgatc tacctatgtg gtaagtataa taatagttgc
    10561 catatcatct tttattgttg tgttaaaacg aaatggaaca aaaaaagatt taaaaaacta
    10621 tatccttgct atagtatctt ctattggttc tgttctgatt ttattcgctt tgtttaatgc
    10681 aactaagata ctttttcaag aattattacc tcatttaaaa gaaataaatt tttttaattt
    10741 tacagagcgt cataaagaat taatgaataa tagtcaacat attaataata ctcaaacacc
    10801 tgataatact cttgttagga aagacgtagc tgaaaataca gatatttcta atgctagaat
    10861 tactatatgg aaaagtgcat gggaaatatt taaaacaagt tggcttatcg gagtctctcc
    10921 gaaaaatgta gtatcttatg cacagtcaat tattccagat ggatatattg cgaaagtagg
    10981 aattgccatt cataatgcat acttgaatgt attaacatcg acaggtattc taggaataat
    11041 tcctttcatg gcttttttaa taaagagttt tgtgctgaat gtttatcgaa tcatctataa
    11101 taaatgcatt ggtaaagatt atcattttgt ttactgttta gttattttat cttatgcact
    11161 atacgcagct cttaacaatg aattagtcta tgaaaacact gtaggaacgt ttgttttttg
    11221 gctattttta ggaagagtta atagcaaaaa taattctagc tatttttagt aataaatttt
    11281 atcttgaaaa gaggagaaaa aatgttgaat tacttgatta ccggtggcgc cggcttcatt
    11341 ggctcgacgc tcgcgaatta tctaggaaaa gaaaacaatg tagttgtgat cgatgattta
    11401 tcgatgggga aaaaagagaa tctggatgaa tcgaagcaca tcacgtttat tgaaggggat
    11461 gtcgctgaca aaaatttgat ggaaaaggtc atgaaagaat accatttcga ttatattttc
    11521 catctagctg ctgtagctag tgtggcagat tctgtagaac gtcctctaga aactcatcgt
    11581 gtaaattttg acagtgcgct tttattgctt gaattggtac gaaaatatca atctggttta
    11641 aaacgattgg tatttagttc atcagctgca gtttatggcg atgaaccaac gttgccaaaa
    11701 aaagaagaat cggtcatccg tcctttaaca ccatatgcca tcgataagtt tgcagcagaa
    11761 caatatgttt tggattactg tcatttatat gatgttccga ctagtgctgt ccgatttttt
    11821 aacgtttatg gtccaaatca aaatcctaac tctccatata gcggagttat ttcgattcta
    11881 gtggaccgat acaaaaaaca attggcagga gaaaaaacag aatttacttt atttggtgat
    11941 ggtagccaat ctagagactt cgtttatata gaagatgtta tacaagcact tttacttgtt
    12001 gctaaggaag aaaaagcttt aggacagcaa ttcaatgtcg gtacaggaaa aagcactacg
    12061 ctacttgaat tgattcattc aattgatcaa atcttaggaa cagagctagc gcttaaatat
    12121 gaagcggagc gttcagggga tattcgtgat tctttagcgg atatttcaaa gatccgttct
    12181 ttaggttatc aacccaaatt tgatatattg aacggaatgg aacgttattt aaagacagaa
    12241 atcgattgaa acaaaaaacc agcatcctgc tggttttttt gtctcgactt agctttttaa
    12301 attaacacct tccatcagtt cgtaaatctg atttcttaaa tcagtggccg gcaatttgat
    12361 gtttttatca gattggatat tactttccat gacgattgca tcttttgtgt cttttttcat
    12421 cgccgctact gcatggaatc gattgatatt tccctgagca aggatataat catttccact
    12481 gtaaacaccg ccgctgtaag gatgtttatc accattattt tgccacaatg agaaaagtag
    12541 ttcttttggt ataatatcgc cagacagaac actggtaaag aatttgttca agtcataaac
    12601 agacatactt atatttcctg tgcctaattc atttcggatg tctgtttcag gctcgaccaa
    12661 tgcttttgaa taatcgtcag ttgcagacat ttggtaagat acagcatgct gaggggaatt
    12721 ttttacattt tcataaaaat ctgtatgttc tagatggagt ggccgaatga tttctttttt
    12781 taaaagagtt tcataaggct gattcattag tttgatcaat acccctgtaa gcaatgtata
    12841 atttaatggt tcataatgat acgtatcata accagtaaat ttcaaatgat gaagggcaaa
    12901 ctgaatgatc tcactatctg acataggttt ttttggagaa gcggttcgat gaagaccaga
    12961 gcgcatgtaa agcatatttt tgattgagat ttgctgactt ccaggaatct gaggataaaa
    13021 ttcagatagc tttgtgtcaa aagttatctt gccagattga atttgtttta ggataagtaa
    13081 agcagtcatc gctttttgga tcgaaccgat ttgatagtaa agagaaggag aattcgattc
    13141 ttttatgcgt agatttgcgt acccatatcc tttttctaat atcactcgat tatttttgcg
    13201 tacataaatc gaaccgatat aatctgattt ttgcagtaat tgatcaattt tgctggcaag
    13261 catctgctca ttagatgtta aattggaagc aagttgtgcg ggttccgttt tataaatttg
    13321 tggttgaggt tgttttgtta attgccatcc tgtaatgatg cctgttgaaa agaagctgac
    13381 agccagcaac aaaactgcga gaaaagaaag agagatctgc tgtttttttt gatgttttcc
    13441 ttttgccata ttaaatcctt cctatccatt tttaactatt tttcattgta atcctcaaaa
    13501 agagcatttg ccacttacaa ttttgacata atatttggta aaataattca cggcagtaga
    13561 aatttcatgt ataataaaca gagttacgaa gatgatagta aggaggagtt gttttgtcag
    13621 gaaacaaaca acacccaagc aaagacaaaa aagaagccag taaggcagct acagcagata
    13681 taatcgacaa gcggagaaat ctgcgtaaaa aagaagataa gatcgtgagg aagatcattt
    13741 tagtgatcgc tcttacatta ttgattattg gcggatttct cgggtttacc gtatatcgtt
    13801 atgtagatag cgggttgaag cctttagata aatcggacga ccaattagtc caagtggaaa
    13861 tcccaagcgg ctcttctaat aagcagattg gcgaaatttt ggagaaagac aatattatta
    13921 aaagtgggat tgttttcaat tattatacaa aatttaaaaa cttaactggc tttcaagctg
    13981 gttattatca gttagcgcct aatatgactc ttgatgagat tggcaagcag ttgcaagaag
    14041 gcggcacatc tgagccaaca aaagttgcag atgggaaaat tgccattcca gaaggctacg
    14101 acatcgatca gattgctgag cgggtagcga aggtaactgg taaagacaag aaagaatttt
    14161 tagatttagt aaatgatgag actttcttta acgagttgta tcaaaaattc ccagaattgt
    14221 tggaaagtgc ctctaaagct gagaacacaa aataccgttt ggaaggctac ttattcccag
    14281 ctacttatga ttactacacg agtacttcgc ttaaggatct tgtgattgag atggtgaaca
    14341 aaacaaatac ggtcatgcaa aactattatt cagctatcaa acagaaaaat ttgactgtac
    14401 aagaagtttt aactttggct tctctagttg aaaaagaagg cgtaaaagaa aatgatcgta
    14461 aaaatattgc acaagtcttc tttaaccgaa tcaaggcaaa tatgccactt cagtcagata
    14521 tctctgtttt atatgcatta ggagaacata aagaattggt gacgtatgaa gatacagctg
    14581 tagattcacc atacaatttg tataccaata ccggctatgg gcctggtcct tttgacaatc
    14641 caagcgaaga ggcaatcaag gcagtattag aaccagcaga aaatgactat tattatttcg
    14701 tagcagatac gagcactgga aatgtttatt tcgctaaaac gtatgaagaa catatggaac
    14761 tagtgcaaaa atatgtaaat aattcttaga aaaacagggg ctagtcattg atcattttta
    14821 gaaaaacatt tacattttaa gaaaagcgtg gtaatctttt ttggactata ttcctcaaaa
    14881 gattaccttt tgcggtattt tatcgaaaaa aggagacgtt acacatggta gagaaagttt
    14941 atccaatgac tctagaaggt aaagaaaaat tagaaaaaga attagaagaa ctaaaaacag
    15001 ttaaacgcgg agaaatcatt gaacgaatca aaatcgctcg cggtttcgga gatctttccg
    15061 aaaactccga atacgaatca gctaaagatg aacaagcatt tgttgaagga agaatcacaa
    15121 caattgaaaa tatgatccgt tttgcacaaa tcatcgataa tgacggcgta gatgcagatg
    15181 aggtatcgat cggtaaaaca gttacgttca tcgagttgcc tgatggagat gaagaagagt
    15241 acacgattgt cggaagtgct gaagcagacc cgttttctgg aaagatttcc aatgattctc
    15301 ctatcgcgca agcattgatt ggcaaacgta aaaatgatga agtaaccatt gctacaccag
    15361 gtggcgatat gttagttaaa atcgtcaaag tagaacaagc gtagtctgac tgaaagatct
    15421 agagagttca tcttactgat gacgcactct agatcttttc tctttgagta ttgcaggttt
    15481 cactgaacga tcttacatct aaagtcctat agccaacagt caaaaaaata ggtatgatga
    15541 aatgagaatg tgtcaggagg gagaaaaagc gatgaatagt tcttggcgtt tttttgtggt
    15601 agtcgaagca ctgctgctgt tatttgctgt ttggcaaatc gtaaacaata ctggactttt
    15661 gcttttagta atattcggta ttttcaatat atatctagct atgcgcaaat atcctcgtac
    15721 taaatttcaa aattttcaat tgatcttagg gagcttggtc atttttttta gcttagtcaa
    15781 cagtcctgct ttatggatga tggttgtatt ggccgtttta tttatcgggc tgaaaggtgt
    15841 agagatttca ggaatagatt tgacaaaaaa tgctttttgg cgaaaaaaac agatcatgat
    15901 ggtccaaaca gagcaattaa aaacacataa taatgagcgt aaaagacagc aattatttgg
    15961 taaccaacgt attggaaacg acgtctatga atgggatgat atcaacattg ccatcatttc
    16021 tggtgatact atcatcgatt tgggcaatac acttttgcca aaagatgata atattgtgat
    16081 tgtaagaaaa gggataggca gaacacggat cttagtaccc ctgggagtgg ctattcgttt
    16141 agagcatgcg acattagtag gaaatgtctt gtttgaagaa gagcagtttt ccttaaaaaa
    16201 cgaacagatc aaaatctgca gcagtgatta tgatgaaaat cctcgcagat tgaagatcat
    16261 taccaatact ttggttggag atatcgaggt gatacgaata tgatgggaag aatatccaaa
    16321 gcaatgctag ctgtttattc ggggattgcg gctttcctta ttattctatt ttcacttttc
    16381 acttattttt atgccagtaa ccaaagtcat tggtggggag aattgctgcg tgcgcgttta
    16441 ttatacgttc cgcttatctt ccatttgatg gcaatttcat taggtgtagg attgatcgta
    16501 tttctattgt tatcacttat tcaaaaggca aaatacggga aaatcgaaga aaagcttcgt
    16561 gcactttctt ctggcaatta tgaatccaaa cttttgcatc agccgatccc aagcgcgtcg
    16621 gatgatctgt acatcaaaga tattgatatg gaaattacta aaataaaaga aaaaatgatc
    16681 gaagtatcca gtgaattaca gattatgact agccgtcctc aatatgtaga tggacaaacc
    16741 aaagaagaga ttttaaaatt agaaaggcat cgattagctc gtgagcttca tgattcagtt
    16801 tcacagcaat tatttgcagc gatgatgatg atgtcggcat tgacggagca agcagaaaaa
    16861 agtgagacac cagaaatgtt ccgtaagcag ttgaaaatgg tggcagagat catcaacgct
    16921 tcccagtctg agatgcgtgc gctgcttctt catctgcgtc cagtcaattt agaagaaaaa
    16981 agtttaaaac aaggcattga gcagttattg aaggaattgc agaataaaat ccagatttcg
    17041 ctaaaatggg aggtagaaga tgtaaaatta actagttcca ttgaggatca cttgttccga
    17101 atcgttcaag aattgttatc aaacacatta agacatgcta aggctaatga attagaagtc
    17161 tatttgcata aaatagataa caatctttta ttacgtatca ttgacgacgg gacaggattt
    17221 aatatgaatg aaaccaaaac gggaagttat ggattgaaca atatcaaaga acgagtagct
    17281 ggtattggtg gaacagtaaa aatcattagt tttaaagggc aaggtacgag cgtggagacc
    17341 aaggtccctt tgatgaagga ggcatagata aagatgataa aagttttatt agtagatgac
    17401 catgaaatgg tacgcttagg cgtctcttct tatttatcta tccaagaaga cattgaagtg
    17461 attggagaag ctgaaaatgg acgtcaaggc tatgaaaagg cgatggcact tcgaccggat
    17521 gtcattttaa tggatctagt catggaagaa atggatggca tcgaatcaac aaaagcaatc
    17581 ttaaaagatt ggccaaaagc caaaatcatt atcgtcacaa gttttattga tgatgaaaaa
    17641 gtttatcctg caattgaagc aggagcagca ggatatttgt tgaagacgtc tacagcacat
    17701 gaaatagctg atgctataag ggctacgcaa cgcggcgagc gagtactaga acctgaagtg
    17761 acaacaaaaa tgatggaaaa aatgagtcgg cgaaatgatc cagtattgca tgaagaatta
    17821 acgaatcgag aaaatgaaat tttgatgctg atttctgaag ggaaaagtaa tcaggaaatc
    17881 gcggatgaat tgtttatcac actgaaaaca gtcaaaacgc atgtgtcgaa tatattagct
    17941 aagttagagg tagaagaccg aactcaagca gctatctatg cgtttaaaca tggtttggtg
    18001 aaataaaaac gatgataaag aggataatca aaagctgaga aaataactat tatttttctt
    18061 ggctttttta caataatgat gcttgctttt gtttttggaa aggctttgtt atactgagaa
    18121 aagaagatac taaaggagga aaatggtgaa acaaaatttt gcaattgtag gccttggacg
    18181 atttggcgga agcatctgcc gtacactgat cgaagctgga caagaagtac tggctattga
    18241 cagtaatgaa gatcgagtca atgaatatat gaatatagcc acccatgctg ttgtggcaaa
    18301 tgcacaggat gagatgacac ttcgttcatt aggtataaga aatttcgatc atgtaattgt
    18361 cgctattgga gaagatatcc aagccagcat cttagttact ctgatggtaa aggaaatggg
    18421 cgtaccgaat attttagcaa aagcccaaaa tgagtaccat gcccgtgtac tagagaagat
    18481 cggagcagat cgtgtcgtac accctgagcg agacatgggg atacggattg cacataactt
    18541 agtatcaaag aatattctag actacttaga gctgtcagat aaatattctt tagcagaaat
    18601 ccgagtctcc aatcctaaat ttttcaacaa aacattagct gaattaaatt tcaggcaacg
    18661 ttttgactta acagtagtcg ccatacgaag agtagatcaa acggttatcg cttctcctgc
    18721 agcagatgaa tatgttcgtg aaaatgataa tctactggtt attggagaga caggcgatgt
    18781 ggatattcta gacgataaga tgaatcaata gaaagaaaag agaaagtagg ttgttgggat
    18841 gaaattaacg gtaacgccaa aagcacaaaa gtggtttgac tcagaaattg atctgccaga
    18901 aacttacgga atacgttttt ttggaaaagt ctacggcaaa acagaggttc atgatggttt
    18961 ttcgattgga atgtcagttg aacagccaga acatcctgta aagaaagtgg aaatggatgg
    19021 gacacttttt tttatcgagg aagcagatga atggttcttt aaaggatatg atttacttgt
    19081 tgattatgat cccaacctgg aggagccaac ctatcacttt aaaaaacaat aaaaaaagag
    19141 agggtgacac aatttttgtc accttctctt ttttcttttg aaaatattgt tttcagctat
    19201 taatatcctg cagacaattt tacttgattg gtttgaagct catgggtggc aaagtagctt
    19261 ttcagattgg ttaaaaaaat agccattaat ttcttttgaa aatc
//
LOCUS       DAZKSU010000050        18906 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000050 DAZKSU010000000
VERSION     DAZKSU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.050"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..1528
                     /locus_tag="ACG8Z3_001832"
     CDS             101..1528
                     /locus_tag="ACG8Z3_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824140.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLHEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1620..1985)
                     /locus_tag="ACG8Z3_001833"
     CDS             complement(1620..1985)
                     /locus_tag="ACG8Z3_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0824141.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1982..2347)
                     /locus_tag="ACG8Z3_001834"
     CDS             complement(1982..2347)
                     /locus_tag="ACG8Z3_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0824142.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2539..2787)
                     /locus_tag="ACG8Z3_001835"
     CDS             complement(2539..2787)
                     /locus_tag="ACG8Z3_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824143.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2786..2914
                     /locus_tag="ACG8Z3_001836"
     CDS             2786..2914
                     /locus_tag="ACG8Z3_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0824144.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2971..4665
                     /locus_tag="ACG8Z3_001837"
     CDS             2971..4665
                     /locus_tag="ACG8Z3_001837"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0824145.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4732..5061
                     /locus_tag="ACG8Z3_001838"
     CDS             4732..5061
                     /locus_tag="ACG8Z3_001838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824146.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFL
                     TWLDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLP
                     RGYEKGV"
     gene            5439..6572
                     /locus_tag="ACG8Z3_001839"
     CDS             5439..6572
                     /locus_tag="ACG8Z3_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0824147.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6603..6923
                     /locus_tag="ACG8Z3_001840"
     CDS             6603..6923
                     /locus_tag="ACG8Z3_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0824148.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6952..7416)
                     /locus_tag="ACG8Z3_001841"
     CDS             complement(6952..7416)
                     /locus_tag="ACG8Z3_001841"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0824149.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7547..8800)
                     /locus_tag="ACG8Z3_001842"
     CDS             complement(7547..8800)
                     /locus_tag="ACG8Z3_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0824150.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8829..9146)
                     /locus_tag="ACG8Z3_001843"
     CDS             complement(8829..9146)
                     /locus_tag="ACG8Z3_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0824151.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9172..9492)
                     /locus_tag="ACG8Z3_001844"
     CDS             complement(9172..9492)
                     /locus_tag="ACG8Z3_001844"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0824152.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9956..11371)
                     /locus_tag="ACG8Z3_001845"
     CDS             complement(9956..11371)
                     /locus_tag="ACG8Z3_001845"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0824153.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11388..12704)
                     /locus_tag="ACG8Z3_001846"
     CDS             complement(11388..12704)
                     /locus_tag="ACG8Z3_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0824154.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12854..13552)
                     /locus_tag="ACG8Z3_001847"
     CDS             complement(12854..13552)
                     /locus_tag="ACG8Z3_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0824155.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13841..14830)
                     /locus_tag="ACG8Z3_001848"
     CDS             complement(13841..14830)
                     /locus_tag="ACG8Z3_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0824156.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14910..15743)
                     /gene="msrA"
                     /locus_tag="ACG8Z3_001849"
     CDS             complement(14910..15743)
                     /gene="msrA"
                     /locus_tag="ACG8Z3_001849"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0824157.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15918..17552)
                     /locus_tag="ACG8Z3_001850"
     CDS             complement(15918..17552)
                     /locus_tag="ACG8Z3_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0824158.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17539..>18906)
                     /locus_tag="ACG8Z3_001851"
     CDS             complement(17539..>18906)
                     /locus_tag="ACG8Z3_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824159.1"
                     /translation="SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIAL
                     TIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSK
                     QIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDS
                     IIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFL
                     FQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIA
                     AKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGE
                     SWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVD
                     VNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINK
                     KNENK"
BASE COUNT         5973 a         3734 c         3163 g         6036 t
ORIGIN      
        1 atttatatgc tttttcagaa attaactgat gatttcaaaa agaaaacatg tcataatatt
       61 tcccatattg tgcaaaaaaa tacaaggcgg aggctctact atgattgaag aatatattga
      121 gaaaaatatt ttaagacagc tgtttttatg tgggcaattc tatgtaaata aagaagtcaa
      181 tctagaaaag ctctccaatc tcctgcatgt ttgcaaaacc actttactca atgatatcaa
      241 taacataaaa aaagaatttg aagaacagat cgcttataca catcgggaaa aggaccgtta
      301 cactttatat ttttcagaac atatacctcg atgcaagatc atgcagcaat tgtctcaaaa
      361 ttcgttgttt ttaaaaactt gtctgcttta tttggaagaa gatgaacctg attatcttca
      421 attaactgaa tgtgaattca tctctgtcag caaagcttat tcattaaaaa aacaagtatt
      481 agcttatttt aatgactgcg ggatcgaaat cgaccgctac tcccctcgat tcactgaaat
      541 ggaaagacgt ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta attcttggga
      601 gctacctgaa tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag
      661 tggcagattt tatgataaag aaaataaaga aatcttatcg attggttttg ctatcagttt
      721 cctacgccag caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa
      781 acgtcctatt tacaattatg tcgaatcagc atgggaaaac actgatttcc aaacctatta
      841 taaaaaagaa gaattcgcct ttattttgac actattcaat ctttgtaatt atggtttcca
      901 ttcttatcag ttgattgcgg aagactttca acagctacac caagtgttta ttgacaatac
      961 tcccgaaata aaagaactag ttgctacctt tgaatctcac ttcaatcaag aattattcgg
     1021 aaaccaaccg ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg ataactatca
     1081 attattcatg ccagaaaaat tttatctttt acatgaggaa cagacaaatt tatataaaga
     1141 agtccagaca attttttcca gctggtccag ccagttacct tatgatctac ggttgaatcc
     1201 taattgcatg cgggcttttg tgatcgaatt gagcggaatt ctccgtctga caaaagaaca
     1261 tctgactatc tatatcgtca caaattcgga tgttcattat ctgatttatc gggaagcctt
     1321 ggaagctgta actactttcg attttcaagt tgcaccaaca atttattcaa gtatatctga
     1381 tataaagaag tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta cattatatac
     1441 accagatgcg gtccaatatg aaaatattat tcctatttcg atcaatacga tcgatcgagc
     1501 tatcatttca gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt cacagcctct
     1561 tatttgttga agatgcccat ttctgtaaca atctcttgat acacgtcagg ctcacaacct
     1621 cacttatttt ggacatctac gggtccgatc tctttattta aaaaataaga aatgatggtt
     1681 cggataacta ccaacgccgc tagttggaaa atatcctgca aggtaggttt gataatcgat
     1741 tcgatgatcc cagctgaaac aagaatttcc aaacttagaa gtatatagcc tcccaacatc
     1801 tttttaatag cattgttctc gctgactcgg tcgctttgca cttttgtatg gacaaataaa
     1861 tatttcaaaa aatccttgat tgatagacaa acaccccaaa ccaataaaca gatagcaaag
     1921 atatcaagtc ccagtacaag tttttcgaaa tatggaagaa gaaactgcat caactctgtc
     1981 gtcatcctac ctctcctctt ctttgttttt ctttgtcttc tatttcctga ttcaagaaat
     2041 aagaaatgac tgttcgaatg ataataataa gtgcaagctt caaaatatct gtccaagtcg
     2101 gcttgataac tgactcgatg atatcagcaa cgactagtac ttccaagctt aacaaaatat
     2161 aacttgctag cagttttctt gtatctgcat tacgagtatt acgttctgca tagtttttct
     2221 tgacatgaga ccaatcaaaa agtttgaata gaacaagaac gatcccgaca attaagatac
     2281 cgatagaaca aatatttaaa atagcaatga tccattctag aaattcaatt aaatttgagc
     2341 gaaacatata cgctacctac tttcttttaa tttgttccta ttcttttatt gtaaaagaaa
     2401 gttaccttgt tcgtctattt aaaagttgtt ctaatgataa cggttcgctt aaatttttct
     2461 ttttcactct cccatttttc tccctccatc cataaatcgt ggaagtatcc aaaacaacgt
     2521 agcaagttaa aataaggctt agtccgtcat cagcttttca aagaagcact gtctcggtgc
     2581 tttttttgta cccaaatttc taaaaataga accatacacc tcctcaaatc ttttcttctt
     2641 acctacccta tttttattta caggaaaatc agacacttct tcttttttgt caaaatttcc
     2701 tttttcttct cgtcctatca tttggcattc taataaaaac gaactatcct tggatataga
     2761 aagagaaaac aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg gcatcggttt
     2821 aggtttgctt gcccttggcg ctgcatatct aggaaaaaag acaggactat tcgaagatga
     2881 cagtcattta tatgatgaat tcgaatcaat ctagcatcct atttagttga atcactacgg
     2941 acatacttgg aagacattgg aggaatcaca atgagaaaaa gtaaagcaat aatgattggt
     3001 gccggtattg ccaatatggc agcggctgtt tatttgattc aagaaggcaa atggaaaggg
     3061 gatcagatca ccttttattc attggatgat cacggttcaa atgatggtgc accgaccgac
     3121 actgtagtgg atgaatattg gaataaaaac catccattag aaaaccaaaa aggctatatt
     3181 gctcgaggcg gtcgcatgct taattaccgt acctatgtag atttgatgga tctgttaagt
     3241 cgaatacctt ctgttactga gccagggatg actgctgaag aagatacgag agattttgat
     3301 gcaaaacacc gaacctttga caaagcacgt ttgatggaag gtggcaaagg aatcattgat
     3361 ggcggaaaac tgggattcaa taataaagac cgtattcttc tgactaaact gattgctatg
     3421 cctgattcag aagaagaaaa actagacaat atcactatcg aagaatattt caaagatgat
     3481 ccacactttt ttgaaacaaa cttctggttt atgtgggaaa caacatttgc tttcagaaca
     3541 cgtagttctg ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga attcactcaa
     3601 atcgaacatc tagtgggcgt caatcgaaca cgttacaatc aatacgaaag tattatgctt
     3661 cctttgatcg agtacttgaa agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact
     3721 gactgggaat tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt
     3781 accaatacac tgactcaaga agaagaatac gttgtggtcg atgaagatac agcagttatt
     3841 ttcactaatg ggtccattac agattcagct actttaggag attacgaaac accagctgta
     3901 gaaaatatgg attatggtgc agcttctagt ctctggaaaa aagccagtga acgtttctac
     3961 aacttgggaa atcctgacaa attctttgct gatcgagatg caagcgaatg ggtcagcttt
     4021 accctgacga cgaaaaacca tttgttgttg aatgaaatca cccgtatcac gactcaagaa
     4081 ccaggaaatg ccttgaattc atttatttca accacaccga tcacgccgtt aggccaaaaa
     4141 gatgtcaata tgtctatcgt cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat
     4201 gaaacagtta tttggggtta tttcctttat ccaagacgac gtggcgaatt tgtggataaa
     4261 cagtatatta aaatgaatgg aaaagaaatg ctagaagaac tgattggaca actttcaaaa
     4321 gtagatcctg gaccagtgaa tattcgtgaa aaagaaacag aaattttcga cagcgtaata
     4381 aataatatcc ctgtctatat gccttatgct tccgcgttgt tcaataatcg tgccaaaagc
     4441 gatcgaccaa aagtcattcc taaacattcg accaatctgg cctttacagg agaatttgtc
     4501 gagcagcctt atcagatgat ctttactgaa caaagtgctg tacgttctgg agaaatcgct
     4561 gctttccatt ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa
     4621 gatattccta ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa
     4681 aaagctgccc tgcatcaagt aataagaaca accaggctaa aacagcttct tatgtttttg
     4741 atttggttgg acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttgt
     4801 gaaaaagctg ccctgcatca agtaataaga acacccaagc taaaactgct tcttatgttt
     4861 ttgacttggt tggacttatt accgcagatg caagcttttg aacaccattt accaagaggt
     4921 tatgaaaaag ctgccctgca tcaagtaata agagcagcca agctaaaaca gcttcttatg
     4981 tttttgactt ggttagactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga
     5041 ggttatgaaa aaggcgttta gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc
     5101 acgtttttgc ataaatttga cttaataccg agaaactgat cccttctgtc ataacctctt
     5161 tttatttatg tctgtttctt tttgaaaaca actatttttt caacgtttac tctcttgcca
     5221 atccaacatc atctaacgga tacaactatt tcaatgatta ccattacact aaaaaatcgg
     5281 aaacacacct ctccgaacac aaatacataa gactatagaa tgtttcattt aagaaaaaaa
     5341 gcgtttcatt atcaaaaagg taagatctcc tttaaaatga aattatctgt agtactaata
     5401 atgcagacaa ttttatgaat ctgaaaggag catctccaat gaaagcagtc acttggcaag
     5461 gaaaacagaa aatggaaatc agaaacgtag atgatccaaa aatattagaa ccaacagatg
     5521 caatcatccg tatcacagca acagccattt gcggctccga tctgcatctt taccatcatg
     5581 gtgaagcagt tttggaaaaa gggtatgttg taggacacga accaatggga atcgttgaag
     5641 aagtgggatc tcaagttcaa acattgaaaa aagggcaacg tgtggttatc ccttttaata
     5701 ttagttgtgg tcattgtcat ttctgcctga atcacatgga aagccaatgt gatgaatcaa
     5761 atccagatca gttatatggc gggcttttcg gatttggcaa gttgaacggt aatcattggg
     5821 gtggtcaagc ggaatatcta agagttcctt atgcagactc tacttcattt gttgtaccag
     5881 ataatgatct tcctgatgaa aaagttttat ttctatcaga tattttgccc acagcttatt
     5941 ggagcgttga aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc
     6001 cagtcggtct attcgctcaa aaattcgctg ttatggctgg agcagagcgc gtaatcgcag
     6061 tagatccagt acagcaccgt ctagataaag cggctaatta caatggggtt gaaacttatt
     6121 tcttagaaga cacggcacaa gctggaacag aattgtatga actggctaaa ggtggcgcag
     6181 atgtcattat cgattgtgta ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa
     6241 atttagtaag cttacaaagc ggcacaatct ctccattaca gatggcatcg caagctgtca
     6301 aaaaatttgg aacagtccaa gtaactggtg tttatatgac tccagcttct tcttacccgc
     6361 tgcaagaatt ttttatgcgg gatatcgctg tcaagcatgg acaggcacca attatccatc
     6421 taatgccaaa aatttacgca atgattgcag aaggactatt cgatcctaca caaatcatta
     6481 ctcattcgct gccactatca gaagcagcac aagcatatga gatctttgac aaaaaagaag
     6541 ataaaaatat aaaagttgtt ttgaaacctt gaattcagta aaaaatacag gtgattttct
     6601 ttatgcccaa aatcaaacga aatacccatg agatcgaaat aggaaaagaa gaagatatcg
     6661 aaatacgagg acgtcgcttc cgcttctatt tccaaaatcg ttacacattg atctcactgg
     6721 ctgtcgattt attaaccggg attttctata tttttggaag ccttgcttcc ttaactaaaa
     6781 tccccgatcg atatggcacc tatttttatt tggctggtgc tattttcttg acgattcgtc
     6841 ctattctcag aatcgtaagg aacattttta tttacgacga aaaaaaagca atgaagagaa
     6901 caagcaaaaa aaaagaggca tgagaatttt tcaaatctca tgcctctttt ttcaccctat
     6961 cgtatcactt tgatacagac gagcggtcgt cccactttct tccttgacca ttgtcaaaaa
     7021 tgtccactcg tatcgttcat aaaacgtggt atgatctgta attaagtaaa gtttttcgta
     7081 cccaagttga tgcatttctt ttctgatttc cgctaatatc cgcttggcta tcccctgctt
     7141 acgcttttcc ggttcaacaa acagggcaca aagatttggt gtcagatcag ggcggttatg
     7201 gaagtcgttt tcaatgacgc ccgctcctcc gacgatggtt tctttttcat ctaagaccac
     7261 ataccattga ggaatacttt ccttattagc gattccagta cgaatacttt cctcatacac
     7321 aacagcagga actccccatt ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg
     7381 gtgttctctt aatttgatcc agttatatgt aaccaatgtt taaacctccg actaaaataa
     7441 aacgtttaca aaaattatac aagtctttat gaaaattagc aatgacaaca atagtgatgt
     7501 aaaaaaaaaa cggaaaacaa tcacttgttc tccgtttttt gattaatcaa gcatctaaag
     7561 cttcttttct tctttcttgg atcgtagcta ccttgacgaa cggtagatag atcaaaatag
     7621 aaagaaccaa attgacagcg gctagtacac cacctgcaat actttttgtt gccaatacac
     7681 cgccaataat tggtggagtt acccatggag gcataacggt agctgctggc actaaacctg
     7741 tagaagtcgc aaagtaagca acactaacta agaccattgg tgtcaaaata aatggaatga
     7801 acatgatcgg gttcaacacg ataggtaacc caaacatcgt tggttcatta atattgaaga
     7861 cacctggcgc aatcgacaag tttgttacaa ccatatatgg tttgttttta cgtcctacta
     7921 aataaatagc taacagtaaa cctaaagttg cacctgttcc tcctaagttg acaaagctgt
     7981 caaagaaagg tttgttcacg ataaacggtg cgctatgccc agctgcgatt gcattaacgt
     8041 ttgcttcaat ggctggcgca ttgattgttt gcatcaaagg atcgaccata ttcgctccgt
     8101 gtaaaccaaa gaaccataag aatggtgtga taaatgcaag cagtaaggca gatggataag
     8161 agtttgcaag tcccataaat ggttcttgga ccactgtata gaaagaacct acgatatcat
     8221 caacaccgaa tgcagcaaaa attgcagcaa ctaaagcaaa cgcagagata gtgatcattg
     8281 ctggtagtaa tgaagcaaat gcttttgcaa ctgccggtgg cacaccatca ggcattttga
     8341 tgatcaattt ttcatttccg cttaaacgta caaagatttc tgttgaaata atcccaacaa
     8401 taatagcaat aaacaaaccg tttgagctca tacctaatgc tccgcctaaa gcgaagaaag
     8461 agcctaaaga aatcacacct gcggaaacac cgtcttttcg gtagccgtta gctaaatgat
     8521 aagcaagcaa gaaagcaatc aagacagaaa gaatggcaaa tgtaccgttc caagcagcag
     8581 ccccgaatcc tttccatgat tcaccaccaa aaattgagtt catcaattct tggaacccgg
     8641 gaatcggtaa gttattgatc attactgcta atgctcctaa gatcattaac ggcatactca
     8701 ccataaatga gtcccgaatc gcaactaaat gccgttgtgc accaattttt gatgctacag
     8761 gtataaaatg tttctccata aatgcaacaa atttatccat aaatcttccc tcttttcttt
     8821 actgtagatt acgcgttcaa tcgacgatac agatcaatga tctcttttgc taaatctgta
     8881 aaagctatag atgtcatcag atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg
     8941 tgatttcctt gtgcttcttg tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa
     9001 gattcctctg catctttgat tttttcatct gctaaagtga aatcaccttt tttcgcagca
     9061 gcaatcgctt ccattgcatc gctttttgca tttccgccaa acatgatcag acccatgatg
     9121 gcttctagat ttttttgatc ttccatttat tgtatcctcc gataattttt attatttgtt
     9181 tattagtttt tctgcttggt cgagtacttt ttcaccattc atcatgccat agtcagccat
     9241 attgattaca tctaaaggaa tgcctttagg tgctagcttt tcagcaaatt gattttgcat
     9301 aaagcgtact tgcggtccta aaagtaaaac gtctacattt ttagattcta gttgattatc
     9361 cgcatctgaa gcagatacag caaagatgtc tgcttccatt ccttttgctt ctgctgcctt
     9421 ttgcattttt gtgactagga gacttgtact cattccagct gaacatacaa gcatgattgt
     9481 tttcttttcc attttaaaca cgttcctttc tatacttaca cttgtatata agcaagtagc
     9541 gtgccaactt taaaagcgct ttaaaaagtt gatatcatca catttaaggt ctataccaag
     9601 ttgcatacac actaaaaaag aaagcgcaca ctaaacacac aaattaattt ttattacctt
     9661 ttttctttta acgtgcacaa aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa
     9721 taaacggtat tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacataag
     9781 atgttgtttt tactttgcta ttcttattac ttgatgcagg acagcttttt cacaacctct
     9841 caataaacgg tgttcaatca gctgaccctg ggtattagct caaaacgctg gattcacaac
     9901 ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt
     9961 tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag
    10021 cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc
    10081 gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt
    10141 cgcaatttgc gccttcttct gttgctttga tcaagtaatg caaatgctgt ccgataaatt
    10201 cgatacggta atcatcttgc acgattcctt tctcatccat atactgttct tcatattctc
    10261 ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct tttagataca
    10321 tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct
    10381 tcccaggtaa atcaaagact tcataatatc gttcaggatg gaaaggtgtc tcttcattcc
    10441 attgataacg aggggcgcga acgcgtttag gataatattg attgattcca aggaaatcta
    10501 ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt
    10561 tttgacacaa ctccaggaac tcttctgtgt agtatccttt taccatcgga tcaaaaaata
    10621 ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga gaattggatc
    10681 gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc
    10741 ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac atcatccact
    10801 tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa
    10861 cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaaaggcaa
    10921 tttgagcata cttagcataa aaatcaacta cttttcttga agaccagcca tcatattttt
    10981 ccaaaagaaa agctgggact tcataatgtt ccaaacaaat catcggttct acgtctgctt
    11041 cgatcaaaga gtcgatcatt tcacttatat gctgagcata ttcttcatct actatacagt
    11101 tctcataatc agtaaaaaaa cgagcccaat tgatagaagt ccgataatgt gtcagatgga
    11161 tggatttcat cagatccaca tcctgctggt attgttccat aaaattagtt gctacggctg
    11221 ggccataccc attatgccaa acaaaatgtt catttttata ccagctatct ataaacgaat
    11281 cctgtccttc tttttttcct ttccaacctt ccgtttgcca agcagaagat gcagcaccaa
    11341 gaataaaatt ttttggaata gtaattttca tgatttcctc ctgtttttta gatcccatca
    11401 ttttccacac gatttgctac ttttacaaat ggcagataga tcaaaataga aacaataata
    11461 caaacaagtt gcgtaataac tgcaccgata tttcctgctg ttgccagata agcattgacg
    11521 atcggcggca ttggccaagg gaccattaca actgctttcg ccgcaaatcc taaagcggtt
    11581 gcaaaataac caatcgttcc agtgataaga ggcgttaaga taaacggaat agctaaaatt
    11641 ggattcagca tgatcggcat accaaaaata actggttcat tgatgttgaa gatcccagga
    11701 gcaagggata acttggtgat ttgtttcata tcttcccgtt ttccaactaa caatactgca
    11761 atcagtaaac cgatcgtgac tccagacccg cccatactca tatacatatc ccaaaatggc
    11821 atagtaataa tgttcggtac ttcttttcca gcttcaaaag cttccgtatt tacagcaatt
    11881 gccgctaaaa gcaacggttc ccgtatcggc ttcaccattt gatttccatg gataccaatc
    11941 acccaaaaga cttgcgcaat aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca
    12001 ccttctaatg ggcgttgtac gacattgtaa atgatatcat aaaggtacat tcctgtaagt
    12061 tctttgatga taaatcccgc agccgcgata cccgatacag tcagaatcgt cgggaataaa
    12121 gcagaaaaac tagcagaaac attcgttggc acggtatcag gcattctcag ctgtagtttc
    12181 ttctgtttcc tcaaccagca ataaagttca atagaaagga tggagaccaa cattcccaaa
    12241 aataaccctt tcgtatctgt atattgttta gccaatacgt tttctactat aacagtctga
    12301 ttttcattca ctaattcata aacagttgga ttaacagata aataagacat cactgcgatg
    12361 ataccaggga atacaccatc cactttattc agcttggcta attcgatccc tatcagaaaa
    12421 acagcataga tggtcaagaa acttagtgtg acatagctga tggcagatgc gataggcttt
    12481 aattcttcta aaaaacgcaa gaaacttatc tgtgctaagc cattttccga gtcaaagacc
    12541 attgctgaaa acaatgtgcc aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat
    12601 gcgtttttta tcaccatgat ataccttaaa ttattgattc tcgttgccac gttccctaaa
    12661 acattggcta atttatccat aaaactcatt ccagatcccc tcattcctgc ttcttctcct
    12721 tgacgtcatc tctttttgtc tattagtcag cgcttacatt tcgatgaaca gaaactattt
    12781 actcctcttt tttaccaaat ataaaacaaa aattcatctg atgtatttat cataaaatac
    12841 atcagatgaa ctgtcaatta gtttcttttt ctcctattat tttctgtaat tctctccgat
    12901 ttattgccat atggacagtc attgcatcaa acgcagcaag tgcattccgc ctttcgatgg
    12961 cctgaacgat ctctgtatga atatcgatcg tctcgatttt cagatgctgg atatcataat
    13021 ttttattaaa caaagcaatt gattcattaa tgattgggat aatatgcagc aaggctacgt
    13081 ttccgctagc ttctgcaatc atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg
    13141 tgccttctcg aaaagaagtt tcaatctttt gcgttaattt tttcatttgg acgatttgat
    13201 tcttcgttgc atattccgct gccaaacctg ccatcttagg ctccaataaa tatctcagtt
    13261 caaataagtc tcgtatcatc cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat
    13321 tggcaatccc tcgcttatca ctgacaaatg tccccgctcc ttgccgaact tcaaggatat
    13381 tcctggaagc tagagcccgc acagcttctc gcaccgtgct tcgccccacc tctaaataat
    13441 cggacaattc atattcattc ggcagtttat ctcctacttg caactgcatt tcctttaaat
    13501 atctaaggat cttgtcagta gttacttcta ctagggtttt attttcttcc aaaccacata
    13561 cctccttttc atctgatgta ttttttcgta attattataa cacgtttgaa tcatctgatg
    13621 attttttgta tacaaaattg attaatccgt tcatagacat acaaacgaag aagatattta
    13681 ccataaaaaa gactaggata aaattcactc ctagccttcg atcattttta ttttgaacga
    13741 caaaaaatat gatgttcaat aacagacgat cttcattcga ttcttgatgt catcttttgt
    13801 tttttttaat atatggataa aaagtttgat tacatcgttt ctattctaga atttggtcga
    13861 ttctcaattc ttcttctggt gagaaatcta aattatccat cattcgaaca ctttcttgta
    13921 actgacttaa ccgacttgct ccgaccagta cacttgtaac agatttttgt cgcaaattcc
    13981 atgcaagggc catttgcgct aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt
    14041 tgattttttc cagagttgat ccaacttgtt cttctgaaag gaatggtatt tccttacgat
    14101 gtgctctaga atcttcagga ataccatgta gataacgatt tgtcagcaac ccttgagcaa
    14161 gcggactgaa cgtgattgcc cctagccctt cttcttctaa tactggagat agatcgtctt
    14221 ctagtagtgc acgagaaaac atattgtagc gcatctgatg aataatgaac ggtgcttcct
    14281 ttctttttaa aatttcagtc attttttttg tatcttctcc attatagttg gatataccaa
    14341 tatataatgc ttttccttgt cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag
    14401 gtgtatcagg gtctgggcga tgatgataga agatgtcaac ataatctagc cccattcttt
    14461 ggagactttg gtcacaactt gaaatgatgg atttacgaga gccccattcc ccatatggcc
    14521 cttcccacat atgataacct gctttgctag aaataatcag ttcatcacgg taggcttgaa
    14581 aatcttctct aaaaatacgc ccaaagtttt tctctgcagc tccagcaggg cctccataat
    14641 tattcgcaag gtcgaaatgg gtaattccca tgtcgaatgc gcctcttaaa atctctcttt
    14701 gattatggat aggatcataa tcaccaaaat tttgccataa tccgagagat aaaatcggta
    14761 atttcaaacc tgaatgaccg gatttacgat attccatttt ttcataacgt gcactatctg
    14821 caatatacaa tttttctcct cctcatcaaa tctttttaaa acgctttctt ttttctattt
    14881 ttagtttact acattttaaa gaaaaggagt taatcaaaat gattttttcg tatatttttt
    14941 tgccatttgt tttttactcg ttgatagatc agaaattgat tacgtgcttg ctggattttt
    15001 ttataacgtt tcggttgttt tttgtaaaat tgctgatgat agttttctgc tggccaaaac
    15061 gtaccggctg gttcgatttt tgtgacgatt ggttggtgga aagttcctga actgtctagt
    15121 ttctgttttg attgttccgc taattctttt tgtcgttcgc cttgataaaa aatcactgga
    15181 cgatattgct tccctctatc ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc
    15241 caatagattg tcagcaactc gctataactt atttctcttg tgtcataaat gatctccaca
    15301 gcttccacat gacctgtgta accaccactt acttgatcat aatttggttt ttcaacatgt
    15361 ccgcccgtgt atcctgacaa aacagatagg atcccttttt tcgattcaaa cggctcgacc
    15421 atacaccaga aacatccccc tgcaaatact gcacgttctt gatagtcagc atcttgcatg
    15481 gcatgcttgg aaaagtcata tttttgctcc ttatcaaatc cttccgtaat caataaataa
    15541 aagtccatta catcaggcgt cagattcatt cggatagcta atggacgtag tgttgcttct
    15601 agttttgata actgttcttt aacggatata ttttctctta tgtcattctt tgtctgaatc
    15661 aatacttttc gttcccaatc tcgtgtattc ggattcaaaa tcagattata aagtgtttga
    15721 atgaatattt gttcctcatc catagattct cctcctttcc gaagaagaca agctagtgac
    15781 tttagctgta atttatcata aagtgaggaa ataagcgatt acttcatccc cctacagatt
    15841 tttcgtataa aaaagagaga atccttatcg acagattctc ctttttaatc aatttatttt
    15901 ataaatgaaa agttaattta ccttttatgg aaaactattt taaaaaataa acataaatat
    15961 gcagttaatg taacaagata agaaataata gtcgttactg tataacttgt gcttacttta
    16021 atatcagcaa ctcctttgac tccgcttatt tgtaccaatc cattagcatt cacctgctcc
    16081 tttacaggtt gatgatttat ttctacattc gttcctaaat aagcatatac aggaagtgtg
    16141 attacttggc tcttctcatt agttactcta tatatgatag aactaccagt tgttttataa
    16201 ggaaggttta cccattgact gccaatcttt gcttcgtgtt ttattatatg ctccttattc
    16261 tccaatgaag cgataggaga ataatcatat aaaaaataat ccattccttt agtggtatac
    16321 gcctctactg tttctttgtt tacaacaata tgtcccggct gagaaattat tttttgattg
    16381 aaattttttt tactagaaac agttaaagtg attaataaca aaacagtcat aaataaataa
    16441 aatagttctc ttgtcttatt gtatcttcta tttttgaaaa atatcatcgt taatatcaga
    16501 gaagagaata ttatttgcaa gcttaagatc cgccaaggaa attgtaacaa tttggctggc
    16561 gtattttgca gaagatacca tggcaccaaa ctagttgtag ctagccaagt caaacacgta
    16621 ccataataaa tctttttttc tatgtttgtt aattttttaa ataaaatcag gggaatacat
    16681 aaagataaca gaccaactag tcccattgag taagtagtta agtcgttgtt catagcttca
    16741 gtcagactat cgaacacatt agatgcatga gcatataaat ttgtcacgct aggtttgttg
    16801 attttttggt agagcatttg ttcaaacatc ggataccaaa aagcactcgt cagaaccaat
    16861 gtcattcctg ccgcactaaa taatttttta aatatagcaa taaaatctgc attcttgaaa
    16921 cctttagaaa ccaaacgaat aaaaataaaa atgataatta atgctgtact cattaatgtt
    16981 gatagtacat ggctgtacac tagcaaactc atactcaatg ctagctttac agacggatac
    17041 ttttcttctt ttccagagat gatttgataa gcataaaata atacagggat aacaagtgtt
    17101 attgcgatag cttcggcaat tgcggaacga tagtagatgt ctactgtccg ataagcagag
    17161 aaaatataga gacaactaaa taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca
    17221 taatgataag ctacgagtgt tgtacacaag ttcaataacc aaacgtaaat aatatagctt
    17281 acaatcaaat tttttgtcat ccaatataac attacagctg gaaaaaaagt cagaaaggga
    17341 taaaaataat ttacaccata tccagtataa ttgaatgtct cataattaat cggactggta
    17401 aaaatagtcg ataacccctt gattcgagat aagtgaaaag aaagatctcc agtggtataa
    17461 atactctgtg tatttttgaa aaagatgaaa gaatatatac tactaactat aattaatcca
    17521 ctcaatatta ctactttttt atttattttc attcttcttg tttatctcct ttttagtcac
    17581 atatcgagaa tctttcaact tattaggtgc aaatcgttta aaacttgaat acaccactaa
    17641 acaataagtc agaatactga tccataaagc catgagagca gttggagtat attttgttgt
    17701 aatttctatc ttattttctc cagcatttac atccacattc actaagccat gttttttata
    17761 agagtcattt acgactttac cgtttacttt tacctgtgta cctaaataag catatactgg
    17821 tactgtcact ttttgctttt tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc
    17881 tttatacttc cctttttccc acgattctcc tacttttact tcatgttttt ctaagtgagg
    17941 cctttctttt atcgcttctg ttggggcgta atcgtataat cctcccatat ctgatgttgt
    18001 ataacgtggg agtgtttctt cattgataac aatatgaccg tgttttgatt ggataacttt
    18061 tgcgtaattt tcttttgctg ctatagacgt gacgagtaga aacaccacag tagcgccaat
    18121 gattaaccat gaatattttt tattcatagt ctttttcata aaaacaagcg ttatgatcat
    18181 tgaagaaaaa aagatttgga tacctaagat acgccaagga aactggagca agcttatcgg
    18241 tgttttttga aacaaaaacc atggaaataa attagttacg catacccatg tagaaactac
    18301 gccaacatag atgatttttt cttttttctc catttctttc agtatgaata aaggaaaaat
    18361 caacgcaacc aatcctaata accccatagt gtatgttgta atatcgttat ttaacgcttg
    18421 aatgatacta tctgaaatag aaagtgcttc tagctgcaaa tctgttttga agggactgtg
    18481 tatttcttga taattcatct gctgcaacat aggataccaa aaatagctag ttaatgcaca
    18541 agtcaaaaaa acagccttta ctaaattcac gaccacaaat tttatatcgt tttttatttg
    18601 cttgcttaaa aacaaacgaa ctataatcat acaagcaatc aatgcactac acaatagtgt
    18661 agttaagaca tgagtataca caagtagact tatactgaca gataaagcga tgactttttt
    18721 ttctctattg aatatcaatc cataagcata atataaaaca gctgggataa tcgtaagagc
    18781 gattgcttct gcaattgcgg atcgataata gatatcaact gttcgataag ttgcaaaagt
    18841 atataaacaa ctaaataaaa aagcagcatc tatcttttta aaaaactttt ctccataata
    18901 aaaact
//
LOCUS       DAZKSU010000051        18504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000051 DAZKSU010000000
VERSION     DAZKSU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.051"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8Z3_001852"
     CDS             99..746
                     /locus_tag="ACG8Z3_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0824160.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8Z3_001853"
     CDS             1139..3784
                     /locus_tag="ACG8Z3_001853"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0824161.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8Z3_001854"
     CDS             4094..5407
                     /locus_tag="ACG8Z3_001854"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0824162.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8Z3_001855"
     CDS             5397..6050
                     /locus_tag="ACG8Z3_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0824163.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8Z3_001856"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8Z3_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0824164.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8Z3_001857"
     CDS             6947..7147
                     /locus_tag="ACG8Z3_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0824165.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG8Z3_001858"
     CDS             complement(7401..9080)
                     /locus_tag="ACG8Z3_001858"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0824166.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG8Z3_001859"
     CDS             complement(9082..9294)
                     /locus_tag="ACG8Z3_001859"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0824167.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8Z3_001860"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG8Z3_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0824168.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8Z3_001861"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG8Z3_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0824169.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG8Z3_001862"
     CDS             13269..13466
                     /locus_tag="ACG8Z3_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824170.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG8Z3_001863"
     CDS             13619..14104
                     /locus_tag="ACG8Z3_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0824171.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8Z3_001864"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG8Z3_001864"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0824172.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG8Z3_001865"
     CDS             14981..15508
                     /locus_tag="ACG8Z3_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824173.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG8Z3_001866"
     CDS             15599..16336
                     /locus_tag="ACG8Z3_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0824174.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8Z3_001867"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG8Z3_001867"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0824175.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8Z3_001868"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG8Z3_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0824176.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3200 c         4022 g         5298 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZKSU010000052        18337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000052 DAZKSU010000000
VERSION     DAZKSU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.052"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..197
                     /locus_tag="ACG8Z3_001869"
     CDS             <1..197
                     /locus_tag="ACG8Z3_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0824177.1"
                     /translation="NNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRN
                     GTLFGFSVSTEIKVLMGITA"
     gene            980..4633
                     /locus_tag="ACG8Z3_001870"
     CDS             980..4633
                     /locus_tag="ACG8Z3_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0824178.1"
                     /translation="MKKKKSVWFMMVAILMGFIPQILTAYSSIAQAIETQPDKIVDQS
                     GLKVSSSVSSDGNSLNWELQYEKSVASDGNDQALKFKVTADGEAIAFNEDASFAVDDD
                     QWFAEKDFSKQSQGSLSFTTVLNVSKVALEIQADATKTDGTQAVTINKNILTSAVEGP
                     HALKLPAVATQKTTEVSATSQTTAEETTSSQATETTTQTEEASEAKSSEINASPATVG
                     ASNLEYLSGISAFSSINYSNIAPEYTTDATKGTYPTNSWQPAGQTNVINHQGNKERNF
                     ATWDGVTSWNGDPSDTTHSYIQYGEKQTDFAIRKYAKETSKPGLYDVYLNVKGNKQED
                     IKPVDIVLVVDMSGSMESSQSNGWNDRAGAARNGVKNFLQTIKDAGIGDYVNVGLVGF
                     SSPGYVTGPNGYLTVPIGKASDTSHINAINDALKPKFTGGTYTQIGIEQGQQMLAGSS
                     NENKMMIVLTDGVPTFSKKVTAAQTIDGTTYATEFGNRLDEPSTTSGLPWWDRYTVDR
                     TLINSTWPATLGAAKIAKDAGLTIHLLGIQLSKDRNFLTEQQVRDRASLIATPGKYKD
                     AETTDDVSDYLNEQAKNVVKSFNTIVNGSINDSLGDQFSYEGTPTVKSISTGDSAVTN
                     LPTVNQSNGKVTTTELNLGKDQEIQIHYQVRINTEDKDFTPEKWYPMNGQTTLTPNGD
                     NPDNKVDFGVPSAKAPGVKLDLKKIWEEYDKDTSKRPDSLNFTLNRKDTTESNSWKQG
                     FIRLSKSDVATDTNVWEKTNIEKVAETQGATESLWLPQFNNKGKDFAYSFEEATVNGY
                     ESSSNAATTIWTNKKIYTPLALEITKISDQGKTPLKGAVFKLSGGSLPNAGVELKDNG
                     DGTYTLSDDKYKLQLNTEYTLTEVTPPAGHTATTTSWKVDVSAEGKVTINDAVPGIEI
                     KDNTIKYTIENEFTKRPLLVEKYNKDSGKTLDGAKFTLKQYDDKWTNDGKVVGDDLIG
                     NDAKSFASLAPGYYSLEETKVPTGYKKDDTVFKFQIDSAGKLLDANGKEITANSKPTT
                     DGFYLTSDNKIVLIKYNELRDFDFSILKKNSQSNKPLADTEFSLATKEDSNTILATLK
                     TNDEGTGQFLDASGNHYGVRPGTYVIKETKAPEGFVLLKGIFEVTINADGTVGDVTYD
                     GKDLDKDAIKVNLKDEDNNVIELTVANTPKGQLPATGGSGIQTFIIVALALVTAAGAL
                     TICYFYRNRKELN"
     gene            4635..5366
                     /locus_tag="ACG8Z3_001871"
     CDS             4635..5366
                     /locus_tag="ACG8Z3_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0824179.1"
                     /translation="MKNKSLFIKITILLSTLLVGIFTGQQAFADDGTIDIVIHKQLWD
                     QAPDAAIQNTGAADQDIKDPIEGVGFTIYDITEDYYKLVKGSDETTTIQMIQENVADY
                     QKIIREQGLTDEKSTIKFTQLPKSSGGKNAVYLIVETALPDLEKVKVEKAAEPLVVAL
                     PVYEMSTDGKTYTDKELATVHLYPKNVGTIVEKPVTPTKPKKPSKPKKHLPQTGEAKS
                     FLGILGILLIGTAIILWRKHSVRNN"
     gene            5431..6918
                     /locus_tag="ACG8Z3_001872"
     CDS             5431..6918
                     /locus_tag="ACG8Z3_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0824180.1"
                     /translation="MKKLWKTLFAALLVVPLFTGVFGEKTVSATEATTTNVTINKRIW
                     KDGEAPAQGSMQNTGEEMDFGGDPLEGAGFTAYDVTDAYLALIADGKSQAEATKEIAD
                     NASDYTTVAKAEQKTDANGQTTFEGLALKDGDKDKVYMFVETSTPGNVSVTTKAAPIV
                     LAMPIYTFKDGKYSDTINTNVQVYPKNETKTDKKEVANLDKFTEVKDADGNVLYHNLT
                     TGDEIEYKLTLNIPADILEDGVTYSVTDTPTAGLAYVKDSFAATGLVAGTDYELAEDS
                     ATGGFTVTLKQSENVGKLAGSQLIATYKMKLTAEVNPDDLVNNSAQVTIGNNPQDKIT
                     PPTQFGTGGYKFVKKDSQSGATLSGAEFVVKQGSNFAIFEDAKNTKGEYIFKEWTTDE
                     AKATKIISDDKGELKVIGLKNGDYQLEEKATSSDKYVLLDEDVDFTVEHGQYGSQELK
                     SVLNTPKGLLPSTGGNGIYAFLLIGAALMIGAYAWFKKSKTQAEV"
     gene            7004..8200
                     /locus_tag="ACG8Z3_001873"
     CDS             7004..8200
                     /locus_tag="ACG8Z3_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0824181.1"
                     /translation="MDQRQKNERINLILKLLMAILFTTGAIIFSYPFLSNAVNSYYDQ
                     KMMEKNLAEMAATNTKEQAKRHQEMAEKNKELAESKNMTNIPGMGLVEDPFENAVDDA
                     KDPGKAYYEEHTVGAIYIPKISVSLPLFDETNAALLEKGATILQGTSYPIGGDSTHSV
                     ISGHSGLTERKLFTDLEKLVIGDDFYLTIAGEKLAYAVDDIQIVLPSDIDKVVIQPGR
                     DLVTLLTCTPYGINTHRLLVTGHRVPYVEEMAEEVTSTKKAVERRFRLYLLLIPLFFA
                     MVFYWMYRKFVYYQSGKHSYDFCFYLLENGQPKAGVTFTLVRKKRWATDVTSQPVAVS
                     QVDGWVSFPEIRGGRYYAKAIDGSTKPVKGKVRRLKDRQFVLSRVTKKKHGKKVTYYL
                     ENGAKK"
     gene            8197..9318
                     /locus_tag="ACG8Z3_001874"
     CDS             8197..9318
                     /locus_tag="ACG8Z3_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0824182.1"
                     /translation="MKKNRQVISQIAIILIFMVGILTMLYPFYVDALNNVIDQVRVDR
                     YLKESQKEFEAERKRLAEENSKLSESGLAPGADPFANPNGGTVSAAYYKKHLIGTINL
                     PDLAIELPLFDTTNDDLLEQGATVLDGTSFPVGGASTHAVISAHRGLPERELFTNLPE
                     LKNGDIFLLNVLGETLAYEVFDSQVVTPDQTSVLKIEPGQDLVTLMTCTPYMINSHRL
                     LVTGKRVPYTPAAEKKQVKGDRFRKLKQIAILAGTALLILAAIYQLYHVIARYRLRKV
                     RFDFTVCLEGVAEHTPIALYDKKGKKALRRNGKAYQELTDQTGQVTFTDLPGDCYRLK
                     LGKSWLVQFGLKKKKRPSKIWKINKKKVMLKEERMLEVK"
     gene            complement(9297..9644)
                     /locus_tag="ACG8Z3_001875"
                     /pseudo
     CDS             complement(9297..9644)
                     /locus_tag="ACG8Z3_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10039..11031
                     /locus_tag="ACG8Z3_001876"
     CDS             10039..11031
                     /locus_tag="ACG8Z3_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFQ0824183.1"
                     /translation="MSKDNKDDDLVKNIRKYATTKRLNFLLGSGVSYPAIPLMGSIEG
                     KSEEEKNGKLLEKVKLVSDKLVTNEFCNDTDIYYTLRMYREFILNIIGILHLSNSRQI
                     PRTANIFTTNYDLFIEKATDKVLKNFKFVFNDGASGYFDRKLESTNYNRTVSYKGLND
                     NYTNELPSLTLIKPHGSVNWEKNEEFLYIRNHVIANPCVVKPTGREEEETFLSNHFHE
                     MLRIFQLELDKPQTILFVIGFSFQDKHIAKMLKRALQNPELMTYIFAFDEVDKDKITS
                     NLSHEVGQYNLNILIPKDFSENHKQKNDKGAEFFTLKNLNSILNNVTIGDSKYD"
     gene            11024..12904
                     /locus_tag="ACG8Z3_001877"
     CDS             11024..12904
                     /locus_tag="ACG8Z3_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0824184.1"
                     /translation="MTNPSKKLGVITAVDGDIAQVGMYNMSNDSEFFWYGETLIGPKI
                     GAYLTINQNDNKIIATVINEKIIDQQNSIKSEEFDNRYSKQSINRIISLKTKGVIEND
                     KFQVTSQYVPMIGNEVSLTNMEDLKIIYGINNAKDSIKIGYSILEKQPISLSINKFFA
                     SHIGIFGNTGSGKSNTLHKLYLELFKSEYYEKIVEKSQFFVIDFNGEYISSKTNEKTQ
                     KGIFGVHKDNRTLFKINTRNEDKSSKIPVKKEYLFDADILSILFDARSATQVPFLRSA
                     VREFMKKQNEPFFDFGSYVTGTLKKILTSGLSVNQDSLQDWLDIAKKYLKRDLEFKYL
                     DQLVQFNNGDFKINSLDIWFSKGVETNISDDILTRSGINNISVMLKEYFDNNKDTPLQ
                     QLRIFLDFQKVHRTAWGKINREHLNPLFNRINSSLESLEKVIEIKEDAIGCYKTLNII
                     SLVHANQEITRLVPMLLSKMIYDEQKSKTAGKKVSGTKHLIIDEAHNILNSEYKNNGD
                     SWQDYRLSVFEEIIKEGRKFGFYLTLSSQRPADISPTILSQTHNYLIHRLVNENDLRM
                     LEKTMPTLDKTSYNFIPSLGKGEAIITGSALSIPVLVKIDKETDLRPSSDDINLTSLW
                     ND"
     gene            complement(12933..13538)
                     /locus_tag="ACG8Z3_001878"
     CDS             complement(12933..13538)
                     /locus_tag="ACG8Z3_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0824185.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLENGVAISKISKEYNVTRQTVYRIKNELNT"
     gene            13733..14785
                     /locus_tag="ACG8Z3_001879"
     CDS             13733..14785
                     /locus_tag="ACG8Z3_001879"
                     /inference="COORDINATES: protein motif:HMM:TIGR00341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HFQ0824186.1"
                     /translation="MSENVYYSAKEFYAKINQDLTIRREDMVILVCSIVIACIGLNMD
                     TTPVVIGAMLVSPLMTPIQGIGMGLSLFDQQMLKKALKILLVQIMVSIVTASIYFFIS
                     PISYASNEILARTQPTIWDVIIAFFGGIAGVIGSRKKAMNNIVAGVAIAAALMPPLCT
                     VGYAIALKKMPYFLGAGYLFLINCFFIIIATFIGVQVMRIPKSVKVDSEEKSKMNVVI
                     VILSIVILIPSIISASQLVGKSIIENGLRRMLSEELGNNVIVINKSIDQENKIIYLTT
                     SGEKISDTRIKKIEESLPMYNLKGMSLEISQITNTQQLSGEDLVRLLKEYEESKKSNS
                     IEKTKRVNSTEDSNNE"
     gene            15347..15940
                     /locus_tag="ACG8Z3_001880"
     CDS             15347..15940
                     /locus_tag="ACG8Z3_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0824187.1"
                     /translation="MIFLKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSN
                     RPGLEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSLQENITMDKS
                     SSTGQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLKAQDLELLKTLV
                     DSGTPIKTIAERWNVSRTTIYRYLDKMEGKDGKTNTH"
     gene            15918..16133
                     /locus_tag="ACG8Z3_001881"
     CDS             15918..16133
                     /locus_tag="ACG8Z3_001881"
                     /inference="COORDINATES: protein motif:HMM:NF024908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824188.1"
                     /translation="MEKQTLTSFSEQQRIAAMKKYKIIEPYLNKQKTIKEIAIKNKAP
                     TRTLYRWVQKYEHDGLVGLIRKTRTDF"
     gene            16454..>18337
                     /locus_tag="ACG8Z3_001882"
     CDS             16454..>18337
                     /locus_tag="ACG8Z3_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFQ0824189.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPLFTDETFIT
                     FGFGHLVELAEPGQYNEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVAELLKKANT
                     IIVATDSDREGENIAWSIIHKANAFSKDKTFKRLWINSLEKDVIRSGFQNLQPGMNYY
                     PFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIYQRQEAIE
                     NFKKEPFFEVEASIKVNQGSFKGVLSPTQRFKTQEELLSFVSSNHAKIGNQEGRIADV
                     QTKEKKTNSPSLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITE
                     NEFAYLKANFGKYSGFLGLDLEMVQTEPRKRYVDGSKVQEHHAIIPTKQVPTESALAK
                     MDDLQRKIYALVVKTTVAMFLPDYLYEETKIQTKVADLLFQSIGKTPKQEGWKILFKQ
                     QTKEEKEDVQTLPLVIIGEHAEVDVKSAEKETQPPKAFTEGTLLTAMKTANKTVDDEE
                     AIKILQEVEGIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKLLCQAVESQHLLTS
                     AEMTAKWETYLKKIGKREGNQENFITNIKKFIVHLLEAVPNDIEKLNFSDYQEQKEKE
                     AEKS"
BASE COUNT         6427 a         2961 c         3695 g         5254 t
ORIGIN      
        1 ttaacaattt aatagaacaa gaccatcgac ctattaaacg acgaaataaa ttttatcaaa
       61 gtctccgtac agcttcttcc acgattaagg gcatggagac cattcgagga atatataaaa
      121 agaaccgaag aaatggaacg ctcttcggct tttcggtgtc tactgaaatc aaggtattaa
      181 tgggaataac agcctaagat atttggagtt cagagagggc gcgtttgatt ttcaaacttc
      241 gcaacaaaac cattttagga atttgagtag tgtcatttga taaaaatttc tcaagcagca
      301 ttcttaaatg gattagtgtc aaattattgt tttcgtttca aaaacatcct tgtatagcaa
      361 gatagcttgt tgaacatcat gatgttcaac aaggtcgtaa cgtttttttg agtgttgttg
      421 tcttcggata ttgaattcaa acaatcacta cttatcacta acaagtcaat tatcagattg
      481 aacataaaag tggattgaca gctgaagcag ttgcagagct caaagattac tcaaataaac
      541 aatttcaagg ctagtatatg atacaaaatt catcattagg gtaaaaagaa gtgtttggag
      601 atacggtacc ttatacgata aaagcgacct atccattttt atttctgcta gacaatatct
      661 ctgtgaaaat cggaattgaa gggtaagtgc cttctcaagt gagttgaaga tattggaaaa
      721 gatttttcct tagtgttacg gctcgttttc agtttaatcg tgggtgcgct tcttcttaga
      781 gtagtcattt attatcaaga catcataaaa gttgcatgga aacagtgacg gatacagcaa
      841 agcataggaa tgtgaaagag ataacaatgg tattatcgct ataaattgtt tataatttag
      901 atggcagtat caaatcttag gtagtgctat ttaaattttg tttaagaata gatatcatgt
      961 tttttagagg agggaagaaa tgaagaagaa aaaatcggtc tggtttatga tggttgctat
     1021 tttgatgggc tttatcccgc aaatcttgac tgcttattcg agtatcgcac aggctataga
     1081 aactcagccg gataaaatcg tagatcaatc aggtttaaaa gtcagttcct ctgtttcatc
     1141 agatggaaat tctttaaact gggaacttca atatgaaaaa agtgtagcaa gtgatggcaa
     1201 tgatcaagca ctgaaattta aagtgacggc tgatggagaa gcaatagctt ttaatgaaga
     1261 tgccagtttt gctgttgatg atgaccaatg gtttgcggaa aaagattttt caaaacaatc
     1321 gcagggttct ctttcattca caacggtgct aaatgtctcc aaggtagctc ttgaaattca
     1381 agccgatgca acgaaaacgg atggaacaca agctgtcact attaacaaga atattttgac
     1441 tagtgcggta gaaggtccgc atgcgttgaa attaccagca gtggctacac aaaaaacgac
     1501 agaagtaagt gctacttctc agacaacagc tgaagaaacc actagtagcc aagcgaccga
     1561 aacgacgaca caaacggaag aagccagtga agcaaagtcg tctgagataa atgcctcgcc
     1621 ggcaacagtg ggcgctagca atcttgaata tcttagtgga ataagtgcat tttcttccat
     1681 taattacagc aatattgcgc cagaatatac gacagatgcc acaaaaggaa cttatccgac
     1741 taatagctgg caaccagctg ggcaaacaaa tgtgatcaac caccaaggga acaaggaacg
     1801 caactttgcc acttgggatg gtgtaactag ctggaatggc gatccatcag acacaacaca
     1861 ttcttatatc cagtatggtg aaaaacaaac agattttgcc attcggaagt atgccaaaga
     1921 aacgagtaaa ccagggcttt acgatgtata tttgaatgta aaaggtaata agcaagaaga
     1981 tatcaaacca gttgatattg ttttggtagt ggatatgtca ggaagtatgg aatcgagtca
     2041 aagtaacggc tggaatgatc gtgctggtgc agccagaaat ggtgtgaaaa atttcttgca
     2101 aaccattaaa gacgcaggca ttggggatta cgtcaatgtt ggtttagtcg gattttctag
     2161 tccgggatat gtaacagggc ctaacggtta tcttactgta ccgataggta aagcttcgga
     2221 tactagccac ataaatgcaa tcaatgatgc tttaaaacca aaatttacag gaggtactta
     2281 cactcaaatc gggatcgagc aaggtcagca gatgcttgcg ggaagctcca atgaaaacaa
     2341 aatgatgatc gtattgaccg atggggttcc tactttttct aagaaggtaa ccgctgcgca
     2401 aacgattgat gggactacct atgctactga atttggtaat agattggatg aaccaagcac
     2461 tacatctgga ctcccatggt gggataggta tacggttgat agaacactga ttaatagtac
     2521 ttggccggcc acattaggag cagctaaaat cgccaaagat gcgggcttaa ctatccactt
     2581 gctgggaatt cagctgagta aggatagaaa ttttttaact gaacaacaag ttcgcgatag
     2641 agcaagtctt atcgctacac cgggtaaata caaagatgct gaaacaacag acgatgtttc
     2701 tgattatttg aatgaacaag ctaaaaatgt agtgaaatct ttcaacacca tagtcaatgg
     2761 ttcgatcaat gattctttgg gtgatcagtt ctcttatgaa ggcactccaa ctgtgaagag
     2821 catcagtaca ggggatagtg ctgtaacgaa tttaccgacc gtaaaccagt cgaatggtaa
     2881 agtgaccact actgagttga acttaggcaa agatcaagaa attcagatcc attatcaagt
     2941 gcgtatcaat acagaagata aagatttcac gccggaaaaa tggtatccga tgaatggtca
     3001 aacaactttg acaccaaacg gagacaatcc ggataataaa gtagactttg gcgttccttc
     3061 tgcaaaggca ccgggggtta aactcgatct gaaaaagatt tgggaagaat acgataagga
     3121 tacgagtaaa cgtcccgatt ctcttaattt caccttgaac cgaaaagata ccacagagag
     3181 caattcttgg aagcaaggtt ttattcgtct ttctaaaagc gatgttgcta cagatactaa
     3241 tgtctgggaa aagaccaata ttgaaaaagt agccgaaaca caaggagcta ctgaatcgct
     3301 ttggttgcca caattcaaca acaaagggaa agattttgct tatagctttg aagaagcaac
     3361 ggtcaatggc tatgaatctt caagtaatgc tgctacaacc atttggacca ataaaaaaat
     3421 ctacacacca cttgctttag agatcactaa aatttctgat caaggaaaga cacctcttaa
     3481 aggagcagtc tttaaactaa gtggaggtag tttgccaaat gcaggcgttg aacttaagga
     3541 caacggggat ggaacctata cgttgtctga tgataagtac aagctgcaat taaatactga
     3601 gtatacattg acagaagtta caccaccagc tggacatacg gctactacaa ctagttggaa
     3661 agtcgacgtt agtgcagaag gtaaagtaac catcaacgat gctgtaccgg gtattgaaat
     3721 caaagacaac actatcaagt acaccattga aaatgagttt accaaacgtc ctttattggt
     3781 tgaaaaatat aataaagata gtggtaaaac attagacggt gcgaagttca cgttgaagca
     3841 atatgacgat aagtggacga atgatggtaa agttgtcggt gatgatttga tcggaaatga
     3901 tgccaaatct tttgctagcc tggcaccagg ctattacagc ttggaagaaa cgaaggtacc
     3961 gacaggctat aagaaagacg atacagtctt taagttccaa atcgatagcg ctgggaaact
     4021 acttgatgca aatggcaaag aaatcaccgc taactcaaaa ccaacgacag atggtttcta
     4081 tttaacgagt gataataaga ttgttttaat caaatacaat gaactgcgtg attttgactt
     4141 ttcaattctt aaaaagaact cgcaaagcaa caaaccatta gcagatactg aattcagctt
     4201 agccacaaaa gaggattcca atacgatttt ggctactctg aagaccaatg atgaaggaac
     4261 gggtcagttt ttagatgctt cgggtaatca ttacggtgtt cgtccaggaa cttacgttat
     4321 caaagaaacg aaagcaccag aagggtttgt ccttttaaaa gggattttcg aggtcactat
     4381 aaatgcggac ggaactgtcg gcgacgtgac ttatgatggt aaggatcttg ataaagatgc
     4441 tatcaaagta aatctaaaag atgaggacaa taacgtcatc gaattgacgg ttgctaatac
     4501 acccaaagga caactgccag caaccggcgg atcaggtata cagactttca tcatagtagc
     4561 cttagctctt gtcacagcag caggtgctct tacgatctgc tacttctacc gtaatcgaaa
     4621 ggagttgaac taagatgaag aataaatcat tgttcatcaa aatcaccata cttctttcaa
     4681 ctttgctggt agggatattt acggggcaac aagcctttgc ggatgacggg acgatcgata
     4741 ttgtgatcca caaacaattg tgggaccaag cacctgatgc agctattcaa aatacgggag
     4801 cggccgatca agatatcaaa gatccaatcg aaggggtcgg ctttacgatt tatgacatta
     4861 cagaggatta ttataaattg gtcaaaggct ctgacgaaac aacaactatt caaatgattc
     4921 aagaaaatgt tgctgattat caaaaaatta tcagagaaca aggtctgact gacgaaaaaa
     4981 gcacaatcaa atttacccaa ctacctaaaa gcagtggcgg taaaaatgcg gtttacctga
     5041 ttgttgaaac ggcattgcct gatcttgaaa aggtaaaagt cgaaaaagcg gccgaaccct
     5101 tagtagtggc tttaccagta tatgaaatga gcacagacgg taaaacctat acggacaaag
     5161 aattagctac cgttcacctt tatcctaaaa atgtgggaac aatcgttgaa aagccagtga
     5221 caccaactaa acctaaaaaa ccaagtaaac cgaagaaaca cttgccacaa acaggtgaag
     5281 caaaatcctt cctaggtata ttggggattc ttttgatcgg tacggcgatc attttatggc
     5341 ggaaacactc tgtacgaaac aattaatcaa aggtacaaaa gactaaggtc tattgaaata
     5401 ttgtacaaaa aaacaaggag gaattagaaa atgaagaaat tatggaaaac actttttgca
     5461 gcactacttg tagtaccttt attcaccggc gtgtttggtg agaaaacagt aagtgctact
     5521 gaagcaacaa cgacaaatgt aacgatcaac aaaagaatct ggaaagatgg cgaagctcca
     5581 gcacaaggtt ccatgcaaaa tactggggaa gaaatggact ttggtggaga tccacttgaa
     5641 ggagccggct ttacagccta tgatgtaaca gatgcttatc ttgctctgat tgcagatggt
     5701 aagtcacaag cagaagcaac aaaagaaatt gctgataatg cgtcagatta cacaactgta
     5761 gcaaaagcag aacaaaaaac ggatgccaat ggtcaaacaa cttttgaagg acttgcttta
     5821 aaagacggcg acaaagataa agtctatatg tttgttgaaa caagcacgcc aggcaatgtt
     5881 tccgtaacga caaaggccgc tccaatcgtt ttagcaatgc caatctatac gtttaaagat
     5941 ggcaaatatt cagatacaat caacaccaac gttcaagtct atccaaagaa cgaaaccaag
     6001 actgataaaa aagaagttgc gaatttagat aagttcactg aagttaagga tgctgatgga
     6061 aatgttttat atcataacct tacaactggc gatgaaatcg aatataaatt aactttaaat
     6121 attcctgcgg acattctgga agatggcgtc acttactctg taactgatac gccaactgct
     6181 ggacttgctt atgtaaaaga ttcttttgca gcaactggtt tagtagctgg aactgactat
     6241 gaattagccg aagattctgc aactggcgga tttactgtta ctttgaaaca aagtgaaaat
     6301 gtaggaaaat tagctggtag tcaattgatt gctacttaca aaatgaaatt aactgctgaa
     6361 gtaaatcctg acgatttagt aaacaatagt gcccaagtaa caattggtaa caaccctcaa
     6421 gataaaatta cacctccaac acaatttggt acaggtggtt ataaattcgt caaaaaagat
     6481 tcgcaatctg gtgcaacctt atctggtgcg gaatttgtcg taaaacaagg ctctaacttt
     6541 gcaatatttg aagatgctaa aaacactaaa ggtgaatata tctttaaaga atggacgact
     6601 gatgaagcaa aagcaacgaa aatcatttct gacgacaaag gtgagttgaa agtaattggt
     6661 ttgaaaaatg gtgactatca attagaagaa aaagcgactt caagtgataa atatgtgtta
     6721 cttgatgaag atgtcgattt cactgttgaa cacggtcaat atggtagtca agaactaaag
     6781 tctgttctta acacgcctaa aggtctgctt ccatcaacag gtggtaacgg gatctatgcc
     6841 ttcttactta tcggtgctgc tcttatgatc ggtgcttacg cttggttcaa gaaatctaaa
     6901 acacaagctg aagtctaaga tatagtttta aaatagcaaa aggcagcttc atttcttgaa
     6961 gttgcctttt ctttctttta aatagggaga aggaggtgca agcatggatc agcgccaaaa
     7021 aaatgaacgg atcaatttga ttttaaaact gctaatggcg attttattta cgaccggtgc
     7081 cattattttc agctatccct ttttgtccaa tgcggtgaat tcctattatg accaaaagat
     7141 gatggaaaaa aacttggctg agatggccgc aacaaatacc aaagaacaag caaaaaggca
     7201 ccaagaaatg gccgaaaaaa ataaagagct agctgaaagt aagaacatga ccaatattcc
     7261 tggcatggga ctggtagaag acccgtttga aaatgcggtc gatgatgcca aagatccggg
     7321 taaagcctat tatgaagagc ataccgtggg tgctatctat attccaaaaa tttcggtcag
     7381 cttaccttta tttgatgaaa ccaatgctgc tcttttggaa aaaggcgcga cgattttaca
     7441 aggaacgtct tatccgatcg gtggcgatag tacccattcg gtgatctcag gccacagtgg
     7501 cttgacggaa cgtaaacttt ttacagattt agaaaagttg gtgattggcg atgattttta
     7561 tttgaccatt gctggggaaa aattggctta tgcggtcgat gacatccaga tcgttttacc
     7621 gtcggatatc gacaaagtgg tcatccaacc ggggcgggac ttggtgacct tattgacttg
     7681 tacaccttat ggcatcaata cccatcgttt attagtcact ggacaccgtg tgccttatgt
     7741 ggaggaaatg gcggaggaag tgaccagtac caaaaaagcg gtcgaacggc gttttcgctt
     7801 gtatttacta ctgatcccgc ttttctttgc catggttttc tattggatgt atcgcaagtt
     7861 cgtctattat caaagtggca aacacagtta tgacttttgt ttttacttat tggaaaatgg
     7921 tcaacccaaa gcaggcgtga cttttacctt agtccgtaaa aaacgctggg caacagatgt
     7981 cactagccaa ccagttgctg taagccaggt ggatggatgg gtgtctttcc ctgagatcag
     8041 agggggtcgt tattatgcca aggcgatcga tgggtcgaca aaaccagtta agggcaaggt
     8101 ccgccgttta aaagatcggc agtttgtttt aagccgcgtc acgaagaaaa agcacggtaa
     8161 gaaagttact tattatctgg agaatggagc gaaaaaatga agaaaaatag gcaagtgatc
     8221 tcgcaaattg cgatcatcct catttttatg gtcggcatct tgaccatgct ctatcccttt
     8281 tatgtcgatg cgttgaataa tgtgatcgat caagtgcgcg tcgatcgcta tttgaaggaa
     8341 agccagaaag aatttgaagc cgaacgcaag cgactggcgg aagaaaatag taaattgagc
     8401 gaaagtgggt tagctccggg tgctgatccg tttgcgaatc cgaatggcgg tacggtttca
     8461 gctgcttatt acaaaaaaca tttgattgga accatcaatc ttcctgattt ggccattgag
     8521 ttaccgctgt ttgatacgac caatgatgat ttattggaac aaggtgcgac ggtcctagat
     8581 ggcacgtcct ttcctgttgg tggcgcaagt acccatgcgg tgatctcggc tcatcgtggc
     8641 ttgccagaac gggaactctt caccaatctg ccggagctta aaaacggtga tatttttctg
     8701 ctaaatgtct taggggaaac cttggcttat gaagtgtttg acagccaagt cgtgacgcca
     8761 gatcagactt cggttttaaa gatcgaacca ggccaagact tggtgacgct catgacctgt
     8821 accccttata tgatcaatag tcaccgtttg ttagtcacag gaaaacgcgt gccatataca
     8881 ccggctgccg agaaaaaaca agtcaaaggc gatcgttttc gtaagttgaa acaaatcgct
     8941 atcttggcgg ggacggccct gttgatctta gccgctattt atcaattgta tcatgtgatc
     9001 gcccgttacc gactgcggaa agtgcgtttt gatttcacag tttgcttaga aggcgtggca
     9061 gaacatacgc caatcgcact ctatgataaa aaagggaaaa aagccctgcg gcgtaatgga
     9121 aaagcctatc aagaactaac agatcaaacc ggacaagtca cttttaccga tctaccaggg
     9181 gattgctatc gtttgaaatt gggcaagagt tggttggtgc agtttggttt gaagaaaaaa
     9241 aagaggccat ccaagatttg gaagataaat aagaaaaaag ttatgttaaa agaagaaaga
     9301 atgctagaag tcaaataaaa aatgctcatg ttatcaaatg atttaaatcc ataagataca
     9361 cgtttcattg tttaatatgg gcattcttag cttctatttt gtcattggta tggggataaa
     9421 tcaatgcact tcgtatacct tcgttatatt tgagtaaatt ttgtagtttt ttctaaattc
     9481 ttcgtctaaa ctttctgtaa gaagtgggaa tttaggtgca cggaaactac accaagtttt
     9541 ttaatattcc gtctgttttt agttaagaaa cgccaattta ctttcagttt ctgtgcttca
     9601 tttttgagcc cttttaattg cagctttttc atcattctta cacgaaaggc ttgaaacgga
     9661 tgttccagtg acctcgttta gagcgtacat ttattcaaga aagtttaaat aagatatcaa
     9721 aatggccgaa aaaagacaca caactcgatt tattttttag ggtgaaggag agagtagcac
     9781 gaaaactttt agttggcttg gactgaacga agtgagggaa aggctactaa aacgtcgagg
     9841 ggcagtgaga gcgaagcgaa cacttgatct tttaagttgc tatcatttat aggtcaatag
     9901 agtatactct gttgggtgtt gaactataat tagaatatgt gtattacttt gaggtcatca
     9961 atattaggca taaggggtta ttttataact gtgaacttgt agtataatat aagtaaaata
    10021 tatatttggg agaatacaat gtcaaaggat aacaaagatg atgatttagt aaaaaatatt
    10081 agaaaatatg cgactacgaa aagattgaat tttttgttag gatcaggggt ttcatatcca
    10141 gctattccgt taatgggaag tatagagggt aaaagtgaag aagaaaaaaa tggaaaatta
    10201 ttagaaaaag taaaattagt aagcgataaa ttagttacaa atgaattctg taatgatact
    10261 gatatatatt atacactaag aatgtataga gaatttattt taaatattat tggtatttta
    10321 catctatcaa attctagaca aattcctcga acagccaata tttttacaac aaattatgat
    10381 ttgtttattg aaaaagcaac agataaagtt ctaaaaaact ttaaatttgt gtttaatgat
    10441 ggggcaagtg gatatttcga tagaaaattg gaaagtacta attataatag gacagtatct
    10501 tataaggggt taaatgacaa ctatacaaat gagttacctt ctctaacttt aataaaaccg
    10561 catggttcag taaattggga aaaaaatgaa gagtttctgt atattagaaa tcatgtcata
    10621 gcaaatccct gtgttgtaaa accaactgga agagaagaag aagaaacatt tttaagtaat
    10681 cattttcatg aaatgctgcg tatatttcaa cttgaattag ataaaccaca aactatatta
    10741 tttgtaatag gtttttcttt tcaagataaa cacattgcaa aaatgcttaa acgtgcgttg
    10801 cagaatccag agttaatgac gtatattttt gcatttgatg aggttgataa agataaaata
    10861 acaagcaact tatcgcatga agttggtcaa tataatttga atatattgat ccctaaagat
    10921 ttttcggaaa atcataagca aaaaaatgat aaaggagcgg aatttttcac attaaaaaat
    10981 ctaaattcta tcctaaataa tgtaacgata ggagatagta agtatgacta accccagtaa
    11041 aaagttagga gtaattactg cagtggatgg agatatcgct caagtaggga tgtataatat
    11101 gtctaatgat tctgaatttt tctggtatgg agagacatta ataggtccaa aaattggtgc
    11161 ttatcttacg ataaaccaaa atgataataa aattattgct actgtaatca atgaaaaaat
    11221 aattgatcaa caaaactcaa taaaaagtga agagtttgat aacagatatt caaagcaatc
    11281 aataaacaga attatttctt tgaaaacaaa gggcgtaatc gaaaacgata aatttcaagt
    11341 aaccagtcaa tatgttccca tgattggaaa tgaagtctca ctaacaaata tggaagattt
    11401 aaaaattatt tatggtatta acaatgcaaa agattctatt aaaattggat attctatttt
    11461 agaaaaacaa cctatcagtt tatcaataaa taaatttttt gcttctcata tcggtatatt
    11521 tggtaataca ggaagtggta aatctaatac actacacaaa ttgtacttag aattgtttaa
    11581 atcagagtac tatgaaaaaa ttgtagaaaa atctcaattt tttgtgatag attttaatgg
    11641 tgagtatatt tcttctaaaa caaatgaaaa aactcaaaaa ggtatatttg gagttcacaa
    11701 agataataga acacttttta aaataaatac tagaaacgaa gacaaaagtt caaagattcc
    11761 agtaaaaaaa gagtatctgt ttgatgcaga tattttatca atattatttg atgctcgttc
    11821 tgcaacgcaa gtaccttttt taagaagtgc agttagagaa tttatgaaaa aacaaaacga
    11881 accctttttc gattttggat catatgttac aggaacgcta aaaaaaatat taacatcagg
    11941 actttcggtg aaccaagata gtttacaaga ttggttagat atagcaaaaa agtatttgaa
    12001 gagagattta gagtttaaat atttagatca gttagtccaa tttaataatg gagactttaa
    12061 gattaattca ttagatatct ggttcagtaa aggtgttgaa accaatattt cagatgatat
    12121 attgactcgg tctggaataa ataatatttc tgttatgtta aaggagtact ttgataataa
    12181 taaagatact cctttacaac aattgaggat atttctagat tttcaaaaag tacatcggac
    12241 agcatgggga aaaattaata gagagcattt aaatcctcta tttaatagga ttaattcttc
    12301 tttagaaagt ttagaaaaag taatagagat aaaagaagat gctatagggt gctataaaac
    12361 attaaatatt atttctcttg ttcatgctaa tcaagaaatt actcgtttag taccaatgtt
    12421 attgtctaaa atgatttatg atgaacaaaa atcaaaaact gcaggaaaga aagttagtgg
    12481 aacaaagcat ttaataattg atgaagctca caatatatta aattcggagt ataaaaataa
    12541 tggagatagc tggcaagact atagattatc agtatttgag gaaattataa aagaaggaag
    12601 aaaatttggc ttttatttaa cattatcaag tcaaagacca gctgatattt cgcctacgat
    12661 tctttcgcaa acacataatt atttaattca tcgtttagta aatgaaaatg atctacgcat
    12721 gctagaaaag acaatgccaa cccttgataa aacatcttac aattttattc ctagtttagg
    12781 aaagggagaa gcaattataa caggaagtgc tttaagcatt cctgtattag tgaaaataga
    12841 taaagaaaca gaccttagac ctagtagcga tgatataaat cttacgagct tatggaatga
    12901 ttaaatatca ttagtattca atttatactg tattaagtat ttagttcatt ttttattcta
    12961 tacacagttt gtctcgtaac attatattcc ttagagattt ttgaaatagc aacgccattc
    13021 tcaagatcct caacgatgtt tttataaaca aggcgacgtt ggggatcttt tgcgttcgca
    13081 ctataaagct taggtcgacc tttatacaca ccattagctt tggcaatttt aattccttgt
    13141 gcttgtcgac gtttggattc agtacgttct tgctcggcaa tcatagctaa aatttgaatg
    13201 atcaaatctt taatgaattt atccaggaga ggatttccaa ttgcttcagc catgatcggt
    13261 agactggtga tgatcagctg cacgtttttc ttctttaggt aattgactga agcaatgatt
    13321 tcgtcgtaat ttcttccaag tcggtcgatt gcttcgacaa taaaaatatc ttggtctcgg
    13381 acaaactcta aagcttcctg gaaaatcggt cgatgagtaa tcgttgctcc agattgtttc
    13441 tcgatgaaaa ttttttctgc gccgaacttt ttcatttctt caatttggcg ttgctcattt
    13501 tgatcaatcg acgaaactcg aatataagca attttcaaaa tttttaccta cttcctcaaa
    13561 ctatttaaaa tacgccctaa tctgtcactt ttctatttaa atggcataaa tttttctgat
    13621 tatttggcat acctatttct acttaacgag agattaatta tgatataatt agggacgaat
    13681 aactatgaat ttaatatgaa aataaagaaa cttacagtta aggaagaaaa tgatgagtga
    13741 gaatgtatat tatagtgcca aagaatttta tgctaaaatc aaccaagatt tgactatccg
    13801 cagagaagat atggttattt tagtttgctc aatcgttata gcttgtattg gcttgaatat
    13861 ggatactact ccagttgtta ttggagcaat gcttgtatcc cccctaatga cacctatcca
    13921 aggtatcggg atggggttgt ctctttttga ccaacaaatg ctaaaaaaag cgttaaaaat
    13981 ccttttagta caaataatgg tttctatagt gacagcaagt atttattttt ttatttctcc
    14041 tatttcttat gcaagtaatg aaattcttgc acgaacacaa ccaacaattt gggacgtgat
    14101 tattgctttc tttggaggaa ttgctggggt cataggttct cgaaaaaaag caatgaataa
    14161 tattgttgcc ggtgtcgcaa ttgctgcagc tttaatgcca cctttgtgta cggtaggtta
    14221 tgctattgct ttaaaaaaaa tgccctattt tttaggagca ggatatctat ttttaatcaa
    14281 ttgttttttc attattatcg ctacgttcat tggtgttcaa gtcatgcgta ttcctaaaag
    14341 tgtaaaggtg gatagtgaag agaagagtaa gatgaatgtg gttattgtta tactgagtat
    14401 tgttattctg atcccaagta taatttctgc ctcacaactt gttggaaaaa gtattattga
    14461 gaatggatta agacgaatgc tctcagaaga attgggtaac aacgtcattg tcattaacaa
    14521 atcgattgat caagaaaata aaattattta tttaacaact tcaggtgaga aaatatctga
    14581 tacaagaata aaaaaaattg aagaaagttt accaatgtat aacttaaaag gaatgtcctt
    14641 ggaaataagt cagattacaa acactcaaca actttctgga gaagacttag tcagactttt
    14701 aaaggagtat gaggagtcta aaaagagtaa ctctatagag aaaacaaaaa gagtgaattc
    14761 tacagaagat tcaaataatg agtaaatgga aagtagttag aaaatgaact ctgtatcgga
    14821 gttgagcgcg gttgatatga atttttatat ctgtgaagat tgcgtattgc ctgatagaat
    14881 gaaccaaagt attgagaatc tactgacagg atctattgct tcagattttc aaaattcgat
    14941 agaataagaa attagttagc tagcttaaat ttaataaata gaaagactga ttgtcagtag
    15001 ttcgtcataa aatttataaa acctgaacaa cctttaaaga ggatgttcag gttttattat
    15061 agtgaaccaa accgcttttg tcatagtcac gagtgtttgc aactcatccc gtgcagcaag
    15121 cacccgatta cggttgaggt ctgtaaaacc gtcaatggga aagaagacag ccgttgttgt
    15181 cggagtgatt tttccgtgtt cacaaaatca ggcgtttctg aacagtctat ttttttatct
    15241 tatttctata attttgattc tattatgtgt tcacaaaccc atatcaaaat ctgtgttcat
    15301 ttatactatt ggtataaagt tatgttacaa taaaattaat gaaggagtga tttttttgaa
    15361 aataggatac gcccgggtat ctactggttt acaaaatttg gatttacaag aagacagcct
    15421 aaataaattt ggttgtgaaa aaatttttac cgatcatatg agtggagcaa aaagtaatcg
    15481 accaggatta gaaatggcga ttgattttgt tcgctctggc gatacacttg ttgtttggcg
    15541 cttagatcgt ttaggtagaa atatggaaga tttgatttca atcgtcaatc gactgaatga
    15601 gcgaggtgtt agttttcata gtcttcaaga aaatattacg atggataaat ctagttcgac
    15661 tggtcagctc atgtttcatt tgtttgcagc attcgcagaa tttgaacgca acctgatttt
    15721 agaaagatcc gcagcaggac gagcagcagc tcgagcaaga gggcgatttg gaggacgtcc
    15781 tgaaaaatta aaagcacaag acttagaatt attaaagact ctagttgata gtggaacacc
    15841 tattaaaacg attgccgaac gttggaatgt ttctcgaaca acgatttatc gttatttaga
    15901 taaaatggag ggaaaagatg gaaaaacaaa cactcactag tttttcagaa cagcaaagaa
    15961 tagctgcgat gaaaaaatat aaaattattg agccttatct taataaacaa aaaacgataa
    16021 aagaaatagc tattaaaaat aaagcaccta ctcgtacact ctatcgctgg gtacaaaaat
    16081 atgaacatga tggtctagtt ggtttaatta gaaagactcg aactgatttt tagcaaatta
    16141 gggtttcaga agaagttcga caaaaaatag aagaattggt attgagatac aaaaaaataa
    16201 gtactaagac attatctcga aaaattgttt cctatcatat ttagtggaat tgttgtcttt
    16261 ttatttgtaa gaggaaatct ttctttttcg tttagagata ttttaatgcc tttcataggt
    16321 ttatttgctt tatttggtat ttatgaaagt gcgaatcaat caagaaaaag aaaaataaag
    16381 aaagaaatgg aaagaatgaa agccaaagat catattaagc agtaataaaa aaatctagta
    16441 aggaggcttt cttatgagta cggttatttt agctgaaaaa ccaagccagg cattagccta
    16501 cgcaagtgct ttaaaacaaa gcaccaaaaa agacggttat tttgagatca aagacccact
    16561 atttacagat gaaacgttta tcacctttgg ttttgggcat ttagtcgaat tagcagaacc
    16621 aggtcaatat aacgaaaagt ggcagaactg gaaacttgaa tctttgccga tttttcctga
    16681 tcgatacgat tttgaagttg caaaagataa gggaaagcag tttaaaattg ttgcagaact
    16741 tctcaaaaag gcaaatacaa ttattgttgc aacagatagc gacagagaag gtgaaaatat
    16801 cgcctggtcg attatccata aagcaaatgc cttttcaaaa gataaaacat ttaaaagact
    16861 atggatcaat agcttagaaa aagatgtgat ccgaagcggt tttcaaaatt tgcaaccagg
    16921 aatgaattac tatccctttt atcaagaagc gcaaacacgc caaattgccg attggttgat
    16981 cggcatgaac gcaagccctt tgtatacgtt aaatttacaa caaaaaggcg tacaaggtac
    17041 attttcttta ggacgtgtac aaacaccgac tttatatctc atttatcaac gccaggaagc
    17101 catagagaat tttaaaaaag aacctttttt tgaggtggaa gctagtataa aagtaaacca
    17161 agggtcattt aagggcgttc taagccccac acagcgattt aagacccaag aggaactttt
    17221 atcctttgtt tcttcaaatc acgctaaaat aggcaatcaa gagggcagaa tcgctgacgt
    17281 gcaaaccaaa gagaaaaaaa cgaatagtcc tagtctgttt tctttaagta gtttgcagtc
    17341 aaaagtcaat cagctttata aagcgacagc gagccaaact ttaaaagcta tgcaaggact
    17401 gtatgaagca aaattattga gttatccaag aacagataca ccatttatta cagagaacga
    17461 atttgcttat ttaaaagcga attttggcaa atatagcggt tttttaggac ttgatcttga
    17521 aatggttcaa acagagccta gaaagcgtta tgtggacggt agtaaggtac aggaacacca
    17581 cgccattatc ccaacaaaac aagtacctac cgaatctgca ttagcgaaaa tggacgattt
    17641 acaacgaaaa atttatgctt tagtcgttaa aacgaccgtt gccatgtttt tacctgatta
    17701 cttgtatgaa gaaaccaaga tacaaacgaa agtagctgat ttgctgtttc aatcgatcgg
    17761 aaagacacca aaacaagaag gttggaaaat tcttttcaaa caacaaacca aagaagaaaa
    17821 agaggacgtt caaacgttac ccttggttat cattggcgaa catgccgagg ttgacgttaa
    17881 gagtgccgaa aaagaaacac aaccaccgaa agcttttaca gagggtacat tattaactgc
    17941 tatgaaaacc gctaataaaa cggttgatga tgaagaagca atcaagattt tacaagaagt
    18001 cgaggggatc ggaacagaag cgacaagagc aagtattatt gaagcgttaa aacaaaaaga
    18061 atatatccaa gtgattaaga ataagcttgt tgtaactgaa aaaggaaaat tattgtgcca
    18121 ggcagttgaa agtcagcacc ttttaacgag tgctgaaatg acggctaaat gggaaacgta
    18181 tttaaaaaaa atcggtaaaa gagaaggcaa tcaagagaac tttattacga atatcaaaaa
    18241 attcattgtt catttactgg aagctgtacc taacgatata gaaaaactaa atttttctga
    18301 ttaccaggaa cagaaagaaa aagaagcaga aaaaagt
//
LOCUS       DAZKSU010000053        17963 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000053 DAZKSU010000000
VERSION     DAZKSU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.053"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..815
                     /locus_tag="ACG8Z3_001883"
     CDS             <1..815
                     /locus_tag="ACG8Z3_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0824190.1"
                     /translation="DKKIKKLASTQQLQSRKSKLLFLALVFEDQSYVIIDQSGHPVEY
                     SPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIPVLIEEKRSGKELANCWVGLPE
                     ERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWMLPNEIRKKNTADSLVLTKALR
                     SQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLLGSWQRATKRIREGKPVMIGIL
                     KVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYHKVIPASWSNGTVSLP"
     gene            895..1869
                     /locus_tag="ACG8Z3_001884"
     CDS             895..1869
                     /locus_tag="ACG8Z3_001884"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0824191.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2049..3194
                     /locus_tag="ACG8Z3_001885"
     CDS             2049..3194
                     /locus_tag="ACG8Z3_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0824192.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            3331..3675
                     /locus_tag="ACG8Z3_001886"
     CDS             3331..3675
                     /locus_tag="ACG8Z3_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0824193.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            3946..5232
                     /gene="efmA"
                     /locus_tag="ACG8Z3_001887"
     CDS             3946..5232
                     /gene="efmA"
                     /locus_tag="ACG8Z3_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0824194.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(5370..5969)
                     /locus_tag="ACG8Z3_001888"
     CDS             complement(5370..5969)
                     /locus_tag="ACG8Z3_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0824195.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            6373..7494
                     /gene="mnmA"
                     /locus_tag="ACG8Z3_001889"
     CDS             6373..7494
                     /gene="mnmA"
                     /locus_tag="ACG8Z3_001889"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0824196.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            7740..9161
                     /gene="arcD"
                     /locus_tag="ACG8Z3_001890"
     CDS             7740..9161
                     /gene="arcD"
                     /locus_tag="ACG8Z3_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0824197.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            9175..10293
                     /locus_tag="ACG8Z3_001891"
     CDS             9175..10293
                     /locus_tag="ACG8Z3_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0824198.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(10353..11645)
                     /locus_tag="ACG8Z3_001892"
     CDS             complement(10353..11645)
                     /locus_tag="ACG8Z3_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0824199.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            11795..12355
                     /locus_tag="ACG8Z3_001893"
     CDS             11795..12355
                     /locus_tag="ACG8Z3_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0824200.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            12484..12861
                     /locus_tag="ACG8Z3_001894"
     CDS             12484..12861
                     /locus_tag="ACG8Z3_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0824201.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            12794..14866
                     /locus_tag="ACG8Z3_001895"
     CDS             12794..14866
                     /locus_tag="ACG8Z3_001895"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0824202.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(15077..16024)
                     /locus_tag="ACG8Z3_001896"
     CDS             complement(15077..16024)
                     /locus_tag="ACG8Z3_001896"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0824203.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            16270..16734
                     /locus_tag="ACG8Z3_001897"
     CDS             16270..16734
                     /locus_tag="ACG8Z3_001897"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0824204.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVFPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(16796..17839)
                     /locus_tag="ACG8Z3_001898"
     CDS             complement(16796..17839)
                     /locus_tag="ACG8Z3_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0824205.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         5880 a         3257 c         3815 g         5011 t
ORIGIN      
        1 tagataaaaa aattaaaaaa ttagccagca ctcagcagct gcagtcaaga aaaagtaaat
       61 tgcttttttt agctctggtt tttgaagatc aaagttatgt gattatcgat caatcaggac
      121 atccggtaga atattctcca gcaaaatata cttatcaaga gggtctttct tgtaaaaaat
      181 ggaagctgat caatgaagag ccaatcgagt tatctagatg gatcaacaga aaagaagata
      241 ttcccgtttt gatcgaagaa aaaaggtcag gaaaggaact cgcaaattgc tgggtgggat
      301 tgccagaaga acgttttttg aggtataaga aatgggctac cccctcaggc tatctttgtg
      361 gtacttacgc ggcagcagtt ctccttgcct attatcaaga ctatcgtaag gggtggatgc
      421 ttccaaatga gattcgcaag aaaaatacgg ctgattcgtt ggttttgacg aaggcattgc
      481 gtagccagat ccaaccacta ggacttccta cgattccttt tcaggttagt acagggattt
      541 ctagtttttt aaagaagaat ggcaatcatg agcgtgcacg agcaactctt ctaggttcat
      601 ggcagcgagc tacaaaacgt atacgtgaag gcaaacctgt gatgattggt attttgaaag
      661 tacttggcag tacatatggc aatcattggg tgacagcgta cgcttatttt gaaactgaaa
      721 caggagaacg ttattacaaa gtacacgaca actggggcga ttatcataaa gtcattcctg
      781 ccagttggag taacggaaca gtttcacttc cttaagaatt tgctgaaatt aacagaaaat
      841 cttggaaaaa tatgttaaac tatcgaagaa tagagaagtt ttggggagga ctacatgagt
      901 aataagtatc aggatgacac gttgaagatt tttagtttga atggcaaccg tccattagct
      961 gaaaaaatcg cgaaagtgtt cggaacagaa ttaggtaaaa gcgtcgtgaa gcaatttagt
     1021 gatggcgaga tttccatcaa tatcgaagaa agtattcgag gggatcatgt gtatatcgtt
     1081 caatcaacga atgcacctgt caatgattac tatatggaac tactgatcat gatcgatgca
     1141 atgaagcgag caagtgcgaa aacgatcaat gtcgtattgc cttactatgg gtatgctagg
     1201 caagaccgca cggcgaaacc tcatgaaccg atcactgcga aattgatcgc taacttgatc
     1261 gaagaagcag gggcaactcg agtactgact ttagatctgc acacggttca agtacaagga
     1321 ttctttgata ttcctgtaga taatttattt acgatgccgt tgtttgctca ttattaccgt
     1381 caattaggtc tgacgggaga tgacatcgta gttgtttcgc caaaaaacag cggtgttcag
     1441 cgtgcacgta gtctttcaga atatttgaac agcacattag ccatcgtaga tcatgcagat
     1501 gatgcttcaa ctgagggtgg ttatgtgatc ggtgatgttc aaggaaagac atgtatcatg
     1561 gtagatgata tcttgaacac aggagcaacg cttgcacgag cagcaaatgt attaaaagaa
     1621 aacggcgcaa aagaagtata tgcctgtgcg tcacatggat tattgtccga tcatgcgaaa
     1681 agtattttag acaatgcacc aatcaaagat atttgtatca ctgattctgt tttcacagaa
     1741 gaaaatcgtc atccagaaaa cttaacaatc gttacatgtt cagaattaat gggtgaagcg
     1801 gtcaaacgca tccatgaaaa tacaccgatg agtccgttat tccgtctgga agaaaaagaa
     1861 ttcgaataaa caaaaaaaag agggaaagaa gttgtgacag aagtgagcag cttcaaggag
     1921 ctgcgacaag acttttgtca cagctccttt tttctatgcc catgaaacgt cctctagaca
     1981 aaaatgagct cttctttggt ttgttcgcta ttttatgcta gaatgtgcct agcaataatt
     2041 tggaagggat gaatgaagtg aaaactattt atctggatca tgcggcaact acccccatgc
     2101 atccacaagt aattgaagcg atgacggcaa tcatgcaaga cacgtttgga aatccttcaa
     2161 gcatacatgg atttggtcgc cacgcgcatg aaaaattgga aacagcaaga caaatcattg
     2221 cagacagtct gcaagcaaaa cctcacgaga tcatctttaa cagtggggga acagaagggg
     2281 ataatacagc tatcttagaa acagcgttct cgcgtcaaaa agaaggcaga catctaatca
     2341 ctacagctat cgaacatcct gctgttctta ataccatgaa atatttagag acaaaggggt
     2401 ttgacgttac atatctgcct gtagatgaaa aagggaatct gtcgatcgaa gaggtcaaaa
     2461 aagcgttacg agaagatacg atcttggtct ctgtgatgta cgggaacaat gaaatcggaa
     2521 atttgatgcc aatcaaagaa atcggtgaac ttttagcgga tcatccagct tatttccata
     2581 cagatgcggt acaagcgtac ggaaaccaaa cgatccatcc agaagaatta ggaatcgatt
     2641 tattaagtat ctctgctcat aaaatcaatg ggccaaaagg cgtcggtttc ttatataaac
     2701 gagacggagt aaatatccct gcattgcttt taggcgggga gcaggaagaa aagcggcgtg
     2761 caggtacaga aaatttagca gggatctggg gtatggcaaa agcagttgaa ctgctgtcac
     2821 ctgaagaacg tgaagcacgt aacaacaaat ataaaggatt ccagcaattg attcttgata
     2881 cattagaagc aaacgaaatc ccttatcaag taaatggtga tttaacgaat aaactccctc
     2941 atgtgttgaa tcttcgctta attggcgaag caaatgattt gctactgatg aagcttgatc
     3001 ttaatggggt cgctatttct actggttcag cttgtacagc aggaaatatc gagccttcac
     3061 acgtattaga agcaatgtat ggagaagata caccgatact aaaagaatcc atccgtatca
     3121 gctttggttt gggaaatacc gaagacgaca tccagtattt ccttgatcaa ttagttcgtg
     3181 ctgtcaaaaa ataactagtg aagtcattct tatatgaaca gatgtctgag agaacgcctt
     3241 cattttttag ggaacttaac tgaaaaacct agtaaaaatc aggataaact tttataataa
     3301 agagaaacta attttatcga ggtgaaaatc atggcatttg aaacaacagc aacggtactg
     3361 ggcgcaccaa ttagctatcg tttgcatcct gatgcaaaaa gatatacatt gcgcgataat
     3421 ggatttacag aaacaaacgg aggcaattat caattgatcc gaccattaga tgccacgccg
     3481 caaagcaaag aaggattcaa attaaaaatc actgtatcaa aagatattca aacattgaaa
     3541 atgtcgatca ctacagcaaa tggattgaaa gcagtcaata ttttcaaaga tccaaaacag
     3601 aaaatgagcc aagataaatt ttattttctg atggatggga tgattagccg tgggttattc
     3661 gaaaaagtcg aatgatctgt ggatataaaa agacaactag ttttgaaacg gcctattgtt
     3721 agatgactaa ctttgtaagg acgacgcaaa ttagttgtct tttttatttt taaatttaac
     3781 acatgttgtt atttttaata ttttgctgat ttattgtttc taagtaaaga aatttaaatt
     3841 gaaacgattg ttatttaggg aaaaaggtat tttttgaaaa aagaatcccg tataatgatt
     3901 gttgtgcaaa cactatacaa aatgaaaaag aaaagaggga tgtttatgga aaatgaacag
     3961 tcggtcgttt taacgaattg gaagagaaat tatttgttct ttttatcggg acagtttttg
     4021 tcaggaatca ctagtatggt cgtccaatat gcgatcatct ggtatttgac aagagaaacc
     4081 ggttcggcga caattttgag ttttgctact ttactaggaa tgattcccat ggttttacta
     4141 agcccatttg taggaccgct agtggatcgc tgggataaaa aagctctttt gattgtcacg
     4201 gatattattg tagcgatttt cgcgcttatt ttagcagtag tcggaacgat ttcagaatcc
     4261 ttcccgattt ggctagtttt tgtgtctttg tttatgcgtt cggtcgcaca aacattccaa
     4321 atgccaacga tccagtcgat tatgccgaca atcgttccat cttctcacat aacaaggacg
     4381 aatggacagt taggaatggt ccaatcagcg aatttcatca ttgcgccagc tctaggtgcg
     4441 gctttgtttt cagtcgttcc tgtcaattac cttattttat tagatgtatt gggggcagtt
     4501 tttggtgtcg gcttattgat ttttgtgaag atcccaaaag tttctcctga aatattggaa
     4561 gttccgctta ctatttttaa agatgcaaaa tttgggctgc agcaattgat ggataataaa
     4621 gggttatggt acataacgat caatggtgca tttgtgatgc tgttgttcat gccagcaatc
     4681 agtctgtatc cattgatgac gctagattac tttggtggtt ccgttggaca ggcgggagca
     4741 gtggaagtag tctacgcagt cggaatgctc ttaggcggtg cactgatcag ttttatagga
     4801 acatggaaag accgaatgaa acctattatt atagcctaca tcattatggg cctgacgatc
     4861 ggtgcaagcg gattggttcc aaacgatagt caagggttct tgtactttct tatcttgaat
     4921 gcaggagcag gttgtgcaac gccatatttc aatacactgc tgatggcaat gatccagcag
     4981 agttatgaat cgaatgtttt aggtcgtgtc ttagggaact tcaactcttt gatgaacctg
     5041 gcaggtccaa ttggattatt atttgcagga ccattagctg atcgtttagg ggtagaaaaa
     5101 atgttcttgt tttccggaat cgggatttta ttatgcggaa tcgttttatt tttaacgtca
     5161 gctgcccgaa attatgataa agaattacag aaaaaactgg taaaagaaca ccatgagcaa
     5221 aaagacgaat agtatggaat ggagttgtga cagaagtctt gttacaactc catttctgat
     5281 cctaatgttc ctgtggcaat aagcctactc aaatgaaaaa tatgagtaat tatctttatt
     5341 tgtttaatac ttgatatagg gcagtttttt cacgacctat cgtacttcct ctttgcttca
     5401 tagatttctt tgatttgttg ctcagataac ggagtgccaa atggtagttt ttcctttaag
     5461 agttctcttt ccggtgcgtc tacgcctaca ttgacatcgt tctcgatagg tacgctgtaa
     5521 tgatggatat gtccatgaag attgcggata gtgtcgttag gcccgagcaa catcggataa
     5581 tgagtcaagt aatattgttg atggtcgaat ttaacgatgg cacctacatc ataaaaataa
     5641 aatttgggac ttccgtcagt aagcctagga tcattttttt ctaaataacg gaaaaaagcg
     5701 cgactgtcat ggttcccttt gatcaagtgg attgttcctt gcagttgagt gaccagttcc
     5761 aaaatttctg catttgcttt ttcatattgc ggatgcatag caaggtcgcc tagatgataa
     5821 acatggtcgg tttcttttat tactgcattc cagctagtga tcaactgttg gttcatttca
     5881 tccacatttg caaagagccg cggagcaaag tcgttttttc ctaataagcg ttcatggaaa
     5941 aagtgcatat cactgataaa gtaattcatg tatcttttgc ctcccttttt tcttagagta
     6001 aacaaaatca cttatctgag caactcataa catctaaaaa gaaaaggttg taaatgagac
     6061 gtgcagcttt aagcgataag aagcaatttt gaaaaatagt ttttcacaat ttataaaatt
     6121 attccttatt gccgaaaggc tggccctttt tatgagtatg ccaatttttt atatcgaatg
     6181 ctcaaaagat gagtgatttt ttggttgact atcatgtttg tgtactgaat aggtccagta
     6241 gaacagtatt tttacgactt gataattaag ggatgaaatt ttcagaaata tgacgtataa
     6301 tgtaaaatac tgaaaagttg cgaccttcaa ctcgtagatg tttcagaatc taattgaaat
     6361 gatggtgata tcatgacaga caacagcaaa acacgcgttg tcgtagggat gagcggcggt
     6421 gtcgactcat ctgtaacagc acttttgtta aaggaacaag gatacgacgt gatcggaata
     6481 ttcatgaaaa attgggatga cacggacgaa aacggtgtat gcacggccac ggaagattat
     6541 aaggatgtag cgaaagtagc tgctcaaatc gggattcctt attactctgt gaattttgaa
     6601 aaagaatatt gggatcgtgt atttgaatat ttcctagccg aataccgcgc aggacgtaca
     6661 cctaacccag acgtgatgtg caacaaagaa atcaaattca aagcattctt ggattatgca
     6721 atggatcttg gcgccgatta tgtagctact ggacattatg cgcaagtcac tcgcgatgaa
     6781 aacggcgtgg tccacatgct tcgtggagta gacaataata aagatcaaac ttacttcctt
     6841 agccaattat cacaagaaca gctttcaaaa acgatgttcc ctctaggcgg catggaaaaa
     6901 tcagaagtac gggcaattgc tgaacgcgca ggccttgcga ctgctaagaa aaaagattca
     6961 acgggaatct gctttattgg tgaaaagaac ttcaaacaat ttttgagcaa ctatctgcca
     7021 gcaaaaaaag gaaatatggt cactcttgat ggtgaagtca aaggacaaca tgctggcttg
     7081 atatattata cgattggtca acgtcaagga ctagggatcg gtggcggagg agattcgcaa
     7141 gaaccctggt ttgttgtagg aaaagatcta gcgacaaaca cattatatgt ggggcaaggg
     7201 ttccatcatc ctgcattgta tgccacaagc ttggatgcaa gtgaaatcca ctttacgacg
     7261 aatgaaccaa tgccaaaaga attcaagtgt acagcgaaat tccgttatcg tcagcaagac
     7321 gttcctgtga cagttcgctt attggatgat aatcgtgcag aagtcgtatt cgacgaacct
     7381 gtacgtgcaa tcactcctgg acaagcagtt gtattttatg atggaatgga atgtctgggt
     7441 ggtggtttga tcgaccacgc atatcaagaa acaaaagttt tacaatacgt ttaagcgata
     7501 ttaaatgaat caaaacagct atggctaaca aaagaaacac cttttctttt ggatagcata
     7561 gctgtttttt tgcaataaaa ttaattttta ttcattttat acttgaaata cttgtgtaaa
     7621 aagttcataa tatccaataa tatgaataaa gttgcttttt gttaagcaag agcagttgca
     7681 aaatagaagg gaatcttttg caagtgattt gttcaatttt ataagtgagg gtgatggaaa
     7741 tggaaaagaa atcgacgggt attagcaaat ggggactggt tgctctggtt gtcagttcgt
     7801 caatcggaag cggtgtgttt gggatcacaa gtgatctggc tagttctgca gctccagggc
     7861 cagcgatcct ttcatggata atcgttggga ttggtatttt agctttggta ttatcgctga
     7921 atcatttagg agaaaagcgt ccagatctag atggtggtat tttcggttac gcggaagctt
     7981 catttggtaa attaggtggc ttcatcagtg gttggggtta ctggttgtct gcgtggctgg
     8041 gaaatgttgc ttttgcaacg atgctgatga gcgccatggg agaatttttc ccgatattca
     8101 aaggtggaca aaatgttcct tctattttat ttgcaagtat ttttatttgg ggtctgactt
     8161 tattagtaaa caatggagtt gaaggagcga gcgtcatcaa tacaatcgtg acgatctgta
     8221 aattagtgcc attattccta tttttaatat ttgtatttat cgcatttaaa gtgaatatat
     8281 tcactgctga tttttggggt aacgtatctg ataatctagt gaatggaaca gggcagcaag
     8341 gaacgatctg gttgcagatt aaaggttgtt tgatggttat gatgtgggtc tttgtaggga
     8401 tcgaaggcgc aagtgtttta gccaaccgcg cgaagaaacg ttcagaagcc cagcaagcat
     8461 cgatcattgg tctgctttgt ttattgttta tctatatcct tgcttcgatc ttgccatacg
     8521 gcgtcttatc tcaagaagag ttagcaacta tttcacaacc tgctatggca aatattttaa
     8581 aaggaatcgt gggaaattgg ggagcagcgc tgatcaatat cggcttgatt atttcgatca
     8641 ttggttcttg gttgtcttgg acgatgctcc cagcagaaac aacgatgttg atggctcgag
     8701 acggtctgtt gccgaaaggt tggggaaaaa cgaataaaaa aaatgcccca acttattcgt
     8761 tagttgttac tgcaatcttg actaatctat tcttattgac tttcttagtt actgattatg
     8821 cctatcaatt tgcctattct ttatgtacag cggcaatttt gatttgttat ctacttgtcg
     8881 ggatctatca agtccagttt tctttaaaag aaggaatcaa aaaacaagta gtgatcggtg
     8941 ggattgctgt agtttttgaa ttgatgggca tcattttagc aggattttct tatgtccttt
     9001 tatgtagtat tgcctatctt cctggttttt gcttctactg gatcgcttgt cgtgaaacca
     9061 aacatcaaat tacgggaaaa gaaaaagtaa tgattgggct gatttgtgtg gccgcactgc
     9121 tttcaatcgg gttgcttgct gtaggctgga tcaagatata agggggaaag aaacatgaaa
     9181 atcgcaaatt tcggtgtaga agaatggcta aataaatggg aaaagaaagc ggtatatgat
     9241 attgcacaaa gctcgatcga agcattgaca ttagacgaac tcgttggctt agatgggact
     9301 agcgtaagta aatttttcga aaaacgcaaa aacgaaccat tggactatgg ctggatcgaa
     9361 ggatcagatg ttttcaaaca agaagtcgct tctctttatc aaaccgtcga tccggaaaac
     9421 atcttacaaa cgaatggagc gactggtgct aatcttttag ctttatacgc gttagtggaa
     9481 gcaggagacc atgttgtttc gatgcttcct acttatcagc aattatatga catccccaaa
     9541 tctttaggtg ccgctgttga ctttgttcat ttgaaggaag aagaagattg gcagtttgat
     9601 ttagaacagc tagaagcctt agtcaaacct gaaacaaaaa tcatctgttt gaatagtgca
     9661 aacaatccta caggcacatt gctagatcgg aaaactttag aaaaaatcgc agaaattgct
     9721 cgtacagtag atgcatatgt attgatagat gaagtatatg cgcctttaac ggataaaggt
     9781 gaattcttgt cgatcgtaga tgtctatgaa aaaggtatag caaccaattc cctatccaaa
     9841 acctactcca ttccaggtat tcgtattggc tggacagcta ctggacctga gttggcagaa
     9901 gtttttcgaa agtatcgaga ctacacgatg atttgcggcg gagtcctgtc agatgatctt
     9961 gctgtacatg cattgaagaa taaagaaaaa atattggcgc gaaacaaaaa gatcattaca
    10021 gagaaccttg tgattttaaa acaatgggta gccaatgaac cgaaagttga acttgtcgct
    10081 cccaattacg tctctacttc tttcattaaa ttagccatcg aagaagatga ccagacattt
    10141 tgtatcaatt tattagaaga aacaggagta ctattagtac caggaagtgc ctttgattta
    10201 ccgaagcatg cccgactagg ttactgttgt aaaaaagaaa ccttggaaaa gggattattc
    10261 ctcttatctg agttcttgaa aaaacaggcc taaacaaaaa cactgcaagg acagtctgtg
    10321 acaactgttc aagcagtgtt tttcatttac gcttattgtc tatgagaagg agagtgccct
    10381 gaagctatgg agactcctga agctttttct tctgcagttt cttgctgatg gatctcttgt
    10441 gtattgtgtc gtacaagcaa gaagttgacc aaaacaagaa gagaagtcga aagaaagacg
    10501 ccacgataac caaatgcact ggaaacactg gagccgatca taggccctac tacgttacca
    10561 gttgcttgga aagattggtt ataactgaag atccgtccag cagcttgatg aggcgaatat
    10621 tttgtgatca atgcttggac agcagggagc agacaagcat cagagatacc aatcaaaaaa
    10681 cgtaaggcag ctaattgcca aacattcgtg acgaatgcca ttgggatata aaccagaatc
    10741 gcaaaaccca aaccaatcgc aagtattctt tcactcccaa tcttgtcacc taagcgacca
    10801 aatcttggag cggctatcaa agtggcaatc ccaggtatcg aagcaatgat tccgctgatc
    10861 aacgtaacat tcccatgtcc atgcagcaat tggcgaatgt acagactgat gattggacta
    10921 atggaattgt tggatgcttg gatgatcatc gttgtgacga acataccgat gacgacgtgt
    10981 ggatatttca agtctttgaa aatctgtttg gcagaaacca tgttttcttt tttcactggg
    11041 acaaattttt catggacgaa aaacagactc aacaagaaga cgaaaaataa gatgatccct
    11101 gtaatgaaaa aggtagggcg atagccaaac gctgaagcag agatcccgcc aagaagcggt
    11161 ccaagtaatg ttcctgtaac agaaccagtt gctaaggttc caagaacttg accacttttt
    11221 tcttttggcg tcccagttgc gacaagggca gtggcattac tgatataacc agaaaaaatc
    11281 ccttgaagca gacgcaatgc gactaattga tacacactag tcacgcaacc catcaaagaa
    11341 atgacgattg ccattcctaa tgaagcacgc aacagcatca attttcttcc tttttggtcg
    11401 gctaattttc cccaccaagg agagataagc gtagtcacca agaaagtaga tgaaaaagtc
    11461 aatccgctcc aaaagttcaa ttgtgacgta ctataattac ctaacgtatc tatgtaaagt
    11521 gacataaaag gcatgactaa actaaatccg ataccagcca taaatgttcc aaaccataag
    11581 acaaacaagt tttgttccca agtttctctt ttcctaaata atttttcctt tattcttgca
    11641 gccaattaga ctccttcttt ctgaaaattt tcatctacag ctctttatta taaactacaa
    11701 tatgtagttt atcaatgaaa gtctcttttt tctctttctt tttcgtggta aactgaaaga
    11761 gtgaaggatt tattttatga gaggaaacat ggaaatgaaa aaaagaaatc tgtctcaaga
    11821 gaaaatcatc gactgctttc gagaactagc agaagagatg gatgttcagc aaataacctt
    11881 tcagcattta gccaaacgct tggatatcaa ggctccctcg ctgtataacc atttcaaaaa
    11941 tattcgtgat gtcaaaacag cgctaacagc gaaacttctg caggaactta atgaccagct
    12001 gcgacgggct cttgtgggaa aaagtggtca ggaagcactg cagatctatg cagaaactta
    12061 tcgatccttt gctttttcaa atcaagcagt gtatgagtta ctgatcagtg taccgcatac
    12121 caatgaagct gttttattag atggaatcca tgaaacgaat cagatcattt tgcagatatt
    12181 tgaggctttt caattaacga aagaagaaaa agttcatcga agcagggaac ttcgtagtat
    12241 gatccacggt tatctttcct tacgtttttt aggatacttt acaaaagaac caacaatcga
    12301 tccagaagac agctattttt ggatgatcaa taactttatc gctactttgc cttaaacaat
    12361 cagattcatt tgtaacaatt ttaagatgtt cattaaatga cccttttata agagaaaatt
    12421 cttgcaactt tcttgaaaag tgactaagat gagtctagaa aagctgaaag aaggagcacg
    12481 agtatgtatc aagtaattac catgttcggg gacaatgagc cttggtggtt ttttgaagac
    12541 tgggaagaag atattgaaga agaagaaaca ttcgagtctt tcgaagaagc acgacaagcg
    12601 tacgaaaaac aatggaacga aatccatcaa aattatgaat atatcaatgc aaaaagtaac
    12661 tttctcagcg ccttttggaa tgacggagat gagcgctggt gtgaagagtg tgacgatgat
    12721 ttacagcaat acaagggact tgcgttgttg aaaaatcatc agcccatcac agtagaaagc
    12781 agaaaggaat tttatgaaac aactaattct agcggaaaaa ccaagcgttg cgaaagacct
    12841 aagcaaggtg cttggtgcta atcaaaagca caaaaattat tatgaaggac ccaaggtgat
    12901 cgttacttgg gcccttggtc atttattagg attgaaaatg ccggaagatc tgaataagga
    12961 atggcagagc tggcaaatgg acacgttgcc aatgatccct aaaaaactag gaatcaaacc
    13021 acttcctaaa acaggacatc aattaaaagc aatcaagcag ctggctcagc gaaaagatgt
    13081 ctcagaagca gtcattgcga cagatgccgg aagagaaggg gaacttgttg ctcgctggat
    13141 cttagagtat gtgcatttca ataaaccagt gaagcggtta tggatctctt ctcaaacaga
    13201 taaagcaatc aaaactggat tcaagcaact aagacctgcg aaagaatatg atgcattgta
    13261 tgaatctgct cttgcccggg caaaagcgga ctggctggta ggattgaatg tgaccagagc
    13321 cttaactgtg aagtacgcgg ataatttatc agcaggccgt gtgcagacac cgacactagc
    13381 attagtgaga gaccaagaaa agaaaatcga gcagttccgt ccacaaacgt atttcacgat
    13441 cacgttgcaa gctggaaaag aacaagccaa aatgcttcaa aaaaatcaat ttgctttaaa
    13501 aacacaagaa gaagcacaaa aacttgttca attgatcagt acaagcaaag gaactgtcac
    13561 agaagtccaa gaaaaattaa aaacggaatc tgcgccattg ccttatgatt taacagagat
    13621 ccaaagagaa gccaatcaac gctatggcta ttctgcaaag aaaacattaa gtcttgtaca
    13681 aagtttgtat gagacccata agatcgttac ttatccgcgt acagacagca aatatctgac
    13741 aaatgacatg aaaagtacga tgaaagaacg attgcaagca gtcagcgact ttgctcctga
    13801 ggtcaagcag tatttgaaaa acggagccat cgtccaacag acaaaagtat tccaagacag
    13861 taaagtgacc gatcaccatg cacttttgcc aactgaaaac agagcacgtt atgaaaaatt
    13921 aagtaatgaa gagcgaaaaa tctatcaaat gattgtctct cgttttttag gactgtttgc
    13981 tcaaccccat aaagtcagcc aaacaaaagt tacggttgaa tttggtaaag aacgattcat
    14041 ttttcggcaa aatcgtgtcg tccaagcagg atggaaaggc gaaactgaaa ctgagacgga
    14101 aacagtcaaa tgggaaaaag gaatgagaat caatcccgat ttcgccatca aaaaagaact
    14161 atctgcaccg cctaaaccat tgtcagaagc aagcttgctt ggtcaaatgg aaaaatacag
    14221 tctgggaacc ccagctacgc gtgcagagat tatcgagaaa ctaatcaaat ctgaactgat
    14281 ggaacgtacc cctcaaggac tgcaagtatc tcctaaaggt aagcaattat tagtattggt
    14341 aaatccctct ttagtaacac cagatttgac agaaagctgg gaaaaagacc ttgaagctat
    14401 tgctgcagga aagaaacaag caggaacctt cttgaatgga atcgaaaaag aaacaaaacg
    14461 cttggtgaat gagatcaaat caagcaaaca agaatatcaa gatttctcga ttacccaaaa
    14521 gaaatgccca gaatgtgggg ccaatctgcg cgagaaaaac acacgtgacg gcaaaatcta
    14581 tgtctgcacc aattcagatt gcaactaccg cagaagaaaa gatccaaaag tctctaatca
    14641 tcgatgcccg caatgtcacc ggaaaatgga aatcatcgaa ggtaaaaacg gtgcttattt
    14701 ccgttgtaaa tacgatggaa cgacagaaaa attgcttgat aaaaaagagc agaagaagaa
    14761 aatgaccaaa catgaagaac gacgcttgct gaaaaaatat gcacaagcag aagaaccaga
    14821 agaaagccca ttagcagcag cactaaaagc agcaatgaag aagtaataag atatgaaaga
    14881 ggttgtgata gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc
    14941 aaatttttgt gaaatttcgg cttatttccg aagaagctac ttctggaaca ccatttattc
    15001 ggaaaaaagg actgtgacaa gacttttgtc acagtccttt ttcatttaaa aaaatgttta
    15061 cttcatttga cgtatattat tggtgaggct tcaattcatt tggcttcact ttttctgttt
    15121 ctcttatgat ataaacagga cgctgtttcg tttctaagaa gattttccca atatatttcc
    15181 cgatgattcc aagacataac aattggattc caccaatgaa caggaagatc gagaccatgg
    15241 aaggccatcc tgatgtagga tcaccaaaca acaatgcccg caaaataatg aacaaaattg
    15301 caataccaga accgacacaa gacaaagcgc cgataaatga agcaatcgtt aacggtgcat
    15361 ccgagaagtt cacgatacca tcaatcgagt agttgaataa tttccagaag gaccaagatg
    15421 tttcgccagc cacacgttcg cggttttcat aagaaatata ttctgtttta aatccgaccc
    15481 agctaaataa tccttttgaa aatcggttgt attctgttaa ttctaggacg gcatccacca
    15541 tttgacgagt catcaagcgg aaatcacgag cgccatcgac catttctgta tcgctgattt
    15601 tattgatcaa ctggtagaat tttttcgcaa agaaactgcg tatcgcaggc tcgccttcac
    15661 gagtcgaacg ccgggtgccg acacaatcga tatcagaagt agtgatccgg ttgatcatct
    15721 caggaagcag ttcgggcgga tcttgcagat caacatccat cacggtcact aaatctcctg
    15781 tagaagcttg caatccagcg tacagagccg cttctttccc aaaatttctg gaaaatgaga
    15841 caaagcggac ataaggataa gctgaggcta gcttcttgat tgctgctaac gtaccgtctt
    15901 ttgatccgtc gtcaataaac aaatattcga actcatgggc acctttgaat ttttcaacct
    15961 catcaaaaaa aagaggaaca gttttttctt cattaaaaca tgggacaaca attgatactt
    16021 tcatcgtttc tatcctttca atcgtatgtt tatatatggt ttacattcaa aatttaataa
    16081 accttaaata acacctttaa aatttgattt tacgtaaaag aacaggtatt ctttttgtaa
    16141 actaacgtat ttagtttacc atgtttcctc ggtttatcaa acttttaaga acaattttta
    16201 ttagttggca agtagttttg gatagagaaa aaggaggaag ttccagtcta ttgactagga
    16261 gcaaacaaaa tgaaaataca cacacctatc ggctttttat ggttagatgc agatgaatcc
    16321 ggattgactg ctgtatcttt tcagccaatt tcagaaagcg aatcagcgaa tcagcgaatt
    16381 ttaacgcgca cacaagaaca gtttgaagaa tattttgcgg gaaaaagaaa agaatttgat
    16441 gttcctttat ctataaagag aggtacgcct tttcagcaaa atgtctggaa agctttgtcg
    16501 accattcctt atggagaaat ccgaacgtat aaagaagtag ccgttgctgt tgatagtccg
    16561 aaagctgtgc gagcgattgg gcaggcaaat cgaaccaatc cattgccaat cgtttttcct
    16621 tgtcatcgag tgatcgggca aaatggaaaa ctgacaggat atatgggaaa cgcaatagat
    16681 ggggttgaaa tcaaacgcca attgttgttg ttagaaggat atctgcaaaa ttaaaaacag
    16741 ttgtgattcg gacattgttc catgcgagaa tcacaactgt tttttcatgt tcattctaaa
    16801 ctttgatcag ccaaataaat agtaaagcca tcactgtttg gagaagcccg acagctaatc
    16861 cataagataa gaagcgtgta ccttctttca tgaattttgc aaagtctaaa cgcagaccaa
    16921 ttgccgctaa agcagtgatc tcaaaccagc tgctgataaa atgtgccgat tggtcaataa
    16981 ctacaggtaa agaaaacaag ctgttgacca aacaaaagat cacaaaccct aatacatacc
    17041 aaggaagcgg taattttcgt tttttctgcg tatcagttgg cgtttcaaac agagctggtt
    17101 gtttcttgtg catgaatttt tcaaacagat agacaacaac aacgagcatc ataatacgta
    17161 gaatcttaaa tagcatggcg aattcgacaa cagaattgtc aagtaagctt gccccagcga
    17221 ctacttgacc aacagattgg agtgtaccac ccaaaagtgc acttttttca atgagatttc
    17281 caccaaacaa ctgtaatcca agaaaaggga gagtcaacat catcacagta cccagtagat
    17341 tgactagtgt gatgatctgt cctttttctt cctctttcgc atggatggaa ggggcgattg
    17401 aagcaatagc agaagaacca caaacagcgt ttcctccagc catcatcaaa gaagaagtat
    17461 cgctgaattg cagtttttta ccaatcaagt aagcaccgat gatagtgact gacatttgca
    17521 gtaacacaaa aacaattcct tttacaccaa tttgagcaat cgtttgagct gtgacagttg
    17581 cacctagtaa aaaaacagag aattctaata gtttgctttc agcaaatttt gtgccgattg
    17641 ctagaacagg ctgtttaaaa aaagtatttc ctaataaaat acccaataaa atagcaatag
    17701 tcgctccacc taactgtggc agataaatag caagaagcct gctgaaggca gcgatggcaa
    17761 aagaaagcag taaaccgggt aagatttcgc ggaccgctga atcctttaca gattttattg
    17821 gatgtgacaa tgatttcatt tgatcggttc ctttcgtcaa taaatactaa aagcatactc
    17881 gaacatagta gcactaaacc agtattttga caaaagaaca agaggtcgtg acaaaagtct
    17941 tgtacgacct cttgtttcga ata
//
LOCUS       DAZKSU010000054        17228 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000054 DAZKSU010000000
VERSION     DAZKSU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.054"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..372
                     /locus_tag="ACG8Z3_001899"
     CDS             4..372
                     /locus_tag="ACG8Z3_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0824206.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            552..1493
                     /locus_tag="ACG8Z3_001900"
     CDS             552..1493
                     /locus_tag="ACG8Z3_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0824207.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1562..2293
                     /locus_tag="ACG8Z3_001901"
     CDS             1562..2293
                     /locus_tag="ACG8Z3_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0824208.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2437..4617
                     /locus_tag="ACG8Z3_001902"
     CDS             2437..4617
                     /locus_tag="ACG8Z3_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0824209.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4637..5119
                     /locus_tag="ACG8Z3_001903"
     CDS             4637..5119
                     /locus_tag="ACG8Z3_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0824210.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5135..5896
                     /locus_tag="ACG8Z3_001904"
     CDS             5135..5896
                     /locus_tag="ACG8Z3_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0824211.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6027..7361
                     /gene="murC"
                     /locus_tag="ACG8Z3_001905"
     CDS             6027..7361
                     /gene="murC"
                     /locus_tag="ACG8Z3_001905"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0824212.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7668..8153
                     /locus_tag="ACG8Z3_001906"
     CDS             7668..8153
                     /locus_tag="ACG8Z3_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0824213.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8146..8829
                     /locus_tag="ACG8Z3_001907"
     CDS             8146..8829
                     /locus_tag="ACG8Z3_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0824214.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8955..11600
                     /gene="polA"
                     /locus_tag="ACG8Z3_001908"
     CDS             8955..11600
                     /gene="polA"
                     /locus_tag="ACG8Z3_001908"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0824215.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11645..12481
                     /gene="mutM"
                     /locus_tag="ACG8Z3_001909"
     CDS             11645..12481
                     /gene="mutM"
                     /locus_tag="ACG8Z3_001909"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0824216.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12445..13074
                     /gene="coaE"
                     /locus_tag="ACG8Z3_001910"
     CDS             12445..13074
                     /gene="coaE"
                     /locus_tag="ACG8Z3_001910"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0824217.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13126..13458
                     /locus_tag="ACG8Z3_001911"
     CDS             13126..13458
                     /locus_tag="ACG8Z3_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0824218.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13770..14261
                     /gene="nrdR"
                     /locus_tag="ACG8Z3_001912"
     CDS             13770..14261
                     /gene="nrdR"
                     /locus_tag="ACG8Z3_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0824219.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14284..15657
                     /locus_tag="ACG8Z3_001913"
     CDS             14284..15657
                     /locus_tag="ACG8Z3_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0824220.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15657..16583
                     /gene="dnaI"
                     /locus_tag="ACG8Z3_001914"
     CDS             15657..16583
                     /gene="dnaI"
                     /locus_tag="ACG8Z3_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0824221.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16712..17152
                     /locus_tag="ACG8Z3_001915"
     CDS             16712..17152
                     /locus_tag="ACG8Z3_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0824222.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5876 a         3010 c         3796 g         4546 t
ORIGIN      
        1 cagatgttta cgaatgaaat aaaaatcatg ctttatgtgg acgatgtaga aaaaaatgca
       61 acgttttgga gagcagttgg ctttgcggaa aaagatcgtc aagaaatgga tgggacgctt
      121 ataattgaag taggagttga ggacagtcaa acctcatttc tgttatatga tcgagcattt
      181 gttgagtccc attcacctgg cacagctact agtgccccat ctttgatgtt ttacagtaaa
      241 gatatctttg gactatataa aaaaatgcag acgcaatcag tcacattagg tgatatggta
      301 cagcttggag aagaatatgt ctttaacttt gctgatcccg atggcaacta ctttgcggtt
      361 acaggtaaat aatagaaata aagactttgt ttttctgtgc gaaacaggaa agcgaagtct
      421 ttttaattca aataataata ttatgtaaac aaaaaacttt ccatgcgtaa ttatacacaa
      481 gcaaataaat cgtcaaatgc gaacgccttc tttatactgt ttaggaacga aaagaaggaa
      541 ggaagaaata catggattat caagtactgc tttattacaa ttatacaaaa atcgaagaac
      601 cagaggaatt tgccaaaaat catttagcct tttgcaaatc tttggatctt aaaggtcgta
      661 ttctagtagc tgaagaagga ataaatggta ctgtgtctgg aaccgttgaa aacacgactg
      721 cctatatgaa agcaatgaaa gctgatcctc gatttgctga tacgatgttc aagatcgatc
      781 caagcgaagg acatgccttt aagaagatgt ttgtaagata ccgtccagag cttgtaagtt
      841 taaagctaga agaggacatt gatccaaacc aagtgacagg tgcctatctt tcaccgcaag
      901 aatttaaaga agctatttta gatgaacaga cagtggtgat tgatgcacgg aatgattatg
      961 aatatgactt gggacatttt cgaggggctg tacgcccaga tatacgcagt tttcgagagc
     1021 ttccagactg gatccgcgag cacaaagaag aatttatgga aaaacgggta gtgacctact
     1081 gtactggtgg cattcgttgc gagaagtttt ctggttggct tgtccgtgaa ggattcaaag
     1141 atgtcggaca gctgcatggc ggtattgcta cttatggtaa ggatcctgaa gtccaaggag
     1201 aattatggga cggcaagatg tatgtgtttg atgaacggat cgctgtcgat atcaaccaag
     1261 tagataaaca aatcgtcggt cgcgactggt ttgacggaac accatgtgaa cgatatatca
     1321 attgtagcaa tcctgagtgc aatcgtcaga ttcttgcttc cattgaaaat gaagaaaaac
     1381 atttaggagc atgtagcata gagtgtgcga aacatccgcg caatcgctat gttcaagacc
     1441 atggaatctc tccagaagaa gcagaactgc ggtttacagc tttaattaaa tagaaaacaa
     1501 tagttttgat tgtcgaccat ttccaatatg cgctatacta gcgttgagga tggtgatcca
     1561 catgaaatca ttgatttatg ctattgggga tgtccatggc aaacatgagc tttttgaaaa
     1621 acttattcac tattatgatc caaagatcca tcagcttgtt ttattagggg atctcaatga
     1681 tcgaggtcct cgtacaaaag aatgtttttt attaggaaag caattagtcg aagaaacagg
     1741 agccatttac ctccggggaa accatgaaga atattttttg caatttctgc aatcaccgga
     1801 ggactggtat gcgccttata tgagaaatgg tggaaaagaa acgtttgaaa gcctgattca
     1861 cccaggggca tcggaggaat actccccaac tgaaatctcg atgatgatcc aatcgagata
     1921 ttcagaactg attaaatttt tagtccagcg ccccttgtat tatgaatggg aaaattatct
     1981 ttttgtccat gccggtgtgg atttgacaaa gaaagactgg cgtaagacaa gtctaagaga
     2041 ctttatttgg ataagggaac catttcatga agggaaaaac aatacaggta agacaattgt
     2101 atttggacat acgattacac caatgctcca tggagatatg cagacaacag acctctggat
     2161 tcaggatcat aaaatcggaa tggacggtgg ggctgtattc ggagggtcat tgcatggggt
     2221 tgtttttgac aaggaaggta ttcttcaaga tattgaatat cagaacaacg aaggaccttg
     2281 gcatggaatc tgactaatgg aaagaatgaa ggagctgtga ctttgttgcg gctcttttta
     2341 tttttataga gaatattgtt tctttcagta gtttaaccca ggataaaaag tatgatagaa
     2401 taaagaagca atttttagca gaaaagaggc attttaatga cggaaaattt gaaccagacg
     2461 atcattcagc ttgtacaaaa agaactttca gactatcgtc ctaagcagtt gactactgta
     2521 cttaatttgt taaatgaagg aaacactgtt ccttttatcg cgcgttaccg aaaagaaatg
     2581 actggaagtc ttgatgaagt ccagattcgt gaaatcgaag agcggtatgc ctatcttgaa
     2641 aacttagaaa aacgtaaaac tgaagtgatt cggctaatcg atgaacaagg gaaactgact
     2701 cccgagttag aagcagaaat cacccaagcc gtgaaaatgc aacaagtgga agatttgtat
     2761 cgtccataca aacaaaaacg acggacaaaa gcaacgattg ctaaagaaaa aggacttgaa
     2821 ccactcgcga agtggctgat gcagcttaca gatggagagg tgcaatctga agcagaaaaa
     2881 tatattgaca aagaaaaaga agtctcttct gccgaagaag cgttgcacgg tgctcatgag
     2941 attattgctg aacaagtcag cgataatgca aaatttcgga cttggattcg gtcttatacg
     3001 tataacaaag gtatgtatgt cagcaatgtc aaggatgagc aagcagatga aaaaggcgta
     3061 tatgaaatgt attatgactt tgcagaaccc gtccataaaa tggtttctca tcgaatatta
     3121 gctactaatc gtggagaaaa agaagatatt ctgaaagtat tctttcaagt agatgaagca
     3181 gcaattttag cttacttgga tcgccaaata gtcaaaaatc ctgcctctcc ttcttcttca
     3241 ttcgtcagag aagcctatca ggatagttat aaacgcttta ttcagccagc aatcgagcga
     3301 gaactgcgca atgaattgac tgaaaaggca gatgagcaag caatcgctat tttcggcgaa
     3361 aacctccgta atcttttgtt gcaaccgcca ctaaaaggta aagttgtcct gggatttgat
     3421 ccggcttatc gtactggatg taaactagca gtcgtagatg cgacagggaa agttttagca
     3481 atcgaagtta tttatcctca taaacctgca gcgcaagcaa aaagagaagc tgctggacca
     3541 gcttttatcc aattaattaa taaatatcaa gtagatatgg tagctatcgg aaatggtaca
     3601 gccagtcggg aatctgaact ttttgtggct gagcagttga aatccgccga ccataaaacc
     3661 tattacgcta tcgtcaacga agcaggcgct tctgtttact ccgctagcga aattgcacga
     3721 aaagaatttc cgcatctaca agttgaagaa agaagtgctg tttctattgc ccgtcgcctg
     3781 caagatcctt tagctgaatt agtcaaaatc gatcctaaag ctgtcggagt aggccagtac
     3841 caacatgacg tttctcaaaa acgtttggct gaacaattgg attttgttgt ggaaacagcg
     3901 gttaaccaag taggtgtgga tgtgaataca gcaagcccgc agctcttgca gcatatatca
     3961 ggattgaata aaacaactgc ccaaaacatt gtcatttatc gtgaagaaaa cggagaattc
     4021 accgcacgaa ctcaattgaa aaaagtgcca cgcttaggac caaaagcata tgaacaagca
     4081 atcggattct tgcgagtccc tggtgggaaa aacattttgg acaacacagg tatccatccc
     4141 gaaagttatt ctattgcaaa agaaatatta acttcagtcc aactttcaga aaaagagctt
     4201 ggtacagaag aagcagtaga aaaattgaca cgattatccg tagagaaact ggctgaaagc
     4261 ctatcagtcg gagaagaaac gttagccgat attttagctg ggctgacaca acctggacga
     4321 gatatgcgtg atgagatgcc agcgccctta ttacgtacag atgtattgag tatggaagac
     4381 ttgaaaccag ggatggaatt gactggaacg gtccgtaatg tcattgactt cggcgcattt
     4441 gttgatatcg gtgtgaagca agacggactt gtccatatat cgaaactcag caaaaaattc
     4501 gtcaagcatc ctacagatgt cgtgtcagta ggagatatcg taacagtttg gatagaacaa
     4561 gtcgatacga aaaaaggcag aatcagtttg acgatgcttt ccccttatga agagtaacaa
     4621 tgaggagtaa caaaaattga cacaagaaga actgcagcag ttagtagaag agatatcatt
     4681 aaaaagcttc cagcttcctt tcaagcacca agcaatgttc aatccacgct tacgtacaac
     4741 tggcgggcgc tatcatttaa acgatcatca tttagatttt aatgcgaagc tatttgaaga
     4801 gtcagacgaa gagacgattg caggaatcat caaacatgaa ctttgccatt atcatcttca
     4861 tctagctgga agagggtatc agcataaaga tatggatttc aagtatcttt tgaaaaaaac
     4921 aggagggttg cgctatgcac cagccttgac gaagaaacag ataaagatgg agtattatcg
     4981 atgccaagat tgttctgcca cgatctgccg caagagaaga atcgatgtac gcaaatatcg
     5041 ttgtggaaga tgtcatggag aaatcaaatg gattgagaca aaaagagaag actttaatga
     5101 agcacaaaaa atcagatgaa aggatagaag gcctttggaa aaaagtgagc agaggaatct
     5161 aaaagactgg aatcagctat taaatttttt agccgcacct gaagagggaa attttgagga
     5221 actgacggca cctacactta ttttagctgg aaactgtctt cctatcttag ctgataaagc
     5281 agcacaaatg tatttaaatg gccaagtaga tcaattgttt ttggtcggag gggtggggca
     5341 tgcgactaga atcctttatg agaattttga aaagcaagga tttcattttg aagatggcat
     5401 gagtgaaagt gaaatctgtc gtcagtatct aaaagaagtc tatgatttgc ccgataaggc
     5461 ttttctgata gaatcgaagt ctacaaattc tggagagaac gccacttttt cactggagat
     5521 tcttcattcc ttaggagctg taccagaaaa aatcttattg atgaatgatc cgacactgca
     5581 acgcaggact cgagcaactt ttgagaaagt atggcaaaat gaacaaacta ttttcgcgaa
     5641 tgctgtgcca tttgtgccgg aaatccttca cttttctgaa gagatcatct ttacagcaaa
     5701 agaactgaat catcaatggc ctaaggaata tttccatgcg ctagtattag gagaaatgga
     5761 acgtctccat gacgatgaga acggctatgg tccgaaagga aaagatttta ttcctcacat
     5821 tgatatatca gaggaagttt ggtcttctta caccagaatt aaagagtcaa taaaaacgga
     5881 tttttctcgt acttgaccat tttagcaagc gaatcctcct ttccaaataa atttggttga
     5941 aattttcaat cgtttataag ataatgagga gcgtgttgaa tgtaatagct aaaagaatgg
     6001 acgctagata agagaagagg attcaaatgg aaaatcaaaa taaattgtat cattttgttg
     6061 gtatcaaagg ttcaggaatg agctccttgg ctcttgtttt acatgaacaa ggtctaaatg
     6121 tccaaggatc agacattgaa aaatatttct ttacacaaag agatttagaa aaagcaaata
     6181 tcacgattct gccatttaac gcagataatg taaaaccagg gatgacgatc attgcaggaa
     6241 atgcatttcc tgattcacat gaagaaatcc agcgagcaaa agaattaggt ttggaagtga
     6301 ttcgctatca cgactttatt ggtcatttta tccaaaatta tacgagtatc gctgtaacag
     6361 gctctcacgg aaaaacaagt acgactggac tgctctctca tgtattgagt ggtgtccgtc
     6421 caacaagtta tctaattgga gatggaacag gacatggtga tccgcaagcg gaattctttt
     6481 catttgaggc ctgtgaatat cgccgtcatt tccttgccta ttcaccagac tatgcaatca
     6541 tgaccaatat cgattttgat catccggatt attatacaag tatcgacgat gtatttacag
     6601 ctttccaaac aatggctgga caagtgaaaa aagctatttt tgcgtatggt gacgatgctt
     6661 atttacgcaa attaaaagca aatgtgccga tttattatta tggtgtcaca gaaaacgatg
     6721 atatccaagc acgaaacatc gaacgcacaa cttctggttc tgcttttgat gtctatcatg
     6781 gggatgaatt tgttgggcac ttcactgtac cagcattcgg taaacacaat atcttaaatg
     6841 ctttaggtgt tattgcagta gcttatttcg aaaaactgga tctgaaagaa gttgcagaag
     6901 aaatgctgac tttcccagga gtaaaacgtc gattcagtga aaagatcgta gcagacatga
     6961 ccgtagtcga tgattatgcg catcaccctg cagagatcaa agcaacgatt gatggcgcac
     7021 gccaaaaata tccagataaa gaaataattg cagttttcca accccatacg tttacgcgta
     7081 cgattgcatt gatggatgaa tttgcagaag cacttgattt agctgataaa gtttacttat
     7141 gcgatatttt tggttcggct cgggaagaac aaggaaacgt taagattgaa gaccttggag
     7201 cgaaaatcaa aaaaggtgga gaagtcatca aagaaaataa tgtatctcca ttgttagatt
     7261 atcatgatgc agtagtaatc ttcatgggtg ctggagatgt tcaaaaattt gaacaagcgt
     7321 atgaaaaact tttaagcagc acgacgaaaa atgtactgta atacaattac tgcagtgtcc
     7381 ttataaaaat agaaagaggg ggagtggcaa aaatcttgcc aagccccctt ttttgcaact
     7441 tatgataaac ggtgttcaaa agcttgcatc tgcggtaata agcccaatca aatcaaaaac
     7501 atgagaagct gttttttctt ggctgttctt attatttgat gcaggacagc tttttcacga
     7561 cctctgtaaa ctataggaat ctgttaaatc aagggagcag aacttgcctt ttacgctact
     7621 tttggtacaa taatgtatga ttaaaaataa actggagggt ttctatcttg aacataggta
     7681 agccgagaaa actaggaaaa acaataaatg aaatcgagga aggcgactcg ttatccttaa
     7741 cagaatctat tgaagataac cagcttttgc tgtatttggg attgacgaat gatgcaaatc
     7801 ctttgtatat ccaacatgac tttgcacaaa aaacagaata tcaacagcca atcgttccat
     7861 cagtgatgct gatggggatc atcacaagtg caatttcaaa gcatcttcct ggaccaggat
     7921 cacatgttgt caatttttct gtcaatttta ttgaaccggt tttccattat gaaacactga
     7981 cttttcagtt tgaggtcata aaagtagata agatgaagga tgtggtgacg atttctgtag
     8041 aagcggagaa tacgaacgaa gatcgtgtgt tggatgctgt cgttatggtc caaccgccac
     8101 aagttcctga attggaaata gcaatcgaaa gtgaaggtga attgaatgaa taatccaagt
     8161 acatcaatgt caggtatcaa tcgtttttat gcaaaagttt atggattttt aggtatggga
     8221 cttgccctta gtgcagtcac ttcttacttg gctctttggg tatttccttt gcaattagca
     8281 acttttatca ataattttcc actaggcttt gtgggtctgt ggatcgttga actgatttta
     8341 gtaatggttc taggcgtaaa agcacaaaag aatccgagct tagcaatcgg tggatttatt
     8401 gcctactctc ttttgaatgg cataatatta gcggttactc ttgcatacta cgatattgga
     8461 acagtaacgc aagctttctt gacagctact ttgatgttca tggggatgtc tgttgtcgga
     8521 gtaacaacaa aaagagatct tagcggggtt ggaagagcag gacaggcagc tttaatcgga
     8581 gtgatcattg cactagtctt gaacatcttc attttgagaa gtagcgtagt tgagctgttc
     8641 atctccctac tgttagtcgt catctttgca gggattacag cttatgataa ccaaagcatc
     8701 cgtaatatct atgtccagac aaacggaaca gcacatagtg gaattgctgt attcatggca
     8761 ttgaagctgt atttggactt tatcaacttg ttcttggcgt ttctgagaat ctttggacgg
     8821 aacaactaaa aaagaaaaaa attcatcagt tggggctata ccgaattatt tggtatggtc
     8881 ccgctttttc ttttcgcatt ccgcaggatt ctttgttaga atgaataaga actttacagt
     8941 aaggagttaa gaagatgaca aaaaataaat tattactcgt tgatgggaac agtgtggctt
     9001 ttcgcgcatt ctttgcgctt cacaattctc ttgaacgttt caaaaataga aacggtcttc
     9061 atacaaatgc aatctatgca ttcaatacga tgtttgaaaa tgttatgcaa aaagagcaac
     9121 cgactcatgt tctggttgcg ttcgatgcag gaaaaacgac ttttcggaca gaaatgtatg
     9181 cggagtacaa aggtggaaga tccaaaacac ctggcgaatt caaagaacaa atgccatata
     9241 tacgtgattt gttgacagga ctaggggtac agtattacga attgcccaat tatgaagcag
     9301 atgatattat cggtactttg gcagaaaaag tagacaaaga tcaatttgat gtcgtcgtgc
     9361 tttcaggtga tcgggatttg actcaattag ctagtaaaga agtaaaagtg gatatcactg
     9421 taaaaggtgt cagcgatatc gaaagctata caccagaaca tgtagcggaa aaatatgatg
     9481 ggttgacgcc aaaacagatt attgacatga agggactagc cggagatgcc tctgacaata
     9541 tccccggtgt gactaaaatc ggtgaaaaaa cagctatcaa gctattgaaa caatatggct
     9601 cagtcgaagg aatttatgaa catatcgatg aaatgaagca aagcaaaatg aaagaaaatc
     9661 tgataaatga caaagagcag gctttcttat cgaaaaaatt ggcgaccatc aatacttcag
     9721 cgcctgtgga tgtctctatc gactcattga agtatgaagg gaaaaatctc gataaactcg
     9781 ttccgtttta taaagaaatg gatttcaatc agttcctttc taaattgaat atcgtagaag
     9841 aagaagtaaa aatggatgat attttgtttg aagtcgttca tgaattcaaa gaggaaatgt
     9901 ttactacgga tatggcgttg tacgtggaaa tgatgggcga caattatcat acagaggaaa
     9961 tcgtcggagt agcgtggggg acagaaaaga aaatttatgt cacgaacgat ctatctgtat
    10021 tcgaaaaccg agcgttccat tcttggatca ctgatccgac tcggctgaaa aaagtatatg
    10081 atgcgaaacg aacttatgtt gctttaaacc gctatactgg aaaaacgaaa ggaatcgatt
    10141 ttgatgtctt gctaggtgca taccttctcg atacgaatga aaaaagcaca gatatcgaag
    10201 gaatagctgc tcattacgga tacaacgata tccaatcaga tgaagctgta tacggcaaag
    10261 gggcgaaaaa aggtttacca gaagaagagg aactcttttt tgctcattta gcgcgaaaag
    10321 tagctgcaat caatgcgttg acaccaaaat tgagccaaga actggcagaa aaaaaccaag
    10381 aagatctttt ccggaaaatg gaactgccat tgtctatcat tttggcagag atggaaataa
    10441 gcgggatcaa agtagatgcg actcgcttac aagaaatgaa gggcgagttc tctgctcgct
    10501 taagagagat cgaacaaaaa atctacgaag aagccggcga agaattcaat ttgaattcac
    10561 caaagcaatt aggagtgatc ttatttgaaa aaatgggatt acccgttatc aaaaaaacaa
    10621 aaactggata ttccacagcc gtagatgtac tggaacagct tcgtgaacaa gcaccaatcg
    10681 tagaggatat cctcacctat cgtcaaattg caaaaattca atctacctat gtcgagggac
    10741 tgctgaaagt gatccaatca gatggaaaag tgcatacccg ttatgtccag acattgaccc
    10801 aaacaggacg attaagctct gttgatccta atttgcagaa catcccgatt cgattagatg
    10861 aagggagaaa gatacgtcag gcatttgttc ctagagaaaa agactggctg atttattctt
    10921 ctgactattc acaaatcgag ttacgcgtac ttgcgcatat ctcaaatgat gaacatttga
    10981 aagctgcctt cctagaaggt caagatatcc atgccagcac agcgatgcgt gtatttggaa
    11041 tcgaaaaggc agaggatgtc acaccaaata tgcgtaggca ggcaaaagca gtgaattttg
    11101 gtatcgtgta tggcatctct gattacggac tttcacaaaa tctaggaatc agccgaaaag
    11161 cagcacagca atacatcgat acgtactttg aaaaataccc aggtgtcaaa gaatatatgg
    11221 aaagaatcgt acgcgaagca aaagaccaag gctacgtcga aacgctgtat caccgccgtc
    11281 gatatctgcc agacatcaat tcaagaaatt acaatatccg ttcttttgcg gaacgaacag
    11341 ctatcaatac accaatccaa ggaagtgctg ctgatatttt gaaaatcgcg atgatcgaat
    11401 tggataaacg attaaaagaa acaggtcttc aagctacgat gctgttacaa gttcatgatg
    11461 aacttgtctt cgaggtaccg gaaaaagaac ttgaatcatt agataaattg gtgaaggaag
    11521 ttatggaaca agctgtttct cttcatgtac cactgatcac tgacagcagt tggggaaaaa
    11581 cgtggtacga agcaaaataa acattgttta agacagagca atataatgaa ggtgaaaaga
    11641 aaaaatgcca gaattaccag aagtagaaac cgttcgtaaa ggattggaac ggttagtagt
    11701 aggaaaaacg attcaaaaag ttcaggtctt gtggcccaag atcatcgagc aaccagagac
    11761 acccatcttt gaggcttctc ttgttggcga aaccatccaa tccattggac gtagagggaa
    11821 gtttttaatt tttcatctcg atcattgtga actgatctca cacttgcgaa tggaaggaaa
    11881 atatcaattt acgaaagaaa atacaccaat cgataaacat acgcatgtac ttttcttttt
    11941 tgaggatgga agccaacttc gctacaacga tgtacgaaaa tttggtcgta tgacaatcgt
    12001 cgaaaaagga gcaagtgcaa cttaccgagg aatcatgaaa ttgggacctg agcctttgcc
    12061 agatagcttc ttattagcag atttcgcaaa tggactgaaa aagtcccaca aagcaatcaa
    12121 gccattgctg ctagaccaac gtctagtgac tggtctaggt aatatttatg tagatgaagc
    12181 attatgggaa gcgaagatcc atccagaaca accggcgaac acactgcgcc ccaaagaggt
    12241 agaacaatta cgtctatcga tcattgatgt tttggatcgt gcaatagaag ccggaggtac
    12301 cacgattcgc agttatttga atgcattagg agaatcaggc ggatttcaag tagcactgca
    12361 tgtctatcag caaactggca aaccatgtat acgttgtgga acgccgatcg tgaaaacaaa
    12421 agtggcacaa agggggacgc actattgtcc gaattgtcaa aggctaaaaa tcgcaaagta
    12481 ggatttgttt taggtttgac gggtggaata gctaccggta aaagtacggc agccaaagtc
    12541 tttcagtctc atggatttcc tttgatcgat ggcgatgtga ttgccagaga aactgttgag
    12601 cctggaacgc ttgctctgag gaaaattgta tcagtgtttg gccaagaggt attgcagcct
    12661 gatgggcaac tagatcgagg gaaacttggg atgattgttt ttccatcaaa ggaaaaacgg
    12721 caaaaacttg atcagctatt agatccgttt atccgaaaag cgataaaaga tcaaatcgcc
    12781 tctctaagtt ccaatcatcc tttagtaatt gtagatatcc cattgctcta cgaaggtcat
    12841 tatgaccact atatggatgc agtagctgtc gtttatacta cgccagaaac tcagcttcag
    12901 cgtttgatga aacgtaatca actgacgcta gaacaagcgc agcagagaat tagtagtcaa
    12961 cttccaatcg aagaaaaaaa gcagcgtgcg gatattttgt tcgacaacaa tggaacaaaa
    13021 gaaatacttg tgggacaaat cgaaaattgg ctttttgcta atcgctttct ttgagtatct
    13081 gtgtcaattt tgctatcctg agtatataga aagtgaggga tagcgatggg atcattaaaa
    13141 aatcaaaagg ataaagtcgt aggtaacgca aaagaaaaaa tcggcgagat gctcaataaa
    13201 gaaaatctaa tcgatgaagg tcgtacgcaa tcagaaacag cagaagaaaa agaaaagcag
    13261 taccaagcaa gaatcgatga aatagaagac cagcaggcag aagaagaagc gaagcggcaa
    13321 gatttgactc aagaacgaaa aaatccgaca cctgatcgta gtaaaacacc gctcccagaa
    13381 agtgaccaca ttcccgatga gcaaaaaatc gaggaacagc gaatgaaaca ctatacagga
    13441 atcgaagaga agttataaaa tagtaaaaaa tcgagatgtg acttgaagca gatgagaagg
    13501 tgttcatctg cttcaagttt tttgtgctag atgactaaat cagcttttgt gtaagttatt
    13561 tctcagtaat caaggtgatc agtattggaa aattgaaatt tagtaaggac aggggacaca
    13621 attacgcatg aaagtagaaa aaattgttct tatatccgtg ttttttgggt aggacagtcg
    13681 cgtttgtgat ttgtttcgtg ttataataca actagttgta aagaaagaca catcctttat
    13741 ataaaatgcg atgcaagagg tgagacaaaa tgagatgtcc cagatgtcaa cataataatt
    13801 cacgagtaat cgacagccgt caagcagatg atggacgtgc gatccgtcgc cgtcgcgaat
    13861 gtgagaattg ttcgttccga ttcactacat ttgaacgaat tgaagctgct ccgttattag
    13921 taatcaagaa aaacggggaa cgcgaagagt tcaatcgcga taaaatttta agaggactca
    13981 tccgttcagc agaaaaaaga cctgtagcta tggaacaaat ggaacaaatc gtcaaccatg
    14041 tcgaaaaccg cgtacgcagt ctcggagaaa acgaagtatc gacgaatcta attggtgaat
    14101 atgtgatgga agatttagtt gatttagacg aaatcgctta tattcgtttt gcaagtgttt
    14161 atcgccagtt caaagatatg tctgttttct taaaagagtt gcaagatatc gtggataaag
    14221 caaaagaaaa taataaagag aataataaag aaaacaatta agaaacaaaa aggaggcatt
    14281 tccttggaaa atgcttggaa agagcttcaa ccaaaaaata tttaccaagg aagaaaagcg
    14341 gctccgctga gagaagacag caagaacgcc ttgctgtatc tttatcagcc actaatcggc
    14401 ggagaagcct tgagtatcta tttcaccctg ttatcagaaa tctctcaaga atctggcaaa
    14461 ggtcctgaag ggatgcatgc agatctgctt agtagtctag gctgcggtat cccgcaattt
    14521 tatgatgcaa gaaaaaagct agaaggaatc ggcttactgg atgtttattt taaagaagat
    14581 agtgatctgg gacctcgttt tatttatgag ctgttagaac cactcattcc taaagcattt
    14641 ttcaaagact ctgttctggc ttttcttctt ctggaaaaag ttggtgaacg acgattccag
    14701 aagcttaccc aattctttga acctaaaagg catacagcag agggatatca aaaaatcaca
    14761 aaaaaatttt tagatgttta tcgcttcaat gaagcagtgt acgctgctaa tcaagaaaac
    14821 atcgaatcgc tgaataaatc ttttgaagaa tctgaatcag tagcattact tgaagcagat
    14881 tcgacattcg attggcagtt tttgaatgat tggctggcaa aaaaacatat tgcaagtctt
    14941 gatgatccaa tcatcaaaca agtcgacatg tatcatcagc tttacggttt cgatgaattg
    15001 acacttggcg aactgatcag tcaatcttat gactttagtg aacaaaaagt atctgaaaaa
    15061 gaactgcaaa aagccatttt ggcaatgcag agacgtccgg tgaagcaaac aaaagaaaca
    15121 gttgcggttt ctgaaaacag taatgaaccc tcgtcaaacg aacatttgac aaacgcagcg
    15181 cttcagctga taaaagaagc acagagcatt ccaccgatga aatatctgga agcaatcaag
    15241 tcagaaaaag gcggatatgt ttctaaacaa gaaaactggt tactgcaaga tcttgtttct
    15301 caatcaggtc tttccagtag cgtgatcaat gttttgctga attatgtact agtgatcaag
    15361 aataatgcta gcttaaatgc cagttatgta aatacagtgg caaatgaatg ggcacaaaag
    15421 aaaatcgcca cagcagaaga agcgatccag cacattcggc aaataagtaa tcaagcaaaa
    15481 caatcaaaac aaaagaagca aacaaattat caaactggca agcgaaatgt tcgacgggaa
    15541 aagcttcctg attgggtcaa ccagccaaaa gatgaaaaac agatcagcag cgagaaaaaa
    15601 gcagagattg accgccgttt taaagaatac ctggcaaaaa aggagggtga tatctagtgg
    15661 aagacgtagg caaagaattg aagaggatca tcagcaaacg caattatcaa gaaaagtatg
    15721 ctgaaatgat cgctgaagtc ttaaatgatg cggatgttca agcttttcta agcgcacacc
    15781 aagaagaatt gacacaagcg gatattgaaa aaagttacgc taaattgtat gaattcgtgc
    15841 aagaaaagcg taaatatcaa aaaaacgatc caacgatgat cgctcccggc tatgagccga
    15901 agctgacgtt gaattttcat tatgtggatg tcacttatgt accaacggaa gcattgttag
    15961 cacggcaaag acaagaagaa atcagaagcc gagtaaaagc aatggatatg ccaaaagata
    16021 ttcaatcggc ttcttttgaa aactttgaac gtacagatgg acgaggatat gcgagcttag
    16081 aagccctaga ttttgtagag aaatatactg ctgatccaaa agggtatcat aaaggactgt
    16141 atctagtagg aagttttggt attggaaaaa catatttatt aggagcaaca gcgagagaat
    16201 tagcgattgc tggattcacc acgacgctag tccattttcc gacatttgcc gttgagatga
    16261 aacaggcaat cggaaaagat caagtggcag aaaagttaga tgcagtgaaa cgttcaccta
    16321 ttttgatgat cgatgacatt ggtgcagatg cgatgagtag ttggatacga gacgaagtat
    16381 tcggtgttat ccttcaatat cggatgcagg aacagttacc aaccttcttc tcatcgaatt
    16441 ttacaatgga tgaattggaa cagcatttgt ccgtcactca acgtggagat gaagagccgc
    16501 taaaagcgaa acggataatg gaacgcatac gttatttgac aaaagaaatc gaaatgactg
    16561 gtcgcaaccg acgcaatggt tgaacagttg acaagtccaa ctgacatttg acctagtagt
    16621 aaaagcaggt cagcgaatca ttatatggac agaaatgacc ggagacaatc accgcaacaa
    16681 gtaatgcaga aacttaaacg gaggattttt tatgagggct attttttcta gtatatctgt
    16741 agttggtcta ttttttcttt tagcaggctg cggtagcgca gatagcaaca caggttctgt
    16801 tgattcgatt gatgcgggtc aaacagtaaa aacaagcagt acgaaaacgg agagacaaag
    16861 catatttgtc ggaacgattg aaagtatcac tcaagtagaa gcagaagaag aaacgcttca
    16921 gttgaaactc gtcgatgtca tggccaaaga agatccagcg aatatgggaa cgtcattcag
    16981 caatgacgga gtgatcttaa atgctactgc tgaaaaaata agcgtggaca taaatgaatt
    17041 gaaaatcggt gacaaagtcc aatttacatt agttgagatg ccgatcatga cgatgtctat
    17101 cccaccgcaa gttcctggag attcgattat tgatgtttca ttgaacaaat aaccaataac
    17161 attttttcaa aaataagaaa atgttgtttt atagaacgaa aaggtatatt ttttaaggag
    17221 atatggga
//
LOCUS       DAZKSU010000055        17018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000055 DAZKSU010000000
VERSION     DAZKSU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.055"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2435)
                     /gene="parC"
                     /locus_tag="ACG8Z3_001916"
     CDS             complement(<1..2435)
                     /gene="parC"
                     /locus_tag="ACG8Z3_001916"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0824223.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPE"
     gene            complement(2447..4492)
                     /gene="parE"
                     /locus_tag="ACG8Z3_001917"
     CDS             complement(2447..4492)
                     /gene="parE"
                     /locus_tag="ACG8Z3_001917"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0824224.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(4658..5092)
                     /locus_tag="ACG8Z3_001918"
     CDS             complement(4658..5092)
                     /locus_tag="ACG8Z3_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0824225.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5219..5857
                     /gene="plsY"
                     /locus_tag="ACG8Z3_001919"
     CDS             5219..5857
                     /gene="plsY"
                     /locus_tag="ACG8Z3_001919"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0824226.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(5989..6864)
                     /locus_tag="ACG8Z3_001920"
     CDS             complement(5989..6864)
                     /locus_tag="ACG8Z3_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0824227.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(6946..7737)
                     /gene="codY"
                     /locus_tag="ACG8Z3_001921"
     CDS             complement(6946..7737)
                     /gene="codY"
                     /locus_tag="ACG8Z3_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0824228.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(7755..9155)
                     /gene="hslU"
                     /locus_tag="ACG8Z3_001922"
     CDS             complement(7755..9155)
                     /gene="hslU"
                     /locus_tag="ACG8Z3_001922"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0824229.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(9169..9717)
                     /gene="hslV"
                     /locus_tag="ACG8Z3_001923"
     CDS             complement(9169..9717)
                     /gene="hslV"
                     /locus_tag="ACG8Z3_001923"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0824230.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(9754..10659)
                     /gene="xerC"
                     /locus_tag="ACG8Z3_001924"
     CDS             complement(9754..10659)
                     /gene="xerC"
                     /locus_tag="ACG8Z3_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0824231.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(10857..12233)
                     /gene="trmFO"
                     /locus_tag="ACG8Z3_001925"
     CDS             complement(10857..12233)
                     /gene="trmFO"
                     /locus_tag="ACG8Z3_001925"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0824232.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(12202..14280)
                     /gene="topA"
                     /locus_tag="ACG8Z3_001926"
     CDS             complement(12202..14280)
                     /gene="topA"
                     /locus_tag="ACG8Z3_001926"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0824233.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(14402..15259)
                     /gene="dprA"
                     /locus_tag="ACG8Z3_001927"
     CDS             complement(14402..15259)
                     /gene="dprA"
                     /locus_tag="ACG8Z3_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0824234.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(15315..16082)
                     /locus_tag="ACG8Z3_001928"
     CDS             complement(15315..16082)
                     /locus_tag="ACG8Z3_001928"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0824235.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(16075..16935)
                     /gene="ylqF"
                     /locus_tag="ACG8Z3_001929"
     CDS             complement(16075..16935)
                     /gene="ylqF"
                     /locus_tag="ACG8Z3_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0824236.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         4330 a         3801 c         2959 g         5928 t
ORIGIN      
        1 atctcaggag catcgataac ttcgatgact tctccacctt ttttctcatc catcgcaaat
       61 gatccattcg atgttcgatc acctataggg aaatcagttg ggtcaatcgt taactgctgc
      121 cctttttgat tcatcaaggt tatttctttt gatcttcctt ccgacatata gataatacgg
      181 tgcggatttt ttttcagctc acgcatgacc gtcactcccc gcttcgcacg tgaagtttgg
      241 ctgatttctt gagcaagcat gcgtttaaca gctcctcgct gagtaacgat tacgatcggc
      301 gtatcacctt ctgctaacac aagcagcccg ttgataatcc tatctccatc tttcaaattc
      361 atagacttca ctccagctgc ctttgctcca acaacgggaa catctgttaa aggatagcgc
      421 aaacccattc cttgatgcgt tactaagaaa acatctaagc ctggctgatc ttctttcaga
      481 tacacagcaa tcagttcatc ttcttccgcc tttagcttca tacagctgag cggacggctc
      541 ttataggtgc gccatggttc gaactctgtc attcgtgttt gcttgatcaa tccattcttg
      601 ctgatgaata caaatgtttt ctctgcttga attttttgat atgggaaaac agctaaaatc
      661 gattcgtcta cagacagatt ggagattgtt tgagaaatgt gctcaccagc atctttccat
      721 ttcaaatcgg gtagttcatg gacagggcgg tagatcacat ttgctttatt tgtgatcaga
      781 agaacatggt ccaatgtact caattcacct gagtatagca agtagtcgcc ttctcgcatc
      841 ccgatttctt ctggtttgga agcactataa gaacgaaggc ttgttcgctt cacatagcct
      901 tctcgcgtca cactgacaat tacgtcttct tgtgccacta agacttgcgt atcaattttg
      961 atttcttcga tttcgtcttc gattgtcgtt agacgagcgg aagaataatt ttttttcact
     1021 tctcgcaatt cttttttcat aacagagaaa agctctttgt catttgaaag gattttattc
     1081 aactctgtga tttgcgcaat caagctttcg gattcctttt gaagctctgt gatatccgtg
     1141 ttcgtcaaac ggtataattg caatgtgaca attgcttctg cttgttcttc tgtaaattga
     1201 aagacgtcga ctaaattctt tttggcgtct tttttgtctt tactttcacg aatcgttgcg
     1261 attacttcat ccagaatcga taaagctttc atcaatcctt ctacgatatg ctgtcttttt
     1321 tgagctttgg ctaaatcgaa ttgactacgt tttgttatga cttgctttcg atgctcgata
     1381 taacttctta aaatgtcttt cagtccaact tgatgcggag tcatatggtc gatagcgacc
     1441 atattgaaat tgtaattgat ctgtagctca gtgtttttga acaaataatt caaaattcct
     1501 tgagcattgg cgtctttttt caattcgacg acaatttgca gacctgttct atcactttca
     1561 tctcgtactt cagctattcc atcgattttc ttattcaaac ggatctcatc catttttttc
     1621 accaaggtcg ctttatttac ttcatatggg atttctgtga tcacgatttg ctgtttatta
     1681 cccttcattg cctctatctt tgttttcgaa cgaagaataa cttttcctcg tcctgtttca
     1741 taagcttttt tgatttcatc ttttccttgc aagatccctc ctgttggaaa gtcaggtcca
     1801 ggaatatatt ccatcaattt ttcaaggctc gcttgtggat gatcgatcat atagattgtg
     1861 ccatcgatca cttcagctaa gttatgagtc gggatttctg ttgcatatcc agcagatata
     1921 cctgtcgatc cattgacgag aaggtttggg tatcgagctg gaaggactgt tggttctttt
     1981 tctgtatcat caaaattcca cacgagatcg actgtttctt tctcgatatc tgctagcatc
     2041 tctccactta acttggataa tctggcttca gtataacgca tcgcagcagg tggatcaccg
     2101 tccatactcc cgttattccc gtgcatttcg atcagtactt cccgcaactt ccagtcttgg
     2161 ctcatgcgga ccatcgcttc ataaatactg atgtcaccat gaggatgata attccccatg
     2221 atatttccga cagattttgc tgatttacgg aatcctttgt catacgtatt gccatctttg
     2281 ttcatggcaa ataatatccg gcgttgcact ggcttcaatc catcacggat atcaggcagt
     2341 gcacgttctt ggataatata tttggaataa cgtccgaagc ggtcacccat cacttcttct
     2401 aaagttaatt cttgtacttc ttgtcgattt tccatcaggt gacctcctat tctacttcaa
     2461 ataactgatt gttttcattt ttatctgctc gttcttcatc taataaatca ttagaaactg
     2521 aaggagagat ttcagtatcc tcttttttat ctaagatact gccatcttct tcgagcgtaa
     2581 attgcacgtg attttcgatc cattttcttc gaggttctac tttgtcaccc atcagtgtgg
     2641 ttactcgacg ttcagcttgc gcagcatcat cgatacggac gcggatcaac gtacgggaag
     2701 tcggatccat cgttgtttcc cacaattgtt ccgcattcat ctctccaagt cctttatatc
     2761 gctgcagcat atatcctttg ccgacttttt taatcattgc tgccaattcg tcatctgtcc
     2821 acgcatattc aatgacttgc ttctttcctt gtcctttgga tactttatat aaaggcggta
     2881 acgcgatata tactttccca gcttcaatca gcggtttcat ataacgataa aagaaagtaa
     2941 gcagtaatac ttgaatatgg gcgccgtctg tatccgcatc ggtcatgatg attactttgt
     3001 cgtaattaca gtcttcgata gaaaactcag gaccgactcc tgcaccaatc gtatagatca
     3061 tcgtattgat ttcttcattt ttcaagatat cttgcatttt agctttttcg gtattgatga
     3121 cttttcctcg taaaggtaga attgcttgga actttctgtc tcttccttgt ttagcagatc
     3181 cgccagcaga atccccttcg actaaataca attcattttt cttagggttt cgggattgtg
     3241 cgggtgtcaa ttttcctgaa agaagagatt cgcctttttt acgcttcttc ccatttcggc
     3301 tttcttctct ggctttacgt gctgcttctc tcgcttctct ggctttgatt gctttacgga
     3361 ccaacatctg gctcatttcg ctattttcct gcaaataaaa gcccatttgt tcactgatga
     3421 cgttatctac tacggtacga gcaactggcg taccaagttt ttcttttgtt tgcccttcaa
     3481 attgcagaag attttcaggt acacggatag aaagtacagc agctaaccct tctctgaaat
     3541 cactaccttc cagattcttg tcccgttctt tcagcaaacc aacttttctc gcatattcat
     3601 tataagattt cgtcatcgcc gctttcatac cagcttcatg ggttccccca tcttttgtcc
     3661 gaacgttatt gacaaaagat aaaacatttt ctgaataacc gtcattgtat tgataagcga
     3721 cctctacttc gataccttct ttttctcctg aaaaataaac aactggtgtc aatgtatctt
     3781 tttcttcatt cagataatca acaaattctt tgattccttc ttcgtaatga aaaatttcct
     3841 tgactggttc ttctccacga agatcagaaa gagagatttt aacgcctttt agaagaaagg
     3901 cagattctct taaccgttcg gctaaaattt catatgaaaa tttcgttgta ctgaatattg
     3961 tatcatctgg taaaaaatgg acagtcgtcc cgtttgcttt ttttgttttt cctatctttt
     4021 tcaaagtacc gtcaggttta ccgccgtttt taaatttctg ctgatactct acgccatctc
     4081 taacgatcgt tacaattagc catttcgata aggcatttac cacactggct cctacaccgt
     4141 gcagtccacc agaagttttg taccctcctt gaccaaactt tccgccagca tgcagcacag
     4201 taaaaatcac ttcaactgtt ggtattccgg aagcatgcat tcctacaggc atcccacgtc
     4261 cgctatctgc aacagttata ctattgtctt catggatggt cacatcaatc tcactgccat
     4321 aacctgataa tgcttcgtca actgcattat cgacgatttc atagactaaa tgatgcaagc
     4381 ctcggctgtc agtggatcca atgtacatcc ctggtctttt tctaactgcc tctaatcctt
     4441 ccaatacttg gatggacgag tcattgtatt cgttgtttac tttttttgcc aaggcaaacg
     4501 ctccttcaat aatactttgc ggcaaaaccg caccacgatt atcatactag aaaaggtcgt
     4561 gaaaaaaaag agggtagacg agaaatcaat tttaattatc tcctaaccgc tttttcgaca
     4621 ttcccctcta tcgatcatct cttctttgtg gtcattttta tttttggttt tctttcaact
     4681 taccaagttc aatcttaata caacgatcca ttacgatatc tgtacgtcct gcttttctta
     4741 gcatctcagc tgcttctttg ttttcaatcc ctaattgtgc ccagaacact ttagcatcag
     4801 ttttcaaaaa atcacctgct atttcaggta aaaattcact ccgtctaaat acatctacga
     4861 tatcgattgg atcatcgatt tctgtcaaat tcgcatagac tttctcgcct agaatttctt
     4921 caccagccaa taatggattt actggtatga ttttatatcc attgtcttgc aatacctgtg
     4981 caaccatgta acttgttcgt tctggtttat tgctcaagcc tacgacggca atattttttg
     5041 cttcttccaa atagttgaaa atcgtttctt gtgttggatt ttttacagac atttctgcca
     5101 ccccttgctt ttctttttat tccatctatt atacttgtat ctttcatttt ttcaattgtt
     5161 cctgttacct attcatgcta aaataaagga tagttttcaa cttgaaagag ggtaaaaaat
     5221 gaaattgatc ttattactta ttttagcata tcttttaggt tctattcctt ccggtgtctg
     5281 gattggcaaa ttgtttttca aaaaagatat ccgacaacac gggagcggta atactggaac
     5341 gaccaataca tttcgtgtat taggcaaaac agccgggatt gttgtcctgc ttatggatat
     5401 tttaaaaggt acattggcta cttgtttgcc gatgatcttc cacctttctg ggattaaccc
     5461 tctttggttt ggggtatgcg ccattcttgg ccatacattt ccgatttttg ctgggttcaa
     5521 aggaggaaaa gctgttgcga caagtgcagg aatgctttta ggatttaatc ctgtattttt
     5581 tgtttattct tgtatcatct ttatcgttag tttattttgt acaagtatgg tgagtttgag
     5641 cagtatgatc agtgcagtat tgatcacttt atctacgatc gtccttcctt atgtagctcc
     5701 tgtgattttg gctaagccca attggttact gacgatcatt gcctttttag tttcttcctt
     5761 tatcttttat cggcataaag acaatatcaa gcgtatcaga aaccatactg aaagtcgaat
     5821 cccgtttggt ttgaatagca gcaagaaaaa atcctgatac tttttcattc caaagagata
     5881 aaacgggagt gtgacaaatc gatgtcacac tcccttatta cttacaggat agctttttta
     5941 cacaccttgg taaacggtgt tccctgctaa cttctcggtc acaacctctt acttcactgt
     6001 gatggaatat tttgtcttga agatttcatg ggcacctagt tgattgatcc ctactttttc
     6061 catcaagttt ccactactgt tcaccgtgtc agcgattccg caccacggtt cgatacaaac
     6121 gaaaggcgct tcttttggat aaggagacca gatccctaca taaggcatat ctttatagct
     6181 taaagttacg ctatgggaag atttttcact tcgaatggcg tacgcattta gaccccttgt
     6241 ttcaaaaacc aaggcatctt gctcaaaaag ttcacgattc aaagccagat tcgtatttgt
     6301 ctgacctaac gtcttttgat ccatatcgat gaatgggcct ttcaatggta ggcgtaatct
     6361 tgatttcaat ggagaaaatg ccagatagaa atcttcaaat gacaatcctt cttcaagagg
     6421 tacattgaaa gcaggatgtc caccgataga aaagaacatt tctttttcac cggtatttgc
     6481 cacttgataa tgaaccgtga tgccgtctcc tcctaattca tagctgatca ctaattcaaa
     6541 atcaaatgga tacttttcta atgtctccgg cgtactcctt aagatgaaac tagccatttc
     6601 ttgtccatgt tcaatgactt caaaatccat atcccgagca aatccgtgtt gccccatttg
     6661 atatgttttc ccttgataaa catactgatc atcttttaaa cgtccaacaa aagggaataa
     6721 aaccggtgca tgtctgcccc aatacgctgg atcaccttgc cagatatact ctaaaccatg
     6781 ttcttttgat ttcagactga tcaattccgc accgtgttca gcgatttcgg cgatcagctg
     6841 gtcattttga ataataacag tcattgccat tcctccaact tcgacttact ttttaaaatg
     6901 ggaaggctgg aacaaacatt tctatctgcc ccagtcccct tacttctaat aactgtgttt
     6961 tgctaactcg tctttaaaac gggaattcaa tacttttaaa taagttccct tcatgcctaa
     7021 cgaacgagat tcaatgattc cagctgattc caattttcgt aaagcattga cgatcacaga
     7081 acgagtgatg ccgatactat cggcaatatt agaagcagtc agacgacctt catctccatc
     7141 caatgcttca aagatagctt gtacagcctt caattcgcta taagataatg tgttgattgc
     7201 catttggaca gccgttgcac tccgtacatc tgcttcgatg cttcgagact tctgataaag
     7261 gatctgcata ccgacaaccg tagctccata ttctgctaag actagatcgt catctgcaaa
     7321 cgattgttcc atacgagata agataatcgt acccaaacgt tcgccagctc caaagatcgg
     7381 aacgacagtc gtcagaccaa atggatattt ctctcttgac tctaccggaa atgcagtaat
     7441 atcactagta attgggatat tagcttcagt tttcatcaac tgtgccactg tatcagtata
     7501 agattcagga aattgtttct caacaaacat tgttttgatt cgttcattgt tcacatcgtg
     7561 acgttcatta taacctagta atactccgtc acttcctatg atataggtgt tgctgtcaag
     7621 aataccacct agtgtgacag ccattttatc gtaaggcaaa tctgcttgca tgtcaaaagt
     7681 attcttttgc tgcaaaagtg tgtttatctt gcgggttttc gttagcaagt ctgtcattgt
     7741 cgtcctccga ttttttataa aatatagcga cttaaatctt catttgccgc aattgagcca
     7801 agtttgtcat ccacatatgc ttctgtgatc gttatttctc ccatttgcat atctggtgct
     7861 tcaaataata gatcttctag tagtttttcc aaaatggtat gcagtctgcg tgcgccgata
     7921 ttatctgtgt cacggttgac atcgaaagcg atttgagcga tccgttcaat tgcttctttt
     7981 gtaaacgtaa ctgacacatt ttctgtaccg atcaacgcga cgtattgttt tactaaagca
     8041 ttatttggtt cggtcagaat cttcacaaaa tcttcagctg tcaaatcatc taattctacc
     8101 cgtatcggga agcgtccttg aagttctgga attagatcac ttggtttaga caaatggaaa
     8161 gctcctgaag caatgaatag gacgtgatct gtttggatga tcccgtattt tgtgtttact
     8221 tgtgaacctt cgacaattgg caatatgtct ctttggacgc cttctctgga tacttctcca
     8281 gattgctgtg atttagaagt gatcttatcg atttcatcga taaaaataat tccactttct
     8341 tccgctaatt tgattgcctc actatggata tcagcatcct tgactaattt tgctgattct
     8401 tccttgatca ataactctaa agcttctttg actgttaccg tccgttccac ttttttcttc
     8461 ggtgtcaaag cacctagagt ttcatttagg tcgatcccca tttgctccaa tccattgttc
     8521 atcattggtg caggtttctt ttggtcttca acttcaatgg ttacttcacg actatccagt
     8581 aaaccctttt gtagttgttc caagactgta cggcgattga ttcgaatatc ttctgtcact
     8641 tcttcttgcg gttcagcggt attttgctga ctagcattaa aaagattcat catttgttca
     8701 aattgattag tggattgttt ctgttctttt ttgataccag gtaccaagat ttttaccaaa
     8761 cgtttttctg catttttttt agcacttgtg tatacacggc tgtactgctc tttttctacg
     8821 atttggatcg cattttctac taaatcccga accattgatt ctacatctct tccgacataa
     8881 ccgacttcag taaattttgt tgcttcgact ttgacaaatg gtgcattgac gatttttgcc
     8941 aaacgtcgtg cgatttctgt tttaccgacc cctgtaggtc cgatcatcag catatttttt
     9001 ggtgtaatat cttgttgcat tttttcatct aactgcaaac ggcgatagcg gttacgcaaa
     9061 gcaacggcaa cagatttttt tgctgcgtgc tgtcctacga tatattcatc taattcactt
     9121 acgatttgtc ttggtgtttg ttttaattca tacatatcta ctcacctctt acagttcttc
     9181 tacgataata ttatgattag taaagataca gatatctgcg gcaatattta acgcattttc
     9241 tgcaatttct ttcgcactca tgtcgttttg agcatagttt ttcattgctc gggctgcagc
     9301 caacgcaaaa ttccctcctg atccgatagc taagatacca tcatctggtg tgataacttc
     9361 tcctgtacct gaaacaagaa gcatttcttt atcattcatt acgatcagca tcgcttcaag
     9421 tttttgcata gattgctgtg tccgccattc ttgagctaat tcgacagctg cgcgttgtag
     9481 attcccgtta tattcgttta gtttgccttc aaatttttct tctaaagtaa acgcatctgc
     9541 aacacttccg gcgaaaccta caacaacttc tccattgtag atacgtcgta ctttctttgc
     9601 agtacctttc atgacaacag attcacccat tgttacttgg ccatctccag ccatagcaaa
     9661 tttcccgtct ttttcgacgg cacagatagt ggttgaatga aatggtgatt cgaccatgtt
     9721 tgtttcctcc taaatttgtt cttttcctta tttttacgct cttggatgga aagaacgata
     9781 gtttttttgt aaactttctt ttgttacatg ggcatagatc tgggtagtcg ataaattggc
     9841 atgtcctagc aattcttgga ctgtccgcaa atctgcccca ttatttaata aatgagtagc
     9901 aaatgtatgg cgcagcatgt gaggatggat atcactatcc aaactgcttt ttttgatcat
     9961 ttgattcaag acatattcaa ttccagtagg cgtgattggt tttccatgat gattcacaaa
    10021 aacgatatca tgctcttcgt gatattttgt catcaatacc tggcggcctt ccctgaaata
    10081 ttcttgcaga gcatcttgag caaaagaacc tagaggagca tatctctctt tgttcccttt
    10141 accatggatc aacatgacct ctgaatcaaa atcaatgtct gataccgaca aactgctaca
    10201 ctcacttaaa cggatgcctg acccgtaaag gacttccagt aaagcgcggt tcctcaaatc
    10261 caaaggacta tctcctgcta cgctgtcaaa aagtgcttgc atttcatttt cataaaaaaa
    10321 acgtggcagt ctcaaatttt tctttttgag atgaacgtaa gaaaatggat tttcttttat
    10381 cacttcttct ttgagaagat actgataaaa cgaacgtagg ctggctatct tgcgactgat
    10441 agaatttctg ctatagctag gacgatgatc agctaatgca ctcaaataga cacgaaccgt
    10501 aaaatattcc gccttcagca gatcagcgtc tccagattcc tccaaaaaat gtttgaaatg
    10561 gagaatgtct tcttcatatg cttctttcgt tttttctgaa tatcctcttt cgacaatcaa
    10621 ataacgcaaa aatttttgtt cccacgtggc ttctgtcatt ttctcacctt ccttttgtcc
    10681 cagagaataa tttagcacag cgtttataaa aagacaaata aattgtcaga atttaaccaa
    10741 tatttctaag tttgttcaca attggcgcaa ttcctcacat tttgtttgag aaattcttaa
    10801 gaaaaacgca agaaaaagag cgagactatc cgtctcaccc tcttcatgga gtttccttac
    10861 tcatttggga ttgcagtttt ttctaattct gtttttattg cctcattagc ttttaaagct
    10921 cgttgtgcaa ttgcttcata tcttgctttt ttgtctcgaa tgcgctctgg cagttcagga
    10981 aacagaccat agttggcatt catcggttgg aaatgttttc cttccgcatg agtaatgtag
    11041 taagccatac ttccaatagc tgtttcttgt ggaaactcga ctggttccat ttcttttgct
    11101 aagcgtgcgg cgttgattcc agcaagtagt ccgctcgctg cactttcaac atagccttct
    11161 acaccagtca tctgacctgc aaagaacaga tcttcgcgtt tcgttgattg gtaagtcggt
    11221 ttcagcaatt ctggcgaatt cataaagctg tttcggtgca tcacaccata gcgaacgaat
    11281 tcagcatttt ctaacccagg aatcatccgg aaaacacgtt tttgctcgcc ccatttcaag
    11341 tgagtctgaa agcccacgat gttgtataaa gaagctgcag cattgtcttg acgcagctgg
    11401 ataacagcat aaggacgttt gccagtttta ggatcttcca atccaactgg tttcataggt
    11461 ccgaaaagca tcgttttgaa tcctcgctgt gccattactt caattggcat acatccttca
    11521 aaatattttt ctttctcaaa agatttaagt tctgccactt cggcattgat tagtgcctca
    11581 tggaaagctt tgaattcctc ttctgtcatc ggacagttca agtaagctgc ttctcctttg
    11641 tcatagcggg acttcaaata tactttatcc atatcgatcg tagctttgtc tacgattggt
    11701 gcagctgcat catagaaata aaaaccatct gaaccattga atgctttgat gctttctgct
    11761 aatggatgag aagtcaaagg tcctgttgct actacggtga ttccttccgg tatttcagta
    11821 atttcttcgt ttatcactgt tactaatgga tgctctttga cttttcgggt aattgtttca
    11881 gaaaaagaat cgcggtcaac agctaaggca ccaccagctg gcactgctgt ttcatctgcc
    11941 gaagtgatta ctaccgaatt caatcggcgc atttcttctt tcaataaccc tactgcatta
    12001 gccaaactgt ttcctcttaa tgaattggaa catactagtt cagcaaaatt acttgtgtga
    12061 tgcgcctcag tcgatttgac tggacgcatt tcatataaac ggacagggac tcctgcttgt
    12121 gcgatctgcc aagcagcttc acttccggca agccctgctc caacaacatt taccattttc
    12181 aatgaaagaa cctcttttct attatttttg gatgtcttct tcgtaatctc catttacaca
    12241 gactacttgt ttaccgcctt tgactttctt ctcgaccaga tatccgtcac attttggaca
    12301 atttctgccc actggtttat cccaagaagt gaattcacat tctgggaaac gggaacaacc
    12361 atagaatagt ctgtttttct tagattttcg ttcaatgact tgtccttcat gacatactgg
    12421 gcaagtcaca ccgatttctt ttacgattgg tttcgtattc cgacaatcag gaaaattact
    12481 gcaagcataa aacttcccat atttccctaa ttttatcacc atcggatgtc cacagacatc
    12541 acaatcgaat cctgctggtt catctttgat ttgaattttt tcgatttttt cttccgcatt
    12601 cgtcagttct ttttcaaacg gtttatagaa acgatcaacc actttgaccc acgcttccgt
    12661 gccttcttca attttatcca aatctccttc catttctgct gtgaaatgga tatcgacgat
    12721 ttgagggaag aattcacaga ttaatgaatt gacaatttct cctagttcag tcggctcaaa
    12781 tcttttttgt gtcaatttta catagtagcg tctttggatc gtatcaagtg ttggtgcata
    12841 agtggaaggt cgccctaccc cgttttcttc tagtgcacga atcaacgtag cctcactaaa
    12901 acgtgctggt ggttgagtaa aatgttgttt tggttcaata tcaacagatt ggactacatc
    12961 gccttcttca agttctggaa gaatattttc tttctcttct ttgccgtcat ctctaccttc
    13021 cacatacact tgcatgaatc ctttgaattt gatttttgaa ccgttcgcaa tgaagatgac
    13081 accattttgt tctaatgtca ctttcattgt atctaaaata gctggtgtca tctgactagc
    13141 cacaaaacgt gaccagatca atgtataaag tttcaattga tctttatcta aatattgttt
    13201 gagttcatct ggtgtacgaa gcacactcgt tggacgcaca gcttcatggg catcttgtgc
    13261 gccttgagca tttttgatct ttcggtgacc atgtctagaa aattcttttc catacgtatc
    13321 ttcaataaat gctgcggcct ctgcttttgc tgtatcggca atccgggtag aatcagtacg
    13381 catgtatgta atcaagccga ctgttccttg tttacccaaa gtgattcctt catataattg
    13441 ctgtgctacc atcatggttt ttcttgttcg aaagtttaac ttacgggcag cttcttgttg
    13501 caggctactt gtagtgaaag gattcgcagg gtttcttttg cgctctttct tttctacttt
    13561 tttaacttcg aagtctgttc catcaattct agcagtgact tcttttacac tttgtgcatc
    13621 tggtaatttc tttttcttac cgtcgattcc ccagaaattt gctttgaatt tcttgcgagc
    13681 ttttttaaaa ttaccgtcga tgctccaata ttcttctgga acaaattctc ggatctcttt
    13741 ttcacggtca ataataattt ttaaagcgac agattggaca cgtcctgcac ttaacccttt
    13801 cttgacctta cgccagagaa taggactgat cgaataacca acgattctgt ctaaggtacg
    13861 acgagcttgc tgtgcatcta ctaaatctac atcgatcgtt cttggttcct taaaagctgc
    13921 tttgactgcg tcttttgtga tttcattgaa gaccacccga tttttttctt tcagatccaa
    13981 cccaagtagg tacgctaaat gccaagcaat cgcttctcct tctctatccg ggtcacttgc
    14041 caagtaaact ttttgagctt tttttgctgc ggctttcagg cttttgatga catcgccttt
    14101 tccgcgtata gaaatataat ggggttcata attgttttca aaatcgatac ccattttact
    14161 tttcggtaaa tctcttatat ggccaacact ggccattact ttatagtttc gtccaagata
    14221 tttctcaatt gtcttggctt ttgcaggtga ttctacaatc actaaatatt tatacgccat
    14281 tactgaatgg actcctttcc attgtcgtgt tcctactatt aaaagaggat tcatacaaat
    14341 cgtagctaat catatccatt cagaaaacgt ttgtcaagaa aagcaaatga aaagtatggt
    14401 gttattcttt aaattctgcc aagatatctt cacccgaaac cgtgcaaact gcaccgtctt
    14461 ggatcaaatg atgacatccg gtagaatgtt ctgttaaaat atttcctgga acagaaaaaa
    14521 ctgttcttcc aagatccaat gcttgctgtg ctgttatcag agagccgctt tttttcttgg
    14581 cttcaaagac acatgttcct ttagacaaag ctgcgataat ccgatttctc attggaaaac
    14641 aatgcttttt cgggccggat tgaggaggat attcactcaa aagaagatga tcatttgcaa
    14701 tcacctcttg caagtcacga ttttcagcag gatagacttg ctctatccct gttccaatga
    14761 ctgcgattgt ccgtccacca tgagagattg ctgcttggtg accgtatgta tctatccctt
    14821 tcgcgcagcc actgacgatc acatatccag cttctatcac tggttttagt aaataataaa
    14881 ctgttttttt ggctaaagca gaagtgtgtc ttccaccaac aacgcttaac atgttacaag
    14941 aaagtaaaga tagatcacca ttataaaaca gtacaattgg cggataggtc aagtgaagca
    15001 gctgaggggg atatttggga tcaaagcaag tgataaacga ctgtttcgat tggattttgc
    15061 cccagttagc gtgaatttcc tgccaacttt tgcgaaacag ctcccggtgt ttggttattt
    15121 tacctatctt aatgatgtct tcaaatccga tttctttata aggatactgc atcatccagt
    15181 tagcaagtgc ccattttcct tgactactga ttccttttgt ataagcaagt ttcaataaat
    15241 tttcttctat ttgatacatc ataaaaccgc ctttcgtctc tctttctaat aagatacgta
    15301 aaagcggctg ttttttattg taaatatttt tttacaggtg caaaactttt acgatggatt
    15361 ggcgtaattc cttgtcgttc aagtccttct aagtgttcct ttgttccata acctgcatta
    15421 tgcgagaaac catatccagg ataaagcacg tcatattctt tcatcaattg gtctcgatat
    15481 actttagcaa tgatacttgc agcagcaatc gacacagatc gtgcatcccc tttgattatt
    15541 ttttcctgct gaataggcaa atccaaggtc atcgcatcga tcaacaaata gcttggtaca
    15601 atggacaatt tttctactgc ttggatcatc gctaatttac ttgcttcata aatattcaca
    15661 tcatcgattg tctgctgatc aacgatcccg atcccgattg ccagggcttt ttcttgaact
    15721 tgttgataaa gaagttctcg ctttttctct gataattgtt tggagtcatt cagccctaat
    15781 atgatctcat ttttcggcaa gataactgca gctgccacga caggtccagc taatggtcct
    15841 cttcctactt catcaatgcc ggcaatccat tggtgacctt gtgcgtaccc ttgcttttca
    15901 aagcaaaaca tttcttcata tttttcctta attaatgctt cctttttcaa tcgtcgttca
    15961 aaagaagcca gtgcttgctg cacgcctttg cgtggatcat ttcttaatga ttctagtgta
    16021 ggatcatcta ttctctgtat gcttttcaat cgttctttga tactttggat ggattcagtc
    16081 atcacttttt tgctccattt cttcgcaacg atccagtgtg tatcgtccaa gttttccttg
    16141 acgaatatca tgcacgacta attcgctgcc acgatcatag tcttctttat acccacgttt
    16201 tgctgtaata tccatcagta attccggtgc caatttttct tcttgctcga ttgataaacc
    16261 ataacgatcg ctgattcttc ctgaataata tcgggcaaaa aagtctaatc cgtaaatcgc
    16321 taaatcatct aaatggacaa gctggtcttt gatcgcgcct gtcagcgcta gttttttgcc
    16381 gatttcttca tcttcaaatt tgggccataa aatccctggt gtgtctaaca attccaactc
    16441 attacctaaa cgaagccact gctgaccttt tgttacgcca ggcttatttc ctgtctgtgc
    16501 aattttcttg cctactagtc gattcatcaa cgtagatttc ccaacatttg gaatacctaa
    16561 gaccattgcc cgaatagcgc gaggcttgat cccccgagca gcatctcttg cccgtttttc
    16621 tttcaatgct tctttagccg caggtactag acctttaatt cctttacttt cttgtgccgt
    16681 gatagctaat gcaagatggc cttgtttacg aaaatattct tgccacagct ttgtctgttg
    16741 tggatctgcc agatccgctt tattcaataa aacaaggcga ggtttttgct gaatgatctg
    16801 atctagcatt ggattacgag aagataaagg taatcgtgcg tctaccaatt caaatacgat
    16861 gtccacatat ttcatttttt cagaaacttc tcgtcgggct tttgccatat gtcccggaaa
    16921 ccattggatg gtcattgttc tctccattcc tttcataccg gaatattccc cggctttact
    16981 tgtttttttc tttttcaata taaattcact tttttatt
//
LOCUS       DAZKSU010000056        16941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000056 DAZKSU010000000
VERSION     DAZKSU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.056"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(258..1763)
                     /locus_tag="ACG8Z3_001930"
     CDS             complement(258..1763)
                     /locus_tag="ACG8Z3_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0824237.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1760..2446)
                     /locus_tag="ACG8Z3_001931"
     CDS             complement(1760..2446)
                     /locus_tag="ACG8Z3_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0824238.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="ACG8Z3_001932"
     CDS             complement(2639..4060)
                     /gene="gndA"
                     /locus_tag="ACG8Z3_001932"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0824239.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="ACG8Z3_001933"
     CDS             complement(4230..4409)
                     /gene="rpmF"
                     /locus_tag="ACG8Z3_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0824240.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4467..5036)
                     /locus_tag="ACG8Z3_001934"
     CDS             complement(4467..5036)
                     /locus_tag="ACG8Z3_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0824241.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5222..6124
                     /locus_tag="ACG8Z3_001935"
     CDS             5222..6124
                     /locus_tag="ACG8Z3_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0824242.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="ACG8Z3_001936"
     CDS             complement(6159..7943)
                     /gene="pyk"
                     /locus_tag="ACG8Z3_001936"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0824243.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="ACG8Z3_001937"
     CDS             complement(8017..8979)
                     /gene="pfkA"
                     /locus_tag="ACG8Z3_001937"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0824244.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="ACG8Z3_001938"
     CDS             complement(9147..12461)
                     /gene="dnaE"
                     /locus_tag="ACG8Z3_001938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0824245.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12692..12877
                     /locus_tag="ACG8Z3_001939"
     CDS             12692..12877
                     /locus_tag="ACG8Z3_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0824246.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12939..14135)
                     /locus_tag="ACG8Z3_001940"
     CDS             complement(12939..14135)
                     /locus_tag="ACG8Z3_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0824247.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14305..15741)
                     /locus_tag="ACG8Z3_001941"
     CDS             complement(14305..15741)
                     /locus_tag="ACG8Z3_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0824248.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16034..16843
                     /locus_tag="ACG8Z3_001942"
     CDS             16034..16843
                     /locus_tag="ACG8Z3_001942"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0824249.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4968 a         3550 c         2961 g         5462 t
ORIGIN      
        1 aaataataat atagcaatct ctttataaaa ttgcaataga aaattcccta tttttattta
       61 ttatttttaa acacgaatat tacagcattt tattgttttt tcgttcatct aaaagatact
      121 tacatttaat taacggatgt tattctattt aattgttcgc tagttttttt ataatgacat
      181 ccctgaattt ctcacttgct tgtattttgt accttattct ttgttggata tataaaaaaa
      241 caagtaaagc acatgcttta cttgtcttct gcctttgcga taggaataaa gattctaaaa
      301 attgtccctt gccctactac actttctgct aagatacgtc ctttatagtt ttcaaccagt
      361 tgtttagcga tagacaaccc cagaccattt ccacccttcg ttctagctct tgctttatcc
      421 actcgataaa aacgattgaa gatcttatta aggtcttctt tgggtattcc ttcgccaaag
      481 tcctgaatcg cgatctcaaa ttcattcaga gtggaagaaa tagagatatg gacttctttt
      541 cgtgtagttg aatactttac tgcgttatcc aatatgataa tgattaattg ttcaaagtga
      601 ttacgataaa tttgcagggt cacttctttt gtcaaatcat catctaaagt gatcacatag
      661 tctggatata atatcttgaa attattataa acttgatagg tgacttcttt tcctttagtt
      721 gtttcattcg cataatgaac atctacttgt tctgctcttg ataagtctag catctcttga
      781 actaagcttt tcatacggct gatttcttgc atgctggcgc ttaatgattc ttctaatatc
      841 tcaggatcat ctttccccca acgatttagc agactaagat gcccttcaat aattgcaaca
      901 ggtgtcctta actcatgaga tacgtcttcc acgaattgct cttgttgttc aatatacaaa
      961 cgcatacggt caagcatctc attgaatatt tcagctaaat ctgccaattc atcatttgtg
     1021 tcgatttcag gcatgtggat atctgattgc ggatctttac gtatagtatc cattgtatct
     1081 ctgagtacct tgagtggttt taagaaataa gaagataaaa agaaacctaa aatactactt
     1141 aagatcaaag aaacaacttc taaaacaata agagtcaata atagcttatt tcgaatatca
     1201 tagaaagaag ataattcata gaagctttgc gcatagccga tcttttctct tgtaccttta
     1261 gaatatatag gctggataga taaaaagcca gtttttcctt ctagtgtaac gatcgttgga
     1321 actttacgtg tagtctgaat caggtttcga ctgttttcat gggttttaaa aatcaagttc
     1381 tcgtccaaat cataaacata aagatccatt gatggctgcc ctagctcagc tataaaggaa
     1441 tcgatcttca ttaagtttcc ttcgaccagt gaagtatgat ctacgacctc atctccgtca
     1501 tttgtcgctt tcgtcagatt acgatacgta tttactaatg tcagttcttc atccgagtta
     1561 gccaaacgag aagctacttc tgaaatcgta cgctctacat tgtttctttc ttttgccaca
     1621 atcagattga tagatgattt ataggtaatg acagcgaaaa ttgtaaacac aacgaatatg
     1681 aagaaagaac tcgcaaaggc ccacttgatt gtcaaggagg gccctttcag ttctcttttt
     1741 tccgattttt tcggctttat cacgagcgca tcacataacc tgttccgcgt acagtttgga
     1801 tatagctttc ctccccggga acgtcaattt tgttgcgaag ataacgaata tatacgtcca
     1861 ctacgtttgt ttcaacttct gtttcgtatc cccacacttt atttagtaaa acgtcacgtg
     1921 ctaataccac gttcacgttt tccattaaag taagcagcaa ctcgtattct ctttttgtta
     1981 gttcaattac ttcatttccg cgacgtacga cacggttttc tttttcgata gtcaaatcgc
     2041 gataagtaat agtagtttgt ttagccacat ttttatcgcc ttcaatatcg attcgacgca
     2101 acaatgcacg aagacgtgct aataattctt cgatagcaaa tggtttgacg atgtagtcat
     2161 ctgctccgtg gtcaagtcca gatacacgat cgatcacaga atctcttgca gtcatcataa
     2221 tgattggtgt atttttgact tggcgtacac gtctgcagac ttctaaaccg ttcaattctg
     2281 ggagcatcag atccaacaat atggcatccc actcattatt taatgccgca tctaaaccag
     2341 ttcggccgtt gtagtggact tctgcgttat acccttcatg cttcaactct agctcgacaa
     2401 atcgtgccaa gttcttttca tcttcaatga ttagaatatt gctcatttat tattttatcc
     2461 tttctattct actggctttt taaaaactct atcattatac cgataatatg cttaaaaatc
     2521 tatagtcatt ttattatgga tagcaatttt aaaaaagagc tgaagtctac tcataacaat
     2581 cgacttcagc tctttcgtcc tctttttatt ttctcatttc ctaacagccg aaagactatt
     2641 attcttcatg gtaccatgag taatggaaga ttccttcttt gtcgacacgc tcataggtat
     2701 gtgcgccaaa gtaatcgcgt tgggcttgga ttatatttgc aggcagacgt tctgctctgt
     2761 atgaatcgaa gtaagcgatg gctgatgaga aagtcggtac aggtacgcca gcttggacag
     2821 ccaaggcaac gacgtctctg actgattgct gatatttttt cgtgatttca acgaagtaat
     2881 catctagcaa taaattttcg atctcaggat ttttgtcata ggcatccgta attttttgca
     2941 agaatcgagc tcggatgata catccagcgc gccagatttt tgcgatttca ccaaatggca
     3001 agtcccaatc aaattctttt gaagcgacac gtaattgagc aaatccttgg gcataactca
     3061 tgattttact gaaatacaac gcttctcgga ttttttcgac caattctttt ttgtctcccg
     3121 tgaactcaaa atcgttcgta cgagacaaaa tcttgcttgc ttgtacgcgt tcttctttat
     3181 aagcagaaat atagcgggca aatacagatt cagtgatcaa tggaagtggc acacctaagt
     3241 caagcgcgct ttggcttgtc catttacctg ttcctttatt cccagctgca tcaaggatca
     3301 catctactac tggtttacct gttccttcat cgtctttacg tgtcagaatg tcagcagtga
     3361 tttctatcaa atagctgtct aattcgcctt gattccattc tttaaagata tcagccattt
     3421 cttctactga aaggtttaaa atattcttca ttaagtcata agattcagca atcagttgca
     3481 tatctccata ctcaataccg ttgtgtacca ttttcacata atgtccggct ccatttgggc
     3541 caatatatgt gacacatggt gctccgtctt ctgcttttgc agaaatttgc tctaagattg
     3601 gtgcaactag cgcataggct tctttttgtc caccaggcat gattgaaggt ccttttaaag
     3661 ctccttcttc accaccggaa acgcctgtac cgataaagtt gatgccagag ttggctaact
     3721 cttcattacg acgcatcgta tctttaaaga atgtatttcc tccgtcaatc aaaatatcgc
     3781 ctttatcaag gtgaggaagt aactcttgga ttgttgcatc tgttgccggt cctgctttga
     3841 ccattagcaa gatccgacgt ggtttctcga tagattctac aaattcttcg attgtatatg
     3901 ttgctttgaa atttttatct gggtgttctt ctactacagc ggttgtttta gcccctgtac
     3961 gattgaataa agccactgaa tagccgcggc tttctatgtt caaggctaga tttttaccca
     4021 taacggccat accgacaacg ccgatttgtt gttttgacat atgaactcct cctacatacg
     4081 ggatttctcc ctaatctctt acattatagc ttaacacggc tacattgcaa actaaagaac
     4141 aacatatttt ttatttaccg actacaaaaa aataaacctc aaggctccat ggttagcctt
     4201 gaggttttac attttctaat taaaatgtat taagcttctt ttgacattac gtccttacca
     4261 tcatagtaac cacaatttgc acatacgtga tggctttttt tcatttcacc acagtttgga
     4321 cattcgttta accctttgat tgtcaatttg tagtgtgtac gacgttttgc ttttttagct
     4381 tttgatgttc ttctagctgg tactgccatt ttgctacacc tccttgtaaa gtcttgtatc
     4441 taaattagac acaatatatt ccaatcttac ttgttatcgt cgtcttttga tttagaatca
     4501 aaaagttcag atagttttgc aagacgagga tcaattttat tctcggcttc ttccattttt
     4561 ctgcgctggt attcttcttc ggaaacaact tcccaatcat tgccttttgg aagttctgtt
     4621 gctgcttcct cttcttttgt caatacccgc attggaatcg ccaataagat gttgtcttca
     4681 atcgactctg ctaaattcaa taatggacct tcaattagca aaatctcatc tgcagacatt
     4741 tgttcatctc tactttggaa ttgttcttct gtcatgaata cttcgtctac agaaaaatcc
     4801 atgggcagtt caacaggttc tagtgaacgt gttgaaggaa ctgtcaaagt cgtctgcaca
     4861 gtgtaatgaa cgatatagcc tttcttatca actgagacca aaccttcgac ttccactggc
     4921 gcaacatcaa gtattaaatt gtctcgcttc atgagagcgg ctttcaagtc gagcgtctca
     4981 tgaaaagtta acggtgtctc ctggtatttt ctcaattcca ataaagacca tttcattttt
     5041 catcactcct aagcaacaaa agcaattata cagggcaaaa agcatgttgt caatgaaatt
     5101 ttcttgacac cttgcagttc ataaaaaaac agaaaaatct ttattagaaa gcctctgttt
     5161 gctatcatcc tcagaatcat ttacaataat cattattcca ttaaaggagc ggatgatctt
     5221 catgaacacg aaagatatcg aatactatgt ccaaatcgtc aaacaaaaaa attttacgaa
     5281 agtggctaat tatttccgtg tgagccaacc tactattaca tatgcattac aacgtcttga
     5341 aaaagagttt gacaccgtac ttattacaag agatcgatcg catcacgaat tgatcgtcac
     5401 gccttctgga aagcagctgt ttctccatgc acaaaccatt ctccaagaga tttctactgc
     5461 aagagctgaa atcactcagt tgcaaaagaa aaagcttcgt tttggcatgc ctcctattat
     5521 cggcaatgaa tactttccta aactgtcaag ttactttatc aagcatgaat tgatgaatca
     5581 aattgagatt accgatggag gctctcggga cttatacggg atgatccgac aagggaggat
     5641 cgatttagct gtattaggct caacacagcc aatcgtggat caatatttag aaacagagtt
     5701 acttttggac aaacgtttca tgatcgtcgt gccacctaca catcctcttt ccaagcagga
     5761 tactgtctct ttcaaagata tagcagatga acagtttgta ctattgaacg aacattatgt
     5821 ccaccccgca gcattcacta agctttctaa acaaacacat actcatccaa aagttgttta
     5881 tcaaagcaat gacttgacga ttttaaagag tatggtccga gaaaatgtgg gtatcggttt
     5941 tttggcagag attgctgtcc atcctgaaga tcatttagtt tgcctccctc tttcagatga
     6001 agttcagcct cgcttcttga tttcattggc aaaacgaaca cagcagatcg tcacgcctct
     6061 tcaagaacaa gtagtccaag ttatccatga attcgttaca gcacaaaaaa acagctcttc
     6121 gtaaaagaag agctgttttt attttttatt aagctaaatt agatagctgt tgtttcgcca
     6181 cggtaaacga tgccgcgacg tgaatcaacg gtaatcaatt caccttctgt aattgttgtt
     6241 gttgcgcctt tagcaccaac gattacagga atatcttgtg caattcctac aactgcagcg
     6301 tgagaagtca atccgccttc ttcaacgata acagcagctg ctttttcgat tgcaggcata
     6361 tattctttgt cagttgatgg tacaactaga accattccgt cttttgcttt ttttacagct
     6421 tcttcagcag atgaagcaat aactgcattt gcaacgacag atttttgtcc aacgccttga
     6481 gcttctaata atttagaacc aatcatttga attttcatca cgttagttgt tccgcgttca
     6541 ccaactggta caccagcagt gatcaagatt aaatcgcctt ctgatgcgaa acctaattca
     6601 acagcttttt tagctgctag ttcaaacatt tcatcagttg ttgatggttt ttcagcaact
     6661 gttgggtaaa cgccccagtt taacatcaaa ccacgttttg tgcgttcgtc aaaagtaaca
     6721 gccaagatat ctgcatctgg acgatatttt gaaatcattt ttgcagtgta tcctgattca
     6781 gttgcagcaa cgattgtttt cacgcctaga ttttttgcag cacgagctac agataaaccg
     6841 attgtttcag ttacatctgt tttatcgaat tcgttcaatt ggaatgtacc taattctgat
     6901 aatgcatttt cagcttcttg gtcgatacgt gccattgttg aaacagcttc aactgggtag
     6961 tccccgtttg cagattcacc agatagcatt gttgcatctg tgccatcaaa tacagcgtta
     7021 gcaacgtcag aagcttccgc acgtgttgga cgtgggtttt cttgcattga ttctaacatt
     7081 tgtgtagcag tgataactgg gataccagca gcattacatt ttttgataat gcgtttttga
     7141 accattggta ctagttcagc tgggatttca acacccatat ctccgcgggc aatcataaca
     7201 ccgtcagaaa ctttgatgat ttcgtcaatg ttgtcgatac cttcttgaga ttcgatttta
     7261 gggaagattt gaacatgagt catgtctttt tcttctaaga tttcgcggat atctagaacg
     7321 tcttgtgctt tacgtacaaa acttgcagcg ataaagtcga tatcgttgtc taaaccaaaa
     7381 cgaatatcgt cagcatcttt tggagtaata cctggcaaac taatagaaac gcctggtgca
     7441 tttacccctt tacgtgatcc tagcatacct gcatttttaa cttctacgac taattcacgg
     7501 tttgcttcgt ctttttcaat gatttgcata tcgatcaaac catcatcaaa taaaatgtgt
     7561 ccgccaacgt gaacatcgtc aaataatcct gggtaagtaa ccgcgatttt ttcttttgaa
     7621 cctacatgtt caggatccat agagatgcgt gtttgatctc ctacttcaaa tttgatgtat
     7681 cctgcacgac caaaatcagc ttctgttgtg ccttgaacag ttgtacggat ttcagctcct
     7741 tttgtatcta aaagaatacc gacatctttt cctgtctttt taactgcttc acggaccata
     7801 gtcatacgtg ataattgttc ttcatggtca ccatgagaaa aattaaagcg gaagacattt
     7861 gccccagatt caatcaattg agaaataatt tctactgagt tacttgctgg tcctaatgta
     7921 ctaacgattt tggttttttt catttaaaaa actctcccat tcaattttgt ttatttaaag
     7981 ctgtacacag cttaaatagc ttggtttctt tcactcttaa aatgaaagtg aattatttag
     8041 atcatatagt gataagtctg gtttatgttt gttgttttca agtgtatcaa tgatgtcaga
     8101 cgcaacgatt tcgttgtcat ggataccgac acataatcca ccttttcctt cttgaagcaa
     8161 ttcgacagcg taagcaccga atttacttgc caacacgcgg tcgcgtgcgc ttggtgctcc
     8221 accacggaca acgtgtccca agatagatac acgtgtatga tagtcaccaa attctgaaag
     8281 tttctcagcg aattcatttc cgcccattac accttcagcc aagatgatca agcaatgttt
     8341 cttcccgcga tcgcggcctt cttggatttt tttagcaact gaagccatat caaaatcatg
     8401 ttctgggatg atgatttcat ccgctccgcc agccacacct gaccataaag cgatgtctcc
     8461 tgcattgcgt cccattactt cgataacgaa tgtacgaaca tgagaagtcg ctgtgtcacg
     8521 aatacggtca attgattcta gtactgtatt gattgctgta tcaaaaccga ttgtgaaatc
     8581 agtcccagga atatcattat cgattgttcc aggaatacct actgctggga aaccgcgttt
     8641 tgtcaaagcc attgcgccat gataagagcc gtctcctccg ataaccacta gaccttcgat
     8701 tccgaatttt ttcaattgtt cgattccttt taattgacct tcttcagttg caaattctgg
     8761 ataacgtgca gaatacaaga atgtaccacc acgttggatc ttgtcaccta catctgccac
     8821 atcaagacga cgaatatctc cagcaactaa accggcaaaa ccataattga tcccgtatac
     8881 ttccagtcct tcgtaaatac ctttacgaac aacggcacga acagctgcat tcattccagg
     8941 tgcgtctccg ccactggtca aaattccgat gcgtttcata ataaacattc acctcattaa
     9001 aatactttat cctaagtatg acttaggttt acaaccattt ttgcgttaac cacgactaac
     9061 attctaacat tatttttctc acttgtcttg atttacggat aaaaaaatga aaaaaaatga
     9121 aagcaagtag tattttttac cgtaagttat ttgaaaacca cattatcttc gccaagcaga
     9181 taaataagtt gccgctttat actcttgctg tcatccaccc aataagcatc tgacagaact
     9241 acttttcgtt gcgttttttc gtgatacata actactggta tatatcctgg gtgtttctga
     9301 aaaatcactt gtaattgttt cattatctcg ggatcttccc gttcttctgt aatacgcaaa
     9361 tagcaagttt gtgaaggata ctgggcttct aactggttag gatcagcaaa gctttctcca
     9421 attagttgca gttcctgatt atatttgctg atttcgctcc gcccttgaac aaagatcact
     9481 cgatcaacgg caaacaaatt acgatattta cgataaagag ttggaaaaac tgtgacagaa
     9541 atttctccac tactgtcggt tccttcaaca aaggccatca gttctccttt ttttgtccga
     9601 atttctctaa cttccttgat catcactaat aaattgactg gttgattagg gacgatatct
     9661 gtaatcagct gaacttgttt agctaaacga agacgatcaa acccttctgc aggatgacct
     9721 gacaaataaa tacctaagta ctcctcttcc agcttcaagc gttcttctag tgtatagtca
     9781 gcgacttctt cttcttttaa tgccatgatt cccagcaagt caagacttcc accactgtaa
     9841 acgatatttt ggatttttcc ttcgagatca agcatcaatt gtttcctgtt aggagataat
     9901 tcatcaaaaa ctcctactgc tgtcagtgat tgcagtaact ctaatttcag ccaacgctta
     9961 tcgatccgta tgaggaattg atccactgac tggaacgggc cattttgttt tctttcgttt
    10021 aatatatctt ctacaaaatc acgccgaata ccttttatcg ctgtcagtcc gaatctgatt
    10081 tcttccttat tgaccaactc aaaactggaa ttactttgat taatggacgg cgaggaaagt
    10141 ttgagtttat ttttacgtgc ttctgcaata tattctttca attttgatgt gttatgccga
    10201 acagaattca tcaggctggt aaagaaagca cctggatgat gggcttttag atacgccatt
    10261 tggaatccca cgaatgaata agcaaatgca tgtgaccgat tgaatccata gtttgcaaat
    10321 ttttcgatat attcatatac ggtcgctgct gttttttccg aatatccctg ttgtaatgct
    10381 cctgatacaa aatgcgactt ttcttcatct aatacgtctt ttttcttttt actcactgcc
    10441 cgtcgcagaa tatctgactg ccctaatgta aatccagcca tagtcgaggc gatctgcatg
    10501 atttgttcct gatagacgat cactccatat gtgtttttca aaatcgggat cagtgaatca
    10561 tctgggtagc gaatggcttc ttttcctttc ttcctggcaa tgaaggtatc tatattttgc
    10621 atagggcctg gtcgatacaa tgcattgact gcggcaatat cttcgatgtt ttctggatct
    10681 aatcttcgta aaacgttccg gataccagca gattcaaatt gaaatactcc tgaggtttcg
    10741 cctcgttgaa acaagagcag cgtttttgca tcatttagcg ggatctcttt taatcggatc
    10801 tcataccctt ctactcgctt aacagctttc aaagtaaaat caataatcga taagttgcgt
    10861 agtcctaaaa agtccatttt caaaagaccg attgcttcta cctcattcat cgtatactgg
    10921 gtcagaaaag cttcatcaga accgcgttgg agcggaacta gttcaagtag attcttatcg
    10981 ctgataacta cacctgctgc gtgagtcgaa acatgtcttg gcaatccttc taaaatcact
    11041 gcagttttga ataaaagttg gtttttttcg cttgcctgaa ctaattctac taattgatgc
    11101 gaagttttat aagcttgggt caatgtgatt ttcaattgat tgggtattgc ttttgaccag
    11161 cggtttgctt cactttgcga taaaccaaac actctagctg catctctaag aaccattttt
    11221 gctgccattg tgccaaatgt tgcgatttgt gccatatgat attgtccata cttttctcga
    11281 acataattca atacttcttc acgtcgattg tcaggaatat ccaaatcgat atctggcatc
    11341 gtaaagcgtt cggggtttaa aaagcgttcg aacagcaaat catattctaa tggatccaca
    11401 tctgtgattt ctaacacata ggctactaat gaacccgcag cagaaccacg acctgcaccg
    11461 gtaacgattt tgtttctatg agcaaaagcc attacatccc atacgatcaa gaaataatca
    11521 tcgaatccca tttgatggat gacagataac tcgtagtcca aacgttcttg gtattccttt
    11581 gtatagtcag tgattcggcg ttccacttgc gaatagcata actctcttaa atattctcca
    11641 gcttgttttc cattaggaac tggataatgc ggaagtaatt tctgatggaa ctggaattca
    11701 aatgaaatcg attcagctaa acgctgggcg cctattacag cttgggaaag acccgctttt
    11761 tcgaatcgtt tgatcatttc ttgttctgaa agaagataga catttgctgt tagtggtaaa
    11821 gatgcagata aattttctaa aggtatttgg cttccctctt tgatatgttt catcacttca
    11881 agtgcaaaaa gttcttcttg ttgtaaacta gcgatgggat gtaatgcaca aagcgaataa
    11941 ttttctttta tcacatactg ataccattca ggcaattgat caggcgtgtt ttctgtcaag
    12001 ccgatataaa aatttctagt aaaaacattt tcaagtaaaa actgccaatg accgaaaaag
    12061 ttatcctctt tattgaaagc ctcagaaaca gaatgatttg ccggaacaac aaacgctaga
    12121 tggtcggttt ctgtcatttc tgcaagttcc aatttttctt ggatcatttt tcggctggaa
    12181 agtttcatca gctgctgata acctatttca tcttttgcgt acgcaagcaa gccataattt
    12241 ttttcttcat aagtataact aagatgtaat ccaatgattg gctgtactcc ttcttctaca
    12301 caaatacgat agaattcagg tgcaccgtat aagacatctt tatccgttat ggctaatgaa
    12361 gtgtagcctt gtttttttgc ttcttgtaca tattctcgaa tacgtatcgt actttgtaaa
    12421 agagaataag aagtaattgt ataaagttgt gggaaaaaca tcttgcattc cgcctttttt
    12481 ttcgttaaaa ttcactagcg ttaaaaggtt tgatcctttt actttcatta agatagcagg
    12541 cgctgaagtt gattattcca cttcatttgc ttgcttgtaa tctgtaggaa tgagttcttt
    12601 cgcaagaact caaattttat tgagatttgg tctcgtttct ttacattcaa agaaaatctg
    12661 gtaaaattaa gatagaaaag aggtgttctt tatgcgatat atcgttacat tattctgggc
    12721 tgtcgttcta ggtcaagtag tcggttatat tggtgctgca ttaacaagcg gaacatatga
    12781 ttttactttg acaacaatca tttcattcat tgcgggtgtc attatccttt tgattggtgc
    12841 agttgcgcca agaaaagaaa cctctgccca ttcgtaaaaa aacagccaag agcttgagat
    12901 ttctcaagct cttttttatt ttctactcct caccaccatt ataatctgct gcgaagaact
    12961 ttacgaaagt tgaataaaga aagaagaata tttgacagaa caaacaaact tgtcacaata
    13021 aaaacagcac tgtaattata agcatgcgcg actgtcgatc ctagcatggg acctaaaact
    13081 tgtccaaagt tgctaaacat ctgattgtag ctatagattc ggctgactcc ttcttgaggc
    13141 gtaatcttac tgatcagtgt attgacagaa ggcatcaaag cgccagttga aaagcctaaa
    13201 ataaagcgaa gtatacctaa ttggagcggt gtagtgacga atgccatagg taaataacag
    13261 caaaatgata aaatgagtcc tccaattaga actttttgat tcccaatctt atcccctagt
    13321 tttcctagtt tcggtgaaga aaagacagcg gaaacaccag ctatcgaaac aatcaagccg
    13381 ctgacaaaaa ggatattttc tgtacttccg ctcaattcac gaatatataa ggtcaaaata
    13441 gggctgatcg tagtgattcc tatctgtaaa atcaaggtcg taatgaagag accgatcaaa
    13501 atagagagat gatccatttt tgaaaaaatt tctttggtgc ttatcagatc ttttttctcg
    13561 attggatgga aatcttcttt gactaagaaa atggtcaata ccgttgtgat catcaacaaa
    13621 gcccctgtga tcaagaatac attttccata ccaaaccact gagccagtgc accgcccatt
    13681 gaaggtccga tcagatttcc agcaatcgct ccggtagaca atgttcctaa cgcccatccg
    13741 ctcttttctc taggtgcctg tgaagcaatc atcgctgtgg catttggaat atatccagac
    13801 aaaatcccat tccaaaaccg catgatcaaa agccaatacg cgtttggtac aaaagccaaa
    13861 gatcccattg ttaatgtcat ccctgctgct gcacggatca tcatgagttt tcggcctttt
    13921 cgatcagcta gatttcccca gattggtgca accacggcag atgcaaaagc agtcacagag
    13981 attgctagtc ccgaaaacaa ttcgatttga tcttttggtg ttcctagttg ctcaacataa
    14041 actggtatga atggcataac taaactaata cttgcaccag tgaaaaaaca gccaatccac
    14101 gcaatcacta aattttttct ccaattaatc tccatctatc atccctcttt cgtctctttt
    14161 atcataccta ttttcgaata aacaagcaac gatctcgcca gtaaaaagag aaaaatcccg
    14221 caaaggtcat tttatatgta acgttctttt tatgtaaaac aaaaagagat cagaacaaat
    14281 actgttccaa tcccttctct ttagttaaat tgttgctaga atgatgaagt tgataatgaa
    14341 taaaatatca acgatccaca aagcaacaga aacttgattg tattttcctt tgattacttt
    14401 cacaattgta tagaaaataa atcctgctgc aatcccatag gaaatgctat agcacaatcc
    14461 cataaagatc gatgcaaaga aggccggtaa agcttcttcc atatctagcc atttgatgtc
    14521 tgcaaaagaa gccatcatca ttactccaac taaaatcaat gctggcgcag ttgcttgatt
    14581 tggtacaatg gcgatcagtg gagagaataa actacttaat gcgaatagca ctgccactac
    14641 tactgaagtc aaaccagtac gtccaccggc cccgatccca gctgcagatt cgacataagt
    14701 tgttgtattc gatgtaccaa agatagcccc cacagaagta gcgatcgcat cggcaaatag
    14761 tgctttatcc atttttgttt tgaacccacg accatcttcc aacgcgatct cgtcttcttt
    14821 tgagaaaata cctgtgcggc gtcctgtacc gataaatgtt ccgatcgtat cgaaagtatc
    14881 agataaactg aatgcgatga tcgtcatcaa tacttgagga atcttggaag aatcactgaa
    14941 taaagattgc atgccttcag aaccaaatgc tgcaccgaaa gttgttccta attctttaaa
    15001 tgaattacct aacgaattcg tttgccaatc aatggaactt aaatcaacaa cacccataaa
    15061 aataccaagg actgtggtcg ctacgatacc gataagaata gccccacgga cattttttac
    15121 aactaaaatg gctgtcagta ccaaaccaat cacagccaat aagataggag cgttgtcaaa
    15181 gttggctaac gcagggacga ttccaccatt tatcgtaaca tttgtcgctt ttccgccttc
    15241 aacgacagag ctcgaaatag ctgactggtc tgctgaaaaa gataagaatc ctgcattttt
    15301 gattccgaca tacgcaacga aaataccgat cccgccacca atcgcatgtt gaagactttc
    15361 gggaatcgcc cgaataatca tttttcgaat atttgtgaca gtaataaaaa tattgattaa
    15421 tccgcaaata aacaccattg ccaaggcttg ttgccaagaa taaccaagcc caaaaacgac
    15481 cgtaaaagtg aaaaatgcgt tcaatcccat accaggtgcc tgagcatatg gtacatttgc
    15541 gaacaatccc attaccagcg taccaataat cgatgcaatg attgtcgcta gaaatactgc
    15601 ttggaagggc atacctgatg ctgacaaaat cgtcggattt acgaataaga tataactcat
    15661 ggcaaagaat gtcgttactc ccgccattat ttcagttgat acatttgtat tgtttttctt
    15721 taaaccgaaa aacttttcca taaaaagttc cactccctga atttttttct gatggaaaac
    15781 tataataata aaaaataagt aaaatagggc aaatccatcg aacaatttca aattatatag
    15841 acgtttcatt cgcttttcaa cgttttttta acaaaaaaag cgaaaataat acttaacgtt
    15901 cgtgtttagc ttatgtttag ctatttatat agccataact gtagtttttg ttaggaatag
    15961 ctgattgctc ttgttaatta cctaaaatat gctagactaa aacaaatgct agttgaggaa
    16021 ggaaggaccc ttattgaata aaaaattaat tgccatcgat ctggacggaa ctaccctcaa
    16081 tcaagattcg atgattacat ctaaaacaaa agagactcta aaaaaagcaa tcaatgccgg
    16141 tcatcatgtc agtattgcta caggacgccc attccgaatg agccatcagt tttaccaaca
    16201 attagaatta acaacaccga tggttaattt taacggcgca ttagtacaca tccctaatca
    16261 gcactgggat ggtgaaagag aaacgttgat caatcgtgaa atcgcttttg aaatcttatc
    16321 gcaaaaaaag cagctaaatt tagattttat cgctgctgaa aatcgtgata ctttttttat
    16381 tgacagtttt gatttctttg atgaaaaaat ttttgcttct tctcgtccag gagagaagaa
    16441 tttgctttct cctaaaaatt tgacgacaaa tcctacatct ttgcttgtcc gaacagacaa
    16501 acgttttgca ggagctgtat ctgctgaatt aacacgtcaa tttggcagtt atgtcgatgt
    16561 tcggacatgg ggtggaccta ctgccatctt agagattgtt tcaaaaggaa tccaaaaagc
    16621 aaaaggcgtg caagagattg ctaactacct gtctatcgat caaaaagacg tcattgcctt
    16681 tggtgacgag cataatgatt tggaattgtt ggattatgca ggttggggag tagctatggc
    16741 taacggtact gaccaactca aaggcattgc aaatgatgtc actcctttga gcaatcagga
    16801 agacggttta gctgtttatc tggaaaaact acttaaacta taatagagaa ttagattcca
    16861 caaaaaagag gttgtgacaa tttttgggct ctataattta tgggactgat gaatcagaat
    16921 tgattcttca gccccatttt c
//
LOCUS       DAZKSU010000057        16890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000057 DAZKSU010000000
VERSION     DAZKSU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.057"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1086
                     /locus_tag="ACG8Z3_001943"
     CDS             <1..1086
                     /locus_tag="ACG8Z3_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0824250.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1304..2446)
                     /locus_tag="ACG8Z3_001944"
     CDS             complement(1304..2446)
                     /locus_tag="ACG8Z3_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0824251.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2451..2636)
                     /locus_tag="ACG8Z3_001945"
     CDS             complement(2451..2636)
                     /locus_tag="ACG8Z3_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0824252.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2990..3235)
                     /locus_tag="ACG8Z3_001946"
     CDS             complement(2990..3235)
                     /locus_tag="ACG8Z3_001946"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824253.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3229..3630)
                     /locus_tag="ACG8Z3_001947"
     CDS             complement(3229..3630)
                     /locus_tag="ACG8Z3_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0824254.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="ACG8Z3_001948"
     CDS             complement(3817..5625)
                     /gene="uidA"
                     /locus_tag="ACG8Z3_001948"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0824255.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5648..7414)
                     /locus_tag="ACG8Z3_001949"
     CDS             complement(5648..7414)
                     /locus_tag="ACG8Z3_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0824256.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7427..8221)
                     /locus_tag="ACG8Z3_001950"
     CDS             complement(7427..8221)
                     /locus_tag="ACG8Z3_001950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0824257.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8231..9607)
                     /locus_tag="ACG8Z3_001951"
     CDS             complement(8231..9607)
                     /locus_tag="ACG8Z3_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0824258.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9727..10374)
                     /locus_tag="ACG8Z3_001952"
     CDS             complement(9727..10374)
                     /locus_tag="ACG8Z3_001952"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0824259.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="ACG8Z3_001953"
     CDS             complement(10458..11885)
                     /gene="uxaC"
                     /locus_tag="ACG8Z3_001953"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0824260.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="ACG8Z3_001954"
     CDS             complement(11959..13038)
                     /gene="uxuA"
                     /locus_tag="ACG8Z3_001954"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0824261.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13041..14672)
                     /locus_tag="ACG8Z3_001955"
     CDS             complement(13041..14672)
                     /locus_tag="ACG8Z3_001955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0824262.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14976..15647
                     /locus_tag="ACG8Z3_001956"
     CDS             14976..15647
                     /locus_tag="ACG8Z3_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0824263.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(15807..15998)
                     /locus_tag="ACG8Z3_001957"
                     /pseudo
     CDS             complement(15807..15998)
                     /locus_tag="ACG8Z3_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(16366..16707)
                     /locus_tag="ACG8Z3_001958"
     CDS             complement(16366..16707)
                     /locus_tag="ACG8Z3_001958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824264.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
BASE COUNT         5512 a         3257 c         2249 g         5872 t
ORIGIN      
        1 gatatcaatg atgatggctc aagaaaaaaa gtggagaaag ttggcggaga cacccgacca
       61 gaggccgaag ctgctttacg aaaagtttta tcagatattg acgaaacagg acaatacttt
      121 ttaggtacgg atactcgagt aaaacaatac cttgattttt ggatggagga atacgttaaa
      181 ctaaatctaa aatacaatac ctatgaaaac taccgattta ccatcaaaaa tcatataaac
      241 ggttatttag gaaagaaaaa acttacggat ctctcccctg ctcttttaca aaatttcatc
      301 aatgctgaat ttaaaaaggg ttactcgaag aaaacaatga ctattactca ctctgtcctt
      361 aagaatgcgc tgaatatggc ggtttatcct tggggattaa tcaagcaaaa tcctatgctg
      421 tatgtaaaaa taccaaaata tgaagcacga ccaacgacta aaaaagatct aaaaatcatt
      481 tctcttgagg actttgatca tatgctagaa attactcctg aaggacatcc tttctatatt
      541 cctttgaata ttggatttta tacgggaatg cgcgttggcg aagtttgtgg tctgacgtgg
      601 gatgatgtcg atttttcaaa tggaacaatt actgtagaga aacaaatggt aaagaatgac
      661 ggcgcatggg tatatggtac gccaaagaca agcagttcca atcgaacgat ttttattgga
      721 caaaccttgc tagcaattct gaaaaaacat aagaaacaac aattagaaaa tcgaatgaag
      781 tatggaaagc tctacattga ttcaaatgca gtatgtacga aggaagacgg tgagctagtt
      841 acgccaagtg tagtgaaatg gaatactagg aggatatcga atgcactctc cctctctttt
      901 aacttccatt ctctcagaca tactcatgct acacttcttc tcgaaaatgg cgcaaaaatg
      961 aaagaaatct ctgaacgatt gggccacagc agaatttcaa ttacgatgga tacttactcg
     1021 catgtaacag ataagatgag aaatgaaacg gtcgatatca tggaaaatct tagaaagaat
     1081 tcttgatttt tgccaccgaa aaaaaccacc ggtggcaaac cggtggcaaa tccatgtaaa
     1141 catggtttat tttttacact gttttcaaac aaacccttgc tatttcttcg gttcgtcctc
     1201 gtccattttc gataccgaca gctagcttat aacccaaggt atatcaacat aaaaaaataa
     1261 aaataggcta tgacagcagc gtgacagcaa aaaagatgga aagttaaata tcaaacctcg
     1321 taatttcatt tgcgagtgct tgatttgttt ctggatataa gtgtccatat ttgtccaaag
     1381 tagttttaat agaggcatga cctagacgct tagatattgc atagatattt ttattatgtt
     1441 caattaaaaa ggcaacatgc gagtgtctta atccatgcat atgaattggt tttactcctg
     1501 caatttcagc tagttttttt attctgctta aaatagttct tggagatggc ggtaatccat
     1561 caaaagaaaa aaccaattct atatttttcc caattgattg ttgagttttt ttccatgttt
     1621 ttaaatcttc aacggtttgt ctgtctaacg aaattatccg aatacttgat gtattttttg
     1681 tttcagcaaa ttcataatct tgtcttgtac ggatgtaaat tgacttattt acgctacatc
     1741 tgccatttat taagtcaaag tctttccatt gtaacgctaa gagctcctca ctccttacac
     1801 ctgtaacaaa aataaaacgc atcaaacgtt tataaaattc ttcataaaaa tccccgtgat
     1861 aaacactatt atatactttc ttaaattcat caactgtcca aaattctact tgaggacgct
     1921 gagtttttat tttacctata gtatctacag gattttcttc aataatttca agtacaattg
     1981 cccttttaaa aactactctc aacttattga atatctgttt aatataattt ggtgataatg
     2041 gttgctcaat gccctttttt acaacatgga ttttcgataa aaactgttga aattcctgta
     2101 tatgaattgg tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta
     2161 aaatatgatc tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa
     2221 aatactctcg ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag
     2281 aaaaactttg ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc
     2341 ttctggtcac ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc
     2401 aagtgcctct ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc
     2461 caagaaatat ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta
     2521 cgataaaaaa agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta
     2581 gcatagtact gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata
     2641 gcaccttcct tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg
     2701 aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta
     2761 aatcatcgct ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac
     2821 agcaagaaat catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat
     2881 ctatttaatt ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac
     2941 tattagaaga ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt
     3001 taaattttaa gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta
     3061 cataggtaac gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat
     3121 aagaatgata atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata
     3181 agataatgga tagtggaact aataggtact gccgcttttt agatttatct accataggtc
     3241 ataccccatt ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct
     3301 catattgaga attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat
     3361 aatctttctt cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat
     3421 taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata
     3481 cctgccttca tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt
     3541 tcgaatagac ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg
     3601 tatagatatg tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac
     3661 taatacaaca atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata
     3721 tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg attaggctag
     3781 gctaatcgtt ttttgaacat tcttaaaaaa tccaaattat tttttataac tgaaattggg
     3841 tattgatgac catcgtttct gtagatagta agcaatcatt ttaggttttc tatctctagt
     3901 aaagattcct ttcttatttc cttgcactct taaaatccct tgacttgttg caaaatctgc
     3961 aaaattccac acttgttcac caacaaaatt ttcacatgta tctattactt cgtgatttgc
     4021 ttcgtaataa gccacttgat actcttctgt aaacattgtt ggtactgtat catgaaatcc
     4081 agctactgta tctgctccat attctgtaaa cattattggc gtatcaggac atctttccat
     4141 ccatccatct agctcttttt tcaacagata ttttgcatgt gccagatccc ctcccgaaac
     4201 ataccaacca taataacgat ttaaacataa aacatcgaca atatctccta ccttacaaac
     4261 atctggcgtt gattttagat aagtaacgat tgttacaggt cttttctgcg gatcaagttc
     4321 tttagttaat tcaactaaag gttcaaaata ttctttagca ccttcggaat ccgaatctgg
     4381 ttcatttgca atagaccaca ttacaacact tgggtggttc ttgtcgcgtt ctatcaattc
     4441 cttgatcacc gttttatgag aattttctgt tcgtatctct ttccacgtat tatgtttttc
     4501 cacattatca tctaataaag tggccatgaa atttaaatgt aaccctactg caggtacttc
     4561 atcaataact acaatacctt ctcgatctgc taatcgcata atttcttctg agtaaggata
     4621 atgtgatgtt ctaaaagaat tagcacctat ccatttcatt aaattaaaat ctttaatatt
     4681 tacagcctca ttcaaaccac gtccattaac atacgaatcc tcatgcttac caaatccttt
     4741 aaaataaaaa ggtttattat taatcaaaaa tttgccattg gtaacctgaa cactgcgaat
     4801 accaaattct tctgtatagg tatcgatcac tttattatca tcaattaaac taattttcat
     4861 actatataaa taagcgtcta gaggttccca aaaaattgga ttcttaagct gaatcattcc
     4921 tttctttcct tcagcttcag ctacaagttg agcctcttcg tcatatatcg ctattaagat
     4981 ttttccggtc cattctgtat cgatattgaa ttctacactt gcatccaaat cagttattct
     5041 tgtattaata gtaatatcac ttatatagtc tttcggagtt gtatataact taacaggtct
     5101 atgaatacct gcataattga agaaatcaaa attaactaag ttttcctttt ttatatcccc
     5161 attttcctgt actgtttcct tcataatccc cacaggtaaa gtactatcgt caataatatt
     5221 gtttacagca actgtaattc gattattccc gtcttttaca aaccgattaa tttcagactc
     5281 aaatggtgta aacccacctt tgtgttgagt aataaacttt ccattcaaaa aaacttttgc
     5341 ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatc ttttaggaag
     5401 agtcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa
     5461 taaatcatta taagaagagg gaactgaaat cggataactc tctttttcat ttattggttc
     5521 taaagcaagt tcttcctgta agtcatcatt tgtatctaat ttaaatctcc agagaccgct
     5581 taagtcaatt acttgcctcg tctctgtcat aatgggatac aacatataaa ccactcctat
     5641 ctaaatatta aataaatgta tcctttaaac cacaataagc atctattgga cttactccct
     5701 taaattcaga aagacccatt agtttgtcca ccaatatttc taaagtttct tgttttccat
     5761 cataagtatt aatataagtc tttacttgag gtacatcagc caaatgaaac ggacactgta
     5821 cagatacaaa aattgttgga atttcatgaa tgtaaaacgg tatatccgga gttcctttac
     5881 ttgctggcca gacaattcgt tgaactgtgc ctggattaac attagctata ttaatgatca
     5941 agtcatattt tttagttaaa tttttaattg gtctcttagc agcatatatt ccagctatat
     6001 ttttaatttt ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa atgtattttt
     6061 caacttcaaa tccttttgct gacaaaatct gtttcaataa atcaatggct tgaggtctat
     6121 ttccagcaac tacttcacca aacccacctt ttaccccatg tacatctact agtaaaatcc
     6181 ttttatattt ttctggtaca ataggccaaa tatcttgttt atctttaact aatgtaattg
     6241 cttggtcagc cacctcttta aaaattgatt tattttcaat taaaccaatt ttttccagag
     6301 cttcttgctt aggtaataaa atttgttctt ttgctttttt gtgtaatccc aatttagctt
     6361 ttaaccctaa aattcttttt aaggcatcta ataaccgatc atcagtgata attcctttac
     6421 gatagccttc catcatataa ttaaagtctt catctggatc attaaaaaat agaaatagat
     6481 cacatccagc tgcaatagaa gtgggcaata attctgatcg tttcattgcc ccagtcattc
     6541 ctaacatatg actagcatca gtaactacta atccattaaa tccgagtttt cctctaagta
     6601 agtcagtgat taattctttt gataaagttg caggaagtaa atcttcatct ttcagatcag
     6661 gattaaaatg ttttgaataa gctggcatat ggatatgacc tgccataatt gaaggtaatc
     6721 cagcttctat taagcctgag tatacttttc caaaagaatt atcccattct tccgtaccta
     6781 aactattaat actaaaagat agatgctgat ctcgttcgtc aataccgtca ccagggaaat
     6841 gtttagctgc aggcaaaatt ccactctcct gaataccttt catataagct aaactaagtt
     6901 ctaaaacttt atcagggttt tgtccaaaag atcgactaga aatgatagga tttctccaat
     6961 ttctattaat atctacaata ggagcaaaac tccagttaca tccaatagcc gatgcttcaa
     7021 tcccagaaac tctccctaat tcataagcat atttcgcatc atttgttgca ccaattttta
     7081 cttctacacc aacttcagta ccatctgtac aagctccgtt accacctgct tccgtattag
     7141 cagcaattaa tagaggaatt ttactttctg tttgtaagat atagttttga tcatatacct
     7201 catcagcagt tcctggatta tatcgtacag ctccaatatg gtactgattg accatattac
     7261 tcaagtaatt tttatcacga ctagatccca tattaacaaa caattgacct attttttctt
     7321 ccaaagtcat agaatcaatc gtctcttcaa tccaagaaat agcatcttca tctaaattat
     7381 atggtttttt tcgtaaatct acttttatag tcattttctt tctcctctac ttattagtaa
     7441 gatttttcca aaaagtatct atatattttt gttcaatatc tacataatct cccgataaac
     7501 atttaaataa aacatcagtt cttaagtttt cccatgattc tttttcttta ttgaccaaaa
     7561 taggataaaa gaaaacttta tttttatttg cagcttctag atcaccagga gaatcaccaa
     7621 tcattaatat cttatccaaa tcacaaccat tttcaattac acttcgaata atatcttctt
     7681 tttttccttt atcttgacaa cacagttcat caactaatga taataattca tgtctttccc
     7741 attcttcttg aacagcttcc ttattagctg agcttacaat aattatcttc ccatattttt
     7801 tcagagcttt tatagtatct ctagcactta aaaagggttt atctaatcct tttgcttgac
     7861 taatctgttg attgacttct tccgaccatt ttaaagcttt taataaatcc tctgactgag
     7921 aatattctat ttccctcttc aaagatggat tagacaattc ttttgttaga gatacccagt
     7981 gctttaatcg attaatcgat gttacatcat ttttttctaa tgttttaact aaacctaaaa
     8041 atctgtttat tccacgagtc tctgaatata aattaatatt attccaatcc tgtaggaatt
     8101 tttctctatg atgaacatca aaaatctctg cagcaattgg tccgaaaaag agattgtgtt
     8161 tataagtcat cgtatccata acacagccat ctgaatcaat acaaaatatc caattattca
     8221 tatttctctc ctatttatgt ttttgtttta aaagctcgca attttctaag actttatctt
     8281 tagataatgg ataaaggaaa aataaaatta atcctacaat cagaaaacaa attgatggaa
     8341 ttaatgttga agcgttatat aacttttcta aaacatctcc cgtttgtaca tttgctaaag
     8401 agtcatagcc tataatagaa agagtccaac ctccgacgcc tccagctaga gcttgaccta
     8461 tttttctagc aaaagaatat accgcatata cggtcccatc ttctctttga tcagacttta
     8521 cttcttgatc atcaataaca tcagtaatat ttgcccaaat aacagtatta aacacaccca
     8581 aacccatata gccaaccgaa ctaagaatta agaatacata catattacta gcttttatca
     8641 aaaataagat caaatatact attgctgaaa agaacattcc tactgatgcc agttcttttt
     8701 ttccatattt tttacttaaa aacaaagtta aaggcgaaac aactattaaa accaaaatag
     8761 gattgataaa attcattaaa actattccct gtgcatcatt atagtaatca ggaaatacat
     8821 aattattcat actcataatt aataattgag caaggagtaa gaaaatagcc gcaccaatta
     8881 tagaaagtaa tgccctattt ttaactaacc cttttaaaga ttttaacaat gaaactttgt
     8941 attgagattg gtcaattctt actcgttcag tagttagttt ataacatatt aaataacaaa
     9001 taagtgcaca tatcgaaaat attcccgcaa caattgtaaa agttgatcca ccacgaacta
     9061 tttgagcgcc tgaatcatct cttgtataaa taaataatgg tgttaataca ccaatgacca
     9121 aacttgctaa agtagctcca acatttcgga atgtcgatag cattgttctt tcttctgtaa
     9181 tagaagtaat agctgatgcc attgatccat aaggaatatt tatagatgtg taaaaaatac
     9241 tcccccataa tatatacgta acatacatgt atactacctt aacgctcata gaagctgaaa
     9301 tcatagaagt ttgatacatt aaaaaacttg ctaaagcgac tggaccactc attcttaaaa
     9361 tccacggttt aaacttaccc tgcttagttg ctttcgatct atcaacaata cgccccatag
     9421 ttacatcagt aactgcatcg ataaatcttg ctactaaaaa taatgttcca acggcagctc
     9481 cgcttatccc caatactttt gtataaaaga tcattaaaaa agaactagca aaaatgaaag
     9541 taaaatcgtt tccaaaatct ccaaacatat atcctatttt gtctcttata ccaaattttc
     9601 tttccataca attctctccc ttctgttgtt aagcgttttc attctgatga aagtacgtac
     9661 tatactctga agcgtaaaga gtcagtattt attttttaca tttcagagta ttatttgttt
     9721 tttctcctaa taattcttta tctcataaaa cttatctata tatttactag ctactcgact
     9781 tacttcttca aaatcacctt tttcaacaga agccaataaa ttcccaccta caccaactgc
     9841 aacaactcct acttcaaacc aatcttctaa gttctctata cttactccgc cagtaggcat
     9901 tatatttacg tgtggtaatg gagcttgata ggcttttaca atggatggac caaaagcaga
     9961 acctgggaat agtttaatga tatctacgcc agcagtaagt gcctccttta tctcagtaat
    10021 tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctcag ctgtttgtcg
    10081 atcaaaagat ggactaacga tatattctgc cccagccata attgcgatac gagcagtaaa
    10141 cgcatctaaa accgtaccgg ctccgattat tacatctttt tgatcagcat attttttagc
    10201 aatttctttg attatttctt cagcattagg aactgtaaaa gttagttcaa tccctttaat
    10261 tccacccgca ataattgaat ttattgcttt aatggcctca tccttatttt tcccacgtac
    10321 aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt tcatatctat
    10381 ctccttcctt tatggataca tactttgtct agctgtacta acttacaaag aataaattta
    10441 agcttttatt tcaagtatta ttttctactt ccgatttcaa aaaaattaaa ataattatac
    10501 gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc atctggaatt
    10561 cggcctgctt taacccactc gcttagtaat tgacataata ctcgacgaaa atactcatgg
    10621 cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa
    10681 ctttgttgtg ccatcgtagt caactgttga cgcattcctt cagctgtatc attgaaccac
    10741 caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg cattgctgtt
    10801 gctagtggaa gccaatcagt tggatttaat gaatataaaa ttgtttttgg taagcaccca
    10861 ctcttttcag cggtatcgaa caatgctaca acattttgtg tcaaactagc ttgatccccc
    10921 atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga attatttctt
    10981 aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga catcaaatgt
    11041 aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct
    11101 aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata aaaataagta
    11161 ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aatgatccac tcgttggctc
    11221 aatgcttgct tcaacccatt aaagtctttg attggtatag ccgctgtttg actaagtttt
    11281 aaaatatagt tcgaaaagcc tgatgatttg atactccaga ccttatctgg acggaaagta
    11341 ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatacca caagtcatct
    11401 gtaggatcat ccgttgtaca gatggctttc acatccatca tctccaacaa ttctttcggg
    11461 cgataggaat ccgtttgaat tttttcgtta gcacgtcgcc aaatttctgg tgcattcttc
    11521 tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa
    11581 ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt ttcataatca
    11641 tcacctggac cactgatcaa ctcttcttct ataccattag ctcgcatcaa tcgccattta
    11701 taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt gtattgctta
    11761 ttttctagca aatatgctgg gtctaaatga caatgatagt cgataattgg ttggtgtttt
    11821 gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt cttatctaaa
    11881 aacatattta aacactcctt tccttccgtt agtttttaga agagaaaatt tttcctctaa
    11941 ataaactccc ttatataatt atttactcgc ccccttttca agcgcttccc aaataccatt
    12001 taaataaact gcacctagag ctcggtcata taatccataa ccaggtctac cggtttctcc
    12061 ccaaatcatc cgaccatgat ctggacgaat atagccatta aatccattat cgtacattgt
    12121 ttttaatatc ttatacatat ctagagaacc atattctgat ttatgcggcg cttcgtaaaa
    12181 atcgagtcca ttgtcactta aatatttgat attacgaaca tgtgcaaaag gaatccgatc
    12241 ccgttgacaa aattcagata gaatttcata gacatcatta tctggtcgtt ctgcaatact
    12301 tccagtacat aatgtaatac cattagaagg agaatccact acattacaca accaatctaa
    12361 atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg gatcatctgg
    12421 gtgaactgcc attttaatcc caacttcttc acatgtcggg ataattgctt tgagaaaata
    12481 tgcaaagttc tctcgtaatt tctcttcatc aacttcttta taggcttcta ataattgctt
    12541 aatttgtgat aaccgatgag gttcccaacc cggcaattca aaattattag tagaattttc
    12601 tacttctcta ataatttcct ccggatcagt agggatattt tttgaaatat aggctaacgt
    12661 agaagaccca tcatctaaac gataatccaa atccgatttt acccaatcaa atactggcat
    12721 aaaattataa cagataacct caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata
    12781 attctcaatg tattgatctc gtgttggtaa accaattttg atatcatcat gaacattaac
    12841 actctcaata actttcaagg taagtccatt ggcttcgact tcatctttta aagatttaat
    12901 tcgttctctc ggccaaacct ctccggctgg tatatccatt aatgttccaa caattccttt
    12961 cattgctggg atctgacgaa tttcttgtag tgagattttt tcttcatttg ccccatacca
    13021 tctaaaagtc atttccatag ttactttcct ccaacttctg ttatatattt attgattgtt
    13081 ttacgaactg cttgaggacc tactagcatt tcgcaaaaga ttttttcagt tttctttcct
    13141 accccaactt gatataaatc taaaccaaag atttctttat tttctaaaat ttcctgtacc
    13201 gcacaatgaa tctttgaagg atcttgttca cctaatttca atactgataa tttatcctgc
    13261 aactctacca ataacggatc aggacttggc acaaaatttt ccccttgatc atcgactgcc
    13321 agtaaataac gtaaccaagc agcaatagcg ataggaataa atgttagatt ttctgcattt
    13381 ccgtcttttt cgacatactt tttaattgtt tcaccatagc gaatcgctac tttttgagaa
    13441 gtatctgttg caattcgttg tggtgtatct ggaatatagg gattaggcaa acgtttatta
    13501 attacttcat caataaattg ttttggatca ataatgcctg gattttcaac tactggtaaa
    13561 ccttctaaat aacctattcc tttgattaaa ccaaccaaat catcatcctg catctctgct
    13621 gaaattgatt gatatcctag cagacaacct gttactgcca aagcagtatg aagtggattc
    13681 aagcttgtcg taaccttcat gatgtctgct ttatcaaccg tctctctatc tgtcataatc
    13741 acaccagcct tttccaattt aggccggcca tttggaaaac tatcttcaat aactaaataa
    13801 tgaactacct cagtattagt gaaagcagca atattggtat gtttttttgt gtgaatgata
    13861 tctaaatcct cgagccccat catcaataga tcttccttta cattctctga aggatttggc
    13921 gtaattcgat caatcatact ccaaggaaaa gtcacttttt ctttatcctt taaataagca
    13981 ataaattttt cagaaacata tccttctttt tgccaagctt ctgcaatcgt aaaaatacta
    14041 tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc aatcggcaac
    14101 tctccagcat taaatctgct atataacaag gatgttacaa tactcatcgt atgttgagga
    14161 ttgctaggtc cagatttgat atcagctttg actaccgaaa agaattgccc tttagaatct
    14221 tttaaactgt aacctttttc agtaatagta aacgttgtaa actgtaattt aggatcttca
    14281 aaatatttaa ccactctttg atagtccgct atattcttcg gattgcaata taaactatca
    14341 gccgttactg ccaaaagtct ttttttgagt tcaccatctc cactcataac aatttccaaa
    14401 atgtcattgt tatagtcatg ataagctttt tccactactt cttcatcaaa tgtttcacac
    14461 acaactactc ctgtttctaa ttcattttga tctgctaagc attgagcaat ttctgcatga
    14521 aatcctcgat aaagatttcc tccaccaaaa tgaagccata ctggatgttg ctggccttct
    14581 tgcttaactt ctttgacatc aaaacacggc agtagaatct gcatttcatt gaattcttcg
    14641 atagaatttt gccaattttt aatcgaaacc ataattaacc tcctcaaact gatatgttag
    14701 ttttgataca ttttatattt taaaacccat tgtaaccgtt gtcaatataa aaagggaata
    14761 aacttgcttt tttctaccat atcagaatga ttgtattttt tattcagaaa aataaaatac
    14821 tccttctttt tggactgatt ttatcgaaac ttattttttc tttttttcat gtattctttt
    14881 atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa cagaattaaa
    14941 atatcagtta aaataaaagt taaggagcat tgtgtatgac aattaaaact agtctccaaa
    15001 atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa cctggaaaaa
    15061 aagtatcaga aaaaaaatta gaagaaacac tttctattgg tcgtacacca atacgtgaag
    15121 cactgattca actccgacaa caaggtcttg ttgatacaat tcctcaaagc ggaaattata
    15181 tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa catttggaac
    15241 gacaaattat gttagagtgt tgtgccaaaa tgaataacca aaaaaaacgg attttagaaa
    15301 ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt ttttttcata
    15361 atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa gtatggcaat
    15421 ggttagaaaa taacaataca cacttagaac gatttcgatg gctacgagta aaaacggaag
    15481 gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca ttaacaaaac
    15541 aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg cttaatgagc
    15601 aaaatattgt tttagctact catccaaaat atttcaaaaa tatataactt ttaactcttt
    15661 ttaaattaaa tggttgttct gatcagaaaa atatcgcttg ttttcttatt atgtaatata
    15721 tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg ataactaatt
    15781 atgactgctg ataacttttg atattaaact agtatctcca atacaaataa aaaatagata
    15841 tcttgttcat caaaattagt tcgtggatac ttaccttcat ttacatcatt cagcggtttc
    15901 ccattttctt ttattctatt atttcatttt ctaatagact tggcttcatt tcgcaatact
    15961 ttttttcaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt ttgtgacatg
    16021 ataagtatgc tcctttttct cttttccact aatacaacga tcaaagggtg cttttttcaa
    16081 aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg tttcaactac
    16141 atcttatatt tcacaataga ataaatagcc ttttgcatag ctgcttaact tataagaaat
    16201 tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat tcttcttaca
    16261 gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta ggattgatgc
    16321 cgattactta tattctacgt atagttttag tattcttatt ttttatcacc agtaaccaat
    16381 tgagaacagc cctaaagcag aatagtcttt tatctcaaaa cgcttaattt caggcttagg
    16441 aatatgaata atcttttgag tatattcttt ctcttttaat cttactgaag aaataccact
    16501 gcctatcttt tttacaaata tttcatttgg tgtacagact aataaaacaa tatttgtaat
    16561 caatccttgc actaaagaat taaaaggaag actatttaaa acagggtgtg cataactata
    16621 acatgtgaag tagttatttt gaactttaac ttttaaaaac tttaaaaata atcttatttg
    16681 ttcttctaaa caatcatcta tactcatata atcactccac taattaatct ccccaaaaag
    16741 acacagcatt aagagatata ccaacacaac agtgcaaata actctacatc ttccttcgca
    16801 accttatttc aataaaccat aaaaattatt ttttaataga aactgaaaat taaaactaga
    16861 aaaagtagtt ttaccgaatg taagagcatc
//
LOCUS       DAZKSU010000058        16537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000058 DAZKSU010000000
VERSION     DAZKSU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.058"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8Z3_001959"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACG8Z3_001959"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0824265.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACG8Z3_001960"
     CDS             complement(1174..1869)
                     /locus_tag="ACG8Z3_001960"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0824266.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACG8Z3_001961"
     CDS             complement(1882..2598)
                     /locus_tag="ACG8Z3_001961"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0824267.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="ACG8Z3_001962"
     CDS             2928..3533
                     /locus_tag="ACG8Z3_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0824268.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="ACG8Z3_001963"
     CDS             3858..4181
                     /locus_tag="ACG8Z3_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0824269.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="ACG8Z3_001964"
     CDS             4363..5733
                     /locus_tag="ACG8Z3_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0824270.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5863..7164)
                     /locus_tag="ACG8Z3_001965"
     CDS             complement(5863..7164)
                     /locus_tag="ACG8Z3_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0824271.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7394..9385)
                     /locus_tag="ACG8Z3_001966"
     CDS             complement(7394..9385)
                     /locus_tag="ACG8Z3_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0824272.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9529..9795)
                     /locus_tag="ACG8Z3_001967"
     CDS             complement(9529..9795)
                     /locus_tag="ACG8Z3_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0824273.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="ACG8Z3_001968"
     CDS             complement(10305..12431)
                     /gene="pnp"
                     /locus_tag="ACG8Z3_001968"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0824274.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="ACG8Z3_001969"
     CDS             complement(12619..12888)
                     /gene="rpsO"
                     /locus_tag="ACG8Z3_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0824275.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13139..13702
                     /gene="def"
                     /locus_tag="ACG8Z3_001970"
     CDS             13139..13702
                     /gene="def"
                     /locus_tag="ACG8Z3_001970"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0824276.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13955..14476)
                     /locus_tag="ACG8Z3_001971"
     CDS             complement(13955..14476)
                     /locus_tag="ACG8Z3_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0824277.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14479..15321)
                     /locus_tag="ACG8Z3_001972"
     CDS             complement(14479..15321)
                     /locus_tag="ACG8Z3_001972"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0824278.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15314..16039)
                     /locus_tag="ACG8Z3_001973"
     CDS             complement(15314..16039)
                     /locus_tag="ACG8Z3_001973"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0824279.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16215..16511
                     /locus_tag="ACG8Z3_001974"
     CDS             16215..16511
                     /locus_tag="ACG8Z3_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824280.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
BASE COUNT         4652 a         3458 c         3062 g         5365 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttttc ttttattcta tttttctttc
     5881 cgtggactct actttgaaat aatcgatatg tgcatgctgg tattctaata ccgtgaaacg
     5941 gaatgatgca atctcaatga ttgttcctgc ttttatttct ttatatcggc tagaagcaaa
     6001 gcccgcaagt gtagtaaaat tcgtttcttc aaactctgga atctctacat gaaaataacg
     6061 ctggaaatca tctaatgtta gtttgccgga gatgatataa gagccatctg actgttccgc
     6121 tatcaatgct tcgtccaaat ctgtctcatc tcggatttcc ccgacgattt cttctaaaat
     6181 atcttcaatc gtcactaagc cgcttgtccc tccgtattca tcttttaaga tagcaatatg
     6241 ctttcccttt tttcgcatga tcgtcaataa tttttttata gggatcgttt cagttgttac
     6301 gatcggctct tcaatcaact gttctactgt cagctggtca tctttataag attgcttgat
     6361 caaatctggc aaggtgacat aacctaaaac attgtctttt gattctaaga taaccggata
     6421 gcgagtatgt ccttgttcaa tcgaagcttt tatcgcttct ttgacggttt ctgaagcttc
     6481 gaatacttgc atggacgtac ggtctacttg tatatctttt gccaaggtat catcaaattc
     6541 aaacacgttt tccaaataat gatattcttc tttggtcaac tctccttttt cataagaatt
     6601 caatgcgact aatgtcagct cttcttgaga caatgtctct tctccttcag atacgagacg
     6661 cacacctaat agcttcccta gccctgcagc tgaatgattc aatacccaaa taaaaggaaa
     6721 cattacttta taaaaataat gaagtggttt aactacgaac aagacgacct tctccgtatt
     6781 gacgatacta tagctcttag gaagcagttc gccaataacc acatcgatat acgtaactaa
     6841 aataaatccc agcgccacag agattgtact gctaatagcc gaactaaaag gtagtttacc
     6901 gatcactggt tctagtaaag ccgcgacaga tccttcaccg acccacccga tgatcaaacc
     6961 ggttagcgta attcctaatt gactagctga taaatagtta tctaaatggt gtgtaacatg
     7021 aagagctagc ttggcgttct ttacattttc cttttctagt tgttccaatc ttgatcgtct
     7081 gattttcact aaagcaaatt cacttgctac aaaaaatgaa gtaattgcaa tcatcaaaaa
     7141 taaaataatg aaattgataa ccatattctc ccgcctttct ctttttctat tgtacccttt
     7201 ttctacaaaa agattgaaaa taatgcgtat tcatatttag gaataaaaaa ggagatgcgg
     7261 catttttcag ccacatctcc ttataacttc aaaatcattg cgttacttgt tgctaaacag
     7321 ctttttcatg acctctgata aacggtgttc aagtagctga cattcgacat tagttcaggt
     7381 caaagcgcta ctttcacaac cgctctttca accatgtttt ccgagtattt ccaatgataa
     7441 gcggaacagt ttcatgtttt acttcgctgt actctagccc aaaccatgtt tctggcgagg
     7501 tagcgatttt tttgattgcc agttttgcct gcatgatttg acgttgccat tttcctagag
     7561 ttgtcacatt ggaaactact tcatcaatca ggacaaattg gaaatcgcca acttctcgcc
     7621 ctggtgtcag agagtatctt tgtggctgtt ttggcaaacg gccttgtttc atcaaatcat
     7681 ggatgacttg gcgaacatat acgttgattt cctgttggac cctgaaacct aagaacagat
     7741 tcacttggac aataaaatca gtgtccatca tatctacttg atattctttc gtatatggct
     7801 catccgtcac ttccacatga acaaaccaat agactcgtgc tcttttaggc cgtttatcta
     7861 aaatcgaata gatgaactgt cggccaactt tatcttcagc catatcagga accaagaaga
     7921 ccacatttgt ctgatagatt ggtaaagaag tatcatcacg taattctcta agctgcggga
     7981 tataatctct caaagacaca tcctcggttg acgattcgcg aataatgttc ccttgttccc
     8041 aaataaacat cacgactaag atgatcaaac caatcagaac ggctacaaaa ccgcctcgga
     8101 agaatttgac aacacttgag ataaagaaaa cgacttctat cgcaccgaag aaaaaggtaa
     8161 ctaaatatgc aagccaacga gaatgcccat tttggataag ataaaagtaa agcagtgctg
     8221 ttgtcatcaa catcgtcaca gtgatcgata gaccgtaagc agcttccata tgatgagacg
     8281 ttcggaatgt gacaaccact agagaacagg cgatccataa aatcagattg actgctggga
     8341 tatacatttg cccgatactg ttcccaggat acatgatctt taaccgaggc agcagtttca
     8401 acttgattgc ttctgaaact agcgtaaacg aacctgtaat caatgcttgc gaagcaatga
     8461 ctgcagcgac tgttgcaaaa gcaacgccga tcacgatcca actttgaggc atcatttgga
     8521 agaatggatt caggttcttg atttgctgtg cttcagggtt ttgataaaca ttcaacaacc
     8581 acgcagcctg cccaaaataa ttgataacca aacaaatttt gacgtaaggc cagctcagac
     8641 gaatatttcg tttgccgaca tgtccaagat cggaataaag cgcctctgca cctgtcgtag
     8701 ccaggaaaat atttccaaga atgaacaagc ctaatttgtt atctgcacta aaaagcagct
     8761 ggatagcata ataaggattc agtgcacgga taactgtcca gtcttgactg aagttcatta
     8821 atcccattac gcctaaaaaa gtaaaccaag cgaacatgat tggtccgaat gcttttccga
     8881 caatatctgt accaaagcgt tggaccatga ataaaacaaa aataataact aaagtgataa
     8941 tgacgatcac gttctggtta ttaccgaacc ggtcaaaaaa ctgcggaatt cctcgcaagc
     9001 cttcaatcgc agtcgttacc gtcacagcag gggtcaatac cccatctgct agtaaggcag
     9061 ctcctccgat cattgcagga atgatcaaat atttcccacc ttttcggaca agtgtgtaga
     9121 gtgagaaaat tccaccttca ccatggttat ctgcattcaa tgcaatcacg acatatttga
     9181 ttgttgtcag tagagtcaat gtccaaaaaa tcaatgagac tgatccgata acaaaatcag
     9241 gattgattcc tgacaatccg ccattatctt caacaatcga tttcataacg taaagcggac
     9301 ttgtcccgat gtctccgtag acgactccca tcgctacaag caggccagcc attgatatct
     9361 ttttggtatt gtgcgttgtt gccacgtttt tccctcattt cttgttatat ggatatcctt
     9421 taggtaagct tgaatgatag atgatattct tatccatgat acccgctttc ctaaattgct
     9481 tctaattatc caagtaacac actgaagtgt caacttattt gaataaaatt attctttctt
     9541 tggacgttct ctgatacgtc ttgccacaaa tgaataaaga gcaaggcaaa aaattgcacc
     9601 caataatgct ggtatcaaat aaaaaccgcc gagttccggt cccagtggtc ctagtaaaaa
     9661 ttcgccgagc caactaccta aaaaaccgat aatgatattt ccagatacgc ctcccgggac
     9721 ttcctttcct aaaattgcgc cagctatcaa acctaatata ccgccgataa ataacgacca
     9781 taaaaaattc agcatctcct ttccctcctt tatccatatt tctcactttt attttacgct
     9841 ttattcagaa ggactccaaa aatatgcgcc aagaaactcc agaaaaaaag aagttgtaac
     9901 aaaagtgttc agcttatttc cgtagaagct gcttctggaa caccgtgaat cagaggttgt
     9961 gaaatcagcg gtttgacctg agtattagag aaacaaacgg aaaaatagtt cctcatattt
    10021 tgccgttttt tgagctaata tcgagggaca gctgattgaa catcgtttat tggagaggtt
    10081 gtaaaaaagc tgttctgcat caagtaataa gaacagccaa gtaaaaacag ctcctaatgt
    10141 ttttaacttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa
    10201 aaaaaggact gtgacacggc ttttgtcaca gtcccctttt ttcgaataat tattgtcccc
    10261 ctgtcacctt cacgatcaca agtacattat tcctatgaat taaattattt ttcttcattt
    10321 ttcgttgaac catcttcgtt caacattgct tttcttgaca gatttacgcg tccttgttta
    10381 tcgatttcag ttactttcac aaggatctca tcacctagtt taacaacatc ttcgacttta
    10441 ttgacacgat cgtttgctaa ttgggaaata tggacaagtc catcttttcc tttgatcaag
    10501 ttgacgaatg caccgaattt ttcgatacgg acaactttcc ctagataaac ttcaccaact
    10561 ttgacttctt tggtcaaatc ttcgatgatc ttgatagctt ttttgatcat ttctgcatca
    10621 gctgaagcaa tacttacttt tccatcttga tcgatatcga ttttcacacc agtttcatcg
    10681 atgattccgt tgatcgtatc gccgccttta ccgataacat ctttgatttt agcaggttcg
    10741 atctggatca tttcgatttt tggtgcatat tggcttaatt cttcacgtgg agcagcaatc
    10801 gttgaagtca attcttctaa gatttccata cgtgcttgtt ttgcttgagt taatgcttca
    10861 gttaggattt gttctgtgat tccttgaatc ttgatatcca tctgtaaagc agtgatacca
    10921 tcttttgtac cggcaacttt aaagtccata tcgccaagat gatcttccag cccttggata
    10981 tcagttagga tcgtatagtt ttcaccatca gaaacaagtc ccatcgcaat accggcgact
    11041 ggcgctttga ttggtacacc tgcatccatc aatgctaatg tgccagcaca gatacttgct
    11101 tgggaagaag aaccatttga ttccaatact tctgctacta aacggatcgt ataaggaaat
    11161 tcttcttctg aagggattac ttgtgctaac gcacgttcac ctaatgcgcc atgtccgatt
    11221 tcccgacgcc ctggagagcc tgcacgtcct gtagaaccaa cagaaaattg agggaaattg
    11281 tagtggtgga tgaatcgttt gctgacttcc acccctaaac catcaatgat ttggtgctct
    11341 cctagaggtg cgagtgtaca agctgataaa gcttgtgttt gtcctcgtgt gaacaaacct
    11401 gatccatgta cacgtggcaa taagctgacc tctgatgaca atggacggat ttcatccaat
    11461 ttacgcccgt caggacggat cttatcgatg gtgatcaatt cacgtacgac atctttttca
    11521 agatcttctg tgatttgttt aacttctttc aacaagtgat cttcatcttc atgacctgca
    11581 aatttctcag cataagcttc gcggatatcg atcttcacct gctcgatatt atcttcgcga
    11641 gcaagttttt cttcagtcat tactgctgct ttcattgtag cgtaagaagc atcaaagatt
    11701 tcttttttca atacttcatc aacttgcagt aaagtcactt ccattttttc tttccctact
    11761 gcttgaacga tttcttcttg gaaagcaact aactctttga tagcgtcaaa accaaacagc
    11821 aatgcaccaa gcatgtcttc ttctgaaact tctttcgcgc cgctttcaac catattgatg
    11881 gcttgtttcg ttccggcaac agtcaattcg atatctgttt gttctgcttg ttctactgtt
    11941 ggattcaaga cgtactcgcc atttacacga ccaacttcta cgcctgcgat tgggccgtca
    12001 aatgggatat ctgagattga tagtgctaaa gaagaaccta gcattgctgc catagctggc
    12061 gagcagtctg tttctacact catcacgatg ttcgttactt ggacttcatt acggaagcct
    12121 tctgcaaaca tcgggcgaat tggacggtcg atcaaacggg cagtcaatgt tgcttccgta
    12181 cttggacggc cttctcgttt gatgaaacct ccagggattt tccctgctgc atacattttt
    12241 tcttcataat tgatcgttaa tgggaaaaag tctgtatctt ttgcttcttt agaggccaca
    12301 gctgcactta agactactgt atctccataa cggactaaaa cggctccgtt tgcttgtttt
    12361 gctagctgac cgacttcgat ttgtaaggga cggccgcccc atgtcgtttt gaatacttgt
    12421 ttttctgaca taacttctcc ttttccttct cgtgaaagca agacgctact aaacaaatga
    12481 aaactgccga aagccgtctt ttgagcagct ttcatttggt tagtaggagt ccatctagct
    12541 tgatcacgga attttactta attgatatac gagtaaaaaa agtgagattc atgtgaatcc
    12601 cactcttttt gactaaaatt aacgacgcaa gcctaaacgt ttgatcaatt cgcggtaacg
    12661 ttgaacgtca gttttacgta agtaagctaa caagttacgg cggtgaccaa cttttttcat
    12721 taggccgcgg tatgaatgat ggtctttttt gtgcacgcgt gcatgttcat tcaagtggtt
    12781 gatttcttca gttaatacag cgatttgtac ttctggagaa ccagtatccc cttcatggcg
    12841 agcgtattcg ttgatgattt cattcttgcg ttcttttgaa attgccattt ctttcacctc
    12901 tttcgattat tcccctgcga ctgagtaaaa cgttggtgat tcgcttaacc aagtggcagg
    12961 tcatgtgcct agagcacaca gctatacttt acctgaaacc gggttgaaaa tcaagtagtt
    13021 aatcattaag aaaaggtagt tttcaaacaa tcttactact acttttccat ttttgtggta
    13081 aactataatg cgaaaataga cttgaaacac agtcttttaa gaaaggggta catattccat
    13141 gattacaatg gatgatatca ttcgtgaagg aaatccgacc ttacgcgaag ttgcaaaaga
    13201 agtttctctt ccattaagcg aagaagatat ctctttagga aaagagatgc ttgagttctt
    13261 aaaaaacagc caagatccaa tcaaagctga agaactacac ttacgaggcg gtgttggctt
    13321 agctgctcct caattagata tttctaaaag aatcattgct gttcacgtac caagtcctga
    13381 cccagaggca gatggacctt ctctaagtac tgttatgtat aatcctaaaa ttttgagcca
    13441 ttctgttcaa gatgcttgtt taggggaagg tgaaggctgt ttatctgttg atcgagaagt
    13501 accaggatat gttgttcgcc atgccaaaat cactgtgtct tattatgata tgaatggtga
    13561 aaaacacaag attcgtttga agaattatga atcgatcgtc gttcaacatg aaatcgacca
    13621 tatcaacggt gtgatgttct atgaccacat caacgatcaa aatccatttg cactaaaaga
    13681 aggcgtattg gtgatcgagt agagaattgg caggttcctt cgatactatg ctacaagaaa
    13741 gctttataaa aaaggggagt gctcaaaagc tgcacttccc ttttttatgt ttctccacta
    13801 atcgaaattt agatagacat ctagaaatcg caattccaaa aaattctgtg tattggatac
    13861 gcatcttttc ttattttttc ttatttcttc catatgggtg tttatctcag agatgataga
    13921 atagtttcta ggataaaaag tagcagctac gatgttatcc ttcgtagagg attcctgctt
    13981 cagctagttt ttgtttgatg tttttcagca tatgcttttc tgggtagcgg atcgtatgca
    14041 gatggatgcc gcctgttaat tcggataaca aagtagcatt acttttttga taacgtctca
    14101 taaaggaatc gatatcttgt ggcgttttga tgtgcaaagt tcctgctaac tcaccgtaaa
    14161 ggggatgttc cacaattaca tcaatgattt caccaccatt ttcaacgatt agtctcagtt
    14221 cttcttctgt ttgatctttt ccatgctgga cagcgatttt actgatgtgc ccagtattgg
    14281 tttcttctgt ttcgaggata taacctcttg ctgttgccac gatactttta cctgcagctc
    14341 gtagtaaagc aatatcgcct acaatgatct gacggctgac tccaaaagtt ttcgcaaact
    14401 tgctagcact cactggtttt tctgtatttg ccaaggtttg catgatttgt tttcgccgtg
    14461 tctctccttc gatcacactt attcctcctt cattttgttt tggtattcgc gcgagtgttt
    14521 catcaaatca acatacgtgt ctgttattgt ttccttatat cccttatttt tcagcagact
    14581 aacaacttta tccaatactt gctgctctct ttttgcatca taaactgctt ggttttcttc
    14641 ttttttcact tgtcctattt tagttaccgt atccattctt ttttctaata aagcaaccaa
    14701 ctctcgatcg atcatatcga tcttatgacg taatgtttca agagactctt ctgttagttc
    14761 ttcctctact gtcttgatga tacgagcagg atttcctgca atcactacat tgtcgggaaa
    14821 agatttggtc acaaccgaac ctgcgccgac tactacgttg tcccctagag tgacacccgg
    14881 cgtgacgata actcctccgc ctaaccaaac attattaccg attttaatgg gttttgcgta
    14941 ctccagaccg ctgttacgtt tcactggatg tagtggatgc gtcgccgtgt aaagctggac
    15001 attcggagcg aacatgcagt tatcaccgat ttcaatcgtg ctgacatcta aaaatgtaca
    15061 attgaaattt gcataaaaat tctccccaac aaatatatta tatccgtaat caaaattgat
    15121 aactggctcc atatagattt ttttccctgt acggccaaaa gtctctttca acaattggga
    15181 tcgcagttcc gctgattctg cttggttgat gatttggctt tgacttcttg caaacatacg
    15241 gtcggcaacc agctcaggat cattggcaaa ataaagctcg cctgcaatca ttttttcttt
    15301 ttcggttttg aattcagtca taatctgctc ctaattcttg tatttttttg aaaacaaccg
    15361 ctccgtcttc ttgaataaac gtataaggct ccgctaaata aacaggcgtt tgattgatta
    15421 ctaagatttt ggctgacggc tggcgaaact gtaccagatc acaaaacgga tgaacttgaa
    15481 aactcgttcc aacaatgacg atcagctctg cttggctgac ggcataagcc gcctgctcca
    15541 tgactccatc ttgaaaccct tcaccatata acacgatgtc tgggcgtatt tgtcctccgc
    15601 atgcttgatg tttgtcgctg tgaagatatt ctttccagtc taccgtttgt tgacattttc
    15661 gacaatgaca ttgatacaga ctcccatgaa aattgacgag ttgcgggctt ttagcctttg
    15721 cgtgcaaacc gtcgatgttt tgggaaacaa cccaaacatt tttttctttc gctagatgtg
    15781 ctatttccaa atgaatgata ttgggttttg cttcaggatg ataaatcttt ttgacaaatt
    15841 gatagaattt atccggttca ttcaccattg cttgatgact cagcaagtat tccggctgct
    15901 ccatcccgtg gtagattcct gacaaagatc gatagtctgg cactccagaa ggtgtggata
    15961 cccctgcacc ggtcaaaaat gtaatttgct gataagctaa tatctctttt gctgcttcgt
    16021 ctactgttaa ttgtttcatg ttttgtcacc tcactttcca ttatagccca tactcgaaca
    16081 aatttattgt tgaaaagtca gaaaaatggg aataaaaaag tgttatgggc atttgagcaa
    16141 tttatcacaa tgttcactga caaaagaatg aaaacgtgtt acaatactgc tgtataaaag
    16201 tgaggtgaat cgatatgtta ccattcttta tttattgggg cgttattctg gcttgtatcg
    16261 cctggttagc gttaagcgtt tatttctctg ttttttatct tgtacgcaaa gaaaatggaa
    16321 atctttgggc ttttgcttta ttcaacgtga ttgccgcaat tgttttagcc attacattag
    16381 ccgtatatcg aacatggggc tggggaatca cccaatactc aagtttgatt tatctaatat
    16441 tagccattta tggcgtcgtt gttcttttgc aagcgatcct aggacgtgag ccaaagaaag
    16501 cggcagctta aatgatcgag atggaactgg acacagg
//
LOCUS       DAZKSU010000059        16247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000059 DAZKSU010000000
VERSION     DAZKSU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.059"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..1586)
                     /gene="guaA"
                     /locus_tag="ACG8Z3_001975"
     CDS             complement(21..1586)
                     /gene="guaA"
                     /locus_tag="ACG8Z3_001975"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0824281.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1645..2568)
                     /gene="coaA"
                     /locus_tag="ACG8Z3_001976"
     CDS             complement(1645..2568)
                     /gene="coaA"
                     /locus_tag="ACG8Z3_001976"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0824282.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2764..3369
                     /locus_tag="ACG8Z3_001977"
     CDS             2764..3369
                     /locus_tag="ACG8Z3_001977"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0824283.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3677..4693
                     /locus_tag="ACG8Z3_001978"
     CDS             3677..4693
                     /locus_tag="ACG8Z3_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0824284.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4834..6135
                     /locus_tag="ACG8Z3_001979"
     CDS             4834..6135
                     /locus_tag="ACG8Z3_001979"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0824285.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6181..6843
                     /gene="deoC"
                     /locus_tag="ACG8Z3_001980"
     CDS             6181..6843
                     /gene="deoC"
                     /locus_tag="ACG8Z3_001980"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0824286.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            6864..7262
                     /locus_tag="ACG8Z3_001981"
     CDS             6864..7262
                     /locus_tag="ACG8Z3_001981"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0824287.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7328..8410
                     /locus_tag="ACG8Z3_001982"
     CDS             7328..8410
                     /locus_tag="ACG8Z3_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0824288.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8622..10184
                     /locus_tag="ACG8Z3_001983"
     CDS             8622..10184
                     /locus_tag="ACG8Z3_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824289.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10177..11310
                     /locus_tag="ACG8Z3_001984"
     CDS             10177..11310
                     /locus_tag="ACG8Z3_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824290.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11310..12266
                     /locus_tag="ACG8Z3_001985"
     CDS             11310..12266
                     /locus_tag="ACG8Z3_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824291.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12365..13531
                     /gene="deoB"
                     /locus_tag="ACG8Z3_001986"
     CDS             12365..13531
                     /gene="deoB"
                     /locus_tag="ACG8Z3_001986"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0824292.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13555..14373
                     /locus_tag="ACG8Z3_001987"
     CDS             13555..14373
                     /locus_tag="ACG8Z3_001987"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0824293.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14390..15097
                     /gene="deoD"
                     /locus_tag="ACG8Z3_001988"
     CDS             14390..15097
                     /gene="deoD"
                     /locus_tag="ACG8Z3_001988"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0824294.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15395..16081)
                     /gene="gpmA"
                     /locus_tag="ACG8Z3_001989"
     CDS             complement(15395..16081)
                     /gene="gpmA"
                     /locus_tag="ACG8Z3_001989"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0824295.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT         5043 a         3004 c         3618 g         4582 t
ORIGIN      
        1 gatttctagc tctcctacaa ctattcccac tccactgttg ctggtggttt agaagtgatg
       61 tcatacacga tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc
      121 aagacttccc atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca
      181 cggataccga ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc
      241 cctggaagaa cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct
      301 tcacgcaaga tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca
      361 agaacacgaa taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc
      421 atgcctaact gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg
      481 atcaattcaa attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc
      541 tgtgctgttt ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa
      601 gagacgcctt cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca
      661 ttcccaatga ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag
      721 cgatctcttg catcgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt
      781 acttgttccg cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg
      841 tcaccgatcg ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga
      901 ccaagcaaga cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata
      961 aagttatcca ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc
     1021 aattcgattc cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta
     1081 cgtgttgtgt cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag
     1141 ccttcaggaa ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt
     1201 gtttctttaa acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg
     1261 cggtttttag caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag
     1321 caaatcccta atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc
     1381 tcatcatata cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt
     1441 acttcttctg cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata
     1501 cgtcttgtga tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg
     1561 ttcgtcatat cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca
     1621 aaatagtcaa actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg
     1681 attttcagtt ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg
     1741 taaatttttc aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg
     1801 atctccgata gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa
     1861 cgcaccaaaa cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac
     1921 agaaaaatca aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt
     1981 gatcccctca acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat
     2041 atcatacaca tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg
     2101 gttcaagaaa tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat
     2161 gattccacgc tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg
     2221 aacgtttctt cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact
     2281 ttttccaacg gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt
     2341 atgaagaaat aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat
     2401 cagatgagca agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa
     2461 acttttgata ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc
     2521 tcgccattct tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg
     2581 cgttctccca tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga
     2641 gagattgccg atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga
     2701 cttgcgcacg gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa
     2761 gacatgacaa atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg
     2821 gcttttgagt taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt
     2881 gaaacagtgg attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct
     2941 gatgaaggaa agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca
     3001 tttgcgacac agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg
     3061 caaggaaacg caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat
     3121 gaagcagttc atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga
     3181 aaaaaagttg tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca
     3241 ctgacaattg tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa
     3301 acatttggta atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg
     3361 aaagaataac tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta
     3421 cgggtacgta tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc
     3481 ttcaaaaaga aataatagga acagagtcgt tgttttttac gataaaataa ataagataca
     3541 gaacttgtat agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac
     3601 tttccttttt tctctattct tttcttgggg gttttggtac actgataggg aatttagata
     3661 gaagggagcc aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc
     3721 agtaacaact gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag
     3781 aaaaaaagtg ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa
     3841 tgtgatcaat cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt
     3901 cttctccaaa ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat
     3961 gttatgcaat tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca
     4021 ccgctcagtg gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt
     4081 agacagcggt ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg
     4141 ggataacgga cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt
     4201 tattgaaaat ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt
     4261 agaagaacgt ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg
     4321 gaaatttgtt gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt
     4381 actcaaacat aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg
     4441 ctatcaggcg atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt
     4501 cgatggatta aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag
     4561 ttttgatatt ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag
     4621 aaaagttccg aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt
     4681 gacaaagggc taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg
     4741 ctttttttat ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt
     4801 ataataccga atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa
     4861 aagagggatg gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg
     4921 aatggaaaga ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac
     4981 atgacaaatg aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc
     5041 gatttgagtt ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac
     5101 acgaccacat tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg
     5161 tctggacgcg gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga
     5221 tttcagatcg agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg
     5281 gtaattggtc aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac
     5341 gtcacagcga ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc
     5401 gcggcaggag cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag
     5461 aacatcgaag atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac
     5521 cgagaaacag tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat
     5581 agtctagaag ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta
     5641 gaaatgtgct acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc
     5701 gatgaagcaa gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc
     5761 aaagaaatga tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt
     5821 ttgactgcac gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc
     5881 gtggcaaatg aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa
     5941 gaagatattg atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca
     6001 aaaggtgagt cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa
     6061 ttattatata aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac
     6121 atcatcacag aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa
     6181 atggaactaa accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct
     6241 gtgatgcaaa ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca
     6301 gcatgggttg cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta
     6361 atcgggtttc ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc
     6421 atcaataacg gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag
     6481 gatgatcaac aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg
     6541 cttgtcaaag ttattattga aacatcgtta ttagatagag aagaaatcat ccgagcttgc
     6601 gaaatagcaa aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc
     6661 ggagcaaagg tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc
     6721 aaagcttccg gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct
     6781 acaagaatcg gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat
     6841 taattttttc aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta
     6901 ttgaagcgtt agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg
     6961 taacggaatc aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa
     7021 cgaattgtgc cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta
     7081 cgcatctagt cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac
     7141 aagtaatggc agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg
     7201 tgacgaaggc aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat
     7261 aactcatgtt cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt
     7321 tacagaaatg aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt
     7381 atttttaggt gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc
     7441 agacacaaca actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa
     7501 ccagtcagct tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa
     7561 tgatggcttc caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca
     7621 agcattgaat gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat
     7681 ccaagaacaa gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga
     7741 tggattagat aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg
     7801 tgttgcagca gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg
     7861 tgaagtaatc gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga
     7921 attaggaaaa gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa
     7981 aggacgttca attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc
     8041 aggcggtaca ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa
     8101 aaaagtttgg gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa
     8161 cggcgaaaag aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga
     8221 agatttggca caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg
     8281 attgaaagaa gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc
     8341 agttgatgaa gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga
     8401 agaaaactaa aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg
     8461 attagaacaa gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca
     8521 actccctgaa gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca
     8581 atcgttgccc tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg
     8641 tcatagaaat gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca
     8701 atttacaagt aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat
     8761 ctacattgat gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc
     8821 gtgggaaaga agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg
     8881 tccatcagca ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa
     8941 gcgagcctag tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag
     9001 tttctgaaca gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg
     9061 gaatggaaca acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt
     9121 ttgatgagcc gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga
     9181 aggaattagt caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca
     9241 aagcagtagc tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg
     9301 ttaaagacgt aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca
     9361 aaacgatgaa aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg
     9421 tgaaagaaaa tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg
     9481 aagtacttgg tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga
     9541 ctggtttgcg aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata
     9601 aaaaaccccg aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat
     9661 acgggttagt cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa
     9721 aaccttacag taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat
     9781 tgatcgaaga atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag
     9841 gcggaaatca gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga
     9901 ttgttgcaaa tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc
     9961 tgatcgaaca aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa
    10021 ttttaaatgt ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg
    10081 atccgaaaga aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag
    10141 aagcacgaaa agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc
    10201 cgtaatttat tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg
    10261 atggctgctt ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt
    10321 ggttcgcaaa gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca
    10381 ttaggttttt ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca
    10441 ctttgcgggt ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt
    10501 gttttgcttc cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt
    10561 ccaggtttac ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac
    10621 tatatcttat tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc
    10681 tcaagtttgg actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta
    10741 acacaagctt ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt
    10801 attttcgttt ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga
    10861 ttgaatcctt ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg
    10921 atggtcattt ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat
    10981 caaaacttct ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc
    11041 ttgcttggtt ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg
    11101 aagatcggtg gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta
    11161 tcgattgctt tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct
    11221 aaagtcttcc aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg
    11281 aatcaaaacc atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc
    11341 ttacttctac actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt
    11401 ctgaacgaag cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta
    11461 gttccatcgt tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg
    11521 ctattttagt gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag
    11581 tcagtttgcg tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt
    11641 taggtgtttt cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga
    11701 atctggggta tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct
    11761 ttaaaaatac attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga
    11821 tcatcttcaa gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg
    11881 ccgacacatt aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct
    11941 tgttaggcgg tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg
    12001 gtacgatcgt tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc
    12061 ctttaggggc aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg
    12121 gcgcacaatt accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt
    12181 tgctgacaat cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg
    12241 ggaagaacta tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa
    12301 gtgtttttcg tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat
    12361 aatcatgttt aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc
    12421 agatgccgat aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc
    12481 aggattgaac gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg
    12541 tgtggaatca gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg
    12601 gaaagatacg atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg
    12661 tgtgtttcct aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg
    12721 taaagtcgta tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca
    12781 ccaaatgaaa acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc
    12841 ggcacatgaa gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga
    12901 tataacaaaa gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga
    12961 accaggagac ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca
    13021 cacagtcctt gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa
    13081 cgatattttc aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg
    13141 cgttgaccag ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt
    13201 agtagatttt gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat
    13261 tgaagaattt gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct
    13321 aatgattaca gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga
    13381 atatgtaccg ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg
    13441 atattattca gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa
    13501 tggcgaaagc ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc
    13561 aagttaagcg agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa
    13621 attgattttg gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat
    13681 gctgtaatga tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat
    13741 gcaggaaagc tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga
    13801 ttccactact atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc
    13861 ggactagggg cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt
    13921 gtaccaggag atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg
    13981 atcggtccga atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg
    14041 cctgactatc aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa
    14101 ggcgtatata tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct
    14161 cggacaatag gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct
    14221 cacagcggat taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa
    14281 gcgaatctga gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa
    14341 gcattggtta aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca
    14401 tattgaagcg aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg
    14461 agctaaatat attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg
    14521 tatgttaggc tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat
    14581 gggaatgcca tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa
    14641 attgattcga gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt
    14701 actagcacaa ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca
    14761 ttttcctcca atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa
    14821 gggctatacg gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt
    14881 gacagagacg ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc
    14941 tctttattac ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga
    15001 ccatttgatc accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat
    15061 gatcgaagta ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct
    15121 gcattaagta ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga
    15181 cttattattg caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt
    15241 ttaactctga gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg
    15301 tgacttgtga ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac
    15361 aaatcgtgtc acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg
    15421 ttaagtcatc atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga
    15481 tgtcttcgtc agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg
    15541 cagctactaa aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg
    15601 gcaaagcgcg ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta
    15661 acattgcgta acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag
    15721 gaagagtatc gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg
    15781 tttctttttt gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt
    15841 tgatttgtgg tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg
    15901 cacgagttaa aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc
    15961 gtccgccttc ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag
    16021 taaattggtt caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca
    16081 tgtttagctc tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg
    16141 tacaattttt tggaagagat ttccatcgaa aaattagata agattctatg agaaaacaag
    16201 tagagaatat aaaaaaacga acagtgccca cacaactgtt cgttttt
//
LOCUS       DAZKSU010000060        16157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000060 DAZKSU010000000
VERSION     DAZKSU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.060"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(286..2013)
                     /locus_tag="ACG8Z3_001990"
     CDS             complement(286..2013)
                     /locus_tag="ACG8Z3_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0824296.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2006..3199)
                     /locus_tag="ACG8Z3_001991"
     CDS             complement(2006..3199)
                     /locus_tag="ACG8Z3_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0824297.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3386..4030)
                     /locus_tag="ACG8Z3_001992"
     CDS             complement(3386..4030)
                     /locus_tag="ACG8Z3_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0824298.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4124..4258)
                     /locus_tag="ACG8Z3_001993"
     CDS             complement(4124..4258)
                     /locus_tag="ACG8Z3_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0824299.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4260..6392)
                     /gene="feoB"
                     /locus_tag="ACG8Z3_001994"
     CDS             complement(4260..6392)
                     /gene="feoB"
                     /locus_tag="ACG8Z3_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0824300.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6389..6862)
                     /locus_tag="ACG8Z3_001995"
     CDS             complement(6389..6862)
                     /locus_tag="ACG8Z3_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0824301.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7263..7487
                     /gene="nrdH"
                     /locus_tag="ACG8Z3_001996"
     CDS             7263..7487
                     /gene="nrdH"
                     /locus_tag="ACG8Z3_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0824302.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7646..9805
                     /gene="nrdE"
                     /locus_tag="ACG8Z3_001997"
     CDS             7646..9805
                     /gene="nrdE"
                     /locus_tag="ACG8Z3_001997"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0824303.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9855..10820
                     /gene="nrdF"
                     /locus_tag="ACG8Z3_001998"
     CDS             9855..10820
                     /gene="nrdF"
                     /locus_tag="ACG8Z3_001998"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0824304.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10909..12048)
                     /locus_tag="ACG8Z3_001999"
     CDS             complement(10909..12048)
                     /locus_tag="ACG8Z3_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0824305.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12357..13070)
                     /locus_tag="ACG8Z3_002000"
     CDS             complement(12357..13070)
                     /locus_tag="ACG8Z3_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0824306.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13324..14025)
                     /gene="nagB"
                     /locus_tag="ACG8Z3_002001"
     CDS             complement(13324..14025)
                     /gene="nagB"
                     /locus_tag="ACG8Z3_002001"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0824307.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14259..15791
                     /locus_tag="ACG8Z3_002002"
     CDS             14259..15791
                     /locus_tag="ACG8Z3_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0824308.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15982..>16157)
                     /locus_tag="ACG8Z3_002003"
     CDS             complement(15982..>16157)
                     /locus_tag="ACG8Z3_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0824309.1"
                     /translation="FMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQT
                     VVFKGKRNLENKK"
BASE COUNT         5001 a         3339 c         2828 g         4989 t
ORIGIN      
        1 tttaatcact tttaagtata taaactatgt atattcttta atattttttg agaatgcact
       61 tcgtacataa taaactttaa tcacattagt ctttcatacc tacgatcgat ctctttgact
      121 cgcacccata aattcataat agtttgtcgg cgtctttgaa cgtttcgaag atgagctacg
      181 cattctcttt ttttatagac aaaaaagata gagacgaaca tggagcttcc aaatttggat
      241 atttgatcca attttggaag ctccatttat ttcgtgtctg tctttttatt ttttccagct
      301 ttcgactaat tcttggttct ctttgatcca gtcttttgct gcttgctgtg gatcttttcc
      361 attgttgatt tccaacataa ctttttccat atctttttct gaccaatgga aattgtctaa
      421 tactttgtaa gcttctggct ggtcttcttt caatcctttt cgaaccatcg tatggatctg
      481 ctcttcgctt cccattccgt tttcaggatc ttctaagtat ttcagatcat atttggcaaa
      541 catccaatga ggtgtccatc ctgtaactac aattggttcg tgttttttga tcgcttgacc
      601 tagtgcaacg gtcattgctc ctgaagaaga tgtctctact ttccagtctt tcaagttgtc
      661 atatttttgg atcgtgtttt cagcagccgt aactacacca gctcctggct cgattcctat
      721 gattttctta cctgcctgat cggttaactc atcgatcgaa tttacatcca tgtaggctgg
      781 cactgctaaa ccaacttttg ctcctgtcaa attggcacct aggtcttcga cttgatcttt
      841 atattgtgca tattgtgaac catgagtttt aggtagccaa gctgaaacca tggcatctga
      901 ctcgccattt gaaacagatt tccacatgat tgaattatct aatggcgtca tcgttacatc
      961 atagcccatt tgtttcaata cttcaccgac tacatttgtt gaagcaactt ctgtatccca
     1021 ttcaacataa gataagttga tacgttttgt ttctttggca gatgaaaaac tccctatacc
     1081 aatcgcacct aagatcacta ctactacagc accaatgatc agtccttgac gttttttact
     1141 tttctgtgtt gttgctcctg cagctttttt attgtttggc tggttcaaat gctgtgtaaa
     1201 acggtcaatg atgattgcta agataactaa tgcaaccccg ttgacaaatc cattcccgac
     1261 ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag ccgggtgcac cgatcataga
     1321 ggcaataacg accattgaaa gtgacagcat tgttgtttga ttgatccctg ccatgatcgt
     1381 acttttagct aaaggcaatt ctagtttgaa tagtttttga cgacttgtgc tcccgaatga
     1441 atcagaagct tctactaatt ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac
     1501 ggttggtggc agagcaaaga tgactgaggc aaatacccct ggaaccatcc cgataccgaa
     1561 aaatgcaact gctggaatca aataaacgaa tcctggcatt gtctgcataa agtcaaggat
     1621 cggtttgata atactttggg ctttactgct ttttgccatc aagatcccta atggtacccc
     1681 aatgatgatc gagatcacac tagataaaag aacaagtgtg acagtattca tcaaatcatt
     1741 ccatagacct tggttataaa taaaccatag accgattaat gtgaacaacg taagtcctgg
     1801 acgtttctta gaaataaaga aagctgctac cgtcaataac aagataaaca aaggagctgg
     1861 aatcactaac agtaaatttg taatccccga catcactgcc tgtccaatcg tttgtaaaaa
     1921 tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg atattttcta cccagtttgc
     1981 cactggtaat tgatagttca atagattatt catgttcact cacctcgctt gtttctgcta
     2041 atgcttcaat cacactacct cggatgacga caccttttaa ttttccatcc tctacaactg
     2101 caagtggagt cggtgagtca taaataatat tgaaaatatc acttaccacc atgtctttcc
     2161 caatcaccgt cacattttca tctaggaagt cgatcaatgg acgtttttgc ttcctagctt
     2221 ctaacgcttg atctgcagtg atgatccctt tcaggtgtct ctttctgtca acagccatca
     2281 gcatgctgac ttcttcatta cgcatccttg tcaatgcaac gtttggtcca tcagattcga
     2341 tattcgtcgt caatgcaggt accatgatgt tttgagccgt aagcactttg gaacgatcga
     2401 cttcttccac aaattcacgt acataatcat ttgctggatt ggtcaatatt tcttcacctg
     2461 ttccgatctg catgatttcg ccgtctttca tcaaggcgat tctgtcccca atccgtaaag
     2521 cttcattcaa atcatgggta atgaaaataa tcgttttttg aacattggct tgcaggtcca
     2581 gtaattcgtc ttgcatttct cgccggatca gcggatcaag agccgagaac gcttcgtcca
     2641 tcagcaagat ctcaggatca tttgctaaag ctctggctag gccaacccgt tgttgcatcc
     2701 caccagataa ctgactagga tattggtctt taaatgaaag caaactagaa ttttctaatg
     2761 ctttttctgc tttttcttgt cgttcttctt taggtacacc acggacttct aacccgtatt
     2821 ctgtattttc caagatcgtt cgatgtggaa acagaccgaa gttttggaaa accatattga
     2881 ttttatgacg acggacttct cgcaattctt ctttgcttaa cttcgataca tcttctccat
     2941 caatatagat actgcccgaa gttggctcaa tcagacgatt caatagacgg atcaatgtgg
     3001 atttcccact cccggaaagc cccatgatca cgaagatttc tccttctttt acgtcaaaat
     3061 tcacgtcata cacaccaacc gttgcaccgg ttttctttaa aatctccgtt ttcggctggt
     3121 gatcattcat catatctaaa gcggcttgtg tctttttacc aaatatcttt gttaaatgtt
     3181 ttacttctac ttttaccaaa caaattctcc ttttttatgt aaattcattt ttctttctga
     3241 aaaaagtgac cacactatgt taacatcatg tagtcacttt gtcaaaaatg aaagaaattt
     3301 gcttgtccaa actgaaaaaa acttggctaa atctttgtaa ataaaaggtt tagccaagtt
     3361 aatatttttt taatatttca cacaatcatt ctgcaagctg atgctccggg taaaaaaagt
     3421 ttgtcatacg ctgtagtgta tattcatttc caataaaatg aacgatgtcc cctgccgtaa
     3481 atttagcata ggggccaggt gaaacaagca gttcttcatt atgagaaatt gcgataactg
     3541 tggcgccagt cgcctgccag atattcaact cgctaattga acggtccaga tgcattgctt
     3601 tttctgataa agataattca aacggaagta aaggattcat cttatgtatt cgcttcgttt
     3661 gattgaccag ctgatccaat aattgagaaa aatgattcag ctcatcttgt tgcttctcca
     3721 cacttgccat gatttctgct tttgtatctt ggatggattg tacatcttga tattgcaata
     3781 ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc acttccatgc cgtacttcca
     3841 tgattccaag atctaccagc acattgatag ccttgcgggt agtctcagga gaagcattgt
     3901 acgtactggc aagtgttgag cgggcatgga tcttctcacc cactttatat cggttttcaa
     3961 cgatacgttc ggctaaatca acagcaatct gctggtagcg cgggcggacg atttttttct
     4021 ttccagacat tttctttcct ctttcttttt ttactttccc tcatcttact atgctctagc
     4081 aaaataagca aaaaagggga gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt
     4141 ccttttttac tgggcaagct gtttgacagt catcacaatt cttaccggat tttaaacgag
     4201 tatagacaat tgcgcctgca cttccaaaga ttaacacact taataagaat gtcgccatcc
     4261 tattcctctc cttctaatga caatatagta taaaactggt tcttggattt tggctgacgg
     4321 aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa gatacgtccc taacgctacc
     4381 tgtccaccct ccaataagtg tcccaactga taaatcacaa aactaactaa ataagctaaa
     4441 ccgcattgat agcctatggc gatccaagtc catttcttgg cattcatttc ccgatggata
     4501 gccccaattg ccgcaaaaca aggcgcacaa agtaaattga aggctaacaa tgaataagct
     4561 gctgcgggag tatatgtgtg ttgtagcact tgccagattt ctctgccatt ttcggatact
     4621 tccgcatgac caaacaaaat accaaatgtg ccaataatat tttctttagc gaccaatcct
     4681 gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc
     4741 aggattcgcc cgaaaaaggc taagatactt tggtcttcag aaacaggttg agcataaaaa
     4801 ttataagaag agctgaacca gatcacgata ctagaaacaa agataatcgt tcctgctttt
     4861 ttaacaaaag acttgctgcg gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa
     4921 tgatatgcgg gcaattccat gataaaagga gctgtatctc cagcaaacaa acgtgttttt
     4981 ttcaagctga ttccagagaa aataattgca gtcataccaa gaaaataggc agaaggagct
     5041 acccaagatt gatgaggaaa aaaggcccct gctactaagg caataatcgg caactttgca
     5101 gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac gatcttgctc gttttcaatc
     5161 gtgcgactgg ccatgacgcc tggcactcca catcctgtag caatcagcat aggaatgaag
     5221 gattttcctg aaagaccgaa tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc
     5281 atatatccgc agtcttccag aaagctcaag cataaaaaca atataatcaa ttgcggtaga
     5341 aatcctaaca ctgctcccac gcctgcgatg atgccgttca agatcagctc ctgcatccat
     5401 cccgctactt gccattcagc taacaatctg ccaacatgtt ctggtaacca tgtaccaaac
     5461 aatgtgtcat tgatccaatc tgtccccatt gttcctactg tttgaatagc cagataatag
     5521 atcagccaca tcacaaatgc gaagatcggc aaggctaacc aacgattcgt tgcaatctga
     5581 tcgattttgt cactcatcga taattggaaa gaatcatttg acacaacaca gagtgtacat
     5641 agttgggtaa taaattcata acgttcattg accagaatcg tctcactatc gtcgtccaat
     5701 agtttctctg ttaactggat cagttgctcg atctcttttt gctgagaaac tgctatctcg
     5761 taatcctctc ttaccttctg atctcgttca aaatatttca ttgcatacca gcgtttcttg
     5821 ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag ctgcttctaa acgtggatca
     5881 tatgtacagg gaacaggaat agaaggccgc ttttttgcag actcctgaat cttttcgatt
     5941 agtttttcga ttccttgctt tttcaatacg ctgattggta aaactggtac accaagacca
     6001 taggaaagct tttctagatt gatttttttt ccttcttttt tgatgacatc catcatattc
     6061 agaccaatga tcataggaat gtctaaatcc aataactgcg tagttaaata aagatttcgt
     6121 tctaaattgg tagcatcaat aatgttgatg atcagttcag gctgttcctg aaccaaataa
     6181 tctcttgcaa tcacttcttc tggtgtataa ggagacaaag aatagatgcc gggtaggtcc
     6241 tgaatatgaa tgttcggatt ttttttcaac gatccttctt ttttttctac tgttacgccc
     6301 ggccaattcc ctacatattg attcgaacca gtcaagagat tgaacaagct agttttcccg
     6361 ctgtttggat ttccgactaa cgcgatattc attctttttc ctccctttgt atcaagatta
     6421 gttcggcctc tgactttcgt aacgtcagtt cataccctcg cactgtcagt tcgattggat
     6481 ctcccagagg ggctagtttt cggataacta tttttacatt tcgcgtcaat cccatatcca
     6541 ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac gacgatccca gtctctcctg
     6601 cttttaattg atcaagagaa gtccagcttt cagaggaagc agtcttttta ttgacaaaga
     6661 tttgacctag tatttcccga cttactgcca ctcgatgact ttttaccagt aaaatggcat
     6721 tgttgcctga taaattgacg acgacgattt tacttccggc aacgatcccg atattttgca
     6781 aatggcgttt tgttttttct tctgtttcga ttcgttcgac ttgataaacg aaattctgct
     6841 ttgcctgcgc taaggtctgc atattgattc ctctcttgtg aaataaatca tgtattaaaa
     6901 tcattcatta tatgttctaa ttttcttacg tgttacgcaa ataaatgaga atcattctca
     6961 catagaatac tctcggtaat tgagaatgtc aattatactt gtgatattat gagcgaatct
     7021 ttagtattga tagataaaaa tgagaaaaaa atatttcttc atagttttcc acaatttttt
     7081 ctctattaaa tttgctattt tcccacacga tacttgtttt cagctctttt ttcaaaaaca
     7141 caaaacttag tattccacag tttccacaac acaatatgta gtgtttgttg tttgactttt
     7201 ttactaagag ttttatacta tctattatag ataataagaa tgtgttagag gaggcactca
     7261 ccatgaacgt aaaaatcttt tctaaaaata attgtatgca atgtaagatg gtcaaacggt
     7321 ttttaacaga aaatcaaatt gcttttgaag aagtcaatat cgatgaacag cctgaagcag
     7381 tagactggtt gaaagcacaa ggattccaaa gtgttcctgt cattacttct gatgcagaaa
     7441 cagttatcgg attccgtcct gatcagttgc gtaaacttgc cagctaacat gactaaaaga
     7501 atcaaaacgt gatacatgga tttgaagggc ttgagacagc accagttttt gtcgcaaact
     7561 tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa
     7621 tacgaaatga aggttgggcg tacgtatgag tcttaaaaat ttacaagatg tcagttattt
     7681 caagctgaat aatgaaatca atcggccagt caatggtcaa atccctttga ataaggataa
     7741 agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca aatacgatga cttttccatc
     7801 tattatggaa aaaatcaatt acttgatcga tcaagaatat atagaagcag aattcattca
     7861 attgtattca ccatcattta ttgaaaagct atatcaattt ttggacgagc aacatttttc
     7921 tttcaaatct tttatggctg cttacaaatt ttattctcaa tatgcgttga aaaccaacga
     7981 cggttccgct tatctggaaa cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc
     8041 gaacggggat gaagaattag cacttgctct tgcagacgaa atgatccatc aacgatatca
     8101 gccagctaca ccttctttct tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg
     8161 tttcttgact caagtcactg acgatatgaa ctctatcggg cgctcaatca actctgcttt
     8221 gcaattgtcc cgtattggtg gcggcgtagg gattacttta tcaaatcttc gtgaagccgg
     8281 cgctcctatc aaaggatatg agggtgctgc aagtggtgtc gttccagtca tgaaattatt
     8341 cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt caaggagctg gcgtggttta
     8401 tttgaacgtt ttccatccag acattattgc tttcctttca gcaaaaaaag aaaatgcaga
     8461 tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc gtacctgata aattttatga
     8521 attgacacgt aaaaacgaag atatgtattt atttagtcca tatagtgtcg aaaaagaata
     8581 tggtgtccca ttctcttatg taaatattac agctgagtat gatcatctag ttgcaaatcc
     8641 aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca
     8701 acaagaatct ggctatcctt acgtaatcaa tattgataca gcgaaccgcg agaatccaat
     8761 cgatggcaag atcatcatga gtaatttatg ttcagagatt ttacaagttc aaacgccttc
     8821 tgtgattaac ggcaaacagg aatatgaagt attaggaaca gatatctcat gtaacttagg
     8881 ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat
     8941 gactcgtgca ttgacattta ttacagatgc atctgatatc gatgtcgtac caccgatcca
     9001 aaacggaaat aagctgaatc atacgattgg attaggtgcc atgggcttac acacttatct
     9061 tgcaaaagaa caaatggaat atggctcaga ggattcatta gactttacaa atatttactt
     9121 catgctgttg aattattgga cacttgttga aagcaacaat atcgctcgtg aaagaaaaca
     9181 agttttccat aattttgaaa aatcagctta tgctgacggc acttatttcg aaaaatacgt
     9241 aactggagaa ttccagccaa aatcagaaaa agtcaaagca ttgtttgaag gcatttttat
     9301 cccaactgct gaagattgga atgctttgaa acaagcagta atgaaagatg gactgtatca
     9361 tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca tatatcaacg atacgagtgc
     9421 aagtattcat cctatcactc gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat
     9481 ttattaccca gcgccttatc tatccaatga gacgattccg tattacactt ctgcttatga
     9541 tatggacatg cgtaaagtaa tcgatgttta tgcaacggca caacaacacg tcgatcaagg
     9601 catgagtttg actttattca tgcgttctga aatcccagaa ggcatttatg aatggaagac
     9661 aacaacgaag caaacgacac gtgatttgaa tattttaaga cattacgctt tccacaaagg
     9721 aatcaaatcc atttattacg tacgtacctt tactgatgat gcagaagaaa tcggaagcaa
     9781 tcaatgtgaa agctgcgtga tctaatatca tagatagatt cgcttattca tattttaatt
     9841 aaaggagcat tctcatgtca gaaacatatt atgaagcaat aaactggaat gaaattgaag
     9901 atatcattga taaatccact tgggagaagc tgacagagca attttggcta gatacacgta
     9961 tcccactttc aaatgaccta gatgattggc gtacattgtc tcaattggaa aaagatacag
    10021 taggacatgt atttggcggt ttgacactgc tagatactgt ccaatctgaa agtggcatgg
    10081 atcagttgcg taaagatgtc cgcactcccc atgaagaagc cgttttgaac aatattcaat
    10141 tcatggaatc cgttcatgcc aaaagttatt cttctatttt tagtacactg aatacgaaaa
    10201 aagaaatcga agaaattttt gactggacaa atacgaatcc ttatctgcaa aaaaaagctg
    10261 agcgtatcaa tgaaatctac aaacacggta caccgctaga aaagaaaatt gccagcgtct
    10321 ttttagaaac attcttgttc tattcaggtt tttatactcc tctttattat ttaggaaaca
    10381 acaaattggc taacgtagca gagatcatca aactaatcat ccgagatgaa tctgtacatg
    10441 gaacctacat cggttacaaa ttccaattag gattcaacga gttacctgaa gcagaacaag
    10501 aaacgttaaa agactggatg tacaatcttc tttatgagct ttacgaaaac gaagaacgtt
    10561 atacagaaga attatacgat ccaatcggct ggacagaaga agtcaagaca ttcctacgct
    10621 ataatgccaa caaagcattg atgaacctag ggatggatcc actctttcca gatactgcca
    10681 atgatgtcaa cccaatcgta atgaacggga tttcaacagg cacaagcaac catgacttct
    10741 tctcacaagt cggaaacggc tatttgttag gtcatgtaga agcgatgaaa gacgacgatt
    10801 atttgattgg attggaataa ggatttattt taattcaaaa atttttcgaa aaataaaaag
    10861 agtttagcgt acagatattt taaatgtaca ctgaactctt ttttatgctt attcacttgt
    10921 ttcttccagc acttttggac tgtctaactt ttccgaaaca tgttccattc ttctcaataa
    10981 atagattttc cggatattta atattatcgt cttaatcact gcatagacag gtatgcaaat
    11041 cagcatgcct aatattcctg cgacacttcc tgctccaatc aataccaaga tgatcgtcaa
    11101 aggatggata ttcattgatt tcccaaataa caacggcttg atcaaatttc catctaattg
    11161 ttggatcact aaaatcgaca acgccatata caaagcttga aatggagaga taaacaaacc
    11221 gacaatgatt gctggtaccg ctccaataaa agggccgaca tacgggataa tattcgtgat
    11281 tccgcaaatg accgcaagca acaagctata aggctgatcg aatattctca ttgccaaaaa
    11341 gctcattaca ccaataatca aagcatctaa gatcgtactg ctgatatagg cagataatgt
    11401 ttcgtttaat tcttttaccg tttggcgtaa ctctttacga atcgctttcg gaaagaagtg
    11461 agacaacgca tcaataaatt tgtgcccatc cttgaacata aatagcaaaa taaatggaac
    11521 ggtaaataac aaaataaaaa attgcatcat aaatgaaatg atttttgaga cacttgcaga
    11581 taaactgact aatatgatat tgaacaaatt actaagagat aaatttgctg atgctaattg
    11641 ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc aattcattta accatttgct
    11701 tgtttcatct gcatacattg gcaagctctg ggtcaattga attgtctgtt ccattaactg
    11761 aggcactaca ttcatgacag ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa
    11821 aaaaccgaac actctcggga tccgatgctt ttcgagaaaa acaacgattg gatcgaacat
    11881 ataatagata aaaccagcaa caagcaatgg taaaaaaatt gccgaaagca ttttcaatat
    11941 tggactaaaa atattcggca tttgtaaaat aaagtacacg ccgatgatcg tgagtactag
    12001 ctccactgtc cagaaaaata attttgattg tttaaaacgt gattgcatac atctcctcca
    12061 aggtcctttg atacttctat tctatcattt aacaaagagt tacgcgctga gattttcatt
    12121 tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag tagacgagta cttaaacaaa
    12181 aacacggttt ggagtagtga caaaaattat actgcagtcc ctttttccaa ataaacggtg
    12241 ttcaaaagct tgcatcttcg taataagccc agccaaacga aaaatataag gaactatttt
    12301 tatttggctg ggcttattac ttgatgtagg acatctattt tacaacctca gatcatttat
    12361 tgcgcaatac tgactaaatg caactttgtg atatttttgt gtacggacaa ctcctcgatc
    12421 acatcggcat aagtgagccc ttttggaaga tcaatcgtat agatattttt gtacactcgg
    12481 tagtcatcaa cgaattttac gtcaaaattc acatcttcga ttacgatatt tttctcttca
    12541 aaataatcgg taagaaaatc tttggtttct tttctatgga taaattggat ttctaacttt
    12601 ttagtcgtag gtacatggat cacgcgtttc actactgtga gtgcaatagc aatcccgata
    12661 aaactgatga tcgcaatgga atagtaaccc atgccgatgg cgatccctaa tccagcaatt
    12721 gcccataaag aagcggcagt cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt
    12781 cccgcgccta agaaaccgac accactcacc acttgagcaa tcagccttgc ttcatctgaa
    12841 cgaatcgttc cagcaagctg tggatactct aaagcatctc ttaatgcacc actagcgatc
    12901 tcaacttgga tcaatgcaat gatacatgct cccatacaaa ctaaaatatg cgtacgcata
    12961 cctgctggac gatttttata ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg
    13021 atccctaaac gtaaaataat ctctggaatt gtcaatacca tatcattcat ctaaacaccc
    13081 tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt
    13141 acgaaaaaag gactgtgaca aaatttttgt catagtcctt tttatgaata aacggtgttc
    13201 aatcagctga ccctcggtat tactcaacaa acggcaaaat atggggagct atttttcctt
    13261 tgtcttctct aatactcagg tcaaaacgct gatttcacaa cctctttagc ctactattga
    13321 aaattacaac ttacttgctg cttcttcatc gatgatcaca acaacattgt cgtgattttg
    13381 caatacactt gctggcaaat gattcgttac aggtccttca atcatccctt tgattgcatc
    13441 agctttgctt tcgccaaatg ccatcaagac gatttcttta cttttcataa ttgaaccgat
    13501 tcccattgaa acagcttttg ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc
    13561 attgattgtt gattctgtta agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg
    13621 ttcattgaaa ccgatatgtc cattttgccc gattcctaag atttggatat cgattggatg
    13681 tgcatcgatg atttgatcat actttttgca tgctgcttcc agatcttctg cttttccatc
    13741 tggtacaaac gtttctttga atggtttttt atcaaataat tggacatcca tgaaatgacg
    13801 gtagctttgt tcatctgaac cgcctagtcc aacgtactca tctaagttta cagaagtcat
    13861 ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc
    13921 tgttgccaat cctaatactt ttgcaccttt gttcatacct tctttgatga tttcaaatgc
    13981 tttttttccg ccttcttcag cgttttttac acgaatgatt tccatagtga ttgctccttt
    14041 atattttggt atagcccaat tatagcattc tttttttctt tttacaataa ttagtataga
    14101 ccaaattgca tttttttcat tttcttgaca ataaatagga atccgtgtta ggtttcataa
    14161 gatggttcaa tcaaacaatc catgttgcat gaaaaccaag caagtgattc actgtggacg
    14221 ctttatgtct gcagtttttt tatgaaaaag gagttattat gagtatactg acattaaaca
    14281 acattaccca gcaatttggc gacaaaatcc tctatgaagg tgtcgatctg caattaaatg
    14341 ctggcgaaca tcttgggctg attggacaaa acggagccgg taaaagtaca ttgattaaaa
    14401 tcatcactgg tgagatctta ccagatgacg gacagatcca ttggcaaaaa aatattcaca
    14461 aaggttacct cgatcaatat gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa
    14521 aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat tgcttttctt taccaaacat
    14581 atagcgagac cttcgatgat tccttgctgg aaaaagctgg gaaactccaa acggaattag
    14641 accaaggagt gttttatcaa atcgacacct tagttgctga gatgagcagc gggttgggga
    14701 tcgatgtctt aggtttagat tcactgttga aaaatctaag tggtggacaa cgttcaaagg
    14761 tgattttagc aaaactactt ttagaaaacc cagatgttct gatcttagat gaaccaacca
    14821 atcacttaga tgatcagcat atccaatggc tgacgcagtt tcttcaggat tttgacggtg
    14881 cgtatatcgt gatttcccat gaccaagaat ttcttgatca gatcaccacc catatcgcag
    14941 acatcgagtt tggaaagatt actaagtata ctggccatct aaaacaagca ttgaaacaaa
    15001 aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa
    15061 agaccgaagc ttatattcga aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc
    15121 gacaaaaaca attagataaa atcgaacggc tgacgcctcc agcttcagca agtaaagctg
    15181 tctttgattt tccattctcg cctattgtca cgacattagc agtggaaaca actgatttag
    15241 tcatcggata tgatcgaccg ctacttgcac ccatcaattt gaccatccgc tttggtgaaa
    15301 atattgccat tcgaggcttc aacgggattg gaaaatctac tttattaaaa acactgatcg
    15361 gtgagattga aaaaataagt ggcgatttcc acttcccaga taacacaaaa atcaattact
    15421 tttcacaaga tctgtactgg gaaaatccat tagaaacacc actacaatat ttaagcagtc
    15481 aatttccaaa agcgactatc aaagaattac gtcgccaact agctaaagca gggttagtta
    15541 atcaattagc aagtgaacct ctcactacat taagtggtgg ggaacaaact aaggtgaaac
    15601 ttgcacagtt gacaatgaac caaggtaatt tactgattct cgatgaaccg acgaaccata
    15661 tcgatcaaga aacgaaagaa agcttacaag acgggataca gaaatttcca ggaacagtaa
    15721 tcatcgtctc tcatgaacaa gaattttatc aagatcttgt gcaacgagtc attgagattg
    15781 aaccaaaata aacgtttgat tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc
    15841 acaactcctt cttctgaata aatggtggcc aattagctga ccttcggtat tagctcaaaa
    15901 aacggcaaaa tatgaggagc tatttttatt tggctattct tattacttga tgcagaacag
    15961 cttttcacaa cctcttttcg tctatttttt attttcgaga tttctctttc ctttaaaaac
    16021 aactgtttga aagaagaacg cacatccttg aaaaacaagc ccgctaaccg ttacacctaa
    16081 ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa
    16141 gtacataaac ataaata
//
LOCUS       DAZKSU010000061        16105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000061 DAZKSU010000000
VERSION     DAZKSU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.061"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..362
                     /locus_tag="ACG8Z3_002004"
     CDS             <1..362
                     /locus_tag="ACG8Z3_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0824310.1"
                     /translation="TDTTSTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSST
                     TSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVIL
                     LVIGLRITFMSYKKSKR"
     gene            complement(517..1503)
                     /locus_tag="ACG8Z3_002005"
     CDS             complement(517..1503)
                     /locus_tag="ACG8Z3_002005"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0824311.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1526..2092)
                     /locus_tag="ACG8Z3_002006"
     CDS             complement(1526..2092)
                     /locus_tag="ACG8Z3_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0824312.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2106..2522)
                     /locus_tag="ACG8Z3_002007"
     CDS             complement(2106..2522)
                     /locus_tag="ACG8Z3_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0824313.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(2979..4913)
                     /locus_tag="ACG8Z3_002008"
     CDS             complement(2979..4913)
                     /locus_tag="ACG8Z3_002008"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0824314.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5215..6150
                     /gene="pplA"
                     /locus_tag="ACG8Z3_002009"
     CDS             5215..6150
                     /gene="pplA"
                     /locus_tag="ACG8Z3_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0824315.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6240..7343
                     /locus_tag="ACG8Z3_002010"
     CDS             6240..7343
                     /locus_tag="ACG8Z3_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0824316.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7356..8309
                     /gene="menA"
                     /locus_tag="ACG8Z3_002011"
     CDS             7356..8309
                     /gene="menA"
                     /locus_tag="ACG8Z3_002011"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0824317.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9123..10469)
                     /gene="gor"
                     /locus_tag="ACG8Z3_002012"
     CDS             complement(9123..10469)
                     /gene="gor"
                     /locus_tag="ACG8Z3_002012"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0824318.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10603..11856)
                     /locus_tag="ACG8Z3_002013"
     CDS             complement(10603..11856)
                     /locus_tag="ACG8Z3_002013"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0824319.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11862..12785)
                     /locus_tag="ACG8Z3_002014"
     CDS             complement(11862..12785)
                     /locus_tag="ACG8Z3_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0824320.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13006..15498)
                     /locus_tag="ACG8Z3_002015"
     CDS             complement(13006..15498)
                     /locus_tag="ACG8Z3_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0824321.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15505..15972)
                     /locus_tag="ACG8Z3_002016"
     CDS             complement(15505..15972)
                     /locus_tag="ACG8Z3_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0824322.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4727 a         3360 c         2865 g         5153 t
ORIGIN      
        1 gtacagatac tacctctacc acaagtgata gcacggacac gtcagcttca agtgacagta
       61 cagatacatc ctctaccaca agtgatagct cagattcatc aacttcaagc gatagtacag
      121 acacgtcgtc taccacaagc gatacacgtc atgcaagtgt ctccttctca aaaacaagtt
      181 cggataacag gacgaacttt aacttaacta ataaaacaag caatgacaat ggaaacaaat
      241 caaaaagagc acttcctaaa actggaagtc aatcgaataa ttggataaca ctcgcaggcg
      301 ttatattact tgtaatcggt ttaagaatca cttttatgag ttataagaaa tcgaaaaggt
      361 aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg gacgttgctc tttatttttg
      421 ctttttatca taaatacaag aactacaatt caatttgtca aaaaacacgt agatttcctt
      481 ctgagaaatt tatttacgta tcgaaccttt ccctattcag tctttgcgat ccaaaatcag
      541 ttgggtaagg gaaaagagat tctcttttgt tcgaagagag gtggcaggta atttctttat
      601 ttccttcaaa gccttttctg tgtaagaggc cgctaacttc ctagcttctt ccacgcctcc
      661 tgtatgttca acgattttgc gtacggcgtc gacatcttct tctgtcattg ccgctttctt
      721 ctctaaataa ggtagtagct ttggtttgtt tttttgcaaa gagtagatca acggtaaaga
      781 atagacccct tgacgcatgt cttctagtac aggtttgccg atttcctggc tctcttttgt
      841 ataatctaaa atatcatcga taatctgaaa ggccatcccg atagaaagtc cgatatctcc
      901 tgcttttttt gcgaagcgct cgcttgttcc actttcaaaa gcgccaacag aacaacttaa
      961 ttggaacagt tcagctgtct tacctgaaat attttccaaa tattctttta cagaaagttc
     1021 ataattatag cgatcatcca tctgccctaa ttcgccattc aatacttttt ccatactgcg
     1081 agaattcaat tggagacttt tcaaggaagt ggagtaatca gacaatagtt taaaacagca
     1141 gacaaataaa tagtctcctg catagacaga aacgacatta ccaaactgac tacggatatt
     1201 aggctgacca cgtcgcagat ccgcttcatc aacgatatca tcatggatca gtgtggccgt
     1261 atggagcatt tcgatagaag ctgccaaagc gatggctttg tctcgatctt gctctggtcc
     1321 aaattgtgag aacaatagct gataggcagg acgcaacagc tttccgccgg catggatcgt
     1381 ttctttgatg gcttgttcga caggtttgtt tttcaggcgg acagtttctt ccatccgttt
     1441 tagtgtttct gtcaattctt tacttaaagc aggatagttt tcccacattg gatgtagctt
     1501 cataaaaaaa ctccttgtgt gacgattatt ttttggtaaa cattgggctc catttttgtt
     1561 tggttccctg ctcataatct ttttggaatc gttgaagtgt ctgcacatgt cgtaaaagat
     1621 aattcgctgc gatcccgatt gcaatccctg ataaaatccc taaaactgaa aggactggca
     1681 agtacaacat gactgtccat gattgtgcaa tgaaagaagc catcaaaagc tgtccgacat
     1741 tatgcatgaa accgccggcc gcactaattc cgatgatact gacacgtttt ggtcctaatt
     1801 gcttgaccaa taacattcca aaataactaa gcaaagcacc actcatacta taaaggaatg
     1861 tggaaatcgt tccgcctagc aaagtagtca ggaatagtcg tagccaaagt agtaagaagc
     1921 tgtcttgttt tttcatggta aaaatggcaa cgatcgtgat tagattggct aagcctagtt
     1981 ttgcaccagg cgcaaaagaa aaaggataag ggatcatatt ttccaataaa ccaatgatca
     2041 ctccttgtgc aacaagtagc gaaatataaa tcattttttg taatttactc atggtgtgtg
     2101 aaggtctaat aaatcagact gtcttcgtca cctccatctg ttgaacggac ttcgatcact
     2161 aatttatgtg gcaaacagac gatggtttcg ccatttttcg ttgcccagcc tcgacgtacg
     2221 cagatctggt catcgcaatc tgcttctttt atccgtactc gatcgccatc tatctcgatc
     2281 aagttataat ctccgtgttc atctttgtaa gtatatgtgt tgctctcttc tccagccatc
     2341 aaagggaatg ttttgatttc tgtgccatcc acacgcagta ccgcttcttt ttctgctgtt
     2401 gcggtgtttt gctgaagacc aaaaatcatg actggtaaaa aagaaagaac agtcaaaagc
     2461 aaaataataa aaacatccca aggtttgagc agactttttt cagcaaattc tttaaggctc
     2521 atttttgttc tcctttttcc agattaactc acccaatttt agcataaaaa caagaggttg
     2581 tgacacctgt gtcttgcttt gagtaataag aacgaagaat agaaaacagc tttccatgtt
     2641 ttctaattct ttgggcttat taccgagaag cagcaggtgg aacaccgttt ataacaagag
     2701 gttgtgacag aagtgggcaa cttcaagaaa taagaaggaa tttacgaaaa ttacttttca
     2761 agtttttgtg aaatttcggc ttatttttcg aagaagctac ttctggaaca ccgtttataa
     2821 caagaggttg taacagaggt tgtgacacct gtgtcttgct catggaggct tatctcgggc
     2881 atcttattcc tttaatttta cataaaaagg aggctgtgac ggtttgtctc agcccctttc
     2941 tattgaaatc ttgcattcaa ataacgttct ttatctattt atttcttgcc gtactgggaa
     3001 cttggtctac cgtaccacca attacctagt gtacgttgta gccatggaat tacccagcga
     3061 tccaacccaa tcgcacgtcc ggaaccgttc atcaatgcga aagcaacaaa aatgaaccaa
     3121 atgtttaccc agtaaaacat gcctgataag cagaacgtga tcaccaatac aatcgttgtc
     3181 gcactgcata gccatgtgaa taatccagca atcaatgcta atgccaaggc aatttcaaag
     3241 aataccatga atttttggaa gaacattgct acatcattgt ttggcatgat cacttgcatg
     3301 atctttgtga accagtctgg tgacttctca atcacagcca ttggttcttc accgtatgca
     3361 taactcaaac caaaatgtgc agcttgagtc gctgcttctc cggcacttgc ccctgctccg
     3421 ctggcagctg tggttgcatc accagcagca gaagcaccac tggcaacatc tgttacattg
     3481 tattcccatg tccaagggaa cacactgttt ccagtgaacc atgatccttc accaaaccaa
     3541 gtacttggtt tgaacacttc accattccca atgatttttt tggaagcttc aacgaaccaa
     3601 actaaaccat agaaaatacg aagtggcaca ctccataaga cattgccgta acgagaagaa
     3661 tgtcctcgag tcacgttgcg atcatctttg atatggaaaa actcatgcat taaatattgg
     3721 aacatataat aaccagaacg aatatcaaag aagtatttca aattcacgat atgtttcatt
     3781 acgatagcta aaaatccgct taaatggatc ttatcaaata ggttagctac cccccatttg
     3841 gcaccaatag aaaccataaa tccttgatag tttcctttaa atttatgctt ttctccgcct
     3901 ttgatcgaag ccacgatatt cgctgcggct gtgtgtcctg tttgttcagc agcttgaacg
     3961 atctgcggtg tcggtgttcc tgggaattct tcataaaaga ctaaatcacc aatgatatag
     4021 atgtttttat cttcgtagcc ttttgcctgc atatattcat tggcgatcaa ccgctgtccg
     4081 cgtgctgatt ctaatccaaa gtcagcagcg tcagaagttc ctttgacacc agctgtccaa
     4141 atcagtgtgt gagtcggaat gttgcgtccg tctttcaatt tgatatgatc agcagccact
     4201 tctacgattg gtgcattcaa taataattcc acgttgtgct tacgcaaata gcgttcagct
     4261 tttgcagcat catttcgatc caacatattc aagatcgtcg gcattgcctc tacgaccatc
     4321 agtgtgattt cgtttggatc taatttataa tctttggcta ggcgattttt ccagtcgatc
     4381 agttccccaa tcatttcaat accggtaaat ccagaaccac agacaacgaa agtcagcatg
     4441 gctttacgca gttcatcatc tggttccaat gcagcttttg ctactgtctt ctcaatatgt
     4501 tcccgaatcc ttacagaatc ttcaaatgac caaagggtga atccgtgtgc tttgacacca
     4561 ggtgtaccaa agtcattcgg ctctccgccc attccaagga tcagctgatc aaaaggatag
     4621 gaacctaatt tggtcttgac gatttttcgt tctttatcga taccgattac agtgtctgtg
     4681 acaagttgta cattcttttg acgagcaaac aaatgctgca gatcgtattg gattgcttct
     4741 ggttctacac gtccgccagc tacttcatgc aactcggtca tcatagtatg gtacgaatga
     4801 cgatcgatca gagtaattgc tacatcttct tttttcagtt tctttgccaa gtattttgta
     4861 gctgaaacac cggcatatcc tgctcctaca acgacaatat tttgctttgc cattgatcat
     4921 ccgctcctta tagactatct atataatgaa tatatgataa ttcttttagt ttgatactta
     4981 ttacacaatc tccatatatt atatagatgt tctgttcttt tgtctaactc cttttggatt
     5041 tttttcaaat tttaaaataa agcgtttttt cacgaaattt gctgaaagaa aacgtttgaa
     5101 atattacatg ggttatttat caaaaatagt tgacttatag gctaaaaaga ttagaatgta
     5161 taatgaatca caaagaaaaa ttgtggacta gaaatggaat ggtggggtat taaaatgaag
     5221 aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt cagctctagt gttaggcgct
     5281 tgcggcgcag atgacaacgg aagctcaaac tcttcttctg aatcatctac agcacaatct
     5341 tcgactgcaa aaagcacaac agaatcatct gcaaaagttg tagctggcgg agatttgcaa
     5401 gacggcacat ataaattaga agaaaaaaat tattcaaatg gttaccgtgc agtctttgaa
     5461 atggttgtaa aagacggcaa aatcactgaa tcaaaatatg ataacgtcaa tgaaaacggc
     5521 gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg aagcaaaatc tggcacaaac
     5581 ccagaaaaat acattcctga gttgaatgac caattcttaa aagcacaaag cgcaagcggt
     5641 gtggaagtcg taactggagc tactcactca tctgaaagct tccaaaacta cgcacaacaa
     5701 ttgatccaag ctgctcaagc tggtaacaca gatacaatcg aaatcgacaa tggtgctgat
     5761 ttgaaagacg gcacttacaa gcttgaagaa aagaactact ctaacggcta ccgtgtgcaa
     5821 ttcgaaatga cagttgctgg cggcaaagta actgaatcaa actttgatta tatcgacaaa
     5881 gacggtaaat caaaacaaga tgatacagaa tataatgaaa atatgaaagc aaaaagcggt
     5941 acagaaccaa aaacttacat ccctactctg aacgacgaat tcgtcaaagc aatgggcgaa
     6001 gaagatggct cacctgctaa tgtagaagtt gttacaggtg ctacacacag ctcacattca
     6061 ttcatcatgt acgcacaaca attagtgaat gcagctgaaa aaggcgacac tcaaacaatc
     6121 gaagtagata atatcgtaac agaaaaataa tcacataatc atactaataa aagttggagg
     6181 gtgggacgat agccagatta ggcagtcgtc tcacctttca aaatgaagag aggaaaaaga
     6241 tgcggaaaaa actactattt tccttaatca cactgggcat ctcttttatg gtgctaactg
     6301 gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc agcagctacc aagatattga
     6361 aagatccata ttctgatgaa cagttcttgt tagggactta cgttcgtatc cgtgtgtatg
     6421 atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg agtaaaagaa cttggtgata
     6481 aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt gaatgagcaa gctgggatca
     6541 aaccagtaaa agtttctgat gacgtctaca cgttagtcaa gcgggcctac gaatatagcc
     6601 aagattctca aggcggattc gatatggcga ttggtgcaat cacacaatta tggcgaatcg
     6661 gttttgatga cgccagaaaa ccttcccaag aagaaatcga tcaggcattg aaattagtgg
     6721 attatcataa gatcgaacta aatgacaaag aaaaaactgt ttatctaaaa gaaaaaggaa
     6781 tgatcatcga tttaggtgca atcgccaaag gttacattac ggacgaagta gtgaaagtct
     6841 tgaaaaaaca aggagtgacc actgccatcg ttgacttggg cggtaatgtc tatgtcttag
     6901 gccacagccc aagaggcgaa aaccaagact ggacagtcgg catccaagat ccgaacatgg
     6961 ctcgcggatc tgttttaggt tctattaaag aacggaacaa aacattagtt acctctggaa
     7021 tttacgagcg ttatttagaa gtagatggca aaaaatacca tcatttgttt gatccaaaaa
     7081 cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat cataacagat aaatcaattg
     7141 atggtgacgg gttgtctaca gcagtctttt caatgggtgt gaaaaaagga ctggaatacg
     7201 tcgaaagcga attgaataac gggacggaag cgatcttcgt gacaaaagac gataaagtat
     7261 atgtgaccga tccaatcaaa gacaccttca aattatcgga ggactcccat tacacgatgg
     7321 gtgaccgttc tgaactaaaa taaggagtac gaaccatgac actaaaaatc tttttagaag
     7381 tagttgaaat acgaacaaag gtggctagtg tatttccttt tgcggtaggg gtattattct
     7441 ctatcgcttt ttttcatgag atacattggc taaacacttt tttatttttt attggaatgc
     7501 tagtttttga tttagcgact accgctatca ataattacat ggattatgaa aaggctcatt
     7561 ctgaagtata taagtatgaa gaaaatgtca ttggccgcag aaatctatct cctgtattga
     7621 ttcgaaacat gattttagct atgattgcat ttgtttttgt tatcggcgtt attctaacga
     7681 tcaatacggg ttggctttta cttttgatgg gatttatctg ttgttttatt ggagtattct
     7741 atacattcgg accgattcct ttatctcgaa tgcctttagg ggaaattttc agtggggtga
     7801 ctatgggcct tggaattttt gccatggtcg tttatgtcaa tacattcggt tcagatgtct
     7861 atgatctgac agtcaacttt tcatctggac aatttgattt ggtaggaaat ctttgggcta
     7921 tagcagcaat cgtattagct tccttaccct tggtgtttac gattgccaat atcatgttgg
     7981 ctaataattt gcgggatctt gaccgtgata ttgaaaatca tcgctataca ctagtttttt
     8041 atatcggacg tcctgcggga acagtcttat tccagctatt gatgtatgct tgctatttcg
     8101 tgattttaat ggggttgttg agcggtatct atgaatggcc gatcttggtc accttcttga
     8161 ccttgccaat tgtgaagaag catttggatc aattcaagca aacgttaccc cagcctatca
     8221 gtttcagcta ttccatcaaa aatatggtct tattcaatgc cagttatgta ttggggctat
     8281 tactaacaat cattatgcaa gccatttaac agcatgaaga ggttgtgaaa aaactgccct
     8341 gcatcaagta ataagaacag ccaaataaaa atagctcctt atatttttcg tttggttggg
     8401 cttattaccg cagatgcaag cttttgaaca ccgtttatca ggaggttgtg aaaaagtcgc
     8461 ttagtttcga gtattaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     8521 tggcttaata ccgagaaact ggcttttgaa caccgtttat cagaggttgt aaaatcagtg
     8581 ttttgtcctg agtattagag aaacaaatgg aaaaatagtt cctcatattt tgccgttttt
     8641 tgagctaata ccgaaggaca gctgattgaa cactatttgt tcggaaaaag tgactaacta
     8701 tgacacaatt tttttcacag tccctttttt cacttgattt gattcgtacc agcgaacttg
     8761 acgagaactt tcttggtttg gtaactatat ggtattcttt taacatcctc gtttgcttct
     8821 tctaatttat ttatacaaag tcacactttt ttaaacatct gttcttcgat acatttctat
     8881 ttcttttaaa catcgatacc aatattttga acttaagagt gcataccaaa tggattcatt
     8941 tactcttcgt cagttacgaa gataatcaaa atctccaact tccctactta tttcgttttg
     9001 agaattatcg aagcaatctt acataaataa ctaaaaaaca aaaaaccaga aaatcagtat
     9061 actgattttc tggttttaca tggtttgttc aataaaaagc tgttggaact gttaatatta
     9121 aattatcgca tcgttacgaa ttcttccgct cctgttggat ggatggctac tgtattgtca
     9181 aagtcttctt tagttgcacc catcttgata gccacggcaa aaccttgcag catctcatcg
     9241 acaccaacac caatagcatg taagccgacg attctttctt cttttccgac acagatcaat
     9301 ttcatttcgc atttttggcg ataatcattt aatgcaaagt acattggggt aaagcgtgaa
     9361 cgataaactt tcaattcatt ttcgccatat ttttccaatg cttcttcttc tgtcaaacca
     9421 attgttgcaa ttggtggatg cgtaaagacg actgtcggaa caagtttata gtcaagataa
     9481 gaatctttct ttccattaaa taaacgttct gataaacgtc taccagctgc gattgcaaca
     9541 ggagtcagat ctaatttacc gatcacatca ccaacagcaa aaatatgttc atccgttgta
     9601 ttttggtatt tatctacttt gataaaccct tttttgtcca attccacatt tgtattttcc
     9661 agacctaatt tttcggtatt cggtacacgt ccaccggcaa aaagtacaca atcaccagtt
     9721 aaggtttcac cattttcaaa tagcaccgta tattcgtcat tgtttttagt aatttcttta
     9781 gcaatatgat gtgcgtaagt ttttattcca gcttcttggt acatttggat caaattgtcc
     9841 gaaagcattt tatcaaacgt tctaagtggt cgttcttttc gatatgccca catgacttca
     9901 ctacctaaac cacgaaatac acctgaaagt tcagccgcaa tgtatccagc acctaaaacg
     9961 attgctctct taggtaactc tgttaattca aaaaatccat ttgaatcaat tgcgtattct
    10021 ccaccaggaa tggtcatttt acttgcacgg cctccagtag cgatcaagat tttaggagca
    10081 cgataggttt caccgtttac ttcgatcgtt tgactatcga taaaagtagc gtaacctttt
    10141 atagcctcga catgattgct atctaatcct ctttgataag caccgtgtag aaaatcaata
    10201 tatttttctc gtctttctac tagaccggcg aaatcaacat tttttaaatc tgcttggata
    10261 ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg tacttgcttg ccacataact
    10321 tttttaggta cacaaccgac gttgacacat gttccgccca attcattcgc ttcaatcagc
    10381 aaaacttttg cgccatgcat ccctgctctg tttgctgaag caataccgcc agagccgcca
    10441 ccaattacga tgtaatcata tatttgcatt ttgttcctcc ttattgaaga gatcaatcaa
    10501 aacctttatt taacgatcct ttttctacag aaaacaagtg taacactttt cttgaatttt
    10561 gtatgcgaag atgcttaaaa cagggatata tcagcttttt ccttatttac gttttcggat
    10621 catatctcca ggtttcaccg gctgtgcgac tggcatagtt aaaatcatca ttggatttgg
    10681 tgctcggtca atggattcgc cttcttcatt atgcataact tcaacggttt gactgaaatg
    10741 actgaatcct ggaccataaa attcgatttc atctcctaca gaaaacaagt ttctctgacg
    10801 tatcgtagca atctgtgttg cctcatcata agataatact tcaccgacaa atttatatac
    10861 tgggatttta cgacgttcac cgaataattg ttcgttttca gttggtgtat tgtagtaaaa
    10921 acctgtagct aattctcgtt gtgctacttt ccataactcg tcgatccatt cttgcttgca
    10981 gacatagttt tcaggatctt ccatataact gtctacagcc gctttataga catttgacac
    11041 cgtagataca taatggatcg atttcattct tccttcgatc ttgaagctgt ctacgccgtt
    11101 ttcaatcaat tctggaatat gttcgatcat tgacatatcc actgcactca tcgagaattc
    11161 ttcttccact tcgcctgtat cagttaaact gtttcgttct tgaccaaaag gcatatcgaa
    11221 caatccatac ttccagcgac aagattgaga acatccgcca cggtttgcat cccgcatcga
    11281 catatggttg gacaatgtgc atcgaccaga ataagagata cacatggcac catggataaa
    11341 tgcttcgatt tcggtatccg tatttttacg aatctctgcc acttcctcca ttgaaacttc
    11401 acgagctaaa acgacacgtt ccaacccttc ttctttccag aattccaatg tctcaaaatt
    11461 agttgctgaa gcttgagtgg ataaatggat cggcagacct ggggcttctg tcgcacaaat
    11521 ttcgatcaaa gctggatcag aaacaatcac tgcagaaata ccgacatcac gcaaactgcg
    11581 gaaaaattcc cctgctccat cttcatttcc ttcatgggtg accatattag cagcaacgta
    11641 taccttcgca tcatgttctt ttgcaaaagc aactccttca gccatttctt cataagtaaa
    11701 attacctgca cggcttctta aaccatatgc attcccgcca atatatactg catctgctcc
    11761 ataatggatc gcggttttta acttttccag tgttcctgct ggcgctagga tttctggtcg
    11821 tttcaatttt ctttttactg tcatttcttt catcatactc cttatttgat agcagaagga
    11881 tcaatataaa agaatcctgt atctaattca cggttttcag gatgcaattt actgatttcg
    11941 ctagtcattt tttccgcttt ttcttgatcc cagctacctt ctaaaatttg tttttttgct
    12001 tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg gagtaaataa tccgtccaat
    12061 ttccatgtac agtaatgatg ctgataaagt tcattcaatt cattcgagag attcagatca
    12121 ttgctggcaa aaatatgtgt gccatgacta tcttcataaa tagaataatg tgtctcttct
    12181 tttttaggtt ccgagataaa aagacctctt tcacgatctt tttgttcgtc ttgtttcgta
    12241 tagttatagt aattctgtag cagtggacgt ttggattgat ggatgcatgt agcaccatac
    12301 acaagggttt ctacaggaat atcgagtttt tcttccatcg ccttcatctc ttcaaaagga
    12361 acttcacgag caagtactgc tccaactgcg ccttttttcg cccagaaatt gatttgacgt
    12421 gcgctcgtta ctaatgtttc agcatcataa atatacggta tacgcaattc aggatttttt
    12481 ttcatcgtat aaataatacc tgtatcacca actgtgatca catctacgtt tatttctttt
    12541 aaaaaagcca aatattcagg tacatttttc attttttcag gatgaatgat cccatttaca
    12601 gcaatattcg cttttttacc atacttgtgt gctaattcta ccaattgtcg ctgttcttct
    12661 cgtgtaaatg aagcaggcag acgcaatcca aattgctctt caccgaaata tagtgtatct
    12721 gcacctgcag caagcaactg ttctgcttgt tcaatagttt ctactgtggt tatgagttcg
    12781 atcataattt tctcctccta caacttcata atagtagcat agctctaaaa actttccaat
    12841 acttcttcta tttaaagtcg gatttttgaa aattatttca caaaaaatca aaataaagtt
    12901 cgatatattc tgtccaaata taaaaagaga ttgtgacaaa aatcttgtca caacctctta
    12961 atcaagtctt actattataa aaattcagct acacaggcca agtaactata gggaacctac
    13021 tgtttccttt ttaggtccgc gaacattcaa tgtgatctta ccttttgaag cacctactgt
    13081 tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc tcgctcaatc gatcttcaat
    13141 ttctttttga agtgcgcgac ggattggacg tgcaccatat tcaggatcaa atccagcttt
    13201 cccgatcaca tcgattgcag atggtgtgat tttcacttga atatcttgtt ctttcaatcg
    13261 tttgataatc gctttgctca tgatttggac gattgtatgg atttcatctt tactcaatga
    13321 atggaagacg acagtctcat cgattcgatt caagaattca ggtcggaaag ctttctttaa
    13381 ctcttctaag atacgttttt gcatggcttt gtgatcttta gtaacatctt ggacattgaa
    13441 tccgacattt ttttcttctc gaatttgtgt cgctccgata tttgaagtca taatcatgat
    13501 tgtatttcta aagtccactt ttctaccttt agaatctgtt aaatgaccat catccaatac
    13561 ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc acttcatcta agagaatcac
    13621 tgaatatggt ttttgacgaa ttttttctgt caactgtcca ccttcatcat accctacgta
    13681 tcctggagga gacccgatta atcggcttgt actgtatttt tccataaatt ctgacatatc
    13741 aacacggatc agggcatctt cacttccaaa catgacttct gaaagtgctt tggccaattc
    13801 tgtttttccg acaccagttg gtccaaggaa catgaatgaa ccaattggac gatcaggatc
    13861 tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact gctttgacag cctcatcttg
    13921 accaacgaca cgttcatgca gaagtccttc cagttctaac aagcgttcgc tttctttttt
    13981 ctccaactgc tgtagaggga caccagtcca ctccgataca actgtagcaa catcttcttc
    14041 tgttacacga tcagaataac cttgctcctg ttttgcttca attgcagaaa ctttttgcag
    14101 ctttttacgt agtgcttttt cttgtctgcg taattgagca gcattttcaa agtcttgttt
    14161 ttggatcgct gcttcctttt cttgtactag ttcatcgatt tctagtttga tcacagttga
    14221 ttttgttaag tgatcggttt gatccaaacg gactttcgct gctgattcat cgatcaaatc
    14281 aattgcttta tcgggtagtt gacgagaagt gatataacga acagaaagat tgactgctgc
    14341 tcgtacagct tcatctgtga tctccactcc atggtgctct tcatatcttg tacgtaatcc
    14401 ttgaagaatc acttctgctt cttcaggagt tggctcatct acttgaatgc gagcaaaacg
    14461 tcgctctaat gcagaatctt tttcgatata tttttgatat tcatctaggg tcgttgctcc
    14521 gatcgtttgt aattctcctc gagctaacgc cggtttcaag atattcgaag catcaattgc
    14581 tccttctgct ccacctgcac caattaaggt atgcaattca tcaatgaata agattacttg
    14641 accatcatta tagatttcgt cgatgacctt tttcattcga tcttcaaatt caccgcgata
    14701 tttcgtacca gcaaccaaag atcccatatc aagcatcatc aggcgctttt cttgcatgtc
    14761 ttctggcact tcacctaaaa tgattttttg agctaaccct tcagcaatgg ctgttttgcc
    14821 tacaccaggt tcacccacca gtacagggtt attctttgtc cgtcggctaa gaatctggat
    14881 aagccgtttc acttctctcg aacgaccaac aactgggtcc agcttgtttt cttttgctaa
    14941 tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt ccttgcggtg tttgccgttt
    15001 atcttgtaca tttacatttc tacgacgatt cattcctttt ccagcagaat caccagctag
    15061 ccccattttt tgtttcagaa cttgacgggc ttttgaaagg ctgataccta agttgatcat
    15121 gattcgagta gccatgatac tttcatcttt taataagctc atcaaaatat gttctgttcc
    15181 aactttttga gaaccagttt tcttcacttc gctgctagct aaagcaaaaa cttgtttcat
    15241 tcttggtgaa tatggaagaa agactccttt agggtaagaa cggataccac cataaccaat
    15301 cagatgttca atttcttcat aaatttccgt ttcattcaaa tccatttcac gtaatacttt
    15361 tccagcaatt ccattttgct cgaccactaa ggctagtaaa acatgttctg aactaactga
    15421 ttgatgtttc aaccgttttg cttcttcttg tgcaatttct aatactgctt gggctctttc
    15481 agtaaaaagt tcttccatct aatctcactt cccatcttca taacttaaac gttccagaaa
    15541 tgctcggaga atccgagcac gtgtatgatc ttcatattca ttgaaattca atgtgttttt
    15601 tgcaattgca ctcaacatca agtttccttc tttttttgta attatttggt cttcataaag
    15661 tttttgaata atagcaaaag catttttctc actgattgta tcatcaaata actgatttat
    15721 ctgctcaagt aattgttttt tatctgaaat ccttactttt gcaattcgta tataaccacc
    15781 accaccacgt ttgctttcta ctagataacc acgttggatg gtaaaacggg tattaatcac
    15841 atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc atttcaatcc ttcgaatctc
    15901 gatcatttca ctattttcca gaattttctt tatatatgat tcaattaaat ccgaggtatt
    15961 ctgatgtgcc atcttattca ttccctttga ctaatattga ccttaattat aacgaatttg
    16021 ataatagaaa gaaagaagaa cgctttttgt taaataaatc ctttagaatc aacaaaaaaa
    16081 aatacggaat atactattcc gtatc
//
LOCUS       DAZKSU010000062        15244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000062 DAZKSU010000000
VERSION     DAZKSU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.062"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG8Z3_002017"
     CDS             168..362
                     /locus_tag="ACG8Z3_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824323.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG8Z3_002018"
     CDS             631..930
                     /locus_tag="ACG8Z3_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824324.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG8Z3_002019"
     CDS             complement(1027..1461)
                     /locus_tag="ACG8Z3_002019"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0824325.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG8Z3_002020"
     CDS             complement(1693..2403)
                     /locus_tag="ACG8Z3_002020"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0824326.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG8Z3_002021"
     CDS             complement(2393..3253)
                     /locus_tag="ACG8Z3_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0824327.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG8Z3_002022"
     CDS             complement(3258..3896)
                     /locus_tag="ACG8Z3_002022"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0824328.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG8Z3_002023"
     CDS             complement(3911..4210)
                     /locus_tag="ACG8Z3_002023"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0824329.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG8Z3_002024"
     CDS             complement(4253..5737)
                     /locus_tag="ACG8Z3_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0824330.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG8Z3_002025"
     CDS             complement(5757..6218)
                     /locus_tag="ACG8Z3_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0824331.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8Z3_002026"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG8Z3_002026"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0824332.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG8Z3_002027"
     CDS             7521..8291
                     /locus_tag="ACG8Z3_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0824333.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG8Z3_002028"
     CDS             complement(8564..9391)
                     /locus_tag="ACG8Z3_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824334.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="ACG8Z3_002029"
     CDS             9959..11194
                     /locus_tag="ACG8Z3_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0824335.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="ACG8Z3_002030"
     CDS             complement(12193..12453)
                     /locus_tag="ACG8Z3_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0824336.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVTHLNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="ACG8Z3_002031"
     CDS             complement(12480..13781)
                     /locus_tag="ACG8Z3_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0824337.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="ACG8Z3_002032"
     CDS             complement(14073..14531)
                     /locus_tag="ACG8Z3_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0824338.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..>15244)
                     /locus_tag="ACG8Z3_002033"
     CDS             complement(14544..>15244)
                     /locus_tag="ACG8Z3_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0824339.1"
                     /translation="KELQREIMPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITK
                     NFPSNIPNNYLEYYLYPDMVVEHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVL
                     NFNFGEHGQYIVDIAISLLNDDRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEP
                     ITLRKPISDFHKGLMEAQVAVEKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILD
                     EMIKVNKKYWPALK"
BASE COUNT         4876 a         3105 c         2365 g         4898 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcaaatgcgt
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacgac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttat
//
LOCUS       DAZKSU010000063        15236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000063 DAZKSU010000000
VERSION     DAZKSU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.063"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..939
                     /locus_tag="ACG8Z3_002034"
                     /pseudo
     CDS             6..939
                     /locus_tag="ACG8Z3_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(943..1662)
                     /locus_tag="ACG8Z3_002035"
                     /pseudo
     CDS             complement(943..1662)
                     /locus_tag="ACG8Z3_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(1687..1872)
                     /locus_tag="ACG8Z3_002036"
     CDS             complement(1687..1872)
                     /locus_tag="ACG8Z3_002036"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0824340.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            2099..3372
                     /locus_tag="ACG8Z3_002037"
                     /pseudo
     CDS             2099..3372
                     /locus_tag="ACG8Z3_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3479..4510)
                     /locus_tag="ACG8Z3_002038"
     CDS             complement(3479..4510)
                     /locus_tag="ACG8Z3_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824341.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(4517..5347)
                     /locus_tag="ACG8Z3_002039"
     CDS             complement(4517..5347)
                     /locus_tag="ACG8Z3_002039"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824342.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5344..6159)
                     /locus_tag="ACG8Z3_002040"
     CDS             complement(5344..6159)
                     /locus_tag="ACG8Z3_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824343.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6156..6980)
                     /locus_tag="ACG8Z3_002041"
     CDS             complement(6156..6980)
                     /locus_tag="ACG8Z3_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0824344.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(6970..8070)
                     /locus_tag="ACG8Z3_002042"
     CDS             complement(6970..8070)
                     /locus_tag="ACG8Z3_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0824345.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8248..9033
                     /locus_tag="ACG8Z3_002043"
     CDS             8248..9033
                     /locus_tag="ACG8Z3_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0824346.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9066..9449
                     /locus_tag="ACG8Z3_002044"
     CDS             9066..9449
                     /locus_tag="ACG8Z3_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0824347.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            9525..9656
                     /locus_tag="ACG8Z3_002045"
     CDS             9525..9656
                     /locus_tag="ACG8Z3_002045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0824348.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            9625..9861
                     /locus_tag="ACG8Z3_002046"
     CDS             9625..9861
                     /locus_tag="ACG8Z3_002046"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0824349.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            9873..9959
                     /locus_tag="ACG8Z3_002047"
                     /pseudo
     CDS             9873..9959
                     /locus_tag="ACG8Z3_002047"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(9939..10910)
                     /locus_tag="ACG8Z3_002048"
     CDS             complement(9939..10910)
                     /locus_tag="ACG8Z3_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0824350.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            11544..13004
                     /locus_tag="ACG8Z3_002049"
                     /pseudo
     CDS             11544..13004
                     /locus_tag="ACG8Z3_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12927..13340)
                     /locus_tag="ACG8Z3_002050"
     CDS             complement(12927..13340)
                     /locus_tag="ACG8Z3_002050"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0824351.1"
                     /translation="MARKKGNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(13470..14846)
                     /locus_tag="ACG8Z3_002051"
     CDS             complement(13470..14846)
                     /locus_tag="ACG8Z3_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824352.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(14846..>15236)
                     /locus_tag="ACG8Z3_002052"
     CDS             complement(14846..>15236)
                     /locus_tag="ACG8Z3_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0824353.1"
                     /translation="NLIDYLSPIENIRLVNKSADESILFELGLDKKQIKRNVMKLSGG
                     QQQRVAIARALVSDAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKE
                     VADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT         4596 a         3339 c         2782 g         4519 t
ORIGIN      
        1 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
       61 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggga
      121 gattggatga ctcaccttcc cattgtgaac actgtggatc gattcgtatt atccgcaacg
      181 gttcctatac gacacgtact cagatgttga aagtcaaaga aaagttgact attttagaat
      241 taaaacgtac ccgttttcta tgctatgatt gtgggcagac cttttctgcg aaaacggact
      301 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggcgattctc atagaactgt
      361 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
      421 cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg
      481 tgttgtgtat ggatgagttc aaatcaatga agtctgtttc tagttccatg agttttattt
      541 gtgttgatgg gacaacccat cagctattta cgatccttga agatcgcaga ctttacaaac
      601 taactcaata cttcctgcac tttcctagaa aagcccgttt aaaggtcaag tacctagaat
      661 gccagctatt gtcagctgct taaaacggtc tttccaaatg cggagatcgt aactgatcgc
      721 tttcatattg tccaacatat caatcgcagc ttcaatcaat tgcgtatcca gatcatgaat
      781 cgttttcgag cctcgcactc tgaaaatcag aagaagtacc gtcggctcaa gcgatattgg
      841 aaactcttac tcaaagattc cacgacattg gagccgctca aacggcatta tcatcgactg
      901 tttaaacgac caattagtca aacagaaatc gtcgatgaac cgttgtgcac aatggtgtcc
      961 tgaacgtaca gcgacacctt ccatatccag agcagtcgcc acatcacggg gatggatgcc
     1021 atccaaatta aaagcaacca cgccagtatg atgttcagga tcttgcggtc cgtaaatggt
     1081 gataccgccg atcgcttgta atttaggtaa aacgtaggca accagttctt gctcataacg
     1141 atgaatattc tccataccaa tctcattcaa gtaatcgatt gctcctgcaa tgttcggagt
     1201 accagcttca aatttccatg gcagctcttt ccatgaacta tcgtaaagat tgacaaaatc
     1261 tatcatttca ccgccgaatt cgactggctc catttgttct aacaattgac gtttgccata
     1321 taatacaccg attcctgtcg gcccgcacat tttatgcccg ctaaaggcga aaaaatctgc
     1381 atccaacgct tgtacatcaa ctgtcatatg cggtgctgct tgggcaccga ctggactatc
     1441 ctcaactaca acagcctgtt caaccctcag ccgtttcaag cacaacaaat aaccttctgg
     1501 atctggtttg ccgacctgaa gatcatcgat cgtcacaaag gaatcaattt tttccttaat
     1561 catcggatgt gcttcaaaaa aatagtctag ctcccgcagt tcagaattga gactactccg
     1621 attttccagt gctgctgatc cgctgctcaa aatgttcagc atccactggt ctcccaaccg
     1681 cttgacttat cccttcataa aaggacagtt ctgctgccgt cccaacacct tgttgatagt
     1741 ctgcaaaagg aattttatag ccgaactgct ccagtaaata ggcttcagta aatagtgatg
     1801 aatactctcg taaaacatcc attcactgtc aatcatcgtc ccatcaaaat caaaaataag
     1861 tgctttggtc atcttctttc ccccttaccc aaaacaagca aaagctgtgt cctgctctct
     1921 ctacagtatc agcataaaaa ctgccgtctg aaaagtcaaa ggtgaaagaa atgtggcgct
     1981 ataatttcta ggactgatag taaatcattc tatcagtcct tttgagcata aaaaaacaaa
     2041 acatacagaa gctccgttag aattgaatca ccacaacaca atcaaaggag cgttctgtat
     2101 gcctgatctt catcttatca aactattact aggaatcaaa gatggaaaca ttcaaatcac
     2161 tcatgtcgag aagaaaaaaa tacattcaat caacagtatc gttgtttctg ccacgctcat
     2221 taaaacgatt cagccctgtc ctcagtgtaa acaggtgaat caacatgggc tgatcgttaa
     2281 gaacggaaag aaaaaatcgc tggtccttct caataagtgt gcgaatcaac ggacttatct
     2341 tgctttggca aagcaacggt acctctgtcg tggctgtcac acctatttta cggcgaatac
     2401 ctacattgtt gaaaaaaact gttttatcgc caaacaagta cattataaaa tattggagga
     2461 attgactgaa aaacaagcaa tgacgacgat ttctaaacat tgcggcgttt cttggtcaac
     2521 cgtttcgcga acactcgctt ctctgctccc catgacaaac gtgaaaataa attggttgcc
     2581 tcgctgttca ttggtagatg aatttcgttc attgaaaaat caagtcggtc cctattcttt
     2641 ttcttgtatg gatggcgata ctgggaaatt actggatatt cttccttcac gtaagaagaa
     2701 agatctcatt tcttatttta tgcaatttga acgacgtgca cgtttaaacg taaaaatact
     2761 cgtaacagat atgaacgcct cttatgcttt gctgataaaa gagtgctttc ctcatgccaa
     2821 actcgttgtg gatcgctttc acatcgtcaa gcacctcatt cgcagtttta aggatatttg
     2881 actatgagtc atgaaaagct tcgaccgaaa cgatccaata caagccaaac actatcgaca
     2941 agtgaaggct ttatctcgac ttctgatcaa acgacaggac acgcttgttt atgataaatg
     3001 gacaaaatgg cgtaattttg gttgggccta tttaaccgaa agtgaagtcg tagaacgtct
     3061 actttctact tctgatgaac tacgaatagc ttatgcttac tatcaagaaa ttcttcaagc
     3121 tttttatgac aaagaagcgg acatcttctt tcagcttgta aaaacaatgc ccaagtcggt
     3181 tccaagagaa ctgcatcata taaaaaaagc gtttataaac tatgaatccg gcattcgttt
     3241 agccttagag ttgccttact caaatggcaa aatagaaaat ctgcataccc acatcaaagc
     3301 attgaaacga atagcttacg ggttaggatg agaaccagaa ttttcttaat caatcatctg
     3361 atcacttatt aaacataaaa aaacatctaa agcagaaaat caattctacc ttagatgttc
     3421 ttatatcact ctatcagtcc tatttgacaa agagccagaa atgtttgccg actatacact
     3481 agctttgttg tgcaaaatgc tgagtatcaa tttccacagg gattgctaga atatctttca
     3541 gctgaatgta taaggcatcg ctaattttct cttcccgatc gttcaaccga tccactaaaa
     3601 tttctttgcc ctccagcgtt tgaaaaacat accgttgaat gttgcctaaa ataaatgatt
     3661 ccttccatag aaccggaatc ttaaccccct gaggaaccgg ttcaaattga atcagctccg
     3721 gtcttaaata ataaatgcag cgattgccct tagcggaaaa ctcgtgggta tactgattca
     3781 gctcatacca ttcgaaacga ttatagttgc cgataaattc cgcaacaaat cggctctgag
     3841 gattctgata caattccttc gaggtggaaa cctgagccac ccgcccagct tccatcacaa
     3901 aaatccgatc actgattcgc atcgcctctt cttggtcatg agtcacaaaa atcatcgtca
     3961 tccctagcga ttgctggata gcgcgcaaat cccgctgcag ctgttttcta attcgggcat
     4021 ccaaagcact taatggctca tccagcagca ggactttcgg ttcagtgacc aatgagcgag
     4081 ccagtgccac ccgttgtcgt tgaccgccag aaagaaactg gacattttta tctgcctgct
     4141 ccgtcatatc caccaatgat agcatctcaa aaaccttttc ctgaatcacc tcaggactca
     4201 gcttctttac cttcaaacca aaggctacat tttcaaaaac agtcatagtt ggaaacaatg
     4261 catactgttg aaaaacaaag ccgatattgc gatccttcgt aggcttatcg gcaatattct
     4321 cattgtccag cacaatctca ccgctctttg gttgttccaa tcccgtgata cagcgcagca
     4381 acgtagactt tccgcacccg ctgggaccta gtaaagtaac cagctcaccc ttttcaatct
     4441 gaatttcatc aatagctaaa atctgttttt catcaaaaaa cagattgaga tcatgtattt
     4501 ctaaaaacat tttccgtcac aactccttta agggcttctt tttcgactgc ttctcttccc
     4561 tcgtctgctt aagggacagc tgttgctttt gtcgataagt cagcataaag gtagttaaag
     4621 caattcccac cataaacagc aaatacaaaa ccatcacagc gctagccaaa tggccgtttt
     4681 cgttcatccg ccgcagcata tagatgcgta ccgtctgaaa tcgaccgcca atcaacaggt
     4741 tcgtcagcat gtactcccca aaaaccgaag aaaaaatcat cagagaaact agggtcacac
     4801 cgatacggat gtttggaaac agcacctgga taataatcgt agacatcggg gcgcccaagg
     4861 tttctgctgc atccactaat tccttcagat taattaagcg catctggttg ttaagactaa
     4921 gatagacaat cggcaacgcc gtaataaaca atgcacctac caacactaca aacattggga
     4981 taccagtttc agcatacgtt cgaagcaaag ccgttaccaa aatcactcct ggtaaagcat
     5041 acggcagcag gagcagcttt tccagccaac gattcaaacg agggaaatac aaatgaatcc
     5101 aaataatggt gggaatcatc aacaacagaa tcgtcaccaa gaccacacct gctaataaaa
     5161 gtgagcgacc caccgcagca ataaattccc tgtccgtcag cagctgttgg aaccattgca
     5221 aggtaaaatc actaggtaaa agcgattttg accatttagt ggagctggca tacaaaatcg
     5281 ttccaataat cggcagcaag taatacagga caaagcagaa caaaatcggt tttattaact
     5341 ttttcacgtc tgcaccacct tgcctttctt ttgaataatc aacattgccg ttaccagcag
     5401 gacacctaaa gcgactgcca acgcactgcc taaattaggc ttggcgaaca catctcccga
     5461 cactaaagcc gcaattcgaa tcgttaaaag atttacatta ctgctggtca gggcatacgc
     5521 tgtttcatag gtccccatgc cattagcaaa taagatgaca aaggtgctgg caatcgatgg
     5581 tcgcaaaaaa ggcaaagcca ccttgaacca agcatacgct gatgaagcgc ccagcaattg
     5641 tgcggactcc agccattcct ttttcacttc cttcatgctg ggatacaaaa acaagacccc
     5701 taacggaatt tggaaatagg tatacgtgat ggtcaatccc caccaagaat acaaattgaa
     5761 gtccgctaga aatggcaaaa tcattttcag cacacctaca ttccccaaca aaataataaa
     5821 agagaatgcc agcggaatcc cagcaaaatt agctgccaaa ttggaaacaa aggtgatttt
     5881 ctcctgcaat ttagtcgata gattcattaa gcataaacat aaaataaacg tcccgattaa
     5941 cccaatcaca ctggagctga cagcaatcag tacgctgtta acaaaggcct gtgtgtaata
     6001 tttgttgctg aaaatatcac tgtaattggc caatgtccac tggttagtta aatcactgtg
     6061 caagctgcac agaaccataa aaagcagcgg cagcagtaaa aatgcacaga agataaagga
     6121 ggcaggaacc agaaccaagc gttttttcat ctctttcatc ccctcatcaa ttcttgaatg
     6181 tcctgcattt tctctgacgg tgcaattcct aataaccagc aaaacaatgg agccgccagc
     6241 agctgtggta cagtttgttc cttcagcttt tggcgtttat ttgaaatcaa ataaaaagga
     6301 acttcacgat gtgcagccaa gctgccgcca tgcagtccgt gcgcatccat cccgtgatcc
     6361 gcagtcacca cgacttgata acctttttgc agccattcag gaaggtaaat agccaataat
     6421 ctgtccgcct gattaaccgc tgctgcatat tcatgagaat cggcggtaaa tttgtgaccg
     6481 acatcatcaa tattcatcga atgtacgacc aaaaagtctg gctgatactg attcactaaa
     6541 taatgggcat ccgcaaataa atgcgaatca gggtaagtgt cctcactata aaagatgcca
     6601 tgctgaatca gtcgctcctc ctgatgctgg atgcgatcaa ccaaacgatt atagggggct
     6661 tgattgtaca actcgctgta ccagtggtaa gcggcggctc ctgtttttcc gccagcttcc
     6721 ttaactaatt caaacagcga ccactctttg gatcgctgag catggtaatt actatagatg
     6781 ccattttcat aggctggaac gccggtcatc agtacttcgt ataacggacg tgaattgctg
     6841 ggcagctcac tttttacctt caataagctt gcttgctggt gctcaactaa atgattcatg
     6901 aacccaagct gggcgacggc tgtgtcgtag cgacagccgt ctagtataac tacaatcagt
     6961 ttgtcaagtc tactttgcat aggtcagtac ctccgcctgc caaagctctg gaagctctac
     7021 agaggtcttg ttccaagctt cattgtcttc aactggcacc gccgcttcat aagcatcctc
     7081 cggcagtaat ttttcagcta cttctttagg cagctctacc ttgcgaatag gtcgcgcata
     7141 gccctttgcc aaattgattt gcccttcatc agataaaata tattcccgtg ctagctttgc
     7201 cgcattggga tgaggggcat ttttattgat taaggttgta tacccgctaa tgatggagcc
     7261 gtctgttggc acagttacct caaaacgctc ggagtcaatt tggtcacgat aatttagtcc
     7321 attgaagtcc caaacaactg ccacatcgat ttcacctttt tcaagatttg ctacgctggc
     7381 atcaacggtg gacaaacggc ctttttctgc catttttttg tagtaattca gacctggctg
     7441 aatatccttt tcgtcacctc cgttggcaat cgccgcagcc aatacagcaa attgtgcttg
     7501 gttggcaatc gtcacatccc ccacgctaac ttgatagtca ccctcttgca gctccttcca
     7561 gctagtcggt gcttgcttca cattctcttt atcggtgata aaggaaatgg ttcccgtata
     7621 gcttaacaac caatccccat catcatcctt tgcccaatcc ggaatttcct cccaatagtt
     7681 ggtcttataa ggtaaggtta actctttgct ttcagccaac ggaccgaagc tgataccgac
     7741 atcaccaata tcggcggttc cattttttcc ttctgattca aacttagcca attcttctgc
     7801 actggacata tccgtatcca cgtgacttaa gccgtattct ttttcaaggt cgttccaagt
     7861 gcctacccaa tttgcccaag tatccggcat tcccaagctg gcaatctctc cttcttcctt
     7921 ggctttttct ataatcgtat ctaaagagtc gtctgccgca gccgattgct tcgttgcttc
     7981 acctgagccg cagcctgtca gcaggatact tactgttgct gcacaaaata aaccgatagt
     8041 catgttacga attttttcat tcattttcat tcattcttct cccctttcga taattccatt
     8101 ctaacaaagc tttgtaatga gagaatcagt ttcttgaaaa ggtttcatga acaatgtaaa
     8161 ggttgtatat ggtttgtaaa tttcgacgaa ttcccgttaa ccttctaaag cttttgcggt
     8221 atgatagtag aaaaaaggag gctccctatg cagtattatg atcagcatct ccacacgtat
     8281 ttttcctttg attcagagga aaaatttgaa aactatttag cctatcagcc ggagtttttc
     8341 gtttctacgg atcactttga tttaaacaat ccatctaccg gaaaagatga cattccggat
     8401 tatccagcct atataaacaa actggactct ttaaaagaaa cctattccac caaatttctg
     8461 cgaggtatcg agattggcgt tgttccagga caagaagagc ggatcgtgga ttacctcgct
     8521 cagcatcctt atgatttaaa gctggtaagt attcatcaaa atggccgctt tgattatatg
     8581 gacgatgtgg ttttaaccaa agatcccatt caaactacca aggaatattt tgatcagatg
     8641 ttgcaggtct tgacggattt tccagaagga gatattttga ctcactttga ttatggatta
     8701 cgccgcttca ccttcacagc ggaagaattc gcggagcatt ttgaggctca gctgctaacg
     8761 attttcaaaa aggttgttga acgagaattg gcgatggagt taaatgccaa gagcttcttg
     8821 aaatacggca acgaagacct ttaccgttat gccgttcctt tgtatcaaag tgtcggcgga
     8881 aagctcttta ccttaggctc ggatgctcat gttgccgaag attatcagct gggctttaaa
     8941 aaaatgagtc agctgttggc agactgtcag gtagaaaagt tgctagtcat tcaaggaaaa
     9001 gaacgccgcc tgacgccgct gccgattttg tgattcctat ataatagaag aaagtggtga
     9061 aaaaaatgag caacctgtgg gactacaatc aagaggcacc catccattat ttaattgcgc
     9121 gacattggga tgcgttgaaa attgaggcgg tttgccgatc cttgttagca gccgttccaa
     9181 aacaacaact ggaaaatttt ttggtggctg acagcttgca gcgggaaaaa gttcaggctt
     9241 attttgcagc ctttaaagat cagcctttgg aatatttgca tgcgcagttt catctttttt
     9301 atcaagtagc cgccccagat gactacaacg atttacgagg acagctgcaa ttgacctttc
     9361 aagcggatga aaccgcctat accgttttat tgggaatggc tcgccttggt gatcaggcga
     9421 aggtcgagtg gcggattttc gatatttgat ggcaggatca aggcgtttca gttgcaaacc
     9481 ttcctctctt tcaattagaa aaaccgcagc aggaagaagt ttgtgtgctg gtcaccgatg
     9541 ctggtgaaat tcgtctccgc ttatttcctg accaagctcc cctggctgta gccaactggc
     9601 gctccctagc tgagcaaggc gtttatgaca acacaccttt cggctcgtgt cattaaggaa
     9661 tttgttattc aaggtggcgc tttggatggt tctggtgatg aagctgtttc cagctatgag
     9721 ggctttttcg ctgatgaagt gcatcgcgga ctttatcatt ttaatggcgc cttggcctta
     9781 ggggaccacg gcccccacac caacggcaac cagtttttta ttgtgcaaaa cacaaaggcg
     9841 caggctgacc tgctaatgta aagcaagcat acttggagct tggaggacta ccggagctgg
     9901 atggtcgtta tactgttttt ggacaggtat accaagggtt agaaatcgta gcagcgatcg
     9961 cagctcagcc gacaaacagt gaagatgtac cgattgagcc agtgttcatt cgacaaatta
    10021 acttcgaaaa aacatcgtaa aaagacactc actgacttca gcaaaagctc tattcttgag
    10081 ctggctgcag ctagtgagtg tctttcattt atccataaga taaagcttgg attcaataaa
    10141 taaataaatg ttttggttaa agctgtgacc agcaaatgag gaaccgtgcg agccagttga
    10201 tctgccaatg caaacatttc tttaggtgaa ggcgctggcg tgtctgccaa cggatactcc
    10261 agtcccaatg cttggtaacc gtgcatacca tagcagtggt acggtagtaa ttctaacggt
    10321 acgaaccgat caagatctgc cacaaaacaa gcaatcgtca gcagctcggt caggttcata
    10381 ttgatacctg gaaccacagg aactcgaatt cgcacttcgc agttaggata ctcaattaaa
    10441 aagcgacgaa tattcttcaa aatttgttgg ttatctaagc cggtccattg tttgtggagc
    10501 ttgctggtaa acatttttac atccacaaac aatgtatcca catagggagc taccttttgg
    10561 atttcattat aagcaccaca aaagctggtt tccagtacgg tattgattcc aatgtatttc
    10621 gatttttgca aaatttcttt agcaaaatcc gcctgaacca gtgcctcgcc tccgctaatg
    10681 gtcactccgc catccgagtg aaagtaaaaa acagcatcct tctcaatctc atccattacc
    10741 tcttcagcgg tcatggtctg gccaaatcct ttttcaatct gcttgctttg tgattcggga
    10801 atcgaacacc aggcacagcg taatgggcag ccctttaaat aaacaacggt ccgtaaacca
    10861 gcaccatcat gtagagaacc ctgttctatt cttaatactg ttccttccat atagattttt
    10921 gaattacaca ctagtttaaa ctaaaacaca caaggaaaaa cttaccattt aatcataaaa
    10981 ttagctcata taaatatact aggatgtaaa atagactggt caaaatctgg tcaaaaaaga
    11041 ggactaatac aggtagtcct cttttttgtt attttaatat tctaaatcat tttttattgg
    11101 tttattccct tattcgatgt agactcaaat cactatatca ctagatatca aacggcgtca
    11161 tacggaatca aaaacatcaa atttgacctg ttaaatggtc attgaataac attcaaaatc
    11221 aataaaacgg agtcagacac ggagtcaaaa acgctgagag taaaaaatat atacagcctt
    11281 gtaggtaaag taccacaggg ctgtatattt tttatttcag attaccttaa atgattccat
    11341 ttacttttta tctcattttt tcctactagt tagtatcaaa tactctccta ttttctcaat
    11401 aaactatttt tggatacatg gtatctgaaa cagctaaaac cttgtcatta cagcaaaaaa
    11461 tcacgttatc cagactgtag acaaagtcca ttttatggat gatgtctaca gtcttttctt
    11521 ttataataga tttatgaaaa cgaatgcaag gagtgatagc tatgatgtct aaaaacaata
    11581 cgaatggtag aaatcaattt gcgatgctta cgattgatga tctagtttct caagatcatc
    11641 tcgttcgaaa gattgatgcc gcgcttgatt ttgaatttat ttatccaatc gtcgaagcca
    11701 cttactccga tttaggtcgc ccaagtatcg atccagtgat tctgatcaaa ttggtgttta
    11761 ttcagtattt attcggtatc cgttccatgc gacaaaccat taaggaagtc gataccaatg
    11821 tcgcttaccg ctggttttta ggctactcct tcgaggagaa aatcccccat ttctcaactt
    11881 ttggaaagaa ttatgttcgc caatttagag aaacgacggt gtttgaggat atttttgcct
    11941 atattttaga acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt
    12001 ctacccacat caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg
    12061 aagcgaaagc ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg
    12121 gaaaacgccc ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa
    12181 gtaaagctga tccggaaagc ggctactacg tcaaaggcga acgagaaaaa caatttgctt
    12241 attctgccca tacttcatgc gacgacaatg gatttattct tagcacaatc atcactcctg
    12301 gaaatattca tgacagtcaa gtcgcctttc agctcgtcaa acaatcgaag agactttttc
    12361 ctgaaatcaa ttgtgttgtc gctgatgctg ggtataagac acctaagttt gttcactttt
    12421 tgactcattt gaaccttcga ccttgtttgc cttattcaag gcccaaaggg aaaaaggggc
    12481 tattatcaaa aaatgagttt ctctatgacg agtattttga ttgttatatt tgtccgcaag
    12541 atcaaatgct cgctttttca acggttactc gagaaaggta ccgagaatac aaatccaatc
    12601 caaaagaatg tgtgaactgt cctctgctaa accaatgtac caaaagtaaa aatcatcaac
    12661 gagtcatcac gagacatgtt tggggagatc tgatggatga agtggaacat cttcgcctaa
    12721 cagatctgaa taaatcaatt tacaaaaaac ggaaacaaac gattgaacga atatttgcgg
    12781 atgcaaaaga aaagcatggg atgcgttgga ccaagtatcg aggcttggaa aaagtcgcga
    12841 cgcatacgat gctagtgttt gctgcaatga atttaaagaa attagccacc tggctatgga
    12901 aaggcaagga gcctttattt ttttgttcaa aaattcgaaa cgaagtagat aaaaagctgt
    12961 tccaagctcg agttacgagt ttggaacagc ttttgtctac agtctgcgat gtcggagaca
    13021 tctcgttttc tattaggaat caactcaggt tcaaaagaag cctcccggtc tctgggaaca
    13081 tcaatgttta ctttaccaaa gctcgtttta agcgtcttat tgccataacc gtttcgtcga
    13141 ttgtcatgtt tctttggctc tttagaatgg gcgtcatacc ctaaatggtt attcaattct
    13201 ccttgaagca ttttttcaaa taagggacca aacacatctt ttaaaacatc ttgcatatcc
    13261 tctacagatt cgggttgata agtattcaaa atggattcag ctaacttctc agcatcagga
    13321 tttcctttct ttctagccat cgtgtaatca cctcttgatc ttattttaga aaaataaaaa
    13381 ccgtgaagca accacccgcc taggattgat ggttacttac acggtttata ttacactctc
    13441 atttttggtg ctaaaaagca ccgaaaattt taattattgt caattaataa ttcttttgga
    13501 ttccttctta atatattgaa tgaagaaata atcaatgata caagaagtac taaactcata
    13561 aatataacta cataaatcat gaatttaggt aatacattga tatctaaact tgataatgtc
    13621 ttgttaaatc cttcagcttc tgccccacca cctaattggc tagatgcaga ttgtttagct
    13681 atttgtttag ctatatcacc agtaacctta ttcaatatat tattcccaag cttgtcagct
    13741 gtatattgag ctaagaaata ggaaccaacg aatgcgggga ttgatataaa taccatctca
    13801 attaggaatt gaccaaaaat ttctaatttt gatataccta atgatagtaa tactgctatt
    13861 tctttctttc tagcattcat ccataagaat aataataatg aaactacaac tcctgcaaat
    13921 attaatgagc caaaaaataa tttatttgaa attgagtaaa tacctgatat agattgttgt
    13981 aatgcagggt aatttgatga acttttaatc aaattatatt ctctccaatt tatatctaat
    14041 ttcccaagat cttttattac actatcaaga ttcttatcac cttttacaaa gaatgttgca
    14101 tcttggtata cagctgtatc ttcggtatta ccataaactt tagcagctgt atgaatatca
    14161 gtgattaatg tattttcgta tagttcttgt gctgcactta ctacaccgct attatgccca
    14221 tcgaatagac ctttaatttc tacttctact gtttcatctg cacctttctc attatcagca
    14281 tcaaataaat tagattttat ttttatctta tctccaactt taaggttatt tttttcagct
    14341 aaatctttat gcattaagat tttattttta tcttcatttt ctaaatgctt tccttctact
    14401 aatttatatg cttctgatac aaattttgtt tcttttgttg agtcgttaac cccagttaac
    14461 ataactgttc ttttaaaatt ctttgctctt tcaggtgatt gattcgcaag agtctcttga
    14521 gtttcaataa tatcataatc aactaaatct gcaacactgt ttattctttt tacataggag
    14581 tctatactat ctgtttgaga tatcttttta atatcttcac cttttacatt tcccccacct
    14641 ctaggtgttc ctggatttac ttgtctattt atctccatag aaaaactatt tgttatatta
    14701 gcaaatgttt cttttgaagc tctgtccgta gcatctttaa tagataaact aatgagactt
    14761 aaagttgaca tagataaaat aactaatatg ataattaatg attttaaact ttttctagtt
    14821 acataagcaa atgcattttt tatcattatt caacctccaa attcatttta tttacttttt
    14881 tcaacttttt accacttagt tctaaaatga tatccgcaga atctgctact tccttactat
    14941 gtgttacaac tattacacat ttatttctat ctttagctaa tgtctttaat atattaatta
    15001 tttctccagc agtaacactg tctagattac cagtaggctc atcagctagt attattggag
    15061 catctgatac caatgctcta gcaatagcta ctctttgttg ctgtccacca gataatttca
    15121 taacatttct ttttatttgt tttttatcta aacctagttc aaacaagata ctttcatctg
    15181 ctgatttatt tactaatcta atattttcaa ttggtgataa ataatctatt aaatta
//
LOCUS       DAZKSU010000064        15209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000064 DAZKSU010000000
VERSION     DAZKSU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.064"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..252)
                     /locus_tag="ACG8Z3_002053"
     CDS             complement(22..252)
                     /locus_tag="ACG8Z3_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824354.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="ACG8Z3_002054"
     CDS             complement(688..1254)
                     /locus_tag="ACG8Z3_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0824355.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="ACG8Z3_002055"
     CDS             complement(1254..2210)
                     /locus_tag="ACG8Z3_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0824356.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="ACG8Z3_002056"
     CDS             complement(2210..2866)
                     /locus_tag="ACG8Z3_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0824357.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="ACG8Z3_002057"
     CDS             3236..5254
                     /locus_tag="ACG8Z3_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0824358.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5324..5530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5681..8095
                     /gene="leuS"
                     /locus_tag="ACG8Z3_002058"
     CDS             5681..8095
                     /gene="leuS"
                     /locus_tag="ACG8Z3_002058"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0824359.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="ACG8Z3_002059"
     CDS             8443..9996
                     /locus_tag="ACG8Z3_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0824360.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="ACG8Z3_002060"
     CDS             complement(10377..12161)
                     /locus_tag="ACG8Z3_002060"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824361.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="ACG8Z3_002061"
     CDS             complement(12176..13897)
                     /locus_tag="ACG8Z3_002061"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824362.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="ACG8Z3_002062"
     CDS             13997..14758
                     /locus_tag="ACG8Z3_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0824363.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4949 a         2785 c         2990 g         4485 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcct
//
LOCUS       DAZKSU010000065        14650 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000065 DAZKSU010000000
VERSION     DAZKSU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.065"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="ACG8Z3_002063"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="ACG8Z3_002063"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0824364.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG8Z3_002064"
     CDS             952..1257
                     /gene="rpoZ"
                     /locus_tag="ACG8Z3_002064"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0824365.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="ACG8Z3_002065"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="ACG8Z3_002065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0824366.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="ACG8Z3_002066"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="ACG8Z3_002066"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0824367.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /gene="fmt"
                     /locus_tag="ACG8Z3_002067"
     CDS             4417..5355
                     /gene="fmt"
                     /locus_tag="ACG8Z3_002067"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0824368.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG8Z3_002068"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="ACG8Z3_002068"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0824369.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="ACG8Z3_002069"
     CDS             6726..7466
                     /locus_tag="ACG8Z3_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0824370.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="ACG8Z3_002070"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="ACG8Z3_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0824371.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG8Z3_002071"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="ACG8Z3_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0824372.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /gene="rpe"
                     /locus_tag="ACG8Z3_002072"
     CDS             10609..11259
                     /gene="rpe"
                     /locus_tag="ACG8Z3_002072"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0824373.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="ACG8Z3_002073"
     CDS             11260..11898
                     /locus_tag="ACG8Z3_002073"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0824374.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="ACG8Z3_002074"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="ACG8Z3_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0824375.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="ACG8Z3_002075"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="ACG8Z3_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0824376.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..>14650
                     /locus_tag="ACG8Z3_002076"
     CDS             13675..>14650
                     /locus_tag="ACG8Z3_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0824377.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         4923 a         2719 c         3186 g         3822 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag
//
LOCUS       DAZKSU010000066        14466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000066 DAZKSU010000000
VERSION     DAZKSU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.066"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..203)
                     /locus_tag="ACG8Z3_002077"
     CDS             complement(78..203)
                     /locus_tag="ACG8Z3_002077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824378.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLTELGRSRPSSIGG"
     gene            complement(449..2446)
                     /gene="tkt"
                     /locus_tag="ACG8Z3_002078"
     CDS             complement(449..2446)
                     /gene="tkt"
                     /locus_tag="ACG8Z3_002078"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0824379.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2548..2799)
                     /locus_tag="ACG8Z3_002079"
     CDS             complement(2548..2799)
                     /locus_tag="ACG8Z3_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0824380.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2946..3569
                     /gene="lexA"
                     /locus_tag="ACG8Z3_002080"
     CDS             2946..3569
                     /gene="lexA"
                     /locus_tag="ACG8Z3_002080"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0824381.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3748..3966
                     /locus_tag="ACG8Z3_002081"
     CDS             3748..3966
                     /locus_tag="ACG8Z3_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824382.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4254..4766)
                     /locus_tag="ACG8Z3_002082"
     CDS             complement(4254..4766)
                     /locus_tag="ACG8Z3_002082"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0824383.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4780..7077)
                     /gene="recJ"
                     /locus_tag="ACG8Z3_002083"
     CDS             complement(4780..7077)
                     /gene="recJ"
                     /locus_tag="ACG8Z3_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0824384.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7219..7662)
                     /locus_tag="ACG8Z3_002084"
     CDS             complement(7219..7662)
                     /locus_tag="ACG8Z3_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0824385.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7663..8451)
                     /locus_tag="ACG8Z3_002085"
     CDS             complement(7663..8451)
                     /locus_tag="ACG8Z3_002085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0824386.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8448..9389)
                     /gene="rnz"
                     /locus_tag="ACG8Z3_002086"
     CDS             complement(8448..9389)
                     /gene="rnz"
                     /locus_tag="ACG8Z3_002086"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0824387.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9566..10153)
                     /locus_tag="ACG8Z3_002087"
     CDS             complement(9566..10153)
                     /locus_tag="ACG8Z3_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0824388.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10374..11687)
                     /gene="obgE"
                     /locus_tag="ACG8Z3_002088"
     CDS             complement(10374..11687)
                     /gene="obgE"
                     /locus_tag="ACG8Z3_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0824389.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11975..12985
                     /gene="gap"
                     /locus_tag="ACG8Z3_002089"
     CDS             11975..12985
                     /gene="gap"
                     /locus_tag="ACG8Z3_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0824390.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(13070..14005)
                     /locus_tag="ACG8Z3_002090"
     CDS             complement(13070..14005)
                     /locus_tag="ACG8Z3_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0824391.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14191..>14466
                     /locus_tag="ACG8Z3_002091"
     CDS             14191..>14466
                     /locus_tag="ACG8Z3_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824392.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT         4043 a         3224 c         2546 g         4653 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaggagcaa tcgctttttt attgatcacc atttttcttt taggtctatg gtgtcgggca
      181 ctgcgggtct tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa
      241 gttctcttct taaaacaaac ttaattaatt aaattatact tttaattctc aagaaacaca
      301 aactaatccg aagaattctt tgttttgtca tttaaatgta acatagccga aaacgaaaag
      361 taatattgat ttattcttct aacacataaa gaacagctgt ctccaacttg cagacagctg
      421 ttcttatcag tcaaacaatt gcttggatct atgatagttg attgtacgta ttcacgacat
      481 tttctactgt aaatccaaat tcttctaaaa ttttagtgcc aggggcagat gcaccgaaat
      541 gatcgattgt aatcgttgcg ccctctgttc ctacgtagcg ttcccaacca aatgatgcag
      601 cagcttcgat agccacacgt ttcttcactg atttaggaag tacactttct ttatattcag
      661 aagattgttt ttcaaagagg tcaaagcttg gcatagaaac aacagaaaca tctttgcctt
      721 tttcagccaa ttctttttga gctttcacag ccaaatcaac ttcagaacct gttgcaatca
      781 aaataccttc tggtgtttct ccttgtgatg gagaaagaac atacgcgcct tttttcacca
      841 tttcatctgc tacttctttt gtagaaggca atactggaag gttttgacga ctcaatacaa
      901 ggatagtagg cgcatctgtt gtctccatcg ctactttcca agcagcacgt gtttcatttc
      961 cgtctgccgg acgaatcact tgaacaccag gcatgcagcg aacagatgct agttgttcaa
     1021 tcggttcgtg cgttggtccg tcttctccga cagcaaccga gtcatgtgtt aagacgtaaa
     1081 caacaggagt atgttggatt gctgctaaac gaactgctgg tcttagataa tcgacaaaga
     1141 caaagaatgt tccaccgtag atacgagttc cgccatgaag ttggatacca ttcatagccg
     1201 aagccattgc aaattcgcgg acaccgaacc agatgttgcg tccttcataa tgttcaggag
     1261 tgaaatcttt atctgcagcc accattgtat tattggaacc tgacaagtcc gctgaaccac
     1321 cccagaagct tggtatagct ttcgataatt cttggatgac ttctttactt gaaacacggc
     1381 ttgcttgact gcttccaact tcataagttg gcaattctgc atcccagttt tctggtagtt
     1441 tgccatcaaa agcatcttca aattgttgtg ctaattcagg ataagctttt ttataattgg
     1501 caaacatttc attccatgca tcttcagctt tttgtccttc ttctatcatc gtttgatgga
     1561 agcgggcagc tacttcttct ggaacagtga aatctggata ctcccaacca taaacttctt
     1621 tagccatctt gatgccatct tcaccaatag gagcaccatg aacagcagat gttccttctt
     1681 taggagcacc atagccgatg actgttttta cttcgatcaa tgttggtttg tctgtttcag
     1741 cttttgcttc ttcaatcgct ttagagattg cttctaggtc atttccgtct ttcactaaga
     1801 tatgttgcca gccatatgct tcatagcgag caccaacgtt ttctgtgaaa gcttttgaag
     1861 ttggaccgtc tagtgaaata tcgtttgaat cgtataaaac gatcaatttc cctagtttca
     1921 tatgacctgc catcgaactt gcttcttgag ataccccttc catcaagtct ccgtcaccgc
     1981 atattgcata cgtatagtga tccatcacat taaagttttc tttgttatac gttgctgcaa
     2041 ggtgcgcttc tgccattgcc ataccgacag ccattgcaat cccttgtcct aatggccctg
     2101 tagtggcttc tacaccatct gtgtgatgta cttcaggatg tcctggtgtt ttagaatccc
     2161 attgacggaa ttgtttcaaa tcatcgattg tcacttgata gccagctaaa tgaagcaagc
     2221 tatacaacat tgctgaccca tgtcctgccg aaagaacgaa acggtcgcgg tcagcccagt
     2281 tttttgatgt cgttggattt actttcaggt gttttgtcca taacgcatat gccataggtg
     2341 ctgcgcccat cggcaatcca ggatgtcctg agtttgcttt ttggactgct tcaatactta
     2401 atgtacgaat tgtgttaacg ccaagttggt caattttgtc aaacaaaata acccctccta
     2461 ttaatttagg ttttttttac ctttatttct tacactttca ttctataaga tgtcacacga
     2521 aaaagcaatc tttttcagta ttttttacta aaagttgtca tttcggttgt gtaagccttt
     2581 tttcttttgg atttctttta atttttccgg ggtcacgtca ttgccctcag gatcgacgac
     2641 tttcattcct tcgatatgat gacgcatacc ggaacgaaaa gcttccaaat actctttgcg
     2701 taagttgatt tgttcttgtt cttcggcttt tgagagacca ttttccttct ttttacgtgc
     2761 aagttcattg atcctctcta ttttttcagg tgacagcatg ctcagccctc cttttactct
     2821 ccttcatatt atataaaaac tgaaaaaaaa acaactagat aaactaaata aaatacgaac
     2881 attcgtttga tttagcgctt tttatatggt actatttatt tatcaagtga aagaaggtgt
     2941 accttgtggc ccgacaaact gaatcaagac aaatcgaagt cttacgatat atccatgaac
     3001 aagtaaccca aaaaggatac ccgccgactg tccgtgaaat cggggaagct gttcagcttt
     3061 cttctacttc tactgttcac ggtcatcttg cacggcttga aaaaaaaggg tttatccaaa
     3121 gagatccgac gaaacctcgt gcaatcgagt tgacaaaaca aggtttagaa aagattggga
     3181 ttcgtccaac aactattcca gtacttggcg tcgtaacagc tggagaaccc attttagcag
     3241 tagaggatgc ttcagacttc tttccaatcc ctcctaatct ttcttccgaa gaaggcagtc
     3301 tctttatgct gacaattcgt ggagatagta tgatcaatgc tgggatcttg gatggtgaca
     3361 acgtgatcgt tcggaagcaa gaaactgctc aaaatggcga tattgtcatt gcaatgactg
     3421 ccgaggacga agcgacttgc aaacgattct ataaagaaaa aaactttttc cgtctgcagc
     3481 ctgaaaacga cttgctcgaa ccaatcatct tagatcaagt atctatatta ggacgagtag
     3541 taggacttta tcgtagtcat atctattaat tgctactaat aaaagaaaac tttttaaaaa
     3601 gagtttgaga cgatggtctc aaactctttt ttcgcccggg ttcttcttct ttcaaatttt
     3661 tatcacttcc ctctttacaa accgaacaaa tattcgtata ctaaatatac gaacaattgt
     3721 tcgcataaat taacgaggtg agaattcatg aaatcaatga gacgtcttgg cttagtatta
     3781 actgttttgt ttttaggtat tattattggt aatttcggca tacgtccagc tttacttgtg
     3841 tttgttccgt tatttacttt gtggttcatg ttgtgggatg aaaagaagta ccgtcaatct
     3901 gagcggcgca gaattcatga agatcatttc tatcatgagc cctactacag atccaggcaa
     3961 tcataataag gattgactgc taattattca aaagacaaga agttaaactt ttacgggggt
     4021 gtgacaaaaa tcggtcacat ccttttttcg aataaacggt gttctaaagc cagtttttcg
     4081 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
     4141 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
     4201 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
     4261 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
     4321 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
     4381 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
     4441 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
     4501 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
     4561 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
     4621 attcgttttt cttttgcata atttacgatt tgcttcgtag cttcccgata agcgtcacca
     4681 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     4741 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     4801 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgccg
     4861 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     4921 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     4981 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     5041 ttcttgagaa tggatcagtt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     5101 caaataggcc tcatcaggcg aaatccccat cagataaaca cgattgaatg aataagaccg
     5161 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataataacta gagagttcgg
     5221 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     5281 ctggataatc tcaggcacga atttcaacgt agaagaatca aatgctaaat aaaggctacc
     5341 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     5401 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     5461 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     5521 accaaaagcg accgcatcaa gctgactcga tgcattggaa aggacaaatt tcagatgctg
     5581 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     5641 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     5701 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     5761 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     5821 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     5881 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     5941 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     6001 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     6061 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     6121 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     6181 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     6241 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     6301 cgcctgaagt cctatccgtt ctgaatgatg aatggcattg attcctaatg aaacaaggat
     6361 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     6421 aaattctaca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     6481 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     6541 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     6601 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     6661 gattccttct tcgatctttt cttggtatac agattggttt ggtccatacc catgctcaaa
     6721 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     6781 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     6841 gattacagct tcttggattc ggctgattgc tttatccata tcgtgcatca gatacggatc
     6901 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     6961 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     7021 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     7081 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     7141 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     7201 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     7261 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     7321 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     7381 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     7441 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     7501 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     7561 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     7621 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     7681 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     7741 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     7801 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     7861 atcagcctta tcgaagaaat ctgtctcgat tggacctgga ttcactgtcg taacagctac
     7921 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     7981 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagacg cgatattaat
     8041 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     8101 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     8161 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     8221 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     8281 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     8341 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     8401 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     8461 tcctcgcgaa atggaatctc aatgctatca aaatctttca tgatcgtcgt gttttcaaac
     8521 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     8581 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     8641 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     8701 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     8761 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     8821 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     8881 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     8941 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     9001 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     9061 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     9121 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     9181 aaaatatgat cgccgtgtaa atgtgtaata aaaattttac cgatttttct cggacgaata
     9241 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     9301 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     9361 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     9421 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     9481 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     9541 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     9601 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     9661 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcgg gtaatttctc
     9721 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     9781 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     9841 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     9901 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     9961 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
    10021 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
    10081 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
    10141 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
    10201 tgtaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
    10261 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
    10321 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
    10381 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
    10441 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
    10501 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
    10561 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
    10621 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
    10681 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
    10741 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
    10801 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
    10861 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
    10921 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
    10981 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
    11041 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggg
    11101 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
    11161 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
    11221 tctagttcga tttttcgctc ttgtcctggt tcgccgtttt cagcaatttc cggtgcagga
    11281 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
    11341 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
    11401 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
    11461 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
    11521 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
    11581 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
    11641 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
    11701 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
    11761 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
    11821 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
    11881 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
    11941 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
    12001 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
    12061 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
    12121 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
    12181 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
    12241 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
    12301 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
    12361 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
    12421 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
    12481 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
    12541 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
    12601 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
    12661 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
    12721 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
    12781 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
    12841 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
    12901 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
    12961 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
    13021 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
    13081 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
    13141 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
    13201 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
    13261 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
    13321 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
    13381 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
    13441 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
    13501 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
    13561 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
    13621 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    13681 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    13741 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    13801 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    13861 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    13921 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    13981 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    14041 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aacccgcgaa
    14101 ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat tgtatttccg
    14161 acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac catagacgaa
    14221 ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat cgctaaccga
    14281 atgggacgtg ctatacaaac tatttataat gtagtcaata agttcaagca aggcaagact
    14341 gctcttgatt attggcacca gtataaagaa aataagaaaa aatgtggtag aaaagtcatt
    14401 caattacctg ctcatgaagt agattacatt aaagagaaag tcactcttgg ttggacgcct
    14461 gacgtc
//
LOCUS       DAZKSU010000067        14075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000067 DAZKSU010000000
VERSION     DAZKSU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.068"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..587)
                     /locus_tag="ACG8Z3_002092"
     CDS             complement(<1..587)
                     /locus_tag="ACG8Z3_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824393.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVF"
     gene            complement(735..1277)
                     /locus_tag="ACG8Z3_002093"
     CDS             complement(735..1277)
                     /locus_tag="ACG8Z3_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0824394.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            1412..1801
                     /locus_tag="ACG8Z3_002094"
     CDS             1412..1801
                     /locus_tag="ACG8Z3_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824395.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1805..2569
                     /locus_tag="ACG8Z3_002095"
     CDS             1805..2569
                     /locus_tag="ACG8Z3_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0824396.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2698..3645
                     /locus_tag="ACG8Z3_002096"
     CDS             2698..3645
                     /locus_tag="ACG8Z3_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0824397.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3775..4515
                     /locus_tag="ACG8Z3_002097"
     CDS             3775..4515
                     /locus_tag="ACG8Z3_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824398.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4806..5474)
                     /locus_tag="ACG8Z3_002098"
     CDS             complement(4806..5474)
                     /locus_tag="ACG8Z3_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0824399.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            5608..5895
                     /locus_tag="ACG8Z3_002099"
     CDS             5608..5895
                     /locus_tag="ACG8Z3_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824400.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            6311..7849
                     /locus_tag="ACG8Z3_002100"
     CDS             6311..7849
                     /locus_tag="ACG8Z3_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0824401.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7846..8811
                     /locus_tag="ACG8Z3_002101"
     CDS             7846..8811
                     /locus_tag="ACG8Z3_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824402.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8798..10120
                     /locus_tag="ACG8Z3_002102"
     CDS             8798..10120
                     /locus_tag="ACG8Z3_002102"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0824403.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      10183..10287
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10361..11104
                     /locus_tag="ACG8Z3_002103"
     CDS             10361..11104
                     /locus_tag="ACG8Z3_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824404.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            11119..12111
                     /locus_tag="ACG8Z3_002104"
     CDS             11119..12111
                     /locus_tag="ACG8Z3_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0824405.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            12123..12860
                     /locus_tag="ACG8Z3_002105"
     CDS             12123..12860
                     /locus_tag="ACG8Z3_002105"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824406.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12850..13572
                     /locus_tag="ACG8Z3_002106"
     CDS             12850..13572
                     /locus_tag="ACG8Z3_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0824407.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(13656..>14075)
                     /locus_tag="ACG8Z3_002107"
     CDS             complement(13656..>14075)
                     /locus_tag="ACG8Z3_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0824408.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4713 a         2387 c         2912 g         4063 t
ORIGIN      
        1 ataaatacac ctagactgac gatgaagagc atatccaaca gatttcctgc gacttggatc
       61 gtcgaagggt tggtcagtgg cattaaagca aggacaaaaa aggaaaacaa gctgtaaata
      121 aaacaggtga caggaactaa tcgaaagaag agcggacatt tcaattggct aaaaatgagt
      181 gaaaaaataa aataagtaaa aatggataaa atcacgacta aaccattcat gataaagaag
      241 aaaagcgtat tgtgattaaa cgaataaaaa agaattgcta aaaaagaaaa cgttttaaat
      301 gcaatcaata cgcttgaagc ttcgtttaac gtaccaggat gtcgatgaaa aaaataggca
      361 ccgaaataaa cagaaaacag taagccgcaa agagaagaca aaatcaaaaa aaacggattt
      421 tcttcggctt ctgtgatatc agaatgatcc aaaagcaacc agcctaatgc actagaaggc
      481 gcatgtgagg tcttaaggta tgaataacat agaaaaaata agatcgtcaa agttgcaaaa
      541 aaagagccta aatgtaaaaa attagtggtt ctacgttgaa tttccataat atctccaaat
      601 taaattgata agtatataaa gtataataac aaattgatga gtatatgtcg atgtttttta
      661 atcaaaataa taaataaaaa ctgtctaaca tttaaaattt aatttagata ttagacagtt
      721 tttaagtttg tgagttattt tccttgtcgt tttctcactt ttttagtcca gaaaccacct
      781 gaaatatatt ttggctcata agaaatgata aaagcacgct cttcgatttc tttgatcagg
      841 cgatacagtt cacgttcgct ttttctagga gaaagtattt cgagaactag ccgctcacct
      901 tccagcccca ttgcagaagt ctgtgtcaca ccataaccgt gttctctcag tatacccggc
      961 aaacttttat cttctgcgct atttgctggc aaaatcgttg tgaccatgat gtatcctaat
     1021 gccaatttgt cttcaatcaa aattccgaca ctgataccta ctgcgtaacc taacgcatag
     1081 acaaataaat tcagtggatt atctaaacga tctaaaacaa gggataagcc gacgatataa
     1141 atcgtgatct cgaccatact gacaagtgga gcaatcagtc gatagccttt cattgtcaac
     1201 atgaaacgta acgtgttcag agtgatataa gcaaaattaa tgaggaagat catccccaac
     1261 atttttggat caatcatttg taaaactcct ttcatttata taacaacaat atcaaaaaaa
     1321 atcgtaagtg aagaaaaatt gaaactttat acatacaaaa aaatgataaa atgattataa
     1381 tagaattata aagataggtt aggtgattaa aatgaaaacg gtcaatcgtg tgttttgtgc
     1441 cagccacgtg ctaagtctcc ttctcagtat atacttaggt gtctattcga ttcaacagcc
     1501 aattgaggta gagaacattg tttttcagat gtcgctgaca gatggtttaa ttttaagcgt
     1561 ttttttcttt ttgatactgt ttattggtaa tattttcggt agcgttgcca gttttttgaa
     1621 tttttctatt tatccactcc tttcgctagc attaggagtg gtgggacttt gttctctgtg
     1681 cttatttccc gagccattca gtcctgcatt tatattattt ggtacactga acctgttcca
     1741 gacaactgtt ggagcatggt tgctatggcg atcaggaaat ttaatgaaaa taggtgagta
     1801 acaattgaat ccaattatat tgaaacgttc tgtccatcat attccagtgt tagaagttgt
     1861 tctgaaagaa ttaagagagg aacgtattcc cgttgtcatc tattatcacg gatggcagac
     1921 aagtaaggag ttagttttga cgcaaggaag aaaaattgcg cagcagggca ttcgtgtgat
     1981 cctgccagat gcgatgaatc atggggagcg caaacagccg gtttcttcta ttccatccta
     2041 tacattttgg gatagtatct acggaaatct gtttgaattt gacacactta tcaagcattt
     2101 agaaaaaaga ggtcttctac atgatcagct tgcagttggc ggggtttcga tggggggaat
     2161 gacaacatgc gccttgttgg ctaaacatcc tcatatagta gcgggcgtgt gtttgatggg
     2221 ttcccctgca cctttggaat atggaaaaga aatcgtaagg cgcgcatcgg aatataatta
     2281 tgtcgtacca aaagactatt ttgatttgat ttcatgggtt aagagctatg atttgtcatt
     2341 gcagccggaa aagttagctg gacgtcctct gttcttttgg catggacaag ctgacgaaaa
     2401 aatcccttat atacaaacgg aacgatttgt agaaaaaaat cggcataagt cttacggaaa
     2461 agaaattgtt tttcgttcct caaaaacaca gcgacatatg gtacaaatac cactaatgga
     2521 agaagcttcc acttttttag caaaaaattt gataaaaaag tataaataat tgaaacggaa
     2581 aatcactcat gacgatagag gattttccgt tttttatttt gggctataat aaactctgtt
     2641 aggaaaattt ggtataattg atagtaagaa tgacaacatg ttagaaagga agagagcatg
     2701 cccactttag cagatgttgc aaaacgtgcc aatgtttcaa aaatgacggt ctctcgagtc
     2761 atcaatcatc cggaacaggt gactgacgaa ttgaaagagt tagttttctc agcaatggct
     2821 gaactggatt accgtccaaa tattgctgca aaagcacttg tcacgaatcg gtcgcagatc
     2881 atcaaattat ttatactgga agaaatcgac acaactgagc cctattacat gaatttgctg
     2941 atgggaatcg ctaaacggat cggtaaagcg cattattctc tccaattagt tacaaatgat
     3001 gccttcgacg tcggttcttg tgatggatac atcatcacag gtgtcagaaa aaaagacttc
     3061 gattggatcg accgtttgga aaaaccagtt gtcttgtttg gggaaaattc tgaaggatat
     3121 gattatgtag acagcgataa tgaatatggt acagcaaaag caacgcaata tgccttagaa
     3181 agagattatc aaacgattat ttatatcggt atcgaagtgg acgagccttt tgaaatttca
     3241 agggaaactg gttatttatc tgtgatgaag gaagttgatc ttcctagtaa aatcttgcgg
     3301 tttccaaatc attcgacaga agtagaaaga tatatgacag aaaactgggc agattttaca
     3361 gataaaactt gttttgtatg tagttcagat cggctagcac taggagcagt aagagggatt
     3421 ataaatgctg gaggacactt gcccgaagaa tttggtgtga ttggatttga tggggtattt
     3481 ttagaccaaa tcagttcacc taaattgaca acgatcaagc aagatattat acgtatgggt
     3541 gaagtatgtg gagaaatgtt gttaaaaaaa atagaagaaa aaggggcgag tcaaggttat
     3601 cgtcgttttt ttcctgaact agttatcaga gagacaacga attaagtctt attactatat
     3661 atatatttat gaaattcatc gctattttct taaaataatc agcgcattgc tactgaaaac
     3721 aatgaatctg tgctattatt gagaaaagtt ccatttttga gaaggatggt ttgaatgaaa
     3781 gaaatcagac gaaaacattt ccgttttcct gcgtacgatg atgagttagg cgtaaaatta
     3841 accaacgaca agaagcgagt attatttgac ggacaagaag atcttttgac tgaacagaat
     3901 cagtccccgt ttgaacagat gaaagaattt catttcacga acacgaacac cgagaaaatc
     3961 cgtcgcaagc gtcgtgcaac aaatggattg aagtcagctc cgcgtaagga aaatttggaa
     4021 aagcataaag gaaaattacc ggattatacc ggccattacc aatcagaaat cacatcgaca
     4081 ggacgaaaaa aactttttgg tgattcctct cgttctgtca gacaaagtga aacaaggaca
     4141 gagtccaagc atactacacg aaacgcatct gttgacacga aggtgcggga gcgttcttat
     4201 tttgttccta aatatgtacc cgcatcgatt atcccagacc ctagagaaag caaaatttct
     4261 gaggatcatt taatcgaatc gatgaaaaaa acggatgact cttatctgtt gttcgataca
     4321 gaaccgacgc cgtatcaaga aaaaagaaat ggtgagccgt ctgtacgaac attcaatcga
     4381 acaaaaacgt tagaggaaac aaagcagtca gaagaaaaaa aatcgaaaaa aagccatggt
     4441 atcttagaac gctcattaca aggattgatc gatgatcaag gaaactcttt gggagaaaat
     4501 agctatttta aataagtgat atacaaattt atattcggct acagttggaa aggaaacgct
     4561 gcggcgtttc cttattttat actctatccg aaagtttata tcgtatcctc aaattttttt
     4621 gagtttaagc gaattttttc tcccaataag taagaaaata atggcaaaat aaaaaaacaa
     4681 actattttcg cagatcgttt gcaaaaatag tttatttttt tataaagaag attttactga
     4741 ataatatcat tttagtttac ataatgatat ttctgtaaat taaataaagt agtgaaaaat
     4801 aagaatcagt gctctaactg ttcaagagga tcaagtggtt caaatgtttc tgtagtctgt
     4861 tttgcttcca attggggcaa aacagatcca atttccagat acttcacttt attcaatgta
     4921 taagatagaa tagaacagct aagtaatgta tacagaaaat gtgtgaggtc caaataaagc
     4981 aaggagacaa ctaaaatacc aaaagtaatc aagaaaagac ttttgccaat cttaatatga
     5041 aaatattttt caatggcttg ataaatgata tctgttccgc ctgtgcttcc gccgaatcga
     5101 aagacgagac cgcaaccaat gccagaaaga atacctgtca tcaaactatt gatcagtaga
     5161 ctatctacag caaaatgtat atgaaggctc tcccataaat gtaaaaagaa aactagcgca
     5221 aatgttccga cgatcgtccg gagaaaagcg tgttttccaa aaagaaacaa tgagataagc
     5281 aataaaggta aattaaaaag cagtgaagtg acagaaggag cgaatccaaa aagattatgc
     5341 aaaagcaaag caataccagt aaccccacca tctgccagct tagatggaat attgattcct
     5401 gtcacagcta atccgtagat tcctgcgcct aaaatgattc cgcacaattc tgtaaataaa
     5461 tttaattttt tcatttctag cctcttttct tttgaaaatt ttcagcaact tctttattat
     5521 actgacttat actcttactt caagaggaaa atgaaaaaaa attatttttg gctgtttctt
     5581 tttatttatc aaataaggag tggaaaaatg cgagcagcag acttattgac aattcttaca
     5641 aaatcaccca aaaaaacaga gtatcaagga gtttttttgg ctcaagaaca aaaactggtg
     5701 cctgtgacaa tggtccagat aaataaagca aatcaattta tctttttatt tgaacacggc
     5761 aaagcaccac ttgctatgaa agacatcttt ctttttttga tgaagaaccg tgagaaagct
     5821 gtttattatc agtcaaaaga agaactcatt cctctttatg gagtgaaaga gatagaaaat
     5881 aaagtggtga tataagtaaa aataagaaaa caagacgttt atgatgtcaa aacaaccatt
     5941 ttttacattt ttggcaaaaa agtgtcatga tacagtataa tattcgttga acaggccaat
     6001 aaaggtgttc tcccccacga gatttctact ttattggctg tttcttttta ttaaaataaa
     6061 agttgtgtgt gtaaaaaaga tatagctgat tccaagtgac aatttcgtat agatttttat
     6121 ggtattcgta gtgaaaaaac gagaaccaaa aagttgatcc gactttgttg cttggattct
     6181 ttttttgtca gcatatttag aggtattttt ttcttttttt tgttcgtgct gtaaggttaa
     6241 ggcaagacaa gttagttggg aaaaaggaaa gaaatgttta tacttaaaga ataacagaaa
     6301 ggaaggtcgc atgaataatt ttatacaaac attaagtgaa agaaaaggcg agttgctgga
     6361 agctgcattt caacatctgt ccatttctct catttctttg ctgattgcag cactgattgc
     6421 tattccttta gcgatttggg cagccaatca taaaaaaatg gcagaagtct tattgcagat
     6481 cacgggtgtg ctccaaacga ttccttcact tgccttatta ggtttactta tcccatttgt
     6541 agggatcgga actgttccag cattgattgc acttgttatt tacgctttgc ttccgatttt
     6601 ccaaaacacg tatattggtc tagctgaaat tgacccgtct attgaagaag cagcggtagc
     6661 atttggtatg tccagaatga gacgcttgct gaaagtagag cttcccatcg cattacctgt
     6721 cattatctca ggtatccgaa ctgccttagt tttaatcatc ggtacggcta ctttggcagc
     6781 attgatcgga gcaggtggat tagggacatt tattttgtta ggtattgacc gaaatacacc
     6841 tgttttgaca ttgattggtg cgatcagttc tgccttgctt gcaattatat tcagcggact
     6901 gattcgctgg ttgcagcata agaaacctcg atattccatc attactttag cagcagtggt
     6961 gtgtttattt ggcggcgttt ctttatatca gtctgccttg tttaaaaatg aaaccatcac
     7021 gattgctgga aaattaggag cggaacctga aatcctgatc caaatgtata aagaattgat
     7081 tgaagacaac acaaaaacag aagttgaatt aaaaccgaat tttggcaaga cgagtttttt
     7141 gttcaatgca ctggatcaag gtcaaatcga tatctatcct gagttcacag gtactgtcct
     7201 agaaagcttg gtgaaggtac ctgaagcaat gaaaagtgct gatttgacaa aagaaaaaac
     7261 atatgacgaa gcagaagaat tattgaataa gcaattcgat atgacattat taaaaccaat
     7321 ggcttaccaa aatacgtatg ccttggcagt caagagatct tttgcgaacg aacatcaact
     7381 taaaaagatt tccgatttgg cagctatctc tgatcagatc aaagcgggat tcacactgga
     7441 atttattgat cgttcagatg gatataaagg aatccaagaa gcatatggct tgaatttttc
     7501 aagtgtacaa agcatggagc caagtttgcg ttaccaagca atcaataatg gagatgtcaa
     7561 tattgtggat gcctattcca cagatagcca gctcaaggag tacgatctag tgactctaga
     7621 agatgatcga gggttattcc cagcttatca aggcgcaccg ctgatgaaaa aagattttgc
     7681 taaagaacat cctgaaatcg ttaaagcact gaataaatta agcggaaaaa taacagagga
     7741 tcaaatgatt gatatgaatt accaagtcaa tgtcgaaaag aaaaagccga cagaagtagc
     7801 acgaatcttc ctgaaaaatg aaggattagt aaaggaggaa gatcagtgac taaattaatc
     7861 gagttcaaac atgtgcaaaa aaagtatgat ggaaagtatg tcatcgatga cctaaatctg
     7921 gcaatcaaaa aaggagaaat ctttgttttg gttggtcctt ccggtagtgg aaaaacaact
     7981 actttgaaaa tgatcaatgg attatctaaa ccatcagcag gagacatcta cttcaaagga
     8041 aaatcgttaa atgaatataa tctgcagaaa atgcggtgga atatgggata tgtgctgcag
     8101 caaatcgctt tgttcccgac catgacagtc aagcaaaata ttgaagtcat ccctgaaatg
     8161 ttaggttggg aaaagcagaa aagagcagac cgtgtagatg aacttcttca aaaagttggc
     8221 ttatctcctg atatttatcg agatcgtatg cctagagagt tgtctggagg agaacagcaa
     8281 cggatcggga tcatccgagc tattgctgca agcccagatg tgattttgat ggacgagcca
     8341 tttagcgcat tagatccgat ttcaagaaac tcattacaag aattggtttt gtccttacac
     8401 gaagaattgg gcacgaccat cgttttcgtt acgcacaata tggaagaagc aatcaagcta
     8461 ggtgatcgta ttgcatttat gaaggatgga gaaattatcc aatgtgacac accagagcag
     8521 ttattgatga atcctaaaaa cgactacgtt cggcatttct ttgatgaacc gaaacagacg
     8581 aaagagtggc gagtagaaga tttagtcgtc aatggatatt tcttaaatga aataccagaa
     8641 aatgctcgtc aacaagtttg ctttgatacg cctatgaaag aagtatattc gcttctttcg
     8701 gtgtatccaa gcattgtgat agtagaaaag caaagaagta ttgggtcgct gacaagtaaa
     8761 gaaatctttg cttttttaag tcgagaggag gaaggaaatg aaaacatatg atgcaatcat
     8821 catcggttca ggtgtgagcg gcttaagtgc tgcttatgga ttgaaagaag caggtaaaac
     8881 agtactagtg gtcgaagaag atctatgggg cgggacttgt cctaaccgtg gatgtgatcc
     8941 taaaaaagtc ttgttaagtg cagtagaggc acgtaatcgg gtaaaacaat tgagtggaaa
     9001 aggttttaac gagatcccaa ctgccaactg ggaagaactt caaaaattca aacgaacatt
     9061 tacggatcca gtcccagaaa gccgaaaaaa acaattagca gaagctgaaa tcgatcatct
     9121 ttcaggaacc gctcgttttt tggatgattc atctattgaa gtcaacgaag aagtcttcca
     9181 tgcggattac ttagttttgg caaccgggca aagacctacg atcttaccag tggagggaaa
     9241 ggaatacttg aaaactagtg cagacttttt atcattgcct gtactgccta aagaaatcat
     9301 ttttatcggt ggtggttata ttgcttttga gctagctacg attgccaatg ctgcagggag
     9361 caaagtaacc attgtccatc ataatcaacg gccattaaaa gaatttgagg cgtctttggt
     9421 agaggaagcc gtccatcaaa tggaagcatc tggcattcag tttgcttttg gtgttgaaac
     9481 ccaaaagatc atttcagaag gtactcgcta tcgtttagtc ggaaaagaga cggaattagt
     9541 agcagatatg attttttgtg cgactgggag acagccgaat acagaatctt tagcacttga
     9601 gcaagcgaat atcgtctttg ataaacacgg aattgcagtc aatgattatc tgcaaacgag
     9661 caatccaaaa atttttgcgt gtggagatat tgtttcaagg aaaaccccaa aattgacacc
     9721 agtcgccact tttgaaggaa attatgtagc taagcggata acggatgcaa caagcgaacc
     9781 tatcaaatat ccgattatac ctacgatcgt ctacgccagt cctaaacttg ctgaagtagg
     9841 agttacaaaa agtcacgcct cttcgtcaga tcaagtggta gagatggatc ttaccagttg
     9901 gttcacctat catcgtgtca atgaaccagt tgctaaggct gaattgacct ttgaccagca
     9961 aaattatttg attggtgctg cagtgattag cgagcaagca gacgaattga tcgatgattt
    10021 gacactcgtc atcaatcaaa aattgactaa aaaagagttg gatagctata tcatggggta
    10081 tccgacattg gcaagtgatc tctcctattt attgaaataa tcagcatgat aactaaagcg
    10141 cttgttgctt tttccttctt gtgctacact ttaaaaaatg aatacctgtt tctggggatg
    10201 tcgtagacga catgagagta agttgtgaac ttagacccat tgaacctgtc agttaatact
    10261 ggtgaaggga gaaacatagc agacgcttga agcgggttac tatgtcgtgc ccatttcaag
    10321 cgttttttgt tattcatatt agaagaaagg aggtaacaaa atgaagaaaa atctttatcc
    10381 agcagtcagt atggcattca ttttaattat ttggcagatt gttgtcatag tttggaaaat
    10441 acctgccttt attttacccg gacctgctgc tgtattttct gcattagcgg cagatgcaca
    10501 agtattactt tcccattctg tcacgacatt aaatgaagca gtgcttggtt tggtaatagc
    10561 tagcgttctt tcttttatta ctgccttggc aatggattat tggaagtttt tagaaatgtc
    10621 tttttatccg ttactagtga tttcacaaat gttgccaatc atggtgttag ggccattact
    10681 taccctatgg tttggtttcg gtatgcttcc aaaagtgatc cttgttgtct tgataagcta
    10741 ttttccgatc gtagtttctt tttcggatac attgaggaaa gtatcaaaag agcaaatcat
    10801 ctttttgaaa acgatggggg cagatacagg gaagatttat cgaatatata aaataccagc
    10861 gggtttatcc ggcttctttt ccggtttaaa agtggctgct acttattgtg tcagcggagc
    10921 agtcgttgga gaatggctaa gcgctcaagc aggcttaggg tattacatga tacgcgtaaa
    10981 aaacagctac cagatcgata aagtttttgc tgcgatcatt tgtgtcattc tattgagttt
    11041 attgctgaat ggcgcttgtt cattattgaa aaaagggtat tatcgtatgc tttatatttt
    11101 ttaagagaag gggatcaaat gaaaaaatta tggcttttat taccgctact gttgctttta
    11161 tccgcttgtg gcactgcaaa agaaacaagt agcaaacaag aaataaagga tttgaaaaaa
    11221 gtgacacttg ttttggatta tgtaccgaat actaatcata ctggcatcta cctagcaaaa
    11281 gaaaaaggct attataaaga ggctggttta aatgtccaaa tcatcgaacc aggagataat
    11341 tcgacaagta ttggtctagt aggtgcggac aaagcacaat ttggtgtaag ttatcaagaa
    11401 gatgtcactt atgctcacgc agatggacag aatataccag tcaaagcaat tgctacagtg
    11461 atcaaacata atacttctgg attcgccacc ctatcagaca gtaacatcca ttcaccaaaa
    11521 gattttgaag gaaaaacata tgcaggttgg caaagtccaa gtgaagaagc tgtcttaaaa
    11581 gctgtgatgg aaaaagacgg cggggacttt tcaaaactga caatggtagg aagcaatgga
    11641 gaagggccag aaagcttagg aaaatcctca gatatccaat ggtactttga aggttgggat
    11701 atgatcaaag caaaagaagc gggtattgaa ctaaactata tcccattgaa agaattagat
    11761 gaacggttag attactatac tccggtaatt attacgaatg atcaactgat caaaagtgat
    11821 ccagaattgg tacagtcttt tatggatgcc acgaaaaaag gctatcagga agctattaag
    11881 gatccaaatg acagtgctaa actgttgcaa aaatatgcaa aagaaaatga tcggacgttt
    11941 ttagaagaat cacaagcatt cttgtcaaaa aattatacgg atgatcctga gaattgggga
    12001 ttgatggaag aaaaagtttg gcgcaattat acagctttta tgcaggaaaa cggattgatc
    12061 aagcaagatg taccttcgca agaactattc actaatcagt ttataaaata ggagtgatag
    12121 atatgtcaat ccaaatcagc cacttgaatg tagtcattga aaacctgaaa atattagaag
    12181 acatccattt cactatccct aaagatacct ttacatcgat tgtcgcgcca agcggagccg
    12241 gtaagtcaac attgctgaaa actttaacag gagtccagcc tatgacttct ggaacgatca
    12301 aagtagacga tcaaaaagta actggtctcc atacagcatt tagttatatg ccgcaagaag
    12361 atatgttact tccttggctg agcgtctatc aaaacgttac gttatatcaa aaaatcaatc
    12421 atctaacaat caatgaagaa caagtaaggg attatctgga tatctttggg ttaaacggct
    12481 atgaacattt tttgccggaa caattatctg gcgggatgaa gcagcgaacg gccttgttac
    12541 gaacaatcat gaatccttct tcttacttgc ttcttgatga accatttgga gcgttagatg
    12601 ctatgaccag agggcagatg caagactgga tgttgaagct gcctaaaaaa gcaaagcgga
    12661 ctactttact tgtgacgcat gatatcgaag aagcaattta tttatcagac aggattcttg
    12721 tgctttccgc ccgtccggca catgtgattg ctgagattca agtgcctgaa aaacgacggt
    12781 cacgtgaatg gctactgcag caatcagagt tgaagcaaac gatttatcag ttattggcag
    12841 gtgaattaga tgttaagtga tgctcattgt catttagatg ggagtcagag tttagcactc
    12901 cttcagcagg aacacacgat tttgacgatc atcaattgtg attcgccaga agaatggaag
    12961 gaaaacagac agcttgcagc aagtaagact caagccttaa gttacggtat ccatccttgg
    13021 aaagcagata gctatacgtt tgaacaagtt gagccttttt tgaaaaaagc aaggatcatc
    13081 ggtgaaatag ggttagataa tatttggacg aatgtaccga tgactacaca aaaaaaagta
    13141 tttgagcgac agcttgcttt tgcagccata aatgagaaac ctgttgtact gcacacaaaa
    13201 ggatgcgaaa aagagatctt aaattatatc caagcttacc ctaatcacta tttgatccat
    13261 tggtattctt ctttggacta tcaacaagac tatattgatt taggctgtta tttcagcatt
    13321 ggtcttgatt tgaaaaagaa tccagcagtc tggcaactag ctaaagcagt cccaatcgac
    13381 cacttactta ttgaaacgga tggactacat gcagcagagt gggctttagg ggggattttt
    13441 tcagaaaaag attatgtctc actgcttgaa gaacagttaa agacaatcgc tgagatcaaa
    13501 gagctatcag ttgaagaagt taaaaaaaat acttatcaaa acttggaacg tttcattaga
    13561 ttaggtaaat aaaaaagaga tgtgacacaa ctagtgtcgc acctcttttt ggctttcttt
    13621 gtataaaagt aaactgtttg attaataaat gaatcttagt accaaccgtt tgcttcccag
    13681 aatgctttcg ccgcttccca agaaccataa cgtgaagtaa catattgttc tgctactctt
    13741 tcttggtttg cagcagagta gtcaccattt aagtaagaag aatctaattg gtaacgtccg
    13801 atgtaacgtc cgttcgttgc tgtgtaagaa ccgcttgatt ctttttgtgc gatccattct
    13861 tttgcagagc ttgtgcttgc tggagtagca gcagtttctg ttacagcagg agcaggttgt
    13921 gtttcagcaa caggcgctgc ttgtttttcc gtttgaaccg tttcttgtac aacaggttgt
    13981 tcagcttgga ctggtgcttc ttgaacggct gcttgaggtt cagcagctgc ttgtgcagag
    14041 ccttctgttg ggattgttaa ttgttgtcct gagta
//
LOCUS       DAZKSU010000068        13948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000068 DAZKSU010000000
VERSION     DAZKSU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.069"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..310
                     /locus_tag="ACG8Z3_002108"
     CDS             137..310
                     /locus_tag="ACG8Z3_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824409.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            950..1423
                     /locus_tag="ACG8Z3_002109"
     CDS             950..1423
                     /locus_tag="ACG8Z3_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0824410.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1432..1659
                     /locus_tag="ACG8Z3_002110"
     CDS             1432..1659
                     /locus_tag="ACG8Z3_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824411.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2295..2666
                     /locus_tag="ACG8Z3_002111"
     CDS             2295..2666
                     /locus_tag="ACG8Z3_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824412.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2922..3164)
                     /locus_tag="ACG8Z3_002112"
     CDS             complement(2922..3164)
                     /locus_tag="ACG8Z3_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0824413.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3196..4098)
                     /locus_tag="ACG8Z3_002113"
     CDS             complement(3196..4098)
                     /locus_tag="ACG8Z3_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0824414.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4111..4299)
                     /locus_tag="ACG8Z3_002114"
     CDS             complement(4111..4299)
                     /locus_tag="ACG8Z3_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0824415.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="ACG8Z3_002115"
     CDS             complement(4313..4876)
                     /gene="amaP"
                     /locus_tag="ACG8Z3_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0824416.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4914..5807)
                     /locus_tag="ACG8Z3_002116"
     CDS             complement(4914..5807)
                     /locus_tag="ACG8Z3_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0824417.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5885..6808)
                     /locus_tag="ACG8Z3_002117"
     CDS             complement(5885..6808)
                     /locus_tag="ACG8Z3_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0824418.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6857..7207)
                     /locus_tag="ACG8Z3_002118"
     CDS             complement(6857..7207)
                     /locus_tag="ACG8Z3_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0824419.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7240..8142)
                     /locus_tag="ACG8Z3_002119"
     CDS             complement(7240..8142)
                     /locus_tag="ACG8Z3_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0824420.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8135..8992)
                     /locus_tag="ACG8Z3_002120"
     CDS             complement(8135..8992)
                     /locus_tag="ACG8Z3_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0824421.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9326..10135
                     /locus_tag="ACG8Z3_002121"
     CDS             9326..10135
                     /locus_tag="ACG8Z3_002121"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0824422.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10175..10672
                     /locus_tag="ACG8Z3_002122"
     CDS             10175..10672
                     /locus_tag="ACG8Z3_002122"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0824423.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11057..11130)
                     /locus_tag="ACG8Z3_002123"
     tRNA            complement(11057..11130)
                     /locus_tag="ACG8Z3_002123"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11094..11096),aa:Ile,seq:cat)
     gene            11317..11640
                     /locus_tag="ACG8Z3_002124"
     CDS             11317..11640
                     /locus_tag="ACG8Z3_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824424.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11804..12058
                     /locus_tag="ACG8Z3_002125"
     CDS             11804..12058
                     /locus_tag="ACG8Z3_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824425.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12128..12373
                     /locus_tag="ACG8Z3_002126"
     CDS             12128..12373
                     /locus_tag="ACG8Z3_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0824426.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12449..12814
                     /locus_tag="ACG8Z3_002127"
     CDS             12449..12814
                     /locus_tag="ACG8Z3_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0824427.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12875..13438)
                     /locus_tag="ACG8Z3_002128"
     CDS             complement(12875..13438)
                     /locus_tag="ACG8Z3_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824428.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4090 a         2577 c         2304 g         4977 t
ORIGIN      
        1 tttttcggag aattaaaaaa tacaataaat aactttagtt actttaatgc gttcttactt
       61 tatttttata ttatcatttt tcttattcac ttctttggtt tatactattc ttataaataa
      121 aaacgaggta tgtcctatga tgaaaagatt gtactattca ttgatcataa cgattggcta
      181 tttgattgta tcaaatctag gaaatatggt atttggtatc agtaaagaat tcagttggac
      241 tactacatta tgggaatctc tattcttttt catcttcgta tttttgttac aaaattatcg
      301 aaaaaaataa acatgtgcca tctatctgtt ggcacatgtt ttaggattta tggtactttt
      361 tattcggcaa tccattttat ttttctactt cttcttaata cctctaacac ttttgattta
      421 taaactaatc caatgcatct acttgcgctt attaagctat ttcagctaga tagtaccacc
      481 ccatgcgtca atgcttgatt ttccttttgt ataacattgt ataagcaata aaggacaaat
      541 aagtggtgat tacaccaaac cattgaataa gaaaaattct gatacgtaaa taaacatgaa
      601 cttgaaaaaa ttaatgaatc ggttagctac tacgtagttg tcttttttct catacagttc
      661 tgttaactta cacttctctt cgctagataa taaaaaatcc tccaactgta ttgtccaata
      721 taatcccagg catctaaact agcattatag aagagacaca catagaatct aaaataactg
      781 gttttaaatg tttcttacaa tcaaaaaaag ctgttaaatg cactccacac cttcatactc
      841 tatcattttt tataaacgat atatttttat tgtttttcga taaaaaatag ttgttttaca
      901 ataattattc tgctatattt tagctatcaa agataaaaga gggataaaaa tgaaatttaa
      961 aacattgcta ttaaaatttg tcgttttagg tataagtgcc gtatttattt tgttcggcgt
     1021 acttttttat actcagatcg ctacaagcga aaaaggattc aattttgatt ggcagaccac
     1081 attatttgtt tctgtcattt actttgccct tctcctaggg atcgttattg cttatttctt
     1141 attgaaacta ttgaaaaaaa tcgaagataa ccaagctttt tccactcaaa gcttaaacat
     1201 actaaaaaaa atcgaacgaa ccattttgtt tattggtttc gccttttttg gcttacttcc
     1261 taaatttatg aaagtagcgg atttagacga tgctccaggt ttgatgttgg taggaatcgc
     1321 tgtcgtattc attccttttg cagtatacac attaacaagc gtgctggaaa aactgcttga
     1381 acatgcgatt ttacttaaga cagatcatga tttgacagtg tgaggtgcga catggcaatt
     1441 atcgttaatt tagatgtagt attagctcaa aaaaaaatga gtgtgactga attgagtgag
     1501 aaagtgggca ttacgatggc aaacatctct attctaaaaa atggaaaagc aaaagccatc
     1561 cgtttttcca ctctcgaaaa aatctgccaa gttttggagt gtcaaccagg tgatttgttg
     1621 acctataaaa atgactcgaa tactatacca gaagaataaa ataaagtaga acaaacagta
     1681 aataaaaaat aatctgaatg atagatgtgt gagactttta gaagttattt acgtctatta
     1741 ttcagatttt ttatttcgat tgaataccgt tttatctata aataagatat gtactgactc
     1801 atacaaaggg ggccttttct tctccatgta ctatcacttt tcctgataca actggtttcc
     1861 tgttacgttg tagttggatc tgcttttagt ataatacttt attcattcca ccagactgat
     1921 agcagtttga gcagtctaaa taatcatgta gtattacagg ccattctgtt tcataccttg
     1981 aagtgcttaa aacttcagct aatggcccat tgtgttaact cttactccat tcttttttag
     2041 taagctccca tatatactgt aaagccttct atatattatg tcatgttgct ttttaacatt
     2101 tactatgtag ttttgataaa atgatttgaa taacagtgat ggacaattct tttttatttt
     2161 taacattatt ttgataataa tgattattta tttaaaaata cattagataa catttgttac
     2221 gacacgaaaa agcttcacaa acaaatatcg ttattttaaa atgagggcga actcacacaa
     2281 ttaggaggaa tagtatgcca tttattgttt acttttttct ttcattactc gtgatctata
     2341 ttccattacc aacatttttc ctgctcgtca atcgtctagc agagcaacat gatacgattg
     2401 ctgctcttac gaaactttgt ttacttctat ttgtttgtct agattacttt acgagctatt
     2461 attggctaaa acagacaaga gtaaaaaaga gatattattt cttccttgtc tttttcaatc
     2521 tctttgagtt tctgatccat tgctttttac ttcatcagta tcatacgatc catttgtgga
     2581 tcatctgggt tttcaaagtg gtcgtattat tttgtatgtt cacctttcct atctcaagaa
     2641 aatttaggga ggcattgttt tattaaagaa gcttctctgt atcaataaag acacatttaa
     2701 ggaaaaactt ttaaaaatct aattgggaat ttgcccgcat aaataagagg ctgtgacaaa
     2761 agtcttgtca cagcctctta ttccgtataa acagtgttca aaagcttgca tcttcgataa
     2821 tatgcccaac caaatgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
     2881 atgaagaaca gctttttcac gacctctttt taaaagtctt tttacagttt tcgagcaaag
     2941 aatgatacga ttagtactaa aatgatggca ccaatcaatg atggcacgat tgccattcct
     3001 gcaatacttg gtccccaaga acctaataat gcttgaccaa gtgctgatcc aactaaacct
     3061 gctacgacat ttgcaataat ccccatcgaa gaacctttat tcgtgattgc tcctgcaatt
     3121 gctccgataa ttcctccgac gataagtgac caaataaatc ccatgtttcg ttcctcctaa
     3181 tgttttactt ccttattact gaacccgtgg ttctgtattt tcttttactt tttgtgcgcc
     3241 gcttccagct gctgatttta ctttatctgt ttgattcgaa gcaaattcac cggttttttt
     3301 cgctgcgtca gttactttgt cttgcaccgt ttcttgatct ttttcaaatt cttcttttgt
     3361 tttgatgtcc actacgttag cgttgacttc gatcacttct aaatgtgtca tattttttac
     3421 ttctttgccg attacttctt gcatttcttc aaagattttt tcgatatctt tgccatattc
     3481 cacaatgaca tccatatcaa ctgctacttg ttttttaccg acttctgttt cgatcccagc
     3541 cgttacatta tctgtattca caagttttcc tgccacatta gagaagaaac caccgtttgc
     3601 agataataaa ccgtctactt gttcaattgc gattccgatg attttttgca caactttatc
     3661 ttcataattc agctcaccgc gcattgaatc ttcctgttca gaatcatgct ttccttggtt
     3721 atttgtatga tcagaaccaa gttttccttg tccgtttgtg tggtcagaac taagtttttc
     3781 ttgtgctttc gcatggtcag aaggatgttt ttgctcttcc acaggtttag aaggatcgat
     3841 tgcatcatgt tgttctggaa ctttagattc tggagttcct ggatgatgag gtgcatgctt
     3901 ttctgcatca tgtttcttct cttctactcc ttgttgaacg ttagggacta ctggatctgg
     3961 atgtgcagat actggtgctg ttttgttctt ttcatcgttc acaccatttg attggtttgg
     4021 tgtcgttggg tctggatgtg cagacactgg tttttggttg ttcattttat tgttattatt
     4081 tttgttattg ttattcatgg atgtttcctc ctaagattta aaatagtgat ctaagatttt
     4141 cgtttgtttc agataaaaac cgatagcgac gccgatcgct gccatgatca agatcaataa
     4201 ggtcttgaaa aagcccacgg tgatccataa aacggctaat atcaatccaa gtaccccaaa
     4261 gatgataggg agcttgtatg tcgaaaatag ttctttcatt tttggtcccc ccctattcta
     4321 ctcgtttgcg gtcattgctt cgtttagctg gttcttcaaa gcctgtgtat ttcaccgata
     4381 ctttcactgg ctcttttgtt cccaatatgt gttgtacttc attttcgatt tctttcatca
     4441 atgttcctgt tttaccaatg agtgctgaag ttcgtttcaa ctcccctttt acattgacgg
     4501 cgatcttgtt tttcgttgct ttgacattta cttttggtga atggatgaat tcatcttcat
     4561 gcaagtgaga acgaaccatt ccttcaattg cttttttatc caacgtcaac tccccatcac
     4621 catgtttcag tagaaagttt ccgattgttt ttggataaaa caggaaaaca agtaacgtga
     4681 taattgtcaa aatagcaaat gcaacggcaa gccaaaatac atattgttgc acgtatgaat
     4741 tagtgatgga aaagaatcga aacttctcta gctgaaaagg caatggatag gttgcactta
     4801 atagtatcgc aaacaaaagc acagatagca aaagtaatgt tccgatcact ccgatcgttt
     4861 ttacaccttt attcattttc gcacctcctc ataggggtga aacgatcaac ttcttataag
     4921 ttgatcgctt ctccaccagt tactccgtaa atttgggctg taacataact tgctttattc
     4981 gatgctaaga aaacataaac tggtgctaat tctcctggct gacctgcacg ttctagtaaa
     5041 ctttgctgac caaattcagg gatcgaatca gaaggttgtc cttcgtctag ttgtaatggt
     5101 gtccagattg gaccaggtgc tacaccattt acacggattc ctttaggtgc aagctgtttt
     5161 gctaaaccga tcgtaaagtt agcgattgaa gcttttgttg ctgcgtaatc taataaatgt
     5221 tcgcttggat tgaatgcttg aacagaagtt gttgttacga ttgctcctcc tgctgggata
     5281 tgcggaacag cttctttcac taaagcaaac atagcgatga tgtttatcat aaatgtgtct
     5341 ttgacttgct caagtggtaa ctcagcaata gactgacaag aaatttgctg tcctgcgttt
     5401 agtaccatcg tatctagccc gccaagttca ctcacggctt tatctatgat ttctttaggt
     5461 gcttgttcgt cacgaagatc aaaaggtagt aagacaatct tacgtcctgc aactttcacg
     5521 tattttgcta cttcctctgc atctttttct tctcctggga agaattgaag agcgatatct
     5581 gccccttctc tagcaaatgc aatcgctgct gcacgaccga tccctgaatc tccaccagtg
     5641 attagtacac ggcggttttc tagttgatta ttgccttcat aggattcttc tccacaatca
     5701 ggaacaggaa tcatattctg ctgtaaagct ggtgtatcct gatcttgttt aggaaatttc
     5761 tccgtatggt acaagtttcg cgggtcgatt aattttggtt ctgtcattgc tgtattcctc
     5821 ctagttttta aattgtattg ccaaaacaaa tttctttgtt tgatacctgt tttaaataga
     5881 ttctttaatt tgtataatct ttccggtata aatggtaacc taaaactact gctgctatca
     5941 aagagccaaa gactacgagt ataaagccta gagcagaact tttacccggt aatcctcctg
     6001 tattcattcc ccaaatccct gcgaacaacg ccggaacgga aatgattaat gcagctgaat
     6061 ctagatattt catgatatga tttagattat tgtccatcat cccgttgaat agatcaccaa
     6121 tactctctaa taaatctcga tagatctcga tcattttttc tgtttgtcgc tgtctcaaat
     6181 ggacatcata gagtagctgt tcatcgtcta aacgtttgat gaaagtttca gaattccata
     6241 attgatccat tgtttcccgt tgatctctta aggtatgatc gatatatacg atgtctcttt
     6301 caagatctgc ctgtttatac agttcttcat tttccgttgt tttacgagct gctttatcca
     6361 aagcgtcaat tcgctttttc atctctctta gttctttcac gtaatgggtg taaatatcta
     6421 ataatgtata cgtgatgatc ttttcaaacg agtttaaatg atttccatgc tttgtcagca
     6481 gccgatccac aaattcggtt tttctatcga tacaagtaat caagagatta tcggaaagta
     6541 caaaagatat tggcgttaat tttttttcga tacgattatc tgactgatct agattaatca
     6601 ctaccaaaga aaatggatgc tctagtttcg tctcataaag gcgctccatc cgagaaactt
     6661 cttccggata ttcggttcct acgaaaatat catcaggaag ctcgaattct tcgattgctt
     6721 gctcaatctc ttcgggcttt gcactatcaa gtacgagcca attatatttt ttttcatctg
     6781 tttctattac attcttttct tcatccacaa agtagtatct caatgtatac ctcctttttg
     6841 gactttctcg aaactattag ctgaagaagc agttctttaa tttgatatta taatcgagta
     6901 ctgcgatttc attttctaat gaaaagctgt caagctgttt gttgaagtcc atgttcaatc
     6961 gtttaccgaa ataattttgc tctaatttag atgtatagat gtccaataat ggatgctcga
     7021 ttttagtcgc atattctcca aattccgaaa caaaaggaat gatttgaaaa aagctgcctt
     7081 ttgcaaaagc acctttgtcc cttagaaatg gatcggaatc attgataagc gcgagtgcga
     7141 tttttgtatt ttccccaacc ttttcaatta acgtctctac tgcattttta gacattgtaa
     7201 tttccattcc tactccccct cttcagttgt tgtcttctat tatttaacaa ctacatctgt
     7261 ttttggttga acttttgcat attttggata tttagggaat ttagctacac aacggaaagc
     7321 cgtcatccct gcatattttt cgtaatattt cttcgttggc ccttcaaaat ccttgctcca
     7381 cggtttcaca tgtcctgaaa aatggataat gtaaggattg ttgcgagttt cttcgtattc
     7441 acgttcacct gctgctgttg gatgtttttt tgcttttgcc atgatatagc cttgtgcatt
     7501 ccaacgcgga tgaagtggta accaacggtc atgtaagata gcatttaatg catcttggtc
     7561 atggaatcgt aatttttctg gattatgttc gatgaaatct aatacttttt gcgtaatatt
     7621 ttcatctaac cattttttca cattgatcag catcaaacct gagttgaaat aacgcattga
     7681 tttagctgaa attttcattt tttccagtcg ttgatggaac ccagcgtctt caacagctgc
     7741 tacgattgaa tcaccgaagt caagacgcca taatttagag atatcttgta aagcgatcat
     7801 gtcgcaatcc atatataaga tacgttcaac ttctgtgccg cggaacaatt ccggaatcgc
     7861 aatacgataa taagctgttt ctggaatacg atcgctgatt accacatttg taaagaattc
     7921 tttattgatt ttcaagaatt ccacatctga gttcattcgt gcgtttttca ctgagaagcg
     7981 taaaagctgt ttactttcaa aatcgatatc atcgtcaatc acatagaacc gaacaaattt
     8041 agacgggttg caattatcta atactgagac aaataatgca gctaaatgtg gtacgaattt
     8101 tgtgttacaa cttgcgacta ctgctatttc ttttttattc attgatctcg cctactttcg
     8161 ttagagtact gtgtgctaat ttttttaagt ataaattggt atacggatga tctttcaacg
     8221 tattccaagg tttatcatgt cctgtaaagt gaacgatcga aggtttttca tttccttctt
     8281 tatactgacg ttcatatttt tcatttggtg ctggatgacg ttcaaaaatc agcgaagtct
     8341 gcatgttcca ttttggatga agttgttccc aatcttcata aagtacagca ttcaaagcat
     8401 cttggtcgtg atagatgatt cgatcgccat tttcactgag gtaatggata gttttttctg
     8461 taatttcttt tttattccat tgatcaatgt caatcaccat cactcctgaa ttgaagtaca
     8521 agtcatcgga atcgattcct aatctttcca aagctttcac ttggcctgga tcgataatag
     8581 cgccgattgt ttttccattt aacggctcat catatagttt tacgatatcg tctagtacta
     8641 acacatctga atctaagtaa aggactttct tataatcctc tttagctaat aagtttggta
     8701 aagaaattct taagtaagcg gtagttgtga tatggtcact gactaggaag tcttcgtaga
     8761 tatctttttc cactgtcagg aattggatac ttgcattact tgaatattta tttaccgttt
     8821 cttccaatcc ttctttacta tattctgata gaccatcgtc aatcacataa aattttatgc
     8881 gtcttgtttt attgcaattt tctaatgcag ttgcgatcat cacgctcaag taaggagcat
     8941 aattttcgtc tgaagcagtt accacaggaa ctacgccgta tctcttctcc atcaaaatca
     9001 ctcctttttc taatcttttt ctaacctgtt ctacatctag aatattcctc atttggtttt
     9061 aaagcaaaat atatgctttg ttttcattga atattttaaa atttttttat cacaaaacgc
     9121 atcggtaagc gctaactatt cgtttttcat ggctttttat tcaaaggtat tgaccaaaaa
     9181 ctactatata tagccacctt tttcttaaga atgttttttg aaaaaattca taatgacaaa
     9241 gcaagttagt ttcatttttt taaattctca tttaatctta aggtaacagg aaaatagata
     9301 tgtaaaaaga aagtaggaag ataaaatgaa actagcagcg attgatttag acggtacctt
     9361 gcttgatcga aatggaaaga taccagaaga aaatatccaa gcacttcgga aatttgatga
     9421 aaatggcgga atcgtaaccg tcgctaccgg aaggaacagc atttctgcta aagatatttt
     9481 ttccgaatta ggaatcggtg gctatttgat ttcttccaat ggttcactga ttgctgaaat
     9541 gaaggatgga aaaatggcga aagttctcag acgttcaaaa atagatagcg atacactgag
     9601 aaaagccttc ttctatgctc aagaagcgaa aatatccatc attgctagcc gagaaaccca
     9661 agatgatcag ctgacattcg atgaatcaaa tctagtcaaa gatgaccctt attatgaaca
     9721 tttcaattta cagaaccatt cttttgatca aattttaaat atgttggaag atccttctct
     9781 tagctatctt aaattagcat taacagataa gagtgaagaa aaactacaga agctacaaag
     9841 agatctgcaa gcagatggta tccaatcagt attctctgat ccgcatttct tagaaatcac
     9901 gcctacaggt atcactaaag ctcattcact tgtttttctg gcggattacc ttggaatcga
     9961 atcgagtgac atcatggctt ttggcgatca agaaaacgac attgctatgt tgaattacag
    10021 cgggttgagt attgcgatgg ggaatgcaca gcgacaagta aaagaaattg caaaccaagt
    10081 aacactgacg aatgaagaag ctggtgttgc ttatatttta aaaaacttct attaaaaaga
    10141 ataaaatgac gatatgaatg taggaggaaa gaaaatgtta gttattattg atatgcaaaa
    10201 tcatattctt gatccaaaca gcgaatttta tattccagag tcagatcaat tggcagaacg
    10261 tattgctcaa agattagcta aagcacgcca aaataatgaa tatattctct tcacacggga
    10321 tataccagtt gaactgaagg acaaagaaga agaacgagaa gatttgcaat tgatccctct
    10381 tctttctcca caagaaaatg aacgagtgat taaaaagtat tactttacga ttccacctga
    10441 aactttagct gaaatccagg aaactttatt tgaaagcaaa gaagaacaaa aagagatcga
    10501 agtagtcggg attgaaacaa acttgtgtgt actctctaat ctgataggcc tgcagagtgc
    10561 ttttcctgaa gcagactttt ttgttgatcc aatgttagtc tccagtagaa aacacggaga
    10621 atccgcttta gagctgttga aagatttcaa cgtctctatc gtcgaatcat aaaaatatct
    10681 atcttcattt acgatattta acacacaata aaaaaagcac gcagaactgt tttagtccta
    10741 cgtgcttttt ctatcaaagt agattggctt ttttatgaca cagagtgaaa cggcaggctc
    10801 acgacctctt tcttaaatgg tgttcaatca gctgaccctc ggtattagct taacaaacgg
    10861 caaaatatga ggaactattt ttccgtttgt taagctaata ctcaggtcaa aacgctgatt
    10921 tcacaacctc ttccttaaac ggtattcaaa agcttgcatc tgcagtaata agaacagcca
    10981 agtaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcagct
    11041 ttttcacaac ctcttatgta ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
    11101 gtctgctcta accaactgag ctaaaggtac atcataaata aaatttattt ttgttacttt
    11161 tcaactatag cacgaatgac aaactctcac aagtttcaaa atataagtaa aataatattg
    11221 ttattcttat ttcttttact tgtgtcataa ctattttgtt actttaattt ttatttgctt
    11281 attttagaaa aaatgttatt cttttattta ttctcaatgt taggagtgat tattttgcca
    11341 tttattgcga ttttattcga tttagttgcg gcagcagctt attttctcca attaaattat
    11401 caaacctcag gaattctgtt tattggaatg atttttcaag gtgtgattac ccttttgctt
    11461 cttgtcatga tgctcagcta taaaggaaaa agatatgccc gtattcaaac aaaagtattt
    11521 gttaaatatg tttccattcg ctatgggatc atcattcttt cctttttagt gaatgcgatc
    11581 gtcctctttt tatatgtatt gaattactta gatatcaatc cattgatttt ttctcgatag
    11641 ataagacaca ttcagcggaa cagttcctgc ttatcagcag aaaactgttc tgctttttca
    11701 tttttcattg atctattctt cttttatttt cctagcattt catttgtgat gtatgcaata
    11761 acatgttatc ttttattata tgaatcgttg aggagtccgt tacatgatta cgttggtgag
    11821 agttttgttt tgggtgcctg cggtcgcact ggtcgcaagt attgtttatc ttatgaattg
    11881 gaacaaagaa cggttttatt tagctatttt gactcttccg gctatctact ttatgtggaa
    11941 agtttttaat tacaactact ttgaacccga tagtgttttt atagaagaat tatcaggact
    12001 ggttttgtct ttattgatcg tcattcttta tttgatacgt ttaaataaaa aacattgagt
    12061 actattgatt gcatgtgtag ctttctatag tcattataac tattctacta taatgaaagg
    12121 tgattaaatg agtatcgaag aagctatttt actggatact ttacgttgtc gtctgaattt
    12181 cttaagaact ggccgatctt tatctcaagt agctgtttct agcgcgttaa gtattccttt
    12241 attcaaatat cgtaagtacg aagaaggaga agaaattcca agcatcattg aattaatcta
    12301 tatcgctgac ttttacgata tttccgttga ttacctaatt ggtcgctcag aaacagcaag
    12361 caaactgaag tgattttttc cagccttggt gctggttttt tcatttaacg tctaaggtta
    12421 acaatctata tataaaggag acatttctat gcgattatgg catgaaaaat tgatttcaaa
    12481 gcttcctcgt caacagcttt taggacaaca tcgggaatgc tgtgcattaa gaggaaatgg
    12541 ctggggaaag aaacatgcta ctgtggatta tgtttttcac tattctcctt atagattatt
    12601 ccaatatcat cagcttgtca tggatgaaat ggaaaaacgt ggttacagcc cagcaccaga
    12661 gtggaaaaat cctatgtatc gtggcaaatc atgcgaagca ttttttgatt tacctactgt
    12721 agagcgcaac gtgccgattt atccggaaca cgatgaagtt tatctagcag aatgtttagc
    12781 aaatttggag caaaaaggaa tttatcttac ctaatataac tagaaaagcc ttctatctcc
    12841 tcttacttca ttaaaaagct ttaaccatct tagatcaagc taaaatgtcg taagttttag
    12901 ggttatcttc atcctcacgt gcaaaactaa aagcgtactc tagttttggt tcatcgccaa
    12961 cttccctaga cataaatgaa catctcctga cgtggtttaa aagtaccgca tcttcgacgt
    13021 ctgtatacga tgcacagcac agattgcttc ttttgatctt tcttttgatt tttctgttgc
    13081 ttcttctaca ccttcttcga ttactacatc gatgcttaca attgcttctg cctaatattc
    13141 ccatgcatcg tcgatttaag cctctgtagt cactgtgatc gtttccacat tttaactcct
    13201 ctgtcgtggc ttcagttgat tttgtgatca gttcagttgc taagccagag tcagaacaac
    13261 ctgccattgc tagcgttaat attgatagta aaaatagttt ttttatcata aggttccccc
    13321 cctgaaaata gactaaaata ataacgcttt ctttttttgt tttcagacga aaattaataa
    13381 tttctaaata ttctttaaaa aagcttataa ctattatgcc ttttttaata tataacacag
    13441 ttatttttaa aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata
    13501 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
    13561 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
    13621 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
    13681 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
    13741 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
    13801 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
    13861 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
    13921 attcagccca ttcaacgacc aatgaatt
//
LOCUS       DAZKSU010000069        13917 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000069 DAZKSU010000000
VERSION     DAZKSU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.070"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG8Z3_002129"
     CDS             131..505
                     /locus_tag="ACG8Z3_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0824429.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG8Z3_002130"
     CDS             complement(619..1248)
                     /locus_tag="ACG8Z3_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0824430.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG8Z3_002131"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG8Z3_002131"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="ACG8Z3_002132"
     tRNA            1668..1741
                     /locus_tag="ACG8Z3_002132"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="ACG8Z3_002133"
     CDS             complement(1879..2043)
                     /locus_tag="ACG8Z3_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824431.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="ACG8Z3_002134"
     CDS             2338..3666
                     /locus_tag="ACG8Z3_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0824432.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="ACG8Z3_002135"
     CDS             3868..4701
                     /locus_tag="ACG8Z3_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0824433.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="ACG8Z3_002136"
     CDS             4717..5454
                     /locus_tag="ACG8Z3_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0824434.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="ACG8Z3_002137"
     CDS             complement(5609..6577)
                     /locus_tag="ACG8Z3_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0824435.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="ACG8Z3_002138"
     CDS             6809..7642
                     /locus_tag="ACG8Z3_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0824436.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="ACG8Z3_002139"
     CDS             7676..8515
                     /locus_tag="ACG8Z3_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0824437.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="ACG8Z3_002140"
     CDS             8541..9101
                     /locus_tag="ACG8Z3_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0824438.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="ACG8Z3_002141"
     CDS             9300..11021
                     /locus_tag="ACG8Z3_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0824439.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="ACG8Z3_002142"
     CDS             11186..12328
                     /locus_tag="ACG8Z3_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0824440.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8Z3_002143"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="ACG8Z3_002143"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0824441.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAZKSU010000070        13602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000070 DAZKSU010000000
VERSION     DAZKSU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.071"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..1223)
                     /locus_tag="ACG8Z3_002144"
     CDS             complement(117..1223)
                     /locus_tag="ACG8Z3_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0824442.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     regulatory      complement(1397..1563)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(1788..1994)
                     /locus_tag="ACG8Z3_002145"
     CDS             complement(1788..1994)
                     /locus_tag="ACG8Z3_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824443.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2010..2624)
                     /locus_tag="ACG8Z3_002146"
     CDS             complement(2010..2624)
                     /locus_tag="ACG8Z3_002146"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0824444.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2771..3454)
                     /locus_tag="ACG8Z3_002147"
     CDS             complement(2771..3454)
                     /locus_tag="ACG8Z3_002147"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0824445.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3563..4021)
                     /locus_tag="ACG8Z3_002148"
     CDS             complement(3563..4021)
                     /locus_tag="ACG8Z3_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0824446.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4105..5868)
                     /locus_tag="ACG8Z3_002149"
     CDS             complement(4105..5868)
                     /locus_tag="ACG8Z3_002149"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0824447.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6089..7549)
                     /locus_tag="ACG8Z3_002150"
     CDS             complement(6089..7549)
                     /locus_tag="ACG8Z3_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824448.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="ACG8Z3_002151"
     CDS             complement(7757..8683)
                     /gene="glsA"
                     /locus_tag="ACG8Z3_002151"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0824449.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8706..10133)
                     /locus_tag="ACG8Z3_002152"
     CDS             complement(8706..10133)
                     /locus_tag="ACG8Z3_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0824450.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10164..11378)
                     /locus_tag="ACG8Z3_002153"
     CDS             complement(10164..11378)
                     /locus_tag="ACG8Z3_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0824451.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(11464..11691)
                     /locus_tag="ACG8Z3_002154"
     CDS             complement(11464..11691)
                     /locus_tag="ACG8Z3_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824452.1"
                     /translation="MKNKFENRSDTRCGSFLYILYEVFFIPYEKISLKIKKDENKRFS
                     IKRRPFIFCLYLCEKRKRAQHIKLLLIVCLL"
     gene            complement(11719..11961)
                     /locus_tag="ACG8Z3_002155"
     CDS             complement(11719..11961)
                     /locus_tag="ACG8Z3_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0824453.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(11993..12550)
                     /locus_tag="ACG8Z3_002156"
     CDS             complement(11993..12550)
                     /locus_tag="ACG8Z3_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0824454.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(12563..12751)
                     /locus_tag="ACG8Z3_002157"
     CDS             complement(12563..12751)
                     /locus_tag="ACG8Z3_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0824455.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(12764..13330)
                     /gene="amaP"
                     /locus_tag="ACG8Z3_002158"
     CDS             complement(12764..13330)
                     /gene="amaP"
                     /locus_tag="ACG8Z3_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0824456.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4067 a         2810 c         2221 g         4504 t
ORIGIN      
        1 atatgatttt ttgagatact ttcattgcga attcctcttt ctaaaagaac ctctcgcttg
       61 aaaaataaaa gattgtactt tatgataatt caaactgaga gtttctcagt ttctctttat
      121 tgtttttcag gtcgatagag cgccaactct tcggcctgta tctttgaaga aatattcaac
      181 gtgattccga ttcctaaaga aaggatcaga taacttgttc ctccgtagct aacaaatggc
      241 aatggcacac cagtcattgg catcagcccc gcgatacttc ccacgttcat tatcgtctga
      301 acgaatagca aagttccgaa tcctaaaaga aacaatccag cttgctgatt tttacagcga
      361 ctgcttaaac aaaagattct catgcataat gagaacaata agaaaagcac acacaatgca
      421 ccgattagcc caagctcttc tgtaataatc gaaaaaataa aatctgtttc tgtctctgga
      481 agataccctt tttttgtgat actatttcct aatccccgcc caaatatgcc accattatat
      541 agtgcataaa acgaatgggt catttgatag cccgcaccat gactatcgat aaaagaatct
      601 cttaaagtcg cgatcctttc ataagcatga ttaaacatct gtggaagcca acctttattt
      661 cctaaatata ataccccgcc tgctgctcca atcagcaatg cagaaaaagt aacgattaga
      721 tagatccctt ttttaaatgg aaccgccgct gcccaaaata tgacccacgc aatcgaaagg
      781 atcatcaatg ctccagcaat ctttggctga aataaaatca gaaaagttat acctactgta
      841 atcaggaatg gtttttttag atcttttggg ctactatttc cgtcacgaaa aaaccagctt
      901 aaataaaaaa tcaaaaaaag atttgccagt tcagaaggct ggaattgaat gccaaacaga
      961 gagatccaac gttgtgcgcc gttgacagtg acaccgaata tccctattct tactaatact
     1021 aaaaagaaaa tcgataaggc taaaccgtat cctgctattt tagggtgaag taaatagtgt
     1081 agtttgactg aacgagctaa gaagatcacg ctccaactca aaaatatgaa aattagttga
     1141 cgcaataata gactttttgt attttcatcg gcttgcaata aacgataaga actggcactg
     1201 tatacttcta aaagaccgat catggacaaa gtcaaataag gacccaaaat ccaccaatcg
     1261 atcttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg aggagcgagt
     1321 ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa ccttttcgaa
     1381 caagaattct gacctagcat catctcatcg tccgagtttt agcactatac aacgtaaaga
     1441 aaacattctt agctattgga aaaagcctta atccaacagt ttctgtttta cccattggcg
     1501 actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct aacaaggtta
     1561 ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg caatggaatt
     1621 tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa taacaaataa
     1681 gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga taatttttta
     1741 gttttatgtg tttttttata tgttttttca cttttcggaa taaatattta tttgttaaag
     1801 taattaaaca atccgctaat cgtttgatct ggcatttcta tttccttacc atctaaagta
     1861 acagtagtaa cgacaaaatg gtcttgcaat ggcggaactt ctaagtactg cccttgcaga
     1921 aaatcattca ctgtttcata ttcttttttc atttcttttg aatctgtcga tggtttatag
     1981 cttattttta acataactag tgctcctttt tattcgggac gtgcaatcgt gaaaatttct
     2041 cctattgctg cataatcatt tcctgccaaa cggcttacag cattcaattt tcttggatcg
     2101 atccttcctg tcttatcatc atagactgtt tcatttaaat gatatttctg tacttctagc
     2161 aaaaataaat cagctgttac tttattttta ttccgtatct cgacatgttc atataaggat
     2221 gtttcaaaac gaacttttgc ttctgtcaat gctggtactg aaatgattac tgaatcagtt
     2281 aaagtcaagt cagtcaggta cagttcgctt tcctcattag gcagattggc tgctgtttta
     2341 tttacttctt ctacgttgtc ctcatcaagg atatggataa cagcttctct tgtctcaaga
     2401 atattctttg ctgtgtcttt tactgcatct tgttttctct ggatcgccaa ggataagatg
     2461 ggtcgattcg aactgacgat attgaagtaa ctgaagggtg cgatattcac tatatctttt
     2521 gctgatttag tcgttactac tgctactggt ctagggatga ctgaaccaat cagcaacttg
     2581 taattttcac gttctgtcaa ttctgaagga ctccgttcaa acatggtttc gccttctttc
     2641 tataaaattc gttaaaaagc agatcttgaa gacaaaatga aaagtaatgc ttttttcatc
     2701 ttcttagaat ctgctttaaa ctatctatca atttttcttt tttgtcaaga atctgtgccg
     2761 tgcgtgtttt ttagttattt gctttacttg cggcttcgac atatctttta gtgatcaatt
     2821 gcggacgggt gataatagat ccaactacga ctgaaaaagc acctaattca tatgctttct
     2881 ctacttgttc aggagagtca gtatgtcctt ccataattac tgggatatcg acttgttcta
     2941 tgatttgatt caataaatca aaatcaggtg tctgcgtctt tcgtgaatat tctgtatagc
     3001 ctgctaaagt cgtagacaaa agatcaaaac catcttcttg cgcttgaagc gcttcctcaa
     3061 aagtcgaaat atctgccata gctaaccgac cgtgttcgtg aatcatttgg acaagttcag
     3121 cagttgtttt ttctgtcgga cgtttacgag aagttgcatc cagtgcaatg atttctgctt
     3181 gcgtctccaa aagttcttcc acttcttttt ctgtcgcagt aataaacact tctgacccat
     3241 cgtaatctct tttgataatc cctattaccg gtaaatcaac gattcgcttg atttcattga
     3301 tatcttctgt cgtatttgct cgaataccaa ctgctccgcc ttcttttgca gccaaagcca
     3361 ttctccccat aataaaagaa ctgtgtagcg gctcgttttc caaagcttga caagaaacga
     3421 cgactccgcc tttgatttgt tccagtgtat ccacaatatc tcctccagta atgttatata
     3481 aaaatacagc ttcatataaa aatgtaaccc ttttctattc aggagacaat aatgagctat
     3541 ttctttcagc agacctttct tactaaaaga aaattacttc aaattcttcg tatacgacca
     3601 gcttctcttc tgtaaattct atgttcaacg cttctttata agcatgttta aaaaactcag
     3661 catggacttt ccgccaataa gctaatgtcc gatccccttc tccttctttc caagcatgct
     3721 ctttcgtgac ttcactaaaa ggcaagatgg tcacatttat atttttgatg atacaaactg
     3781 gttcattttt tccattcagg accacgtcta attgaccaac ttttggtaaa ggctcattct
     3841 cttgtttata cagatcgtaa ccacttgatg taccagtctt tatctttttt acaactagtt
     3901 cgctcaactc gtctgccatt ttttctgtat ccccaaatga aaagaaacct aatttttctg
     3961 attgataact aggatatttt tcttggaact ttttgacata ttcttctact tgctgataca
     4021 taacatccac ccctttgctt tttataatga aaaaaccatc cagatactag tataaacaat
     4081 tatctggatg gtgagcataa tgaattattg aatattcaag acacgcagac tatcttcaac
     4141 gatttgtttt ggtgttaaat gataacgttc ataaagctgc cctacaggaa cgctatccgt
     4201 gaattctttc tctgcaccaa aatttaatac gttcattgaa gaatcaccat aataacgact
     4261 gatattttcg ccaaatcctc ctgacaaact accatcttct aatgtgatga ctaattcgtg
     4321 tgtttctttt aatttatcta atgtttctac gtcattttct gtgatcgttc ctggattgat
     4381 caacgttgct tcgattcctt tttttctaaa agcttttact gtttcttttc ctaattcaag
     4441 aaaaccgcct aaagctaaaa ttgctacttt tcctccatta tttactgttt gataggctgg
     4501 aatactataa tcatttgcga tagctgtacc atgttctaca ccatgtgtag gaattcggat
     4561 tgctacaggc tgcgctgttt gttcgaacgc ccaatctagc atagagatca tatcctcttt
     4621 tgttgctgga gcaagatatt tgatgttcgg gatacttgat aagtaaggaa tgtcaaatgt
     4681 tccttggtgt gttcggtcac tagctgagat cgttccgcca ccgactaaaa taatcactgg
     4741 attctcgttg atcgctaaat catgtgataa ttgatcatat gcccgttgca agaaagtact
     4801 tgattggaag acgactggac gtactccctg agaagccaaa gcagaagcga aagtgataga
     4861 gtgctgttca gcgatcccca cgtcaaaata atgatctgga tgtttttctt ggaatgtttt
     4921 cagtccaaag gcgcctggta tcgctgcatt gactgcggca ataggagctg tacttgtttg
     4981 cattttctta tccaaataat ccatgatgat catattaaat gtttctccag aagttacttg
     5041 ctttgcctcc ccggtggaca gatcaaatgg tacatgccaa tgatatttca ttttattatc
     5101 gattgccggc tgataaccgc gacctttttc cgtatggacg tgaagtacaa tcggatgatc
     5161 aatatctttt acttcttcaa ataaacggat caccgtttcc agatcgtttc cattatccac
     5221 gtaacgataa tcaaatccca ttgctttaaa taggttattg ctgcttttac cgttggtttg
     5281 acgaagctca gctaagtttt tatataaact gccatgattc tcatcaatag acatctcatt
     5341 atcgtttact aatacgatta gattggattg taaaacacca ccattgttca atccttcaaa
     5401 agctaagcca ccacttaggg agccgtcacc gataaatgct accacatttc ctgattcttt
     5461 cttcagatct ctcgctttag ctaaacctgt tgctaagctg atagatgtag atgtgtgccc
     5521 cactgtaaaa tagtcatgtt tactttcatg ttggttggaa tatccgctca cttcacggta
     5581 atgtcctttt tcaaaaccat tttttcgacc agttaaaagc ttatgaccat aagactgatg
     5641 ggaaacatcc caaacgattt tatcctttgg tgaatcaaat acataatgaa acgcaatcgt
     5701 taattctact acacctagat tcggaccaac gtgcccacct acatcgctgt ccttttgcaa
     5761 gattttttgg cgcatctctt ctgccaactg tttcatttcc tcaacagata attttttgac
     5821 atctgcgggg ctattgattg catccaatac atgtaatact gtactcataa tacattctct
     5881 cctccgtact atcttctttt aatgaaaata gatttgttac actttatcta ttctactact
     5941 tagagttgac tctaagtcaa tagcatttta aaaaaatttc agagaaagct tgttatataa
     6001 gtagatcttc gcacgaatca ggactcaaat aaaaaccttc tacagacaat taaaaagctt
     6061 tgtagaaggt ctcctaatta aggtctcatc aagaaatatg tttttttaat aggtgaagtt
     6121 cctgagcaat atgatacaaa tggatgtttg caccttttaa tgagattgga tagatctttg
     6181 tcttttcagt ttctaatcca ttttgatagc tagcagcatc gcacaaaacc actctttttt
     6241 tcgcacatag acattcatca ggcaattctt ctaatgaacg aaataaaata ggatttattt
     6301 cagcgatgcc ttcttccgca tacaaatgaa attgggttcg ataaaaaaat tgtttgactg
     6361 aactttctga aacaacaaaa atttctattt cctgtctccc cgcttgttca ctgttgatca
     6421 aagaaagact tttgacgaat tttcgaatga aattttcatt ccatcttagt tggatttgat
     6481 ttttcttttt ccactcttcc aatatttcta caatttttcc gtacagaagt tgttcttttt
     6541 ctgatagaag atagatcctt tccggtaaaa atacttgtcc atctgcccac aacgtacgga
     6601 caaattgtaa aaaggattgc ttgaaaataa aattttctgc atcgtcgatt tcagcgatac
     6661 taataatctg taaaatcaat tcattcagtg ataggtcatt gagaacaaaa tttttataaa
     6721 caacttcgaa atcttttctt aacagctcca tgttctcatt ggtataattt ctactgttga
     6781 acaaagaaaa aatataatag acatcttctt cttgtaagtc tagaattcct ttttgctctt
     6841 ccaatccttg acagactaat tgaaataacg gcttcgtttc tgcgcttttc ttatcttctt
     6901 ctttcagtcc aaggggaaat gcctttcttc cgattgcgat ctctattcct cgtatgataa
     6961 aggacttttc atcttccgaa tatcttcttt taaaaaaatg agtctctaca taatcaatca
     7021 actcgttaca agcttcttct acatacggat ttttattttc atagccttcc caattgacat
     7081 aacgtactaa agctagcaag atgttccgcg agtcaaattc tgggataatg atttctttat
     7141 ttttcaaata atcattttct ttaaaaaaat ccaatacgat ttttttgatt gtatatactt
     7201 tgctcaaaga aataaattca agatcagcca gttctgtact cgtgaatata ccattaaaat
     7261 aataacttaa aaactttaaa aaataagatt gaccgtacag aaactgtgtg agctcagaaa
     7321 gagcaatacc atgtttgaat acgagtttat acttatgctt ctctcttact tgttctttta
     7381 tacaatattc tagacactcg gctagacttt ctagatcgtg aaggatcgtt ggtgtagtca
     7441 cccttagaga aatagctgtt tgttcgatgt ctatttcttg gaattcaaat aattgttcac
     7501 acagatacac ttttctaaat atatttcgct cgatgtaatt ttccagcatt cctgatacac
     7561 gcctccagtt tttcttatga ttttatttaa tcatatctgt aaatcgtatt tttgtttcct
     7621 ttttatttag aaaagccctt tcaaaaacca ataaaaaaag aaactgggac aaaagtcaaa
     7681 caagctcaca atttcagcct cattgtaggt agctttgagc cgtttggact tttaccccag
     7741 cctctttcaa gaatggttat tcaaagatgt cagcgtctag ttctttgact acatctttca
     7801 acaattggat accagctgcg ctgttaccaa atggatctag tgccgggctg aagacaccta
     7861 tgccgtaacg atttggcaca gctgccatca acccgccgcc tacaccgctt tttgcaggta
     7921 caccaacatg gacggaaaac tctcctgatt catcatacaa accagcagtt gtcataattg
     7981 atttgacgat tgtagcactt tcttcagtaa tgatttgttt gccattccaa ggagcaattc
     8041 ctttatttgc cagcacagca gctaattttg ctatgcctgt agcggtgaca ttgagggagc
     8101 attgtctgaa ataagcatcg agtatctccg gtacgtctcc ttcgatcatt tgattacctt
     8161 tcatatagta agcaagagag cgattgatat cacctgttct tgattcagaa tgatagattt
     8221 cttcattcaa tgtgatatta ggatcatcac agatctcttt cataaatgac aagattcgct
     8281 caaatttttc atctgcatct ttaccggcaa tcaacgaggt tgttgcaatc gctcctgcat
     8341 taacaaaagg attcattgga tgtttgcgat gattgatttc catattcatg atagaattga
     8401 aggcaaaacc ggttggttcc gtgttgattg tttgaaagac tttttctatt ccgttgtctt
     8461 tgattgctaa taacaatacg ggaacttttg acatgctttc gatggcaaag cgcacatcgg
     8521 cgtctcctcc ctcaatcagt tgttttgtat ccaaatcata aatcgccata cctaattgtt
     8581 tgggatccac ttcagctaat gctggaatgt aatccgccac ttgtcctgtt tgataatagt
     8641 ggaaattttt tccaatcaat tgttctaatt cttttacttc catttgaata accagctttc
     8701 attttttatt tttctgcatg ctggaattta tgacggcatg cgtagatcgt aaatggaata
     8761 actaagacga taagataaga aacaatcaga atcgtttcat aaatcccatg ctgtccagct
     8821 gcgatactag caggtggaac aaatgagata caaaaagcaa aaagtgaagt caaaagacct
     8881 aaacaagcaa cgattacttt tacaactttt ccgcctggaa cctgataggc tctttcaaga
     8941 ctgtcatctt taaaaaccag cttcaaatag cccgcgaaaa acataaagta caacatcagg
     9001 tagattacaa cagtcaatga catcgctgca agaaatgaaa gattattccc cccgccgctt
     9061 aatgtaaaga cggctgccca gattgttgca acgatcccat ttgcgatcat caaatgagca
     9121 ggaacaccgt gtgtgttttc ttttgcaaaa tattcaggaa gtaatccttc ttctgctgct
     9181 acgtgaagtg cttttgaagg agaggttacc caagaagcaa tttctccgat gacacctaaa
     9241 caaacaagaa tcgcaagaat tttcgcaatc catgcaagat ttggatcaat gtgtcttaat
     9301 aggaattcta acgattgaat cacccctgta ttaagagaca aaccatgttg aggtactgta
     9361 gtagcgacgg tcaatccgcc aaaagtatct aaaatgattg ccagaataac caatacaaac
     9421 attgctaatg gataatcctt tttagggttt tccatttcat ttgcatgact ggcagatgtt
     9481 tctacgccca tatatgctaa aataaaggaa acaaaaacaa ccagcgtagg caatttcgaa
     9541 aaatcaggaa tcaatgtttt catagacagt atcgtttgta gtggagcgcc tgaagcgaca
     9601 taatggatcc ctaagccaaa caagactaat gctggcaaca agataccagc tacaaaaccg
     9661 atccgagcaa tcaatgaagt gtatttggtt ccttttaact gtgagagtaa aatagcccag
     9721 aaaataatca ataggattgc caatttcaga tatttattgt tttgtatagc accaataccc
     9781 gttgcatagg ataatgcacc agttaagaaa tacagcatgg tcaagtagcc aactgttatc
     9841 tcaaaccatt ggaaaaagat tgcggcaaaa ccgaatcgtt ttcccaatgt gcgactgacc
     9901 caagtataga caccgccttt ttcccatcct ttcaccgttg ccatctctgc tgcgcacaat
     9961 gccacaggga taaaccatag caaaccgcct aataacaaga aaaatactaa gctgaagcct
    10021 gaggtcgcga atgtgggata ttcatataat gaaacgacca ttgagatggt catagccaaa
    10081 aagccaaaga acgttaattt ttttgcagga attttcgttt tgctttgttg catgaatctt
    10141 cctcttttct atttgaattt gtttcaattg agtttgttgc caaagaagaa aaagtccaat
    10201 ccttcttctt gttcttcttt tgtagtagtg atcgttgtaa agtgcatcgc cgattttaaa
    10261 atcactgttg tcagcacata ggcatacacg gcagtaatga tgacaccaat cacttgaatc
    10321 cctaagaagt accagctgct tggattcgtt agtaaatggc tgttagatgc aaagatccct
    10381 atcagaatcg tacctgtgaa tccgcccatg ccatgaacac cccacacatc aagtgcatca
    10441 tcccattgtt tctttttacg aaaatcaaca gcagcatgac agacgatacc cgcaatgatc
    10501 ccaatcagca tggcactttt tgcttctaca taaccggcac aaggcgtaat ggctgccaat
    10561 ccagcaacgg atcctgttag tacgtcgaca aaatcaaagc catttccttt tacttttgca
    10621 atgatcagcc aagtaatcat cgcaaatgca agagcgatga atgttgaacc aaatgcattg
    10681 actgcttgtt catctgctcg aagtgcacca ccggaattaa aaccgaacca gccaaaccaa
    10741 agcagtcctg taccgatagc agctgccata agactgctat gctgacttct ttctcctaaa
    10801 ggaatctttc tttgaccaag tgtgaggata catgctaaag agccgaatcc agctgtcgtg
    10861 tggatgactg ctccaccagc gaaatctcta aatcccatct gatccaaaaa tccgccaccc
    10921 catatccagt ggcaaacagg gaaataaatc agcagattcc aaaaaataac aagtataata
    10981 tagcctttta aattcaatct ccctgcaaaa gctcctgtca tcaaaggaac ggtaatcacg
    11041 ataaacatca gttggtaaag aaagaacatt aaaaatggaa tcgttgcacc atgaaacatg
    11101 ttggggaagc ttgtaacatg tctcatcaaa aagaaatctg ccgggtttcc aataacaccg
    11161 ccgatatccc gtccaaaggc caatccgaat ccaccaaata cccacataat cgttgttatc
    11221 ccaacggaga gaaaagattg agacatgatc gttagtatat tattcttccg tgctaatccg
    11281 ccgtaaaaaa aagctagacc aggagtcatc aggcaaacca ttgctgtaca taaaatcatg
    11341 aatgctgtat ctcctgtatt gataggtaat gtattcattt ttttcgcctc ctttctacga
    11401 ttacgagtat aaaagaaggt gtagttgttt ttttcttctg tttttttgaa ttaatacttg
    11461 catttataaa agacagacaa ttaataaaag cttgatgtgt tgtgctcttt ttcgtttttc
    11521 acacaaataa agacaaaaaa taaacggtct tctttttata gaaaaccgtt tattttcgtc
    11581 tttttttatt tttaaagata ttttttcata aggaataaag aatacttcat ataaaatata
    11641 taaaaaagaa ccgcagcgag tgtcgctacg gttctcaaat ttgttcttca tttcaacata
    11701 agtttttctt caatttgctt ataatttacg tgcaaagaat gaaacgacta atactaaaat
    11761 gattgcaccg atcaatgatg gaatgatagc cattcctgca attgttggtc cccaagttcc
    11821 tagtaaagct tgtccaattg ctgaacctac taaaccagca atgatgtttg cgatgatccc
    11881 cattgatgat cctttattcg tgattgctcc tgcaattgct ccaatgatac cgccgacgat
    11941 taatgcccaa ataaatccca ttttaaattc ctcctaactg acttcttact tcttattgta
    12001 cacgtggttc gttattttct ttgacttttt gtgcaccttt gcctacagca gattttgctt
    12061 tatctgtttg gcgtgaagca aattgtcctg tggattttgc tgcttctgtt actttgtctt
    12121 gtactgtttc ttgatctttt tggtattctt cttgtgtttt gatgtctgca acattcacat
    12181 tcacttcgat aacatctaaa tgtgtcatat tgtttacttc agtactgatc aattctttga
    12241 tttggttgta aatatcttcg atgtctttac cgtattctac gatcacatct agatccacag
    12301 ctacttgttt cttacctact tctgtattga tacctgctgt tacgttatct gtgttgacta
    12361 atttttcagc catatttgag aaaaatccgc catctaatgt caatagacca tctacatctt
    12421 tcaatgcata accgatgatt ttttggatga ctttgtcttc ataagtcaat tcaccattca
    12481 tcatgcggtt ttctcctttt tcatttgcca aaactgatcc atttgttgta ttcatgtttt
    12541 tgttttccat ctttgtttcc tcctatgatt taaaaaattg atcaactatt cctgactgtt
    12601 tcaaataaaa accgattcct gcgccaattg ctgtcatgat tatgatcaac aatgtcttga
    12661 aaaatccaat cgttaaccat aaaacagcta gcagtagtcc aagaccccca aagataatag
    12721 ggagcttgta tgctgaaaat agttctttca ttatttggcc ctcctattcc actcgagagt
    12781 gcttgtagtc tcttgtatct tcttgttctt cataaccgct gtatgtgact gccactttga
    12841 ccgtttcttt tgttccgagt actttagtta cttcttcttc gatatcttgc atcaatacac
    12901 ctgttttccc tactaatgaa gaagttcgtt tcaagtctcc gttgacatgg atatggattc
    12961 gatttttcgt tgaacgtatc cgtactttag gtgaatgaat gaactcttct tcgtgtaaat
    13021 gggaacgtac caacccttcg atcgcttttt tgtcgattgt tagtttgcca tcttcccgtt
    13081 tcataacgaa agttcctctt gactttggat agaaaagaac gactagcaag atgataatag
    13141 caagaacggc aaatactaca gctgcccaga aaatatattg ttgcatgtag taattcgtta
    13201 aagtaaagaa gcgaaatcgt tccaattgtt caggcataat atacggcgca ttgatcaacg
    13261 caatcgctag aaatactgaa agtagcagta aactcatgat aacaccgatt atttttaacc
    13321 ccttattcat tttccacctc ctataaactt atttttaccc tgtaaataac tctaccgaca
    13381 atcacagtat agatagcgaa cagttagcat agcatcagta aataaagcgc taccaaacat
    13441 tgctggatca taacggcgtt ttattgccag tctgatactg aaataacaat tgcttactta
    13501 ttctctattt acttttgatt gctggctata cttaaaggat attattcaag attaaaaatt
    13561 gcaaagatta tgctcagtta ttattacaag attctattta tt
//
LOCUS       DAZKSU010000071        13147 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000071 DAZKSU010000000
VERSION     DAZKSU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.072"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..149)
                     /locus_tag="ACG8Z3_002159"
     tRNA            complement(77..149)
                     /locus_tag="ACG8Z3_002159"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="ACG8Z3_002160"
     CDS             530..1099
                     /locus_tag="ACG8Z3_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0824457.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="ACG8Z3_002161"
     CDS             1086..1946
                     /locus_tag="ACG8Z3_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0824458.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /gene="yycF"
                     /locus_tag="ACG8Z3_002162"
     CDS             2136..2840
                     /gene="yycF"
                     /locus_tag="ACG8Z3_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0824459.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="ACG8Z3_002163"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="ACG8Z3_002163"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0824460.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /gene="yycH"
                     /locus_tag="ACG8Z3_002164"
     CDS             4677..5993
                     /gene="yycH"
                     /locus_tag="ACG8Z3_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0824461.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="ACG8Z3_002165"
     CDS             5994..6863
                     /locus_tag="ACG8Z3_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0824462.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="ACG8Z3_002166"
     CDS             6918..7727
                     /locus_tag="ACG8Z3_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0824463.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="ACG8Z3_002167"
     CDS             complement(7830..8459)
                     /locus_tag="ACG8Z3_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0824464.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="ACG8Z3_002168"
     CDS             8629..9921
                     /locus_tag="ACG8Z3_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0824465.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="ACG8Z3_002169"
     CDS             complement(9997..10659)
                     /locus_tag="ACG8Z3_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0824466.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="ACG8Z3_002170"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="ACG8Z3_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0824467.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="ACG8Z3_002171"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="ACG8Z3_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0824468.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4370 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattca
//
LOCUS       DAZKSU010000072        13084 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000072 DAZKSU010000000
VERSION     DAZKSU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.073"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACG8Z3_002172"
     CDS             complement(78..2198)
                     /locus_tag="ACG8Z3_002172"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0824469.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACG8Z3_002173"
     CDS             2428..3606
                     /locus_tag="ACG8Z3_002173"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0824470.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8Z3_002174"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8Z3_002174"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0824471.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8Z3_002175"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8Z3_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0824472.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACG8Z3_002176"
     CDS             complement(4959..6212)
                     /locus_tag="ACG8Z3_002176"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0824473.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8Z3_002177"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8Z3_002177"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0824474.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACG8Z3_002178"
     CDS             7789..9135
                     /locus_tag="ACG8Z3_002178"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0824475.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACG8Z3_002179"
     CDS             complement(9443..9829)
                     /locus_tag="ACG8Z3_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0824476.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACG8Z3_002180"
     CDS             complement(10097..10246)
                     /locus_tag="ACG8Z3_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824477.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="ACG8Z3_002181"
     CDS             10708..10920
                     /locus_tag="ACG8Z3_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824478.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="ACG8Z3_002182"
     CDS             complement(11198..12997)
                     /locus_tag="ACG8Z3_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0824479.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaa
//
LOCUS       DAZKSU010000073        12927 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000073 DAZKSU010000000
VERSION     DAZKSU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.074"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG8Z3_002183"
     CDS             414..947
                     /locus_tag="ACG8Z3_002183"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0824480.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(1437..1961)
                     /locus_tag="ACG8Z3_002184"
     CDS             complement(1437..1961)
                     /locus_tag="ACG8Z3_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0824481.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8Z3_002185"
     CDS             complement(1954..2427)
                     /gene="tsaE"
                     /locus_tag="ACG8Z3_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0824482.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8Z3_002186"
     CDS             complement(2550..3530)
                     /gene="pta"
                     /locus_tag="ACG8Z3_002186"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0824483.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(3683..4363)
                     /locus_tag="ACG8Z3_002187"
     CDS             complement(3683..4363)
                     /locus_tag="ACG8Z3_002187"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0824484.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            4489..5358
                     /locus_tag="ACG8Z3_002188"
     CDS             4489..5358
                     /locus_tag="ACG8Z3_002188"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0824485.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            5384..5899
                     /locus_tag="ACG8Z3_002189"
     CDS             5384..5899
                     /locus_tag="ACG8Z3_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0824486.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(5946..6509)
                     /locus_tag="ACG8Z3_002190"
     CDS             complement(5946..6509)
                     /locus_tag="ACG8Z3_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0824487.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(6564..6743)
                     /locus_tag="ACG8Z3_002191"
     CDS             complement(6564..6743)
                     /locus_tag="ACG8Z3_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824488.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(6820..7329)
                     /locus_tag="ACG8Z3_002192"
     CDS             complement(6820..7329)
                     /locus_tag="ACG8Z3_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824489.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(7425..7805)
                     /locus_tag="ACG8Z3_002193"
     CDS             complement(7425..7805)
                     /locus_tag="ACG8Z3_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0824490.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            8135..8884
                     /gene="pnuC"
                     /locus_tag="ACG8Z3_002194"
     CDS             8135..8884
                     /gene="pnuC"
                     /locus_tag="ACG8Z3_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0824491.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(9068..10345)
                     /locus_tag="ACG8Z3_002195"
     CDS             complement(9068..10345)
                     /locus_tag="ACG8Z3_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824492.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="ACG8Z3_002196"
     CDS             complement(10424..11068)
                     /gene="pcp"
                     /locus_tag="ACG8Z3_002196"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0824493.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11267..12211)
                     /locus_tag="ACG8Z3_002197"
     CDS             complement(11267..12211)
                     /locus_tag="ACG8Z3_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0824494.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12213..12899)
                     /locus_tag="ACG8Z3_002198"
     CDS             complement(12213..12899)
                     /locus_tag="ACG8Z3_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0824495.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3726 a         2707 c         2295 g         4199 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaccaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
     1261 aacggcaaaa tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga
     1321 ttgaacaccg tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg
     1381 tgtgtttttc tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat
     1441 tcaatcaaat aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct
     1501 ctaggcatga ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca
     1561 ttacgaactt gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg
     1621 atgatttctt ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg
     1681 ataccgactt ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt
     1741 ccaataatcg tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata
     1801 taatccaact cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact
     1861 aaaaattctg tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct
     1921 ggcactgctt cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc
     1981 tagaattcgc tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg
     2041 cttttctaaa tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc
     2101 ttcttctaat agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc
     2161 taaccctaaa tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat
     2221 ccgaccttga ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat
     2281 ccctaatcct tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt
     2341 caatatcaga ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt
     2401 cattgttaat cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc
     2461 cagtatatcg cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct
     2521 gctgtttttg gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata
     2581 atcgataatt tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg
     2641 ttcaagcctt gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta
     2701 tagccgatat ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact
     2761 tctgaatctg gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat
     2821 tcgccgtcga tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact
     2881 ttgtctactt ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt
     2941 ggttcgatat cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct
     3001 tgcgcagttg gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt
     3061 ccacgtacca tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc
     3121 tgaagtgctg ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct
     3181 gcaatcccca tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct
     3241 gcttgttctt tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc
     3301 cacttatcgt aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca
     3361 tgcgctgcat ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct
     3421 gctttcaacc gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt
     3481 ttgatcccac ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct
     3541 ccagacgttt ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta
     3601 tctgtatcag tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact
     3661 ataacaggaa agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt
     3721 ctcctaacgc aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac
     3781 gacttgcaga taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga
     3841 tcattttgat tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc
     3901 gttcatttaa tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag
     3961 aatacgcttg cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag
     4021 cccatgacac tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt
     4081 tatagatatt tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc
     4141 catgagcttg attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt
     4201 cataaggagt caattccaat gcttcataga tatgatacat atctggatgg atcttttggg
     4261 acgcatattc tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg
     4321 aggacaaaac ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat
     4381 aaaaagaata actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga
     4441 ttaatatggt aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta
     4501 attgcatcag atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat
     4561 gccgaggcga tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga
     4621 aactatcaag aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact
     4681 ttgaacgggg cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca
     4741 aataatcaag caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta
     4801 gcaaccaacg atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc
     4861 atggtggcta cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct
     4921 aagctggagt tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag
     4981 aatctggaag ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca
     5041 gttggaaagc agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat
     5101 atcgaagtga accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag
     5161 cgtgggattt ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg
     5221 ctgcaagtag caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat
     5281 gccaaatata caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg
     5341 attgcagaag acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat
     5401 tcaggaaaag cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc
     5461 cctgtttttg atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat
     5521 gaatggcgat ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga
     5581 gctgtataaa gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga
     5641 cttttattat attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata
     5701 tcaaatccat catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg
     5761 agattactat gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc
     5821 tgcggtttta gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta
     5881 ccgacttaac ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca
     5941 tctttttaga tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg
     6001 acaactttga agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca
     6061 cttagaccga tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat
     6121 cgcgaaccga tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat
     6181 tcgttgggaa acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc
     6241 tctaagatag gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa
     6301 gcaactcctg caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga
     6361 taatttggat cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc
     6421 ttcaaaaatg gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga
     6481 gcatcttctt ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg
     6541 cagaaaaaga aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa
     6601 taaccatgag taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt
     6661 tctttatcat tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa
     6721 ttagggcatt tcattttttc catgttatct caacttcctt tcatctttac tctcttagct
     6781 tactcttata ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg
     6841 acggtcctgt tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag
     6901 aagtgtccac tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc
     6961 tgcttgcggt tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt
     7021 ttccgtgtca tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc
     7081 ttcacaaaaa aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac
     7141 tgcaaaatga atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc
     7201 ttttttcaat gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt
     7261 gagcagtccc gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat
     7321 ttttttcatt cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat
     7381 gaaaaatcga atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct
     7441 aatggcaatc cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct
     7501 ctaatcggtt ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg
     7561 tctactttga aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc
     7621 gcattagcaa ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag
     7681 tcatctcgtt ctttgcggta attttctctg attacttcaa atcctaattt attaacataa
     7741 aaatcacgtg atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg
     7801 ttcatatgag cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat
     7861 tgtaaatgtg tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa
     7921 aaaaacagaa agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt
     7981 aagaaattat attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat
     8041 tgaaagacta gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac
     8101 acactacatt tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc
     8161 tcatcagcta aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg
     8221 ccaggtcatg actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg
     8281 taccacgctt ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat
     8341 tttaggtatt ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt
     8401 aagtatcggt gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa
     8461 agaatggaat cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat
     8521 cgcaatcatc agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac
     8581 agatgatcct cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat
     8641 catcagtttc ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca
     8701 aatggtcctt tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa
     8761 cagctctgtt tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca
     8821 aaaagaacgt gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc
     8881 ataaaaaaac tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     8941 tataaacggc ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
     9001 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     9061 ctatttttta tagtaattcc ttcagatact ttcttgcctc atcattgaaa cgcagtgatt
     9121 cttctaactt ctgataatat tccaacgctt gtttctgagc ttcagtaaaa cgataatgtt
     9181 ttcctttcca tttgtcgatt gtctcttgca catttccaga ttggatatct ttcactacta
     9241 gttgccattt ttcttttgag actgtacggt ctttcttatc agcagattgt atagcttctc
     9301 gttctttacg caatactttt aaaaaaccat ttctgactat ccgaggaatg ctgtcgaaga
     9361 acccttctgt aaaaccatag cgacaatatt gcttccaaat aaatagatag atttttggtt
     9421 ggatgaatcg ttggtttttc gcaaaatatt ccatctgagc agtgacttct ggtgttcgtt
     9481 gacgttttgt ctttctcaac acggaaataa atgtttcaaa ataagcatta ggatgtactg
     9541 gcacttcttc atatacgatg gattgccaat ctttacgtaa aaatgcgcga tagttgatca
     9601 agaattcttc tacatccttt actaattctt ctgcaaagtc gtcaaatagt tcttttttta
     9661 tctgtcgctt ttttgactgc ccattgatgt gttcacttat tttcttgatt tcattttcca
     9721 atgcttgata atccgctatg taaatcggca tttctacttg gcgaaattct gcgagccgct
     9781 gagctaattt tatatccggt aaatatggct gtttcatccg acgattttgg tacaagacaa
     9841 aacagtattt ttgccatagt ccttcaggaa aatcgatatt cttctgctta agccataata
     9901 attcgtctaa ggccagatca acttttgtca ttccctgatg gatcgaagct gccactgtcg
     9961 gagagacatg caggtgatcg gcaaagtggt tgatgcctag ttttaggatc tttccggttt
    10021 ttggcgattt aacgatgtat tgcactttta attctctacc acactcacaa tacaattttt
    10081 gctggcttcg ttttgttcga aaaaacggat tgatcttgaa gtcagaaaac acccaatctg
    10141 ttgcagctaa ataatgttct tccataaaac gagagctgat tagatatcgg cggtgcttat
    10201 cgactagttc ccgctgtttt ttcgttaatt ttgcatagat ctcccaacgt tgactgcttg
    10261 tgtattgttt agaaattttg atgggatagc ttttgctgat ttcttttgga aaccattctt
    10321 caaagacgga ttgccattct tccacggctg tttctttcct ttctaattac aaaaagctgg
    10381 ctactaacat aacgttagca accagccagt gtttcttgct aaattagtga gttgttccgg
    10441 catttccgga aatatcttct ttattttcta ctgctgcttc aactgcataa gtcaaagaag
    10501 aagcaatgtc ttctaaagac atagaaggcg tacccactgg acgatcgatc acttgatcgg
    10561 gtgagaatgg cacgtggatg aacccacctc gaattgccgg gaattgtgtt tcgatcaaat
    10621 gaagcaaacg atacatgata tcattgcaga caaatgttcc tgctgtataa gagatgtacg
    10681 ctggtagacc atgatcttgt acattttttg tcattgcttt gattggcaat gaggtaaagt
    10741 aagcggctgg accgttttct tctaaagccg tatcgaatgg ctgatttcct tcattatcag
    10801 ggattcttgc ttctgctaga ttgattgcga cacgttcgaa cgagactgct gaccgcccac
    10861 ctgcttgtcc gacacagatc acgacatctg gttggtgctg ttggatggct tctttgacta
    10921 cttgactgct tttttcgaat acggtcggta cttccacttt gatgatttca gcaccgctga
    10981 tttcatctgg catttttttt actgcttcat aggctggatt cactttgtcc ccaccaaatg
    11041 gatcaaaacc tgttactagt actttcatct gttgttcctc cgtccgattg atttaacata
    11101 tggttatctt ttatcatacc attttccttg aacagcgtaa ataaaggaaa caatagttta
    11161 aataacataa cagatcaaac ttaatatatt cttataaaaa aatagtgttc cactacaatg
    11221 ttagaagagg ttgcgacaat tattctgtca caacccctct cttctattat ttaaatagaa
    11281 tcatataagc aatttggaaa actagcataa ataacgcaat aaacaactgg tttttgatca
    11341 ctccgtattt gtctttcatc tccagcatcg ctacaggcac gatattgaag ttagcagcca
    11401 tcggtgtcag cagggtccca caaaaaccgc aagtcaatgc aaccattcct attaacgctg
    11461 gattcgctcc atgactaaat acgaatggtg caccgatccc aaccgtcatc acggtgatgg
    11521 ccgcaaatgc attccccatg attaccgtaa atatcaccat gccaagagcg taaatgatga
    11581 tgccgatgtt cacatttcct tctggaacga tcgtacctac tgcattggaa atcactgtcc
    11641 caactccagc gcttgtaaat accgcaccta gagaagctaa aagcattggc aacatgctta
    11701 acggccctac tgtgcccagc atatctgctg catcatctaa aaatgtactt ggtcgattat
    11761 ctcgagaaaa gaacatcaag attagcatgg ctgcaaaaac accgacgccc acgccgacta
    11821 aagcacctaa attggtaaat agagcaaaca agatcgcaaa gacaccgata ctcaaagctg
    11881 gaataaaaat tttcattcct agtttgtctg acattttttc catatattct tttgatggaa
    11941 gccggctttc tcctttgctg actttcttag caattgctgg tatggtcatc gcaaagatca
    12001 agccaccgtt cacgattgcc ggaagccatc ttcctcctgc aataactacc cctaagaccg
    12061 tccagaaaaa ggctgaccca taacgatgag gattggtttt atctgttaaa tttcttactc
    12121 cagtgtaaat caatacgagt cccatcacga tataaaggat ctccagtaat ttatcgccta
    12181 ataaaatatc aggatttgta aaaaagtcca tcttattgcc cctttcctgg ataatatttc
    12241 ttcgctaatt tcttttcttt aagatagaag tagacacttg ccacaactaa agccgtgata
    12301 gccactggaa cttccactaa agcaagctgt cccaatgtta cgtcatatcc tagatctttc
    12361 agtgttgatt ggactagcaa cgcccctgct cctccgatga atagtacttg gccaaagaac
    12421 cagcagacat tttccatagc tgaagccata ccttttagtt cttcttcatg cttaggatgg
    12481 acttctaacc cttttgattc gatggtcccc aatgccatcg gtaaaatgat tggacgaaca
    12541 aatccagcaa ctccaccaaa actcacgtta aaagccgcaa agatcattcg aaagatccca
    12601 taaatatcta caatcatacc agcagatact ttcttgaatc gcttgatcaa ggaagccgct
    12661 gattctttca gaccatttct ttcaagcgtt cctgttgcca acataatgat gataaaaatc
    12721 gccatcccgc gattgtttac aaaacttgta cctaaaagtt ctaacattcc ttgaatgcca
    12781 agaccgccag tcaaagctgt aacaactaaa gcaaccatta cgatcaatat cgagtctaat
    12841 ttaaatgcaa acccgagaat aacaatcaag acgcctaaaa ggctaagata attttccata
    12901 tattttcctc cataaaatga caatatt
//
LOCUS       DAZKSU010000074        12889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000074 DAZKSU010000000
VERSION     DAZKSU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.075"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..435
                     /locus_tag="ACG8Z3_002199"
     CDS             <1..435
                     /locus_tag="ACG8Z3_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0824496.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="ACG8Z3_002200"
     tRNA            complement(759..842)
                     /locus_tag="ACG8Z3_002200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="ACG8Z3_002201"
     CDS             1370..1870
                     /locus_tag="ACG8Z3_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0824497.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="ACG8Z3_002202"
     CDS             complement(2060..2404)
                     /locus_tag="ACG8Z3_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0824498.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="ACG8Z3_002203"
     CDS             complement(2558..3478)
                     /locus_tag="ACG8Z3_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0824499.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="ACG8Z3_002204"
     CDS             3665..4156
                     /locus_tag="ACG8Z3_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0824500.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="ACG8Z3_002205"
     CDS             complement(4180..6555)
                     /locus_tag="ACG8Z3_002205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0824501.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="ACG8Z3_002206"
     CDS             6881..7507
                     /locus_tag="ACG8Z3_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0824502.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="ACG8Z3_002207"
     CDS             7530..8234
                     /locus_tag="ACG8Z3_002207"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0824503.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="ACG8Z3_002208"
     CDS             8426..9259
                     /locus_tag="ACG8Z3_002208"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0824504.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="ACG8Z3_002209"
     CDS             9456..11384
                     /locus_tag="ACG8Z3_002209"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0824505.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="ACG8Z3_002210"
     CDS             11522..11977
                     /locus_tag="ACG8Z3_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0824506.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12057..12185
                     /locus_tag="ACG8Z3_002211"
     CDS             12057..12185
                     /locus_tag="ACG8Z3_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824507.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            12447..12716
                     /locus_tag="ACG8Z3_002212"
     CDS             12447..12716
                     /locus_tag="ACG8Z3_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824508.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
BASE COUNT         3951 a         2427 c         2341 g         4170 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatgggt gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaa
//
LOCUS       DAZKSU010000075        12796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000075 DAZKSU010000000
VERSION     DAZKSU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.076"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACG8Z3_002213"
     CDS             4..2376
                     /locus_tag="ACG8Z3_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0824509.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNDQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQVPEKEQKQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGHTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQD
                     EKNVVETAEQKNIVLVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDTQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACG8Z3_002214"
     CDS             2437..3897
                     /locus_tag="ACG8Z3_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0824510.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVQDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEMKVEPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGKQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACG8Z3_002215"
                     /pseudo
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACG8Z3_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            6128..6277
                     /locus_tag="ACG8Z3_002216"
     CDS             6128..6277
                     /locus_tag="ACG8Z3_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824511.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6954
                     /locus_tag="ACG8Z3_002217"
     CDS             6358..6954
                     /locus_tag="ACG8Z3_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0824512.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDTERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6967..7866
                     /locus_tag="ACG8Z3_002218"
     CDS             6967..7866
                     /locus_tag="ACG8Z3_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0824513.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKEQKLAYQRHQQMNREMER"
     gene            7869..8353
                     /gene="ssb"
                     /locus_tag="ACG8Z3_002219"
                     /pseudo
     CDS             7869..8353
                     /gene="ssb"
                     /locus_tag="ACG8Z3_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            8375..8632
                     /locus_tag="ACG8Z3_002220"
     CDS             8375..8632
                     /locus_tag="ACG8Z3_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824514.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8791..8925)
                     /locus_tag="ACG8Z3_002221"
     CDS             complement(8791..8925)
                     /locus_tag="ACG8Z3_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFQ0824515.1"
                     /translation="MLKSIKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK
                     "
     gene            9068..9328
                     /locus_tag="ACG8Z3_002222"
     CDS             9068..9328
                     /locus_tag="ACG8Z3_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824516.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9777..9983
                     /locus_tag="ACG8Z3_002223"
     CDS             9777..9983
                     /locus_tag="ACG8Z3_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0824517.1"
                     /translation="MRKKFDYLGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIEEKGKKR"
     gene            9983..10234
                     /locus_tag="ACG8Z3_002224"
     CDS             9983..10234
                     /locus_tag="ACG8Z3_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824518.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGCELHYQENLETYENEYWLTKLEN"
     gene            10250..10687
                     /locus_tag="ACG8Z3_002225"
     CDS             10250..10687
                     /locus_tag="ACG8Z3_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824519.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCFEIGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(10798..11025)
                     /locus_tag="ACG8Z3_002226"
     CDS             complement(10798..11025)
                     /locus_tag="ACG8Z3_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824520.1"
                     /translation="MKQNTSITKNTARFNCTAALSRISISPTNSTNNKGIIFFKWITF
                     FLLSYRALPVYFAPPKRRGRLVRNKNHSLSY"
     gene            11123..11311
                     /locus_tag="ACG8Z3_002227"
     CDS             11123..11311
                     /locus_tag="ACG8Z3_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824521.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IACIGQYLYCIRGRKLYA"
     gene            11429..11635
                     /locus_tag="ACG8Z3_002228"
     CDS             11429..11635
                     /locus_tag="ACG8Z3_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824522.1"
                     /translation="MKYNLLDHKRMWVEKLEEDLEQLELLGHNKTSTVYKEGLKRTKK
                     AQKELATLESRVQKKEVDLTHTYL"
     gene            11645..11878
                     /locus_tag="ACG8Z3_002229"
     CDS             11645..11878
                     /locus_tag="ACG8Z3_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFQ0824523.1"
                     /translation="MADKRKEKIQKLLALAADVNDEESVSALAKAQQLMMDYDLTEDE
                     IFNYHEQQRIEAVETHVIYRGRPQKWLYRLFRN"
     gene            12200..12403
                     /locus_tag="ACG8Z3_002230"
     CDS             12200..12403
                     /locus_tag="ACG8Z3_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFQ0824524.1"
                     /translation="MILGIVLLSVSTLGITVDAATYYGNGVYCNTQKCWVDWSRARSE
                     IVDRGVKAYVNGFTKVLGGVGGR"
     gene            12393..12584
                     /locus_tag="ACG8Z3_002231"
                     /pseudo
     CDS             12393..12584
                     /locus_tag="ACG8Z3_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12655..>12796
                     /locus_tag="ACG8Z3_002232"
     CDS             12655..>12796
                     /locus_tag="ACG8Z3_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0824525.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
BASE COUNT         4743 a         2111 c         2688 g         3254 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgat caaatcgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaacgggc attcttggtt taggcaacaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggtggc
      301 ctggatatga cgatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc tgccacgatc cgaaaatacc aacgaaaatt ggaaacatta
      421 gaacagctga aagaaaaatc agagaaagga aaagaaaata ttcttcctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaagaatc caactatcta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactcaat gaaaacgggg gatttgagga atctaaaaaa
      601 taccaagcca cgtcggaaga agaaaaggca gctgttcgtg aattacttga agaagctaca
      661 cctactgatt ctgtgaagga gaaagaaacc gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag caaaagggtt gaaaagttat ggtggggaga tcgtaacact agaattgatc
      781 gatcaactca aaacactgga taaccaatac gccgcagaag caggctatta taaattctat
      841 tttgaagaag tgaagaatgg agaagtagta gatcagatac gaatggatct aggggatggc
      901 gtcaatagta atcaccccat ctatttagag ttagctagat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaaacagt caaaaatgaa cctatttcaa aagtacaaaa tgagattaaa
     1021 gagacaaaaa caaaacaaaa gggttatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatcac tttcccaaca ggcgcttgat gcggtgaaaa agcatactca ttcacctgaa
     1141 gacgtcaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgatcg aggaacaatt tccaggcgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaggc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat caaacagggc atacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagccac aagtattcac
     1741 gaattagccc atgctagaat gcacaaattt tcagaattag atactgcaac aaaagaacta
     1801 caggcagaaa tgacctccta tattgtgtgc aagcattttg ggatggacac ctcggagaaa
     1861 gctattcctt atattgcgac atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agtcgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccagcc agcagaagtg
     2041 aagcaggatg agaaaaatgt tgtagaaaca gcagaacaaa agaatattgt gctcgttggc
     2101 tcactttgga tcgacaagaa agacgggaaa gtcctggaag aagacacggg ccgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat acgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gattcaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatttg
     2821 aaggaagaac gaaaactgcc cgattggttg attgattatg cagaaaaaga aggattgatc
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcaagacga tcgtttggat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacat tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggatttaac ctaaagatcg gtgaccctaa acagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccattga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtt gcaaaagaaa tgaaagttga acctgaagca
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aaggttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatccacca acaagagatc
     3841 agaaagcaaa aacgtgcgat gttgtttcaa caaggcaaac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccaccc gaaaagacgg gtattttgaa atccaagata gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcagtg attccagcaa ttacgagacg cagaagagac
     4381 ctatcccaaa tttgaagaag cacaagcacg ccagatcgct gattggttga tcggcatgaa
     4441 cggcagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcag ctgcaggaaa agattagaaa
     4561 cttccaaaaa gagccttatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atacaagggt tgtatgaagg
     4861 gaaatatcta agctacccac gaacagatac cccttatatt acagaagggg agtatgcgta
     4921 tctgttggat cacttagacg aatacaaaca ctttttaaag gccgaagcca ttcctactcc
     4981 tattcataca ccgaatagtc gatatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtca tgacggcagc ggcttttgaa caattaagtc cactgcagca
     5101 ggccatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ttaaaggcaa tcggaaaggt
     5221 gccgctagat ctaggttgga agaagttatt tggaaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtgg atctacaagt
     5341 tcttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcaat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agctcgtggt gacgaataaa gggattttgc tttgccaagc
     5581 cgtggaaaaa gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatc ggtgaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt
     5701 tgtctctcac ttattggaag tcgtaccagg gcaaatccaa tccactgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttgct ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatc attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcattt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgccgc actgcaaaat aatggagggt
     6121 taatcttatg cgtaatacca cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga ctgcagcaat tgaagaacca
     6241 gcagaggatt caaaaagaaa tcgaattaga tcgataaaat cttcctaact attgtatacg
     6301 tataaactta tgttatactg tttttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtatacaa gctgctagaa gaatttaatg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg aggggagctg
     6481 gatcgagagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcgcaaaaaa aatatgaacg cggcattatt tcacatatta ccgcgttaga tttgtatgat
     6601 ttaacagata tgatccctag acagatcgat ctgactgtac cttacggtta tcacgtttct
     6661 gaaaaaggat tgaaggaatt cgcagttgag ctacactata caaaagcgga atggtatgaa
     6721 ttaggtaaga ttgaaataaa aagtcgctat gggaatccta ttatagctta tgatacagaa
     6781 agaactttat gtgacatttg gaatccctgg tataccgttg aggatgaaat caaagtaaaa
     6841 gcaatcaaaa actatatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttac caacagacaa aacgatgcgt tcttatatta tggctttaaa ttaaaggaga
     6961 agatacatga cacctacgca gttaaaagca aaagtgaaga atatttctag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttgattgg ctcaaagtat
     7141 ggcattgaaa atcgcacaac caaagatatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat taacaacggt tctaaacgaa attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaacg agagaagccg attactatcc aggatttagc
     7321 ttacgtgtgc tggcacattt ggaaaacatg cggccagatt ttaaggtaga tgtgactaca
     7381 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaggatcga
     7441 acgattcctt tggaatccta tcctacagaa caaattattg ctgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaga agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatatac tatgaaaagg atatggatgt actgaaaaat
     7681 agtcacgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcatcg
     7741 atcacgtacg atgagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcaaaaact agcttatcaa agacaccaac agatgaatag agaaatggag
     7861 agataaagat gattaataat gtgacattag taggacgctt aaccaaagat cctgatttac
     7921 gttataccgc aagtggtact gcagtagcga cttttaccct agccgtcaac cgaaatttca
     7981 cgaatcaaaa cggcaatcga gaagccgatt ttattaactg cgtgatttgg cgcaaaccag
     8041 cagaaacaat ggcgacatta gctaaaaaag gtagcttgat tggtgtagtt gggcggattc
     8101 aaacgcggac ttacgacaac cagcaaggac aacgtgtata tgtgactgaa gttgtagcag
     8161 ataactttca actattagaa aataaagcag cgacagagag ccgtgcgcac gctgatcaat
     8221 cgtccaccag tccaagcacc gccacctttg aacaacggat acagcgacgc ctaacaacaa
     8281 tggattgaac gcttcacaga atccatttgg tggacaatca atcgatatta gcgatgatga
     8341 tctaccgttt tagtttgaga aagaaggagt aagcatggca aaaaaaatta gtctgacgat
     8401 ggatctattg atacccgtaa ttgcatttat agacgggatt tctttgggcg tcactgaaaa
     8461 tcctttggta aatagaattg cgaaaggatc actatttctt gtattcgtgt atcttgtctg
     8521 ttcttatctt ttaaaaagag caggaaagga taattccttt tggcgattga ctaaagtcac
     8581 agtagcagcg acagctgtta tacttacggc tattctaccg ctttatatgt agagttaacg
     8641 ggaaatcgtt gaacttcttg cgaaatattg ctataatgaa catgaaaaag aggagttgcg
     8701 ctaacaaccc ctcattaaca gcaccgatta aagaacggtg gcagaatatt agttatttgg
     8761 aaataaccgc tgacctgcta aagtagacgg ttatttcttt ttgtcatttt taagcatatt
     8821 tacaatcaaa ctaatcaaag caatggtaaa cataccaaaa ccaagaatgg tttgaattgt
     8881 ctcatatgcg gacaaaaggc ttactccttt tatagatttt agcacttaca ttcataagca
     8941 ccaccacctt tccagaagtc gccaccgtct tcaaacttta ctgtcttgac tattatagca
     9001 tatctaaaat cgaataaaaa ttctcaattt taactgtttt tgctataatt ttagcgaggt
     9061 gatcgtgatg aacaaatatg ttggtttgct tgataaaatc cgagtaataa aaacacaacc
     9121 attattggtt cgctttactt tacaaacaat tcatgaatcg attaattgtg tagttgcaga
     9181 tattgaaatt atcgataaat tgttaattat ggatgatgga aaatacaata ttgccgtcac
     9241 aggtcatttc aataaacgga atcaattagt cattgaatcg atgcaaatta gaaaccctga
     9301 tcattttacg cgatcaatgg gcatataaat aaaagaagag ctgcaatcct aatgtaggac
     9361 tgcagctctt tttgttttac atagaaacag gaagaaaggg aaaggcctgt ttaggaaggc
     9421 acaagtgcct tctcaattga aaaacctttc tctcactcca accgccaccg ccgttcgaga
     9481 agaaaggaag tgaacgttgc acgttcacta agataagggg tcttgctttg taagacaaaa
     9541 taggaaaagc ttcctctcac caaaaggttc gttcctgttc gctatctctt tattgatttg
     9601 ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag ggtaaatggc
     9661 acgctgcgcg cccttgtcct tgtcgctttt cgttgattca ttctggccaa caaatcaata
     9721 aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa tcaatcatgc
     9781 gaaagaaatt tgattatttg ggtgttccct tcagcgaatt attcccaaac tatcatgcac
     9841 cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc tattgtttgt
     9901 atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattat attttgattg
     9961 aggagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgactg taaaagaagt
    10021 cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa gtagcagaag ctttacttat
    10081 tccgcggtac tggacgatag aaaccccttt agtagaggat gcaccgacta tgctatttga
    10141 agacctagtg aagattgcag gatgtgaact gcattatcaa gaaaacttgg aaacttatga
    10201 aaacgaatat tggttaacca aattagagaa ctaaaaagga gaaaaaaata tggctcaaaa
    10261 ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa tggaggatat
    10321 gacgttccta tacaacgaaa cacgtgttcc aaaaaaacat tataaagaaa aattgtcggt
    10381 agctgtggaa gaaatgattg aatcaggtgt cgaaatgaat cttattgcga cgtactatcg
    10441 aacactggaa gaactgaaaa agcaaaatgc aaaatggttt ttccaagcct tactatgctt
    10501 tgaaataggt gtcaaaccaa gcactattaa accttctgaa taccaggctt tagaactaac
    10561 gtatgcgaaa tttatcgaaa cgaaaaaagc caagactgtt tcaagtgaat ggttagatta
    10621 ttttgaaaat atcaataagt atggcgcata ttacaccatg aaaaaggaag acaacgaaaa
    10681 tgaataaggt gatttatcaa ctaacaacgg attccatatc aacgatttga aagaaaaaaa
    10741 ggtcaataat catttatacc gaaaagtgag aatgtgtgga ataaggacaa aaaaagccta
    10801 gtaactaagg gaatggttct tatttcgcac caaccgcccc cgccgcttag gtggtgcgaa
    10861 ataaacgggc aaagcccgat aacttaagag aaagaaggtg atccatttga aaaagattat
    10921 tcccttattg ttagtactgt ttgttggcga aatacttatg cgagatagtg cagctgtaca
    10981 gttaaacctc gctgtatttt tggtaatact ggtgttttgt ttgatctttt ggcggtttgt
    11041 attagtcctc gtgagtctac tattcaagat gattgcagcc atatttttca tggggttatt
    11101 tcgatgaaag gagagagtac caatgccaat tgcaatagga aataaacggt tacccgtgac
    11161 attagatgaa aagagacaaa aagaattgca gcaactaaag cagaagtacg gcaaaagtga
    11221 atccaggatt atgtgtattg cgttagattt attgattgcc tgtattggac aatatctcta
    11281 ttgtattcga ggaagaaagc tttacgcttg aatgcgataa gttaacccga tatatcgatc
    11341 caaaattcta tgaggataaa aacctggcat gggaactggc tttatagaca gagtgctagt
    11401 gcaagtaaag gaagaaagga agatcaccat gaaatacaat ttattagacc ataagcgaat
    11461 gtgggtagag aaattagagg aagacttaga acagttggaa ttgttaggtc ataataagac
    11521 ttcaacggtg tataaagaag gactaaaacg aacgaaaaaa gcacaaaaag aactagcaac
    11581 actggaaagt agggttcaga aaaaagaagt agacttaaca cacacttacc tttaggagta
    11641 gaaaatggca gacaagagaa aagaaaagat tcaaaaatta ctggcgcttg cggcggatgt
    11701 aaatgatgag gaatcagtga gcgcattagc caaagcccag caattgatga tggactatga
    11761 tcttactgaa gatgagattt tcaattatca tgagcagcaa cggatagaag cagtagaaac
    11821 gcacgtgatc tatcgtggac gtccgcaaaa atggctgtat cgtttattta gaaattaaga
    11881 taaaaaaggc tagttaacag gtttgttgac tggccttttt tgatatgttt gtacatgttt
    11941 tattgtgttt taatttttct cttttttata gcaagctcct tttataataa atattccgaa
    12001 aagtgaagac cattttttat ttattaagac tattattatt agtgagataa aatttgattg
    12061 atcttacaga atattggtga cgattatgac actaaagata ctatttatga aaattttgca
    12121 aaaatgttct gaaaattaca tataattgtt ttataaaaaa gggaggcaat tatatgaaga
    12181 aaaaatttgt tagtattttt tgattttagg aattgtttta ttgagtgtat ctactttagg
    12241 aattacagta gatgctgcaa cttattatgg aaatggtgtt tattgtaata ctcaaaaatg
    12301 ttgggtagat tggagtagag ctcgctctga gattgttgat agaggagtaa aagcttacgt
    12361 taatggtttt acaaaagtgc taggtggagt cggtggaaga taaaaaagaa aaactttttg
    12421 acttaattgc gaatgcatat aattcaaagt catataataa tgtagaagta aaagaaggat
    12481 ttcttcaaga tgctaagaaa atacgagagg ataaaccata tcctgaaatt attatatcag
    12541 tgtacaaaca tttatctaaa ttaaaagctg aggaacgcat ggataggcta caattaacta
    12601 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
    12661 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
    12721 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
    12781 aaatgggtaa agcaat
//
LOCUS       DAZKSU010000076        12335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000076 DAZKSU010000000
VERSION     DAZKSU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.077"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..164
                     /locus_tag="ACG8Z3_002233"
     CDS             42..164
                     /locus_tag="ACG8Z3_002233"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824526.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            137..268
                     /locus_tag="ACG8Z3_002234"
     CDS             137..268
                     /locus_tag="ACG8Z3_002234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824527.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
     gene            807..1007
                     /locus_tag="ACG8Z3_002235"
                     /pseudo
     CDS             807..1007
                     /locus_tag="ACG8Z3_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1368..1673
                     /locus_tag="ACG8Z3_002236"
     CDS             1368..1673
                     /locus_tag="ACG8Z3_002236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824528.1"
                     /translation="MVHYRKILELYFSGISQRTISLTVGSSRHTIREVTSRADRKGVT
                     ELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKNMTLKLLHREYGVHGT"
     gene            complement(1719..2324)
                     /locus_tag="ACG8Z3_002237"
     CDS             complement(1719..2324)
                     /locus_tag="ACG8Z3_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0824529.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2506..2841
                     /locus_tag="ACG8Z3_002238"
                     /pseudo
     CDS             2506..2841
                     /locus_tag="ACG8Z3_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3006..3221
                     /locus_tag="ACG8Z3_002239"
                     /pseudo
     CDS             3006..3221
                     /locus_tag="ACG8Z3_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3359..4330
                     /locus_tag="ACG8Z3_002240"
     CDS             3359..4330
                     /locus_tag="ACG8Z3_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0824530.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4470..5783
                     /locus_tag="ACG8Z3_002241"
     CDS             4470..5783
                     /locus_tag="ACG8Z3_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0824531.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5826..6692
                     /locus_tag="ACG8Z3_002242"
     CDS             5826..6692
                     /locus_tag="ACG8Z3_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0824532.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6689..7537
                     /locus_tag="ACG8Z3_002243"
     CDS             6689..7537
                     /locus_tag="ACG8Z3_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0824533.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7551..9023
                     /locus_tag="ACG8Z3_002244"
     CDS             7551..9023
                     /locus_tag="ACG8Z3_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0824534.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9040..9912
                     /gene="scrK"
                     /locus_tag="ACG8Z3_002245"
     CDS             9040..9912
                     /gene="scrK"
                     /locus_tag="ACG8Z3_002245"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0824535.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(10146..11527)
                     /locus_tag="ACG8Z3_002246"
                     /pseudo
     CDS             complement(10146..11527)
                     /locus_tag="ACG8Z3_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11989..12312
                     /locus_tag="ACG8Z3_002247"
     CDS             11989..12312
                     /locus_tag="ACG8Z3_002247"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0824536.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
BASE COUNT         3845 a         2093 c         2480 g         3917 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg tcgatctaaa
     2281 ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac tccttttaaa
     2341 aaatgatata atttacatgt aaaatttact tgtagtttta ctgttatcat agcattttta
     2401 aaaataaatt ttaagtctac ttataattta ctttggaggt tttgaatgaa tgaaattaag
     2461 cgcaataaaa attcttttgg tgtttattta ggagagccta gctcggaaca gttaaataaa
     2521 ggtgaagctc gtcatgctct atgtcgaaat atattttatg gtagaaaagg caaactttat
     2581 caaacttatt acgacggtat ggaggaacag ctcaacgcat taggattagt aactaatgct
     2641 atcatatatt ggaattcact atatttagaa aaagtctttg aacaaattaa aacggaaggg
     2701 ttcgactgtt ccgaagaagt gttacgcaag caatttccat taattagtga tcacattaat
     2761 tttgttggca aatatagttt ccgctatgaa aaagaattat cgaatggaat actacgtccg
     2821 ttaaatatag agaattattg agaatgttcg tcttttggtt cctaccgtgc attttcgcac
     2881 ggatgttcca gatactattt ttgaatgtat aaaattgaaa acacaagaat actaataaat
     2941 caacgattct tgtgttttta atttttcact accgtgcatt ttcgtttcta tgttccatgt
     3001 accccaatct tgaaaaatta attaaagact tagataaagc cattgacgac ttagtcgtgg
     3061 tgctaccgga gtatcaatgt ttaaccagta tcccaggtat tggtcctgtt taggctgctg
     3121 gaatactcgc tgaaattggt ccaattgaac gtttttctga tgaaataaaa ttgacaaagt
     3181 atgccggcct atattggcga cagagtcaat ccggtaaatc tgaatacgaa aattttttta
     3241 cgaaaagctt cacgtctaat atctatttta aaataaaaat attcctgcta atttggaatc
     3301 gtttccttct tttggtataa taatacctga aattcttttg agaggagttg ttcgtgaagt
     3361 gaaaactact atgaaggatg ttgctgcgtt agcaggagtt ggtgtgggta cggtatctag
     3421 agtaataaat ggagtaaagg tgaaagaggt taccaaagaa aaagtaatgg ctgcaattag
     3481 tgagcttaat tatgagcctg atgaatatgc cagaggtatg aaaacgaata gtagcaatac
     3541 aatagctctt attttaccta cgatttggca tcccttcttt tccgagtttg cctactatgt
     3601 agaggaagct ctcagccaaa aaaattataa gctttttttg tgtaatgcga acggcagtgc
     3661 ggaaaaagaa aaagaatata tccaaatggt caagcagagt aaagtagacg gaattatagg
     3721 tattacctat tcagacattg ataaatatgt atcatcaaat ttaccatttg tcagtataga
     3781 ccgccatttt tcggaacagg tttcttatgt aacggcagat aattatttag gtgggcaaat
     3841 tgccgctcaa gaactactta ctcgtaactg taaaaacttg tgctatgttg ggggtgtttc
     3901 accttaccct aacgaaacaa acaatcgtaa agctggtttt tacaatattt gtgaggaaaa
     3961 taaaatagct gtgaaggtat tggatatgcc tgaaccaatt agcaatttag aggaacaatt
     4021 gctcgctttt ttttcggata atcctgaaat tgatagcatt tttaccatca atgatttcat
     4081 ggctttggat gtgataaaag cccaaaaaaa actagggaag gaagcaccta aggattttca
     4141 aattattggt tttgatggag tcaagctatc gttgcaaaaa gactatttac tatcaacgat
     4201 tgtacagccg atagaggaaa tggccaaggt ttcagtagaa acactcatgg cgatgatttt
     4261 aaatgaagaa ccgcctaagc gtgtggtagt accggttttc tttcacgctg gagagacgac
     4321 taaagagtaa aaaaccggtt tataacgatt tttttgagat aggttgacag aaagtgattt
     4381 caatgatatc ttaaaaccaa ttaattggaa acgttaccaa ttaattgaaa aataaaacgg
     4441 aaacgtttca aaaaatagga ggaacatcga tgaaaaagag aatgttagca attgcagcaa
     4501 ttttagcatt aacagctgga ggacttgcag gatgtggcag taaggagaaa agttcaggta
     4561 gcagtgatcc aggagaaatt aagatttggg ttcagttttc tgatgaaacg cctgaaggaa
     4621 aagcttgggc agagattgtg aacaatttta atgatgaata cgagggtgaa tataaagctg
     4681 ttactgagta tattcctcgt agcggaagcg gcggtggata tgaggataaa gtaaatgctg
     4741 caattacaac caataccctt cctgatgtta ttacgttaga tggaccgaat actgcagctt
     4801 atgctcattc aggagtcatt gctccattag atgattattt gaaagatgcg gacatggatg
     4861 atgtattaga cagcattatt cagcaaggaa cctacgatgg caaaatgtat gcgtttggtt
     4921 tctcagagtc cagtgtgggt gtctattaca ataagtcgct gttcaaagat gctggaatta
     4981 cagaagatca attgccgacg ttagaagatc cttggacttg gacggaattt caagcaattt
     5041 gccaaaagct gactgataaa tacaaaacgc ctgcaattga tatgcaaatg gcgtcgggag
     5101 acgaaatgct gacctacgca tatacaccat ttatatggtc gaatggcggc gatgtcgtta
     5161 atgctgatgg aaccaaagcc gaagactatt ttaacggtaa aaaatccgtt gaagcgttgg
     5221 aatttattca agaattggtt gaaaaaggtt acaccacaat cagtcctatt gaaaaaggat
     5281 ttgaaactgg caaatatgcc atgatgctga gcggttcttg gacaatggca gatttagagg
     5341 aaaactatca agagattgat tatggtattt tgccttaccc cgtttcagat gcaactgggg
     5401 agttagtttc accaaccgga agctggcagt tagcaatgac tgagaaaaca gagaaaaagg
     5461 atgcttctgc ggcctttatt aagtttgcaa ccagtacaga atctagtcgt attatgagtt
     5521 tagggaacag tgttctacct atcagaaatt caacagttga tttaattaaa gatgaagtgt
     5581 cggaaggaat gcaggtatta attaagcaaa accaactttc tggacatgcc cgcccagtgg
     5641 ttgtagctta tccgcaagta tctagagcat tccaacaagc agttcaagat attagctact
     5701 tcgaggaaaa cccagatgta caaaaagtag cagatagtcg cgctgctgaa atgcaacagg
     5761 caattgatac ttcactaaaa tagaagtttc taaagggggc caatcaagtg ttaaaaaaac
     5821 cgacaatgaa agacaatttg ataggttacg gctttctagc gccagctttg ctgctgctga
     5881 cgatttttct gatcataccc gtaatcatgg taatttatta tgcctttact gattattatt
     5941 tgttaacgcc agatgctaga caatttatcg gcttagaaaa ttttaagcga ctgttcgctg
     6001 atccaatttt tgtaaaaagt atttggaaca cggctaaatt tgttgtttgg attattccgg
     6061 tccaaatagg ggcagcttta ggaatggcac ttattatcaa caaaaatcgt aaaggaaata
     6121 tcttttttaa agtagccttt tttgcgccag tcgtgatgtc cttagtcgtt atttcgattt
     6181 tatggttgta cttgttaaat cctaatgaag gtctggttaa tacgctattt gcaaagattg
     6241 gaattgcttc gcagcctttt ttgactagtc ccaaacaagc aatgtatacg cttgttttcg
     6301 tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt tttaggggga atgcaaaata
     6361 ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt ttctaagttt cagcaatttc
     6421 gctatatcac catgccatta ttaaagccca cggcgatttt tattttactt accacattga
     6481 tttcggcctt taaactaatc attcagccaa tggtaatgac tcaaggtgga ccaatgaatt
     6541 ctactatgac aatggtttat tacatttacc aaactggatt tacagaccgt atggtaggct
     6601 actccagctc cattgcgtta atctttacca ctatgattgg tttaattaca attgctcaaa
     6661 gaaaattggt aaaggaggat gatccagcat gaccaagaaa tttggaccgc taatgatcgt
     6721 agaatattgt ttgctgttcc ttttagcggc attattcatt ttcccgatgc tgtggatggt
     6781 agtttcctct atgaagcctg aagcagatgt ctatcaaaat ttagcttcct tcaaagcttt
     6841 tttacctagt ttgaatccag ctaattggtt taaaacttat caagaggtta tttcccggtt
     6901 cagtgtggga acttatttat taaacagtat tttttatggt ttaacctttg cctgtgggtc
     6961 aatcattgta aactctttgg cgggttttgc ctttgcgaag gttaattttt caggcaagaa
     7021 aattatcttt ggagctttgt tagctctgct gattgtcccg atggagacgg ttttaattcc
     7081 gcaatttacc atcgttaatt cactaggctt agtgaatact cgcttggcag tgattttacc
     7141 agcgatggcc agcgcgttta atatctatct gtttagaaat ttctttatcg cgattccaga
     7201 agaaatcatt gaatcagcga aattagatgg tgcaaatatt gttaaaattt tctttcacat
     7261 catgctgcca atgtctaaac cagccatagc cacagtaggt gtcttatcct ttatctccag
     7321 ctggaatgat tatatttggc cgttaatggt cttaactgac agcagcaaat tttcaatgca
     7381 agtggcaatt actacgatca atactacaca gccagtttat atcaatcaag tgatggctgt
     7441 tttaaccatt gcaacgattc cactgattat tgtttatatc gttgcacaaa aatatattct
     7501 gcagggactt ggcggttctg gaacgggtat aaaataggag tgaatagtga atgttgcaaa
     7561 aacttttaaa ggccaatgag tttattgaga aaaatgctca aacagtcaat caagaatacc
     7621 ggaataaatt tcatttgatg gctccgattg gctggatgaa tgatccaaat ggttttgttt
     7681 attatcaaga ggagtatcac ttgttttatc aatattatcc ttacgacagc atctggggac
     7741 caatgcattg gggccatgct aaaagcaagg atctaattca ctgggagcat ttgccagtag
     7801 cactggcacc aggtgaaagc tatgacaagg aaggctgttt ttccggcagt gccatagaaa
     7861 aggatggcaa gctgtatttg atttatacag gacacaatgt tgtggaaggg caagttcgac
     7921 aagttcagtg tttggcagtt tcagaagatg gcattcactt tgaaaaatat gccggaaatc
     7981 caatcatcgc agaagatcac ttagcttctg ttgcaacgac cgaagatttc cgcgatccca
     8041 aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc tactaaaact gcagatgctc
     8101 gtggacaaat tgtgatgttc cattccaaag atttactaac ttgggagttt tattccgtat
     8161 tgctggaggg aactccagaa caaggcattg tatgggaatg cccagacttg tttgaactag
     8221 atggcaaaga agtcttgatt ctttctccta ttcaaatgac tccacaaggc aatgcttacc
     8281 aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga ttggcaaaca ggcaaatttc
     8341 tagtagagaa ttttcatgaa atcgatggcg gtttagattt ttatgcaccc cagacctgtg
     8401 aaaacgaagc aggccaaaga ataatgattg cttggatgca aatgtggcag cgtaaaatac
     8461 ctacgaatga tctgggccat ggttggtcag gttccatgac cttgcctaga gagctgaggg
     8521 tggctgataa tcaattgttg caacggccag tttcatcaat ttatcaagta attgatccgc
     8581 agaaaaggat tgaatgggcg aagactgaag agaatcgctt cttactgaag gacttagtat
     8641 atgaccagca ctatttacgc ttggaaatca atttaaaaca ggcaacgaaa ctagaacttc
     8701 actatgcgaa gggacccgac catacattac tgcttaacta tgatgttgaa cgggagtgct
     8761 ttactgcttc cagggaacac atgccgtatg atttatcagg agctgaaaag gaagtgttga
     8821 aaaagcgcag tgtgacgatt cctttggaaa ataattgtct aaccatcgaa attttccgtg
     8881 atacttcagc tattgagctt tttctaaatg atcgtctgac tatgagtaca actttttatg
     8941 agacagaaaa aggacaggat tttcttttgc tggcagacag tgaagtaacc ttttcattgg
     9001 aaagcggctt aattaatcta tagaagggag agctcagtga tgatttatgg ttcaatcgaa
     9061 gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat caaggaaaga
     9121 gtgagctttc cgacaacgac acctgaggaa acaatacctt tagtcattga cttcttcaaa
     9181 caataccaag cagatttagc agggattggg attggctcat ttgggcctat cgatatccat
     9241 cgcgattccg ccacttatgg gtacattacc tcgaccccga aagtggcatg gcaaaatttt
     9301 gatttcatcg gtaccatgaa aaaagaattt ccgatcccga tttcttggac aacagatgtc
     9361 aatgcggctg cctatggtga gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt
     9421 tactatacga tcggtacggg tgttggcggc ggtgccttac aagaagggcg ctttatcgaa
     9481 gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt
     9541 gctggcagct gtccatttca tggcaactgt ttagaaggga tggccgctgg acccgccatc
     9601 gaaaaacgct tagggaaaaa aggacaagag gttgcagaag atgatcctta ttgggaaatc
     9661 gaagcctcct atattgctca atgtgcctac aatacaacct tgatgttttc tccagatgtg
     9721 atcatttttg gcggtggtgt gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca
     9781 tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt taagcaaata tatcgtgacc
     9841 ccagcattag aagacaacgc tggaacgctc ggttgtttgg cattggctcg agaagcggtc
     9901 ttacattcat aaacgaaaaa aggctcttcg acatttagtg ttggtagaga aagatacaac
     9961 tgaaaattta gtcataacca gttaggcaga gtgcttaact ggtttttgtt ttacttggaa
    10021 gatcaagcct tgaatgagat aagttcttgc tcgaaagtta tggaaatttc gatagccgta
    10081 ggccacttgt attgtgcaat agttttttac aaaaaaaggg ggtgcggata tttttttgga
    10141 cctttttaac aaatatatct ttgcttcttc cgatattgaa gagggcttag ccctcctaaa
    10201 gataacttaa ttcgttgatt attataccaa tgaatataat catccaattt gtttataaac
    10261 tgattgattg ttgtattctt ccagtcacgt tgataaaaca tctcgttttt taatcgtcca
    10321 aagaaacctt cgcacgctga attatctggt gaataaccct ttttagacat agagtgaagc
    10381 aagttggctt tgttccaata gtccaactaa caatagcacc atcgtaacaa tcgacaagtg
    10441 gtgataagta gattttccct gcgggaattc taaattcagt aatatctgtt aaccattttt
    10501 catttggctt ctttgcttta aaattccgat ttaataaatt ggggtgtgca ggagaaattt
    10561 ccccagcata cgaagaatac ttttttcgtc tagaaaattt aacaaataga ttttcctcag
    10621 tcatcaatct acgtacaact ttttcagaaa gctttttacc catatttttt aaagcttggt
    10681 gaattctacg gtaaccatac gcacagaagt tatgattgaa aatcgtgata attaattgac
    10741 gttctttgta atacttatct ggtttctcaa gtgcttgttt ttgataaaaa tagctgcttt
    10801 taggcaactg gagctgttga agaagctctt tcaaggtgaa ctgatttcgt aaggcatcaa
    10861 tcaatatcgt tttttcttga tttgatagac aaagaagatt gacgcctttt tcttttttta
    10921 gcagttcagc cgctccttcg agtaatgttt tttcgagctg taattggtgt acttgttttt
    10981 gtaagtcttc tacttccttt aaaagtagct ctttttgttt ttcctgattt tttaaagtca
    11041 tacgcaatgg aaaatccttt ccaagaaccg tgtttttcca ttgatataga gtaactctac
    11101 taattccttc agattctgct aaatttttcc tattagtttg cggtttatag aattttttca
    11161 acacagcttc tttttgatct tgtgttaact ttaccgcact ttttcggagt tttctagctc
    11221 ctggtgcaag ttcttcacac cgctgccgca aggactctct attaggataa ccaagcatgc
    11281 gaatggttcg tgcataacat tgaccatatt caaaataatg attcaccgct attcgttttt
    11341 gttcttcaga atactttgat ttttttcggt gctcctgatg gagctctccg gattccaaat
    11401 actctttata ccatttccgt agtgcaccaa tcgacggata ccctaattct cgaatggttg
    11461 cagcataaga gcaatcgtat tttaaaaata actgaattgc ccttacacgc tctttgtggg
    11521 aaaacacacg gacattcctc ctaagtttat tttttcgtcc aggattatgt ccgcaccccc
    11581 ttgctttctg atgacttatt ctggttcttt gagtgtcaat gcatagatgt gatgtagcta
    11641 gttagaaaaa aattttgaat actctttttt tctttcagac tcctttttaa gtaagatttt
    11701 gaagtgtgag ttacggggtc agaaacttaa aagtttttga tcctttattt tcaagtctga
    11761 tttggcagct tttcggcagc tcatttattt atggtcagaa atagatgctg aaattttaat
    11821 ctattagcaa atagtccaga acgtatgaag aggagtcgga tatcatttgt cattaacacc
    11881 ataaatattt ataaaattcg tgttcaccta caattaaatt taatatataa tattcgttgt
    11941 aagggcttat aattttttgt taatgaacaa gataggaaga aacatttaat ggacagtttt
    12001 cagagattgt cactagtaaa tcaatttgag ttattggcta aatttgagga ccaaaacaga
    12061 tcttattatg aaagaaaaat cgaaatattg agagaaggtt acgagtatca ttatgatgat
    12121 gaaatttgga gtgatctttc tgagccattt ccgaaagaag attctcgatt tgtattagat
    12181 gtattgaaca tgtatcgaga tatcaatttt agtaaatcaa agctaaactt agatgaaaac
    12241 caaattacta attcatacta tactcatttc ggttctgttg caaagttttc caaaaaatct
    12301 attttagtgt aaaattgaga aaaaagacag agagg
//
LOCUS       DAZKSU010000077        12243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000077 DAZKSU010000000
VERSION     DAZKSU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.078"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2208
                     /locus_tag="ACG8Z3_002248"
     CDS             <1..2208
                     /locus_tag="ACG8Z3_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0824537.1"
                     /translation="ITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKG
                     INPGGFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDS
                     KIRKMQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTT
                     SKTYPVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYT
                     EIADVVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIG
                     KDERNKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNI
                     PLTTVIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGY
                     ASFAFVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNE
                     LSNDTNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDII
                     SSPVTEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTIN
                     DSVSDFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVY
                     TLAKRNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDI
                     LGVGIISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2288..2683
                     /locus_tag="ACG8Z3_002249"
     CDS             2288..2683
                     /locus_tag="ACG8Z3_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824538.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            3008..3625
                     /locus_tag="ACG8Z3_002250"
     CDS             3008..3625
                     /locus_tag="ACG8Z3_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0824539.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3666..3965
                     /locus_tag="ACG8Z3_002251"
     CDS             3666..3965
                     /locus_tag="ACG8Z3_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0824540.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            3999..4166
                     /locus_tag="ACG8Z3_002252"
     CDS             3999..4166
                     /locus_tag="ACG8Z3_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0824541.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4211..4816)
                     /locus_tag="ACG8Z3_002253"
     CDS             complement(4211..4816)
                     /locus_tag="ACG8Z3_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0824542.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            4941..7928
                     /locus_tag="ACG8Z3_002254"
     CDS             4941..7928
                     /locus_tag="ACG8Z3_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFQ0824543.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            8077..8286
                     /locus_tag="ACG8Z3_002255"
                     /pseudo
     CDS             8077..8286
                     /locus_tag="ACG8Z3_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            8421..8603
                     /locus_tag="ACG8Z3_002256"
                     /pseudo
     CDS             8421..8603
                     /locus_tag="ACG8Z3_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            8579..8935
                     /locus_tag="ACG8Z3_002257"
     CDS             8579..8935
                     /locus_tag="ACG8Z3_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824544.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
     gene            complement(9449..9616)
                     /locus_tag="ACG8Z3_002258"
     CDS             complement(9449..9616)
                     /locus_tag="ACG8Z3_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0824545.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(9704..11644)
                     /gene="tet(S)"
                     /locus_tag="ACG8Z3_002259"
     CDS             complement(9704..11644)
                     /gene="tet(S)"
                     /locus_tag="ACG8Z3_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HFQ0824546.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            11629..11754
                     /locus_tag="ACG8Z3_002260"
     CDS             11629..11754
                     /locus_tag="ACG8Z3_002260"
                     /inference="COORDINATES: protein motif:HMM:NF019687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824547.1"
                     /translation="MLFPPFKSSKSRKNPSDKYSYHWDFNTKIAVYYTLIATDAL"
BASE COUNT         4091 a         1929 c         2187 g         4036 t
ORIGIN      
        1 attactaatc tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt
       61 actcctaaat ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt
      121 tggtgtaagg ggattaatcc aggtggcttc gatggggtat atactccata tttagatact
      181 gctgtaaaag agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg
      241 gatgcgaaat gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga
      301 gatagtaaaa ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga
      361 ataatgcctt gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta
      421 caagctgaac taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact
      481 agtaaaactt atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat
      541 ggattatatg ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat
      601 atgggtttag aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata
      661 gcagatgttg ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca
      721 gatggcgcag atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat
      781 gatataaaaa tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga
      841 aataagtatc taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt
      901 cctatctatc aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca
      961 gatggtaaga aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt
     1021 tattttgctg ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat
     1081 tttaaggggg tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca
     1141 agaaatgttt gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat
     1201 atgtcgactg gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat
     1261 caatttgttg aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct
     1321 acatcgggta gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac
     1381 attgccaaat acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca
     1441 caagctggtc ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat
     1501 tctggtttgt catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat
     1561 gatgaatata ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct
     1621 aatactggtt caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt
     1681 ctagccgaca caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg
     1741 acttgctggg gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta
     1801 gtaggtacgg ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg
     1861 gtaaatgttt ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa
     1921 acgtactatt gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt
     1981 ggttcttcag ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt
     2041 gatatcttag gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt
     2101 gggacagatt ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt
     2161 tctgaaaaaa ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact
     2221 atttaaacag tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta
     2281 aaaggacatg atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa
     2341 taattctgga tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt
     2401 aataactgtc aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa
     2461 acttcctgag tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa
     2521 aaaattaaaa gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt
     2581 caaagaaaaa aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat
     2641 ttttaaccct aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg
     2701 taaaagttaa agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa
     2761 agaaaaactg gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt
     2821 attttagcgg aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac
     2881 tttttgacat gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt
     2941 tgaaacgttt ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat
     3001 aaaaacgatg agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg
     3061 acaactgatt tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc
     3121 cagcggcaaa gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg
     3181 agatttactc tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga
     3241 agaatggcga gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct
     3301 gttagatact gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat
     3361 tctgcaatta ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag
     3421 agaaggcgta gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt
     3481 acctcctgaa tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta
     3541 tgctatggat caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga
     3601 acataccttg caccactttt cttgatagat ataacagcta ttagacggga agggagagtt
     3661 tgaaaatgga cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac
     3721 gaacccaagc catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt
     3781 caaaaacaaa tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct
     3841 atacaaaaaa ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt
     3901 tatcagaagt cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt
     3961 tataatcgtt agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc
     4021 gttggggcat tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact
     4081 gtggggaatt agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc
     4141 gccgatacgg ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg
     4201 tagtatattt ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc
     4261 cgttttcttt tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt
     4321 taaaaactaa gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct
     4381 tatatcggcc ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct
     4441 cacgctcgtt ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat
     4501 ccaggagttc atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta
     4561 cttttttctg tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac
     4621 gatctagtga tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa
     4681 actcaggacg ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa
     4741 gtccttccat cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat
     4801 agccaatttt tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt
     4861 acttgtagtt ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat
     4921 ttacttcatg gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt
     4981 atttaggaga acctagtaca gaacaattaa accaattttt ttatttgcat gaaaaagatt
     5041 tagaattgat ggagactata cgacgaccag ctactagatt aggttttgca ctacagttag
     5101 tctcactacg ttttttagga acttttatat tagatcttac caatattccc caaaatgtat
     5161 tgagttatgt agcaactcaa ctaaatattg atcctggaga aattatctac tatagcagga
     5221 gacagaccag gtatcagcac acaaaattga taaaatcaca ttatggatat caagatttca
     5281 accaaccgga aatagaaaca tacctagcta actggctttt aaatagagcg ctgtatacaa
     5341 cagaatcttc agatatgttg tttgatatgc ttctaaaaaa atgtctggat gaaaagattg
     5401 ttttacctgg aattactact ttttctcgtt ttgttgctag tgcaattgaa aaagctgaag
     5461 agcatttata ccgacaattg tctttggttc caactgaaga tgagagaaag cggcttctca
     5521 atcatttaga attaattgga actccagttt atggggcaat aatcaaaatg gacattttga
     5581 gaaccccgtt agctgacgat agtcataaag aaatagccag aggttttgac cgactaaagc
     5641 aatttcaagc tttctctact gaaaaatgga agatggaatc tattcctgaa gggaaaataa
     5701 aagttcttgc aaattatgca tttaaagcta aagctcaatt aattcaacga atgagtgaac
     5761 agaaaaaact ggcgttgtta gtggcattta ttttcatata caaacgtaag gctatggatg
     5821 aacaaatttt agcactatct aatttttttg aaactgtttt tagacgcgcc aaaaataagg
     5881 aatctaaaga acgtcttcgt accataaaag atctagatcg agccgcatcg acactttcag
     5941 atattgtaga tttagtaatg tccagtcatt ctgatactaa tgaactatcc cttcgcgatc
     6001 gtatcttaga aaaatattct gaggaaaatg ttaccgaagc tgtatcgcaa gttcggctat
     6061 tggttaaaaa tgatcgtgaa ccaatagcca tttctgaact tctgaacact tatcgaaagt
     6121 tcagaaaatt tattccggat attttaacta cactaacatt tgacagcaat gactacggta
     6181 acaactgtaa ggaactctgg accttcatta ataatagatt tcctaagccg attacttaca
     6241 aaatgtttgg agctattaaa catactcttc caaagaaatg gatttattac attcatgaat
     6301 accctgaaca aactaataaa tgtgtattga ttgcagctgt cgaattgtta attcaatctt
     6361 taaaacgcca tgatatttat attcctcaga gtgtcttata taacaatcct atggattgct
     6421 tgattgatga gaaaacttgg gttcaacaaa aatacgtttt attagaacaa ctagatcttc
     6481 cttcttcagc aaatgaaatg tctttatggt taaagaacga tttggaactg tcttataaag
     6541 aagcgctaaa tcgttggcct acttctaaaa tggccagaat agaaaatgca gatcgcctgg
     6601 ttgtctctag acttaaaaag acatatgaaa aaaaggaaga taagtctttt agacgtcgca
     6661 ttcagcagtt acttcctaaa attgatttgt cagatttgtt attggaagta aaccaacagt
     6721 taaatcttac tcaatcattt aaccacttaa gtgacaagga caccaaaatg aaagacttgg
     6781 atattagttt attagcagtt cttttatcag agtcctgcaa tattggtttt tccccggttt
     6841 caaaagaagg atttgattct ttaaagtatg atcgtcttgt ctatgtcaat catcattata
     6901 ttcgagtgga tacactttca ggagctaatc aaaaaataat acatgcacac aggaaacttg
     6961 ccacctctaa gatatggggt gatgggcata tggcttcagc tgacggtatt cgttatatca
     7021 caccacaacg ttccctatat tctagaagta atccaaaata ttttggccgt ggtcgtggga
     7081 tcacattcta caattttgtc tctgatcaat atatagggtt ccacggaatg gtcgtttcag
     7141 ggacactgcg agattcgctt taccttctag aaggattcct caatcaaccc agcgaattaa
     7201 ctcctaatca gatcatgact gatacagctg gttatagcga tatgattttt ggtctctttg
     7261 gcttattagg gtttcaattt agtccaagaa ttgccaatga taaaggaacc aaactttggc
     7321 gaatcgacgc taacgctcac tacggggaac ttgatacgtt gtctcaaaat tcaattaata
     7381 tggaactgat ttatacccat tgggaggaca ttcttagggt tgcaggttcg ttgaaatctg
     7441 gaaaagtgaa tgcaacagaa ttaacgaaat ctcttcaacg gaatgggcaa cccacgcctt
     7501 taggaaaagc aataactgag tatggaaaag tttataagac gaaacatcag cttcgttata
     7561 tttctgacga aatatatgca cggcaaattt tagaacaatt gaataagggg gaagctcggc
     7621 atgccttatg ccgtaatatt ttttatggac gaaaaggaaa actctaccag acttattatg
     7681 atgggatgga agagcagctc aatgcattag gattagttac taatgccatc atatattgga
     7741 actcactgta tttagaaaaa gtatttgaac aaatgaaagc tgaaggattc gactgttccg
     7801 aagaaatgat acgcaggcaa tctccactaa ttagtgatca catcaatttt gttggtaagt
     7861 atacgtttag atacgaagct gaattatcca acggtaagtt acgtccattg aatgtagaag
     7921 aattctagca atgttcgggt tttgcatgct accgcactat ttcgtatgac tgttccagcg
     7981 aatcacttcg aatagaaaaa gagaaaaaca cgagaacagc gttaaatcaa cgtttcttgt
     8041 gtttttactt ttgtgctacc gcactatttc gtttctatgt tccatgtacc cctttatcaa
     8101 gaagcgcaaa cacgccaaat tgccgattgg ttgatcggca tgaacgcaag ccctttatat
     8161 accttgaatt tacaacaaaa aggcgtacaa ggtacatttt cattaggacg tgtacaaaca
     8221 ccgactttat atctcattta tcaacgccag gaagcaatag aaaactttaa aaaagaacct
     8281 tttttcctga ataattcata gttttactaa tagtctttcc taacattgat ttaatagtct
     8341 ttaaagggtt ctgttgcaaa gttttaaatc tactatcaaa taaggtagaa taatagaaaa
     8401 agatagcagg aggaatgacg atgaatcatt ttaaaggaaa gcaatttcag caggatgtga
     8461 ttattgtagc cgtgggctac tatcttcgtt ataaccttag ctatcgtgaa gttcaagaaa
     8521 tcttatatga tcgtggcatt aacgtttctc atacgacgat ttatcgttgg gtgcaagaat
     8581 atggcaaact actctatcaa attttgattt caaaccattg ggtcttgcga ttaaagaagc
     8641 ccgtcttagt aaaggattga ctcgtgagca ggtaggatca atgatccaga ttgcgccacg
     8701 ctatttgacg aacattgaaa ataaaggaca acagccaagt tttcatgttg tatatgattt
     8761 agtgactttg ctaaatattt ctttggatgg atttatttta ggggaagaaa acagtcataa
     8821 aagctccaaa cgtcttcaag tagaagcaca gttggattgt ctggatgaag atgaattagt
     8881 gacgattggg aaagtgattc aagcaattta cggagaaaaa tgtaaattta actagttcaa
     8941 taatacacaa tggattaatt aatactatca ggaaagattg attacaaaga tgtaatgaat
     9001 tttttctgat ttttgttaat cataaataga aaatgaattt atctttgtta cacttaggaa
     9061 ctagagcgaa tgataaatag ttctttggtt actcttatcg ctgcaaagta cagttcattt
     9121 tttgttgttg ggaaaatttt ctggatttat tgcatatcac ttcatccgac taaaaatttt
     9181 tgattcccat agcgaagttc gtttttgttc cttaggaaaa acttgactga tatactagtt
     9241 ttgaaatgac gagtaactct gtacttcatc aataatcaag tattgaattt taattgtata
     9301 ctttttatgc ttgagggatg tctactgtaa tacagctact gcaaaaaaaa tcagtacact
     9361 tattttctta taaaggatat caagaactaa gctcaatggt atgcatagaa aagaaaaagc
     9421 cgataaaatg agattttgat atctcgaatt atcggctctg cgtctttgcg tctggctctg
     9481 taattacagt tattctaaac ttcgttattt caattaaaat ttcttgtctg catttaggac
     9541 aaaatagagg gaaatttttt aattcagtat cttccctcat ttttaaacgg gtcttattgc
     9601 cacatatagg gcacagtatc cacctagtat ttacaatatc tatatcctcc tgttctaatt
     9661 aacaactcaa tattcatttt aaaaatttct ctttagttca ctatcaatgt aagttgattt
     9721 tattaaacat atgccttact ttgtctattc tattatttgg acgacgtggt tggaaagcag
     9781 acttaatgtt agtaacctga taacctttta actctgttaa acagacactg cgtccatttg
     9841 taaagaaagt taaactgttt cgatactctt gaatacaacg ggctggaatt tcaccaatga
     9901 gaatgacctc gttacctttt aacttagtat ttaaaatatt tgcacaatat ttggaagcat
     9961 cattatatgc tctcgaaaga tattcttgtg gtacataaat ttcgaagcta agatatggct
    10021 ctaataactc tgtaccactc tttctaaaag cctgctctag tacaataggc gcaagcattc
    10081 ggaaatctgc tggcgtactg acagggctgt aatatagacc atacttaaaa cagatcttac
    10141 agtctgttaa tttccaaccg tacaatcctt gttcacaccc atagcgtata ccttccataa
    10201 ctgcattttg aaatgattga tttaaataac ctagagaaac caggctctca tactgaatgc
    10261 cactacccaa aggaagtggt gttacagata aaccaataga agcccagaaa ggatttggag
    10321 gtacttcgat atcaatggta aattcagatt tttttaatgg tctttccata taaatgacag
    10381 ttggttttct tgtttctatc tcaatatgat atttttcttg aatcagagta caagttacct
    10441 ccatttggac ctcacctaaa aaagatagca caatttcgtg agttactgta tctacataat
    10501 attgtagaag gggatcacta tcggatattt caaaaagtgc atctagtaac ttttctcttt
    10561 gtactgattt acatggttca attgttgttt gtaacatagg aagaggattc tcaagaattt
    10621 ctctatgtgg taatcttttt ttatctccaa gtacgttatt tagttttaaa agctcatttt
    10681 ttaaaataat aatctcacca ggctctgcct tatctatctg gcgtaattct ccatttattg
    10741 aagtatacat ttctgtaact tttatttttt ccttttctga tatattgact gagtctcgca
    10801 aatgtagcgt tccactataa agacgtacat agacaagccg ttgaccatca tctgaatatt
    10861 ctactttaaa aacatttcca caaagtttat ctgaattgag ttgtgtgggt gaaaataatt
    10921 tgctagtaat tacctctata agttgtttaa ttccaatatt attctttgca cttccgtgat
    10981 aaacaggata taaggagcaa ctttgaattc tttcgttttc ctccttttca agttctgcaa
    11041 tattcaatgt tttcccaatg gtatattttt ctaataaata atcatttccc acaattactg
    11101 tctcccattg ttctggttca gtataatcta ttacataagg tttcaaattt agattcacag
    11161 tctgcttaat tatgatgtcg tcagaaagtt tgtccttaat atcttgataa acatctggca
    11221 aatttattcc attttgatca attttgttaa taaaaaatat tataggtatg ttcatctttc
    11281 taagtgcatg gaatagtata cgagtttgtg actgtactcc atcttttgca gagattagca
    11341 aaatagctcc atccaaaaca gataatgaac ggtatacatc tgccaaaaaa tccatgtgtc
    11401 caggagtatc tacaatattt actttaacat tttcccgttg aaaagatgtt attgcggtct
    11461 gaatagtaat acctctctgg cgttccaaaa acatagtatc cgttttcgtt gtaccgctat
    11521 ctacacttcc taactcttta attgctccgc tactgtatag taagctttct gtcaaagtag
    11581 tttttcctgc atcaacatgt gctaagatac cgatattaat aattttcaat tttatttcct
    11641 ccattcaaaa gctcaaaaag cagaaaaaat cccagtgata aatactctta tcactgggat
    11701 tttaatacaa aaatagctgt atactacaca ctgatagcca cagatgcttt atgataaaat
    11761 gataaaagta ttcttaaact gggcacaaaa aaccagtcct tattttaaat aaggatgctt
    11821 aattaattat tcccgctatc taattgacag tttatttaag aataccttgc cgcattttta
    11881 ttatctcctt ttgaatgtta tcttaattct atcatatgat agaatgcttg taaagccaac
    11941 catatttttt gaacgaaagg gaaattaatg agaaattaat ggaagttagt gtagtagttg
    12001 aagataactc ttcagaatgg ctatcaaaat tcagcgattt aataacgatt tacaagttca
    12061 taagggattc tttattcatc cagctattat attacttttc aggtcaatag gacaactgtc
    12121 cacaaccttt ctacaaatat attacgtata atttttgaaa tatatactgc ttaacaaaat
    12181 attttgcagt ggttccaata gttttgaatt gttaatatta agtttgactt ggaaaatatc
    12241 att
//
LOCUS       DAZKSU010000078        12111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000078 DAZKSU010000000
VERSION     DAZKSU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.079"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(314..895)
                     /locus_tag="ACG8Z3_002261"
     CDS             complement(314..895)
                     /locus_tag="ACG8Z3_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0824548.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1078..1704)
                     /locus_tag="ACG8Z3_002262"
     CDS             complement(1078..1704)
                     /locus_tag="ACG8Z3_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0824549.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1726..3057)
                     /locus_tag="ACG8Z3_002263"
     CDS             complement(1726..3057)
                     /locus_tag="ACG8Z3_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0824550.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3212..3613)
                     /locus_tag="ACG8Z3_002264"
     CDS             complement(3212..3613)
                     /locus_tag="ACG8Z3_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0824551.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3699..3905)
                     /locus_tag="ACG8Z3_002265"
     CDS             complement(3699..3905)
                     /locus_tag="ACG8Z3_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824552.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(4000..5502)
                     /locus_tag="ACG8Z3_002266"
     CDS             complement(4000..5502)
                     /locus_tag="ACG8Z3_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0824553.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5520..5975)
                     /gene="argR"
                     /locus_tag="ACG8Z3_002267"
     CDS             complement(5520..5975)
                     /gene="argR"
                     /locus_tag="ACG8Z3_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0824554.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(6073..6690)
                     /locus_tag="ACG8Z3_002268"
     CDS             complement(6073..6690)
                     /locus_tag="ACG8Z3_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0824555.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6911..8284
                     /locus_tag="ACG8Z3_002269"
     CDS             6911..8284
                     /locus_tag="ACG8Z3_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0824556.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8359..9471
                     /locus_tag="ACG8Z3_002270"
     CDS             8359..9471
                     /locus_tag="ACG8Z3_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0824557.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9543..11180)
                     /locus_tag="ACG8Z3_002271"
     CDS             complement(9543..11180)
                     /locus_tag="ACG8Z3_002271"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0824558.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11348..11956)
                     /locus_tag="ACG8Z3_002272"
     CDS             complement(11348..11956)
                     /locus_tag="ACG8Z3_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0824559.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3209 a         2547 c         2179 g         4176 t
ORIGIN      
        1 gagcagctat ttttatttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
       61 tctccactaa acggtgttca atcagctaac cttcggtatt agctcaaaaa acggcaaaat
      121 atgaggaact attttgccgt ttgtttctct aatactcagg tcaaagcgct gatttcacaa
      181 cctctaatac acggtgttcc agaagtagct tcttcgaaaa ataagccgaa atttcacaaa
      241 aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca
      301 caacctctaa ttattaaaga accttttgcg aagttaacaa tctttctttg atcaattgaa
      361 aattctgcct aagtagtgat agatcctgtg tgactttcaa caataagata cttccctgga
      421 tttcagcaat caacgctttg gcttgacggt ctgcttcttc atcggacagt ttttcttcag
      481 aaagtgccag gatattttgt ttcgtatact tgacccattg attgactaat tctagtagca
      541 acagacggta ttcttcatcc actgtactca actcaataat caaattacct acaggacagc
      601 catgataagt gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt tcttctaatg
      661 cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa caattctgtc tgccatacgt
      721 ccatatgcgc atggatcacc gctagacaaa cttctttttt tgaagaaaag taataataaa
      781 attgaccttt tcctgtatca gaagctgcta gaatatctcg gattgacgtt tcatgatagc
      841 cttttttata aataagatct ttggtcgctt ccaagatctt ttcctttgtt ttcaatgata
      901 ttctcctttt tcatttctca tagtcctata ttatacagta aattcatcat tttttcttct
      961 taatcgttta aaaaagactg tgccaaatta tctgacacag ccctcactat aaaactgttc
     1021 tcatatagct agatgttcat tgattcaaga actgcgagga caaaagcaat tcctatctta
     1081 tgatagttgc tctaatactt cctctgttgt catcacttga tctgcaataa agccaaagtt
     1141 cgtcaacgct gcatccattg cttcttggcc aggagcacca attgcatctg tcgctaccac
     1201 aacattgaat ccactctcta ccaattcacg catatgggaa tcgctacaca agtttccgtt
     1261 cattccacca agaattactg tgtcgattcc attttttctc aattggagcg ccaaatcatt
     1321 gctttctggt ccaaagattt tatgaggaga gctgatgatc gtattttcat ccaaataagg
     1381 tttcaattct tcgataaaat cggctcctga accttcttcc actgattcat attgactttt
     1441 tctagcaaac atctgctgct caagcatcat atgttctcct gcaccgccaa attgccattt
     1501 atggtcatgt gggtaaaaat aatgaggaga aataaagagt ttgtatcctt tttcttttgc
     1561 ttgttttacc atttcaatca tattttcgat cgtattgtat ttttctaaga tatcttttgt
     1621 caaaccaaag ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag tgagaactac
     1681 tgctgtattt ttattattga acatggtgga ttctccattc gttatttatt ttaatacatc
     1741 atttagagct tcactcattg tcggatgaga atagatttga tctctcaata acgtataagg
     1801 aagttttcca ttcatagcta gcgaaatcaa gttgatgact tcatgagaat cttccgcata
     1861 gatacttgcc cctagaatca agtcagtttc agggtcgact aaaactttga atacaccttt
     1921 aggatcttct aatacttgtg ctttagggac accgcttgtc atatacttga ataatttgta
     1981 attgacgcct tgtgctttgg cttgtttttc tgttaaacct acattggata aagtcggagt
     2041 gataaataca ctgtaaggca cagctttccg gttattcgtt gttcgtttat tctcgccctt
     2101 caactgatcc ataatgattc gataatcatc tagagagata taagtgaact gcaatccgcc
     2161 tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt catccacagc
     2221 gattgctccg cgatctgtta gtttaacatc tgtattttct aatccaagtg atgctgtact
     2281 tggttttctt ccagttgcag ccaaaacttt tgacgcttgg attgttactt cttggccatc
     2341 ttctgtaaag gtgacggcag ctgttgattc ttggtctgct acttgatcta cagatacacc
     2401 tagatggaat tcgattcccg catttgtcat gtcttctaaa atcatttgcg cgatatcttc
     2461 atcttcacgt ggaagaaatt cagaatgggc atcaagaacg acaactttag atccgtattc
     2521 attgaacatt gatgcaaatt ccaagccaat atatccagcc ccgatgatca ctaatttttc
     2581 cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac ttgtattcaa
     2641 tcctttgatt ggtaaaatga ttggttcaga accagtattg atgaaaatcc gttcgccttc
     2701 aacaatcact ttttcaccat tattcgtcac ttcgatttga tggttagaaa gaaaacgtgc
     2761 tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc atatgataat ttttggcgcg
     2821 aagcatgccc gtcaattttt ctttccgagc aacagcctct gtaaagtcga ttcctctttc
     2881 tccattgaaa atcaatgatt tagaaggaat acagccgatg ttgatacatg ttccgccata
     2941 catacgtgtt gacttttcaa cgactaaaac cgattcacct tttgaagcta acgccttcgc
     3001 taaagtctta ccgccctttc caaaaccgat cacaatattt tgatattttt ccatcatttt
     3061 tccctcgctt cttttttacc taccgattgg tacaaaaata tgataaccga taattgtcta
     3121 ttctccaaat catctgcttt atttgtcaaa aaaaaccaga caaaaagcgg tcctttcacg
     3181 tttttagtct ggttcgattt cctttattct tctaaacaat cgttacttct acatcgatat
     3241 ttcctctagt tgctttggag taagggcaga attcatgcgc atcttctgca attttttggg
     3301 cttcttcttc gctgatgcca tcgatatgca cttctaatac tacaccgatc tgatagccgc
     3361 catcatctaa attataaaga gagacacgtg cttttactgt ggactctgct gtcattcctg
     3421 cttctttgat catatgttgt accgccccat tgaaacaaga agcataacct gctgcaaata
     3481 attgttctgg atttgttcct tctgcatgga ttcctggagg agtgattttc atatgcatcg
     3541 agccatttgg tgcctcgact tctccattgc gcccgccatg gttgatcatt gctgtttcgt
     3601 atgctttttt cattgtactg ctcctttcga ttgaccattt gctacttggc tataattata
     3661 gtgtatgaca agtaaggcgc tttctacaaa aattctgctt attcgaatac cagaatgccg
     3721 tcttctacta tttttccaaa aaacaaaaaa atactactcg tgaacaacag cgtcatattt
     3781 gttccaaaat aaatagcaat cttttctaca taatctttta tatgattgat tttaagatac
     3841 atatatacca gaaagttgac cccataaacc aatactgtta aaaaaataac catattcgtt
     3901 accatttttt cctccttttg ctggtctccc taccagcact cgatttaacc ataccacttt
     3961 ttttgccgtc caagcaacta gcctttcaaa aagactggtt tattctgctg ttaatgattc
     4021 aagatcagaa aaatgtccga ataattttgc taattttact aacgccaact tgttctttcc
     4081 gaattcatca ttttctatct gtaatactag taacggctca tgtaagctta aacggatcaa
     4141 aaaccagccg tttccgtatg gctcttctag atcatatcga ataccttctt cattttccgg
     4201 gtcgaagttc atcccttttt cttcttccaa cagtcgcttc atttcttcta atatcttgtt
     4261 tccttgttcg cgtggttcat cgctgaacaa ttttaatcgg atttcttgta cttctacggg
     4321 ctgtttcaac tctttgattt gttcatctaa tgtgatacct tttcttttta attctggcaa
     4381 aagcatcagg atctttgcga tgacatatgc accatcatcc agaaaatagt tttccttgaa
     4441 tgcagcgtgt ccgcttgttt cgatggctag ctgtgtatct ttcccaagac ggttaagttc
     4501 taatgcttta ttgattacat ttcgatagcc ggaaatataa cgaacttgga tgcctccttt
     4561 ttcttcaatg aatctttgta gatgagaaga agtaggggaa ttcgtcacga ttcttgttcc
     4621 tggatgctct tttaatacga ttgtacttaa tactgcgatc aaattatttc gattgatcac
     4681 tgttccagaa gcactgacaa ctgcagaacg atccacatct gtatcgaaaa tgatcccaag
     4741 atctgcttca tttgctaaaa cagcctgctt gatactttcc atcgcttctt tattatcagg
     4801 atttgggata tgattaggaa atcggccatc tggttccaaa aattgggaac ctgttgaatc
     4861 cgcacctaag ctattcaata ctttttcagc aaagaatcca ccagcaccat ttccggcatc
     4921 aacaatgatt ttccaaccat ttagtggttt gctttctagt gttccaatcc cctttttaat
     4981 ttttgtgact aaatcttgcg cataaggtgt caaaaggtct gctttttcta cgcttccagt
     5041 ttgttcgctt tttatttttt gctgctgtga taggataaaa gcaatatctt ctttttctgc
     5101 tcctccttct gaggtaaaga attttattcc attataataa tagggaagat ggctagcagt
     5161 cagcataacc gcagcatcgc acgagaactg ttcaaattga gtggacataa acatagcagg
     5221 cgtagtagct aattcgaaat cgatgacatg tatcccttgt tctttcagac tttggatcaa
     5281 cgcctctttc agtgctggtc cgcttatcct actgtcatga ccaatcccaa tcgtccattt
     5341 tttatttgga tcggttgatt tcttcttttt ctttatccag tttgctagtc cccagccgat
     5401 cttttttacc atgtctgtcg tcaagtttac ttcatatcct tcaactggta atgcgattcc
     5461 tcgaatatcc gatccatttt gcagcgcttt catatctttc atataaaagt cctcctcatt
     5521 tattccctca ttttttccag cttttctgca aatcgctgtg cttcttcttc tgagcggcaa
     5581 ataataatga atgtatctac accggccatc gttccgacaa cttcagggta agccacttca
     5641 tctagccaat tcgttaccac atcggcacca tttcgttcag tcaatacgat aaccatgaac
     5701 tgaacagact ggatacgcaa aacctcgcgt ctaattgctg tacgcaaacg ttcttcattt
     5761 aaagaaacag atggctggtt aaataatgta tagcgtacta ttttgttttc attttgactt
     5821 tttactaact tcatttctcg aatgtctcga gaaatcgttg cttgagtcac atcggcgcct
     5881 gcttcatgca gccgcgcaag cagctcttcc tgtgtcccaa taggttgttc tgtaatgatc
     5941 tggcggatca gtgcttgtcg ctgtttcttt ttcatattag acacctctca atgttgatta
     6001 tagcaaaaga taagtaatat ttcttcatat tttggtattt tttactttta ttttttgttt
     6061 tcattcaggt tgctaacctt tttctatttt aacacgcaaa atcgtcttca ttttttcagg
     6121 tgctgatttt cttggatcgc tgatgtaaat ctctttgtgt tctttagaag ctctttcata
     6181 gccttctttt gcgatgaatg cttctaattt ctcaaacgtt gctggttctt cggcataggg
     6241 cccgacgtgt aagatttggg cacaaagacc ttcttccatc tttttgaata ctacttgttc
     6301 tttcaagtct tccggaagtt tgctagaacg ctgaatggct tcttgtgcta tcgcttctgt
     6361 aacaaagtca ggctgtcgga tcatcaattg ataagaaaaa tgttcttttt tcatcactgg
     6421 ttcatgcaaa tacttttctt gcagtcccca ttgtccttcc agtggataga cagtatacat
     6481 ctgataatca ggaatcagcc aatttttttt aggcgacatg cgtattgcat agctgactgc
     6541 atatagacag gctactcttc gtttaaagtc ttcctgattc gggtcgccga tccctctgat
     6601 gctaaagaat gattttttag gaacctctag tatttgcggg ctttttttca tactatagct
     6661 cttgtcttta cgccattcaa ttttttccat ttttcttcct ccgcgaaatt ccatttagtt
     6721 atttattatc atacctcttt ttgagtgact tggctaatcg tgttgacact atttttcagt
     6781 ttgtgctaga ataagtgaag taaataaatc gtacagataa cacgtttcgg cgtgtcgaga
     6841 gagaggcggt ttctattttt ataatagaaa tcactctctt tttttacgaa attttttaga
     6901 ggtgatgtaa atgacaaaac aaaaacagct gcttttagta cctgcagtaa ttgcaacagg
     6961 gatgatgtct tttgcgggcg tattgatcga gacggccatg aatgtaactt tcccaactct
     7021 tatcagacag tttgatttaa cgactgcaca agtccaatgg gtaacaacga tttatctttt
     7081 gatgatttcg atcattgttc cgctgtctac ttatttgaat cgcaatttta gtctcaaaaa
     7141 actcttttta gcttcaaatc tgcttttctt ggcaggtata atgatcgatt tcttctctcc
     7201 taccttcatg gtcctattgt ttggtcgatt gctccaaggt gcagcgacag gtatcgctct
     7261 tccgttgatg ttccatatta tcttgacatt tgcccccctc aatcgacgag gcgcgatgat
     7321 gggtgtcggt actttgacaa cggccattgc tccagcaatt ggccccactt atggaggcat
     7381 tatgacttca cattttacat ggaatcatat cttcctgttt cttgtgcccg ttttgtttct
     7441 ttctttgatt atcggctggt ttgctattcc cgagatggca gtacaaaaaa gtggttattt
     7501 ggattggata agcgttttag gaatttgtct tttgtttagt ggaagtctaa catttttgag
     7561 tacgttggga acagtttcca gctggattgc tttaatcatc ggcggtattg gctggttcct
     7621 tttttactat cgaagtaaaa aagtggagca tccactgatc cgactgtcta ttctttcaaa
     7681 taaaacattc cgcttgtttt tgattggctt cttagtttat cagtttttat tacttggtgt
     7741 atcttttgtc ttgccgaatt tccttcagat cgttcaagga agtagtcctt ttgtcgctgg
     7801 attagcgatg cttcccggcg cagcggttgg tgctttgctt tctccttttt ccgggaaaat
     7861 gctggatcaa tatggtccga agaagccaat ttttgctggg ctgattcttt ctttgattgg
     7921 ttggctagca ttattcctac tcataggaca cgcttcagtg ggtgtattga ttgcttgtca
     7981 tgtgttttat atgatcggta tcggattatc ttacagcaat atgatgacca ccggaatgaa
     8041 tgcactgaat gaagaactac aaggagacgg gaacgcaatg ttcaatacct tgcagcagtt
     8101 ttctggcgct gtggctactt cattagtagc ggtcattatc aattacgtcc agcatcatac
     8161 ttcccataac tatgaagtgt ctactacatt aggatcaaaa gcagcattag gcgtcttgtt
     8221 gctgttgctt ctcgtaagct ttgctcgatt cgcctattat ctgttctttg ctaaaaaagc
     8281 gtagattcca tatacggcag gtgttgcatc tgccgttttt ttgtgtgaaa atggttgttt
     8341 gtaaaaggag gcttttttat ggaaaaaatt gccattatcg gtggcgggat cattggtatg
     8401 accctagcca gttacttaga tacatcaaaa ttcaatgttg ctttatttga tgaagggaca
     8461 ggacaggcaa ctaaagctag cgctgggatt atctcccctt ggttgtcgaa acgcagaaac
     8521 aaacaatggt atcgtctggc taaagacggc gctgcttttt tccctaaact tataaaagat
     8581 ttccaacttg atcaaacgat ttacgcacaa tcagggacac ttttgcttcg acctgaaaaa
     8641 gatttagctg aattggctgt tcttgccgag aaacgaaaag aagaagcgcc agaaattgga
     8701 aacatcgaat ggctttcacc tgaacagaca tctcaagcac ttccgttatt gaaaccagca
     8761 gtttcactgc gtatcagtgg tggtggtcgt ctggatggaa aagcatattt agaacatctt
     8821 ggtcagcgtt tagcacaaaa acaaatggag cagatccagg caaaagtcac tttgcataag
     8881 aagagcgatt cttggctcat caagggaccg tttggagaat acacagctga tcgtgtaatt
     8941 ttgacacctg gcccggcact taagccttta ttacaagaat taggctatgc tgcagatata
     9001 cgtccgcaaa aagggcagct cttagcattt cagacatctt ataagatgag tcaagactgg
     9061 cctgttgcta tgcttgacgg agaagctgat ttcattcctt tcatggatgg aaagatcctt
     9121 ttaggtgcta cccatgaaaa cgatcaaggc tgggatttgg ctcctactag cgaagcttat
     9181 aagggattga ctaagagtac agaacagttc ttgactgatc cagatcagat aaaaaactgg
     9241 ccatacgagt tccgtgtagg tacccgtgct tatacgtcag atttttcccc atttttcggc
     9301 gtactgccag atgatccaag cttactggct gctagcggtc taggctcttc tggcttgacc
     9361 acaggtcctt atatcggcta tctattagcc aacttcttaa atactggtca ttggcctacg
     9421 gaagattatc aaaaggacat ctccacctat attacgaaaa atccatcata gaaaaacagc
     9481 tttttcaagt aaggatcccg agcgatattg cgcatggatt acttcttgaa aaagctgtta
     9541 ttttagttca atttctgaat caaattctcc aaactttctt ttgcttcatc tggcaagaga
     9601 tcttttccta tctgagcttc aatgaatcgt aaacgaatat caagtcgttg tttcaccaga
     9661 tttttatagg acgtatcctc aaaagaaaca ggaaaatctg gtataggtaa atagtggatg
     9721 tcttcaaatg gaccaaacga cttgaattct gccgtttgtt gcacgattct ttcaacgatt
     9781 gacaatgtcg tttcaggttt gattttttta tcatcgaaat atccagtatt caggacatag
     9841 caggctgttt gctgtttcaa aaacaactct cgaaaagctt gatagtcttc cctaagagga
     9901 tagacacgga aaggattagc aaatggttca atgactagct catctagctg tctttttgta
     9961 gtgatatttt ccgcgtctga tcgttttgtt gctaatgttg caccaaaaga cgctgctaaa
    10021 actgggtcat tgatcttgat aattggcgga agcgaatcat ctttcatgat ccaaaaaaca
    10081 gcatcgatct tttcgctaag atgatcgact cggtcgggtg tcgcataacg tgatttgact
    10141 gtccgcccat ttccgtttcg tatatctcct gttactagga ctttttctcc tttacttgta
    10201 catgtcacgc ccacattctg acaagtaagg aaagaagaaa tggccggatc atcagacgga
    10261 tagtcttgtg ttttatcaaa gtaagaaggt tccaaggcag tagcaaaacc attttttcga
    10321 gaaataacga aagcatcgtc atgcaagatc gaaacagagt gaccttccgt atgttttgct
    10381 aaggtaatcg ttgattttcc agaaccggac aacccaaata cagccatcgt ataggtctgg
    10441 tcattactag catattgttt catcccaccg tggcaggcaa taaagccatt gcgatttccc
    10501 aatgcccaag caagcgtcaa agtcgctttt tttaattctc caaagtaacg taatcctagc
    10561 acagccgctg tattatggag agcatcaaac aaaatgagtc catttggaaa tgtttcatgt
    10621 gtccattcgg gatcacaaaa gatataaaga tctccctcat caaaggaaac agacttttga
    10681 taagtctcta agatcttcga attcgctggc tgaaaattca gcaaataaga atacaaattg
    10741 ttctcaaatc cttctggaat cagcaaatga ctttgcacag aaaattcttc agataggcca
    10801 acgattactt ttgtatggta aaactggcgc ttgcgacttt gatacacagc ttctcttaaa
    10861 attttctcgt atttgctttg ttccttgtct aagtcctcaa agatccgccg agcacttgct
    10921 gttcttccca caatgcttcc atcattgaat actagtgtcc taacatcagc tggtaaatct
    10981 agttcttcag catgtgagat tgttaaatct gtgatgatcg ttcctggact cccttttgct
    11041 agttgataag ctgtcttcct atcaaaaact tcatgaacat tattaccgta aaatgtcgtt
    11101 tctacaatcg atcgaatact ggataacagt ggatttccac ttgtgatttc gtttaattcg
    11161 agactctctt ttgtactcat ggctcctcct cattccctaa acaaaaaaac gttttcttta
    11221 ttatacatta ttcgagtttt tatttgtact gctgcgctgt gggaagcaaa cgagttattt
    11281 ttcacacaaa aaaaccgaga acttgaataa acaagtttct cggtttcatc attttccttt
    11341 tatcggctta gtaccagccg tttgctaacc agaatgtttt agcagcatcc catgaaccat
    11401 aacgagatgt tacatattgt tctgctacac gttcttgatt ggcagctgaa tagtcgccgt
    11461 ttaagtatga agcatctaat tgataacggc cgatgtaacg tccgttagta gctgtgtatg
    11521 aaccgcctga ttctttttgt gcgatccatt cttttgcaga gcttgaagtt ggcgctgttg
    11581 cagcagctgg ggcagcagga gctacttcaa ctgtttcaac aggtgcaacg tatgtatgtt
    11641 cttgcgttgc tgtttcagtt gtagatgctg tattttctgt tgttttgttt tctgttgttg
    11701 ctgaagaatc atttgaagtt gggattgtta attgttcacc aacataaatg cggttgatgt
    11761 ttgcgatcga atttttttca gcaattgcat cgatcattga gttgtctcct gcaaattttt
    11821 gtgagatctt agataatgaa tcacctgatt ttactgtata aacttcatca gcgtgagcat
    11881 ttgtagttcc tacaaacatt gcagtagcac cagcagcaaa agttgtacct aaaacaattt
    11941 tcttaaatga attcatatta aactcctttt tttctccgtt ttttattctt tcgacgctat
    12001 taactttatc atttagaaat atttcatagg taaaagaaac atgacattta atgacaaaat
    12061 aacgaattat ttaatatttg taacatttaa acaaatattt ttttgataca a
//
LOCUS       DAZKSU010000079        12007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000079 DAZKSU010000000
VERSION     DAZKSU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.080"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..961
                     /locus_tag="ACG8Z3_002273"
     CDS             65..961
                     /locus_tag="ACG8Z3_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0824560.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(1104..2045)
                     /locus_tag="ACG8Z3_002274"
     CDS             complement(1104..2045)
                     /locus_tag="ACG8Z3_002274"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0824561.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2171..3292
                     /locus_tag="ACG8Z3_002275"
     CDS             2171..3292
                     /locus_tag="ACG8Z3_002275"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0824562.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            3550..5052
                     /gene="eat(A)"
                     /locus_tag="ACG8Z3_002276"
     CDS             3550..5052
                     /gene="eat(A)"
                     /locus_tag="ACG8Z3_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0824563.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            5119..5646
                     /locus_tag="ACG8Z3_002277"
     CDS             5119..5646
                     /locus_tag="ACG8Z3_002277"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0824564.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            5858..6919
                     /locus_tag="ACG8Z3_002278"
     CDS             5858..6919
                     /locus_tag="ACG8Z3_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0824565.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6922..7818
                     /gene="murQ"
                     /locus_tag="ACG8Z3_002279"
     CDS             6922..7818
                     /gene="murQ"
                     /locus_tag="ACG8Z3_002279"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0824566.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            7832..9814
                     /locus_tag="ACG8Z3_002280"
     CDS             7832..9814
                     /locus_tag="ACG8Z3_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0824567.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9884..10330)
                     /gene="nrdI"
                     /locus_tag="ACG8Z3_002281"
     CDS             complement(9884..10330)
                     /gene="nrdI"
                     /locus_tag="ACG8Z3_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0824568.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            10541..11965
                     /locus_tag="ACG8Z3_002282"
     CDS             10541..11965
                     /locus_tag="ACG8Z3_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0824569.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         3810 a         2125 c         2646 g         3426 t
ORIGIN      
        1 tggcttttta ctatttaaac aggctatcat aggaaaaagt gacagagaaa gtgaggcgga
       61 acagatgaac aaaaacgacg tattgctaat tgcgaaaaat aaatcacttt atttttttcc
      121 ttccatccgc tctttagatg tttggtccgg aaaagattta ctacagtcgg aattcattaa
      181 atcagctcga caacagttgg caaattatca gctgatcgtt tttttagatt atggatttga
      241 tatggagatg gctcgacgcg tgcgtccatt taccaatgca aaaatcgtgt tatttttttg
      301 gaaccacttt aaagcagaac acgaggcgat gctgaagcag gcacaagatg aaccggcaat
      361 cgatgagatt tatcattttg attttttaga agcgaaagaa cttggactaa aacataatag
      421 ttccttttat tcgaaacata tggatttttc ttctagcgaa ccaaagacgg atctattttt
      481 tggtgcaaca aataacggca gaaaagaacg ggcagaaaaa tataagcagg aatttacaaa
      541 aagaggtgtt acgaccaatt actttattct tccgcttcga ggaaatgaac aacctggata
      601 ccttacttat gaagaatatc tgaaaaaaac cggggaatcg agggggattc tagaactgtt
      661 gcgggaaggg cagcaaggag tgactttgcg aacgtttgaa tctatgtatt ttcaaaagaa
      721 actagtgaca gataatcaag ccatagctca ttatcagttt tatcattctg aaaatatttt
      781 tttgcttcaa gaacgtgatt tagatgaatt accggaattt ttgcatacgc catttcaacc
      841 aatcccatca gaactgttgg aattttttga tgcagaaaat tgggcaaaac gttttttcac
      901 tactgactcc gctatttatg aacaatatga atacgatctt tcctctgtta aaaataaata
      961 aaagtgccaa ccaatcatag atgaaaataa acaagcgcaa ttcttatggc aacatgagaa
     1021 ttgtgttttt ctttttgaac tttcatgggg gtaaaaaaat gagaaccagc tgctgatcca
     1081 gctgattctc atcagagagc cttttattct gcgccaagga ttccttcttt tgcatggact
     1141 aattgggtca aaaaggcaca atatggtgta gcaatatcat atttttgtcc ttttcgccaa
     1201 acggcaccat tgatgtagtc gatttcagtc aaacgatggt ttttgatcag gtcttggtac
     1261 atagatgggt aatgcaaccc gatgttgtca ggatcataag tagaagcaat atgttggtac
     1321 acttcttctt gatctaatgt gattccttct tttgctgcga tagaagcaaa ctcagataca
     1381 atcgttcgaa ccatgccctc tgccgctttt tgcgcaccta attcagcaat attacaatcc
     1441 aatatcgtgc ataatccatt taatgttccg tttacacaag cttttcgcca aatggaataa
     1501 cgaacattat gactatagat tggattcaat tttgcttctt gaaagacttc tacaactttt
     1561 tttgtaaatg cttcgccttc tggttccaag ttctctagtt cgatttctcc atcacctaat
     1621 aatgtgactt ttccgggacc agctagacca gctgtccaca tcgtgatccc aaataagata
     1681 tttttctttg gtacatattt ttccagtaca tcttcatgac ctaatccatt cagtaaacac
     1741 aaaacataag tattctcagt aataatggat tggatcgaac gaaacatatc atctaattga
     1801 ttcgcttttg tcaaggctac gatcaaatcc acgtgtttgt tgctttcgat gatttcttct
     1861 ggtgaataga ttggcagttt tgcaactact tctttgccat taaagtcagc aatcaatcca
     1921 tttgtacgaa tggcttcaat gtgagcaggc cagcgatcga tcaagacgac ctcatttccg
     1981 ctttgatgca acattaatcc gattcggctg cccattgctc cggctccagc tattgcgatt
     2041 ttcatgtttt ttctctcctt tattcaatga gttccctttt agtatataga aaaacagaag
     2101 aaaatagcat tgttttgttt gaaaaaagtg aaaaaaagtg tcttacttaa gaagaaagtg
     2161 agggatatag atggatattt tggcggcaat cgattcattt aaaggaagtg cttcttcatt
     2221 tgaattaaat caggcagttc taaatggttg ggaagaccgt aaaggaagaa aaatcaatgt
     2281 gccgattgca gatggcggag aaaatactgc agaagcaatc tattacgcgc tcgggggaac
     2341 ctggcgtacg attgatggga tggatctctt atttcgtcag cgaaaaatca gttatttatt
     2401 gacgaactgg caaggaaaaa ataccgcagt cattgaatct gcacaagtat taggattaga
     2461 tttacttgat aaaccaacgg atcaaaccat ccgacttgct tctagttatg ccttgggaga
     2521 gcttatcaaa gatacactgc ttcaagaagt ggaacaaatc attgtcactt taggaggaag
     2581 cggatgtagc gatggtggtc taggattact ccaatctttg ggtgcttctt tagaagggta
     2641 cacagaagga aatcctttgt tatctactag aaaacttcat ttggatcgaa ggaacgttct
     2701 tgtttctaaa gtaaaagtga tcatcgctgc agacgtgacc aacccatata caggtcaaca
     2761 aggagctgct tggcaatttg gaagacaaaa aggaggaacg gatacgacgt tagctttctt
     2821 ggataaacaa gctgaaaaga ttgctcagca gataaaagat gtttgggagg tggatttaga
     2881 tgacatccca ggaagcggag cagcaggcgg tattggcgga gccctttttt tgttaggagc
     2941 gaagatggtc tctggttttg agttggtcag ccaaatcact ggattagaaa aacagatcga
     3001 acaagcggat gtcatcatta caggagaagg gcggattgat cagcaaacaa tccacggaaa
     3061 agttccatat ggcgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg
     3121 cggaagtaga gaccgagagc tggaaggact ctttgatttt ctccccattg tcttaagcat
     3181 tcaattagga ccaatcgatt tagaggaagc aatggaacac aggcaaacat tagaaaaaac
     3241 agaaatctta ggacagacac tcagcagatt tttcaacatt ctcccgaagt agttgacaaa
     3301 aacaatttct ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt
     3361 gaaaaattaa gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaagggag
     3421 cagtgtgcag attttcggga ttggcttggt tctttcgatg tatgggtctt tttgtgtgcc
     3481 ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt tttacgaggt
     3541 gatggaataa tgtctaaaat cgaaataaaa aatctgacat tcggctatga cagccaaggc
     3601 acattattat ttgaacaagc caatctaaac tttgacacac aatggaaact aggacttatc
     3661 ggacgaaacg gtcgaggaaa gacaacttta ctgaatatcc tgcaaaacaa actcccttat
     3721 caagggcaag tgatccatca gcaagcgttt gtctatttcc cgcagcaaac aaaagataaa
     3781 gagcgtttga cctattatgt gttaaatgag attatggatt ttgagatatg ggaaattgaa
     3841 agagagctcc aactgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta
     3901 tcaggaggag agaagacaaa agtcctactg gcactcttat ttgtggatga cactcatttc
     3961 ccgttaatcg atgaaccaac gaatcatttg gatatctctg gcagaaaaca agtagcggct
     4021 tacttgaaaa agaaaaaaca aggattcatc gtggtgagtc atgatcgggg atttatcgat
     4081 gaagtagtgg accatgtttt agcaatcgaa aagagccaac tggaacttta tcaagggaat
     4141 ttctctatct atgaagaaca gaaaaaactt cgtgatgaat ttgaaatagc taaaaatgaa
     4201 aaattgaaaa aagaagtcag caggctaaag aaaacagcag ctgaaaaagc agaatggtct
     4261 cgttcccgag aaggggacaa aacaaagaaa aaagtcggat tcatcgatac ggaatctagg
     4321 cgagtgaata aaggagcagt aggcgctgat gctgcacgga cgatgaaacg atctaaagca
     4381 atcgtgaatc ggatggaaac ccagatcagt gagaaagaaa aattattaaa agatatcgag
     4441 tatatcgatg ctttgacgat gaatagccaa gtgtctcatc ataaacggct gttaagcgta
     4501 gaaaatcttc aattagggta tgaaaatctg ttattcgagc cagttcattt tacgatcgaa
     4561 cctcatcagc ggatggcaat ttcaggtcct aacggcgcgg gaaagtcatc cattatccat
     4621 tatcttctgg ggacattcaa cggcaaggtt gtaggagaaa aatcccagcc caaacatttg
     4681 accattagtt acgtacgcca aaattatgaa gacaatcgag ggacattggc ggaattcgcg
     4741 aagaaaaacc gagtagacta tcaagcattt ttgaataatc tccgaaagct tgggatggaa
     4801 agagatgttt ttcataataa aatcgaacag atgagtatgg gacaacggaa aaaagtggaa
     4861 ttggctaaat ctttatccca accagctgag ctctatatat gggatgaacc attgaattat
     4921 ttggatgtct tcaatcaaga acaattagaa caactgattt tgaacgtgaa acctgctatg
     4981 ttactagtgg aacatgatca aacctttcta gataaagtat ctactgagat tatttctctt
     5041 gagaaaatct aaaccttgta atctcaaaga caacaaggta tggttaagga gaactttgtc
     5101 aacgaaggag agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca
     5161 caaggattag tttgcgggcg agcagatgtg ccgctgactg aaaaaggaca gatgcaggca
     5221 caggcactag cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc
     5281 cctcttcaac gagcaagaga tacagcacaa gcagtagttg atcgtctcaa tttgccgatg
     5341 accgctgatg agcgattggt agaaatggat tttggggatt atgacggact gccaagcaaa
     5401 gatgaaaact ttcaaaaagc gcgtttagcg tttgctgtac gtttcccaaa tggagagtcg
     5461 gtcttagatg tgtacgcacg gatcgttcct ttattgaaag aatgcatgga agacgaagaa
     5521 aatgtctact tgttagtctg tcataatgcc ttgataagag tgatcaatgc ttattttcat
     5581 ccaatgccta atgaagggtt ctttacattc atggtagaca atacagaatt aatttcttat
     5641 gagtaattag acttttgtgt tatttaaata aaatgtttca aggcactgct tttgcttagc
     5701 agtgcctttt ttgtggctgt gtcaattctt ttggcattat aaatccaatt tttagaaata
     5761 aaatttaatt attttaacaa aagtattgaa attttgtttc tttagatata tactctagtc
     5821 atagaaatga agacggatac agaaaaagag ggataatatg tttggctttt caatatttat
     5881 gaatgattct ttgtcagaag agaagaaaca gtatatccgc caaatggcat ccaatggctt
     5941 tgtcggtatt ttcacttcca tgcatatacc ggaagatgat gtttcggctt atcaaaatcg
     6001 attgatcgaa ctaggaagcg aagcgaaaaa gaataaacta gagttgatgg tcgatatttc
     6061 aggagaggca ttgaaacgag ctggcttttc attagaagat ctaacaccct tgaaagcaat
     6121 tggtgtaaca gggttaagaa tggattatca tatttctaat cagcagatcg ccgactggtc
     6181 acatcaattg aaaatcagtt tgaatgccag cacgatcacg cctaatgata tcgatgagtt
     6241 gaaagaagca gcggctgatt tctcacagat tgaagcttgg cataattatt atccacgtct
     6301 tgaaacaggg ttggacaaag aatggtatca ggaaaaaaat caatggctca aaactcaagg
     6361 attccttgta caaggctttg taccaggaga tacagagttg agaggtccac tgtatcaagg
     6421 attaccaact ttggaagaac atcgagggat ccatcctctg gcaggtgctc ttgatctatt
     6481 agcaagtaac acggatattg tttatatcgg agatgcaggt ctgtcagaac acgttcagga
     6541 gcaatttgct tctttccaaa aagaacagac agtcttgctc catacggaac ctgtagatga
     6601 agttttttac gaatatatat taggaaaaca tacgaatcgg caagatgatg caagagatgt
     6661 catacgaagc gcagatgcgc gttttcgaga gattccgcca atcccagtaa gaaatacagc
     6721 taagcgtatg aaaggatcta ttactttaga taacgaaaag tatttgcgct atatgggtga
     6781 gatccaatta actaaatatg atttgccagc ggatgaaaaa gtgaatgtag tagccaaagt
     6841 catcaaagaa gaactaccgc tgatcaacca aatcaaagct ggtatgaact atcaatttat
     6901 aagaaaagaa ggaagataaa aatgagcaaa atcaatttag agaacctaac aacggaacgc
     6961 agaaaccaag aaacttttgg attagatgaa atgagtattt ctcaagcagt ccaactcatg
     7021 aacaaagaag atcagaaagt ggccgctgct gttgaaaaag aactgccaag tatcgagcca
     7081 gtgatcgcca acacgattga atcctttaaa aaaggtggtc gcttgattta tatgggagct
     7141 ggaacgagtg gtagacttgg tgtattagat gctgctgaat gtgtaccgac attcggtgta
     7201 gaaccggaaa tggtcatagg cctaattgct ggtggagaag aagcaatgac tgtagccgtc
     7261 gaaggagccg aagattctta tgaattaggg caagaagatc taatcaagct gaatttaaca
     7321 aaaaatgata tagttattgg tatcgcagct agcggtcgta caccatatgt gattggtgga
     7381 ttagattacg caaaatcgat tggtgctgtt acaggaacga tttcttgcaa taaacaagca
     7441 gaaatcagcg agcatgcgga tttcccaatc gaagtcgatg caggaccaga atttttgaca
     7501 ggctctactc gattgaagtc aggaacagca caaaaactga ttttgaatat gatttctact
     7561 atcagtatga tcggtatcgg taaagtattc aataacttga tggtcgacgt caaaccaaca
     7621 aatgaaaaac taattgaacg ctcaaaacga atcatcatgc aagcgacaga agctgactac
     7681 gaaacagctg aaaagtactt tgaggaagca gatcaaaatg tcaaactggc tatcgtcatg
     7741 attttgacaa atagtgataa agaggcagct caagcaaaat tagctgaagc aaacggtttt
     7801 gtaaaaggaa ccttatgagg gggaaaagac catggcaaaa aacgaattga ctttggaaga
     7861 actggcaaaa caaatttatc aaggagcagg cggtatgggc aatgttgaaa gcattgtcca
     7921 ctgcatgact cgtgtacgta tgaaggtcat caatgatgat cttgtaaatg tctcggaatt
     7981 aaaagcgatt ccaggagtac ttggcgtagt agaggatgaa cagctccaag tcatcatcgg
     8041 acctgggaaa gtgaacaaag tagcgcagga aatggttgac ttagcaggcg tgaaattagg
     8101 agaaaaactt ccttctgcta gtgcaactac agcaagttct tctgcatctg gaaaagaccg
     8161 tgtaaacgaa cgggcacaag aaatgaaagc ggcacaaaaa tcgaaacaaa aaccctctaa
     8221 aatcaaatcg gtattaaaag acatctctaa tatctttgta ccaatgatcc ctgcatttgt
     8281 cggcactggg atcgtggcag ggattgccgc agttttagca aacttagtca cagctggaac
     8341 actagacgca ggaacatggc aacaatatat cgatgtgatg aacatcttga aaaacgggat
     8401 gtttagctat ctagttatct atacaggtat caatgctgca caagtttttg gtgcaacgcc
     8461 aacgcttggt ggagttatcg gggctgttgt gatgctgaca gggatgaatc cagaagcacc
     8521 actgaaaaac ttgtttaccg gtgaagctct tgcaactgga caaggcggga tcttaggtgt
     8581 tatcttcgca gtttggttat tgtcaatcgt tgagaaaaaa ctgcataaag tagtacctga
     8641 tgcggtagat atcatcgtta cgccaactct ttccctgatt gccatcggtc tgatcgaaat
     8701 tttcttcatc atgcctttag cagggttcat ttctgacggc atggtcggtg ggatcaactg
     8761 ggtacttggt gttggaggag catttgccgg cttcgttcta ggtacattat tcttgccaat
     8821 ggtcatgttc ggactacatc agatcttgac gccaatccat gtacaaatga ttgatgaaac
     8881 tggtcgtacc ttattgcttc caatccttgc gatggccgga gcaggacaag tgggtgcagc
     8941 attggcatta tggatccgct gcaaaaaaga caaagaatta acagaaatga tcaaaggggc
     9001 tttaccagtc ggaatcttag gaatcggcga accattgatt tacggagtaa cattacctct
     9061 aggacgtcca tttattactg cttgtatcgg tggaggtatc ggtggggcag tgatcggaat
     9121 gatcggaaat gtcggagcaa tcgctattgg accttcagga gccgctttga ttccattgat
     9181 ctctgatggt aaatggttag gctatatcct tggtttattg gctgcttacg ctggtggctt
     9241 cgttgctaca ttcttcttcg gtattcctaa agaccgttta gaaaaagaag aactagcgac
     9301 cgaaacagcc acagtaaatg aagtacctga aaaagcagta cctcaagctt cagctgaaag
     9361 tgtgactatc ttatcatcag ttgctgacgg aacagttgtt tctttagata aagcaagtga
     9421 cccagttttt gcacaaaaaa tgatgggtga aggttacttt gtagaaccag aaaacggaca
     9481 tatctattcc ccggttactg gagtaataag ttctgttttc ccaacaaaac atgctatcgg
     9541 cattacgaca ccaaatggct tagaaatctt gcttcacatg ggcatcaaca cagttgactt
     9601 aggcggtacg ccattcgatg tgaaagtaac agaaggacag caagtgacac ctgatacgct
     9661 tgtagcagaa gctgacttag aagcaatcaa agcagcagac aaagaaacct ccatgatcgt
     9721 cttggtaacc aatatggatc gtgtaaaaaa ctttgtcttg gaaaaaacag gtaaagcgaa
     9781 agcaaaagca cctgtgatgg atgtcaaagc ttaataagaa tagaaacaat agagaacata
     9841 aaaagaagaa gccgaaattt atttttcagc ttcttctttt gtgttattta tttttttctg
     9901 ccaaaatttg ataaacgcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
     9961 agaaaggaaa gccgaatctt tgtgcgtatt gtttggctgt cagacaatat tgattgttga
    10021 agtttttatt accgctaccg actacaccaa ggcaaagttt atgattgtct tcgtattcca
    10081 gatattcgcg catcgtttcg gtcaagattt ctgtgtcacc gttgtctaac ccattcccgc
    10141 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    10201 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    10261 cagcatattc tgctagatgt ttggcaaaag cacgcgtatt tcctgaaatc gatatatata
    10321 aaatgttcat ggatcatccc ccaattcttt ccctttagta tagcatcttt ttaagttttt
    10381 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagtcgg
    10441 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    10501 actttttgat aaaagcggtt ttactttgga ggatgcttac atggatacag agcaacagaa
    10561 aaaaggaatt ggtttgattc cacttgctgc cttagtgatt ggttcagcga ttggaggtgg
    10621 cgtatttggt attatgacgg acatctccgg atcggctggt ggcccagcta tcttcagctg
    10681 ggtactcgta ggaacagcaa tgttgatgct ctccttatcg atcaataata tcaataaaaa
    10741 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    10801 tggttttatc agcggttggg gttactggct cacatgctgg ttaggaaacg tggcgttcgc
    10861 aacattgttg atgagtgcat taggttattt cttccccgta ttcgaaggtg gtcaaaatgt
    10921 ggcatcgatc gtgatgagta cgatcttctt atggggatat gcatggctag tgaatcgtgg
    10981 agttgaaaat gctagctttg tcaatgctat cgtcacattt tgtaaattag tgccattatt
    11041 tttatttatt gtagtagcta tcagcacctt ttcgatccaa caattcatta ataacttttc
    11101 tagcgatatc atattgaatg cgaatgggca gccgatcccg ttttctagtc aagtattgag
    11161 ttgcatcatg gtgatgctct gggtattcgt tggtattgaa ggagcttctg tcgtaggttc
    11221 acgagcaaaa aaacaatcgg atgttgggaa agcaacgatt ctaggaatat tcggtttgat
    11281 cattatttac gtcttggttt ccatgcttcc atatggcaca atgacgatga atcagctgca
    11341 aacgatcaaa gcgcaacctg ctatgggata tgtatttgaa atgatggttg gtaaatgggg
    11401 ggcggtcgtg atcaacggcg gtttgatcat ttctttagtt ggggtttggc tctcatggac
    11461 aatcttgccg attgaaacaa tggggaatat ggctgaggac ggacttttgc ctaaagcatg
    11521 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    11581 gaatgtcttt ttactttcat tattattcac tgacagtgca tataactttg cttatacact
    11641 tggtacagca gcgattttct tcacgtggct tttcttaggg ctgtaccaaa tgaagctttc
    11701 ttatcagcgg aaagagtggg tccaattttt agtagggttt ttagcaagtg cttttcaagt
    11761 atgggccatg ctagttgtag cctttaatga agtgatgctt gttcttgttc ttttcttacc
    11821 aggtatttat ttttacctca aagcacgtaa agaacaaaac gaacctgtac aacatatcca
    11881 cttagataag attttactta tcggtgtgac gatggtcgga gtaatcgcaa tggttttatt
    11941 tgccactggg atgcttcacg tatagaagta taaagagagg ctgtgaagcc tgtggtttat
    12001 tcaaaaa
//
LOCUS       DAZKSU010000080        11731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000080 DAZKSU010000000
VERSION     DAZKSU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.081"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            395..601
                     /locus_tag="ACG8Z3_002283"
     CDS             395..601
                     /locus_tag="ACG8Z3_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0824570.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(803..1804)
                     /locus_tag="ACG8Z3_002284"
     CDS             complement(803..1804)
                     /locus_tag="ACG8Z3_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0824571.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1809..3722)
                     /locus_tag="ACG8Z3_002285"
     CDS             complement(1809..3722)
                     /locus_tag="ACG8Z3_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0824572.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3890..4396)
                     /locus_tag="ACG8Z3_002286"
     CDS             complement(3890..4396)
                     /locus_tag="ACG8Z3_002286"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0824573.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4556..4996
                     /locus_tag="ACG8Z3_002287"
     CDS             4556..4996
                     /locus_tag="ACG8Z3_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0824574.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(5022..6179)
                     /locus_tag="ACG8Z3_002288"
     CDS             complement(5022..6179)
                     /locus_tag="ACG8Z3_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824575.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6182..6538)
                     /locus_tag="ACG8Z3_002289"
     CDS             complement(6182..6538)
                     /locus_tag="ACG8Z3_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824576.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6836..7810)
                     /gene="sgrA"
                     /locus_tag="ACG8Z3_002290"
     CDS             complement(6836..7810)
                     /gene="sgrA"
                     /locus_tag="ACG8Z3_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0824577.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(8006..8845)
                     /locus_tag="ACG8Z3_002291"
     CDS             complement(8006..8845)
                     /locus_tag="ACG8Z3_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0824578.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            9030..9917
                     /locus_tag="ACG8Z3_002292"
     CDS             9030..9917
                     /locus_tag="ACG8Z3_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0824579.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNNDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10511..11206)
                     /locus_tag="ACG8Z3_002293"
     CDS             complement(10511..11206)
                     /locus_tag="ACG8Z3_002293"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0824580.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(11190..11588)
                     /locus_tag="ACG8Z3_002294"
     CDS             complement(11190..11588)
                     /locus_tag="ACG8Z3_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0824581.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3501 a         2323 c         1756 g         4151 t
ORIGIN      
        1 tctaaaaaat taaaaaaaat tttggtgaat taagtatttc cttgataaca ctggcttttg
       61 gcctcatttt aggttatcat ttgtaatttt atttcacagt tgatatgatt gttatgtaaa
      121 ataaaaaagt ttaggataca tcgaattttt ggggaaattc ttttgggggt atcctaaatc
      181 ttttctatta tattcccata ttttctatta ttggtctaga cgtatgatgg taaactttgt
      241 acctatctga ttgctgaatc attaattgct tatcattagc catctttgtt tcaagttcgc
      301 tagatgatca ttttattcac atcctaacaa acttgtcaca tttatagatt cttaatctat
      361 ttatcataca attataaaaa aggaggtgct gcttatgaaa gctatccata tttctttgct
      421 ttttttcgct agtgcattga ttttatttgc tattcatctt tgttcccagc cttatatcaa
      481 taacaacgga atattagtgg aaccagcatt cttttgctta ccactcggct ttttgtccct
      541 tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa cgatcaaaac aacaaatctg
      601 acttataaaa tttacgtaaa agagatcgtg acaataatcg tgtgatattc ccaaatttcg
      661 aataaaaggt gccccttgcc atctctaact cctcagtcac aagttacaat attcataaac
      721 catgaaaagc ttgtttattg aaattgctcc ttgtgacttg gagctaagtg acagattaca
      781 acctcttttg catcataaga ctttactcct tgaaaaacat tactaacttt tgatatgttt
      841 cttgcgcttg tttattgctg aaatcaaact gatattcatg gttcaaaaca gcatttttct
      901 ccgcataatc tacttttgtg actttcaccc ccgctttctc aaatacagaa gttacttctt
      961 ccatttgttt cgtaaacgta tttgtatttc catccgttaa gaatgtgttt tgcgtccaac
     1021 gatccgcatg tctgaccaag gctaaacttt tgaccttctc tgattgttca aagtccttat
     1081 tatcaaagta agcccatcca catttttcat aaagaaagct agagatttta tcacttactt
     1141 gagtaagtgc agaaggatcc aacagaccag acaaagaaat aaacttcgta atctgcttgt
     1201 tctccaaagt cggatcaaga tgatcagctt ttttaatttc ttcattttga agtgctaaca
     1261 aatattctcc actaatttgt gcacctgccg aatttccacc aatcgaaata ttcgaccaat
     1321 ctgcttgatc ttttatctct tttttagctg cttgaatggc ttcgttcaat tggataactg
     1381 gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat atttacgatg atttgctgcg
     1441 tgtctgaaca tagatattcc aaataatcac gacaatcatt tttatcccct cctacatatg
     1501 cgcctccatg catccagaaa agaatcggta attttttctt ttccactttc ggtgtgtaga
     1561 tatccatatt attatttttg tactttgaag gataggatat ttcttttttt atatcgattt
     1621 gttcgatttt ttgctggaaa tcatctggtc gggcatatac ttgttctttt tcacttgtgt
     1681 caaattgttt tctaacaagc aagctaatcg gctttggtgt gatttgatta atcaaaaata
     1741 cagtgactaa aataactacg atgataactg gtactgcaat cactattcgc ttgaaccatt
     1801 tcattcattt accctctctt tcttcgccaa atccatgtga taatcgtgct aacagataaa
     1861 aatccacctg aataaatgag aagcgaatgt ttcttttctc cagttttcgg caacattctt
     1921 ttttttgcat ctgtcttttt ttgtactttc actgtcatct gaatcggttg cgtatcatca
     1981 cttgattgat cctctcgagg ttcattcttt cccttattgt ctgcattctc tgtcggatct
     2041 tgcttttttg gtggagctac tgggattttt ttgtaataga aaataatagt tggtatcgtc
     2101 tcattaaaaa tcccttcagt cggtccttca acatttgcta attcgtatcc ttcgaatgtc
     2161 ttttgctctg tcatataatt ttctccataa gctccgtaaa gctggatttc gtcctggtct
     2221 ttgagtagac ttttattgtt ttcatccaca tactttacgt ttatctgatc atggaacact
     2281 actttcaaaa agtccgttac atgtttcgtg taggcagcat ggtcaacttc ctgtgccttt
     2341 ccatgccctg ctccttctac taataaaaga tctttatacc ctcctgataa ctcatataat
     2401 ttttgcgcgt tagacacagg tactacatca tctgctgttc catggataat caatttaggc
     2461 aatgaatctg acattccaat cgtaggtaga gcagcttttg tatccaaatg catctcctga
     2521 tcaaaatagt attgtttcaa aaggcccata aataaatctt catgctcttg atccccaatg
     2581 atgtctaact gattttttat ggcatccggt agtgcggggg ctactgtctg gctgtacgta
     2641 aaacgtagtt gttcatcaat tgatgcaaat ccgcaatttt caataattcc tttgacattg
     2701 acaggaatat ctttcgctaa tacagacatc atcgtagctg ctcccatcga accaccataa
     2761 agtaaaatct tttgagatgg ccgctctcga acttcttgat ttatccagta aaggacgtca
     2821 tctgactcat attgtccata ggtcacataa tcgcctccgc tattaccagt cgctctggaa
     2881 tcaacaaata ataaattata cccggcctta taaaaatcat tgtattcttc tgtaacgatc
     2941 ccgttattcc aattccctcg aaatccgcca tggatcatga ctgttttatc tgaaccttga
     3001 tcgacataat atgcatatac atccatcatt ttgccatctt gtttgtttag tacagtttga
     3061 tgaagttgtt ttgtatttgg atagaagcct gtgttttctg taggcgttgt cccatcatct
     3121 gtacctccaa gcatcgacat caacacactg ttttttgcaa aagcgatatc gtacaataga
     3181 taagtagcaa tcagatgttt gataatctct ttatctacac cataatcatt aacagcaagc
     3241 tcagtgacta ataactctat taattttgac ttgatgtctt ctgttggtcc attaagtatt
     3301 tctgtcatct gtttaccgaa gtaattcaaa tattctttac cattgaaatt ttcaccttta
     3361 ccgatatcat cattttctgt cccattctct ttgtcactta ctttagcaga agaggattcg
     3421 tcgtttgaca tttttgtagc tgttgcttct tctgtttgtt ggttatctgt ggtagcggtc
     3481 gtttcttgat ctgtgctagt aacagatgtc tcaaccgttg tttcagtatt tgtttctgtt
     3541 tgtgaagtac tcgatttcgt ttcatttcca gcatcagtag tttctgtggt ttctacctcc
     3601 cgagattccg attcggaaga aatgctctct ccatatactg tccattgcgg gacgcttgta
     3661 cttacagcaa ataagatgac caacgctccc tttaatactt gttttgttct caagctattc
     3721 actccttttt atgtttctag gagaattata tgtaaacgca aacatttttt atacaaaaaa
     3781 taaatggttt atcccataaa aatagaacag gattattttc tcattttacg taataaagca
     3841 gaatggataa accagtatcc attctgcttt ttttaaacga agaaacaact tattgctcca
     3901 aaaatgtcag aaatcgttta tcccaaattt tctcgtaaaa agcgattgtt tcctttgctt
     3961 tcataaaagg attatcataa gttgtcgttg ctcctctaca catcataagc tggtatccta
     4021 atccatgagc aaacttcaca gtcgtatcta tacaatactc tgcttgtgcc ccacataatt
     4081 cgatcgaatg acagtccaat tcagttaata aaacactcaa attagtctta taaaatgaat
     4141 tcgcatgggt ttttctcaca aaataactgt gctcaggaat agataattcc gacaatagct
     4201 cccatggttt tgtttgtggc actaattgag catcttcgtg ttgaacgaaa ataactggtt
     4261 tcccaatcgt ttcgtatgtt ttgattcttt tattcactaa tgctgtcact gtttctaatt
     4321 cgtgtatcgg cgaagaatca aaacataccc ctttttgtaa atcgatcact actattgcgt
     4381 cggctgtgtt tgacatactt gtgccttctt tcttttttct tgatttaata gtatttctta
     4441 tctagctagt tccattatac gtgacaacat tttcttattt tacacctcta aatggcactt
     4501 ttcgactatt tcttcatgtt attatttcat aaatacttag ctattggagg aatgtatgat
     4561 gattaaattg tatacaggtt ctggctgctc gtcttcaaga cgcgcaaaac gttggcttat
     4621 cgtccatgat ataccgttta tcgaacagaa cttaattcgt caaccactaa ccaaaaatga
     4681 atttatccac ttactaaaat tgacaaacaa cggcgtgacc gaaatgctct ctactcattc
     4741 tattagttat aaaaatcttg ttcacagaat agatgaactc agtttgagtg aactatatat
     4801 tgagattcgt acatcccctt ctttattgaa aaaaccgatt atctacgacc aatctgcaaa
     4861 acttttgact ggatttagcg aagataaaat caggatgttc ctttctaaaa ctaaaagaaa
     4921 gcaagagctc aacgaactct tgctttctgg tcatcttgtc gattttaaag aaaataatct
     4981 ttttgcaatg tcttaatttt ggcataaaca atctgctttt cttaattaac gaaatccaat
     5041 ttttccaata tggatctatt tttctcattc ctgattttta tgatttcagc tttcagacgc
     5101 gcccaatcat tttttgtagg ttgtccattc cagtaaaata tctgtgtatt cgtttgatca
     5161 tatgcctttt tgtcgatagg ataatcgcta atgacaatat caggtaactg atcagcggat
     5221 gcactatatt ttattgggcg ttcagtcata gataatatcc ttttctttgc ccataattcc
     5281 tgtgttttac catactttga ttgaatatag aatatgacag gttgttcgtg ttttataaaa
     5341 atatccctga ctaacataca gtattgaaaa atcaaccgtc tgttcttttc ataataagta
     5401 ttaagctctg gaattctgcg agctacccca ctaaaaaact gttgcatgat tggatacatc
     5461 actggaaata tatgctgaaa atctctgtct tctgctgatt ttccaaaagc atccaccttc
     5521 ttttttgtcc caaaaaccct tgaatacaaa tggatcattg ccaaattgac atatagataa
     5581 tttttttctg aagttgtaaa acaaatcttg aagttttttt caaaattttc tacaatagac
     5641 gattcaatcc agccccaact ttcttgtact ttatctggag tatccaacaa atcttcttcc
     5701 aaataggtgt tcattacacc aaacataaaa taaaggtaag cttgctctgt tggagtgatg
     5761 tgattcatcg accagaagtt tgcttcaaaa aaaggctcta gtatgtaatg cgtaaactct
     5821 tcttttgtta tcaatggatg cacaaatttg ttcatctctt ctgggacata cgtaatcgag
     5881 tggccttgtt tcattcgttg caacgaaatt cttagtaggc tgatccattt ctttctatct
     5941 atactacgcg tatatggcgc atgtttcgca aatttttcta atagttgacg tttatttttt
     6001 atacttgata atatatcaga atgattttcg tcgaatgact gccaatacat caagtgaaaa
     6061 aagtatcgga tacggtattc ttctccttct atcggattac cttttttcaa gctgcaagat
     6121 aaatgatagt ttttcaaaat agtattaagt tctcttaatt ttctttgaat cgtacgcaaa
     6181 cttatataat tttttgacta agattttcta aggattgata agtacctttg taaagtgaat
     6241 ccagtaaaat ataaccaatc gttccctcta aaagtataga ataaacttta ctcagtaaaa
     6301 actgagaaga aaactcgact tttatcatct tgttttctga aagaaaagat aatttatatt
     6361 ctggatacct ttcgcaaaac gattctgctt gttgaatcac cgaattcatc ttagccaggg
     6421 tgtagtttag atctaaagcg atatccttca ccacatataa tttctcgtct ctttcataac
     6481 aatagttcaa aaattgatat acttgccttt cattgatatc aataaattct tcaaacaaag
     6541 tacatactcc tttttatcta ttatttagac ttattccgac agctacatga ataacgtaca
     6601 aatagattcg ttactgattt ttcagagata ctaccatgcg cagtgtgcat tgtcaattat
     6661 tcttgctctt attttccatt tgtgttcctg ttatttcctg tttctcaaat atgttagtaa
     6721 acaaaaatag atctggcaaa gctacaactt cgccaggcaa tacagcactc tcgaatgaaa
     6781 tattcactca aaaatctatc gttttattca attttagatc tgttgctttt attctttatt
     6841 tcactcgatg gaagagagaa cgaaaatagc ttgctcctac aatcaaaatg agtaacacac
     6901 ccatccatgt aattagcttg ttcaaggttc tactaccagt ttctggtaaa tcgctttttc
     6961 tattatctgt ttctttcgct ttttcttgat gatcaattcc acgagaaaca aaatcaggct
     7021 gttttagcac actttttagg ttgctccctg catttctctt ttgttcctta gttggtatga
     7081 taggtaccaa atcaagttca ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc
     7141 ctgtgctact ctcactactc atgcttcctg tgctactttc actactcgta ctctccgtac
     7201 tgctttcgct gctcgtgctt cctgtgctac tttcactact cgtactctcc gtactgcttt
     7261 cgctgctcgt gttttctgtg ctactttcac tgctcgtact ctctgtactg ctttcaccgc
     7321 tcgtgttttc tgtactgctt tcactacttg tgctatccgt actgcttggc ggtccaacaa
     7381 ctgtccaact gcttggtttc aaattctccg tgatcgtcat ctgtccatca taaaaaacaa
     7441 tcaaatcaac tttatcttta ctcttgatct catcaatcga tagttctgca gaaatcatta
     7501 ttgtatactc aataccagcg gtccattgta cgtcttttaa ataaacttcc ccaataactt
     7561 tttctgaact tccattacca tcccaaccat atgttagaac agcatgtaca tcactagcat
     7621 tcacgctttt gtttggtttg actggaatag taatgacttg ttgtgtcatg tcataaacaa
     7681 tccgatcagt tttcacagta aatgaaagat tctcatttgc ccgttcattt gcagaagtac
     7741 tgattccaat cattgctgca attatactca gcccaagaat aacaaataat acggtcgctg
     7801 ttttcttcat ttctatcact ctccatataa ttaccttcta tttgataaag gaattatata
     7861 aaatgaaaat gatgagaaca cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta
     7921 ttcgcttgaa tttgtttact ttttcgttta atcaatgaca gacttaaaaa agcaagaccg
     7981 atatcagcct tgctttcttc atcttttatt gataaacagc agtgtatttc ttcatccgct
     8041 ttgttttata tgcatagagt aacaaaccaa caatcaagac taacaagaat aatgtagttg
     8101 atatagatgc ttcagcccca aagtttcccc cagtaattat tgtattttgc gattggatca
     8161 gagtggagaa aaaaccttgt tcttgcgttc cgctgactgg tatacccaaa atagctcctt
     8221 gtgtccaatt ccaagtaata tgagcaccaa tagccgtaaa taaacttcct gtactgtaga
     8281 taagtgcgct gaaaaagcaa cctgctaaaa agatattgaa aactgccaga aatgtcatgt
     8341 taggattcaa tccgtgcaaa agagcaaata atgcagcaga tccaatcatt gctattaacg
     8401 gaccaaatct aagcatctga tgctgaaaaa agccacggaa taaaagctct tctgatgccc
     8461 ccactgcaat aaataaaata aaactcgtta tcagcggcac aatgtttgcg tttcctgaat
     8521 ctaaccgata cgcccccgtc aaatataatg gactagtaat aagcagaaac actccaattc
     8581 caacaacgag acctcccgct aaataccccc agtcttttcc tcgaacagaa acaccataat
     8641 cttctattct ttttccttcg accttactag caagaaataa agctagtaaa aaaacagcta
     8701 aatgtccgac cgtaccggca attgctccaa tccattcact atttccaata aacatatccg
     8761 caagtgcatt attgacaaga attcctaaaa caaaaccaac taagactatt aaaacagatc
     8821 caatcaaccg aataatcctg atcatctttc atccctcctt tatattaagt ttaacaaccc
     8881 ttttctttct tgtctcgtga aaactatgta aatgttctcc atcatagaga gaatagattt
     8941 ttacgaacta agaatatcta cagcaacctt acgaaaatga aagactgtct ccctaattct
     9001 tctgttagac tgtttttaat aaggaaagga tgggaaattt tttgaaaaaa caactactaa
     9061 ttatgtgcct ttcagcatgt gcaattttac tattctcagg ttgtagcaag tccaatgaaa
     9121 cgattgcaac gtttaaaggt ggaacactta cagttcagga tttttatgat aacccaaaag
     9181 ttaaatcaaa taatcaaaca ctattaaaaa aaatgattgt cttcaaagca tttgatgatg
     9241 tatacggaaa agagatttct tcaaaggaag taacaaaaga atataatgaa caaatcaaaa
     9301 aactcggtcc aaactacaag gaacaattaa aagcagttgg ccaaacagaa gagacatata
     9361 agaaactctt taaacagatg ctggctttcc aatacggttt gaaagccaat gtaaaattaa
     9421 cagataaaga tctagacact gcttggaaag aattctatcc agaagttagt acacagatca
     9481 ttctattttc gactaaagaa gaagcagata aagcaaaaaa agaagcaaat gaaggagaaa
     9541 atttctccaa gcttgttcaa gcttatggaa aaaataaaac attaaaggaa actgatggaa
     9601 aaatgaattt tgattcaacc aatccagaga ttcctactga agtaaaaaaa gcagcattta
     9661 aactaaaaaa cggggaagtt tctgatatca ttcctgtaac agatccaacg acttatcagc
     9721 aaagctatta tctagtaaaa atggtaaaaa aacaggataa aggttctaat aatgacaaat
     9781 acaaatctga gctagaaaaa attgctaccg aagcaaaaac tcttgatacg gaattcatgg
     9841 ataaaacaat ccgtaaagtg atgaaaaaag acaatgtaac tattaaagat ccttatgtaa
     9901 aaaatatatt caaataaaca aatttgaccc aattggggat gtggcaaaat ttgagaagtg
     9961 atttttggaa attccttctt atttttgagg caaaccactt ttgccacaac ttcatcgggg
    10021 ccttttttta tttgtaccaa aacaaatcac tggatttgtt acaatcatga ggatataatt
    10081 taaaaaagtt ttttatttct atcacatgaa tcaatgcgcc attaagtaga ttctttcatg
    10141 cttccctatt ttttcacttg ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg
    10201 actgaaaagt tattttaaaa aagactaaaa atatcccttc tggacttgtt cttcttattc
    10261 tgttatacta gagaaacacg aaagatattt ttatgcgaca cttttcattt gtttttatga
    10321 aatttattca aaactggcgt aacttcagag ttacaaggat gtattagagg ttagggaata
    10381 catcgtttaa atgagaaata agtaatagtt agtaatagtc acccaaactt taaattaatg
    10441 aatattgaat cgtcagatag cctcctcgag ttttcgagaa ggctatcgtt tattttatgg
    10501 agaaattgat tcagaagtaa tcagcgacct tttttagttc accaatcgtt tctgcttgga
    10561 aagtttgcac ccaatcttct tggaagcccc tcactgcttt atcaatcgag ggcatctcta
    10621 atacttgatc tagtagctct ttacctaacg ttatcgcatg agctccagat cgaatggctg
    10681 tagtgacttg ttcacaattt ttgaaactag ctgctaatat ttttgttggt ctttgcgtac
    10741 gttcgatctc agtccgcaac gaatcaatca ctttcatagc atcaatgcct tgattttgca
    10801 tcctgttata ataaggtgca atataatctg ctccattatt aatagctaaa tacccttgta
    10861 attctgtgta aatagctgta ccggtaatca aataaccttc ttttttcaac tttttgatcg
    10921 cttgaaggcc tgcttgattc accggcacct tgataaacgt atcttttccc agttcttcta
    10981 tgataactcg agcatcctct atcatttctt cttccgttct tcctattgtc tgaatatgga
    11041 taggaatctg tccgattatt tttttgattt ctttcatatg cgagaagaag tttgacgctc
    11101 cttctttttt cagaattgta ggatttgttg ttacgcctgt taaattcaaa aactgttgac
    11161 aagaataaat actttttgta tttgctgtat caagcatgat ttccatatag ctttccccca
    11221 ttctgatcct cttgatcgat tttaatgatt gtcccctttc ttaaatgcgg catttcctta
    11281 tcttctacat acattgttcc tggaagttcc ggctcatgag atccatcaaa ttttatcgtg
    11341 atatgtccta attcattcaa attagtttgg acaacatctc ctactgctgt gattacatac
    11401 atttgtttat caaaacagag cttcatatca caagttattt cttttcttac ctgattcaat
    11461 tcaatcttat aacaataatc tgttaatgtt tccggtgctt catctccaaa taaaatcatt
    11521 attttttctt tcctgaatac ttctgcttct ataccaacat ctgttacaac cgtttcaaag
    11581 atcgtcatct ctgttcctcc tcgtaaaata tcatttgtct aatagttttt attcaaaatg
    11641 tttctagatt ataaaattta ttgaataaca cattcctaac ggaatttgtt tcgatattgt
    11701 gtgatttctc agagtacaat ggatgtatgg a
//
LOCUS       DAZKSU010000081        11568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000081 DAZKSU010000000
VERSION     DAZKSU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.082"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(264..749)
                     /locus_tag="ACG8Z3_002295"
     CDS             complement(264..749)
                     /locus_tag="ACG8Z3_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0824582.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    839..1184
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1229..1870
                     /locus_tag="ACG8Z3_002296"
     CDS             1229..1870
                     /locus_tag="ACG8Z3_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0824583.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1889..2524
                     /locus_tag="ACG8Z3_002297"
     CDS             1889..2524
                     /locus_tag="ACG8Z3_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824584.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2542..3357
                     /locus_tag="ACG8Z3_002298"
     CDS             2542..3357
                     /locus_tag="ACG8Z3_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0824585.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3676..5112
                     /locus_tag="ACG8Z3_002299"
     CDS             3676..5112
                     /locus_tag="ACG8Z3_002299"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0824586.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5301..7748
                     /locus_tag="ACG8Z3_002300"
     CDS             5301..7748
                     /locus_tag="ACG8Z3_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0824587.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7978..9045
                     /gene="rlmN"
                     /locus_tag="ACG8Z3_002301"
     CDS             7978..9045
                     /gene="rlmN"
                     /locus_tag="ACG8Z3_002301"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0824588.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9035..9241
                     /locus_tag="ACG8Z3_002302"
     CDS             9035..9241
                     /locus_tag="ACG8Z3_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824589.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9238..10341)
                     /locus_tag="ACG8Z3_002303"
     CDS             complement(9238..10341)
                     /locus_tag="ACG8Z3_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0824590.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10894..11442
                     /gene="aac(6')"
                     /locus_tag="ACG8Z3_002304"
     CDS             10894..11442
                     /gene="aac(6')"
                     /locus_tag="ACG8Z3_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0824591.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3846 a         1950 c         2489 g         3283 t
ORIGIN      
        1 taatattttt tgttaaatat gactgaaatc aaaagaataa aattccgatt tataatcgaa
       61 aaaagaagca tttttatata tgcttctttt ttcgtagaat caataaatat ggatttggct
      121 aactatggaa gaatactcgt attagttttc agcgttcata ctagttgtta cattgtttga
      181 ttggccaaaa gaatttctga tatattttgt gctaaataaa gaaaaagacg cttgaattgg
      241 gaaataagaa aactactcta tcattacaaa ttttcataaa ctggcagatg ttctaagtaa
      301 acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg tagtggtatt gatatttgga
      361 tgaaagccta cggtatcttg acgatcaata gcagcatcga tcacgacttg gatttttttc
      421 tctttgtcgt gcatcaatgc taatggagtc actgtacctg ctggaacgcc taggaggtca
      481 ggaagctgtt cttcagaaac aaaagataag cgcttttctt ttaattgttc agctagttgt
      541 tttaaatcgg cgtgcttttc atcaggcaag atgacaagat agatttgttt tccttttctt
      601 gatttcaata atagattttt tacttgcgga ccaggtaggg aaaaagggac atttttgact
      661 gacgtaatcg caggatgatc gactttttga aaagagatac ccagtttttt tagtagttca
      721 taagcttctt gttcggttgc acgactcatc gcgcatcacc ctcctttttt tattagtgta
      781 tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca
      841 aatgacatcg aaaagaagag tagccgttag gtgtttttag agagtctctg gttggtggaa
      901 agaggcagca gttaaccgtg aaacacatct tggagtcgat tttctgaagc gtatagtagg
      961 aaaatccggg gagcccgtta cagctgcggt cgttgctaga tgaccagata aggctggctt
     1021 ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat taacgtcctt tgatcttcgg
     1081 atcaaaggac tttttgtttt catctgaacg gcaacgactg tgtgaggttt tttctgcaag
     1141 gaaaaaataa acagaggtgg aaccacgtat aatacgtcct cttggcattt gccaagagga
     1201 tttttttatt ttaaaggagg aaaagaagat ggagtatttg attgaagtta tgccgcaatt
     1261 attagtagga gcactcacaa cattgaaatt atttttattt accttgattg gctcattacc
     1321 attagggatt gttttagcat taggattgtc tttgcatttt aagccgctac gctacttgct
     1381 tcaaacatat gtatgggtta tgcggggaac accgctgttg ttgcaactga tttttatttt
     1441 ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt
     1501 tatcttaaac tatgcagctt attttgcaga gatctttcgt ggagggatac aatcgattcc
     1561 tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa
     1621 aatcgtcatc ccacaagtga caaagattgt tttaccttct gtagggaatg aagttatcaa
     1681 cttggtcaaa gattcttcct tgatctatat attaggttta ggtgacgtaa tgcgtgctgg
     1741 gaaaatcgcg atggaacgcg acgcgacttt actgcctttg ctaggcgtgg caatcatcta
     1801 tcttacattg actggtattc taacaatcgt aatgaaacaa gttgaaggaa aattaaatta
     1861 ttaccgctaa ctgataggag gaagaaatat gattgaattg aagaaagtga acaaagcatt
     1921 tggtgaaaaa aaaataatca aagatttgaa tctaatcatt ccagatggga aaacattagc
     1981 tatcgtaggt ccttccggtg gcggtaaaac cacgctttta cgtatcttgg ctggcttaga
     2041 aagttctgac agcggagaat tttatttaga cggtgtaccg ttcaacccat tcgagcttaa
     2101 aatgaaagaa caagttgtag gagtcgtatt ccaagacttc cagctatttc cacatctgaa
     2161 tatttttgaa aacattacct tagcaccaaa aatggtattg aaacagacaa aagaagaata
     2221 caccaaaaaa gcagaagaat tagcggcttc attagggatt agtgatttat tgaatatcta
     2281 tccttatcag ctttctggcg gacaaaaaca gcgggtagcg attgccagag cattggctat
     2341 gaatccacgg gtattggctt atgatgaacc aacaagtgct ttagatcctg aattacgcca
     2401 gcaagtcgag cgtttgatcc tatcattgaa agaagatggg gtaacccaaa ttattgtcac
     2461 ccatgattta gtatttgcag aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca
     2521 ttaagaaagg agaagataga gatgaaaaaa accagttttc tattcgcttt gatcgcaggt
     2581 cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag cagataattg ggcgactatc
     2641 aacgaagaga aaaaaatcgt tataggttta gacgatacat ttgttcctat gggatttaga
     2701 gataaagacg ggaagctcac aggatttgat attgatttag ccaaagcagt ctttgaacaa
     2761 tatgggatta cggtagactt tcagcccatt gactggtcaa tgaaggaatt tgaactgaac
     2821 aatggaacga tcgatttgat atggaacggc tattccaaaa cgccggcaag agaaaagaaa
     2881 gtgcaattta caaaacctta tatggaaaac gatcaagtgc tgatcacacc taaagaatca
     2941 ggaatcacaa actttggaca aatgaataat aagagattag gtgcgcaaaa tgggtcatct
     3001 ggttatgatg tatttacgaa acaaccagaa gtattaaaag agatagtcaa aaaccaagat
     3061 gctgttttgt atgatagttt taatgaagca ttgatcgatc tgaaaagcag acgggtcgat
     3121 ggtctgctga tggataaagt atacgcagac tattatttga aacaaagaaa cgaactagaa
     3181 gacttcaaca taacaaaagg ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag
     3241 tcagatagta cattagtgaa aaaaatcaat gaagccctag atcaactaga gaaaacaggt
     3301 gaattccaaa aaatatccga taaatggttt ggcgaagatg ttacgccgag aaattaacaa
     3361 ataggggact gtgacaaaag acgtgtcaca gtcccttttt ccgaataaac agtgttctcc
     3421 tgccaactcc ttgtgactcg gagctgaatg gcaggctcac aacctcttgg taaacggtgt
     3481 tcaaaagcca gtttttcggt attaagccaa atttatgcaa aaatgtagaa agccattttc
     3541 acaaaatttt tcttactact cgaaactaag cgactttttc acaacctcct ttacataatt
     3601 ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga ggaacgattg aaggttatag
     3661 gaaaggaaga gatgaatgga acacaaagaa cttacctcat ttccaaaaga ttttctgtgg
     3721 ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa
     3781 tcagtctggg atcgatttgt acggatacct ggaaagacat ttaaaggaac gaacggtgat
     3841 ttggcagtag accactatca tcgttataaa gaagatattg ctttaatgaa agaacaaggt
     3901 ctgaaagctt accgcttctc tgtagcttgg acacggattt ttcctaatgg acgaggagaa
     3961 gtcaatcaag ctgggctcgc tttttatgag agattgatcg acgaattgat tgaaaacgat
     4021 attgaaccta tgttgacttt atatcattgg gatcttccac aagcattgca agatgaatat
     4081 aatggttggg aatcacgtca gatcattgaa gatttcactc attatgcaga aacattattt
     4141 gaggcattcc gcgggaaagt ccattactgg attagtttga atgaacaaaa tatcttcact
     4201 tcattaggtt atttgttagc agcacatccg ccaggtgtga cagacccgaa acgaatgtat
     4261 gaagttaacc atattgctaa tttagcaaat gcatctgtca tcaataaatt tcacgagtta
     4321 gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc caaattatcc aattgacagt
     4381 aatcctgaaa atgttctggc tgctgaaaat gcagaagacc tgatggccaa ttattggatg
     4441 gatgtctata tgtggggaaa atatccgatt gctgccatga ggttcttaga agaaaaagga
     4501 tgggcaccaa cgattgaagc tggcgatgca gaattgctag aatctgctaa accagatttt
     4561 cttgggatca attactatca aacggcgacg aatgcattca atccattaaa cggagttggt
     4621 gctggaaaaa tgaatacgac aggaaaaaaa ggttcatcag aagaaactgg agttcctgga
     4681 atgtataaaa aagttgagaa tccatttgtc gaacgaacaa attgggactg ggagattgac
     4741 ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat gccggtactg
     4801 attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca aatccatgac
     4861 gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc aatttcggat
     4921 ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac
     4981 ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct
     5041 ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga
     5101 gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt
     5161 tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa
     5221 agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt
     5281 gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga
     5341 cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt
     5401 ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga
     5461 ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag
     5521 ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac
     5581 gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat
     5641 ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca
     5701 gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc
     5761 tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat
     5821 tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta
     5881 tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc
     5941 gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc
     6001 gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg
     6061 tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg
     6121 agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag
     6181 agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt
     6241 tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag
     6301 actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg
     6361 agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg
     6421 tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc
     6481 cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt
     6541 tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg
     6601 aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa
     6661 accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa
     6721 cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt
     6781 cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa
     6841 aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc
     6901 tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac
     6961 aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt
     7021 acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat
     7081 tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg
     7141 aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag
     7201 ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca
     7261 gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg
     7321 cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga
     7381 tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta
     7441 cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga
     7501 caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg
     7561 aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag
     7621 cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca
     7681 agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg
     7741 acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca
     7801 tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt
     7861 gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta
     7921 aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg
     7981 gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac
     8041 ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt
     8101 aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt
     8161 atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat
     8221 ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc
     8281 ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc
     8341 gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg
     8401 gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg
     8461 gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat
     8521 gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag tggattagca
     8581 cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac
     8641 gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag
     8701 aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa
     8761 tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta
     8821 ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa
     8881 catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag
     8941 aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc
     9001 ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt
     9061 tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa
     9121 gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa
     9181 gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta
     9241 actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag
     9301 aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt
     9361 tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt
     9421 taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa
     9481 ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta
     9541 tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt
     9601 atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac
     9661 ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata
     9721 agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc
     9781 tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat
     9841 acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg
     9901 tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac
     9961 acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt
    10021 agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa
    10081 caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt
    10141 tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt
    10201 tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc
    10261 atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg
    10321 taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt
    10381 ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga
    10441 taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga
    10501 acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac
    10561 cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga
    10621 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
    10681 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
    10741 gaataaagtg tttctgtata atttttttat tttataagta agataaataa tgaaagaata
    10801 gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac
    10861 tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat
    10921 aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat
    10981 ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca
    11041 gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca
    11101 ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact
    11161 cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta
    11221 ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca
    11281 tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa
    11341 ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt
    11401 tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca
    11461 gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt
    11521 gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtac
//
LOCUS       DAZKSU010000082        11247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000082 DAZKSU010000000
VERSION     DAZKSU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.083"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..2012)
                     /gene="metG"
                     /locus_tag="ACG8Z3_002305"
     CDS             complement(3..2012)
                     /gene="metG"
                     /locus_tag="ACG8Z3_002305"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0824592.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2280..2558
                     /locus_tag="ACG8Z3_002306"
     CDS             2280..2558
                     /locus_tag="ACG8Z3_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824593.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2655..3476
                     /locus_tag="ACG8Z3_002307"
     CDS             2655..3476
                     /locus_tag="ACG8Z3_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0824594.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(3761..4771)
                     /locus_tag="ACG8Z3_002308"
     CDS             complement(3761..4771)
                     /locus_tag="ACG8Z3_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0824595.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(4783..5736)
                     /locus_tag="ACG8Z3_002309"
     CDS             complement(4783..5736)
                     /locus_tag="ACG8Z3_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824596.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(5726..6796)
                     /locus_tag="ACG8Z3_002310"
     CDS             complement(5726..6796)
                     /locus_tag="ACG8Z3_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824597.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(6802..7845)
                     /locus_tag="ACG8Z3_002311"
     CDS             complement(6802..7845)
                     /locus_tag="ACG8Z3_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824598.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(7846..8787)
                     /gene="opp3b"
                     /locus_tag="ACG8Z3_002312"
     CDS             complement(7846..8787)
                     /gene="opp3b"
                     /locus_tag="ACG8Z3_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0824599.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            8871..9218
                     /locus_tag="ACG8Z3_002313"
     CDS             8871..9218
                     /locus_tag="ACG8Z3_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0824600.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9288..10949)
                     /locus_tag="ACG8Z3_002314"
     CDS             complement(9288..10949)
                     /locus_tag="ACG8Z3_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0824601.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            11128..11247
                     /locus_tag="ACG8Z3_002315"
     CDS             11128..11247
                     /locus_tag="ACG8Z3_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0824602.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
BASE COUNT         3173 a         2287 c         2046 g         3741 t
ORIGIN      
        1 ttctatgcga tttctgagcc atttggcatg gattttggtg cttcgattac ttgtaaagag
       61 ccatctggtg cttctgcaga aaggatcatt ccttgactga tttgtccacg catctttcgt
      121 ggtttcagat ttgctacaat gacgactttt ttcccgatca attcgcttgg atcaggataa
      181 aattcagcga ttcctgacaa gatttgacgg tcttggctgt ctccagcatc tagtcggaat
      241 tgcagcaact tatctgcccc ttctactttt tgacagttga tgacttctgc tacttttagt
      301 tctacttttt caaatacatc aaatttgatt tgtttttctt tcgttgaaac aagttctgtt
      361 tcttctggat cccatttgac tgtatcttcg tttgtttgag taccttcaga cattttttct
      421 tgaataaaag caacttcttc ttccatatct aaacgtggga agattggtgt accttttgca
      481 actacttttg ttcctgaagg aaattcgcca aagtgtaatc cttcaagatt cattgtttca
      541 gagtccaatc ctagttgatt gaagatcttc tctggcgttt ctgtcatgat tggctgaagt
      601 aatatcgctg cgatacgcaa agattcagct aaatggatca tgacactatt caattcagct
      661 ttcttttctt cgtcttttgc taatacccat ggttctgttt catcaatgta tttgtttgca
      721 cgagaaatca gcacccagat ttcagccaat gctgtattga attccatttt ttccattgca
      781 tcatggtaac gtccgacaac gttagcagcc gtcgtagaca gttcactatc aaatggagtg
      841 acaagtgagg catagtcagg aacgagtcca tcacaatatt tgttgatcat agcaatcgtt
      901 cgattcaata agttccctag atcatttgcc agatcataat tgacacggga aacaaaatct
      961 tctggcgtaa agacaccatc tgacccaaac ggaacagcac gaagcaaata ataacggagt
     1021 gcatctaaac cataacgttc cacaagcatt tcaggataaa cgacatttcc ttttgattta
     1081 gacatttttc cgtctttcat caatagccag ccatgcccaa atactttttt aggtagaggc
     1141 aaatcaaggg ccatcagcat gattggccaa tagatcgtat gaaagcgaac gatttcttta
     1201 ccgaccatct gaacatctgc aggccagtat ttttcgaata gactttcatc ttcggagcca
     1261 tatcctagtg cagtaatata attggataat gcatcgatcc atacatacac cacatgtttc
     1321 ggatcactct tgactggtac tccccaagta aaagttgttc gtgaaactgc aagatcctct
     1381 aatcctggtt tgataaagtt gttgatcatt tcgtttttac gagattctgg ctggatgaat
     1441 tcgggatgtt cttcatagta ttccaataga cggtctgcgt atttgctcat gcggaagaag
     1501 tatgattctt cttttaccaa ttctacttcg tgtccacttg gcgctttacc accgatcacg
     1561 ttaccttcgt catctttgta cacttctgca agttgtgttt ctgtaaagaa ttcttcgtca
     1621 gaaacagagt accagccttc atattcacct aagtaaatat ctccctgttc taataaacga
     1681 tcaaatattt tttgaacagc tgctttatgg taatcatctg tcgtgcgaat gaatttatca
     1741 taactgatat caagcgtttt ccataatttc ttgacatcag ccgccatttt atctacatat
     1801 tcttgcggtt ttacacctag ttcttctgct tttgtttcga ttttttgacc atgttcatcc
     1861 actccagtga gataaaacac atcaaagccc atcaaccgtt tataacgagc aattgcgtca
     1921 catgcaatcg tagtatacga gttgccgata tgaagtttgc cgcttggata atagattggc
     1981 gtagtgatat aaaaagtttc cttttctgcc acggggctct tcctccttta tcgcatgatg
     2041 cgaattcgtt tccttgctag tataccataa aaaaagcatg ctgttagctt tttttcaaaa
     2101 tgaaaaagac tggataaacg acttctatct acgttcaatc tatcctttaa aaggatgaat
     2161 aggttgaata aatgcgggtt tttgttttgt ttcatatcta ctaaaaaacg gctttttatt
     2221 tctcccctca cacgtggtat aataaagact atctatctta gtaagggact ggaacaatca
     2281 tgcagaagaa acatcttttt tttacactta gtattgcttt tctttcatta gcacatttaa
     2341 tattcagcta tttttatatt cgtatgtatg gttattttaa cctccatggg catttgaaca
     2401 gttttatgac agcagcttgg attttgcgtt ttatcattga cgtatatatc gttatttgtg
     2461 gcttctttgc cattcgtgaa gagcggtata aagtattgcc gttttatctg cttttctttt
     2521 tattcaatct catacttcca tttatttttc atatttaaat gataacctag atttttcaag
     2581 taaatccttg gttttttttc agagtctgct acaataaggt aaaaagtact tttactgtca
     2641 atggaggtgg tcagatgaac cgttttgctt ctaaagaaga agaaaaatta tattatgaac
     2701 ataaagcaag tgaagaagaa tttcttaact ggtatcaaaa acaggaacgt ccagaatatg
     2761 acaagccctc tttgactgtc gatatcgtac tgatgtgcta taacaaagaa gaagatcagc
     2821 tgaaagtttt gttgatccaa agaaaagggc atccttatcg aaattcatgg gcattgcctg
     2881 gtggctttgt ccaaaaggat gagtcaactg gagaaagcgt cttaagagaa acaaaggaag
     2941 aaactggtgt ggttatttca aaagaaaata ttgagcaact gcatacattc agtacgccta
     3001 atcgagatcc gcgaggctgg gtcgtcacag tcagctactt agcctttatt ggagaagaac
     3061 cgctaatcgc cggagatgat gccaaggaag tacgctggtt cacactagaa cgtcatagta
     3121 acaagatcca tttatctagc ggagaagtgg ctatctcgct tgatttagtc actggagagt
     3181 cttctggcaa agatactttg gcttttgacc atagtcaaat catcttgaaa gcattcaacc
     3241 gtgtagtcaa taaaatggaa cacgagccgc aagttctaca agttttaggt aaagatttca
     3301 cgattacgga agctcgaaaa gtatttgcta aattcttagg gatcgattat aaaacgatcg
     3361 atcattccaa ttttaagaaa gctatgctcc aatatttcga tgagatcggt gaacgtcctg
     3421 taggaatcgg ccgtccttca aaaatctatc agctcaagcc ggggcaccat gaataaggtt
     3481 gtgagcaaaa gattcagacc taatcattca agaaaaaacg caaaaacaac tctttgtgag
     3541 ttacgttttt tgtttcagaa ttttgatgta ttcgtaaact ttctgaataa aaaattaaca
     3601 atagcagaaa aagctcgtac gaactttttc tgctattgtt ttattggttt gtcataatta
     3661 gtgagctttt cttcaaaaga aaaattccta gaaacaaaag tcattaagac tttttattaa
     3721 caaggctaac cctaacgctc ctatcagttt cattcatttt ttatcccttg ataaccgaat
     3781 gtctgcccat tttttctact acttggctct ttcttacgga attgctttct tctgctccgt
     3841 cggtattgat caaaagaaat tttttattat accattggga cttataacgc aattttgtta
     3901 ttaatttatg ccagtcgtga atagaaaaat cagcatgtgc ctcttctttt ggaaaagatt
     3961 ctacaaataa gtagtcatat tgtttgacca atttcccaga taattgttca agataatctt
     4021 ctcgctggtt cttttgtttc agataaagat ccattacttt tctcttttgt ttctgataat
     4081 tgcttgcctg atctaacctg atctttctat ggtgagcgga gcggactttc acattcaatt
     4141 tccgctgtgc atgttgcagc ttttcttctg tctggtgaaa ttttggtaaa gttacttcta
     4201 gaggtcgaga tgtttggatc aatgtcttcg gatgataggc gatcccgatc catttagcta
     4261 aagattcttt ttttactggc gaaacatcac aaagtagtgc gacataaaat tcttcattat
     4321 atcgagcaga aatcgtagcg gagcgaatcg atccttcaac tggccgatgc tgatttatct
     4381 tgattagttc tttttgtttt ggtaacttta agtagccatt ttccagatag atcgtacggg
     4441 tttggttgtt ggtcgtataa gattgccaca tacttttttt cgttttaagc tttgggaagc
     4501 tggcacgtcc cttgaaataa ttgcgaaaag cccgatccaa attaagctgt gcatttgcta
     4561 aagcaagact gtctacttct tttaagaatg ggtattcttt ttttaatgta gcgggtgtga
     4621 gcgaaaaatt ggctactcct gtttgttgcc aacttttctg ccgctttttc agcaaatggt
     4681 tataggtaaa tcgagcacat ccaaaagtac gaatcaattg ctgtttttgt tcttctgtcg
     4741 gatacatacg gaatttaaaa gcttttagca tctatgtccc tcctagttag caaccagaag
     4801 agattccttt tgtcgtttta gtttttttgc gtaatatggg acttcctgct cagttgcata
     4861 gacaaaatgt tctggtgcga tttcacgaag ttgacgtact tgtccgtcat ctggttcagg
     4921 ttgatatttg attcttctgc gttttctctc atggtctgga tctggtagag gaatagcaga
     4981 taacaaactt tctgtataag gatgaacacc gtgattatag atttcatcac ttgttcccat
     5041 ctctaataac aagccgttat gcattacacc gattcggtca ctaatatgtt taaccatcga
     5101 caaatcatgt gcaataaata gataggtcaa ttgatgttct ttttggagat cttgcaacaa
     5161 attgaccact tgggcttgga tcgacacatc tagtgcagaa attggttcat cacaaatgat
     5221 aaaacgcggt tttactgcca atgctcgtgc aataccaata cgctggcgtt gcccacctga
     5281 aaattcatga ggataacgag taccgtggct tggattcaat ccaacagttc tcaatagatc
     5341 atcgactttt tttgcacgct cttcagatga tttagctagt ccattaacat caattccttc
     5401 ggcaatgata tctctgactt tcatacgagg attcaacgaa gcgtatggat cttgaaagat
     5461 catctgaaca tcatgccgga atttcgtcaa acctttttta tctttgatct tcgttatatc
     5521 ttgaccgtcg aataatatct caccatcagt aggttcattc aagcgaatga tcgtacggcc
     5581 agtagtggat tttccgcttc cagattctcc aactaaaccg aatgtttctc cttcataaat
     5641 gtgaaagctg atgtcattta cagcatgtac ttcattcttt tttcctacat tgaaatactg
     5701 tttcaatcct ttaacttcaa gtagtttatt tggcatcagc aattcctcct tgaccgtaag
     5761 tatgtttttg tttctcttta aagatagccc agcgacgttg gatttcttct ggcggtgtca
     5821 ctttgggtgc ctgcggagca agtagccaag ttgctgcctt gtgtgttttt gatagttcaa
     5881 aaaatggtgg ttcttgttct gcatcgattt tcatcgcgaa ttcatttctt ggatagaatg
     5941 cgtctccttt tggcgggttc aataaatccg gtggagaacc aggaatagca tacaatcgat
     6001 cgttcgcact atctaatgtc ggcattgaac caagcagtcc ccaagtatag ggatgttgcg
     6061 ggttgtagaa aatttcttcc gatgttccaa cttcaacgat tcttcctcca tacatgacag
     6121 cgacacgatc agcgacattc gctactacac caaggtcatg agtgataaag ataattgatg
     6181 tatcgatttt tgtctgaatt tctttcagca gctccaaaat ctgtgcttga atagtcacat
     6241 caagcgctgt tgtaggttca tcagcaatca ggatttccgg ataacatacc agtgcaatcg
     6301 cgatcacgat ccgttgacgc tgacctcctg agaactgatg cggataactg ttgatacgtt
     6361 tctcagcttc gggtattcca actaatttaa gcaattctaa tgcagctttt tgtccctctt
     6421 tttttgaaac tttattatgc tttctaaggg actcagcgac ttgcttccca atgggcatcg
     6481 tcggatctaa agaggtcatt ggatcttgga aaatcatcgc tatttcttta ccacggattt
     6541 tttgcatctc tctttctgat tttccaacga tatcgttacc tttaaagagg atttgaccag
     6601 catctatatt ggcgttgctt gacagcaagc gcatgatgct tctggtcgtc actgattttc
     6661 cacttccaga ttccccaacg atcgccaatg tttctccttt attcaaatga aaattgacac
     6721 cgcgaatcgc tcgtaccttt ccagcatacg tatcaaaaga gatttctaag tctttcactt
     6781 ctaaaatttt cgtcatcgtc ttcactcttt cattttagga tcaaaggcat cacgcaatcc
     6841 atcagcaagt aagttaaagc caatcatgat aaccgataaa gttactgccg gaatccataa
     6901 tagataaggc agatacagga atgtcttgta cccttcgctc aacatcgttc ctaatgaagc
     6961 tgacggaggc gtaagaccta aaccgataaa gcttaaaaag gcttcgaaga aaatagcaga
     7021 tggaatacta aacatcattt gaataataat gacactagag atattcggca aaatatgttt
     7081 gaatgcaatt ttcattcttg attcacctaa tgtcaatcca gctaaaacat attcttgatc
     7141 ttttaatttc attgtttgtg cacgaacgat acgtgccatt ggtatccagt ttgtgatggc
     7201 catagcagcc acgattgaaa aaattcctgg ttcaaaaacg accaacatca aaatcatgac
     7261 aacgaggttt ggaattcctg ataagatttc caaaaaacgt tgcatcacgt tatcgacacg
     7321 tccgcctaac aaaccagaaa ccaacccgta agctacacca atcgttacat ctagtaatgc
     7381 agcaataaat gcgatcagca acgagattct cattcccata aataagcggc ttaatacatc
     7441 acgtcccaaa ccgtctgttc ctaaaaagaa atttacgttt gaagggacgt ttgcttgagc
     7501 atatttatca acaagttcac caccaaccat tgcttttcca ttcaatccat cgatattgat
     7561 tcccggaata cgaggaggca aattgatata agccacgttt tgggcagttg gatcatgagg
     7621 agaaacaaag attgtgatca cagagatcaa aatgatgacc aatagtaaaa ccattgaaat
     7681 aacggcagct ttgtttttct ttaaacggcg ccatgaatct tggataaaat tcaaagaagg
     7741 tgcagtaatc tgttcacgtt cttcttcggt atttttttgt aatggaagaa attcatccgc
     7801 agggatatct gcaactgttt cataacgctt tggtatcatt tccatttatc ctcggctccc
     7861 ttctgaaaca cgaattcttg gatcaactag accgtacaat agatccacga tcaaaataac
     7921 aaaaatcagc ataaatgagt aaaggatcgt tacagccatg atcgtaggat aatcatttgt
     7981 tgtgattgac ttaacgaatt gttccccgat accaggaata gcaaagatat tttctaccac
     8041 cagtgatccg gtcatcaatg ctacagctaa cggtcctaat aaagtcatca atggaatcaa
     8101 actatttcga agaccatgtt taaaggcgat ttcccaacgg ctcaatcctt ttgcttttgc
     8161 taattccaca taatcactat gcagtacttc caccatttcc gtacggataa accgagcaga
     8221 gtcagcaagc ggcgacatgg ccaacgcaat tgttggtaag attgtgtaca caaagccatc
     8281 ccattttgcg attggcaaaa cttgtaattt gattgcaaag atatattgta ataaaacagc
     8341 aaatacaaag tttggaatgg aacgacctaa tatcgctacg agtgtagaag atgtatctgc
     8401 ccatgtattt tgcttcatgg cagagatcgt tcctaaaatc gtccctagga ctgttccaaa
     8461 gataattgcc tgtaacccta actgcaagga tggtccgatt cttccagcta acaaatgtgc
     8521 gactggctgg tttttaaatt ggaaggaaat tccaaaatct ccttgaagca aattagaaag
     8581 ataaatccca tattgtacga taactggttt atccaaaccg acttgtttat ttagcatctc
     8641 gattgtttca ggactcaaac gttcctgatt cgtatatggc gtgcccggca gcaattgcat
     8701 caagaaaaag gtgattgttg caattaacca aagcgtgatg atcatgaaaa agacacgttt
     8761 taatacatat ttgatataac tattcactaa tgtttccccc tagcttttga cttctgacag
     8821 atgtttcgct acattattag ataatgaacc aaaaataaag gagcaatcaa atgaaaaaaa
     8881 aatctatccc ctacgcagta gcttttctac tgattctggt tattttaata aaaaatctca
     8941 tcaaccattc ctttacactc attcaattat ctaatgatct attcttatgg tcattgccgt
     9001 ttctgattat cggcggtttt ttatgggtct tttcttcggg tttcttcgat cattttcagc
     9061 gatcggtcca tcttgcacga acaaggaacc gaaagaaaaa accagaattt tcctcgctct
     9121 cgtctgccag ctacggtatg tacagctttt ggctgatcat tgccggaata ctaattgctc
     9181 tttctgctat tttcatgctt ttttctttgt taggataagt gaatatgaga acgaggttgc
     9241 gacatttgcc gcagcctcgt tttttctcat gctcacggta ttaacgatta ttcttcaacg
     9301 tatgtccatt tgtaatccca tgaagcgcct gccgcatggt gaacgatacc tttgattttt
     9361 tcattcacta ggtgaccttc tgctttttgg tacaccggaa cgacacccat atcatcagag
     9421 atgatcttga ttgctttttg gaaatcatcc caacgggctt gttcatcgcc agcgtctgtt
     9481 gatgaagctg ctttaactgc ttcgtcatat tctgcattgc tccagcgacc acggttgtat
     9541 gaattacctg tgacaaacaa atcagtaaag ctgcttgggt ctgcatagtc agcagcccat
     9601 ccgcctagta ctgcgtcaaa atcacccgaa gttgagcggt caagacgtac agagaatgga
     9661 acaggtgttg gtttgactgt tacgccatcc aagttttctt ggattgagtt ttgaacatat
     9721 tcgattactt ttttcgatga atcatcatca gaagccatca aatcaaattc taaagaatca
     9781 acgcccaatt cttttttcgc tttttcccaa tattcttttg ctttatcttt gtcataagaa
     9841 actaattttc cagcttcttt tgcaaagtct tcgttcgttg ttggactttt taccatatct
     9901 gatgggatca atcctgtaga gacaactgat ccgtcaccta gtacttgttt tactaaagct
     9961 tcacgatcga tagaataaga aatagctttt cttaggttca cattgttgaa tggtgaattc
    10021 ttttcacgtt gattgaattc gatatagctt gtccgcgctt ctttaacaga agtataagct
    10081 ggatcgtttg cgttttgctg tgctaactca ccagacaaga tcacgtcatc tgcttggcca
    10141 tctttgaata agttcaatgc agtagaagat tcttttacta cactgacttt gatttcagaa
    10201 agttttactg tatctttgtc ccagtaatga tcatttttct tataagtcca ttctgtatct
    10261 gttcctggtc catcaaaatc ttctaatgtg aatggtccat tataaacagc tttatcactt
    10321 gcagatgcat aggcatcccc attttcttcc actacggatt gtttttgtgg gaaaaatgat
    10381 gggaaagcaa gcagatattg gaaatatggt gtttgtttcg tcaatttgat ttctaattca
    10441 tagtcaccaa ctgctttgat ccctagttct gatttatctt tctttccagc tgtaatgtct
    10501 tctgcatttt caactggcgc atacaggtaa gcatattcag aagctgtagc tgggtctact
    10561 gttcgttgcc agccataaac gtaatccgct gctgtaacag gatcaccatt tgaccatttt
    10621 gcgtcttctc gtaatttgat tttgtaagtt aatccgtctt cactgacttc tgccaactcg
    10681 ctagctcctg ccggctgtgg tttgctgtct tcatcaagac ggtaaattcc ttcataaacg
    10741 ttattcaatg ctgtaaaact gattgtatct gttgcaactg ataagtcagc tgtaggcatt
    10801 tcttgaggca cgactaagtt aaatacgcct cctccatctg ctgaaccgct ttttgctgaa
    10861 ctactgcttg tggctgaact gccgccataa catccagcaa gtacaaggcc gcataccgca
    10921 accaaaccaa atgtaaattt ctttttcatt ttacgtaccc ctttattgga attttcagaa
    10981 taattaatat tttctgaagt tgtttaagag tttaatgaat aattattaaa aaatcaacac
    11041 ttttttttgt ctttttcata aaataatgat tttttcgtga ggtttcaagg aatttttcag
    11101 aaaaaatagc tttgtacaag gttttccatg gtcttccaaa agccattaaa taattttcgt
    11161 tatattgtct attattgcca tacaaagata aaaaagaata aacaaaaaga aactgtgaca
    11221 aaagtcttgt cacagtccct tatctga
//
LOCUS       DAZKSU010000083        11121 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000083 DAZKSU010000000
VERSION     DAZKSU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.084"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..340
                     /gene="secG"
                     /locus_tag="ACG8Z3_002316"
     CDS             104..340
                     /gene="secG"
                     /locus_tag="ACG8Z3_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0824603.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            443..1198
                     /locus_tag="ACG8Z3_002317"
     CDS             443..1198
                     /locus_tag="ACG8Z3_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0824604.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            1201..3558
                     /gene="rnr"
                     /locus_tag="ACG8Z3_002318"
     CDS             1201..3558
                     /gene="rnr"
                     /locus_tag="ACG8Z3_002318"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0824605.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            3580..4044
                     /gene="smpB"
                     /locus_tag="ACG8Z3_002319"
     CDS             3580..4044
                     /gene="smpB"
                     /locus_tag="ACG8Z3_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0824606.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            4346..5065
                     /gene="atpB"
                     /locus_tag="ACG8Z3_002320"
     CDS             4346..5065
                     /gene="atpB"
                     /locus_tag="ACG8Z3_002320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0824607.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            5118..5333
                     /gene="atpE"
                     /locus_tag="ACG8Z3_002321"
     CDS             5118..5333
                     /gene="atpE"
                     /locus_tag="ACG8Z3_002321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0824608.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            5424..5948
                     /gene="atpF"
                     /locus_tag="ACG8Z3_002322"
     CDS             5424..5948
                     /gene="atpF"
                     /locus_tag="ACG8Z3_002322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0824609.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            5935..6477
                     /locus_tag="ACG8Z3_002323"
     CDS             5935..6477
                     /locus_tag="ACG8Z3_002323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0824610.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            6500..8056
                     /gene="atpA"
                     /locus_tag="ACG8Z3_002324"
     CDS             6500..8056
                     /gene="atpA"
                     /locus_tag="ACG8Z3_002324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0824611.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            8070..8972
                     /locus_tag="ACG8Z3_002325"
     CDS             8070..8972
                     /locus_tag="ACG8Z3_002325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0824612.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRAVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            8995..10401
                     /gene="atpD"
                     /locus_tag="ACG8Z3_002326"
     CDS             8995..10401
                     /gene="atpD"
                     /locus_tag="ACG8Z3_002326"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0824613.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            10415..10837
                     /locus_tag="ACG8Z3_002327"
     CDS             10415..10837
                     /locus_tag="ACG8Z3_002327"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0824614.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         3685 a         1982 c         2456 g         2998 t
ORIGIN      
        1 agttttgaac tttcagctgt tattccattt atatagagtt gtcacggact tttatctatg
       61 ataaagttaa agagacgata tcttttggga aggaggctgc gatatgtata atttaatttt
      121 gggaatcgtg atcgttttat cgattgttat ggtcattgcg attatgatgc agccaagcaa
      181 gcaaaatagt gcagcgagtg catttaccgg cggtgcggat caactattcg gcaaacaaaa
      241 agctcgcggc tttgaagctg tcatgcagcg atcaactgca gttatgggag ctatctggat
      301 gatcttatta tttgtcctag catatttatc ttcgaagtag ttgaataagc ctctcgcaaa
      361 acggcgggcg gcttttttta ttgcaaagag aacgcttaag aagaagttat gaaagaaact
      421 atggtaaaat gaggcgaaag aggtgagacg agtgagacaa ttaccaaaac ccctttatgc
      481 aaaacatgga aagcgagcgg ttttgctgct tcacgcttac tcaggaagtc caaacgatgt
      541 tcgaatgctc gcacgatttt tggaaaaagc agattataca gtttatgcgc cgctttttaa
      601 aggacacgga acaatggagc catatgatat cttgcaagaa aaagcagaaa gctggtgggc
      661 agacactaaa aaagccatcc attttttaca gtctgagcag ttttctgata ttgccgtatt
      721 aggattatca atgggcggca tctttgctgt acgagcacta gaagaagaat cagtgattgg
      781 cggcggtttt ttctgttcgc ctttgtctcc tgtaaaaaca aacgtgcctg aaaactttga
      841 aaaatacgta cgtcaagtgc tgaagacagc aggtaaatca gagaaagaaa tcaatgaaaa
      901 agcagttgct taccgctcgt tagctgaaca acaattaatg gatatacaag atcaggcagc
      961 aatcgtagaa agtaggttgt cagatatcca acagccgata tttttagctc aagctgggaa
     1021 agatgaaatg atcgacccta atggcgtatt tgaaactgca cgcaagttga gccgtcagcg
     1081 agtgacactt caatggtatc ctgaaagtgg tcatgtgatt acagttggta cagcaagacg
     1141 cgaattagaa aaagacgtct tggaattttt agaaaaatta ccttggaatg aggaataaat
     1201 atgacaaaag aaacgataaa agagaaaatt ctctttttca tggaaagcag tcgcaaacgc
     1261 agtttttcta tggaagaaat cgctgaaggt ttagggttcc aaaaaagcga tgattttaaa
     1321 ttactagtac aaacgattgc tcaaatggaa agagaacaga gtatagtttt caataaaaaa
     1381 ggaaaagtga aacttccttt aaaaccaatc ttgatcgaag gaacatttcg agcaaatgaa
     1441 cgaggatttg gatttgtaac gattgatccc gaagaagatg acatctatat cccaaaagaa
     1501 gcgactggtt ttgcaatgga tggagacacc gtagcaatcg atatcatgaa gcgagcaaat
     1561 acagatatgg atcgcggagc agaggggaaa gtagtcgaga ttcgagaccg ggcgactact
     1621 cagttagcag gggaattcgt tgcttattca gaagaagaaa tacaagaaac agatctttac
     1681 ggcgttgtta tcccgaaaga tagaaagctc aaccaattta aagtatatgc agcagcagag
     1741 ggagttcgtc cagttgacgg aagtattgtg ctgatcgaac tgacccatta ccctgaaaaa
     1801 gggtatgcga ctagtttaga agggataatc aagcaagtga ttggtcataa gaatgaccct
     1861 ggcatggata tcctctcaat tgtcgtggct aatggtatcc ctacgaaatt tcctgatgat
     1921 gtattgtctg aagcagatga agttccagat caaatcagcg aaaaggatat aactggacgt
     1981 agagatttaa gaaatcagct cattgttacg attgatggag aagatgcaaa agatctagat
     2041 gatgcagtta cggttaaaaa attagataac ggtaattatt ttcttggagt acacattgca
     2101 gatgtttctt actatgtgac acaaggcagt caattggaca gagaagcata tgaaagaggg
     2161 acaagtgtat atttgactga tcgagtgatt ccgatgatcc cgcaacgttt atcgaatggt
     2221 atctgttcac tgaatcccca tgttccaagg ctgaccatga gttgcgaaat ggagattgat
     2281 ccaaatggac aagtagtctc tcatgatatt ttcccaagtg tgatccaaac aacagaacgg
     2341 atgacctaca cagctgtcaa tcaaatctta gaagatcaag acgaagaaac gatgcaacgc
     2401 tatgctcagc tcgtacctat gttccaagaa atgggtgaat tgcatcgaat ccttgaaacg
     2461 atgcgaatca aacgtggcgc gatttctttt gaggatcgcg aagcgaaaat ccttgttgat
     2521 gctgaaggac acccaaatga catccagcta agaagcagag gaattggtga acgcttgatc
     2581 gaatctttca tgcttgcggc caatgaaacg gtagctgaac attataataa attaaatctg
     2641 ccgtttattt accgaatcca tgaacagcca aaagaagaaa aaatgcagcg cttctttgat
     2701 tttgcttcag ctttgggcat tctggtccga ggaacgaaag gaacgatcac accaaaagat
     2761 ttacagcatg tgatcgaagg agtagaagac aaaccagaag ctgcagttat caatactatg
     2821 ttgctaagaa gcatgcaaca agcacgctat tcagaagaca attttgggca ttatggctta
     2881 gcggcagatt actacacaca ctttacatct cctattcgac gctatcccga tctaatcgtc
     2941 catcgtctga tccgttctta cagtgaagat aaaagtgaaa gcaatcaaag ttcgtgggct
     3001 gcggaacttc ctgaaattgc agatcatagt tctaagatgg aacgtcgtgc agtagaaaca
     3061 gaacgtgaag tagattccat gaagaaagca gaattcatga tggacaaagt gggagaagaa
     3121 ttcgatggga tcatcagctc tgttgtgaaa ttcggcctgt ttatcgaact gccgaataca
     3181 gtagaaggat tgatccatat caatgaattg aaacaggact acttccattt tgttgaaaat
     3241 catttagctc ttgtaggtga aagaactggt ctgactttta aaatcggaca aaaagtgcgc
     3301 gtaaaagtag tcaaagctga tccagatgaa cgagcagtgg attttgaact cgtttccgca
     3361 gaagaggtca ctgcgttgga acgtccaaaa gcacgcaatg gaaaatctgg aaaacgagaa
     3421 ggaaaacaag caggcaaacg ctcagaaaaa aatggcaaga agaacaaacg atatagtgat
     3481 agtaagcaaa cgttcggtaa gaaaaaaggg aaaaagccat tttacaaaga tgtcaaaaag
     3541 aagaaaaaga aaaaataaaa tgagttggag gaaacagcta tgccaaaagg cgagggaaaa
     3601 ctaattgcac aaaacaagaa agctcgccat gattattcga tcatcgacac gatggaagca
     3661 gggatggtct tgcaaggaac ggagatcaag tcgatacgaa acagccggat caatctgaaa
     3721 gatggattta ttcgcgtccg caacggggaa gctttcttgc ataatgttca tatcagtcct
     3781 tatgaacaag gaaatatttt taatcatgat ccgttgcgca cgagaaagtt attattacac
     3841 aagaaacaaa tcgttcgtct tgaaaacgaa ttgaaaaaca ctggaatcac tgttgttcct
     3901 ttaaaagtct atattcgtaa cggctatgcc aaggtattga ttggtctggc aaaagggaaa
     3961 aaatcgtatg ataaacggga agatttgaaa cgaaaagata tcgatcgaca aattgatcga
     4021 acattaaaaa atttctctag ataatttcat tatccttttt cgcatattcg gtgcttttgc
     4081 ttttacttaa tgataaaatt tgttagcatt taatctgata aaacatgatt atcacaggtt
     4141 ttttcttaga tcaggagcac cgtgcatggg gaaaaattcg cttcgttttt gactgaaata
     4201 aaaaagtgtc taattttcat gaaaatgttt tgaattttct gtttcatggt ggtatgatac
     4261 cgtgtggtta gaaaccgatt ggaaaaaacc agatacgcac atgaattgat tttatgtgaa
     4321 tttcggggaa aagaggtgaa atggattgga tgaacgctcg ctaacgtttc acattggacc
     4381 aatttggttt gatggtacag tttgtataat ggtgttactg acctgtttga ttgttttctt
     4441 cttagcttac ttttttacaa gaaatcttaa gttgaaacca acaggcaaac aaaatgcgat
     4501 agaatgggtc attgatttta gtcgaggaat cgtaacagat aatctgccta gaaaagaatt
     4561 gagcaatttc catttgttgg catttacatt gtttttattt gtttttgttg caaacaacat
     4621 aggtttagta acaaaaatcg tcttacctaa cgaaacaaca ctttggaaaa gcccgacagc
     4681 agacccgttt gttactttaa ctttggcatt tataatgatc ttacttactc acttattcgg
     4741 ggtgaaaaag ttaggcttca aaggatattt cgtaaattcg ttcttgaaac cctatagttt
     4801 tatgtttccg atgaaactga ttgaagaatt tacaaattta ttaacacttg ctttacgtct
     4861 ttatgggaat atttatgcgg gggaagtttt gctaaccttg attgcaaaaa tgatgacaag
     4921 cttaggctgg ttctcattac cattagcaat tccattagag atggtctgga tcgcgttctc
     4981 cttgtttatt gggagcatcc aagcatttgt ctttgtcact ttgtcaatgg tttatatgag
     5041 ccataagatt gaagtagaag aataatactc ggttaaaaaa caaactacta tttatctatt
     5101 ttaggaggaa aacaattatg aattatatcg cagcagcaat cgcaatcatg ggagcagcaa
     5161 tcggagccgg ttatggtaac ggtcaagtta tctctaaaac aatcgaatca atggctcgcc
     5221 aacctgaaat gtctggtcaa ttccgtacaa caatgtttat cggggtagct ctagttgaag
     5281 ctgttcctat cttaggggta gttatcgcct tgattctagt ttttgccgtt taattgaatc
     5341 agtattaagg cagaaacaac atcgtttgtt actgcttttt tacatggaaa ctagaaatac
     5401 tccaaagaaa ggaaggctcg cctatgctga atcaattggc aattgcagaa gttggcaatg
     5461 cgatgttagg taatatcatc gttgtcagcg gctcattttt gatactactg gctctcttga
     5521 agcactttgc ttggggacca atcagcgata ttttgaaaaa acgtgaagac aagatcgcca
     5581 atgatttaga ttctgcagaa caatctcgca tcaactcagc gaaaatggaa caagaacgcg
     5641 aacaacaatt gttagcctct cgttctgatg cagctgatat catcaaaaat gcgaaagaaa
     5701 gtggagaatt aagccgccaa aatattttga aggatgctca agaagaagca gctcgtctaa
     5761 aaagcaaagc ccaagctgat atcactgtag aacgtgattc agcgctgaac tctgtaaaag
     5821 acgacgttgc agaactctct cttcaaatcg cggaaaaaat cttgaacaaa gaattatctc
     5881 cagaaatgca tgaatcatta atcaatcaat acattgaagg tctaggttct tcaaatgaaa
     5941 ctagataaat atacagtagg aagacgttac gggaaagctt tatttgaact ggcgatcgat
     6001 tcaaatcaag cagaagaaat ctatcaagat ctcgtaagtt tacgtcaaat ctatgaacaa
     6061 gtacctggat tagggaatat gttaagtgat gttcgtctag aacctcatga aaaacgagaa
     6121 atcatggaca agcttgtcag cggttatgat gggatcgtta aaaacttttt agaagttgtt
     6181 tatgagtata accgaatgga tgaccttttg ttcatgatcg aagaatatga acgtcgatat
     6241 gatgaacatc aagggttact attgggaagt gtcacaacag cagtcccact ttcagacgaa
     6301 caacgaatac aattagaaaa gaatgttgca aaaacaatgg actatcaatc agttgaatta
     6361 aaacaaatcg ttgattcttc tattattggt ggcgcgattg ttgaagccaa ccatcgagta
     6421 atcgacggaa gcattcgcac gcaattagaa aaaatgcgta atcaattaaa cagatagtac
     6481 aaagaggtag gtgaatcgaa tggccatcaa agcagaagaa attagtgcct tgattaaaga
     6541 acaaattgaa aattatcaaa acgtgctttc agtcgaagaa atcggtaccg taacttatgt
     6601 aggtgacggg atcgctcgtg cacatggttt agaaaacgcg atgagtggtg agttgcttga
     6661 attttcaaac ggctcatacg gaatggcgca aaacttagag tcaaatgatg taggtatcat
     6721 tatccttggc gatttcgagt ccattcgtga aggagataaa gtaaaacgaa caggtaagat
     6781 catggaagtt ccagttggag aggccttgat cggtcgggta gtcaatccgc taggtcaacc
     6841 aatcgacgga ctaggtgaaa tcgttactga caaagctcgt ccagtagaag cgatggcacc
     6901 aggtgttatg caacgtaaat ctgttaacga accaatgcaa acagggctaa aagcgatcga
     6961 cgcccttgtg ccaatcggac gcggacaacg tgaattagtc atcggtgacc gtaaaacagg
     7021 gaaaacttct attgcgatcg atacgatcat caaccaaaaa ggtcaagata tgatctgtat
     7081 ctatgtagca atcggacaaa aagattctac agttcgtaca caagttgaaa cattgaaaaa
     7141 atatggcgca atggattaca caatcgttgt gaatgccggt gcgtctcaac cagcaccatt
     7201 gctttatatc gcaccatatg ctggtactgc aatgggtgaa gaattcatgt acaacggtaa
     7261 acatgtattg atcatttttg atgatctttc aaaacaagct gttgcttatc gtgaactttc
     7321 cttgctactt cgccgtccac caggtcgtga agcttatcct ggggatgttt tctacttgca
     7381 ctcacgttta ttagaacgtg cagcaaaatt aagtgatgat ttaggtggcg gttcaatgac
     7441 tgccttacca tttgttgaaa cacaagctgg agatatctct gcttatatcc caacaaacgt
     7501 tatttcgatc actgatggac aaatcttctt agaaagcgac ttgttctacg caggtactcg
     7561 tccagcggtt gatgccggac tatctgtatc tcgtgtaggg ggttctgcgc aaatcaaagc
     7621 aatgaaaaaa gttgctggga ctttacgcct agacttagca agttaccgtg aattagaagc
     7681 atttacacaa ttcggttctg acttggatgc agcaacacaa gcaaaactaa atcgtggacg
     7741 ccgtacagta gaaatcttga aacaaaaatt gcatgcaccg ctagctgttg aaaaacaagt
     7801 agtgattttg tatgctttga cacatggatt cttagatagt atccctgtag acagtatttt
     7861 agacttcgaa catgaattat tcgaatatct ggataccaat cattcagata tctttgaaac
     7921 aatccgtaca acaaaagatc ttccagaaga agaagcgttg aacagtgcga ttcaggaata
     7981 taaagacatg ttcttagcta caaaaggaag taattcctct acagaagata aattgaaatc
     8041 cattcaaaat gcatagtgag gtgagatgaa tgggtgcttc attaaatgaa atcaagacac
     8101 gtatcgcttc aacgaaaaaa acgagccaaa tcacgcgggc aatgcagatg gtgtctgctt
     8161 ctaagttgac aaaatctgaa gcttcttcac agaagtttca ggtttatgca acaaaagtaa
     8221 gagaaatcgt tacgcatttg actgctactc agttaaatga tattgcctca gaaaatccta
     8281 gaggggatat caattacaac agcatgctgc tcagccgtgc tgtgaagaaa actggttata
     8341 tcgtcatcac tgcagatggc ggtttagtcg gtggatataa cagctcgata ttgaaacaaa
     8401 cgatgtctat tttagaagaa gaccaccaat cacccgatga ttacgtgatg atcgcaattg
     8461 gcggaactgg tgcagacttc tttaaagcac gcggtatcaa tctagcatat gagctgcgca
     8521 atctatctga tcagccaagc tttgatgaag tccgcaaaat cgtaaatatg gcaacaacta
     8581 tgttccaaaa cgaagtattc gatgaattat acgtatgtta taaccatcat atcaattcgc
     8641 taactagtca gtttcgtgta gaaaaaatgc tgccaatctc agacttagat ccagaagaag
     8701 caacgacttt cgaacaagaa tatatttttg aaccttcaaa agaagaaatc ttgtctcagt
     8761 tattgcctca gtatgctgaa agtttgatct acggtgcgat cgttgacgcc aaaacagcag
     8821 aacatgctgc tggtatgaca gcaatgaaaa cagcaactga taatgctgct aatatcattg
     8881 atgagctgac ggtttcttat aaccgtgcaa gacaaggggc tattacccaa gaaattaccg
     8941 aaattgttgc cggagcttcg gcactagaat agtattgaga ttggaggaaa aaagatgagt
     9001 tcaggcaaga ttgttcaagt aatcggtccc gttgtcgacg tggaattttc tttagaccaa
     9061 tccttaccag atatcaacaa cgcattagtt gtttataaaa atgacgaaaa taaatcaaaa
     9121 gttgttcttg aagcagcgtt agaattaggg gacggagtga tccggaccat tgcgatggaa
     9181 tcaacagatg gtttacaaag aggaatggaa gtcattgata caggcaaagc aatctctgtt
     9241 cctgtaggta aagaaacatt aggtcgcgta ttcaacgtac taggagatac gatcgattta
     9301 gaaacacctt tcccagaaga tgcggaaaga agcgaaattc ataaaaaagc accagccttt
     9361 gacgaattaa gtacaagtac agaaattttg gaaacaggga tcaaagttat cgatttgctt
     9421 gccccatatt taaaaggtgg gaaagtcgga ctattcggtg gtgccggtgt tggtaaaacc
     9481 gtactgatcc aagaactgat ccataatatc gcccaagaac atggtggtat ttctgtattt
     9541 accggtgtag gtgaacgtac tcgtgaaggt aatgacttgt attatgaaat gaaagattca
     9601 ggagttatcg aaaaaacagc catggtgttc ggacaaatga acgaaccacc aggtgcacgt
     9661 atgcgtgttg ctttgactgg tttgacgatt gcggaatatt tccgtgatgt agaaggtcaa
     9721 gatgtactgt tgtttatcga caacattttc cgtttcactc aagctggatc tgaagtatca
     9781 gccttgttag gacggatgcc ttctgcggtt ggttatcaac caacattggc aacagaaatg
     9841 ggtcaattac aagaacgtat cacatctacg aaaaaaggtt cgatcacatc gatccaagca
     9901 atctatgtgc cagccgatga ctacactgac ccggcgccgg caacagcatt cgcccatttg
     9961 gatgcgacaa ccaacttgga acgtaaatta acagaacaag ggatttatcc agcggtagat
    10021 ccattggctt cttcttcaag tgctttagca cctgaaatcg tgggggaaga acattataaa
    10081 gtagcaacag aagtccaaca cgtattgcaa cgatatcgtg aattacaaga tatcattgct
    10141 atcttaggta tggatgaatt atccgatcaa gaaaaagtgt tagtttctcg tgcccgcaga
    10201 gtccaattct tcttgtcaca aaactttaac gtggctgaac aatttactgg tcttccaggt
    10261 tcatatgtcc ctgtagaaga aacagtgaaa ggattccgcg aaatcttgga aggcaaatac
    10321 gatgatcttc ctgaagaagc tttccgaagt gttggccgaa tcgaagacgt cgtagaaaaa
    10381 gcgaaaacac taggctacta gaaaggggtg ccccatggat caatatttaa cagtaaatgt
    10441 ggtaactcct gccggtttgg tctatgatca ccatgcagct atcgtcgttg cacgtacgac
    10501 agctggtgag cttgggattt tgcctaaaca tgcaccaatc atcgttccat tgacgattga
    10561 tgaagttcgt gtaaaacgaa cagattcaga tacccacgtc gattggatcg ctgttaatgg
    10621 cggaatcatg gaagttcgtg ataatgttgt ttcaatcgtt gctgatagtg cggaacgtga
    10681 acgtgatatc gatgtatctc gtgcggaacg tgcaaaacaa cgcgcagaac gccaaatcga
    10741 agaagcaaaa gcaaaacaag atacgaacga attgaaacgg gcaactgttg cgttgcaccg
    10801 agcaatcaat cgtatcaaag tatctaagca ctcataaaat gagatgcgga tgtttatccg
    10861 tgtttaagaa cagtaaaaag aggttatgac agaagtggtc agcttcaaga cataagaagg
    10921 aatttacgaa aattgcttct caaatttttg tgaaatctca gcttattttt cgaagaagct
    10981 acttctggaa caccgtatat cagaggttgt aaaatcagcg ttttgtcctg agtattagag
    11041 aaacaaatgg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    11101 atgattgaac accgtttatt t
//
LOCUS       DAZKSU010000084        10803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000084 DAZKSU010000000
VERSION     DAZKSU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.085"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..312)
                     /locus_tag="ACG8Z3_002328"
     CDS             complement(28..312)
                     /locus_tag="ACG8Z3_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0824615.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(302..1036)
                     /locus_tag="ACG8Z3_002329"
     CDS             complement(302..1036)
                     /locus_tag="ACG8Z3_002329"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0824616.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            1165..1788
                     /locus_tag="ACG8Z3_002330"
     CDS             1165..1788
                     /locus_tag="ACG8Z3_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0824617.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            1980..3101
                     /locus_tag="ACG8Z3_002331"
     CDS             1980..3101
                     /locus_tag="ACG8Z3_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0824618.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            3108..6236
                     /locus_tag="ACG8Z3_002332"
     CDS             3108..6236
                     /locus_tag="ACG8Z3_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0824619.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            6255..9449
                     /locus_tag="ACG8Z3_002333"
     CDS             6255..9449
                     /locus_tag="ACG8Z3_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0824620.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(9595..10302)
                     /locus_tag="ACG8Z3_002334"
     CDS             complement(9595..10302)
                     /locus_tag="ACG8Z3_002334"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0824621.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         3786 a         1878 c         2218 g         2921 t
ORIGIN      
        1 tttttatatt taccgtgttt ttaacattca tatagattca ggaagcaaaa cggttgagtg
       61 tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt ttgaatgcag cttctgcttt
      121 tgttgcttcg cttcttgaag aaaatacttc tgcatggatc atcttaacag gtcttctttt
      181 ttgaagacga gtatatttag caccagtacc attgttatgt tcctctaatc gtctagaaag
      241 atcggttgta tatccaccat aaaatgtctg atctttacag aataatacgt aaaaataatg
      301 ttcatttgcc atagagcatc tcacgaatct ctgcagtgta ttctccatcc atattgtaag
      361 tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc ttctttgatt ccttcaacta
      421 acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa ttggatcctt ttaggtgcaa
      481 ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg atcaggacga tggaccatag
      541 taaagcgtcc atttgttttt agtagcttac ttgaaatatc gatcacttct tctaacgtcg
      601 tatgaatctc atgacgggcg attgccaggt gtttattagg gttcgtttta tttgttggca
      661 gccctttgaa ataaggtgga ttacagacga ctaagtcaca agaattatgt cgaacgacag
      721 ataagctgtc tttcagatcg atggtgtgga cagtcatttg ttcatttaaa tggttaagcg
      781 cgatactacg tcttgccatg tctgctagac gaggttgaag ttcgatagcg tcgatagccg
      841 cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt tcctgcacaa aggtcaacaa
      901 tcttcccttt tttaggtaca cgggaaaagt gagcaagcag cacagcatct aaagaaaaag
      961 aaaacacttt ggaactttgg ataatcttga tatcttctgc gtataattgg tcgattcgtt
     1021 cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa tagtcgtctg tattatacta
     1081 tttttattaa aaagaaccaa ggtggcttgt caaacctcta ttttttatgc atactatgac
     1141 cataagaagg gaaaggaaga gactatgttt tatcgattta tgcgcggggt agttcgagta
     1201 ctattagcct tagttaatgg aaatgcccgc tatgagaata aagaagtttt accgcaagat
     1261 gagaattaca tactagttgc tccccatcgt acttggtggg acccattata tctggcggta
     1321 gctgcagcac ccaaggaatt cagctttatg gcaaaagaag aactgttcaa aaatccagtt
     1381 ctgcgattta tattaactcg atccaatgct tttccagtta aaagagaaaa tccaggacca
     1441 agcgtcatta aaacacctgt aaagattttg aaatcgacaa atttgagttt gatcatgttt
     1501 cccagtggga caagacatgc tacagaactt aaaggcggaa tggcattgat ttccagaatg
     1561 tctaaagcga aaatcgtgcc agctgtatat caaggtccgt tgacacttgg ggatctgttc
     1621 aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg acttatcaga tatcaagaaa
     1681 acggataaag aaggtatggc ggagatcgaa cgacgaacac aagcagcttt tgacaaattg
     1741 gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa aaaaataaaa tgataagttt
     1801 atgcgtgggc ggtcttttga atctactgtc ctgttcagtt cagacttttt caatgaaaac
     1861 gagggagaga cgtcaagtct gccctctttt tgcgttccaa tatgtaaaat agaagacagg
     1921 tgtcatgtaa tggatcaaag tgttataata attagaagga cattttttga ggtgattgaa
     1981 tgcgtttttt acatacagcc gattggcata tcggaaaaaa actacaaggt tatgacttga
     2041 ttgaagacca gcgtcatgta ctatcttcta tactagatat cgcgaaagaa gaaaaagtag
     2101 aggcaatcgt gattgccgga gacttatatg ataggagtgt cccagctgtt gatgccgtag
     2161 aattatacaa tgaattgatg gtcgattgga atttaaagga aaagatcccg atttttgcta
     2221 tatcaggaaa tcacgatagc agtacgagac tttcagcggg tacgccatgg ttcagttatt
     2281 cgaactattt tttgcgaaca cagctagcag aagcatttga accagtcgag tttaaaaacg
     2341 tacaattttt ctttcttcct tactttgaac caattgcagc gagattatat tttgaagatg
     2401 acaatatccg aacgatcaag caagcggtag ccaaggtgat agataaaatg caggagctat
     2461 ttgattcaga aaaaaaacaa gtattgatta gccatttttt tgtaatggga agtgtaaaat
     2521 ctgattcgga aacgaaaatc gaagtaggcg ggttggattc gatccctggt gaactactta
     2581 caccgtttga ttatgtagca ctaggacatt tgcataatca agctgctttg agacaggata
     2641 atgctcgcta tagcggttcc ccattgaaat tctctttatc tgagatcaac caaaaaaaag
     2701 gcgtatggat cgttgacctg acaacaggcc tggagttgaa ttttagggag atcgaacccc
     2761 tgcatgatat tgaagaaata acagcgagtt tcaaagaatt gctggatcct gccttttatc
     2821 agtccgtcaa tcgggagaat tacatctata tctatctcac tgatagaacg atcatcccga
     2881 atatgatgaa ccagcttcgg caaatatatc ctcgtatttt ggggattgaa cgggcaaacg
     2941 gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga aatgaaagat cctcaaaaat
     3001 tagcagaagc attttttagt gaaatgacgg aagagcagct aactgcaaac caagaaaaat
     3061 ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg atcaagaatg aaaccgaaaa
     3121 aattacaact taaaaatttt ggtcctttta tcaatgagac cgtagatttt tcacgtttga
     3181 cagaagcacc gctttttttg atcagcggga agacaggagc tggcaaaacg acgatttttg
     3241 atgggatcac cttcgcactt tttggtgaaa cttccggaag acttcgctct ggaaaagaga
     3301 tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt gactttttct tttgaacatc
     3361 agcagatgag ctatgaaatc gaacgaaaac cagaacaagt tttagcaaaa cgaaaaggaa
     3421 acggaacaaa aaaacaggca gccaaagtaa cgttgaccat ttttgatggg aaaggaaaag
     3481 aaataaaaca aatcacaaaa cgtacagaag cagatcagtt gatcagagaa ctgatgaatc
     3541 tcgatgcgaa acagttttct caaatcgttt tactacccca aggagaattc cgtaattttt
     3601 tggtttcatc aagcagtgat aaagagactg ttttacgtca tttattcggt actcaatttt
     3661 tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca acagcaacaa aaattggatc
     3721 atttagagca agagctgcat cttctgcaaa aaaggtttgt tttagtcaaa gaagaggaac
     3781 cagcagcagg gtttgaagcc gttttagccc aatggacaaa ccatcaggca atactcgaaa
     3841 aacaaatcac ggaagaaaaa gaagctctaa cggaacttca ggaaaaacaa aagcaactag
     3901 aaaattccta ctatcgtttt gaaagcttga aaaaaagtta tgaagaaaaa acgatattaa
     3961 taaacaagca aaaggaatta gctgctcaaa aagacgaaat agaggaaaag aaacgatgga
     4021 ttgggtatta tgaatttacg caacgattag tcgaaccaat ccagcgccta caagaaataa
     4081 aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca aacaaagaaa ctagcgatga
     4141 ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa ccgcgtacgc cgacaaacaa
     4201 aaatcaaaga agctgctcaa aaactgcaaa acaaacagat gatggtgcca attgtcgaat
     4261 ccttacgtag taaaaaacaa gacgcagaac aattaacaat ttcattggat aaagaagaat
     4321 tgaatgccga gaagctgtat caaaatctcg ctatctatca ggaaagaact cgaattcttc
     4381 aagaaaattt actggatcag gaaaaactgc aagaagatcg tcttgctttt ggacagttaa
     4441 aatatctaga tgaaaagcag caagaaatag aaaaacaaaa agcaaaagac actgggtcgc
     4501 tcgcacagat aagagaagaa ctcaaaaaag caactgaaga aataacaaaa aatagtcagc
     4561 tattgaaaga acagacagaa caagcagaac agctgaaaag ccaatgggca aagcagcaaa
     4621 ttgctcgtct tcagcttttt ttgcgtccag gggagccatg tcttgtttgt ggatcaacgg
     4681 aacaccccat acaacaatta aaacacgaac agccaaataa ggaagatatc ttaaaagtag
     4741 aacaacaact ggcagaagct gagtctgctg tagaaagaac tcagcaaatc attgccaaaa
     4801 gtgaagcaca tgaaaataat cttcaacaac aattatcaga tttagaacaa aaaatcatgg
     4861 caacgaatat acaattggct gaacaagaag aacacttgca acaggctttg aaaaaggcat
     4921 ttcctgagat aacagaacaa aaaacaaaaa caactcttgt agtaatcgaa caatctttag
     4981 atagacgaaa aagcaagatg gccaatgcaa gaatagagct ggaagaaata agacaacaag
     5041 cagaaaagct cgaacaacaa ttagctgtct acaaacagca gcagacagaa aaaaataatc
     5101 agctccagca actgcaagga gaaatcaaaa gtttagaaca acaattgcaa gatacggata
     5161 gtaactgttt agaagaggag atacgacgac ttgaaaaaca gttagaagaa gactcgaatc
     5221 aactagctgt tgagcagaaa gcaggagtgc aattgttgca agatcttcat acgcttcgga
     5281 cacagcttga tcttcaacat gaacaaatag aaaaaataga tgaaaaaata gaaaaggaac
     5341 aagaaagaat agctaaaaag ttagaaagcc agtcgtattt ttcaacaatt gaagaaatct
     5401 gtgcatttgc acaacagcaa gcacaatata aagaaaacca acgtacagtt acttcttatc
     5461 atgaagagca attagtgaca gctgaccggc tgcgtcaatt agaaaatatc cagctaactg
     5521 agacattccc agatacagaa gagatcagcc aacagttaga agaaataaga cgtatgatcg
     5581 aaataaagca agaaaagata tacggtttac aagaacaaaa aattagtaat caaaaaatct
     5641 acgatgaatg tctttcgatt tatcacacaa gccagacgaa attagatgag cttacgcaat
     5701 tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa gaagactagt ttggaacggt
     5761 atgtgcttca ggtttatctt caagaagtgt tacaagtagc aaatgatcat ctccaacgtt
     5821 taacgaaaaa tcgttatcaa tttgagctag cggataccat tggcagttat cgagggaaaa
     5881 ccggattaga aatcaatatc tatgatgatc aggcaggtat gtctcgaagt gcgcataccc
     5941 tgtctggcgg tgagagtttt attgcagcac tttctttagc attagctctt gccgatgtga
     6001 tccagacaca agctggtggt gtagcaatcg atgcattgtt tattgatgaa ggatttgggt
     6061 cattagatga agaagctttg gaaatggcaa tggaagcttt agaaacgatc gaaagtgaag
     6121 gaagaatgat cggcatcatt agtcatgtaa gagaattgaa agaacgagta ttacaacaaa
     6181 taagaattga tacagaaggt agcggacaaa gcaaaatacg ctatcaactt ggataaaaag
     6241 ggagattggt aactatgaaa tggagtattc cagaaaaagt cattgcacgt ggacgtcaat
     6301 acttgcaaga aggccgagtg ttatcggttg tccctgacca agaagaaaac gtgtggcatg
     6361 cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga tggtacagca aaagagatag
     6421 attactgcca atgtccctat tgggaggaac ataaatattg taagcataca gttgctgtgg
     6481 aactttattt acggtcgaaa aatctatctc gtatcatgaa aaaagaccag ccaatgctag
     6541 aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa aggtttttct cgtctaaaaa
     6601 cacaaaacaa tcctcaagcg gtgcaacctt tgattattga ataccaagta gaaacgattg
     6661 agactaatca gtatcaccca gagctttcta ttttggcact ttacttgcgt atcggttcat
     6721 tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta tgatttttta caagcttaca
     6781 cagaaaaaga aacttacact gttaataaac agtaccagtt tcgtctagat aaacgggcgt
     6841 ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg aagtgctcag acgcatcaat
     6901 tattaggtac gaacggctta caagtcaaag gaaaactaga taaaagatat ctgcttctcc
     6961 cagtagaaca agcccgcttc ctgttagagc agatgaatca gactacacgt ttgcatctgc
     7021 agataaatga acaaaacgat caaggaatac aattttccga tcaaaacaaa cctttatcat
     7081 tcaaagtgga aaaatttgga gaagattatt tgctgcaagc attgaatgat tttgattttt
     7141 tctttatgca ttatcagtgg ggagttatcc aagggacatt ttattctctg acgaaatatc
     7201 aacaaacggt ttatcaaact tggaaacaat tgattcgccg attggagaaa ccagaagtag
     7261 tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat tatccctctg ctgtcagaga
     7321 tcggcgacgt agctattgcc cctgcagtag aagaggaagt agcagatatc cccgtcgaat
     7381 ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc tcgagtcgat ttcgtctatg
     7441 gcgaagtcat ctattcgact gacccgaagc atgaggtctc gacagaacag gcttttcagg
     7501 tgttacgtga cacagtagaa gagaaacgag tacttgatct attcagaatg tatcgctacc
     7561 agaaaaatga aactggttat gaacgtccat tgccaagcgg ggaagaacta tatgcctttt
     7621 tccgcacaga actatctgtc ttccgtcagt ttggtgaagt cagacttgga agaaaattgc
     7681 gagagttgta tttagatgcg cataaacatc aaccgaaaat cgaagtaacc gagaccggtt
     7741 catggctcga tattcgattc gatgtgacag ggatagagga gcaagaaatc gatcatgtcc
     7801 tgcaaagctt gctgagaaat gatgcttttt atacattaga aaatggtcag gttctttctt
     7861 ttgattcaga agaatttcaa caaacaagcc aagtgttgca acaattacgg gaatcgatac
     7921 gtacagaaga aggaacgatc catgtaccta aaaatcaagg attgatcatt caaaatcaat
     7981 tggaaaaaag caatgctact tttagtgaat cgttccaaac gatggtacaa gatctcattc
     8041 atccagaaag ataccaggca cagcttccca aaggattgaa tgcaacgatg agagattatc
     8101 aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta tcagtttggc ggtattttag
     8161 ccgatgaaat gggacttggg aaaacactac agacaattgc ctttttactt tcggaaaaag
     8221 aagaacggaa aagcttctct gctttgatcg tagcgcctgc gagcttaatt tacaactggc
     8281 aggcagaggt gagaaaattc gccccttctt taagtatcca agtaatcaat gggaataaaa
     8341 aagaacgtga agaattactg gctaaagaca cagatatccg agtgacttct tatgccagct
     8401 taagacagga tctagctaat tatcagtcgc aaaaaattga ttatttgatt cttgatgagg
     8461 cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc attgagagaa ttagctgtac
     8521 ctcagcggtt tgcactgagt gggacaccaa tcgagaataa tttagaagaa ttgtggtcat
     8581 tgtttgcaac gattatgcca ggatttttcc cgactaaaac aaaattcagg gaacttgccg
     8641 cagaagaaat tgcgcaaatg attcgaccgt ttattttacg gcgagataaa caaactgtgt
     8701 tgaaagactt gccggaaaaa acagagatga atctatattc tgcacttaca gaagaacaaa
     8761 aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga aatcacatca atggattctg
     8821 aagcattcaa gaaaaaccgg atcgggatac ttgctggtct gacaagactg cgacaaattt
     8881 gctgcgatcc tcgtctgttt attgaagatt accaaggcgg ttccggcaag ctcgaacaag
     8941 taaaagatct actggtagct gccaaagaga acaaacgaag agttttgctg ttttctcaat
     9001 ttacagggat gctgactatc ttgcaagaag agttagccga gctagggatc tcgacatttt
     9061 atttaagagg cagtacgaaa ccacaagatc ggttaagtat ggtagacgca tttaatgctg
     9121 gcgaaaaaga tgtttttctg atttccttaa aggctggggg aactggattg aatctcacgg
     9181 gagcagatac ggtcatcttg tatgaccttt ggtggaatcc tgccattgaa gaacaagctg
     9241 ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga agtgtggcgt atgatctctg
     9301 aaggaacgat tgaggaacgc atggattcac tccagcaaga aaaacgcgaa ctgttccaaa
     9361 aagtgatcca aggtaatgaa gaacagttga cgaaactgac tgaagaagac atccgcatga
     9421 ttttaagtgt aggagaaatc gaggagtaag agactcgaaa aagcgttcag taatgggtaa
     9481 gaaagccttt ctaaagaagc gattgtttat ttgatttaaa aaatagcaag gaggctggga
     9541 ttccatttta tcccagcttc cttgctatca tttttttatt tctattttta aaggttatac
     9601 aaagattgtc ttcattgtct ctaacagctg atcaaaagaa gaaatctcga tatcgcaaat
     9661 tggagtctgt tcaattttgc gttctcgatg gttgaaccaa agcgcttgcc agttagcact
     9721 ttttgcacct agaacatcat tgtcgtaatt atcgccaacg tataaggtct cttctggaag
     9781 catatgaaat tctttctcag ctagctgaaa aatctctttt tctggttttt ggaaacctgt
     9841 tgcttgagaa atgagcatat tgcgggaagg aatccaattg ttcaattgaa gttgcttcag
     9901 tttttttgtc tgatggtcag tcggcccatt tgtgataatt cctaatggga cattcttttc
     9961 cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag cagatattat caagttcttc
    10021 ctcgtaaatt ttttgaaata agaggccatc ttcttccgta atatgagggt agtctaaatc
    10081 ttttagtgat tggctgatcc gatgtgctcg catatattct agtgtccaat ctcctgccat
    10141 tacttttggg aagttttcat cactatgatg gcggaaccgg atatatagtg gatgcatatc
    10201 tgcatctgaa acaagcggaa cgactctttt gaccgcgttt cgaaatggct gctgctgatc
    10261 atacatcgta tcatcaacat caaacacaac tgatttaatc aaaattttac acgcctttcg
    10321 gttttcattg attttttcac aaatccttga ctattgtaac agaaaaaatt gtctaaaaaa
    10381 gctgataaat aaaggttttt cttactatat ttctatttcc ttgacagaat aaggttttta
    10441 tatttcagtt tttttcacta aaaatcgtga aacacgtcaa taagtttgat gaacaaatta
    10501 atcattatgg tagaagtcga ttgatttatt ttaatgcaat ctctgtggaa ttttcgaaag
    10561 atttattttt ttaatacagt ctcaatataa ctattcaaac ataaaataac gttatttgat
    10621 ttatttgttt atagtaaatt ttatttagta atcattgctt taaaatacta cgcttatata
    10681 acagagaaaa taagcgttat aataaagaac agtattattt aaaatgacag aaaacattac
    10741 tattaaagaa aaaaaaaaca taaaaaatct taagaaatcg gtttcattta gcttttgaga
    10801 taa
//
LOCUS       DAZKSU010000085        10576 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000085 DAZKSU010000000
VERSION     DAZKSU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.086"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..84
                     /locus_tag="ACG8Z3_002335"
     tRNA            12..84
                     /locus_tag="ACG8Z3_002335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACG8Z3_002336"
     tRNA            88..160
                     /locus_tag="ACG8Z3_002336"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACG8Z3_002337"
     tRNA            168..249
                     /locus_tag="ACG8Z3_002337"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACG8Z3_002338"
     tRNA            267..339
                     /locus_tag="ACG8Z3_002338"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACG8Z3_002339"
     tRNA            351..422
                     /locus_tag="ACG8Z3_002339"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACG8Z3_002340"
     tRNA            439..524
                     /locus_tag="ACG8Z3_002340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACG8Z3_002341"
     tRNA            535..608
                     /locus_tag="ACG8Z3_002341"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACG8Z3_002342"
     tRNA            618..691
                     /locus_tag="ACG8Z3_002342"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACG8Z3_002343"
     tRNA            711..783
                     /locus_tag="ACG8Z3_002343"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACG8Z3_002344"
     tRNA            811..884
                     /locus_tag="ACG8Z3_002344"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACG8Z3_002345"
     tRNA            898..971
                     /locus_tag="ACG8Z3_002345"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACG8Z3_002346"
     tRNA            995..1084
                     /locus_tag="ACG8Z3_002346"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACG8Z3_002347"
     tRNA            1097..1170
                     /locus_tag="ACG8Z3_002347"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACG8Z3_002348"
     tRNA            1189..1264
                     /locus_tag="ACG8Z3_002348"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACG8Z3_002349"
     tRNA            1266..1336
                     /locus_tag="ACG8Z3_002349"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACG8Z3_002350"
     tRNA            1350..1423
                     /locus_tag="ACG8Z3_002350"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACG8Z3_002351"
     tRNA            1448..1535
                     /locus_tag="ACG8Z3_002351"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACG8Z3_002352"
     tRNA            1557..1628
                     /locus_tag="ACG8Z3_002352"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACG8Z3_002353"
     tRNA            1651..1740
                     /locus_tag="ACG8Z3_002353"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACG8Z3_002354"
     tRNA            1753..1826
                     /locus_tag="ACG8Z3_002354"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACG8Z3_002355"
     tRNA            1845..1917
                     /locus_tag="ACG8Z3_002355"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACG8Z3_002356"
     tRNA            1921..1993
                     /locus_tag="ACG8Z3_002356"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACG8Z3_002357"
     tRNA            1998..2068
                     /locus_tag="ACG8Z3_002357"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACG8Z3_002358"
     tRNA            2081..2154
                     /locus_tag="ACG8Z3_002358"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACG8Z3_002359"
     tRNA            2166..2237
                     /locus_tag="ACG8Z3_002359"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACG8Z3_002360"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACG8Z3_002360"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0824622.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACG8Z3_002361"
     CDS             3826..5298
                     /locus_tag="ACG8Z3_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0824623.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACG8Z3_002362"
     CDS             5381..6169
                     /locus_tag="ACG8Z3_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0824624.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACG8Z3_002363"
     CDS             complement(6236..6904)
                     /locus_tag="ACG8Z3_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0824625.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACG8Z3_002364"
     CDS             7019..8731
                     /locus_tag="ACG8Z3_002364"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824626.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="ACG8Z3_002365"
     CDS             8734..10506
                     /locus_tag="ACG8Z3_002365"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824627.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3034 a         1926 c         2454 g         3162 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcaga
//
LOCUS       DAZKSU010000086        10510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000086 DAZKSU010000000
VERSION     DAZKSU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.087"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACG8Z3_002366"
     CDS             complement(220..972)
                     /locus_tag="ACG8Z3_002366"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0824628.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="ACG8Z3_002367"
     CDS             1085..2005
                     /locus_tag="ACG8Z3_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0824629.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACG8Z3_002368"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACG8Z3_002368"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0824630.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACG8Z3_002369"
     CDS             3311..4789
                     /locus_tag="ACG8Z3_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824631.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="ACG8Z3_002370"
     CDS             5066..7126
                     /locus_tag="ACG8Z3_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0824632.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="ACG8Z3_002371"
     CDS             7209..7778
                     /locus_tag="ACG8Z3_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0824633.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="ACG8Z3_002372"
     CDS             complement(7953..8759)
                     /locus_tag="ACG8Z3_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824634.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="ACG8Z3_002373"
     CDS             8897..10411
                     /locus_tag="ACG8Z3_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0824635.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3726 a         1752 c         2071 g         2961 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DAZKSU010000087        10383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000087 DAZKSU010000000
VERSION     DAZKSU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.088"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG8Z3_002374"
     CDS             6..305
                     /locus_tag="ACG8Z3_002374"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0824636.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG8Z3_002375"
     CDS             378..1739
                     /locus_tag="ACG8Z3_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0824637.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG8Z3_002376"
     CDS             1761..2021
                     /locus_tag="ACG8Z3_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824638.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG8Z3_002377"
     CDS             2035..2787
                     /locus_tag="ACG8Z3_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0824639.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG8Z3_002378"
     CDS             complement(2881..3486)
                     /locus_tag="ACG8Z3_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0824640.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG8Z3_002379"
     CDS             complement(3539..4513)
                     /locus_tag="ACG8Z3_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0824641.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG8Z3_002380"
     CDS             complement(4891..5610)
                     /locus_tag="ACG8Z3_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0824642.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8Z3_002381"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG8Z3_002381"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0824643.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG8Z3_002382"
     CDS             7161..8558
                     /locus_tag="ACG8Z3_002382"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0824644.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDFFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG8Z3_002383"
     CDS             8639..9079
                     /locus_tag="ACG8Z3_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0824645.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG8Z3_002384"
     CDS             9227..9553
                     /locus_tag="ACG8Z3_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0824646.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG8Z3_002385"
     CDS             9554..10303
                     /locus_tag="ACG8Z3_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0824647.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3291 a         1832 c         2151 g         3109 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaaatta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag atttttttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttt
//
LOCUS       DAZKSU010000088        10348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000088 DAZKSU010000000
VERSION     DAZKSU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.089"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..789)
                     /locus_tag="ACG8Z3_002386"
     CDS             complement(133..789)
                     /locus_tag="ACG8Z3_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0824648.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            982..1260
                     /locus_tag="ACG8Z3_002387"
     CDS             982..1260
                     /locus_tag="ACG8Z3_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824649.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1279..2487
                     /locus_tag="ACG8Z3_002388"
     CDS             1279..2487
                     /locus_tag="ACG8Z3_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0824650.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2635..3000)
                     /locus_tag="ACG8Z3_002389"
     CDS             complement(2635..3000)
                     /locus_tag="ACG8Z3_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0824651.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3147..3920)
                     /locus_tag="ACG8Z3_002390"
     CDS             complement(3147..3920)
                     /locus_tag="ACG8Z3_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0824652.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4094..4954)
                     /locus_tag="ACG8Z3_002391"
     CDS             complement(4094..4954)
                     /locus_tag="ACG8Z3_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0824653.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4954..6372)
                     /locus_tag="ACG8Z3_002392"
     CDS             complement(4954..6372)
                     /locus_tag="ACG8Z3_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0824654.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6429..6878)
                     /locus_tag="ACG8Z3_002393"
     CDS             complement(6429..6878)
                     /locus_tag="ACG8Z3_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0824655.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7323..7586)
                     /locus_tag="ACG8Z3_002394"
     CDS             complement(7323..7586)
                     /locus_tag="ACG8Z3_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0824656.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7685..8980)
                     /gene="rho"
                     /locus_tag="ACG8Z3_002395"
     CDS             complement(7685..8980)
                     /gene="rho"
                     /locus_tag="ACG8Z3_002395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0824657.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8977..10248)
                     /locus_tag="ACG8Z3_002396"
     CDS             complement(8977..10248)
                     /locus_tag="ACG8Z3_002396"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0824658.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
BASE COUNT         2902 a         2185 c         1793 g         3468 t
ORIGIN      
        1 ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
       61 atgaggaact atttttccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
      121 cctctttgtt catcaaaaga taatattgtc ttctaacaag tccatattca aaataacgat
      181 atgatggtct ttaacggtga taataccatc ttttttcagt tgttttaaaa ttctgtttac
      241 actgctagca gttgagatgc cacaaaattt ggcgatttct tcattcgtga tcgaaaaatc
      301 gatcagcatc ccttcttctg tccgcacacc gaataaatca tataattcgt aaagttgcgt
      361 acaaacagct cccagctttc cgtttaatag catttgctgc atttttttca tcgaataaag
      421 cagacgatag cggtaatact cttttacata gtgttgtagc tttgggtctt ggttgatatt
      481 ttgccaaaag cgcacacgat tgattcgata aaactctgca tattctgatt caactcgaat
      541 attatacggc gaatcgatga attgtgaata ttcgtctttt aaaagagaga cgatttcaag
      601 atctttgata tagcgaaggt taaattctct cccatcattt gaaataacac ttgcttttac
      661 cgttccctct ttcaagatat acgtatggga atcttccatc ccttcgaatg tcaaatattt
      721 atgatatcct ttgcgtataa tcggaaaatc atttttatcc aaatattcct ttaagactct
      781 actatccaat gatgctcccc catccaaact ttcatctaaa aagatcatac agtactttaa
      841 aactttttaa agtccaaagg tattttacta atgatattaa catttgttaa gtaccttttt
      901 ccatcactta tgcgattttt ttcctttttt ttattcgtta tactgcagaa gtaaaatttt
      961 ttaagaatag agggaaaaaa catgaattac tggatgaaca caatcattaa tcgattagaa
     1021 acagcatatc aaacaagatt tgatatgaaa gcttctttag tctttttaaa tgatgcgtat
     1081 caaaactcga tcgaattgat caaagcagtg gatgagaatc caacaaacga atgcgaagaa
     1141 tttttaaatc tgtttatgtc tacaagagat ttatttattc gccaacttgt tgatcgctat
     1201 ccatccaatt atcacgatgt agaagttcaa atacaaaaat taaaagcata ttcagcttaa
     1261 ggggaatgta taacatacat ggaaaagaaa ttatttaata aatattttat tggaatcaca
     1321 ttgatcaatt ttattgtcta cctgatttat tatctgttga tggtcatcat ggcagtcatt
     1381 gcgcaagaca ccttacacgc cagcttgagc caagcaggtt ttgcttctgg gatctatatc
     1441 atcggaacat tgctggcacg cttattcatg gggaaacagt tagaactatt tggtcggaag
     1501 tttaccttaa gaggcggcgc gatcttctat ctcctttcaa cattagcata tctttatacg
     1561 ccaactatcg gggcattgta tttagttcga ttcttgaatg gattcggcta cggtacagtt
     1621 tctacagcta caaatgctat cgttactgca tatatccctg catctaaaaa aggggaaggc
     1681 atcaattatt atggattaag tactagttta gctgccgctg ttgggccatt tttaggaatg
     1741 attttgctga atatgactga ttttcgtttc attatctggt tttcaattgt actggtcttc
     1801 tttgttacga ttgcctgcct tgtctttcct gtaaaaaata ttccattatc tgaagaacaa
     1861 aaaatatctc ttcgctcatg gaatatcaat agttttattg aaaagaaagc acttttcatt
     1921 acaggaattg cctttttgat gggtctttct tattcaagtg ttttatcatt tttatcttct
     1981 tatacaaaag taatccactt agttgctgct gggtcatttt tcttcgtcgt atatgctttg
     2041 gtcatcacat ttacacgtcc attgactgga aggattttcg atgtcaaagg agaacaatac
     2101 gtgatgtatc ctagctattt attcttaaca gcaggattat ttttactgag tgtaacgacg
     2161 aatagtgtga cattgcttat ctctggagca ttagtcggcc taggttatgg tacgtttatg
     2221 tcaaatggac aggctgtttg tttaaaaatc gtccgcgaac atcgaatcag tattgcttta
     2281 tctacctact ttattggttt agacctaggc ttaggcgttg gtccttattt gatggggatg
     2341 ctaaaatctc ttacttcatt tagaggattg tatgtgattg ccggagtcat tccattgata
     2401 tgtacagcac tctatcttgt taatggccga aaacatagtc atgaagcaga gggcgaattg
     2461 attgaatcat tgaaaaatga aatttgatct tgatgaatag ataaaaaaaa ccgttcagat
     2521 ctgaaaataa tctgagcggt tttttgagta aaaatagttt atgattattc ttaattaaac
     2581 atctatttat ttttaaagct ttcatttatc gataagaata gtttaactgt tgatttattc
     2641 cattttccgt tcatctggta cgatgccttg acttagcaac cacttgtgga agaaccattc
     2701 tgccacaccg atcaatagag caggaatcaa tactttcatc caaatatcga ctgtatcatt
     2761 cataaaatat agatagagcc acaagataac tgcagctgaa ccagcatctg cgatcgtcgc
     2821 tgcgatgttc cctactttac gtaagatcaa cagatcccct agcacataga tcatgactac
     2881 aataattacc ccaacaatcg ttgaatccat aaagctaaca ccgtataaac ctgtcagcac
     2941 gacccacaga atcgcccaga tcatgattgc cttcactacc aacgccttca catgtttcat
     3001 tttctgcact cctttctcct ggataaaaaa gtaagattat ctaacaagat aaccttttca
     3061 ttattatacc atcggctgag agaagcgagc aaagataagg tcctatctaa aaatgaggaa
     3121 gccacttaga acattcacgt aataatttat ccgtacagac attcgatatg gtgttccttt
     3181 actttcgcat actgttcttt tgtcagttct ttacttgtca ccagataatc gatttcatga
     3241 aaagcaatgg cttgatgcat gaatttatgt gcaatttttt gctgtgtaac taaaacggca
     3301 agccggtctg attgttttct aaaagctttt ttgaccattg cctctccttc atgagtttct
     3361 gttactcctt ggctgaaatc tagcccgcga caactcatta tcgctaaatc cgcataatat
     3421 gacaatgcat cgttataggc ttttgaaccg ttgattgttg ctcgtttcgg aacaattgcc
     3481 cctcccaata cagatacttt tgccgttgta tattcactca acatagcagc tgtgacgatg
     3541 ccattcgtaa tgatctgcaa accatcaaat cttgctaggt ttctgactaa gtgtaaagag
     3601 gttgtactgg aatccaagaa taagaccata tcatctcgaa tcaaagaaga agctgtatct
     3661 gcaatcttac gtttttctgt ttcttcttct aatttggtga tcttcatcag gtaaggcacg
     3721 tcaatttttc gattgtcaat aagcatggcg ccaccgcgaa ctctacggat caatccactt
     3781 ttttccagaa aaacaaggtc acgtttaatc gtggcttcgc tataatttac taactcgatc
     3841 aaatcggtga ctcttgcaaa ttggttgttt tttagataat tcacaatctc ctgctgtcta
     3901 ttctccactt cattcaacat aaaatcaccc catttacttt atatagtaac aggaaaaaag
     3961 aggttgtgac agaagtgatt ggcttcgaga aataagaaga aatttttcga aaaaactact
     4021 tctgaacatc gtgtgtggtt aagagggtat gacaagacta ttgtcatacc ctcttgacca
     4081 cacattttta tttttagtat aaactagcct tgcctactgt gttcaaaatt tccattttat
     4141 gtttgaccac ttcttgtacc tttttattag ctgctggata aattgcattc ggctcgtaca
     4201 tcgatggatt ttcactcaat atttctctca aagcattgaa aaagacgctt ttaagatctg
     4261 agctcaaatt cactttggca actccgtatt ttactgcttc gctgacttct gcgtccggat
     4321 tacctgaacc gccatgtaaa acaaatggaa gatcgatttg ttggttcaat tctttcagta
     4381 agtcgatatt taattttgga gttctgtttt tcggatacaa gccatgcgct gttccaattg
     4441 ctacagcaag tgtatcgatt cctgtttctt tcacaaaaat cttcgcttga tcaggatctg
     4501 tataaatgat tgtttcagca cctccttcag cagaaccatt attcccaatc gttccaagct
     4561 cagcctccac tgagacattt accgcatgag ctaacgcaac cacttttttc gtaatctgta
     4621 cattgtcgtc gaatgatttc aaagacgcat cgatcatcac ggaagaatag ccgtttcgaa
     4681 tcgcccgtaa gacatcttct atcgtaccgc catgatccaa gtggataacg atcggcagtt
     4741 tacttgtata agcataggct cttactgccg caacaaactc atcacttaaa taggcgattt
     4801 catcaggatg aatttctaag attactggtg cttctaatct ctctgcttct tcgatgattg
     4861 ctttcatcat atcatagctg caaatattga aggctggtac tgcaaattga ttctctttag
     4921 ccactttcaa taactctttc attgtatata acattacgct tccacctcat ttgttagatt
     4981 ttcatttttc gtccacttgt cttgtttttt ctctttcact gtttttttca atagtccaag
     5041 cataaaggct gtagtgactg tacctgatgc aattgccaag acatacatag gtaaattgtt
     5101 cataacattt ggaattagca tcacccaaat ccctccatga ggtgccattg aagtaactcc
     5161 tactgccatg gaaatccctg cagcgactgc tgaacctgtc atcaaactag gtatcacgcg
     5221 gatcgggtca gataccgcaa acggtatagc accttctgta atatacgaag cgcctaaaat
     5281 ccagcatgat tgacctgaag tccgttcttc tttggtaaat ttatgtttga ataataaagt
     5341 ggctaaagca atacctaggg gaggtgtcat tcctgcagcc atacaagcag caatcggtgc
     5401 atttactcca gctgacatca aaccgatcga gaaagtcgaa gtcgatttat tgatcggccc
     5461 acccatatca gctgccatca tgatcccgat aagtaatccg aacaatagcc cggatgtatt
     5521 tcccaagcta ttcagccaat ttgtcaaagc atccaacacc catgtgatcg gttgttcaat
     5581 cacaaatacc atcaaaagcc caataattaa gatcgataaa aaggggacga caatcagctg
     5641 ataaatagca gaaaattctt tttttacgct aattttattt gccagaaaat cacagatata
     5701 ccctgctagc acgccaccaa tcattccgcc aagaaaacct gaaccacctg tagaggccag
     5761 taatccggca acaattcctg gggcaaaacc tgcttttcca gcaatcgatg tagcaatccc
     5821 cgcgcctaac gcaggaacca tcatggcaaa cgctgtatct cctccgatcc ttgaaaatgc
     5881 agctgctaac acattatagt ctgctgagtt gggatcagcc gctgtaatcc caaaggcaaa
     5941 actcagtgcg atgatgattc cgcctgcgat cacaaatgga agcatgtaat tcacaccatt
     6001 catgaaatga ttatagattg tcggcttttt ctcatttgca gctggttctt tttcattaga
     6061 cacaacgaat acgccaatat ctctcaatgc ttcgtctatt actacttgcg gttctttgat
     6121 tgctcggctt gtagagacac gctttacttt tttcccggca aatcggtcca attcgatttc
     6181 tttatctact gcaagaatga ttgcatctgc ctctttgatt tcttgttcag ataactgatt
     6241 ttctgttttt acaccttgtg tttcgaattt tgcttgatgg tttagatttc ttgctgttac
     6301 ttctaatttt tctgccgaca tataagtatg agcaattcca gtcgggcaag cagttacacc
     6361 aacgattttc atgattattt tcctcctttt tcttcactat cgatactcgg acaaagactc
     6421 tactattttc attcttgact aatctgtctg atgatatctg ctggcgtagt ggcttctttt
     6481 attccctttc taaaatctgc attcatcaat cgtctggaga gtgtgctgag aatctctaga
     6541 tgcatatctg ctgatttttc aggaactacg atggcaaaaa tatattcaat tggcatttca
     6601 tctatcccgc tatagtccgt taatttggct gtatgaaaaa aacacagtgc tggttttgtc
     6661 acatatttgc tttttgcatg gggaatccca aatccatctt ctattcctgt ggagatctcc
     6721 tcctctcgtt tgaatagtgc cttaacgact ttttgaacat ttcttacctt atttgttttt
     6781 ttcaacacat cccctattcg ctcaaataat gatccttttg aatctatcga tggatcgaaa
     6841 acaatcagtt cttctgcgat caattgatca aacctcataa aaaacctctc ccccacaaaa
     6901 aatgttaacg ttttcttgga actgaggtca gtatagcatc gctagctgta ccgttcaata
     6961 ttatgaatga acagaggaaa aagacccatc atccgcgtgt ataagtcatt aaaatgaacg
     7021 atctagctca caaacaatcg caagtctata acaaaacaaa aagccttcct tgaaaggaaa
     7081 gcttagcttc taattttagg taaatggttt tttacttcga aataacccct cttcatttcg
     7141 ttaatataag gtgtttttat caagtggata attaaaacta tatactagtt tttcattgtt
     7201 ttgtgcatta tgatcctacg actttttcag catcaaacga agttttaaat tggcaaaaaa
     7261 cataaaaaaa cagactatcc ctatcagata gtctgctaaa cgttgagtta tcagcgtttt
     7321 tattattccg cagcgtttgc gtctttgaga ccgtattttt tgttgaaacg gtccacacgt
     7381 ccgtctgctt gtgtgaattt ttgacgtcca gtgtagaatg gatgtgagtc agaagttact
     7441 tcgacacgga tgactgggta tgtattaccg tcttcccatt caactgtttc ttgtgaagtt
     7501 tttgttgaac ctgacaagaa tttgaaacca gttgttgaat ccatgaatac tactgggtgg
     7561 taatctggat ggatgttttc tttcatgatt ttttcgctcc tttgccctgc tccatttgct
     7621 gaaccagagt tgatttgtcg atttacaaca ttaacatctt aacatgtttc agtcaaggtt
     7681 gcaattatct tttcagattt ctggtcggat tttttttacc aaaggagaca tcttgaaatt
     7741 ctttgaaaaa ttgctgattg ttttttgttt tacgcaaaaa gcggatcagt tgttccgtat
     7801 attctaatga atctccagtc atattttttc gaagtttcca aatttcttct aattcttcac
     7861 ttggcatcaa taattcttct tttcgggtac ctgatttttt gatatcgatg gctgggaaga
     7921 tccgacgttc agataattca cgtgaaagtt gtaattctag atttccagtt cctttgaatt
     7981 cttcatagat cacgtcatcc atacggcttc ctgtatcgac taaagcggtc gctaaaatcg
     8041 tcaaactgcc accttcttcg atgtttcttg ctgccccgaa aaatttctta ggtttataca
     8101 atgcggctgg gtcaatcccg ccactcaatg tacgtccgct tggtggaacg actaagttat
     8161 aggcccgagc aagtctagta atactatcca tcaagataac gacatctcgt ttatcttcga
     8221 ctaaacgcat tgcgcgttcc aatactaatt cagaaactcg agtatggttt tgcggctgca
     8281 gatcaaatgt agaagatacc acgtcgcctt tcacgcttcg ttctagatcc gttacttctt
     8341 ccggtcgttc atcaatcagt agaacgatta attctacttc cggatagttt tcggtgatac
     8401 catttgcgat ttcttttaat acagaagttt tccctgcttt tggcggtgcg acgatcaatc
     8461 cacgctgacc aaaaccgatt ggagaaaaga tatcgatcat tcgggtagat agtctttgcg
     8521 gtgttgtttc cagttgaagc tgtctttgcg gataaagagg tgttaaggct ggaaaatgcg
     8581 gacgttcttt tgcttcttcc ggatcttttc cattaacgct ttccacgtgc atcaaaccat
     8641 aataacgttc cgattcttta ggtggacggg cttttcctgc cactttgtcc ccatttctca
     8701 aactgaatcg tctgatttgt gaggcagaaa tatagatatc ttctgcactt ggtccataat
     8761 tgattggccg taaaaaaccg taaccatctt gaccaacgat atccaatatt ccttccatat
     8821 aataaaatcc ttgtttctct gcttgtgcac gaatcacagc tagagataat tcctttttat
     8881 tcatcttact gtaataagga attttaaatt ctttggcata actataaact tcttttaacg
     8941 ttttgttctc tagttctgcc attgtcaaat aatcgctcat tcagctccca cttcctctat
     9001 catttcaatt gatgccccta aggctgttag tttctcgata atatgatcat aaccgcgtaa
     9061 gatataatct acattgtaaa tcgtcgttgt cccttctgcc aatagtccag caatcactaa
     9121 acaagcacct gcccgaagat cagaagccac gacttctgtg ccgtgaagtt tatttggacc
     9181 attcacgatg atcatatttc cttccacggt aatatcagcg cccattcgtg ctagttccgg
     9241 cacatggttc acacgtttcg catagatcgt atcgatgatt tctgaagtgc ctgtagtcat
     9301 caatagtaac gcagtaagtg gctgctgcag atctgtggca aatcctggat aaggataggt
     9361 catgatgttt gcgggtttta attctttcga ggggtaaact tcgatttcat cttcgctgat
     9421 tttcatatgg acaccaattt cctgcaattt tgctataaag ctttccaaat gttcaaagat
     9481 cacatttttc actttgatac catttcctac tgcagctgct aatgctagat aggttcctgc
     9541 ttcgatgcgg tcaggaatca tgaaatgccg acagccatgt aatgtttcga ctccttcgat
     9601 acggatcaca tctgtacccg ctcctcgaac ttttgccccc atattattca gtaaagttgc
     9661 cacatctatg atttccggtt ctcgtgcagc gttttcaatt actgtctgtc cttttgcttt
     9721 tacagcagct aacatcacat tgatcgttgc tccgactgaa accacgtcca tgaaaatccg
     9781 attgccacgc aatcctttat tttctgtccg taaatacatg gcaccatgtt catttgtcac
     9841 ttcagcacct agagcttcaa atcctttgac gtgtaggtca atcggtctag ggcctaaata
     9901 acagccacct ggcaggccta ctaccgcttc gccaaattta ccaagcagtg ttcccataaa
     9961 ataataagaa gcacgcaaac tattgatttt tccgtgcggc atcggaatcg atacgacttc
    10021 tcgaggatcg atttctagta tgttgttgga aaaatggaca gttgcgccca tgatctctaa
    10081 aatttcaatt aatgaatgaa catcttgtat gtccggcaca ccttccaaga tcactgggga
    10141 atcagctaag attgcagctg gaatcaatgc gactgcacta ttttttgcac cgctgatcgt
    10201 tacttcacca gctaaaggtc ggtttccttt gattacgatt tttttcatct gtttccacct
    10261 tgtttattaa tcttttttca tggatatgct tgtattttat catagataaa aaaaagaaac
    10321 gagcatggtc tgccgtttta agtaaaaa
//
LOCUS       DAZKSU010000089        10057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000089 DAZKSU010000000
VERSION     DAZKSU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.090"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(523..747)
                     /locus_tag="ACG8Z3_002397"
     CDS             complement(523..747)
                     /locus_tag="ACG8Z3_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824659.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            938..3715
                     /locus_tag="ACG8Z3_002398"
     CDS             938..3715
                     /locus_tag="ACG8Z3_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0824660.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3796..4215
                     /locus_tag="ACG8Z3_002399"
     CDS             3796..4215
                     /locus_tag="ACG8Z3_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0824661.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4227..4697
                     /locus_tag="ACG8Z3_002400"
     CDS             4227..4697
                     /locus_tag="ACG8Z3_002400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0824662.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4714..5493
                     /locus_tag="ACG8Z3_002401"
     CDS             4714..5493
                     /locus_tag="ACG8Z3_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0824663.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5480..6301
                     /locus_tag="ACG8Z3_002402"
     CDS             5480..6301
                     /locus_tag="ACG8Z3_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0824664.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6318..7367
                     /locus_tag="ACG8Z3_002403"
     CDS             6318..7367
                     /locus_tag="ACG8Z3_002403"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0824665.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7380..8384
                     /locus_tag="ACG8Z3_002404"
     CDS             7380..8384
                     /locus_tag="ACG8Z3_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0824666.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9017..10036
                     /locus_tag="ACG8Z3_002405"
     CDS             9017..10036
                     /locus_tag="ACG8Z3_002405"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0824667.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3373 a         1655 c         2096 g         2933 t
ORIGIN      
        1 taaatgttta tagatgaaac tctatttact tcttttttaa tttgttcttg caatcttttt
       61 tgctacgtaa tttttgtaaa aaagtgggtt gagagaaatc taattgattt tcaaagaagg
      121 agtatgaaaa tgagaaaaat atttattcgt ttattgtggg tactatctta gaggaataaa
      181 taatagtgga gaaaactatg gtttggcgca gattctatca ggtttgatgg aacgaagaca
      241 acctcccgac atcaagtcaa aagctacttg agattttgat ctgaaaataa aaaaagaaaa
      301 caaggtagga acatcaatgt ttctaccttg ttttcgtaat caatgggaaa aaactaagag
      361 tggtgtacgt aatcaataaa aaggatggtg acaacagtac ctctttactc tctgaaagca
      421 tcataactcg tttttcctca ttatgccaat aaattgttgt tataccacat ataaggattt
      481 gtaatagcta aaaacagaca ggaaagttga aatctgtgaa tcttattttg tatggaagga
      541 tttatagaac gcaaaaatca agataaagat gaataaaaat cctgtttcgg ctatatattg
      601 cgtatctacg ccaataaaac gagtttgttc tgtcatagcc atataaagca aaggcaaaaa
      661 tacaactaca gcgagataag atccgatgat ttctcgaaaa gttttccagg aagtatgttt
      721 cctgttttta tatttatccg atttcattga atagtcatag cctccttatt ctttcatatg
      781 ttgaaattcc agtataaaaa ctggaaggaa ctgtacgttt tttagaataa aaattataag
      841 ggataagtag gctgctgtct ataataagat atatttttca attctttatt aaagatttta
      901 ttgacaaaat gaatccgttt tctatgatgg aggaggggtg aataagttgt tgaagaagga
      961 aattttatgt tacttagaga accagacagc ctttttcgat ttagaacata tgaacgaggt
     1021 ctttacagca caaaggttag ctgatcattt tggggtaaaa agaaacacag tcagtcatta
     1081 tttgaaccag ttaaatgatg agcaactact agtcaaaatc aattctcgac cagtgattta
     1141 tttccacaaa gaagcttttg aaaaacaatt ttttaagtta cgcacgaatt tttatttttc
     1201 tatcgatgat ctcaaagagg aacagccttt tttcgcgcaa ccaaaagact tattctcttt
     1261 tatgatcgga tacgacgcca gtttgaaaga aagtatcgaa caaatcaaaa cagctcttta
     1321 ctatccagat ggcggattac cacttttgat tacaggcgaa agtggcacag gtaaaagtta
     1381 tttagttaat ttggtctatc agtattgtct gcttcatgat ctgctagaag attctgcacc
     1441 attcatcact tttaattgtg cacaatatgc agataatcct gaacttttga ctagcaattt
     1501 gtttggacat gttaagggag cgtacactgg tgcagaagaa aataaaaaag gggcttttga
     1561 agcagcagat ggtgggatct tgtttttaga cgaggtacat cgcttaacac cagaaggtca
     1621 ggaaaaactg ttcacctatc ttgatcaagg tgtgatttat cgtatgggag aacctaattt
     1681 gtcacgccgg atcaaaacac gtctcttttt tgctacgact gaagagatta ctagtcattt
     1741 cttaacaact ttcattcgaa gaatacctat ccgaatcgaa ctacctgcac ttaatcagcg
     1801 tagtcgtaat gaacggctag aactgattta ctcttttttt ctcgaagaac aaagaaaaat
     1861 ggggagccct ttgtcagtca gtggaccagc tttgtcactt ttaacaagtc agaaattatc
     1921 cggaaacata ggagagctga aaaatatcgt caaagtttca gcagcaaaag catatgctga
     1981 acagcgtgaa cagcctgaaa tccatgtcac gatccatcat ttgcaaaaag aactacttgc
     2041 gcagcctatt actaaaaata cagcacagca aggaatctat attacgcaag accagacctt
     2101 agagcaatta atggagaagc aacagcccga acagcagcga atcgttaaaa gttttgagca
     2161 aatcctaatt gatttcaaga aaaataactg tctgctatat tcttgcgaag gaaaattaaa
     2221 gcaagaagtg attcaattat ttgatttttt gctgtttgag accagtagac aagaaaaaca
     2281 cgagctgctg gtttattgga cacaatctat ccgagagacc tttcgacaaa tggaaactgc
     2341 ctatcaaatc aaattcgatg gaaacagtgt ctatgccttg agttactatc tctatcagcg
     2401 aagcactatc aaatggttac cggaagaaca tgatattatc caactaatca aagaattaga
     2461 acaacaagtt tctcagtctt atccatctag ttatcactat gtccaacgaa ttctggaatt
     2521 aagtaagatc aaattagata ttgaagacac aagtatggat cggattttac tgacgatcta
     2581 tctaaaaaaa gcgaaatggt caaaagaaaa acgactgcca aaagcgatga ttgctgctca
     2641 cggttatgca actgctagca gtatggcaaa tgtagtaaat cgtttattac aagaggatct
     2701 atttgaatca tttgatatgc cactagatgt cacaccacag cagattgctt cagaaatcct
     2761 tgattattgc gaaaacagcg atgtttcaaa tggattgatc atattagtag acatgggatc
     2821 tctgaaagaa atccatcagt tttttaaaaa acagttatcc gtaccgcttt tgattttgaa
     2881 taatgtgacg acacctttag caatcacagt aggggaatgc ctgcaaaaaa atgtatcatt
     2941 agaagagata gcagaagaga ctgttcgaca gatccagcca gaatggcgac tgctctatcc
     3001 agaggaaaac aaacccaaag cactcattac tacttgcttc actggaatcg gtacggcggt
     3061 ccacttaagt gaactgctag aaaaaagctt acctacggct tgtcagttga aaatcatccc
     3121 atatgaatat cagcaattaa aagataagaa aaacagtgac ccattatttt ccatatacga
     3181 agtcgtagga atgattggca ccacagatcc tgatatcacc aatatcgact atctttcatt
     3241 agaagacctc atctcggggg aaaagatgtc tgtactagct gaatggttgg acactactat
     3301 gaatgcttca gagaaggaaa tattcaatca gcgcatcatt cgtaattttt cattggaaaa
     3361 agtactggat tccgtgacta ttctagatac agagaaggtg atgggagaaa tcgatcattt
     3421 tatgagagaa ttagaggtgc agttgaatcg aagcatcagc aatcccaaaa aattagcgtt
     3481 gtatgtccat gtgagctgtc tcattgaacg attgatccga caaatgccaa tcgaaaccta
     3541 tcaaggatta gagaaatgga agcagtgtca aaaagaaggg ttatctgcta ttaaatctgc
     3601 gtttagtgtc attgaagagc attatagtgt catgatcccc aattctgaga ttgcctatat
     3661 ctatgatatt ttgagtgaag atacagagtt tctctcgata gaagaagaat tttgaacata
     3721 agcaagttgt tgccactaat tttggcatgc aacttgcttt tttagtatgt gtaagtgaaa
     3781 agaggagatg aaaaagtgga tagagaaatt atcttagctt cccatggaaa atttgcttca
     3841 gggatattag attcattaga gctgatctat gggaaaaatc attctattac tgtattcgat
     3901 tgctatactg atgagaattt tgatttagcg gaaacagtaa ggcagctttt tatgaggtac
     3961 aaagataaag aaatcattgt tctgacggat ctttttggcg gaagtgtcaa caacgaattc
     4021 ttacaatata tcaatcggga tcatgtctat ctcgttgccg gattgaattt gccactattg
     4081 attgagctag tatctcggtt ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg
     4141 ctaactgatt caaaaaaaat gatccagttt tgtaacgagc ggctcaatca gaaaatagaa
     4201 gaagacgaat tttaggagga agaaaaatga ttgtattgac aagagtcgac catcggttat
     4261 tacacggaca agtagctttt tcatggacac aaacaatagg cgcagactgt attttgattg
     4321 ctaatgatga tgtacctacg aatgagatac gaaagacaac gatcaaatta gccaagccac
     4381 aaggagtcaa actagtgatc aaatcaattg atgactcgat tgccgcgttg aaaagcgggg
     4441 tgacagataa atataagtta ttcatcgtag tggaatcgat cgaggatgca tataagctgg
     4501 cagaagcgta tccagaaatc aaaaccatca acttaggagg gatcaaggca aaacctggta
     4561 ctcgcagcat cggaaaagcg gtaaatattt taccagaaga ggaggaacgc ttgaaacgat
     4621 taattgacaa acaaatagaa gtagaaatcc gtcaagtacc agcagataaa aaagtggatg
     4681 taagagaagt tttgtaagaa aggaagaacc tcaatgattg tacaagcagt cttattagga
     4741 attgttgcat ttgtcgcaca atcagaatat gcactaggga catcgttgct ttctcgtccg
     4801 attgtgacgg ggctgtttac aggaatcgtg ttaggtgatg taaaaactgg gatcattatg
     4861 ggcgcaactt tagagttagc atttatcgga tcgttctcag taggtgcttc gattccacct
     4921 gatgttgtga ccggtgggat tttagggacg gcttttgcta ttacagctgg agccggtaca
     4981 gaaaccgcgc ttttactagg tttgccgatt gctacgttga cattgatatt gaaaaatatt
     5041 tacttagggc ttttgattcc aattatgaac cataaagcag acaattatgc agaagaagga
     5101 aattataaag gagtcgagca tatgcacttg cttgccggta tcggtctttc cttgatgctt
     5161 ggtgttgtgg taatggtttc ttatatggtg ggaagcaata cgatcggaaa tctattaaat
     5221 atgattcctg acttcgttca aaaggggttg tcagtagcta cagggttgat tccagctctt
     5281 ggttttgcga tgcttgctcg tttgctgatc aacaaacaag ttgcaccata ttttttctta
     5341 ggttttgcta tagcttctta cttagagatt cctgtgaccg gagtagctat ttttggtgcg
     5401 attttagcag ttgtggtagt caatattatg aatgtccgtc aactaccact tcaaacaact
     5461 gcaggggagg tagaagaaga tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa
     5521 agagctaaat cacgtatttt ggcgttcttt tcaaatggag ttttcttgga attatgaacg
     5581 tcagatgaat atggcttatg tttatgcaat gattccgatt ttgaaaaagt tatatcagtc
     5641 gaaagaagaa atggcagcag ctttgaaacg gcatctagag ttttttaaca cgaccccaca
     5701 tatcgttaca ctgattttag gaatcacggc tgcaatggag gaagaaaata aggatgatcc
     5761 tgaatttgat gtgactgcga tcgatagtat caaaacatct ttgatgggac ctcttgcagg
     5821 gttaggagat tctttctttt ggggaacttt gcgattgatt gccacaggtg tgggtacctc
     5881 tttagccatg caaggaaata ttttaggccc tatcttgttc attttgattt tcaatattcc
     5941 gcacattctg tttcgctatc tagcaacagg ttggggatat cgcttaggaa cgggtttttt
     6001 gaaaaagatc caggcaaacg gtatgatgga aagtttgaca ttaggcgcgt ctattattgg
     6061 actgatggtc gttggagcaa tgactgccac gatgatcacc atcaatattc ctttaaaaat
     6121 cggttcagga gaaaatgctg tgactgttca aagtattttt gatgatatcg ttccaaatat
     6181 cttatgtctt ggagcatttg gcatagtgtt ttatctattg aagaaagaag tgaaaccatt
     6241 aacgatcttg atcggactag ctttctttgg aatttttggt tcgtggatag ggatttttta
     6301 aaaaaggagc agagaaaatg tttacgatgc aagattatat ctatgaagaa aaggaagtac
     6361 taagtacgat cttgaaaaag aatgattttt cgacaagaga acatatgaaa aaaacagtga
     6421 atttactgat cttagcaacg ggttcatctt ataacgcttg tttagcagcg aagccagcat
     6481 tggaatctta tggtgatctt actgtcgata ttcaagaacc tttttatttt ttcaattatg
     6541 gaaaactttc tccttcaatc gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt
     6601 ctactgttga agcagtaaaa atgatacaaa aacaagggtt acctgttgtt gctatcacaa
     6661 atgacgtgca aagtccactt gctttagaag ctgcacaaat tattgattta ggtgccgggg
     6721 tagaatcagt aggcttcgtt acaaaaggct attctgcgac tgttttacag ttactactct
     6781 tagggatagg aattggaata agtaaaaata aaatcagcaa aaaaatagaa caagactaca
     6841 tgcaacagtt gcgcaaaata atcaaccatt taccagcgat tattcaaaaa acggaagaat
     6901 tttttgatgg atatcaaagt ttatttcgtt tagcacagcg tttcgtcacc attggatatg
     6961 gtccaaactg gggaacagct aaagaagcag agacgaaatt gactgaaaca atccgcgttc
     7021 cgtcacaagg atttgagtta gaagcttata tgcacggccc atacctggag gcagatgctt
     7081 cgcatttgct attctttatt gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc
     7141 aaagatatat gagtcggtac gttggagaga ctctcaccat taccacaaaa aaagcaagaa
     7201 acgagaaaac acttggatta gcaatagaat gtgatgaata tctgtctgta ttagcgcttg
     7261 tcgttccgtt tcaactattc gcttataaaa ccgcagtagc taaaggtatt gacttgaaca
     7321 aaaagatttt tgaagacttt gatacagtct tgaaaagtaa attataagag gtggaaatca
     7381 tgctgaaatt caatgaagaa gaacaaatca aagcaataaa aggcgcacta gctttacgtc
     7441 cgcaagtaga agaaatcatc gataaacttt atgcggaaaa atttgacgct gtctattatc
     7501 taggaatcgg tggaacgtat gcttcctcga tgcaagctgt cacttatatg aatgggaaaa
     7561 gcaacttgcc tgtctttgta cagcatgctg cagaatatta cactactgga aacaaaagat
     7621 taacaaaaga ttctatcgtt attttgtcct ctgttaccgg tacaactcaa gaagtagtga
     7681 aagcagtaga acaaatcaag aaagtaggag cgacactgat tggatttatc gataaagcga
     7741 acagtaagtt gtctcagcta tgcgattttg tggtcactta tccagctcct ggaacagaac
     7801 aaatcaaatt ttttatggca gcagatcgat tgatgtattt gaatggagaa ttcgaagctt
     7861 attcagaata ctatgaacag ctcgaacgtt atttaccaac agggttagtg gaagcagaga
     7921 aaaaagcaga tgcatttggc ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt
     7981 attttattgg agcagggaat caatggggag ctgtttattc atatgccatg tgttactggg
     8041 aagaacaaag ctggcttcca tcgaaatcta tccatgctgc cgagtttctt cacgggacgt
     8101 tagagatcgt agaagaaaca accccagtca cattattttt aggcgaagat gaacagagag
     8161 tgttatctga acgtgtggct aaacttttgc caaaaatttg cagcaattat acattgatag
     8221 acacaaaaga ctatccagta gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt
     8281 tcttattgat gcatgtagtg acacaacgca tcgatgctca tgtggagcag ttgaactgcc
     8341 atccattaga gatacgccgc tactatcgtc agtttgatta ttaggataga ctggataaaa
     8401 agaatataaa aaataaagag agcagcgata actgctccct ttattttttt aaaatattgg
     8461 catcaattac gttataaaac acattttttg cattatctac agttcaactt ataatctgtt
     8521 taatgggaca tgtgggtgcc aaccattttt tgttatgcaa tagtactatt tagttgttgc
     8581 atggatccgt agtaaaagtc caagtacaac gagttgcgcc agtttacata gcgttttgta
     8641 tccagaatgt tgactaaaac aaaagttcct atgtcaaccg tcaacagaga caatctgtcc
     8701 gttgcaggac ctgacagatt tagaaatgtt caatctgtct cttatgtatt ttaaatcaat
     8761 actcactagg agaagtttcc taacctccta atagtaatgc tggtagtaac attgtcaaaa
     8821 ctgcttcctc ttaggaacaa gcaaagcata acgagtgtaa aaataaaatg tagactattg
     8881 taacatatca ttgattattt caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa
     8941 caatggaaat tagtcccaac tttaagaaat gtatactata atggaaatta tcaattattt
     9001 atttggagga atatccatga aacaaaaaat cgcagttctt ggtcctggat cttggggcac
     9061 tgctttagca caaacattgg cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc
     9121 tgaacagatt gatgaaatca ataagtatca tacaaatcag cattttttac ctgatttgac
     9181 gatcccagaa tcgattatcg gatataaaga attggcagaa gcagtggata acgcagatgc
     9241 tattttattt gttgttccaa caaaagcgat tcgttcagtc gcacaggaac ttattggtaa
     9301 aatgaatact aagccaatca ttatacatgc aagtaaagga ctagaacaag atacgcataa
     9361 acgaatctca gaagttttgg ctgaagaaat tccagaagaa aaaagacagg caattgttgt
     9421 attatctggt cctagccatg ctgaggaagt ggcagtacat gatatcacaa caattacagc
     9481 tgcaagtatt gatcaaaaag cagcagccta tgtacaaaaa ctgttcatga atgattattt
     9541 caggatttat acaaataatg atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat
     9601 cattgcaatt ggtgccggcg ctatccatgg attaggattc ggagatgatg caaaagcagc
     9661 aatcatgact cgaggattgg cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc
     9721 tctgacattt atcggtttga gtggtgtggg ggacttgatc gttacttgta caagtgttca
     9781 ttctcgcaat tggcgtgccg gtaacttgtt aggacaggga cataagctgg aagaagttct
     9841 agaaaacatg ggaatggtcg ttgagggtgt ggcaacgaca aaagcagcag tagagctagc
     9901 tcaacagcta ggtgttgaga tgccaattac tcaaacaatc tataatgttt tgtataatgg
     9961 agaagatatc aaaaaagcag caaaagaaat catgttacgt gacggaaaaa tggagaatga
    10021 attttctctt cgttaaatag atgatcaata gttaatc
//
LOCUS       DAZKSU010000090        10043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000090 DAZKSU010000000
VERSION     DAZKSU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.091"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(285..1268)
                     /locus_tag="ACG8Z3_002406"
     CDS             complement(285..1268)
                     /locus_tag="ACG8Z3_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0824668.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1273..1920)
                     /locus_tag="ACG8Z3_002407"
     CDS             complement(1273..1920)
                     /locus_tag="ACG8Z3_002407"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0824669.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(2005..2637)
                     /locus_tag="ACG8Z3_002408"
     CDS             complement(2005..2637)
                     /locus_tag="ACG8Z3_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0824670.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2653..3204)
                     /gene="coaC"
                     /locus_tag="ACG8Z3_002409"
     CDS             complement(2653..3204)
                     /gene="coaC"
                     /locus_tag="ACG8Z3_002409"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0824671.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3201..3938)
                     /locus_tag="ACG8Z3_002410"
     CDS             complement(3201..3938)
                     /locus_tag="ACG8Z3_002410"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0824672.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3935..4924)
                     /locus_tag="ACG8Z3_002411"
     CDS             complement(3935..4924)
                     /locus_tag="ACG8Z3_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0824673.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5182..5724
                     /locus_tag="ACG8Z3_002412"
     CDS             5182..5724
                     /locus_tag="ACG8Z3_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0824674.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5741..6826
                     /locus_tag="ACG8Z3_002413"
     CDS             5741..6826
                     /locus_tag="ACG8Z3_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824675.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6838..7644
                     /locus_tag="ACG8Z3_002414"
     CDS             6838..7644
                     /locus_tag="ACG8Z3_002414"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824676.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7645..8472
                     /locus_tag="ACG8Z3_002415"
     CDS             7645..8472
                     /locus_tag="ACG8Z3_002415"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824677.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8469..9542
                     /locus_tag="ACG8Z3_002416"
     CDS             8469..9542
                     /locus_tag="ACG8Z3_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0824678.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3060 a         1839 c         2039 g         3105 t
ORIGIN      
        1 aagaacagcc aagtaaaaac agcttcttat gtttttaact tggttggact tattaccgca
       61 gatgcaagct tttgaacacc gtttattcgg aaaaagagcg tgggacaaaa gtaaagaaca
      121 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagaaatt ccttcggaaa
      181 taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg
      241 agttaaacgc ttctgtccca gcctcttttt tgatcagcat gattttattc agttatcttc
      301 aaaatgattt ttccgatgtt tttgtttgct tccatatatt catgcgcttg tttgactgcg
      361 ctgaattgga atgtacggtc aatgattggc cgaatcttgc cattttctag aaaaggccca
      421 gctttttgca taaaagtttg tgcgagctct gctttatact gatcacttct aggtgtcaat
      481 aatgtaccaa gcagttggac acgtttccca atcaatacac caaggtctat ctcaggcacg
      541 atggtcccac ctagcatacc aatcaagacc caacgtccat caacggctat agaagctaaa
      601 ttcttttccc agtaagaagc gccgatgaaa tccaaaataa catcgactcc ccgaccttca
      661 gtgatttttc gtacctcttc ttgaaagtct tgttttttat agttgatcaa ttcatctgca
      721 ccaagttctt tacaaaaggc gagtttttct tctgatcctg cagtaacgat gattttcgcg
      781 tgagaaagct gtctagctaa ttggatcgct gctgttccca caccacttgc tcctgcatgg
      841 atcaagacag tttcgccttc ttgtaatttt ccaagccagt aaagtgtttg ataagcagtc
      901 aaaaaaactt ctgagattcc agcagcagaa acatagtcta gcgtatcagg gagaaggatt
      961 gcacgatcgg caggcatcac agcatattcg gcataaccac ctagattgac taatccgtat
     1021 acttttgtcc caggagtaaa agtggagata tcacttttat tctcaactac ctctccagct
     1081 acttcgactc ccaaaatagg gtagggaggt ttcaaagaga ctgattctct tttcataata
     1141 tctgttcgat tgacagctgc tgtatggact ttgatcagta attcgccttg ttttggttta
     1201 ggaacaggca cttcaatttg ttctaataca tctacagaac caggatgctt gattgaaatg
     1261 gcacgcatgg atctattcct cctttgccca ttcttggaat gctgtattca actctttgct
     1321 accttcagaa agtactagag tcgtacgaga aatatccgtt tcttccacta gagcggcaag
     1381 tacagcagca actgtagagc gagtgatcgt ttttgatgcc ttctcattag gcttagttaa
     1441 ttgaatggaa gtaacttctt catcgttagt caatgtgact ggttgaagaa tgacaaaatc
     1501 taagttagtt cgtttcagcc attcatctgc ataatgttta gtaatataat aatctgtcat
     1561 ggactcaggc cattttgctg gttcatctgc ataaacagca ctgaccatca gatagcgaga
     1621 gacatgcgca ttttcagctg cgatcactgt tttgattgcg ccatccaagt ctacttgcag
     1681 aagattcttt ccttgagagc cggcagcaaa aatcaataca tcgatgcctt tgaatgcttc
     1741 ggtcattttt tccacggacc aagttaaatc aaaagagaca tacgaaacat tctctttttt
     1801 tactaatgtt tgggaagcaa catctctcac ccctgcaaaa atttcatggg aacttggagc
     1861 taaatctttt atcaggtgtc ggccaatttg tccgtttgca cctacaacaa aaattttcat
     1921 ttggatcatc ctttcgacgt caaatctttc cattcaatag taacaccaaa agagaaaaaa
     1981 atcagagatc ggctacgatg actatcagtt agtaagagaa gggtgcttga agtatcttaa
     2041 caagacattg catactgctg cagtcgcaag acctgcgacg attccttcag gtataccact
     2101 tgtaaaaata accgctagaa tagctgaata aacagcattt aaatttgttc cgatgacttt
     2161 ggcataatct tgctggaata aaaagtagat catattcatc actaaaatcg tattggtcaa
     2221 tgaaccggaa agtccagcta agaataaaga aacggaatct gctttttgtt tcattagttt
     2281 gcgcagacct ttataaacga aataaggaac caccccaatc aagatgcgag gaataaacgc
     2341 gatcagcaag gctttccagc ttccatgttc tgttccgatg actgggataa acggagaaaa
     2401 gacaaaagac aaaggtgttt ggataactgt gctacggatc atgctgatca gtccaaatgt
     2461 accgccaaga aaagcaccta gtcttggtcc taaaacgatc gaagcaatga ttacaggaat
     2521 atgcatggtt gttgcattga ttggaccgat agggataaaa cctaaaggcg taacagctaa
     2581 taaaatcaaa atagctaaaa acatcgcagt taatgtaaag ttttttgtat ttttcataag
     2641 tactcctttt ttctaagcat ttctttcatt aagagtcagc atgacctgtt cgatgatcgt
     2701 ctgaatatca gctaaggcac cttttccaaa atctccacaa gctaaaagag attctcttgg
     2761 ttcaatttcc tgatagccga cagatctcaa tgtttccaga ttacgctgag tgatcgggtt
     2821 ttgatacata ttcgtgttca tggcaggggc aatcaatttt ggtgttccca ctggaagcgc
     2881 cattgcgact gttgaaagaa gatcgtctgc aatgccgttt gctagcttgc cgataatgtt
     2941 tgctgtagca ggcgcgatga gaaacaagtc agcttgtttt gctagttcga tatgattgat
     3001 tacctgcgga tttgattctt gcatgacgtc tgtatggact gcgcgcttcg acagagattg
     3061 caaggttaat ggcgtgataa aatgtgtgct gcttttagac ataatcacat ctacctgggc
     3121 acctaatttg acgaattgac ttgtaagatc agcgcttttg taagcagaaa tacttccagt
     3181 aacgcctagt aagatctttt tcattttgat tcctccagtc taattttctc agtaatcaat
     3241 gcagcaatct cagatttact ttgagcttct tccactgttc catctttaga taagagatag
     3301 ccgtggtgtg tctcgccatg gatttccgtc aaatcatttg cgaaaataaa atcagttttg
     3361 ttatttatca gactattttt tgcgactgcc agaagagatt cctttggtac atctactaat
     3421 agtttgaaac caatgatttt tgcttttggc tgccattcac gtagataaga aatgattttt
     3481 ggtgttttct tcaaaacgag cacaagatgc tcggtgttag aagagatctt tgaaacagtg
     3541 gtatctgtct gttcattttc cgcaaaccaa ttgtccagtt cttcttgcgt tgaagaagtg
     3601 ggtaggtttt ttgccagctg ttcttctgaa aaactaaaag caggagtaaa atcactgact
     3661 gccatactgt ggataatcgc atcgtagtgt tcttttttcg ttagttcttc taattgtaaa
     3721 aataagtcca gtgcagattc gatcgtataa atcgttatat tggagtgtct tttcggtttc
     3781 agtgcttgtt tcgtagtcac gtaatcgatt gtggtatccg gattggcagc taaataatca
     3841 gctaatgctt gccctaatcg tcctgtcgaa tgatttgtaa tagcacgtac ttgatcgatt
     3901 ttttcagtcg ttcctccagc tgtgattaga atcttcattt atggtcacct acatttacta
     3961 gtgttcttcc ttggtgtgtt ccagcaagta gtttagatgc tgtttcaggc aattgatcca
     4021 gagtaatctc ttggacgacc agttcatctg caatctgcaa atccgccatc tgttgccata
     4081 aagaaagacg ttgatctatt ggtacattga cagaatcaat tccaatcaac tggatgtttc
     4141 gcagaataaa aggcaagaca gtcgcgttta gtttgattcc cccagcattg ccacataaag
     4201 ctactgcacc gttataagag atcaatggaa gaagactgct tagctgctcg ccacctactg
     4261 tgtcgatgac atagtcgatt tgctgttttc ctaaagcttt tgttgtctct ggcagaaatt
     4321 catcaggtcg tacgacttgc aaagcaccta gactttttag ccatgtgaca gactcttctt
     4381 ttctggaaaa ggcaatgatt gactggaatc ctagtttatg taacagggct atcgctgtac
     4441 tgcctacacc gccagaagca ccagtaacga caactttttt atcggaggtc ataccttgac
     4501 ggattaaagc gttcacacac aacatagcag taaaccctgc tgtaccaagg atcatcgctt
     4561 gacgcagcgt catatttttt ggtagaggaa ctaaccagtc tcctggtact ttttgatatt
     4621 ggctataacc gcctggatgg gtgacgccaa gcccaaaacc agtagcaagt acaagatctt
     4681 cttttgaaaa acgtggattg ttactttgga cgacagttcc ggataaatca atccctggag
     4741 tcatcggata tgttcgtatc acctgaccag actcgctcat tgccaatgca tctttataat
     4801 tgacatcaga ataggcaatt tttaccaaga cttcattttc agccaactca gtaaatggca
     4861 gatcactgat tgctgtatgg aaaacaggtt cctttattac ctgaaaagat cgaaaagcgt
     4921 tcattcgtga gacactcctt ttaaaatttt cttttatcat accataaagc ctactctttt
     4981 attcttgttc ttccgtaaat ttagacaaaa attaggtgaa aaataaagaa tcaaacaact
     5041 agaatttatt ccaaaagata aagaaatatt cagcaaaaaa aattttacaa agggttgcct
     5101 ttttcaataa atttcgtata ctaatacgag tggtagtaaa caggaagttt actattagaa
     5161 aacttcgatt cggaggaaat tatggaaatt ggtgagaagt taagaaattt gcgcatccaa
     5221 aagaatttga cacaagaaga actaggcgaa cgcacagatt tgtcaaaagg gtatatttcc
     5281 cagttggaac gagatttaag ttcaccatca atggaaacat tcttttctat tcttgaagtc
     5341 ttaggtgtga ctccagaaga attttttcat caagaagcag cgaatctgca agtggtttat
     5401 tcaaaagaag atcatacggt ttatttcgat gaagaaaacg ggtatgaatt ggaatggctc
     5461 gtggctgatt cgaatgaaaa agaaatggag cctgtcttgc tgacttttga aggtgcagga
     5521 gaatataaga cgtttgaacc ttcattatct gaaacgttta tttttgtatt agaaggggaa
     5581 ttagagttaa gcttaggaga gacggtctat gtggcaaaag caggacagtc gatctattat
     5641 caagcgacaa aacaacatca attaaggaat catctcaaaa aacgaacaaa agtattgatt
     5701 gttgcaacgc aatcatattt gtaatatagg gggtaaaaac gtggggaaac caatcatttc
     5761 atttgatcaa gtagtgaaac gctatgatga tgaaacagta ttaaaaaaag taagttttga
     5821 gatcgaacaa ggaaaatttt atacactgct tggtccttcc ggctgtggta aaacaacgat
     5881 cctacgtatt atagctggat tcaccgaagc tacagaaggg gacatctatt ttgaagggaa
     5941 acggttgaat gatttgccag ctaataagcg acaagtaaat acagtcttcc aagattatgc
     6001 actttttcct catatgaatg tttttgataa cgttgctttt ggattgaaga tcaagaaaat
     6061 gtccaaatca gatatcgaaa agaaggtaaa agatgctttg cgtatggtcc agcttcctgg
     6121 atacgaaaaa cgggagatta gtgaaatgtc tggtggtcaa agacagcgtg tagcgattgc
     6181 acgagcaatt gtaaatgaac cgaaagtatt gctattagat gaaccgttat cggcgcttga
     6241 tttgaaacta cgtacagata tgcagtatga actgcgggat ctgcaacaac gattaggaat
     6301 cacgtttatt tttgttactc atgaccaaga agaagcgttg gcaatgagtg atgagatttt
     6361 tgtcatgaat aaagggaaaa tcgtccaaag tggtacaccc gtagatattt atgatgagcc
     6421 aatcaatcat ttcgttgctg attttgttgg ggagagcaat atcgtggacg gtgtaatgat
     6481 cgaagataat ctagttgagt ttgttggtaa gcaatttgaa tgtgtcgatg gcgggatgcg
     6541 tctaaatgaa ccagttgaag ttgttttacg cccagaggat ttgacgatca cgacaccaga
     6601 taaaggaaaa ctagtcgtaa cggtcgatac tcagctgttt cgtggtgtcc attatgaaat
     6661 catctgttat gatgaacagc aaaatgaatg gatggtccat tcaacgaaaa aagctaaaga
     6721 aggaagcaaa gtaggattgg cctttgagcc agaagatatc catgtcatgc gtttcaacga
     6781 gtctgaagaa gaattcgatg cacgtcttga cagctacgaa gaataggggg gacaagaatg
     6841 aaaacaatgc gcaggatcta ttccgtccct tatattttgt ggctcggttt attcgtgatc
     6901 gcgcctgtat taatgatcat ttatcaatct ttttttgata tgaatgggca attcacgctg
     6961 aataattatc tatcttattt tacgtcgggg acatcgcttt ctatgacgat caattctgta
     7021 tggtatgcat ttttgatcac gtttttcacg ctgatcatca gttacccgac tgcttatttt
     7081 ttaacaaaac tgaagcacaa gcaattatgg ctgatgctga ttattttgcc tacatgggtg
     7141 aatctgctgc ttaaagctta cgcatttatt gggattttca gtgtgcacgg cagcgtgaat
     7201 catttctttg attttatcgg gatcgggccg aaacagatat tattcacaga cttcagtttt
     7261 ctgttcgtgg caacgtatat cgagattcca tttatgatta tgccaatttt caatgcattg
     7321 gaagagatga atccttcttt gatcagtgct agtagagatt taggcgcgaa tagctttgag
     7381 accttccgac gagtgatttt tccgttatca ttaaatggtg tgaaaagtgg tgtccaagca
     7441 gtatttattc cttctctttc tttattcatg ctgactcgtc tgattggcgg aaatcgggtg
     7501 atcactttag gaacggctat cgaacagcat ttcttggtta cacaaaactg ggggatgggt
     7561 tctacaatcg gtgtgatttt aatcattgct atgttcatcg tgatgctcct gacaggcgag
     7621 aagaaaaaag gaggtcgctc ataaatgaaa aaatttaaat ggtcgtatct ttatttaggg
     7681 atcgtctttt tattcttata tgcaccgatc ttctatctga ttttttattc cttcaatgaa
     7741 ggaggaacga tgaacagctt tacaggattt acttgggaaa attatgaagc tgtttttgaa
     7801 gatacacgtt tgatcattat cgtattgaat acgttcatgc tggctttctt atctgcattg
     7861 atcgccacga tcatcgggac atttggggct atgggaatct attacacgaa aaaacgacgt
     7921 gcacggacag cgttgttgag catgaataac atcttattag tctctcctga tgttattatc
     7981 ggtgctagtt tcttgatttt ctttacacta ctaggattcg gattaggttt tcagagtgta
     8041 ttgctttccc atattgcttt cagcattcca atcgtcgtct tgatggtctt gccaaaattg
     8101 caggaaatga acgattcaat ggtcgatgcg gctagagacc ttggtgctaa taatttccaa
     8161 gtagtcaaaa atatcatttt gccttatctt tctccaggaa tcatagctgg atattttatg
     8221 gcatttacct attctcttga tgattttgcg gttacatttt tcgtcacagg aaatggcttt
     8281 acgacgctct cagtagagat ctattctcgt gcaagacaag gaatcagttt agaaatcaat
     8341 gcgctaagtg cgttagtttt cttgttctcc atgatattgg tcattggata ctactttatt
     8401 agtaaagaaa accgtccaaa acggctgaag aaaaacagaa gagtacgcga gggggtggca
     8461 cggctgcgat gaaaaaatta caatctctgt ttttaggtat tctcatcatt attgcggtgc
     8521 tgctgcttgg tgttcgtcaa cttgaaaaaa caagtggcat gagtggagca aaggtactta
     8581 ccatctataa ctggggagat tatatcgatc ctagtttgat cacgaaattt gaaaaagaat
     8641 atggatacaa agtgaattat gaaacatttg attctaatga agcgatgttc acaaaaatcc
     8701 agcaaggtgg gacaaactac gacatcgcta taccaagtga atatatgatc caaaaaatga
     8761 tcaaagaaaa actagtactt ccattagatc attcgaagat caaaggatta aataatattg
     8821 atccacgatt tttagacttg gattttgatc cagataatca gtattcgatt ccatattttt
     8881 ggggaacttt agggatcgta tataacgata aattctttga tgcaaatcaa attaagcatt
     8941 gggatgattt gtggaagccg gaattaaaag acagtttgat gctgattgac ggtgcacgag
     9001 aagttatggg gctttctttg aatagtttag gctattcttt gaatagtaag aatatggcac
     9061 agctgacaga agcagcaaat aaattgaatc atttaacacc aaatgtcaaa gcaatcgtgg
     9121 cagatgagat caaaatgtat atgatcaatg aagaagcctc cgtggcagtg acattttctg
     9181 gcgaagctgc cgatatgatg tgggaaaatg aacatttaca ttatgtgatc ccaccagaag
     9241 gatccaatct ttggtttgat aatatcgtta tgccaaaaac cgtgaaaaat aaagaaggtg
     9301 cctatgattt catcaacttt atgctagaac cggaaaatgc ggcacaaaat gcagagtata
     9361 ttggttattc tacaccaaat gaagaagcaa aagcgttatt gccaaaagac atttcgtcgg
     9421 acgaacaatt ctatccatct gatgacacga tctcccatct tgaagtctac aaagatctag
     9481 gatcgaaata cctaggcatc tataatgacc tattcttaga attcaagatg tataggagat
     9541 aatggcaaaa ctcagtaaga atggggactt atcaacgttc taaagagcta aaaatcgtcc
     9601 tttttctatc tgagttttcc aatcttctac caaaagcgga tgaaacgccg ctttttttgt
     9661 gttattttat tttttctacc acgtttttct accagagaaa agtggacaaa accattgtaa
     9721 aatagggccc gagacgggct agtaaatcta agattgaaaa agttacagaa ggataaagta
     9781 attggaaact atgcaaatat acttaaccct tctgtaatct attaataaaa ctaatatatg
     9841 aatccagttc aatatttgta gctatgaaaa aacataaaaa tcatattaga taaaaactaa
     9901 attggtagat aaattatttt gtcagatatc tgaggcaaat attcgactat ttgtgtttca
     9961 aagtaaaaat tggaatacaa atatagaata gacatattgc agccgatagt ttattatgct
    10021 tttagacaca ttaaactgga gga
//
LOCUS       DAZKSU010000091         9955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000091 DAZKSU010000000
VERSION     DAZKSU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.092"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..439)
                     /locus_tag="ACG8Z3_002417"
     CDS             complement(<1..439)
                     /locus_tag="ACG8Z3_002417"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0824679.1"
                     /translation="MEEFGIDSISRTEALDFQADYEYELDQITMIPEIHQLLLALHKN
                     RIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGAAENSYEGNVKNLQSY
                     QNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRHK"
     gene            complement(662..1429)
                     /locus_tag="ACG8Z3_002418"
     CDS             complement(662..1429)
                     /locus_tag="ACG8Z3_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0824680.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1917..2342
                     /gene="lacA"
                     /locus_tag="ACG8Z3_002419"
     CDS             1917..2342
                     /gene="lacA"
                     /locus_tag="ACG8Z3_002419"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFQ0824681.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2359..2874
                     /gene="lacB"
                     /locus_tag="ACG8Z3_002420"
     CDS             2359..2874
                     /gene="lacB"
                     /locus_tag="ACG8Z3_002420"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFQ0824682.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2885..3817
                     /gene="lacC"
                     /locus_tag="ACG8Z3_002421"
     CDS             2885..3817
                     /gene="lacC"
                     /locus_tag="ACG8Z3_002421"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFQ0824683.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3820..4800
                     /gene="lacD"
                     /locus_tag="ACG8Z3_002422"
     CDS             3820..4800
                     /gene="lacD"
                     /locus_tag="ACG8Z3_002422"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0824684.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4828..5145
                     /locus_tag="ACG8Z3_002423"
     CDS             4828..5145
                     /locus_tag="ACG8Z3_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0824685.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5145..6851
                     /locus_tag="ACG8Z3_002424"
     CDS             5145..6851
                     /locus_tag="ACG8Z3_002424"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0824686.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            6993..8399
                     /gene="lacG"
                     /locus_tag="ACG8Z3_002425"
     CDS             6993..8399
                     /gene="lacG"
                     /locus_tag="ACG8Z3_002425"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0824687.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8422..8694
                     /locus_tag="ACG8Z3_002426"
     CDS             8422..8694
                     /locus_tag="ACG8Z3_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HFQ0824688.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            8715..9614
                     /locus_tag="ACG8Z3_002427"
     CDS             8715..9614
                     /locus_tag="ACG8Z3_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0824689.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3138 a         1678 c         1996 g         3143 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagtagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttc gtgtatttag gaaaagcaac atcagaccaa catctgaaac gtttgtaaat
      541 gttatctatt tgttggatat caatcgttgg aaaacatttc tcaacgctac ttttaaaggg
      601 gagttgatgg tcataaagag tgtcatcaag gtcaaaaaca atatttttat aagtcataat
      661 tctagtccgc ttttaaaata gtagtgtact ggctaaattc ctctaagtgt tgagggctaa
      721 tctggttatc tgtaatgatg gtatcgagtt gatctagatt atagaagtta aagaaatcgt
      781 atcgatcgaa tttagtactg tctactaata agaatttttc tacagcattg tttagggcaa
      841 gttgttgaat cacaccttcc ttgtcactat atgtagcaat agaattatgg gtaacagcat
      901 ttgcactaac aaaagctttg gcaaatctaa ttgcttttaa atttgtcgaa gccattgaac
      961 ctacaaaagc tccagttatt tctctatatt caccgccaag aagcaataaa tcaatggttt
     1021 cgctatcatt tagaatcaag aacacaggga gactatttgt aatgacacga attttataac
     1081 ctttacgacc tttcaattct aatgccagtt gttcaagtgt agttccgggt ccaataaaaa
     1141 tagtatcgcc atctttgatt aaagagcagg cttttttagc aatgtcaatt ttttcttttg
     1201 tatttagact tttcttctcg atatgtgtct tttcaagtgg ctttttagag gaaagtaatt
     1261 gtgcaccacc atgagtacgt gttaaaagtc catcagcttc tagagcatca aggtctctac
     1321 gggctgtcat atcggaaata tctagttcat ctattatttc ttttatggtt atggtcccat
     1381 caatctttaa catatctaaa attttttcta atcgtcgttt tttgttcata tgaagaccct
     1441 tctttcataa agtaattttt ttccaaagat aattctcttt taattgtatc ataaaagaca
     1501 atattttcaa ggtaaaacaa acaatttcaa acaaaaacaa atgttagatg atgaaataag
     1561 aacagtggat tgacgtatat tagcttaggt cagattttgt ataagacgaa aataaagtag
     1621 gacctcttaa tcagtaagtt atagaaagta aaagactttt gtaatacttg aatagatatt
     1681 tcacgtccat tttgtgatgg attaaatgaa caaaaatgaa caataattta acggtgttat
     1741 ctatttttta aaaaaacaaa taaaaaaaca aaaaataaac aaaaaagtgt tgctttttgt
     1801 ttgaatgctt gatattatat aaacaaagaa atgatgaaaa cgttttttcg aacattttgc
     1861 aaaatgtttt ctacttctac gtagcatttc tttctaaaat ttaggaggtg gtcaaaatgg
     1921 ctattgttgt tggtgcagat ctcaaaggta cgagattaaa agatgttgtg aaaaattttc
     1981 ttgtagaaga aggttttgag gtaattgatg tgactaagga cggacaagat tttgttgatg
     2041 ttaccttggc agttgcctct gaagtaaata aagatgagca aaacttaggt attgtaattg
     2101 atgcttatgg agctggtcca ttcatggtag caactaaaat taaaggtatg gttgcagcag
     2161 aagtttcaga tgaacgctca gcttatatga cacgtggaca taacaattca cgaatgatta
     2221 cggttggcgc tgaaatcgtc ggtgatgagc ttgctaaaaa catcgctaaa ggtttcgtca
     2281 atggtaaata tgatggcgga cgtcatcaaa tccgagtaga tatgcttaac aaaatgtgtt
     2341 aaggaaagga gttaaggaat gagaatcgca attggatgtg accacattgt cacagatgaa
     2401 aaaatggcag tatcagaatt cttgaaatca aaaggacatg aggtccttga tttcgggaca
     2461 tatgaccacg cacgtactca ctaccctatc tatggtaaaa aagtcggtga agcagtagta
     2521 agtggtcaag cagacttggg agtatgtatc tgtggtacag gtgtcggcat taacaatgca
     2581 gtcaataaag ttccaggtgt tcgctcagcg ttggttcgtg atatgacatc agctatatat
     2641 gcaaaagaag agttaaatgc gaatgttatc ggtttcggtg gaaagattac tggtggtctt
     2701 cttatgaatg acatcattga agctttcatt gaagctgagt acaaaccaac agaagaaaat
     2761 aaaaaattga ttgcaaaaat cgaacacgtt gaaacacata atgcacatca agcagatgaa
     2821 gatttcttta cagaattcct tgaaagatgg gaccgtggtg actaccacga ttaaggtata
     2881 acaaatgatt ctgacagtca cactaaatcc ttcagtagac atctcttatc ctctagaaac
     2941 attgaaaatt gatacagtga atagggtgaa agatgttagt aagacagctg gtggaaaagg
     3001 cttaaatgtt actagagtct tgtatgaatc tggtgataaa gttactgcta caggtttctt
     3061 aggtggtaaa attggtgaat ttattgaaag cgaattagaa caatctcctg ttagtccagc
     3121 tttttataaa atctcaggaa acactagaaa ctgtattgct atcttacacg agggtaacca
     3181 aacagaaatt ttggaacaag gaccaacagt ttctcatgaa gaagcagaag gttttcttga
     3241 ccactatagc aatcttatta aacaatcaga agttgtgaca atttcaggaa gtttaccttc
     3301 aggacttccc aacgattatt atgaaaaact tatccaactt gcttcggatg aaggagtagc
     3361 agtcgttttg gattgttcag gtgcacctct tgaaacagtt ctaaaatcgt cggctaaacc
     3421 tactgccatc aaacctaata atgaggaact ttcacagttg cttggaaaag aagtaacaaa
     3481 agatattgaa gaactcaaag acgttcttaa agagtcactt tttagtggta tcgaatggat
     3541 agtggtctca ttaggtcgaa acggtgcctt tgcaaaacat ggtgatgttt tctataaggt
     3601 agatattcct gatatcccag ttgttaatcc agtcggatca ggggactcaa cggtagctgg
     3661 tattgcatca gctttaaata gtaaaaaaag tgacgctgac ttattaaaac atgcgatgac
     3721 attgggtatg ttaaatgctc aagaaacaat gacagggcat gtgaatatga ctaattacga
     3781 aacactaaac tcacaaattg gagtaaaaga ggtataaaaa tggtacttac agaacagaaa
     3841 cgcaaatctt tggaaaaaat ttcagataaa aacggtgtta tctcagcttt ggcatttgac
     3901 caacgtggtg ctttgaaacg tttgatggca cagtatcaag atacagaacc aactgtagct
     3961 caaatggaag aactaaaggt tttggttgct gatgaactta caaaatacgc ttcttcaatg
     4021 ttgcttgacc ctgaatatgg acttccagca acaaaagcat tggataaaga agcaggtctt
     4081 cttcttgcct atgaaaaaac aggttatgat acatcaagta caaaacgttt gcctgactgc
     4141 ttggatgttt ggtctgcaaa acgtatcaaa gagcaaggcg ctgatgccgt taagttcttg
     4201 ctttactatg atgtagacag ctcagatgaa cttaaccaac aaaaacaagc ttatattgaa
     4261 cgtgttggtt ctgaatgtgt cgctgaagat attcctttct tcttggaaat ccttgcttat
     4321 gatgaagaaa tctcagatgc aggttctgtt gaatatgcaa aagtgaaacc tcgcaaagtc
     4381 attgaagcga tgaaagtttt ctctgatcca cgctttaaca ttgatgttct taaagtagaa
     4441 gtaccagtta acgttaaata tgtcgaaggt tttgctgatg gtgaagtggt ttacagtaaa
     4501 gctgaagctg ctgacttctt taaagcacaa gaagaagcga ctaaccttcc atacatctac
     4561 ctaagtgcag gtgtatctgc taaattgttc caagaaacat tgcaatttgc tcacgactca
     4621 ggtgccaagt ttaatggtgt gctttgtgga cgtgctactt gggcaggatc tgtagaacct
     4681 tacatcaaag aaggtgaaaa agcagcacgt gaatggttgc gtactactgg attcgaaaat
     4741 attgatgaac ttaacaaagt tcttgttaaa acagctagtc catggactga taaagtatag
     4801 taaaaacata aaacggagga tattgttatg aacagagaag agatgactct cttagggttt
     4861 gaaattgttg cttatgctgg agatgctcgc tctaaacttt tagaagcgct taaagcggct
     4921 gaaaatggtg atttcgctaa ggcagatagt cttgtagtag aagcaggaag ctgtattgca
     4981 gaggctcaca attctcagac agctatgttg gctcgagaag cttctgggga ggaacttcca
     5041 tacagcgtta ccatgatgca tggtcaggac cacttaatga ctactgtctt attaaaagat
     5101 gtgattcatc acctcatcga actttataaa agaggagcaa aataatgcat aaactcattg
     5161 aacttattga gaaagggaaa ccgttctttg agaaaatctc tcgaaacatc tatcttcgtg
     5221 ctattcgtga tggatttatc gctggtatgc cagtcatcct tttctcatct atctttatcc
     5281 ttattgccta tgtcccaaat gcttggggat tccactggtc aaaagacatt gagaccttct
     5341 tgatgactcc ttatagctat tcgatgggta tcctagcatt ctttgttggt ggtactaccg
     5401 ctaaagcttt gacggactca atgaaccgtg acctacctgc gaccaatcag attaacttct
     5461 tatctacgat gctagcgtct atggtagggt tccttttgat ggcagctgag cctgcaaaag
     5521 aaggaggctt cttgacagcc ttcacgggaa caaaaggtct tttgacagca tttattgcag
     5581 cctttgtgac agttaatgtt tacaaggtct gtgtgaaaaa taatgttact attcgtatgc
     5641 ctgaagaggt tccaccaaat atctctcaag tatttaaaga cttgattcca ttcactgtag
     5701 cggttgttct cctttatgga tttgaactca ttgttaaggg aactcttggt gtaaccgttg
     5761 cagaatcaat cggtaccgtt cttgctcctc tcttctcagc tgcagatggt taccttggaa
     5821 ttacacttat ctttggtgct tatgccttct tctggtttgt tggtatccac ggtccttcaa
     5881 ttgtcgaacc agcaatcgct gctatcactt atgccaatat cgatgccaac ttgcatctta
     5941 tccaagctgg acaacatgca gataaagtta tcacttctgg tactcaaatg tttattgtta
     6001 ccatgggtgg aacaggagct acattgattg ttccattctt gttcatgtgg atttgtaaat
     6061 cagaacgtaa ccgtgccatc ggacgtgcct cagttgttcc aacattcttt ggggttaatg
     6121 agccaatctt atttggtgca ccaatcgtat tgaatccgat tttctttgta ccgttcattt
     6181 tcgctccgat tgtcaacgtt tggatcttta aattctttgt tgacacattg aacatgaact
     6241 cattctctgc caaccttcca tgggttactc ctggtccgtt gggaattgta ctcggtacta
     6301 acttccaagt tctatcattt atcttagccg gactcttggt agttgttgat actatcattt
     6361 attacccatt tgttaaggta tacgatgaac aaattcttga agaagaacgt tctggtaaaa
     6421 caaacgatgc tcttaaagaa aaagtagctg caaacttcaa tactgctaaa gcagatgccg
     6481 ttcttggaaa agcaggcgtt gctaaagaag atgtagcagc aaacaataat atcacaaaag
     6541 aaactaatgt ccttgttctt tgtgcaggtg gaggtacaag tggcttgctt gccaatgcct
     6601 tgaacaaagc agcagcagaa tacaatgttc cagtcaaagc ggcagcgggt agttacggtg
     6661 ctcaccgtga aatgttgcca gagtttgact tggttatctt ggctccacag gtcgcttcaa
     6721 acttcgatga tatgaaggct gaaacggata aattgggcat taaacttgca aaaactgaag
     6781 gagctcaata tatcaaattg acacgtgatg gacaaggtgc tcttgcattt gttcaacaac
     6841 agtttgatta ataaatttgt aaaagttatt tgagacgaga ttattctttc agttccttat
     6901 aatattgtta accatatcat tgcatttcat ttccttggtt tccggaactc agaatatcgt
     6961 ctcaaatgct ttttttgaaa ggatttatag ttatgactaa aacacttcct aaagatttta
     7021 tttttggtgg tgctacagct gcttatcaag cagaaggtgc gactcacacg gatggtaaag
     7081 gcccagttgc atgggataag tatcttgaag acaattattg gtacacagca gagcctgcaa
     7141 gtgattttta ccataaatat cctgttgatt tagaattagc tgaaaaatat ggcgttaatg
     7201 gtattcgtat ttcaattgcc tggtctcgta ttttcccaac tggctatggc gaagtaaatg
     7261 aaaaaggtgt tgaattctac cataatcttt ttgccgagtg ccacaaacgt catgttgagc
     7321 cattcgtgac tttgcaccac tttgacacac cagaagttct tcactcagat ggagatttct
     7381 taaaccgcga aaatatagaa cactttgtag attatgccgc tttctgtttt gaagagttcc
     7441 cagaagtaaa ctactggaca actttcaatg aaattggccc aattggagat ggtcaatact
     7501 tggttggtaa attccctcca ggcattcaat atgatcttgc caaagtcttc caatcacacc
     7561 acaatatgat ggtttctcat gcacgtgctg tgaaattgta taaagataaa ggttataaag
     7621 gcgaaattgg tgtagtacat gcgttgccaa ccaaataccc ttatgatcca gaaaatccag
     7681 cagatgttcg tgccgctgag ttagaagata tcatccataa caaatttatc ttggatgcaa
     7741 cctaccttgg acactattca gataaaacga tggaaggggt caaccacatc ctagctgaaa
     7801 atggtggaga acttgatctt cgtgatgaag acttccaagc acttgaagca gctaaagatt
     7861 tgaacgattt ccttggtatc aactactaca tgagtgattg gatgcaagcc tttgatggtg
     7921 agactgaaat cattcacaat ggtaagggtg aaaaaggaag ttctaagtac cagattaaag
     7981 gtgttggtcg ccgaatcgca ccagattatg tgcctagaac ggattgggac tggattattt
     8041 atcctgaagg cttgtatgac caaatcatgc gagtgaaaaa tgattatcca aactacaaga
     8101 agatttacat cactgaaaac ggtcttggat acaaagatga gtttgtagat aataccgttt
     8161 atgatgatgg tcgtattgat tacgtgaaga aacacttgga agttttatca gacgcaattg
     8221 cggatggtgc aaatgttaaa ggttacttca tctggtcact tatggatgtc ttctcatggt
     8281 caaacggata cgaaaaacgt tatggactat tctatgtaga ctttgacacg caagaacgct
     8341 atcctaagaa atcagctcat tggtataaga aagtagcaga aactcaagtg atagagtaat
     8401 attacttgaa aatgtaaaaa attgttatcc gatactttag aaaaagtaca acacttattt
     8461 agaccaggct ttgaaaaggt atatattgtt agtttcgaag attgtcctat gattcctgag
     8521 cttgaagcta ccccgttgct aaaatgtggt aaatggtatg tttcaactgg aaaagagtgg
     8581 atttgccatt ctgatttaga gttatcagca tttgaatggg aatttttaca atcactcgat
     8641 gtagaaatac gtgagactat tcacttcgag gtaaattatt tgcctttcca ataataaaaa
     8701 gaaaggaatt agttgtgact atcgaattaa aaaacgagta tcttactgtt cagtttaaaa
     8761 cacttggtgg tcaactgact tcgattaaag acaaggatgg gatagagtat ctctggcaag
     8821 ctgatccaaa ttattggaat ggacaggcgc ctattctatt tcctatctgt ggtagtttac
     8881 gaaatgattg ggctatttat agacctcaag aaagaccttt ttttacaggt cttattcgta
     8941 gacatggttt tgttcgaaaa gaagaattta ttctggaaga agtcaatgat aatagcgtga
     9001 cctttagtat taaaccaaat gctgagatgc ttgataatta cctatatcag tttgaactga
     9061 gagttgttta taccttgaat ggtaaatcaa ttagaaccga atttcaggta acgaacttgg
     9121 aaatggaaaa aacaatgcct tactttattg gagcccatcc agcattcaat agtcctttag
     9181 tagaaggtga aaactatgag gattattata ttgaatttag tgaggttgag tcttgctcga
     9241 taccaaagag ttttcctgag acaggattat tagatttgca agatcggaca ccttttttgg
     9301 aaaatcaaaa aattctcgat ttggactact cacttttttc ccatgatgcc attacacttg
     9361 accagctcaa gtctcgtagc gtaacgcttc ggtcaaggaa gtctggtaaa ggtcttcgag
     9421 tagattttga tgattttccg aacttgattc tgtggtctac aactaataaa agtccattca
     9481 ttgctttgga accttggagt ggtctctcca cttcacttga agaaggtaat ttcttagaag
     9541 ataaaagaca ggtaacgaaa atttcacctc aagaaacttc tagaaaaatc tatgatatta
     9601 ctatccttaa ttaaaatttg ttattatttg agtcttcaat aagatttgaa tactcttacc
     9661 ttgtgatgat agattggtct tctccgtgcc agtcttacat cgcaaggttt ttaagttttt
     9721 tgtgggtcta tgtcaactgt agtgggtgac gaaaagctaa tatctaaaga cagtctggta
     9781 gtaaaaaata agattattta ctacttcttg atttaaaata atagtggagc cattaatttg
     9841 tggcaaattt aatttaatat tttgcctcaa attagccaca ctttttatag aactaagagg
     9901 aaaaatgaaa ttttcttcct attaatatag ttgtttgaaa tcttaaatat agcgt
//
LOCUS       DAZKSU010000092         9926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000092 DAZKSU010000000
VERSION     DAZKSU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.093"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..359
                     /locus_tag="ACG8Z3_002428"
     CDS             3..359
                     /locus_tag="ACG8Z3_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HFQ0824690.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            375..725
                     /locus_tag="ACG8Z3_002429"
     CDS             375..725
                     /locus_tag="ACG8Z3_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HFQ0824691.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            754..1533
                     /locus_tag="ACG8Z3_002430"
     CDS             754..1533
                     /locus_tag="ACG8Z3_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HFQ0824692.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1544..2221
                     /locus_tag="ACG8Z3_002431"
     CDS             1544..2221
                     /locus_tag="ACG8Z3_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HFQ0824693.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2240..3346
                     /locus_tag="ACG8Z3_002432"
     CDS             2240..3346
                     /locus_tag="ACG8Z3_002432"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFQ0824694.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3336..4427
                     /locus_tag="ACG8Z3_002433"
     CDS             3336..4427
                     /locus_tag="ACG8Z3_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFQ0824695.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4440..5180
                     /locus_tag="ACG8Z3_002434"
     CDS             4440..5180
                     /locus_tag="ACG8Z3_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFQ0824696.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5468..7408
                     /locus_tag="ACG8Z3_002435"
     CDS             5468..7408
                     /locus_tag="ACG8Z3_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0824697.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7495..9417
                     /locus_tag="ACG8Z3_002436"
     CDS             7495..9417
                     /locus_tag="ACG8Z3_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0824698.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9697..>9926)
                     /locus_tag="ACG8Z3_002437"
     CDS             complement(9697..>9926)
                     /locus_tag="ACG8Z3_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFQ0824699.1"
                     /translation="IDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAV
                     GSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3236 a         1648 c         2205 g         2837 t
ORIGIN      
        1 tcatgattaa agtagttatt gcagaccgta tgggtaaagg acaaaatgtg gccaaagggg
       61 tagaagcagc tggtggaaaa gcagttgtcg taccaggaat gggggcagat atgcgtttgg
      121 gcgatgtgat gcaacaagag catgcagata tgggtatctc tttttgtggt agtggtggtg
      181 caggtgcatt aacagcagca acaaaatatg gctatccaga acgacatggt atgcgttcga
      241 ttgaagaagg gatcacagct atcaaggatg gcaaaactgt tctaggtttc ggctttatgg
      301 atcaagagga attagggaaa cgtctgacag aagaatttat caaaaagcat ggaaaataga
      361 aagagggaca tgaaatgagc gttcaaaaga caattcagca aaaagttatc gtctccggta
      421 aaggggaaac aaagcaacat gcttttgcat cagcgttagc agaaattcaa aacaaaataa
      481 tggaacaatc aaacgtatta ttgcgaatcg aaccagtcaa tgtggatatt ttaaaggcgg
      541 tcgaaaacaa atacacagaa cgctttctat ttttcttctt cccacggcaa cgtattagct
      601 atgaagtcga actctctgtt actgtggctg taacagaaat aaaattggca gacatatcgt
      661 tcagccagca aatgactgaa gatccaaatg ggattgccat tccatttgta actaaaaaaa
      721 tctaattttg aatcataagg gggaaagaat aacgtggatt tcgtaatcat tctagtcaaa
      781 tcggtcatta tcgggggatt acttggtttt gcagcatctg tcggggcagc tcgaatgttt
      841 catgcaccta atacacaagg tttaggagct tttagaacat taggcgaaat gaatgcctgc
      901 atgggtgatc cagcttctca tttttcattt ggtttaggct ttttctttaa tgcatgggct
      961 tcttcagttg gtgctggtgc attcacacaa gatgtgacgc atcgaatcat tcccaattgg
     1021 gcagcggcag cacttctagc aaaaaacaaa gatgtcaccc agaccatgca tgatccgaaa
     1081 aaaatgggga tcattggagc ggttatcggg gcagtggtgg tagccttctt aaacacaaca
     1141 gctgcagcaa ttccagaggc gttacaagtg acggcggttt ccgttttggt accagcagcg
     1201 acgattctga tcaatacagt aatgccagtt attttttggt tggcagcttt agacgcaggg
     1261 aaacgaactg gtttttgggg cactcttttt ggtggtatcg cgcaattgat tatgggaaat
     1321 gcggtacctg gtgttgttct aggaatcttg attggtaaag gcgtcgatga gagcggttgg
     1381 aatcgcgtca cgaagagcat gatgatcgct attgttttat tatttatttt cagtggattt
     1441 ttccgtggat ttgatatgga aatgatcaag agcttccagc ttcaagtgcc taattggctt
     1501 gataacttgc acaacatctt tagtgtgaaa taggaggaga atcatggaga caacagaaga
     1561 acaagcaaaa gtattagaag caaagcggca aaagagcttt tggtttgcag actggtcttt
     1621 tccgctaatc gtaggaatta tgtccgctgc tgtatttgca ggaacacaca tgtatgtgat
     1681 gtatggtgtg ggtgcattca atgaagtttc gatcgtggcg atgttaaaag ccgggctgga
     1741 tggcggatcc tatggcgcag cggcagcgtt tggtgctagt ttcttatttg ccagaatttt
     1801 agaaggctct ttagttggga tcttggactt aggcggttcg atcctaactg ggatcggaat
     1861 tggggtacca gcaattctct taagtatgaa aatagcggca ccaatcgata attttgcact
     1921 ctctttattg actggtttgg ttctaggttt gattgttggt ggcatcattg tcttgattcg
     1981 taaatttacg atcaatcaat caaattccac ttttggtgct gacgtcatga tgggggcagg
     2041 aaattcatct ggacgttttt tgggtccatt aattatatta agcgcgtgtg cagcatctat
     2101 tccaatcggc attggcgcta cattaggagc actaggattc tatgcttgga aaaaaccaat
     2161 tgctggaggt gctattttag gagcaatgat tttaggagca atcttcccgg cagtaaatta
     2221 atagaaagag ggtcattaaa tgtttgatac tttgattaaa aatggaaggt tagttgatgg
     2281 atctaaaata gaggtcgcta tagaaaaagg catgattaaa gcagttgcag cagaaataaa
     2341 tcagccggcg caagaagtag ttgacttgga gggaaagtat tatttatccg ctgggtggat
     2401 cgatgatcat gtccactgct atgaaaagat gaatctttat tatgactatc ctgatcagat
     2461 aggggtcgaa aaaggtgtga cgacggtcat tgatgctgga actacgggag cagaaaacat
     2521 ccgtgaattt tatcaattga cgaaatcggt caagacaaat gtgtatgcgc taatgaatat
     2581 ctctaaatgg ggcattgtca aacaagatga acttgctgat ctaacaaaga ttcaagaaga
     2641 tctagtaagt actgcgttag cagaactgcc ggaatttatt gtcgggatta aagcgagaat
     2701 gagtaagaca gttgttggtg aaaatggcat taagccatta gaattagcta aaaaaatcca
     2761 gacagaaaac aatgatctgc cattgatggt tcatatcggt tctgcacctc ctgagttaga
     2821 agaagtattg tcaagaatgg aaagaggcga tgtattgacc cattgtttta atggaaaacc
     2881 aaacggtatt ttagatccaa agacagatca aattaaaaac tttgtctggg atgcttacaa
     2941 taatggtgtc gtctttgata ttggacatgg gacggatagc tttaatttcc atgtggcaga
     3001 aaccgcacta aaagcaggaa tgaaagctac ctctattagt acggatatct acatccgcaa
     3061 tcgtacaaat ggcccagttt ttgatctagc aactacttta gaaaaattga gggtagtcgg
     3121 ctattcttgg gaagaaatca ttgaaaaagt tacagcagtt cctgcacgga attttcattt
     3181 gaagaataag ggccagttag ctgtcaatta tgatgcagat ttgacgatat ttgagattgt
     3241 tgaaaatgaa aaggaattga aagattccaa cggatttaca cgtatagcta aagaacaaat
     3301 tttaccgatt aaaacaatta ttggaggcgt catatatgac aattagctat ggaaagtttg
     3361 atttaaaaga agtcattaat gcatcgggaa aaatgacgat cctaggggtt tctaaggtat
     3421 ctgaaagtgt cctagcagca caacgatttg gaggcgagca tttttttgag atgagtgatc
     3481 ttgccatcaa aaccggcgct tatctttctc aattactgaa agtggaagat gcgcaaatcg
     3541 tttcttctgc ttcagcaggg attgctcaat ctgttgctgc tttaatcgga cagggcgaca
     3601 tgtaccatgt ctatcatccc tatacagaaa aaatcgctag acgggaaatc atcataccta
     3661 agggacataa tgtagattat gggacaccgg ttgaagtaat ggttgctcaa ggtggaggaa
     3721 aagtcataga agctggctat gcaaatatgt gtacaccgga acatgtttcc atgatgatta
     3781 cggaaaaaac tgctgcgctt ctatatatta aaagtcatca tgcagttcag aaaagtatgc
     3841 tgacggtggc agaaatggta gaagttgcca aagctcatcg tttgccattg atcgttgatg
     3901 cagctgcgga agaagattta ttcaaatata gcgaaatggg cgttgactta gtgatttact
     3961 ctggggcaaa agcaatcgaa ggtccgagtg ccggactggt cataggaaaa aagaagtata
     4021 ttcaatggat tcggctacag tctaaaggta ttggccgagc aatgaaaatc ggtaaagata
     4081 atattttagg ctttacccag gcactagaag attatttaaa aaatgggagc gaatccggtg
     4141 actccatgaa gcaacgatta gcgccttttg ttaacgcaat caatcaactt cctgaccttt
     4201 cagcgaaaat agttcaagat ggtgccggca gagatattta tcgagcaagt gtaacgatta
     4261 atggcgataa aagtgcgaaa gaagtcattc aggaattaaa agcacgaaat ccagcagtct
     4321 acacccgaga atatcaagca aataacggaa tcattgagtt cgacattcgt tccgtcagcc
     4381 aaaacgaaat ggaaaaaatt ataaaaagat tgagagaaat catgtaggag gcttttaaat
     4441 tgtcagttat cccaaattat tataaaaatc gtgtatgttt aaatgtatta gctcattcag
     4501 ttgaaaatgc aaaagcatgc tatgaagcag cggaagggca tgtcgtacta ggtgtgctat
     4561 cgaaaaatta tgctagcgat gaagcagcaa tcgaagatat gaaaaggtac caagacgcaa
     4621 ccaataatgc gttgtcagtt ggattaggcg ctggagatcc aaaccaaagt cagatggttt
     4681 cgcgtttatc tggggtttta caaccacaac atgtcaatca agtatttact ggcgtgggga
     4741 cttctcgtgc tttattgggc caaaatgata cagtcatcaa tggattggtt tcaccaacag
     4801 gtaagatcgg ctatgtcaat attgctacag gtccgttaag ttcacaagcg ccagcagctg
     4861 aagtgcctat tgaaactgcc attcaaatgc tgcaagacat gggaggtact tcaatcaagt
     4921 atttcccgat gaaaggctta gcacataggg aagaatttaa agcagttgcg gaagcttgcg
     4981 caaaatatga attctattta gagccaacag gaggaattga tctttcaaac tttgaagaaa
     5041 ttctgaaaat tgcaatcgat gcaggtgtaa aaaaagtgat tcctcatgtt tatagttcaa
     5101 ttatagataa agaaacagga gatacgcgta taggagacgt aaagatacta ttaagcatgg
     5161 taaaaaaaat tattcactaa ctcattagaa aataagaagc ttattccaga atgaaattaa
     5221 atggaataag ctttttataa ttaattacgg aaattgttaa tgtagataat aatttagatg
     5281 tacgtaagca gaagtacaga aatgtaagat tttccaatat tattttattc aacataataa
     5341 atcgtatatc aattttttta gcgaaatttc tatttattcc aagaatttca gttattacgg
     5401 aatcattttg tgcttaataa aaagaaaccg tttttatata ctataaatgt agaaaggcgg
     5461 taggaaaatg aaaaaaatta ttgcaatcac atcctgtcca gttggtattg cccatacata
     5521 catggcagca gaaaacttag agaaagcggg gaaagcagtt ggcgccgagg taaaagtcga
     5581 aacacatggt tccatcggta ttgaaaatga attgactgca agagatatcg aagaagcagc
     5641 tggtgtaatc atcgcagcag atactaaaat agataaatca cgattcggcg gtaaaccttt
     5701 gataaccgta ggtgtgcaag aaggaatcca ccatgcagat gagttggtga gagatatttt
     5761 agcaggaaaa gcgccagtct ataaaagtac ggaggcaatt atttcttccg aaaataaaag
     5821 tgaatcagaa aatttaggta aaaaaatcta taagagtttg atgaatggcg tttcgtacat
     5881 ggtacctttc gtagttacag gagggttatt gatcgcgatt tctttaacat taggaggaac
     5941 agctacacct gaagggatca agataccaga aggaacgatt tgggcaacga tgaatagtat
     6001 cggaagtatc gctatggggt tgatggttcc aattctatct gcatttattg cgcaaagtat
     6061 tgcagatcgt ccaggacttg ttcctggatt tgttggtggg atgttagcgg caaacggagc
     6121 attatatggt agtgatgcga atgcgggatt cttaggcggg atcatcactg gatttttagc
     6181 aggattcatt gctttaggaa tcaagaaagt gcgagtaccg aaagctttac aatcgatcat
     6241 gccaattatt gttatcccaa ttttaggatc attggcagtt ggttttgtgt ttatctatgt
     6301 tattggagaa ccagtagctc ttctatttag ttcattgact cactttttag cagggatgca
     6361 aggaggaagt gaaattgttc tagctatgat cttaggagcg atgattgctt ttgatatggg
     6421 ggggccgttc aataaagtag cctttttgtt cggatctggc ttgattgcag aaggcaatta
     6481 ttccattatg gggccaattg ctgtagctat ctgtatccct ccattagcat tggggctggc
     6541 atcattgatt ttaaaaacca aatttacgaa aacagaacga gaagctggta aggcgtcctt
     6601 agcaatggga ctgtttggga ttacagaagg cgctattccg tttgcttcaa cagatccact
     6661 acgggtgatt ccaagcattg taatcggggc aatgacagga agtgtgatcg cgatgctttc
     6721 aggagtgaca gatcatgtag ctcatggcgg tccgatcgta gcggttttag gagcagtgga
     6781 tcacgtagcc atgttttttg tagcagtgat tgctggtatt gctgtaaccg tgctaatgtt
     6841 aagaatcctt aaaccgacat tacaacaaac tgcggttgct gcagcaaatg gtgtgacagt
     6901 agcagatcta tcagagactg aaacaagtga tgtttatacg aaaactgaaa cttttgattc
     6961 tatcacggat attatgtcag atgaagcaat cgttttaggt gaaacagcaa caacaaaaga
     7021 cggagtaatc gaagatttag tccaacgttt agaaaaaaca gcggcgatca cagataaaac
     7081 cagatttaaa gaagtcatct atgatagaga acatgaaagt acaacgggaa tcggtatggg
     7141 gattgctatt ccacatggaa aatcagctta tgttcttaaa ccaacagtgg catttgccag
     7201 aagtcaatct ggggtagatt ggcattcttt agatggaaaa cctgcacata tgatctttat
     7261 gattgctgta cctgaaaaca gccaaggtga tatgcacttg aaaatactgc aacgtctatc
     7321 acgtaaattg atggatgatg acttccgtca agcactgatg gaagcgccag ataaaacaac
     7381 agttcaccaa ttattaagcg aaatgtaaca taagatgaaa aatcgaccgt gatgagacat
     7441 aatagatgtg ttatcatgat cgatttttta tttagaaggg agtttctgca cattatgaat
     7501 cagcgtcagc aatctttgat ctatcaatta agtgagaaca ataattatat aacaacaaaa
     7561 aagctagcag agcatttcca tgtgtcagaa agaacgattc atagcgattt gaataccatt
     7621 gaaacttggt tgagagacaa acatctgcct ttaatgtttg aaagaaaaaa aggtacaggt
     7681 atcttgttga atggtctgcc tcacgaaagg gaacaactga aacgagcgct taatgaagag
     7741 actcaaacag aaatggataa aaaacagctt cgtcagttat tgattttcca tctgctagta
     7801 gcaaaagacg gatttagtct tgaagaactt tcagaaaaac tatatgttgg aaaacgtacg
     7861 attcgtaaag aattgacaga gttgcaaagc ttttttgagt atcaccattt acagctagta
     7921 tccaaaacaa aactaggaac ttttcttaaa ggcgatgaac aagaaaaaag acagttgctt
     7981 gttaagacat tacgaaatat gcagaaagaa gatccacaaa gtccgtcgtt gaaagaattc
     8041 ttcgctaaag atacattaaa agttatccaa catacattac aagatgtttt ttatgaaaac
     8101 catttagaac agccgaatgg actggctagt gtggagattc acatctactt tatgctcgag
     8161 cgaatgaaac agtaccagaa agtgaaatta agtaaagacg aaaacgaagt cgtggaacac
     8221 acccaagcac aaaagttaag tagtcaaata ttagcaaaac tagccaccat ctatccaatt
     8281 gaattttctc ctgatgaaat caattatctg gctttgcgta tcgccaatct ttttacaaat
     8341 tcacaagcag ccacacgttt tcaaaaggaa tctagcgcac ttgctgatca tttgatcgaa
     8401 caagtggaac agtttctagg ctattcatta aaagaagatc agctactaaa acaaaattta
     8461 aggtctcatt tatcttcaac ttattttcga ctccattatg gtttgcagat ttctaatcca
     8521 ttgacgaaaa atgtgtttag tacgtacaca caacttttct tagttttaca gttaatttta
     8581 gaagattatt ttgaaaagga aaatttttat gttccacaag aggagacagc ttatttaacg
     8641 atccatttcc aagcagcgat cgagcgacac aaacatcaga aaagtagaag atatcaaacg
     8701 gttttagtta gtgaatattc aaaagctatg gctacttttc tcgaagcacg gctgaacaga
     8761 gaacttccag aattaacgat tatggatcta gttgaatata agccagaatt aaagagacaa
     8821 gattttccct ctgttgattt tatcttggct acgttaccat ttaaacatat aagcattcca
     8881 gtaatcgaga tctcaccaat gatcacagaa actgatctag cctatttgac aaaatatatg
     8941 ctggagcatg tgcctatcaa gaagaaaaaa acatttgatc tagcaagctt tactcatcca
     9001 tttctgatct ttcctcaact tgaacggaca gacccagccg atattttaaa ctttatggga
     9061 aacgtcttag tggaacatca ttatgtggag ccggaattca tcgattctgt cttggaaaga
     9121 gacagtcact ccagtactag agtagcacca tttgtaacaa tccctcacgg taatccgctg
     9181 tatgtgaagc attcgatgat ttctattgct acaatgaaag aaccagtcct atggcacggg
     9241 gaacagatac gtatcgtact gctattagca ataaagaaaa atgatttgaa acatgcagag
     9301 ttcaaaaaaa tattttctgt tattcactat ctagaaaagc agccagaaca aatggctcag
     9361 ttgatgcaat ccaatcatcc gctagaaatc ttaacgttgt tatctagtta tgaataagta
     9421 gcgattaaaa aactgccttt atatttataa taattctatc tatttatttg gaagtagaaa
     9481 actaaataca aaaaaagagg atgtggccaa agttgtggca catcctttta ttttgagttt
     9541 cttataattt tttatagttg cttacgatgt cttgactact ggtaaacggt gttcaagaga
     9601 caatttgtcg gcaataaggc acaattttgt aaaatatgga aagctatttt tttaacattg
     9661 tttcttagtg cttgaaaaat cattttgaat acttctttat acgatcgatg tacgttgatt
     9721 taagtctaaa acttttcttt tgattccttc tctgactttt ctgaattccg ttaatatttc
     9781 ttcttcgctc ccgacagctt gtgcaggatc ttttaggtcc cagtgaaggt gttcgtgatt
     9841 gtttggaacg gcagggcact tgtctttggc atcaccacaa agtgtaatga tcaagtctgc
     9901 ctgagtaaag taatctctat caatca
//
LOCUS       DAZKSU010000093         9862 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000093 DAZKSU010000000
VERSION     DAZKSU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.094"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(251..1099)
                     /locus_tag="ACG8Z3_002438"
     CDS             complement(251..1099)
                     /locus_tag="ACG8Z3_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0824700.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1262..2164
                     /gene="gnd"
                     /locus_tag="ACG8Z3_002439"
     CDS             1262..2164
                     /gene="gnd"
                     /locus_tag="ACG8Z3_002439"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0824701.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2166..3167
                     /locus_tag="ACG8Z3_002440"
     CDS             2166..3167
                     /locus_tag="ACG8Z3_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0824702.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3164..3448
                     /locus_tag="ACG8Z3_002441"
     CDS             3164..3448
                     /locus_tag="ACG8Z3_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0824703.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3481..4278
                     /locus_tag="ACG8Z3_002442"
     CDS             3481..4278
                     /locus_tag="ACG8Z3_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0824704.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4292..5125
                     /locus_tag="ACG8Z3_002443"
     CDS             4292..5125
                     /locus_tag="ACG8Z3_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0824705.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5143..6000
                     /locus_tag="ACG8Z3_002444"
     CDS             5143..6000
                     /locus_tag="ACG8Z3_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0824706.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6022..6531
                     /locus_tag="ACG8Z3_002445"
     CDS             6022..6531
                     /locus_tag="ACG8Z3_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0824707.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6568..6969
                     /locus_tag="ACG8Z3_002446"
     CDS             6568..6969
                     /locus_tag="ACG8Z3_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824708.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7081..7434
                     /locus_tag="ACG8Z3_002447"
     CDS             7081..7434
                     /locus_tag="ACG8Z3_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824709.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7468..7911
                     /locus_tag="ACG8Z3_002448"
     CDS             7468..7911
                     /locus_tag="ACG8Z3_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0824710.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7928..9013
                     /gene="uxuA"
                     /locus_tag="ACG8Z3_002449"
     CDS             7928..9013
                     /gene="uxuA"
                     /locus_tag="ACG8Z3_002449"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0824711.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9006..9719
                     /locus_tag="ACG8Z3_002450"
     CDS             9006..9719
                     /locus_tag="ACG8Z3_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0824712.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         3292 a         1558 c         2171 g         2841 t
ORIGIN      
        1 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
       61 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
      121 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
      181 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
      241 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
      301 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
      361 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
      421 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
      481 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
      541 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
      601 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
      661 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
      721 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
      781 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
      841 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
      901 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
      961 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
     1021 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
     1081 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
     1141 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
     1201 taaaatctat ttacaaaaaa attttttgta taatgagggt ataagaaatg gaggcgctat
     1261 catggaaatt ggtgtaattg gtttgggaaa aatgggattg aatttagctt taaacttaaa
     1321 agagcaccat cacaaagtac aaggactcga catttcaagt acagctgtta gtcaggcaag
     1381 agaaaatgac attcctgctt atcaagagat ctctgaattt ctagctactt ttgagcagaa
     1441 aaaaatcatc tggttaatgc ttcctgctgg agagataacc gaaaacatgt taagaaaact
     1501 ttttccaaaa ctcggaccag gagacattat tattgaaggc ggaaactctt attataaaga
     1561 ttcaatccgt cgagcagaag agtttcagaa aaatgaaatc ggttacttgg attgtgggac
     1621 ttctggagga atcgaaggag cacgacatca agcctgctta atgattggag gagataaaga
     1681 aacttttcta tccgtggaac aattattcaa agatgtagca attgaaagag gatatctgta
     1741 tgctggtgca tccggaagcg gacacttctt gaaaatgatc cataatggaa tcgaatatgg
     1801 aatgatgcaa gcaatcggtg aaggattcca acttgtacaa aaaagtgagt atgcttttga
     1861 cttggaacaa gtcgcacgtg tttggaatca tggttcggtg attcgcggct ggctgatgga
     1921 aatcatggaa gatcagttaa acgaacatcc caatttaagt aattatcggg gaatcgtttc
     1981 tgcatcaggt gaagcaaaat ggacggttga aacagcgcta gaaatggctg ttccagttcc
     2041 agtaattgca actagtttgt tcatgaggaa tctttcacag gaagaagata gtttttcagc
     2101 aaaagtagtt tcagcactaa gaaatgggtt tggcggacat gaaatagttg aaaagggtga
     2161 gtaaaatgcc agattatcat atagcaatcg ttaattccag cagtttcgga agagtctttc
     2221 cacaccactt ggaaagatta aagtcagttg gagacgttca tttctttact gttgatcaag
     2281 agattccagg aaaagaacta gcggagttac ttaaaggatt caatatcatt attgcaagtg
     2341 ttacgccatt ttttcgacga gatttttttg aaaacaaaga tgaactgcta ttgatttccc
     2401 gacatggtat tggttacaac aatattgatt taaaagcagc aaaagaacac cagacaatcg
     2461 tatctatcat acctgcttta gttgaacgaa atgctgtagc agaaaataat ataactaatt
     2521 tgttgtccat gcttcgacgc acgacacaag cacaagcaag agtcaaggac gatcgatggg
     2581 aagatcgggc ggagttcgtc ggtaggacgc tgtttaacaa aacagttggt gtgattggtg
     2641 caggaaatac cggaagcgga gtaatcgaaa ttttgcgtaa cggttttcgc tgtgatgttc
     2701 tagcttatga tccttacaaa tcagatttat acatcgaaag ctttggtgca aaaaaagtag
     2761 aacttcaaga attactggaa caatcagata tcatttgttt atgtgcgaat ttgacagaag
     2821 agaactatca catgatttct gataaagaag tcgatcagat gaaagaaaat gtttatctat
     2881 ctaatagtgc aagaggcgct ctagttgatg aagaagcgat gattaatggt ctgaaaagcg
     2941 gaaagatcgc aggatatgcc acagatgttt tagaagaaga accgggacgt caagatcatc
     3001 cgtatttagc gtttgaacaa gtagtgatta cgccacatat atcggcctac acaagggaat
     3061 gtttagaagc aatgggtgaa aaatgtgtca gagatgtcga acagatcagt cagcaccttc
     3121 ttcctgaacg ttctgtccag acaaagagca actatataaa aggatgatga agaaaatggt
     3181 gaacggttta aaagtgaaaa caggccctca attttattta tatgaagaag gtggtatttc
     3241 aaaagtttct gatctcttga aatcatacgg cgcgaaaaga gtactggtca cccacggaac
     3301 ggtatcatgg gaaaaggcgc tacctaaatt agtatttttg aatgacgaaa cgattcaatt
     3361 tttttatcat cgttattctg gagaatgtag ttatgctgaa gccagacgaa tagctacaat
     3421 catcaaaaaa atgaaatcga tttcgtagtt ggcgttggcg gaggaaaact gaccgatcta
     3481 gtgggttata gtgccaatct tgcaagaatc gatttcggag tcatccctac tttagctagt
     3541 aattgtgctc catggacacc tcttgctgtc atgtataagg agaatggaca agcagaaggg
     3601 aaaacagaac acttttacag acaagctgct ttttttataa cagatcccca attagtgatt
     3661 gattcccctg ttccatattt cattgccggg ttagcagata cactagcaaa atggtatgaa
     3721 tctgagctca tcttagaaca agattttttg caagatgaat catttttgaa gctagcacaa
     3781 gatactgcga agatctgcaa agaagagatt ttgaatcatt ctgctaaagc aatccaagat
     3841 atggcacaaa gaaaactgac atcggagttc aaacagcttt cagagattat ctttgctgtt
     3901 gctggtttag ttggaggatt aggagataaa tacgcaagaa atgtggcagc acatgctatg
     3961 catgacgcta tcagtaaata tattccggaa agccatcgtt ttctacatgg agaaaaagta
     4021 gcgtatggga ttttctatca actggcacta gaaggtcgct gggaaaaaat agacgagtta
     4081 tatcctttgt atcgggaatt agaactgccg gtttctctag aacaaatggg attatttccg
     4141 aaagataaac agataataga taaaatggct gaatttattg attcgaaaga gaaagtgcat
     4201 ttgattccta tagaggtaac acagaaatca ctgatacagt caatgatgga actggaaggt
     4261 tatatcagaa agatctgaag gaggaggaca catggaaaca ttaagtgtag tacaaagttt
     4321 gttgattgct ttatgggtag ccgcggttat gtcccgctgg ttaggtggag gagccacgtt
     4381 gaccttacgt ttttctcctt tgatgactgg attagtcgtc gggttgatca tgggggaagt
     4441 tgcacaagcg atgattgtta cagccgcttt gcaaatgatt tatatggggg ttttttctcc
     4501 aggaggatca atgccagctg aaccatctat tgcagcagct atcgcagtac cagtcgcatt
     4561 attaggggat ctgtcgccag aagcagcgat tgccgttgcg gtgccagtcg gattgctcgg
     4621 aagttacttg tatcagtttc gttttttcat caatactttt ttaggtaaat atacagacaa
     4681 agctgtagaa gatttgaata caaaagctat ggcccgatca attattcttt atccaacggt
     4741 tgcctcattt ttattattcg ttccgcttgt tttcattgct ttatatttag gggcgccatt
     4801 gatcgctgat gtaatcaaag ctttagaggg aacagtcgtt atccacattc ttgaagtagt
     4861 aggtggaggg ttagcggcaa tcggtatcgc gacaactgta tacgttatcg gtcgaaaaga
     4921 ttatctggtc tttttcttcc ttgcttattt tatgagtgtt gttttgaaat ctttagatat
     4981 tacgatggtt acttatgcca tttttgggat tattatcgct ttgatctttg tccagtcgca
     5041 aaaagggaaa caagtacagc ctgccacaag caataacaat tcagcatctt ttgatgacga
     5101 agatgactat gatgacggat tttaatttag gaggaatggg aaatggctga aataatggat
     5161 aatcaaacag gtgctcctga agaaatcacg aaaaaagatg taacaaaagc ctatctgaga
     5221 tggcattttg caaacgaaat tcctcactct tttgaacgtt acttggcgcc atcactacta
     5281 tacggaatga tgccaatctt gaaaaaactt tataaagatg aagactcttt aaaagcagca
     5341 tataaacggc agttactgtt cttcaacact caattaagct ggggtggcgg tgtgattact
     5401 ggcctaatgt cttctatgga acagcaaaga gcagaagaag agtatgaaga aaaagagatc
     5461 ttgatgcagg atgacttgat gtataacacg aaagcgggat tgatgggggc actagcagga
     5521 atcggagatg caatcgattc aggaaccgtc caatatattt ttatcgcaat cgcggtacct
     5581 tgggcacaac agggaagtcc tttaggcgca ctttttcctt ttatctgctt tgggttgtat
     5641 caggttttac taggggtatt tttcgcaaga caagctttca ctatgggccg taatgctaca
     5701 ggcctaaagc aaaacactgg tgttcagaca gcgattgaaa tgctatcagt tttagggttg
     5761 tttatgatgg gggtgcttgc gggaaactat gtaaaagtag aatcttcatt gaaatttgcg
     5821 atatccggac gtgagtttgt tatccaggaa attcttgatc aaatcgtgcc agggatatta
     5881 cctttagctg tcgtaatggg tgtgtacttg ttctatacga aaaaaggatt gaaagtcaca
     5941 caagcattat tatggctgac tggaatatta attgtgctag caggaatcgg tattttataa
     6001 aaagaaggga acgtgaataa gatgggtgta gtcaatttag cgcgtgtgga tgagcgtttg
     6061 atccatgggc aagtaatggt aacgttatct caaaaaagtg gagtgaattc catttttgtt
     6121 gtagacgaag ttgtagcaaa agataaattt atgagagatc tttacaaaaa tgcaggaagc
     6181 cgtacaggac aaaaaacaat tgtcattaca ccagagaaag ctaaattcta ttgggatgaa
     6241 tatcaattta aagaatacaa ttgtattttg atcgcaaaaa cagtccaaac gatttacgac
     6301 ttagtaaaac acggaatccc catggaagaa ttgaatattg gagggattgc tcaaaaaaat
     6361 cctgatacag atcttttagt gacgaaatct gtttatttaa ataaatcaga tgctgaaaaa
     6421 ttgaaagaaa tgaatgaaca atatggcgta aagaatattt attttcaagc cactccatct
     6481 gcacctaaaa caggattaaa agatgtattg aacaagtttg atttgtcata aacaaatgct
     6541 tagaaaaaag ataaagaggg cgaaaaaatg aagaagaacg agctgtttcg tgattgggaa
     6601 tttcgttatc gctatattta ccgtaagcgc cgtacaaaaa agagtaaaca gcgttttctt
     6661 tcagcattag tatcagatat ttacagtatg agaacagatg tgacagtcat tgcttacgat
     6721 acgctagcct accgatcaaa aaatatttat gttggagata tcgaaaaagc agagaaagtt
     6781 atctgtactt attacgatac acccgttcat gcattaggaa gttatttcat gtttgattgg
     6841 aaagaccaaa ggaaaaaaac aatctactct atattattgt ccttcatttt gttgttcagt
     6901 ttaggttggt ggggaatgat gatatataac aaaaatcctc atcacgtatt cgatctgcta
     6961 tccgtataaa caggtatcac tgtactagca tttgggagct actttttctt attgggaaaa
     7021 gcagctcgcg gatggcctag cagacaaact tttattcgaa atacttcatc catattaacg
     7081 atgctagaaa tgatccgtac gattgatgat ccgagtgtag cgtatgcgtt tgttgatgaa
     7141 ggctgttatg gagaaaaggg cttagacagc gtgagatcgg gtatgaaaaa agaggctatt
     7201 cttttttatc tagactctgt tggggcagat acaccgctac agttttcggg aaattatttt
     7261 tcaaataaag aacagtggct gaaacaagtt gataagttga aagaaaaaaa cgtcaattat
     7321 atttttagtg caagaaaaaa gcaagcgcag ttcttctatt tgaccaaaac agatttacga
     7381 gggaaaacat ttaattggca aaatgccaat cagattattg ctctttttcg ataagtaaaa
     7441 agtgcaaaac aaggagtgag aagactaatg ttggaaatca tgatcgttac acatggtaag
     7501 ttgagcgaag gactgaaaga tgcggcggaa gtaattgtcg ggacatcgag ccatattgaa
     7561 acaatgtgct tagagtctgg ggaggacatc caaacattcg gagagaaggt caaagaaaaa
     7621 ttaactatga tcaacacagc agaacccatg ctagtatttg ttgatctaat tagcgcaagt
     7681 ccgtacaatc aagcgctctt ggcagtcaat gaactgactg aagaaaagaa agcagccact
     7741 tatgtcatag ggggcgtgaa tctgccaatg ttgttagaag caatcaatca tcagatgctt
     7801 cacacaccga tagaagaagc ggtggaacaa attgtcaaac aaggaaaaga taatttaact
     7861 gtttggcatg cttcagcgga agaaaaaaaa ttagatgagg acgattttta gaaggaaggc
     7921 tgaaaagatg aagtggggat ttagatggta tggtgaaaaa ggggattcga tcccattaaa
     7981 atatgttcgc caaataccgg gaataaatgg gataattgga actttgctga ctaaaatgcc
     8041 tggagatgtt tgggagattt cagaaattga ggagttgaaa gcatctgttg agagccaagg
     8101 actggaactg cttggaattg aaagtgtagc gatccacgat gcaatcaagg cagggacaga
     8161 tgaacgagat tactatattg ataactatat acgaacaatc aaaaacttgt ctcgatgtgg
     8221 ggttcgattg atttgttata gcttcaaacc aatttttgga tgggcgaaaa ctgatttgtt
     8281 taatgaaaac aacgatggaa gctattctct tttatacgat cagaaaatcg tcgatcagat
     8341 ggagccagga gaaatgtatc aattgataca cagccagtca aagggattca aattgccggg
     8401 ttgggaagaa gaacggttga aaaaattcgc tcagctggaa aaactgtatg aaggaatgac
     8461 cgaagaagaa ttatttgaaa atctgactta ttttttagaa aaagtgattc cagtgtgcga
     8521 aaaagcagat gtaaaaatgg ctatccatcc agatgatccg ccatgggaac tttttggttt
     8581 tcctagagtg acgaaaaatc ttggcgactt gaagaaaatt ctaaatgcag tagattctcc
     8641 atataatgga ctaacgctgt gcacaggatc tttaggggca aatccggaaa acgatttagt
     8701 tgaaatcatc catgaggtag gttcgcgcat ccattttgtc cacttcagaa acgtggagtt
     8761 tttaggagaa agaaagttca aagaaacggc acatccctct acagaaggct cccttgatat
     8821 gtatcatatt atgaaagcgt tggtgcaaga agggttcgac ggtcctatac gccctgatca
     8881 tggtagaacc gtgtggggtg aagtggctat gcctggatat ggcctgtatg atcgtgcaat
     8941 gggactcact tacatgcaag gtctatatga ggcgataagt aaggaaaaac cactgggaga
     9001 gcattatgaa taaaatcaag ctcttcatct ttgatatgga tggcctttta ttagaaactg
     9061 gacgtttagc ttatcgtgct tatgtaaaat cagcacaaaa gtatgattat gaaatgcgta
     9121 aggaagtcta ttatttacta actggccaaa cagaaatggc gataagagaa caaatgggca
     9181 ttttgtacgg agaagatgtt ccatatatca aatggcgaga agcaatcaat caatataaag
     9241 aaaaaatcgt gaaagaagat aagagagtat atacgaaaaa aggagcagaa gaaattttat
     9301 cttttgcaaa agaaagagga atccatacaa tcgtggcttc gtctaataca cgggtaaaag
     9361 tagaaatgta tttaaaaatg gaaaatctat atgccttgtt tgatcaaatc atttcagggg
     9421 atgatgtaaa aaaaggaaag ccggaaccag agatcttttt gaaagcttgt tcaaaaatga
     9481 atattcctcc aagtgaagca cttgtatttg aagactcgat tgcagggatc gaagcagcaa
     9541 ggcgagcggg cattttgtct tttctcatca aagatcattt gatagggttg cccgatcata
     9601 atgggaagca taagttgaaa gtagatgtaa gtttatatga atccaataaa gcagactatg
     9661 aattttatga cctgcatcaa gcaaaacgat ttttagaaag taaagggttg tatctctaat
     9721 aaagaggact attttctagt cctctttatt tattgtcctg cacgactgag ggaacagatg
     9781 aaaaagaagt aattttttat tttgaagtat ctaccttatt tgtagtgaac aatttagaac
     9841 aaacacaaaa cgcatctact gt
//
LOCUS       DAZKSU010000094         9101 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000094 DAZKSU010000000
VERSION     DAZKSU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.095"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..855
                     /locus_tag="ACG8Z3_002451"
                     /pseudo
     CDS             55..855
                     /locus_tag="ACG8Z3_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            1093..1329
                     /locus_tag="ACG8Z3_002452"
     CDS             1093..1329
                     /locus_tag="ACG8Z3_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824713.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1336..3288
                     /locus_tag="ACG8Z3_002453"
     CDS             1336..3288
                     /locus_tag="ACG8Z3_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0824714.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3360..3857)
                     /gene="dfrG"
                     /locus_tag="ACG8Z3_002454"
     CDS             complement(3360..3857)
                     /gene="dfrG"
                     /locus_tag="ACG8Z3_002454"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0824715.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            4132..5259
                     /locus_tag="ACG8Z3_002455"
                     /pseudo
     CDS             4132..5259
                     /locus_tag="ACG8Z3_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            5357..5545
                     /locus_tag="ACG8Z3_002456"
     CDS             5357..5545
                     /locus_tag="ACG8Z3_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0824716.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5603..5956)
                     /locus_tag="ACG8Z3_002457"
     CDS             complement(5603..5956)
                     /locus_tag="ACG8Z3_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824717.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            6487..6909
                     /locus_tag="ACG8Z3_002458"
     CDS             6487..6909
                     /locus_tag="ACG8Z3_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0824718.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6906..7136
                     /locus_tag="ACG8Z3_002459"
     CDS             6906..7136
                     /locus_tag="ACG8Z3_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824719.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7634..7834
                     /locus_tag="ACG8Z3_002460"
     CDS             7634..7834
                     /locus_tag="ACG8Z3_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFQ0824720.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7861..9054
                     /locus_tag="ACG8Z3_002461"
     CDS             7861..9054
                     /locus_tag="ACG8Z3_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0824721.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         3178 a         1468 c         1654 g         2801 t
ORIGIN      
        1 atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa tcacatgaaa
       61 attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt aacagaaagc
      121 ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg tacaacgagg
      181 acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg aataacctct
      241 tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat ggatttctta
      301 gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat ttctgcaaaa
      361 gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat ggggattccc
      421 acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac ggtttatcag
      481 gatattaaag agaaactttc tgccgaaatt gtaatcaaac agaaggtaga actgtatcct
      541 aatatgtgtg tgacgaactt taccgaatct gaacaatggg atacggtaat agagggaaac
      601 gatgaccttt tagagaaata tatgtccggt aaatcattag aagcattgga actcgaacaa
      661 gaggaaagca taagatttca gaattgttct ctgttccctc tttatcatgg aagtgcaaaa
      721 agtaatatag ggattgataa ccttatagaa gttattacta ataaatttta ttcatcaaca
      781 catcgaggtc cgtctgaact ttgcggaaat gttttcaaaa ttgaatatac aaaaaaaaga
      841 caacgtcttg catattaagt agttcagttt tggagtacaa aaagtgaaaa tgtaaaccta
      901 aatgagtatc aaattgcaac tgaatttgat actcgttttt tattgttaaa tcaacgtttg
      961 ttgttaattt acaccacaaa atgtaagtta aaatgtatat aaaaatgcga acgtgaaata
     1021 attattgtag gaatgcaaat tggtgtgtta aaatggttac aatttactaa gcaaaaaggg
     1081 gtgcattttc ctatgcaatt tcttgagcca aaaaataaga acgctaagtc tgtggattgg
     1141 gaaatatcag agcaagtaag ggtgatagtt aagcaatacg ctgaatatgc agagcgtact
     1201 gaaagcgaag cagtggatga attcttattg aacattttag acgataagaa gtttattgaa
     1261 tggattgcca acaagcgaag taataagcgg attgttgaaa aaatgggtat caaagataga
     1321 gtgggttgat agacaatgac caagcttaaa agattagcca cgaaggaaga tgaaattgta
     1381 gatgttaaaa ttccgatttc aaacgaagaa ttaaaagata gggctaaaca atatgattta
     1441 ttaactccaa aacgatttgc aacaaggtat aacaaaatgt tgttcttgcc aacgtctttt
     1501 aaatggaacg gttctgaata tcccattcaa tataattact gcatcaatcc gttttgttgt
     1561 aattttggga aagaacagca caaattcaaa gatgtaaaag ggaaaccaag tcgatataaa
     1621 atgactggta gttccaaaga taaaggtcat aaaggtatgt actgcaatga caatcctatt
     1681 ggcagaggtg tatcgcaaaa ctgtacagta acccctttat ctaattggtc tgttgttgaa
     1741 gaaattaaac gccttataga aatcaatagc atccaagatg ttgagccaga ttatcaattt
     1801 cataaagaag gctgctcgga agaagaatca accccattca atgagccgaa gcaattttac
     1861 aaaaggggga aaagtagggg caaatctcaa cgctatcaat gtaaggcgtg taagaagttc
     1921 actaatgttc taccaaaaag agaagaaacc actacttatc accaacaaaa gaacaccatt
     1981 cttccaatgt tcgctaagat ggttgttgga agggtttcag tcagtcgtac ttgtgatatt
     2041 ttaggaattg gagttggaac ctactatcat aagctggagt ggctgtatag gcgttgtttg
     2101 gagtttttag aacgatatga gacacaaccc cttcaaacaa agaaatttaa cgagatgtgg
     2161 cttaatacgg acaagatgca ctactaccta aataacgtcc gtaagaaagg acaaggttca
     2221 aagaaatata cggggtttga agaccttaat atgcaaacgt atatagttgt atctgctgag
     2281 gtcttatcaa gatatgtgtt ccgttctgat gttgcctatg attggaatat ttcaatggat
     2341 gaattaaatg aagatacaag aaaattcaaa gaagaccatc tcaatacttt tagtcgtaaa
     2401 aatgataggt tagattggtc ttattatcca caagaaccat ccgctaacga tagcgaaaat
     2461 aggaatgctt atcttcacga attagggaaa attactaata ggagtagatt tgtcgatggt
     2521 ttaaatgttg atgctcctta tactacaact gctcactatt ggttaattaa acaaatggtt
     2581 aatgccgacg aatggcgaat gattagtgat gatgacttct ctattagaaa cgccttttat
     2641 agagtattca cgaaagaatt aaggttgagc gatgcacatc attttatttg ccaagttaac
     2701 aagacgaaaa gcagaaaaca atgcttaaaa gaattcggac aagcaaaagc agaactgctc
     2761 gattggggag atatcagagg attcaaaaca aagtttctac ggactttggc atctcattat
     2821 ctaactgaat tactaacaag tcatcaattc cacgaagagg ctattagtaa agatggagaa
     2881 agatatcgca aatatgccga taaccctatc aaacatccat tagccactaa ggataaagga
     2941 ttttattcag ttgattgtag aacggattta tctgctctgg aaccaaacga aatcgcaaag
     3001 atgctattaa atgttaatga ccattcgacc aatagcttta ttcaacagat aagacgttat
     3061 atatcatctc ttgaaagacc attaacaaca gctcgtggtg ataagaagag ctatatttac
     3121 gctaacttca atccgaagta tgctcaattt gcgattacaa ttctaagaac ttactacaac
     3181 ttttgtcgcc cttttaaatc agcggataaa aaggttttga cacctgctca aagattaggg
     3241 attacagata aacaatttga ttggaaagat ataatttatt tcaaatgaca aaaagagctt
     3301 ggagtaatcc caagctcttg tgctttatct ttactgttct tcttcaatta acgcacccgt
     3361 taactcaata agttttttct ttcatataca tgaaaatagt agttatacgg atttttatca
     3421 ttctttatcc ctttttgggc aaatacctca ttccattcct cataattcac ttctggaaaa
     3481 aaagtatctc cttcgaattc atgatgtatt tttgtgatgt acattttctc aacataaggg
     3541 aaaaacaaat tataaatctg ttctcctccg aaaataaaaa tttcttcttc gtttttacat
     3601 aactcaaaaa catcttctat tgaatgaaca atttcacaac cattaaaggt aaacccctta
     3661 tctctcgtca gaataatatt tcttctgtca ggtaaggctc ttccgattga ttcaaggttc
     3721 ttcctaccta atattatcgg atgtcccttt gtagtatttt taacatattc ccagtccttg
     3781 ggaatcctcc aaggaatgtc attctctttg ccaatcactc tattcttatc catcgcagca
     3841 atcaaagaaa ctttcattta taacacagcc tttttaacta atttaccatc taatttctta
     3901 tttctatcaa aaaacctttt ctcattatcc tgcccgttag ttgaataagc aattttttct
     3961 ataagttata ttatcataat accacaaaat cacaaatttt cattcaatta tcattttcaa
     4021 ttgcatttta taaatcattt tgtatgtaat ttggtatgta agttaaaatg aaaaaagtag
     4081 tccattttga agtacatttt gtcattttgt actccaaaac tgaactactt acttgcatat
     4141 atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa
     4201 aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga
     4261 gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt
     4321 ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa
     4381 acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa
     4441 atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata
     4501 ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat
     4561 catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat
     4621 gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta
     4681 tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga
     4741 tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa
     4801 ggattatatg gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat
     4861 agccctgtta gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc
     4921 tttagaaaag ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca
     4981 caggaatatc tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat
     5041 actcaactga aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa
     5101 gattatcgca atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta
     5161 aaaggatatc aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg
     5221 atagataaag taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata
     5281 aatatggttt cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg
     5341 aaaggaggag attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa
     5401 aacaagacta cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa
     5461 atgtaagaac gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc
     5521 agacgccaag acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt
     5581 ttgtttatct gaattttact gactagcctt caatatttct tgcttcattg ataccttttg
     5641 ctagtgcttc cattaaggat agttctttgt ctgtaaagct atccatgtat ttttctacct
     5701 gtaaccttcg agtgctttta atcaaattat ctgtaggtaa gaaaaattca tcaatagata
     5761 catggagtaa tgatacaaga tcatacagca cttgtgtact tgggtgttgt cctttgtttt
     5821 ctatatttgt taaataacgt gggtcaattt caatcattgt tcccacttgt tcacgagtga
     5881 gaccccgttt cattcgagcc tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat
     5941 cttctttttt acgcatacta aatcacctct atacatttta ctgttcctat ataaccataa
     6001 acaggtatag aaaaacatgt tatatagttt atgagttcct atagaacact gtggaattta
     6061 ttaatgcgat gaatccaatt tcaaatagaa aggtcgctga ttttatttac ctatacaact
     6121 ttataaaatt tagtcatact cctagaaagt agataaaatt ggcgacttaa tggagggaaa
     6181 agtcgccatg agaaagataa tctgtcatct agttctctta attaagttct tatcaaaaaa
     6241 acttatccac ctgtactgga tagatatgac catcaatgtg gttttgcaga ttacatagat
     6301 agaaaaatca aagcatatga acagagaaaa caatctgttt atgtgctttt ttggtcactt
     6361 aataaatatc ttttaggatt tctcttcatt tatgcaacaa atcccccgtt cgtgtcgggg
     6421 tttagtatga agggagataa agaagcacat gccactccct ttcacgacag aaagggggtg
     6481 aaaagcatga aaccatcttc ttttcaagaa accatagaaa atcagtttga ctatatctgc
     6541 aaacaagtta ttgaagatga acgaaaagat tacttcaaac atctagacag actatcagca
     6601 aaagaagtct cgttttctga tatagataac taccttgtca actctttttc tactgttgat
     6661 acgtacgtca ctgatttcca actatttaaa ttatttggat taagaatcaa tgttgaaagt
     6721 gatttattaa gtgaagccct gcgtcatctt tcagagaaaa aacggaacat tattttactt
     6781 cattacttta tggatatgag tgatgtggag attgcggagt tactaaattt gaatcgttcg
     6841 actgtttatc gtcatcgaat aagtggacta gccatgatta aagaattcat gaaggagtgt
     6901 aaagaatgaa aacagcttat ccaagggttc ccttccccct cattgtaaag gcgacagatg
     6961 gcgacgtgga agcaattaat cagattgtta aacattatcg agggtatacc tcaaaacgtt
     7021 cattacgtcg catgacagac gaatatggaa attctcatat ggttattgat gaaaccctac
     7081 gtggcagaat ggaaacgaaa ctcatctcta agattttatc gttcgaaatt aaatagtccc
     7141 attttttcgt ggaagcgtgt cgaatcgtat ccacgcttcc cgatttggga ggtttgttat
     7201 tccatcaaag catattgtgt gttcagtagg ttttgatagg ctaacaagcc aaacgttctt
     7261 tgacaaccaa ataaaggcaa tcgaatacgt ttcggtaaga aaagagctga tgaactaaca
     7321 cgccatgacc tacaaaatta gtgagcgatt catgttagta attcgaaagt aatctttagc
     7381 aggattgctg ccatgacttt cttatcgtga taatgatact cccgtatagc catagtccga
     7441 gcgttagaag cgtcacaggc aatgggtacg gctacatgag aaccatgcag aagtgaaatt
     7501 cctgtgagct ttgctagagc tgttcgattg ctaagatatt cttctcttgt cagtgtatta
     7561 ttataaaact atttgatata attataggtg aatattggag ttttcctagc ctataattgg
     7621 gaaaggagta ataatgataa cggtcacaaa aaaggattta gttgagctag gatatggaac
     7681 ttctttttca gcagacatca taaaaaaagc aaaagaattg atgattacaa aagggcatat
     7741 ctactatcaa tctcgaaagc tagatagagt ccctcgtgaa gctgttgaag aaattctagg
     7801 aattcggttt tcaaatgaaa acaggtctga gtagtttttg cacagtaagg agtaaaaact
     7861 atggttaaag acccaataaa aaaagcaaag aatggaacct attattttcg tgcaaatcta
     7921 ggttatgatt ctaaagggaa aaaaattcaa aaatatcgta gtggattcac tactaaaaaa
     7981 gaagcaagag aagaatattc gaaactgcta ttgatgaaac cagaagaatt aagtgagaat
     8041 aaggataaaa tgacgtttaa acattatatc tttgaaattt tccttccatg gtataagaca
     8101 caggtaaagc tcagaacata tgaaaatcgc ttaccaacta ttaagaagca tttctcttat
     8161 tttgataaaa tggctgtttc cgatattgag cctattgatg tgcaaaattg gcaactaaaa
     8221 ttatcaaaga agtgtaagtc ttcttatgta agagctgttc aaggactatt ttcagttgct
     8281 atggatcgag caattgtcct tgggataacc accacaaatc catctaagat tattggcaat
     8341 gtgaaaaagc aaaagtctaa aattgaattt tggacaaaag aagaatttga aaaagtaatc
     8401 tctctaattt acaaagaaga ttactatcaa cactttttat ttatctctct gtggttttta
     8461 tttatgactg gaatgcgaat tggtgaagct actgccattc aatgggaaga tattgatttt
     8521 gactcaggag tactaaccat caacaaaaca ctctattaca agaatcaatc gaattataga
     8581 tttgttgaac caaaaacaaa ggcgagcgtt cgtcatattg tattagataa atgcacatta
     8641 acttatttat ccgaatggaa ggaagtacaa caaaatctaa ttcagacaga ctttgtaatg
     8701 agctataacg gtattcctac ccaaaaacat acactatcat atgctattga acgctatgct
     8761 aagctggctg gtatccatcg aatcagaatc catgggttac ggcattctca tgcttccttg
     8821 ttaatcagta tgggagaaaa tccacttatc attaaagatc gtctaggaca cgaagatatt
     8881 gagacgactt taggaacgta tggacatctt tatcctaaca gtaatttcga ggtagctaac
     8941 aaattaaatg gagccatttc ctttaaagaa gcaaaacaaa attatgatac ttcaccaaag
     9001 aatcaattta ctgtagacta cttgcgaaac aaagaaatga aaaaagtgca ataaaagtgc
     9061 agtaaaaaga taagaagagc cagaaaccct tggtataaga g
//
LOCUS       DAZKSU010000095         8817 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000095 DAZKSU010000000
VERSION     DAZKSU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.096"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(561..1601)
                     /locus_tag="ACG8Z3_002462"
     CDS             complement(561..1601)
                     /locus_tag="ACG8Z3_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0824722.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1950..2276)
                     /locus_tag="ACG8Z3_002463"
     CDS             complement(1950..2276)
                     /locus_tag="ACG8Z3_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824723.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2263..3051)
                     /locus_tag="ACG8Z3_002464"
     CDS             complement(2263..3051)
                     /locus_tag="ACG8Z3_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0824724.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3419..3709)
                     /locus_tag="ACG8Z3_002465"
     CDS             complement(3419..3709)
                     /locus_tag="ACG8Z3_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0824725.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3845..4024)
                     /locus_tag="ACG8Z3_002466"
     CDS             complement(3845..4024)
                     /locus_tag="ACG8Z3_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824726.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4021..4371)
                     /locus_tag="ACG8Z3_002467"
     CDS             complement(4021..4371)
                     /locus_tag="ACG8Z3_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824727.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4364..5689)
                     /locus_tag="ACG8Z3_002468"
     CDS             complement(4364..5689)
                     /locus_tag="ACG8Z3_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0824728.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6139..6753
                     /locus_tag="ACG8Z3_002469"
     CDS             6139..6753
                     /locus_tag="ACG8Z3_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0824729.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6918..7160)
                     /locus_tag="ACG8Z3_002470"
                     /pseudo
     CDS             complement(6918..7160)
                     /locus_tag="ACG8Z3_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7364..8683)
                     /locus_tag="ACG8Z3_002471"
     CDS             complement(7364..8683)
                     /locus_tag="ACG8Z3_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0824730.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT         2680 a         1598 c         1389 g         3150 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 tttgtatttt caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatat cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatatgcaa acactagttc
      361 cctatttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg
      481 taacatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta
      541 tagtgatcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa
      661 ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt
      721 tgataagctt tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc
      781 agttcttcat aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga
      841 atagtttgga cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt
      901 aatacatttt ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta
      961 tctagtaaaa cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg
     1021 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
     1081 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct
     1141 cgcggttttg ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct
     1201 tccatgattt taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta
     1261 ggcatggatt caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga
     1321 cctgcttttt ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat
     1381 ttcattaatt cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta
     1441 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
     1501 gatagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
     1561 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
     1621 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
     1681 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
     1741 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct
     1801 catagtaact atatcacgat attacaataa tattatatca cgatatcgta atattacaat
     1861 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1921 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1981 tatccttcaa tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct
     2041 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     2101 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     2161 cttatatttt tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta
     2221 tttttatttc ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa
     2281 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     2341 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     2401 cctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     2461 agacgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg
     2521 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     2581 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     2641 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     2701 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     2761 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2821 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2881 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2941 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     3001 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     3061 ccccattcat cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt
     3121 atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg
     3181 atataaagat tattcatatg ttattcgaaa ttaaaataat cactttttat tactttaaaa
     3241 ttttttatta atgataagaa atatgttttg cgagcagcaa ctagaataat ttaaacatta
     3301 ctaaactgtc tatattgtta ctaataattc ccttcttata gtgcattttc ttagaaaaaa
     3361 acaatattac gatatcatga tattaagata tcgtaatatt atacaattgt gattgatatt
     3421 atatatgttt ttttattttt ttatcatgtt taatcttgaa ggaatcatat tctttttcta
     3481 aagacaggag cttttcataa acatcagaca tgtttaaact agaggccagg cttaaatcat
     3541 aaacgaatga agcaggtaat gattttattc tacgttgtcg ggttatccag cttgcaaccg
     3601 ttccttgtgg gtaggcatat aactcacaaa attgttctac agtgattcct aaattcttga
     3661 taatgtaaac attgatcggg tgcgagtaaa caaatgtatt gatagacatg gtgcgtaagc
     3721 ctactttctt ttattattgc gtttgcaata atagtttaac acacgcatta ttctagtatc
     3781 tactcctatt tgtaaaaaca aaaaaggatt catagagata actctataga atccgtaaaa
     3841 taccttatat atataacggg aagatataaa acctaaaatt atgataacct gctgcagctt
     3901 ctacatagaa agagaagcgc tcaacaccaa cctcatttat caaacgttgg ccataacccc
     3961 aaatctcttc tgatagctga tcaaaggtta tatactttag ctcatattgc tggataatgt
     4021 tcattctaat cgactccatt tattatcctg gtaaaattcg atatgacgta tttcgtcgta
     4081 gtgaattttg tcttttccaa cgtagatccc cagctcatca ctacctaaga tcttgccaat
     4141 gatatcttct ggatagtatc cttcagcgtt tcgttcttct ttttgaatag atactgatct
     4201 attttttatg actgcagcgt tcaatacttc cgcaatctct tcagtcgtca tttgttcttt
     4261 tgctaaattt tgactggttc gttgttcatt gtcggcatca atttcttcag tatgatcaga
     4321 taaataaaac cctgcccact ttaaacgtcc acgatcattg taatcaatca tttttaattc
     4381 ccccatttcc gcccgcatga ccaccaacaa gtgtgcttct agtaatgctt ctagcgcctt
     4441 ctaacattga agaagcatgt acaatcgacg taaaaccata tttttgccga atcttgtcaa
     4501 taattttatc taaattttct tcgtcaattt gcttttgtgg atcactaaat aaatccagct
     4561 gaagcgaatc ggtgtacaca agcttgctat aggttattcc aacatgcctt acttcttgtc
     4621 cttcataata cttatcaaac aagaaaagac aataattctg caattccttt gtattatctg
     4681 tgatagggat cttcatctgg tgtgaaaacc ctggtctcgt ttctaagatc gacgtgccaa
     4741 tgtgtaaatg gacacaacct gttttacagt tatgctgcct aatgcgtatc gctacttgtt
     4801 ccgccatttc ttttaccacc agctcgattt catttcttct agtataatct cgtggtaaca
     4861 cttgtgaatt tccatatgat ttttcctttg tcggttctgg tgggattgct atatctgttc
     4921 gatcgatccc attagcatgg aagtacagct gtaagccaat cacacctagt ctattcttaa
     4981 tcgtataagg attccagtta gcaagatctc taatgctcat aatccccatc tgattgagtc
     5041 tttttttcat tcgagagcca attccccaaa agtccgtcat ttcaggaata ttccatactt
     5101 tttcaggaac gttttcgtac gtccattccg caacaaatcc ttcattatgc tttgcttcgt
     5161 tgtctaaggc tagtttagcc aaaaggggat tatctccgac acctactgta gcaatcaatc
     5221 ctagctcttc tttaatattc tcctggatca tttgagccag cttctttctc cgttgacttc
     5281 gtgttccttc agtcgtaaaa agattcagtg atcgggtcac ttttaagatt gattcatcga
     5341 tcgagtaaat cagtagatct tcatcagcca catatcttct gaaaatatta tttacctgca
     5401 tatttcgctt gatatatagg ttcatacgtg gtggaacaac gtgtagtgtt ttaggaaatg
     5461 gttgtggtaa gtcacgtggt ctactcacat ttgtaatacc ataccgcttt tttgcttcag
     5521 gagaagaagc taatatcaat cctgaaccag tattatcagc tcgactcata acaacaagtt
     5581 ctgttgttaa tggatctaaa tttctttcta tacattcgac acttgcgtaa aagcttttca
     5641 tatcgattaa aaagtaatca tttactggct cttttgaata atccatcata aataacactc
     5701 cattctttca cattatacaa acatatgttc gatattgtaa agaagaaata gctaaataca
     5761 taaaaaaaga agcataggta tgctatcacc ctgcttcttc ttttgatttg tatgatcaat
     5821 ttattctatc actttactaa ttaatttgaa aatctaataa aaactctcac tctaaaagag
     5881 tgagagactg caaattagag tacggacttt tttggagttc cgttctttaa tattaacaaa
     5941 tgaataaaat ccttacaaca tttgagaata agttatcagt agacacttac aaaaaattct
     6001 catactattt cctccatcat tagtttttgt ctatataagg tttaccctaa atagacactg
     6061 gtataacatt gaaaaatatg gcttcctcgt gtatgattag ggtgtatttt aaatagttgc
     6121 agaaagtagg taaaagtttt gagagttgca tacatacgtg tctcatcgat cgatcaaaat
     6181 gaacaacgcc agattgaaga aatgaaaaaa ttcggtgcag agagaatttt catcgagaaa
     6241 caatctggcg caacgattac tcatcgacct gttttccaag aagcattaga ttacgtccga
     6301 gatcaagata tttttattgt ggaagctatt gatcgacttg gaagaaatta tgacgagatc
     6361 atcgcttcag tcaattactt gaagaagaaa aatgtgaagc tgatcattac tagtttaccg
     6421 atcatggctg aagcaattgg caatcctctc ctggataaat tcattaagga tctgatcatc
     6481 caaattttgg ccatgatcgc tgaacaagaa cggactgaat cgaaacgtcg ccaggcacaa
     6541 ggaattaaga tcgcaaaagc aaacggcgtc tacaaagggc ggcccaaatt atacagtgca
     6601 gacgcaaaag atccccaacg tcgccttgtt tacaaaagca tcgttgaaga tcttaaaaat
     6661 ggtgttgcga ttgctaagat tgctaaagac tacaatgtta cgagacaaac cgtgtataga
     6721 ataaaaaaag acagcatggt caatgacaaa tagttgatca tgctgctttt attatcttct
     6781 catctttaat ttcctacata taattactat ctataaaatt aagtcttact cagaacagat
     6841 aaagcataaa attccaacaa accatttttt acttaactaa ataaagagtc tgttaatata
     6901 atcttcactt atttacgtta atcccctact gtatttgttg gttccttatc tatattttct
     6961 acaaaatcct tattatcttg tgatttagct tcgtataatt ctttgtatgt cgacacattg
     7021 gttatactaa ttcctatagc tagttcttga gactctactt ttctctctag ctcctttgac
     7081 aaagtgattt gagatttatt tggttccgtt ggagtcaatt ctatagattc ttcataaaaa
     7141 tctctataca taaagctatc cctgaataat tcatagcttt aattctttgg cacattttat
     7201 tgaaatatgt atcactaata tccctatcaa ctgattttga ctaaaaagtt acttcaaaca
     7261 ttcactgttc aaaaatattg aggaataaat tttgggtgta aggcattttg agcatacttc
     7321 tccctggata acttcccagt aaaaaaatcg ttagattgtt ggattagatc cattgactgg
     7381 ctcgcctcag gcgagcaaag accctgtaga attcattctg atacacatga taactagata
     7441 atttgagata gactcgtcta cccgtttgga ctaatttccc agcaactttc agaaacttca
     7501 atcgaataga atgaatggtc attccctttt ggacttcttc aaagccaatc gttcttaaga
     7561 agttgactaa gttgtaagct atcaagctga gcatcattcg gacatgattc tccaaaaagc
     7621 gaggactgtc tgttttgtca aagtaaaagc cagctttcgc ttctttaatg aagttctcca
     7681 ttgtgccacg tttggcatag atagaaaaga tggtatcagg agaaacattt tctgatagat
     7741 tcgtcacgat aaattcatgt cgaaagagta gttcgcccgc ttcacgtgtt gagcgtatac
     7801 acacgcgacg actttgtgac caggattgtg cttgataagt ggtcgagaag tactgaactt
     7861 ctcgttcttc ccacttttga ttatcgccat aaagtactga tttctcagct atttgaccta
     7921 gtctacgatt attcttcagt cgaataacat aatgactctt ttttgattca cacgaatcat
     7981 acacaccagg tgtagcgaac ccgctgtctc cacgaaccaa gatgtcagtg tttggtagag
     8041 agtgattata gtgctctaat aaaggtgtga gaaactcctt tacgcctttt gatgtatatt
     8101 gatttcctga acgtagttca gcttttaaga agtcaccagt caatccgtca aaagctacca
     8161 atggatggta accataggtt tggtagtgtg tattataatt cgtttgttct tggtgaccaa
     8221 atgtatctga atgggtcgaa tctaaatcaa tgattaattc cgtatcatta cggatgaggc
     8281 gtgctttatc gataagttct tggttcaaag cttgaagttc atggatattc tcctcagata
     8341 gtcgatctaa aaatcgggaa agtgaagatt gagaagcgag ttctttccta tctaaaacag
     8401 ctttaaacac aggatcttgt ctcaggagat tagctgcaga atcagctgag taaccagcaa
     8461 ttaattgcat aatgaactgc tcaagtatgg ataagttgtc atgagtaaaa tacgctcgtt
     8521 cgtcttcaat gtgaatgtgt tgcttagcca gttcagaaaa accaaagatg ttcatcagct
     8581 ctttcactag gacgagaccc gaatcactcg ataattgacc acctgtatga gaaatagtga
     8641 tatttgaatt gaattttact tggtttttgt gtaagctagt catgaaaaga actcctttct
     8701 tttggttggt ttagtcactt taaccatagc agaaaggggt tcttttttca tcacttaacg
     8761 ggtgaagatg aaaaagtaat tgtaagctgc cgtgaggcag tttcacatgg ttttaat
//
LOCUS       DAZKSU010000096         8735 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000096 DAZKSU010000000
VERSION     DAZKSU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.097"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(474..1061)
                     /locus_tag="ACG8Z3_002472"
     CDS             complement(474..1061)
                     /locus_tag="ACG8Z3_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824731.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            1236..2558
                     /locus_tag="ACG8Z3_002473"
     CDS             1236..2558
                     /locus_tag="ACG8Z3_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0824732.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2713..3489
                     /locus_tag="ACG8Z3_002474"
     CDS             2713..3489
                     /locus_tag="ACG8Z3_002474"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0824733.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3482..4051
                     /gene="rnmV"
                     /locus_tag="ACG8Z3_002475"
     CDS             3482..4051
                     /gene="rnmV"
                     /locus_tag="ACG8Z3_002475"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0824734.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            4044..4928
                     /gene="rsmA"
                     /locus_tag="ACG8Z3_002476"
     CDS             4044..4928
                     /gene="rsmA"
                     /locus_tag="ACG8Z3_002476"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0824735.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5379..6008
                     /locus_tag="ACG8Z3_002477"
     CDS             5379..6008
                     /locus_tag="ACG8Z3_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824736.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(6132..7241)
                     /locus_tag="ACG8Z3_002478"
     CDS             complement(6132..7241)
                     /locus_tag="ACG8Z3_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824737.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7450..7872
                     /gene="mgsA"
                     /locus_tag="ACG8Z3_002479"
     CDS             7450..7872
                     /gene="mgsA"
                     /locus_tag="ACG8Z3_002479"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0824738.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      8059..8157
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            8195..8731
                     /locus_tag="ACG8Z3_002480"
     CDS             8195..8731
                     /locus_tag="ACG8Z3_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0824739.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT         2766 a         1543 c         1790 g         2636 t
ORIGIN      
        1 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
       61 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
      121 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
      181 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
      241 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
      301 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
      361 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
      421 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
      481 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
      541 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
      601 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
      661 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
      721 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
      781 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
      841 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
      901 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
      961 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
     1021 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
     1081 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
     1141 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
     1201 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
     1261 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
     1321 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
     1381 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
     1441 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
     1501 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
     1561 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
     1621 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
     1681 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
     1741 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
     1801 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
     1861 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
     1921 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
     1981 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
     2041 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
     2101 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
     2161 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
     2221 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
     2281 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
     2341 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
     2401 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
     2461 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
     2521 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
     2581 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
     2641 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
     2701 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
     2761 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
     2821 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
     2881 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
     2941 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
     3001 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
     3061 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
     3121 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
     3181 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
     3241 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
     3301 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
     3361 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
     3421 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
     3481 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
     3541 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
     3601 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
     3661 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
     3721 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
     3781 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
     3841 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
     3901 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
     3961 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
     4021 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
     4081 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
     4141 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
     4201 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
     4261 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
     4321 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
     4381 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
     4441 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
     4501 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
     4561 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
     4621 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
     4681 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
     4741 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
     4801 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
     4861 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
     4921 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
     4981 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag
     5041 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
     5101 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
     5161 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
     5221 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
     5281 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
     5341 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
     5401 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
     5461 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
     5521 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
     5581 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
     5641 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
     5701 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
     5761 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
     5821 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
     5881 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
     5941 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
     6001 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
     6061 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
     6121 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
     6181 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
     6241 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
     6301 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
     6361 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
     6421 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
     6481 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
     6541 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
     6601 ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
     6661 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
     6721 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
     6781 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
     6841 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
     6901 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
     6961 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
     7021 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
     7081 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
     7141 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
     7201 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct
     7261 tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat
     7321 gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat
     7381 ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga
     7441 gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa
     7501 ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact
     7561 gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta
     7621 ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt
     7681 cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta
     7741 tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga
     7801 ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca
     7861 ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc
     7921 tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa
     7981 caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg
     8041 aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga
     8101 tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga
     8161 tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg
     8221 tatgattgct attatgggct tattgattgc attgatggtc actttgtccc gactggtagc
     8281 aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat
     8341 tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc
     8401 cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc
     8461 aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac
     8521 gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta
     8581 ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg
     8641 gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat
     8701 tttaagaagt ttagcgatcc acgctaaata aaaaa
//
LOCUS       DAZKSU010000097         8453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000097 DAZKSU010000000
VERSION     DAZKSU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.098"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1329)
                     /locus_tag="ACG8Z3_002481"
     CDS             complement(<1..1329)
                     /locus_tag="ACG8Z3_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0824740.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIKPVVLVNFNILKNISKFL"
     gene            complement(1470..2072)
                     /locus_tag="ACG8Z3_002482"
     CDS             complement(1470..2072)
                     /locus_tag="ACG8Z3_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0824741.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2317..2523
                     /locus_tag="ACG8Z3_002483"
     CDS             2317..2523
                     /locus_tag="ACG8Z3_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824742.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2835..3554
                     /locus_tag="ACG8Z3_002484"
     CDS             2835..3554
                     /locus_tag="ACG8Z3_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0824743.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3635..4318)
                     /locus_tag="ACG8Z3_002485"
     CDS             complement(3635..4318)
                     /locus_tag="ACG8Z3_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0824744.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4536..6722
                     /locus_tag="ACG8Z3_002486"
     CDS             4536..6722
                     /locus_tag="ACG8Z3_002486"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0824745.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7068..7478
                     /locus_tag="ACG8Z3_002487"
     CDS             7068..7478
                     /locus_tag="ACG8Z3_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0824746.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            7492..7848
                     /locus_tag="ACG8Z3_002488"
     CDS             7492..7848
                     /locus_tag="ACG8Z3_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0824747.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3106 a         1382 c         1583 g         2382 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gttttatttg
       61 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
      121 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
      181 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
      241 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
      301 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
      361 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
      421 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
      481 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
      541 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
      601 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
      661 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
      721 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
      781 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
      841 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
      901 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
      961 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
     1021 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
     1081 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
     1141 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
     1201 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
     1261 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
     1321 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
     1381 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
     1441 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
     1501 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
     1561 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
     1621 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
     1681 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
     1741 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
     1801 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
     1861 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
     1921 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
     1981 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
     2041 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
     2101 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
     2161 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
     2221 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
     2281 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
     2341 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
     2401 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
     2461 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
     2521 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
     2581 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
     2641 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
     2701 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
     2761 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
     2821 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
     2881 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
     2941 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
     3001 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
     3061 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
     3121 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
     3181 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
     3241 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
     3301 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
     3361 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
     3421 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
     3481 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
     3541 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
     3601 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
     3661 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
     3721 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
     3781 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
     3841 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
     3901 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
     3961 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
     4021 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
     4081 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
     4141 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
     4201 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
     4261 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
     4321 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
     4381 aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt
     4441 ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaacaa
     4501 attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa attctccgat
     4561 tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa gtctttcggc
     4621 tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc ggacttattg
     4681 aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa atgatggaga
     4741 aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat gatatgaaga
     4801 aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag
     4861 ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg atcactttta
     4921 caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag agaaaaaccg
     4981 ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa atttgtcgaa
     5041 aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat actgcacaaa
     5101 aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata tttgatcaat
     5161 atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac ctttcagtag
     5221 aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat gcacgacagc
     5281 cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa agaagccagc
     5341 aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag aatgaggaga
     5401 tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt caagatacta
     5461 atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc aagttgattg
     5521 ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca gcatacatca
     5581 agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat tatcgttctc
     5641 gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat gatcggatac
     5701 caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat ttgttttctg
     5761 atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt caaaaaaatg
     5821 ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag cagcttgtcc
     5881 aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca tatgaaggta
     5941 tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg caaacagcac
     6001 tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg atggcagact
     6061 gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa aatatagccg
     6121 ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca gaaatccgag
     6181 agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg cttgttcaaa
     6241 gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa gaatggatac
     6301 gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg caagaaaccc
     6361 aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga aaacttgatg
     6421 cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat gtctttttga
     6481 ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa gtaatcgact
     6541 ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga ctattttatg
     6601 ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa
     6661 gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt
     6721 gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg aatgtacgaa
     6781 aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta tttctggaac
     6841 acagtttatt cggaaaaggg aggtgtgaca agaattttgt cacacctccc ttatttattt
     6901 tgaaaatgag aatactatta tcaaaataga gaatatatag aaaaattctc attattcaaa
     6961 cgtaaataat cagttatttt gatttttttt atattttatt agctaataat tggtatttta
     7021 aaagaaatta ctgtagaatt ggattataat taaaaaaggg tgagaaaatg ataaaagtat
     7081 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
     7141 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
     7201 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
     7261 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
     7321 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
     7381 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
     7441 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
     7501 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
     7561 catcaattgc cttatcaatt tgttgactta gaagaacgcg atctaagtac agaagaagca
     7621 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
     7681 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
     7741 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
     7801 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
     7861 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
     7921 gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg
     7981 tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg tgtaaattta
     8041 catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta ttttaaaaaa
     8101 catataaaat agttgttgac atgttagaaa tgacatgata agataactga gttgttaaaa
     8161 aagaaccact tgtgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
     8221 aacaaatgat aagttatgat aaatgagttg ctgtaagatt tcgaaaaacg aaatcaaaaa
     8281 aacttttaaa aaagagttga caattacttg tctctctgat atgatataaa agttgctacg
     8341 gcagccgaaa acgaatcggg gaacactcct gatgaagtag acctttgaaa actgaacaaa
     8401 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa aca
//
LOCUS       DAZKSU010000098         8423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000098 DAZKSU010000000
VERSION     DAZKSU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.099"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..505)
                     /locus_tag="ACG8Z3_002489"
     CDS             complement(<1..505)
                     /locus_tag="ACG8Z3_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824748.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILIS"
     gene            471..2107
                     /locus_tag="ACG8Z3_002490"
                     /pseudo
     CDS             471..2107
                     /locus_tag="ACG8Z3_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2252..2992)
                     /gene="truA"
                     /locus_tag="ACG8Z3_002491"
     CDS             complement(2252..2992)
                     /gene="truA"
                     /locus_tag="ACG8Z3_002491"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0824749.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3183..3818)
                     /locus_tag="ACG8Z3_002492"
     CDS             complement(3183..3818)
                     /locus_tag="ACG8Z3_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0824750.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3965..5185)
                     /gene="sstT"
                     /locus_tag="ACG8Z3_002493"
     CDS             complement(3965..5185)
                     /gene="sstT"
                     /locus_tag="ACG8Z3_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0824751.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5753..>8423
                     /locus_tag="ACG8Z3_002494"
     CDS             5753..>8423
                     /locus_tag="ACG8Z3_002494"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0824752.1"
                     /translation="MNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFICY
                     LLAVSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLFVHKSYFLVAAF
                     FYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFW
                     YALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVSPVLLIVGGLIL
                     LLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLF
                     KVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQ
                     TRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGG
                     TANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRR
                     KQVFDKFGFDSFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQL
                     STMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKID
                     KPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNF
                     GAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEK
                     NLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1548 c         1292 g         2798 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggttttctt ctttatttat
     1501 agcctatacg atgagtatag tgatctatta tattttttct actaaaaata attcatggaa
     1561 aataatggtc tttacgtttc ttgcactaat attgcatctc tcaggagtaa gcaattctat
     1621 ccatgctaac aaagattcat taacacttat agcacctgaa gacgctgttg ctatagctga
     1681 aaattacaat catactgatt atgcaaacaa agagtcaatc tatcatcctg atatgattaa
     1741 taatgatcaa tatttactag gtaaccagga aataaatcca acaactcatt ttatgagtaa
     1801 caaacttaca attgaattag ataattcgaa taataaaaat actgtcttga cgacacctat
     1861 ttatcgttat aaagggcaag tggcttctat taatggcaaa ctagtacaaa caaaattatc
     1921 taagtttggc acaactgaat taactattcc tccaggtata aataaagtcg ttattactta
     1981 tcagtacaca aaactagcaa ttgcttcaag atatttatct atcgttactt tgattttatt
     2041 tttactttat cgatttactt tttctaaaca aaaacaaccg agaagagaag tacaacattc
     2101 tcactagatt tttatttaag aaagactttt tagctgtaca cataaacagg ctaaaaagtc
     2161 tttttttata tttctttaat ttgcttcata cattatttaa catcgatatt tatactgtga
     2221 aacatgcgtt tcatccatta tcaaagtatt tttatcgata ataaacttca tccaaaaaca
     2281 gaccttgtgc agggacggtt tctcctgcat gttgccgcac tttgctttca aatatttcat
     2341 cgattgtttc tatcggcatg gcacctgtgc cgatttctag taatgtcccc atgataatcc
     2401 gcaccatttt atataagaat ccgtctccga caaaagtaaa atgaagcatt tctccctttc
     2461 gttcgataga taattcatca atcgtacgca ctgtggattt ttttgatttt ttcaatgcag
     2521 aaaaaccaat gaaatcatgt gtacccacta atttttcgca agctgcattc agcttgtcca
     2581 tatccaactc ttgagggaaa taaaagctgt ggtttcgctc aaatgctgaa ggaatcacgt
     2641 tgttccaaac ataatagctg tattttttgc ccaccgcatt atatcttgaa tggaagcgct
     2701 caggcatttc ttcgattctt ttgatgacaa tatcccgcgg aaggtaacga ttcataaagt
     2761 cgatcatttc tgccagtcca atttctgtgt ttgttttaaa atttgctact tgtcctcgcg
     2821 catgggtgcc agcatctgtt cttcctgatc caataatttc tatctcttca tttgtcatct
     2881 gatgcaatac tttttcgatt tttccttgaa tggttttatc gtcatctcca agtctttgcc
     2941 atcctgaata tctttttccg tcatattcga ttgtcaactt gatgtttctc atattttagt
     3001 ggctcctctg tcttccattt ggtcttctct ctaccttttt aagattgtag cacaaaatat
     3061 agctcctttc ttctctaaaa cagaagctat acaagaaaac tgtggaaata tatatttcag
     3121 aaaaacaaaa aacagccacc ggatgaagta tccgctgact gttcaatttg acggctcaag
     3181 ttttactgtt tgagatgcaa gatactcaac caggcaatgg gcacataatt aaagaaaaaa
     3241 ctgattcttt ggcaaaaacc tattttattc agtaatggcc aagaatttat agaaggaatt
     3301 cttgccagtc cataaatcgc cgcaaaaagc aaactgatca cagacagtcc aaggtataag
     3361 tggcttacgt ccatttgccc attttttcga tagatcaaga aaagaagaac tggaaacaat
     3421 aaaaaaccac tgtaacctaa acctgatccg atattatgga cccatgtcga gacattccac
     3481 gaagattcat gggtattgat actgaacaaa cctgtaaaga tacaatcacc tataccatag
     3541 aacgcgatgg ctgctgtaac tagggtagct aacacacgag atgtacttac aaatgcagca
     3601 tacaatgcag gcaatgacag gatgaaaaag atccctgaaa ccacagacca aataagaaac
     3661 gcttttctta caggactgga aacctctcca aatacactga tcacgctctt catttgatct
     3721 aattcaggat aatacaatcc taatacgtac ggagtcaaaa aatcactcag cacgcctaat
     3781 aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga tacgcctcca ataacaatta
     3841 cctatgcttt taattgttat tataagcgac tttatcttga tttaacagaa acctctattt
     3901 acacgaacat ctatataact cattaaaaac tcgaatcatt ttaaaaatac taaatatcta
     3961 acgttcaacg ttcacttaat ccatctaaac aatctttcaa tttgattttt tccccacttc
     4021 ttttacgcgc gcctagctct cctattgcag taaacaacag atcacttgac gaatttaagg
     4081 cagtttccat tgaatcctgg ataacaccga taataaatcc aataccgacg atttgcatcg
     4141 cgacatcatt agagatacca aacaaactac atgctaatgg aatcaataac agtgaaccgc
     4201 ccgcaacacc tgaagctcca caagcagcta aagcagacaa aatacataaa aggactttca
     4261 aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt caacgtcatc aaggaaatgg
     4321 taatcgcggc accgcccata ttgatcgtag cacctaacgg gatcgatatg gaataagact
     4381 cttttgtcaa tttcaatctt tcagccaaca tcatgtttat cgggatattt gcggcagaac
     4441 ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca aaagaaaatc aatggatagg
     4501 gattttcttt aataaacaaa aacgtgagga atgggtacac aactaatgcg acaaacagca
     4561 tcgtaccgat caaaacaagt aaaagctgca gatactctgc caatccagct attccagtcg
     4621 tagcaattga ttgataaacc aatcctaaaa tccctatcgg agcaaatgca ataatcactt
     4681 gtacgacttg tgacaacgct atagatatct gatccaccaa ctcttttgtc gattctgaac
     4741 gtgctcgaaa agctagtccc aacaatacag cccagaataa gacgccaagg taattcgcat
     4801 caataagaga ttgaacagga ttttgtgtga cattcgtcaa catagtagat aaaacacttt
     4861 ccaaactttg cggtgctttt tcagatactg tttcttgtaa agtaatagga acagtaaaaa
     4921 ttgaacttac aataactgct acaagtgcag aaagcaatgt tcccactaaa tataagatca
     4981 aaataggctt cacgaatgtc ttattcccaa ttttatgctt ggcaattgac gccatcgtca
     5041 agaaaaaaac cagaataggt gcgattgcct tcaagcaact gacaaacaac gttcccaata
     5101 ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa tattccgact aaaataccaa
     5161 caacaatacg ttgaactaac gacatatcca aaaacttctc taacaaatga ccaccctttc
     5221 acaattttaa caatcattgt atcattttat gcaaagaaac taaaggtata attttctaaa
     5281 aattctaaca atacaaacaa ccatttcttg ctaattttgt actttttctg tctacacgtt
     5341 gttttttgag atattctaag ttaggatgtt attaatgggc aacttatcag gaaacgtttt
     5401 cttatgtttt tgtccaattt ttttgtgagg tgtttattct tgaaaaaaat aaagaaggag
     5461 agttatggct tcctgacaat ctttgtcgct tgtataatga tggttttaca gcgtatattg
     5521 atttcagcag tcactattcc tacatatgca acaaaccctg gtcgattcta catctataca
     5581 tttttacaaa cttccgttgt tattggtgca aatttgatcc cactttacat gggttaccaa
     5641 tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa gtcgttttgc ctttatattc
     5701 ctaatagcca cactagtttg caatatcttt tttttacgta caagctggca gcttgaatat
     5761 tcgtgattat tggcttatcc taacacctat cagtcagaat tactttactt atgctgtttc
     5821 ttgtactttg cttttttgta gtattcctta tacagttgga tttttaaata aattatcgaa
     5881 aacaacaatt aaatatctac tattattttt aacaattgga ttagttggct gctctactct
     5941 atttaataaa gacccgtttg cattacagaa tggacagagt attttatgga ttttttattt
     6001 atttttaatg ggttatggtt tgaaagaatg gaactggaaa gaaaaggtcc gctataagct
     6061 tcctcaagta tttatcagct tgattgtttt attcggtttg attattttga tgacacaaat
     6121 ttcactcatc atcagaggaa atacagatac agcgctgagg ttttctaaac catattctct
     6181 cttcgcaatg tattatactt tctcttcgtt tttattttta gaaatgctat ctgataaatt
     6241 aggtataaaa ggaagaggaa attttcttgc tgtatttttg attgtcactc aagttatatg
     6301 taatttccct ttagtttctt atcgcgtatc tacatttatg aaacgtgaat ttccaaactc
     6361 agggaaggca tggctgataa atatcgggca gttttttatt tgctatttat tagcagtgtc
     6421 cctcctagtt atctttttat tttatataca aaaaatccgt ttcattcatc gtttcatcga
     6481 atacttcgct tttgattcga ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg
     6541 gctttttgtt cataaaagtt attttttagt tgcagcattt ttttactgct ttaccttcgt
     6601 gcagattttc gttttagaac caaaagaagg ttggaaagaa actatccagg ttgccttaaa
     6661 aatatttact cagcgacaag cacccatgat cctgacaatc atcattatta tgggattttt
     6721 cttcttacta ttgttgattt ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat
     6781 taacttactg ctcactattt cgacagtaat caaaatggaa atgcgggaag agcctgtctt
     6841 tccttcagat ttaaaaatac tgaccggatt gtcagaacta ttatcgatgg ttagcccagt
     6901 cttattaatc gtgggcggat tgatccttct cctattattg attactagta ttattgtaca
     6961 acgtagacta caaaaacagt attccttgaa gattcattgg aaacgtcgat ttatcagtat
     7021 cgctgtatta ttaggatgtt tttcgggtgt ctttttcatt aatcacaaaa attcgccttc
     7081 tttcttgctg tttaatttat ttaaagtaaa caagactttc tttaaccagc aaggcgccgt
     7141 aaaagataac ggaccagtca ttcaattcct aaacaatatc gacattaaaa tcatggaaga
     7201 acctgctggc tattcgaaag aaaaaattaa tggaataatg aaaaagtaca acacaaaggc
     7261 agatgaaatc aaccaaacaa gaagtgactg gttaaacaat cagacgatcg ttctcaattt
     7321 aagtgaaagc ttcagtgatc ctgcacgtgt ccctaatctg acagtaccta cagaccctat
     7381 tccttacatc aaaagcatta taacagaagg aacaggagga ttgatgttat ccgtcggtta
     7441 tggtggcgga acagctaaca tggaatggga aggactgaca ggcttgagta tcagtaacct
     7501 gtctgcttcg ttagttacac cttataccca gctagtggag cgtcagaaaa taagtccaaa
     7561 tattaccgat ctattcgatg aaaaaatagc catccatcct tttacagctg ctctttatcg
     7621 cagaaaacaa gtgtttgata aatttggttt tgacagcttc tattatttag acagtccgga
     7681 taaattaact tatacggata aaatagaaca aagcccacgc atatcggatg attcagcata
     7741 caaagaaacc ttgaaagctt tacaaagcaa tacagaaacg tcacaattca tccaattatc
     7801 cactatgcaa aaccatatgc cttatgatga ttactatagt gaattgaact acactgctga
     7861 aggaactgct gtcattccta atagaagaaa agaattacaa acttatatgc aaggccttca
     7921 ctataccgat acagcagtaa aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat
     7981 tacctttgtt ttttatggtg accatttacc cgcaatctat agtgggaatg attcgaaaaa
     8041 atatggatta gaacagcacc agacagatta tttcatttat aacaacaagt atagtcgtga
     8101 acaagcagct acgttaaata aagaagttgt tgctcctaat aactttggtg ctatggctct
     8161 tgagcatgcg aatatcaaag taactcctta ctatgcgttg ttgacagaag tatctaatca
     8221 tctacctgca ttaacaattg atcctacaga aagcttgtct aatcgtttta atggaaaaca
     8281 ggtctttgtt acagaggata gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa
     8341 agaaattcta gaagattatc gtctgataca atatgatctt gttgcaggta accagtacag
     8401 tgcaacatgg gcgacacaaa aaa
//
LOCUS       DAZKSU010000099         8228 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000099 DAZKSU010000000
VERSION     DAZKSU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.100"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACG8Z3_002495"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACG8Z3_002495"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0824753.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8Z3_002496"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8Z3_002496"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0824754.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACG8Z3_002497"
     CDS             2591..3376
                     /locus_tag="ACG8Z3_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0824755.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACG8Z3_002498"
     CDS             3389..4240
                     /locus_tag="ACG8Z3_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0824756.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACG8Z3_002499"
     CDS             complement(4364..4816)
                     /locus_tag="ACG8Z3_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824757.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACG8Z3_002500"
     CDS             complement(5281..5649)
                     /locus_tag="ACG8Z3_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0824758.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="ACG8Z3_002501"
     CDS             5799..6281
                     /locus_tag="ACG8Z3_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0824759.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="ACG8Z3_002502"
     CDS             6318..7328
                     /locus_tag="ACG8Z3_002502"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0824760.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="ACG8Z3_002503"
     CDS             complement(7443..8156)
                     /locus_tag="ACG8Z3_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824761.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DAZKSU010000100         7699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000100 DAZKSU010000000
VERSION     DAZKSU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.103"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            544..1107
                     /locus_tag="ACG8Z3_002504"
     CDS             544..1107
                     /locus_tag="ACG8Z3_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0824762.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="ACG8Z3_002505"
     CDS             complement(1386..1673)
                     /locus_tag="ACG8Z3_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0824763.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="ACG8Z3_002506"
     CDS             complement(1663..1992)
                     /locus_tag="ACG8Z3_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0824764.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="ACG8Z3_002507"
     CDS             2297..2626
                     /locus_tag="ACG8Z3_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0824765.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ-A"
                     /locus_tag="ACG8Z3_002508"
     CDS             complement(2913..3398)
                     /gene="vanZ-A"
                     /locus_tag="ACG8Z3_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0824766.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="ACG8Z3_002509"
     CDS             complement(3551..4462)
                     /locus_tag="ACG8Z3_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0824767.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="ACG8Z3_002510"
     CDS             complement(4890..5498)
                     /locus_tag="ACG8Z3_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0824768.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="ACG8Z3_002511"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="ACG8Z3_002511"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0824769.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="ACG8Z3_002512"
     CDS             complement(6528..7496)
                     /locus_tag="ACG8Z3_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0824770.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2350 a         1532 c         1281 g         2536 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ttaggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatt
//
LOCUS       DAZKSU010000101         7627 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000101 DAZKSU010000000
VERSION     DAZKSU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.104"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..712)
                     /locus_tag="ACG8Z3_002513"
                     /pseudo
     CDS             complement(14..712)
                     /locus_tag="ACG8Z3_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            791..967
                     /locus_tag="ACG8Z3_002514"
                     /pseudo
     CDS             791..967
                     /locus_tag="ACG8Z3_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="ACG8Z3_002515"
     CDS             complement(1143..1712)
                     /locus_tag="ACG8Z3_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0824771.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ACG8Z3_002516"
     CDS             complement(1786..2109)
                     /locus_tag="ACG8Z3_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824772.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ACG8Z3_002517"
     CDS             complement(2263..3075)
                     /locus_tag="ACG8Z3_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824773.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3402..4277
                     /locus_tag="ACG8Z3_002518"
     CDS             3402..4277
                     /locus_tag="ACG8Z3_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0824774.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4291..4614
                     /locus_tag="ACG8Z3_002519"
     CDS             4291..4614
                     /locus_tag="ACG8Z3_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824775.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4611..5363
                     /locus_tag="ACG8Z3_002520"
     CDS             4611..5363
                     /locus_tag="ACG8Z3_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0824776.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ACG8Z3_002521"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ACG8Z3_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1371 g         2472 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aaccttt
//
LOCUS       DAZKSU010000102         7597 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000102 DAZKSU010000000
VERSION     DAZKSU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.105"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..425)
                     /locus_tag="ACG8Z3_002522"
     CDS             complement(135..425)
                     /locus_tag="ACG8Z3_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824777.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="ACG8Z3_002523"
     CDS             complement(543..803)
                     /locus_tag="ACG8Z3_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824778.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="ACG8Z3_002524"
     CDS             complement(940..1353)
                     /locus_tag="ACG8Z3_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0824779.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="ACG8Z3_002525"
     CDS             complement(1809..2294)
                     /locus_tag="ACG8Z3_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824780.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="ACG8Z3_002526"
     CDS             complement(2433..2648)
                     /locus_tag="ACG8Z3_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0824781.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="ACG8Z3_002527"
     CDS             2872..3672
                     /locus_tag="ACG8Z3_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0824782.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="ACG8Z3_002528"
     CDS             3691..5559
                     /locus_tag="ACG8Z3_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0824783.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="ACG8Z3_002529"
     CDS             5552..6043
                     /locus_tag="ACG8Z3_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0824784.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /locus_tag="ACG8Z3_002530"
     CDS             6031..6585
                     /locus_tag="ACG8Z3_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0824785.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="ACG8Z3_002531"
     CDS             6603..>7597
                     /locus_tag="ACG8Z3_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0824786.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt ttccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DAZKSU010000103         7183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000103 DAZKSU010000000
VERSION     DAZKSU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.106"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(979..1152)
                     /locus_tag="ACG8Z3_002532"
     CDS             complement(979..1152)
                     /locus_tag="ACG8Z3_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824787.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1155..1553)
                     /locus_tag="ACG8Z3_002533"
     CDS             complement(1155..1553)
                     /locus_tag="ACG8Z3_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824788.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1769..2824
                     /locus_tag="ACG8Z3_002534"
     CDS             1769..2824
                     /locus_tag="ACG8Z3_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824789.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2844..3197
                     /locus_tag="ACG8Z3_002535"
     CDS             2844..3197
                     /locus_tag="ACG8Z3_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824790.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3379..3666)
                     /locus_tag="ACG8Z3_002536"
     CDS             complement(3379..3666)
                     /locus_tag="ACG8Z3_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824791.1"
                     /translation="MKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAKLKS
                     DSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3679..4497)
                     /locus_tag="ACG8Z3_002537"
     CDS             complement(3679..4497)
                     /locus_tag="ACG8Z3_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0824792.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4723..4944
                     /gene="relB"
                     /locus_tag="ACG8Z3_002538"
     CDS             4723..4944
                     /gene="relB"
                     /locus_tag="ACG8Z3_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0824793.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4946..5212
                     /locus_tag="ACG8Z3_002539"
     CDS             4946..5212
                     /locus_tag="ACG8Z3_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0824794.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5379..5864
                     /locus_tag="ACG8Z3_002540"
     CDS             5379..5864
                     /locus_tag="ACG8Z3_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824795.1"
                     /translation="MGWYGDYSSANEVIEEKKGYAKNGLEIVGTKVTKTYGIILFKDA
                     EGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFFVGDQEKMVKLQLDRIKEE
                     SNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQDGSLTRFTRLSAGDVKEV
                     T"
     gene            6073..6255
                     /locus_tag="ACG8Z3_002541"
     CDS             6073..6255
                     /locus_tag="ACG8Z3_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0824796.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            6358..6642
                     /locus_tag="ACG8Z3_002542"
     CDS             6358..6642
                     /locus_tag="ACG8Z3_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824797.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6658..6894
                     /locus_tag="ACG8Z3_002543"
     CDS             6658..6894
                     /locus_tag="ACG8Z3_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824798.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6916..7170
                     /locus_tag="ACG8Z3_002544"
     CDS             6916..7170
                     /locus_tag="ACG8Z3_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824799.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
BASE COUNT         2492 a         1188 c         1107 g         2396 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac cttgcgtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgaacaagt ttttacctat tcccaaaatt aatttattcg ttattttttc attgattaag
      181 aataattatt tgatgcttaa attagctaaa aaaacgattt tttactgata taatcatgat
      241 attaaagctg aattaggcgg aaagataaaa tgttcgcaaa atcaaagtat caattatctt
      301 gtttttttat aaatttgtaa gtaaaaatct aaaaagtttt cttttaattg gtatatgtat
      361 taaaatcaca ttcaatcact cataaaagta tgcgctttca ttttttttgg atgagattaa
      421 tatcaataaa taacgatgta aaaaccatta attatgctct tttataagga tattaattaa
      481 tctaatttga aaaaataatt caaaatttaa atgttttaat attacaaata aaatcatgtt
      541 atcgtaaaat ggtataaaaa agctaaatat tctatttttg ttattttatt agcagggaaa
      601 tttaaatagt ttacaagaat ccgtagaaat atattggttt tgccttcaga ttaatactgg
      661 atcatattaa tctgaatata atatatgaac taataaatag cttaacctcg ctattttgtt
      721 ttttctgatc actatctata cagcattagt aaatagaatt ttaaaaataa aaaaattaag
      781 acaaaaaaag tatcaacata aacatataca tatacacgtt tatgttgata cacttaattt
      841 gtcctttaaa aagcacttat tttaatatta tgttatttta taatcttatt acaagctgat
      901 tgttagacgt tgtcctttca ttacatttat taacggttat tttatcgttt aatttattaa
      961 ttatttttga tctaaaactt agcttaacaa ataaataatt aaccaaccgc cagataaaaa
     1021 tgcgatcatt gctaatacat aagcaaagat aaaaatatgt tcgatcagct taaaccctga
     1081 tgcgattaag cagaccaagt aaaatagaaa ttctcttaca gataatggta attttctaac
     1141 tagcatactc ataattaaca ccccaaagta atattgtatt ttgaacattt tacatccaat
     1201 acagcatcaa tcaaccattg ccaatcaaaa atacaaggct tcattgaagt atgtttttca
     1261 gatgaaaaga tataatataa tttcttcgga ataatatttt cagttgttgg gatcaagcta
     1321 aatttaatac catctaactc atagctacca gctttctctc cattaaaaat ttccttagca
     1381 ctttcttcta aagaatcaaa atatttttcg acagcaacct ttgaagcttt tactattgaa
     1441 gcatttccct tttcgataaa atctgctttt gttttaccga caataagtaa ttcttgatca
     1501 gtaaaatctt ctaaaataat agtagtgtaa ttcttatttc ttctcaatcg catcaggatc
     1561 actccttaat ataatttatg cttttataat atcataataa gataaatttg caagtgttta
     1621 tttacacctt tttatttaca ctttattgta attgattata cttttttgat tgcatttttt
     1681 tcttataaac agttgcaact attagtaaat aggtgtaaac tattttcgta ccgaaatacc
     1741 ataaacacaa acagaaagag gtcataccat gaaagaacca ttattgtttt ccgttgactt
     1801 agggaataaa tcagtaaaag aaatgagtac acacgtttct ccactctcgc ttccctctcg
     1861 tattttgaac aaaaatatca ttaattcagg acgtttttct aatataggtt cctcattaaa
     1921 acgatcacta aacgtttctg aatatagcct agtaaacact actgaaagct attattttgg
     1981 gaaggatata cacgagctag gaaaagatga ctacgtgata gaatcactag gttttgggat
     2041 cagcagatac aagcaggaaa cctaccaaga tatcctatct ttctctttag ctaacctagc
     2101 acgtttagaa gatcaatctg atgacgtttt aaccgttaat ctagttttag gtctgccttc
     2161 atccgactat accgaaacaa tcgtcaaata cgtgatgcaa caagccacac gacaacattc
     2221 agttgaagtt gacggtaaag cttataatat aaacgtagat accgtttggg tactgccaca
     2281 gccgattgga acactatata acctcctcct tgctgatgat ggtagtgtac aagatgaatc
     2341 attgatcagt cagaagatcg gttttgttga tattggtggt ggcacgaaac tttttgatgt
     2401 tgtccaaaat tttgaaattg acgaaaacaa ccgtgaacaa aaggataccg gtatttttac
     2461 tttatataat cgtatcgtaa atatgcttga ccttccgatc agtcctaatg ctttccaact
     2521 tgaaaagatt atccgagaag gcaaagaaaa aggcgtatat ctatttaaac caagcggaaa
     2581 tgaaagtttt gatatttcag aagtagttga aagagaaata aaaacataca ccaatatggt
     2641 tgttgcagaa gttttagcta cattcaaaaa cttcacaacc ttagataaag ttgtctttac
     2701 tggtggtggg gcttctatta tcgacaaaca acttgtaaaa gataaaattc cgcatacctt
     2761 gtttgttgaa gacagtgaat tttctaatgt aaaaggcttt tacaagtttg gtttaactca
     2821 ataaaacagc gaaggagatc aacatgaata agggaaaatc agagcgttat gtagttataa
     2881 tcaatagaga aaaggttagc gagttacctt tagcagagtt tcttgaatct gaatatgaaa
     2941 cactaggcgt taaatactct cagacgatca aaaaagcttt gaatctatat cggcgtgtaa
     3001 agaacaatga agttacaatt attgaagcag gcaacgatag aaacgatagc aaagtagctg
     3061 aagtaaagaa taaaccaact gatcataacc ctattcaaca agcgcatcct tcaacggtta
     3121 catccgatca ttcagcaaag aaagaaaagc caatgctaaa agggttcaat gcaccaagta
     3181 acaaagactt actttaaaaa taaaaaggcg aggttacaat acctagcctt tttattatgt
     3241 acaaatacaa acataaacat atacgtatac atgtatataa acatgtatac gtatatatcc
     3301 ataaacacgt atacgaaatt tagttaagct atacctaacc gcttattcca ataaaaaaag
     3361 tgatcagctt taaactgatc actcaccaaa aacttcatca tacaagttat ctatcatttc
     3421 accaagtaaa gctgaatctg atcgaattcc taacttttcg ctgattttag atagtttctt
     3481 tctttgtgat ggttgaagcg tgaattgata atttttcttc ttttcagaac tatcactttt
     3541 tagtttagct accaaagaac ttgttgtttc tttaggtgtt tgtttaggtt cttcaactgc
     3601 ttcaaccact tcaggaactt cttctacttc agcttcagcg aattttttaa atctattttc
     3661 tttcacttca atcactcctt attcttgtgc cgtatcaatt attttgttgt atagcgtaaa
     3721 cgtgctttca aagaaatcct tattcttacg atagatattc ttatccttag cgaactcagc
     3781 aatattctta ccttctaagc tagttgcatt aagcaattcc ttctcttgta catatccaag
     3841 ataattagat aacgttggta ccaattctag gaactcacga cttgaattag tattatgttt
     3901 gatcctgcta ccgatcgtag cgtatttagt ttccatcagt gtttcaccag tcttaatatc
     3961 aatcaactgt tttgctagtg catcaatatc tttaccaagc ttaagcaaag cttcaaaacc
     4021 atctttagat ggatcagaaa tgcctacgac aagatcagat acagcccaag cattttgagc
     4081 tagtagacct gcatcattat gagtatcaat cagaattaga tcatacttct ctttaagctg
     4141 ttcatagtta acaacgatcc atctaaacag tagggttctg ttgttaggca tatcacgcat
     4201 ttgcatctca acttgtttta aatcttcacc tgctactaaa atatccatgt tctcatttat
     4261 agctaaaggt tcagctgatc catgaagata taaatgattg attgtattct ctcttttcgc
     4321 tttactgaaa gcatcaggaa atcgttttga taggttttgc gacttatctt catcaatata
     4381 cagcaccttc atacctaaat tttcgacagc atatccacct agattgtaga ttgttgttgt
     4441 tttacctgtt ccaccttttt caatgtttcc agtaatgatt gacaacttct tttccatgat
     4501 taaacctccc ttattaatct tttgtaactc cattatacac tataaacacg tttatgtata
     4561 tatttatata cataaacgtg tttatattta tatttgcttt tatattctac aacataaaca
     4621 tatatatgta tatgtttatg tttatgtata tattcaaata tataactatt gcatttgtca
     4681 tacaatcgta gtacaatgta attgaaaagg agttgataaa ctatgccagt aataagtatt
     4741 cgtgtatcta aagaagaaaa agattttctt gaaaaagttg ctgaattcaa cggagataac
     4801 atttctgaat ttatgcgtag taattccctg caaacagcag aaacgttagt agactttgcc
     4861 acctacaagc aactaatgaa agagcatgag gaaaacgatc aaagtatttc ccacgaagat
     4921 atgctaaaag agttaggctt ataagatgac ttaccaagta agatatgaac gtgaagctca
     4981 aaaagcgcta aaaaaaatgg ataagtttca ggcaaaggtt atcctaaact ggatcgagaa
     5041 aaatcttgta ggcactgatg atccaagaaa gcatggtaaa ggtctaacag ctaataaatc
     5101 aggctactgg cgatataggg taggtgcata ccgtataata gctgatatta gcgagcatga
     5161 agtgacaatt cttatattgt ctattggtca cagaagtgaa gtttataagt aacagacaag
     5221 ctgataaggc ttgtctgttt ttttgttaac ccaacaaaat cactgtttac gatcccactt
     5281 tacctaaaaa agaacaatat cctcttgata ttatacacac cagtgtgtat aatataagtg
     5341 taaacaagct aattaacttt caaaaggagc gattttttat gggatggtat ggtgattatt
     5401 catcagcaaa tgaagtgatc gaagaaaaga aagggtacgc caaaaatggt ttggaaatcg
     5461 taggtaccaa agtaacgaag acctatggaa ttatcctttt caaagatgca gaaggaacct
     5521 acgatattga cagcttcatc tttcgagatg aaatgtacaa accaatctac ttcttaaatg
     5581 acagtcctgc ttactttaag aaaattccga aaaaatggtt gaaattcttt gtcggtgatc
     5641 aagaaaaaat ggttaagcta caactagata gaattaaaga agaaagcaat aaaccaaaac
     5701 ttgatgactt attagttgca ggtaaaactt actcaatctg ggatggatcg tatgtcgctg
     5761 tctatcagta taaactcaaa cgtagcttta tttttcgact acaagatgga tcactcactc
     5821 ggttcactcg actatcagca ggagatgtta aggaagtaac ctaaaactta aatacataaa
     5881 cataaacgtg tatacgttta tgtttatgta tatgtatatg aatataaacg tattaggtac
     5941 gaacactttt aaattctttt ctgggctttg tttttagggt agggctaaaa aaaacatcac
     6001 catctgatcc aaaacagctc agaaacgcat ttaaacgtat ctgataccaa tattacagaa
     6061 ggagtgtatc gtatgattaa cctatttctt ttagtgacag ctagtacatt attctttact
     6121 ggaatatcac tgataatttc ctatttgatc tataagaaag ccgaaaatca cacaagcaaa
     6181 gcaatcggac tagccgtttc atccttttta actttgctga ctttatttgt tactttatcg
     6241 ttttcttttg tttagaacag ctgatccatt agcacctttt tctctttaca ttatacacac
     6301 tgttgtgtat aatgtatttg taaacaagct aattaacttt taggaaggag tgacaagatg
     6361 ctaacagcta ctttacagca acctactcac gttgctattg cagaaagtta tacaattaat
     6421 gcagggatcg ttaaacagct gatcaaggat cgagaattca aaaagtttaa cagtcggcaa
     6481 gcgtataaca gacagctaaa gaaagctatt ctaaagcaca aagattcctt catcgggaac
     6541 ttggaaagtg tagatgttaa aactgtttca acggcatttt cagttgttga agtgactatt
     6601 gacggtcgct tgatttctct atttgctttt ggaattaatt gatagggagt gtgaactttg
     6661 gaaggtaaga cttttgcaga aatttggact gaatttattg aggtaccaac tcgcattgat
     6721 ccgataactt ctgaaaaggt attggatgca gacttctaca atctcaaagc aggtactagc
     6781 cttgaatgcg tttggatgta tttagaagag catttcaaag gcgtaacgct cggtcacgtc
     6841 ttataccaca atggtgccga gtgggatgca attggaaata tccatttttc ttaatataag
     6901 aaaggagaac acaatatgaa ttataccgat gaagaacaaa tcgctttgga atacgttttc
     6961 acaaatgaag aaggggagga aattgaaaaa gtcttgatca tttcatcaaa agacttggag
     7021 aaggctttgg cttatgaacc atacgaatca accgtaactg tcattgaatt cttagatacc
     7081 tatacttcta atgatacttg ggaaattatg aatatttgca acgctaataa cattcaattt
     7141 gctgaaaaaa ccaataaatc attttattag ttttgcaata aaa
//
LOCUS       DAZKSU010000104         7035 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000104 DAZKSU010000000
VERSION     DAZKSU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.107"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      229..403
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            541..2133
                     /locus_tag="ACG8Z3_002545"
     CDS             541..2133
                     /locus_tag="ACG8Z3_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0824800.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            2146..2886
                     /locus_tag="ACG8Z3_002546"
     CDS             2146..2886
                     /locus_tag="ACG8Z3_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824801.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            3054..4403
                     /locus_tag="ACG8Z3_002547"
     CDS             3054..4403
                     /locus_tag="ACG8Z3_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0824802.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(4531..5121)
                     /locus_tag="ACG8Z3_002548"
     CDS             complement(4531..5121)
                     /locus_tag="ACG8Z3_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0824803.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            5268..6620
                     /gene="brnQ"
                     /locus_tag="ACG8Z3_002549"
     CDS             5268..6620
                     /gene="brnQ"
                     /locus_tag="ACG8Z3_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0824804.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         2099 a         1286 c         1428 g         2222 t
ORIGIN      
        1 aattttctgt tctagttctt tttttattgt attacataca tttttgtgta taattatact
       61 atcgtttgca atggatagat gattcacatt ttgattgttt tttcggtaaa agtaaagatt
      121 tctatattta tcagtatttt ttcaaaaacc agcagttata gattgacaaa gcctctcgag
      181 tatcgtaaac tttttttgta taaataactg aatacgagat gcctcaatca atgaggtaga
      241 ggtcgcgaga atcattaacc ctgtggagtg aagcaaacat ggagaagcag gaatagggag
      301 gatcgccgaa atgtgcactt ttttgcttgg agtgtatgtt gggacgcaag ttaagagctt
      361 gcggactgtc ttagcagtga tgctaagttg cgctatgttt tgtgagaggt ttatcacctt
      421 atggaatttt agcggccttt ttatatctat aaatcggtcg ctttttattt tttcagataa
      481 agctttaccg catttcatct agaccgctcg tgtatcatat aatcagaaag ggagaaaaaa
      541 atgaaaaaaa atatggtttc agttgccgta ctatttgctt ttgtcttctc actattcagt
      601 gtgggatctc tggcaaatgc agatgaaaaa acaccgagcg atgagtttcg cgtaggtatg
      661 gaggctgggt atgcaccctt caactggtct caacaaacag atgagaataa tgccttgcca
      721 attcaaggcc aaaatagtta cgccggcggt tatgatgttc aaatcgctaa aaaagtagca
      781 gatggtttag ggaaaaaatt agtcatcgtc caaacgaaat gggatggtct ggctcctgcc
      841 cttcaatcag gcaagatcga cgcgatcatc gccggaatga gccctactgc tgaacgacga
      901 aaagaaattg attttaccga tccttattac gaatcacaat tagtagtagt agttcaaaaa
      961 gatagcaaat atgcaaaagc aaaaagttta gcagatcttt ctggagcaaa gatcactgct
     1021 cagttaaata cattccacta tactgtgatc gatcagatac cagatgtgca gaaacaacaa
     1081 gcaatggata atttctcagc aatgagaaca gctttagctt ctggaatgat cgatggttat
     1141 gtcagcgaac gtccggaagg tgtaacggca actagtgtga acaaagactt ggaaatgctt
     1201 gaattcagcg gtgcagatgg cttccagaca aacccggaag atgttcagat ttctgttggt
     1261 atgcgtaaag gcgacccgga tatccaaaaa gtaaaccaga ttctttctgg catctctaac
     1321 gatgaacgaa ccaaaattat ggaccaagca gtcaaagatc agcctgctgc aactgatagt
     1381 gaagaaaaac ctggcatcat ttctgatttc actacgattt ggaaacaata cggcaatatg
     1441 ttcttacgag gtgctggact taccttgttc atcgcattga tcgggacagt tgtcggtact
     1501 acacttggat tactgattgg tgtcttccgt tcggtacctg aatcaggaaa tgcactgact
     1561 cgcttcttcc aaaaaatcgg aaatgcactt ctttctatct atgtcgaagt attccgcgga
     1621 acaccgatga tggtacaagc aatggtcatc ttctacggtc tggcactcgc ttttggcatt
     1681 tcattgaacc gaacgattgc cgctttgttt attgtctctc tcaatactgg agcttacatg
     1741 acagagatcg ttcgtggggg gatttttgca gtagatgaag ggcagttcga agctgcgcaa
     1801 gccattggta tgacccatgg ccaaacgatg agaaaagttg ttattccaca agttttacgg
     1861 aatatcttgc cagcaacagg aaatgagttt gttatcaata tcaaagatac agcagtttta
     1921 agtgtaatcg gcgtagctga tttgttcttc caaggaaact ctgcttccgg tgcgaatttc
     1981 caattcttcc aaacatttac gatcgtcggg atcatctact tagtcatgac ctttacgatc
     2041 accagaatcc tgcgggcggt agaaaagaaa atcgatggtc cttcagctta taccaagctt
     2101 gaagaaatcg acaattcaaa tttgcaggaa taaggaggaa gaatcatgaa tgaaaaaatt
     2161 attgaaatca aacatctaag aaaaagcttc ggtgaaaatg aagtattgaa agatatcagc
     2221 gtaacagtca ataaaggtga agttgtgacg atcatcggtt cttccggttc tggtaaatct
     2281 actttcttgc gctgtatcaa tttactggaa aaaccaacag gcggcgagat catttataat
     2341 ggagaaaatg tattagctcc taaatataat ttacctaaat atcgaacgaa tctaggtatg
     2401 gttttccaat ccttcaacct gttcaacaat atgaatgtct tggaaaactg tatgtctggt
     2461 caaacaactg tcctgaaacg agataaagaa actgccaaaa aagtagcaat cgaaaatctg
     2521 gaaaaagttg gtatggatcg ttatatcgaa gccaaaccag cacaattatc tggtggacag
     2581 aaacagcgtg tcgcaattgc tcgggcatta tcaatgaatc cagatgtatt gctattcgac
     2641 gaacctactt cggcgttaga ccctgaaatg gttggcgaag tgcttaatac gatcaaagac
     2701 ttagcacata ctggcttgac aatgcttatc gtgacgcatg agatggaatt tgcaaaagat
     2761 gtatctgacc gtgtcatttt tatggataaa ggtgtgattg cagaagaagg tacaccagaa
     2821 gatatctttg tccatccgaa agaagaacgt acaaaagaat tcctttcacg tattttaaat
     2881 gcttaggatc aatagctgat tgggacaaag agctgcggtg tttaccgcag ttctttttta
     2941 tgaattactc ataaaaggta aatttctgaa aattttggaa tcttagttgt gtttttcaga
     3001 aaactctctt aatatggaag taattaaact tttttagtta ggatgattta aaaatgaatg
     3061 aagagcaaga actgcagcgt ggactgaaaa atagacatgt ccaactgatt tctattggcg
     3121 gagcgattgg aacggggttg tttttaggtt ccggaaagtc tatccagcta gctggccctt
     3181 cgatattgct cgcctatctt attactggat gcatctgttt tttcattatg cgtgcgttag
     3241 gcgaacttct gttatcaaac accaataacc attcttttct ggattttgtt gcagaatatc
     3301 ttggcaaaaa agcggctttc atcactggct ggacgtattg gttctgctgg gtatcgattg
     3361 caatggccga tttaacagcc atcggaatgt atgtacgcta ctgggcgcct ggtgttcctc
     3421 agtggcttcc tgaactgatt gcattaggtc ttcttctttg cttgaatatg gtggctgtct
     3481 cattgtttgg tgagttagag ttttggtttg cattgatcaa agttgtagcg atcattgcct
     3541 ttatcctcgt aggagcttat atgatcctca cccactatac gacagatatt ggcacagcca
     3601 gtatcacgaa tctatggtct catggcggat tctttccaac tggtgcaaaa ggatttatct
     3661 tagctttcca aatggtaacc tttgcttttg ctgggataga attagttgga ttagtcgctg
     3721 gagaaacaga aaatcctgaa aaagtgttgc cggaagctat caataatatt cctatcagga
     3781 tcatcttatt ttacttagga tctctttttg tcattatggc aatctatcca tggaacagct
     3841 tgaatccaga taacagtcca tttgtagaag tattttcgga aattgggatc acgattgctg
     3901 cttccttgat caatttagtc gtattaagtg cagcagcttc tgcctgcaac agtgcaatat
     3961 atagtacagg acgtatgctt cgttctttag cgcaagaagg cagtgcccct aaaaaattca
     4021 aaaagttaac aacacatcat gtgccaggga atgctttgac attttctact atcgtcattt
     4081 tcatttctgt tatcttgaat tacgtgatgc ctagtgaagt attcacacta gtaagcagta
     4141 ttgccactac ttgtttcttg tttatctgga gtatgcttgt ctacacgcac atgaaatatc
     4201 gtaaaagtat cctcggcaaa aaagctcatt catttaaaat gccgttgtac ccatttagta
     4261 attatctggt atttctttac atggcctttg tgtgtgtcgt cttgttccta ggaaaagata
     4321 cgagaatcgc tttattacta acacctgtgt ggttcttgct tctattacta atctatcata
     4381 tgaaatatga acgtaagaag taagaaagta gttgtgacag aagtggtcag cttcgagaaa
     4441 taagaaggaa tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttcttg
     4501 gagataaaag cttctgtccc agcctctttt ctatttcccc ggaaacaacc gctgataata
     4561 ggcatctgct tttttatagg aaaaaatcca aataccagat acaaaaaaac gtccgaataa
     4621 cccgatcaac ggtacaaaaa agacaccggc aatcaagcat aagttaatca tcatggagat
     4681 aagacttcga cggtcctttg acaaaactct cacgacatgg gaatctggtt tatttgcttt
     4741 catgatttgc ctcatcaaga taccaaaaga aaaactccac atcgtgtata ctaagacata
     4801 agccatctca ggccaaaaag cttcagggtt acgcccgacc caactagtaa cagaaggaca
     4861 taaagtcagc caaaagagca aaaagatatt cgaccagacc actcggtaat taattcgttc
     4921 tgccatctga aatatacgat gatggttcac ccagatcacc gcaacaaata taaagctgat
     4981 ggtataagct aataatgtat ttcggatgac ccataaagaa tgaaagctag atgtattagg
     5041 gatcttgatc tctaatacaa taactctcaa aacaatggct accactccat cagtaaatgc
     5101 ttctaatctc gactttgtca cgatgattcc tcctcagatg attcagcgct tttattttat
     5161 cacttacgtt attaaataac catcgtttta tatagggaat tttctgaaaa ttgttgcata
     5221 tttttcgaaa ttttgttact tttaaaaaaa ccgtaaagga gttatgtatg acaaaaaaat
     5281 tatcatttaa agactatctt tttataggct ccatgctttt cggtctattt tttggtgctg
     5341 gaaatcttat ctttcccgtt catttgggtc aagaagcagg cgctgctacc ttttgggcga
     5401 atctaggttt cttggttacc ggtatcggtc ttcccttctt aggggtgatt gccattggtg
     5461 tatcgaaaag ctcaggtgta tatgaattag caaaacggat aaatcaaaca tatgcgatgg
     5521 tttttaccat cttgctttac ttagttatcg gaccgttttt tgctttacct cgccttgcaa
     5581 ccacatcttt cgagattggt ttagcaccat ttatcgatcc tagcagacag acgctatatc
     5641 tagcaatttt cagtattgta ttttttgtgc tggcttggtg gttttcaaga aaaccgacaa
     5701 agatattaga ttatgttgga aagttcctga atcctgcatt tttggtttta ttaggtgctt
     5761 tgcttctcct tgcttttttg aatccactag gttctgtaaa tcaagcgcct attcagccaa
     5821 attatcaaga gcatgctttt ttcacagggt ttactcaagg atataacaca ttagatgcgc
     5881 ttgctgcttt agcatttggg atcatcattg tcacaacaat ccaaaacatg ggtgtaacaa
     5941 aacctgctga aatcgctaaa gacacgatca aatctggtgc gatcagcatt atattgatgg
     6001 gaatcattta tactttgctc gcttatttag gtgctatgag cttaggtagc tttgctctaa
     6061 gtgaaaatgg cgggatcacg ctggctcaga ttgctgatca ttatttaggt acttatggca
     6121 gtatcttatt agcatttatc gttatattag cttgcttgaa gaccggtatt ggtttgatta
     6181 cagcttttgc agaaacattc acagatttgt ttccgcaaag aaattacgca ttctttgttg
     6241 cacttgctag tatccttcct tgtttagcgg caaacgtagg cttgacgaat atcattcaat
     6301 tttcgttgcc tgttttgatg ttcatctatc cattagcaat gacactgatt ttactagtgt
     6361 tagttggtcc tttgtttaag cagcgtacgg ctgtttatcg aatgactact tattttacat
     6421 tgattgcttc gatttttgat gggttgaacg cttgcccaga aactatcaaa caaacaccta
     6481 ttgttcaaaa tcttttatat gttgcggaaa gctacttgcc gtttttcaaa ttggggatgg
     6541 ggtggatcgt accagcagtt atcggttttg ttatcggact gatttggagt ttcgctaaga
     6601 aagaagaagt agcagattga taaaagaggt tgtgaaagag acgttcagcc ttgagcaata
     6661 aaaagtaatt ttgaaaaatg gcttctcaca tttttgcata aatttgactt aataccgaga
     6721 aactgacttt tgaacaccgt ttattgagag gtcgtgaaaa agccgcttag tttcaagtat
     6781 taagaaatat tttgtgaaaa tggcttctca catttttgca taaatttgac ttaataccga
     6841 gaaactggct tttgaacacc gtttattgag cgtctataaa ctgacctgag tattagagaa
     6901 acaaaacggc aaagtagctc catatacttt gccgttttgt tttgaactaa taccgaagag
     6961 aagctgatca aacaacattt cttttataat atagatttta gacatatata tgatataaaa
     7021 atagttttcg caaaa
//
LOCUS       DAZKSU010000105         6905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000105 DAZKSU010000000
VERSION     DAZKSU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.108"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG8Z3_002550"
     CDS             complement(<1..182)
                     /locus_tag="ACG8Z3_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0824805.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG8Z3_002551"
     CDS             517..951
                     /locus_tag="ACG8Z3_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0824806.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG8Z3_002552"
     CDS             974..1294
                     /locus_tag="ACG8Z3_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824807.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG8Z3_002553"
     CDS             1435..1626
                     /locus_tag="ACG8Z3_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824808.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG8Z3_002554"
     CDS             complement(1697..1954)
                     /locus_tag="ACG8Z3_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0824809.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG8Z3_002555"
     CDS             2112..2375
                     /locus_tag="ACG8Z3_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824810.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG8Z3_002556"
     CDS             2399..2587
                     /locus_tag="ACG8Z3_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824811.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG8Z3_002557"
     CDS             complement(2670..2855)
                     /locus_tag="ACG8Z3_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824812.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG8Z3_002558"
     CDS             3070..3441
                     /locus_tag="ACG8Z3_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0824813.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACG8Z3_002559"
     tRNA            complement(3591..3663)
                     /locus_tag="ACG8Z3_002559"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACG8Z3_002560"
     CDS             complement(3709..4137)
                     /locus_tag="ACG8Z3_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824814.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACG8Z3_002561"
     CDS             complement(4197..4955)
                     /locus_tag="ACG8Z3_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0824815.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACG8Z3_002562"
     CDS             5250..5447
                     /locus_tag="ACG8Z3_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824816.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACG8Z3_002563"
     CDS             5553..6035
                     /locus_tag="ACG8Z3_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824817.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACG8Z3_002564"
     CDS             complement(6093..6689)
                     /locus_tag="ACG8Z3_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0824818.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2338 a         1061 c         1356 g         2150 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaattctag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt atttgaaaaa
     6901 aacag
//
LOCUS       DAZKSU010000106         6593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000106 DAZKSU010000000
VERSION     DAZKSU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.109"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..978)
                     /locus_tag="ACG8Z3_002565"
     CDS             complement(31..978)
                     /locus_tag="ACG8Z3_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0824819.1"
                     /translation="MSERISYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(1156..1959)
                     /locus_tag="ACG8Z3_002566"
     CDS             complement(1156..1959)
                     /locus_tag="ACG8Z3_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0824820.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            2105..3019
                     /locus_tag="ACG8Z3_002567"
     CDS             2105..3019
                     /locus_tag="ACG8Z3_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0824821.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            3067..3537
                     /locus_tag="ACG8Z3_002568"
     CDS             3067..3537
                     /locus_tag="ACG8Z3_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0824822.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            3622..4230
                     /locus_tag="ACG8Z3_002569"
     CDS             3622..4230
                     /locus_tag="ACG8Z3_002569"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0824823.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            4364..4564
                     /locus_tag="ACG8Z3_002570"
     CDS             4364..4564
                     /locus_tag="ACG8Z3_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0824824.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            5318..6544
                     /locus_tag="ACG8Z3_002571"
     CDS             5318..6544
                     /locus_tag="ACG8Z3_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0824825.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
BASE COUNT         2228 a         1001 c         1317 g         2047 t
ORIGIN      
        1 tttttctttt gaaaatattg ttttcagcta ttaatatcct gcagacaatt ttacttgatt
       61 ggtttgaagc tcatgggtgg caaagtagct tttcagattg gttaaaaaaa tagccattaa
      121 tttcttttga aaatcaggtg tcaaacctgc aatgtgggaa gtaatcagta cattgtccat
      181 tttccaaaga ggatgatctt gtggaagagg ctcttcctca aaaacgtcta atgctgcaaa
      241 ggaaagttgc tggttattta aagcctgcac cagatcttgt gtcttaacag aagcaccacg
      301 gccaacattg atgaatacag attttggatc aaagtgttca aacatatgct gatcaaagag
      361 cccagttgtt tcctccgtta gaggtaagat attgactacg aaatctactt caggaagaac
      421 ttcctttagt tgatttaaag aaaaagtctt ttccattcct tctgctggat gaccggatgt
      481 attgattcca tagcagttca tgtctaatgc tttgcatttt tttgcaatca tttttccaat
      541 atggccagtt ccaatgatta gtattttttt cccttggata gattggtaat ggaccgatga
      601 acctaaccat ttcatgtcac tctgtgcctt ttgtgcttga aaaagaccac gagaataccc
      661 aaggatgatt cctaagacat gctccgtaat cgattcactg tggatgccac taccatttga
      721 aagtaaaata tgtttctttg aaaaagtctc aaggggtagc tgatcaacac cagctgagat
      781 agattggacc catttcaagg tagaagaggc taatagtttt tcttggtagt ctgagttcca
      841 gcccaaactg atctctacat tttgtagatc ctctggttgt aattctgttt taaagatata
      901 accaggcgct atagtatgga tttcttcgat aaattcatca cgaaatggac gatctgcata
      961 actgattctt tctgacatag ttaatttccc gcctttcttt actctaagtg tagcgcatac
     1021 aagaagaact ttgaagcgcc ttgcttgttt tttgagaaag aaatattttt caaataaaaa
     1081 aggggctgtg acaaaaaaat gtcacaacct ctaaaagggt ctattgaagt agaaataacg
     1141 agttgtcttt atttcttaat tgatcgatat ttttgcccat cctttgacag cttgagcaaa
     1201 aataaagatt cctgcaaaag caatgaaggc gctagtgtca aaagaaggaa gaaagaccat
     1261 caagattgtt gcaattatag taggcaatga aattgaatag atcaatgttt ttaaacaatc
     1321 aagaaaagta gcttttgtta gtcgaaagcg tgcatagaga aaagcagcaa tgttggtaaa
     1381 tagcaaagtg atcagcagat tcagaaaact aggataaata gaaaatataa atgtcaggac
     1441 ttttatccaa agtggtatag atgcttcact taaaaaagaa cggagctgct tacctgttaa
     1501 gttttttaaa gattcattgg tgtagggaag ttctaattgg ttattgttca acaaagcaga
     1561 agtcgtgcct gtattaggaa gagctatgat aagtttgttt tttaacatcc cgacactaac
     1621 aaaattcccc atcaaatcag tagagatgtc ctgttctgtt cgttttccct ctggatcaaa
     1681 tgtgaagata atggagtttg tttggtaaat gaatccttct ttttcttcgg tttgaagctg
     1741 cccgttttgg attgtaaaat caggaatgcg agtagcaatc ttttgcccat ccaattgaat
     1801 gttgtttaag acttgaaaaa cttgaaaaga gattggcaaa gccataatta tactcaaaac
     1861 taaaagatag acaatggctt tccaaaatgg gacatttctc gcctccttta ggtctttgaa
     1921 tcgtataaag gaactgataa ttaattgttt tgttgtcata agtactcctc atctagtagt
     1981 gtcatcactt gcaattctag cgaagtatca agtgaaatac aagtgcttta aggaaacagc
     2041 tgaatgattg gtgaaatatc aaaaaaatgt tattcttgtc taaagactat ccaatataaa
     2101 taaaatggag gaaggaatag tgcctacaca gccacagaga tcatcaaaaa aactatttgg
     2161 agtagtggtt ttgaatgcgg tgatcacttt atcagaattt attggaggga ttatgtcagg
     2221 gagtttgtct cttgtttcag atgctttcca taaccttagt gatacattgg caattatttt
     2281 ttcttacggg gcacaaaaaa tggcccgaaa agaagcaaat aaaaaacgta cgtatggcta
     2341 ccagcgttta gaaatattga gtgctttcat caactcattc atattgatca tattatcctt
     2401 gtttttaacc gttgaagcat tcaagcggtt cacttcacca gaaaagataa atagtcattt
     2461 gatgctaaca gtagcagtta ttggattgct cgcgaatttg ttctcagtat tattattacg
     2521 acaagaagca gatgaaagtt tgaatataaa atctagttat ttgcatttgt taagtgatac
     2581 actgtcttcg gtaagtgtga ttatcggagc aattttgatc cgttttttcg atatctactg
     2641 gatcgatcca gttattacac tgatcatttc gatatatata ttgatagaag cgataatggt
     2701 gatcaaaaaa gctgccgcta ttttgataca aagtgcacct gctatcgact atgaaaaaat
     2761 ggaacaagaa attgaagcaa tcgatggggt aaaggatgtt catcatgtcc atatttggca
     2821 gtatagcgaa aagataatta ttttcgacgg acatattgat tttgaagatc agctactaag
     2881 cgagattgaa aagatatatc ctcgtataat ttctttatta aaattaaaat atggtattac
     2941 acatgtgacg atccaagcag agacaaaggc aaaggatcaa aaaaaattaa tttttttaaa
     3001 taaagatgaa agttattgaa atatagagta tgataaataa gaatttttaa taaggtaggt
     3061 tggctcatga agcagtatcg acagttaaaa ggatatttag aagcaaaagg atattgggag
     3121 atactagtct atctcttttt tggtggtttg gctacgattg taaattttgt tagttttgct
     3181 ctcgcacgcc aatattttga tttaagtatg gccttatcca attctatttc gtggttttgt
     3241 tctgtattgt ttgcttttgt aacgaacaaa ctttgggtat tccattcaaa atcaccgaat
     3301 tttacacatg cacttattga atgcggtaag ttcttcttct accgtatcct ttcttacgga
     3361 ttggacatgg gggcaatggt attgttgatc aatgttatga acagcaatga gtatgtggcg
     3421 aaaatcatca ctcaaattat tgttatcctt gcgaactata ttttcagcaa gttgtttatt
     3481 tttaaagaaa ctgaggtttt tgaagaagaa gtaggtcaaa gtgaaaaaga cgactaggca
     3541 taacaattga agaatttcct tctctttgtt actctttgta atgtagacat tattaagaaa
     3601 acattgagga ggaatttttt tatgacttac acattaccag atctacctta tgcatatgac
     3661 gcattggaac cttatattga cgaagaaacg atgcatctgc atcatgataa gcatcacaat
     3721 acttatgtga cgaatttaaa ttcagcaatt gagaaatacc cagaattagg cgaaaaaaca
     3781 atagaagaat tattatctga tatggacgct attccaacag atatcaagac agctgtacgt
     3841 aacaatggtg gcggacatgc taaccattca tttttctggg aaatcatggc accaaatgct
     3901 ggtggcgaac ctacaggaga aataaaagaa gcgattaatg aagcttttgg tgatttttct
     3961 tcttttaaag aagaattcaa aaaagcagcc gctggacgat ttggttctgg atgggcttgg
     4021 cttgtaatgg aaaatggaaa attagctatt acctctactg caaatcaaga ttctccattg
     4081 atggaaggaa agacaccaat tctaggtttg gacgtttggg aacatgctta ttatttgaaa
     4141 tataaaaatg tccgaccaga ttatattgca gcattctgga atgttattaa ttgggatgaa
     4201 gtaaacaaac gtttcgaagc agcaaaataa ccatttcctc cgtcatatca gtcttaaaaa
     4261 taatttggaa aataaaagag cgaaagcttg ctagcgtttt ctaaattggg tataatgttc
     4321 ttgcaaggtt acttgcaaga acttttttgg aggatataca tttatggaac acggaacggt
     4381 taaatggttc aacaatgaaa aaggatttgg gtttattaca gtagacggcg gagatgacgt
     4441 atttgttcat ttctcagcaa ttcaaggaga cggcttcaag agcctagaag aaggtcaaga
     4501 agtagaattc acgattgttg aaggtgcacg cggacctcaa gctgctgaag tgactaaatt
     4561 ataataattt gttgaataaa gacacgactt cttagagagt cgtgtctttt tgtctattta
     4621 tgaagttttt agattttttg gctaagtaaa tgcatttgtt cacttgacat gtcctaacta
     4681 attatcaagc aaggaacatt ttcttcgtta gtaatggatt ttaaagaaat ttcagttttt
     4741 ggatgttgaa taaaagacca tttgtgaaac cactagtata catactcatt accatgtcaa
     4801 ttctattact ttaaaagaaa atcgttttac tttgaagagg agcatatcta tccattaaaa
     4861 gatgatggta atgcaatcac tagttcgagt cattttaaga gccatgcagt gagctggaaa
     4921 taaaacgaat agaagatggg tttaacaaaa ggaccagtca taggaagaaa ttgtcgtgaa
     4981 atcgaatgat aatctgaacg gtaggcaaag actgttaaaa agatgggaaa ggtatgtgcg
     5041 atcattttac tgcacgatta tttggaaaat accttgttgt tcgctttttc tggaaaaaat
     5101 acataaaaaa agataaaacg attgcgaact gcgtgtattc ggtctttcga tctttttcac
     5161 ggtatggtat gatagttaag aattttgaga aattttaaaa aataataatc gttacacaaa
     5221 aatgtttttt aaagttttct tatgattaaa aagtgaattt gctgattgaa cagttatatg
     5281 agacaatatt ttagaaaatg tgaaacagag gtgtattatg aagcgatggc aaaaagttct
     5341 attaaccttg ttaggggtat tcgttttgct gattgccgga ttatcagcat acggaatcag
     5401 atttatggga gaagcgagcc aaacagtaaa caaaatttct aaaaactcta atcgaatatc
     5461 ctccaaacgt tctgagagag taagcattga tgacaaagaa cctttttcgg ttttgctatt
     5521 aggtttagat accggtggat taggaagaac ggaacaagga cgttctgata cgatgatggt
     5581 agtaacagtt aatccgaaac aaaaaaaatc aacgatcatc agtttggacc gagatattta
     5641 tacgaatatc gtaggttatg gaacggtaga taaattaaat catgcctacg catttggtgg
     5701 tgtcgaaatg gccatggatt caattgagca actgctcgat attcctattg atcattatgt
     5761 aacgatcaat ttggatggca tggaagattt gatcaatgcg gtcggtggca tcaaagtgaa
     5821 taataaaatt gactttactt tggatggtgt ccatgtacca gtaggtgaac aaacattgaa
     5881 tggagaaaaa ggtttagcct attctcgaat gcgacacgaa gatccagaag gcgatatcgg
     5941 tcgacaacgt cgtcagcggg aagtagtgac gaaaatcgtc aataaagttt taagtttaga
     6001 tggcgtaagc aattatagaa aaattttaaa agcagttgag aacaatgtga cgacggactt
     6061 agattgggat gacatgcttg atgtagcgac gaactacacc ccggcgtttg aaacaatcaa
     6121 acaagaccag ttgcaaggcg aaggacaaat gatcaatgat atctactatc aaattttagg
     6181 aaaaaatgat cttctttcga tccaaaatga actgaagaaa caattaaata taaaaacaag
     6241 cgatacatta cctaatttga aaaatgagaa tgcttcaatg atgttctttg atgattcgga
     6301 agatggcgat tcgacaaacg gtgatggatc gaatagtgaa gggtacaccg attattcttc
     6361 ctattcagaa aacacatatg atcagtacca agatccgaat acctattatc agcaaacaga
     6421 agatacaagt cagtactata caccaagtca agatactcaa agttatgatc cttacgctgg
     6481 acaggaacaa aatggttatg gttatgacca gaataatgag tatggaacaa caagtgggta
     6541 ctagaaaaaa atagtttggt taataaagag tgccacctaa attagacaaa aaa
//
LOCUS       DAZKSU010000107         6328 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000107 DAZKSU010000000
VERSION     DAZKSU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.110"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..1228)
                     /locus_tag="ACG8Z3_002572"
     CDS             complement(98..1228)
                     /locus_tag="ACG8Z3_002572"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0824826.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ACG8Z3_002573"
     CDS             complement(1877..3079)
                     /locus_tag="ACG8Z3_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0824827.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ACG8Z3_002574"
     CDS             complement(3139..3342)
                     /locus_tag="ACG8Z3_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0824828.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4551)
                     /locus_tag="ACG8Z3_002575"
     CDS             complement(3484..4551)
                     /locus_tag="ACG8Z3_002575"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0824829.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4609..4716)
                     /locus_tag="ACG8Z3_002576"
     CDS             complement(4609..4716)
                     /locus_tag="ACG8Z3_002576"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824830.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4879..5691
                     /locus_tag="ACG8Z3_002577"
     CDS             4879..5691
                     /locus_tag="ACG8Z3_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824831.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6065..6256)
                     /locus_tag="ACG8Z3_002578"
     CDS             complement(6065..6256)
                     /locus_tag="ACG8Z3_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824832.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1884 a         1300 c          997 g         2147 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaaca
//
LOCUS       DAZKSU010000108         6173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000108 DAZKSU010000000
VERSION     DAZKSU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.111"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACG8Z3_002579"
     CDS             725..811
                     /locus_tag="ACG8Z3_002579"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0824833.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACG8Z3_002580"
     CDS             complement(932..1621)
                     /locus_tag="ACG8Z3_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824834.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACG8Z3_002581"
     CDS             complement(1866..2780)
                     /locus_tag="ACG8Z3_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0824835.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACG8Z3_002582"
     CDS             complement(2762..3142)
                     /locus_tag="ACG8Z3_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0824836.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACG8Z3_002583"
     CDS             complement(3592..3879)
                     /locus_tag="ACG8Z3_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0824837.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACG8Z3_002584"
     CDS             complement(3899..4123)
                     /locus_tag="ACG8Z3_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0824838.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACG8Z3_002585"
     CDS             complement(4578..4748)
                     /locus_tag="ACG8Z3_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824839.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACG8Z3_002586"
     CDS             complement(4763..5296)
                     /locus_tag="ACG8Z3_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0824840.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACG8Z3_002587"
     CDS             complement(5317..>6173)
                     /locus_tag="ACG8Z3_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0824841.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAZKSU010000109         6111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000109 DAZKSU010000000
VERSION     DAZKSU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.112"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..634)
                     /locus_tag="ACG8Z3_002588"
     CDS             complement(38..634)
                     /locus_tag="ACG8Z3_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0824842.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            984..1328
                     /locus_tag="ACG8Z3_002589"
     CDS             984..1328
                     /locus_tag="ACG8Z3_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0824843.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1329..1748
                     /locus_tag="ACG8Z3_002590"
     CDS             1329..1748
                     /locus_tag="ACG8Z3_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFQ0824844.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1840..2190)
                     /locus_tag="ACG8Z3_002591"
     CDS             complement(1840..2190)
                     /locus_tag="ACG8Z3_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824845.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2156..3487)
                     /locus_tag="ACG8Z3_002592"
     CDS             complement(2156..3487)
                     /locus_tag="ACG8Z3_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0824846.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3742..4308
                     /locus_tag="ACG8Z3_002593"
     CDS             3742..4308
                     /locus_tag="ACG8Z3_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0824847.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4453..4540
                     /locus_tag="ACG8Z3_002594"
     tRNA            4453..4540
                     /locus_tag="ACG8Z3_002594"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4485..4487,aa:Ser,seq:cga)
     gene            4876..5850
                     /gene="bsh"
                     /locus_tag="ACG8Z3_002595"
     CDS             4876..5850
                     /gene="bsh"
                     /locus_tag="ACG8Z3_002595"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0824848.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         2219 a         1027 c         1130 g         1735 t
ORIGIN      
        1 acattgtcaa tcaatgacta attttacaaa ataaatatta tccaagaaga ccacttagat
       61 attgacctcc ataaatcaag gccatactat ataagaaaat cgacagtggt ttagcaatca
      121 aaatgattag tgtatatcgt ttcactgaca tgtttgtcaa tccagccatc aaacataacg
      181 catcgtcagg agcaactgga agaaaaatag ctagagcaaa cagcttctca aaacggtttt
      241 gattatccag ccagccaata tatttgttat atgtcttatc gctaatcatg gaaagtatga
      301 atggctttcc ataacgacgc cctagcagaa aaatgataat cgaaccaatg cagattccaa
      361 tatagttata gataaatcca gcataagggc caaatatcag gacaccagct gcagtactga
      421 tccctccagg aatgattgga ataactactt gcaggatttg gatgaagaca aaaataatcg
      481 gtcctaaaat gatggaatca cctactaatc ctcttaacgc attcaaatct ttgaaaacac
      541 ctaaattaat aaaataaatc gtcagcgcga ttgataggcc taatccgata atggatatga
      601 aatttatcag ttttcgagag gcagcaatat tcattattca tcaactcctt agaggttctt
      661 tgttaactat actttactag aaaagcaact aactgcgcca ttttttgata tgacagaaaa
      721 caaacaattt tgtaagttca tcataagtat aaaagattga tctcaaaaaa acatccaact
      781 aaagacttat ttaataactt gtttttttta aaataaaaaa agcgttagca actatgctta
      841 aaaagcatat cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg
      901 aaatgcgaag aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca
      961 gtcgtgaagg gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag
     1021 gagattttgc accaaaattt gcagaattaa atgatgatgt tttatttgga caagtatggt
     1081 ccagagaaga attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag
     1141 gaggaaattt tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta
     1201 gtgaagaaat agcagaaatc attacccatt tagcttttta tgtagaatgg ccaaaagctt
     1261 ggtcagcttt tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag
     1321 aggcgtaaat gatgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg
     1381 aaaaaaacga agcttacgct caagtattta ccgggcaaag ctatctgaat atgttagtga
     1441 atgatcccga agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc
     1501 actggcatgt ccatacagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt
     1561 atcaggaaga aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca
     1621 aagggattaa acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca
     1681 ctgctggtaa ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa
     1741 atcaataatg tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc
     1801 tgcttttttg attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc
     1861 acgtcgaaag atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat
     1921 caaactacct aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg
     1981 atagtagatt tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa
     2041 atcttctggc tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa
     2101 ttctactcca atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc
     2161 gatgattctt tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa
     2221 agttatctag tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat
     2281 atagatttag tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagcacac
     2341 cagctattac accgtaaaaa ctgccgtaaa aaccgcttat gggcgcaaga cctgtcccaa
     2401 acacagctgc aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag
     2461 caataaatat tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga
     2521 aacccaccgc agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca
     2581 aaacataaga agacagcatc agaccaacta agcccatgtt gatcaaagta gcaccaatac
     2641 cagaattagc aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc
     2701 ccgaaagccg aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa
     2761 aaataatcca aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg
     2821 aatagctgaa taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc
     2881 caatattata aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa
     2941 gaagtatacc aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa
     3001 aacgcatacc aaaagtgata taactaacga ccggtgataa tacgctacca aacagtccga
     3061 tcatgatgta ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa
     3121 tgattgagat agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa
     3181 tcgaagcaaa taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa
     3241 gtaaggtcaa aatccctaca tttatgaatg cgcttccgaa aacacctaaa gcaaaataat
     3301 ctgtcaaaag attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag
     3361 gagtattaga aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat
     3421 actttctttt ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt
     3481 tttccattac acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg
     3541 acaactatca tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt
     3601 tttttaattt tgcaaaaaaa agttaaagaa gactgaaaaa aatttaaaat ataacgtttt
     3661 gagtaaaatg tttgcaaaaa atgctaaaaa tagggtacaa taaaacaaca tatactgcag
     3721 taaagaagtg ggggagtttt aatgagaaga aaagtctata ccaaggatca aattctaaaa
     3781 gctgcttatg atgttgttgc aaaggaaggg tttaaaggat ttactgcgag aaatattgct
     3841 aaaaagatgg ggatttcgac acagccgatt tatctggaat tcaaaaacat ggaagattta
     3901 aaaaatacgt tgctggattc tatctttgaa caactaaaag aagatgtttt ccctattgaa
     3961 cgtactggtg ataaaatcat tgatctcggt ttgaactata tctactttgc aaaaaagcat
     4021 catagtcttt acatggcatt gtacattgat gaacatgggg acggaaagaa aatgcatgat
     4081 ttttcataca attatttccg tgaaatagtt aaagaagatg aaagatacaa tcaattatcg
     4141 gatgaatatg tcgatgctct gcatactggt acatgggtag cagtgactgg cttagcgtca
     4201 ttgatgtcat caaacatcat gcatccaaca gaagaacaaa tcattgcact gatcaaacgg
     4261 acaattgact cgattttagc attagataat cctgaaataa tcagatagtg ttctatcagt
     4321 atgaatttga tcggtaagtc cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa
     4381 agcgatttct gttatttatc tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa
     4441 aatagtactt acggaaggtt ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc
     4501 ggctaatacc ggcgcgcagg ttcaaatcct gtaccttcct tatatcaacg attagaggtt
     4561 gtgaagaagc tgtcctgcat caagtaataa gaacggccaa acaaaaatag cttttcctat
     4621 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     4681 ataaggggct gtgacaagac ttttgtcaca gccccttatt gttgtacttt taacctacaa
     4741 tattttttga ttccccctga gtactatttt ttataaatgc tagtaaatca atcttctttg
     4801 gccttttttt tgataaaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt
     4861 ttaggaggga aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga
     4921 aggaattttg attatgagat gtcttataat gaagtagtca ctattactcc aagaaattat
     4981 cgtttcgatt ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca
     5041 gctggggtat ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg
     5101 gcaggactga attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac
     5161 gttgcaccat ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct
     5221 aaagaattat tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg
     5281 tcacctttac actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa
     5341 gatggacttc atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat
     5401 tatcaattat ttaatctgaa caattatcgc tcgctatcaa atggaacacc agaaaatcat
     5461 ttttcaaacc agatcagctt gaatgtttat agtcgtggga tgggaggatt gggcttacct
     5521 ggagatctct cttctgtttc tcgatttgta aaagccacat ttactaaaat gaatgctgca
     5581 tcaggtgatt cagaatctga aagtatcagt caattttttc atatactagg ctcagtcgag
     5641 caacaaaaag gagtatgcga tactggagaa ggaaaatacg aatatacgat ttattcttct
     5701 tgctgtaacg ttgataaagg gatttattat tatcgtacgt atgaagacag tcaaattact
     5761 gctgtaaata taaataaaga agacttggat cgtcatgaat tggtcagtta tccagtcata
     5821 aaagagcaac agataaacta tgtcaattaa ttaatgaata gatccgtttg gtatcatatt
     5881 ttctgataca gtcataatta gacacgatct ttaggtcgtg tctttttttg ctgttttttt
     5941 tggaaatagc atccactaat atttttttaa cacgaagaga ataaggtagc ctcaaaaaag
     6001 aaaacatggt aaattagata ttgggacttt attttctgta aaaacagaaa ctttcatctt
     6061 ttgttttcac gtgacgaaat tgtaagagag aaaatattcg tccgctattt a
//
LOCUS       DAZKSU010000110         6068 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000110 DAZKSU010000000
VERSION     DAZKSU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.113"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            832..1566
                     /locus_tag="ACG8Z3_002596"
     CDS             832..1566
                     /locus_tag="ACG8Z3_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0824849.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="ACG8Z3_002597"
     CDS             1582..2751
                     /locus_tag="ACG8Z3_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0824850.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8Z3_002598"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="ACG8Z3_002598"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0824851.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="ACG8Z3_002599"
     CDS             3841..4767
                     /locus_tag="ACG8Z3_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0824852.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="ACG8Z3_002600"
     CDS             4786..6036
                     /locus_tag="ACG8Z3_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0824853.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DAZKSU010000111         6058 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000111 DAZKSU010000000
VERSION     DAZKSU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.114"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            194..2101
                     /locus_tag="ACG8Z3_002601"
     CDS             194..2101
                     /locus_tag="ACG8Z3_002601"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0824854.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2642..5920)
                     /locus_tag="ACG8Z3_002602"
     CDS             complement(2642..5920)
                     /locus_tag="ACG8Z3_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0824855.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         1753 a         1237 c         1142 g         1926 t
ORIGIN      
        1 ctactttgaa atataaaggt ttatccacta cttgattcag ttgggatcgc ctcctttttt
       61 tcattataag tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt
      121 ttatgttcta tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag
      181 gagcgatcga tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga
      241 agaatggcaa aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga
      301 tttagttgac gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc
      361 agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga
      421 ttatgaccga agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga
      481 atcattgcag tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt
      541 accattgcca tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt
      601 ctttgcttat ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc
      661 caacggtgca caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc
      721 cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt
      781 gattgctcct catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc
      841 gcaatctttt gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac
      901 gggtttgatt gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca
      961 tttgaatgac atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa
     1021 aacagtacgt tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta
     1081 ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct
     1141 tgcctctgga caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga
     1201 agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg
     1261 tctagaagaa aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa
     1321 taacttaggt atccaagtag gctaccccga taagatccca gcattattcg accagttcaa
     1381 aacagtacct gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact
     1441 cacattaaaa gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat
     1501 gagtgctgac acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc
     1561 agcgatcctt caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg
     1621 aatcggagct gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt
     1681 tgacgaatat ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa
     1741 caaagcacaa gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa
     1801 cggaaaatta accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga
     1861 agcagcaaaa acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat
     1921 ttggcgtaca aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc
     1981 ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt
     2041 tggtatcacg gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta
     2101 agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt
     2161 ctcaaatttt tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt
     2221 attagaggtt gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg
     2281 ctttccacat ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt
     2341 tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat
     2401 agttcctcat attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt
     2461 ttagtggaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa
     2521 gcagcaagga gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca
     2581 tttccttcta tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca
     2641 cttatttttt tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag
     2701 ctaatgctag caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt
     2761 tttcactttc ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg
     2821 gtgattcatt tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag
     2881 tttctcgcac agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt
     2941 ccccatcaaa tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg
     3001 ctgaaaaaat agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga
     3061 tcttttctgg gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag
     3121 ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca
     3181 caccatttgc ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg
     3241 gttcttgtac gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa
     3301 tagcacctac agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg
     3361 gtttaatctg catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct
     3421 gctgaccttc tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc
     3481 caagagtatt ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga
     3541 tttgatcttc gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc
     3601 ggaaagataa gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag
     3661 ccacattata tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc
     3721 tattggaatc gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag
     3781 ggcccgttaa agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc
     3841 ctggtaccca gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac
     3901 ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac
     3961 cgtcaaatac atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa
     4021 atcctggatc ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg
     4081 cttcgaaagg atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag
     4141 catgaatctc ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg
     4201 cactatttgt aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat
     4261 ttccagttcc tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca
     4321 actgaatcgg gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga
     4381 aggaataagt tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt
     4441 gtgtcagaga taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta
     4501 cataaactgt cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt
     4561 cataactagt catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga
     4621 aagtaaattc gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag
     4681 tgatagccaa gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca
     4741 ataatccgtc agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac
     4801 tagaggtcac agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat
     4861 ctgtcgcagg atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg
     4921 ctgcagcaaa tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat
     4981 acatgatgtc agaagccatt tcttctcctg tttcaggatc gtaataatat gcctctaaac
     5041 cagtattcgt ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca
     5101 gattcccatg accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg
     5161 ctgaagtctc taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg
     5221 gatcttcaga tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt
     5281 tgctgccgct cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac
     5341 tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta
     5401 ctgcgtctct taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta
     5461 tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg
     5521 tcattccatc tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca
     5581 cgccgtcttc taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa
     5641 cccctttcca ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt
     5701 ctttcatcat aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtattt
     5761 cctccattgt tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt
     5821 gttcgttagc agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat
     5881 tcatcattag cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa
     5941 attagaaagc gtttacttaa agtattacct aatttatttt tactgtcaat aaaaaacaaa
     6001 attataataa gtgtatttta taaaaagaaa aaatataact taagttgttg ataataaa
//
LOCUS       DAZKSU010000112         6058 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000112 DAZKSU010000000
VERSION     DAZKSU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.115"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            292..2523
                     /gene="pflB"
                     /locus_tag="ACG8Z3_002603"
     CDS             292..2523
                     /gene="pflB"
                     /locus_tag="ACG8Z3_002603"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0824856.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            2767..3528
                     /gene="pflA"
                     /locus_tag="ACG8Z3_002604"
     CDS             2767..3528
                     /gene="pflA"
                     /locus_tag="ACG8Z3_002604"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0824857.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            3613..4539
                     /locus_tag="ACG8Z3_002605"
     CDS             3613..4539
                     /locus_tag="ACG8Z3_002605"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0824858.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            4721..5614
                     /locus_tag="ACG8Z3_002606"
     CDS             4721..5614
                     /locus_tag="ACG8Z3_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0824859.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            5748..5873
                     /locus_tag="ACG8Z3_002607"
     CDS             5748..5873
                     /locus_tag="ACG8Z3_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0824860.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
BASE COUNT         2143 a         1022 c         1195 g         1698 t
ORIGIN      
        1 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
       61 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
      121 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
      181 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
      241 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
      301 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
      361 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
      421 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
      481 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
      541 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
      601 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
      661 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
      721 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
      781 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
      841 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
      901 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
      961 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
     1021 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
     1081 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
     1141 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
     1201 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
     1261 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
     1321 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
     1381 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
     1441 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
     1501 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
     1561 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
     1621 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
     1681 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
     1741 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
     1801 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
     1861 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
     1921 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
     1981 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
     2041 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
     2101 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
     2161 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
     2221 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
     2281 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
     2341 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
     2401 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
     2461 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
     2521 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
     2581 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
     2641 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
     2701 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
     2761 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
     2821 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
     2881 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
     2941 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
     3001 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
     3061 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
     3121 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
     3181 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
     3241 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
     3301 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
     3361 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
     3421 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
     3481 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
     3541 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
     3601 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
     3661 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
     3721 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
     3781 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
     3841 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
     3901 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
     3961 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
     4021 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
     4081 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
     4141 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
     4201 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
     4261 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
     4321 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
     4381 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
     4441 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
     4501 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
     4561 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
     4621 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
     4681 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
     4741 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
     4801 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
     4861 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
     4921 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
     4981 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
     5041 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
     5101 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
     5161 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
     5221 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
     5281 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
     5341 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
     5401 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
     5461 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
     5521 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
     5581 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
     5641 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
     5701 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
     5761 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
     5821 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
     5881 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
     5941 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
     6001 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaat
//
LOCUS       DAZKSU010000113         6000 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000113 DAZKSU010000000
VERSION     DAZKSU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.116"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..934)
                     /locus_tag="ACG8Z3_002608"
     CDS             complement(65..934)
                     /locus_tag="ACG8Z3_002608"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0824861.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1037..1249
                     /locus_tag="ACG8Z3_002609"
     CDS             1037..1249
                     /locus_tag="ACG8Z3_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824862.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            complement(1226..2836)
                     /locus_tag="ACG8Z3_002610"
     CDS             complement(1226..2836)
                     /locus_tag="ACG8Z3_002610"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0824863.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(3058..3303)
                     /locus_tag="ACG8Z3_002611"
     CDS             complement(3058..3303)
                     /locus_tag="ACG8Z3_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824864.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFGTHKGLKK"
     gene            complement(4029..5369)
                     /gene="glnA"
                     /locus_tag="ACG8Z3_002612"
     CDS             complement(4029..5369)
                     /gene="glnA"
                     /locus_tag="ACG8Z3_002612"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0824865.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(5397..5789)
                     /locus_tag="ACG8Z3_002613"
     CDS             complement(5397..5789)
                     /locus_tag="ACG8Z3_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0824866.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1760 a         1281 c          988 g         1971 t
ORIGIN      
        1 agaatggaga gcgacttcaa aagtcgttct ccattcctat atatcgtatc tgttatgtgg
       61 atgcttacgc tttgttagct gaaccgaacc agtcgatatg agatttagca tcttcaacga
      121 ttgctgcttt acctggagct aataatttac gagggtcgaa tcctttacct tctttatctt
      181 taccagcttc gatatattta cgagtagctg cagcaaatga taattggaat tcagtgttaa
      241 catttacttt tgagataccc attgagatag ctttttcgat ttgatcttga ggaatacctg
      301 atccgccatg taatactaaa ggcatgtctg gacctacagc atcagcaatt gcttgtaagt
      361 ggtcaaaagc taatcctttc cagttttctg gatattgacc atggatgtta ccgataccgc
      421 aagctaagta gtcgatacct gtagcaacca tttgtttgca ttcttcaata tcagctaatt
      481 cgccgttacc gatgattccg tcttcttctc cgccgattga accaacttca cattcaactg
      541 agataccttt tgcatgtgct ttttcaacaa cgtctttagc taatttcaag ttttcttcaa
      601 atggtagatg tgaaccatcg aacattactg aagtatagcc taattcgata cattccaaag
      661 cagcttcgta atcaccatgg tctaagtgga tagcaactgg aacagtgatg ttcattgctt
      721 caactaaatc agtgattaaa tctttacaga ttttgtaacc acccatgtat ttagctgcac
      781 ccattgatgt ttggataagt actggtgctt gttttgcttc agccgcttca aggattgctt
      841 gagtccattc caagttgttt gtgttgaatc cgccgacagc gtaaccgcct ttacgagcag
      901 cttttaaaaa ttcagctcct gatactactg gcataataaa attcctccta agttttgtaa
      961 aactaaattt gttaagacaa ttcttaacca acgaccctat tttaccaaac atttcggtag
     1021 ttttctacta aaatcaatga aaatgaaaat tttcaagaag ttttttgcaa aaaataaaat
     1081 atccaatagc gctttaatcg aaaaaagtct tttgctttct atcttttttc ctacaaataa
     1141 taacaaatca gaaaaagaga aaagtgacaa gaaaattgct tatccccttc tcttgctact
     1201 tttctctttc tatcaagttc aaaaattatt ttgtttcgtg atctgctaat gatgctccga
     1261 caaatgcttt gattaatttt tgcgggcgat ttggacgaga aatcaattct gggtggaatt
     1321 ggcatcccac aaagaatttt ttctctggca gctctacgat ttctactaaa cggttatctg
     1381 gtgagacacc tgaaaagact agcccttgtt cttcaaacaa ttgacgatat ttgttattga
     1441 actcatagcg gtgacggtga cgttcttgca caacatcggc tccatcatat gcttcagccg
     1501 tcttcgttcc ttttttgatt ttacatgggt ataatccaag gcgtaaagtt ccacccaaat
     1561 tctcgatgtt ttcctgatca gccattaaat caataatatt gtttgctgta tctggattcg
     1621 tttcagctga tgacgcatct tctaatccaa cgacgttacg tgcaaattcg acacaagcca
     1681 tctgcatacc aagacaaata cctaaaaacg gaacgtcatt ttcgcgagca taacgaatcg
     1741 cttcgatttt cccttctact ccgcgatcac caaatccacc aggaaccaag attccatctg
     1801 cgtctttcaa acgttcttct acattttctc tcgttaattc ttgtgctttg atccaatcga
     1861 tctcgatgtc tgaattatag gcgaaaccag aatgtttcaa tgcttcaact actgagatat
     1921 atgcatctgg taactcgaca tatttaccga caagcgcaat ccgtactttt ttcttcaagt
     1981 tcaagacacg ttcttctaat tctttccatt ctgtcatgtc tgcttctggt gtatctagtt
     2041 ttaaatggtc gcaaacaatt tgatccattc cttgtgcttg taaaactaat gggatggagt
     2101 aaagtgtatc tacatctggc gactcgataa ctgcttctgg ttcgacatca caaaattgtg
     2161 ccaatttgtt tttcacgctt tgagaaacag gttgctctgt acgtaccacc aaaatattcg
     2221 gttggatacc taaaccacgt agctctttta cactgtgctg cgttggttta gttttcattt
     2281 ctccagcagc tttcaagtaa ggaatcaatg ttgtatggat atacataaca ttatctgtac
     2341 cgacatctgc tttcatttga cgaagtgctt caaggaatgg caaagattct atatccccta
     2401 ctgttccccc tacttctgta atgatcacat ctgaatcagt cattttagct gctcgcatga
     2461 ttttttcttt gatctcattt gtaatatgag gaataacttg aacagttgct cccaagtatt
     2521 cgcccttacg ttctttacgc aaaacttcag aatagatttt tcctgtcgtc acgttggagt
     2581 atttgttcaa attgatgtca ataaaacgtt cgtagtggcc cagatccaaa tctgtttcag
     2641 caccatcatc tgttacgaat acttctccgt gctgatatgg gctcatcgtc cccggatcca
     2701 cgttgatgta tggatcaaat ttttggatag ttacttttaa gccgcggttt ttcaataagc
     2761 gtcctaaaga agctgcaaca atcccttttc cgattgatga aacgacaccg ccggtcacaa
     2821 aaatatattt tgtcataagt tcaaaaactc ctttttaatt tatgtgcagg aaaagtctca
     2881 gacaataaac aaagctccct attcttcaat aagaataggg agccgaaatt tctgtaaact
     2941 tctgaccctc taaaagggtg cccaaggtgt ataatacaag ctgactaagg gaaagtcaag
     3001 tactcaaatg aatactatcc gcttacaatt ttttcttttt cgatatcctt tgagaaatta
     3061 tttcttaagt cctttatgag tgccaaaaaa atttaaccca cataataagc ttgatataaa
     3121 catagctaac gttaaaccta agcaaatcag aaaatcgtta ctgaaaaatt ttaagtgaaa
     3181 taatagataa aaaaataaaa caaacattat gatagataat atcccaccta acaaagaact
     3241 actttttcct acttgcagca acagtttata tagcttatcg tagaaacaat ctttttcttt
     3301 cacccttatt tacctccttt acgtaaaaaa cgtagtccac agttctcgga tttttggcat
     3361 atcttttcta ttaaataata tcaaaaacaa aacaattcca ctaatttttt caaagatgac
     3421 ctttttttca acaaaagtgc cttgtaggga ttcattgaag gtaacgaatc acataagtaa
     3481 aaaataagga gatgcgacaa aagtcgtgcc tccttatttc taaataaacg gtgttctcct
     3541 gccaactcct cggtcacaag tcacaatatc catcaaacat gagaagctgt ttttgaaatt
     3601 atttcttgtg attcggagtt aaatggtagg ctcacaacct ctggataaac ggtattcaaa
     3661 agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca ttttcacaaa
     3721 atttttctta atactcgaaa ctaaacgtct ttttcaaaac ctcttggtaa acggtgttca
     3781 aaagcttgca tctgcggtaa taagttcagc caattcaaaa acaagaaaag ctgtttttgc
     3841 ttggctattc ttattacttg ttgcagggca gctttttcac aacctctcca ctaaacggtg
     3901 ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttt
     3961 ccgttagttt ctctaatact caggtcaaaa cgctgatttc acagcctcta attcacggta
     4021 ttcctgtttt aataaagttc tagatattgt tctctttccc actcggaaac agtttgtctg
     4081 aatgccgccc attcgagacg ttttgcttca acaaaatttg agaagatatg ttgtcctaat
     4141 gcgtcaatca ttacattatc cttacgcaat tctttgatag cattatggat agttgaaggt
     4201 aagtcctgga tatgtgcagc ttctctttct tcctcatcca ttacataaat gttacggtca
     4261 acagctgctg gcggtgtgat ctgattacgg ataccgtcta gtcctgcttg caacaatact
     4321 gccattgcca agtatgggtt tgcagatgga tcaactgacc gcaattccaa acgtgtggat
     4381 aaaccgcgag attctggtac acgtactaat ggcgaacgat tacggccact ccatgcgaca
     4441 taaacaggcg cttcataacc tggaacgaga cgtttataag aattgactgt tggattgcaa
     4501 acggctgtgt aggcacgagc atgtttcagc aaaccaccta agaaatgata agctgtttca
     4561 cttagttcca attcaccgtc tttatcatag aaaacatttc cttcatcatt gaacaaagac
     4621 atattgcaat gcataccaga accattgatc ccaaataacg gtttcggcat gaaagtcgcg
     4681 tgaagtccat gcttgcgtgc aatcgttttg acaacgagct tgaaggtttg gatattatca
     4741 caagcatcta ccacatctgc atatttaaag tcaatttcat gctgtcctgg cgctacttca
     4801 tgatgtgaag cttccacttc aaagcctaga ctttctaatt ccaacacgat gtcacggcgg
     4861 caattttcac ctaaatctgt tggtgcaaaa tcaaaataac cacctttatc attcaaggtc
     4921 gttgtgatcc caccgtcttc gtctaattta aataagaaga attctggttc aggtcctaaa
     4981 ttgaaagagg taaaaccaag gtcgcgcata tctttcaatg ctcgtttcaa attcccgcga
     5041 ggatcgccgg caaatggtgt cccatccgga ttatagatat cacaaattaa acgagcaact
     5101 tttccatgag cactttccca cgggaagatc atccaagtgg agagatctgg atacaaatac
     5161 atatcgcttt cttcaattcg aacaaatcct tcaatcgaag aaccgtcaaa catcatttta
     5221 ttttctaata ctttgtcgat ctggctcact ggaacttcta catttttgat cgtacctaag
     5281 atgtctgtga acatcaatcg taaaaatcgt acgttttctt cctcaatgat tcgtttgatt
     5341 tcttcacctg ttatctgttt ctttaccatg gtatccctct tcctttttta atttattcag
     5401 tcaaagaatt gtctttgcaa tccttgcgac tgataatgac ctgttttgct taacccacct
     5461 tgcgccaaaa tctcatcacg aaaaatttgt cgaacgtcat gatctgtcaa cggcttcttg
     5521 ttttttgctt cttgtttttt cgcctgtgcc atttcgtata cacgtttgat cccagccata
     5581 ttcaatccct ctgaaagata gtctttaatc tcaagcaaca tatcaatatc attcagcgaa
     5641 tacatacgtc ggtttccttc gcttcgttcc ggatgaacta aatcttgttc ttcataatag
     5701 cgaatttgcc gagcagacaa atcagtcagc ttcataactg taccaattgg aaatacggac
     5761 atagatcggc gcaattcctt ttctcccatc agacgtcccc tttcgtttta taagttggct
     5821 aaagtgtacc aattaaaaaa aatattgtca actgcatatg ttaggttttc taacatgaga
     5881 tctctatttt ttataaaata acaaaaaaat aagaacacaa aaaaggtatc tagttgattt
     5941 tcgtaccaga taccttttat tttttattat actgtactat cctttattag tgtttttatt
//
LOCUS       DAZKSU010000114         5962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000114 DAZKSU010000000
VERSION     DAZKSU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.117"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..450)
                     /locus_tag="ACG8Z3_002614"
     CDS             complement(<1..450)
                     /locus_tag="ACG8Z3_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0824867.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="ACG8Z3_002615"
     CDS             674..1465
                     /locus_tag="ACG8Z3_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0824868.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="ACG8Z3_002616"
     CDS             1431..2384
                     /locus_tag="ACG8Z3_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFQ0824869.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="ACG8Z3_002617"
     CDS             complement(2782..3756)
                     /locus_tag="ACG8Z3_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0824870.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="ACG8Z3_002618"
     CDS             3871..4305
                     /locus_tag="ACG8Z3_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0824871.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="ACG8Z3_002619"
     CDS             complement(4430..5452)
                     /locus_tag="ACG8Z3_002619"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFQ0824872.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>5962
                     /locus_tag="ACG8Z3_002620"
     CDS             5844..>5962
                     /locus_tag="ACG8Z3_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HFQ0824873.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIET"
BASE COUNT         1873 a         1124 c         1027 g         1938 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag ac
//
LOCUS       DAZKSU010000115         5954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000115 DAZKSU010000000
VERSION     DAZKSU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.118"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..813
                     /locus_tag="ACG8Z3_002621"
     CDS             <1..813
                     /locus_tag="ACG8Z3_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0824874.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            786..1229
                     /locus_tag="ACG8Z3_002622"
     CDS             786..1229
                     /locus_tag="ACG8Z3_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824875.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2399..3367
                     /locus_tag="ACG8Z3_002623"
     CDS             2399..3367
                     /locus_tag="ACG8Z3_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0824876.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3384..3908
                     /locus_tag="ACG8Z3_002624"
     CDS             3384..3908
                     /locus_tag="ACG8Z3_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0824877.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            4284..4565
                     /locus_tag="ACG8Z3_002625"
     CDS             4284..4565
                     /locus_tag="ACG8Z3_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824878.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4567..4983
                     /locus_tag="ACG8Z3_002626"
     CDS             4567..4983
                     /locus_tag="ACG8Z3_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824879.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            5613..>5954
                     /locus_tag="ACG8Z3_002627"
     CDS             5613..>5954
                     /locus_tag="ACG8Z3_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HFQ0824880.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2258 a         1018 c          965 g         1713 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     1441 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
     1501 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
     1561 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
     1621 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
     1681 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
     1741 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
     1801 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
     1861 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
     1921 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
     1981 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
     2041 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
     2101 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
     2161 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
     2221 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
     2281 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
     2341 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     2401 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     2461 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     2521 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     2581 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     2641 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     2701 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     2761 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     2821 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     2881 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     2941 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     3001 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     3061 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     3121 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     3181 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     3241 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     3301 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     3361 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     3421 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     3481 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     3541 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     3601 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     3661 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     3721 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     3781 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     3841 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     3901 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     3961 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     4021 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     4081 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     4141 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     4201 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     4261 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     4321 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     4381 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     4441 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     4501 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     4561 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     4621 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     4681 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     4741 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     4801 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     4861 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     4921 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     4981 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     5041 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     5101 tcttcttttt gtattttggg gcgctacccc aatccccaag tatcggttat ttaatagcgc
     5161 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     5221 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     5281 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc tttaaaaaat gacagataaa
     5341 aatgcacaaa gaactaaaaa agagaaatcg cgtaaaatca agcattagaa gctagtaact
     5401 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     5461 ggtctagtcg acattatgtt aactagctcg ataaataacg attatgttcc caaaacaata
     5521 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt atataattat
     5581 aggtgtatac cacttaaatt taacacaaag acgtgattat tttgaaaccc aaaaagaaaa
     5641 tttatctagg tctcctcttt tccttgatga ttggaatttt tagtatagga attacaaata
     5701 cctatgcttc agaaaattct aatttcaata tcaactccca accaattgaa gaacaagcaa
     5761 ttaattttgc gaaaactgat tttgaacact ccataacttc aattctttta atggaaaata
     5821 acaccgaaac tgatattaac tatactcttg gttcgccatt taaaatgctc gatggagaaa
     5881 atacggcaaa tagttctatt ttctttccag tattttcaga cgctgataat acgattaaat
     5941 atatttatac cgtc
//
LOCUS       DAZKSU010000116         5925 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000116 DAZKSU010000000
VERSION     DAZKSU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.119"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1170)
                     /locus_tag="ACG8Z3_002628"
     CDS             complement(76..1170)
                     /locus_tag="ACG8Z3_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0824881.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="ACG8Z3_002629"
     CDS             1359..2120
                     /locus_tag="ACG8Z3_002629"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0824882.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACG8Z3_002630"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="ACG8Z3_002630"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0824883.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="ACG8Z3_002631"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="ACG8Z3_002631"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0824884.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4812)
                     /locus_tag="ACG8Z3_002632"
     CDS             complement(3760..4812)
                     /locus_tag="ACG8Z3_002632"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0824885.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            4938..5858
                     /locus_tag="ACG8Z3_002633"
     CDS             4938..5858
                     /locus_tag="ACG8Z3_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0824886.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DAZKSU010000117         5727 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000117 DAZKSU010000000
VERSION     DAZKSU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.120"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG8Z3_002634"
     CDS             196..921
                     /locus_tag="ACG8Z3_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824887.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG8Z3_002635"
     CDS             911..1420
                     /locus_tag="ACG8Z3_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824888.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG8Z3_002636"
     CDS             complement(1490..2938)
                     /locus_tag="ACG8Z3_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0824889.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG8Z3_002637"
     CDS             complement(2938..3654)
                     /locus_tag="ACG8Z3_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0824890.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG8Z3_002638"
     CDS             complement(3635..3985)
                     /locus_tag="ACG8Z3_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0824891.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG8Z3_002639"
     CDS             4129..4902
                     /locus_tag="ACG8Z3_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0824892.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8Z3_002640"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8Z3_002640"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DAZKSU010000118         5128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000118 DAZKSU010000000
VERSION     DAZKSU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.122"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..708
                     /locus_tag="ACG8Z3_002641"
     CDS             <1..708
                     /locus_tag="ACG8Z3_002641"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFQ0824893.1"
                     /translation="KKLENNSKEDLFTSKNYVQWMTDVNYIFKTDLKNSIFKDEFEKA
                     YFYKNSRLKNSLYEIISILELIMSKSEGEITSMKEKIFISHSSKDKELAKEFVNFMKT
                     SFEINNRKIFCTSLNGSLGVGDNFITKIKKEIAESAIIIFLITPNYLSSKFCLCELGA
                     AWALDQNIFPILCESASIDDLNATPLLGTQCVKLDDEENMIDLAQMIVSTDLADSQSL
                     LDYKGNLKNFLKLIDAK"
     gene            805..1896
                     /locus_tag="ACG8Z3_002642"
     CDS             805..1896
                     /locus_tag="ACG8Z3_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0824894.1"
                     /translation="MNSDLFKIIEGGIEKDTQKVINYSKKLAEQFYLEGKTSTGKKIE
                     RIIAENNTRVSSLDSLTSRPTDRDTKVDLVDVWIPFETGDTLYFDSIIEKEIKNFTLT
                     YYNRNRLIDHGIESSNNLLLYGPPGTGKSSLAKYIALQLKLPLITVRMDGVISSLLGS
                     TAKNIRKVFDYASKQPCILFLDEFDVIAKIRDDQSEIGELKRVVNSLIQNIDSFSEDS
                     ILIAATNHSHLLDNAVWRRFDKVLFIRIPEYNTRKDIIVEFSNRIENNFCNDSKKLDV
                     LTSLTENFSPSQIKTTIGNSIKKTIVSGDELLKFSEVVYAIFLEINQEDAVNDVDIIK
                     FLHSNNISQREIAEQFSFSLRKVKDCLSN"
     gene            1911..4256
                     /locus_tag="ACG8Z3_002643"
     CDS             1911..4256
                     /locus_tag="ACG8Z3_002643"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFQ0824895.1"
                     /translation="MDSKKSRLISFKQRDQDNLKVGPSPQTQVPTWVLSGADLDRKTK
                     FLIEKLDVIENNFPRFSTQELPDVLSVSFIEDAKAKTHQRKILDLMGSELNGRQIGMS
                     DDNSILLEFLSKDGIAKIKENIDSDQEKYKVSISAIEDFTPYKAKKNLNEKNKNNYLL
                     EFFDYSDKKMNENTFKYVEHLLKKNNIKFEEKIYNEDILLQIDNSSIKDALNLVENDS
                     IPIKSINPIAKTEVSPFRNVLAEAEEESMWVSYDEEKEYPIIGLLDSGVDMNSQTSQW
                     VVKGSGCNIPDEELDTSHGTFIATLLIHGNRYSNYTDCSVDGCRIVDVPIVPRYSIPE
                     NELINNIRDAISNNPEVKIWNLSVSLVEQIDQNSFSTFAIALDEIQDEFKVLICKSAG
                     NDRSFYAGSSAGIISKGAESLRSLTVGSILRNSDNFGFCKKGFPAPYSRIGRGPANII
                     KPEVVHFGGDVFALNAAPTSVEDYQSQGETSYLNAVKRIEAGTSFSTPKIAKSLSEIN
                     NSLIDSDLLLSKTLLIHSSTYFNNPDMKIEDKIKALGFGYPKKSSDILVEDTEYRTTM
                     ILKGELRKSNFIDIMSFPFPKNLLRDGHYYGKIKITLCYDNILDKRMASEYSQSNITL
                     RFGTFEKIEEVRPFANQKVPTNPFKRIGSKNVLLENRYSVNSMRNNTEYASERKLIKG
                     DFKYHSTKQYSIDLSETKPSKLSDLESDKSWFLYMSPEFRNPALDKYSSDQLKMNFCC
                     IITIEDPFKENNIYEGVIKSLEENNFEYNNIEINQDLSINL"
     gene            4784..>5128
                     /locus_tag="ACG8Z3_002644"
     CDS             4784..>5128
                     /locus_tag="ACG8Z3_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFQ0824896.1"
                     /translation="MTGRNPKVFISYSWENKENKEWARELADKLLENGIDAYIDQYDL
                     SLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAELLSGKNERK
                     FIPIIKEGRASEV"
BASE COUNT         1949 a          648 c          880 g         1651 t
ORIGIN      
        1 aaaaaacttg aaaataattc aaaagaagat ctatttacaa gcaaaaatta cgtccaatgg
       61 atgactgacg taaattatat ttttaaaacc gatttaaaga atagtatttt taaagatgaa
      121 ttcgaaaaag cttattttta caaaaatagt agattgaaaa atagtttata tgaaattata
      181 tctattttag aactgattat gagtaagtca gaaggagaaa taactagtat gaaggaaaaa
      241 atttttataa gccatagtag taaagataag gagttagcta aagaatttgt aaatttcatg
      301 aaaacatctt ttgaaattaa taatagaaaa atattttgta cctcattgaa tgggagtcta
      361 ggagtaggag ataattttat cacaaaaata aaaaaagaaa ttgctgaatc agcgattatt
      421 atttttttaa taactcctaa ttatctgagt agtaaatttt gcttatgtga gctaggagct
      481 gcatgggctc tcgatcaaaa tatttttcct attttgtgcg aatcagcaag cattgatgat
      541 ttaaatgcaa cacctttatt aggtacacaa tgtgtaaaat tagatgatga agaaaatatg
      601 atagatttag ctcaaatgat tgtatctact gatttagcgg atagtcaatc tctattagat
      661 tacaaaggta atctgaaaaa ctttttaaaa ctaattgatg caaagtaaag cagtttttta
      721 tggaacgaaa aagcaatata tgggaacaaa tatatgtttt tcgagaacaa attatggtat
      781 attgttcacg aaggggatgt atttatgaat tctgatcttt ttaaaatcat tgaaggcggt
      841 attgaaaaag atactcaaaa ggttataaat tactcaaaga aactagcgga acagttttat
      901 ctagaaggga aaacatctac tggtaagaaa atagagagaa taattgcaga gaacaatacg
      961 cgcgtttcct cgttagattc actaacttct agaccaacag atcgagatac aaaagtagat
     1021 cttgtcgatg tatggattcc ttttgaaact ggcgacacct tgtattttga tagtattata
     1081 gaaaaagaaa taaaaaattt tactttaaca tattacaaca gaaataggct aattgatcat
     1141 ggaattgaaa gttcaaataa tttattgttg tatggaccac caggtactgg taaatcttct
     1201 ttagcgaaat atattgcttt acaattaaag cttccactta ttactgtaag gatggatggg
     1261 gttatttctt cacttttagg aagtacagca aaaaatatca gaaaggtatt tgactatgct
     1321 tctaaacaac cgtgcatttt attcttagat gaatttgatg tcatagctaa aatacgtgat
     1381 gatcaaagtg aaattggcga gcttaaaaga gtggtaaata gcttaattca aaatattgat
     1441 tccttttcag aagatagtat attgattgca gctaccaatc attcgcattt gttagataat
     1501 gcagtttgga gacgttttga taaagtattg tttattagaa ttcctgaata taatacacga
     1561 aaagatataa tagtagagtt ttcaaataga attgaaaaca atttttgcaa cgattcgaaa
     1621 aaattagatg tactaactag tcttacagaa aatttttctc catcacaaat aaaaactaca
     1681 ataggaaatt caataaaaaa aactatagtt agcggagatg aattgttgaa atttagtgaa
     1741 gtagtgtatg caatattttt ggaaattaat caggaagatg ctgtgaatga tgttgatata
     1801 attaaatttt tacattctaa taacatttca caaagagaaa ttgcagaaca gtttagtttt
     1861 tcactgagaa aggtcaaaga ctgtttatca aattgaggac gtgatactat atggattcga
     1921 aaaagtccag attaatcagc tttaaacaaa gagatcaaga taatttgaaa gttggaccat
     1981 ctccgcaaac tcaagttcct acctgggttt tgagtggtgc agatctagat agaaaaacta
     2041 aatttctaat agaaaaatta gacgtgatag aaaataattt ccctcgattt tcgactcaag
     2101 aactacctga cgtactatca gttagcttta tagaagatgc aaaagcaaaa acgcatcaaa
     2161 ggaaaatatt agatctcatg ggctctgaat taaatggtcg tcaaataggt atgtcggatg
     2221 ataacagcat attactagag tttttatcaa aagatggcat agctaaaatc aaagaaaata
     2281 ttgatagtga tcaagaaaaa tataaagttt ctatttctgc tattgaggat tttactcctt
     2341 acaaggcaaa gaaaaatttg aatgaaaaaa acaaaaataa ttatctcttg gaattttttg
     2401 actactcaga taaaaaaatg aatgaaaata cttttaaata tgttgagcat ctgttaaaga
     2461 agaataatat aaaatttgaa gaaaaaattt acaacgaaga cattcttttg caaattgata
     2521 attcttctat aaaagatgcg ttaaaccttg ttgagaatga ttcaattcct ataaaaagta
     2581 taaaccctat cgcaaagacg gaagtgtctc cctttcgaaa cgttttagct gaggcggaag
     2641 aagaaagtat gtgggtatca tatgatgaag aaaaggaata tccgataata ggtcttttag
     2701 attctggtgt tgacatgaat tcccaaacta gccaatgggt tgttaaggga agtggctgta
     2761 atattcctga tgaggagctt gatactagtc atggtacttt tattgccaca ttactaattc
     2821 atggaaatcg atatagtaac tacactgatt gttctgtaga tggctgcaga atagttgatg
     2881 tgccaattgt gccaagatat agtattcctg aaaatgaatt aattaacaat attagagatg
     2941 ctatttctaa taatcctgaa gtaaaaatat ggaatctatc tgttagttta gtagagcaaa
     3001 ttgatcagaa tagtttttct acatttgcaa ttgcattgga tgaaattcaa gatgaattta
     3061 aagtattgat atgtaaatct gctggaaatg ataggtcatt ttacgctgga agctctgcag
     3121 gaataattag caagggtgct gagtcacttc gttcattaac agtaggatca atactgagaa
     3181 atagcgataa ttttggcttt tgcaaaaaag ggtttcctgc tccatattct cgcattggga
     3241 ggggacctgc aaatattata aaacccgagg tagttcattt tggaggagat gtatttgcac
     3301 taaatgctgc tcctacaagt gtagaagatt atcaaagcca aggagaaaca tcatatttaa
     3361 atgccgtaaa aagaattgaa gccggcacga gtttttctac tcctaaaatt gcaaaatctc
     3421 tatctgaaat aaacaatagt ttaattgatt ctgatttact tttatctaaa accttattga
     3481 ttcattcttc aacttatttt aataatccgg atatgaaaat tgaggataaa attaaagcgc
     3541 taggttttgg ttatccaaaa aaatcttcag atattttagt tgaagatact gaatatagaa
     3601 cgacaatgat tttgaagggt gaattaagaa aaagcaattt tatcgatata atgtcttttc
     3661 cttttccaaa aaacttactt agagatggac attattatgg taaaataaaa atcactcttt
     3721 gttatgataa tatattagat aagcgaatgg caagcgaata ttctcagtca aatataacac
     3781 ttaggtttgg aacttttgaa aaaattgaag aagtaagacc ttttgctaat caaaaagttc
     3841 ctactaaccc cttcaaaaga atcggttcta aaaatgttct tttagaaaac agatacagtg
     3901 ttaattctat gagaaacaat actgagtatg cttcggaacg aaaactgata aaaggtgact
     3961 ttaaatacca tagtaccaaa cagtactcaa ttgatttgtc tgaaactaaa ccatcgaagt
     4021 tgagtgattt agaaagtgat aaatcatggt ttctatatat gtcaccagag tttagaaacc
     4081 cggcattgga taagtatagt tctgaccagt taaaaatgaa tttctgctgt ataataacta
     4141 ttgaagatcc atttaaagaa aataatattt atgaaggcgt tataaagtct ttagaagaaa
     4201 ataattttga atataacaat atagaaatta atcaagacct gtcaataaac ctgtaaaaaa
     4261 actagaccta tttttaggtc tagttttttt acaggtttac ataagataca ttatgcgaag
     4321 ttcattattt taaaagctta aataataaac ttttttataa taccaattta tttatgtaaa
     4381 ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc tccaaaacta
     4441 atgactatct attttcaaag aagctagaat gattatctga tcaaatgata atttatattt
     4501 aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg tacacgataa
     4561 agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa aatcactaaa
     4621 ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat tttgatgaat
     4681 tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta ttaccaaata
     4741 gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg gtagaaatcc
     4801 caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg ctagagaact
     4861 agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg atttaagttt
     4921 aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt atgttctaat
     4981 tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg ttgggtatga
     5041 aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat ttatcccaat
     5101 aattaaagaa ggacgtgcaa gcgaagta
//
LOCUS       DAZKSU010000119         4901 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000119 DAZKSU010000000
VERSION     DAZKSU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.124"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8Z3_002645"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8Z3_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0824897.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACG8Z3_002646"
     CDS             complement(2064..3062)
                     /locus_tag="ACG8Z3_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0824898.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACG8Z3_002647"
     CDS             complement(3288..3941)
                     /locus_tag="ACG8Z3_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0824899.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8Z3_002648"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8Z3_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0824900.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1449 a         1021 c          753 g         1678 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaaa c
//
LOCUS       DAZKSU010000120         4875 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000120 DAZKSU010000000
VERSION     DAZKSU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.125"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..142)
                     /locus_tag="ACG8Z3_002649"
     CDS             complement(<1..142)
                     /locus_tag="ACG8Z3_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0824901.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..497)
                     /locus_tag="ACG8Z3_002650"
     CDS             complement(183..497)
                     /locus_tag="ACG8Z3_002650"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0824902.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(548..802)
                     /locus_tag="ACG8Z3_002651"
     CDS             complement(548..802)
                     /locus_tag="ACG8Z3_002651"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0824903.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /gene="prgO"
                     /locus_tag="ACG8Z3_002652"
     CDS             complement(995..1270)
                     /gene="prgO"
                     /locus_tag="ACG8Z3_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0824904.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="ACG8Z3_002653"
     CDS             complement(1242..2195)
                     /locus_tag="ACG8Z3_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0824905.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="ACG8Z3_002654"
     CDS             2807..4300
                     /locus_tag="ACG8Z3_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0824906.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="ACG8Z3_002655"
     CDS             4435..4731
                     /locus_tag="ACG8Z3_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0824907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..>4875)
                     /locus_tag="ACG8Z3_002656"
     CDS             complement(4755..>4875)
                     /locus_tag="ACG8Z3_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0824908.1"
                     /translation="FIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1579 a          815 c          845 g         1636 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaa
//
LOCUS       DAZKSU010000121         4873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000121 DAZKSU010000000
VERSION     DAZKSU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.126"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            500..1660
                     /locus_tag="ACG8Z3_002657"
     CDS             500..1660
                     /locus_tag="ACG8Z3_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0824909.1"
                     /translation="MKITEKNVNPKFETTESNLKKFDLTVTLSNQLVDSIVEMDAMPQ
                     KIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIK
                     IQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLENHFTQYTFDQVV
                     NLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKY
                     KAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQ
                     RDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYP
                     LYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            1695..2462
                     /locus_tag="ACG8Z3_002658"
     CDS             1695..2462
                     /locus_tag="ACG8Z3_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0824910.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            2654..3052
                     /locus_tag="ACG8Z3_002659"
     CDS             2654..3052
                     /locus_tag="ACG8Z3_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0824911.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3359..3739)
                     /locus_tag="ACG8Z3_002660"
     CDS             complement(3359..3739)
                     /locus_tag="ACG8Z3_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824912.1"
                     /translation="MKQTQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKIL
                     FLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIE
                     RNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3761..4111)
                     /locus_tag="ACG8Z3_002661"
     CDS             complement(3761..4111)
                     /locus_tag="ACG8Z3_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824913.1"
                     /translation="MKATHLNYFSRTLINNLLKDTTGISVSTKHFANDLIIFEDNNLR
                     IEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKKAKLLFEFQT
                     DYHNELLAQSEILD"
     gene            complement(4128..4523)
                     /locus_tag="ACG8Z3_002662"
     CDS             complement(4128..4523)
                     /locus_tag="ACG8Z3_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFQ0824914.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(4590..4775)
                     /locus_tag="ACG8Z3_002663"
     CDS             complement(4590..4775)
                     /locus_tag="ACG8Z3_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFQ0824915.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
BASE COUNT         1681 a          719 c          831 g         1642 t
ORIGIN      
        1 tataatatat attttaatct ttaaaacttt agcaacctcc aaaaaggctt tatatcaatc
       61 ttaaatagat tttagggtgg acataaagcg atgcgaacat ggacaaaaag cgatgtgaat
      121 gtggacaaaa agcgatgcaa acgtggacaa aaagcgatgt ggtattccga atatatccaa
      181 aaaaagaaaa agtatgtgaa tttatttcta aaaactagaa aaatatttgt tttttaattt
      241 tggtaatttt tataatttcg tattttattg aaaaggattt ttattttgaa aatgttctaa
      301 aatagcgatt tctgctgtat tttcaatgaa atttgattgg aaaaaataaa atgtgtttta
      361 ctgacattga gttatgttat aaatttacga tacatttctt attgtaacgt atcgaaaaca
      421 ggtctatact tattcaatgg aatcgtacca gttccaagtt tttaagctcg taccaagctt
      481 agaaagaaga ggcaatcgta tgaaaataac agaaaagaat gtaaacccga aatttgaaac
      541 taccgaaagc aatctaaaaa agtttgattt aactgttact ttatcgaatc aacttgtaga
      601 tagcattgtt gaaatggatg ctatgccaca aaaaatttat ctttacgcta taggacatct
      661 tgaccctaaa aatccgcctt ttaataatac cgtcaaagta tctaaaaagg ctttatttgc
      721 gttgatgtct tctagttcat cttcttcaaa acattcaaga ttaaaacaag ttattgaaaa
      781 attgcatcag caatctgtca ttaaaattca aagaactaaa cctaacggta agactgaata
      841 caaagtcatc tcaccgctag aaattactga attcaatgat ttttctgatg cagtatctat
      901 taaatttagt agttcaatta tgcctttatt aacgaactta gaaaatcatt ttacgcaata
      961 tacatttgat caagtagtta acttgacaag caagtatgcg atcagttttt ttcaattatt
     1021 cactaaagat tttaatctgt atgaagcaaa tagagataac ttaacatgga gtacaaaatc
     1081 acttgataga ttaaaaaatc ctgttttctc cattgcagat ttacgaaaga aatttagtat
     1141 tgagaaaaaa tataaagctt ttcgagactt tgaaagacgt gtaattaaag aaccgattgc
     1201 ccaactgaat aaatacactg attttaaaat tgactatgag cttatcaaga agggtcatac
     1261 ttatacaaac ataaagttct tgcttacacg taccatgtca gttccggtac cttataagca
     1321 agaacaaaga gaccctgctt atttaaaagg cgcagaagat aaaacagaga aaatgaatca
     1381 atcgtacttg aaagctatga atagcaaata cactaaatta ctaatcaaaa actttttgat
     1441 cagttctgcc gacttatgcg atcttaaact tatgagccga cttcagaata aagtttatcc
     1501 gctttatgat cgtttatcca aagatagagg atttgaatca gttgtcaatc acctatctta
     1561 cgttgctact cataaggagg attacacaat aaaaaatact gttaaatatc tcgaaaaatc
     1621 aattacgagt tatttgcctt atgtgaacct ttatgattaa caactgaaaa aatgcgtgaa
     1681 ggaaggaaaa acttttgaaa aaagatgaac tgacaaagta caatgaggag ttcttagcaa
     1741 caattgacat gaaaatggat ttaaagtcgt atgaacttta tcaattgctg ttcagttata
     1801 tctcacccac aaagctaaag aaaacaggcg tagtagattt acctgttaaa gcgattatta
     1861 attacatgga tgttgatcaa aatcttgaaa cggaactaaa aaccatagtt aacactttgc
     1921 aaagacaagc agtttatgaa atttcgaatc tagacaaaaa atataagacg tatactactg
     1981 tatctgctat tgatcggatt cattgggatt tagaagagca tagtgtatct ttcagttttt
     2041 cagaagatgt aattccctta attgtctcaa tcaaaaagaa gtatccgttc tttgtttttg
     2101 aagacttagc atcttttcaa aaaaaatata gcatcgtgtt atacaaatat ttggcgatgt
     2161 attataaaaa atatcttatc gccaaaaata aaggagattt tgagaaagct gaaatatata
     2221 agaatcccct gattgaaata gttaaaattc gtgagattac gaaaacagaa aatgagtata
     2281 aaacaatatc gaattttgat agacgtgttt taagggaatc agttgaggaa attaatgaaa
     2341 aatcaatgct gaatattgat tttgctaaag ttagaaaagg cagaaattac aattttgctg
     2401 tccagtttaa tatttctagt gagtttgatg aaaaaactta tagagaaaaa aaattaggtt
     2461 aatgcaaaat atatcttact cgtttgaaga tgcttcagac gtatgctaag atatatagtg
     2521 ccttgggagc aaggtattaa tctcctttag aggttaatta gcttgtgatc gcctatcaat
     2581 ttttttggta ggcgattctt ttttttgaaa caaaaaagca aaaattattt tttaattaga
     2641 gaggtgcaat gcgatgaaat actttttttg tgcagtattc gaatggaaag gaaatgaata
     2701 tgcagttaaa ttcccagatt ttccagactt atatacacat ggtgaaactt tagaagatgc
     2761 ttttgagatg gcgcaagaag tattggaagg atacttggtg cttttagagt tgcaagaaaa
     2821 agaaattcca tcgcctacgc ctgttcaaga gttaaatgca agaatcaagc gatctgagaa
     2881 tgttaattta tttgtcgtaa aagctaatac gaaagttgct aaagctcgaa acaagcaaaa
     2941 ttatgatcga aaatcagtga ctgtggaaca ctatttaaat gttttagcgg ctgaagagaa
     3001 tattaatctg tcgaaagctt tgaatgattt gttgagagaa aagttgattt gatgtctctc
     3061 agatgatcat attaagcaaa aacagcttgt tttaggacgt atggatttaa gaaagacaaa
     3121 ttatcaaaga atatattaaa agctctcaga agccttctga gagcttttaa attgctatgt
     3181 attgtactgc atctgctcaa attgattttc ttttcatgct taccatgtat ctaattttcc
     3241 atcaagtaat ttttagatat gtattgagta ataaatagca aatttgcttg atataagctc
     3301 gaattaggtg ctttgtaatg tgttaggata aaattataca agaaaatgtc aaaagctctc
     3361 agaacgcttc tgagagcttt ttatcggtag gcttgtttaa cgtgatcttg aaggataccc
     3421 ttttgatgtt tctctcgatc cctgcatcgc taattactaa gtgttcttca agtgtgatgt
     3481 aggtatataa attaagcatc aaaatggtgt agataagcaa aaaactccct aaaactaagt
     3541 gttgattata aaaatctgac ttataaaaaa gaactgagaa tgttagtgtt gattcccaaa
     3601 aaagaaacaa aattttgtat ttccaaagat agtggatcaa gcgctgtgct ttacccatct
     3661 caaataattt ataataacct gctaattgat cagcaagcgt atcgttaacg gtttctgttt
     3721 gtgtttgtgt ttgtttcatc tttctcttct cctgtttcaa ttaatctaaa atttcagatt
     3781 gagcaagcaa ctcattgtgg tagtcggttt gaaattcaaa taacaatttt gccttcttaa
     3841 cgaggtttaa agcgttcgtt agtgattcac ttaatgcttc aacatcttta actgattttg
     3901 catgtttcat tttttcagtg tgcgtatagc agaattcagg tattgagtta ggggttgcct
     3961 gttcaataaa catctcaatt cgcaagttat tatcctcaaa aataattaga tcattggcaa
     4021 agtgtttggt actaacgcta atgccagttg tatcttttaa tagattattg attaacgttc
     4081 gagagaaata gtttaagtgt gtagctttca taggttcggt ctccttttta cttttcaagc
     4141 aactcttctt ctagtgcttt ttgaagtagt tgagagaagt ttatgttttt ctccttccct
     4201 aaaagaacta accattccgg aacactaaca ttttttctaa ttgtttttgt atttaagctt
     4261 cttcggaaaa gtttaatgtt tagttggatg attactacca cttggttggc tttcaaagtt
     4321 aattcttcca ctgctgatgg tttaggaaat tctgcatctg gtacactata tagcgctaat
     4381 cctaaagcat ccttagcgtt taaatatgct tcttcaagac tatttccaaa ggtagatatt
     4441 tccggtaagt cagggaaaac gatgttataa gcttctcctt ctttctcgaa gattgccgga
     4501 taaaaaatat tttttgtctt catttcctcg ctccttttct agtgtaagag aggatagtat
     4561 gcctaatacc aaataggaag gcaaggctgt taaagccctg cttgcttcca gatattagct
     4621 tctgttccta gtggtacatc accattgtgg atcggaacaa cgaccgttct tttagtcttg
     4681 gaattataaa attttgcatg actgccagtt tgtccagtct ttttgaagcc gttagatttg
     4741 agaattttta caatttctct tgctgtctta ggcatagcta ccctcccttt acaaaatcta
     4801 ttatacacat tgttgtgtat ggaatcaata gaaaaagagg atgttttaac ccgtggtgct
     4861 agttaatttt ttc
//
LOCUS       DAZKSU010000122         4809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000122 DAZKSU010000000
VERSION     DAZKSU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.127"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ACG8Z3_002664"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="ACG8Z3_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0824916.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            1523..1718
                     /locus_tag="ACG8Z3_002665"
                     /pseudo
     CDS             1523..1718
                     /locus_tag="ACG8Z3_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            complement(3250..3768)
                     /locus_tag="ACG8Z3_002666"
     CDS             complement(3250..3768)
                     /locus_tag="ACG8Z3_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0824917.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4135..4383
                     /locus_tag="ACG8Z3_002667"
     CDS             4135..4383
                     /locus_tag="ACG8Z3_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824918.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1578 a          787 c          732 g         1712 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagggactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tcttttttta attcttcttt atttttatat gttctcatta tattacctcc
     3781 ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg
     3841 ttctacaatg catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa
     3901 atatcccaac gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa
     3961 tatatttctt ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa
     4021 aatcatgtta actactatca attactaata aaaactacca ctttaagcgt taagaactgt
     4081 taagtttagt taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta
     4141 aaactcaaaa tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt
     4201 aaaaaatatt atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa
     4261 cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca
     4321 tattcacgcc tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt
     4381 taggaaattg aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca
     4441 ccagatattc ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac
     4501 cgcgaattta ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt
     4561 aagggatcaa gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa
     4621 acaattatag aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact
     4681 ttcttattct gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta
     4741 cgagtactat tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc
     4801 ccacttagc
//
LOCUS       DAZKSU010000123         4805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000123 DAZKSU010000000
VERSION     DAZKSU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.128"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1200)
                     /gene="nagA"
                     /locus_tag="ACG8Z3_002668"
     CDS             complement(52..1200)
                     /gene="nagA"
                     /locus_tag="ACG8Z3_002668"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0824919.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1276..2112)
                     /locus_tag="ACG8Z3_002669"
     CDS             complement(1276..2112)
                     /locus_tag="ACG8Z3_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824920.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2296..3513)
                     /locus_tag="ACG8Z3_002670"
     CDS             complement(2296..3513)
                     /locus_tag="ACG8Z3_002670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0824921.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3662..3823)
                     /locus_tag="ACG8Z3_002671"
     CDS             complement(3662..3823)
                     /locus_tag="ACG8Z3_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824922.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3896..3979
                     /locus_tag="ACG8Z3_002672"
     tRNA            3896..3979
                     /locus_tag="ACG8Z3_002672"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3930..3932,aa:Leu,seq:gag)
     gene            complement(4219..>4805)
                     /locus_tag="ACG8Z3_002673"
     CDS             complement(4219..>4805)
                     /locus_tag="ACG8Z3_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824923.1"
                     /translation="LDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFG
                     NTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIV
                     GIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAV
                     KELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1459 a         1001 c          775 g         1570 t
ORIGIN      
        1 accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg
       61 gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat
      121 aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat
      181 tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac
      241 aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag
      301 acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg
      361 catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc
      421 cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa
      481 gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg
      541 cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc
      601 ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt
      661 gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc
      721 tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt
      781 ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt
      841 agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc
      901 tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc
      961 agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg
     1021 aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac
     1081 ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata
     1141 gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat
     1201 tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag
     1261 aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc
     1321 atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa
     1381 taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac
     1441 ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa
     1501 aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct
     1561 tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat
     1621 acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta
     1681 gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc
     1741 gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga
     1801 aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact
     1861 atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac
     1921 atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag
     1981 ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat
     2041 tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat
     2101 taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac
     2161 tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt
     2221 aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa
     2281 atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc
     2341 tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt
     2401 ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac
     2461 gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa
     2521 agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata
     2581 ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc
     2641 gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga
     2701 caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat
     2761 ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac
     2821 gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg
     2881 atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc
     2941 ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat
     3001 agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc
     3061 ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca
     3121 caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat
     3181 acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt
     3241 cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt
     3301 ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt
     3361 tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc
     3421 ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc
     3481 ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa
     3541 gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa
     3601 attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa
     3661 cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa
     3721 tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta
     3781 agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca
     3841 tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga
     3901 tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt
     3961 tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg
     4021 tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta
     4081 caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa
     4141 cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag
     4201 cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag
     4261 cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc
     4321 tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat
     4381 tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat
     4441 catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac
     4501 aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa
     4561 aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat
     4621 cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata
     4681 aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca
     4741 tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc
     4801 taaaa
//
LOCUS       DAZKSU010000124         4780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000124 DAZKSU010000000
VERSION     DAZKSU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.129"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1257)
                     /locus_tag="ACG8Z3_002674"
     CDS             complement(<1..1257)
                     /locus_tag="ACG8Z3_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0824924.1"
                     /translation="MTINEVIYSPKHPLFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLGNLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPLYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERYPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACSGLAEIFEA"
     gene            complement(1308..2129)
                     /locus_tag="ACG8Z3_002675"
     CDS             complement(1308..2129)
                     /locus_tag="ACG8Z3_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0824925.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLGCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(2126..2935)
                     /locus_tag="ACG8Z3_002676"
     CDS             complement(2126..2935)
                     /locus_tag="ACG8Z3_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0824926.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFVMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(2956..3450)
                     /locus_tag="ACG8Z3_002677"
     CDS             complement(2956..3450)
                     /locus_tag="ACG8Z3_002677"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0824927.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(3462..3962)
                     /locus_tag="ACG8Z3_002678"
     CDS             complement(3462..3962)
                     /locus_tag="ACG8Z3_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0824928.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(4026..>4780)
                     /locus_tag="ACG8Z3_002679"
     CDS             complement(4026..>4780)
                     /locus_tag="ACG8Z3_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0824929.1"
                     /translation="FKQIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKD
                     VFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYD
                     ILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETV
                     ISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSR
                     SIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         1481 a         1087 c          782 g         1430 t
ORIGIN      
        1 ggcttcaaaa atttctgcaa gtccactaca ggccttccaa agacaaagat tgctgccggt
       61 atgatacttt ccaagtgcaa aggcgtctga taaccaaaca gatggaaaaa gcattggttc
      121 ccctgaccga cgttgagcta atatgtcctc aataattctt gaagcattca gtagaacctg
      181 cggataccgc tcaaagaatt ttgtatcccc agtatgttca aaataaagac tagccagtaa
      241 cgcactggat aacgaattac tcagagaatg attgatacct cctgtaaatt ttgaaccagg
      301 aaacttaacg ccatattgat caaaccatag aaccgttttt tgacataact ccggttcaaa
      361 catggtgaat ggcaatgatg cgtaatacat atccttattc caaattcgat tagtagcagg
      421 atagctgccc caattgctgc cgacgacctg tccctgtcga ttcataccga aactagaaaa
      481 gctttgataa agtgcacgac gatagagatg gacaactcta tcgtttggca ttgacagctg
      541 cccaaatagc tgagcaaaat agttcaatgt ttccttcagc catttttcga tatcagattc
      601 ctgaaaagtc attacttctt caaaagcatt tgggtccaca aaagcaatgg cgaagcttgc
      661 cacttcctgt tgtgcgatcg tgtccttatt tcctttttga gaaatcaaca cattttgctg
      721 atccgaatac ttctcttggt acaaaggcac atctttcact gaaacttgaa ggctttcgtg
      781 tgtttcattc acaatgtaaa cctgttgaac aagcatactt aaacgcttat tagccagtat
      841 tggagcaaag gatactacaa tgaccttaaa cgtcgaataa tgatgaatat atctaggcaa
      901 acagtcttga atcacatcga cttcaatacg atgatccgat tctgacaaat ggtagacaat
      961 cccgtttact tcaaccttta aggataacga attggtttga gaaagctgtt tattgaccca
     1021 aacacctggt ttttcctcag ttagtaaatt ttctcgataa aaggtcaacg tttgaatcgt
     1081 cccgcaaaca tcaaggtttc ctaatgtctt ttgattactt acatcgaagg tcaatccctt
     1141 tacatcagga atattccgat agtagctgta ttcgtttgaa gtagtcacat ttccgtcgtt
     1201 taactcagca ttaaataatg ggtgtttggg tgaataaatt acttcattaa tagtcatctt
     1261 ttccctcctt gctaaaaaaa ctgcagccta gtagctgcag cttcctacta tgcgattcca
     1321 agtaatgaca ataaaattcc caagacgacc attccaaaaa taattttgat gacgccgact
     1381 tttttatcta gtaatttgaa acagcccaat gttaccaata acggtacaat tcctttaaaa
     1441 atactatcga ggatttcttg gacattcatt agttcttcgc ccttcattga aaagctcaaa
     1501 acggtattga atttgaccat actgatcgtc attgcgccta ccatgatcag acccaagata
     1561 ctagcagctt ttgtcagaat ttcaattaac ccgttcgcat acagcttttc aatatattta
     1621 gagcctaatg aatagcctaa aacgccgcca tagtatcgca tcaaataatt aggtatattg
     1681 aacataacta aaaagataat tggtgccaaa atatttccgg cattggctaa gttgatcgct
     1741 agtcccgcgg taacgacacg ccaaactccc cagaacaatg aatcgccaat tccagccaat
     1801 ggtcccatca aactcgtttt gatcgcgttg atactttcca cgtcaaaacc agctttttca
     1861 gaattttcct tctccatcga tgccgcaatc cccatgacaa aggacgcgca aggaatggtc
     1921 gtcgaaaaat actgtgtatg acgttccagc gccgcagctc gatcttcttc acttttgtag
     1981 aaatgattaa tgaagggcat catagaatag gcgaaaccac cgccgcccat cgtcaccgga
     2041 ttcaccgaag catacatagt catcgagcgc caaaacatct tccgaaccaa ttttttctca
     2101 tctttagaaa ttccatcagc taatttcatt gaaagaaatc ctcctcttct tggtcaagcg
     2161 ttgcatttac agcagcatcg ctttgaactg catgaacaga tatatttttt agttgtagat
     2221 ctctttgggc tataacaatt gcaataatcg ttccaactgc ggcaattgca atcaatggta
     2281 actcgagata cgccataaca acggccccaa agaaaaacca cacagcaata tttttactcc
     2341 aaagcatccg taacagcata gccatcccaa ctgccggcag cagatttcca gcgactgtta
     2401 atccgttcat gatgacctct ggaatattat ccaagacgct tttgacaaca tctgctccaa
     2461 ataaaacggc aaagaatgga ataagagcat aaagcgcata gtggacaaac cacataacga
     2521 agtgcagatt gatgattcct ttttgatcac cagattttgc aaatttgaaa aatttagcag
     2581 ccatcggtcc acatacaaaa gaaaaaatca tattcattaa ctgtaatcct aatactgcta
     2641 gtggaaagac aatgggaata atcgcatcca cgtttcctcc cattttgatt gcgaacgctg
     2701 tagccagccc agcagccaaa gaaggctccg ccgtactaga gattccgata ttgacgacac
     2761 ccataaagat ggcttctagt gacgccccga caatcaaccc cgtctgcaaa tcccccatca
     2821 acgctcctgc taacggagca acaacaatcg gacgactcag catggacatc ccgaacattt
     2881 catgtccagc aactccaata aaaacggcca aagcgactac aaatgcatca tacataattt
     2941 ttctccttcc tttttctaaa aatcactcaa aatagagata gctgaacgtt tttccatcgc
     3001 cggtgtaggc tgaaaaacgg tatctggata aagttcaact aattctttta agtcttgaat
     3061 ttctgacgtc gttaacatca cttcctttga taaggttttc ttatcttccg atggcggtgt
     3121 cattttccca gcattcccca cgttcattaa ttcgatttcc gttgggaaag ctttcgcaac
     3181 ttttaccgca tctttgacat tagggactaa aaccatcaag cgcatgtcct tcgcccgcgg
     3241 atcttttaac aaatgaatcg cgtcatcaac tgttttaata atgactttga cattcgatgg
     3301 tgcagccatc ttcaatgtca tcttttgaat atcgttggta gccgcctcat cgcttgctac
     3361 tactaacccg gttaaattta gactctttgt ccaagtcatc gctacttgtc catgaattaa
     3421 tcgttcatca attcgcactg ctttaatcat ttttcttcct cctaaaaaaa atcttcctct
     3481 tcatttacta catgtacctc atcaagaatg gtcatcttta attcctgttg gcatttattt
     3541 accagttcct gaatatcctc ctttgttaat tccttggttg ccaatactat ttcgagcaga
     3601 attggcagat taaagccaga caaaatagtt acagcaatct ctttctccaa aaaggttttg
     3661 gtaatcttct gattgacact tccgccaaat aaatcggtaa aaacaaaaat ctgatcctgc
     3721 tcgttcacct ggtttacaaa tacattcaac tcctgttcaa aatcacactc atccacataa
     3781 gcattaatca ccgtaatctc ttgaaccttc cccgtcagta attgaatact gctaagaaag
     3841 cctgaagcta agtgaccgtg agtcgctatt agaattttct tcatatggtt aattcctttc
     3901 tttctgttca cttttatata agcaattcct gtgccaactt ttaaaaacat tggtttaatc
     3961 acgttaattc acattaaaaa aagtgtctaa cctaaaaaaa cagattagac acttttttag
     4021 acactttatt tttgaatata cggtcctaat aattcataga gcagcgaaag ctcataagca
     4081 ttgactttaa aattatattt catctccatt gattgaaaaa tacttctaga aacataataa
     4141 aattcatctt catctggttt caaatcataa ttcggttctt cagatttttc tgtccgagcc
     4201 aagaataaac gttccatcat caatgccgta tgcatataga gatttagttt tacacggcca
     4261 tttaaagtga aatgatagta attttcatat ttagaaatga ctgtttccac ttcactcatc
     4321 acgatcgttg ggttcagaaa gttcaaacgg tccgcaattc cttccaacga aaagaatttt
     4381 actagctcct gaatcaagcg gttaaattct gccggcttca tttcaggact caaaatatgc
     4441 tccaaattgg ctgctccatc accgtctaag atgtcataaa cattaatatt aggaattgaa
     4501 aaggaacgtg gaagatccga agtcgtgatg accaattttg tcttgtcaaa ataagtttca
     4561 tcattttggt cgattaattc acgtagttct ctgtagtcct tcgtgaaaac ttcgatggat
     4621 tcatccgata aatgatgacg aaaaacatct ttcaatttgt ccgaaatccc caaacccgac
     4681 atacaggaaa tgataatgtt gctactttga gaaaagcctt caaagtattg aacattgatt
     4741 gaatacttgt ctgctgcatc ctccgcaatc tgtttaaatg
//
LOCUS       DAZKSU010000125         4612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000125 DAZKSU010000000
VERSION     DAZKSU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.130"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..373
                     /locus_tag="ACG8Z3_002680"
     CDS             143..373
                     /locus_tag="ACG8Z3_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0824930.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            470..1771
                     /gene="murA"
                     /locus_tag="ACG8Z3_002681"
     CDS             470..1771
                     /gene="murA"
                     /locus_tag="ACG8Z3_002681"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0824931.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1784..1960
                     /locus_tag="ACG8Z3_002682"
     CDS             1784..1960
                     /locus_tag="ACG8Z3_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0824932.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2095..2391
                     /gene="yidD"
                     /locus_tag="ACG8Z3_002683"
     CDS             2095..2391
                     /gene="yidD"
                     /locus_tag="ACG8Z3_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0824933.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2576..3043
                     /locus_tag="ACG8Z3_002684"
     CDS             2576..3043
                     /locus_tag="ACG8Z3_002684"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0824934.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3128..4012
                     /locus_tag="ACG8Z3_002685"
     CDS             3128..4012
                     /locus_tag="ACG8Z3_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0824935.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4126..4362
                     /locus_tag="ACG8Z3_002686"
     CDS             4126..4362
                     /locus_tag="ACG8Z3_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0824936.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4450..4530
                     /locus_tag="ACG8Z3_002687"
     tRNA            4450..4530
                     /locus_tag="ACG8Z3_002687"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4484..4486,aa:Tyr,seq:gta)
     gene            4537..4608
                     /locus_tag="ACG8Z3_002688"
     tRNA            4537..4608
                     /locus_tag="ACG8Z3_002688"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4569..4571,aa:Gln,seq:ttg)
BASE COUNT         1563 a          715 c         1012 g         1322 t
ORIGIN      
        1 tttgcatatt tttagcattt atttcctaaa aaatgtaagg ggattcctat atttttaagt
       61 atacttgttt ttccttttat acattttatg gtattatcat tttattagtt attataaaac
      121 aatcgttaga aaggacgtca ttatgcaagt atacggaatt gatgcggtgc ttcgaatcgt
      181 gagtcatttg gcgtttatct acttagcctt ctggtcttta cgttcgttac gcattgaaac
      241 ttttttcaga ccaatgcata atacgcaaat tagaatggca atcgtacttt tttcaatttt
      301 tttagggtac actgcaagta attttttctt agaagttatc gctttatgcc gaaatctttt
      361 cgttagcctg taaattttaa aaaaaggtac tattttaaaa gcaaggatgt tataatagaa
      421 aagattttga gtaagtgtat gctcattgga attttttgga gggaacaaca tggaagagat
      481 catcgtaaga ggtggcaatc aattaaatgg aacagtaaga attgaaggag ctaaaaatgc
      541 tgttttgcct attttagctg ctagtttgtt agcagaagaa ggtattacga cattagacaa
      601 cgttccgatt ctttctgatg tcttcactat gaatcaagtg attcgtcact taaacgtaga
      661 tgtagatttt gatgaacaaa agaatcaagt aacaatcgac gcttcaagac aattagaaat
      721 cgaagcgcct tatgagtatg ttagtcaaat gcgtgcttca atcgttgtga tgggtccgct
      781 acttgccaga aatggacatg caaaagtagc tatgcctggt ggatgtgcga ttggtaaacg
      841 accaatcgat ttgcatttaa aaggatttca agctttagga gcaaaaatta ttcaaaaaaa
      901 cggctatatc gaagcaattg cggatgaatt gatcggtaac acgatttatt tagatttccc
      961 tagtgtaggt gctactcaaa atatcatgat ggctgccgta aaagcaaaag gaacaaccat
     1021 cattgaaaac gtagctcgcg aaccagaaat cgttgattta gcaaacattt tgaataaaat
     1081 gggcgctcaa gtctatggag ctggtactga aacaatgcgt attgaaggcg tggatcattt
     1141 acatgcagtc aaccactcaa tcgtacaaga tcgtatcgaa gcaggtacct ttatggttgc
     1201 tgctgcaatg acacaaggca atgtgctgat tgcagatgct atctctgaac ataaccgccc
     1261 attgatttct aaattgatcg aaatgggtgc agaaatcatt gaagaagaag gcggagtccg
     1321 tgtcatcgga ccaaaacata tcttacctac agatgtaaaa acgatgcctc atcctggttt
     1381 ccctacagat atgcaagctc aaatgacagc tatccaatta gttgcagaag gtacaagtgt
     1441 agtgactgaa acagtgtttg aaaatcgttt ccaacattta gaagagatgc gccgtatgaa
     1501 cgctcatgtc aaaatcgacg gaaatgtagc aatcatggat ggaaaccatg aattgcaagg
     1561 tgcagaagta tatgcaacag acttgcgggc agcagcagca ttagttttag ctggcttgaa
     1621 agcaaatgga atcacacgtg tgcgtaactt gaattatcta gaccgtggat attataattt
     1681 ccatatcaaa ctacagcaat taggtgcaga cgttgaacgt gtagacatgg atcaaacttc
     1741 tgctgaaaaa acagcacaaa cgattgctta aggaagtggc tgaatgagta accaacgata
     1801 tatcttatta actttgataa agattttaat ggtgatctta ttgcttatcc ttctttttgt
     1861 ggccggaacg atgatcggat atggtgtcat cggtggcggc aatccattta aagtatttca
     1921 gccaagtttg tggattcata ttcgagactt ttttcattga taaataagaa tcacagtggt
     1981 tctagtcgat atgaagtgag ccatgaagca gaacatgata aaagaaaaca gaagggaacg
     2041 ttgacgttag tcgcgatccc tttttttagt aaaagaaaag aaggaatcta attgatgaag
     2101 aagatattga tttatttagt tagaggatat cagcgcttta tttcgcctgc atttccacct
     2161 agctgccgtt attacccaac ctgttccaat tatatgataa aagcaattca aacccatggc
     2221 gcgctaaaag ggacggtaat ggggactgca cggatcttac gctgtcatcc ttttgtcaaa
     2281 ggcggtatcg attatgtacc attgacattc cgattaacgc gaaataagaa agataaaaaa
     2341 gaaccagatt ataaaaaggt tgtgaaaaat acgggtagca ctgagcgata aagaaacaat
     2401 ttggaaaata gcttcttata ttttgcaaaa ttgtgagtta ttgccgaagt gttggatttt
     2461 gaacaccgtc aaaaaaaagg ttgtgaaaaa tacggctagc actgagcgat aaagaaaaag
     2521 aggaaattta ttgtcaaagt aacaactccg caactactta tatgggggac aaaaaatgat
     2581 cgaagaacta aacaagaaag atttaccatg gtccttgtta ttagatgcag atcctgataa
     2641 agaaaaagtt caagtatatg tttccagagg gagtggctgg atatggaaag aaaaagaaaa
     2701 aacaataggt gtactgatct atgtggcaag ggaaaaagaa tacgaaatcg tcaatgtagc
     2761 agtaacccca acgtatcaag gaaaggggat tggcggaaaa ttacttgaga cagcttttca
     2821 gaagctttca caactcgctt ccagtcaaac tcagatcatc atacgaaccg gcagtactag
     2881 cagtgcggcg ttacatctct atcaaaaaat gggctatgtg gagattggtc gagtcaaaga
     2941 ctattttatc catcattact ctgagcccat ttttgaaaat ggagatcaat taagggatca
     3001 agtgatttta gcaattcact tacctaaaaa tagctttaac taataaaatt tcaattcaag
     3061 aaaatgcttt caaatgctaa atagatacat tacaatagct ttagcaaaca aaaaggagaa
     3121 tatttttatg ggaatattag tttttgattt tggtggatcg gctgtcaaat atggttgttg
     3181 ggatggcaaa gagattaaag gtaagggaca atttgcaacg cctgaatctt gggaagaaat
     3241 gaaagcgcaa ttatttcaag tttataaaaa aatactcgtt tcttttgaag tcgaaggaat
     3301 tggaatcagc tctcctggag tagtgaataa tgagaaacag gtgattgagg gaatcagtgc
     3361 gattccttat attcatggat ttaatatttt ccgagatttg gaaactctgt ttcaacttcc
     3421 agtaacgatt gaaaatgacg ccaactgtgc cggaatggct gaattttatg aaggtgctgc
     3481 ccgcgattat caagatgttg cttttgttgt ggcaggaact gggattggcg gggcgttgtt
     3541 cacgaaagga caaatcaata aaggggcaca tctttacggt ggagaatttg ggttgatgtt
     3601 cttaaatggc gataagacat tcagtaaact tggtacagcg gtacagatgg cttggcgtta
     3661 ttgtgaacgc aaaggtctcg ataaaaatgc ttataccgga aaagatgtct ttgaattagc
     3721 agaaactgga gatgcaatag caaaagaaga agttgagagt ttttatacgt atcttacaaa
     3781 gggattattc agtattcaat tttcgtttga tccagaagtg atcgtattag gcggcggggt
     3841 ttctgcaaaa gaagggctca ttgatgagat caaaagccga atgaagaagc tgacagaaca
     3901 attcgatctg catgatttcg aaccgcagat tttattatgt gaatacagaa acgatgctaa
     3961 tcttgtaggt gcagcagcaa actatctcgc tgtacgtcaa ggggagaatt aagagcttta
     4021 gagaaggtgc agcttgcata aaagaagtga atgaaatgaa taggagattt ttacctaagg
     4081 aatgttttat ttgctgcagt atgataaaat atctgagagg tgaaaatgat gtcaaaaaaa
     4141 aataaagata ttgaagtgcg tatagaagaa gtgaaaaaag atatcaaagg taagaattat
     4201 gaagtcactc agttatttat agggaaaaaa atgattggtg agatcttagc ctatggacca
     4261 aaagaatatg aaattttctt tggtgaagaa gattttggaa aagaaaaaag tcttgaaaat
     4321 gcgatagaaa cagtgattcg acactggaat ctgcacgaat aaaaaaatat caaaaaaact
     4381 ttcaataaag ggttgcaaaa atctggaaaa ttaggtatta taactaagtc aggttgatac
     4441 aaacctttcg gaggagtagc gaagtggcta aacgcgacgg actgtaaatc cgttccttag
     4501 ggttcagtgg ttcgaatcca ctctcctcca tttcattggg gtatagccaa gcggtaaggc
     4561 aagggacttt gactccctca tgcgttggtt cgaatccagc taccccagta ta
//
LOCUS       DAZKSU010000126         4603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000126 DAZKSU010000000
VERSION     DAZKSU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.131"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..113
                     /locus_tag="ACG8Z3_002689"
     tRNA            43..113
                     /locus_tag="ACG8Z3_002689"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75..77,aa:Cys,seq:gca)
     gene            134..218
                     /locus_tag="ACG8Z3_002690"
     tRNA            134..218
                     /locus_tag="ACG8Z3_002690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:168..170,aa:Leu,seq:caa)
     gene            280..352
                     /locus_tag="ACG8Z3_002691"
     tRNA            280..352
                     /locus_tag="ACG8Z3_002691"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:313..315,aa:His,seq:gtg)
     gene            362..433
                     /locus_tag="ACG8Z3_002692"
     tRNA            362..433
                     /locus_tag="ACG8Z3_002692"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Gln,seq:ttg)
     gene            451..535
                     /locus_tag="ACG8Z3_002693"
     tRNA            451..535
                     /locus_tag="ACG8Z3_002693"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:caa)
     gene            790..1665
                     /locus_tag="ACG8Z3_002694"
     CDS             790..1665
                     /locus_tag="ACG8Z3_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0824937.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1769..2533
                     /locus_tag="ACG8Z3_002695"
     CDS             1769..2533
                     /locus_tag="ACG8Z3_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0824938.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            2520..3962
                     /locus_tag="ACG8Z3_002696"
     CDS             2520..3962
                     /locus_tag="ACG8Z3_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0824939.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3973..>4603
                     /locus_tag="ACG8Z3_002697"
     CDS             3973..>4603
                     /locus_tag="ACG8Z3_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0824940.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIY"
BASE COUNT         1462 a          717 c          994 g         1430 t
ORIGIN      
        1 ctataattta ttctttattt tatagattga taatagtata atggcggtat agccaagtgg
       61 taaggcagag gtctgcaaaa ccttcatcac cggttcaaat ccggttaccg ccttagtact
      121 aataaatcaa cttgccggcg tggcggaatt ggcagacgcg ctggactcaa aatccagtgc
      181 ccgttgaggg cgtgccggtt cgaccccggc cgccggtatt gactaagatg tcttcatctt
      241 agttttttat tgagaaatta aataacatct tttatgatgg cgaatgtggc gaagtggtta
      301 acgcaccgga ttgtggctcc ggcattcgtg ggttcgattc ccatcattcg ccctttattc
      361 ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt tggttcgaat
      421 ccagctaccc cagttattat ttttaaattt gccggcgtgg cggaattggc agacgcgctg
      481 gactcaaaat ccagtgtccg ttgaggacgt gccggttcga ccccggccgc cggtataatt
      541 atcaatatag caactttctt atagagagtt gctatatttt ttttgttttt atgtaattac
      601 ctccaaatca attcgataca agtattatca taaaacgagt aaaaaagata ttgataacgc
      661 ttttattatg tgttaaacta tttttgtaaa ttatgtaggg tgctactatt caattaggca
      721 cgacctaaga aagggttctt tcttaggtcg ttttttgtct ttaaaaattg tttggagaca
      781 aaaatatgta tgaaggagtc tgcaagttgt tttgttttat catatagagg aggtgaatta
      841 acggtgtatg tgatgaaaaa gctgaatcat aatgctgtat tgatcgatga cgatggaata
      901 gaaaaaattg cgatggggaa agggattggc ttttctgcta aagtgagtaa gccatttgat
      961 gttgaggatg ctgacaaatt atttgtatta gattctaaag aaaagatcaa acgattcagt
     1021 gagatagtct cgcagattcc aatcgaattt atggattttg cggaagaagt gattgaactg
     1081 ataacagcta ggatctcctc tccattagat tcaaatatct atattacatt gacagatcat
     1141 atttattttg ctattcaacg aaaaagagaa gatagagatg tcacagcaat tatgttgcca
     1201 gaaatgagat tcctttatcc aaatgaatat gatatagcag tagaagtggt cgagcgaatc
     1261 aatcatcgat atcaaaccga gttagggtca aatgaagtag gattcatcac tatgcatatc
     1321 attaatgctg agctaggaga gcctaatagt ttaaagagct taaaaatagt agaaatgacg
     1381 accacgattt tgtcccatct agaaaaatcc tattcaaacg tttatgataa agaatcactg
     1441 acttatcatc gcttaatggt tcatatcaaa ttcctggtta aacgcttgat ttatcaggaa
     1501 gaaacgccag tagaaaatat tggttttttt actgaaacat ttaaacatag caaagcgtat
     1561 tgtacagcac taaagattaa agatatcatc gaacagcaat ttcaacttgc cattcaagaa
     1621 aatgaaatca tctatttggc aattcatcta gcaagaatct attaaaataa gggcgtaact
     1681 atctataggc gcaaccaaat gacacatagt aaatggacta tgattatttg gttgcgccta
     1741 ttttagtaga aaaaagaagg agaatgaaat gggaaaagtg atttttaatt cggatgattt
     1801 tggctatagc catggtgtga attatgggat tatggatgct tatcagcgag gaattttgac
     1861 atcaactaca ttgatggcaa atatgccggg tttcgagcat gctgtgaaac taagaaagga
     1921 aatgcctagg ctaggtgtag gagtccattt aacattgacg tgtggcaaac cactcctcaa
     1981 aactgtcgat actcttatcg aaagcggaga gttcagatcc ttatcttatt atcagcatcc
     2041 atttaacatt gacaaagatc aactgtatca ggaatggaat gctcagattc aaaaaattta
     2101 tcatgcgggg atcataccaa ctcatttaga cagtcatcat catactcata ctttggatga
     2161 aaatcaggag gtagttgtta cattagcaaa aaaatatgat ttgccggttc ggagcaactt
     2221 tgaacgaaaa gatgaagttc gtcatgtcga ttattttgag ccttgtttcg atgtcgtagg
     2281 agaaactgat gaaaaaaaac gacaaatcaa tacgagtgtg gaagaatatt tggcaagttt
     2341 actcatgata ttgagagaag ataaaacgat cgaagtcatg tgtcacgcag cgtatttgga
     2401 tcaggaattg ttaaatggtt caagttttgt gtatcctcgt acccaccaag tcgaattttt
     2461 aattcattca gcatttgctc aaaaagtgaa aaatgatcct ttgattgagt tagtaacata
     2521 tgaggatatt taatagagga ggaataaaaa tgaatgttaa atcaaatgtg cagaagcttg
     2581 gaagagcgat gctacttcca gttgcagcga tgccattagc gggattgatc atgcgtttat
     2641 cagctgacga tatgctgaac atcccagtaa ttggtgcagc tggaaatgcc gtttttggaa
     2701 acttggatat cttatttgct ataggagttg caatcggttt tgtcaaaaat aaggacaaag
     2761 gaatacctgc acttactggt tttttagcga ttgctacctt gaaaaaaggc ttagaaatta
     2821 tggacccaga agtgaatatg gggatttttg gtggaattat ttccggttta gtagcggcat
     2881 ggacgtataa tcgctttaaa gaacaaaaat taccagtagt tttttcttat tttgcgggcg
     2941 aaaaattccc attgactatg gtgatgattc tacaaacaat cgcagctgtt tttttgggct
     3001 ttatctggcc gctcatccaa attgggattg ataattttgc aaaactgctc gttgatatgg
     3061 gagcattagg tgtggggata tttatgtttt tgaatcgttt gctgatcccc tttggactgc
     3121 accatgtgtt gaatacgtat atttattacg atttaggaag ctatacatct cctagcaagg
     3181 aagttttcag aggagagatg acacgcttta tcaacggaga tcctcaggct ggactgtttt
     3241 tatcaggttt ttttgtagta atgatgtttg gtataccagc tatatgtcta gctatctata
     3301 aagcagctta taaagaaaat aaagagatgg tcaaaggaat catgggaagc ggtgcaggaa
     3361 cttcatttat tgcaaatatc actgagcctg ttgaatttag tttcatgttt ctttctccta
     3421 tgttgtacgt gatccatgca ctttttgccg gtttagcagg gattgtctgt tacttattca
     3481 atatacgaat tggttttaca ttcggagcat gtattgttga ttatctcatc aattttcgaa
     3541 tcgctaccaa tgctattttg attcttccaa tcggaatttt cttttttgcc ttgtattacg
     3601 ttacttttta ttatttgatc aataagagaa acattcagac attaggacgt gaagctaagg
     3661 cagagtttgg caatgaagta accttagaag aaacagagtt agggttagca agtaaaaatt
     3721 atgaatatat ggcaacaaaa atgctacaag cgtttggagg aaaagccaat atcctggatg
     3781 tctatagttg taatacacgc ctacgggtag aggtagtgga tcccaccatg gtcgaggaac
     3841 agcgaatcaa gcaattaggg ataagcggga tcattaaacc aactgaaaaa aattatcaaa
     3901 ttatcatcgg cttagaagtg acttatgtta tggcagaatt caataaatta ttagaagaat
     3961 agaaaggaaa agatgatgat gaaaaaacag attatcacta tagctggagg cgggagtacg
     4021 tatacaccag gaatcgttca agccattttg aacaatgaaa aacggttgcc attatctgaa
     4081 atacgtttat atgatattga tgaagaaaga aatatggata tgtacttgat tgttaaattt
     4141 atgctgaaaa gaaaaggttt ttcaaatatt cgcattcgtg caacggatga tccaaagctt
     4201 gcttttacag gatgcgattt tgttttctca caaattcgtg ttggaggatt ggagatgcga
     4261 gaaaaggacg aaaagattcc acttaaacat ggattagtag gacaagaaac gtgtggactc
     4321 ggaggatttg cttatgggat gcgctcaatc aaaggtttat tggaaattgt cgatcatata
     4381 caagactatg cccccaaagc atggattttg aattatacaa atccagagtc gatcgtttct
     4441 gaagctgttc gtcgtaaata tccgaatgta aaaatgatta atgcctgtga tatgactatt
     4501 tcaattgaag aaacgattgc agtgaattac ggttatgatc gtaagaattg gatcgttacg
     4561 tattatggtt taaaccactt tggctggtat acatcgattt atg
//
LOCUS       DAZKSU010000127         4348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000127 DAZKSU010000000
VERSION     DAZKSU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.132"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..93
                     /locus_tag="ACG8Z3_002698"
     CDS             <1..93
                     /locus_tag="ACG8Z3_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFQ0824941.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="ACG8Z3_002699"
     CDS             complement(151..1371)
                     /locus_tag="ACG8Z3_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0824942.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="ACG8Z3_002700"
     CDS             complement(1391..2002)
                     /locus_tag="ACG8Z3_002700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0824943.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="ACG8Z3_002701"
     CDS             complement(2045..2977)
                     /locus_tag="ACG8Z3_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0824944.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="ACG8Z3_002702"
     CDS             complement(3398..4258)
                     /locus_tag="ACG8Z3_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0824945.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1241 a          874 c          721 g         1512 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa agctttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgaga attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaattgg ttaagata
//
LOCUS       DAZKSU010000128         4305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000128 DAZKSU010000000
VERSION     DAZKSU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.135"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1137
                     /locus_tag="ACG8Z3_002703"
     CDS             148..1137
                     /locus_tag="ACG8Z3_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0824946.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="ACG8Z3_002704"
     CDS             1134..1553
                     /locus_tag="ACG8Z3_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824947.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="ACG8Z3_002705"
     CDS             1562..1870
                     /locus_tag="ACG8Z3_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824948.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="ACG8Z3_002706"
     CDS             1874..2236
                     /locus_tag="ACG8Z3_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824949.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="ACG8Z3_002707"
     CDS             2458..3864
                     /locus_tag="ACG8Z3_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0824950.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DAZKSU010000129         4233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000129 DAZKSU010000000
VERSION     DAZKSU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.136"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..360
                     /locus_tag="ACG8Z3_002708"
     CDS             <1..360
                     /locus_tag="ACG8Z3_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0824951.1"
                     /translation="FFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACN
                     NAYCINEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVS
                     EINNFLNEESSDERKQI"
     gene            341..1075
                     /locus_tag="ACG8Z3_002709"
     CDS             341..1075
                     /locus_tag="ACG8Z3_002709"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0824952.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1108..2016
                     /locus_tag="ACG8Z3_002710"
     CDS             1108..2016
                     /locus_tag="ACG8Z3_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0824953.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2013..2555
                     /gene="sat4"
                     /locus_tag="ACG8Z3_002711"
     CDS             2013..2555
                     /gene="sat4"
                     /locus_tag="ACG8Z3_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFQ0824954.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2648..3442
                     /locus_tag="ACG8Z3_002712"
                     /note="aph(3')-IIIa"
     CDS             2648..3442
                     /locus_tag="ACG8Z3_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFQ0824955.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            3718..>4233
                     /locus_tag="ACG8Z3_002713"
     CDS             3718..>4233
                     /locus_tag="ACG8Z3_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0824956.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIE"
BASE COUNT         1447 a          635 c          969 g         1182 t
ORIGIN      
        1 ttttttatgt ttgaagcagg gttctcttta atgtttgtaa aagcaaattc gggaacagac
       61 gataaatatt atattgcggg tcatgttttt cgtatagttt catgtttaaa tcaagtgtta
      121 tttgcatgta ataatgctta ttgtatcaac gaaaagaaag ctataaaact gcttgaaact
      181 tttgaacata aacctgaaaa atataccgag aaggtaaatc atatttttga agtactcggt
      241 atctcacttt ttgaatgcta cgacatgacc gagaagcttt ataatgaagt gaatgaaatt
      301 gtatcggaga taaataactt tttaaacgag gagagttcag atgaaagaaa acaaatatga
      361 tgataatata ttttttcaaa aatacagtca aatgagtcgc tcgcagaaag gactggctgg
      421 tgcgggagaa tgggagactt tgaaaaagat gctacctgat tttaagggta agcgtgtgct
      481 tgatttagga tgcggctatg gatggcactg tatatatgcg atggaaaacg gtgcttcctc
      541 tgtagtaggt gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt
      601 tccacagatt gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt
      661 tgatgtaata ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa
      721 aaagatatat aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt
      781 ttttactgct catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt
      841 cccggtggac aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt
      901 tacaaaatat catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat
      961 aataaatcag attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc
     1021 ggatgaaatg cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata
     1081 tagaaaaaat aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta
     1141 ctttctttag cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca
     1201 aatattaata tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat
     1261 atagaaccgt ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg
     1321 caaaagccgg aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg
     1381 ctatttgatg attacaataa aattgatctt accttattgc ccttggaaga gttagataat
     1441 tacctaaagg gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg
     1501 gacatagttc cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat
     1561 gattgctgca atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag
     1621 gaaattttat ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg
     1681 atatcatgga aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag
     1741 tttattgaaa ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg
     1801 gattcctatg aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta
     1861 tccggtgagg tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca
     1921 aaatatacca gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca
     1981 tatgccgctg atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc
     2041 tgaaagatat cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
     2101 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
     2161 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
     2221 ctgataagat aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc
     2281 gaaaaaattg gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg
     2341 ggcaaggcat aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact
     2401 tgcatggact aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata
     2461 attgtggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
     2521 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
     2581 tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa
     2641 ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
     2701 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
     2761 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
     2821 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
     2881 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
     2941 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
     3001 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
     3061 ccgattagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
     3121 ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
     3181 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
     3241 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
     3301 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt
     3361 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
     3421 tttactggat gaattgtttt agtacctaga tttagatgtc taaaaagctt taactacaag
     3481 ctttttagac atctaatctt ttctgaagta catccgcaac tgtccatact ctgatgtttt
     3541 atatcttttc taaaagttcg ctagatagag ttctatatta gagatgtaaa gagttttggt
     3601 gaagaggttt gtgagtatgg cttttataac cagggtgcaa tgagaagcac taacagcagc
     3661 tagcttaaga acgcatagga tatacttttt atggaagtca cagaggagga ataaaatttg
     3721 aacaggatta acagagtaac ctctattctt attcagctgc aatcaaaaaa aataattcct
     3781 gccaaagaaa ttgcacagcg atttaatata agcttaagga cagtttacag agatatccgg
     3841 acacttgaag aagccggaat accaattgga tctgaggccg gaaaaggata ttttcttgta
     3901 gagggcttcc tacttccgcc ggtaatgttt acagcggcgg aagtgggtgc attgattaca
     3961 gcagggaaat ttttaaattg ccatggagat gaatcattta taaaggattt tgattcagct
     4021 atgtataaaa tcaaatctat tttgaagcat ggtgaaaaaa actatgcaca ggagttggag
     4081 aacagtatta acgtgtacag cacatctgga cagaaaaata cattggcgga taacgttatt
     4141 gcagcaattc agactgcaat ctgcaataaa agggtaatat caattcaata tcctgcatca
     4201 ggagggcaag aaccggaaag cagaatgata gag
//
LOCUS       DAZKSU010000130         3763 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000130 DAZKSU010000000
VERSION     DAZKSU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.137"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1013
                     /locus_tag="ACG8Z3_002714"
     CDS             <1..1013
                     /locus_tag="ACG8Z3_002714"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0824957.1"
                     /translation="LYIIIEGTLNRSYLIILFLFICFGISTSMFLPFLIDALKQLMTI
                     SLFICSFLIGKRFKMYSKNLTMIGWNFYFIGVVSMAFQVYLQRVYILSTGVIIGHYAE
                     MGLNRIAYAGLMGDFSFAGLYLATGCLYAFLKYVDLKNWKLLFFIIIEVFLLGAILCV
                     SARTGLVSLFVTMTLYYLFNINRISVPHLLVLIGGIITTPYILVMLMAGRSGQSLLDS
                     SGRLENYMSSLNAFSDKYLIGYGLGLNNLYTLLGVAVPHNFFIQYLLQTGIIGTLIIL
                     SGFFKFTYEELKKSGCYKWLFLMIVVGAMFIPDIVSSRFLYAIVVLCMISASERTIKK
                     ES"
     gene            1024..1485
                     /locus_tag="ACG8Z3_002715"
     CDS             1024..1485
                     /locus_tag="ACG8Z3_002715"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFQ0824958.1"
                     /translation="MRRKAEKWADKACRQYRNGQNKMSKISCYILRIIYGCEFYGQVD
                     FSSNAQLVHNGLGCVFHPKTKIGNNCKIYQNVTLGGNGKIINGKETVGAPTLEENVAV
                     FAGACVLGPINIGHDSIIGANAVVTKSVPPYSLVYGNPAIITELKFDYKFD"
     gene            1544..3001
                     /locus_tag="ACG8Z3_002716"
     CDS             1544..3001
                     /locus_tag="ACG8Z3_002716"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFQ0824959.1"
                     /translation="MGNNIHGKTAKALKWSSLAEIIAKIITPISNMILARLLTPDAFG
                     VVATVTMVISFADMFSDSGFQKFLVQHEFDNEEHMFSSTNVAFTSNLGISLLLWLFIC
                     VFNNKIAHLVGNSGLGLVIIVAGVSLPITSFSSIQSAIFRRNFDYKILCYARIISALI
                     PLVITVPLAIIGLSYWSLVIGSIIGNLVTAAILTKLSEWKPKLFFSKKEFYEMFSFSA
                     WSLVESLGTWLSSYIGTFIVGGILSVYYLGIYKTTMTTVNGIFAIVTSATTTVLFSTL
                     SRLQNSKTEYDKTYLDFIKIVSIFIIPLGIGIYVYRELVTNILLGSQWKEAVPFVGIY
                     GLMSCFTLVLGQYASEYYRGLGKPKANVLMTILHLAVLIPVLIIYSRKGFIELAYGRS
                     FVKIEQIIVFWIILWFGFKFNPFKILKQVWKSCASALLMGVLAKIMLEFCTNYVMQLL
                     SVCLCVILYFFIYSCIFKGKTEIINMVRMFTGKGY"
     gene            3036..>3763
                     /locus_tag="ACG8Z3_002717"
     CDS             3036..>3763
                     /locus_tag="ACG8Z3_002717"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0824960.1"
                     /translation="MKKVTILIPGMYYGGMERVAFITRQILLKNNYNVSFVTLFDNDA
                     DYQPNFEYLSLKCDIKPSKFGKVINIFKRVYKMKSVKKKLKTDITISFGQSCNLVNVL
                     SKRDDKVYVGIRSYDWLFQYIFHYYVDKFVYKKADAVVSVSQLIKRDAENIFNFNKDK
                     SYFLYNPYDLELINNKSKEKITEIEVPTDKKIIVTVGRLEDQKGLYHLIKAISMLKNK
                     DNVVVYILGHGTKKELLENLIKAY"
BASE COUNT         1301 a          412 c          679 g         1371 t
ORIGIN      
        1 tgttatatat tattatagaa ggaacattaa atcgttcata cttgattatt ttatttttat
       61 ttatttgttt tggaatatcg acgtctatgt tcttaccttt tttaatagat gcattaaagc
      121 aattaatgac tatttcgttg tttatctgta gttttttaat aggaaaacgt tttaaaatgt
      181 atagcaaaaa cttaactatg attgggtgga atttctattt cataggagta gtatctatgg
      241 cgtttcaggt atatttgcaa cgagtatata ttttatctac tggtgtaata ataggccatt
      301 atgcagaaat gggtttaaat agaattgcat atgcaggttt gatgggagat tttagttttg
      361 cagggttata tcttgctaca ggctgtctat atgcttttct aaaatatgtg gatttaaaaa
      421 attggaaatt attattcttt ataataatag aagtattttt attaggtgca attttatgtg
      481 ttagtgcaag aacagggtta gtttccttgt ttgtcacaat gacattgtat tatctattta
      541 atataaatag aattagtgtt cctcatcttt tagttctaat aggtggaatc ataactacac
      601 cttatatact agtaatgtta atggcaggtc gttctggaca aagtcttttg gattcttcag
      661 gcagattaga aaactatatg tcctctttaa atgcttttag tgataaatac ttaataggat
      721 atggattagg attaaataat ttatacacgc tactaggtgt agcagtacca cataattttt
      781 ttattcaata tttattacag acaggaatta ttggtacgct aattatttta agtggcttct
      841 ttaaatttac gtatgaagaa ttaaagaaat caggttgcta taaatggtta tttttgatga
      901 tagtagtggg agcaatgttt ataccggata ttgtatcttc gcgttttctt tacgcaatag
      961 ttgttttgtg tatgatttct gcatcagaaa gaactataaa aaaagaaagt tgaggtagaa
     1021 aaaatgagaa gaaaagcaga gaaatgggca gataaagctt gtagacaata caggaatggt
     1081 caaaataaaa tgtcaaaaat atcctgctat attttaagga ttatttatgg atgtgaattc
     1141 tacggacagg tagattttag ctctaatgct caactagtcc ataatggtct tggttgtgta
     1201 ttccacccta aaacaaaaat cggtaataat tgtaaaatat atcaaaatgt gactttgggt
     1261 ggaaatggca aaataataaa tggaaaagaa actgttgggg caccaacgct agaggaaaat
     1321 gtggctgttt tcgctggtgc atgtgtttta ggaccaatta atatcggaca tgattcaatc
     1381 ataggtgcaa atgctgtagt tacaaaaagt gttccaccat atagcttagt atatggaaat
     1441 ccagcaatca taacagagtt gaaatttgat tataaatttg attaaaaagt aatgtttaat
     1501 atgcgaaagg ttgctatatt tatttagcaa aatggggtaa taaatgggaa ataatattca
     1561 tggaaagaca gcaaaagctt taaaatggtc atccttagct gaaataattg caaagattat
     1621 aactccaatt tcgaatatga ttttagctag attattgact ccagatgctt ttggagtagt
     1681 agccactgta actatggtta ttagtttcgc tgatatgttt agtgattcag gatttcaaaa
     1741 atttttagta cagcatgaat ttgataatga agaacacatg ttttcatcaa caaatgtagc
     1801 ttttactagt aacttaggta tttctttatt attatggtta tttatttgtg tgtttaataa
     1861 taaaatagcg cacttagttg gaaattctgg acttggatta gtaattatag tagctggagt
     1921 ttctcttcca attacttctt tttccagtat acaatcggca atttttagac gaaattttga
     1981 ttacaaaatt ttgtgttatg ctagaataat atctgcattg atacctttag ttattacagt
     2041 accgttggcg ataattggac taagttattg gtcactagta attggatcta taataggaaa
     2101 tttagttaca gctgcaatat taactaagtt atcagagtgg aaaccaaaac tttttttttc
     2161 aaaaaaagaa ttttacgaaa tgttttcctt tagtgcgtgg tcactagtgg aatcattagg
     2221 tacatggttg tcatcttata taggtacttt tatcgtagga ggcattttaa gcgtatacta
     2281 tttgggaata tacaaaacta caatgacaac ggttaatggt atttttgcaa ttgtaacttc
     2341 agcaacaaca actgtattat tttcaacatt atctcgttta caaaatagta aaacggagta
     2401 tgataagaca tatttagatt ttattaaaat tgtttccatt tttattattc ctctgggtat
     2461 aggtatatat gtatataggg aactagtaac aaatattcta ctcgggagtc aatggaaaga
     2521 ggctgtacca tttgttggga tatatggatt aatgagctgt tttactcttg tcttgggaca
     2581 atatgcctct gagtactatc gtggtttagg aaaaccaaaa gcaaatgttc ttatgactat
     2641 cctacattta gcagtattaa ttccagttct aataatctat tcaaggaaag ggtttataga
     2701 actagcatat ggtagatctt tcgttaagat agagcagatt atagtctttt ggattatttt
     2761 atggtttggt tttaagttta atccatttaa aattttaaag caggtttgga aatcttgtgc
     2821 tagtgcattg ctaatgggag ttctagcaaa aattatgttg gaattctgta ctaattatgt
     2881 tatgcaatta ctttcagtgt gtctttgtgt aattctatat ttttttatat atagttgtat
     2941 atttaaagga aaaacagaaa taattaatat ggttagaatg tttactggta aaggatatta
     3001 agagagattt ttatggcaag gttgtgatta ggaaaatgaa aaaagtaaca attttaattc
     3061 ctggaatgta ttatggtgga atggaaagag tagcttttat aacgcgtcaa atacttttga
     3121 aaaataatta taatgtatca tttgtaactc tttttgataa cgatgcagat tatcagccaa
     3181 attttgaata tttatcatta aaatgtgata taaagccatc aaaatttggt aaagtaatta
     3241 acatttttaa aagagtttat aaaatgaaaa gtgtcaaaaa aaaattgaaa acggatataa
     3301 ctatatcttt tggacaaagt tgtaatttag taaatgtttt atcaaagaga gatgataaag
     3361 tatatgttgg aatccggtcc tatgactggc tatttcaata tatattccat tactatgtag
     3421 ataaatttgt atataagaaa gcagatgctg ttgtgtctgt cagtcagtta attaaacgtg
     3481 atgctgaaaa tattttcaat tttaataagg ataaaagcta ttttttatat aatccatatg
     3541 atttagagtt gataaataat aagagtaaag aaaaaattac agaaatagag gttccaacag
     3601 acaaaaaaat tatagtgacg gttggtcgat tagaagatca aaaaggttta tatcatctaa
     3661 ttaaagctat atctatgtta aagaacaaag ataatgttgt tgtgtacatc ttaggacatg
     3721 ggacaaaaaa agaattatta gaaaatttaa taaaagcata tca
//
LOCUS       DAZKSU010000131         3700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000131 DAZKSU010000000
VERSION     DAZKSU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.139"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..1894
                     /locus_tag="ACG8Z3_002718"
     CDS             179..1894
                     /locus_tag="ACG8Z3_002718"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824961.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1897..3660
                     /locus_tag="ACG8Z3_002719"
     CDS             1897..3660
                     /locus_tag="ACG8Z3_002719"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824962.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         1101 a          726 c          734 g         1139 t
ORIGIN      
        1 atgttttctc attttctttt ttttaaaaaa aatagtctat tcaatttcta aagcttcttc
       61 ataataaaac tttcatttat gaaaaggctt gttctctttt cattctaaat ttcctaagct
      121 acaatgtggt agtacttgtt ttttcaattg tggacaaata aataatagga ggaatcatat
      181 gattcagtta ttacgttatg caaaagacta tcgtaagcaa atcattttgg gcccgttctt
      241 caaatttctt gaagctgtct tcgaacttgt cctgccttta atgatggctt ccttgatcga
      301 taatggacta aaaatgaatg accgaggaaa aatcattgaa atgggcttat ggatggtcgc
      361 tatgtctgtg attggtctga tttgtgctat catttgccaa tactatgctt cagtagcttc
      421 acaaggattt ggtacagagt tacgtaatca gctaatcaaa aaaatcaata ctttctctca
      481 caacgagttg aatcattttg gaacagatac cttgatcacc cgaatgacaa acgacatcaa
      541 tcagcttcag ctagctttag caatggtcat tcgtttattg atcagagcac cgtttttaag
      601 tatcgggtca gtggtcatgg cttttgtgat cgactgggaa gtaggtctgt ttttcttagc
      661 attattgcct attttctcaa tcattttatt ttttatcatc aaaaaaaccg ttcctctcta
      721 ccaaaaagtg caagaaaaac tagatcagct caatgaacac gttagtcaga atcttagcgg
      781 tgttcgtgtc atccgcgcat ttgcgcagac aaaaaaagaa acagagaagt tcgataaaag
      841 cacaaatgat ctagcgaaca attatttacg tgtttccaat atctctgccc tattatcacc
      901 tgctacaacc ttgatcatga atgtcgggat catctgtttg ctgacagttg gcgggatcaa
      961 agtaaatatt ggtagcttgc aacaaggaca agttcttgct ttgatcaatt atatgaatca
     1021 aatgcttttg gcactgatcg ttgtttcaaa cctcgtcgtg atcttcacac gtgccgaagc
     1081 ttcaggaaat cgggtgaaag aggtgctaga cacagaaaat tcgattcttg aagctgaggt
     1141 cagtactact cccgattttt cttctgaagc aattatcgct tttgaccatg ttgacttccg
     1201 ttatactcct gattctggtc tttctctcca aaatattaca tttcagctga agcgaaatac
     1261 agtccttgga atcattggtc caaccggaag cgggaaaacg actttgacac aattgattcc
     1321 gcgtttctat gatgtcagtt ccggtacagt tataatggac ggaatggatg ttcgttcttg
     1381 gcagctggat catctccgtc agcaaattgc tcttgttccc caaacttctg tactatttac
     1441 cgggactatt cgtgaaaacc ttcaatgggg taagaaagac gccacagaaa aagaatgttg
     1501 ggaagctctg aaaatcgctc aggcagaaga atttgtccgt cagcttccaa atggtttaga
     1561 tacaaaagtc atggaaaatg ggaaaaattt ctctggcgga caaaaacagc gactgacgat
     1621 tgcaagagct ttgattgcca agccagcatt actgatttta gatgactctt taagtgcgtt
     1681 ggattatcaa acagacttgg atttgcgtaa ggcgctacag acttatcttg gcagtacagt
     1741 gattattatc tcccaacgtg tccgctctat tcaagaagca catcatatct tagtgatgga
     1801 tcaaggtaag attgctgctc agggtaccca tgaagaatta cttgctcaat cagcagaata
     1861 tcaggaaatc gtcgcatcac aggaggaaga atagttatga aagtaaaaaa attatccttg
     1921 cagacgctcc gccgattcat tccttacctt actgcctatg caaaagacat ttggctagct
     1981 gtgatactag gaatcatcaa tgggattgca acagttgtga tgacgttaca aattggtcaa
     2041 agtatcgatc aaatgattgg agaaggacag gtccgcttca atcgactgtt ccatttactt
     2101 attctattcg gtggtatcgt tttaatcaac gtagtcagtc aatggctcat ccaagtgtta
     2161 agcaatcggt tagcttactt atccgttgca aaattaagaa aagatgcatt tgctcatctg
     2221 aatcgattgc cacttcgtta ttatgatcaa aactcccacg gaaatatcgt cagtcgattt
     2281 acaaatgata tggacaatat ttccattgca atttctgcgg ccttcaatca attattttct
     2341 ggagtggcaa tcatcattct ttcgtttatt tttatgatgc gtctaagtcc acttctgaca
     2401 ttggtcgttc tttgttctac accaattatt ttccttgtga gttggatcgt tgctcgtgct
     2461 tcacaaacaa atttcgatag acagcaaagg attattgggg atatctcggg ctttgtgacc
     2521 gaacgaatcg gtaaccagaa aatcgtgaaa gcttttcagc aagaagaagc tagtcaacag
     2581 caatttgaac tgctaaatgc tgatctatat gaaaaaggac aacgggcaca attttcttct
     2641 tccttgacaa acccgtcttc tcgttttgta gatcaccttg cttatttatc gattggttta
     2701 gttggcggcc tattggcatt gaagagtggt tcgtcaatca ctgttggtgt gatttccagc
     2761 tttacgatct actcaagtca gttttctaaa ccatttatcg aactttctgg aatcacgaca
     2821 caactgcaaa ccgctttttc tggtctagaa cgagcatttg atttgatcga tcagccagaa
     2881 gaaaagcctg atttgccaga tgcctatgcc ttggaaccca aaaatatcaa aggaaaagtc
     2941 tcttttgatc atgtttcttt ttcttatgac cccaagcagc gtttgatcga agatttcaat
     3001 tttactgctt atccaggtca aaccattgct atagtaggaa aaacaggggc cggaaaatcc
     3061 acacttgtta atttgctgat gcgtttttat gacacaacaa aaggaacaat tacagtcgat
     3121 gaccatgaca tccgctctat ccaacgtgat acattacgta aaagttttgg aatggtgtta
     3181 caagatactt ggctatttga cagtacattg cgagaaaatc ttcgttttgg gaatccggaa
     3241 gcaagtgacg cagaaatcaa tgatgcccta aaagcctctt atatgtatga atttgtagaa
     3301 cgtttgccgg aaaaattaga tactaagatt ggcggtcaag ggttaaagat atcagatgga
     3361 caacgccaac tgttgacgat tgctcgaacg atgatcagta atccccctat gttgatctta
     3421 gatgaagcta ccagctcagt cgatacattg acagagaaaa aaatccaaca agccttctta
     3481 gcaatgatgg aaggaaaaac cagttttgtg attgcccatc gcctgtccac aatcaaaaat
     3541 gcggaccaga tattagtgat ggatcaggga gctattgtcg aaatgggcac ccatgatgag
     3601 ctgcttcaaa aagatggtta ttaccgacgg ctctatcaag cacaattcac agcacaataa
     3661 aaacaagcag tcttctggga tgggaagact gcttgttttt
//
LOCUS       DAZKSU010000132         3641 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000132 DAZKSU010000000
VERSION     DAZKSU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.140"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..586)
                     /locus_tag="ACG8Z3_002720"
     CDS             complement(<1..586)
                     /locus_tag="ACG8Z3_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824963.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKIND"
     gene            complement(1224..1481)
                     /locus_tag="ACG8Z3_002721"
     CDS             complement(1224..1481)
                     /locus_tag="ACG8Z3_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0824964.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1474..1743)
                     /locus_tag="ACG8Z3_002722"
     CDS             complement(1474..1743)
                     /locus_tag="ACG8Z3_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0824965.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(1874..2053)
                     /locus_tag="ACG8Z3_002723"
     CDS             complement(1874..2053)
                     /locus_tag="ACG8Z3_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824966.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            2151..2387
                     /locus_tag="ACG8Z3_002724"
     CDS             2151..2387
                     /locus_tag="ACG8Z3_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824967.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            complement(2434..3000)
                     /locus_tag="ACG8Z3_002725"
                     /pseudo
     CDS             complement(2434..3000)
                     /locus_tag="ACG8Z3_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3076..>3641
                     /locus_tag="ACG8Z3_002726"
     CDS             3076..>3641
                     /locus_tag="ACG8Z3_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFQ0824968.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT         1143 a          669 c          522 g         1307 t
ORIGIN      
        1 tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa cattcaaaac
       61 attcttctat agttggttta cttccttgag gagaatgaac caatttatta cgtttttcta
      121 tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag taccctttaa
      181 acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat attagagtta
      241 aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct gtaattatcg
      301 aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt ataagagaaa
      361 aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca gaagttatat
      421 ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa atttctatcc
      481 aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca gcaagcatcg
      541 ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca gcaatattgc
      601 taaaatcagc taatttttct ttagaataaa aaaaaacatt tatatcagaa tcaataaatt
      661 ctattttttt attactatat cgtcatcatt atcttgacca tttatgttta ttgttccctg
      721 agtggggagt acttctttca aattcaagtt aaatttatat tccattactt ctcctccaat
      781 atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt aatttttagc
      841 taaattctat gagaagttta tttttgttgt ttttgctaat taactaaaac tagtacaaaa
      901 aatttaaaat agattattaa acctattgct tattctattc agtcatatat ataagcgtca
      961 tcctttagta actaacatcc cacactacaa tagtattata acatatctat tcaacttcaa
     1021 attgttgaaa tttaagccat ttatatttac ttttcctcaa ctttagaaat gattttacta
     1081 accttatatc cgtaatacgc gtaacaaaca cattcattaa taacaatcaa cactaagcta
     1141 tattagcttt ttaaatgact agggtaaata aaataaggat caaaaacttt taagtttctg
     1201 accctttcct tacttccgat tggttaatag tgatcttttg cagaataaat aaatatcgtt
     1261 tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga ccattttcca
     1321 gataaatcat gctttaatgg ctcaggtttt cctaatccag caaaggggga acgatcgata
     1381 tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc atgccaataa
     1441 agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa cctcgattaa
     1501 gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa ttttttccat
     1561 gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat ctctttttga
     1621 taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt cagcatcctc
     1681 attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag ctactgcttc
     1741 cattcctttc accccttatt tatttctata aaacaattgt acattatatt gtacaattaa
     1801 gcaaatgatt aatattctaa cttcttataa ttaaaagtgc tatcactttt agtttttagg
     1861 ataaaaagtg acgtcacttt ttgagtgctg gtacctcaaa tcctgctttt tcttgggcaa
     1921 tcaataaatc taacgcaata cacataatcc tggattcact tttgccgtac ttctgcttta
     1981 gttgctgcaa ttctttttgt ctcttttcat ccaacgtaac gggtaaccgt ttatttccta
     2041 ttgcaattgg cattagtact ctctcctttc atcgaaataa ccctatgaaa aatatggctg
     2101 caatcatctt gaatagtaga ctcacaaggg ctaatacaaa gcgccaaaag atcaaacaaa
     2161 ataccagtat tgccaaaaat actacgaagt ttaaatgcac agctgcagca tggcgcataa
     2221 gtatttcgcc aacaaatagg actaacaata gaggaatgat tttattcata tggatctcct
     2281 tctttctctt aggttatcgg gtgttgcccg cttatttcgc accacctaag cggcgggggc
     2341 ggttggagcg attggtgcga aataagaacc attcccttag ttactaggtt ttttgtcctt
     2401 atttcacaca ttctcacttt tcggtataaa tgattattgc ctttttttcc tttcaaatcg
     2461 ttgatatggc attcgtttac ggtcaagttc attttggatt tcttctgtat cgtcgtaagt
     2521 ataggtgtct aaaaaatcag ttaaagtcgt gtaccagtcg tcgcctgggt tatctacttc
     2581 ttctacatat tccctcaaaa atgcttcggg tacagcgagt tcataatcca ttcccattaa
     2641 cacgatagat tccgttccac acgtcaaaaa tctagtaaat acaaggtaat aatcgtctat
     2701 cgtcgcatct ttgctgttta gctcttccat ttcttcttcc attaattcca tgctctcaaa
     2761 aattgaataa tattctgatt ctgagtaata gtgtttcctg caaatcgtgt agctgacaaa
     2821 gttttcgacc agctcactcc tttctaggta aacattcatg atgtattttt ctgtttctag
     2881 tcgtagaagc tccgttcctt tttttaccgt ttcaggacga cgtttataca cgtctaaacg
     2941 attatggctg actgtgacct tcgtttgact cgtttcactt tccaaataca attgttcttc
     3001 gggttctgtt gcaaagtttt aaatctacta tcaaataagg tagaataata gaaaaagata
     3061 gcaggaggaa tgacgatgaa tcattttaaa ggaaagcaat ttcagcagga tgtgattatt
     3121 gtagccgtgg gctactatct tcgttataac cttagctatc gtgaagttca agaaatctta
     3181 tatgatcgtg gcattaacgt ttctcatacg acgatttatc gttgggtgca agaatatggc
     3241 aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg
     3301 gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca
     3361 gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct
     3421 tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc
     3481 ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa
     3541 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
     3601 cgcaataaat tctatcgaag tttacgcact gcctctacca c
//
LOCUS       DAZKSU010000133         3479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000133 DAZKSU010000000
VERSION     DAZKSU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.141"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..203
                     /locus_tag="ACG8Z3_002727"
     CDS             <1..203
                     /locus_tag="ACG8Z3_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0824969.1"
                     /translation="PLNENSNGILRRNGLPKSMDFREVNQTFISSVSNQRNHIPRKSL
                     NYRTPIEIFLSYVQEAFYSSLI"
     gene            697..984
                     /locus_tag="ACG8Z3_002728"
     CDS             697..984
                     /locus_tag="ACG8Z3_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824970.1"
                     /translation="MEIKEIKVRGINKKYVQEIDHRCVELTEQTGQKWSRNDYLKLLI
                     ENDFERPLMDYKKDQFDRLLEKFTDVQLHNTKVLEAYTNEVKNLIEILIAH"
     gene            1002..1424
                     /locus_tag="ACG8Z3_002729"
     CDS             1002..1424
                     /locus_tag="ACG8Z3_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824971.1"
                     /translation="MVKAIEKHITFYEAHDEVLKHIMETQPTVKNYSEAIRFLCMNYD
                     PEIEKNNNMTNAKLNAMGKEISILIEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
     gene            1444..3036
                     /gene="mobP2"
                     /locus_tag="ACG8Z3_002730"
     CDS             1444..3036
                     /gene="mobP2"
                     /locus_tag="ACG8Z3_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0824972.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQNSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQIEQERQ"
     gene            3292..>3479
                     /locus_tag="ACG8Z3_002731"
     CDS             3292..>3479
                     /locus_tag="ACG8Z3_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824973.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         1386 a          537 c          664 g          892 t
ORIGIN      
        1 gcccattaaa cgagaattct aacgggattc tgcgtcgtaa tggactgccg aaatcaatgg
       61 attttagaga agtgaatcag acatttattt ccagtgtcag caatcaacgt aatcatattc
      121 caagaaaatc attgaattac agaacaccaa tcgaaatatt tttgagctat gtacaagaag
      181 cattttattc tagcttaatt tgacaaatca tatgaaaaaa gtatataaaa aatgtttatg
      241 gatcagctat atcaccctag gtatagctgt taaagagatg ggagaaaaca ttaaccacga
      301 ttctaacaca aacatcaagc acataaaaca tttaacactc ctcctttctg gctgaagatt
      361 tcaaccaaac aggaagacag cgtatcaatt atagcataca ctttttctta aattcatttt
      421 tcatatgaca aaatatttcc ttctaatcga caaagttaga aggatttttt attgtctaaa
      481 tacacaaata accttaattt ttaaagagca taaggaaacg tgggaacact ttttaaaaaa
      541 agtgttccaa taggctgtgc ctattacgtt tctctaaaac aaaagtgggt acaaaatatt
      601 cgatttatgg atagtgtatg gatagtaaaa cagctataca tggatagcat atggatagac
      661 tccatagact atccatactt ttttcggagg gtgaaaatgg aaattaaaga gattaaagta
      721 agaggtatta ataaaaaata tgttcaagaa attgatcata gatgcgtaga attaacagaa
      781 caaactggtc aaaaatggag tcgaaatgat tatctaaaac tattgattga aaacgacttt
      841 gaacgtcctt taatggacta caagaaagat caatttgatc gtttattgga aaaatttaca
      901 gatgttcagc tgcataatac aaaagttttg gaagcataca cgaacgaggt caagaactta
      961 attgaaatat taatcgcaca ttaggaagga gataatgttc tatggttaaa gcgatagaaa
     1021 agcatattac attttatgaa gcgcacgatg aagttttaaa acatattatg gaaactcagc
     1081 ctacggtaaa aaactattct gaagccattc gatttttatg tatgaattac gatccagaaa
     1141 ttgaaaaaaa taataatatg acgaacgcaa agttgaacgc aatgggaaag gaaatttcta
     1201 ttttaataga aatggtttca gagtttgcgg atcttcattt gaaagataca ggaatattaa
     1261 ttggtacaaa ttcaactgtc tatcaagaag caaaagagcg tgtggaaggg aaaataaaag
     1321 cgaatcaaac gaagaagtat tctcctaaaa tcaagaaatc aataaatgaa tctgttcatg
     1381 ccagagtaga cccgtttgat ccaacaaagt ttttaaaaag ataaaaagta gaaggggaat
     1441 atgatgaata aatcgcctgc agtcattctc acttgtcaat ttaccttgcc caataaaaaa
     1501 tctttttcaa catacattga ttatatgaca agagaaaaag cgctggaaga aatggaacga
     1561 cgtacgcctg aagaagaaca ggaactgcaa cgaatcaaga atgctttaga ggatttttat
     1621 attcctgaag gagaaacttt ttcggagagt aagacggata aagaattcag cgaaacgtcg
     1681 aatgaagctc aatctttgat gggaaatgga atcaactttg acaagttaga ggaacaagat
     1741 tttaccaaat atctttctta tatgacccgt cactatgcgt tagaacaaaa gaaagaactg
     1801 acgcaaagtg aacaaaaaga ataccaacgc ttgaaccaag caataaaaaa atatcaaaca
     1861 gagccacaca aaaaagcttc aggaaacctt cctggagttt tttctatggc ttcagacgaa
     1921 gtgaaaggaa gtgatctgaa ggagattaag caaatcttcc aaagaggaca acaaaaaggc
     1981 tcaatcattt atcaagatgt tgtctctcat gataattcgt atcttgaaaa aataggattg
     2041 tacgatccca aaacagatac tttagatgaa gaaggattaa aagcagctgg aagaaaaatg
     2101 atggagacat tattcgagaa agagcaactg aacgaaacag gctactggat ggcaacgatt
     2161 caccgtaata ccaaacatat ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt
     2221 aaaccttaca cggaggttga aaatggtgtg agttatatcg taccgaaagg taagagaaga
     2281 caagcaacat tagacgcaat gaagaccaat tatgtacatg agttagaacg tttttcttat
     2341 gaaaaggatc gaatgattgg tatggagaaa cgaaatttat tgacgcggaa aagtgatttg
     2401 cgtaacacct taacaaaagt ggtaaaagag ccagcgcgtt acgatcgaca cgcaatgaac
     2461 ttattaaatg aagtatataa aaccctacct gacaaacgtt cggaatggca ttatggggat
     2521 gaaaaacgaa ccaaactttc accatacaca agagagaaac tcgatgagtt aaccaaatat
     2581 gttttaacaa aagaacctga atatcaagaa tatatacaga tttcaaaagc attgaaagaa
     2641 gaagcaaaaa gtctttatgg ggaaagcaaa agagaatcaa aggatgcaga aaaaaatgct
     2701 ctctttgatt tgaaaaagcg cacaggaaat gccattctaa cagaactgaa gaaacagaat
     2761 agtcaaataa aacctttaat agaaagtatg gatcagatgc aatctattta caatgaacga
     2821 tcctttcgaa cgcttaaaca gttaccaacg aatgaataca aatatagtta cgaagaattc
     2881 atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc aacggagttt gcgtcaatta
     2941 aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa gtaaagccta cgaagagcaa
     3001 caacgaagaa tccaaataga acaagaaaga cagtaaagac agtaaagtta gcgccttatt
     3061 atactacttg ggaaatgaat gaagagggga tcaataactt cccgtgtaaa agcattgaag
     3121 agtctatttc actcacagaa tgttagttgt taagtgaata aaagaagtaa aagagtcctg
     3181 aacgtaattg tccaggactc tttttttcaa tgaattgttt tccgccaatt caatgagtgg
     3241 tgcgatttgc gactacatta tatcaaaaga aagagaaggg aggaagaaga aatggcttat
     3301 tcaacgattg gagttgttgt tgaaaaaagc agagacaatc tcgtttttgt gacagaaatt
     3361 caaacaggca gagcttttgt cgtcacagac aaagcggcaa aagcgtatca aaatggcgat
     3421 attttaacgt taaatctgac tacgaagacg ttcgtcgatg cggcagaaga ttatccatt
//
LOCUS       DAZKSU010000134         3459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000134 DAZKSU010000000
VERSION     DAZKSU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.142"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..793)
                     /locus_tag="ACG8Z3_002732"
     CDS             complement(<1..793)
                     /locus_tag="ACG8Z3_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFQ0824974.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFT"
     gene            complement(900..2390)
                     /locus_tag="ACG8Z3_002733"
     CDS             complement(900..2390)
                     /locus_tag="ACG8Z3_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0824975.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KKSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKLVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREALGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALSEFFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(2785..3024)
                     /locus_tag="ACG8Z3_002734"
     CDS             complement(2785..3024)
                     /locus_tag="ACG8Z3_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824976.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSLIESGKSDPTL
                     KTLERIAELTNSTLVIDLIPNELEQVELQIEEEKQ"
BASE COUNT          992 a          635 c          558 g         1274 t
ORIGIN      
        1 tggtaaagat atttcttagg aagtgaactt ggcatctttg ccaacttacg ttggtgaagg
       61 attttctaat ggcagagact aatcctttgt gcgcatcaga aataacgagt tccgtacctt
      121 gtaaaccgcg ttcttttagg tattcaaaaa atgttgtcca ggtctcttcg ctttcgccac
      181 tttgaatcat gaagccgata atttcacggt cgccatcttt ggttattcca atcgctatat
      241 gacagctttt tgagagtact cgattttctt ctcgtacttt tatatagagt acatcggtca
      301 ttaagtaagg ataatttttt tctgataata aacgattctg ccactcgtta accataggtt
      361 ctagctgttc tgttaagcta gaaacgaagg acttagagac ggatttacca caaagttctt
      421 ccacaatttt tgatacttta cgagttgaaa cgcctgatac atacatttcc aacattgaag
      481 ccatgagggc tttttcgttt cgttgataac gttcaaacac tgtgggtgaa aaatggccat
      541 cacgtgttct gggtactttt aattctagcg tgcctacacg tgtcgtaaag ctgcgctcat
      601 aatagccatt tcgttgactt tgtcggtttt ctgttcgttc atattctttt gcttgaatat
      661 attctgttcg ttgattttcc attagttgat taaataccgt tgttaaaata tttttagaaa
      721 cgtcatcctt tacagaatat tcaataatgc tttgaatctc ttcgcttttc agtgtaaaat
      781 gtacttgggt catgtaaaag tcctcctggg tatgtttttg tcgttaaaaa cattgtaccg
      841 taaaaggact gttatatggc ctttttactt ttacacaatt atacggactt tatctatttt
      901 cagcctgtat catagctaaa caaatcgagt tgtgtgtccg tttttgggcg ttctgctagc
      961 ttgtttaaag tctcttgaat gaatgtatgt tctaagtcaa aaaactctga cagcgccttt
     1021 atatagcttt ctttttcttc tttttttact ttaatgatcg atagcaacaa tgatttaaca
     1081 ctagcaagtt gaatgccacc atttcttcct ggtttaatct taaagaaaat ttcctgattc
     1141 gccttcagta ccttcagcaa tttatctaat gtccgctcag gaatgcctag cgcttctcta
     1201 atctcttttt tagtcgtcac caaataaggc ttgtatacat cacttttttc gctaatataa
     1261 gccattaaat cttctttcca ttctgacaaa tgaacacgtt gacgttcact tctttttttc
     1321 ttgaatttaa accacccttg acggacaaat aaatctttac tggttaaatc acttgatacc
     1381 caagctttgc aaagaatggt aatgtattcc ctattagccc cttgatagtt ttctgaatag
     1441 gcacttctaa caagtttaat cacttctttt tcttctaagg gttgatctaa tcgattatta
     1501 aactcaaaca tattatattc gcacgtttcg attgaatagc ctgaactaaa gtaggctaaa
     1561 gagagggtaa acatgacgtt attacgccct attaaaccct tttctcctga aaatttcgtt
     1621 tcgtgcaata agagattaaa ccagggttca tctacttgtt ttttgccttc tgtaccgctt
     1681 aaaaccgtta gacttgaacg agtaaagccc ttattatctg tttgtttgaa agaccaatct
     1741 tgccattctt tgaaagaata acggtaattg ggatcaaaaa attctacatt gtccgttctt
     1801 ggtatacgag caatcccaaa atgattgcac gttagatcaa ctggcaaaga ctttccaaaa
     1861 tattctcgga tattttgcga gattattttg gctgctttga cagatttaaa ttctgatttt
     1921 gaagtcacat aaactggcgt ttctaaaaca aaatatgctt gataaccttt atcagatttg
     1981 ataattaacg taggcataaa acctaaatca atagctgttg ttaaaatatc gcttgctgaa
     2041 atagtttctt ttgccgtgtg aatatcaaaa tcaataaaga aggtattgat ttgtcttaaa
     2101 ttgttttcag aatgtccttt cgtgtatgaa cggttttcgt ctgcatacgt accataacga
     2161 taaacgtttg gtgtccaatg cgtaaatgta tcttgatttt cgtgaatcgc ttcttcggaa
     2221 gtcagaacaa cgccacgtcc gccaatcatg cttttttttg agcgatacgc aaaaatagcc
     2281 cctttgcttt tacctggctt ggtagtgatt gagcgaattt tactattttt aaatttgtac
     2341 tttaacaagc cgtcatgaag cacagtttct acaacaaaag ggatattcat tcagctgttc
     2401 tcctttctta cgaaaattaa ttagttagaa gctacgatca aagttgaatc acaacaaaaa
     2461 aggcaatcaa ctaagttttt cttaattgat tgcctggtat cttcttaaag acttgaaatt
     2521 ccctcaaaaa cccgatataa tgggtttaca gatatttaag tatctgatta ataaagtaat
     2581 taaatacttt accaaatttc gggtctcgac ttctttaatt gattggtggt aatcaattaa
     2641 ggctcgcaac ttattttctt ggcggaaaat aagttggtcg tggctctttt tttgtattct
     2701 ttattcagtt cgttgtttcg ttatatctag tataccgctt ttaaaaaaaa taagcaacaa
     2761 tttcgttaaa tatattaacg acaatcattg cttctcttct tctatttgca actctacttg
     2821 ttctaattca ttaggaatta agtcaataac taaggttgag tttgtcaatt ctgcaattcg
     2881 ttccaatgtt tttaatgtcg gatctgattt tcctgattca atcaatgaat aatttgatct
     2941 actcatttct aattcttggg agaatttttc ttttgatttg gtacctctca tcttttttaa
     3001 gctttctcta aatgctagtt ccaagtttct ccctcctgtt tatgtaagta ttttgcacgt
     3061 tttccattta attatatcgt tacaccttgt aaatcacaag tgattaatca caaatcactt
     3121 gtgattagtg attgttattg gtgttgccta gaacgattct aagcacatgt ttaattttaa
     3181 agtgggcaat tatctttaaa atgtcctaca atgattcaat aaaagcatgg ctgctagtga
     3241 aacttgcgac catgctttta aaaatactta atggggtccc gagcgcttta gctccttgga
     3301 agctgtcagt agtataccta ataatttatc tacattccct ttagtaacgt gtaactttcc
     3361 aaatttacaa aagcgactca tagaattatt tcctcccgtt aaataataga taactattaa
     3421 aaatagacaa tacttgctca taagtaacgg tacttaaat
//
LOCUS       DAZKSU010000135         3457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000135 DAZKSU010000000
VERSION     DAZKSU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.143"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2177)
                     /locus_tag="ACG8Z3_002735"
     CDS             complement(<1..2177)
                     /locus_tag="ACG8Z3_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0824977.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
     gene            complement(2849..3117)
                     /locus_tag="ACG8Z3_002736"
                     /pseudo
     CDS             complement(2849..3117)
                     /locus_tag="ACG8Z3_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          983 a          763 c          539 g         1172 t
ORIGIN      
        1 aggacttcac cactgccttc agcatgttta attagttcaa caccacccgt atcaaggatg
       61 ttttcaatgg ttaacgtcac ttcttctatg gagtattcat ttataaaaac atcatgtacc
      121 gtcgcatcta aaagataccc ttccggtgct tctgtttctc ggacttgata gctacctaca
      181 ggaagcggtt cacttaaaaa ttctccgttt tctgccgtta taccttcttg tatcacttgt
      241 gaatcgcttg ataaaatttc aaattttgct cctgccagcc tttggccatt ctcatcagtt
      301 ttaattactt taatacgccc atcgattttc cttgttctta cagtactcgt attcaatcga
      361 ttgtcactat tcacaagcgt tgcaaagttt aaaatttgat cgccgaccgc atacgcctct
      421 acattgacct taaaagagag ggtaaaactt gtcggaccac caatcgtatt attcgcactg
      481 aaacgaacta ttccattatt gacttcggca tcaatgaaag agggaatatc tgatagaaat
      541 gacacaaatt ccatgccttc ttccaaaaca tcttcaataa caagcgtatt tcttgttcca
      601 gaaaacatcg agacatgcag agtatacgtt agttcatttt cattgagatt gataacattt
      661 ttgtccacac tttttcttaa agagattgcc ggaagattac tttcagggat tctcaagcgg
      721 tagtctcggc tcattacctg attattgtag gtcatactcg ctcgattccg agaaaaccca
      781 tcctcttgtc ttccttcatt gaacaagtcg acatcggtca cttcaagagc atattcaata
      841 aaatacgatt gaccctctgc tagattacca aagtccacaa caagtgtatt cgtttcctta
      901 ttaaaagata ctttgtccac aaattcctcg gaaacataat gagttctaac gtttgcttca
      961 cctggattcg ttacttctgc gatccgaacc ccacctgaaa taggttctct atttccagtg
     1021 gaactccatg tagtcggatt cggtgccaaa gttgttcctt gcggtagtac atcggtaacc
     1081 actacaccgt cttttcgtga tccttcataa tttacctgca aaataaattt attactgttg
     1141 gtcacgtctt gcccgtcaat cagcagattc ccaagatttc cttggctgtc gatttctcta
     1201 gagccatagt ataatttcga aaaaatgggt cttgatgaag gaataggcgt aattgaaaat
     1261 tccgtgtttg ctgctcgctc agcataagta agactcactt gaaagtcatt atttggctca
     1321 gtaccgcctg attgaaagaa agcaacttca aacgtgatac tgaatgtata cgactgagcg
     1381 gcttcattta atccttcata atcaaaggta aatggaaaaa caacgtctgt gccttcttgg
     1441 taaaatgcca gtggctttaa aggtgaatca tcagcgacct ccactctaat gaactcaatt
     1501 aagccaatcg atgctctagg aactcgtacg accatctccc catattcttc aagaatatcg
     1561 ccagcgcctc cttgaatgta tccctttaac gtttgtgtat gcctgctcgg caaagtgggg
     1621 cgctcagcag agatgccgat gtcataattc aagagctcct cttgtctttc aatctgagat
     1681 tcctgcgtta tagatggctc agatgatctg gcttcattaa aagattcact taagctttct
     1741 tctggtatat taaaagattc acttgtgctt tcttctggta cattaaaaga ctcacttgtg
     1801 ctttcttctg gtacactgat ttctattgag accgtatttg attgaatatc accgcttctt
     1861 gcggtcaatt cgtatttacc tgaatgctgg gaagaataag taatcagacg ttcgtaatca
     1921 ttgagttgct tcacttcccc ttcaatcaaa tcctcactct tatcaatgac caatgcattt
     1981 agcaggtctg gtgttcctct gactcgcaac tggaactgct cacccaaagc agcctcctct
     2041 tgatcaactt cgatcgtcag cgaacgctct ggctgttctg ctaaagactc agctagaacc
     2101 gttatgttcc caaattgatt gatcacaata agcataacta gaaacatact taacagttta
     2161 tgaaatttgt tcctcactat tactccccct tataattttg actaaacaac aaggtacccg
     2221 ataattttct aaaaagattc cccaactcaa aatggctgcg ttacctccct cgtattttcg
     2281 gttcaactca agcgttgcaa tgtaagctgt aaacaaagct aatgattgcc gattgtcttc
     2341 tgttgattga ttcgatagct ttgtatgact tttagttgtc cgtgtggctt aaccataatt
     2401 atgatttcac gattcctcct gccagtgcct tgcacaagct ggagcgtaac cctaatagtt
     2461 attttgttaa tgtacttata tacttttata cttttccatt agaaacaatc actaaatcgt
     2521 tgataccaag gtcagactct agaatttcat cagtgagtcc ctgtattggt taaccttcat
     2581 caatcccaaa aaaatatatc aatgcttagc atcaaaatga atatatagtt tagaatactt
     2641 gggattcatc gataactgat cgcttatttg gccatattat ttggcacttg gttcaactaa
     2701 acgcatttag gatgttcctt cattctcgtc atggatgttc tcaaaccgtc attactaata
     2761 aactgtccac cgaatcgatg gttcatttct tgcattttcc acgtccaatt actctttttg
     2821 ctctttattt gggcgtagtt tcactttctt aacttgtatc gtcttccatc aactccttaa
     2881 tcatcttctt agctttattg gctcggtttc cttgtaaaag gtatctacaa atagtgacaa
     2941 tctgtattaa atcttcatca ttttctgtaa tgtcaataat ttctatattc tgtaccatat
     3001 attgcaaagg gtttcactaa gttcataacc gaagcgaatt aaacaacttt tgtatagaat
     3061 aactaccttt tctatttttc cattggtaat tctatctatt aattggttta atccttttgt
     3121 tgtagttgat tccactacca agatgattct ctcgaatttt aaagcttgaa gatgtattgc
     3181 tatttttgct aactggcgta tttgcaacta tttttccctt ctattcttcc ttcattgatt
     3241 cccctctaaa attactatcc ataaaaaatt gcataaatga caatagtatt tatattagtc
     3301 atactgcttt ttaacacagc aaattccaga aaaacattcg ttacatattt gtgaattttt
     3361 ccaaaaaata acatttttat aaaacgacga aaaataaatc actaccagtc aacttctata
     3421 atattaccaa ataacaaact aactatccat caataac
//
LOCUS       DAZKSU010000136         3401 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000136 DAZKSU010000000
VERSION     DAZKSU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.144"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1072)
                     /locus_tag="ACG8Z3_002737"
     CDS             complement(74..1072)
                     /locus_tag="ACG8Z3_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0824978.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1147..1443)
                     /locus_tag="ACG8Z3_002738"
     CDS             complement(1147..1443)
                     /locus_tag="ACG8Z3_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0824979.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1436..1729)
                     /locus_tag="ACG8Z3_002739"
     CDS             complement(1436..1729)
                     /locus_tag="ACG8Z3_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFQ0824980.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1875..2426)
                     /locus_tag="ACG8Z3_002740"
     CDS             complement(1875..2426)
                     /locus_tag="ACG8Z3_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFQ0824981.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2600..2899)
                     /locus_tag="ACG8Z3_002741"
     CDS             complement(2600..2899)
                     /locus_tag="ACG8Z3_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFQ0824982.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2926..3201)
                     /locus_tag="ACG8Z3_002742"
                     /pseudo
     CDS             complement(2926..3201)
                     /locus_tag="ACG8Z3_002742"
                     /inference="COORDINATES: protein motif:HMM:NF017894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
     gene            complement(3214..>3401)
                     /locus_tag="ACG8Z3_002743"
     CDS             complement(3214..>3401)
                     /locus_tag="ACG8Z3_002743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824983.1"
                     /translation="LEKLTKQRDMKNFNNCVVLIIITELTLFTNIYYIHEAQSSLIED
                     WAIFMAKEGKEMFQNKG"
BASE COUNT          993 a          668 c          461 g         1279 t
ORIGIN      
        1 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
       61 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
      121 ttcttccatc tgtataaaca cgaaattgtt ttccaccagc catggtcgtg tttccatcat
      181 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
      241 tatattcatt tgtgctaatt aaattagtat ttgatacatt tttatcagga gaatcataca
      301 accataaagc tttattattt agcacagatt ctttagcagc ttcccaactt gaaattcctg
      361 aactacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
      421 gtgtttgagg agtttgtcca ttcacaatat cactgaaacc ttgttttaac tgttgatttt
      481 gagtttcgtt aaagtacaga taattatttt cattaccttg attttgttgc gttaaataaa
      541 ctttaggttg tccattttga aaaccgaaaa agtaaacatg ctgccctaaa tattctccag
      601 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
      661 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatcgcca ttaatcacgg
      721 cttgtggtaa aggagtgcca tacagactta cattattctg aagattgtag cttttatatt
      781 cttgacctaa tgtctttccc cattgtgtga caaaagtttc taatttggaa gctttattcg
      841 tatcccacaa agtattctta acctcttcag aagaagattc agtgattgaa cttgcacttg
      901 tttccacaga ttgcttcatt tctgttgtag tttgttcagt tgattgcgtt ttactttttt
      961 tagtctcaat tgttgtatca gtttctatag tcgctttagt cctcgtattc tcagatgtac
     1021 aaccagtgaa taaaattccc attcctatta gtaataacag ccattttttc attatatttc
     1081 cccctttcaa tttctccctc acgtacttat tctatcagac aattaattga tctaaatggt
     1141 aatcttttaa tcgattcttt cccaataatg tcgatagccc gtatggcagc ggtctcttcc
     1201 ttgaggactc tcagatggat taaaactagt agaaatttcc cttccacatc ctctacagcg
     1261 ataagttctc attatttttg ggctattagt attatttttc ttagttagag ctatgagtcc
     1321 aactatgatt accccaactg tcttaattac ttttccttta tctatattcg gatgcgcaag
     1381 catatattta tataaccaat atccaccgta aattaaacca cctaatacaa tcagcttagc
     1441 catgtactcg tctcctttct ttttgtttaa ttactttttg aatccatctt gcagagaaac
     1501 cataaacttc tgtcaattga cggatatcta cagttttttc ctgttctatc atgttttcga
     1561 ttctttttct aactaactca cgatcgtaca ttttcattgg aaaagaaatc tgttgccctc
     1621 gaaaagcaag ataaaattga tagagcaatt ctgaatccag ttccatttcc tcaaaaaagt
     1681 cattcatact tttataagag ttactcaaac aagtttcatc aatctccatc tacacacccc
     1741 ctgttatttt ttacataatg aaaatagcat attactgggt acattgataa ttcatataac
     1801 atgtatcatc acatgtattt ttaaagtcaa aaagcatgta aaactactta taagtaacat
     1861 gagaaaataa aatgttattt ttcttgaaat aataattgac tatctgccaa ttgccattca
     1921 ttacgaacat gaatacttag tgggtgggat aacttacctt ctgcatttat caatactttg
     1981 gtcatcttct tttgtttcgc actcaattga tcgtaaatag tctttgcttg ttcataagcc
     2041 acttgactcg tcgctgtaat cgaaccacaa gcaaaaataa tggtattgat tttcttttct
     2101 gtaaaaacag tcgttaatat ttccaaagat tgttcatcat ttcctcgaag aaatacccga
     2161 ggacttttcg cttttgtgta cgaaaaaaga ttcacagcaa taaaaccagt aaatcctaat
     2221 tgtcggatat tcttttcgat taataacata ctcaaatcat tcgtaaatgg ttctgtatct
     2281 gttggacgaa tagtaatgac tactgctaat ttatcttccg cttcttttcc tttgatcgcc
     2341 cattgtcgtt tgaaaacata gcgatgggta tgatgaggat ctcgccaaac ttctgtttgt
     2401 gtggtcacta attctttttc aatcatctct ttttctccta tgctattttt tgttcaagca
     2461 cttcaattac cattgccgtt ataagtgtca atactagttg aaaatatttc atattctttc
     2521 tccttttttc ttcagaattt gatgaatcgc ggtaaggata tcttctaaac atttcgcaat
     2581 cattctcaaa taatcgatct tattcatccg ttcctccaat ctgactccaa accaatcgtc
     2641 cttctaccag taacccacga acaaattctc gctggctggt aatatctgta atgaaataaa
     2701 ctacatagtc ttttccatct tctggtgcgc aataaatata aagatgtgtc atcactaaac
     2761 gtggtagatg aacatcttca ggaagcatac ccatttcaat ttcctctttt tccatcgtaa
     2821 cggattcata gccccactct tcagaagcag tgaccagctg tgcacacttt tccagtactt
     2881 cgatagccgt ttctaacaag tcctttcgtt cctttctttt agatactagt attcttctgg
     2941 taataacatc gtttgatact cacccgaatc aatggcccat attttttgag acactgattt
     3001 caaacgaagc ttaggtgaaa tcagatactc ttttctttct tccggttctt cttgacgatg
     3061 aaccaacttt tgatttcctt tttccgtaga aaattcaaaa atttgaagat agtctaaagg
     3121 aaattgcttt tgaacgtttt gatcgattag attccaacat aaagcttgta attccaccgg
     3181 caaactgtcc attactccct ttgtgacata acgttatcct ttattttgaa acatttcttt
     3241 tccctccttt gccataaaaa tagcccaatc ttctattaaa gaagattggg cttcatggat
     3301 ataatatata tttgtaaata aagttaattc agttataatt atcaaaacta cacaattatt
     3361 aaagtttttc atatctcttt gtttagtaag tttttctaaa t
//
LOCUS       DAZKSU010000137         3397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000137 DAZKSU010000000
VERSION     DAZKSU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.145"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..275
                     /locus_tag="ACG8Z3_002744"
     CDS             <1..275
                     /locus_tag="ACG8Z3_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0824984.1"
                     /translation="LIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERINE
                     LIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            418..639
                     /locus_tag="ACG8Z3_002745"
     CDS             418..639
                     /locus_tag="ACG8Z3_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0824985.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            655..1497
                     /locus_tag="ACG8Z3_002746"
     CDS             655..1497
                     /locus_tag="ACG8Z3_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824986.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1560..3362
                     /locus_tag="ACG8Z3_002747"
     CDS             1560..3362
                     /locus_tag="ACG8Z3_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0824987.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1229 a          468 c          701 g          999 t
ORIGIN      
        1 aactcatcaa gctggctaat aagggtcaag attatgaaca tctagcagat gagattgatg
       61 agctgagaga caagcgacag ctccttttag tagaagatgc ctccctcagt ggcgagaacg
      121 agcgaatcaa tgagctgatt gaatttatcc gcaagaacaa attccgtacc ttagagtacg
      181 atgataagct tgtaaggaag ataatccaga gcgttacagt ctatgaagaa cacttcgtca
      241 tatccttcaa atctggcatc gaaatggaaa tatgaaaacc agaagtaaat agcctatgat
      301 tctacagtag agtcgtgggt tttcttttgc ttgctgatta taataatatt ttaataacta
      361 ttgttattat aaaccaaatg atttataata ttcttagcgt aaacttggag gtgctgtatg
      421 actacggcag aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt
      481 gctagacgta ttggacagac tccacagaat ttcaataaga aattacaacg agaaacggta
      541 accttagatg agttgaaagc gattgctgat gtgctaggtg tcaagtttgt gcaggctttt
      601 atattgcctg atggcaatga aataaagata ggtaattagg aggtgtaatt tgtattgaaa
      661 atcataacaa taaatgatgt tgagtatgct gtatttgctg ctaatgaagg tacttccaaa
      721 ccacaacctc atatcataga aacgaaatct ggtactattc cagaaggaaa acaactgagt
      781 ttattaaaag aatatcttaa gcagaatgat atttccccaa taaaaggagc aacaacatat
      841 tggtgcattg acaaggttct taagcttgat tcaagtaaag aaaaaactat aagtgaaaca
      901 atccataaac aaaaatactt atctttaact gaagaaaata ttgagaaaca gcataagttt
      961 gtaggtgcaa gctctaatta tgggaaagaa ggtctaataa tacatgatgt tttgaatgca
     1021 tttcctcttc ataatgattt aaatactatt gcgatgaaga ttgcggtaat tgatgtaaca
     1081 aattcaacac atctttctca atataagagc aggttgtctc tatatgattt ggctaaagta
     1141 attctggaga ttccaaattt tgatgatcgg ttggcaaaag gagatcctca gttgataaat
     1201 attatcgcaa ggaacattgg tgcagttaat atgttctcat ttgcatccaa gtactgtaca
     1261 tatcataatg tggaagtgtg tgggagagat gattactcca tctttgatgg tatcgttaaa
     1321 aatactcttc cacactatat ccaaggacta actacgaata agattgatac gtggcgaaga
     1381 agttttgatt acgaggcatt taacgaatgt gttggaaaac tacttgatga aaataatata
     1441 catattcctt ttagaagaag aaagttagat cacttcttgt ggtatgcgaa taggtagaat
     1501 ataagaaaaa ggcatcgtaa aagtgattct ggataaaatt aatgggggag gaaataatag
     1561 tgggattatt tgattttcta aagaaaaaac aaggaaaggt tagtaaacac gttgattatc
     1621 aactggcttc tagtctattt cgtactggta aagtttttgc tgaaaaattg gaagtggaca
     1681 tatcaaatgt agatgagttt cgtaaaactt ttatcgcttt tgatgtggaa acaactggcc
     1741 taaattcttc ttcagataga attgtagaga ttggtgcggt gctatttaaa gaaggagttc
     1801 caattcaatc attcggaact cttgtcaatc caaaagttag aattgcagcc tcagcttcgg
     1861 caataaataa tataactaat gaaatgctaa actctgctcc aaatgagcag gaagtttacc
     1921 cccagttggt agaattttta ggagatgcac tagatggaaa aatcactatg tgtgcacaca
     1981 atgcaagttt cgattttgga tttttaagta acacattttc tagacttggg tatgatgcta
     2041 aaattaagta tgttgatact ctgagtctct ctagaaaaca tataaaaggc attgtaaatc
     2101 ataagcaaga gactgtagcg gaatatttcg gattaataaa tgacactgcc catagagcgg
     2161 aatcagatgc agatatatgc ggaaagattt tttggggtgt tttagatatt attgaggagt
     2221 cgttggaaga acaaaaaaag caaatagaaa agatgactcc agacataaaa gaacttgaag
     2281 cttgtgcttt tattcaagat attattgaga aaaaaggatg cgacaccagt tggatcaggt
     2341 atcgaaaaaa cagtaataat tatgtcgatg taacttgtct atatacattc ttaaagttta
     2401 agtttgctaa gaagggaaat tacatcatta ttagaaaaga tgctacaaat ggaattgatt
     2461 tacctataga accttgtact gtaagtgaag gcggtacaac aaatgtgcga gtctattttc
     2521 gtagcccctt tgacttaacg cctctttcaa attatatttt cagtgcttat agcgattgtt
     2581 ataattctat gaatgaatat atgagtgtta gtaattacac taaaaaagaa gcagagcaaa
     2641 gccttagtat gctgaaatct atttcaaaat cagcgatgaa agagttatta ttcgatgcaa
     2701 aaaatcgtgg atatgatgag actgaagtta acctaaatat tgaaaaagtg atatctagag
     2761 aagatgtaat tattaacggg ttgcataatc gagtttcatt aaacaagata cgaaatatag
     2821 ggaattggga gaaaggattt gatgcaggtt atccatattg ggaacgtgga gaaactgcta
     2881 ggaaaaatgg caaagtagat gaagctattg cactttttga taaagctcga tataacggct
     2941 atgatgctcc tgcgctatat gattcttatg ctaaagctta tcgaaaaatt aaggattacg
     3001 ataatgaaat tctcattctt gatgaaggaa ttatgcgtaa aacacggcac gatgttggta
     3061 ctttagaggc tcgtagggat aaggcaatta agctattatt tgctaaacaa gaggcagagc
     3121 ggatagctaa ggaaaaatct gattttctaa aagcgaacaa aaaagaggat gcgattagtg
     3181 agcctagcaa gaagcgtgga agacagatta ttcaaatgac tgatgatgga acaataataa
     3241 aagaattcga tactatagct tctgctgtaa aagaagttgg cgttaactca aaaagtatta
     3301 gggatgctgc aaatggagtt caaaaacatg ctggaggata ctactggaaa tacaaagatt
     3361 agcagataat ctaagataac ctaggatctt cttaata
//
LOCUS       DAZKSU010000138         3220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000138 DAZKSU010000000
VERSION     DAZKSU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.146"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1438
                     /locus_tag="ACG8Z3_002748"
     CDS             <1..1438
                     /locus_tag="ACG8Z3_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFQ0824988.1"
                     /translation="SKKNQPKDIKDIVDTRMYKERDDLNYTLIINKDGNYSWRPLVLL
                     HPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILN
                     WWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLG
                     DKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRY
                     RDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEI
                     KASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKI
                     TSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALK
                     PNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEG
                     TIKDEEKYIKMSKKIYITEFDDFNDYPF"
     gene            1776..>3220
                     /locus_tag="ACG8Z3_002749"
     CDS             1776..>3220
                     /locus_tag="ACG8Z3_002749"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFQ0824989.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaaga
//
LOCUS       DAZKSU010000139         3070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000139 DAZKSU010000000
VERSION     DAZKSU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.147"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..1014
                     /locus_tag="ACG8Z3_002750"
     CDS             118..1014
                     /locus_tag="ACG8Z3_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0824990.1"
                     /translation="MIQYYYIKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1085..1300
                     /locus_tag="ACG8Z3_002751"
     CDS             1085..1300
                     /locus_tag="ACG8Z3_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0824991.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            1317..1589
                     /locus_tag="ACG8Z3_002752"
     CDS             1317..1589
                     /locus_tag="ACG8Z3_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0824992.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            1591..2454
                     /locus_tag="ACG8Z3_002753"
     CDS             1591..2454
                     /locus_tag="ACG8Z3_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0824993.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELAKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGL"
     gene            complement(2872..>3070)
                     /locus_tag="ACG8Z3_002754"
     CDS             complement(2872..>3070)
                     /locus_tag="ACG8Z3_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0824994.1"
                     /translation="IQNFKIMHYQHTLKFASKSYFHNFRVNHTQDQSLSDNTHDFNDK
                     IHVKPIDKKYTCNNRYLFVPI"
BASE COUNT         1162 a          469 c          549 g          890 t
ORIGIN      
        1 tctttttcaa aataaacata aaagcccttt aaagagggct tttatatatt aatcacaaat
       61 cacttatcac aaatcacaag tgattaatca caaatcactt gtgatttgtg attcctaatg
      121 atacaatatt actatataaa aaaagaatgg ggcgtagtta tggagaagga agaactaaaa
      181 atacttgaag aattaagacg tattttaaac agtaaaaatg aagcaattgt tatcttaaac
      241 aattatttta aaggtggtgt tggaaagtca aaattatcga ctatgtttgc ttatttgaca
      301 gacaaattta atttaaaagt tttaatgatc gataaggact tacaagcaac attgacaaaa
      361 gacttagcaa aaacatttaa ggtagaattg ccacgtgtca atttttatga aggcttgaaa
      421 aatggaaact tggcttcttc tattattcat ttgactgata atttagactt gatccctggc
      481 acgtttgatt tgatgttact gccaaaatta actcgctcat ggacgtttga aaacgaaagt
      541 agattgcttg ctactctttt agcaccttta aaaagtgact atgatctcat tattattgat
      601 actgtaccaa cgccaagcgt ttatacaaat aatgcaatcg tggcaagtga ttacgttatg
      661 atccctttac aagcagaaga agaaagtaca aacaacattc aaaactatat ttcctatttg
      721 attgatttac aagaacagtt taaccctgga ctagatatga tcggttttgt tccttattta
      781 gttgatacgg acagcgcaac gataaaatca aacctggaag aactgtacaa agaacataaa
      841 gaggataatt tggttttccg aaatattatc aagcgaagta ataaagtaag tacctggtct
      901 aaaaacggca ttacagaaca caaaggctat gacaaaaaag ttttatctat gtatgagaac
      961 gtattttttg aaatgcttga gcgaatcatt caattagaaa acgaaaaaga atagaatcac
     1021 aaatcacttg tgatttgtga ttgttgatga taaaataaga ataagaagaa atagaaagaa
     1081 gtgagtgatt gtgggaaatt taggcgcaca aaaagcaaaa cgaaatgata caccaatcag
     1141 tgcaaaaaaa gatataatgg gagataagac ggttcgtgtt cgtgctgact tgcaccatat
     1201 cataaaaatc gaaacagcaa agaatggcgg aaacgtaaaa gaagttatgg ataaagcctt
     1261 agaagaatat attcggaaat atttacctga caaactttaa aaggagtgaa aatgaaatgg
     1321 cagttacata tgaaaaaaca tttgaaattg aaatcattaa tgagttatcg gcaagtgttt
     1381 ataatcgagt attaaactat gttttgaacc atgaattaaa taaaaatgac tctcaattat
     1441 tggaagtcaa tttattaaac caattaaagc ttgcaaaacg tgtaaatctt tttgattatt
     1501 ctttagaaga attacaagcc gttcatgagt attggcggtc aatgaatcgt tactcaaaac
     1561 aagttttgaa taaagagaaa gtggcttaat atggcaaata tagtcaattt tactgacaaa
     1621 caatttgaga atcgcttaaa tgataattta gaagaattga ttcaaggaaa aaaagcggtt
     1681 gaatcgccaa ccgctttttt acttggtggg caaccagggt cagggaaaac cagtttgcga
     1741 tcagcaattc ttgaagaaac acaagggaat gttattgtca ttgataatga tacctttaaa
     1801 caacagcacc ctaattttga tgaactagcg aaactttatg agaaagacgt agtaaaacac
     1861 gtcacccctt attctaatcg catgacagaa gcgatcataa gccgtttgag cgatcaaggc
     1921 tataatttag tgattgaagg tacaggacga acaacagacg ttcctattca aaccgcaaca
     1981 atgcttcaag ccaaaggtta tgaaacaaaa atgtatgtta tggcagtacc taaaatcaac
     2041 tcatatttag gaacaattga acgatatgaa actatgtatg cagatgatcc aatgacagcc
     2101 agggcaacac caaaacaagc gcatgatatt gttgtcaaaa acttaccgac caatttagaa
     2161 acccttcata aaacgggctt atttagcgat ataaggcttt acaatagaga aggagtgaaa
     2221 ctctattcaa gattagaaac gccttctatt agtccgaaag agaccttaga aagagaatta
     2281 aatcgtaaag tatcagggaa agaaattcaa ccgactttgg agagaataga gcaaaaaatg
     2341 cttcaaaatc aacaccaaga aacccctgaa tttaaagcaa ttcaacaaaa aatggaaagc
     2401 ttgcagccac ctacaccacc aatacccaaa acacctaaac ttccaggact ttaaacctta
     2461 aaaaaggaaa agagtagtta ccaaaaaacg gtaactactc tttttttata aaaacatttc
     2521 ctcatgtttt aaaagctgat cgagaaataa agcacacaac ggtattcttt cttttttcag
     2581 ttggtacatt ttgcagtctg taacgtgtca tttactgttt gttaccttaa catatccagt
     2641 cgaaaacaca agggtatata aacgagcaag ctcgccctgg ttctgttgca aagttagaaa
     2701 aatatagcta attggggtcc cgagcgccta cgaggaattt gtatcgataa gaaatagatt
     2761 taaaaatttc gctgttattt tgtacattta acttgacggt gacatctctc tattgtgagt
     2821 tattagtggt acagttttca accgttttaa tcataaaaaa gtggtgcatt tttaaattgg
     2881 cacaaacagg taacggttat tgcaggtgta tttcttatct atgggtttaa catggatttt
     2941 atcattaaaa tcatgagtat tgtccgagag tgattggtct tgcgtatggt taaccctaaa
     3001 gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa
     3061 attttgtata
//
LOCUS       DAZKSU010000140         2941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000140 DAZKSU010000000
VERSION     DAZKSU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.148"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1500
                     /locus_tag="ACG8Z3_002755"
     CDS             <1..1500
                     /locus_tag="ACG8Z3_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0824995.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDD
                     TLLEVQNDKSVEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1508..2914
                     /gene="lpdA"
                     /locus_tag="ACG8Z3_002756"
     CDS             1508..2914
                     /gene="lpdA"
                     /locus_tag="ACG8Z3_002756"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0824996.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          980 a          556 c          707 g          698 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
      241 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
      301 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
      361 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
      421 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
      481 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
      541 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
      601 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
      661 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
      721 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
      781 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
      841 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
      901 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
      961 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
     1021 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
     1081 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
     1141 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
     1201 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
     1261 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
     1321 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
     1381 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
     1441 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
     1501 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
     1561 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
     1621 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
     1681 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
     1741 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
     1801 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
     1861 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
     1921 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
     1981 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
     2041 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
     2101 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
     2161 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
     2221 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
     2281 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
     2341 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
     2401 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
     2461 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
     2521 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
     2581 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
     2641 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
     2701 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
     2761 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
     2821 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
     2881 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
     2941 t
//
LOCUS       DAZKSU010000141         2628 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000141 DAZKSU010000000
VERSION     DAZKSU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.149"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            333..653
                     /locus_tag="ACG8Z3_002757"
     CDS             333..653
                     /locus_tag="ACG8Z3_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0824997.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            814..1719
                     /locus_tag="ACG8Z3_002758"
     CDS             814..1719
                     /locus_tag="ACG8Z3_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0824998.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1716..2489
                     /locus_tag="ACG8Z3_002759"
     CDS             1716..2489
                     /locus_tag="ACG8Z3_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0824999.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
BASE COUNT          817 a          464 c          500 g          847 t
ORIGIN      
        1 gtaagataaa gcttttttta atttattgcc tgctaatata taaaaataag gtctagttac
       61 tgttttttta aatgattaat aaataacaac tcctttctat gaaattgcaa agcattcatc
      121 ttcttttctg ctaacgatat atccgtcaat gcttgttaca ctttactaaa aagtatgaga
      181 ttgccgatat ctggataggt gtgctatact acagaaaaag atatacgatc tgccaccggg
      241 tagtaaaaaa gaggatgatc attccattag gtctttgaag ggatgattct cctctttttg
      301 tgtatttttt atattgaagg aggaacgtat gtatggcatg gatggaatta atcatagcag
      361 gaattttgga agtattctgg tcaacgatga tgaaatggag tgatggtttt tctaaaatca
      421 attattcttt ttacacagtg attggaatga ttgctagttt ttactttttg tcaaaggcaa
      481 tcaaaagttt gcctatgagt ctctcttatc ctatttggac agggattggc gcagttggtt
      541 cggtcattat tggtgtggtc ttgtttcatg ataagctgaa tctttccact tggttctttg
      601 tcgggttgct gctcatcagc atcattggca tcaaaatcac tagcggtcac tagatactta
      661 gacaagcaga caaggactga tcagaaaaag cggggctttt tatttgatat tcctctttct
      721 atgaaaaaac aagaaaataa gactaatgac ctagcgactt ctctactcat caggtgtatg
      781 cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt ccaagaatta
      841 tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt atctcctggc
      901 aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat gaaagctatt
      961 ttaggactga tccattactc agaaggaacg atccgtattg gtgaaaatga gatttctccc
     1021 tctagtcata aaggattaga acaagtagga gcattgatcg aatatccggg tatctatcca
     1081 ttcttaacag gttatgatca tctgaagtta ttctctgaaa caaacgatgt atctgctatc
     1141 gacactatcg tgaaacagct caagatggaa aaatacattc acaaaaaagc gaaatcctac
     1201 tcgctgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca tccgtctatc
     1261 gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga tgtgcgaaaa
     1321 atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca cttactcagc
     1381 gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaaaat catcaaacaa
     1441 tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc tacgacaaat
     1501 gatcaagctg ctcgaacgat cttgacagac gctggatatc ccttggaaga tcctgataaa
     1561 atcaagcttc taaaaaaaaa cgaagagtta ttaggaactg ttatccagtt attgtctgca
     1621 gaaaatgtcc atgtgacaga tgtccagcat ctgacgaatg atttggaatc ttccctattg
     1681 gaaattctgg attcagtaaa gggggaagaa gaaaaatgac gttgcttata aaacaagaat
     1741 tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta gttgctttgc
     1801 tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca gttgaaatga
     1861 ctgctcagct tttcagtgcc acatcttgga ttgtctttat catgattgct gctgcaagta
     1921 cgatcatttc aatggaatca caatatggta cattgaaaaa tctcctttat cgaaagtatt
     1981 ctcgaggaga aatattagta agtaaatgga tcacattagt tatttattct atttatttat
     2041 accttttagc tattattgtc accgtgctga tgaaactgat ccttttccct tcgatttcct
     2101 ttacggagaa gatttctact ggtcagacac tgatccaaag cttattcact tatactttgg
     2161 gtagttatat cggtttatgg ctaattttaa gtttagtgtt gatgattgct tgttttatca
     2221 atagttccgg tgcctccatc tcagcaggga tcgtctttta ttttgcttca tcaatcatat
     2281 ctggtatcct catcgcactc attcaaaaat gggagtggat caaatggaat ccaatcagta
     2341 tgctgaatct gcaaaaccaa attggcaacg aagagattat gaagcaattg actcatcttt
     2401 ctactaatca aatgctgttt ggcaatttag cttacattgc tttgttctta gcattgggat
     2461 acctcgtatt caagcgaaaa aatgtatgat ggttcattaa caaagaagtt gtgacagaag
     2521 tggccagttt caagaaattt cggcttattt ttagaagaag ctacttcttg aacaccattt
     2581 attcaaaaaa agaagcgatg acacgaatgt tgtcaccgct tctttttt
//
LOCUS       DAZKSU010000142         2560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000142 DAZKSU010000000
VERSION     DAZKSU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.151"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..903)
                     /locus_tag="ACG8Z3_002760"
     CDS             complement(<1..903)
                     /locus_tag="ACG8Z3_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0825000.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSES"
     regulatory      complement(927..971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1186..2154
                     /locus_tag="ACG8Z3_002761"
     CDS             1186..2154
                     /locus_tag="ACG8Z3_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0825001.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2267..2545
                     /locus_tag="ACG8Z3_002762"
     CDS             2267..2545
                     /locus_tag="ACG8Z3_002762"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0825002.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
BASE COUNT          838 a          466 c          524 g          732 t
ORIGIN      
        1 actttcggaa aaaagacgag tatctacttt tgtcataatt ttactgttag caggtttctg
       61 ccaaaattca taacgatcaa ccaatgtttg tccacgactt attccctgag tttccacagc
      121 ggtgaagctt ttgaaacctt cacagatggc cccctcgatg aaataagcaa cagctaacgg
      181 atcattgatg acacatccta aaattctttc ttgtttccag tggaagtcaa aataaaaacg
      241 agtgatcgct tttaaatact ctccctcttc aggattcatt tgacagcagt attcgaggat
      301 agtcggtgtc aagacgattt ctcttgtcac atctaaccca accatttcta tcgtttgttt
      361 gagattctcg aatacataag aagctgcttc aggatcacac cagtaattgt attcagcaac
      421 tggcgaacag ttaccatggc ttttgtaagt tccacccatc gagacaaaac gatccatatg
      481 tttcccaata tttgggttga ctttcaaagc aaaagcgata ttggtcaaag gacccaaagc
      541 aatgactgat gtatttgtct tttctttgaa ataatcagcc aaaaaatcaa cggcatggag
      601 aggttctgcc tgttttctca agatcatcgg gaaatttgtt tcccctaaac catccatacc
      661 atgagtgtct tgtgcactga tgaaaggttg cttcaaaggt ttttccattc cttgataaac
      721 aggaatgtct aaacgatcac agcgttccag gcatttcagc acattctgcg tacctatatc
      781 tgcagggaca ttcccacaaa ctacggtaat agcaactact tcgatatcag gggatttgac
      841 agctaatgat aaagctaaag tgtcgtcgat ccctggatcg caatcaataa ttacttttcg
      901 cattatttcc aatctccttt ctttagagat acttagtttt ttattctggg aagtttccga
      961 accagtccaa gacgagcgtc ttgattataa tgcacgaaat tagtatactg gcttttattg
     1021 cgaatgtaaa tgctctctta caagtaaatc tattgaggag aactttaagt aaatgataca
     1081 atgaaaaaga aatggaattt acgtgtgccg aaaataaaga atcctatgga tcggtacatt
     1141 ggataataga tagattatag tttggcagaa agcggcgggt gtacaatggt tattttttat
     1201 ttcattctta ttctgataat tatagggatc agttaccatt ttctttctta tttttatgca
     1261 aagaaattag taagagcggg agtacagaga ggatatgact ggtatgaaga cactggtcat
     1321 cgtttgatgg accctgctgc agtcacgtca gtagaaaaaa aacgacaaaa attagaagaa
     1381 gagtgtgaaa gcttttggga gcagggaaca cccaaaacaa tcaaaagtta tgacgggttg
     1441 aaactagctg gtcagatgtt catccatcca aataagcaga ataaatgggt catttgcgta
     1501 catgattacc gaagcacagg gaaaagagac atgtctcata taggtaaaag atatgcagag
     1561 aaaggattca acgttcttgt acctgattta cgtgcgcacg gagaaagtga aggagaaatc
     1621 atcggaatgg gatggctaga tcggctagat ttaattgcct ggatccaact catcttagat
     1681 gaacagccga atgcgtccat tattttacac ggcggatcaa tgggagcttc tacgatcatg
     1741 atggcaagtg gtgaaaaact tccatcagcg gtcaaaggat ttattttaga cagcggatat
     1801 gtttctgttt atgcagaatt ccgatatatg ttaagtaaaa tcactgtctt tcctaaaaaa
     1861 atggtcatga gatatgcgaa tcattatgcc cagaaatacg ccggttactc cttaaagcaa
     1921 gcatcagcta cgagacagct tggcagcaac catctgcctt tattagtcat ccatggtgaa
     1981 agagatcatt ttgtccctac agaagcagcg tacacgattc aaaatgctac ggcaggggat
     2041 aaagcactgc tgcttgttcc agaagcagaa cacttggaag catcgatgaa agatccgaaa
     2101 acctattgga cagtgatttt ttcatttatt gaacaaagaa taacgatgga ttaattttct
     2161 cttctttttt gtaagtaatt cgaggataaa taattgttta tagatctgga gactgctaaa
     2221 ataaggtagg aataaaaaaa tatcagttat ttgaggagtg cggcatatgg aaatcaaaga
     2281 agaaaaaaat cgtcttgctt tattcaatga tgagcaacaa gagattggag aaatgacatg
     2341 gtcagatgcg ggcccagaca tcatgatcat tgaccataca tttgtagatc cagcatatcg
     2401 tggtcaaaag ttagctgaaa agctagtgta tactggagta gaattagcaa gacgagacgg
     2461 gaaaaagatc attcctttat gcccttatgc aaaaaaagag tttgaaaaga aaccagaata
     2521 tcaagacgtc ttacgtcagt cataagaata acagctgaaa
//
LOCUS       DAZKSU010000143         2483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000143 DAZKSU010000000
VERSION     DAZKSU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.152"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACG8Z3_002763"
     CDS             complement(<1..300)
                     /locus_tag="ACG8Z3_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0825003.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACG8Z3_002764"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACG8Z3_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACG8Z3_002765"
     CDS             complement(1014..1304)
                     /locus_tag="ACG8Z3_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825004.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="ACG8Z3_002766"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="ACG8Z3_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DAZKSU010000144         2432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000144 DAZKSU010000000
VERSION     DAZKSU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.153"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..2285
                     /locus_tag="ACG8Z3_002767"
     CDS             132..2285
                     /locus_tag="ACG8Z3_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0825005.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          831 a          427 c          539 g          635 t
ORIGIN      
        1 gctgtgaaac aaaaagttga ttttatcttg aaattaggta aagaatttct tgctccgccc
       61 attttttctt ttttattaag aagtttttgg gtacaatgaa gcatgaatgt aaataggcgg
      121 aaggatagga aatgatgaaa gattttatga aaaaaatatt aaatacttct tggttagaaa
      181 aaataagaaa aggtgcacaa gaacctaaga acaaaacgtt tcgccggtcc catgttcctt
      241 ttcgcttaaa ttttcttttt ttcattatat ttacgttatt tgttgcgttg attgctagat
      301 tgggttattt gcagatcgtg aacggcgaag agatggaggc gcgagtgaag tctacttcta
      361 cgattacggt ccaagaaagt actcctcgcg gaatgatcta cgactccaca ggaaaagctt
      421 tagttacgaa taaagcgaat caggccatca catatacgag aggaacacaa gttacggcta
      481 aagatttgct tgaaatcgca acgaagctga actcgctgat cgatgttccg gtagatgaaa
      541 atctgacaga tagagataaa aaagattttt ggctggctga tcccaaacat ttgaaagaag
      601 ccacaaaaag actttcggca aaagaaaagc aattagggaa cagcgaacaa tatagtgcga
      661 tggtcaataa agtcacagct gatgagatta atttaaacga ggagcaatta aaaattgcta
      721 ccatctttaa acggatgaat agtgcatatg aattaaacac tgtctatatc aaaaactctg
      781 gtgttactga tcaagagtta gctgttgtgg cagaacgcgc ttctgaactt ccaggagttt
      841 cgactggcac agactggaca cgagaatata atgcagcaag ttcactgaaa agtattcttg
      901 gcagtgtgac gactgaaaag caaggacttc cagcagatga agcagagaaa tacctagcaa
      961 aaggttattc tcggaatgac cgtgtaggac aaagttattt ggaaaaacaa tatgaagatg
     1021 tcctgcaagg aaccaaaacc caatatgaag tttcccttga taatgaagga aatgtttcga
     1081 atcaaaaaga aatcttctct ggggaaaaag gttctaattt aatgttatct atgaatgcag
     1141 aattccaatc aaaagtagaa gaaattttga aaaggaatta tcaaacattg atcaacaacg
     1201 gcaaagcaca gtattcgcct ggtgcttatg cagtggctat gaatcctcag acaggagaag
     1261 tgctagcaat gactggtttc agtcatgaac aaggttccaa agaaatcacc gagaatgctt
     1321 taggcacgat tactagcgcc tttgatccag ggtctgtagt caaggctggt actttgacag
     1381 caggctgggc aagtaacgcg atcagcggaa atcaagtttt gattgatgag ccgattcgtc
     1441 tgcaaggtgc ttcagaaaaa agctctgtct ttaatcgaag cggacaagtt gctcttgatg
     1501 cagtcaaagc atcggaattg tcttctaata cgtatatgat caaagtggcg ttaaagatgt
     1561 taggactaga ctatacacct ggaatgggac tacctagttt agatgaagaa gctaaagctt
     1621 atcagcaatt gagagactct ttcaaagagt ttggtcttgg tacaacgact gggatcgatt
     1681 tgccgaatga atctcctggg atctcacgtt ctgttgatta tatgaagaag ttcaatgcgg
     1741 ataatggcaa agaatggtat gctccaggta actttaccga tttggcattt ggacagtttg
     1801 acacatatac accaatccaa ttagctcaat atgcttcaac agtcgctaat ggcgggaaac
     1861 gagtacagcc gcacctagtc aaagcgatct atggcaatga cgaaaatgga aacttgggtg
     1921 aagtgaagaa agaaattggc acaacagtag aaaatacggt caatatttct gcagaaaata
     1981 tggcgatcct ccgtgaagga ttccaccaag ttgtccatgg taccgatcct tacacaacag
     2041 ccaaaccatt agccagtgcg aaaatggacc tttccgcaaa aacagggact gcagaaacgg
     2101 ttgcggaagg tcatccagat attgcgacag tcaatagtaa tatcgtggca tacggaccaa
     2161 cagacaatcc ggaaattgcc atcagtgttg tcttgccgaa cttattagac gaacaagacc
     2221 acataaactt gacgattgct aaagaaataa tggacgcgta ttatgatatg ttcatggcaa
     2281 aataaaaaag aggtcgtgaa aaagccgctt agtttcgagt atgaagaaaa attttgtgaa
     2341 aatagcttct cacatttttg cttaaatttg acttcatacc gagaaactgg cttttgaaca
     2401 ccgtttactc ggcaaaaaag gttgtgacaa aa
//
LOCUS       DAZKSU010000145         2422 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000145 DAZKSU010000000
VERSION     DAZKSU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.154"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..290
                     /locus_tag="ACG8Z3_002768"
     CDS             <1..290
                     /locus_tag="ACG8Z3_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0825006.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(803..876)
                     /locus_tag="ACG8Z3_002769"
     tRNA            complement(803..876)
                     /locus_tag="ACG8Z3_002769"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Ile,seq:cat)
     gene            complement(1065..2084)
                     /locus_tag="ACG8Z3_002770"
     CDS             complement(1065..2084)
                     /locus_tag="ACG8Z3_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0825007.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2095..2301)
                     /locus_tag="ACG8Z3_002771"
     CDS             complement(2095..2301)
                     /locus_tag="ACG8Z3_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0825008.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          740 a          466 c          405 g          811 t
ORIGIN      
        1 atgagacaca attatggttt aaatttgacc cttcgaatag atttatcaaa gatttttata
       61 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
      121 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaaggatg
      181 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
      241 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa agtaggctac
      301 ctaaaaaggt agcccggaac gaattttatc accatacttg tgaaaggaga tattttttaa
      361 gttagtatta agattgtgta atatgatgat atctatattt tatagtatca gtgctataaa
      421 atcaaaaata agtcactaat taactaccac tccaattgta agcctttttt ctcacttttt
      481 ttcaaaaata tggtatgcct tttaatagct tcaaatataa aagagtttaa agcgtaacac
      541 acttatgggg aagtggcttg gggtgcgctt taaactcttc tttattatca tctcacaatt
      601 taacccaaat gtctttctat ttaaaaatca aagtaaaact tttcaaatat acagaaatat
      661 aactatgtga aacatccttt cattaatcca taaaaggata cataaaaaag ccactcattt
      721 gagtggcaat gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga
      781 acgattcaaa tggctacgtt aatgtaccct gtaggactcg aacctacgac cggacggtta
      841 tgagccgtct gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata
      901 aacaatcaac cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac
      961 tattatgggt gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata
     1021 gttgtcaata tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata
     1081 tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga
     1141 tcattcgttg cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa
     1201 gatacatgat agccaacatt tccagaagta ttataagctt gatttacatc tgatctataa
     1261 attccatcag ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata
     1321 tactcgtatt tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt
     1381 tctccaaact gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca
     1441 ccagctcgtg ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta
     1501 aatccatgag ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa
     1561 ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt
     1621 cccggttggt tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct
     1681 ctagccaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt
     1741 tgtaactcaa tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca
     1801 taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa
     1861 gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta
     1921 gccgtttcat gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg
     1981 cccaaattaa attcattgtt gatagtatag gcaaatccgt taattggcaa taaaaaaaga
     2041 gccgttaaca ggctcatcgc agtaatagta attttctttt tcatatctat cctcctattt
     2101 atctgatgaa attttcttat acgttcgatt tgatacacct aaaatactcc ctagaaatgc
     2161 tccaaaacct gtaataatca tcaccgttat atctgtgtac cgccagttaa caccttttcc
     2221 gacaagaccg acaaaagtcg ctaaagcggg gataactact atagcgatcc atttcaaaat
     2281 atcaaaggtt ctattgttca tactatccac ctcccttcat tggttgggct actttctttt
     2341 tcaatcgctt gttttcttct atcaatattt ctctttctga aagcaaggca tcaatttgcc
     2401 cgtccagttg ttcaatttta tc
//
LOCUS       DAZKSU010000146         2400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000146 DAZKSU010000000
VERSION     DAZKSU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.155"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            209..2071
                     /locus_tag="ACG8Z3_002772"
     CDS             209..2071
                     /locus_tag="ACG8Z3_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0825009.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2071..>2400
                     /locus_tag="ACG8Z3_002773"
     CDS             2071..>2400
                     /locus_tag="ACG8Z3_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0825010.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 taaaaaattt gaaaaagcgt ttgattattt atattacgac ttagtgcaat aaaaaacgct
       61 ttttaacagt acatgtagtc gtctttataa tagataaact ctgtaatatt agttaaaaag
      121 ctataagttg cacttagtta agtgcaaaca tttcatattg tcgttagttt agaaaacgct
      181 tatattagca atgtaaggag agatgataat gcagcagcta acaaaaagag aaaggcagct
      241 tttgatttac ttggtggaaa tgtctgaacc ggtaacaatc aaaaagttgg ctgaaaaaga
      301 aaatgttagt gttcggacga taaaatacga tctagatgat ataagagagt ggctgaatga
      361 gcggaagcaa gtcctatata gcaaacgtag tcagggaatt tggatgaata ttagtagctc
      421 tgatcgaatc aaattaaaaa gcgaattgat ggatgttaat cgttttgaaa tatttgcaga
      481 tcaaagtctc cgaatcaaac gtttactaat tatgctttta gtaacaaaag aagctattac
      541 tggaatgaaa atggccaacg cattagatgt cagcaaagat acgatcatga atgatttaga
      601 tgtgctggaa aataattttc ttaaggaagg attgaccttg aatcgtcagg cgcgaaaggg
      661 tttttggatc ataggagaag aacgtctagt tcgattaacg attgaagaga ttcttcaaaa
      721 agagttcacg gattatgaca tttataaatt aatgtcattg ttattgaatg gcggtgagac
      781 tgaatacttt gaaatgtatt ctgctactgc tacacctatc caagaggtat ttaatcaagt
      841 aatcattcga atgcgacact tactggaatt tgaaaatcta gagaaactga attacgcgga
      901 attactaaat attctgattc gagtgacaat tgctaccgtt cgcttacgca aagaggctac
      961 gattggtcgt tatcaattgg ttgctgaaca agaattagac aaaaacgatc tttcttatcg
     1021 cgtcatgcag caggtctttg attattatca gttgccactg ttcgaggatg aatatcatta
     1081 tatctatagt gatacattcg acacgattca ccagcaagac gtcatgaaac tagttgagaa
     1141 tgtgatttat aaagtaagta aagcaatcaa ccattctttt tatcaagatc cacaattgtt
     1201 gacgaatctg tatgctcatt tatccatgcg attatcgaga aagaaaaaat ttgtcaatga
     1261 atataatcct tttaaagaag atatcaagca gaaatacccg gaattatttg atgcgatcaa
     1321 gcaagcagtt cgacaagaaa taagtgaaca aaatattctg atcaatgatt ctttcgtggc
     1381 atatattgcc ttacactttt tggtttccta cgaaagagaa gaagatctac gaagcgttag
     1441 agccgtctac atttgttcaa cagggttggg tgtgactagt ctgatcaagc aaaagatcaa
     1501 tgaagaatta aataatatca ctatcgtggc atttgcttca gtattgaatg cgcaagaaat
     1561 gattgctacc aaagatcctg atttggtgat tagtattttt ccaattaatg gcatcgatcg
     1621 tcctttcatc aaagtccatc cattacccac tgaacaggac ttgaaaaaaa ttcaacaaat
     1681 aataaaaaaa atacttgctt cttccaatca gcaggaaagg cataacttga atcttagaga
     1741 aacgtccatc aataaagata ctttagaaga ctttagcaag gaattgattt tgaaagctta
     1801 tacgatttat gaacgtttac ttgtcatttt ggaagcagaa ttgatccaag aatatcgaga
     1861 agcttttttg cttcatgtat tcttaatggt tcatcgtatc acatttgatc aacaatatat
     1921 ggcagaaggt cgtcaagtag accagattat tttagacaaa ggaacactgg taggaaaaat
     1981 cgaagcagtt tttgcagaaa ataatttagg aatcaatcag tcagaaatca gtgcattatt
     2041 ctcatatata aaagaaggag cgtcaagtta gtgaaaatgt cagacgaggc tttaaaaatt
     2101 atcgaggaaa gtcaaaatcg ggaaaagtta aaacaagtga ttgatctaat tgaagagcga
     2161 ttagtagaac atgctattgt accaaccgag ttgcagtgga ccattctaat taatcacttg
     2221 aatgagatgc tgaaacgttc tcaaagaaag gaaacgattc ccgtagtcga tcgggatttg
     2281 ttcaaagaag tatccaaaga agccataact atttctgatg aaatagttag aagtatcggt
     2341 aatttaaccg atgatgaaat atatgttttg tcgattcatt ttgaaacagc aaaaaataat
//
LOCUS       DAZKSU010000147         2388 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000147 DAZKSU010000000
VERSION     DAZKSU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.156"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(493..1785)
                     /locus_tag="ACG8Z3_002774"
     CDS             complement(493..1785)
                     /locus_tag="ACG8Z3_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0825011.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="ACG8Z3_002775"
     CDS             complement(2059..2319)
                     /locus_tag="ACG8Z3_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825012.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          788 a          448 c          360 g          792 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaa
//
LOCUS       DAZKSU010000148         2364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000148 DAZKSU010000000
VERSION     DAZKSU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.157"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1451
                     /locus_tag="ACG8Z3_002776"
     CDS             150..1451
                     /locus_tag="ACG8Z3_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0825013.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1555..1755)
                     /locus_tag="ACG8Z3_002777"
     CDS             complement(1555..1755)
                     /locus_tag="ACG8Z3_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0825014.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            2151..2300
                     /locus_tag="ACG8Z3_002778"
     CDS             2151..2300
                     /locus_tag="ACG8Z3_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825015.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT          768 a          453 c          408 g          735 t
ORIGIN      
        1 cgtacaaaaa aaccgtcttt tcttggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctatttt acggcgaata cctacattgt cgatagaaac tgctttatcg
      481 ctaaacaagt gcgctataaa attttagaag agctaactga aaaacaagca atgacgacaa
      541 ttgctaagca ttgcggcgtt tcttggtcga ccgtttctcg gacactcgct tctctgatcc
      601 ccatgacaaa agtaaaaaga aattggttac cgcgttgttt attaatggat gaatttcgtt
      661 cactgaaaaa tcaagtcggt ccctactcct tttcttgtat ggatggcgat actggaaaat
      721 tactggatat tcttccttca cggaagaaaa aggctctcgt ttcttatttt atgcaatttg
      781 aacgacgcgc acgattgaag gtaaaggtca tcgtgacgga catgaatgtt tcttatgcct
      841 ctctgataaa ggaatgtttt ccaaaggcaa aactcgttgt ggatcgtttt cacattgtca
      901 aacatctcat tcgtaaattt gaggatattc gcgtacgaat catgaaaagc tttgatcgaa
      961 acgatccaac acaagccaaa tattatcgac aattaaaggc gttgtctcgg cttctgatca
     1021 aacgacagga caagcttgtt tatgacaaat ggaccaaatg gcgcaatttt ggttgggcgt
     1081 atctcaccga aagtgaagtg gtggatcgtc tgctttcgat ctctgacgaa ttacgaatcg
     1141 cctattttta ctatcaagaa attcttcaag cgtttcacga caaagaggcg gacactttct
     1201 ttaaacttgt aagaacgatg cccaattccg ttccaaaaga actgcattct ataaaaaaag
     1261 cttttataaa ctatgaatcc ggcattcgtt tagccctcga attgccttat tcaaatgcca
     1321 aaatagaaaa tctgcatacc cacatcaaag cattgaaacg agtcgcttat ggctttcgaa
     1381 gctttcggaa aatgaagacc agaattttct tactaaataa tctgattacc tatgaatcaa
     1441 aaaacatcta aagtagaaaa ggaattctac cttagatgtt cttatctcac tctatcagtc
     1501 ctatttgaca aagagccctt ttctttcgaa aagacggtta aaagtcaatt tttattaagc
     1561 tttaacaatg tttgttgctt gagggccacg ttgaccatct tcaacatcgt aagacactgc
     1621 ttggccttct tctaaagttt tgaagccgtc accttggata gctgaaaaat gtgcaaatac
     1681 atcatttcca tcttcaccag tgataaagcc aaaacctttg tctgcgttga accattttac
     1741 tgtaccgtta ttcataaata aattcctcct gatacgtatc aatgtacgtt ttttcgttgc
     1801 aataaatatt tgataggcaa aaggagtttt atctatgtag atatatcgct caaattacgt
     1861 ttcaaaatag attactcatt taatatatca tgctttttct aataacacaa ggataatgca
     1921 atgaaatata aaattcattg tcttctattg tcttcgaatc gaagaaaaag ttgaataacc
     1981 gcgattgatt cactttttgt tttcttgttt atttgctttt atctgcaact tttgacttta
     2041 aaatttctta actatgaaaa cctttcaatt ggtggtatac ttataaatgg aatttcgtta
     2101 tcaacgcgtt cgttgccttt cagcaagtat ttaaagaaag gagaatggct atgacattct
     2161 ataacaaaga tatacaatat cgagtaacct tggatacaga tttgaatcta tttgtcgttt
     2221 ttgacaaaga aaataccaat catgtagcga caggtgttac gatcgaacaa gcagtgcaag
     2281 aattaaaaaa atctgcataa ggagtggaaa ccctcatatg gacgattctc gttcttctct
     2341 ttaaacgcat ctactggtag ataa
//
LOCUS       DAZKSU010000149         2352 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000149 DAZKSU010000000
VERSION     DAZKSU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.158"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..544
                     /locus_tag="ACG8Z3_002779"
     CDS             <1..544
                     /locus_tag="ACG8Z3_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0825016.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="ACG8Z3_002780"
     CDS             complement(1017..1589)
                     /locus_tag="ACG8Z3_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0825017.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /gene="fic"
                     /locus_tag="ACG8Z3_002781"
     CDS             complement(1605..2210)
                     /gene="fic"
                     /locus_tag="ACG8Z3_002781"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0825018.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          740 a          469 c          359 g          784 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta gt
//
LOCUS       DAZKSU010000150         2334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000150 DAZKSU010000000
VERSION     DAZKSU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.159"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..2324
                     /gene="acm"
                     /locus_tag="ACG8Z3_002782"
     CDS             159..2324
                     /gene="acm"
                     /locus_tag="ACG8Z3_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0825019.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          915 a          351 c          454 g          614 t
ORIGIN      
        1 agttttttgg catatttatt ttttataaat atttgtaaaa taactatcaa ttttttagtt
       61 aaaagattgg aaatgagtgg gggaggaatt gaagaaagga tttttgagag atgatatagt
      121 aggttttatt tttagttaaa ataggggaga tagaaaaaat gaaaaagtgt agaaatatta
      181 ttttatcaat gttatttatt attactaata taacatcact gattcctgtt catgtctatg
      241 cggatgcagg cagagatatc agcagtaatg tcacttcgtt gaccgtggac ccaacgaata
      301 ttacagatgg aggaaacata aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc
      361 aaccaggtga ttatctttgg atcaattggc caagtgaagg aaatattcgc ggtgaaggat
      421 tccaaaaaga aatcccatta atgattgaaa ataaaaatgt tggaacttta acggtcagaa
      481 aagattctgc gcaggttgtg tttaatgaaa atattaaaaa cctagactca gtagaaggtt
      541 gggggcaatt tgagatacag gccagaaacg taaccgatac cggtgaagaa aatacgggac
      601 cattcaccgt gacgagcggt gataaaacag ctactgtaaa tgtgacaaag ccagcttctg
      661 gttcttcttc tagcgttttt tattataaaa caggagacat gctacctgaa gatactaagc
      721 acattcgatg gtttttgaat attaacaata acggcactta tgttgaacaa ccagttaaaa
      781 tcagtgatga aatccaaagt ggacaaagat tagatccaag tacgtttgaa attaatcaaa
      841 tacacttagg tgaacagaag gtttatagag gagaagaggg aatccaacaa tttttacaag
      901 atttccccag cgctacattt aatttttcag taactgataa ttacattgaa ataactatac
      961 caaaaaactt tgtcaatctt aggaaaataa tggtgagtta taagacgatc atagaaaacc
     1021 cagaacaaat aaattttgag aatcattcgg aagcttggtt taaagaattt aataaacctg
     1081 ctgttgatgg agaaagtttc aatcatacag ttaaaaatat ttcagcttct ggtggagtca
     1141 acggtacagt tagaggcgaa ttgaagattt tcaaatatat caatgacaca gagattggta
     1201 tcccaaatgt gactttcgaa ttaagacgag cagatgaaca gcctattcaa ggacagtcta
     1261 gtatcctgtt gacttcgaat gaacagggag aagctagtat caaaggcctg caagtaggag
     1321 actatgttgt aaaagaaaaa gaagcaccca attggattga ttttgatcca ctaagttcaa
     1381 atgaactcaa attctccata aacgaaaatg atacagaggg agtcagttta cctatttata
     1441 acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg gaacggtgga accactccaa
     1501 gaccgagtat ttattttaaa ttattcagag caagtacaaa caattgggaa ccagttcctg
     1561 atgcagaaac aaaacgatta gacaatggaa taacttcagt gacttgggaa gatatccaac
     1621 agtatgatga tagtggaaac gaatatacgt ttaaagtgca agaagtagat caaaacggaa
     1681 atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga
     1741 ataaagaagt catttcagtc tcaggacaaa aaacatggga agacaatgag aaccaagacg
     1801 gcaaacgacc gacgacgatc acagtaaatc tattagccga cggtcaaccg atccaacaca
     1861 aagaagtaag tgaaaaagat gattggcgct atcgtttcac aaatctgcca aaatacagag
     1921 acgggcaaga aatcatctat actgtaacgg aagacaacgt gcccgaatac agtacgacga
     1981 ttgacgggta taacatcaaa aacagttata caccggaaaa aacaaatata tctataccag
     2041 gtagccatat aacaccgtgg aaaaattttc cagagaagac agagagagag aaaattagaa
     2101 atctattctc tcaaaaattg caaatatttg ctcatacagg agaatccagc gaacaaagaa
     2161 gcaattatat tagtaagaga gctgtaccta aacagataac aaataaatca aaaagcattc
     2221 ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat cattggttta ttgatagtaa
     2281 ctatctgtgc tgggatattc ttacaaaaat ataaaaaagt ttagttagaa tata
//
LOCUS       DAZKSU010000151         2263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000151 DAZKSU010000000
VERSION     DAZKSU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.160"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8Z3_002783"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8Z3_002783"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0825020.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..1406
                     /locus_tag="ACG8Z3_002784"
     CDS             1131..1406
                     /locus_tag="ACG8Z3_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825021.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            1833..>2263
                     /locus_tag="ACG8Z3_002785"
     CDS             1833..>2263
                     /locus_tag="ACG8Z3_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0825022.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLD"
BASE COUNT          733 a          436 c          342 g          752 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttcca agaagtcata
     1381 aactctatgt gaatcagcaa ttttaacttc taactgttct ttattaatta aaaatgttct
     1441 gtaataaaat aaaactttat aacaactata acaaaaccga atgcaaaaca gaagttctat
     1501 aagttataat catagttgtt aggtgattca tctagtaaaa acaccagatt tcaaatttca
     1561 cacaatttaa actagatcaa tcatcaacct tcttcaaatt gttagaaccc tttcccgagg
     1621 agctgcagca taagcaaaaa aacaaacagg aagccaaatg gctaagacat tttttctatt
     1681 agtctagttg tttctcgtcg caagtttaag actttagaaa gttatttcta atgatgtttg
     1741 ggttgttttg atcaatgctt atgcacagct ccttacctat tatttatctc aacagatcta
     1801 acaaattaat ggactattta aggagttttt ttatgcttac gatctattct tctcgcacac
     1861 atagttacca tctgatggca aactggctga aagaccatca aatgccttac agagaacgaa
     1921 ttttcacacc taaacagcct ttaacaagga aagagatatt ttatttattg tcattaacag
     1981 aaaacggatt tgacgatcta ttagctaaac gctcgattga ttataaaaat ttgaaagacc
     2041 aaatcgacca atgtacgacc tcagagcttg tcgatctgat cgtctcgcat tttactttgt
     2101 tacaacgtcc catcattact gacggaaaga aattattagt cggattcaat gaagatagca
     2161 tgcgtctttt tatccctgta caatggcgca gaagtcaact gaaatctgtc tataacgatt
     2221 ttacatgggt aagcaatcta gaaaattcta cggatttaga tga
//
LOCUS       DAZKSU010000152         2162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000152 DAZKSU010000000
VERSION     DAZKSU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.161"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..391)
                     /locus_tag="ACG8Z3_002786"
                     /pseudo
     CDS             complement(1..391)
                     /locus_tag="ACG8Z3_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            461..622
                     /locus_tag="ACG8Z3_002787"
     CDS             461..622
                     /locus_tag="ACG8Z3_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0825023.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            641..1918
                     /locus_tag="ACG8Z3_002788"
     CDS             641..1918
                     /locus_tag="ACG8Z3_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0825024.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1928..>2162
                     /locus_tag="ACG8Z3_002789"
     CDS             1928..>2162
                     /locus_tag="ACG8Z3_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0825025.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHR"
BASE COUNT          812 a          233 c          379 g          738 t
ORIGIN      
        1 tcattttcga tcggtttcgc ttgataatag taggtctgac gagaaatatt tagaattttg
       61 cacatcgctg atatggaata tttatgcttg ttggcatcaa ttacttgtct tttcgtccga
      121 atatcagcgc cgcttgcttt aaaatatcat tctccatttc gagttgctta tttttctttc
      181 ttagtgcgat caattctgct tgttctggtg ttaagttgtc tctttctttg aatgagcccg
      241 ttgattgtgc ttgcttcatc catttatcga aagaagaagg cgtaagctca tactctcgaa
      301 taatttctgc gcgaggctta ccagcgagat agagatcgac gatttgttgt ttgaattctt
      361 ttgaataggt tcttcgttga cgtcttggca tgaaaaatcc tccagtgtgt tttttattat
      421 tctacacacc ttattttttc tgtctagtct agtgtagccg attcaggata aggataagtg
      481 tattaattgt ggaaaatgta aaaaagtttg ccaaatgaat aaagattcag ttgagaattg
      541 caatcatctg gagtgtatca gatgtggacg atgtaaaaat gcttgtccag tggatgcaat
      601 tagttatgga gtaagaaaat agagaggagt agttaggaaa gtgataaaaa atgctatagc
      661 atatattaca agaaagaaaa atagaacttt aataattttt atcatattaa ctatagttct
      721 ttcttgcttg tattcttgtt taacaataat gaaatcaagc aatgaaatag aaaaggcttt
      781 atatgaaagt tctaattctt caatatcaat aacaaaaaaa gatggtaaat attttaatgt
      841 taatcaattt aaagatattg aaaaattaaa agaaattgaa gaaaaaataa ttcaatatga
      901 tggattagca aaattaaaag atgctaaagt agttagcgga gaacaaagaa taaatagaga
      961 agatttatct gacgaattta agaatgttgt ttcactcgaa gctacaaata atactaaaag
     1021 aaatatttta tttagtagtg gagtatttac aattaaagaa ggtaaaaata tagaagaaaa
     1081 tgataagaat tcaattattg ttcatgaaga atttgctaaa caaaacaatc taaaattagg
     1141 tgatgaagtt gatcttgaat tactagatat tgaaaaaagt ggaaaaataa aaagtcataa
     1201 atttaaaatt atagggatct tttctggtaa aaaacaggaa acatatacag gattatcttc
     1261 tgattttagt gaaaatatgg tgtttgtaga ttattcaaca agccaagaga tattaaataa
     1321 ttcagaaaat aatgaaattg caaataaaat tttaatgtat tctggtagtg cagaatctac
     1381 agacttagcc ttaaacaaat tgaaagagct taaaattgat gagtcaaagt attttgttga
     1441 aaaagattct aatgcgtttg aagagtcttt agagtcagtg agtggaataa aatatataat
     1501 aaaagtaatg acttattcta ttatgttagg tgggatggtt gttctttcat taatcttgat
     1561 tctatggtta agggaaagaa tttatgaaat aggtatattc ttatctattg gaacatctaa
     1621 gatacaaatt ataatgcaat ttatattcga gttaatattc atatcgatac caagtataat
     1681 atcttcctta tttttaggaa atgtattact aaaagtaatt gtagatggat tcattaactc
     1741 agaggattca atgatttccg gtggaagttt aataaataat agtagtttta tgtcaaatat
     1801 aacaacactt ggacaaagtt atttaatatt aataagtatt attgttttat cagttgtatt
     1861 tgcttcttca ttaatgttaa tcaagaagcc taaagaaatc ttatcaaaaa taagttagga
     1921 gaaaataatg gatatattag aaataaagaa tgtagcatat agttatgcaa attctaaaga
     1981 aaaagttttg tcaggagtaa atcaaaaatt tgaacttggg aagttttatg cgatagtagg
     2041 aaagtcagga acaggaaaat ctacacttct ttccttactt gctggacttg ataaacctca
     2101 aacaggaaaa atattgttta agaatgaaga tatacaaaat aaaggatata gtaatcatag
     2161 aa
//
LOCUS       DAZKSU010000153         2121 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000153 DAZKSU010000000
VERSION     DAZKSU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.162"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACG8Z3_002790"
     CDS             complement(<1..426)
                     /locus_tag="ACG8Z3_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0825026.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACG8Z3_002791"
     CDS             complement(535..1566)
                     /locus_tag="ACG8Z3_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0825027.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          666 a          339 c          363 g          753 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 gatggattgg ataaattaaa ccatcacaac gagtggaaat tatatgaaat ttttcataaa
     2101 aattcatata gaaacaaatg a
//
LOCUS       DAZKSU010000154         2079 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000154 DAZKSU010000000
VERSION     DAZKSU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.163"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(458..1072)
                     /locus_tag="ACG8Z3_002792"
     CDS             complement(458..1072)
                     /locus_tag="ACG8Z3_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0825028.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1678..2055)
                     /locus_tag="ACG8Z3_002793"
     CDS             complement(1678..2055)
                     /locus_tag="ACG8Z3_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825029.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          688 a          405 c          318 g          668 t
ORIGIN      
        1 cattatctat ccttcaattt ttgtgtattt tatttctatt agaaaaaggt atcctgttgg
       61 acaattttgt tatatcaggt aaacttcatt gcaataatga taacaaatgt actataatta
      121 ctttgtaaag agttttgggg aagactcttt catcaacgtg tattaagaga caagaaagaa
      181 tccatatgga ttctttcttg tctctttttc cactactttt tatttttctg acactacata
      241 ttcttataac tcatttctca taactgtgaa acaacttata aaaataacaa actttaaaca
      301 aaacaagctt aaaatcaaag atgatccatc ttaaaataac taaaacaaaa aaagtagtcg
      361 ttgttaatcc tcattaataa tcgttttctt tttttcaaac gctccatttt tatgtggaac
      421 attatttttt taaggaactt tgtcaacatg tggataacta ttttccactt gtgtcctgga
      481 ctagctgttt tgctgtaaaa tacaaaactt gctgcagcaa aaagatcgtt gcaagagagg
      541 ctgttccgca gatcgctgga tctagatttg gcaaagtcaa gactgcatca tgtaaattgg
      601 cgtggttgtc caaaaccaaa tcagcaaatt caaataatcg caaaccactg ctatgttgcg
      661 atttgatact tcgtgacaaa tcaaatccag taacaatgat taatggatga ccattttctt
      721 tgatccactg agctagttcg atgattgccg ggtttcttcc ttcattcgaa agaagaagaa
      781 aaatgtcttc tgatttgaca gaaagctgat caataattgc ctgtcctgca ccttctagcg
      841 tttcatagac acctccggct ggattttcaa tcaatatact tggtacaaga tccggggttt
      901 gatcccaaaa ttcaaatgct acaccactta gtgtgcggct agcatatatc tgcaatctac
      961 caccttgatt aatttgtttc gcgatccaag tgccagcttg aagcatcagc ggctcttcta
     1021 tccttaattt ctcttccaat tggtgataat agtcaaaata acataaactc atttatctct
     1081 cccgcctagc tatacttgac atgaatagaa acagctgcct aatctaagga agtacataaa
     1141 aaaacagtag ttagacagta acacatgtgg tctttgctgt ttcaaacaca aaaaaggcat
     1201 tgaacgaata cgcccaatac cttgacatag caaataaaac aacgtgcaga ccatggaaaa
     1261 tatctacata ggggtagaat tttcacccta gattaggtac aataaattag gtcgatgcac
     1321 tgcattgact atggcaaaat gatcggtcag ggtgtatgtg tgtgactggt cattccctga
     1381 ttcttagcgc caactaaggg tcagggagcc tttttttatt cagctgtttc tatctgaatt
     1441 tattgtaagt aatctcttat ccaagtgcaa gcaagaaaat acaaaaaaac attggtatga
     1501 tttatatata tataaataac ataaaaaaag gaatggctta tcccgataca acaagcattc
     1561 ctttaaaaaa gattctttta tataagtgaa aaaagctttt ttccaccaat taaataaatc
     1621 tctcatctca atagtttatc cattatttta tctttttttg cgtataccta ctaaatatta
     1681 ccgtcttctt ctcgttcttc tactttgttc gagagaatct gtactagaaa aagtagttgt
     1741 atgtctacct tttctgccta tccgagactt tcttttcata tatgggatat aaatgattag
     1801 atacagtaca gcaaaaaata aagcaaatac gcaagcttcc caactaccta acgccaattc
     1861 aaccatcaag gtagcaaccc caacggatgc tgcataccag ataaaaatca gaaacgtttc
     1921 tttttttctg accatgcgaa acatgataaa tagactaata agcaatagtg gcaacacaaa
     1981 tatccaattg cttccttcca tcagccactt gagtatatca agcagcgact gtgcaacagc
     2041 gactaatttt cccatagtga acacacacct ttttttata
//
LOCUS       DAZKSU010000155         2018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000155 DAZKSU010000000
VERSION     DAZKSU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.165"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..500
                     /locus_tag="ACG8Z3_002794"
     CDS             108..500
                     /locus_tag="ACG8Z3_002794"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0825030.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            501..1940
                     /locus_tag="ACG8Z3_002795"
     CDS             501..1940
                     /locus_tag="ACG8Z3_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFQ0825031.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          845 a          150 c          344 g          679 t
ORIGIN      
        1 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
       61 aaacattgta tagcatttta cacaggagtc tggacttgac tgagtttatg gaagaagttt
      121 taattgatga taatatggtt tttgatattg ataatttaaa aggatttctt aatgatacca
      181 gttcatttgg gtttatagct aaagaaaata ataaaattat aggatttgca tattgctata
      241 cacttttaag acctgatgga aaaacaatgt tttatttaca ctcaatagga atgttaccta
      301 actatcaaga caaaggttat ggttcaaaat tattatcttt tattaaggaa tattctaaag
      361 agattggttg ttctgaaatg tttttaataa ctgataaagg taatcctaga gcttgccatg
      421 tatatgaaaa attaggtggt aaaaatgatt ataaagatga aatagtatat gtatatgatt
      481 atgaaaaagg tgataaataa atgaatatag ttgaaaatga aatatgtata agaactttaa
      541 tagatgatga ttttcctttg atgttaaaat ggttaactga tgaaagagta ttagaatttt
      601 atgatggtag agataaaaaa tatacattag aatcattaaa aaaacattat acagagcctt
      661 gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt ggatatggac
      721 aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa actgatgaga
      781 tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa ggaattggta
      841 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
      901 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      961 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
     1021 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
     1081 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
     1141 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
     1201 aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat ttagaaacta
     1261 atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct atactaggtt
     1321 ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg tcagaagaag
     1381 aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac ggtttagatt
     1441 atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa gagtatatat
     1501 tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt
     1561 ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg
     1621 attttagttg taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt
     1681 ttggagattc tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg
     1741 aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata
     1801 ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg
     1861 tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata
     1921 aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa gacattagta
     1981 ttaaatagtt taaaaaaatg aaaaataata aaggaagt
//
LOCUS       DAZKSU010000156         1948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000156 DAZKSU010000000
VERSION     DAZKSU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.167"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..806
                     /gene="vanR-A"
                     /locus_tag="ACG8Z3_002796"
     CDS             111..806
                     /gene="vanR-A"
                     /locus_tag="ACG8Z3_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0825032.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            784..1938
                     /locus_tag="ACG8Z3_002797"
     CDS             784..1938
                     /locus_tag="ACG8Z3_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0825033.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          641 a          371 c          424 g          512 t
ORIGIN      
        1 gaatcatcat cttaagaaat tcttagtcat ttattatgta aatgcttata aattcggccc
       61 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
      121 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      181 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      241 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      301 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      361 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      421 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      481 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      541 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      601 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      661 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      721 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      781 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      841 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      901 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      961 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
     1021 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1081 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1141 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1201 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1261 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1321 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1381 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1441 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1501 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1561 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1621 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1681 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1741 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1801 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1861 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1921 gataaaagga ggtcctaaga gatgtata
//
LOCUS       DAZKSU010000157         1857 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000157 DAZKSU010000000
VERSION     DAZKSU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.169"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..729
                     /locus_tag="ACG8Z3_002798"
     CDS             <1..729
                     /locus_tag="ACG8Z3_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0825034.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            787..>1857
                     /locus_tag="ACG8Z3_002799"
     CDS             787..>1857
                     /locus_tag="ACG8Z3_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NTF2-like N-terminal transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0825035.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFD"
BASE COUNT          672 a          316 c          411 g          458 t
ORIGIN      
        1 tctattttgt tactgcttac attagtagtt ggctattttt cgattgagtt tatccatgga
       61 ttttcgtctg caaaacagag ttcaactgtg aaaaaggtag agccgaaaag tatccctacc
      121 acactaaatg tggctttgat tggttcagat gctcgttcga aagacgaaaa cggtcgctcg
      181 gattcacttc tggttgcaca atacaaccag aaaacacaac aagcaaaact gatctctatc
      241 atgagagatt catatgtcga tattccaggt tacggaatga ataagataaa tgcagcttac
      301 tcttatgggg gagttgattt attaaatcaa acattaaagg aaaatttcaa atttgaagct
      361 ccctattatg caagtatcac atttcaagat tttatcgatt gcgttaatga actgtttcct
      421 ggtggagtca agatcgatgc agaaaaatct ctagatttag atggcgtata tataaacaaa
      481 ggagagcaag tgatggatgg taatacttta ctccagtatg ctcgatttcg ggaagacaaa
      541 gaaggagact tcgggaggat cagaaggcag cagcaagtga tcaaagctat ctctcagcag
      601 ctgaaagacg taacttcaat attcaagcta cccaaagcgg ttgggaaatt acttggtagt
      661 atccaaacga accttccaga aagtgtgctt ctggattgtg gtatggattt cctcaaagac
      721 atatgttaga ataaacaggt ataaatagtg aaataaagga atgacaagca agagaaggag
      781 gaaaaaatga aaagaagtga caagcacggc aaaaatcgaa caggcgctta tattgccggc
      841 gcagtgatct taatagcagc tgcgggtggc ggttattttt actaccagca ctatcaagaa
      901 acccaagcag tagaagctgg agaaaagaca gttgagcaat ttgtccaagc tttgaacaag
      961 ggagattata acaaagctgc agaaatgaca tcaaaaaagg cggcaaataa aagtgcatta
     1021 tctgaaaaag agatcttaga taaataccag aatatatacg gtgctgctga tgtcaaagga
     1081 cttcagatat cgaatctaaa agtagataaa aaagacgatt ctacttacag tttttcatat
     1141 aaagcaaaga tgaatacctc attaggtgaa ttgaaagatc tttcttataa aggaacatta
     1201 gacagaaatg atggtcaaac cacgatcaac tggcaaccca acttggtttt tccagaaatg
     1261 gaaggaaatg acaaagtaag cctgaccaca caagaagcag caagagggaa tattatagat
     1321 cgaaatggtg aaccattagc aacaacaggc aaactaaaac aattaggagt cgttccaagt
     1381 aaacttggag atggaggcga aaaaacagcc aatatcaaag cgattgcttc ctccttcgac
     1441 ttaacagaag atgctatcaa tcaggcgatt tcacaaagct gggtacaacc cgattacttt
     1501 gtcccattga aaatcattga tggagcaacg ccagaacttc cagctggagc taccattcaa
     1561 gaagtagacg gcagatatta tcctttgggt gaagcagctg ctcagctgat tggttacgtg
     1621 ggagatatca cagcagaaga tattgataag aatccagaat taagcagtaa tggaaaaatc
     1681 ggtcgctcag gtttggagat ggcttttgat aaggatcttc gtgggacaac aggtgggaaa
     1741 ttaagcatta cagatgcaga cggtgtagag aaaaaagttc tgatcgaaca tgaagttcaa
     1801 aatggcaaag atatcaaatt gacaatcgat gcgaaggcac aaaaaacagc ttttgac
//
LOCUS       DAZKSU010000158         1777 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000158 DAZKSU010000000
VERSION     DAZKSU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.170"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            242..1411
                     /locus_tag="ACG8Z3_002800"
     CDS             242..1411
                     /locus_tag="ACG8Z3_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0825036.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          637 a          281 c          287 g          572 t
ORIGIN      
        1 cgcttaattg gagccgttct tatggacctg catgatgaat ggatttattc ttcaagaaaa
       61 tacatcaatt ttgataagta gaaattgtaa aaacattgta tagcatttta cacaggagtc
      121 tggacttgac tcaaaaagat agcgtaaaat attttgtgta aatagaaatt tagggtagaa
      181 aaaaaagtcc attctgtaaa attaaagtta ccacacagaa attttatagg aggactttct
      241 catgactaaa tttactatag aaattatgca aacactactc aataaagggg atttagatga
      301 attatttcag gatcatttag agcgagcgat taattcactt ctacaagcag agctaactgc
      361 ttttttagat tacgaaaaat atgatcgaaa tggattcaat tcaggaaatt cccgtaacgg
      421 gaattattca cgtacattta aaacagaata tggcgagttg aatttaacta ttcctagaga
      481 tcggaatggc gaattttctc aacaaacact accagcatat aagcgaacca atgattcatt
      541 agaaacgacc attatccaat tatttcaaaa aggcattact atggctgaaa tctccaaatt
      601 aattgaaaaa atgtatggtc accactatac gccacaaaca atctccaaca tgagtaaatt
      661 ggtggctgaa gatgttttag cttttaaaga aagaacgtta gaagccaatt attccgttat
      721 atttatggat gcgacgcata tccctgtgaa gcgacagact gtttcgaagg aagcagtgta
      781 tattacgatt ggtattcgtt tggatggaac caaagaagtt cttgggttta ctattgcccc
      841 aactgagtct gcttatattt ggaaagaagt tcttcaagat cttagaaaac gtgggttaga
      901 agaagtttta ttagtagtga cagacggatt aagctgtatt gaagaaagta tccatagtgt
      961 gtatccgaat gcccaatttc aacaatgttg tgtgcatgta tctagaaata tcgctcataa
     1021 agttcgtgtt cgaggtcgaa aagaaatttg tgaggatttc aaattggttt accaagcgaa
     1081 ttcaaaagaa gaggcattgg atcacatcga ctttatgact aggaaatgga aaaagcagta
     1141 tccaagagtc gtcaatttac tcttgaatcc tgccctatta accttttata atttccctca
     1201 cgccatcaga cgaacaattt attcgacgaa cctgattgaa ggctttaata agcagctaaa
     1261 acgatatact cgaagaaaag aacaattccc taatgaagaa tctctagaga gattcttcat
     1321 ttctcaattt aatcaatata accaaaaatt tttaggtaga attcacaagg gatttaaaga
     1381 aattcaggat acattagagt cgatgattta actgtaatta acagaatgga ttttccattt
     1441 acacataatt cttgacgcta ccataatcta aataaccatc atattcatct actacttata
     1501 taattgagtt ttattaaact ttttaagttt ctattctatc aaatatttaa acaatctctg
     1561 catccgaaaa gactacctcg tatagtttta ctaacaatat ttttattagc gtgttcaaat
     1621 cctgatttct tgtatcaatt attttctgtt cactttcaat aagaaaaaag tctttacttc
     1681 catggataat aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag
     1741 aaaatatttt tattttaaag ataaaaaatt cttcatc
//
LOCUS       DAZKSU010000159         1723 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000159 DAZKSU010000000
VERSION     DAZKSU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.172"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..580)
                     /locus_tag="ACG8Z3_002801"
     CDS             complement(32..580)
                     /locus_tag="ACG8Z3_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825037.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(581..1438)
                     /locus_tag="ACG8Z3_002802"
     CDS             complement(581..1438)
                     /locus_tag="ACG8Z3_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0825038.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          485 a          347 c          247 g          644 t
ORIGIN      
        1 taaaggtagt tacttagttc ttctttgatt attatctaac tttattattc attccccacc
       61 ctttgcgcag tgagtcttcg gtatcttgtt ttatctgact ggtagttctt attatttctc
      121 cacgatcatc taattctgga acaattggat atttttctct gaacaagtct agttctttca
      181 ccacgtcatt aaacaaatct tgcttatctt cttgcgtaag attctgttta atatactcat
      241 ctaaagcttt tctaagcaat tcatctcttg tcatattagt ctcatacttt tctactaaaa
      301 ctctggaagt cgcatttaac aaagtcgaca gttttaaagg atgcttggtg gaataaacct
      361 tatcaaactt tctgattggt gcgccaactt tttttgttct tcctctactc tgtctttcaa
      421 cattggagga agtttttatt tcttgaactt ggtgcacttc taattgctta ttttcagatg
      481 gtgtaattgc ttgctgcttt ttctttgctt ctgcttcttt ttttattttg gcgagtcctt
      541 tattgttccc aaaaatatca agtcctttga tgtcagccaa ttactccacc tccgcatacc
      601 aattctcgtg ttcttccaat tctttcaaaa tactgataaa taattcgtgg cttttgcgat
      661 cccattgttt taagagacct ttctttgtct tctccatcgt aatacctgat ccgtcgtatc
      721 gttttaagcg cgcatttttg agtacaacat ttttaatcaa atgttcccca taaatctctt
      781 gggcttgttt atacatttcc tgatctactg cgtcaccgct ttccaacatg aaaggaataa
      841 caccagcaac atataaattt gtatcaaatt ctttcacaaa aaagtccata tactcaagat
      901 attgcatcgc atttctcatc gattttactt gggtctgtaa gacgacgatt gcccagtccg
      961 caatgtacat cgcggattta ctatatacag aaatagaagg tgggacatct aaatagatga
     1021 cgtcaaactc atcaaataat ggttctagct tttctttcat atatttgaat tgggataatg
     1081 aatcattata ttggtctttg atccaatcat catacatttc aaaggacatg tctgctggaa
     1141 gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga ttgtttatca
     1201 acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt
     1261 tagtgaagtt catatgcgtt aagttcatca catcagaagt atttccttgc atatccaagt
     1321 caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac atttgcgcgc
     1381 acgtactttt acctacgcca cctttaaaat taccgattac atatttcttc gctaccacgt
     1441 tcctacctct tttcttttag caatattcat ttgatactat tataacatat ttatttttag
     1501 ttactaccaa aaaaaagata tatatgtact accttataat taataaggaa aaggtagtac
     1561 ataaataagg aaaagctgaa atatattgat ttatagcttt cctcaaagta atgacgttat
     1621 ttaatttatc gagaagcaga gaatactcgc gactttagtc atgtagacga atcagatttt
     1681 cgataaaaaa gaagccgtcc ttcttaagaa cgacaacaaa tca
//
LOCUS       DAZKSU010000160         1720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000160 DAZKSU010000000
VERSION     DAZKSU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.173"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            387..575
                     /locus_tag="ACG8Z3_002803"
     CDS             387..575
                     /locus_tag="ACG8Z3_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0825039.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            653..1666
                     /locus_tag="ACG8Z3_002804"
     CDS             653..1666
                     /locus_tag="ACG8Z3_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0825040.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
BASE COUNT          576 a          278 c          361 g          505 t
ORIGIN      
        1 ttttagttag tctacattat tttggcgatt tgccaataag gactgttgag ttgtgcaaaa
       61 tcaaatctgg gtcagaatga acgtcattct gtctcagatt tttcgttatt tcaatttgat
      121 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
      181 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
      241 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
      301 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
      361 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
      421 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
      481 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
      541 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
      601 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
      661 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
      721 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
      781 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
      841 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
      901 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
      961 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
     1021 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
     1081 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
     1141 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
     1201 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
     1261 tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa cagcacagca
     1321 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
     1381 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
     1441 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
     1501 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
     1561 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
     1621 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
     1681 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc
//
LOCUS       DAZKSU010000161         1702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000161 DAZKSU010000000
VERSION     DAZKSU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.174"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..587
                     /locus_tag="ACG8Z3_002805"
     CDS             <1..587
                     /locus_tag="ACG8Z3_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825041.1"
                     /translation="VLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKPALGDIP
                     RHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRVPSQLWK
                     ELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIEKSNTQE
                     KKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(584..1180)
                     /locus_tag="ACG8Z3_002806"
     CDS             complement(584..1180)
                     /locus_tag="ACG8Z3_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0825042.1"
                     /translation="MQQNPSIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQE
                     VLDFVRDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLD
                     KFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLV
                     YRSIVQDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
     gene            1241..>1702
                     /locus_tag="ACG8Z3_002807"
     CDS             1241..>1702
                     /locus_tag="ACG8Z3_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HFQ0825043.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKT"
BASE COUNT          563 a          279 c          327 g          533 t
ORIGIN      
        1 aagtacttcc tacttttctt gctggaaagc ttggaattga tttatcagat agccagcatt
       61 atgatgaaaa cttagatgat ttaattacaa ctatatttgg tgttaagaga aaaccagcat
      121 taggtgatat acctaggcat attaaaggca gtcctttact acctatagaa cataatgaaa
      181 ataaagatat tgaaatctta ggaattatca ctgataaagt aactgtacct aaaatggatg
      241 gctcaagggg ttctgcatta tataaaattc catttagatt aaatagagta ccaagtcagc
      301 tttggaaaga gttgtttgtt agaaattgga attcaccacc acgatataca acaatgcata
      361 gaccaggaat tgcttctgtt gtagacgatg aaattttact taatggaaca actattgagg
      421 aagtccggga atatcatcgg gaaactttaa aactttgcat tgaaaaaagt aatactcaag
      481 aaaaaaaaat attgctagag gaggaacaaa aaaaagagag agagaatgag cgagtcagat
      541 cacataaaga aagtgttcag aaattagcag atgaattaga gttctagttg atagtcagtt
      601 ctttcttaat tcgatacaca gtttgtctag ttacattata gtctgttgct atttttgaaa
      661 tagcaacacc attctcctgg tcctgaacga tgcttctata aacaaggcga cgttgaggat
      721 cttttgtgtt cgcactgtat aacttaggac gtcctttata gacgccgttt gctttagcaa
      781 tctttatccc ttgtgcctgg cgtcgttttg attcagtacg ctcttgttca gcaatcatgg
      841 ccaagatttg aatgattagg tctttgataa atttatccag gagaggattt ccgattgctt
      901 cagccatgat cggtaaatta gtgataataa gtcgcacatt tttcttcttt agataattta
      961 ctgaatcgat aatctcgtca taattacgtc caagtcgatc gatagcctct acaataaaaa
     1021 tatcttggtc tctaacaaaa tctagaactt cctgaaaaat cggtcgatga gtaatagtcg
     1081 ctccagattg tttctcgatg aaaatttttt cagcaccaaa ttttctcatt tcctcgattt
     1141 gacgttgttc attttgatcg atcgatgggt tctgttgcaa agttttccaa aaaatctatt
     1201 ttagtgtaaa attgagaaaa aagacagaga ggacagagta atgaatcatt ttaaaggcaa
     1261 acaattcaaa aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag
     1321 ctatcgtgaa gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat
     1381 ttatcgttgg gtacaagagt acagcaaagt cctctattat ctttggaaga agaaaaatag
     1441 acaatccttc tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca
     1501 ttatctttat cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa
     1561 acgggatacg caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc
     1621 gaaagcaatt gtgaccgata aagcaccttc tcttggctcc gcctttagaa agttacagag
     1681 tgtgggttta tatactaaga ca
//
LOCUS       DAZKSU010000162         1663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000162 DAZKSU010000000
VERSION     DAZKSU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.175"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1613
                     /locus_tag="ACG8Z3_002808"
     CDS             147..1613
                     /locus_tag="ACG8Z3_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0825044.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          463 a          298 c          338 g          564 t
ORIGIN      
        1 atcttctttt ttttatgata ttgccattaa aaattacttg cttttataga aaattagttt
       61 atatttctta aataagtttt ttagttaaat ttttcggtaa agatgtataa tattttctga
      121 aaatttaaag aattggggta tagattatgg aaaaacagca agacaagggc ttcttcgggc
      181 agccgaaggg attgtcaact ttgttcttca ctgagatgtg ggaacgattt agttattacg
      241 gtatgcgtgc attacttatc tattatatgt acgatacagt tgccaacggg ggacttggat
      301 taccacgagc aacagcatta gctatcatgt cgatctacgg atctttgatc tacatgtcta
      361 gtatcgtcgg cggatgggta tctgaccgag tgttaggagc aaaacgcact gtattcttcg
      421 gtggagtttt cattatgctt ggccacatcg tcttggcttt accatttggt gtaagtacat
      481 tgtttgtttc gatggcattg attattatcg gaaccgcctt tttgaaacca aatgtttctg
      541 gcatggtcgg acatctttat tcaaagactg acttgcgaag agatgcagga ttttcgattt
      601 tttacatggg tgtcaactta ggggcattat tagcaccact aatcgttggt acaatcggtc
      661 aagaatataa ctatcattta gggttctctt tagctgctat tgggatgttc tttggattat
      721 tacaatacat catccaagga cgcaagacat tagacggagt gggtacaaca ccgccgaatc
      781 ctttgacaac tgaagaacgt aaaggattca tccgtaactt gatcatcgca cttgttgtta
      841 tcgctgcagt attcggcggg gcacaactaa ctggtcatct gacaattgac ttttttgtca
      901 atacaatcag tgtattgggg attatcttac cgttgtatta ctttatcaaa atgctgactt
      961 caaaagatgt aactgctgaa gaaaaaccaa gagtatgggc ttatatccca ttattccttg
     1021 cagcaatcat tttctggtca ttacaagaac aaggttcatc tatcttagct ttatttgcta
     1081 gtgaacgtac acaatctacg ctatttggtg taacgatcca agcaagctgg tatcaatcat
     1141 tgaatccaat tttcatcttt attctgacac cagtattcgt aacgatgtgg acgaaacttg
     1201 gtgaacgtca accatctaca gtagtgaaat tctctcttgg acttttattt gcaggattat
     1261 cgtacttgct attgatgttg ccgggtatgc tttatggtac aagtggaaaa gtaagtccat
     1321 tgtggttgtt gggaagtttc ttcatcgtgg ttattgctga attgtgtctt tcaccagttg
     1381 gtttgtctgt gacaacaaaa ttagcaccac gagcatttga atcacaaaca atcgctattt
     1441 ggttcttagc tgatgcttct tcacaagcaa tcaatgcaca aattgcacgc ttctacacac
     1501 cgggaacaga atctgcttac ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct
     1561 tgctatttac tgtgaaagaa ccaatcaaaa aactaatggg ctctattcat taatcaatga
     1621 acataaaaac aggaattgct tatttgcagc agttcctgtt ttt
//
LOCUS       DAZKSU010000163         1652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000163 DAZKSU010000000
VERSION     DAZKSU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.176"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..1602
                     /locus_tag="ACG8Z3_002809"
     CDS             55..1602
                     /locus_tag="ACG8Z3_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0825045.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          557 a          299 c          340 g          456 t
ORIGIN      
        1 gtttcttgtg gtattctgta acaaaatcag ttcgattcaa ggtggtgaaa aattttggaa
       61 actacagata cgttgcttca gttgcttcaa gaagcccata aaaccaatca agcacaacaa
      121 caaaccattc agaatcttac aactgaaatc caactattga atgaaaaggt caattattta
      181 accaacaaat tatttggacg ctctaaagag actctctttg aggaaacgaa tgggcaattg
      241 aatcttttta gtgatgaaga aatttctgta agtgttccag aagcagctgc caccattatt
      301 ccagtaaaag gacatcaacg agttgtagga acgaaagcag acaaaatcaa acacttacct
      361 ataacagaga aagaacatct ccttcctttg gaagaacaat tttgtgaaca ttgcggttca
      421 cagatgaaag atatcggacg gacaaaagta cgagaagaaa ttcgtttcca tcaggctatg
      481 ttggattgtc tcacccatta tcaacatacc tattgttgta aaagctgcga aaaagaagga
      541 ctttcttctt ttaaaaaagc gatcgttcct aaacctttga tttctaatag cttaggttcc
      601 aatagtttag tggcggaaac gatccggatg aaatttggtc agaaagttcc tgcctatcgg
      661 caagagaact attggaaaca aacacatggg ttggatattt cgagagataa tatcaccaat
      721 tggcatatta aagcagtgca aaacgcgtta gaccctttag gagaacgatt aagagtctat
      781 cttaatcaag aagagattct acacggggat gaaacaagtt atcgtgtgat tgaaagtgcc
      841 aaaacagata cctattactg gcagttttgt acaggaaaag acagtcagca tcccatcgtt
      901 tattatcacc acgacgaaag tcgtgctgga gatgttccga aaacttttct caaggagttt
      961 acagggtatt tacactgtga cggctatagc gggtataatg ccgtggaaag tgtacgactt
     1021 gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag caattccaaa aggaaagaaa
     1081 aacacggata ttcccgcagc tcaagcagtc aaacaattgg ataaatggtt tgtcctagaa
     1141 aagaaatgga aagacttttc tcctgagaag cgattaagct gtcggcaaca agaactgcgt
     1201 ccattattta ttgcttttta tgagtggatg gcaacgatag atcctgttgc aaaatccaag
     1261 ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg gttttgaacc tatttttgaa
     1321 gatggtcgtt tagaactcac aaataatcgt gcggagcgaa atatcaaaga gctggtgata
     1381 ggacggaaaa attggttgca ttccacaagt cttgaaggag cacgaacttc aggaatcatt
     1441 ctgagtgtat ataaaacagc agaacttaac ggattgaatc ctgtaaagta tctagagttt
     1501 ctatttgata agataccaaa tcttcccgta ctttcagcag aaactttaga tcaactgttg
     1561 ccttggaaca aggatgtaca acaacatttt tctcgtaact aaaaacgcca aacattcccg
     1621 attctattct ataggtcggg ttatttgacg ta
//
LOCUS       DAZKSU010000164         1482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000164 DAZKSU010000000
VERSION     DAZKSU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.177"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..921
                     /locus_tag="ACG8Z3_002810"
     CDS             367..921
                     /locus_tag="ACG8Z3_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0825046.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            977..>1482
                     /locus_tag="ACG8Z3_002811"
     CDS             977..>1482
                     /locus_tag="ACG8Z3_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825047.1"
                     /translation="MKITEFSEVFKTFSNIYNRIPEIEQNVVVDNDYYIENDFNKPRK
                     YDNLYNFIHNRTTINNNSNTRVLINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAI
                     NNGNLPSNKKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIF
                     ASIHDYIG"
BASE COUNT          592 a          156 c          256 g          478 t
ORIGIN      
        1 atgaaaagtt tggtagcacg gttattcata tgctaattta tttcacttta tagtaagggg
       61 agttcttagt atattgttca aacttaaatt tcatataatt taattatgta aattagctat
      121 tctaagaact tctacggata actgcccgtc taaaatttaa ataagagctt agaatcttca
      181 tataagtaag gttaattttt ttgtacaaaa aagtcggaat attctgagtt actgaaatga
      241 tataaaatca taaactaaac cccttgattt tcttcacttt taacagagta tatatataag
      301 ttcatgatta tggtaaaata tatatataat catgaactca gaatattttt aagaagggaa
      361 acaatagtga gctataatgt acaaccgtta aggacaaatg aagagataca agattttcag
      421 ttttggctaa gaagaacaag taatccagaa agagatagtt ttttattttt gtttggaatc
      481 aataatgggt tacgtatgtc agatattata ggattaaaag ttggagacat acgaggtaaa
      541 agtaagccta ttattgttga acataaaaca ggtaagcgaa aaccgatttt tatagataat
      601 ttgagagaag agattttatt atatactgaa ggtaaagaag aaaatgattg gttatttcca
      661 agtcggcaac aaggtcgaca tataacaagg gatcgtgtct atcagatata tgcagatata
      721 gcagaaaaat taggaagaga tgatatagga acacatacat taagaaaaac ttttgggtat
      781 cactactaca agaaaactag agacattgct accttaatgt ttatttttaa tcatagctcg
      841 caagcaatta caaaaaggta cattggtata actgaagatg aaataggggc atcgttacgt
      901 ggttttaaat tgggaggcta attgagatat taggatatat tttttgtgta taatgaaaac
      961 ttagaaggtg agattaatga agataactga attttcggaa gtatttaaaa cgtttagtaa
     1021 catttacaat agaataccag agatagaaca aaatgttgta gttgataatg attattatat
     1081 agagaatgat tttaataaac caagaaaata tgacaaccta tacaatttta tacacaatag
     1141 aacaacaata aataacaaca gtaacactcg agttttgatt aattcagcat attttcatat
     1201 taaatattta aagaaatatg agtatgaaga agttgtatta gattatgggc taaatagaca
     1261 gtcattttta atttatgcaa taaataacgg aaatttgcca agcaataaaa agaacataat
     1321 tattgaaaca tatgcctatc tggagggggg atctaaaaaa ctaattaagt catgtaatat
     1381 agagtatcaa ttatcagtag gagaaattaa atgtgttttc gaagaagatt tagaacttga
     1441 cttaaaaaaa atttttgcca gcattcatga ttatataggt tt
//
LOCUS       DAZKSU010000165         1456 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000165 DAZKSU010000000
VERSION     DAZKSU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.178"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1040
                     /locus_tag="ACG8Z3_002812"
     CDS             <1..1040
                     /locus_tag="ACG8Z3_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0825048.1"
                     /translation="EINQKYDETLKNVYSSLIVSNIEETSSSEIKNKLSLLKLKEKEY
                     IIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTREKIIKIFDKI
                     NNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRREID
                     REKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRENVL
                     QRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIF
                     SEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLAKAE
                     QYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="ACG8Z3_002813"
     CDS             1112..>1456
                     /locus_tag="ACG8Z3_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825049.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DAZKSU010000166         1438 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000166 DAZKSU010000000
VERSION     DAZKSU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.179"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..596
                     /locus_tag="ACG8Z3_002814"
     CDS             54..596
                     /locus_tag="ACG8Z3_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825050.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            752..1201
                     /locus_tag="ACG8Z3_002815"
     CDS             752..1201
                     /locus_tag="ACG8Z3_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0825051.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMAPIALDNEHPRI"
     gene            complement(1231..>1438)
                     /locus_tag="ACG8Z3_002816"
     CDS             complement(1231..>1438)
                     /locus_tag="ACG8Z3_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFQ0825052.1"
                     /translation="VKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQ
                     IRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          556 a          191 c          286 g          405 t
ORIGIN      
        1 tttggaaaaa taaaaaatta tatttcatca acgtttcata aaaggtgtgt agaatgttat
       61 tgaaagtagc tataaaatgc tattgtgtaa aaaataaagg aggaataatt tatgaaaaaa
      121 tagtaccaag aggagcatgc ctattacttg cattatgttt gagcttaagc gcagttgggt
      181 gtcaaaaaag taatgaaaac aataaaataa aagaaggaca atccataagt tctgaagagg
      241 aaggcatgtc aattaatgca aatggcaaaa atgaaacttt taagccatca gattatacat
      301 tggaggcaaa gaaagaatat gtttatgaat atttagggct taaatttaaa ctctcagaca
      361 aatttagaaa ttatatagct gataaaaaga tagcgatgtt agatgatcaa agtcctatag
      421 ataaagaact aaagtatgct attttgacat ttgagaaaat gacagaagaa caaaagaatg
      481 cagttataga aaagatgggc gatggatata aaaattggca aaatgagctt gaaagaattg
      541 ggactattgg tatatttgaa aaaaatacat cagaagaaaa aaatctaaaa ttataaaatg
      601 tgatactcat actaaaatag gagtttcaag tgatggaaaa tacgattgct atttaagcac
      661 aaatagcgga gctgaaagta atcttcttga tgaattaaaa agaactgaga ttcaaattat
      721 agataaaaaa gaacgtccta aaaatggttt tgtgctatca gaaaaaactg atttagaaaa
      781 tacagaagca tttaacaaag aatcggttaa agatttacgt aaactaccaa caaaggacat
      841 aaatggcaag gattttacaa gtaaagattt tgagaagtat gatcttacta tggtaaatgt
      901 atttgcaact tggtgtacag cttgtgtaaa ggaaattcct gatttggttg aggttcagaa
      961 tgaaatgaaa agcaagggtg taaatatcgt tggagttgtt acagatcctg ttgatgataa
     1021 cggtgaaaac aaggaagcaa ttgaaaagtc aaagttaata catgaaaaga caaaggcttc
     1081 atatcctttc ttaatgccag atggaatggc aacacttttt ttgacaaaaa ttataaggtg
     1141 ttgtgaatgc tgcggtgtta tggctccgat tgcccttgac aatgagcatc ctcggatctg
     1201 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
     1261 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
     1321 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
     1381 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgaca
//
LOCUS       DAZKSU010000167         1425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000167 DAZKSU010000000
VERSION     DAZKSU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.180"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..1392
                     /locus_tag="ACG8Z3_002817"
     CDS             100..1392
                     /locus_tag="ACG8Z3_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="HFQ0825053.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
BASE COUNT          463 a          292 c          258 g          412 t
ORIGIN      
        1 tgacaccagc atctttttta ttttccaaaa catatagaaa agacaccttg aaatccttta
       61 gaattaattt ggcgaaccaa cccaaaagga ggaatcaaga tgtcttacac tcaccttatc
      121 aaagaaacat tagatattct agacttatca gttactttta acgaaaattg tctcacaaaa
      181 gaaaaataca aagggcaaat ctgtcatatt tatcgtggaa acttgattta tacagcccaa
      241 gaatgcatcc actgtaaaca ccagattgct tcagatattg tgcgttgggg tacaacaacc
      301 gtccggttgt taatgaacga tgtttctgaa taccgtacct acctcgaatt gaaaaagcaa
      361 cgcttcaaat gcaaagcttg tcagcggacc tttgtcgcag atacttctgt tgcgaaaaaa
      421 cattgtttca tcagtgagaa agtccgctgg tctgtcgtga ctcgtctgaa aaaaaatacc
      481 tcaatgacag agatcgccgc tcagaaaaat ctatcggtct cttctgttta ccgtattatg
      541 aagcgatttt atcgtccgtt gaatcctttt cggacaccgt tacctaaggt actttgtttt
      601 gatgaattca aatcagttcg gggtgtttca ggtgctatga gtttcatcat gatggatgga
      661 caaacacagc gcttattgga cattgtcgag aaccgacaac tgcctttttt aaaacgttat
      721 tttagtcatt tttcacgaga aattcgtgaa gcggtcgaat ggatcgtgat tgatatgtac
      781 gctccttatg tatcgctcgt taaaaagctg tttccaaaag cacagctgat tattgaccgg
      841 tttcacatcg tccaacatat tggtcgtacc tttcgcaatc atcgaatcaa agaaacgaac
      901 caactattaa aaagcaaaga acaaaaacac tatcaattag gtaagcaatt aaaaagatac
      961 tggaaacttc ttcaaaaaga tgaacgcaag cttgattata ctcgacgcct ttggcgacct
     1021 ggcttcaaag ctcatttaac agagaccgat attgtggatc gcttactgaa agggagccct
     1081 gctttgcgtg taggctatca gttatatcaa gactttcttt acgctgtaaa ggaacgagac
     1141 tacgtatcgt ttgaagaact acttaccaat aatatcatgc ttcccgaagg ctatcagacg
     1201 accttgagaa cgttccagaa atttctgcca caaatcaaaa atgctttaca acaaagttat
     1261 tcaaatggcc cacttgaatg tctgaacaac catatcaaag tgttaaaacg aaatgcatat
     1321 ggctttcgta gcttttataa tttcaaattg cgcatcatga ttcgtcatgg caacgcattg
     1381 attttcaact aaaaaagatc aagaggaact gatccacttg atctc
//
LOCUS       DAZKSU010000168         1424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000168 DAZKSU010000000
VERSION     DAZKSU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.181"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG8Z3_002818"
     CDS             complement(150..1337)
                     /locus_tag="ACG8Z3_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0825054.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAZKSU010000169         1366 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000169 DAZKSU010000000
VERSION     DAZKSU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.182"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1366
                     /locus_tag="ACG8Z3_002819"
     CDS             82..>1366
                     /locus_tag="ACG8Z3_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0825055.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          466 a          263 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatca
//
LOCUS       DAZKSU010000170         1355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000170 DAZKSU010000000
VERSION     DAZKSU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.183"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG8Z3_002820"
     rRNA            <1..1355
                     /locus_tag="ACG8Z3_002820"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZKSU010000171         1289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000171 DAZKSU010000000
VERSION     DAZKSU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.184"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..992)
                     /locus_tag="ACG8Z3_002821"
     CDS             complement(36..992)
                     /locus_tag="ACG8Z3_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825056.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1117..>1289)
                     /locus_tag="ACG8Z3_002822"
     CDS             complement(1117..>1289)
                     /locus_tag="ACG8Z3_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0825057.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
BASE COUNT          411 a          211 c          198 g          469 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca acttgggagc gtcaataatt
     1081 ttgtgtaaat aaattgtcct cctgcaaaat aattagttac tcagtaaaca ttgaaactaa
     1141 tgtatcggtt acctgttgaa aacctttatg gcttctgttt agaaattttt gattgtatgt
     1201 atcaaaaatg ctgactagaa agcgttctag tgattcttca ttttgaaact gctcttttct
     1261 acggctgtat cttttaattt gcttattga
//
LOCUS       DAZKSU010000172         1289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000172 DAZKSU010000000
VERSION     DAZKSU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.185"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACG8Z3_002823"
     CDS             complement(15..1154)
                     /locus_tag="ACG8Z3_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0825058.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          336 a          281 c          239 g          433 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttatt
//
LOCUS       DAZKSU010000173         1273 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000173 DAZKSU010000000
VERSION     DAZKSU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.186"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..529
                     /locus_tag="ACG8Z3_002824"
     CDS             32..529
                     /locus_tag="ACG8Z3_002824"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0825059.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(566..1249)
                     /locus_tag="ACG8Z3_002825"
     CDS             complement(566..1249)
                     /locus_tag="ACG8Z3_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0825060.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          443 a          216 c          224 g          390 t
ORIGIN      
        1 tatagtatac tatgtcaaaa ggaggagtta gatgaacaat caaatcacac tagcgtattt
       61 agattatgat gatctagatg ctttacaagt tgtaagtgtt gtcagttttt atgaatcgtt
      121 tatcgatggc gcagatccac ttgatatgca aggatattta cagacacaat tgacaactga
      181 aatattggca gtagaattgg cacagtcaac cagtaaattt attggtatta aggatcatca
      241 aatactcatt ggttatatga aagtcaatga tgaaaaagat gctatagaga ttcaacggct
      301 ctatctgctc aaagattatc aaaacaaagg gttagggcaa cggttactcg atgaagcgaa
      361 tcgttatgct aaaacgaaac aaaaacgtta tttacgtttg actgtttatg aaaaaaatca
      421 tgcaggtatt cgtttttatg aacgtcatgg atttaaaaaa atcgggatca aacattttcc
      481 tttaggaaaa caagatcgta tttgtccgat tttagaaaaa gaaatctaaa aaaacgtcct
      541 cacttttgat gctgaggacg tttttttaga ctggtaatgg aaccataatt cgctttcctt
      601 tttgataaat cgctaattcc gtcacaccat ttgcttttaa cagagcaaaa agatcatcga
      661 aatggaaacg gaaatgttca aggtaatgcc catctgatcc aacactaaac aaggtaccac
      721 cacgtgaaag atataaatcg atgacatatt catataacgc taaattgtca tacaagtaag
      781 cgctcttagc atttaattcg aatgccaatt tatttttgat tacttgatct agaatcggta
      841 aaaactgcgc ttcatattgt ttaaattcag caacggatag accaaatacc cgaaaaccat
      901 agtcaaaatg agcaaagaca tctgcttcta taacaactag tgcttctgaa agttgtgcaa
      961 aatactgagg aatcacaata gcaggatcca tatctttgac ttcgtcatct aaataatcaa
     1021 attgcccatt atgatgcaca ctgagtaatt ttaaatcgta atctttatcg gctaaatagt
     1081 cgatgatccg atccttttca gaagcgatgt aaccgatttt gatgcctttt aaaacacgat
     1141 tgccaaaacg cttatttaag ctatcgattg tttgactgta catttcataa tcggggatat
     1201 catcgacttt agtaacagga ttggctaagt caaaatgttc agttgtcacg acgtaaccat
     1261 cgtagtttgt taa
//
LOCUS       DAZKSU010000174         1195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000174 DAZKSU010000000
VERSION     DAZKSU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.187"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG8Z3_002826"
     CDS             <1..1076
                     /locus_tag="ACG8Z3_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0825061.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZKSU010000175         1160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000175 DAZKSU010000000
VERSION     DAZKSU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.188"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(277..1158)
                     /gene="galU"
                     /locus_tag="ACG8Z3_002827"
     CDS             complement(277..1158)
                     /gene="galU"
                     /locus_tag="ACG8Z3_002827"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0825062.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          296 a          259 c          180 g          425 t
ORIGIN      
        1 aattaaaaaa ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct
       61 tatgtaatca ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt
      121 ctgaatctat tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag
      181 agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa
      241 atagcctttc attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt
      301 actcaactgg ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata
      361 ttcaatattt gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa
      421 ctctctcgca tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat
      481 ctcgtttcct gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag
      541 ataacgtccg ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa
      601 cccttttact ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt
      661 agatgtttca ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata
      721 atccatcatc aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat
      781 gacgacaaac ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc
      841 tcttggatga gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc
      901 gagtaattct gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa
      961 gtgatcttca atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga
     1021 atccaatgct tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc
     1081 ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac
     1141 tgcttttttg accttcactt
//
LOCUS       DAZKSU010000176         1153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000176 DAZKSU010000000
VERSION     DAZKSU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.189"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1153)
                     /locus_tag="ACG8Z3_002828"
     CDS             complement(<1..>1153)
                     /locus_tag="ACG8Z3_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0825063.1"
                     /translation="VFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLTRLVEDKKV
                     EKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLS
                     VLINGATGVGKSFLATKIFEYAIHEQIIEKDAPFAILNCADYADNPELLSATLFGYKK
                     GAFTGAEKDTDGLLATANNGYLFLDEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHS
                     AKVRFIFATTEKGENYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFFQNEADVLQ
                     KDIIVSREALSLMLSHPFSGNIGKLKNIIKISCADVYSRTQEEPLTIGKNEILIQLNM
                     LESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNE"
BASE COUNT          340 a          250 c          184 g          379 t
ORIGIN      
        1 ttttcattga aattaccttc atttatcagc tgagtaattt tttccaaaat cgaatcataa
       61 tcaaacggtt tgattcgatc cgtttttatc ttttgatttt tctttatcaa taaactagca
      121 atcggcaagc tttctacttc ggattccagc atatttaact gaatcaagat ctcatttttc
      181 ccaatcgtta atggttcttc ttgtgttcga ctatacacat ccgcacaact gattttaatg
      241 atattcttca gcttaccaat attcccacta aagggatgag ataacattaa agacaaggct
      301 tctcttgaaa caataatatc tttttgtaaa acatccgctt cattttgaaa aaataattga
      361 atcagctcta atcgttctcg aatggggcga tcttcaaaag ttggcaacat aacactaatt
      421 tgaatacgcc gatcaaaggt gtctaataaa taattttcac ctttttccgt cgtggcgaaa
      481 ataaaacgaa ccttagcaga atgccaattt acattttcac cgactggacg atagtttcca
      541 gtatcaataa atagaaagag cttttcttga ttttctttcg ataggcgatg gacctcatcg
      601 agaaaaagat agccgttgtt ggccgttgcc aataaaccat cggtatcctt ttcagccccc
      661 gtaaaggccc ccttcttgta tccaaacagt gttgccgaaa gcagttcggg attgtccgca
      721 taatcggcac agtttaagat agcaaatggt gcatcttttt caataatttg ctcatggata
      781 gcgtattcaa aaatttttgt ggctaaaaaa ctttttccaa cacccgtggc tccatttatt
      841 aatacactga gtccgttagg cggatatttg acagcggcta tacatttttg aatgacctct
      901 ctcagacttc cgtcatgacc aactacagaa tgaaaggctt cgtttatttg ctccgtatta
      961 tctacaaccg catattcatc taaaggcttc cacaaaacag gctttcccgg taatttctcg
     1021 acctttttat cttccactaa tctcgtcaaa taatggctgg cattctgacg agagatacct
     1081 agattcttcg aaattttctc ggcagtgaca gaagacatat tccctgttag tcgtatcagc
     1141 tcatcaaaaa ctc
//
LOCUS       DAZKSU010000177         1092 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000177 DAZKSU010000000
VERSION     DAZKSU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.190"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..821
                     /locus_tag="ACG8Z3_002829"
     CDS             <1..821
                     /locus_tag="ACG8Z3_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0825064.1"
                     /translation="RYKDIACKLKESICKQMTIDGTFGEQFLEGIGDEEKTTYSVKNY
                     QKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDPLYQNTMTFSAS
                     SFNPTYSEEIKGITWGLESGATFPGFITVLMSDMHNHQAFVTRLEELICLADLDGSWW
                     WWPYRLEAKQGEVVRDFGCGKCGWASGLFVSIMISQYFGLKFKKGVLQIDPVDNLTFS
                     WKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKKILHVEKGKTIYIQRRKR"
     gene            818..>1092
                     /locus_tag="ACG8Z3_002830"
     CDS             818..>1092
                     /locus_tag="ACG8Z3_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825065.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRI"
BASE COUNT          355 a          177 c          248 g          312 t
ORIGIN      
        1 agcgatacaa agacatcgcc tgtaaactaa aggaatcaat ttgtaaacaa atgaccatag
       61 atggtacttt tggggagcaa tttttggagg ggatcggtga tgaggagaaa acaacctatt
      121 cagtgaagaa ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
      181 ttgtggatgg gaaaatcaat ttattgatgc atgatggaga agaatcagac actacgttga
      241 tgccttttta tcaattttta gataataaag atcctttata tcagaacacc atgacctttt
      301 ctgctagttc tttcaatcca acttatagcg aggaaatcaa aggcatcact tggggattag
      361 aatccggcgc cacttttcct ggctttatca ctgttttaat gagtgacatg cacaaccatc
      421 aagccttcgt aacccgatta gaggagctga tttgtttggc agatttagac ggttcatggt
      481 ggtggtggcc gtatcgttta gaggctaaac aaggagaagt tgtgcgagac tttggctgcg
      541 gcaaatgcgg ctgggcatcg ggcctttttg tcagtataat gatcagtcaa tatttcggat
      601 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttcttgga
      661 aaaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc gagttgagta
      721 tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag
      781 aaaagggaaa aacgatttac attcaaagga gaaaaagatg agtatactaa aaaatattac
      841 ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa aatccaacag gcgagaaagg
      901 taaagctgcg atgttgccaa gcgagctagg accaagccgc aaaggtcgtg gcgcaattcc
      961 gctgacttct ggtgaagaaa ctgtgattgc ggatattact ggaacgggaa taattcgtca
     1021 tatgtggatg acgattcgtg atcgaacaga atttggcagt tttgttttac gtgatttaat
     1081 cttaagaatt ta
//
LOCUS       DAZKSU010000178         1091 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000178 DAZKSU010000000
VERSION     DAZKSU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.191"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ACG8Z3_002831"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ACG8Z3_002831"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0825066.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DAZKSU010000179         1049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000179 DAZKSU010000000
VERSION     DAZKSU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.192"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..469)
                     /locus_tag="ACG8Z3_002832"
     CDS             complement(<1..469)
                     /locus_tag="ACG8Z3_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0825067.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQ"
BASE COUNT          320 a          225 c          164 g          340 t
ORIGIN      
        1 attgcgtaat aaaatcatca ggcacatcga taatgtcgca agcaatatca gcacgtgaga
       61 aatgtggttc taaaaataaa tcatggataa agttcttcat cgaacccgcc aaaaattggc
      121 taattttatt tgggttaaag tcaattcgcc cttgcccttc ttgatatttc aaaatttcaa
      181 tgtaagcaat attctcatca tgcttatcca caatttgcca accatttaga ccaacagatt
      241 taacataacc attattttca ttcagtcgca ttaactgttc aaagtctaaa atcaatacat
      301 cattttgcgt gtgataataa atattctctt tgagttttcc aacaatggta attcggtcaa
      361 ttgaccactg tattttaagg ggggtggtca aagtttgcac acttaaccgc ctgttagata
      421 gggctgttaa attttcccac tcatttatga tcgtgccacg tttactcaaa ttttgttcct
      481 cctcaaagaa aaagagactt ttggaaagaa tttccagagt tcacgtaacc ctaaaaattc
      541 atatccaaaa atctctagct tgaagaaacc taaaaatatt cgtataatga acctgagtta
      601 cggagctagg tgaagtagtt tccgtaaata gtcgagagtg ttagcgcact gctcggcttt
      661 tattttgctc ttttttctcg ttttctttga ttatagcact cctcctaaaa aaataaaaag
      721 acctcaggga atttcattcc ccgaacccct tgagcctcct cctgccgaaa tggaaagggc
      781 gcacccgctg tttatttcat tcagctatcg gctttcacaa tctaacacga tcgttccgct
      841 accagtcgcc ttacaggctc cattttcgct cctctaacgt gttttaacga ttgtgccgat
      901 aactaaacga aataaacgct aaaaccgctc agaacgcaaa atatgccatt ttagagctat
      961 tgtagaaaat gtagtttgaa atgcaatttt cttctttcag ctttttattt cccacttgta
     1021 aatctgctaa ttattttcta ccagacaaa
//
LOCUS       DAZKSU010000180         1042 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000180 DAZKSU010000000
VERSION     DAZKSU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.193"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1042)
                     /locus_tag="ACG8Z3_002833"
     rRNA            complement(<1..>1042)
                     /locus_tag="ACG8Z3_002833"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          222 a          326 c          234 g          260 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat ca
//
LOCUS       DAZKSU010000181         1036 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000181 DAZKSU010000000
VERSION     DAZKSU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.194"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..502
                     /locus_tag="ACG8Z3_002834"
     CDS             <1..502
                     /locus_tag="ACG8Z3_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0825068.1"
                     /translation="QYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQE
                     TKRKIVPPEIKTWWRLELQLRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIA
                     GLISDQNLWAGLARHTKYKYRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLG
                     LDVTEE"
BASE COUNT          329 a          167 c          258 g          282 t
ORIGIN      
        1 gcaatatcgt gtggttgacc ctgtttcatt caaaccaatc tatgggcggt ctgggaagct
       61 ggaaacggca tattggggta gtcgtgccag tgagcgacaa ataaggatgt acaacaaaaa
      121 attggaacaa gaaaccaaac ggaaaattgt accaccggaa ataaaaactt ggtggcgact
      181 ggaattgcag ttacggcgtg gaaaagctac tgattggtat gcgatggttc atgaaagttt
      241 agacagtttc gcaagtcctc attttttgcc gactgataca aagcatactg atagaattat
      301 gattgcagga ttgatttcag atcaaaattt atgggctggc ttggctcgtc atacaaaata
      361 taaatatcga aatttgttga aaaaagaaag ccaaagtgat gagttagtta atcatttacg
      421 agagacattt tcggaatcgg cagatgtttt gaaaaaagaa ttggatacgt ggcttttggg
      481 gttggacgtg acggaagaat gagtggatga atttttgtcc acttttttat tttcagcaca
      541 agtcggcatg gtgggtcgtg cgtttgcagc aagagcgaaa aaacgctaac gaatccgcaa
      601 atgccgactt ggataaaaca gttgcgcaaa gagacttggc tttttataat aaatttaggg
      661 atatcccagt gtcactcttt ggggtaattc gggggaagga gttacccctc actctggaaa
      721 attagagcga agcagacgag attatcccaa tgaatatgac ggcttactcg gagtgatacc
      781 gagtaaaaaa gtcagataag tgagagtaaa tttacgtcaa tgtttaagat aagcgattat
      841 tttgcgtgaa gcaaaataaa gcgtaaaagg agttgagagc gtgacacgct ttcggctcct
      901 tttttcgttg tcacttgtga cagcgaaaac cctcggagag cgtttggaat tgtgacagcc
      961 atgtcacaat taggaaaatg ttactctttg tcaaagagta acagaaaggt ttacactctt
     1021 tttattagcg acagcg
//
LOCUS       DAZKSU010000182          943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000182 DAZKSU010000000
VERSION     DAZKSU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.195"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACG8Z3_002835"
     rRNA            <1..>943
                     /locus_tag="ACG8Z3_002835"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAZKSU010000183          912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000183 DAZKSU010000000
VERSION     DAZKSU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.196"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..912
                     /locus_tag="ACG8Z3_002836"
                     /pseudo
     CDS             1..912
                     /locus_tag="ACG8Z3_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          336 a          155 c          174 g          247 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      481 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      541 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      601 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      661 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      721 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      781 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      841 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      901 cttggctacg ag
//
LOCUS       DAZKSU010000184          911 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000184 DAZKSU010000000
VERSION     DAZKSU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.197"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..865
                     /locus_tag="ACG8Z3_002837"
     CDS             212..865
                     /locus_tag="ACG8Z3_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0825069.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          378 a           87 c          163 g          283 t
ORIGIN      
        1 gaagaaaaag tgttagtaga aaatcgcctt cgattttcca acaacacaaa atattataga
       61 agcctttttt ctaaaggatt tcaaggtgtc tgtttgcatt tattaaaaaa aagaaaaagt
      121 attggcaaca gaaggcgtca ccaacactag atattataga gccataatat gctgtataat
      181 atctagtgta taggcttaag tggaggtata tatgaaaata ttaactgttg aagatgataa
      241 tatgataaga gagggaataa gtgaatatct ttcagaattt ggatatactg ttattcaagc
      301 taaagatgga agagaagcct tgtcaaaatt taatagcgat ataaatttgg ttatcttaga
      361 cattcagata ccttttataa atggtttaga agtgttgaaa gaaattagaa agaaaagcaa
      421 tttaccaatt ctaatcttga ctgcatttag tgatgaagaa tataaaattg atgcatttac
      481 taatttagta gacggatatg tagaaaagcc attttcattg ccggtcttaa aagctagaat
      541 agattcttta attaagaaga attatggaca tttagagaag tttgaatata aggatctatc
      601 ggttaatttt aatagctata cagctaaaat aaatggtgaa gaaatagatg taaatgcaaa
      661 agaactagaa gtattaaaat gtttattgga taataatgga caagtgttaa caagaatgca
      721 aattattgat tatgtatgga aagatagcga agaaactcct tatgatcgag taatagatgt
      781 atatataaaa gaacttagaa aaaaattaca attagactgt ataactacta taagaaatgt
      841 aggatataaa ttggagagaa aatgaaaaaa ttaaagatat ttccaaaaat gttcatacaa
      901 atattttctg t
//
LOCUS       DAZKSU010000185          902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000185 DAZKSU010000000
VERSION     DAZKSU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.198"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..358
                     /locus_tag="ACG8Z3_002838"
     CDS             161..358
                     /locus_tag="ACG8Z3_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0825070.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            360..671
                     /locus_tag="ACG8Z3_002839"
     CDS             360..671
                     /locus_tag="ACG8Z3_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0825071.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            775..>902
                     /locus_tag="ACG8Z3_002840"
     CDS             775..>902
                     /locus_tag="ACG8Z3_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0825072.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          354 a           89 c          165 g          294 t
ORIGIN      
        1 gtaaaaacat tgtatagcat tttacacagg agtctggact tgactgatta aattcttatt
       61 caggaattat tttgctacgt ttcaagaagg aattgttttt tgaaatttaa tgaatgttag
      121 aataatagtc taaaaacatt aatttagggg tgattagatt atgaaacatt taaaaatttt
      181 gtctattaaa gagacacaac ttatctatgg gggtaccact catagtggaa aatattatgg
      241 aaatggagtg tattgcacta aaaataaatg tacggtcgat tgggccaagg caactacttg
      301 tattgcagga atgtctatag gtggtttttt aggtggagca attccaggga agtgctaaaa
      361 tgaaaaaaaa tgctaagcaa attgttcatg aattatataa tgatatatct ataagtaaag
      421 atcctaaata ttctgatatt cttgaggttt tacaaaaggt atatttaaaa ttagaaaaac
      481 aaaaatatga attagatccc agtcctttaa taaatagatt ggtgaattat ctatatttta
      541 ctgcttatac taataaaata agattcactg aatatcaaga ggaattaata agaaatttga
      601 gtgaaattgg aagaactgct ggaataaatg gtttatatcg agcagattat ggagataaat
      661 ctcaatttta atgatactta taatattaaa tatgtctcaa aattttttga ttagaaaaat
      721 aggtatattg aatcagtgac ataaaagtat ttatagtttg tagggggaat cgtaatggaa
      781 gaaaaaaata gactaaatgc taaacaatgt tcagatcaag agcttaaaaa aattaaagga
      841 ggtgctggaa caaaacctca aggaaaaccg gcatcaaatt tagtggaatg tgttttttca
      901 tt
//
LOCUS       DAZKSU010000186          876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000186 DAZKSU010000000
VERSION     DAZKSU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.199"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG8Z3_002841"
     CDS             complement(<1..434)
                     /locus_tag="ACG8Z3_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0825073.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8Z3_002842"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG8Z3_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0825074.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          143 c          137 g          333 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttg
//
LOCUS       DAZKSU010000187          874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000187 DAZKSU010000000
VERSION     DAZKSU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.200"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..604)
                     /locus_tag="ACG8Z3_002843"
     CDS             complement(<1..604)
                     /locus_tag="ACG8Z3_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825075.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDEL"
BASE COUNT          293 a          167 c          128 g          286 t
ORIGIN      
        1 tcaattcgtc atatcttcca aaatcaacat tgtactcata accaagaagt gtcggaatca
       61 ggtcaatttc aatttctttt tcaattgaac gtttaataag tttcgtggat acatacccga
      121 caaaaaagag aataataccc acttcaaaca aatacatgtg tccatttaca aagtgaataa
      181 acgtcaataa aaaatccttc actccttttg ttgttcttcc agattgttca atttgtttta
      241 ttaaagtcgc tagaattgca tttacaacaa ttaaatcgaa taggcttttg aaaagttttc
      301 ctgctgttaa tttttgcctg gtaaattgca tgactaattg agtaaaaata gacaatagca
      361 tgacaaaaat caacgtcatt agcactgtaa aaatgtctga tgctgcaatc ctattgggat
      421 catttatcaa gtaataagcc gttccaaata ctccaatcga gtaaacggtt aaatcaagaa
      481 taagtcgtgc cccgtaacta caaagttttc ctaatggcca taattgcaat acccaaaatt
      541 tctttcgttt gctatttctt ttcagaggaa gccaggtgtg ttcaatccag tcgtccgatt
      601 tcatttcgat ttcttcccta aattctttcc tttaacgttt tcctttgcta ctttagcagc
      661 gataggttgc tcaatgaacc aattttttaa gtacgccgat tgtaaaatta agctagacaa
      721 ctaaaaaagt catttgtgct acactcaaaa tagttccgtc caaagaatta taaggagtga
      781 gtacaaatga cctataccca tcttacaacg gatgaacttg taatgataga gtcttatttc
      841 aaaataaatc aatctgttgc gaaaactgcg catt
//
LOCUS       DAZKSU010000188          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000188 DAZKSU010000000
VERSION     DAZKSU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.201"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG8Z3_002844"
     CDS             <1..672
                     /locus_tag="ACG8Z3_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0825076.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKSU010000189          804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000189 DAZKSU010000000
VERSION     DAZKSU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.202"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>804
                     /locus_tag="ACG8Z3_002845"
     CDS             <1..>804
                     /locus_tag="ACG8Z3_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFQ0825077.1"
                     /translation="CQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIED
                     KMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRIL
                     PNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANIN
                     HYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFS
                     LLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKT"
BASE COUNT          265 a          164 c          155 g          220 t
ORIGIN      
        1 ttgtcaggta tctttgacca ccgttattcg tacgttgaaa gagtttaaaa gctacttacc
       61 aaagcaatct aagaggattc tccccagagt attgatggtt gatgaatttc gttcgcacgc
      121 ttccatagaa gataaaatga gctttatttg cgcagatggc gaaacaggac aattaattga
      181 tgtcttgcct acgcgtaaat tgcctcgatt aacaagctat ttcttaggct gcgctaatcc
      241 tgaagaagta aaatttttag tcacagacat gaacgccgcc tacttccagc ttaccaaacg
      301 tattctgcca aatgcgaaag tagtgattga tcggtttcat attatcaaac acatgaatca
      361 agcctttaat gagttgcgta tccgtgaaat gaatgaactt cgtaaagtag gacagaaaag
      421 tcaggcagaa aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa
      481 ccattatgaa tacaaaacat ggaaaagttt tcgagcacca aaatacccat ttcttacgga
      541 agcgatgatg attgatcgat tgcttggctt ttctacgtcc ctgaaggagg cgtatcccta
      601 ttttcatgaa ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt
      661 ggcagaactt cccgaaacgt tagatgacgg ctttcgggaa aagctccaaa accttctgac
      721 ctatgaagaa ggcatcacca acgcgatgat ctatccttac accaatggaa aaatagaagc
      781 gaagaatacc catataaaga caat
//
LOCUS       DAZKSU010000190          801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000190 DAZKSU010000000
VERSION     DAZKSU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.203"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..722
                     /locus_tag="ACG8Z3_002846"
     CDS             <1..722
                     /locus_tag="ACG8Z3_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0825078.1"
                     /translation="NEVLTINGLKWQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNI
                     YAAQETLKMGEKKFRTGLDKFIFGEDLDLPISMNPAQISNEDSFNSDILLADTGYGQG
                     ELLITPIQQAAMYSVFTNNGTLVYPKLIADKETKDKKNVISETAVQTIVPDLREVVQD
                     VNGTAHSLSALGIPLAAKTGTAEIKEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKE
                     DDDSATKRASELLQYLNQNYQ"
BASE COUNT          280 a          136 c          164 g          221 t
ORIGIN      
        1 caaatgaagt gttgacgatc aacgggctta aatggcaaaa agacagttct tggggatctt
       61 ttcaaacaac gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact gctttgatct
      121 attccgataa tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa aaatttcgta
      181 caggcttaga taaattcatt tttggtgaag accttgattt gccaattagt atgaatccag
      241 cacaaatttc taatgaagat agctttaact cagatatctt gctagctgat actggatatg
      301 gacagggcga acttctaatt actcctatcc agcaagcagc aatgtattct gtttttacca
      361 acaatggcac acttgtctat cctaaattga ttgcagataa agagacaaaa gataagaaga
      421 atgtcatcag cgaaacagca gtacaaacga tcgtgccaga tctgagagaa gttgtgcaag
      481 atgtaaatgg tacagcacat tctctttctg ctttagggat tccgttggca gcgaaaactg
      541 gtacagcgga aatcaaagaa aaacaggatg taaaggggca agagaacagt ttcttgtttg
      601 ctttcaatct tgataaccaa ggttatatga tggtcagcat gttggaaaat aaagaagatg
      661 atgattcagc aaccaaacga gcatcggaac tattgcaata cctcaaccaa aattatcaat
      721 aaaaaattgc tttttaagta cagtaaaagg actgtggcgt tatcgtcaca gtcctttttt
      781 caaataaacg gttttcacga t
//
LOCUS       DAZKSU010000191          791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000191 DAZKSU010000000
VERSION     DAZKSU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.204"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..716
                     /locus_tag="ACG8Z3_002847"
     CDS             <1..716
                     /locus_tag="ACG8Z3_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0825079.1"
                     /translation="KKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTST
                     ESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADK
                     SAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWS
                     ESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNE
                     TANNELKNGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DAZKSU010000192          790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000192 DAZKSU010000000
VERSION     DAZKSU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.205"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..782
                     /locus_tag="ACG8Z3_002848"
     CDS             <1..782
                     /locus_tag="ACG8Z3_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0825080.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          241 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc
//
LOCUS       DAZKSU010000193          780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000193 DAZKSU010000000
VERSION     DAZKSU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.208"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            9..>780
                     /locus_tag="ACG8Z3_002849"
     CDS             9..>780
                     /locus_tag="ACG8Z3_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0825081.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNE"
BASE COUNT          270 a          141 c          148 g          221 t
ORIGIN      
        1 gagtacaaat gacctatacc catcttacaa cggatgaact tgtaataata gagtcttatt
       61 tcaaaatgaa tcaatctgtt gctaaaactg cccattgctt gaatcgttca agacaaacga
      121 tccataaagt atatctgttc ttcaagcaag gaaaatcagc cttagaatat tatcaacagt
      181 ataagaaaaa caaatcaaac tgtggtagac gtccgcttgt tttacccgag gaacaatcag
      241 aatatattca aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag
      301 cgtttcctat tagttgttct gctcgtacca tttatcgtat gttcaaaaag gggctatttg
      361 attcttctga cttaccgatg aaaggcaagc gtaaaccgaa tggacatcaa gaaagacgtg
      421 gaaaacaaac tttccgccgc tctattcatg aacgtgaaaa ggattatagc caattctcaa
      481 atgagtttgg tcaccttgaa ggtgacacta tcgtaggtct gaaacataaa agtgctgtaa
      541 ttaccttagt tgaacgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag
      601 cgattgatat tgaaaaaaaa ttaaatcaat ggtttgaatc tgtaccgaaa aacctattca
      661 aatccatcac ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgtca
      721 atgatattgc catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga
//
LOCUS       DAZKSU010000194          764 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000194 DAZKSU010000000
VERSION     DAZKSU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.209"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(238..>764)
                     /locus_tag="ACG8Z3_002850"
     CDS             complement(238..>764)
                     /locus_tag="ACG8Z3_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825082.1"
                     /translation="LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDVEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          260 a          102 c           85 g          317 t
ORIGIN      
        1 taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat tagatatgaa
       61 aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag atgagagtca
      121 ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg atataggaag
      181 tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat ttttttgtta
      241 tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta tatcttctaa
      301 attagaagat atttgtatat caatatggaa tatattacct tttttgtatt tttttatgtt
      361 atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag ctggatttag
      421 tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt tttttatttt
      481 tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac ttggcagtat
      541 atgtatgtca atatttttca tctgtttcat aggtatttca acatctaaac tattaaaaac
      601 atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg gaacaataga
      661 tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat agcacatatt
      721 aagctcagtt tgtttatcat cctcaataat tttataaatc aaac
//
LOCUS       DAZKSU010000195          746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000195 DAZKSU010000000
VERSION     DAZKSU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.210"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>746
                     /locus_tag="ACG8Z3_002851"
     CDS             <1..>746
                     /locus_tag="ACG8Z3_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0825083.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKL
                     ALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLES
                     RFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          251 a          134 c          135 g          226 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaatc catgaaaagc
      481 ttgcccttaa gggcattact atatccgttc cgccacgtaa aaacatggat aaatcagaaa
      541 aactagacca cagcttactt ggaaaacaaa gaaaaacagt tgagactgtt ttttcttcct
      601 tagaaaaatt aggttgtcaa aatttcaact cacgttctgt taaagggcta gagagtagat
      661 ttgaaagcat attactggct tacagtgttc tattaagtcg agcacaacga cgttttgaag
      721 gaactttgag atattcttta ggatat
//
LOCUS       DAZKSU010000196          730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000196 DAZKSU010000000
VERSION     DAZKSU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.211"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..448
                     /locus_tag="ACG8Z3_002852"
     CDS             119..448
                     /locus_tag="ACG8Z3_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0825084.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            438..725
                     /locus_tag="ACG8Z3_002853"
     CDS             438..725
                     /locus_tag="ACG8Z3_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0825085.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKIIPVLLCGTHEKFYEQLKKLI"
BASE COUNT          283 a           80 c          138 g          229 t
ORIGIN      
        1 gttgaaattt attcagcaag gacgcattac gagtcacagt aattacttgt aaatacatta
       61 tctcgtggta taatgtattt actaagtaat tacatactgt tagaaatgag ggatttaaat
      121 gattacaaaa acaagaaaac aaggcaattc aatcatgttg acagtaccta aagattttaa
      181 tgttcctaat ggagtcgaag ttgaagcaaa gttggtagag aatggtattc tttatgaatt
      241 tgttgaacca caaaaagaat tttatgattt tagcgaagat attttgtctg atataattgc
      301 agaaggctac gataaagatg agattcttgt agaatttaag aatagaaaaa acaaaatgca
      361 ttcttccttt agagatattg ctgaagatac acttactaat tctaaagtaa tgactaaaga
      421 ggagttggca acagaaattg gcttatgaaa tcgttccttc aaaacatgtc attaagtatt
      481 taaaaaagct aaaagagaag ccattgaaag aaaaattttt gaatttaatt tatgatgaaa
      541 ttgcgattga tccatataag ggtgaacaaa aaactgggga tttatcaggt atttggtcta
      601 gtgggtttaa atatgcgggc acaacatata gagtagctta cgagattata gaaaacaaga
      661 ttattccagt attgttatgt ggaacgcatg aaaaatttta tgaacagttg aaaaaattaa
      721 tctagtgatt
//
LOCUS       DAZKSU010000197          729 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000197 DAZKSU010000000
VERSION     DAZKSU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.212"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..703
                     /locus_tag="ACG8Z3_002854"
     CDS             <1..703
                     /locus_tag="ACG8Z3_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0825086.1"
                     /translation="RRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQ
                     LIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNY
                     EKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRD
                     ISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNF
                     KLRIMIRHGKTFLTK"
BASE COUNT          235 a          147 c          136 g          211 t
ORIGIN      
        1 tcgacgcttg ccttatttag aacgctattt ttctcggttt tcattagcga ctcgtgaagc
       61 cgttcaattg attgttattg atatgtatgc gccttatgtt tcgttggtga aaaagctttt
      121 cccgaatgct cagttgatca tcgatcgttt ccacatcgtc caacatattg gcagaacttt
      181 cttgaatcat cgagtgaaag aaaccacggt acgacttaaa ggaaattccc actctcaacg
      241 agggttaggg aaaaaattga agcgctattg gaaactccta caaaaagaag aagcaaagtt
      301 aaactatgaa aaacgggttt ggcgtgccag ttttaaagat tatttgactg aaagtgagat
      361 tgccgatcga ctgttagctg cttgtcccaa cttacgccaa ggctatcagt tataccaaga
      421 cattctttat gcggtaaaga aaagagatca gtcactattt gaggattgtt taactagaga
      481 cattagcggt ctgccagaaa cctatacaac cactttacga acgtttaaaa agtttctgcc
      541 acagatccaa aatgcgcttc actacagcta ttcaaacgga ccattggaat gcttaaacaa
      601 ccacatcaaa gtattaaaac gcaatgcgta cggctttcgc aacttttaca atttcaaact
      661 acgaattatg attcgtcatg gcaaaacatt tttaaccaaa taaacagaac agaaagaggt
      721 tttcttctt
//
LOCUS       DAZKSU010000198          717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000198 DAZKSU010000000
VERSION     DAZKSU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.213"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..649)
                     /locus_tag="ACG8Z3_002855"
     CDS             complement(74..649)
                     /locus_tag="ACG8Z3_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFQ0825087.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          240 a          153 c          126 g          198 t
ORIGIN      
        1 cttaatttga caaataataa aatgaaaaaa cagagccaga ttagcattat tttgctaatc
       61 cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgg ggcaacatag
      121 tgatgactaa tgaaacaacg acgatagaca acaagcgatc aaggtagtct gtacccgcct
      181 gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc tgaacgatca
      241 tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc gaagaaacga
      301 tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg tttttatctt
      361 tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt tggattggcg
      421 aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg gacaatcctg
      481 tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg atagaatcga
      541 gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg atcccaatac
      601 acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt ttgttagccc
      661 atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DAZKSU010000199          700 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000199 DAZKSU010000000
VERSION     DAZKSU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.214"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            38..127
                     /locus_tag="ACG8Z3_002856"
     tRNA            38..127
                     /locus_tag="ACG8Z3_002856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            142..213
                     /locus_tag="ACG8Z3_002857"
     tRNA            142..213
                     /locus_tag="ACG8Z3_002857"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Glu,seq:ttc)
     gene            229..301
                     /locus_tag="ACG8Z3_002858"
     tRNA            229..301
                     /locus_tag="ACG8Z3_002858"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:262..264,aa:Val,seq:tac)
     gene            311..383
                     /locus_tag="ACG8Z3_002859"
     tRNA            311..383
                     /locus_tag="ACG8Z3_002859"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Asp,seq:gtc)
     gene            419..494
                     /locus_tag="ACG8Z3_002860"
     tRNA            419..494
                     /locus_tag="ACG8Z3_002860"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:452..454,aa:Phe,seq:gaa)
     gene            496..576
                     /locus_tag="ACG8Z3_002861"
     tRNA            496..576
                     /locus_tag="ACG8Z3_002861"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:530..532,aa:Tyr,seq:gta)
     gene            583..656
                     /locus_tag="ACG8Z3_002862"
     tRNA            583..656
                     /locus_tag="ACG8Z3_002862"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:615..617,aa:Trp,seq:cca)
BASE COUNT          157 a          161 c          194 g          188 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg ccaaactgtc tcaagggttc gaatccctta
      121 ctctccgtag caaatttacc tggcccgttg gtcaagcggt taagacaccg ccctttcacg
      181 gcggtaacac gggttcgaat cccgtacggg tcattttaat tgaatgttgg aggtttagct
      241 cagctgggag agcatctgcc ttacaagcag agggtcagcg gttcgatccc gttaacctcc
      301 ataatgaact ggttccgtgg tgtaggggtt aacatgcctg cctgtcacgc aggagatcgc
      361 gggttcgatt cccgtcggaa ccgtcgttct tgttattcaa ttaataattt taaaatatgg
      421 cgcggtagct cagttggtag agcaacggat tgaagctccg tgtgtcggca gttcgattct
      481 gtcccgcgcc accatggagg agtagcgaag tggctaaacg cgacggactg taaatccgtt
      541 ccttagggtt cagtggttcg aatccactct cctccatttt ataggggcat agtttaaagg
      601 tagaacagcg gtctccaaaa ccgttagtgt gggttcaatt cctgctgccc ctgccatggc
      661 gaatgtggcg aagtggttaa cgcaccggat tgtggctccg
//
LOCUS       DAZKSU010000200          677 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000200 DAZKSU010000000
VERSION     DAZKSU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.215"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            240..437
                     /locus_tag="ACG8Z3_002863"
     CDS             240..437
                     /locus_tag="ACG8Z3_002863"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0825088.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            421..651
                     /locus_tag="ACG8Z3_002864"
     CDS             421..651
                     /locus_tag="ACG8Z3_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825089.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
BASE COUNT          228 a           89 c          113 g          247 t
ORIGIN      
        1 tagctacatt attacgcccg aattaggtga taatgccgga ataatcggcg gattagcatt
       61 ggccagaaaa gcggtaagaa ataagcaacc ataattaatg aatgtttcat tgatactcat
      121 gtcaaaagta tttaaccgag taatcaaatg atcaaatgaa gttggataaa gcgaaaatga
      181 attcaaataa aaaggctcta agtcaaatcg tgttgttttc attaaatcac tgaaaggaaa
      241 tgcttctgcg tttccttttt tctttttgtt gtgcgctgta taccttgagg atattcactg
      301 gtggcgtaca cttaaatagt aatatattgt gttactttat gtctgtggta atagttatag
      361 ttgcttacca atgtatgttc atttttcgaa atgttgactt cataatcctt ggcttaatgt
      421 ttgaattttt ttggtgatag attttttcag ccgtataaaa taaatcgtaa ttctattata
      481 gagagttgtg attataaaaa gatagcaaga aaaaaaaaga aagtttctat acattttaaa
      541 tattttattc ctatttctat tgctaaaaaa gtagaaattc tatatttacc tttaactctc
      601 gcttctcttg caaatgtagt tagcatttta atcgataata aactcaggta gttatattac
      661 cttgaaatga ttgataa
//
LOCUS       DAZKSU010000201          661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000201 DAZKSU010000000
VERSION     DAZKSU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.216"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>661
                     /locus_tag="ACG8Z3_002865"
     CDS             <1..>661
                     /locus_tag="ACG8Z3_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0825090.1"
                     /translation="RYKNIACKLKESICKQMTIDGPFGEQFLEGIGDEEKTTYSVKHY
                     QKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMPFYQFLDNKDHLYQNTMAFSAS
                     SFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNPQAFVTRLEELICLADLDGSWW
                     WWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQYFGLKFKKGVLQIDPVDNLTFS
                     W"
BASE COUNT          198 a          112 c          150 g          201 t
ORIGIN      
        1 agcgatacaa aaacattgcc tgtaaactaa aggaatcaat ttgtaaacaa atgactatcg
       61 atggtccttt tggtgagcaa tttttggagg gaatcggtga tgaggagaaa acaacctatt
      121 cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt aatcttttta agtgatgtga
      181 ttgtggatgg aaaaattaat ttattgatgc atgatggaga agaatcagac actacgttga
      241 tgccttttta tcaattttta gataataaag atcatttata tcagaacacc atggcctttt
      301 ctgctagttc tttcaatcca acctatagcg aggaaatcaa aggcatcact tggggattag
      361 aatccggcgc cacttttcct ggctttatca ctgttttaat gagcgagctg cacaaccctc
      421 aagctttcgt aacccgatta gaagaactaa tttgtttggc agatttagac ggttcgtggt
      481 ggtggtggcc gtatcgctta gaggctcaac aaggagaagt cgtgcgagac tttggctgcg
      541 gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat gatcagtcaa tatttcggat
      601 tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg ttttcttgga
      661 a
//
LOCUS       DAZKSU010000202          652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000202 DAZKSU010000000
VERSION     DAZKSU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.217"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..>652
                     /locus_tag="ACG8Z3_002866"
     CDS             96..>652
                     /locus_tag="ACG8Z3_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0825091.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMM"
BASE COUNT          202 a          127 c          121 g          202 t
ORIGIN      
        1 cttcaccaac atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga
       61 attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag
      121 ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa
      181 aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt
      241 gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc
      301 ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat
      361 ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact
      421 gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca
      481 tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc
      541 gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg
      601 aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg ga
//
LOCUS       DAZKSU010000203          632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000203 DAZKSU010000000
VERSION     DAZKSU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.218"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..239
                     /locus_tag="ACG8Z3_002867"
     CDS             <1..239
                     /locus_tag="ACG8Z3_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HFQ0825092.1"
                     /translation="GNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGK
                     ETLVKGIKNKEKKPYNAVVKIGEKGYIDFISFSK"
     gene            367..606
                     /locus_tag="ACG8Z3_002868"
     CDS             367..606
                     /locus_tag="ACG8Z3_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0825093.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          270 a           74 c          118 g          170 t
ORIGIN      
        1 gtggcaacaa tattgtatta aaaaaatcgt tttatggttg ttcaaattat cctgaatgta
       61 agtttacttt agctgaacat tttagaaaga aaaaactaac caaaacgaat gtaaaagaat
      121 tactggaggg aaaagaaacc ctggtaaaag gaatcaaaaa caaagagaaa aagccctaca
      181 atgccgttgt aaaaattggg gaaaagggat atattgattt tatatctttc tcaaaataaa
      241 cataaaagtc ctttaaacag ggcttttata tattaatcac ttatcacaaa tcacaaatca
      301 cttatcacaa atcacaagtg atttgtgatt gttgatgata aaataagaag gaatagaaag
      361 aagtgagtga ttgtgggaaa tttaggcgca caaaaagaaa aacgaaatga tacaccaatc
      421 agtgcaaaaa aagatataat gggagataag acggttcgtg ttcgtgctga cttgcaccat
      481 atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa aagaagttat ggaaataaga
      541 cttagaagca aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg
      601 aattgaagtt aaattagatg ctaaaaattt gt
//
LOCUS       DAZKSU010000204          619 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000204 DAZKSU010000000
VERSION     DAZKSU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.219"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..379)
                     /gene="tnpB"
                     /locus_tag="ACG8Z3_002869"
     CDS             complement(26..379)
                     /gene="tnpB"
                     /locus_tag="ACG8Z3_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0825094.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(369..563)
                     /locus_tag="ACG8Z3_002870"
     CDS             complement(369..563)
                     /locus_tag="ACG8Z3_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825095.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          179 a          133 c          103 g          204 t
ORIGIN      
        1 acattttttg tgttaacccc ccagcttaaa aaactccttt ttttcctggc aaaatagctc
       61 gaggttgatc gatacttaat ccttctaaca accatcgaac ctgttggttc gttaaggttc
      121 gaacttcgtc tttcgtccga ggccatttta acttgccgtt ttctatccgc ttataacaga
      181 gaagaaaacc atcaccgtcc caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa
      241 ataaaaaaac ggcatcttcg taaagctcga gttgatactc ttgttgaata agactggcaa
      301 gaccatcaat tccttttcgc aaatctgttt ttccgcacac aatataaata tgtttggttt
      361 gaagtaaatc ataaagcatt ggacaacagt tctttcaaaa tagcttgaag aatataattg
      421 ttaataccgt tatagatcaa aacttcagaa ccattctctc ttttgatgcg catcgctaac
      481 ttggcagccg cgggtttacc tttttctggt gcactctttt cgtttcctaa ttggacagga
      541 acaatctcat ttaatttata catagaaaac ctccagactt cttgatagct caagtatatc
      601 ttgaggtgca tttgttagt
//
LOCUS       DAZKSU010000205          617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000205 DAZKSU010000000
VERSION     DAZKSU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.220"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>617)
                     /locus_tag="ACG8Z3_002871"
     CDS             complement(<1..>617)
                     /locus_tag="ACG8Z3_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0825096.1"
                     /translation="EMPFQTSAKITIENLHPKDMSSFFYTINYALVDELEENSLYFHA
                     NWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLERYWWGEGEFKFYIDGDSEYPT
                     VTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYPYQSNRDATRDRFETGKLNAV
                     HAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGNDDVRLFER"
BASE COUNT          177 a          140 c          122 g          178 t
ORIGIN      
        1 tgccgctcaa ataagcgaac atcatcgttg ccgatttgtt gtaaagtgac cgataaatct
       61 tcggtaaaac agatggggtc ttgaagatgc caacgataaa gaccatgtcg tggcattgcg
      121 tcgtcaccaa aggcatgaac ggcattcaat tttcccgttt caaaacgatc tcttgtggca
      181 tctctatttg attggtaagg ataccctaaa aaggctgttg aatagttttt cgcatgtgga
      241 cgtccgaact catcgtactc atgaaaggcc cacgccccgc caaaataatc ttctgaacct
      301 gttgaggtta cggtcggata ctcactatcc ccatcaatgt agaatttaaa ctcgccttcg
      361 ccccaccaat aacgttctag gcaggtaagc gcaaggtaag tacccacgta atagccttga
      421 ccctttaatc gatcaattaa cttataatcc tcctgtaatt tggtaatgcg ttcccgatgc
      481 caatttgcgt gaaaatatag gctattttct tccaattcgt ccaccaaagc gtaatttatc
      541 gtataaaaaa atgaggacat atccttcgga tgtaaattct cgatcgtaat tttagcggat
      601 gtttggaaag gcatttc
//
LOCUS       DAZKSU010000206          613 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000206 DAZKSU010000000
VERSION     DAZKSU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.221"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..484)
                     /locus_tag="ACG8Z3_002872"
     CDS             complement(53..484)
                     /locus_tag="ACG8Z3_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFQ0825097.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          214 a           80 c           68 g          251 t
ORIGIN      
        1 tcttaattgt acaattagta ttatctgcac tactttctaa ttaaataaat ttttagttat
       61 taacttgatt aaaatcagag ataatatcat ttaattcagt ttgaagggtt gtaacgatta
      121 aatggaaatt ttcttcatct gttttattat atggggatgt tttcgttata tataacaact
      181 tttctttttc aattttcatt aataactctg tcgaagtctg tactttatct tgataattaa
      241 atgcagacat accactttgt ataattgtaa ttattgctga agcaattgaa acaatcaata
      301 gtaaagacct gtgttcggaa gcctgatcaa ttaatattgg gatactagct gataagacta
      361 tttttataat atttccgata ccaattactc tattataaaa tcgaatttct ttttttaatc
      421 tatcgattgt agaatcaata gattcgataa acgctttttg atccatcatt ttctctccaa
      481 tcattttatt aaaatcatta acaaaaatat tataatagga tttaagtaag ttctaactaa
      541 tcacatataa aaataaaaaa acatttgtat tttatcaaag ttttattata cttgtcaatt
      601 tgtgtacatc cta
//
LOCUS       DAZKSU010000207          607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000207 DAZKSU010000000
VERSION     DAZKSU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.222"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>607
                     /locus_tag="ACG8Z3_002873"
     CDS             <1..>607
                     /locus_tag="ACG8Z3_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0825098.1"
                     /translation="DLEFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYN
                     IGKYKERDIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYE
                     ILANQKKITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYI
                     ENSYGNNKISNMDKIFDVKFDLNKENSNGLGLYIVSNILNNY"
BASE COUNT          271 a           42 c           88 g          206 t
ORIGIN      
        1 gatttggaat ttaaaaataa agaaatttta aaattagaaa agttaaaata tgactttttt
       61 aaaggtgcat cccatgaact taaaacccct cttgctagtc ttaaaataat actggaaaac
      121 atgaaataca atattggaaa atataaagaa agagatattt atattaatga atgtattgaa
      181 atcgttgata gtttaacaaa aaatatttct caaatattat cagttcattc tatagaaaat
      241 ttgaataatg atgaagaata tttgaaaata aatgatacat tagaagacat attaaaaaag
      301 tatgaaatat tagcaaatca aaagaaaata actgtcaata attatatatt agacgagaat
      361 gtttatatag gaaaaacagc tttaaagatt attctatcta atttgattag taatgcggta
      421 aaatatactg atgtaaatgg ggtaattaat attgggatag ttaatgattg gttatatata
      481 gagaattcat acggtaacaa taaaatttcg aatatggata aaatatttga tgttaaattt
      541 gatttaaata aggaaaatag taatggatta ggtttatata ttgtaagtaa tattctgaat
      601 aattaca
//
LOCUS       DAZKSU010000208          590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000208 DAZKSU010000000
VERSION     DAZKSU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.223"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>590
                     /locus_tag="ACG8Z3_002874"
     CDS             <1..>590
                     /locus_tag="ACG8Z3_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0825099.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIRCSA
                     RTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNE"
BASE COUNT          193 a          107 c          120 g          170 t
ORIGIN      
        1 caaatcaaac tgtggtagac gcccgcttgt tttacctgag gaacaatcag aatatattca
       61 aagaaaggtt gttcaaggat ggacacccga tgtgattgtt ggtcgtgcag cgtttcctat
      121 tcgttgttct gctcgtacca tttatcgtat gttcaaaaag gggctattta atccttctga
      181 cttaccgatg aaaggcaagc gtaaaccaaa tggacatcaa gaaagacgtg gaaaacaagc
      241 tttccgtcgc tctattcatg aacgtgaaaa agattatagc caattctcaa atgagtttgg
      301 tcaccttgaa ggtgacacta tcgtaggttt gaaacataaa agtgctgtaa ttaccttagt
      361 tgagcgatta tcaaaagtta tcatcacatt gaaaccgtgt ggtagacaag cgattgatat
      421 tgaaaacaaa ttaaatcatt ggtttgaatc tgtaccgaaa aatctattca aatccatcac
      481 ttttgattgt ggaaaggaat tttcaaattg gaaacagatc agtaatgcca atgatattgc
      541 catttatttc gctgatccag gaacgccgtc tcaaagaggc ctaaacgaga
//
LOCUS       DAZKSU010000209          541 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000209 DAZKSU010000000
VERSION     DAZKSU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.224"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>541)
                     /locus_tag="ACG8Z3_002875"
     CDS             complement(<1..>541)
                     /locus_tag="ACG8Z3_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0825100.1"
                     /translation="FLLVKWIAPFLLSFAEKVYANSSIIIVSLVICLGMSYVADLVGL
                     SSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFVSVGLEVDFSDFSEQLLFI
                     SILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRGEMALIILQIGQQSQLIEP
                     QYYSPLVIVILLSTLISPLV"
BASE COUNT          209 a          111 c           87 g          134 t
ORIGIN      
        1 gaacaagtgg agaaataagt gtacttaata aaatcacgat aactaaaggt gaatagtact
       61 gtggttcgat caattgactt tgctgtccaa tttgtaaaat gatcaaagcc atttctccac
      121 gcgaaatcat accagcaccg accatcaatg aactatgttg agaaaaaccg gcaatctgag
      181 caccaaaata acctccaagc aatttggtca aaatcgctac aagcgtcaat atagaaataa
      241 agagtagttg ttcactaaag tcagaaaaat caacttctaa tcctacactg acaaaaaata
      301 ctggtataaa tactgcatat cctaaagcct ctacattgtg gtatacttct tctttaacct
      361 ttgtctgact gacagctatc ccagcaaaaa aagcaccaat gacagagcta agcccaacaa
      421 gatccgctac gtaagacatt cctaaacaaa taactagcga tacgatgata atagaagaat
      481 ttgcgtacac tttttctgca aatgaaagta ggaaaggcgc aatccatttg actaataaga
      541 a
//
LOCUS       DAZKSU010000210          499 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000210 DAZKSU010000000
VERSION     DAZKSU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.225"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..389)
                     /locus_tag="ACG8Z3_002876"
     CDS             complement(<1..389)
                     /locus_tag="ACG8Z3_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0825101.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFT"
BASE COUNT          147 a          114 c           88 g          150 t
ORIGIN      
        1 gtgaagtatg caacacctgc taaccctttg ccagaaccag caacatattc accatagcaa
       61 gccgcattga catctgttgt ccattcaatc ggaatattga aatattgctt tagcgttcct
      121 acaaaattga aattttgcca agcaagtttt ggtgttgatg tgatatgacc gaaactcgtt
      181 gatttgcatt gaatatcaat tggaccaaag cagccgaccc cgatgcctgc caattgttcc
      241 ttaaaaggtt taaaaaaatc aatgacctgc ttcattgttt caactggagt tgttgtggga
      301 aaactgactc tttccaaaat cgttaattcc tcagtaccga ctgcacagac aaatttagtc
      361 cctccagctt caattgcccc atatatcatc ataggttcct cccttcgaat atgtgaaaaa
      421 cacttcatta acgcggcaag cgttctcgat cagtgaaatg acgtttagta tttcctgaag
      481 cttctgttga ataccagta
//
LOCUS       DAZKSU010000211          490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000211 DAZKSU010000000
VERSION     DAZKSU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.226"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>490)
                     /locus_tag="ACG8Z3_002877"
     CDS             complement(<1..>490)
                     /locus_tag="ACG8Z3_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825102.1"
                     /translation="YVFEKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAF
                     SLLTFYPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGR
                     YNYKLSVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGK
                     PFF"
BASE COUNT          187 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa cttattaaga aagttgaaat
       61 ttttatattc aggaaaccat ttattaattt catgtgcagc acgtaatgca ttccctggag
      121 ttgtcaaaga gaaaattaat tctgctaagc ttattattga aaatactgat aacttataat
      181 tatatcgtcc attgacaatc aaatataaac taactatact tgttagaaca aaaaaacagg
      241 cgttgacttg ttcttgatta gttaaaaata tcaataaagg aatacttacc caatatattt
      301 ttctatttat ttcttctcct cttaagagtt gaaaaaacgg ataaaaagtt agtaaactaa
      361 atgctactgg ccactgatag ttcaaggtgg ttgctctcca accggtttcc cccattactg
      421 tcaatggtat taaacagtat atagagcaaa ccaataataa atttttaaca tttattttct
      481 caaaaacata
//
LOCUS       DAZKSU010000212          481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000212 DAZKSU010000000
VERSION     DAZKSU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.227"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..398
                     /locus_tag="ACG8Z3_002878"
     CDS             141..398
                     /locus_tag="ACG8Z3_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825103.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          206 a           63 c           68 g          144 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 t
//
LOCUS       DAZKSU010000213          478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000213 DAZKSU010000000
VERSION     DAZKSU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.228"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          184 a           59 c           64 g          171 t
ORIGIN      
        1 taatttatac taaattaaat aaagtactac ctttaatagg tagtacttta ttatcaagcc
       61 attattcctt cttctactag ttctttttgt cacataattt tgcaatcaat aaatgttgcg
      121 caataagtat aagaattcca ttttctaaat ttgaaaggtg atccataatt tcatgttcaa
      181 gtagtttatt aattgtccac ttgtcgcttc atctaaaaat tttataggtt ttaggaatgc
      241 tattcgctta ttttaggaag attttaactg tttgctgtag atatatatca tactagtatc
      301 gtaataaaag tactctaaaa gaagaatttt caaatacgca aaattaagta cgaaagagtt
      361 gaaacaaatt tggagaaaaa tgagcataga atgtctaatg agttaaatag acctaaaaat
      421 tatctaaaag tggagcaaaa ttaggaatta caaacaaaac actaaattag tttatata
//
LOCUS       DAZKSU010000214          477 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000214 DAZKSU010000000
VERSION     DAZKSU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.229"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..>477
                     /locus_tag="ACG8Z3_002879"
     CDS             73..>477
                     /locus_tag="ACG8Z3_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0825104.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMS"
BASE COUNT          180 a           90 c           77 g          130 t
ORIGIN      
        1 catacaaaaa aggaagtccc ttctgtagaa taaagttaac gacaaccaat tcacagaaaa
       61 gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt cactaaaggc
      121 gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact cctacggact
      181 gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa ttcaggtaat
      241 tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt aacattggaa
      301 atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta caaaagaaca
      361 aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac gatgtctgaa
      421 attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac catgtcc
//
LOCUS       DAZKSU010000215          450 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000215 DAZKSU010000000
VERSION     DAZKSU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.230"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>450
                     /locus_tag="ACG8Z3_002880"
     CDS             <1..>450
                     /locus_tag="ACG8Z3_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0825105.1"
                     /translation="GKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIV
                     GIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKE
                     FSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFR"
BASE COUNT          147 a           91 c           82 g          130 t
ORIGIN      
        1 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
       61 atccatgatc gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaaggt
      121 gatacgatcg ttggcattca tcataaaagt gccgtcatta ctttagttga aagattatct
      181 aaagtcatta tcacgattaa acccaacggc cgtaaggcat tagatattga aactgccctt
      241 aatcaatggt tttctcgctt ccctaaaaac ttctttaaat ctattacgtt tgactgtgga
      301 aaagaatttt ctaactggaa agccattagt aaccaacatg atattgatat atattttgcg
      361 gaccctggaa caccttctca acgcccatta aacgagaatt ctaacgggat tctgcgtcgt
      421 aatggactgc cgaaatcaat ggattttaga
//
LOCUS       DAZKSU010000216          428 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000216 DAZKSU010000000
VERSION     DAZKSU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.231"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>428
                     /locus_tag="ACG8Z3_002881"
     CDS             82..>428
                     /locus_tag="ACG8Z3_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0825106.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHV"
BASE COUNT          148 a           74 c           86 g          120 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgt
//
LOCUS       DAZKSU010000217          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000217 DAZKSU010000000
VERSION     DAZKSU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.232"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>415
                     /locus_tag="ACG8Z3_002882"
     CDS             <1..>415
                     /locus_tag="ACG8Z3_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0825107.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANSK
                     EEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYD"
BASE COUNT          148 a           75 c           78 g          114 t
ORIGIN      
        1 attgccattg gtatacgaga agacggcact aaagaagtac tgagttatgc gattgctcca
       61 actgaatcaa catacgtttg gaatgagctg ctacaggata ttaactccag aggagttcaa
      121 gaagtcttgc tttttattac ggacggctta aaaggcatga aagatactat ccatcaaatt
      181 tatcctaaag caaaatatca gcattgttgt atccatgtat ctcgtaacat cgctcataaa
      241 gtacgtgtca aagaccgaaa agaaatctgt gatgacttta aggctgttta tcaagctaac
      301 tcaaaagaag aagcgaatac cttcttatcc ggcatgattg agaaatggaa gaaaaactat
      361 cctaaagtga cgcagtcact catagaaaac caagacttat taacttttta tgatt
//
LOCUS       DAZKSU010000218          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000218 DAZKSU010000000
VERSION     DAZKSU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.233"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          171 a           55 c           69 g          116 t
ORIGIN      
        1 gttctaaatg attcctttaa ggtgtcacta aaaattataa aaaatagtgt tgacgcactt
       61 ttgatgtcat gttataataa cgaagtcgtt tggatgagat agctaataaa gcttgatttt
      121 atcgaaaaaa acttcgaaaa agttattgac aaataacaat aaagatgcta tgataatcaa
      181 gtcgctgcta atgagtggca aagacgttta gaaaaaataa acaaagaagt tgttgacatt
      241 ctcttctgaa tctgataaga ttaaagagtt gttaaaacaa aaagattaga cctttgaaaa
      301 ctgaacaaag taagacaaac caaatgtgta gggcgtcttg attcaattca agacaacaaa
      361 catttttaac aagcaagcaa tatgctagca aacaaattga gcttaacaat c
//
LOCUS       DAZKSU010000219          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000219 DAZKSU010000000
VERSION     DAZKSU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.234"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            187..>386
                     /locus_tag="ACG8Z3_002883"
     CDS             187..>386
                     /locus_tag="ACG8Z3_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFQ0825108.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLS"
BASE COUNT          165 a           57 c           51 g          113 t
ORIGIN      
        1 atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac gcagataaat
       61 aaatacatta gattaattcc taccagtgac taatcttatg actttttaaa cagataacta
      121 aaattacaaa caaatcgttt aacttctgta tttgtttata gatgtaatca cttcaggagt
      181 gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc
      241 aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca
      301 ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa
      361 ttagacagtc atctattcaa cttatc
//
LOCUS       DAZKSU010000220          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000220 DAZKSU010000000
VERSION     DAZKSU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.235"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          112 a           65 c           56 g          110 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt tct
//
LOCUS       DAZKSU010000221          322 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000221 DAZKSU010000000
VERSION     DAZKSU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.236"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG8Z3_002884"
     CDS             complement(<1..132)
                     /locus_tag="ACG8Z3_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0825109.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          112 a           60 c           60 g           90 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aa
//
LOCUS       DAZKSU010000222          289 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000222 DAZKSU010000000
VERSION     DAZKSU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.denovo.237"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          134 a           35 c           44 g           76 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactga
//
LOCUS       DAZKSU010000223        14338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.067, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000223 DAZKSU010000000
VERSION     DAZKSU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.067"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..544)
                     /locus_tag="ACG8Z3_002885"
     CDS             complement(101..544)
                     /locus_tag="ACG8Z3_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0825110.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKIAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(739..2079)
                     /locus_tag="ACG8Z3_002886"
     CDS             complement(739..2079)
                     /locus_tag="ACG8Z3_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0825111.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2361..3257
                     /locus_tag="ACG8Z3_002887"
     CDS             2361..3257
                     /locus_tag="ACG8Z3_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0825112.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3400..4341)
                     /locus_tag="ACG8Z3_002888"
     CDS             complement(3400..4341)
                     /locus_tag="ACG8Z3_002888"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0825113.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4467..5588
                     /locus_tag="ACG8Z3_002889"
     CDS             4467..5588
                     /locus_tag="ACG8Z3_002889"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0825114.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5846..7348
                     /gene="eat(A)"
                     /locus_tag="ACG8Z3_002890"
     CDS             5846..7348
                     /gene="eat(A)"
                     /locus_tag="ACG8Z3_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0825115.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7415..7942
                     /locus_tag="ACG8Z3_002891"
     CDS             7415..7942
                     /locus_tag="ACG8Z3_002891"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0825116.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQALAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELISYE"
     gene            8154..9215
                     /locus_tag="ACG8Z3_002892"
     CDS             8154..9215
                     /locus_tag="ACG8Z3_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0825117.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9218..10114
                     /gene="murQ"
                     /locus_tag="ACG8Z3_002893"
     CDS             9218..10114
                     /gene="murQ"
                     /locus_tag="ACG8Z3_002893"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0825118.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            10128..12110
                     /locus_tag="ACG8Z3_002894"
     CDS             10128..12110
                     /locus_tag="ACG8Z3_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0825119.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12180..12626)
                     /gene="nrdI"
                     /locus_tag="ACG8Z3_002895"
     CDS             complement(12180..12626)
                     /gene="nrdI"
                     /locus_tag="ACG8Z3_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0825120.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            12837..14261
                     /locus_tag="ACG8Z3_002896"
     CDS             12837..14261
                     /locus_tag="ACG8Z3_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0825121.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         4624 a         2591 c         3033 g         4090 t
ORIGIN      
        1 cacaacctct gattcacggt gttccagaag tagcttcttc ttatttcttg aagttgatca
       61 cttctggaac atcctctttt tttatttgga tggataatgg tcattccatt tctttgatca
      121 tttttttcat tttgtcgatt tcttcttctt gtgcctgaat gatttcttcc gctatttttt
      181 ttacttcagg atcttttaaa tcagctcgtt tacttgtcaa gatagcaata gagtgatgag
      241 ggatcatcgc cttcatccac gccgtatcgt tgacagttgt ttggcttcgt accataaata
      301 aggaaccggc aaaaacaaga agactagcgg ctaaaatcat agcatttatc tttttgtttt
      361 catacatttt ccacatgaac aacaacatga tgactgccat gactgcaccc atcatcaacg
      421 ccatgaatac acgtgtgcga ctgaagaaga tatgatcaag cgcataaaca ttcaaataca
      481 tcataccaaa catgactacc gtcgaaacac caatcatcaa caaaaacttc caatactttt
      541 tcatttaaaa caaactcctt tttcttcatt cctttcttct atcatgtcat gagcgtggag
      601 aacaatcaat taatctgcac agatggtttt tgaacaccgt ttattcggaa aaaagaactg
      661 caacaatttt tgtcgcagct cctttttttc aatagctcca gatattcaat ataaatagaa
      721 taaaattctc tattggtttc attcattcgt caagacgaaa gcatcattct ttttttgata
      781 actgaaataa atgaacaggt aaagcaaacc ggtcaataca gttaccccaa tggagttcgt
      841 gaataggata ccaaggaaaa tcaaccataa gactgctaat gcaaaaaagc tgattccttt
      901 gtcccataac gttcctttcc ttagtgtcag tacaaaggac gcagcactga taatgatcca
      961 gatcaataat actgtatagg aaagagaacc tgccagatag ccaaataatt tgtcaccgac
     1021 aaaataagaa agagccacac ctagataaag aacactggta caaaacagca cagcacgttg
     1081 cggtacttga tgcttgttta aaacagctag ttttctagag gaacctttcc gatctttcaa
     1141 gcggaagaac aaaattctcg aagaagcata tacacctgaa ttgatagacg agaacagagc
     1201 aagaacaatc acgaaattga cgatatctgc cgcaaatgga atattcattt tttcaaatac
     1261 catcacaaaa ggactaacgt tcgtgcctgc taaagtattc caaggaaaaa taatcaaaag
     1321 caaaaataaa ggaataatgt aaaaggaaat gattcttccc atgacgcctt tgattgcttt
     1381 aggaatcgca cgtttaggat catctgcttc gcttactgtg atagcaatca actcagaacc
     1441 gccgtaagaa tagataacta caagcaaaga actgatgatt cccttcatac cgtggggagt
     1501 aaaaccacca tgatcagtta attgttgaag ggtagggaaa acaccagtac ccaacatttg
     1561 ctgaccgaca agataagcag caaaaatgat caataggata atgacactga ttttagcaaa
     1621 ggccagccaa tattctgtct ctgcgaataa tcgtaccgaa taaagattga tgatagtagt
     1681 taaaacagcc aacataaaaa caaataccca agctgggatg tgggggaacc acaactgtaa
     1741 aaaggaagca gctgcaacag cttcagcaat gatattgata atccacatgg accagtagac
     1801 ccaatcggta aaatcggcag catgagcacc tagatatgga cgaatcaatc cagataatcc
     1861 gtggtgcgta tcaccaccac ttaaaacaat ttttcccaga cctttcatca cgatatataa
     1921 aattatgccg ccaatcaaaa aagcgatcaa tacagaaggg ccggcgattc caatcgcttc
     1981 gccacttcct ttaaatagac ctgcaccaat cgctccacct aaagctagca tggtgatatg
     2041 tcgggagcta agttgttttt tcaatttttt atcttccata aataacctct ttcttttaaa
     2101 ttcacaatca aaatgcatag taagaataaa tagtaagaat aaatagtaat aataataatg
     2161 actaatgata acaaattttc agataattac aactattcag aaaacttttg ggcatatttt
     2221 aatggaagaa tgatttattt catcgtattt tcatagaaat gttaaatttt tataaaaata
     2281 ataggaagcg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
     2341 gagaaagtga ggcggaacag atgaacaaaa acgacgtatt gctaattgcg aaaaataaat
     2401 cactttattt ttttccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
     2461 agtcggaatt cattaaatca gctcgacaac agttggcaaa ttatcagctg atcgtttttt
     2521 tagattatgg atttgatatg gagatggctc gacgcgtgcg tccatttacc aatgcaaaaa
     2581 tcgtgttatt tttttggaac cactttaaag cagaacacga ggcgatgctg aagcaggcac
     2641 aagatgaacc ggcaatcgat gagatttatc attttgattt tttagaagcg aaagaacttg
     2701 gactaaaaca taatagttcc ttttattcga aacatatgga tttttcttct agcgaaccaa
     2761 agacggatct attttttggt gcaacaaata acggcagaaa agaacgggca gaaaaatata
     2821 agcaggaatt tacaaaaaga ggtgttacga ccaattactt tattcttccg cttcgaggaa
     2881 atgaacaacc tggatacctt acttatgaag aatatctgaa aaaaaccggg gaatcgaggg
     2941 ggattctaga actgttgcgg gaagggcagc aaggagtgac tttgcgaacg tttgaatcta
     3001 tgtattttca aaagaaacta gtgacagata atcaagccat agctcattat cagttttatc
     3061 attctgaaaa tatttttttg cttcaagaac gtgatttaga tgaattaccg gaatttttgc
     3121 atacgccatt tcaaccaatc ccatcagaac tgttggaatt ttttgatgca gaaaattggg
     3181 caaaacgttt tttcactact gactccgcta tttatgaaca atatgaatac gatctttcct
     3241 ctgttaaaaa taaataaaag tgccaaccaa tcatagatga aaataaacaa gcgcaattct
     3301 tatggcaaca tgagaattgt gtttttcttt ttgaactttc atgggggtaa aaaaatgaga
     3361 accagctgct gatccagctg attctcatca gagagccttt tattctgcgc caaggattcc
     3421 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     3481 ttgtcctttt cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     3541 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     3601 agcaatatgt tggtacactt cttcttgatc taatgtgatt ccttcttttg ctgcgataga
     3661 agcaaactca gatacaatcg ttcgaaccat gccctctgcc gctttttgcg cacctaattc
     3721 agcaatatta caatccaata tcgtgcataa tccatttaat gttccgttta cacaagcttt
     3781 tcgccaaatg gaataacgaa cattatgact atagattgga ttcaattttg cttcttgaaa
     3841 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     3901 ttctccatca cctaataatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     3961 gatcccaaat aagatatttt tctttggtac atatttttcc agtacatctt catgacctaa
     4021 tccattcagt aaacacaaaa cataagtatt ctcagtaata atggattgga tcgaacgaaa
     4081 catatcatct aattgattcg cttttgtcaa ggctacgatc aaatccacgt gtttgttgct
     4141 ttcgatgatt tcttctggtg aatagattgg cagttttgca actacttctt tgccattaaa
     4201 gtcagcaatc aatccatttg tacgaatggc ttcaatgtga gcaggccagc gatcgatcaa
     4261 gacgacctca tttccgcttt gatgcaacat taatccgatt cggctgccca ttgctccggc
     4321 tccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     4381 tatagaaaaa cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt
     4441 acttaagaag aaagtgaggg atatagatgg atattttggc ggcaatcgat tcatttaaag
     4501 gaagtgcttc ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag
     4561 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     4621 acgcgctcgg gggaacctgg cgtacgattg atgggatgga tctcttattt cgtcagcgaa
     4681 aaatcagtta tttattgacg aactggcaag gaaaaaatac cgcagtcatt gaatctgcac
     4741 aagtattagg attagattta cttgataaac caacggatca aaccatccga cttgcttcta
     4801 gttatgcctt gggagagctt atcaaagata cactgcttca agaagtggaa caaatcattg
     4861 tcactttagg aggaagcgga tgtagcgatg gtggtctagg attactccaa tctttgggtg
     4921 cttctttaga agggtacaca gaaggaaatc ctttgttatc tactagaaaa cttcatttgg
     4981 atcgaaggaa cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc
     5041 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaacggata
     5101 cgacgttagc tttcttggat aaacaagctg aaaagattgc tcagcagata aaagatgttt
     5161 gggaggtgga tttagatgac atcccaggaa gcggagcagc aggcggtatt ggcggagccc
     5221 tttttttgtt aggagcgaag atggtctctg gttttgagtt ggtcagccaa atcactggat
     5281 tagaaaaaca gatcgaacaa gcggatgtca tcattacagg agaagggcgg attgatcagc
     5341 aaacaatcca cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac
     5401 caataatcgc tatttgcgga agtagagacc gagagctgga aggactcttt gattttctcc
     5461 ccattgtctt aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc
     5521 aaacattaga aaaaacagaa atcttaggac agacactcag cagatttttc aacattctcc
     5581 cgaagtagtt gacaaaaaca atttctttat gctatcgtaa tttcaaatat aggacaaagt
     5641 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     5701 tgtggagaca agggagcagt gtgcagattt tcgggattgg cttggttctt tcgatgtatg
     5761 ggtctttttg tgtgccccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     5821 tcttttttta cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg
     5881 ctatgacagc caaggcacat tattatttga acaagccaat ctaaactttg acacacaatg
     5941 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atatcctgca
     6001 aaacaaactc ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca
     6061 gcaaacaaaa gataaagagc gtttgaccta ttatgtgtta aatgagatta tggattttga
     6121 gatatgggaa attgaaagag agctccaact gatgcaaaca gatcctgaaa tcttatggag
     6181 agaattcagc acactatcag gaggagagaa gacaaaagtc ctactggcac tcttatttgt
     6241 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggcag
     6301 aaaacaagta gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga
     6361 tcggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaga gccaactgga
     6421 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     6481 aatagctaaa aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga
     6541 aaaagcagaa tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag tcggattcat
     6601 cgatacggaa tctaggcgag tgaataaagg agcagtaggc gctgatgctg cacggacgat
     6661 gaaacgatct aaagcaatcg tgaatcggat ggaaacccag atcagtgaga aagaaaaatt
     6721 attaaaagat atcgagtata tcgatgcttt gacgatgaat agccaagtgt ctcatcataa
     6781 acggctgtta agcgtagaaa atcttcaatt agggtatgaa aatctgttat tcgagccagt
     6841 tcattttacg atcgaacctc atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa
     6901 gtcatccatt atccattatc ttctggggac attcaacggc aaggttgtag gagaaaaatc
     6961 ccagcccaaa catttgacca ttagttacgt acgccaaaat tatgaagaca atcgagggac
     7021 attggcggaa ttcgcgaaga aaaaccgagt agactatcaa gcatttttga ataatctccg
     7081 aaagcttggg atggaaagag atgtttttca taataaaatc gaacagatga gtatgggaca
     7141 acggaaaaaa gtggaattgg ctaaatcttt atcccaacca gctgagctct atatatggga
     7201 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa
     7261 cgtgaaacct gctatgttac tagtggaaca tgatcaaacc tttctagata aagtatctac
     7321 tgagattatt tctcttgaga aaatctaaac cttgtaatct caaagacaac aaggtatggt
     7381 taaggagaac tttgtcaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     7441 aacaacttgg aacgcacaag gattagtttg cgggcgagca gatgtgccgc tgactgaaaa
     7501 aggacagatg caggcacagg cactagcaga aaaggtagta gatttgcctg ttccaatcac
     7561 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag tagttgatcg
     7621 tctcaatttg ccgatgaccg ctgatgagcg attggtagaa atggattttg gggattatga
     7681 cggactgcca agcaaagatg aaaactttca aaaagcgcgt ttagcgtttg ctgtacgttt
     7741 cccaaatgga gagtcggtct tagatgtgta cgcacggatc gttcctttat tgaaagaatg
     7801 catggaagac gaagaaaatg tctacttgtt agtctgtcat aatgccttga taagagtgat
     7861 caatgcttat tttcatccaa tgcctaatga agggttcttt acattcatgg tagacaatac
     7921 agaattaatt tcttatgagt aattagactt ttgtgttatt taaataaaat gtttcaaggc
     7981 actgcttttg cttagcagtg ccttttttgt ggctgtgtca attcttttgg cattataaat
     8041 ccaattttta gaaataaaat ttaattattt taacaaaagt attgaaattt tgtttcttta
     8101 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     8161 gcttttcaat atttatgaat gattctttgt cagaagagaa gaaacagtat atccgccaaa
     8221 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccggaa gatgatgttt
     8281 cggcttatca aaatcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagagt
     8341 tgatggtcga tatttcagga gaggcattga aacgagctgg cttttcatta gaagatctaa
     8401 cacccttgaa agcaattggt gtaacagggt taagaatgga ttatcatatt tctaatcagc
     8461 agatcgccga ctggtcacat caattgaaaa tcagtttgaa tgccagcacg atcacgccta
     8521 atgatatcga tgagttgaaa gaagcagcgg ctgatttctc acagattgaa gcttggcata
     8581 attattatcc acgtcttgaa acagggttgg acaaagaatg gtatcaggaa aaaaatcaat
     8641 ggctcaaaac tcaaggattc cttgtacaag gctttgtacc aggagataca gagttgagag
     8701 gtccactgta tcaaggatta ccaactttgg aagaacatcg agggatccat cctctggcag
     8761 gtgctcttga tctattagca agtaacacgg atattgttta tatcggagat gcaggtctgt
     8821 cagaacacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     8881 cggaacctgt agatgaagtt ttttacgaat atatattagg aaaacatacg aatcggcaag
     8941 atgatgcaag agatgtcata cgaagcgcag atgcgcgttt tcgagagatt ccgccaatcc
     9001 cagtaagaaa tacagctaag cgtatgaaag gatctattac tttagataac gaaaagtatt
     9061 tgcgctatat gggtgagatc caattaacta aatatgattt gccagcggat gaaaaagtga
     9121 atgtagtagc caaagtcatc aaagaagaac taccgctgat caaccaaatc aaagctggta
     9181 tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa
     9241 cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgaaatga gtatttctca
     9301 agcagtccaa ctcatgaaca aagaagatca gaaagtggcc gctgctgttg aaaaagaact
     9361 gccaagtatc gagccagtga tcgccaacac gattgaatcc tttaaaaaag gtggtcgctt
     9421 gatttatatg ggagctggaa cgagtggtag acttggtgta ttagatgctg ctgaatgtgt
     9481 accgacattc ggtgtagaac cggaaatggt cataggccta attgctggtg gagaagaagc
     9541 aatgactgta gccgtcgaag gagccgaaga ttcttatgaa ttagggcaag aagatctaat
     9601 caagctgaat ttaacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc
     9661 atatgtgatt ggtggattag attacgcaaa atcgattggt gctgttacag gaacgatttc
     9721 ttgcaataaa caagcagaaa tcagcgagca tgcggatttc ccaatcgaag tcgatgcagg
     9781 accagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa aactgatttt
     9841 gaatatgatt tctactatca gtatgatcgg tatcggtaaa gtattcaata acttgatggt
     9901 cgacgtcaaa ccaacaaatg aaaaactaat tgaacgctca aaacgaatca tcatgcaagc
     9961 gacagaagct gactacgaaa cagctgaaaa gtactttgag gaagcagatc aaaatgtcaa
    10021 actggctatc gtcatgattt tgacaaatag tgataaagag gcagctcaag caaaattagc
    10081 tgaagcaaac ggttttgtaa aaggaacctt atgaggggga aaagaccatg gcaaaaaacg
    10141 aattgacttt ggaagaactg gcaaaacaaa tttatcaagg agcaggcggt atgggcaatg
    10201 ttgaaagcat tgtccactgc atgactcgtg tacgtatgaa ggtcatcaat gatgatcttg
    10261 taaatgtctc ggaattaaaa gcgattccag gagtacttgg cgtagtagag gatgaacagc
    10321 tccaagtcat catcggacct gggaaagtga acaaagtagc gcaggaaatg gttgacttag
    10381 caggcgtgaa attaggagaa aaacttcctt ctgctagtgc aactacagca agttcttctg
    10441 catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga
    10501 aacaaaaacc ctctaaaatc aaatcggtat taaaagacat ctctaatatc tttgtaccaa
    10561 tgatccctgc atttgtcggc actgggatcg tggcagggat tgccgcagtt ttagcaaact
    10621 tagtcacagc tggaacacta gacgcaggaa catggcaaca atatatcgat gtgatgaaca
    10681 tcttgaaaaa cgggatgttt agctatctag ttatctatac aggtatcaat gctgcacaag
    10741 tttttggtgc aacgccaacg cttggtggag ttatcggggc tgttgtgatg ctgacaggga
    10801 tgaatccaga agcaccactg aaaaacttgt ttaccggtga agctcttgca actggacaag
    10861 gcgggatctt aggtgttatc ttcgcagttt ggttattgtc aatcgttgag aaaaaactgc
    10921 ataaagtagt acctgatgcg gtagatatca tcgttacgcc aactctttcc ctgattgcca
    10981 tcggtctgat cgaaattttc ttcatcatgc ctttagcagg gttcatttct gacggcatgg
    11041 tcggtgggat caactgggta cttggtgttg gaggagcatt tgccggcttc gttctaggta
    11101 cattattctt gccaatggtc atgttcggac tacatcagat cttgacgcca atccatgtac
    11161 aaatgattga tgaaactggt cgtaccttat tgcttccaat ccttgcgatg gccggagcag
    11221 gacaagtggg tgcagcattg gcattatgga tccgctgcaa aaaagacaaa gaattaacag
    11281 aaatgatcaa aggggcttta ccagtcggaa tcttaggaat cggcgaacca ttgatttacg
    11341 gagtaacatt acctctagga cgtccattta ttactgcttg tatcggtgga ggtatcggtg
    11401 gggcagtgat cggaatgatc ggaaatgtcg gagcaatcgc tattggacct tcaggagccg
    11461 ctttgattcc attgatctct gatggtaaat ggttaggcta tatccttggt ttattggctg
    11521 cttacgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa
    11581 aagaagaact agcgaccgaa acagccacag taaatgaagt acctgaaaaa gcagtacctc
    11641 aagcttcagc tgaaagtgtg actatcttat catcagttgc tgacggaaca gttgtttctt
    11701 tagataaagc aagtgaccca gtttttgcac aaaaaatgat gggtgaaggt tactttgtag
    11761 aaccagaaaa cggacatatc tattccccgg ttactggagt aataagttct gttttcccaa
    11821 caaaacatgc tatcggcatt acgacaccaa atggcttaga aatcttgctt cacatgggca
    11881 tcaacacagt tgacttaggc ggtacgccat tcgatgtgaa agtaacagaa ggacagcaag
    11941 tgacacctga tacgcttgta gcagaagctg acttagaagc aatcaaagca gcagacaaag
    12001 aaacctccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa
    12061 aaacaggtaa agcgaaagca aaagcacctg tgatggatgt caaagcttaa taagaataga
    12121 aacaatagag aacataaaaa gaagaagccg aaatttattt ttcagcttct tcttttgtgt
    12181 tatttatttt tttctgccaa aatttgataa acgcgtgtca catcagatgg tgttccccgt
    12241 aattcataat ctgccaagaa aggaaagccg aatctttgtg cgtattgttt ggctgtcaga
    12301 caatattgat tgttgaagtt tttattaccg ctaccgacta caccaaggca aagtttatga
    12361 ttgtcttcgt attccagata ttcgcgcatc gtttcggtca agatttctgt gtcaccgttg
    12421 tctaacccat tcccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct
    12481 ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct
    12541 tctgcatgca ttttttcagc atattctgct agatgtttgg caaaagcacg cgtatttcct
    12601 gaaatcgata tatataaaat gttcatggat catcccccaa ttctttccct ttagtatagc
    12661 atctttttaa gtttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata
    12721 atgagaaaaa agtcggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat
    12781 aaaaaaggtt tgttatactt tttgataaaa gcggttttac tttggaggat gcttacatgg
    12841 atacagagca acagaaaaaa ggaattggtt tgattccact tgctgcctta gtgattggtt
    12901 cagcgattgg aggtggcgta tttggtatta tgacggacat ctccggatcg gctggtggcc
    12961 cagctatctt cagctgggta ctcgtaggaa cagcaatgtt gatgctctcc ttatcgatca
    13021 ataatatcaa taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag
    13081 gttttggccg tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag
    13141 gaaacgtggc gttcgcaaca ttgttgatga gtgcattagg ttatttcttc cccgtattcg
    13201 aaggtggtca aaatgtggca tcgatcgtga tgagtacgat cttcttatgg ggatatgcat
    13261 ggctagtgaa tcgtggagtt gaaaatgcta gctttgtcaa tgctatcgtc acattttgta
    13321 aattagtgcc attattttta tttattgtag tagctatcag caccttttcg atccaacaat
    13381 tcattaataa cttttctagc gatatcatat tgaatgcgaa tgggcagccg atcccgtttt
    13441 ctagtcaagt attgagttgc atcatggtga tgctctgggt attcgttggt attgaaggag
    13501 cttctgtcgt aggttcacga gcaaaaaaac aatcggatgt tgggaaagca acgattctag
    13561 gaatattcgg tttgatcatt atttacgtct tggtttccat gcttccatat ggcacaatga
    13621 cgatgaatca gctgcaaacg atcaaagcgc aacctgctat gggatatgta tttgaaatga
    13681 tggttggtaa atggggggcg gtcgtgatca acggcggttt gatcatttct ttagttgggg
    13741 tttggctctc atggacaatc ttgccgattg aaacaatggg gaatatggct gaggacggac
    13801 ttttgcctaa agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat
    13861 tgacgacgtt atgtacgaat gtctttttac tttcattatt attcactgac agtgcatata
    13921 actttgctta tacacttggt acagcagcga ttttcttcac gtggcttttc ttagggctgt
    13981 accaaatgaa gctttcttat cagcggaaag agtgggtcca atttttagta gggtttttag
    14041 caagtgcttt tcaagtatgg gccatgctag ttgtagcctt taatgaagtg atgcttgttc
    14101 ttgttctttt cttaccaggt atttattttt acctcaaagc acgtaaagaa caaaacgaac
    14161 ctgtacaaca tatccactta gataagattt tacttatcgg tgtgacgatg gtcggagtaa
    14221 tcgcaatggt tttatttgcc actgggatgc ttcacgtata gaagtataaa gagaggctgt
    14281 gaagcctgtg gtttattcaa aaaaggctct ttgtcaaatg atgttggtaa agagaaat
//
LOCUS       DAZKSU010000224         7780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.101, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000224 DAZKSU010000000
VERSION     DAZKSU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.101"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            245..1213
                     /locus_tag="ACG8Z3_002897"
     CDS             245..1213
                     /locus_tag="ACG8Z3_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0825122.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="ACG8Z3_002898"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="ACG8Z3_002898"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0825123.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEGGCA
                     VLGNSAALAGGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="ACG8Z3_002899"
     CDS             2243..2851
                     /locus_tag="ACG8Z3_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0825124.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /locus_tag="ACG8Z3_002900"
     CDS             3279..4190
                     /locus_tag="ACG8Z3_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0825125.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ-A"
                     /locus_tag="ACG8Z3_002901"
     CDS             4343..4828
                     /gene="vanZ-A"
                     /locus_tag="ACG8Z3_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0825126.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5115..5444)
                     /locus_tag="ACG8Z3_002902"
     CDS             complement(5115..5444)
                     /locus_tag="ACG8Z3_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0825127.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5749..6078
                     /locus_tag="ACG8Z3_002903"
     CDS             5749..6078
                     /locus_tag="ACG8Z3_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0825128.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6068..6355
                     /locus_tag="ACG8Z3_002904"
     CDS             6068..6355
                     /locus_tag="ACG8Z3_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0825129.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(6634..7197)
                     /locus_tag="ACG8Z3_002905"
     CDS             complement(6634..7197)
                     /locus_tag="ACG8Z3_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0825130.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2567 a         1298 c         1549 g         2366 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgagggc gggtgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctggtg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacca tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aactttaaaa aacacgaata gcggaaagga tgtcttcatt cgctaaagag
     5161 taataaacta cttttccatc ttgacgtgat tttgccattc catgtttttt taaaaaacgt
     5221 aaatggtgtg acgttgtagc gacactagca gtaagtattt ctgctaaatc acagacgcac
     5281 atttccttat aggtttctaa agcatagaaa attttaattc tagaaaaatc gctgaaacat
     5341 ttccctaaca caagtaagtt tgaaaaatct ttttgatcca atttattttt tattccattt
     5401 actttttctt tatgtacgac agtcacttgg caaacttttt tcattcatct cacctcacat
     5461 tcaaacgatt atttgaatga agtataccta tttgatcact cttttgtcaa tctaccttaa
     5521 ttccaataca ctattataag caatttcatg tattgaagta aacttacttg caagtgcgtt
     5581 tttgattcca agttaaaaac atattattat tctacataat ttcctaatca aataaaactc
     5641 ctttgaaaat tgttctaaag gagttttatt attttgtatt tctactaaaa atagcgtaca
     5701 atgtaattac taagtaatta cgaaatgtaa aataatggag gaaataatat gattacaaaa
     5761 acaagaaaac aaggaaattc tattatgtta acggtcccta aagaattcga tgtgcccaat
     5821 ggtgttgaag tagaagctaa gcttgtagag aatggtatcc tttatgagtt tgtagagcct
     5881 aaaaaagaat tttttgattt tagtgaagat attttggctg atattctttc agaaggttat
     5941 agcaaacaag agatcttaaa agaatttaga aatagaaaaa atgcgttaac ttctgcattt
     6001 aaatctattg ctgaagatac tgttgcaaat tctaaaccga tgacaaaaga ggagttggca
     6061 gcagaaattg gcttatgaga ttctaccttc taaacacgta ataaagtatc taaaaaaatt
     6121 aaaagagaaa acattaaaag aacaattttt aacgataatt tatgatgaga tagcagttcg
     6181 tcctcattcg ggagaacaaa aaacgggtga tttatcagga atttgggcta ttggattcaa
     6241 gtatgcaggt acgacatatc gcgttgcata tgaaattaaa gataatgcag tgatacctaa
     6301 cttactatgt ggaacgcatg aaaattttta tgaacaatta aaaaagatta ggtaagaacc
     6361 caatgtttca acttgagagg atagacgaca ctttaaaaga taatttttaa cagctgagag
     6421 ttgtaatacc ctataatttt aaaccactaa cctatatatt tatgaaaaat agaaaaaatt
     6481 aaaacgcatt ttaagcctct ttcaaattaa aagcttcatc cttatgcaca aagagggctt
     6541 aaaatgcgtt attttatatt gttttttagt aataataaaa aaatcactct aattctccca
     6601 taaatattat agattcctaa agattaaaat agattattcc ctattgtctt ttaagtagcg
     6661 gtagagtgtt gccttgctaa taccagtcat ttccgtaatt tcttttacgc tataaacctg
     6721 acttttatac aattttactg ctcgatcaac atctttctgg ttcactcgtg gacggccacc
     6781 tttacgacct ctcgcacgtg cacttttcaa accttctttg gtacgttcaa caattaaatc
     6841 acgttcgaac tgactaaatg cttggaagac agtcatcatt aagcgtccgt gaggcgtgct
     6901 ggtatcgaag ttttctttca agctcaccag cttaacatgg tgttcttcaa aataggtaac
     6961 aaggtcaatc aaatcctttg tactacgacc caatcgagaa aaactttcta caattaccgt
     7021 atctcccgga cgaattttat cttttaaacg atttagttgg ggacgaaccg ctttggttcc
     7081 cgtcattttc tcagttagga tttcattaca attttgttca ttgagtaagt ctaattgacg
     7141 atttagttct tgtgaacgtg tactcactcg ggcataacca taaatgtatc cactcatgct
     7201 tttcttcctt tcgtacttta attctggctt gattgtatca taaaagggag ttatagatac
     7261 atagttaatg caacaagtta tgatacaaaa tatgacctct tttatttaat aaaagaggtc
     7321 atttcatttt gtctcataat cgattgtttt tgatacgagt gtgtatacga aataaacaaa
     7381 aaaatcttaa gtaaatttct agatacctaa tttaatccta actagtgtat cctaaagtta
     7441 ataacgagtt tatcacaatg aaaattataa aatgctccca tattagatat aacatgcaat
     7501 atttgaatta gaactacaaa atttattcgt tttataaagt aaataatgtt gagatactaa
     7561 ggaatagaat attataagga attgagctga atgtattgga taaaaaaaga ataactttat
     7621 tagagatgga ctataaaaat gctcgtaatt ttcttctgaa gtctgaaagc tatttgaatg
     7681 ctcagctacc agaatatatt agttttgacg aaactttaaa atctgctaaa aaagttttaa
     7741 cttcgccgtt tgtataatta agcgagacaa ataaaaaagc
//
LOCUS       DAZKSU010000225         7780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.102, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000225 DAZKSU010000000
VERSION     DAZKSU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.102"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            245..1213
                     /locus_tag="ACG8Z3_002906"
     CDS             245..1213
                     /locus_tag="ACG8Z3_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0825131.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="ACG8Z3_002907"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="ACG8Z3_002907"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0825132.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="ACG8Z3_002908"
     CDS             2243..2851
                     /locus_tag="ACG8Z3_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0825133.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            3279..4190
                     /locus_tag="ACG8Z3_002909"
     CDS             3279..4190
                     /locus_tag="ACG8Z3_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0825134.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            4343..4828
                     /gene="vanZ-A"
                     /locus_tag="ACG8Z3_002910"
     CDS             4343..4828
                     /gene="vanZ-A"
                     /locus_tag="ACG8Z3_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0825135.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5115..5444)
                     /locus_tag="ACG8Z3_002911"
     CDS             complement(5115..5444)
                     /locus_tag="ACG8Z3_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFQ0825136.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            5749..6078
                     /locus_tag="ACG8Z3_002912"
     CDS             5749..6078
                     /locus_tag="ACG8Z3_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0825137.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            6068..6355
                     /locus_tag="ACG8Z3_002913"
     CDS             6068..6355
                     /locus_tag="ACG8Z3_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0825138.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(6634..7197)
                     /locus_tag="ACG8Z3_002914"
     CDS             complement(6634..7197)
                     /locus_tag="ACG8Z3_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0825139.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         2567 a         1298 c         1546 g         2369 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttttat
     3301 tgttattgtt attcttaata tacttaggtt atgactacgt taatgaagca ctgttttctc
     3361 aggaaaaagt cgaatttcaa aattatgatc aaaatcccaa agaacattta gaaaatagtg
     3421 ggacttctga aaatacccaa gagaaaacaa ttacagaaga acaggtttat caaggaaatc
     3481 tgctattaat caatagtaaa tatcctgttc gccaagaaag tgtgaagtca gatatcgtga
     3541 atttatctaa acatgacgaa ttaataaatg gatacgggtt gcttgatagt aatatttata
     3601 tgtcaaaaga aatagcacaa aaattttcag agatggtcaa tgatgctgta aagggtggcg
     3661 ttagtcattt tattattaat agtggctatc gagactttga tgagcaaagt gtgctttacc
     3721 aagaaatggg ggctgagtat gccttaccag caggttatag tgagcataat tcaggtttat
     3781 cactagatgt aggatcaagc ttgacgaaaa tggaacgagc ccctgaagga aagtggatag
     3841 aagaaaatgc ttggaaatac gggttcattt tacgttatcc agaggacaaa acagagttaa
     3901 caggaattca atatgaacca tggcatattc gctatgttgg tttaccacat agtgcgatta
     3961 tgaaagaaaa gaatttcgtt ctcgaggaat atatggatta cctaaaagaa gaaaaaacca
     4021 tttctgttag tgtaaatggg gaaaaatatg agatctttta ttatcctgtt actaaaaata
     4081 ccaccattca tgtgccgact aatcttcgtt atgagatatc aggaaacaat atagacggtg
     4141 taattgtgac agtgtttccc ggatcaacac atactaattc aaggaggtaa ggatggcgga
     4201 atgaaaccaa cgaaattaat gaacagcatt attgtactag cacttttggg gtaacgttag
     4261 ctttttaatt taaaacccac gttaactagg acattgctat actaatgata caacttaaac
     4321 aaaagaatta gaggaaatta tattgggaaa aatattatct agaggattgc tagctttata
     4381 tttagtgaca ctaatctggt tagtgttatt caaattacaa tacaatattt tatcagtatt
     4441 taattatcat caaagaagtc ttaacttgac tccatttact gctactggga atttcagaga
     4501 gatgatagat aatgttataa tctttattcc atttggcttg cttttgaatg tcaattttaa
     4561 agaaatcgga tttttaccta agtttgcttt tgtactggtt ttaagtctta cttttgaaat
     4621 aattcaattt atcttcgcta ttggagcgac agacataaca gatgtaatta caaatactgt
     4681 tggaggcttt cttggactga aattatatgg tttaagcaat aagcatatga atcaaaaaaa
     4741 attagacaga gttattattt ttgtaggtat acttttgctc gtattattgc tcgtttaccg
     4801 tacccattta agaataaatt acgtgtaaga tgtctaaatc aagcaatctg atctttcata
     4861 cacataaaga tattgaatga attggattag atggaaaacg ggatgtgggg aaactcgccc
     4921 gtaggtgtga agtgagggga aaaccggtga taaagtaaaa agcttaccta acactatagt
     4981 aacaaagaaa gcccaattat caattttagt gctgaggaat tggtctcttt aataaatttc
     5041 cttaacgttg taaatccgca ttttcctgac ggtaccccca aactaagatt tcatagatag
     5101 attttctgat aactttaaaa aacacgaata gcggaaagga tgtcttcatt cgctaaagag
     5161 taataaacta cttttccatc ttgacgtgat tttgccattc catgtttttt taaaaaacgt
     5221 aaatggtgtg acgttgtagc gacactagca gtaagtattt ctgctaaatc acagacgcac
     5281 atttccttat aggtttctaa agcatagaaa attttaattc tagaaaaatc gctgaaacat
     5341 ttccctaaca caagtaagtt tgaaaaatct ttttgatcca atttattttt tattccattt
     5401 actttttctt tatgtacgac agtcacttgg caaacttttt tcattcatct cacctcacat
     5461 tcaaacgatt atttgaatga agtataccta tttgatcact cttttgtcaa tctaccttaa
     5521 ttccaataca ctattataag caatttcatg tattgaagta aacttacttg caagtgcgtt
     5581 tttgattcca agttaaaaac atattattat tctacataat ttcctaatca aataaaactc
     5641 ctttgaaaat tgttctaaag gagttttatt attttgtatt tctactaaaa atagcgtaca
     5701 atgtaattac taagtaatta cgaaatgtaa aataatggag gaaataatat gattacaaaa
     5761 acaagaaaac aaggaaattc tattatgtta acggtcccta aagaattcga tgtgcccaat
     5821 ggtgttgaag tagaagctaa gcttgtagag aatggtatcc tttatgagtt tgtagagcct
     5881 aaaaaagaat tttttgattt tagtgaagat attttggctg atattctttc agaaggttat
     5941 agcaaacaag agatcttaaa agaatttaga aatagaaaaa atgcgttaac ttctgcattt
     6001 aaatctattg ctgaagatac tgttgcaaat tctaaaccga tgacaaaaga ggagttggca
     6061 gcagaaattg gcttatgaga ttctaccttc taaacacgta ataaagtatc taaaaaaatt
     6121 aaaagagaaa acattaaaag aacaattttt aacgataatt tatgatgaga tagcagttcg
     6181 tcctcattcg ggagaacaaa aaacgggtga tttatcagga atttgggcta ttggattcaa
     6241 gtatgcaggt acgacatatc gcgttgcata tgaaattaaa gataatgcag tgatacctaa
     6301 cttactatgt ggaacgcatg aaaattttta tgaacaatta aaaaagatta ggtaagaacc
     6361 caatgtttca acttgagagg atagacgaca ctttaaaaga taatttttaa cagctgagag
     6421 ttgtaatacc ctataatttt aaaccactaa cctatatatt tatgaaaaat agaaaaaatt
     6481 aaaacgcatt ttaagcctct ttcaaattaa aagcttcatc cttatgcaca aagagggctt
     6541 aaaatgcgtt attttatatt gttttttagt aataataaaa aaatcactct aattctccca
     6601 taaatattat agattcctaa agattaaaat agattattcc ctattgtctt ttaagtagcg
     6661 gtagagtgtt gccttgctaa taccagtcat ttccgtaatt tcttttacgc tataaacctg
     6721 acttttatac aattttactg ctcgatcaac atctttctgg ttcactcgtg gacggccacc
     6781 tttacgacct ctcgcacgtg cacttttcaa accttctttg gtacgttcaa caattaaatc
     6841 acgttcgaac tgactaaatg cttggaagac agtcatcatt aagcgtccgt gaggcgtgct
     6901 ggtatcgaag ttttctttca agctcaccag cttaacatgg tgttcttcaa aataggtaac
     6961 aaggtcaatc aaatcctttg tactacgacc caatcgagaa aaactttcta caattaccgt
     7021 atctcccgga cgaattttat cttttaaacg atttagttgg ggacgaaccg ctttggttcc
     7081 cgtcattttc tcagttagga tttcattaca attttgttca ttgagtaagt ctaattgacg
     7141 atttagttct tgtgaacgtg tactcactcg ggcataacca taaatgtatc cactcatgct
     7201 tttcttcctt tcgtacttta attctggctt gattgtatca taaaagggag ttatagatac
     7261 atagttaatg caacaagtta tgatacaaaa tatgacctct tttatttaat aaaagaggtc
     7321 atttcatttt gtctcataat cgattgtttt tgatacgagt gtgtatacga aataaacaaa
     7381 aaaatcttaa gtaaatttct agatacctaa tttaatccta actagtgtat cctaaagtta
     7441 ataacgagtt tatcacaatg aaaattataa aatgctccca tattagatat aacatgcaat
     7501 atttgaatta gaactacaaa atttattcgt tttataaagt aaataatgtt gagatactaa
     7561 ggaatagaat attataagga attgagctga atgtattgga taaaaaaaga ataactttat
     7621 tagagatgga ctataaaaat gctcgtaatt ttcttctgaa gtctgaaagc tatttgaatg
     7681 ctcagctacc agaatatatt agttttgacg aaactttaaa atctgctaaa aaagttttaa
     7741 cttcgccgtt tgtataatta agcgagacaa ataaaaaagc
//
LOCUS       DAZKSU010000226         5445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.121, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000226 DAZKSU010000000
VERSION     DAZKSU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.121"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1740
                     /locus_tag="ACG8Z3_002915"
     CDS             <1..1740
                     /locus_tag="ACG8Z3_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0825140.1"
                     /translation="AKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVP
                     IDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELP
                     TVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAV
                     GVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPN
                     HPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHE
                     VKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRN
                     ISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAG
                     IHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFL
                     PMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDEL
                     FEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQ
                     EETSETLPDDNDM"
     gene            1970..3037
                     /gene="rlmN"
                     /locus_tag="ACG8Z3_002916"
     CDS             1970..3037
                     /gene="rlmN"
                     /locus_tag="ACG8Z3_002916"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0825141.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            3027..3233
                     /locus_tag="ACG8Z3_002917"
     CDS             3027..3233
                     /locus_tag="ACG8Z3_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825142.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(3230..4333)
                     /locus_tag="ACG8Z3_002918"
     CDS             complement(3230..4333)
                     /locus_tag="ACG8Z3_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0825143.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            4886..5434
                     /gene="aac(6')"
                     /locus_tag="ACG8Z3_002919"
     CDS             4886..5434
                     /gene="aac(6')"
                     /locus_tag="ACG8Z3_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0825144.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1815 a          949 c         1163 g         1518 t
ORIGIN      
        1 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
       61 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
      121 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
      181 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
      241 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
      301 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
      361 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
      421 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
      481 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
      541 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
      601 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
      661 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
      721 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
      781 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
      841 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
      901 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
      961 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     1021 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     1081 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     1141 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     1201 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     1261 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     1321 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     1381 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     1441 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     1501 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     1561 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     1621 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     1681 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     1741 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     1801 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     1861 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     1921 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     1981 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     2041 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     2101 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     2161 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     2221 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     2281 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     2341 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     2401 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     2461 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     2521 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     2581 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     2641 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     2701 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     2761 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     2821 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     2881 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     2941 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     3001 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     3061 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     3121 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     3181 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     3241 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     3301 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     3361 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     3421 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     3481 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     3541 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     3601 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     3661 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     3721 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     3781 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     3841 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     3901 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     3961 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     4021 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     4081 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     4141 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     4201 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     4261 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     4321 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     4381 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     4441 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     4501 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     4561 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     4621 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     4681 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     4741 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     4801 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     4861 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     4921 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     4981 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     5041 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     5101 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     5161 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     5221 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     5281 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     5341 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     5401 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggt
//
LOCUS       DAZKSU010000227         5013 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.123, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000227 DAZKSU010000000
VERSION     DAZKSU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.123"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1130
                     /locus_tag="ACG8Z3_002920"
     CDS             <1..1130
                     /locus_tag="ACG8Z3_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HFQ0825145.1"
                     /translation="LAYIRLYSGVLHLRDSVRVSEKEKIKVTEMYTSINGELCKIDRA
                     YSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALL
                     EISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERP
                     LKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIR
                     YGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYL
                     SFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTN
                     GLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            1228..1416
                     /locus_tag="ACG8Z3_002921"
     CDS             1228..1416
                     /locus_tag="ACG8Z3_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0825146.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1474..1827)
                     /locus_tag="ACG8Z3_002922"
     CDS             complement(1474..1827)
                     /locus_tag="ACG8Z3_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0825147.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2358..2780
                     /locus_tag="ACG8Z3_002923"
     CDS             2358..2780
                     /locus_tag="ACG8Z3_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0825148.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2777..3007
                     /locus_tag="ACG8Z3_002924"
     CDS             2777..3007
                     /locus_tag="ACG8Z3_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0825149.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3505..3705
                     /locus_tag="ACG8Z3_002925"
     CDS             3505..3705
                     /locus_tag="ACG8Z3_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFQ0825150.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3732..4925
                     /locus_tag="ACG8Z3_002926"
     CDS             3732..4925
                     /locus_tag="ACG8Z3_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0825151.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         1728 a          808 c          926 g         1551 t
ORIGIN      
        1 tacttgcata tatacgcctt tatagtggag tactacattt acgagattcg gttagagtat
       61 cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt gaattatgta
      121 agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt ttgaagttaa
      181 atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa aatccgcacc
      241 ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg ttgcttgatg
      301 cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat tctacgacac
      361 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
      421 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
      481 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
      541 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
      601 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
      661 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtacg
      721 gtttatacta tagccctgtt agtactccag cagattttcg gatgcttact cctattgtac
      781 tggagcaagc ctttagaaaa gctggaacag aattgttaga gccatatctt agttttaaag
      841 tttatgcacc acaggaatat ctttcacggg catataacga tgctcccaaa tattgtgcaa
      901 atatcgtaaa tactcaactg aaaaataatg aggtcattat tattggagaa attcctgctc
      961 gatgtattca agattatcgc aatgatttaa ctttttttac aaatgggctt agtgtttgtt
     1021 tagcagagct aaaaggatat caggttacca ctggcgaacc tgtttgccag acccgtcgtc
     1081 taaatagtcg gatagataaa gtaagatata tgttcaataa aataacttag tgcgttttat
     1141 gttgttatat aaatatggtt tcttattaaa taagatgaaa tattctttaa tatagatttg
     1201 aattaaagtg gaaaggagga gattgttatt ataaactaca agtggatatt gtgtcctatt
     1261 tgtggaaata aaacaagact acgaatacga gtggatacta tacttaaaaa tttcccttta
     1321 tactgcccca aatgtaagaa cgaaacttta attaatgttc aaaaaatgaa tataataaca
     1381 atcaaagagc cagacgccaa gacgcagagc cgataatttg agaaatgaaa ctctcatctt
     1441 atcggctctt tttgtttatc tgaattttac tgactagcct tcaatatttc ttgcttcatt
     1501 gatacctttt gctagtgctt ccattaagga tagttctttg tctgtaaagc tatccatgta
     1561 tttttctacc tgtaaccttc gagtgctttt aatcaaatta tctgtaggta agaaaaattc
     1621 atcaatagat acatggagta atgatacaag atcatacagc acttgtgtac ttgggtgttg
     1681 tcctttgttt tctatatttg ttaaataacg tgggtcaatt tcaatcattg ttcccacttg
     1741 ttcacgagtg agaccccgtt tcattcgagc ctctttaatc gctaaaccaa cggctctaaa
     1801 atcgtattta tcttcttttt tacgcatact aaatcacctc tatacatttt actgttccta
     1861 tataaccata aacaggtata gaaaaacatg ttatatagtt tatgagttcc tatagaacac
     1921 tgtggaattt attaatgcga tgaatccaat ttcaaataga aaggtcgctg attttattta
     1981 cctatacaac tttataaaat ttagtcatac tcctagaaag tagataaaat tggcgactta
     2041 atggagggaa aagtcgccat gagaaagata atctgtcatc tagttctctt aattaagttc
     2101 ttatcaaaaa aacttatcca cctgtactgg atagatatga ccatcaatgt ggttttgcag
     2161 attacataga tagaaaaatc aaagcatatg aacagagaaa acaatctgtt tatgtgcttt
     2221 tttggtcact taataaatat cttttaggat ttctcttcat ttatgcaaca aatcccccgt
     2281 tcgtgtcggg gtttagtatg aagggagata aagaagcaca tgccactccc tttcacgaca
     2341 gaaagggggt gaaaagcatg aaaccatctt cttttcaaga aaccatagaa aatcagtttg
     2401 actatatctg caaacaagtt attgaagatg aacgaaaaga ttacttcaaa catctagaca
     2461 gactatcagc aaaagaagtc tcgttttctg atatagataa ctaccttgtc aactcttttt
     2521 ctactgttga tacgtacgtc actgatttcc aactatttaa attatttgga ttaagaatca
     2581 atgttgaaag tgatttatta agtgaagccc tgcgtcatct ttcagagaaa aaacggaaca
     2641 ttattttact tcattacttt atggatatga gtgatgtgga gattgcggag ttactaaatt
     2701 tgaatcgttc gactgtttat cgtcatcgaa taagtggact agccatgatt aaagaattca
     2761 tgaaggagtg taaagaatga aaacagctta tccaagggtt cccttccccc tcattgtaaa
     2821 ggcgacagat ggcgacgtgg aagcaattaa tcagattgtt aaacattatc gagggtatac
     2881 ctcaaaacgt tcattacgtc gcatgacaga cgaatatgga aattctcata tggttattga
     2941 tgaaacccta cgtggcagaa tggaaacgaa actcatctct aagattttat cgttcgaaat
     3001 taaatagtcc cattttttcg tggaagcgtg tcgaatcgta tccacgcttc ccgatttggg
     3061 aggtttgtta ttccatcaaa gcatattgtg tgttcagtag gttttgatag gctaacaagc
     3121 caaacgttct ttgacaacca aataaaggca atcgaatacg tttcggtaag aaaagagctg
     3181 atgaactaac acgccatgac ctacaaaatt agtgagcgat tcatgttagt aattcgaaag
     3241 taatctttag caggattgct gccatgactt tcttatcgtg ataatgatac tcccgtatag
     3301 ccatagtccg agcgttagaa gcgtcacagg caatgggtac ggctacatga gaaccatgca
     3361 gaagtgaaat tcctgtgagc tttgctagag ctgttcgatt gctaagatat tcttctcttg
     3421 tcagtgtatt attataaaac tatttgatat aattataggt gaatattgga gttttcctag
     3481 cctataattg ggaaaggagt aataatgata acggtcacaa aaaaggattt agttgagcta
     3541 ggatatggaa cttctttttc agcagacatc ataaaaaaag caaaagaatt gatgattaca
     3601 aaagggcata tctactatca atctcgaaag ctagatagag tccctcgtga agctgttgaa
     3661 gaaattctag gaattcggtt ttcaaatgaa aacaggtctg agtagttttt gcacagtaag
     3721 gagtaaaaac tatggttaaa gacccaataa aaaaagcaaa gaatggaacc tattattttc
     3781 gtgcaaatct aggttatgat tctaaaggga aaaaaattca aaaatatcgt agtggattca
     3841 ctactaaaaa agaagcaaga gaagaatatt cgaaactgct attgatgaaa ccagaagaat
     3901 taagtgagaa taaggataaa atgacgttta aacattatat ctttgaaatt ttccttccat
     3961 ggtataagac acaggtaaag ctcagaacat atgaaaatcg cttaccaact attaagaagc
     4021 atttctctta ttttgataaa atggctgttt ccgatattga gcctattgat gtgcaaaatt
     4081 ggcaactaaa attatcaaag aagtgtaagt cttcttatgt aagagctgtt caaggactat
     4141 tttcagttgc tatggatcga gcaattgtcc ttgggataac caccacaaat ccatctaaga
     4201 ttattggcaa tgtgaaaaag caaaagtcta aaattgaatt ttggacaaaa gaagaatttg
     4261 aaaaagtaat ctctctaatt tacaaagaag attactatca acacttttta tttatctctc
     4321 tgtggttttt atttatgact ggaatgcgaa ttggtgaagc tactgccatt caatgggaag
     4381 atattgattt tgactcagga gtactaacca tcaacaaaac actctattac aagaatcaat
     4441 cgaattatag atttgttgaa ccaaaaacaa aggcgagcgt tcgtcatatt gtattagata
     4501 aatgcacatt aacttattta tccgaatgga aggaagtaca acaaaatcta attcagacag
     4561 actttgtaat gagctataac ggtattccta cccaaaaaca tacactatca tatgctattg
     4621 aacgctatgc taagctggct ggtatccatc gaatcagaat ccatgggtta cggcattctc
     4681 atgcttcctt gttaatcagt atgggagaaa atccacttat cattaaagat cgtctaggac
     4741 acgaagatat tgagacgact ttaggaacgt atggacatct ttatcctaac agtaatttcg
     4801 aggtagctaa caaattaaat ggagccattt cctttaaaga agcaaaacaa aattatgata
     4861 cttcaccaaa gaatcaattt actgtagact acttgcgaaa caaagaaatg aaaaaagtgc
     4921 aataaaagtg cagtaaaaag ataagaagag ccagaaaccc ttggtataag aggatttcgg
     4981 ctctataatt tatgggactg atgaatcaga att
//
LOCUS       DAZKSU010000228         4325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.133, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000228 DAZKSU010000000
VERSION     DAZKSU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.133"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            276..1754
                     /gene="msr(C)"
                     /locus_tag="ACG8Z3_002927"
     CDS             276..1754
                     /gene="msr(C)"
                     /locus_tag="ACG8Z3_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0825152.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1801..2247
                     /locus_tag="ACG8Z3_002928"
     CDS             1801..2247
                     /locus_tag="ACG8Z3_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0825153.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2446..2961)
                     /locus_tag="ACG8Z3_002929"
     CDS             complement(2446..2961)
                     /locus_tag="ACG8Z3_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0825154.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3078..4279
                     /locus_tag="ACG8Z3_002930"
                     /pseudo
     CDS             3078..4279
                     /locus_tag="ACG8Z3_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         1299 a          728 c          934 g         1364 t
ORIGIN      
        1 aaatgactgc atcgatgaaa ttacgtttcg aacttttgaa taacaactaa ataaattcaa
       61 aagacatgta taaacaacaa tttcatggat ggagtatgtc cattttttcg aacgacagta
      121 gaataggtga aagctatgac agatttggtg atttagtcat agttatttcg ttcagagtag
      181 gaaaagatgt gatgcagttt ttttgcaaac agatttttct gctctttttt agtatcgata
      241 attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca gtaaatataa
      301 caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct cttcgtgtct
      361 atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg actttgctca
      421 aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag atcaccttca
      481 actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg gaattagcca
      541 gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga ggggaagaac
      601 gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt ctagacgagc
      661 caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt cgttattatt
      721 atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta gcatcgaaaa
      781 tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt gcctatctca
      841 ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc atgaaagaga
      901 aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag
      961 tgtccagtaa aaagaaaaag caacaaatca gaccggatcg gttgtcttcc tctaaacaaa
     1021 aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga agactccaaa
     1081 aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat
     1141 ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg gaaagaagtg
     1201 ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa atcgcgattg
     1261 tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa caaggagaag
     1321 gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt tatcaaatga
     1381 catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca gaatcgcttg
     1441 tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta
     1501 cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact aagccaagta
     1561 atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc gaagctttag
     1621 agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat tcttactttg
     1681 tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa aaagtactta
     1741 cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga gtggtacaga
     1801 ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt tttgatggtc
     1861 ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc aagtttgttg
     1921 attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat catcggtgtg
     1981 ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt ccaaatgatc
     2041 ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt attcggtgtg
     2101 atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc ttggaaaatg
     2161 aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc aagaagtgtg
     2221 ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa ggccttggca
     2281 tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg aacgataggt
     2341 tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt tattgaagat
     2401 aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact caaaaaactg
     2461 ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa gcataattct
     2521 gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga ggtcattcaa
     2581 ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga atagcagtcc
     2641 agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa attcatcttt
     2701 ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac gtccttcatc
     2761 agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg tcacataacc
     2821 ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt cccaaaaaat
     2881 tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca cgcataataa
     2941 tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt tattatatca
     3001 cagtaaagtt tccacgtttt acatgtgcat atttacatgt aaaataaatc atgtatgatg
     3061 ctaatagaga gaaagggatg gaaatattgg agaatcaaat agatatacaa gaacaaacaa
     3121 atgaagaaac aaaggggaaa agttcagcgc tagttgtagc agctacctgt atgatcgctt
     3181 ttatgggagt gggtctagta gacccaatct taaaaacgat tgctgcacag ttaaacgcta
     3241 cgccagctga aacgaccctg ttatttacta gttacatgtt agtcactggt gtggtgatgc
     3301 tatttacagg tttcttatct agtcggatcg gtttgaaaaa acattgatga caggattgtt
     3361 tatcatcgtt gtattcgctg gtcttggcgg attatctggg aatatcggaa tgctgattgg
     3421 gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc tctactgctt tagcaacgat
     3481 cgtcagtatt ttagttggta acacagaaaa agcaatcatg atgtatgaag cagcacttgg
     3541 ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg ggcagtggtt catggcgtta
     3601 tccatttttt ggggtagcat ttttgatgtt ttgcggtttt atagccctag cggtactttt
     3661 acctgaaact gagaaaccaa agagaaaagt aggcctttgg gaaggtgtca aagcgttaag
     3721 caatcataaa cttcgttcag ttggactaat cgcattgtta tataatttcg gattctttac
     3781 tttacttgcg tatgcgccat ttttattgat tggctattct gaattagaag tcggttttgt
     3841 attttttggc tggggtgtgc ttctggctat tgcttcgatt ttcgttgcac cggctcttga
     3901 gcggaagttt acgacttatc ggacaatttt agcagcattt gtcttattca ttatttgttt
     3961 gctgttatta ggagctggaa cttctatgcc agtccttgta ccgatcagca ttgtgttagc
     4021 aggatttttc caaggaatta tcaatacact gttgacgacg attgctatgg aaataccagg
     4081 tctgcaacga aacgttgctt cgtccagcta tagttttgtc cgctttttcg gtggagcttt
     4141 agcaccattt attgcaggtg tgattggtga acatattggt gaaagctatg cgttttattt
     4201 tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt tatcaccgta attattttgc
     4261 tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     4321 aagaa
//
LOCUS       DAZKSU010000229         4315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.134, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000229 DAZKSU010000000
VERSION     DAZKSU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.134"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..401
                     /locus_tag="ACG8Z3_002931"
     CDS             <1..401
                     /locus_tag="ACG8Z3_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0825155.1"
                     /translation="YLIVDLKLCNRTNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFR
                     IVSCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDM
                     TEKLYNEVNEIVSEINNFLNEESSDERKQI"
     gene            382..1116
                     /locus_tag="ACG8Z3_002932"
     CDS             382..1116
                     /locus_tag="ACG8Z3_002932"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0825156.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1149..2057
                     /locus_tag="ACG8Z3_002933"
     CDS             1149..2057
                     /locus_tag="ACG8Z3_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0825157.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2054..2596
                     /gene="sat4"
                     /locus_tag="ACG8Z3_002934"
     CDS             2054..2596
                     /gene="sat4"
                     /locus_tag="ACG8Z3_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFQ0825158.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2689..3483
                     /locus_tag="ACG8Z3_002935"
                     /note="aph(3')-IIIa"
     CDS             2689..3483
                     /locus_tag="ACG8Z3_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFQ0825159.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            3759..4301
                     /locus_tag="ACG8Z3_002936"
     CDS             3759..4301
                     /locus_tag="ACG8Z3_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0825160.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         1478 a          649 c          980 g         1208 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccaa cttttttatg tttgaagcag
       61 ggttctcttt aatgtttgta aaagcaaatt cgggaacaga cgataaatat tatattgcgg
      121 gtcatgtttt tcgtatagtt tcatgtttaa atcaagtgtt atttgcatgt aataatgctt
      181 attgtatcaa cgaaaagaaa gctataaaac tgcttgaaac ttttgaacat aaacctgaaa
      241 aatataccga gaaggtaaat catatttttg aagtactcgg tatctcactt tttgaatgct
      301 acgacatgac cgagaagctt tataatgaag tgaatgaaat tgtatcggag ataaataact
      361 ttttaaacga ggagagttca gatgaaagaa aacaaatatg atgataatat attttttcaa
      421 aaatacagtc aaatgagtcg ctcgcagaaa ggactggctg gtgcgggaga atgggagact
      481 ttgaaaaaga tgctacctga ttttaagggt aagcgtgtgc ttgatttagg atgcggctat
      541 ggatggcact gtatatatgc gatggaaaac ggtgcttcct ctgtagtagg tgttgatatt
      601 tctcataaaa tgctcgaagt agcaaaagga aaaacccatt ttccacagat tgaatatgaa
      661 tgctgtgcca tagaagatgt ggatttccca gaggagagct ttgatgtaat actaagttcg
      721 cttgcgtttc attatgtagc agactatgag aatttaataa aaaagatata taggatgctg
      781 aaggctggtg gcaatttagt ttttacagtt gaacatcctg tttttactgc tcatggaaca
      841 caagactggt attataacga aaaaggagaa atactgcatt tcccggtgga caattattat
      901 tatgagggca aacggacagc tatgtttttg gaagaaaagg ttacaaaata tcatagaaca
      961 ctgaccacat atctaaatac actgctttca aatagtttta taataaatca gattgtggag
     1021 ccacagccgc cagagaacat gatggatatt ccggggatgg cggatgaaat gcgacgccca
     1081 atgatgctga ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa taaacgagga
     1141 gtatgtaaat gagatcagaa aaagaaatga tggatttagt actttcttta gcagaacagg
     1201 atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag
     1261 atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta
     1321 atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg
     1381 aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata
     1441 aaattgatct taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat
     1501 taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata
     1561 tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt
     1621 ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg
     1681 atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca
     1741 tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat
     1801 ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat
     1861 gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa
     1921 ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt
     1981 ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag
     2041 agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc
     2101 cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac
     2161 ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga
     2221 agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct
     2281 ttactaccaa gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt ggaaccggta
     2341 cgcttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc
     2401 gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga
     2461 aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg
     2521 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
     2581 gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc
     2641 ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg
     2701 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
     2761 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
     2821 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
     2881 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc
     2941 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
     3001 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
     3061 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgattagc cgaattggat
     3121 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca
     3181 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
     3241 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
     3301 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
     3361 gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta
     3421 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
     3481 tagtacctag atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct
     3541 tttctgaagt acatccgcaa ctgtccatac tctgatgttt tatatctttt ctaaaagttc
     3601 gctagataga gttctatatt agagatgtaa agagttttgg tgaagaggtt tgtgagtatg
     3661 gcttttataa ccagggtgca atgagaagca ctaacagcag ctagcttaag aacgcatagg
     3721 atatactttt tatggaagtc acagaggagg aataaaattt gaacaggatt aacagagtaa
     3781 cctctattct tattcagctg caatcaaaaa aaataattcc tgccaaagaa attgcacagc
     3841 gatttaatat aagcttaagg acagtttaca gagatatccg gacacttgaa gaagccggaa
     3901 taccaattgg atctgaggcc ggaaaaggat attttcttgt agagggcttc ctacttccgc
     3961 cggtaatgtt tacagcggcg gaagtgggtg cattgattac agcagggaaa tttttaaatt
     4021 gccatggaga tgaatcattt ataaaggatt ttgattcagc tatgtataaa atcaaatcta
     4081 ttttgaagca tggtgaaaaa aactatgcac aggagttgga gaacagtatt aacgtgtaca
     4141 gcacatctgg acagaaaaat acattggcgg ataacgttat tgcagcaatt cagactgcaa
     4201 tctgcaataa aagggtaata tcaattcaat atcctgcatc aggagggcaa gaaccggaaa
     4261 gcagaatgat agagccggtt ctgttgcaaa gttttaaata aagaataaaa tccct
//
LOCUS       DAZKSU010000230         3720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.138, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000230 DAZKSU010000000
VERSION     DAZKSU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.138"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(531..656)
                     /locus_tag="ACG8Z3_002937"
     CDS             complement(531..656)
                     /locus_tag="ACG8Z3_002937"
                     /inference="COORDINATES: protein motif:HMM:NF019687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825161.1"
                     /translation="MLFPPFKSSKSRKNPSDKYSYHWDFNTKIAVYYTLIATDAL"
     gene            641..2581
                     /gene="tet(S)"
                     /locus_tag="ACG8Z3_002938"
     CDS             641..2581
                     /gene="tet(S)"
                     /locus_tag="ACG8Z3_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HFQ0825162.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            2669..2836
                     /locus_tag="ACG8Z3_002939"
     CDS             2669..2836
                     /locus_tag="ACG8Z3_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0825163.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(3350..3706)
                     /locus_tag="ACG8Z3_002940"
     CDS             complement(3350..3706)
                     /locus_tag="ACG8Z3_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0825164.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
BASE COUNT         1327 a          577 c          618 g         1198 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaacccttt taatgatatt ttccaagtca
       61 aacttaatat taacaattca aaactattgg aaccactgca aaatattttg ttaagcagta
      121 tatatttcaa aaattatacg taatatattt gtagaaaggt tgtggacagt tgtcctattg
      181 acctgaaaag taatataata gctggatgaa taaagaatcc cttatgaact tgtaaatcgt
      241 tattaaatcg ctgaattttg atagccattc tgaagagtta tcttcaacta ctacactaac
      301 ttccattaat ttctcattaa tttccctttc gttcaaaaaa tatggttggc tttacaagca
      361 ttctatcata tgatagaatt aagataacat tcaaaaggag ataataaaaa tgcggcaagg
      421 tattcttaaa taaactgtca attagatagc gggaataatt aattaagcat ccttatttaa
      481 aataaggact ggttttttgt gcccagttta agaatacttt tatcatttta tcataaagca
      541 tctgtggcta tcagtgtgta gtatacagct atttttgtat taaaatccca gtgataagag
      601 tatttatcac tgggattttt tctgcttttt gagcttttga atggaggaaa taaaattgaa
      661 aattattaat atcggtatct tagcacatgt tgatgcagga aaaactactt tgacagaaag
      721 cttactatac agtagcggag caattaaaga gttaggaagt gtagatagcg gtacaacgaa
      781 aacggatact atgtttttgg aacgccagag aggtattact attcagaccg caataacatc
      841 ttttcaacgg gaaaatgtta aagtaaatat tgtagatact cctggacaca tggatttttt
      901 ggcagatgta taccgttcat tatctgtttt ggatggagct attttgctaa tctctgcaaa
      961 agatggagta cagtcacaaa ctcgtatact attccatgca cttagaaaga tgaacatacc
     1021 tataatattt tttattaaca aaattgatca aaatggaata aatttgccag atgtttatca
     1081 agatattaag gacaaacttt ctgacgacat cataattaag cagactgtga atctaaattt
     1141 gaaaccttat gtaatagatt atactgaacc agaacaatgg gagacagtaa ttgtgggaaa
     1201 tgattattta ttagaaaaat ataccattgg gaaaacattg aatattgcag aacttgaaaa
     1261 ggaggaaaac gaaagaattc aaagttgctc cttatatcct gtttatcacg gaagtgcaaa
     1321 gaataatatt ggaattaaac aacttataga ggtaattact agcaaattat tttcacccac
     1381 acaactcaat tcagataaac tttgtggaaa tgtttttaaa gtagaatatt cagatgatgg
     1441 tcaacggctt gtctatgtac gtctttatag tggaacgcta catttgcgag actcagtcaa
     1501 tatatcagaa aaggaaaaaa taaaagttac agaaatgtat acttcaataa atggagaatt
     1561 acgccagata gataaggcag agcctggtga gattattatt ttaaaaaatg agcttttaaa
     1621 actaaataac gtacttggag ataaaaaaag attaccacat agagaaattc ttgagaatcc
     1681 tcttcctatg ttacaaacaa caattgaacc atgtaaatca gtacaaagag aaaagttact
     1741 agatgcactt tttgaaatat ccgatagtga tccccttcta caatattatg tagatacagt
     1801 aactcacgaa attgtgctat cttttttagg tgaggtccaa atggaggtaa cttgtactct
     1861 gattcaagaa aaatatcata ttgagataga aacaagaaaa ccaactgtca tttatatgga
     1921 aagaccatta aaaaaatctg aatttaccat tgatatcgaa gtacctccaa atcctttctg
     1981 ggcttctatt ggtttatctg taacaccact tcctttgggt agtggcattc agtatgagag
     2041 cctggtttct ctaggttatt taaatcaatc atttcaaaat gcagttatgg aaggtatacg
     2101 ctatgggtgt gaacaaggat tgtacggttg gaaattaaca gactgtaaga tctgttttaa
     2161 gtatggtcta tattacagcc ctgtcagtac gccagcagat ttccgaatgc ttgcgcctat
     2221 tgtactagag caggctttta gaaagagtgg tacagagtta ttagagccat atcttagctt
     2281 cgaaatttat gtaccacaag aatatctttc gagagcatat aatgatgctt ccaaatattg
     2341 tgcaaatatt ttaaatacta agttaaaagg taacgaggtc attctcattg gtgaaattcc
     2401 agcccgttgt attcaagagt atcgaaacag tttaactttc tttacaaatg gacgcagtgt
     2461 ctgtttaaca gagttaaaag gttatcaggt tactaacatt aagtctgctt tccaaccacg
     2521 tcgtccaaat aatagaatag acaaagtaag gcatatgttt aataaaatca acttacattg
     2581 atagtgaact aaagagaaat ttttaaaatg aatattgagt tgttaattag aacaggagga
     2641 tatagatatt gtaaatacta ggtggatact gtgccctata tgtggcaata agacccgttt
     2701 aaaaatgagg gaagatactg aattaaaaaa tttccctcta ttttgtccta aatgcagaca
     2761 agaaatttta attgaaataa cgaagtttag aataactgta attacagagc cagacgcaaa
     2821 gacgcagagc cgataattcg agatatcaaa atctcatttt atcggctttt tcttttctat
     2881 gcataccatt gagcttagtt cttgatatcc tttataagaa aataagtgta ctgatttttt
     2941 ttgcagtagc tgtattacag tagacatccc tcaagcataa aaagtataca attaaaattc
     3001 aatacttgat tattgatgaa gtacagagtt actcgtcatt tcaaaactag tatatcagtc
     3061 aagtttttcc taaggaacaa aaacgaactt cgctatggga atcaaaaatt tttagtcgga
     3121 tgaagtgata tgcaataaat ccagaaaatt ttcccaacaa caaaaaatga actgtacttt
     3181 gcagcgataa gagtaaccaa agaactattt atcattcgct ctagttccta agtgtaacaa
     3241 agataaattc attttctatt tatgattaac aaaaatcaga aaaaattcat tacatctttg
     3301 taatcaatct ttcctgatag tattaattaa tccattgtgt attattgaac tagttaaatt
     3361 tacatttttc tccgtaaatt gcttgaatca ctttcccaat cgtcactaat tcatcttcat
     3421 ccagacaatc caactgtgct tctacttgaa gacgtttgga gcttttatga ctgttttctt
     3481 cccctaaaat aaatccatcc aaagaaatat ttagcaaagt cactaaatca tatacaacat
     3541 gaaaacttgg ctgttgtcct ttattttcaa tgttcgtcaa atagcgtggc gcaatctgga
     3601 tcattgatcc tacctgctca cgagtcaatc ctttactaag acgggcttct ttaatcgcaa
     3661 gacccaatgg tttgaaatca aaatttgata gagtagtttg ccatattctt gcacccaacg
//
LOCUS       DAZKSU010000231         2565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.150, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000231 DAZKSU010000000
VERSION     DAZKSU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.150"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..341)
                     /locus_tag="ACG8Z3_002941"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACG8Z3_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACG8Z3_002942"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACG8Z3_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACG8Z3_002943"
     CDS             complement(1055..1345)
                     /locus_tag="ACG8Z3_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0825165.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1553..2244)
                     /locus_tag="ACG8Z3_002944"
                     /pseudo
     CDS             complement(1553..2244)
                     /locus_tag="ACG8Z3_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          759 a          472 c          356 g          978 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac
     1561 gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg
     1621 ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta
     1681 atttcttttg tgtatctgtt aactgatttc tacatttgtt ttttcttgtg tcagtgttgc
     1741 gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg cttgtgcttc
     1801 ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt tttggttgat
     1861 ctcatctagt ttgacattga tttcattttg tttgctttgt agttgtgcat ccttgctgac
     1921 taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt tttctaattc
     1981 atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg tctgttcttt
     2041 ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca atgtatgatc
     2101 aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc acaaaataat
     2161 ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa cgacaatccc
     2221 accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt ttacatgcta
     2281 aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt aattttaata
     2341 taaatactat caaatactca tttaatcctc cttttttatc tatttaaaaa atctttttta
     2401 tagagaaaaa tgcaggttaa tactttctta aataaatatt tatggtgaac aaaaacaaaa
     2461 aagcaagttt tattaaacgt aacccttttc atttcataga ttactgtatt tattacaaaa
     2521 aacgcggatt tgtcataaat tgtcatgctt cttttaccta tgaaa
//
LOCUS       DAZKSU010000232         2030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.164, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000232 DAZKSU010000000
VERSION     DAZKSU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.164"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            152..847
                     /gene="vanR-A"
                     /locus_tag="ACG8Z3_002945"
     CDS             152..847
                     /gene="vanR-A"
                     /locus_tag="ACG8Z3_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0825166.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            825..1979
                     /locus_tag="ACG8Z3_002946"
     CDS             825..1979
                     /locus_tag="ACG8Z3_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0825167.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          672 a          379 c          438 g          541 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa agaatcatca tcttaagaaa
       61 ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg ataaattatt
      121 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      181 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      241 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      301 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      361 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      421 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      481 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      541 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      601 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      661 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      721 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      781 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      841 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      901 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      961 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
     1021 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1081 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1141 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1201 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1261 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1321 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1381 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1441 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1501 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1561 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1621 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1681 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1741 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1801 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1861 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1921 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1981 agatgtatat aattttggct ctttgtcaaa tgatgttggt aaagagaaat
//
LOCUS       DAZKSU010000233         1994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.166, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000233 DAZKSU010000000
VERSION     DAZKSU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.166"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..437
                     /locus_tag="ACG8Z3_002947"
     CDS             45..437
                     /locus_tag="ACG8Z3_002947"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0825168.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            438..1877
                     /locus_tag="ACG8Z3_002948"
     CDS             438..1877
                     /locus_tag="ACG8Z3_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFQ0825169.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          830 a          153 c          342 g          669 t
ORIGIN      
        1 cattgtatag cattttacac aggagtctgg acttgactga gtttatggaa gaagttttaa
       61 ttgatgataa tatggttttt gatattgata atttaaaagg atttcttaat gataccagtt
      121 catttgggtt tatagctaaa gaaaataata aaattatagg atttgcatat tgctatacac
      181 ttttaagacc tgatggaaaa acaatgtttt atttacactc aataggaatg ttacctaact
      241 atcaagacaa aggttatggt tcaaaattat tatcttttat taaggaatat tctaaagaga
      301 ttggttgttc tgaaatgttt ttaataactg ataaaggtaa tcctagagct tgccatgtat
      361 atgaaaaatt aggtggtaaa aatgattata aagatgaaat agtatatgta tatgattatg
      421 aaaaaggtga taaataaatg aatatagttg aaaatgaaat atgtataaga actttaatag
      481 atgatgattt tcctttgatg ttaaaatggt taactgatga aagagtatta gaattttatg
      541 atggtagaga taaaaaatat acattagaat cattaaaaaa acattataca gagccttggg
      601 aagatgaagt ttttagagta attattgaat ataacaatgt tcctattgga tatggacaaa
      661 tatataaaat gtatgatgag ttatatactg attatcatta tccaaaaact gatgagatag
      721 tctatggtat ggatcaattt ataggagagc caaattattg gagtaaagga attggtacaa
      781 gatatattaa attgattttt gaatttttga aaaaagaaag aaatgctaat gcagttattt
      841 tagaccctca taaaaataat ccaagagcaa taagggcata ccaaaaatct ggttttagaa
      901 ttattgaaga tttgccagaa catgaattac acgagggcaa aaaagaagat tgttatttaa
      961 tggaatatag atatgatgat aatgccacaa atgttaaggc aatgaaatat ttaattgagc
     1021 attactttga taatttcaaa gtagatagta ttgaaataat cggtagtggt tatgatagtg
     1081 tggcatattt agttaataat gaatacattt ttaaaacaaa atttagtact aataagaaaa
     1141 aaggttatgc aaaagaaaaa gcaatatata attttttaaa tacaaattta gaaactaatg
     1201 taaaaattcc taatattgaa tattcgtata ttagtgatga attatctata ctaggttata
     1261 aagaaattaa aggaactttt ttaacaccag aaatttattc tactatgtca gaagaagaac
     1321 aaaatttgtt aaaacgagat attgccagtt ttttaagaca aatgcacggt ttagattata
     1381 cagatattag tgaatgtact attgataata aacaaaatgt attagaagag tatatattgt
     1441 tgcgtgaaac tatttataat gatttaactg atatagaaaa agattatata gaaagtttta
     1501 tggaaagact aaatgcaaca acagtttttg agggtaaaaa gtgtttatgc cataatgatt
     1561 ttagttgtaa tcatctattg ttagatggca ataatagatt aactggaata attgattttg
     1621 gagattctgg aattatagat gaatattgtg attttatata cttacttgaa gatagtgaag
     1681 aagaaatagg aacaaatttt ggagaagata tattaagaat gtatggaaat atagatattg
     1741 agaaagcaaa agaatatcaa gatatagttg aagaatatta tcctattgaa actattgttt
     1801 atggaattaa aaatattaaa caggaattta tcgaaaatgg tagaaaagaa atttataaaa
     1861 ggacttataa agattgatta tataatatat gaaaagctat tataaaagac attagtatta
     1921 aatagtttaa aaaaatgaaa aataataaag gaagtgagtc aagtccagac tcctgtgtaa
     1981 aatgctatac aatg
//
LOCUS       DAZKSU010000234         1889 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.168, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000234 DAZKSU010000000
VERSION     DAZKSU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.168"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            245..1213
                     /locus_tag="ACG8Z3_002949"
     CDS             245..1213
                     /locus_tag="ACG8Z3_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0825170.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..>1889
                     /locus_tag="ACG8Z3_002950"
     CDS             1206..>1889
                     /locus_tag="ACG8Z3_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase"
                     /protein_id="HFQ0825171.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEGGCA
                     VLGNSAALAV"
BASE COUNT          562 a          355 c          454 g          518 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgagggc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgtt
//
LOCUS       DAZKSU010000235         1752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.171, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000235 DAZKSU010000000
VERSION     DAZKSU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.171"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..770
                     /gene="dfrG"
                     /locus_tag="ACG8Z3_002951"
     CDS             273..770
                     /gene="dfrG"
                     /locus_tag="ACG8Z3_002951"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0825172.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(842..>1752)
                     /locus_tag="ACG8Z3_002952"
     CDS             complement(842..>1752)
                     /locus_tag="ACG8Z3_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0825173.1"
                     /translation="LNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          589 a          251 c          320 g          592 t
ORIGIN      
        1 agtagttcag ttttggagta caaaatgaca aaatgtactt caaaatggac tacttttttc
       61 attttaactt acataccaaa ttacatacaa aatgatttat aaaatgcaat tgaaaatgat
      121 aattgaatga aaatttgtga ttttgtggta ttatgataat ataacttata gaaaaaattg
      181 cttattcaac taacgggcag gataatgaga aaaggttttt tgatagaaat aagaaattag
      241 atggtaaatt agttaaaaag gctgtgttat aaatgaaagt ttctttgatt gctgcgatgg
      301 ataagaatag agtgattggc aaagagaatg acattccttg gaggattccc aaggactggg
      361 aatatgttaa aaatactaca aagggacatc cgataatatt aggtaggaag aaccttgaat
      421 caatcggaag agccttacct gacagaagaa atattattct gacgagagat aaggggttta
      481 cctttaatgg ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta tgtaaaaacg
      541 aagaagaaat ttttattttc ggaggagaac agatttataa tttgtttttc ccttatgttg
      601 agaaaatgta catcacaaaa atacatcatg aattcgaagg agatactttt tttccagaag
      661 tgaattatga ggaatggaat gaggtatttg cccaaaaagg gataaagaat gataaaaatc
      721 cgtataacta ctattttcat gtatatgaaa gaaaaaactt attgagttaa cgggtgcgtt
      781 aattgaagaa gaacagtaaa gataaagcac aagagcttgg gattactcca agctcttttt
      841 gtcatttgaa ataaattata tctttccaat caaattgttt atctgtaatc cctaatcttt
      901 gagcaggtgt caaaaccttt ttatccgctg atttaaaagg gcgacaaaag ttgtagtaag
      961 ttcttagaat tgtaatcgca aattgagcat acttcggatt gaagttagcg taaatatagc
     1021 tcttcttatc accacgagct gttgttaatg gtctttcaag agatgatata taacgtctta
     1081 tctgttgaat aaagctattg gtcgaatggt cattaacatt taatagcatc tttgcgattt
     1141 cgtttggttc cagagcagat aaatccgttc tacaatcaac tgaataaaat cctttatcct
     1201 tagtggctaa tggatgtttg atagggttat cggcatattt gcgatatctt tctccatctt
     1261 tactaatagc ctcttcgtgg aattgatgac ttgttagtaa ttcagttaga taatgagatg
     1321 ccaaagtccg tagaaacttt gttttgaatc ctctgatatc tccccaatcg agcagttctg
     1381 cttttgcttg tccgaattct tttaagcatt gttttctgct tttcgtcttg ttaacttggc
     1441 aaataaaatg atgtgcatcg ctcaacctta attctttcgt gaatactcta taaaaggcgt
     1501 ttctaataga gaagtcatca tcactaatca ttcgccattc gtcggcatta accatttgtt
     1561 taattaacca atagtgagca gttgtagtat aaggagcatc aacatttaaa ccatcgacaa
     1621 atctactcct attagtaatt ttccctaatt cgtgaagata agcattccta ttttcgctat
     1681 cgttagcgga tggttcttgt ggataataag accaatctaa cctatcattt ttacgactaa
     1741 aagtattgag at
//
LOCUS       DAZKSU010000236          787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.206, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000236 DAZKSU010000000
VERSION     DAZKSU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.206"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..770
                     /gene="erm(B)"
                     /locus_tag="ACG8Z3_002953"
     CDS             33..770
                     /gene="erm(B)"
                     /locus_tag="ACG8Z3_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0825174.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          292 a          138 c          122 g          235 t
ORIGIN      
        1 tttatagatg taatcacttc aggagtgatt acatgaacaa aaatataaaa tattctcaaa
       61 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      121 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      181 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      241 aattaaaact gaacattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      301 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      361 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      421 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      481 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca
      541 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      601 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      661 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
      721 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
      781 cgctttt
//
LOCUS       DAZKSU010000237          787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE2995
            SAMEA115706375-rid22575393.guided.207, whole genome shotgun
            sequence.
ACCESSION   DAZKSU010000237 DAZKSU010000000
VERSION     DAZKSU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706375
            Sequence Read Archive: ERR13196111
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287631.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287631.1
            Genome Coverage       :: 227x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 10:48:23
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,894
            Genes (coding)                    :: 2,820
            CDSs (with protein)               :: 2,820
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 37 of 74
            Pseudo Genes (incomplete)         :: 37 of 74
            Pseudo Genes (internal stop)      :: 19 of 74
            Pseudo Genes (multiple problems)  :: 14 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706375-rid22575393.guided.207"
                     /isolate="VRE2995"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..770
                     /gene="erm(B)"
                     /locus_tag="ACG8Z3_002954"
     CDS             33..770
                     /gene="erm(B)"
                     /locus_tag="ACG8Z3_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0825175.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNDLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          290 a          137 c          124 g          236 t
ORIGIN      
        1 tttatagatg taatcacttc aggagtgatt acatgaacaa aaatataaaa tattctcaaa
       61 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      121 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      181 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      241 aattaaaact gaatattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      301 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      361 ttaaaaaagt ggtttttgaa agccgtgcgt ctgacatcta tctgattgtt gaagaaggat
      421 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      481 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca
      541 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      601 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      661 agtttcatca agcaatgaaa cacgccaaag taaacgattt aagtaccgtt acttatgagc
      721 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
      781 cgctttt
//
